LOCUS       DANKEF010000001       106950 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000001 DANKEF010000000
VERSION     DANKEF010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 106950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 106950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.001"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            211..603
                     /locus_tag="PWZ48_000001"
     CDS             211..603
                     /locus_tag="PWZ48_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2259700.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            631..1971
                     /gene="glnA"
                     /locus_tag="PWZ48_000002"
     CDS             631..1971
                     /gene="glnA"
                     /locus_tag="PWZ48_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HDL2259701.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3164..4774
                     /locus_tag="PWZ48_000003"
     CDS             3164..4774
                     /locus_tag="PWZ48_000003"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HDL2259702.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5066..5935
                     /locus_tag="PWZ48_000004"
     CDS             5066..5935
                     /locus_tag="PWZ48_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HDL2259703.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6100..7371
                     /locus_tag="PWZ48_000005"
     CDS             6100..7371
                     /locus_tag="PWZ48_000005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2259704.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7368..8663
                     /gene="rho"
                     /locus_tag="PWZ48_000006"
     CDS             7368..8663
                     /gene="rho"
                     /locus_tag="PWZ48_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDL2259705.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8762..9025
                     /locus_tag="PWZ48_000007"
     CDS             8762..9025
                     /locus_tag="PWZ48_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDL2259706.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9470..9919
                     /locus_tag="PWZ48_000008"
     CDS             9470..9919
                     /locus_tag="PWZ48_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2259707.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9976..11394
                     /locus_tag="PWZ48_000009"
     CDS             9976..11394
                     /locus_tag="PWZ48_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2259708.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSISTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11394..12254
                     /locus_tag="PWZ48_000010"
     CDS             11394..12254
                     /locus_tag="PWZ48_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HDL2259709.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12428..13201
                     /locus_tag="PWZ48_000011"
     CDS             12428..13201
                     /locus_tag="PWZ48_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2259710.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13348..13713
                     /locus_tag="PWZ48_000012"
     CDS             13348..13713
                     /locus_tag="PWZ48_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HDL2259711.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13861..15069)
                     /locus_tag="PWZ48_000013"
     CDS             complement(13861..15069)
                     /locus_tag="PWZ48_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2259712.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15088..15366)
                     /locus_tag="PWZ48_000014"
     CDS             complement(15088..15366)
                     /locus_tag="PWZ48_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259713.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15520..16215
                     /locus_tag="PWZ48_000015"
     CDS             15520..16215
                     /locus_tag="PWZ48_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2259714.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16657..17454)
                     /locus_tag="PWZ48_000016"
     CDS             complement(16657..17454)
                     /locus_tag="PWZ48_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDL2259715.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17451..18635)
                     /locus_tag="PWZ48_000017"
     CDS             complement(17451..18635)
                     /locus_tag="PWZ48_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2259716.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18660..19907)
                     /locus_tag="PWZ48_000018"
     CDS             complement(18660..19907)
                     /locus_tag="PWZ48_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2259717.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20122..21867
                     /gene="dnaX"
                     /locus_tag="PWZ48_000019"
     CDS             20122..21867
                     /gene="dnaX"
                     /locus_tag="PWZ48_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDL2259718.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21887..22201
                     /locus_tag="PWZ48_000020"
     CDS             21887..22201
                     /locus_tag="PWZ48_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDL2259719.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22226..22822
                     /gene="recR"
                     /locus_tag="PWZ48_000021"
     CDS             22226..22822
                     /gene="recR"
                     /locus_tag="PWZ48_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDL2259720.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22886..23530
                     /locus_tag="PWZ48_000022"
     CDS             22886..23530
                     /locus_tag="PWZ48_000022"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDL2259721.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23545..23874
                     /locus_tag="PWZ48_000023"
     CDS             23545..23874
                     /locus_tag="PWZ48_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HDL2259722.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23888..24826
                     /gene="holB"
                     /locus_tag="PWZ48_000024"
     CDS             23888..24826
                     /gene="holB"
                     /locus_tag="PWZ48_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDL2259723.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24862..25685
                     /locus_tag="PWZ48_000025"
                     /pseudo
     CDS             24862..25685
                     /locus_tag="PWZ48_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
     gene            25678..26025
                     /locus_tag="PWZ48_000026"
     CDS             25678..26025
                     /locus_tag="PWZ48_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HDL2259724.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26094..26966
                     /gene="rsmI"
                     /locus_tag="PWZ48_000027"
     CDS             26094..26966
                     /gene="rsmI"
                     /locus_tag="PWZ48_000027"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDL2259725.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27075..28196
                     /gene="dinB"
                     /locus_tag="PWZ48_000028"
     CDS             27075..28196
                     /gene="dinB"
                     /locus_tag="PWZ48_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDL2259726.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28250..28852)
                     /gene="nrdG"
                     /locus_tag="PWZ48_000029"
     CDS             complement(28250..28852)
                     /gene="nrdG"
                     /locus_tag="PWZ48_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HDL2259727.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29167..31356)
                     /gene="nrdD"
                     /locus_tag="PWZ48_000030"
     CDS             complement(29167..31356)
                     /gene="nrdD"
                     /locus_tag="PWZ48_000030"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDL2259728.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31655..32449
                     /locus_tag="PWZ48_000031"
     CDS             31655..32449
                     /locus_tag="PWZ48_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2259729.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32439..34481
                     /locus_tag="PWZ48_000032"
     CDS             32439..34481
                     /locus_tag="PWZ48_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2259730.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34762..34911
                     /locus_tag="PWZ48_000033"
     CDS             34762..34911
                     /locus_tag="PWZ48_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HDL2259731.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34922..36436
                     /gene="dltA"
                     /locus_tag="PWZ48_000034"
     CDS             34922..36436
                     /gene="dltA"
                     /locus_tag="PWZ48_000034"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HDL2259732.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36433..37638
                     /gene="dltB"
                     /locus_tag="PWZ48_000035"
     CDS             36433..37638
                     /gene="dltB"
                     /locus_tag="PWZ48_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HDL2259733.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37683..37916
                     /gene="dltC"
                     /locus_tag="PWZ48_000036"
     CDS             37683..37916
                     /gene="dltC"
                     /locus_tag="PWZ48_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HDL2259734.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37919..39187
                     /gene="dltD"
                     /locus_tag="PWZ48_000037"
     CDS             37919..39187
                     /gene="dltD"
                     /locus_tag="PWZ48_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HDL2259735.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39328..40410
                     /locus_tag="PWZ48_000038"
     CDS             39328..40410
                     /locus_tag="PWZ48_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HDL2259736.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40410..41213
                     /locus_tag="PWZ48_000039"
     CDS             40410..41213
                     /locus_tag="PWZ48_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2259737.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41283..42023
                     /locus_tag="PWZ48_000040"
     CDS             41283..42023
                     /locus_tag="PWZ48_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2259738.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42034..43038
                     /locus_tag="PWZ48_000041"
     CDS             42034..43038
                     /locus_tag="PWZ48_000041"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HDL2259739.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43083..44927)
                     /locus_tag="PWZ48_000042"
     CDS             complement(43083..44927)
                     /locus_tag="PWZ48_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259740.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45139..45657
                     /locus_tag="PWZ48_000043"
     CDS             45139..45657
                     /locus_tag="PWZ48_000043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2259741.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45785..46468
                     /locus_tag="PWZ48_000044"
     CDS             45785..46468
                     /locus_tag="PWZ48_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HDL2259742.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46777..46850
                     /locus_tag="PWZ48_000045"
     tRNA            46777..46850
                     /locus_tag="PWZ48_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46811..46813,aa:Arg,seq:tct)
     gene            47009..47368
                     /locus_tag="PWZ48_000046"
     CDS             47009..47368
                     /locus_tag="PWZ48_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HDL2259743.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47491..49122
                     /locus_tag="PWZ48_000047"
     CDS             47491..49122
                     /locus_tag="PWZ48_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HDL2259744.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49184..49825)
                     /locus_tag="PWZ48_000048"
     CDS             complement(49184..49825)
                     /locus_tag="PWZ48_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2259745.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50011..50087
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50107..50415
                     /gene="rplU"
                     /locus_tag="PWZ48_000049"
     CDS             50107..50415
                     /gene="rplU"
                     /locus_tag="PWZ48_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDL2259746.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50442..50786
                     /locus_tag="PWZ48_000050"
     CDS             50442..50786
                     /locus_tag="PWZ48_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HDL2259747.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50799..51092
                     /gene="rpmA"
                     /locus_tag="PWZ48_000051"
     CDS             50799..51092
                     /gene="rpmA"
                     /locus_tag="PWZ48_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDL2259748.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51526..54333
                     /locus_tag="PWZ48_000052"
     CDS             51526..54333
                     /locus_tag="PWZ48_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2259749.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54524..55015
                     /locus_tag="PWZ48_000053"
     CDS             54524..55015
                     /locus_tag="PWZ48_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2259750.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55118..56107
                     /gene="manX"
                     /locus_tag="PWZ48_000054"
     CDS             55118..56107
                     /gene="manX"
                     /locus_tag="PWZ48_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDL2259751.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56140..56943
                     /locus_tag="PWZ48_000055"
     CDS             56140..56943
                     /locus_tag="PWZ48_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HDL2259752.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56964..57878
                     /locus_tag="PWZ48_000056"
     CDS             56964..57878
                     /locus_tag="PWZ48_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDL2259753.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57961..58335
                     /locus_tag="PWZ48_000057"
     CDS             57961..58335
                     /locus_tag="PWZ48_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HDL2259754.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58474..58719
                     /locus_tag="PWZ48_000058"
     CDS             58474..58719
                     /locus_tag="PWZ48_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2259755.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58891..59955
                     /locus_tag="PWZ48_000059"
     CDS             58891..59955
                     /locus_tag="PWZ48_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2259756.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60201..60626
                     /locus_tag="PWZ48_000060"
     CDS             60201..60626
                     /locus_tag="PWZ48_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2259757.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60619..61068
                     /gene="nusB"
                     /locus_tag="PWZ48_000061"
     CDS             60619..61068
                     /gene="nusB"
                     /locus_tag="PWZ48_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDL2259758.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61165..62019
                     /locus_tag="PWZ48_000062"
     CDS             61165..62019
                     /locus_tag="PWZ48_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HDL2259759.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62020..63357
                     /locus_tag="PWZ48_000063"
     CDS             62020..63357
                     /locus_tag="PWZ48_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDL2259760.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63362..63586
                     /locus_tag="PWZ48_000064"
     CDS             63362..63586
                     /locus_tag="PWZ48_000064"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDL2259761.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63588..64478
                     /locus_tag="PWZ48_000065"
     CDS             63588..64478
                     /locus_tag="PWZ48_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2259762.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64487..65305
                     /locus_tag="PWZ48_000066"
     CDS             64487..65305
                     /locus_tag="PWZ48_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HDL2259763.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65412..65861
                     /locus_tag="PWZ48_000067"
     CDS             65412..65861
                     /locus_tag="PWZ48_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2259764.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65879..67561
                     /gene="recN"
                     /locus_tag="PWZ48_000068"
     CDS             65879..67561
                     /gene="recN"
                     /locus_tag="PWZ48_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDL2259765.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67616..67732)
                     /locus_tag="PWZ48_000069"
     CDS             complement(67616..67732)
                     /locus_tag="PWZ48_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HDL2259766.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67805..68761)
                     /locus_tag="PWZ48_000070"
     CDS             complement(67805..68761)
                     /locus_tag="PWZ48_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2259767.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68932..69300
                     /locus_tag="PWZ48_000071"
     CDS             68932..69300
                     /locus_tag="PWZ48_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HDL2259768.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69470..69901
                     /gene="mraZ"
                     /locus_tag="PWZ48_000072"
     CDS             69470..69901
                     /gene="mraZ"
                     /locus_tag="PWZ48_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDL2259769.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69922..70881
                     /gene="rsmH"
                     /locus_tag="PWZ48_000073"
     CDS             69922..70881
                     /gene="rsmH"
                     /locus_tag="PWZ48_000073"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDL2259770.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70886..71287
                     /gene="ftsL"
                     /locus_tag="PWZ48_000074"
     CDS             70886..71287
                     /gene="ftsL"
                     /locus_tag="PWZ48_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDL2259771.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71288..73480
                     /locus_tag="PWZ48_000075"
     CDS             71288..73480
                     /locus_tag="PWZ48_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2259772.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGIAYGVYSVPGYNVTAKTGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73506..74468
                     /locus_tag="PWZ48_000076"
     CDS             73506..74468
                     /locus_tag="PWZ48_000076"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDL2259773.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74482..75843
                     /locus_tag="PWZ48_000077"
     CDS             74482..75843
                     /locus_tag="PWZ48_000077"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDL2259774.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75869..76957
                     /gene="murG"
                     /locus_tag="PWZ48_000078"
     CDS             75869..76957
                     /gene="murG"
                     /locus_tag="PWZ48_000078"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDL2259775.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76972..78192
                     /locus_tag="PWZ48_000079"
     CDS             76972..78192
                     /locus_tag="PWZ48_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HDL2259776.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78348..79673
                     /gene="ftsA"
                     /locus_tag="PWZ48_000080"
     CDS             78348..79673
                     /gene="ftsA"
                     /locus_tag="PWZ48_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDL2259777.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79696..80937
                     /gene="ftsZ"
                     /locus_tag="PWZ48_000081"
     CDS             79696..80937
                     /gene="ftsZ"
                     /locus_tag="PWZ48_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDL2259778.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80954..81631
                     /locus_tag="PWZ48_000082"
     CDS             80954..81631
                     /locus_tag="PWZ48_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HDL2259779.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81645..82244
                     /locus_tag="PWZ48_000083"
     CDS             81645..82244
                     /locus_tag="PWZ48_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HDL2259780.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82259..82546
                     /locus_tag="PWZ48_000084"
     CDS             82259..82546
                     /locus_tag="PWZ48_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HDL2259781.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82595..83377
                     /locus_tag="PWZ48_000085"
     CDS             82595..83377
                     /locus_tag="PWZ48_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HDL2259782.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83398..84105
                     /locus_tag="PWZ48_000086"
     CDS             83398..84105
                     /locus_tag="PWZ48_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HDL2259783.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84367..87165
                     /gene="ileS"
                     /locus_tag="PWZ48_000087"
     CDS             84367..87165
                     /gene="ileS"
                     /locus_tag="PWZ48_000087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDL2259784.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKSYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87214..88740)
                     /locus_tag="PWZ48_000088"
     CDS             complement(87214..88740)
                     /locus_tag="PWZ48_000088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDL2259785.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88755..89402)
                     /locus_tag="PWZ48_000089"
     CDS             complement(88755..89402)
                     /locus_tag="PWZ48_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HDL2259786.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89586..89915)
                     /locus_tag="PWZ48_000090"
     CDS             complement(89586..89915)
                     /locus_tag="PWZ48_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HDL2259787.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90092..90820
                     /gene="sfsA"
                     /locus_tag="PWZ48_000091"
     CDS             90092..90820
                     /gene="sfsA"
                     /locus_tag="PWZ48_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDL2259788.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90836..91849
                     /locus_tag="PWZ48_000092"
     CDS             90836..91849
                     /locus_tag="PWZ48_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2259789.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91849..93126
                     /locus_tag="PWZ48_000093"
     CDS             91849..93126
                     /locus_tag="PWZ48_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2259790.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93189..95891
                     /locus_tag="PWZ48_000094"
     CDS             93189..95891
                     /locus_tag="PWZ48_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2259791.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96043..96360
                     /locus_tag="PWZ48_000095"
     CDS             96043..96360
                     /locus_tag="PWZ48_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2259792.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96390..96710
                     /locus_tag="PWZ48_000096"
     CDS             96390..96710
                     /locus_tag="PWZ48_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2259793.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96818..98278
                     /locus_tag="PWZ48_000097"
     CDS             96818..98278
                     /locus_tag="PWZ48_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2259794.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98346..98567
                     /locus_tag="PWZ48_000098"
     CDS             98346..98567
                     /locus_tag="PWZ48_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259795.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98598..98780
                     /locus_tag="PWZ48_000099"
     CDS             98598..98780
                     /locus_tag="PWZ48_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HDL2259796.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98780..99193
                     /locus_tag="PWZ48_000100"
     CDS             98780..99193
                     /locus_tag="PWZ48_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HDL2259797.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99316..100497
                     /locus_tag="PWZ48_000101"
     CDS             99316..100497
                     /locus_tag="PWZ48_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2259798.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100568..100732)
                     /locus_tag="PWZ48_000102"
     CDS             complement(100568..100732)
                     /locus_tag="PWZ48_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259799.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100886..100959
                     /locus_tag="PWZ48_000103"
     tRNA            100886..100959
                     /locus_tag="PWZ48_000103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100920..100922,aa:Arg,seq:cct)
     gene            complement(101028..102167)
                     /locus_tag="PWZ48_000104"
     CDS             complement(101028..102167)
                     /locus_tag="PWZ48_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2259800.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGLEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102466..103101)
                     /locus_tag="PWZ48_000105"
     CDS             complement(102466..103101)
                     /locus_tag="PWZ48_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259801.1"
                     /translation="MDNRNEKLTKKEYLELIVEGGIGAIPYIGGVVQSLYFGSKNEKR
                     FKRIEKFYKNLNESLENIRDQIPEDVFNLENKDQLIGIFEGINDEVEKSKAQNKIDMY
                     KNLYKNCLLRINNASWDNEEYYLLVLNQLTSIEIQLLACLMSRGNENFTGNISMSGYS
                     QELIDGSLNRLSDFGLLEKTISSIVLGDVGKQNMGYKISHLGIQFINSTLT"
     gene            complement(103214..103849)
                     /locus_tag="PWZ48_000106"
     CDS             complement(103214..103849)
                     /locus_tag="PWZ48_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3221 domain-containing protein"
                     /protein_id="HDL2259802.1"
                     /translation="MDGIFGFTGIVLFIIGLIMLIVRFIKKTNKKTPMILLIIGIIFT
                     TVGFSLSSSNEANDNNKQESSSSDTQTTTSNSSNKSLTQIEEFLETNNHDWGAFLDSY
                     YSITPATEQNTAFTKYISGKTYTFEGTVIESMTTRIAIIADKEYDDKSWNDISATPKV
                     SYVIFAKDLNNADTFTKGDKVTFTGEISSRGSNIEKSYAQWDMINSKVSKK"
     gene            complement(103883..104344)
                     /locus_tag="PWZ48_000107"
     CDS             complement(103883..104344)
                     /locus_tag="PWZ48_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HDL2259803.1"
                     /translation="MEKEILIKSPGKTLIKVTDDSISIIRKGFINLVNQGIKGEKTIP
                     FKNISAVQLKKPGMSNGYIQFTLLGGNESRGGILAATKDENTVMFTKKYWNEMENLKK
                     YIEKQQSILDNNSKETQISSADEIKKYKELLDEGIINQEEFDFKKKELLGL"
     gene            complement(104474..104905)
                     /locus_tag="PWZ48_000108"
     CDS             complement(104474..104905)
                     /locus_tag="PWZ48_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2259804.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFSASHFEFSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(104923..105243)
                     /locus_tag="PWZ48_000109"
     CDS             complement(104923..105243)
                     /locus_tag="PWZ48_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2259805.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            105542..106318
                     /locus_tag="PWZ48_000110"
     CDS             105542..106318
                     /locus_tag="PWZ48_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HDL2259806.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            106333..106536
                     /locus_tag="PWZ48_000111"
     CDS             106333..106536
                     /locus_tag="PWZ48_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2259807.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            106552..106890
                     /locus_tag="PWZ48_000112"
     CDS             106552..106890
                     /locus_tag="PWZ48_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HDL2259808.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        34908 a        18263 c        22357 g        31422 t
ORIGIN      
        1 ataaaaacac taataaagga tagtacagta taataaaaaa taaaaggtat ctggtacgaa
       61 aatcaactag ataccttttt tgtgttctta tttttttgtt attttataaa aaatagagat
      121 ctcatgttag aaaacctaac atatgcagtt gacaatattt tttttaattg gtacacttta
      181 gccaacttat aaaacgaaag gggacgtctg atgggagaaa aggaattgcg ccgatctatg
      241 tccgtatttc caattggtac agttatgaag ctgactgatt tgtctgctcg gcaaattcgc
      301 tattatgaag aacaagattt agttcatccg gaacgaagcg aaggaaaccg acgtatgtat
      361 tcgctgaatg atattgatat gttgcttgag attaaagact atctttcaga gggattgaat
      421 atggctggga tcaaacgtgt atacgaaatg gcacaggcga aaaaacaaga agcaaaaaac
      481 aagaagccgt tgacagatca tgacgttcga caaatttttc gtgatgagat tttggcgcaa
      541 ggtgggttaa gcaaaacagg tcattatcag tcgcaaggat tgcaaagaca attctttgac
      601 tgaataaatt aaaaaaggaa gagggatacc atggtaaaga aacagataac aggtgaagaa
      661 atcaaacgaa tcattgagga agaaaacgta cgatttttac gattgatgtt cacagacatc
      721 ttaggtacga tcaaaaatgt agaagttcca gtgagccaga tcgacaaagt attagaaaat
      781 aaaatgatgt ttgacggttc ttcgattgaa ggatttgttc gaattgaaga aagcgatatg
      841 tatttgtatc cagatctctc cacttggatg atcttcccgt gggaaagtgc tcatggaaaa
      901 gttgctcgtt taatttgtga tatctataat ccggatggga caccatttgc cggcgatcct
      961 cgcgggaatt tgaaacgagc attgaaagat atgcgcgacc ttggttttac ctctttcaat
     1021 ttaggacctg aaccagaatt cttcttattt aaattagacg aagacggtgg gatcacaacg
     1081 accttgaatg ataaaggtgg ttattttgat tttgcaccaa cagatttagg tgaaaattgc
     1141 cgccgtgaca tcgtgttgga attagaaagt ctaggctttg aagtggaagc ttcacatcat
     1201 gaagtagcgc caggacagca tgaaattgac tttaaatatg cagatgtggt agatgcttgt
     1261 gataatatcc aaaccttcaa gctcgttgtc aaaacgattg cacgcaagca tggacttcac
     1321 gcgactttca tgccgaaacc gttatttggg atcaatggtt ctggtatgca ttgcaatatg
     1381 tctttgttca atgatgaagg aaatgttttc tatgataaag acggtgaatt ggaactaagt
     1441 gaaacagctt atcatttctt aggtggtttg ctgaaacatg ctcgtgccta cacagccgtt
     1501 tgcaatccaa cagtcaattc ttataaacgt ctcgttccag gttatgaagc gcctgtttat
     1561 gtcgcatgga gtggccgtaa tcgttcgcca ttagtacgtg taccagaatc tcgcggttta
     1621 tccacacgtt tggaattgcg gtcagttgat ccatctgcaa acccatactt ggcaatggca
     1681 gtattgttgc aagcaggact agacggtatc cgtaatcaga tcacaccgcc agcagctgtt
     1741 gaccgtaaca tttatgtaat ggatgaggaa gaaagagaag ctgcacatat ccaggactta
     1801 ccttcaacta tccataatgc tatcaaagaa ttgcgtaagg ataatgtaat gattgacgca
     1861 ttaggacaac atatcttctc aaattttgtt gaagcaaaac gtctcgaatg ggcggcattc
     1921 agacaaactg tttccgagtg ggaaagagaa caatatctag aactttatta aaacaggaat
     1981 accgtgaatt agaggctgtg aaatcagcgt tttgacctga gtattagaga aactaacgga
     2041 aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag ctgattgaac
     2101 accgtttagt ggagaggttg tgaaaaagct gccctgcaac aagtaataag aatagccaag
     2161 caaaaacagc ttttcttgtt tttgaattgg ctgaacttat taccgcagat gcaagctttt
     2221 gaacaccgtt taccaagagg ttttgaaaaa gacgtttagt ttcgagtatt aagaaaaatt
     2281 ttgtgaaaat ggctttctac atttttgcat aaatttggct taataccgag aaactggctt
     2341 ttgaataccg tttatccaga ggttgtgagc ctaccattta actccgaatc acaagaaata
     2401 atttcaaaaa cagcttctca tgtttgatgg atattgtgac ttgtgaccga ggagttggca
     2461 ggagaacacc gtttatttag aaataaggag gcacgacttt tgtcgcatct ccttattttt
     2521 tacttatgtg attcgttacc ttcaatgaat ccctacaagg cacttttgtt gaaaaaaagg
     2581 tcatctttga aaaaattagt ggaattgttt tgtttttgat attatttaat agaaaagata
     2641 tgccaaaaat ccgagaactg tggactacgt tttttacgta aaggaggtaa ataagggtga
     2701 aagaaaaaga ttgtttctac gataagctat ataaactgtt gctgcaagta ggaaaaagta
     2761 gttctttgtt aggtgggata ttatctatca taatgtttgt tttatttttt tatctattat
     2821 ttcacttaaa atttttcagt aacgattttc tgatttgctt aggtttaacg ttagctatgt
     2881 ttatatcaag cttattatgt gggttaaatt ttttttgcac tcataaagga cttaagaaat
     2941 aatttctcaa aggatatcga aaaagaaaaa attgtaagcg gatagtattc atttgagtac
     3001 ttgactttcc cttagtcagc ttgtattata caccttgggc acccttttag agggtcagaa
     3061 gtttacagaa atttcggctc cctattctta ttgaagaata gggagctttg tttattgtct
     3121 gagacttttc ctgcacataa attaaaaagg agtttttgaa cttatgacaa aatatatttt
     3181 tgtgaccggc ggtgtcgttt catcaatcgg aaaagggatt gttgcagctt ctttaggacg
     3241 cttattgaaa aaccgcggct taaaagtaac tatccaaaaa tttgatccat acatcaacgt
     3301 ggatccgggg acgatgagcc catatcagca cggagaagta ttcgtaacag atgatggtgc
     3361 tgaaacagat ttggatctgg gccactacga acgttttatt gacatcaatt tgaacaaata
     3421 ctccaacgtg acgacaggaa aaatctattc tgaagttttg cgtaaagaac gtaagggcga
     3481 atacttggga gcaactgttc aagttattcc tcatattaca aatgagatca aagaaaaaat
     3541 catgcgagca gctaaaatga ctgattcaga tgtgatcatt acagaagtag ggggaacagt
     3601 aggggatata gaatctttgc cattccttga agcacttcgt caaatgaaag cagatgtcgg
     3661 tacagataat gttatgtata tccatacaac attgattcct tacttgaaag ctgctggaga
     3721 aatgaaaact aaaccaacgc agcacagtgt aaaagagcta cgtggtttag gtatccaacc
     3781 gaatattttg gtggtacgta cagagcaacc tgtttctcaa agcgtgaaaa acaaattggc
     3841 acaattttgt gatgtcgaac cagaagcagt tatcgagtcg ccagatgtag atacacttta
     3901 ctccatccca ttagttttac aagcacaagg aatggatcaa attgtttgcg accatttaaa
     3961 actagataca ccagaagcag acatgacaga atggaaagaa ttagaagaac gtgtcttgaa
     4021 cttgaagaaa aaagtacgga ttgcgcttgt cggtaaatat gtcgagttac cagatgcata
     4081 tatctcagta gttgaagcat tgaaacattc tggtttcgcc tataattcag acatcgagat
     4141 cgattggatc aaagcacaag aattaacgag agaaaatgta gaagaacgtt tgaaagacgc
     4201 agatggaatc ttggttcctg gtggatttgg tgatcgcgga gtagaaggga aaatcgaagc
     4261 gattcgttat gctcgcgaaa atgacgttcc gtttttaggt atttgtcttg gtatgcagat
     4321 ggcttgtgtc gaatttgcac gtaacgtcgt tggattagaa gatgcgtcat cagctgaaac
     4381 gaatccagat acagcaaaca atattattga tttaatggct gatcaggaaa acatcgagaa
     4441 tttgggtgga actttacgcc ttggattata cccatgtaaa atcaaaaaag gaacgaagac
     4501 ggctgaagca tatgatggag ccgatgttgt gcaagaacgt caccgtcacc gctatgagtt
     4561 caataacaaa tatcgtcaat tgtttgaaga acaagggcta gtcttttcag gtgtctcacc
     4621 agataaccgt ttagtagaaa tcgtagagct gccagagaaa aaattctttg tgggatgcca
     4681 attccaccca gaattgattt ctcgtccaaa tcgcccgcaa aaattaatca aagcatttgt
     4741 cggagcatca ttagcagatc acgaaacaaa ataatttttg aacttgatag aaagagaaaa
     4801 gtagcaagag aaggggataa gcaattttct tgtcactttt ctctttttct gatttgttat
     4861 tatttgtagg aaaaaagata gaaagcaaaa gacttttttc gattaaagcg ctattggata
     4921 ttttattttt tgcaaaaaac ttcttgaaaa ttttcatttt cattgatttt agtagaaaac
     4981 taccgaaatg tttggtaaaa tagggtcgtt ggttaagaat tgtcttaaca aatttagttt
     5041 tacaaaactt aggaggaatt ttattatgcc agtagtatca ggagctgaat ttttaaaagc
     5101 tgctcgtaaa ggcggttacg ctgtcggcgg attcaacaca aacaacttgg aatggactca
     5161 agcaatcctt gaagcggctg aagcaaaaca agcaccagta cttatccaaa catcaatggg
     5221 tgcagctaaa tacatgggtg gttacaaaat ctgtaaagat ttaatcactg atttagttga
     5281 agcaatgaac atcactgttc cagttgctat ccacttagac catggtgatt acgaagctgc
     5341 tttggaatgt atcgaattag gctatacttc agtaatgttc gatggttcac atctaccatt
     5401 tgaagaaaac ttgaaattag ctaaagacgt tgttgaaaaa gcacatgcaa aaggtatctc
     5461 agttgaatgt gaagttggtt caatcggcgg agaagaagac ggaatcatcg gtaacggcga
     5521 attagctgat attgaagaat gcaaacaaat ggttgctaca ggtatcgact acttagcttg
     5581 cggtatcggt aacatccatg gtcaatatcc agaaaactgg aaaggattag cttttgacca
     5641 cttacaagca attgctgatg ctgtaggtcc agacatgcct ttagtattac atggcggatc
     5701 aggtattcct caagatcaaa tcgaaaaagc tatctcaatg ggtatctcaa aagtaaatgt
     5761 taacactgaa ttccaattat catttgctgc agctactcgt aaatatatcg aagctggtaa
     5821 agataaagaa ggtaaaggat tcgaccctcg taaattatta gctccaggta aagcagcaat
     5881 cgttgaagat gctaaatctc atatcgactg gttcggttca gctaacaaag cgtaagcatc
     5941 cacataacag atacgatata taggaatgga gaacgacttt tgaagtcgct ctccattctt
     6001 ttttacttaa aacggcagac catgctcgtt tctttttttt atctatgata aaatacaagc
     6061 atatccatga aaaaagatta ataaacaagg tggaaacaga tgaaaaaaat cgtaatcaaa
     6121 ggaaaccgac ctttagctgg tgaagtaacg atcagcggtg caaaaaatag tgcagtcgca
     6181 ttgattccag ctgcaatctt agctgattcc ccagtgatct tggaaggtgt gccggacata
     6241 caagatgttc attcattaat tgaaatttta gagatcatgg gcgcaactgt ccatttttcc
     6301 aacaacatac tagaaatcga tcctcgagaa gtcgtatcga ttccgatgcc gcacggaaaa
     6361 atcaatagtt tgcgtgcttc ttattatttt atgggaacac tgcttggtaa atttggcgaa
     6421 gcggtagtag gcctgccagg tggctgttat ttaggcccta gaccgattga cctacacgtc
     6481 aaaggatttg aagctctagg tgctgaagtg acaaatgaac atggtgccat gtatttacgg
     6541 acagaaaata aaggattgcg tggcaatcgg attttcatgg acgtggtttc agtcggagca
     6601 acgatcaatg tgatgttagc tgctgtaaaa gcaaaaggac agacagtaat tgaaaacgct
     6661 gcacgagaac cggaaatcat agatgtggca actttactga ataatatggg ggcaaaagtt
     6721 cgaggagcgg gtacagatgt gatccgtatc gaaggagtcg aaacattaca tggctgtcgg
     6781 catttcatga ttcctgaccg catcgaagca ggaacctatc tagcattagc agctgcagta
     6841 ggaaatggta tcaaagtgaa aaatgtgatc tttgaacatt tggaaagctt tatagcaaaa
     6901 ttgcaggaaa ttggtgtcca tatgaaaatc agcgaagatg aaatcgaagt ttacccctcg
     6961 aaagaattaa aacccgcaaa catcatgacc tatccttatc caggatttgc cacagatctg
     7021 cagcagccac ttactgcgtt actattgatg actacaggca cttcagaaat catcgatacg
     7081 atctatgcga aacgtgtgaa ccatgtgccg gaactagcac gaatgggcgc tgatattacc
     7141 gtggaaggaa atatgatcat cgtgaatggt ccaaataaac ttcacggcac agaagtcgtg
     7201 gcttctgatc ttcgggcagg tgcttgttta gtgattgctg gactattggc agaagggaca
     7261 acgacgattt acaatgtaga ttatatctta cgcggttatg atcatattat cgagaaacta
     7321 acagccttag gggcatcaat tgaaatgata gaggaagtgg gagctgaatg agcgattatt
     7381 tgacaatggc agaactagag aacaaaacgt taaaagaagt ttatagttat gccaaagaat
     7441 ttaaaattcc ttattacagt aagatgaata aaaaggaatt atctctagct gtgattcgtg
     7501 cacaagcaga gaaacaagga ttttattata tggaaggaat attggatatc gttggtcaag
     7561 atggttacgg ttttttacgg ccaatcaatt atggaccaag tgcagaagat atctatattt
     7621 ctgcctcaca aatcagacga ttcagtttga gaaatgggga caaagtggca ggaaaagccc
     7681 gtccacctaa agaatcggaa cgttattatg gtttgatgca cgtggaaagc gttaatggaa
     7741 aagatccgga agaagcaaaa gaacgtccgc attttccagc cttaacacct ctttatccgc
     7801 aaagacagct tcaactggaa acaacaccgc aaagactatc tacccgaatg atcgatatct
     7861 tttctccaat cggttttggt cagcgtggat tgatcgtcgc accgccaaaa gcagggaaaa
     7921 cttctgtatt aaaagaaatc gcaaatggta tcaccgaaaa ctatccggaa gtagaattaa
     7981 tcgttctact gattgatgaa cgaccggaag aagtaacgga tctagaacga agcgtgaaag
     8041 gcgacgtggt atcttctaca tttgatctgc agccgcaaaa ccatactcga gtttctgaat
     8101 tagtattgga acgcgcaatg cgtttagtcg aagataaacg agatgtcgtt atcttgatgg
     8161 atagtattac tagacttgct cgggcctata acttagtcgt tccaccaagc ggacgtacat
     8221 tgagtggcgg gattgaccca gccgcattgt ataaacctaa gaaatttttc ggggcagcaa
     8281 gaaacatcga agaaggtggc agtttgacga ttttagcgac cgctttagtc gatacaggaa
     8341 gccgtatgga tgacgtgatc tatgaagaat tcaaaggaac tggaaatcta gaattacaac
     8401 tttcacgtga attatctgaa cgtcggatct tcccagccat cgatatcaaa aaatcaggta
     8461 cccgaaaaga agaattattg atgccaagtg aagaattaga agaaatttgg aaacttcgaa
     8521 aaaatatgac tggagattca ttagaatata cggaacaact gatccgcttt ttgcgtaaaa
     8581 caaaaaacaa tcagcaattt ttcaaagaat ttcaagatgt ctcctttggt aaaaaaaatc
     8641 cgaccagaaa tctgaaaaga taattgcaac cttgactgaa acatgttaag atgttaatgt
     8701 tgtaaatcga caaatcaact ctggttcagc aaatggagca gggcaaagga gcgaaaaaat
     8761 catgaaagaa aacatccatc cagattacca cccagtagta ttcatggatt caacaactgg
     8821 tttcaaattc ttgtcaggtt caacaaaaac ttcacaagaa acagttgaat gggaagacgg
     8881 taatacatac ccagtcatcc gtgtcgaagt aacttctgac tcacatccat tctacactgg
     8941 acgtcaaaaa ttcacacaag cagacggacg tgtggaccgt ttcaacaaaa aatacggtct
     9001 caaagacgca aacgctgcgg aataataaaa acgctgataa ctcaacgttt agcagactat
     9061 ctgataggga tagtctgttt ttttatgttt tttgccaatt taaaacttcg tttgatgctg
     9121 aaaaagtcgt aggatcataa tgcacaaaac aatgaaaaac tagtatatag ttttaattat
     9181 ccacttgata aaaacacctt atattaacga aatgaagagg ggttatttcg aagtaaaaaa
     9241 ccatttacct aaaattagaa gctaagcttt cctttcaagg aaggcttttt gttttgttat
     9301 agacttgcga ttgtttgtga gctagatcgt tcattttaat gacttataca cgcggatgat
     9361 gggtcttttt cctctgttca ttcataatat tgaacggtac agctagcgat gctatactga
     9421 cctcagttcc aagaaaacgt taacattttt tgtgggggag aggtttttta tgaggtttga
     9481 tcaattgatc gcagaagaac tgattgtttt cgatccatcg atagattcaa aaggatcatt
     9541 atttgagcga ataggggatg tgttgaaaaa aacaaataag gtaagaaatg ttcaaaaagt
     9601 cgttaaggca ctattcaaac gagaggagga gatctccaca ggaatagaag atggatttgg
     9661 gattccccat gcaaaaagca aatatgtgac aaaaccagca ctgtgttttt ttcatacagc
     9721 caaattaacg gactatagcg ggatagatga aatgccaatt gaatatattt ttgccatcgt
     9781 agttcctgaa aaatcagcag atatgcatct agagattctc agcacactct ccagacgatt
     9841 gatgaatgca gattttagaa agggaataaa agaagccact acgccagcag atatcatcag
     9901 acagattagt caagaatgaa aatagtagag tctttgtccg agtatcgata gtgaagaaaa
     9961 aggaggaaaa taatcatgaa aatcgttggt gtaactgctt gcccgactgg aattgctcat
    10021 acttatatgt cggcagaaaa attagaagta acagcaagaa atctaaacca tcaagcaaaa
    10081 ttcgaaacac aaggtgtaaa aacagaaaat cagttatctg aacaagaaat caaagaggca
    10141 gatgcaatca ttcttgcagt agataaagaa atcgaattgg accgatttgc cgggaaaaaa
    10201 gtaaagcgtg tctctacaag ccgagcaatc aaagaaccgc aagtagtaat agacgaagca
    10261 ttgagagata ttggcgtatt cgttgtgtct aatgaaaaag aaccagctgc aaatgagaaa
    10321 aagccgacaa tctataatca tttcatgaat ggtgtgaatt acatgcttcc atttgtgatc
    10381 gcaggcggaa tcatcatcgc actgagtttt gcctttggga ttacagcggc tgatcccaac
    10441 tcagcagact ataatgtgtt agcagctgca ttttcaagga tcggaggaga tacagcgttt
    10501 gccatgatgg ttcctgcgtt aggcgcgggg attgctacat cgattgctgg aaaagcaggt
    10561 tttgccccag gaattgttgc cggattactg gcctctacag gtggttcagg ttttcttggc
    10621 ggaatgattg gtggcgtgct agcagggtat atctgtgatt ttctggcaaa taaaattagc
    10681 gtaaaaaaag aattttctgc tatttatcag ctgattgtcg tccccttttt atcgatctta
    10741 attattgggc ttttgatggt atttgtgatt gaacaaccga tcacatgggt gttggatgct
    10801 ttgacaaatt ggctgaatag cttgggaaat acatccgggc tattgttcgg attacttatc
    10861 gggatcatga tggcagctga tatgggtggg ccgatcaata aatcgatttc gactttctcg
    10921 atcggtttga tgtcagctgg agtaaatgca ccgattgctg cttgtatggc tgcaggaatg
    10981 acacctcccc taggtattgc tttagccact ttattattca aacataaatt taccaaagaa
    11041 gaacggactt caggtcaatc atgctggatt ttaggcgctt cgtatattac agaaggtgct
    11101 ataccgtttg cggtatctga cccgatccgc gtgataccta gtttgatgac aggttcagca
    11161 gtcgctgcag ggatttccat ggcagtagga gttacttcaa tggcacctca tggagggatt
    11221 tgggtgatgc taattccaaa tgttatgaac aatttaccta tgtatgtctt ggcaattgca
    11281 tcaggtacag tcactacagc ctttatgctt ggactattga aaaaaacagt gaaagagaaa
    11341 aaacaagaca agtggacgaa aaatgaaaat ctaacaaatg aggtggaagc gtaatgttat
    11401 atacaatgaa agagttattg aaagtggcta aagagaatca atttgcagta ccagccttca
    11461 atatttgcag ctatgatatg atgaaagcaa tcatcgaaga agcagagaga ttagaagcac
    11521 cagtaatctt agaaattcat cctgatgaaa tcgcctattt aagtgatgag tttgttgcgg
    11581 cagtaagagc ctatgcttat acaagtaaac tgccgatcgt tatccacttg gatcatggcg
    11641 gtacgataga agatgtctta cgggcgattc gaaacggcta ttcttccgtg atgatcgatg
    11701 cgtctttgaa atcattcgac gacaatgtac agattacgaa aaaagtggtt gcgttagctc
    11761 atgcggtaaa tgtctcagtg gaggctgagc ttggaacgat tgggaataat ggttctgctg
    11821 aaggaggtgc tgaaacaatc atttatacag atcctgatca agcgaagatt tttgtgaaag
    11881 aaacaggaat cgatacactt gctgtagcaa ttggaacagc gcatggcttg tatccgaaaa
    11941 acagaactcc aaaattaaat atcgacttac tgaaagaatt gaaccaacaa atcgatcttc
    12001 catttgtttt acatggcggt tcaggtaatc cggacgcaga agtcagcgaa gcagtaaaat
    12061 acggagttgc caaagtgaat ttgagctcag atcttaaaag cgtctttttc aatgctttga
    12121 gagaaatatt gagtgaaaat ccatcgatgt acgagccgaa tgcaatttat ccagcagcta
    12181 ataaaaaggt acaagaagtg gtcaaacata aaatggaaat tttgaacaca gtaggcaagg
    12241 ctagtttata ctaaaaataa aaatgtgtgg tcaagagggt atgacaatag tcttgtcata
    12301 ccctcttaac cacacacgat gttcagaagt agttttttcg aaaaatttct tcttatttct
    12361 cgaagccaat cacttctgtc acaacctctt ttttcctgtt actatataaa gtaaatgggg
    12421 tgattttatg ttgaatgaag tggagaatag acagcaggag attgtgaatt atctaaaaaa
    12481 caaccaattt gcaagagtca ccgatttgat cgagttagta aattatagcg aagccacgat
    12541 taaacgtgac cttgtttttc tggaaaaaag tggattgatc cgtagagttc gcggtggcgc
    12601 catgcttatt gacaatcgaa aaattgacgt gccttacctg atgaagatca ccaaattaga
    12661 agaagaaaca gaaaaacgta agattgcaga tacagcttct tctttgattc gagatgatat
    12721 ggtcttattc ttggattcca gtacaacctc tttacactta gtcagaaacc tagcaagatt
    12781 tgatggtttg cagatcatta cgaatggcat cgtcacagct gctatgttga gtgaatatac
    12841 aacggcaaaa gtatctgtat tgggaggggc aattgttccg aaacgagcaa caatcaacgg
    12901 ttcaaaagcc tataacgatg cattgtcata ttatgcggat ttagcgataa tgagttgtcg
    12961 cgggctagat ttcagccaag gagtaacaga aactcatgaa ggagaggcaa tggtcaaaaa
    13021 agcttttaga aaacaatcag accggcttgc cgttttagtt acacagcaaa aaattgcaca
    13081 taaattcatg catcaagcca ttgcttttca tgaaatcgat tatctggtga caagtaaaga
    13141 actgacaaaa gaacagtatg cgaaagtaaa ggaacaccat atcgaatgtc tgtacggata
    13201 aattattacg tgaatgttct aagtggcttc ctcattttta gataggacct tatctttgct
    13261 cgcttctctc agccgatggt ataataatga aaaggttatc ttgttagata atcttacttt
    13321 tttatccagg agaaaggagt gcagaaaatg aaacatgtga aggcgttggt agtgaaggca
    13381 atcatgatct gggcgattct gtgggtcgtg ctgacaggtt tatacggtgt tagctttatg
    13441 gattcaacga ttgttggggt aattattgta gtcatgatct atgtgctagg ggatctgttg
    13501 atcttacgta aagtagggaa catcgcagcg acgatcgcag atgctggttc agctgcagtt
    13561 atcttgtggc tctatctata ttttatgaat gatacagtcg atatttggat gaaagtattg
    13621 attcctgctc tattgatcgg tgtggcagaa tggttcttcc acaagtggtt gctaagtcaa
    13681 ggcatcgtac cagatgaacg gaaaatggaa taaatcaaca gttaaactat tcttatcgat
    13741 aaatgaaagc tttaaaaata aatagatgtt taattaagaa taatcataaa ctatttttac
    13801 tcaaaaaacc gctcagatta ttttcagatc tgaacggttt tttttatcta ttcatcaaga
    13861 tcaaatttca tttttcaatg attcaatcaa ttcgccctct gcttcatgac tatgttttcg
    13921 gccattaaca agatagagtg ctgtacatat caatggaatg actccggcaa tcacatacaa
    13981 tcctctaaat gaagtaagag attttagcat ccccatcaaa taaggaccaa cgcctaagcc
    14041 taggtctaaa ccaataaagt aggtagataa agcaatactg attcgatgtt cgcggacgat
    14101 ttttaaacaa acagcctgtc catttgacat aaacgtacca taacctaggc cgactaatgc
    14161 tccagagata agcaatgtca cactattcgt cgttacactc agtaaaaata atcctgctgt
    14221 taagaataaa tagctaggat acatcacgta ttgttctcct ttgacatcga aaatccttcc
    14281 agtcaatgga cgtgtaaatg tgatgaccaa agcatatacg acgaagaaaa atgacccagc
    14341 agcaactaag tggattactt ttgtataaga agataaaaat gataaaacac ttgaataaga
    14401 aagacccatc aaaaaggcaa ttcctgtaat gaaaagtgct ttcttttcaa taaaactatt
    14461 gatattccat gagcgaagag atattttttg ttcttcagat aatggaatat tttttacagg
    14521 aaagacaagg caggcaatcg taacaaagaa gaccagtaca attgaaaacc agataatgaa
    14581 acgaaaatca gtcatattca gcaaaatcat tcctaaaaat ggcccaacag cggcagctaa
    14641 actagtactt aatccataat aattgatgcc ttcccctttt ttagatgcag ggatatatgc
    14701 agtaacgata gcatttgtag ctgtagaaac tgtaccgtag ccgaatccat tcaagaatcg
    14761 aactaaatac aatgccccga tagttggcgt ataaagatat gctaatgttg aaaggagata
    14821 gaagatcgcg ccgcctctta aggtaaactt ccgaccaaat agttctaact gtttccccat
    14881 gaataagcgt gccagcaatg ttccgatgat atagatccca gaagcaaaac ctgcttggct
    14941 caagctggcg tgtaaggtgt cttgcgcaat gactgccatg atgaccatca acagataata
    15001 aatcaggtag acaataaaat tgatcaatgt gattccaata aaatatttat taaataattt
    15061 cttttccatg tatgttatac attcccctta agctgaatat gcttttaatt tttgtatttg
    15121 aacttctaca tcgtgataat tggatggata gcgatcaaca agttggcgaa taaataaatc
    15181 tcttgtagac ataaacagat ttaaaaattc ttcgcattcg tttgttggat tctcatccac
    15241 tgctttgatc aattcgatcg agttttgata cgcatcattt aaaaagacta aagaagcttt
    15301 catatcaaat cttgtttgat atgctgtttc taatcgatta atgattgtgt tcatccagta
    15361 attcatgttt tttccctcta ttcttaaaaa attttacttc tgcagtataa cgaataaaaa
    15421 aaaggaaaaa aatcgcataa gtgatggaaa aaggtactta acaaatgtta atatcattag
    15481 taaaatacct ttggacttta aaaagtttta aagtactgta tgatcttttt agatgaaagt
    15541 ttggatgggg gagcatcatt ggatagtaga gtcttaaagg aatatttgga taaaaatgat
    15601 tttccgatta tacgcaaagg atatcataaa tatttgacat tcgaagggat ggaagattcc
    15661 catacgtata tcttgaaaga gggaacggta aaagcaagtg ttatttcaaa tgatgggaga
    15721 gaatttaacc ttcgctatat caaagatctt gaaatcgtct ctcttttaaa agacgaatat
    15781 tcacaattca tcgattcgcc gtataatatt cgagttgaat cagaatatgc agagttttat
    15841 cgaatcaatc gtgtgcgctt ttggcaaaat atcaaccaag acccaaagct acaacactat
    15901 gtaaaagagt attaccgcta tcgtctgctt tattcgatga aaaaaatgca gcaaatgcta
    15961 ttaaacggaa agctgggagc tgtttgtacg caactttacg aattatatga tttattcggt
    16021 gtgcggacag aagaagggat gctgatcgat ttttcgatca cgaatgaaga aatcgccaaa
    16081 ttttgtggca tctcaactgc tagcagtgta aacagaattt taaaacaact gaaaaaagat
    16141 ggtattatca ccgttaaaga ccatcatatc gttattttga atatggactt gttagaagac
    16201 aatattatct tttgatgaac aaagaggttg tgaaatcagc gttttgacct gagtattaga
    16261 gaaacaaacg gaaaaatagt tcctcatatt ttgccgtttt ttgagctaat accgagggtc
    16321 agctgattga acaccgttta ccaagaggtt gtgagcctgc cgtttaactc tgagtcacaa
    16381 ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg tgaccgagga
    16441 gttggtagga gaacaccgtt taccaagagg ttgtgaaaaa gctgttctgc atcaagtaat
    16501 aagaagagcc aagtaaaaac agtctcttat gtttttaact tggctggact tatcaccgta
    16561 gatgtaagct tttgaacgcc gtttatttgc aacaaaaggg gctgtgacac gatttttgtc
    16621 acagcccttt ttttgcgaac attcatttag caatttttag aaacttctgc cgccaccgcc
    16681 aaaagtcccg ccaccgctag aatgggtagt acttccgcca ccgccgcttc caggaggtgg
    16741 gcttttgggg attcttcttg tcgtgacaaa agaatttgtc agacgatcgg ttttttcagt
    16801 taatttgata ctggattttt ctcggaatgg atatttgtag atgcctgatt tcaattgata
    16861 acgcgaaaca gtcacaaaat aaaagacaag actaaggata agagccaaaa caacagcaat
    16921 gatgatttct actgtcgtca aaaccttata gcgtgtaatt ttgccagttt cttcgtctac
    16981 acgataatgt cctcctggaa ctccgtcttc cacatatgtt gctgatttag acaggtaagc
    17041 ttttgcagca ttgtaatagg cagcatttgt catctgatca taaacatcat ctaagatcga
    17101 atcgattctg ctatcagtca aataatcgat catattacca gaagtcgaaa tgtagatttc
    17161 acgctgtccc atatctaata gcaatactga gccattttga tcatttccga ctgcgtctct
    17221 taagtaatta tcagcaaagt ctcttggttc ttctgaatta gatgtagtcg ttacgatcaa
    17281 gactcgtcct ttgatttttt cattcagctt ttctgcctgt tgttccaaag attggatttg
    17341 atctgttgtg aataacccag cctcatcatt taccgtagct gtatctgcat gagtagccac
    17401 cggccagcca acacttacta gcaaaagaaa gaataaaaaa aattttttca tcatatcaag
    17461 taacctccga tcagaaagag gatcgctagc ccagcgaaaa tcccaagagc ggtcagtccc
    17521 aattttttct ggctgatcgg caggacaccg ctgacctttc ctgtttgtcc attcattgcg
    17581 tagtagtaga cttttttact tgagtcattg cttcgataag taacaagcca taccggcagt
    17641 aatacgtaat tatttacttc gtttgtgatt gaagcagaag ttcgcacacc ggtcaatgtt
    17701 gtatatccat ttgcggtatc ttttaacagc ttctctgaat agtcttttaa ttcagattga
    17761 atctgtgatt tgattgcctc atattcgata tctctttttt ctgcttggaa cccggctaaa
    17821 tactggcttt taaaatcaat tgctttatct aacgggaaag gttgcacgcc ttcgaccatc
    17881 ttttgttgcg tattttttga taaggcattt tttactagtt ctttaaaaga aagcttgcct
    17941 gctcttgaaa cagcaaattg cttcgtttct gtgtattcga tgtcacctat ccgccatatg
    18001 cggataactg taccattcgc ctgcatggca ccatctactt cagcatctgt agcccaataa
    18061 gggaaataca cacctgtcaa cttatcgatt tgatccttat tgaaaaaagc tttgggaaca
    18121 aaccattttt tcttggtcca agcgagaaat ctgttgattg cttcttcttt ctcgacagca
    18181 aaaggaagta ctttatttgg caagaattct ccgcttaaac gtcccgatag tacgacggga
    18241 ttatggcagt agtaacaata agtcgcagca gttgtcgcat cagtcacgat ctctgcacca
    18301 cagttgggac aaagaaaaag atctatcgct gcggtattat ctgcagattc gtccgctgta
    18361 tctgaaaaac cgcctgcctc gtcaaccgct ttgcgctcgg cttcattcat atcggcaagc
    18421 tgttcttggg ctgtaaaagt aaaatcttcg gtaggtgaag gttcttcttt gctttcttcg
    18481 ccagccatac gcgcttcttt ttgcttttgc tcatattggc tgacttcttc ttctgtatag
    18541 atattcaggc agtattcgca atgaaatttt tggtctttgg gatcaaaagt caacggaccg
    18601 ccgcaattag gacatttatg tgtaagaaca tccatcagct gcacaccttt ctaaataaat
    18661 taattaacgg ggattccttt aaacggttta ccacagtttg gacagaattt agggatgccg
    18721 ttggaaagat ccacgatctc attgcattca ctgcatcgca tttgtagctt aggctgaacg
    18781 ttttctacag gtttggcagc tccgcagttg gaacagaatt ttccggaatt ttctgtcccg
    18841 caaactggac atgtccaagt atccgcattt ccttgtgcag tttgttgatt ttgtttttct
    18901 gcttgttgtt tgatttgttc ttggttattt tgagctgctt gagaaagata agaaccattt
    18961 gcattcattc ccatacctac acccataaag ccggtcattg ctcctgcttc attttttccg
    19021 gctgcttcca taccacgtgc aatggaacct tgcacgtatc cttcacgtac gctaggatca
    19081 cctagcatcg ccccttcgtt acgcatattg atcagtttca cagaatcatc ggtgtaagaa
    19141 atgctggcta cagccacaga aacaatctcc atcccacgta attcacgcca agaatcatcc
    19201 aatgcagtat ccatgtattt gcttaattcc aaactcttag atggtacata agaaatccgt
    19261 tgaccatctg cagacatctg attgattgct gattgtagcg ctgttaggaa ttcagctaaa
    19321 tactgctcat tgatatcatt gatttccact tgtgttttgt ttttagggat tgcgtttgta
    19381 taaaataaaa tcgggtcaac gacatgaata gaataagtgc catgtgcccg taagaataat
    19441 tctgcgttat agaaattgtc aaagtagttc aacggcgcgc ttgtaccgaa tttgattccc
    19501 tttatttctt ggagattgat ataaaaaact tgttgtttct gcggtgtgac gccgccaaat
    19561 ttgaatcggt caaaagtttc agcaattgct tctttcagtg ttccattgaa catggaagga
    19621 gctgcgtcat ttttaatggt gtaataacct tcctcagcgg tataatcgat gatttttcca
    19681 ccatcgacca aaagcatcat catgtttgga tagacatgga cgactgttcc gtctgttaaa
    19741 acatcttctg ttcctttacg attggagcca cgtttgtcat ctttgcggac tttgacacct
    19801 tttgtcatga ccgttgtatc actcatattg tctggttcga tgacttctag ccattgatct
    19861 gccagaccgc cgccaaccat acttgttgct gctttgataa gacccataaa aaattcctcc
    19921 tctgattcag ctagtgccaa acaattattg tttatttatt ataccatagg aagatgtgaa
    19981 acttctcgga ctagagacgg aattgaaatg gaaaattgcg tattgtttaa atacctgaaa
    20041 aagccgagaa ggatagtagt ttttttacct aatatcacgt ataataagag atagtgaatt
    20101 ttttaaaaaa gggggatcct tatggcatat caagcacttt atcgagtctg gcgttcgcag
    20161 cgtttcgaag acatcgttgg acaaaaagct gtgacacaga cactaaaaaa tgctattgtt
    20221 tcccacaaaa cctcacatgc ctacttgttt acaggaccca gaggaaccgg aaagacgagt
    20281 gcagcaaaga ttttcgcaaa agcaatcaac tgtccgaaca gtaaagacgg tgagccatgc
    20341 aacgaatgcg aaatgtgtcg atcaattact gctggtacgc aagaagatgt gatcgaaatc
    20401 gatgctgcca gcaataacgg agtcgaagaa atccgattta tccgtgatcg ggcaaattat
    20461 gcgcctacaa aggcagcgta caaagtgtac attattgatg aggtacatat gctttcaaca
    20521 ggagctttca atgccttgtt gaaaacattg gaagagccaa aagaaagtgt catattcatc
    20581 ttagcaacga ccgaacctca caaaatacca gctacgatca tttcacggac tcagcgattt
    20641 gattttaaac ggatcaatac ggcagatatt gtcgaacatc ttgaatttat cttgaaagaa
    20701 tcgactatca gctatgaacc agctgcattg aatgtgattg cccgttctgc agaaggcggg
    20761 atgcgggatg cattgagtat tcttgatcag gcaatctcgt ttagtgaagg aacaattact
    20821 ttagatgatg ccatgcaagt gacaggtagc ttaactgacg aaatgatgga cagttacttg
    20881 agtgcttgcg tgaaaaaaga agtaccagaa gcattggaaa cattgaacca aattctcgca
    20941 gccggcaaag aatcacgtcg ctttctagag gatatgttac aatactgccg tgacttgctg
    21001 atgtatcagc aagcacctaa cctagttgag gaacaatcga gtacgatcag aagtacattt
    21061 acagaattgg cacaaactgt ttcagcggat cagctgtttt caatgataaa gattttgagc
    21121 gatactcaaa atgaaattcg ttttaccaat agtgccgcta tttatttgga agtcgctaca
    21181 gtcaaacttg cacagctacc gtctgcttct gttccttcag cagatgtagc atttgcagat
    21241 gatcagatag taaaacagct acaacaagag attaaggaac taagaaacga aatcaaggaa
    21301 ctacgcagta atacaggaat tgagaataca aaagtacagc cggtacagca aacaaatcat
    21361 aaaaaacaga caagtaccta tcgtgtaccg acggaacgag tatataaagt tctgcgggaa
    21421 gcgacaagac agcatctgaa ccaagtaaaa gaagtatggg aagacttgct gatgagtcta
    21481 tctgtcactc agcgagcaat gcttaaagca agcgaaccag ttgcagctgg acctacagga
    21541 ctagtgattg cgtttgatta tgagattgtt tgtcaaagag cagcaaatga cgaagaattg
    21601 cagctttcgg tacacaacaa cttaagccga atgatcaaag attatgcgcc tgatcctgtt
    21661 tatattacaa gagaaagctg gccagaactt cgaagaaatt atttgacgca ggcacaagaa
    21721 gagggaacag aaaactcttt tgcttctgaa gactcagaag gagaaatcga gttgcttcct
    21781 caagaacaag aagtaccaga agaaacactt gtagtaaaag cagaggaact gtttggctcc
    21841 gaagctgtta cagtggtaga tgattaataa aaaggagtag ataattatga tgcgtggaat
    21901 gggcaatatg caaggcatga tgaaacaagt acaaaaaatg caaaaagaaa tggcagaagc
    21961 acaagagctg ctaaataaaa cagagtttac aggtaaagcc accaatgaat tggtcaaagc
    22021 ggtttttaca ggcgatcgtc gcatgaaaga tatccaaatc aatgaagaag tcgttgatcc
    22081 tgatgatatc gagatgcttc aggatcttgt cgtaatggca gtcaatgatg cgatcgcaca
    22141 aattgatgtg gaaactgaga aaaaattagg gaaatacgca aaaggtttgc caggatttta
    22201 gttttaaact gaaggagtaa tgaatatgca atatccagaa ccaattgcga aactgatcga
    22261 cagctacatg aaacttccag gaatcggtca gaaaacagcg acaagactcg ccttttatac
    22321 gatcgatatg aaagatgaag tagtaaatga atttgctaaa tctttgttaa gtgtaaaacg
    22381 cgatttgcat ttttgtagta tctgcggaaa catcacagaa gaagatccct gcgaaatatg
    22441 tagaaatcct tcacgtgatc gaagcgttat cctagtggtc gaagagccga aagatgtaat
    22501 ggcaatggaa aaaatgcgag aatatcacgg actttatcat gttctccatg gtgtgttatc
    22561 accgatggaa gggacaggtc cagaagacat caatatttct tctctgttgc agcggctgca
    22621 tgatgcagat gtaaaagaag tgattatcgc aacaaatgca acaacagagg gagaagcaac
    22681 agctatgtat ctctcacgtc tgatcaaacc cgcaggtatc aaagtgactc gtctagcaca
    22741 tggtctgtct gtaggctctg atatcgagta tgcagatgaa gttactttat tgaaagctgt
    22801 cgaaggaaga cgtgagttat aaatttctaa ttaagtgttt tttcagatac aatataaaag
    22861 cagcacaaga gatgggggca acaatgtgaa cggactattt attacaatcg aggggcctga
    22921 tggtgcagga aaaaccagca tcttgaatga actttaccct cttttgaaag aagtagcaaa
    22981 aacagaaatc attcaaacaa gagaaccagg aggcattccg attgctgaag agatccgagc
    23041 agtgatcctt gatccaaaaa atgatcggat ggatgaacgt acggaagcac tgctttatgc
    23101 agcagcaaga agacaacatc tagtcgaaaa ggttctgcct gctctggcaa aagggaaaat
    23161 cgttttatgc gatagatttg tcgacagttc tcttgcttat caaggtgcag gccgacgcat
    23221 cggcgttacc gagattgctc ggttgaatga gtttgctaca gaaggtacta cgccggattt
    23281 taccctttat cttgacgtag attcagacac cggattaaga agaatcaaga aaaacagaca
    23341 aaatcaaatc gaccgtttag attcggaagg tttggaattc caccagcgtg tacgtcatgc
    23401 ttacttaaag ctggcagaag agaatccgga aagaatccac aaagttgatg caagaaaaag
    23461 ctttgaagaa gtgctgcaaa caagttatca tacaattatt gaacagtatc cgcaattttt
    23521 tgaaaactag gaaggtgaac gtttatgaaa attattttag cgattatcca agacaaagac
    23581 agcaaccgtt tatcaaatga attgattgat gcaaatatcc gtgccacaaa actatcatcg
    23641 acaggcggct tccttaaggc tggaaacagt acgtttatcg tgggtattga agatgaccgt
    23701 gtagaagaag cattggaaat catcaaaaaa acgtgtgaat caagaaagca atttgtatct
    23761 acacctgtta cactggatat ttcgatggat gggggcgtcc cttatccagt agaagtagaa
    23821 gtaggcggtg ccacagtctt cgtcttacct gttgaaggat tccatcaatt ttaggaggag
    23881 ctgagctatg aatcaagaaa taaacttggc tcaaatgcaa ccgatcgtgt atcagcagct
    23941 tcaacggagt tttgagcatg ggcgtatcgc tcatgcttat ctttttgaag gggagaaagg
    24001 aacaggaaaa catgaagtag gtatatggct tgcacagcac ctgttctgta cccaaatgaa
    24061 agacaatctg ccttgtggaa aatgcaacaa ttgtcagcgg atcgaaaaga aagaacatcc
    24121 agatgttttg gttattgaac cggaaggaca aaccatcaaa gtcgatcaga tcagacgttt
    24181 gcagacggaa ttcagtcgca gcggttacga atcaagaaaa aaagtttttt tgatcaaaga
    24241 agctgaaaaa atgaatagca gtgcggcaaa cagtttgttg aaattcttgg aagagccccc
    24301 tggagatttt ttagcggtgt tagaaacaga tgcagtagga agaattctcc ctactattca
    24361 atctcgttgt caagttctgc attttcaaga actttcgaaa gaagcactga tccatcgatt
    24421 gcaagaagag cagattccat tggaaaaagc aaaacttttg gctttcttaa caaacagttt
    24481 aggaaaagca gttgaaatct ctcaagatga atggtttaat gatgctaagg acttgattta
    24541 tcaatggttc gtttacttgc aaaagcaaga tactcaagcc tttatctatg tccaaaaaaa
    24601 attggtaaaa tcatttaaag aaaaaacaca acagtttaca ggattatctg tcttgatgtt
    24661 ttattatcag gaagcacagc gtcaagcagt agcaaacggc gaatatggca aagtgaaaca
    24721 gataaaccag ataattgaac ggatattgat cgcagagcag aaactacgca gcaatgtcag
    24781 ttttcaggct gttgcggaac agttcgtttt gcatacgatt ttttagcttg ccgcaaagga
    24841 tgatgatcaa ggaggatgga aatggtagaa gtagtaggtg tccgatatag agatgcaggt
    24901 catatctatt attttgcacc aggagactct gaatatgttt atagtgacaa ggtattagtc
    24961 gaatctcagc aatccttaca tattgctact gttgtccttc ctaaaaaaga gattgatccc
    25021 aatgaccttc ctgaagctat ccgtccgatc ttgcataaag cgtcacaagc tgaattagaa
    25081 aaagacaaaa aaacaaaaaa gatgcttatg atgcattgaa aatcgcaaaa gaaaaagtgc
    25141 agttgcgtga gttggaaatg aaaatggtca aagccgaata cacatttgat cgaaataaat
    25201 tgattttttc tttcacagcg gacgggcgaa ttgactttag agaattggta aaagatctgg
    25261 ccgctgtatt tcgtactcgt attgagcttc gacaaattgg tgtccgcgat gaagcaaaga
    25321 taataggcgg aattggtcca tgtgggcgtc cattatgctg ttcttctttt ttaggcgatt
    25381 tcgtgcctgt atcaatcaaa atggcaaaag atcaaagttt atctttgaat ccaacaaaaa
    25441 tttccggttt atgcggacgc ttgatgtgct gtctgcaata tgagagcgaa gaatacgaga
    25501 cagcgaaaaa agaactccct gactttggaa aagtaattgc tacacctgat ggtaaaggga
    25561 aagtcgtagg gttgaatctg cttagccgta tcgttagtat tcggctgact ggacgggaaa
    25621 atcctgtcga atatgaatgg gatgaattga aagaatttat ccaagtaggt gagatgaatg
    25681 gataagagat cattatacga cggattgaat caattagaat cagagttgag aaatacatta
    25741 acagaacttg ttgaaatgaa gcatgctttg catgagatcg tagaaaaaaa tacaactttg
    25801 gagatggaaa atcaacgtct gcgggaacac ctgcgggaat tgaaccaagt tgcacaagct
    25861 ccagctgaaa atgtgaagca ggaactttct aagtctcgta tgaatcttga aaaaatttat
    25921 gaagaaggat tccatgtctg ctacgattta tatggatcaa gaagggacaa tgacgagcct
    25981 tgtgcgtttt gtctagaagt gatctatcgc gaaagcggtc gatgaccaat aacaagagga
    26041 gactggagcg gagaaatact gctccagtct ttttattaag gagattgtga cagatgtata
    26101 atcaaaaaag tttcaaggga aatccggtat atggggattt atatcttgta ccgacgccaa
    26161 ttggaaattt agaagatatg agctacagaa gtgttcgtat gctgcaagaa tccgatctta
    26221 tcgcaagtga ggatacgcgt aatacacaaa agttattgaa tcattttgaa atccatacac
    26281 cccaaaaaag tttacatgaa cataattata aagaacgtgt ccctcaattg attgctttgt
    26341 taaaagaagg aaaaacgatt gctcaagtca gtgacgcagg aatgccttcc atcagcgatc
    26401 ctggacatga gctagtttta gcctgcataa aagaagggat ttctgtgatc tctatcccag
    26461 gacctacagc tgggatgacg gctttgattg cttcagggct agtgcctcaa ccatttttat
    26521 tttatggctt ccttccacgt aagaaaaaag aacagaaaga aacattgaaa aagctaaaag
    26581 aacagacagt tacgctgatt ttttatgagt caccttatcg gatcactgct acattgacga
    26641 atttcttaga aatttttgga aatcgccaag tcgttttgtg tcgggaattg acaaaggtcc
    26701 atgaagaata ccttagagga agtacggaag agctactcgc ttatatcgaa gaacatccag
    26761 taaagggtga atgttgtctg cttgtagaag ggaatttgtt cgaaatgcca gtgacggaag
    26821 ttgatgaaga gttgggaacg ttaaaggaac aagtagaagc aaagatcgct tcaggcgaaa
    26881 aaccaaatgc tgctatcaaa gcagtagcgc tgaaaaatgg gctgaaaaaa caagaagtct
    26941 accgtcagta ccatgaatta gactgagata gtataagttg tctataatga aagactaatg
    27001 aggaaatcag ctactaactt ttcaaatctc ttgaacgcga acgaatgttc ttgtataatg
    27061 aataagaggt gaggatgatg gagctgcaat ttcctttaca taatgataca tcaagaaaaa
    27121 tcattcatat tgatatggat gcttttttcg cttcggttga agaacgagat catcctgagt
    27181 ttcgcggaca tccattagta attgccagac accccagtga cactggtggg cgtggagtag
    27241 tgactacagc aaattatgaa gcaagaaaat tcggaattca ttcagcaatg agtgcacaaa
    27301 aagcgtatga gctttgtccg caagccatct ttaagcctgg cgaccatcat aaatacagcg
    27361 atatttccaa acaagtacgg gaagtattct atcgttatac agatattgtg gaaccagtta
    27421 gtatcgatga agcgtatctt gatgtgaccg aaaataaaat caattgcaag tcagccatca
    27481 aaatcgcccg aatgatccaa gcagatattt gggagagtgt ccagctaaca tgttctgcgg
    27541 gagtaagtta caataagttt ttggccaaat tagcatcaga ctatcaaaaa cctagaggaa
    27601 tcacggtagt tgcccctcaa gacgctgtgc catttttaaa agctttgcca attgaaaaat
    27661 ttcatggggt agggaagaaa actgtaccta gaatgcatga gctagggatt tatacaggaa
    27721 aagacctata tgaatgcaca gaaatgatgt tgattcgaaa ttttggaaaa atgggctatt
    27781 ctctttatcg taaagtcaga ggaatccatg attctccagt aaacgttaca agagagcgaa
    27841 aatctgtggg aaaagagcat acttatgggc agccgttgca aacggaagaa gcagtgctga
    27901 ctcagttgcg ccaactagct gaaaaagtag aagaagcact gagaagagtc caaaaacatg
    27961 ggaaaacagt agttttgaag gtcaggtaca cggactattc tactgtgacc aaaagagtga
    28021 cattgccaga atatatctat aaaaaagaag cactctttta tcaagcaagc cttatatggg
    28081 aggaaatctt gggagtagaa aaaggaatcc gattgttagg gattactttg acgaatctgg
    28141 acccgatgac ttatgaaaac atcgtccttc cattatggga aaatcaagaa atataaaaac
    28201 agctttgaga gttgaaaccg gaaatccgtt ttctctccca aagctgtttt tatatcaagt
    28261 cttgcttttt gatttgttca taggtttctg ttgcatccgt acatttttcc caaatcacaa
    28321 cttgattgga ttctaaagat ttttggacgt caatgatccg ctggttgcta cttccgcgaa
    28381 attgtagttt taggtcacgt ttgcttaatt caaagcgtcc gtcaacaagg atatcgattt
    28441 gcgaaaggag ttctaattta tcttctgaat cttgtagcaa ttcatcaaag gtatagcctg
    28501 tccatgacca gatatctttt gtcgtaccga actcttttcg gatacgtcgt acgacttgca
    28561 gacaaacatc tgtattcaaa aaaggttcgc caccaagtaa agttaatcct tgcacgtaat
    28621 cgttgcgcaa gtcagcgatg atttgttctt ctaattcagg tgtgaatggt ttgccatatc
    28681 ggaaattttg tgcagctttg ttgaagcagc cttcgcaggc aaataagcag ccactaacgt
    28741 aaagactgtt gcggacacct tcaccatcga caaaattaaa cggtttgtag tcagcaatat
    28801 agttttggct gtagtctttt gctaaccatt cttttggttt tggatttctc ataaaaaaac
    28861 ttctttcatt taaggttcaa agatatagct attgaagata aaatctactc gaccacataa
    28921 ataagaacaa actgacctaa atccattgtt caagaaagct tgttcttatt ttgatagagg
    28981 gcgttcaaag atacaaagcc atcgaaagag ggtggtgaaa aaagacttgt catagcctct
    29041 tattctgaat aaacggtgtt caaaagcttg catctgcagt aataagccca actaaacgaa
    29101 aaatatgagg aactattttt gtttagctat tcttattact tgatgcagga cagctttttc
    29161 acaacctcat ttcatgtgtt tcacacgtga ggaaatttct ttatggcggc catgtaccat
    29221 cggacgagct tgcggatttc ctaaatagcc acaagtacgt ttgactacat cacatgtttt
    29281 tggatcatga tttccacatt ctggacattc gaaccctcgt tctgtcggtt tgaaatcacc
    29341 ttcgaaacca cattgatagc aatgatcgat tggcgtattc gtaccaagat acccgatttt
    29401 gtcatatgca aaatcccata cagcttctaa agctttcgga ttttgttgga gcatagggta
    29461 ttcacagtag tggatgaatc cacctgaaca atattttgga taatccttct caaattccaa
    29521 tttttcaaat ggcgttgggt ttttacgcac atcatagtgg aaactgtttg tataatattc
    29581 tttatctgtg atattttcga tgatgccgaa tttttctgta tccaaacggc agaaacggtc
    29641 agttagactt tcacttggag ttgaatagac actgaaatga taaccatatt catcccccca
    29701 tgcatctgca ttagctttta attctttgac gatatcaagt gtgaaagctt ttgcttctgg
    29761 gtttgtttcc cattcgccac cgaagaaagc tgaagcaact tcatacaatc caatgtagcc
    29821 taacgataca gtagctcgac ggtttttgaa taattcattg acagaatcgg tacgtgaaag
    29881 acgtttcccg aaagcaccat acatatataa aattggcgca tttgctggaa tggcttcttt
    29941 acaacgttct acacgataga gaagcgcatc tttcatgatt tctaagcggt cagacagtaa
    30001 ctgccagaat ttattctgat ctccttttga ttcgatcgcg attcttggca aattcaatgt
    30061 aacaacacct aaattcattc gtccgacatt tacttcttga ccattttcgt ctttccaccc
    30121 ttgcaagaat gaacgacagc ccataggtac tttgaaactt ccagttaatt caacgatttt
    30181 atcgtaattc agaatatcag gatacattct tttcgttgca cattccaatg ctagttgttt
    30241 gatgtcgtaa ttaggatctt cagggtttaa gttcacacct tttttcaaag tgaagatcag
    30301 tttagggaag atagctgtcc gcttttcact ccctaatcca ttgattcgaa tctgtaaaat
    30361 tgctttttga atttctcttt caaaccagtt ttctcctagt ccaaatccta atgaagtgaa
    30421 aggagtttgt ccatttgaag taaataaggt attgatttca tattccaaac tttgcatagc
    30481 atcgaagata tcttttttgg tttttgcttt tgcatattct ttttggcgtt ctggcgaatc
    30541 gatccattct tccgcgtctt ttagatgttt tttatagttc aattctgcaa aaggagcaag
    30601 caattcatct gttcggtcgg cagaacagcc accatattga cttgacgcta cattggcaat
    30661 aatctgtgag atttgtgctg tggcggtctg gatagatttt ggcgattcga cttcggcatt
    30721 gccgattttg aacccattgt tcagcatgcc tttgaaatca atcaagcagc aattcgtcat
    30781 cggtgtataa ggatgataat ccaaatcatg gtaatggata tcgccttttt gatgcgcatt
    30841 tgctatatgt ggtggcaaca ttttcagtcc gattgatttt ccaacgatcc cggctgtcag
    30901 atctcgctgt gtattgaaaa cattactgtc tttatttgcg ttttcgttca cgacagtttg
    30961 gtccttgttg attagctttt caatcgaaaa gttgatatct gttgctttac tgcgagcaaa
    31021 atctcgtttg gtacgataat tgatgtactc ttctgctaat tcatattcgt ttttttctag
    31081 taatatatgt tccacgatgt tttgaatctc atagattttg acattttcag caaaccggct
    31141 tttgatttct tggacaacca agtctgcaat catttgaatt ttttcataag taagtggtga
    31201 taatgagccg tgaattttct tttctgcttt gatcagtgct tcattgatct tttgttcatc
    31261 gaaatcaaca tggcggccat cgcgtttgat gactttcatc tgggatgtct ctggctgctg
    31321 agtaacgaca gttttttctt cataatttac tttcattatc ttcaactcct gctaaattgt
    31381 tcttgtctat cataaaacaa aatcggggaa ggagcaacta aatatcggct atatatagta
    31441 ttcaatgtgc tgtataattg gatttctata ctatatgtag tgtatatttt attgtgaaaa
    31501 aaggtgaaaa cttgtagctg gtggatttgt tttttgcata tttaattttg ctgaataaaa
    31561 taattgaata aaatttttta ttcagtccaa gtaaaagtga caaaaacata agagcaaatt
    31621 attcgtagat atgctactat gcaaataaat agaaatgaaa aatgaaagag gaggagaaga
    31681 attggaaaag atgatcgaag tgaaacatct aagtaaaagc tacggcagca aactttctcc
    31741 cgtcaaagcc ttagataata tctcattcac agtagaagaa ggcgagtttg ttgggattat
    31801 gggcccaagt ggtgcaggaa agacaacgct gatgaatatc ttgtccacga tcaatttgcc
    31861 tacgatgggc agtgtctcga tccaaggaaa agaaatcaca aaaatgaaaa acagtgagct
    31921 cagtgacttt cgtcgaaaaa aactaggatt catttttcaa gagttcaatt tgatcgatac
    31981 cctaaatgca aaagacaata tcctattgcc cctagcagtt gaacggatga ctaaagagga
    32041 aatggaaaag agagtaagac atgtagctca attgttgaat atagaagaac tgctcaaacg
    32101 gtatcccgat gaactttctg tcggacagag acaacgaatt gcagctgcta gagcacttgt
    32161 tgttcagcca caagtgattt ttgcagatga accaactggc tctttggact cgaagtcggc
    32221 tacagaattg ttgaactact tgaagaccat gaatcaaaaa gaaaaagcaa cgatactgtt
    32281 agtcacccat gatccatata cagcaagcta ctgcgataga attttattta ttaaagatgg
    32341 tgtgattttt tctgaagttg tcagaagagg gacaagaaga gagttctttg agaaagtaat
    32401 agacatgcag gcgacgattg gcggaggagg aaaaatgaat gctgtataaa ctagccgtta
    32461 aaagtttttt tcggcagtca cgaggctatc ttgtttattt tttcagtttg actttatccg
    32521 taatgatcta ttattccttc agcgcaatga cctacgatca atctttagtc agaagagcaa
    32581 gtcaagatac ttcgatagat ggtggattga gttttggtgg atttatcatt actgttgttt
    32641 tgcttttttt cgtattcagt gccaaccgat ttttcttgaa tagaaggcaa aaggaaatag
    32701 gcatctatca gctttttggt atgaataaac tgcagatatc aggtatatat gtgttagaaa
    32761 tcatgataat tggattgttc gcctgtattt ctgggatatt actcggtatt attttttcca
    32821 aactcttttc aatgattttg gttcgaatga tggatatgga tcttactagt ccatttttta
    32881 tctccattcc atcagtagta gatactttat ttgctttttt catcatcctt ttagcagtat
    32941 ctgtatattc aatatggaaa atatggcgct atccaatgat tcgaagtttt ggagaaaaag
    33001 aacaagcaga tagttcaaca atgcgcttca ggacaaggca ccgattactc gcagtcatcg
    33061 ggttactact tttaacgagc ggatattttg gagctagtca ttttagggaa atcacttcat
    33121 ggatgatcag ttatcattat cttgatacat tagttttctt ttttcctttt ttaatttttt
    33181 tcgtttgtgt ggtcggtact tatttgtttt tctgttatac atttcggtta tttttgatca
    33241 tattgagtaa atggaagatt tattactatg gtatcaattt tttgataatg ggtaacacgc
    33301 aagttcattt gctcaaaagt tggcgtacaa attctctgat tacagtcatt ctcggaattt
    33361 ctttaatgac aattggcggc acggctagta tctctactat tttatcctac caggagaaag
    33421 tttcctcccc gatggactat cagtttgatg ttgagacaga gaaaaaaatc aagccgattc
    33481 tagcagagga agggcagaag ataactaaag aaatcacctt gcattataaa gtcattggga
    33541 gtatctatag tcaaaatctc gaagaaccta tgtttcaaac tgctaatctg atcacggaaa
    33601 cacaatatca agccttcaga aaagttaatc cagatttgcc gaacatttct ttgaaatcac
    33661 ctggtcatac tgttttgctt gatcaaatgc aaactctttt tcggaatatt tcattatatg
    33721 gatctagtat tggactgcca gataatcaag aattgaagat tcaacaaatg cttccaagtg
    33781 tattaggtga cgaaacaatg gtatatctaa gtcctgtact gattgtagat gataccgttt
    33841 ttgaaaaagc aaaaggtgtg gattatcaag tagtcaatgt agatgtatca ggtggaaata
    33901 atgaaaaaat agatgaacga ctgagcaatg acctgtcgat aaagtggctc aatgctgttt
    33961 attattctta tgatatcgta aatggaagtc tacaaggaga aattagcaca aagaaacttc
    34021 cggatgacca aatgaaggaa gcacaattta gccaagaaag ctctcgtttg aatttctctt
    34081 ccagctattc gaaattgcgt gcagttaatc ggcgaatcgg tatcaatatt tttgtcacac
    34141 tatttgtcgg gatgatcgtg attattgcta ctggtagtat tttgacagtc cggcaattag
    34201 cagaagcaga ggaagaaaaa gaaaattatc acctattgac taagctgggg attccacaaa
    34261 gaaggatcag acggatgatc ttcgagcaaa acgcacttac cttctttcca cccatgatat
    34321 tagggatcac tcacgcagta tttgcaatca atgtcttcac tcagtatata aaaactgccg
    34381 attactggtt ggcttatctt gtgagtggct tattgatcct tatctatctt ttgctttatg
    34441 tcattacgag tcagttatat tgtcggatta tcgaggaatg atatacggaa taagacaaga
    34501 taataaatag aactaaattt tgaataccat ttatttggaa taagaggttg tgacacgatt
    34561 tttgtcacaa cctctttatt ttttatattt tttaaaagta cgagaaatta taaagaaaaa
    34621 agcagtgatt tgatgtgcta tttttataaa agctatgtga caaaactgtt agtaaaaaaa
    34681 cggtttctgt aagctagaca catgtttctt tttgtcaaaa ttagttacac tttgtgtgag
    34741 gttatatgag gagggaatcc aatgaacaaa tggcgttcta atccaaaatt gaattattgg
    34801 ggaaaattca tcattcgaac aattttttat agtgcagtac tgctgttcct gatttatctg
    34861 tatcactata aaaatattca aggaggaact tttatctata acgaatttta ggaggaagag
    34921 tatggaaatc aaaacgatta ttgaagcaat cgatgattgg ggaataaatg aacctgatcg
    34981 agttgcttat caggcggatg agacgcatac ttttggtgaa ttgaaacaag catcagacgc
    35041 acttgcttat tatttagaaa aaaaggtaga aggcgatgga ccaattgtcg tttttggcaa
    35101 tttggagttt gaaatgattg tctcatttct aggggttgtc aaatctggcc atgcctattt
    35161 gccgattgaa gagcatacac ccaaggagcg tatcctatcg atctttcggg tggctaaacc
    35221 atctatggtc atcagtatcg gggactggat agaagatatt ccttcggttc ctgtcataac
    35281 aaaaaaagaa tttcaagaaa tcaagaagat accagtagga ttttatccca gaaatagcgt
    35341 aaaaggtaca gaaaattttt atattatttt tacttctggt actactggag aaccaaaagg
    35401 cgtacaaatc agtcatgata atctagtcag ctttgttcaa tggacgatgg aagactttgg
    35461 gatcaagcct ggtatgcatt tcttagccca agcgccattt tcttttgatt tatcggtatt
    35521 ttctatttat cctgcattag tttctggcgg aatgctgaag ccgttaaata aagcagttgt
    35581 tcaagacttt cgtcaattat tcgcgacatt gccagccttg aagttgaatg tgtgggtttc
    35641 tactccatca tttatggata tttgcttgat ggaacctaca ttcaatggag aaaatgtgcc
    35701 ggaattggac atgtttttat tttgcggaga agaattgacg aaaaaaactg cagaaagtct
    35761 acttgatcga ttcccaaatg cacggatata taatacttac ggtccaacag aagcaactgt
    35821 agctatttca agtattcaga ttgaccagca tgtactagat agttatgacc gactaccgat
    35881 tggctacgta aaaaaagaca cgaaagtttc tattgtccaa gaaggtaaac ctgtttcaaa
    35941 aggagaaacg ggtgaaatca ttattgcagg accaagcgta tcaaaaggtt acctgaacaa
    36001 tcccgaaaag acagctgctg cgttcttttg tcaaaatgac ttagcaagtt accatacagg
    36061 ggatgcaggt agactagatg aatatgggct tttattttat gaaggacgta tggatcagca
    36121 ggtcaaatta catggatacc ggattgaact cggggatatc gaacattatc tgctccaaga
    36181 caaccgaatc aaacaagctg tcgtcgtacc aaaataccaa ggaacaaaag tacagcagct
    36241 ggttgctttt gtcgtattag aaaacaaaac gaaacagccg gattttcagt tgactaaatc
    36301 tatcaaagaa caattactag aagttgtaat ggattatatg attcctcaga agttccatta
    36361 tgtcgatctg ctgcctcaga cagttaatgg aaaaatcgat cgaaaaaaat taacagctga
    36421 ggtgaatcct gaatgatgtt acctcatatg atcccttatg cagagccgtt ttattttttt
    36481 cttttagcca ttgcgcttgt accgatcgtt ctatctttat tgatatggga aaaaagatta
    36541 ccagtttatc aatcagcagt gacacttttc tttttatttg tcagctttgg aggtaattat
    36601 tggcaacagg gagcggcgct gattgggtat gttttgtggc agacgatact tgtatggggc
    36661 tattttgttt atcggcaaag gaaaaatcag acaggatggt tttttgccgc cgttatttta
    36721 gcgataattc cactgttttt aacgaaagtg actccttttt ttgaacatgg aaaagcatcg
    36781 ttacttggtt ttttaggaat ctcttattta acctttaaat ctgtacaaat gatcatggaa
    36841 ataagagatg gcatgatcaa aacatataag ccatttcgtt atatccagtt tctgctgttc
    36901 tttccaacaa tctcttctgg accaatcgat cgctatagga gatttgaaaa agatcagctc
    36961 catccgcctg ttcgtgataa atatgttgat cttttgggaa aaggcgtcca aaatattttt
    37021 gtcggttttt tatataaatt cattatcggt tattattttg gtcaagtact gcttcctgta
    37081 gtgggacgca ttgccttaca gcatggtggt atttcatggg cattagtagg ttatatgtac
    37141 gtgtacagta tgtatttgtt ttttgatttt gctggctata gcttatttgc agtcggtaca
    37201 agctacatga tgggatatca gacaccaatc aatttcaata aacctttttt atcatggaat
    37261 atcaaagaat tctggaatcg ttggcatatg acgctgtcct tttggttccg tgattatgta
    37321 tatatgcgat tgatgttctt catgatgaag aaaaaatttt ttaaaagcaa gatcgtaatg
    37381 tcaaatatcg gttattttgc tttgtttctt ctaatgggta tatggcatgg attgacgtgg
    37441 tactacatcg tatatggact gtatcatgct tgtctgatct gtctgacaga tgcatggatc
    37501 cgatttaaga agaagcataa agagcagttg ccttctaaca aatttacgca tggcttagcc
    37561 gtatttttga catttcaagc agtatgtttt agcttcctga ttttttcagg atttttagat
    37621 aaactttttt ttagctaacg aatagtgaca tattaattta tataacaaag gagaaaagga
    37681 aaatggaaga acaaatttta tcaatcttag aagaaataac aggaacagat gaagtaagaa
    37741 aaaatcgtga tattgatcta tttgatgaag ggttgcttga ttctttagca tctgttcaat
    37801 tattagtcga gctagacgga gagctggata tccaagtccc tgtatctgaa ttcgagcgag
    37861 aggattggag cacaccgaat aaaatcattc aacaagcaac tgcattgaag ggttgagtat
    37921 gaaaaagaaa ctttttgcta ttttcggccc ggttgtttta gctgttggct tactgtttgt
    37981 gttttttacc tcctcctatc aagtagatat ctctaatcca gaaacgatta gacgtgcctc
    38041 ttcttccatg tcagtgaacg tgttgaaagg agatgcgata aaaaattatg cattatctga
    38101 aaaacaatat attccttttt ttggctcaag tgaattaagc agaatcagtc catttcatcc
    38161 gtctgttctg gcagaaaaat accagcgaaa ctatcgtcca tttttgctgg gagcacccgg
    38221 gacacaatcg ttaagccaat atatgatgat gcgttctgca ggagatgcta tgaaaaacaa
    38281 gaaagtcgtc tttattattt ctcctcagtg gttcgttaaa aatggtgtaa aaactgatta
    38341 ctttaatacg tattattctg aacttcaaac atacgactgg ttgttttcaa tgaagaaagt
    38401 aacgcctgct gatcgctatt tagctagaag attattaact ttctcaaaag taaaagagaa
    38461 tgatacgttg acggcgattc tgcaaacgat caaaaaaggg aagctacctt tacctgaatc
    38521 tttgaatcag ctgcgcagcc aatggaacat gctgaagaga gaggatgaag tattcagcaa
    38581 tattgggttg aaagatcgac agcaaaaaat cgatcatgag tcaaaacgat taccaaaaca
    38641 ataccaagaa acagaattat ccattttagc aaatcaaatt ggagaacgag aaacgacgaa
    38701 taatccattc gggttgaaaa acgattttta tacgcatcgt atccgagccc acgagccaga
    38761 gctcaaacag tcacaaaaaa attgggatta tcgtttttcg ccagaatttt ctgattttca
    38821 gctggtcttg gatcaattag caaaaaacca taacgaagta ttgttcatca tcccaccggt
    38881 taacgagaaa tggagcgatt ataccggact atctcaagaa atgttacaag gatttgccaa
    38941 aaagatcaaa tttcaattaa atagccaagg attcaatcga atagctgatt ttgtcaatca
    39001 agcgggtacc aactatttta tggaagacac gatccatcta ggctggaaag gctggttggc
    39061 tgcagatcaa cagatcagac cgtttttaga agaaaatcat attactgcta gtaagtatca
    39121 tcttgacgat gcttttttta gcaaatcttg gcaacatcaa ataccagata aactacaatt
    39181 aaaataaaga aaacaggcga gtgttgctcg cttgttttct ttatttttga atattctgaa
    39241 taaaagataa aaataccaag tttgcctgaa caaaaatcat aagcaatggt ataatcacaa
    39301 tatgaatata gaaatggggg aaaacatttg gatacaaaag aagaacagct acttattgat
    39361 ttaacttctg caaaaggagt acctggaaat gaagaagagg taagagaagt attcagagaa
    39421 tatgccaaac cattcgctga tgatattttt tatgacggtt taggcagtgt gattgccaaa
    39481 catggtgctg gcggaggccc caaggtcttt atttctggac atatggacga agttggtttt
    39541 atggtaacaa aaataaccga aaaaggattc ttggaattcc aaactcttgg aggttggtgg
    39601 gggcaagtca tgcttgctca gcaagttgaa atcaaaacac gtgaagggaa aatcgtccac
    39661 ggtgtgattg gttccaaacc accacatgtt ttaacacctc aagtaagaaa taaaccttat
    39721 gaaataaagg atatgttcat tgatattggc gcatcaagcc aagaagaagc caaagagtgg
    39781 ggcatacgtc ctggagatat ggtcactcca tatattgaat acaaacgaat gaatggcagt
    39841 aaatatttac tggcaaaagc atgggataat cgtatcggga cagctgtttc tctgcgagta
    39901 ttagagaatc tatctaaaga agcacaccca aatgttttat tcgcaggaag cgatgtgcaa
    39961 gaagaagtag gcttgcgagg tgctcgaaca agtacacacc ttgtaaatcc cgatattgct
    40021 tttgctttag atactggtac agcaggagac actccaggaa tgacaccaaa agaagcagat
    40081 tcgattttag gaaaaggccc acagattttg atttttgatg catctatgat cccgcataaa
    40141 aaattattga attttgttat tgatgtagca gaggaaatgg atattccatt ccaatataca
    40201 gtgatttctg gcggaggaac tgatgcagga caaatgcatt tgacaagaga tggtgttccg
    40261 tctgtagcca ttaccgttcc tgttcgttat ctgcattccc atacatcagt gattcatgaa
    40321 gatgattatt tgaatacagt gaaactagtc acagaattag tgaaacggct tgatagtgaa
    40381 acagttgaaa aaattcggag ttatgaataa tgaataatag aatacctatt tctgaatatc
    40441 ggaaaaaagc acgagtttct ttggaaaatc aatggggaat caatacttgg cttgttttct
    40501 tatctataat atttacgagc gtcatccaga acttttttga cggtttcagt ccttttccgg
    40561 aagaaagcat gcagcagcac ttattgagtt ttctagtcga taatttactt ttattcgcat
    40621 tcaactatgc attgttttac attgcgctag tagttgtacg gggtggcaga ccaaaaagta
    40681 accttttatt ttcagttttc caaagaaagt attttggttc gatacttggc atcaatttga
    40741 tcaattcagt catcaactgg ctactcaaca gtttaatctt tctgcccatg tttttaatca
    40801 gcggagtgag tacttatatg cagctgcttt tcagttcaaa tgcggtacct acgggtgtca
    40861 tgccagaaga ttttcttgat atcagctttg ttttatcgat gatagggatg atttttgttt
    40921 cagcactcat catgagtatt gttggcggtt tattccaatt tgctgcatgg acaaagttcg
    40981 actatcctga tttgtcggtt attcaatgtt tgaaatatgg ctggttttta ttgaaagata
    41041 gaattggtac gtatattttg ttgcagctgt cttttatcgg ctggtatttg ctaggagcaa
    41101 tcgctttgtt catcggtctt ttgtgggtca ttgtttatgt gaatgtgtca gttgcagaat
    41161 tttatgaaca agcacggatt gaaaaaggag gtccagacgc ctattttact tagcatgtaa
    41221 gcagtttatt catcaaaaaa ataacttttg tggtaggata aatctttgag ggggaatgta
    41281 cgatgaaatt aactgtttta ggatgcttag gagcgtatcc ttataaaaat caagggacaa
    41341 caggttattt gcttcaatct gacgggttca atttgttgat cgatgcaggt agtgctacgc
    41401 tgatcaaatt acaagaacac cttgatccac ttgatctaga tgctgtgatc cttagccatt
    41461 atcatcatga tcatattgct gaccttggtg tattacagta ttactggcaa cttcatccta
    41521 agagaaatcc ggagacattg ctttcaattt atggacacac aaaagattcc attcactttg
    41581 aagaattgac gttggaaaat gtctcaaaag gacatcctta ttttgaagct gaagaattaa
    41641 aaattggtcc ttttctgatt acttttatgg aaacaatcca tccagtagta tgttacgcca
    41701 tgcgtatcgt agaagaaaca acggggaaag tattcgtatt tacaggtgat tcaggttatt
    41761 tggaaagttt cgctgatttt gcgaaagatg ctgatttgtt tctagcagat acctatcttt
    41821 ttgaaggaaa tgaacatcat catgcccatt tcacttcaaa agaagctgga gaacttgcga
    41881 aatcagctaa cgtaaagaaa cttgtactta cacacctacc tcagtttggt gatttagaac
    41941 aattgaaaaa tgaagcgcaa aaggcagcag gcaaagggat tgatatagaa cttgctgcag
    42001 ttgacaaagt gtttaacatt taggaggaag aacatgattt ttgtaccaaa tgaaaaccac
    42061 gacccaagaa tcaatttagc aattgaacaa tatttgctgc aagaaatgcc tttagatgaa
    42121 cctattttgc tcttttatat caatgaacct tcaatcatca tcggcagaaa ccaaaatacg
    42181 cttgaagaaa tcaatcgaga ttatgtggaa gaaaatggca ttcatgttgt ccgtcgctta
    42241 agtggaggcg gagctgtcta ccatgatttt ggaaacctga actttagttt tatcatgcct
    42301 gatgatgggg attcattccg tgactttgcg aaagtaacac agccaatcat ccaagcactt
    42361 catgaattgg gtgttgctgg ggcggaattg aaaggccgaa atgatctagt cattgatggc
    42421 atgaaatttt ctggcaacgc gatgtatgct accaacggtc ggatgtttgc tcatggaaca
    42481 attatgttcg actctgatat caatgaagta gtcaatgcat taaaagtaaa aaaagacaaa
    42541 atcgaatcaa aaggaatcaa atcgatccgt tctcgtgtaa cgaatatcaa accatttctt
    42601 ccagaagaaa agcaaaaaat gacaacagaa gaatttcgtc aagaaatctt gttgaaaatt
    42661 ttcggcgtag agtcagtaga agaagtaaaa acgtacgaat tgactgatga agattggaaa
    42721 aaaatcaatc gaatctctga ggaatattat cgaaattggg attggaatta cgggaagtct
    42781 ccagatttca atttctctcg ccaaaaacgt ttttctatcg gttcgattga agtccatttg
    42841 aatgtctcag aaggaatgat caaagaagca aaaatctttg gcgatttctt cggactagga
    42901 gatatctctg acgtggaaaa aaaattggtt ggacagaaat atgataaagc atctctatca
    42961 aaaatagtca gccaaattga tgtgaagaaa tattttggtg cgatagaacc agaagaattg
    43021 cttaccttac tttattgaaa ataaaaaata gcggaatcca catatatgga ttccgctatt
    43081 ttttatacag gattcgggta atttcgagag agttgaacag tgatccgatc aatgaaagta
    43141 tctttttcct gcgttagtgt tcctgtgtaa cgatccgcta cgtctttcat catcattaag
    43201 tcaaaacgtt cctgatcatc taattcttct gtgatgcctc gactaaatag gtttgtatcg
    43261 taatgtgcac tatgttcgca acaaatgacg aaatgttcct cttgttcgtg tatcgacaaa
    43321 taaatttttc gatacagtgg attttcgatg gcatgagttt ctctaaagga atgctctaaa
    43381 gcgtggatca aaagttgagc cagatcgtca tcgttgatag gaagttcttc gggaagatct
    43441 gcttggaagg ttatctgaat attccgtttg gcagctaact tctggtaacg cgctaagata
    43501 agatttgtca cctggtggtt gcaaaaagaa cacagcattt cgaaattttt tgcttcttct
    43561 aatatctttt ccacatagtt tttagctttt gtttggtccg tatgcattaa atcttgcaga
    43621 ttctccatct cttggacaaa ttccaggcga agtgtattta cttcttggat atgctgtcga
    43681 agctgctcat aatgttgctc caacaaatca tttttgattt ttaagaaatc tacttgtctc
    43741 cgatctttgt cggtatttaa gatgtgatcg attagattgt agctgataat aacgattaga
    43801 atgataatat ataaaatcgt agaccaattg atcgttgttt gatagatacc taatgccgtt
    43861 tggatgtaaa tcaagcaatg gataatggct accaagacga tccaaggtgt acaagctaca
    43921 taaaaacggt tattcttata tgcttcgcca atgcatgcaa gtgaagtgac taaagtgctt
    43981 ataacactag caatattagc aatcggcatt aattcaggaa gttctgcagg agtaaaagtt
    44041 gcataaagca gaacgccgat aaaaatcaaa agttggaacg tacaaaaaat tccgtaccat
    44101 tttttatagt aaaacattcg gctccaatag tagcagatga tgaaagctga cgttaagata
    44161 ataaataaat gagataaaaa agaatgaacg aatggttcaa aaagaatacc acttaaaata
    44221 tcttctgata cagcttcaaa tcctaatgcc aaagcaaagc aacttaagag aataagtgag
    44281 tattcaagac gtttttgttt atacatcaag tagatcgtat agaaaaaggt ccctatagcg
    44341 ataaaaacag cgattatcat cgtcaatact tgcggcactg attgtgaata aataaaacta
    44401 atcaatggtc cagttgttcc tagataaact ttcggtggca gtccagcata gttctgataa
    44461 ggagatgaga ctgtaagggt caattttttt ccgatataat ttgctggtaa atctatcaca
    44521 gccaatgaga gtccgggatt gctttcttcc tgataatctt tccgttggta taaaatcttt
    44581 tcgcctagat atacagtaat ccattgatgg tttcctttga ttagtaaatt tgcttcgtcc
    44641 aacggttttt caagtgtcgt ttccataatg aatgtttcgt ctttatccac ggtaggaatc
    44701 gtacgagtgt acttgctgtc aaacttaaaa tcaggatcat cttttgtata gaaaacccac
    44761 gaatctttta tttctttcat ttgattgctg tccgcaggag aaaaaataaa ataaaagaaa
    44821 taagcaattc ctgttaaaaa taacaatgat aatataatga ttatgctcca ctttatacgt
    44881 aaataaaaag attctttttt attatatatg ttattaaaaa aagacataaa ggtattcctt
    44941 tctaaaactt gtaatgttag ggtaaacgtt ttttaaacta acgtcaatta tttataataa
    45001 aaaatgagat aaagatgagg aaatagaatt agaaacatat aattattaaa gggaaacatt
    45061 tttacagcta taatttgaaa aaaatttatc gttattatat gattaagaag gattgaataa
    45121 agaaagaagt tgaaaacgat acctgattac aaaaaaataa cttttataag ttctataacg
    45181 ttgattatat ttttaattct tattcttctc gtcgtcttgg ttccccgaat ttttggtttg
    45241 actccttata ttattgagga caaagaaatg agtccatatt atgcacaagg aaatctgata
    45301 tatgtacgac cgaaaaatac aaatgagatt ttagtcggag atattattac atattatgaa
    45361 aattcaggaa aaaaaatctc gacgcgtcgc gtaatagccg ttgaaaacaa ccacgaagat
    45421 ttttatacaa aagctgatac aaagacttat attgagcctg gagtagtaaa aaagagaaat
    45481 attatcggta gtcccatctt tcagattcca tatattggac tagtcctttc aaaaacggca
    45541 tttgcatgga ttcaggggtt attctttacg attgcttttt gcctaacagg aatcacagca
    45601 tggaatacct ttgtagagtt gaaaaagaag cggagacgaa cacagaaatt tttgtaagaa
    45661 tgttaaaaac ataaatgtat tccagagatc caacgactct tctgaacatg aaacacgaga
    45721 aaacactcat gcagcttgta gatttagtca gttgttcgta taatgtgtga agaggaggag
    45781 tagtatgcgc aatgaaatga tgcatcgtcc tgtagtcaaa gaagaactat tggattttat
    45841 gcgtacccag caaaaaaaat taccgggtga gctaggcaaa ctggaagaag aagctcatgt
    45901 agcagaagta ccagttattc cccatgaaac agttgtattt ttgaaatttt tacttggaca
    45961 gcttcagcct gaacgaatct tggagatcgg agcagctatc ggtttttcat caagtgtcat
    46021 ggccacgaca atcggagaaa acgctcatgt tacgacaatt gatcgtttcg atgtcatgat
    46081 cgagaaagct aaaaaaaact atgaacgtct aggactgaca gataaagtga ctttgcttga
    46141 aggtcaagca gcagatatct tgccggagtt gtctggtccg tatgacttta tttttatgga
    46201 tagtgcaaaa tcaaaatata ttgaattttt acctgaatgc ttacgtttgt taagaaaagg
    46261 tggcgttttg atggtagatg acatcttcca aggaggaacg atcttgctcc cagatgaaga
    46321 gattccccgt ggcaaacgag ctatccatcg taaattaaat gaatttcttc gtgttgtgat
    46381 ggatcatccc gatttgacat cgacaatctt gccattaggt gatggtgtga tcttaatgac
    46441 aaaggaatca gaaacaatcg agttgtgata catgattatg gcagcctgta gataatcgat
    46501 tgaatcgagg gctcaattcg aacttgatgt aaaagtttct atatttgtaa gtcaattaaa
    46561 ggatacagga aatctaaata aacaggagta gcatgaagtc aatgtagctc ctgttttcaa
    46621 ttgataacct tagaacagct gaaggagaat gtctcgtttt aaaagattga tttagtagtt
    46681 gggccaaaat aattcattat ttgctaatta aataactatt tcgtattgac agaagcaatt
    46741 taattatcta taattattga tgttccgctg attcttgtca tagtagctca gctggataga
    46801 gcatccgcct tctaagcgga cggtcggggg ttcgaatccc tcctgtgacg ctaaaaaacc
    46861 gttagaaact gaggtttcta acggtttttc tttgtttttt gtgccaatat tgtttgtttt
    46921 caaaggcaaa taaaataaaa aaacatcctt atcgagtaca attataagga gcaaaaaaat
    46981 aaaactcatc agaaaagagg aagaagatat gacgatacta tctagaattt tagtgactct
    47041 agttgcaatt gaattttttt atatcatgta tatcgaaacg gtgagaactg attctgacac
    47101 gacaagtagg gtttttaaaa tgagtaaaga agagttaagc agaaaatctg ttcaaacatt
    47161 gtttaagaac caaggagtgt acaatggact attaggagtg ggactacttt atggagctta
    47221 tctttcttcc gcatctaaag aaatcacaag tatgctatta atatctattt tttttgttgc
    47281 attatatggc agtttgacta gcgatcgttg gattttactc aaacaaggtg ggctagctct
    47341 gttggcattg ctttctattt tcttataaat atagaaaaaa taagaattta tctaacagga
    47401 aactttgtga aaaatattac tttatctcat gaaatatgat attctaatga atggtatttt
    47461 agaaaatttc atgaaaaagg agttagatag gtgttcaaaa attatgtttc ctcactaaaa
    47521 aaagaatttt caggttatga tctttcaaga tttcagaaag atcttttggc tgggatcaca
    47581 gtagctgctg tagcattgcc tttagccttg gcttttgggg tatcaagcgg agctgatgcg
    47641 gccgcaggtt tgattacggc tatcattgcc ggtatcgtta ttggcggttt atcaggcggt
    47701 ttttatcaaa tatcaggtcc gacaggtgcg atggcagcta tcttgatgtc tattgcggca
    47761 acaaagggaa tgcaaggtgt attgcttgct acttttttag cgggtctatt tttacttgca
    47821 gcaggacttt tccgattagg aacactgact tcttttatcc cagcacccgt aattacgggg
    47881 tttacttcag gtattgctat cattattgca ttaggtcaga tcgataattt atttggtgtt
    47941 cactcggaag gtacaaacgt tattgaaaaa ctaagtagtt atcaaacgat aggatttccg
    48001 ataaacacag cctctttgat catgggaggg ctagtcattt tagggatgtt tatctatcca
    48061 aaaaaatggg cgaaacgaat gcctagttcg ctgctggcta tcattttggc aacagcagtt
    48121 atgctattgt tccatttacc agtagcaact gtgggtaaga ttccacaaac attgatcagt
    48181 agtaatcgtt tgaatatggg agattttagc ttttctgcct tgcaacaagt ggcagttcca
    48241 gctattagca ttgctttatt aggaatgatt gaaagcttgc tatgtggtgc ttcggctggc
    48301 cgaatggcaa ataagccctt agatagcaat caagaattaa tggcgcaagg aattggaaat
    48361 ctgttgcttc ctttctttgg cgggattcct gctactgcgg ccatcgcccg tacaagcgtg
    48421 gccatcaaat ctggtgcaca aactagatta gctggtatga tacatgcagt tgtcttactg
    48481 ctctccatgc ttgtttttgc gccagtgatg tcacagattc caatgccagc acttgcaggt
    48541 gttttgattg tgacggcttg gcgtatgaat gaatgggaaa cgatcaagga gctatttact
    48601 aataaatatt ggtcagcagt tctattgttt atccttacga tgatttgtac agttatcttt
    48661 gatttaagta ttgctatcgt gatcggggtg atcggcggct gtgtattttt tgttgtcaaa
    48721 agcgctgcta tcacgatttc attagaaacg atcgattggc aaagaatggg gctgccagaa
    48781 acggaaaagc tgaataactg gacagtagtg tatattagtg gtccactgtt ttttatgtct
    48841 tcagaaaaac tcaaaaataa attggaagaa ctaaatcatc acgatggtgt cattttctcc
    48901 atgcgaggcg taccaagtat tgatttgacg gcacgtgcag tttttgatga atttcaagaa
    48961 aaagcagagc aaagaggaca aacgattatc tatacttcct tgcagccaga agtagaaaaa
    49021 caactttctc acttgtggga acatacattc acgcatcaac atcaaacagt tgctcatgcg
    49081 ttgacagctt tacatgcgaa atatactgca attagccatt aaaacaatga aaaagaacag
    49141 acaagactgt aaactgaaga ttttatggtc ttgtctgttc tttttaatat tgctgtaaaa
    49201 gtgaagctag tacttgccag tcttgatgga taacaggtac aagggagcga ttgtagaaaa
    49261 tagctgctgg atgaaaaagt ggaagaattc gatagttttt ttctgaccat tcgtaggcat
    49321 ccttttgttc tgttaattgt aagacaggcc ctgtatagag ttgaccatga tttttggtaa
    49381 ccgtatattc tttgccaagt aaacgttgaa gaccaatgtt tccaagtgtc gcaattaatt
    49441 ttggctggac cttcatcagt tcgtaatcca atattggtgc atgcgcaaat acttcggaac
    49501 gagtaggtgt ccgatttgga taaacagttt ctgactgttg tgtccgttta ttgatccgat
    49561 gagtcacgcg atacgggcgg ctccttacag cactggtgat atacacctct tctcgtgtta
    49621 agccagttga agaaagtgcc tgcatcaatt cttttccagc ttgaccgcta aaaggaacat
    49681 gcgtatcaat ctctgttttc cctggggctt ctcccacgag catcagttta ggatgaagag
    49741 gtccttgccc agcgataaat ccttctacag gacggccgtt cattcttaaa gaagcttctt
    49801 gtatcagttc ttttgggtat tccatttatg ctcctccttt ttcttttatt ataagaataa
    49861 aagaaacaca atgcgactat tttatttggt agaactgtga ggatagaaaa gcagaagtat
    49921 tgcttgaatt tttgttttac tttttgattt taaatttagt tgactaacgg ttgctgttct
    49981 ggtaagctag tactgttgta attgtggact ccacagctac aaccgctcag aacaagtaat
    50041 aagcacagca tatgctgtca cttcggatgg cgagtctaag tatatcagaa ggaggtgctt
    50101 tatagcatgt acgcaattat taaaactggt ggtaaacaag taaaagttga agtgggacaa
    50161 gcaatctatg ttgaaaaact tgacgttgaa gcaggagaaa aagttgtttt tgacgaagtt
    50221 atcttagtag gcggagaatc tacaaaagta ggcgctccaa ctgttgcagg tgcaactgtt
    50281 gaaggtactg ttgaaaaaca cggcaaacaa aagaaagtcg ttactttcaa atacaaacct
    50341 aaaaaacatt cacaccgtaa acaaggtcat cgtcaaccat atacaaaagt ggttatcgac
    50401 gcgatcaacg cttaatagcg tttgagaaag aaggcctata tatgatcaaa ggaacattta
    50461 agcgaaatga cgcaggtcag atcgtttcat tcactttgac cgggcatgct gatgctggtc
    50521 cttatggaag tgatatcgtt tgtgcgggtg tttctgcgtt agcgatcagc actgtcaacg
    50581 ggattgcctc attagccggt tttgagccga ttgttgaaat gaatgaagaa gaaggcggtt
    50641 atctttatac cgaagtcact tcaggaatga cacaagaaca aaacaatatt gctgagattc
    50701 tattagaaaa tcttttatta ggattacaat cgatagaagc tgaaaactca gaatatatcc
    50761 aaattaaaac aattaatgat aaataggagg tgcagaccat gttgttatct atgaatctac
    50821 aattatttgc ccacaaaaaa ggtggcggtt ctacatctaa cggccgtgac tcagaatcaa
    50881 aacgtctagg tgctaaacgt gctgatggac aaacagttac aggtggatca attctttacc
    50941 gtcaacgcgg tacaaaaatc tatccaggtg taaacgtggg tatcggtgga gacgacactt
    51001 tatttgcaaa agttgatggc gtagtacgtt tcgaacgtaa aggccgtaac aaaaaacaag
    51061 tgtctgttta tccagtagct caagaagctt aattgaatct tatgattaaa gaggttgtga
    51121 caagactttt gtcacagcct tttttgttta taaaaataaa agttgtagtg gaggctacga
    51181 agtttacttt gtctttttac acttaatatt tttttgccaa tttggaatca ggatgttttg
    51241 ataactatcg ttttcaattg atggaagcta tattgtttgc tatcagtgaa ataattatat
    51301 ggtaaacagt gttctttttc ttgtttccat tataaaaatc gtatcgggat ttttcgttct
    51361 tttaaattga acatttctat ctatccgcgt gttatcaaca aaacaaaatc aggtaaatag
    51421 ttgacgaaac agaaataaat acatagtatt atgttttgaa gcctaagtac aatagtctat
    51481 agaaaaggag cgataccatt ggcaaaacgc attgaccgaa tttatatgta tgtcaaagaa
    51541 aaaaccgctc atttaacaaa ggcagaatat gatcaaggag taacaacaca ggaaattgct
    51601 gaagtgttag ggattcagcg aacaaatagc agtaaagatc tgaatcagct agtgcgcgaa
    51661 ggaaaactga taaagacaga cggacgacca gtacgttaca tatatcaaac actcgttacg
    51721 cacgtaaagc cagttacaaa acatgtagtc agttacaaag aaacgccggt ctctgaagaa
    51781 aaaaatacac tccaaataga tactaaggat atctttgcaa aaatcattgg agctaacggc
    51841 agcatgaaaa attcagtgga acaggcaaaa gctgcgattt tgtatccgcc aagaggatta
    51901 aattgtttga ttacaggacc taccggatca ggaaagactt attttgcaca cgcgatgtat
    51961 cattttgcaa aagcgcatca tgtaatagat gaggaaacgg aactgattgt tttcaattgt
    52021 gctgattatg ccaataaccc tgagcttttg atgagtcatt tatttgggta cgcaaaaggt
    52081 gcttttacag gcgcagatga agaaaaaatg gggattatcg atcaagcgga tggcggtatg
    52141 ctgtttttgg atgagattca ccggttgccg cctgaaggtc aagaaatgat cttttacttt
    52201 atggatcatg gaacttacag tcgtcttggg gaaacaacga aatcccatga agcaaatgta
    52261 cggatcgtag gtgcgaccac agaagaccca ggttcttctc tattagaaac ttttgttcgg
    52321 cgtatcccaa tcaatatcaa acttccggct tttgaaaaac gtcctgcaaa tgagaaagtc
    52381 gatttagtaa aaatcatgat tgcccacgaa gcaaaccgca cgcagcgaaa gatttctttg
    52441 acagaagatg tagcaaaagc attgatcggc agtgtgacct atggaaatat aggacaattg
    52501 aagtcaaata tccagttagt atgtgcacga ggatttctaa atcatatgca atcacccgaa
    52561 atctcgatca caatcgatga tttgacagaa gggattcgta gcggattgat tcaattagca
    52621 agcaaccgca gtgcaatggc agagctttct aaaatattgg agccaaagat aactgtagca
    52681 ccaaatgata cgatcatgaa aatccaatcg gactcctatg agttacctta taatctatat
    52741 gatatcatcg gagataaagc agctttactg aagtcggatg gtttggatca agaagcaatc
    52801 aatcatttca tttctacaga tatcaacatc catctgaagt ctttctataa agaccatggt
    52861 ttttctttta atgcagacag caaattagca gaatttgtag atccgaaaat catagaagtg
    52921 accaatcaga tttatgtaat ggtcaaaaga tctttaccat atgagtttca gcaaaacttt
    52981 atttatgcta tgagcttgca tatcagttct tttttgaaaa aaatcaactt aggagaagaa
    53041 cggcatacga atgataacat ccgtgagatg gcttttgatt ttccagatga atatgctgtt
    53101 gcgaaagaag tgcgtagtta tatcgaaacg tattttcaag tggagattcc agaaagtgaa
    53161 gactattact tgaccgttct actcgtctcg ttacgggcaa atcaagcaag cggcagaatc
    53221 ggtgtagtcg ttgcagctca cggaaacagc acagcaagca gtatggtcca agttgtccaa
    53281 cagttgttag atgcagagaa cgtacgagca gtagacatgc ctttggatat ggatccgaaa
    53341 acagcattgg accgaattga acgcaatgta caagaagtag atgaaggaag cggggtcata
    53401 ctcttagtag atatggggtc ccttgcgtcg tttaattcgc aaattcaaag agacactgga
    53461 attccagtac gtactgttga tatggtaaca acttctctag ttttggaaac tgtacgaaag
    53521 gtctcagtat tagggacaga tctagatatg ttgcatgaat cattgaaaaa ttttcgaggt
    53581 tacgcagaaa tatcttctgt agaatctgtt caggaagcaa aacctattca taagaaaaaa
    53641 gctgttttgg ctatctgcgc atctggcgaa ggtacagcac aaagaatcaa agaattgatc
    53701 gaacgagcgg taaacaagcg tcaagaaaca gaattagaag ttctagcttt gtcgattgtg
    53761 gaagtgaaag aagaactgcc agttatccaa aaagaatatc agatcatagc tactactggg
    53821 attactgatt tgaatatcca agccccattt attcctttag aacgttttat tgatcaaaat
    53881 atcgaagtca ttttggatca gttgctgatg gaatcagaat tggaggaaac tgaattcatc
    53941 tccctagatg aagagtcagc taaaaatacg tgtgttgagt ttatctctga taacttcatc
    54001 tttattaatg gtagtaagtt aatcgatcca atgtggcaat tttctacaca aatctctcag
    54061 acgacgggaa tcggcgatga agaatatgga ttcaaaatca acctagtgat gcacacagca
    54121 ggaatgatcg aacgtattat tcggaatgaa ccattgactg tggaagaaaa cgaattgaca
    54181 aatacaacga atgatcctct ttatagccaa ttggcagcct cagttgttct gttggaagat
    54241 cagataaaag taaaagtgcc gatagaagaa atgtattatc tgctacgtct agtccataat
    54301 cagttagata aaaaagaata cactgtgcca tgaatacaca aaaacgatac acttttatag
    54361 tgtatcgttt ttgtgtattg gttaagaatc ctttaaattc aattagtgta tcgtaaaaaa
    54421 gttggcacgc tttttgctta tagtagtgtg tgctaggagg tggttgttca cacagaaatg
    54481 ataggggttt ctgtgaagta gtgaggaaga aggagttttg cagatggata ttcgattggc
    54541 acgtatagat gatcgtttaa tccacgggca agtcgcaaca gtttggtcga agcaaacagg
    54601 tgttgaacgt atcattgtga tcagcgatgt agtcgctcaa gataaattga gaaagtttct
    54661 actaaaagaa gctgcaccgc cggggatcac agcaaatgta ataaccgtgc agaaaatgat
    54721 tcaaatctat catagtggat tattatcacg agcaaaagtg atgcttttat tcaccaatcc
    54781 ccaagatgtt gaagcggttg tccgctgcgg tgtacagttg aagtcgttga atatcggcgg
    54841 tatgagtttt actgaaggca aatcaatgat taccaatttt gtttcagtaa atcaacaaga
    54901 tattgattcc tttcgttact tgaacagtca agaagtggag ttagagattc gaaaagtacc
    54961 tgctgacagg aaaatccttt tgatggaact actagcgaaa atcaaaaagg tgtagcaaga
    55021 atggttgttg gaaaaggtca aataattctg ttgtaatggt tagcaattat taatattttt
    55081 gaatttgtta caacaaaagt aaataggagg tataaatatg gtagggatta tcctagcaag
    55141 tcatggacaa tttgctgaag gaatcttaca atccggttca atgatctttg gcgaacaaga
    55201 aaacgtcaaa gccgtgattt tgaaaccaag tgaaggtcca gatgacttga gagccaaatt
    55261 ggaagaagcg gttgcttctt ttgataatca agacgaagtg ttattcttag ttgatttatg
    55321 gggcggaaca ccgttcaatc aatcaaacac attattcgaa gagcacaaag ataaatgggc
    55381 aatcgtcagc ggtttgaatt taccaatgtt gattgaagcc tacgcatcac gcttttcaat
    55441 ggaatctgct catgaaattg cagcacacat cattgaaaca gcaaaagacg gtgtaaaagt
    55501 aaaaccggaa gaattagaac cggcagaagc accgaaagca gcagttgaag acgcacaacc
    55561 taaaggagca cttccagaag gaactgtggt cggagacgga aaaatcaaat atgtattagc
    55621 acgtgttgac tcacgtctgt tacatggaca agttgcgaca gcttggacaa aagcagtgca
    55681 accaaaccgt attatcgttg tttcagatgc agtatcaaag gatgatctgc gtaagagact
    55741 aatcgaacaa gctgctcctc caggtgtaaa agcaaatgtt attcctatta gcaaaatgat
    55801 cgaagtagca aaagaccctc gttttggaaa taccaaagca ttgcttttat ttgaaaatcc
    55861 tgaagacgta ttgacagctg ttgaaggtgg cgtagacatc aaagaattaa atgttgggtc
    55921 aatggcgcat tcggtcggta aagtcgttgt aagtaaagta ttgtctatgg gacaagaaga
    55981 cgttgaagcg tttgagaagc ttgaacaaaa aggtgtgaaa ttcgatgtac gtaaagtacc
    56041 taatgattca cgcgataaca tggatgatat tttgaaaaaa gcaaaagctg aattagcaaa
    56101 agcgtaataa ataatattaa aatttatagg aggcttatca tgtctattat ttcaataatt
    56161 ttagtcgtat tagttgcctt tctagctggt atggaaggta ttcttgatga atttcaattt
    56221 catcaaccac tagtggcatg tacattgatc ggtttagtta ccggtaacct cgaagctggt
    56281 atcgtacttg gcggtacgct tcagatgatc gctttaggtt gggcaaatat cggtgccgct
    56341 gtagcaccag atgctgcatt agcatcagta gcatcagcaa ttattttagt tttaggtgga
    56401 caaggtgtta aaggtgttcc ttctgcgatc gcgattgccg ttcctttggc tgttgcaggg
    56461 ttgttcttaa caatgatcgt acgtacaatc gctgttccaa tcgttcattt gatggatgcg
    56521 gctgctgaag aaggaaacat ccgtaaggtt gaaatgtggc acatcattgc tgtatgttta
    56581 caaggggttc gtatcgcggt cccagcagca gcgttgctat ttatcccagc acaaactgtg
    56641 caatcattcc ttgaatcaat gccagcatgg ttaacagatg gtatggctat cggtggggga
    56701 atggtcgtag ctgtaggtta cgctttggta atcaacatga tggctacaaa agaagtatgg
    56761 ccattcttcg ttattggttt cgttgtagct gcaatttctc aattaacatt gattgctcta
    56821 ggtgctttag gtgttgcatt agctcttatt tacttgaacc tttctaaaat gggtggttca
    56881 tcaaataatg gaggcggaag caactctggt gaccctttag gcgacatttt gaatgactat
    56941 taatcttgaa ggaggaaaat aaaatggcag aagaaaaaat caaattatca aaaaaagatc
    57001 gtttagctgt tgcgtggcgc tctaccttca ttcaaggttc atggaactac gaacgtatgc
    57061 aaaacggtgg ctgggcattt tctatgattc cagcaatcaa aaaattatac aaaacaaaag
    57121 aagaacgttc tgcagcatta aaacgtcact tggaattctt taatacacac ccatacattg
    57181 cttctccaat tttaggggtt acattggctc tggaagaaga acgtgcgaat ggcgcgcctg
    57241 ttgatgatgt agcgattcaa ggggttaaag taggtatgat gggtccttta gctggtgttg
    57301 gtgacccagt gttctggttt acagtacgtc caatgctagg tgcactaggt gcttcattag
    57361 ctatgggagg aaatatcctt ggaccaatca tcttcttctt aggttggaac attattcgtt
    57421 gggcattcat gtggtataca caagaatttg gctacaaagc tggttctaaa attactgatg
    57481 acctttctgg cggattgtta caagacgtaa caaaaggtgc ttcaatttta gggatgttcg
    57541 tattagctgc ccttgtacaa agatgggtat caatcaaatt cttaccagtt gtatcaactg
    57601 taaaattaga taaaggtgct tacatcgaat gggataaatt gccttcaggt ggtgaaggaa
    57661 tcaagagtgc atttgaacaa gttaataatg gcttagccct ttctcctgaa aaggtaacaa
    57721 cattacaaga caacttagac caattgattc ctggtttagc tgcattagga ttaacattgt
    57781 tatgtatgtg gttgcttaag aaaaaggttt ctccaatcgt tatcatcctt ggtttattcg
    57841 tagtcggtgt tgttggacac gtcatcggac ttttataatc cataaaaata tagctcgggc
    57901 gttgtcccga gcttttttta tatagtatac tgttcataaa caaatatctg gagggagaaa
    57961 atggttcaat caatcaatac aaaagtagac ttggtaattg atgctacttc gtttacgggt
    58021 ctgacagatt acgggcaaat catgataggt gataatggtt ttgagtttta ccatgcgcga
    58081 gatagacgca aatatatcca aattccatgg gaagaagtgg attatgtact agcatcagtc
    58141 atgctaaagg gaaaatggat tcctcgatat gccattcaaa cgaaaaaaaa tggtacatac
    58201 acattttctt ccaaagaacc taaaaaagtg cttcgtgcga tcagagaata tgtagatgaa
    58261 gatagaatgg tacaatcatt gagtttcttt gatgtagtga aacgtggtat tcgtagtatg
    58321 tttaaacgca agtaaaaaat aaagaaaact tctaagagga ggttttcttt atttttttac
    58381 tttgcagaag aaaatatgaa atagcaaaac caattatttg tcccctttaa atcccatatg
    58441 ctaaaattga ttagcaaaga aggaggttat attatgagtt ggttatggac attgattgtt
    58501 ggagccgtga tcggagcaat tgcaggtgcg atcaccagta aaggaaattc gatgggctgt
    58561 gtaacgaata tcattgctgg actagtcggt tcaagtattg gacaagcgat tttcggcgaa
    58621 tggggaccaa aagctgcagg tatggcactt attccatcta ttttaggcgc agtgattttg
    58681 gtagcagttg tctcattttt ctttggaaaa cgatcataag tttttacaaa atagtaaact
    58741 aaaaggagtt gcagcgagac tgtgactcct ttttggcttt atttatttaa aataaaaaga
    58801 agttttccgg aaatttttat ttaactgtcg tgtacgaaga aaagtttgct ataatggaaa
    58861 aaagcgtgca aagaaagagg gagtttcatt atgatgctac gtgtagaaaa attgcgcaaa
    58921 aaaatgcaag aagaaaattt ggattcattt ttggttacca gtccgtacaa tcttcgttac
    58981 ttaacgaact ttacaggtac gacaggatta gctgttatta ctttggaaaa agcatttttt
    59041 attactgatt ttcgttatac cgagcaggcg gcagcgcagg ctcaagggtt tgaaattatt
    59101 aaaaatgtcg gacctatttt tgatgaggta gcaaacttag tacgcaaaga agaactgcac
    59161 tcattagctt ttgaggaaac aacagtctca tttttagaat attcggtttt agaagaaatc
    59221 atcgatgcgg aattagtacc tgtttcacag atgatcgaag aactcagaga agtgaaagat
    59281 gaagaagaaa tcgctattat cgaaaaagca tgtcatattg cggatatggc atatgatcac
    59341 atcctgaaaa tgatccaacc gggcatgact gagattgaag tcgcaaatca actagacttt
    59401 tatatgcgtt ctcttggcgc ttcaggtgtt tcttttgata cgatcgttgc tagcggattg
    59461 cgttcggcta tgccgcatgg tgtagcaagc aaaaaaatca tcgaacaagg tgacttgatc
    59521 acattggact ttggttgcta ttacgaaggg tacgtttccg atatgacacg tacattcgct
    59581 attggtgatc ctggggaaaa attaaaagaa atttatcaaa tagtcttaga tgctcaatta
    59641 gcagttattg atgctgcaaa accaggaatg actggtgtcc aattagatgc agttgctaga
    59701 gattatattt caaaacatgg ttacggggaa gcatttggac attctacagg acatgggatc
    59761 ggtttagaga tccacgaagg accaaacgtt tcttctcgtg cagaaaagca atttgtacca
    59821 ggaaatgtga tcactgatga accagggatc tacttaccag gaatcggtgg agtacgtatt
    59881 gaggatgact tgttgatcac agaagatgga aatcgtgtgt tgactcgttc accaaaagaa
    59941 ttgatcatct tatagtttca tcaatagaag aagtaagcgt aaaaatcagt gttcacagac
    60001 tttgttatag aaaatcactc gatttaatga tacactatag aagaactttt aagctgtaag
    60061 agataataaa gttgggtgtt ttctccaatt catagcagtt tgcagctgat cggcggcagt
    60121 tttcttacca cgtgatcact atgccgcatc atagagaatt cggatgtatc tcgtgcttag
    60181 gcaaagggag gaataaataa atggcagatg aaaaaaattt gattttaaac gcaaaccaag
    60241 atttaggaga gatcgtgatc gctcctgaag tcatcgaagt aattatcggg attgcagctt
    60301 ctaaagtaga aggcgtatac ggaatgcgcg gaacttttgc cagcaatgtc actgaattat
    60361 taggacgtgc agctcacgga aaaggtgttt acttagcaaa cgaggaagat ggcttgaaag
    60421 tagatcttta ctgttatcta gaatatggtg tttcagttcc taaagtagca atggaaatgc
    60481 aggaacgggt cagccagcaa gtccttttca tgacggatat cgatttatct gaagtcaata
    60541 tccatgtagt agctgttgtt cctgaaaaat tagaacaacc aacaatcgaa gaactttttg
    60601 aagacgaaga ggaagagaat gaataaagaa ttatcaagac acgagattcg ggaaatggca
    60661 ttgcaggctt tatttccatt agacttcaat gcagacctaa caaaagaaga tgcgattttc
    60721 aacgcaatcg aattagacca ccgcgacatg atcaacgaag atgaatcaga gtttgttcct
    60781 gtttatcttg atacgctagt tggcggtgtc tgtgcaaaaa aagatgaatt ggataaagtg
    60841 attgaaaaac atttaaaaaa caattggcac atcaatcgga tctcaaaaat ggatttggtg
    60901 atcttgcgta tcgcgatttt tgaaatgact tatgtgacag atgtaccagc ggctgttgct
    60961 ttaaatgaag caatcgagtt ggcaaaaaca tttagtgatg accgttcacg taaattcgtc
    61021 aacggtgttt tatccaatgt attgaaagaa ttagaagctg gggcgtaagt cctagctttt
    61081 ttctatgaat catcataaac ctgttataaa tcgggaaaat tctatggtag aatgaaaaca
    61141 caaaaaatat ctgggagtgg ttagatggca gaattgatta acggaaaaga attagctgaa
    61201 aaaatgcagg cagagattgc tgaaaaaatc caaaaattga aagataatgg tatccatccc
    61261 ggattagtcg ttttattggt aggggaaaat ccagccagcc aaatctatgt aagaaataaa
    61321 gaacgtgtag caaaagaaat cggaatccat tcattggtag agcgttatcc tgagacgatt
    61381 tctgaagacg aactacttgc agaaattgaa aaatacaatc aagatcctag ttttcatggt
    61441 atattggtcc aattaccatt gccaaagcat atagatgaag aaaagatcct gttagctatc
    61501 cgccctgaaa aagacgtaga cggttttcat ccgatgaatt taggcagact ccttgcgggt
    61561 aaaccggaca agattccttg tactccatac ggcatcatga aaatgtttga agcttattct
    61621 atcgatccgg cgggtaaacg agcagtagtt atcggacgaa gcaatatcgt cggtaaaccg
    61681 atggctcagc tactcttgat ggcagatgca actgttactg ttgctcattc taaaaccgaa
    61741 aatctggctg aactggcaca agaagcagat attttagtcg tagcaatagg acgtggacat
    61801 tttgtcacaa aagaattcgt aaaaccaggt gctgtagtga tagatgtagg gatgaatcgt
    61861 gatgctaacg gtaagctgat cggtgatgtg aaatttgatg aagtggagcc tattgcaggc
    61921 tggatcactc ctgtgccaaa gggtgtaggg ccaatgacta tcacgatgct gatgtatcaa
    61981 accgtaaaat ctgccgaagc ttttggagca ggtgaataga tggcacaaga atatttaact
    62041 gtcactacat taacgaagta tttgaaaaga aaatttgatg ccgatcctta tttagaacgc
    62101 gtatatttaa caggcgaaat atcgaatttc cgtttacgtg ccaatgccca tcaatatttc
    62161 agcttaaaag atgaccatgc aaaaatatct gctattatgt tcaaaggagc attccaaaaa
    62221 ttacgattcc agccaaaaga aggaatgaaa gtccttgtcg ttgggcgtat ttcattatat
    62281 gaagcgggag gttcttatca aatctatgta gagcacatgg agcctgatgg aataggtgct
    62341 ttatatcaag aattggaaga acgaaaagaa aagctgatga aggaaggcct ttttcaaggg
    62401 cctaaaaaaa tgctgccacg tttcccaaag cgaatcgcgg tccttaccag tccaagcgga
    62461 gcggttatcc gcgatatcat cacgacggtg aaaagaagat atccaattgt ccaattggtt
    62521 ttgttcccaa cagttgtaca aggaaaccaa gcagcggaca atgttgtcag gaatatccag
    62581 agagtagaag agatgggcaa tttcgataca atgatcatcg gtcgtggggg tggttcgatt
    62641 gaggatttat ggccgtttaa tgaagagcgg gttgctcggg cgatttatca agctaatacg
    62701 ccggtgattt catctgtagg acatgaaaca gataccacga tcgctgattt agtagcagat
    62761 gttcgggctg ccactccaac agcagctgca gaattagctg ttcctgtttt gtcagaagag
    62821 ttgatgcgca ttcaagaacg gcaagctaga ttgcagcagg cgtttgtacg ccagatccag
    62881 cgcaaacaag aacggttcga gcgtgctcga cgttcctatg tatttcggca gcctgaaaga
    62941 ttgtatgaag gtcagaccat caaattagac cagttgaaac agcgattata tcaagctaca
    63001 cagcaaattt atcatgcgaa agaaaaacaa acgatcgcat taagccacca attggaacaa
    63061 gtggctccgc tttatcgagt taaagcagct cgccaagaaa ctgcttatct agaaaaacgc
    63121 ttagaagaga aaatccaaca atatatgaaa caacagcagc agcgttttgc tcaagctgtt
    63181 caatctcttg atttgttgag tccattgaaa atcatgggaa gaggctacag ctatacgacg
    63241 ttaaatgatc aagtagtaaa aagtgtcacc gagattcatc aaggggattt gctgacgatc
    63301 cattatcaag atggaatcgt taaaggacaa gtacaagaga tagacaaagg agaataaaaa
    63361 tatggaaaat ccaacttttg aagagtcact gcaagaatta gaaaaaattg tcatgcagtt
    63421 agaacaagga gacgttccat tagaatcagc tctagattcg tttaaacgag gaatggaatt
    63481 aagcaaacat tgccaagata cattagctaa agcagaagag accttgacga aaatgatgac
    63541 agaatcaaat gaagaagttg attttgacgg aaacgaggaa agctgagatg agattaactg
    63601 acttttcagc tattcatcta cctgctgtag aaaaagaaat cttgtctttt ttagatgagc
    63661 atacaacgga tagaggactc tatgatgcga tgagttattc agtgaaagca ggcgggaaaa
    63721 gaatacgtcc cttattactt ttaacggccg ttgcttcctt tgatgagcct attgatgttc
    63781 ccgtctatca agtagcagct gctttagaaa tggtgcatac gtattccttg atccacgatg
    63841 atttgcctgc gatggacaat gatgatcttc gtagaggaaa acctaccaat cataaagtat
    63901 tcggcgaagc attagctata ttggccggag atggtttgct tacaggagca ttccagctaa
    63961 tcagtatggc acacttaggt aattctccta agcttctgct gcttcagcaa ttagctgttt
    64021 gtgcgggatc acaaggtatg gttgctgggc aagctgcaga tatcgagggc gaatcgaaaa
    64081 agctttcttt agaagaatta gcatttattc atgaaagaaa gacagggcat ttgattcgtt
    64141 atgccttgct ggctggtggc atattagcga agcaacctga agaaatctta ttgctacttc
    64201 aacgattagc cgaacattta ggtttagctt ttcagataag agatgatcta ctcgatgtga
    64261 ttggtacaac gaaaacatta gggaaaacag ctggtaaaga tgaacgtatg gaaaaaaata
    64321 cttacccgcg tttattaggc ctagaaaaga cacgtgaggc gttggagatt gagctgttat
    64381 ccgccaacaa gatcatagac aaattagaag aggacgtttt ttcttttgat ggcgggctga
    64441 tgcgccagat gatcaagcaa tttgatgtgg agaaatgagg gaaagaatgg aaaaagaacg
    64501 cgtagatgta ttagcggtca aacaaggatt atttgaaaca agagaacaag ccaaacgttc
    64561 agtgatggct ggtttgattt acaacgaaaa aaacgagcgg tttgataaac cgggagaaaa
    64621 aattccagtg agtagtgaat taagagtcaa aggtaaaaaa cttccttatg tttctcgagg
    64681 gggattaaaa ttagaaaaag cactcaaagc atttgatcta tctgttgaag gaaaaacatt
    64741 gttagatatt ggtgcatcca ctggcggatt tactgatgca gctttgcaaa atggcgcaaa
    64801 aatgagttat gctttagacg ttggctacaa tcagctagct tggaaaataa gacaagatcc
    64861 tagagtcgtc gtaatggaaa gagtgaattt tcgttatgca aaacccgaag actttacctt
    64921 aggaattcca gaagttgctg ttatcgatgt ttcttttatt tcactaaaac tgatgctgcc
    64981 tccattacat gctattttaa aagaagaggg agaagtgatt gctttaatca aaccccaatt
    65041 cgaagcaggc agagaagctg tagggaaaaa cggaattgtt cgtgaccctc agacccatca
    65101 aaaagtttta gaagacattt tgtcatttgc ggcacaaggt ggatatgatg tgctggaatt
    65161 aagttattct ccgatcacgg ggggagaagg aaatatcgag ttcttggctc acttgaggaa
    65221 agtacccgaa agcggaacaa tcaattcagc tattaatatg gcagaagtgg tttcaaatgc
    65281 acacgaacag tttgaccata aataaattgt aactgtgaaa atcaagtgag aagtatgcgg
    65341 attcttcttt attataccaa ggaaataagc tatcattgat taagaagaat atgctaagga
    65401 gcgaagacgt aatgagaaaa aaagatcggc atcgtttgat tactcgattg ttgagtgagc
    65461 aagatatccg aaagcaggaa gaatttgttg aaatcctgaa aagcaaagga atatctgtta
    65521 ctcaagctac gatttccaga gatataaagg aactgaaact gatcaaggta ccggcttcag
    65581 atggcggcta ccgttatagc ataccagctg aaacaaacga agatgtaaat actaaattgg
    65641 aaaaactatt aaaagacgct ttcgtggcag ttgaccaaat ggaaaaattt gttattttga
    65701 aaacattgcc gggaaatgct tcggcagcag caaatttgat tgataaacgt tacaagaaag
    65761 aattattttc gattatcaat gatgacgata atgttttgat gattacacga actgaagacc
    65821 aggcagtttt attgaagaaa gacttcctgc attaccttta agttgaggag cgaatgtgat
    65881 gctacaagaa atcagtatta ctaactttgc gattattccg gaattgcggc tttcttttca
    65941 cgaaggaatg accgcgctga ccggagagac tggtgcgggt aaatccatta ttatcgatgc
    66001 tttaggtctg ttagcaggag gaagaggttc aagcgattat atacgtcaag gagcagaaaa
    66061 atgtgtgctt gaagggttgt ttgaactacc aaaacaagaa ggattctctg aactgatggt
    66121 tgaattaggg atcgaaacag atgaggacaa tctgattgtt cgacgggata tgtccttaac
    66181 tggaaaaaat gtttgtcgag tgaatggtca tattatcaca ttagctaatc tgagaagaat
    66241 cggcagttat ttagtagata tccaaggaca aaacgaacat caggaactgc tccagcctga
    66301 atcacatctt gctttattgg atcgttttgg agatgtagct ttccaacaga agaaaaagag
    66361 ttatcagcaa gaatatatct cttatcgtga gctggaaaaa agggtacgaa aagtgcagca
    66421 aaatgaaaaa tcctatgttc agagaataga tatgctccat ttccagcaag aagaaatagc
    66481 tgctgctgat ttgcaagtcg gagaagaaga gaaattaaaa gaagaaagag aaaaacttag
    66541 caattatcaa aaaattgctg atggattagc tgcaggttac ggagcactga cgaataatga
    66601 acaaaacagt gtagatggtg ttggcttggc cgtttcagaa ctgcaaggga tcgcccatct
    66661 agatgtggag tatgaagcaa tttatgaaaa tatccaaagt gcttactatt tgctgcaaga
    66721 tgcaatcggc gatatgagtc gtcagattga tttattggag ttagatgaga gtcgtttaga
    66781 agaagttacg caacgtttag aattaatccg acagttgaaa cgtaaatacg gcgaatccat
    66841 tgagtcaata ttggcttact atgacgaaat caccgaagag ctagcttctt ctgatttttc
    66901 agaaggtcaa ttggacaaaa tgaaagagga actagagcaa aaagagcttc tacttcagca
    66961 acaagccgca gatcttcatg aagcgcgtaa aaaaatcgca aaagaattgg aaaaatccat
    67021 tctgcatgaa ttgaaaagtc tgtatatgga aaacactgag tttgaggtac gtttcttaaa
    67081 agaagaaaat agacaattga atcatgatgg atttgaccaa atcgaatttt acattacgac
    67141 caatccagga gagccactga agcctttggt gaaagtcgct tctggtggcg aactgtctcg
    67201 gatgttgctt gctttgaaaa ctattttttc ttctgagcaa ggtgtgacaa gtattatttt
    67261 tgatgaagtg gatacaggtg tgagcggaag agttgcacag gccatcgcag ataaaatttc
    67321 tcaaatatcg aaatactctc aagttttgtg catcactcat ctgcctcaag ttgctgcagt
    67381 cgccgactat caatattata ttgtgaaagc agtgattgat ggacgtacgc aaacttctgt
    67441 ttcagagttg aaaacaaaag aaagagaaga agaaattgca cgtatgctgg ctggaagtga
    67501 gattacaaaa ctcacactcg aacatgcaaa agaactattg aaactcgcaa aaaggacctg
    67561 agccacctcg cagtggttca ggtccttttc agcagctgat agctatattt tatttttaca
    67621 tgacactcat tacagcagtc aatacaacag atccaatcgt taaaatcagc attgtccaaa
    67681 tgacaaattt tgttacttta gcaaaagtac ttgtttgttt tttttcacgc attgttttca
    67741 acctcgtcat taattagttg attcaagtgt acaatctttt ttccatttcc acaagagggc
    67801 ttttttataa aaacttccct ttttttaaat aataggtagt tacgatacaa ataaataatg
    67861 ttaatagaac tagccatata aaggcatcat ccctttcagc aataggtaac ttaatgttca
    67921 ttccatagat actgccgacg attgtcggta tagttaaaat aatcgttaaa gaagttaaaa
    67981 ttttcataat attattcaga ttattcgata cgattgctga aaaagtctga ttcatttgtt
    68041 ctactagttt taattgaatg cgagaagtag tcattgcctg ctctgtttcc accaatacat
    68101 cacgtaaatg atttttcaaa gcatgatttt cttggaaaag tttattttgc gctagttctt
    68161 tcaatgcatc taaattagca gaagtcgctt ctctgaaata aacgaggctt ttttggatat
    68221 ccatgatttg ataaagttgt ttgttttcag tggatacttg aagtttggct tccaaagtat
    68281 cgcattgaga ttgaagcgta gctaaataac tgttgtaaga gagtgtgacc tgccaaatga
    68341 attgaaagaa gatattgaga ttaggcgaaa tatcactttt tggaaaggtc tggctaaaga
    68401 ctttctttaa aaacagtggt tcatgattcg taattgtgat aagcttcttt ttgggcgtca
    68461 aaataataga gagcgggtat gtatccactt gaaaataacc gctaggactt gttttagcaa
    68521 aaggatattg taacaataat aacgctggct ttgtcaactt tgattgattg aattcttctg
    68581 tacgggaatt ttctgcatcg tccaatatgg aagtgatgta atctgacgga atttcaaatt
    68641 gatgtgtcag ctgatgaatc tcttcttgat taggacgttc tacacaatac cagatcgttt
    68701 ggtcagtttc ctttccggta ttacgcagtt tctcattatc taatgtaaaa tatttgatca
    68761 tgattttcct cctgttgcct gtttctttta ttataacgga tagggaattg gaatacatgg
    68821 aatgtgtttg taagatatag aatataactg cataaattta aataaaatga aacagttgtg
    68881 taaagtatat acagtatact tgaagcaacg ataaaaaagg agtttacgtt catgggcaca
    68941 ttcacaccac ttatcttgtt ttggtatatt tacccggtaa tcgttttgtt tggttgccaa
    69001 tttcttgtaa aattattttc gttaaatcgt cgttttaaaa taaaagcccc tgatttggcg
    69061 attccttttt tgtggggcgg tatgcatgca ttgtctagaa atacagggac aatttctatc
    69121 ttaccgtttt tctttatttc tgttttgcta atgggaattt gtattgctgt tttccaagca
    69181 tattattatg aagaaattat ttatccgcgt tatttcaaga tgacttggcg tttggttttt
    69241 ctgttgacaa tcgtcttgta cgctgtcttg atcattgtga acatactttc ttacctatga
    69301 aaaaaataga aaagctgtaa aaagtctttt aaaaggcttt ttgcagcttg atttttaaaa
    69361 aaatgtttat gaataaaaaa agttgttccc ggatttccca gaaaaggtgg tagaaagtgg
    69421 gggattgtgg tagactaatt ttagttagtg gaggaatggg gggctttcaa tgttgatggg
    69481 tgaattccaa cataatattg atgcaaaagg acgtttaatc gtgccctcaa aacttcgtga
    69541 agaacttggc gaaaagtttg ttctgacacg cggattggat ggctgtcttt tcggctatcc
    69601 gatgagcgaa tgggaaaatc tggaagctaa actgaacgaa atgccgttgg ctaaaaaaga
    69661 cgcacgtact tttgtgcgat tcttctattc ggctgccact gaatgcgaac ttgataaaca
    69721 aggacgaatc aatataccca gcacgttaag aaattatgca gctttgacaa aaggttgcgt
    69781 agtcatcggt gtatctaacc gaattgaaat ttgggatgag acacgatggc aagatttttc
    69841 tgcagcagca gaagagaact ttgacgaaat tgctgaaagt atgattgatt tcggactata
    69901 agaataggaa gaggctgact aatgactgaa acatttcaac actatacagt catgttgaaa
    69961 gaaacggtag atggattgaa catcaaaccg gatggcgttt atgtagactg tacactcggc
    70021 ggtgccggac atagtgaata tttattaagc caactaagtt ctaaagggca tctgtatgcg
    70081 tttgaccaag atcaaaaagc aatcgatcac gccaagctgc gtttagcttc ttatgtcgaa
    70141 caaggtatgg tcacatttat caaagcgaac ttcagagaat tagaagaacg cttgagtgag
    70201 catgtagata aggtagacgg cattttgtat gatctagggg tttcctctcc ccagctagat
    70261 gaagcagaac gtggatttag ttatcatcaa gatgcaccgt tggatatgcg catggatcag
    70321 tcagctcctt tatccgctta tcacgtaatt aatgaatatt cctaccatga attagtaaag
    70381 attttcttcc gctatggcga ggagaaattt tccaaacaaa ttgctcgtga aatcgaacga
    70441 gtacgaaaaa caaaaccaat ccaaacaact ggtgagcttg tcgaaatcat taaacaagtc
    70501 atcccagctc ccgcaagaag aaaaggcggg catcctgcaa aaagaatttt tcaagctgtg
    70561 cggattgcag taaatgacga actcggtgcc gaagaggcct ctttagaaca ggcaatccgg
    70621 ttattgaaag tcaatggaag aatcagcgta atcacattcc attcattgga agacagaatt
    70681 gtcaaaagta tgtttaaaga atatagtacg gtacaagatc taccaccggg acttcctgtc
    70741 atacctgacg aattccaacc agaactgaaa gtcattactc gtaaaccaat ccttccttca
    70801 gaggaagagc taacagaaaa caatcgatca cgcagtgcaa agttacgtat tgcagaaaaa
    70861 ataagaatga aagaggagta gaatgatggc agaattaaag aaaatgcaag attacgaata
    70921 tgatttacct gaattggtgg atcagccgga acaacaaatg cctgattctt cttcgaatca
    70981 agaaatcatt cttccacaat caccggcaaa acgattaaag cagatttctg ctttggaaaa
    71041 aattgctgtg gcaagcgtgg cgattgctat tgttgcttta tgtgttttaa cagttatgct
    71101 gcgcacaaat atcagcggag ctgaaaaaga aatcacgtca atacaaacag aaatcaatga
    71161 taaaaagcaa gaaaaaacga gtttagaaca agaaaaaaac gaactttctc gtactgaacg
    71221 gattaaacaa atcgcagaag aaaaaggatt gtctattaat gacgacaatt taaggaaagt
    71281 gaagtaaatg agtcttaaaa ataaaattcg gcggtttatg gaaaagaaaa atctaaatcc
    71341 gatgaacaat cgcaaaaaag taggtatcat tttatttgct acgagtattg gattgttctt
    71401 tttatttgcc gtccgtttct cgtatatcgt tattggaggt catgttgctg gtacctcatt
    71461 agctgaaaaa acaaaacagt tgtaccaagg aagtgaagta gtaaaagcaa aaagaggaac
    71521 gatttatgat cgtaacggtg tggcgctcgc agaagatgcc tcctcttatt caattaaagc
    71581 gatactctca aaaacatata cctctgggga taaaaaactg tatgttgaag aaaagaattt
    71641 tgataaaata gcagaaatcc tgcataagaa tctttctata gacaaaaaag atgcactgaa
    71701 tattttagag gacggcgcaa aaaaagagct gtatcaagtt gaatttggta gctatgggaa
    71761 aaatatctcc caagaaacca agcaaaacat cgaagcggac atgaaaaaag aaggtgtcgc
    71821 tggtctttat tttgtcgacc atcaggctcg gatgtatcca aatggcgtct tttcttctca
    71881 ttttattgga tacgcagttc cagacaaaga tgagaatggc ttagttggta aactaggttt
    71941 ggagtctgct tacaatgata tcttgagcgg taaagatggt aagattattt atcaaaaaga
    72001 taattttcaa aatcctctgc caggtacagt ggcagaagaa gaaaaagctg tcgacggaca
    72061 agatatctac acgactttag acagcagact acaaagttat ttagaaacac tgatggatca
    72121 agtaaatgaa gaatatcagc cagaagaatt gacagcagtc ctgatgaaag caaaaaccgg
    72181 agaaatcttg gcaatgggac aacgacctac tttcaatcct gaaacaatgg aaggattgac
    72241 tggaaaagat gctatctggc gtaatttctt ggtacaagat agttatgaac caggatcaac
    72301 catgaaagtt ttcactactg cagctgctat cgaagaagga gaattcaacg agaatgaaac
    72361 cttccaatct ggaaaaatcc aagtagaaga tgctacgatc aatgaccatg actttggtga
    72421 aaaaggtgtg ctgaccatgc gtcaagctct ttcatggtca agtaacgtcg ggatggtcat
    72481 actggaacag cgattaggcg gacgatggta taactattta caaaaactgg gatttggaca
    72541 aagtactcat tctggattag atgatgaagt aaacggtgct ttacctacat taaatattgt
    72601 cgaccgagca atgagtgctt atggacaagc ggtaggtgtg acgaatttcc aaatgatgaa
    72661 agctttcact tctattgcga ataacggtac aatgattcaa cctcgatata tcagcaaagt
    72721 ggtggatccc caaactggag aagagcgaac aactcaaaca gaagttctag ggcaaccttt
    72781 ttcaaaagaa acaacagaaa aagtccgtga atatatgaga gatgttgtcg aaagcgaaaa
    72841 ctacggtatt gcttatggtg tatatagtgt acctggatac aatgtaacgg caaaaaccgg
    72901 aacagcccaa atcgcttctg atacaggggg atatcaaaca ggagatacag cctacctgta
    72961 ttcgattgtg gagatggttc cttctgaaga tccagactat gtcttgtatc taacaatgaa
    73021 acaccctaag acatatgatc gaatggcatt ggctaaaatc gcaaatccat tgatgaaacg
    73081 ggcaatggat tttaaagaaa ctgaagaaga ttcagataca gaaacaaaaa cagaaaaagt
    73141 atccgtagcc gattatcgta atcttgaagc ggacgttgca gcagctgatg ctcaaaaaag
    73201 tggactgcaa cctgtagtga tcggtaatgg taaaaaagta caaaaacaat ctactgccaa
    73261 cggtgatcag ttgatctctg gagaaaaact gatcctatat actggtggtg ataagctgat
    73321 gcctgacgta acaggatggt caaaagcaga tatcatgaag ctggggaaaa tcctcggtat
    73381 tgaagttagt tttgatggag atggttattg tgtcaaacaa gaacttgctc catatgaaaa
    73441 aatcactgaa gacaaactta gttttacttt agaagaataa gaagagaaat aggagagaaa
    73501 agagcatgga atggacacaa gcattgattc ccattgtcag cagttgtgca atgacaattg
    73561 ccgcaatgcc gctatttatt ggttattttc aaatgaaaaa gcaaggtcag gcgattcgtg
    73621 aggagggacc aaagtggcac aatagcaaag ccggaactcc tacaatgggc ggcttagtgt
    73681 ttctgatcgg ctctattttg acaggaattt gggttggagc ttggcaaaaa cagctaactc
    73741 ctactctttt catcttgctt tttgtattag cgttatacgg agtaatcggc tttcttgatg
    73801 attttataaa aatattcaag aaaagaaaca tgggactgaa ctcgaagcag aaattgattg
    73861 gacagattct agggggaatc atcttttatc ttgtttaccg ttctgaaggt tatccaggtg
    73921 tattgaattt cttcggacta gatctacctt taggaatcat ttatggtatt ttcgctctct
    73981 tctggcttgt tggattttca aatgcagtaa atctgacaga tggcatcgat ggattagttg
    74041 ctggcttagg aagtatctct tttgcagctt acggtctaat tgcttggcat cagcatcagt
    74101 atgatgtatt aatcatttgc ttaagtgttt taggaggttt actgggtttc ttcgtctaca
    74161 acagaaaacc tgccaagatt tttatgggag atgtcggttc attagcgttg ggcggacttt
    74221 tagcggcaat ttctataatg ctaaaccaag aatggacatt gcttttgatt ggtttgatct
    74281 atgttatgga aacagcaagt gtgatgcttc aagttacttc ctttaagctt acaggaaaac
    74341 gaatttttaa aatgtctcca attcatcatc actttgaaat gtgcggatgg tcggagtgga
    74401 agattgatac agttttttgg ttgatcagca ttgtgacttc gttgatcaca ttatggatcg
    74461 tttggtagga gatgacggga aatgaaaaaa atatcaacat atgaaaataa gaaagtgctt
    74521 gtcttaggac tcgcaaaaag cggagtaagt gcggccaaat tattacatga attgggcgca
    74581 cttgtcaccg ttaatgatgg aaaacctttt gacgaaaatc cggaagcaca agagctgctt
    74641 tctttaggga taaaagtaat aacaggtagt catccaatcg aattgttgga tgaagaattt
    74701 tcattgatgg tcaaaaatcc agggattccc tacagtcatc ctttagtagc aaaagcccaa
    74761 gaaatgggta ttccggtaat tacggaagtt gagctagctt acgaagtagc ggaatgccca
    74821 attattggga ttacaggaac gaacggaaaa acaacgacaa caacgatgac aggactacta
    74881 ctgaatgcag gagcagatca aggaattgca cgtttagcgg gtaatattgg gtatccagca
    74941 agtggtgtag cgcaagaagc aaaatcagaa gataaaattg tgatggaatt gtctagcttc
    75001 caactcatgg gaatcacaga tttccgccct catatagcgg tgattaccaa tatctatgaa
    75061 gcacatattg attatcatgg tacaagaaaa gaatatgtca aagctaaatg gaaccttcaa
    75121 aaaaacatga cagaaaaaga ttatctgatc cttaactgga atcagtcaga actacaagaa
    75181 ttagcgcaaa gaacaaaagc acgcgtactg cctttctcga caaaagaagt gttggaagac
    75241 ggtgtgtatg ctgacgatta ctcaatatac tataaaaaag aaaaaataat ggaaatatct
    75301 gaacttggcg taccaggtaa gcataatgta gaaaatgcac tagctgctat ttctgtagca
    75361 aaattgtatg gtatatcaaa tgaagctata cgagaaacgt tgcatttttt ccatggcgta
    75421 ccacatcgta cgcaatatgt aggagaaatc caaggcagaa aattttataa tgattcaaaa
    75481 gcaaccaata ttttagcgac taaaatggct ctaagtggat ttgaaacgtc taaggtcgtg
    75541 ttattggctg gcggattaga cagaggaaat acatttgatg aattaattcc ttcattaaaa
    75601 ggaatcaaag caatggtcgt atttggccaa acaaaagaaa agttgatgga tgctggaaaa
    75661 aaagcaggca tcgagacaat agtaacagcc gattctgtag aacaagcagt tcctttagca
    75721 ttagaaaaca gtacagatgg tgatgtagtt ttattatcac cagcgaatgc cagctgggat
    75781 caatacccaa attttgaaac tagaggaaac agattcatgg aagctgtgaa ccgattaaaa
    75841 tagaaaaagt agaagcgagc gataaaaaat gaaaattttg gttacaggcg gaggaacagg
    75901 gggacatatt tatccagctc ttgcctttgt caattatgta aagactcaag aaccgaatgc
    75961 tgaattcatg tatgtgggag caaaacgagg tctagaaaac aaaatcgtcc ctgatacagg
    76021 aatgcctttc cataccttgg agattcaagg atttaaacga aaaatttcca tgcataacat
    76081 caagacggtc cgattgtttc ttaagagtat ccgtcaagca aaaaaaatcc tcagtgattt
    76141 tcaaccagat atcgttatcg gtactggagg atatgtttcc ggagcggtag tgtacgctgc
    76201 agccaaaatg ggtattccaa cagttatcca tgaacaaaac agtgtaccag gtgttaccaa
    76261 taaattttta agccgatatg tggatcggat cgctctttca tttaaagatg cggcttcctt
    76321 ttttcctgaa aacaaagcag ttcttgtagg taatcctcga gcacaagaag tagcagatac
    76381 taaaaaatca gatattttga aaacatttga tttggatcca gaaaagaaaa cagtcttgat
    76441 ttttggcggt agtcaaggag cacttaaaat caatcaagca gtatcagttt ttttaagtga
    76501 atatattcct tcttcttacc aagttttgta tgcttcagga gatagatatt atgaagagat
    76561 cctaaataaa ataaatcctg aaatacaaaa tattagtgtc cagccttata tcaaaaatat
    76621 ggcagaggtc atggcttcca gtgatctgct ggtggggcgt gctggtgcaa cttcgatcgc
    76681 agaattcaca gctctggggt taccggcagt gttggttccc agcccatatg ttacgaatga
    76741 tcatcagact aaaaatgcaa tgagtcttgt tcacgcagga gcagcaaaaa tgattgcaga
    76801 taatgaacta acaggtgaat cactaagtca gacagtcaac gaaatcatgg gtgatgaaga
    76861 attgcaaaag caaatgtgcc gagcttctaa agaacaagga atccctgatg cttcaaaacg
    76921 tctttatgac ttagtaaaac aaataatcaa aaaataaagg cggtggtctt agtgagtaag
    76981 aaaaaagaac caaaaaaatt gtctcatccg tcttctgatg aaaacaattt gactccttgg
    77041 caaaaggcta accgaaaata tctggcagaa caagagatga aaaaccaaga gccaaaaaaa
    77101 gaagacataa cattagatac acccctttca gattctgaag aagaaaaaac ggttcaagag
    77161 gaacagcaag aagaaagcca gtccgagaaa aaaatcgaat tttttgaaga gatcaagcaa
    77221 aagaaaaacg gtggaccata taacggttct tttttgaacc ggctgcccaa tttaaaaagt
    77281 caacggcaca aagtactatt tcgtcggtta gctttgatca tcactgtatt gggtataccg
    77341 ctgatttttt tgatctatta tgtttctcct tatagcaaat tacaagcagt tgcagtatca
    77401 ggcaataaga cagtcaacag ccaagaaatc atctctgata caaaattgtc attaggagaa
    77461 aatgtttggg gacagtattt tcatcgatct acttatatcg atcgccttaa aaaagcacag
    77521 ccaagaatcg aaacagcaaa tatacatttc aaagggatga atgaattcga tttggatgta
    77581 acagaatata aagagattgc tttgatcgca aaaaacaatc aatactaccc agtgattgaa
    77641 aatggaacag tattagatga aaaagtagca aatcctacaa aaaatctccc tatcttggaa
    77701 gagttcaaag actctgcaaa aatcaaagaa ttaacaaagc aatacaatca gttatcatca
    77761 gaattacaaa aagcaatttc tgaaatcaag tacacgccaa gagcaagcaa taaaaatctg
    77821 atccaattga atatgaatga cggcaatcaa gttattgtaa atatcaataa tcttgcgaac
    77881 caaatgaaat attattcgca agtagccaaa gatatggatg aaaaaggtgt gatcgacatg
    77941 gaagttggta tcttctcgca tccttacggt agcactcaag aaacggaaga atcgggccaa
    78001 tcggaagaaa caactgaaag tgaagaaaat gcggatcaaa caaacgaatc aagtgaagag
    78061 gcagaaacgc aaaaagcaca aacacctgga tcgtctagca caaacggatc atctgattca
    78121 aatgattcaa gtgaaacaag tcaaatagaa gataatccaa gtacaactcc gtcaagttta
    78181 aatgaaaatt gataaaacaa ctttaaaaag taagaatgta ctttctaaaa accagtaatt
    78241 tatggtagaa ttggaagcag tgaatatatc tattaataag tatattttaa aacggacgct
    78301 agttgttcgc ttgtatatgg aatagtaagt aggagggaat cccattcatg gcaaaaacag
    78361 gaatgtatgt aggccttgat attggaacga catctgttaa agttgtcgtg gctgaatata
    78421 tcgacagcca aatgaatatt attggagtag gaaacgcaaa atcagaaggg attaaccgag
    78481 gcattatcgt tgatatcgat aaaacggtcc aagcgataca acgagcagta cgacaagcag
    78541 aagaaaaagc aggtatccag atcaaaggcg taagtgtcgg tttgccagca aacatgcttg
    78601 aagtagaaaa ttgccaggga atgattgcag ttaacggaga ttcaaaggaa atcaccgatg
    78661 aagatgtcag aaatgtggct tcagctgccc ttgttcgttc aatacctcct gaacgtcaaa
    78721 tcgtgtcaat attgccacaa gattttactg tcgatgggtt cgagggaatc aaagatcctc
    78781 gtggaatgat cggcgtaaga ttagaaatgt acggtctgtt gtttacagga cctaaaacga
    78841 tcatccataa catacgtaaa tgcgtggaaa atgccggatt aatcgtcaac gaaatggtga
    78901 ttactccttt agcgctgact gaatcaattc tttctgacgg agaaaaagat tttggtacga
    78961 tcgtcattga tatgggtggc ggccaaacaa caacggctgt tatgcatgat aagcaattga
    79021 agtttaccaa tcttgaccaa gaaggtggcg aatttgtcac taaagatatc tctattgtat
    79081 taaatacgtc atttaacaat gcagaagcgt tgaaaataaa ctacggggat gcatacccag
    79141 aacggacatc accagatgaa gaatttccag tagatgtgat tggtcagtca gaaccagtga
    79201 aagtcgatga acgctacttg tcagaaatta tttcagctcg aatggaacag attttcaaca
    79261 aagcaaaaga tgcattggac caaatcgaag cattagagtt gccaggtggc gtcgtattga
    79321 caggcggagc tgctagtctt cctggtgttg tggacttggc acaagaaatc ttcggcgtaa
    79381 atgtaaaatt gtatgttcca aatcacatgg ggctgagaaa cccggtattt acaaatgtaa
    79441 taagtatcgt ggattattca gcaaacttga gtgaagtgta tcaattggca aaaattgctg
    79501 ttacgggtga aacctcagca gctcgtcaaa tggtagttga acaagaaaca acaaacacat
    79561 atgagagcta tgaagcacct gaagaaacaa tctacgatga gccggaacca aaagagtccg
    79621 gagaaaatgt aaaaaataaa atcaagggtt tctttacaaa tatttttgat taattcatcc
    79681 tgggaggaat aagatatgga attctcaatt gataataata tcaatgacgg cgcagtaatc
    79741 aaagtcatcg gagtcggtgg tggcggtgga aatgctgtaa accgaatgat tgaagaaaac
    79801 gttaaaggtg ttgaattcat tacagccaac acagacgtgc aagcattaaa aaattcaaaa
    79861 gctgaaacag ttatccaatt aggccccaaa tatactcgtg gtttaggtgc tggttctcag
    79921 ccagaagttg gacaaaaagc ggcagaagaa agtgaacaat ccttacgcga agctttagac
    79981 ggtgcagata tgattttcat cactgcagga atgggtggag gaaccggtac aggtgctgca
    80041 ccaatcgttg caggtatcgc gaaagaattg ggcgcattga ctgttggtgt cgtgacacgt
    80101 ccattcactt ttgaaggacc gaaacgtggc cgttttgctg cagaaggtat cgctcgtttg
    80161 aaagaaaatg tggatacatt attaattatc tcaaataacc gtttattaga agttgtagat
    80221 aaaaagacac cgatgcttga agctttccgt gaagcagata atgttttacg acaaggtgta
    80281 caaggtatct ctgatttgat cacagcgcca ggttacgtta accttgactt tgctgacgtg
    80341 aaaacagtga tggaaaacca aggtacagca ttgatgggga tcggtgtggc tagtggagaa
    80401 gatcgcgtag tagaagcaac gaaaaaagca atctcctctc cattgcttga aacatctatt
    80461 gatggagctg aacaagtact tcttaacatc acaggcggtt tggatatgac attgttcgaa
    80521 gcacaagatg cttcagatat cgtagcaaat gcagcaactg gcgatgtaaa tatcattcta
    80581 ggtacatcta tcaatgaaga aatgggcgat gaaatccgtg tgacggtcat cgcaacaggt
    80641 atcgatgaat caaaaaagga acgcaagtct agccgaccag ctagacaagc acaaatgcaa
    80701 tcacctgctc aaaagactgt attagatatg gatcaagcta aaccaacttc ttcagaagaa
    80761 gaaaacagtt ttggcgattg ggatatcaga cgtgaacaaa atgttcgtcc tagagttgat
    80821 gattccaatt ttgacaatat cgaaaaaaaa gaatttgata cattcaaccg cgaagaaaca
    80881 aaatctcatg gcgatgatga attaaatacg ccaccgtttt tccgtcgtaa aaaataaagg
    80941 agaggaagag cacatgattg ctgacaactt gcagaaaatc aagcaagaaa ttcaggattc
    81001 gtgtgctctt gttcatcgtg atccgaaaga agttacatta gtagctgtaa caaaatctgt
    81061 tgaatccgct caagctgaag aattggttag agctggcgtt atgaattgtg cagaaaaccg
    81121 agtagataaa ttattagaaa agaaacaaga tttatttgat tttcctgaaa taaagtggca
    81181 tttaattggt aatttacagc gcagaaaggt aaaattgatt gtaaatgaaa ttgattactt
    81241 tcatgcgttg gacagtttaa gattagcaga agaaatccag aagcggttag agaaagaatt
    81301 atcctgtttt attgaagtaa atgtttctgg tgaggaatca aaacatggaa tttcaccaga
    81361 tgagctttta ccgtttgtgg aatctttagc tgcctgcaca aaaatcaagg ttgttgggtt
    81421 gatgactatg gcgcctaaag aagcttctga tgagcaaatc cataaagtgt ttggtacgct
    81481 gaaagaattg cgtgatcttg ttcaagcaaa aggttatgcc catgcaccat gtagtgaatt
    81541 aagtatgggg atgagtcaag actttcaaat tgcgattgaa gaaggtgcaa catttatccg
    81601 tgtaggaaca gcgctattca aagacgaata aaggaggaac aaaaatgccg cttttaaaca
    81661 gaggagcaat tgctaatttt ttcggattag ctgatgagga agagtacgaa taccaagatt
    81721 atcctgaaac aacaaaaaag gctgtacagc caactaaaaa aaatacaaca cagaatcaat
    81781 caactcattc tgtatctgct gcgaatactc atgaaaaaca aaaaacggcg acttcacgtc
    81841 ctgtaacgca tgaaagtatt cagccggctg aaaagaaaaa agtaattgaa ctacatcaat
    81901 cacctacaaa aactgtagcg aaagaaagaa cagcttcaag aacctctaca caacctaaag
    81961 caaatcttgc tggaaaaatc actgtaatgg aaccaagagc ttattctgaa gctatggcga
    82021 ttgctaaacg aatcattgct ggagaagcaa ccttagtgaa ttttcattta gtagaagaaa
    82081 aacaagcgag aagaatcgtt gattttctaa caggaacagt ttatgcacta gatggagata
    82141 tcaaacgtgt tggagatgag atttttcttt gtacaccgac tgggatggag attgacagtt
    82201 caaccgctca atcttttgca gatgcgaatt tgtttgatct gtaagaggag gaactacgat
    82261 ttatactatt gcactattac ttcatttact tcaacgggca gcctatttct atagcattat
    82321 cttgatggta tatgcattgc tctcttggtt tccaggcggg tatcaatcag ggctaggacg
    82381 attattagct aagatctgcg aaccgtatat cagtttgttc gatcgtctgc ctttgaggat
    82441 tggacccttg gatttcacaa ttttaggggc aatcctgatc ttgaacttag ccgtacaagg
    82501 tttagcacag atcgttatat ggataactgt ccatttagtt tattgattga agcaacaaaa
    82561 tctttattct cagaatagat aggaggggaa aattttggat gcaaatgtat accagcactt
    82621 tcgggcagat gaacgtacat ttatcgattc ggtaggcgat tggattgaac aggttcaagg
    82681 acaatatgca ccctatctga ccgaatttct tgacccaaga caatcctata ttttagaaac
    82741 actgattcga caaagcagtg atttgcgttt catgttttat ggcggatatg aacaggcaga
    82801 aagaaaaaga tgtttgattt tccccgatta ttacgaacct gtagaggaag attttgaaac
    82861 agcattgtat gaaatccatt atcctagcaa atttgccagc ctaagtcatg ggaaaatcct
    82921 tggaacttta gtgggaacgg ggatcaaacg ctcttacttt ggcgatatca tctcggacgg
    82981 tgatcgctgg caagtattta ttgcaaatga ggtggatcat tttgttgctg cacaagtaac
    83041 aaagattgga aaggttgctg tacgcttgga accaatcaaa tacacagaaa tgattatgcc
    83101 gaaagatagc tggacacaag aaagaggaac agttagttcc atgcgactag acagtgtgat
    83161 ctctgagatt tataatattt ccagacagcg gtccaagcag ctgatcgaag ctggtaaagt
    83221 aaaagtgaac tggacagaaa atacaagacc tgattttgta cttgaactat tagatattat
    83281 ttcagtcaga ggttatggca gagtccagat ccaagaactt gaggggaaga cgaaaaaaga
    83341 aaagtacagg gtactgttcg gtgtattacg taaataaaat aaaaaggtgg tttactcatg
    83401 gctttaactc cattagatat ccaaaataag acattcccga caaaaatgcg gggatataat
    83461 caagacgaag tagatgattt cttagattta gttgttcgtg actatgagga attgactcaa
    83521 agaaatcgag aattagaaaa agcagtcaaa cattctgaag aaaaacttga gtacttcaat
    83581 gaattaaaag atgctttgaa ccaatcaatc atcgtagctc aagatacggc tgataaagta
    83641 aaaacaagtg caagcaagga atccgaagtg atcgttactt ctgcacaaaa caaagcagat
    83701 gagttagttg cgaacgctga aaaacgtgca catcaattaa caactgatgc agaagaaaaa
    83761 gcaagaaaaa tcttgacaga tgcaacagaa aaagcaagac agcttgcgac agaaactgaa
    83821 gatcttaaga aaaaaacacg agtattccat caacgtatca gcttgatgct tgagtctcaa
    83881 ttagaacaag tgaaaagtcc ggaatgggat gaaattttac aaccattctc cagctacgtg
    83941 actgacagcc atgaagtgat caaagaagtt ttagcaaaac agcttgacaa tgaaaatgat
    84001 actgatgtaa actcagacac aggagtcata gaagcaccta tgacggcatt tgatacacag
    84061 gctattgata tctcgatcgt gcctggtgaa aatgaaaacg agtagaacag aaaagcactt
    84121 tgttattctt aaagcgagcc gatgagagtg gaagttcggt aagacactgc atggtgaaag
    84181 atcctctact tgagacctgc ttttccgaaa ttagagtaag aagcagcggc tgacctcgtt
    84241 attggtcaca gagagtttgc actagataga tgcaactaaa tttgggtggt accacggcat
    84301 gttcgtccct tgcggctgaa catgcttttt tgtgtccgga aaacaaataa aggagtgtat
    84361 ggttgaatga aaatgaaaga aaccttgcaa ttaggaaaaa caaaattccc tatgcgtggt
    84421 aatttgccaa accgagaagg cgagtggcaa aaagaatggg aagaaaatcg aatctaccaa
    84481 aaaagacaag agttgaacga aggaaaacct agctttgttc ttcatgatgg acctccatat
    84541 gccaatggta atatccattt aggacattca ttgaataaaa tcagtaaaga cattatcatt
    84601 cgttcaaaat caatgtctgg ctatcgtgca ccatatgttc caggatggga tacacatgga
    84661 ttgccgattg aacaagtatt ggctaataaa ggcgtgaaac gtaaagaaat gtccatggca
    84721 gaatacttgg aaaaatgtcg tgaatatgca ctttcccaag tagataagca acgagcagat
    84781 ttcaaacgcc taggagttgc aggagattgg gaacatcctt atgttacatt agatccttct
    84841 tatgaagctg cacaagtacg cgtatttggt aaaatggctg aaaaaagcta tatttacaaa
    84901 ggattgaaac caatttactg gtctccgtca agtgagtctt ctttagcgga agctgaaatt
    84961 gaatataaag atgtaaaatc tccatctatt tttgtagctt tcaatgtcaa agacggcaaa
    85021 gatattcttg atgaagatac agcttttgtc atttggacga caacaccatg gacattgcca
    85081 gcaaaccaag gtattgctgt aaatccaaaa tttacgtatg tattggtgga agccgatggc
    85141 cgtaagtttg tcgtagcgaa agatttgcta gaaacagtcc aatcagaaat cggttgggaa
    85201 aatgtaacag ttctaaaaga atttgctgga caagaaatgg aatatatgac tgcaagtcat
    85261 ccattctatg aacgtgaatc acttgtgatt ttaggcgatc atgttacttt agacgctggt
    85321 actggattag tccatactgc tccaggacat ggggaagatg actatatcgc tggtagcaaa
    85381 tataaactat ctgtagtgtc acctgtagac tcaaaaggat tgtttacaga agaagcacct
    85441 ggatttgaag gtatctttta tgacaaagca aatcctatga tcactgatct attaaaagaa
    85501 aaaggagctt tgctgaagct agatttcttt actcatagtt atcctcacga ttggcggacg
    85561 aaaaagcctg tgatctatcg ggcaactcca caatggtttg catctatcga taaattccgt
    85621 cagaatattt tggatgaagt agaaaaagta gagtgggtta tcccatgggg taaaacacgt
    85681 ttgtataata tgatccgtga ccgcggcgat tgggtaattt cccgtcagcg tgcatgggga
    85741 gtaccacttc cgattttcta tgctgaaaat ggagaagcaa tcatcacacc agaaacaatc
    85801 gaacatgtgg caaaattatt tgctgaacat ggatcaatta tctggtttga aaaagaagcg
    85861 aaagaacttc ttcctgaagg atttacccac ccaggttctc ctaatggcga atttacaaaa
    85921 gaaaaagata tcatggatgt ctggtttgat tctggttctt ctcatgaagc tgttttacgt
    85981 cagcgaccag aattatcatt cccagcagat atgtatttag aaggatcaga ccaatatcgt
    86041 ggctggttca actcaagtat cacgactagt gtggctatca atggtgtagc accatacaaa
    86101 tcagttattt ctcaaggatt tgctttagat ggtgaaggcc gtaagatgag taaatcttta
    86161 ggaaacatca ttgcaccaga aaaagtgatc aaacaatttg gtgcagatat ccttcgctta
    86221 tgggtatcta gtgtagatta tgaagcagat gttcgtgttt ctatggatat cttaagtcaa
    86281 gtggcagaag tatatcgtaa aatcagaaat acgatgcgtt tcttattagc gaatacagaa
    86341 gatttcgatc ctaaaaaaga tactgtttcg tacaatgatt tacgttctgt agacaaatat
    86401 atgaccgttc gattgaacca agtaatcaaa gaaatccgag aagaagggta cgacaaatat
    86461 aacttcatgc atatttaccg tacagttatg aacttcttga cggttgatct atcgtcattc
    86521 tacctggatt ttgctaaaga tgtagtttat attgaagcag aagatgatta tcaacgtcgt
    86581 tgcatgcaaa ctgttttcta tcaaacagct gtagctttga caaaattatt gactcctatc
    86641 attccgcata cagcagaaga aatctggtca ttcctgaaag aagaagagga gtacgttcag
    86701 ttaagcgagt ttcctggtta tgaagagttt gcaaaccaag aagaattgat ggatacctgg
    86761 actgctttca tggacttccg tgataatgtc ttaaaagcat tggaagaagc aagaaacagc
    86821 aaattgattg gtaaatcttt ggaagcaaaa ctaactgttt atccaaaaca acaagttcgt
    86881 gaaatgttaa aagctttgga tgcagatatt gcacaattat tgatcgtttc accagactac
    86941 tttgaagtta tggcagcaga tgaacaagtg ccagataacg ctatggtatt tgatgatgtt
    87001 gctattacag tggaaaaagc agacggggaa acttgcgatc gctgtcgcca agttcgtaaa
    87061 gatgtaggag tagatgaaaa acttcctcat ctatgcggtc gttgtgcaaa aatcgttgaa
    87121 gacttctatc ctgaagctgt agctgaagga ttcgaagaaa agtaaaaggg aaaagggggt
    87181 gtgacatgtt ctttgtcaca cccccttttg ttatcaatcg tttaattttc cacgttcacg
    87241 ataccagtta tctggttgcc agatccattc gaatccgtct ttttcaagta gttcgaaagc
    87301 tgctttaggt cccattgatc cagctttgta gttgggaaaa tccggttgtg tttggtccca
    87361 agcttttcga atgacatcca cgatatgcca agattgagcg acttcttccc aatgagagaa
    87421 gttcgttcca tcaccattca acgcatctaa tagtaatttt tcgtaagctt ctgggctatt
    87481 ttcaaccatt tctgcactat ttctaaattc caatttgatt ggttccgttt caaatccttg
    87541 ccctacttct ttcccattca aggataaaga aaatccttca gttggctgaa tatagatcgt
    87601 tagaacatta ggtggaagtg tcgtatcgtc acatggttca tcgacagatg ttttgaatac
    87661 attaacagga acttgtttga atacgatgtt gatccgagtc cctttttcag tcaaacgttt
    87721 gcctgtacgt acatagaaag gaacacctga ccaacggaag ttatcgatag tgaatttgcc
    87781 tgcgacgaat gtttcagtct ctgattgagc atccactttg tcttcttcgc gatagccctt
    87841 gaattgtaca ccatccaatt caccttgagc atattgcccg cgaacgaagt tttctaacac
    87901 ttcttcttcg ttataaatgc ggatagcttt caatgctttg atcttttctg tacggatttc
    87961 tttttcggaa aatgctactg gcggttccat tgcaagtaat gctacaacct gtaaaatatg
    88021 attttgcacc atgtctttaa gtgcgccact atgatcgtag tagcccccgc gatcttctac
    88081 accgaggttt tcgccaaaag taatttggat attgtcgata tagcgattgt tccattgagc
    88141 ctcaaaaata ttattggcaa aacggatagc tgagatgttt tggatcattt cttttcctaa
    88201 ataatgatca atcctgaaaa tatcttgttc tgggaatact tctctgattt gctggttcaa
    88261 ttcaaaagca gactggtaat cagaaccgaa cggtttttct atgatcaaac gattgaagcc
    88321 attttttgtc ataatatttt gtgatttcaa atgagaaacg atcgttccaa aaaattgagg
    88381 tgccatagcc aaataataga cacggttacc gtttaattga tagtgtccat ctaatcggtc
    88441 agacaattct tttaatgtct gataatgcgt tgaatcattt acatcatggg attgatagta
    88501 aaaatggctt gcaaaggtag ttgcttcttc atctgaagga ttcaatgcac ggattgtttc
    88561 tttgacaact tcacggtagt gctcatcgct ccagggacgg cgagcggttc cgattacagc
    88621 aaaatgctcg cctaaatttc cttttctgta aaggcggaac aaagacgggt acaatttacg
    88681 ctgggctagg tcacctgtac cgccgaatat cgtaaacaat acattttttt cattagacat
    88741 gtggatacac tcctttatat tttatcgaca aaaatcgtgc tggccgcctt aaagctgacg
    88801 gggattttct ttctatcatt ttggatagtg atcggtcctt cataagctgc gatgtcaacg
    88861 atctcatatt cttcgttgat attcaggtca atagtgacta agtaatccaa caattctttt
    88921 tcatcaagca ctcgtgcgat acgcactttt gtaccgacag gataggactc caatgtttgt
    88981 cggcgttctt catggacaac ttcctctttt ttaggaatcg atcctccatg aggacaatgt
    89041 tcaggatgat tcaaatagtt ttctaaatga tctgcaagat gttccgaagt cacatgttcc
    89101 aatacttcag catcatcatg gacttcattc cagctatagt ctaaatgttc aactaaaaaa
    89161 acttcccata agcgatgttt gcgaatcaga ctgctagctt tttgcagtcc gcttgcagtc
    89221 aattgtacac cttgataagg ggaatgttct acaagctctt ctttcacaag tttagaaatc
    89281 atttcactga cagatgcagc tgagacatct agactagata cgatttgttt attgttgact
    89341 ttcgttgtat ctccacctag ttctaaaatg attttcaaat aatcttctcg atttggtgtc
    89401 atattcgatc atcctttcat ggtaaaaaac catcattagt aagtgtaaca aaaaaaacgt
    89461 taattgacta tttttctaaa taaaaaacaa aggattttta gacgttttta caacaaaaaa
    89521 cgaaagatga tagagaagac tcattctttc gtttataaag gatatatata tacattggga
    89581 gggatctatt tttcagataa caacttatat gaagtgtctg cagattcgtc ataaagagtt
    89641 tgttcgggat aagaaaagat aagataaaaa ccatactgat cacgtacgac taaacgatat
    89701 tcatctattt ctaccacaga tgtgccaaga gactgaccgt tgatcaaaaa cgtgtaatca
    89761 gatgtaatct gtaattgttg tttttgctgg ctatcactaa agaaccatgt accttgatac
    89821 tttgagagtc cttctgactt ttccgcttgt tttttctttt tcaagtaaat aaaacttgag
    89881 agaacggtaa tgagtgaacc aagaaaatag atcatttttt ttatcaccaa atcactcctt
    89941 tatgagaaaa taatcgcata gacatattct ttcttcttta tttcattgta ccgaataaaa
    90001 gctaaaattt ctctaagact ttcgactggt ttttttactg tttttctaca aattatgata
    90061 ggatacatag taggaagaaa ggtgacataa tttgaagtat cagcaagtgg aaatagcttt
    90121 ttttattgac cgcccgaatc ggtttatagc acattgtatg aaaaaagacg gagaaataat
    90181 tattgcgcat gtcaaaaata caggtagagg aaaagaggtc ctgataccag gcgctgaagt
    90241 ggctattgta tttgctcctg gacctaaacg gaaaaccgcc tatgatctga ttgctgtaaa
    90301 aaagaagggt gactggttta atatcgatag tcaattgcct aatcggctgg caattgatgg
    90361 tattttagac ggcacgatcc ttttaccagg attaactggt ccatttacgg tatacaagcg
    90421 tgaagtaact tatcttcact ccaaatttga tatttattta gagacggtgg tcggacaaaa
    90481 agcatttgtt gaagtcaaag gtatgacact tgaaaataaa gcgattggtg cttttcctga
    90541 tgcgcccaca ctcagaggat tgaaacatat aggagaatta acgtatgccg cacaagatgg
    90601 ttatgccgct tatgtcttgt ttattgctca gttcgaacat cttcatctgg caacgatcca
    90661 tgaagaaatg caaccagcac tagctgatat ggttcgacat gcgcagcaaa gcggagttca
    90721 aatcctcgcc tataattgtc aagtatcggc agatgaggta actgtcaagc aggccattcc
    90781 atttgatgta gatatccctt ttgaagatcc tatacaatag aaagaaggta aaataatgat
    90841 caacttaggt attatcggaa caaattggat tactcatcaa tttgtccaag cagctttaag
    90901 cacaaaaaaa tatgaattga cagcggtata ttctagacat ttggaaaaag cacaggaatt
    90961 tggttctcat tatgaagggg atatcgaatt cgcaacagat ttgaagactt ttttcaatat
    91021 tactcatatg gacaccattt atattgcttc tccgaatagt cttcacttta gtcaagctaa
    91081 gcaagcaatt ttggcaggaa aaaatgtgat agttgaaaag ccagcttttt cgacgccaaa
    91141 agaaatggat gaaatcatcc atttggcaaa tgaacacaat gtgtattttt ttgaagcagc
    91201 aagaaatatc catgaatcag gttttaaaaa gattgccgat tacttaccat taaaagacga
    91261 gataatcggt gcaaatttca gctatatgaa atattcttcg cgctatgacc aagtattaga
    91321 tggagaagaa ccgaatatct tctcgactca tttctcaggt ggtgcattga tggatttagg
    91381 tgtttacctt gtatatgcag ctgttgcttg gtttggaatg ccaaaagaag tccattattt
    91441 cccaagaaag atttctactg gtgtagatgg tcttggatgg gggatcttac gttatgagaa
    91501 ttttgatgta tcgatccagc caggtaaaat cggtgattct tatttaccgt cagagattta
    91561 ttttgactca ggaacgctag tgatgaatgg agtcaatgca attgaacaag cacaatttca
    91621 tgatcgcgcg cataaagaaa tcattgattt ggcggtaaca gctgcagaaa atccaatggc
    91681 agatgaagca gaggattttg ccaatgtcat ggctcatccg aaagatccag cttggggatt
    91741 gttgtatgaa gagtggatcg agctagctag aaatgtgaac caagtgattt atgatttaag
    91801 aaaaaatggc ggcatcaaat tcgatgcaga taatgaggaa gatttttaat gaacgaattt
    91861 gtacaagtat ttcccgaaaa ttggcagaaa aaatatgagg ataaagggtt tatctcacct
    91921 tctcttattc aacgagcagt ttttcagcca ctaagtaata aacaaagtat agtagcggtt
    91981 tctcctacag gttcaggtaa aacactggct tatctttggc ctctactact aaacgtggaa
    92041 ccaggtgaag caagtgcgtt ggtcattttt gcttcttctc aagaactagc tattcaagta
    92101 gcagatgttg cacgtgaatg gggaaaagac aaagagttga aagtccaatc tttggtgggc
    92161 ggagccaacg tcaaacgtca gattgagggt ttgaaggaaa aaccagaaat cttgattggt
    92221 acaccaggaa gaatattgga attgatgaaa gcaaaaaaaa taaaagcaca tcaagtgaaa
    92281 acaatggtat ttgatgaagc agaccagcta tttgacgctg ggaacagcca aatcattgat
    92341 caaattcttc atcaagctcc aacagagtat cagctagctt ttttttcagc gacagcagat
    92401 cgttcccttg aaagcattga aaaaatcact gggaaaacgt ttgagacaat tgacgtgaca
    92461 gcagaggacg attctcgaaa aggactgaga cattactttt tacgtgtacc gatacgaaag
    92521 aaagatgact acttgagacg tctcacgcat atagaagatt ttcatggtct gattttcttt
    92581 aatcaactga ctgaattagg cgtaatggaa gaaaaattgc tgtatagagg tgtaccggta
    92641 ggaagtctgg cctctgatca gaacaagcta ctccgaaaag cagcgctaga acaattcaaa
    92701 aagaaaatgg tcagagcact tttgaccaca gatgtggcgg ctagaggttt agatattaca
    92761 ggtcttcctt atgtagtgaa tgcagaagtt ccgttaagtg aagaagctta tttgcatcgt
    92821 tcgggaagaa ctggtcgaat gggtaatgaa ggaatagtaa ttacactcgt acaagataat
    92881 aatctaagag atttaaaacg gcttttacgt ccatcaaata taacacttga agagattttt
    92941 ttatacggag gtaagctgca aactgagcag ccaaccaaag aagatgcagc tcctgcagta
    93001 aataaaaagt atgcgaaaac tttttctgga aaggagcaga acccagaata taacaaagaa
    93061 aaaataagaa aaaaagttaa aaataaatca aaaaaagaga gaaataaagg agcaaggcgc
    93121 aaataagcgc ctttttatgc tatgattatg aaaacgggaa caaaattggt tgccagagga
    93181 ggggttatgt gaaagaacgt caagaagaga tcttgcagct attgacagaa aaccaacaag
    93241 gattgacagc aagtgatgta gctgagcgct taactatcga tcgaagcaat gcaagccgat
    93301 atttaagtga actatacaag gcccatcata ttgtaaaaac cgctggccga ccagtcgttt
    93361 actctttgcc aacggaaaag tcaaaatctg atgaagtgca tgtcgactct tctacgcaag
    93421 tgacttttga aacactcgtt ggagaaaatg attctttaaa agtcagcata cagcaggcaa
    93481 aagctgcgat tctttatcca ccgcgggggt tgcatacgat tatttttgga gaaactggta
    93541 cagggaagtc gatgtttgct gaatgtatgt atcattttgc aatcgattca gagatgcttt
    93601 ctgcagatgc gccatttgtt tctttcaatt gtgcggatta tgctcaaaat ccgcaactgc
    93661 tctttggaca catctttggg atcaaaaaag gggcttacac aggagcagct caagatagtc
    93721 ccggtctaat agccaaagca gacggcggta tattgttttt agatgagatc caccgtttac
    93781 cgccggaagg gcaggaaatg ctgtttactt tcattgataa aggtatttac cgtccgcttg
    93841 gagaaagtgc acaagtacat gaagcatctg ttcagatcat tggggcgacg actgaatctt
    93901 cagaaagttt cttatctacg ttcaatcgac ggattcctat ggcaatcacc ttgccgaatc
    93961 ttgcttcacg ttcactggat gagcgttatg aaatcatctc tctttttatc aaacaagaag
    94021 cgaatcgttt gaatcaacgt atcgatatcg aaaaagaagc gattcttgcc ttcatgcttt
    94081 atgatgcgga aggaaatatc ggtcaagtga aacgagactt gaaactagtc tgtgcaaaat
    94141 cttttttaca ttaccgtact catcatgaga aaaagctaat cattcgaaaa gaagaacttt
    94201 cccttcaagt acaaaaagga ttactgaaaa tcaaggaaat accggaacgt ctcgaccgat
    94261 tcatggacag caaaagtcag tatttgacat ttgaaccagg atttgctgat gttgtctggt
    94321 cacaagatcc agaacgaaac atgcaggtct ataacgatat tgaggaaaaa gtcacttctc
    94381 tttccgaaac cggtgtggag aacattgatc tagagaccct catttcaaaa gatgtggatg
    94441 cttatttcca gacatatgtc aaagaattga ctaaaagttc gattcccaaa gatcttttgc
    94501 cagatgaaat ctggcggtta accaatcaat tgtatgatgt ggctgaaaaa cggttagacc
    94561 gtatttataa tgaaaaagct cgttttgctt ttgctttgca tctgcaaagt acattggatc
    94621 gtgtgagaga aggtcatatg atcgtccacc cagatttgaa taacgtacgg aaaaatttta
    94681 agagggaatt ccaggtagca atcgatcttt caacgatcat tgaagaagaa gaacaagttg
    94741 agattccttt tgatgaaatc ggctttatta gtatgtttct ttctatcaat ctaggagaaa
    94801 gtgagcctgt taaagaaaac aaagtagatg ttctcatatt gatgcacggc agacagactg
    94861 cttcaagcat gttggagatg gcacaagaac tattaggaac aaaagtaggg aaagctttta
    94921 atatgccact ggttatggaa gttcaaacaa tgtatgaaga agtgttacaa tatgtcaaaa
    94981 actatcaaga gcatttgacg aatggcctac tcttgttaac agatatggga tcattaaaca
    95041 ccttcagtga tttgatttat gaagagactg gcattcggac gaaagctatc acgatgacaa
    95101 gtaccatgat tgtgatagaa gcgatacgga tggcaagtgt tggaagaagc ttagaagata
    95161 tttatcaaaa tatccagctt tcctttgaat cgatcgtacg tgatcagttc aaagcggaaa
    95221 agtctgaaac tttacagcgt aaaaaagccg taatcgtgac atgctttact ggtgaaggag
    95281 tagctgccaa attatatcag cgtattatgc cagtgattaa tcaaaataag gtagaactga
    95341 tccagatgca gtttttagaa cgtgaatcat tcaaaaaaca catcgatgcc ttattggaag
    95401 aatatgagat caaagcaatt gcgggaacag ttgatgttga ttatcaaaat atcccatttt
    95461 tttcagccta tgacgttttt gatgatgaaa aactaaatat tttaaagcgt atagctgatg
    95521 atgagatccc gacagctaaa attgtcgatt cacttgctgg atcattaagc gatgtctttt
    95581 ctattgatga actgatccgt tcattgcaaa agacagttca gcagatccag accgatttgt
    95641 cgattatcgt ggaacctgga gtagatactg gaatcgttat ccatttagcc ttcatgttag
    95701 acggattgat caaacaagaa agccatcggc aatttgagaa actgtctatt ttctcaaaaa
    95761 tttatcgttt ggaaatggat atcgttcggg caaacttgat ggcactagaa cgaaaatatc
    95821 aggtaaaaat tccagaagat gatatcgcat ttcttacaca aatgtttctt gaaaataaaa
    95881 taaaaaaatg aacacactaa tcatactgtt tacacactaa acatctgtgt gcgcactata
    95941 aaaaagagct aaaaagcatg aaaacgctat ttcagctctt ttttttattg gcatgcttct
    96001 tgcgttatat aaaagtgtaa aaaaataaaa ggagtgttaa aaatggcaaa aaaaacaatc
    96061 atgttagttt gttcagcagg gatgagtacg agtttattgg tcacaaaaat gcaaaaagca
    96121 gcagaagaaa aagggatgga agccgatatt tttgcggtct ctgcctcaga tgcagataat
    96181 aatttagaat ctaagaatgt cgatgtatta ttattaggtc ctcaagtacg tttcatgaaa
    96241 gctcaatttg aacaaaaact tgcgcctaaa ggtattccat tagatgtcat caatatgcaa
    96301 gactatggca tgatgaacgg agaaaaagtt cttgcacaag cagaaaatct aatgaaataa
    96361 atcaagtatg cagttggggg tataacaacg tggaagatca acaaaatcta gaagccatca
    96421 tggggttaat catgtttggc ggtaatgcaa agagcgatgc aatggaagcg attgcggctg
    96481 ctaaaatcgg tgattttgag ttagcggata ggaaaatcgc tgatgcagaa gaatcactta
    96541 tacaagcgca tcatgcacaa acagacatgt tgacacaaga agcacaagga aatcatatgc
    96601 aagtgacatt attgactgtt cacagtcaag atcacttaat gacatctatt gcgtttactg
    96661 atttagcaaa agaaatcatc gaactttatc gtcgcgtata cagcgaataa atacaacttc
    96721 ttttttgaac ggaagaagaa aaatttttga acggattttt tattttgtta taaggagaac
    96781 tctctttata taaaaaactt ataggaggaa gtaaaaaatg aacggactat caagttggat
    96841 ggagaaatac atcctgccgg ttgccgcaaa aatcggagcg caaaaacatt tagttgcttt
    96901 gcgtgatgct tttatcggca cactgcccgc aacaatggcg ggatcagtcg ctgtaatgat
    96961 caatgcaatt attcgtgatt taccgcaaca gtttaacagt acctatgctg acagtttagc
    97021 agcagacaaa ggaatctttg ccgtattgaa tgtcatcatc ggaatcaatg gttttgtctg
    97081 gaacggtaca ctagcaatcg ccggattgat ctttgccttt tcttgggggt ataatcttgc
    97141 ccgagcttat ggagtaaatg atctagctgg ggggatcgtt ggtttagcta ccttgattca
    97201 aggtatttcc tttggtccag cattatcagc agcattagat gtgaatgttc cttcaaatgt
    97261 cgcagatgca atcaatagtt ctgagattgg tgcaacgatt gcagatggca cactgtcagt
    97321 tggattatgg ggttggctaa aactagatca tttgaatgga aacgcttatt ttacagttat
    97381 gatcatggga gctatttcag tcattatttt ttgtaaattg atgcttgcca acattacgat
    97441 caaaatgcct gattcagtac caccagcagt atctaaagca tttgcagcca ttttaccagc
    97501 gacgatcgct ttatacgtca ttgctattat caattatatc gtaggtcgtg tagctgatgg
    97561 gcaactgatc attgatttgg tgcaaaaata tattgcagaa ccattcctag gattatctca
    97621 agggttggga gcagtattga tcgtcacagt atttgttcaa gtattctggt tctttggtat
    97681 tcatggatca aatgtattgg caccagtgct agagggaatc tggggacaag cacaactaat
    97741 caatattgat attttccaaa aaggttataa cggaaaaact ggtacaccag ccgtgttaga
    97801 tgcaatcgaa gatggaaaag cttatatgtg ggtccgcgga tcgttcgatg cttttgcatg
    97861 gtttggcgga tcaggcggaa cgatcgtctt actgatagct atcttgattt tctctaaacg
    97921 tgcagattat ctaacagtag ctaaattatc cattgggcct ggtatcttca atatcaacga
    97981 accagtgatg tttggtctgc caatcgtatt gaatgcaatc atgtttatcc cattcttgat
    98041 tgctccagta gtagcaacag caattggatg gacagctact tatcttggcc tagtcgctcc
    98101 ggtatcgcag caagtcacat gggtagtacc gccgttctta ttgtcattct tagcaacagg
    98161 agcggattgg cgggcaccga tcgtaacatt agtatgtatg gtcgtgactt tcttgatttg
    98221 ggcaccattt gtcatctctg caaacaaaat ggaccctgcc gcaggcgaac aacaataaga
    98281 tgaacgtgag gagcgggacg ctttaagttt cgttcctcat aatgaatttt cgaggaggaa
    98341 ctgaaatgat tacaattgcc agtgcaatgc aacaagatga aatcaaagaa ttgttagaag
    98401 gatacaatga agaaggaatg acttttacat ttaaagaaaa acaaggaatc aagctgttct
    98461 ttgaaacaag tgcagaagat ccggaagttg cggctaaaaa agcaaaggaa ctaatcaaag
    98521 cacagccttg gggaacagtc ctttattttc aatcaacagc tgtttagaaa atggtttaaa
    98581 aagataggat ggttgagatg ataaaaaacg gtttattttt atgtagtatt ggttctttga
    98641 tcgtgttgta tagtttgaat ccacattcgc aaaggtatga tttgtatagt atggtcacag
    98701 gtctttttct aacaggatta ggaatctatt tcttcatgaa ggggaaaaaa agagaagaag
    98761 caaaggagaa aaataactaa tggaaatcac gaaaaaatta aatattccag catcgttttt
    98821 ttacgataaa gttatggact ctgtcctttt cgatgtacgt aaagcgaccg gcaaatcagt
    98881 aactagaaaa cagctgaaaa atttggagta cgtcaaacaa ttctcgcaaa ataaccgggc
    98941 tcgcataaaa atcgaagaag tcattgaaaa ccagtcttat aagtttcgta catcgacaac
    99001 gaagaatgaa ttcgtcgttc attatcaaat caaaccattg gacgattcga cctgtgaggt
    99061 tcattacaca gaaaaaatgg agtcttatgg catgcttcaa aagttgaatg atatgctttt
    99121 aggaacaatc ttgatgtatt ttaaacggag acgtttcaaa aaaatgctag atatgatcga
    99181 agcaacgtat taaatcaatg aaacatggta ctatttagtt attgaaaaat gaagtaagct
    99241 agaaggaatt tttcatagga agatagatct ttcctgcgaa tcctcactag cttttttgta
    99301 ggaaggggca gcaaaatgac aaacaaagta agagagctag gtgtatcttc gattgaggaa
    99361 atgtttgatt tagccactta tgcatttaat ggggcagata cacctgaaag gcgagaacgt
    99421 tttcgtacac ttgcggagaa ttcttggaat tacggatttt ttgatgagga ggatcggcta
    99481 acaagtcaag tcatggcgac cccttttcca gtgaattttt acggacaaga gtacctaatg
    99541 gccggcattg gctatgttgc ttcttatcca gaagcaaggg gacaaggcgg gatcaatcag
    99601 atcatggaaa agatccttga agattgccgt gatcgtaaag taagcttatc ttatctggcg
    99661 ccattctcct atcccttcta tcggcgctat ggctatgaac aatcatttga taaaatcagt
    99721 tatcaactaa acagtcgtga tgtgccgttt atcaaaaaaa aatctggaaa aataaagcgt
    99781 atggattttg aagatgcaaa agctgcaatc agaagaattt atgaacagat gccggaaaac
    99841 caacgagctg gtctgaagcg cgaagactgg tggtatacgt ataaattcaa gcaaaaaggc
    99901 aattaccagt ttgctttgca ttttgatgat aatgaggaag caacgggata tatcgtttat
    99961 cagcttgcaa cgtcttcctt tattattgcg gagtggggat ttttggatca tgatgctttt
   100021 tgcagccttg ttcgcttcgt aagttctcac aatggtgctt ttgaaacatt tgaatattca
   100081 tgcggctacg gcggtaatga tcaaaattat ctattagaaa atccatttgc ttctgtaatg
   100141 atcacacctt atatgatggc gaggatcgta gatatttctt tgtttttgga aaattatcca
   100201 tttaaaaaga gacaagctgc atttgcccta caaatagaag aagatcatta tgcgaaatgg
   100261 aatgaaggaa tcgtggaagt aacaatcgaa aatggaaaaa atcatctctc aaaagtagaa
   100321 caaacaaaac tcccaatcgt tcgaggaagt atccaacata tcacgcagct attattaggg
   100381 tatcgtaaag tggaagagtt ggtattccaa gaaagaatac aggtgacaaa agaatatgaa
   100441 gaaacgttaa gctcaatctt gccacagcaa cagccaattc ttaatgacta tttttgataa
   100501 aaaacgggtg atgacaatat ttttgtcacc acccttttgt ttttcatttg gcatttgaag
   100561 gaaaatgtta aagtaaatcc tcttttttcg taaatttgaa ttttttgttt tcttttgttt
   100621 tagggttttc ttttttgtgg tcttggattg cttctttcgt aatcttttcc cgattttttt
   100681 tagatgaatc taagcgttct gcgtatactt tttcttttgg cttatcattc attggaaagg
   100741 acctccctac attatttcat tctacctcta gtttatcatc tgttaaggac tagtggaaac
   100801 gttttgccta acttataagc tgattttttt agcagataag cttgcaagta aaaaacaaac
   100861 aagctacaat tgatttattg aaattgttcc tgtagctcag taggatagag caatcgcctc
   100921 ctaagcgata ggtcgctggt tcaattccag tcaggaacgt ataaataacc cctgtatcct
   100981 ttgcggtaca ggggtttgtt ttgtatttgt gccaaaaatg tgccaaacta gttatcattt
   101041 ttatataaac tatcgaacac agtattgatt ttttctttat cctgatcttc aagctctcga
   101101 acaatatgaa gataagtaga cattgtagtt tctaaactgc tatgtcctaa acgtttcgat
   101161 acgctaagta tatttactcc ttgataaagt aaaatagatg catgtgtatg ccttagtcca
   101221 tgcaaagtaa attgcttttc aagccctaat tcttttaatt ttttgcgcaa atatttgctg
   101281 acggcgtttg atgaaactaa tccatttttt aagttgaaaa aaacaaaatt atgagggttt
   101341 ttgatttcaa aattctcgaa taattctttt tgattgattt taaacttttt taagagatca
   101401 atcgtatgtt tatcaatgga aattttccta tttgaagttt cgttttttgt ttttccccat
   101461 tcatttaatt tataattcca tgttttatta atagtgatga tttgatcttc gaaatcgata
   101521 tcttcccatg ttaatcctag aagttcagca aatcgcattc cagtggcacc agcaactaac
   101581 acgagcatag gagaagagta ctgcgcagaa aggttttctt ctacgagttt cattaaagat
   101641 ttaaattgat cataatctaa atatttatct tcttttggct tcaatgaatc ttttcctttg
   101701 attactgctt ttctagttgg atcaaacgga attaatcctt cttctacggc atctttcaac
   101761 gacgctctaa tatggttatt aaacttaata acggtagatt ttacatggtc tttagcatac
   101821 ttatttaaaa agcgttgata tcccactcta tctaaatcag aaatcaatac cgctggcata
   101881 tatttcttta tgttcattaa cgtatcttcg tattttctat aagtgatagg agatactgtt
   101941 ccttctttat aaagttgcat ccaatcctca aagtaatctg ataataatag atttttgcga
   102001 tcccctttaa ggcctttgtt caagttatat tctaattcgt tggcggcatc ttttgcttct
   102061 ccttttgtct taaatcctga ttttcttatc ttcgtgtatt ttccgtcatc ttttttgtat
   102121 gaaatttcgt attgccaact atttccacgt ttgactaatc ttgccataat tgaatcagct
   102181 ctctttctct gatacaatag gaactataaa gaagcccatt gtttgggttt tattattttt
   102241 tgcacatcct ttttgcttgc cggcgcaagg atgtgctatt tgtcgtttta atggtataat
   102301 tggatgtgta ttagatcatc caatcagagg tgtttattat gattaaagtg atttttaaaa
   102361 gaataactga acttccagag tttagattat ttgttgatta ttggtatgtc tggtttttta
   102421 ttgttgcatt gctgataggt ctagatgtca tattagaaag aaaacttaag taagagttga
   102481 gttaataaat tgaattccta aatgagatat tttgtagccc atattttgtt tgccgacatc
   102541 acctaatact atggagctaa tcgttttttc taacagacca aaatcagata atctatttaa
   102601 gctaccatct attagctctt gtgaatagcc agacatgctg atatttccag tgaaattttc
   102661 gtttccacga ctcattaagc atgctaacaa ttgaatctca atcgatgtta attgattcag
   102721 tacaagaaga taatactcct cgttatccca tgaagcgttg ttaattcgaa gtaaacaatt
   102781 cttataaaga tttttataca tatctatttt attttgtgcc tttgattttt ctacctcgtc
   102841 atttatccct tcgaatattc caatgagttg gtccttgttc tctaagttga aaacatcttc
   102901 tggaatttga tcgcgaatgt tttctaatga ttcgtttaaa tttttgtaga atttctcgat
   102961 gcgtttgaaa cgcttctcgt ttttagaccc aaaataaagt gactgcacaa cgccaccaat
   103021 ataaggaatt gctccaatac cgccttcaac tattaattca agatattctt ttttggttag
   103081 tttttcattt ctattatcca tagttacctc ctattttgtc atttccttta caagcattgt
   103141 gactcctgat ggcactcttt tgtataggtt ttattattgt gcacaatcta actcttagtg
   103201 gggcgtggtt catttatttt ttacttactt tactattaat catatcccat tgagcatagg
   103261 atttttctat gttcgatcct cttgaactaa tttctcctgt aaaagttact ttatcgccct
   103321 ttgtaaaggt atcagcattg tttaagtcct tcgcaaaaat tacatatgaa actttaggtg
   103381 ttgcagaaat atcattccaa cttttatcat cgtactcttt gtctgctata atggctattc
   103441 tagtagtcat tgattcgata acagttcctt caaaagtata tgttttacca ctaatatatt
   103501 tagtaaaagc tgtattttgc tcagtagctg gagtaataga ataataacta tctaagaaag
   103561 caccccagtc atgattattt gtctctaaaa attcttcaat ttgagttagg cttttattgc
   103621 tgctgttaga tgtagtagtt tgagtatcag atgatgagct ttcctgtttg ttattgtcat
   103681 ttgcctcgtt tgatgaggaa agcgagaaac ctacggtagt aaaaataatc ccgataataa
   103741 ggagaatcat aggtgttttc ttatttgttt ttttgataaa tcttacaatt aacatgatta
   103801 aacctataat aaataaaaca ataccagtaa aaccaaatat accgtccatt aatagatcct
   103861 ctttctcttt tgatattgaa tattataaac caagcaattc ttttttctta aaatcgaatt
   103921 cttcttgatt aataatccct tcatctaaca actctttata ctttttaatt tcatctgcac
   103981 ttgaaatttg tgtttctttg gaattattat ctaatataga ttgttgcttc tctatatatt
   104041 tttttaaatt ttccatttca ttccaatatt tttttgtaaa cataacggtg ttttcatctt
   104101 tcgtggccgc taatatacct ccgcgacttt cgtttccgcc tagcaaggta aattgtatat
   104161 aaccattact catacctggt ttttttaatt gtacggctga tatattttta aaggggatag
   104221 ttttttctcc tttaatacct tgattaacta aatttataaa tccttttcta ataattgaaa
   104281 tagaatcatc agttacttta atcaaggttt ttcctggaga ttttattaaa atctcttttt
   104341 ccattttttc ttcctcattt ctgtgatatg attttttttg tagaagctca tatatgagga
   104401 aagagcccgt gttgcagcac gggctttttt aatgttttgc tgaaatcggt ttttttaata
   104461 attcttgatg tattcataca tattgccttg cgtaagaata ttttttctta aaatagcatt
   104521 ggcagacagc ataacaagtg tatctgagct tattctgaat agaataatac tccataaatt
   104581 tttcgagatt aaactgagat tcatcagtca gttcgttctc aatatagata tttaataaaa
   104641 ttagaatagc tattttatct gcttctgttt caaattttga gtgaaaagtt gtagaagtat
   104701 cgtacaaaac tgaaaattca aaatgagagg cgctgaaatg tgcaagttca tgagataaat
   104761 gaaaggcttc tgcagtttca ccgtatagat tttcattcaa aaaaatgatt ctgggttttg
   104821 gatagtagaa accaggctct ttcatctcca tataaactac tttcaaattg tattcgctca
   104881 acatttcttt caatttcaaa tacatacaaa ccatcactcc aactattcat tttcctctaa
   104941 agctttagca attgcaatcg ctttacgcat tgtctcctta gatatttctt ttccatcaaa
   105001 agaaaaaaca gtatcgtctt ctgataaatc cacatgttta ggggtttctc tttcttctct
   105061 acctagaagg tagtctacag agacatcgaa atagtcagca atccgactta attcatctga
   105121 attaggagta ttatttttcc actttgctag ataaccgtta gaatatccaa aattaatttc
   105181 taactgccgt atagatatct ttttcctctt cgccaagtct tttattattt cataggtatt
   105241 catcgataaa acaacctttc taaatgttta caaaaaaagt ttagaaaaat acgcagaaat
   105301 aatttgacta attctgagta atgagctata ctatatcttg taaacagatt taacaactaa
   105361 aaagacaaca aaaaacacta ttgatatgta aatgcagacc gccaagaaag ctttaaaatc
   105421 aatgttttta tgtcttattt aattatgctc ttagtataga gtattactca gatactgtca
   105481 attgaattta gaaaaaagtt gttaaatttg tttacgaata taaaagaaag gaatgagaaa
   105541 tatgaacaca ccgcaaattt ttaatttcga acaaaacgaa gttcggacaa ttttagtaaa
   105601 tgatgaacca tattttgtag gcaaagacgt tgcgagtgtt ttgggttatt caaacactaa
   105661 agatgctttg tcgcggcatg tagatttgga agataagatg gggtcgcgaa tcacgacctc
   105721 aggtcaatca agagagatga caatcatcaa cgaatctggt ttgtacagtt taatcttaaa
   105781 atcaaaactt ccctctgcca aaaaatttaa acgttgggta acaagtgaag tgttgccagc
   105841 aattagaaaa catggaggtt atctaactcc agaaaaagta gaagaagctt tgcttaatcc
   105901 agatacaatc attcaattag caactcaact aaaagaagaa agaactggaa gattaatcgc
   105961 agaacaaaag attgcagagt acgaacctaa aatctcctat ttagatagca tattatcttc
   106021 tacagattca gtaacaatta gtcagattgc agcagattat gggatgtctc cacaacagat
   106081 gaataaatta cttcataaac taggtattca gaaaaaagtc ggtaaccaat ggttattgtg
   106141 caaaaaacac atgaaccaag gatacacaaa atctcataca actgagatcc cgaaagccga
   106201 tggtggcact aaaattgtaa tgaataccaa atggacacag aaagggcgtc tatttatcta
   106261 cgaattacta aaaaaagaag gatattaccc tcaaatggat ttagaggaaa ttggttagaa
   106321 aggagtttta gtatgactga cattgcagaa atcactcaac gagatagaga aaaaatcaaa
   106381 gaatatgtcg aaagttcaaa gttcttaact tacaccatgc ttgctgaaag atttggaatt
   106441 agcaaaagct acttatcttt aattttaaac ggtaaaaaga cttctgcaga agcaaacaga
   106501 attatagatt cgattatcac tatgtacgaa ttgtaaaggg aggaacagct aatgcaatat
   106561 ctagaagcaa aaatcccaat tccagaaggc tatgtaatta tctcccaagt ggattatgag
   106621 gagttaaaaa aagctgatga tactggtaga tggatgacgt tgccagaagt gctagaacgg
   106681 attaacagaa aatatgattg gtttacttct agagttttaa agaacccaag atatagaaat
   106741 attatcgata tcgagaaaaa caaaaatgga tttgtctatt atccagttga aggaagagac
   106801 acatatctat ttttaagaag taaaacactt gaatttttag aaacaaattt ttcggaaatc
   106861 ttaaggaggc aagcggatgg gaaaatttaa tagagcgcta gtgttcagcg caccgctaat
   106921 catctacgct ttaggacttt ggggaagcag
//
LOCUS       DANKEF010000002       101956 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000002 DANKEF010000000
VERSION     DANKEF010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 101956)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 101956)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..101956
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.002"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(553..2166)
                     /locus_tag="PWZ48_000113"
     CDS             complement(553..2166)
                     /locus_tag="PWZ48_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2259809.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2307..3215)
                     /locus_tag="PWZ48_000114"
     CDS             complement(2307..3215)
                     /locus_tag="PWZ48_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2259810.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3398..4522
                     /locus_tag="PWZ48_000115"
     CDS             3398..4522
                     /locus_tag="PWZ48_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2259811.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4749..5666
                     /locus_tag="PWZ48_000116"
     CDS             4749..5666
                     /locus_tag="PWZ48_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2259812.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5659..6492
                     /locus_tag="PWZ48_000117"
     CDS             5659..6492
                     /locus_tag="PWZ48_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2259813.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6575..7693
                     /locus_tag="PWZ48_000118"
     CDS             6575..7693
                     /locus_tag="PWZ48_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2259814.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7687..9585
                     /locus_tag="PWZ48_000119"
     CDS             7687..9585
                     /locus_tag="PWZ48_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HDL2259815.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9579..10802
                     /locus_tag="PWZ48_000120"
     CDS             9579..10802
                     /locus_tag="PWZ48_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2259816.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10888..12822)
                     /locus_tag="PWZ48_000121"
     CDS             complement(10888..12822)
                     /locus_tag="PWZ48_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2259817.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13048..13704
                     /locus_tag="PWZ48_000122"
     CDS             13048..13704
                     /locus_tag="PWZ48_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2259818.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13778..14131)
                     /locus_tag="PWZ48_000123"
     CDS             complement(13778..14131)
                     /locus_tag="PWZ48_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2259819.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14323..15252
                     /locus_tag="PWZ48_000124"
     CDS             14323..15252
                     /locus_tag="PWZ48_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDL2259820.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15263..16099
                     /locus_tag="PWZ48_000125"
     CDS             15263..16099
                     /locus_tag="PWZ48_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HDL2259821.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16347..16721
                     /locus_tag="PWZ48_000126"
     CDS             16347..16721
                     /locus_tag="PWZ48_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2259822.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYGVSIKS"
     gene            16779..17905
                     /locus_tag="PWZ48_000127"
     CDS             join(16779..17007,17007..17905)
                     /locus_tag="PWZ48_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2259823.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAGSHILSENLNSLVQFIEKWCKDYTVS
                     LLCRLLEIPRSVYYFYKNKPLTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKE
                     GISVSLKHVQKLMKQLNLRSIVVKKYRPQRSNKPIISKENLLNQDFSTETICEKWAAD
                     ITYIPTKKNGWCYLSSIMDLHTKKIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMIL
                     HTDLGSQYTAREVEQWLKTNKIRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDF
                     EEANRALFSYIEGFYNRNRIHSSIHYLTPQEFEELAKEKMA"
     gene            17986..18375
                     /locus_tag="PWZ48_000128"
     CDS             17986..18375
                     /locus_tag="PWZ48_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family transcriptional regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HDL2259824.1"
                     /translation="MKEKENYPVQHATKKRWTTVRYGNSDHYIHYDEILYLMSMPGYK
                     NKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENITSISRVNEIVTFAN
                     GEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            18551..19294
                     /locus_tag="PWZ48_000129"
     CDS             18551..19294
                     /locus_tag="PWZ48_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2259825.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            19287..20867
                     /locus_tag="PWZ48_000130"
     CDS             19287..20867
                     /locus_tag="PWZ48_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HDL2259826.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(21118..21597)
                     /locus_tag="PWZ48_000131"
     CDS             complement(21118..21597)
                     /locus_tag="PWZ48_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2259827.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(21613..22365)
                     /locus_tag="PWZ48_000132"
     CDS             complement(21613..22365)
                     /locus_tag="PWZ48_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HDL2259828.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            22482..22552
                     /locus_tag="PWZ48_000133"
     tRNA            22482..22552
                     /locus_tag="PWZ48_000133"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:22514..22516,aa:Gly,seq:ccc)
     gene            22913..23638
                     /locus_tag="PWZ48_000134"
     CDS             22913..23638
                     /locus_tag="PWZ48_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2259829.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            23893..24159
                     /locus_tag="PWZ48_000135"
     CDS             23893..24159
                     /locus_tag="PWZ48_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HDL2259830.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            24156..24587
                     /gene="ruvX"
                     /locus_tag="PWZ48_000136"
     CDS             24156..24587
                     /gene="ruvX"
                     /locus_tag="PWZ48_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDL2259831.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            24612..24917
                     /locus_tag="PWZ48_000137"
     CDS             24612..24917
                     /locus_tag="PWZ48_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HDL2259832.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(24998..26143)
                     /locus_tag="PWZ48_000138"
     CDS             complement(24998..26143)
                     /locus_tag="PWZ48_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2259833.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(26204..26851)
                     /locus_tag="PWZ48_000139"
     CDS             complement(26204..26851)
                     /locus_tag="PWZ48_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2259834.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            27045..27338
                     /locus_tag="PWZ48_000140"
     CDS             27045..27338
                     /locus_tag="PWZ48_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259835.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            27382..27693
                     /locus_tag="PWZ48_000141"
     CDS             27382..27693
                     /locus_tag="PWZ48_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259836.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            27690..28314
                     /locus_tag="PWZ48_000142"
                     /pseudo
     CDS             27690..28314
                     /locus_tag="PWZ48_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            28455..28613
                     /locus_tag="PWZ48_000143"
     CDS             28455..28613
                     /locus_tag="PWZ48_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259837.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            28614..28976
                     /locus_tag="PWZ48_000144"
     CDS             28614..28976
                     /locus_tag="PWZ48_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259838.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            29013..29861
                     /locus_tag="PWZ48_000145"
     CDS             29013..29861
                     /locus_tag="PWZ48_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HDL2259839.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            29851..31326
                     /locus_tag="PWZ48_000146"
     CDS             29851..31326
                     /locus_tag="PWZ48_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HDL2259840.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            31592..31999
                     /locus_tag="PWZ48_000147"
     CDS             31592..31999
                     /locus_tag="PWZ48_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HDL2259841.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            32002..32217
                     /locus_tag="PWZ48_000148"
     CDS             32002..32217
                     /locus_tag="PWZ48_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259842.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            32221..32601
                     /locus_tag="PWZ48_000149"
     CDS             32221..32601
                     /locus_tag="PWZ48_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2259843.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            32735..32890
                     /locus_tag="PWZ48_000150"
     CDS             32735..32890
                     /locus_tag="PWZ48_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HDL2259844.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            32959..33432
                     /locus_tag="PWZ48_000151"
     CDS             32959..33432
                     /locus_tag="PWZ48_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HDL2259845.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            33429..35123
                     /locus_tag="PWZ48_000152"
     CDS             33429..35123
                     /locus_tag="PWZ48_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2259846.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            35089..35274
                     /locus_tag="PWZ48_000153"
     CDS             35089..35274
                     /locus_tag="PWZ48_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259847.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            35278..36453
                     /locus_tag="PWZ48_000154"
     CDS             35278..36453
                     /locus_tag="PWZ48_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2259848.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            36446..37969
                     /locus_tag="PWZ48_000155"
     CDS             36446..37969
                     /locus_tag="PWZ48_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2259849.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            38025..38309
                     /locus_tag="PWZ48_000156"
     CDS             38025..38309
                     /locus_tag="PWZ48_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HDL2259850.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            38296..38631
                     /locus_tag="PWZ48_000157"
     CDS             38296..38631
                     /locus_tag="PWZ48_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2259851.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            38926..39552
                     /locus_tag="PWZ48_000158"
     CDS             38926..39552
                     /locus_tag="PWZ48_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259852.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            40294..41130
                     /locus_tag="PWZ48_000159"
     CDS             40294..41130
                     /locus_tag="PWZ48_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDL2259853.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            41194..41625
                     /locus_tag="PWZ48_000160"
     CDS             41194..41625
                     /locus_tag="PWZ48_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2259854.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(41687..43009)
                     /locus_tag="PWZ48_000161"
     CDS             complement(41687..43009)
                     /locus_tag="PWZ48_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HDL2259855.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(43163..44803)
                     /locus_tag="PWZ48_000162"
     CDS             complement(43163..44803)
                     /locus_tag="PWZ48_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HDL2259856.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            45136..45606
                     /locus_tag="PWZ48_000163"
     CDS             45136..45606
                     /locus_tag="PWZ48_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HDL2259857.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            45828..46931
                     /locus_tag="PWZ48_000164"
     CDS             45828..46931
                     /locus_tag="PWZ48_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2259858.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            47215..47895
                     /locus_tag="PWZ48_000165"
     CDS             47215..47895
                     /locus_tag="PWZ48_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2259859.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            47985..49019
                     /locus_tag="PWZ48_000166"
     CDS             47985..49019
                     /locus_tag="PWZ48_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2259860.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            49033..49368
                     /locus_tag="PWZ48_000167"
     CDS             49033..49368
                     /locus_tag="PWZ48_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259861.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            49352..51832
                     /locus_tag="PWZ48_000168"
     CDS             49352..51832
                     /locus_tag="PWZ48_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259862.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            51888..53447
                     /locus_tag="PWZ48_000169"
     CDS             51888..53447
                     /locus_tag="PWZ48_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2259863.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            53444..54808
                     /locus_tag="PWZ48_000170"
     CDS             53444..54808
                     /locus_tag="PWZ48_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2259864.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            54978..56048
                     /locus_tag="PWZ48_000171"
     CDS             54978..56048
                     /locus_tag="PWZ48_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2259865.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            56063..56731
                     /locus_tag="PWZ48_000172"
     CDS             56063..56731
                     /locus_tag="PWZ48_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2259866.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            56751..57329
                     /locus_tag="PWZ48_000173"
     CDS             56751..57329
                     /locus_tag="PWZ48_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2259867.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            57730..58641
                     /locus_tag="PWZ48_000174"
     CDS             57730..58641
                     /locus_tag="PWZ48_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2259868.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(58729..59646)
                     /locus_tag="PWZ48_000175"
     CDS             complement(58729..59646)
                     /locus_tag="PWZ48_000175"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HDL2259869.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            59848..60285
                     /gene="zapA"
                     /locus_tag="PWZ48_000176"
     CDS             59848..60285
                     /gene="zapA"
                     /locus_tag="PWZ48_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDL2259870.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            60344..60892
                     /locus_tag="PWZ48_000177"
     CDS             60344..60892
                     /locus_tag="PWZ48_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HDL2259871.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            61007..63367
                     /locus_tag="PWZ48_000178"
     CDS             61007..63367
                     /locus_tag="PWZ48_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HDL2259872.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            63454..63768
                     /gene="trxA"
                     /locus_tag="PWZ48_000179"
     CDS             63454..63768
                     /gene="trxA"
                     /locus_tag="PWZ48_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HDL2259873.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            63950..65728
                     /gene="uvrC"
                     /locus_tag="PWZ48_000180"
     CDS             63950..65728
                     /gene="uvrC"
                     /locus_tag="PWZ48_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDL2259874.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            65873..66772
                     /locus_tag="PWZ48_000181"
     CDS             65873..66772
                     /locus_tag="PWZ48_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2259875.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            66765..68027
                     /locus_tag="PWZ48_000182"
     CDS             66765..68027
                     /locus_tag="PWZ48_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2259876.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            68188..70086
                     /locus_tag="PWZ48_000183"
     CDS             68188..70086
                     /locus_tag="PWZ48_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HDL2259877.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRISSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(70416..71135)
                     /locus_tag="PWZ48_000184"
     CDS             complement(70416..71135)
                     /locus_tag="PWZ48_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HDL2259878.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            71472..71804
                     /locus_tag="PWZ48_000185"
     CDS             71472..71804
                     /locus_tag="PWZ48_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259879.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            71791..73794
                     /locus_tag="PWZ48_000186"
     CDS             71791..73794
                     /locus_tag="PWZ48_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HDL2259880.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            73813..74283
                     /locus_tag="PWZ48_000187"
     CDS             73813..74283
                     /locus_tag="PWZ48_000187"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HDL2259881.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            74304..74888
                     /locus_tag="PWZ48_000188"
     CDS             74304..74888
                     /locus_tag="PWZ48_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HDL2259882.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            74903..75889
                     /locus_tag="PWZ48_000189"
     CDS             74903..75889
                     /locus_tag="PWZ48_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HDL2259883.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            75879..76190
                     /locus_tag="PWZ48_000190"
     CDS             75879..76190
                     /locus_tag="PWZ48_000190"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HDL2259884.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            76212..77993
                     /locus_tag="PWZ48_000191"
     CDS             76212..77993
                     /locus_tag="PWZ48_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HDL2259885.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            77986..79362
                     /locus_tag="PWZ48_000192"
     CDS             77986..79362
                     /locus_tag="PWZ48_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HDL2259886.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            79369..80004
                     /locus_tag="PWZ48_000193"
     CDS             79369..80004
                     /locus_tag="PWZ48_000193"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HDL2259887.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            80034..81389
                     /locus_tag="PWZ48_000194"
     CDS             80034..81389
                     /locus_tag="PWZ48_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HDL2259888.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(81581..82975)
                     /locus_tag="PWZ48_000195"
     CDS             complement(81581..82975)
                     /locus_tag="PWZ48_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2259889.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            83067..84038
                     /locus_tag="PWZ48_000196"
     CDS             83067..84038
                     /locus_tag="PWZ48_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HDL2259890.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            84035..84817
                     /locus_tag="PWZ48_000197"
     CDS             84035..84817
                     /locus_tag="PWZ48_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HDL2259891.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(84986..85357)
                     /locus_tag="PWZ48_000198"
     CDS             complement(84986..85357)
                     /locus_tag="PWZ48_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2259892.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            85491..85934
                     /locus_tag="PWZ48_000199"
     CDS             85491..85934
                     /locus_tag="PWZ48_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDL2259893.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(85977..86786)
                     /gene="yidA"
                     /locus_tag="PWZ48_000200"
     CDS             complement(85977..86786)
                     /gene="yidA"
                     /locus_tag="PWZ48_000200"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDL2259894.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(86805..88172)
                     /locus_tag="PWZ48_000201"
     CDS             complement(86805..88172)
                     /locus_tag="PWZ48_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2259895.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(88156..88986)
                     /locus_tag="PWZ48_000202"
     CDS             complement(88156..88986)
                     /locus_tag="PWZ48_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HDL2259896.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            89149..89586
                     /locus_tag="PWZ48_000203"
     CDS             89149..89586
                     /locus_tag="PWZ48_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HDL2259897.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            89662..90282
                     /locus_tag="PWZ48_000204"
     CDS             89662..90282
                     /locus_tag="PWZ48_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HDL2259898.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            90471..90977
                     /locus_tag="PWZ48_000205"
     CDS             90471..90977
                     /locus_tag="PWZ48_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HDL2259899.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            90994..91893
                     /locus_tag="PWZ48_000206"
     CDS             90994..91893
                     /locus_tag="PWZ48_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HDL2259900.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            91980..92864
                     /locus_tag="PWZ48_000207"
     CDS             91980..92864
                     /locus_tag="PWZ48_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2259901.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(93056..93127)
                     /locus_tag="PWZ48_000208"
     tRNA            complement(93056..93127)
                     /locus_tag="PWZ48_000208"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(93092..93094),aa:Arg,seq:ccg)
     gene            93187..94521
                     /gene="rpoN"
                     /locus_tag="PWZ48_000209"
     CDS             93187..94521
                     /gene="rpoN"
                     /locus_tag="PWZ48_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDL2259902.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            94670..95761
                     /locus_tag="PWZ48_000210"
     CDS             94670..95761
                     /locus_tag="PWZ48_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HDL2259903.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            95802..96803
                     /gene="gap"
                     /locus_tag="PWZ48_000211"
     CDS             95802..96803
                     /gene="gap"
                     /locus_tag="PWZ48_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2259904.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            96913..98103
                     /locus_tag="PWZ48_000212"
     CDS             96913..98103
                     /locus_tag="PWZ48_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDL2259905.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            98207..98962
                     /locus_tag="PWZ48_000213"
     CDS             98207..98962
                     /locus_tag="PWZ48_000213"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDL2259906.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            99078..100376
                     /gene="eno"
                     /locus_tag="PWZ48_000214"
     CDS             99078..100376
                     /gene="eno"
                     /locus_tag="PWZ48_000214"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDL2259907.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            100775..101914
                     /locus_tag="PWZ48_000215"
     CDS             100775..101914
                     /locus_tag="PWZ48_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HDL2259908.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        33885 a        17402 c        21000 g        29669 t
ORIGIN      
        1 gttaaataaa taggaacaaa aaaattaaat tatttaatac acacattagg gatcattcat
       61 tgaaggatta gaagacaacg aggatggcga aaaatatgat tacaatagaa aagtcgttct
      121 aaaccaatca gttcctagta tcacacaacg caagccagtc gatttggtga gattagagga
      181 gtgaaagcaa actttattat ataagaaaaa atattcgaaa gggatgctag tttaggtact
      241 agtgctgata gattaatatg atgcctcgta atattaggcg tagttactct agagacatca
      301 ctatggacta tgatttgtct cagagtcaca acatgttttt ttatgaaaag aaacagctaa
      361 aataattaaa ttgcgcaatg tttaaacttt cttaaactat aacctaataa atgtgaagaa
      421 ataatgcaca atttcttcac atttatcttc tattctatat acataccaac caacaaccct
      481 atatactttt tcatttttac ttgaggagta aaaataactc ctagaccgcg gctacccacc
      541 gcggtcgttt ttttattctt caatcaaaat cgctttgata cccaaacctg ccaagattcg
      601 ggcagaagta taagtattca atcctcttct gaaagtaatg taaacttttt tatcttttgg
      661 gatttcattg attcgatctc gtaactcatt cattggaata ttttttgttg ccgagatact
      721 gccagaaggt gctttattag gttcacgtat atctaaaaag aacgcttctt tcaaatcgtt
      781 ttcgggtata tcacttgctt tgatcgtttc tacgatattc gtcatttgat tgattgcgac
      841 ataaccagct atattcaacg ggtcacgggt agtagaatac ggtggagagt aaggcaactc
      901 caaatcaggc aaatcaaaga ctgtcaagtt tccggtgatt gctacagaaa gttctcccat
      961 ccgtttatcg acaccttttt ctccaaatgc ttgtccacct aaaatacgac cgctatctgc
     1021 atcaaaaatc agctttagat ttaatcttgt agcttctgga tagaaatagg catggtcaaa
     1081 gggagtgata aaaacagttt tataatttgt tatgcctgat gcttttaatt catgttctgt
     1141 cattcccgca taacttgctg tatgatcaaa gatttttgcg acacctgcac caatatatcc
     1201 gcgatagcgc ataggcgtac catttaaaat atctgccagc atatgtcctt gacgattggc
     1261 cgcactggaa agcatacttt ggatcggctg accagtcact acactagtcg tttcgataat
     1321 atcgccaatc gcatagatgt ccggcagatt ggtttgtaac tggtcatcaa caatgatctg
     1381 acctgtatct gataattgta tgccggttgc tttcaccact tcattcttcg gagaaacacc
     1441 aacagcaaaa attagcatat cagcagaaag gacggagcca tctgataata ctatttctcg
     1501 acctttatca cggatctcag taactcttgt ttctaaatga acggctaatc cttctttcag
     1561 caatttgtca taaactaaat tagcaatttc tggatcataa gggaaagcga cttgtggcaa
     1621 ttgatcgatt aaagtcacgt ctaagccgcg atgcttgaaa ttctcagcta attccagacc
     1681 cataacacct gcaccaagaa tgacgacatg ttgtggattt ttttcttcaa ggaacgattt
     1741 gattcgatcc gcatccgtca cactacggag gacaaagccg ttttctgctt caaaaactcc
     1801 gggaatatcc ggataatctg gtctagcacc agaagagatg atcaacttat cgtaatctgt
     1861 ttttatttct tcatttgtgg ataggtcttt aactgttaat cgcttagtag aaggatcgat
     1921 tgctgttact tcgtgtttag taaagacatc gatgttgttt tttgttttca atatttctgg
     1981 tgtacgttcg atcagcgagt cacggtcagt gatcacacct cctaaataat aaggcaaagc
     2041 acaacttgca taagaaatat tttctccgcg ttcatagata atgatttcgt gcgcttcatt
     2101 caatcgacgg aaacgagtgg caaatgaagg accgccagct acaccgccaa tgacaacaac
     2161 tttcataaaa acatctcctt ttctacgttg atcgtactat gttatggtaa aaaacagcaa
     2221 ataattgtgc cggaaaatta ttgtttattt tgaatagaca gaaaatatag agaaataatt
     2281 tgtggataaa acctaaaaat ttttttctat tttaaggata gataatactt tgaaggggat
     2341 aaccctgtat attttttaaa agctgaagaa aaatgatgga tcgaatgaaa tcctaatata
     2401 tctgcaattt ctgtataggt taattcattt tctagcacga gacgttttgc tttttccatt
     2461 tttagttctt taaaatagct aataacactt tttccattca cttttctaaa atgttttttt
     2521 aagctggctg gactgataaa aaaaatttgg gctaattgtt caagcaaaaa attttctgaa
     2581 agatgttctt ctaaatatcg tatgattgga tcaactaatt catctgaata aagaatatgc
     2641 ttttttttag gaatgacttt ctcatttgaa ggtattgtca ataaagcaag ttcctgttga
     2701 taatgccttg gtaaggaaga caattgaatt ttttgcagac aataagtaac taataaggaa
     2761 agcaaatgaa tgctcatctg ttggcatagt atgaaaaaag aattgatttc ctgatcatag
     2821 gaaatagctg gcttactatg ttttagataa tcaattttga actgttcatt tttcagaaaa
     2881 gtaatgatag atgttatttc ttgatctata gaaaaagatt gtcccgtaat atgtgctaat
     2941 aattgctcat caaaataaaa ctggataatg ataaaagttt gtgtttcctt ttgagtagaa
     3001 aagatttgga cttcttgctg tttatcaatg aataaaaggg aaggattatc tagttctata
     3061 gatttatgtg caaattcagc atataaatta gcgctttcat ttattagtag tcgaaaatag
     3121 ggagcagcag ataaatgaag atgggtatct atggaatagg ttagtgtttt tttgacaaga
     3181 ttggaggatt ggttgtttgt taacaaaaga accactttcc tttctctttt tatataaaaa
     3241 gctttctatt tataattaat gactattata acaaaaatga aaagccaaaa cgacaagtaa
     3301 aatatttttt tatataaata tttgtgttac taattctgtt aatatacata agtgaaagcg
     3361 ttttatataa gaacttgaaa agaaagaagg gatagacatg aaaaaaagat acaaattggt
     3421 gatagctttg ctaaacatta ccctattatt cagcttaagt gcatgtggaa aaactaacga
     3481 agaagtaaca gcaaacatcg cagatccaat gataaaaaaa gccactacat tagaagtata
     3541 tgtcgcaaac ggcaaactga caaactatgt tgaaaatgct gcaaaaatct ataaccgcaa
     3601 aactggagca aatattgact taaaaattgt gaatgtagct tcgggaacag caacagtaca
     3661 aatgatcacc ccaaaattgg taacacacga agatatgccg gatattatct ccatggcaga
     3721 cacttcagca gcaggtgtgc tggaaaaatt tcaagatgct ttttatagtg ctgatgaata
     3781 tggattttat gacaaatatg gcgatcagtt ttatgatcaa aaattgaata ttttgcgtca
     3841 acaaacgagt aatgaaacga ttattccttg ggctagtgat tttaccccag cactttgtta
     3901 ttatcaacca aaaaaatttg ctgaaatagg tgtagatttt gaagaaatca gttcttggga
     3961 agaatatatc caggtggcaa aagcattaaa agaaaaaact ggtagttttg gaattgcctt
     4021 gccagaatct ggggaccaag aattgtttat caacatgcta gcccagcaga atcaatcttt
     4081 gcttgatgaa gaaggaaata tccgtctgaa tactaaagaa gcgaaacatg ctgcaaagtt
     4141 aatcaaagaa atgatcgact cagatattgt acatttctac ggtgcacaag atgctgagaa
     4201 agcatttcaa gaatctgcaa tgtttgttgc tggcggctgg tatgctacga atatgtcttt
     4261 gaattttcct gatgcatctg acaaatggcg catggctcca ctgattcctt tttcaaaaga
     4321 aaatccagga aaaggtgcag tatcaggagg atcaagtttt tatgtcccta aagatgcaaa
     4381 aaatccacaa gtggcacaac aatttcttac atttatgctg acagatgaag aatgcttagc
     4441 caacgcactg gagctgggcg tggctacatc taactcattg gcctatcaaa cagaagcagc
     4501 tgagaaaaag tttgactact aaggaggaca aaaatactat gaactattga ataaaatcaa
     4561 tgaaaataca tcaaatactg ttttaccagc cacttattca gatgctacgt cttatttgac
     4621 taccgctgca tataactatt ggcaaacagg tgattttgaa aattcatatc taaaagaagc
     4681 agaaaatttt gctcataagt acggcgtaaa attaactggt gattaaaaaa tattaggagg
     4741 acttaacgat gatgcaatct tcgttaccaa gcaagacaat aaagaacagc aagaaaaaag
     4801 caacagatac gtctcataaa aaaagtgctt atggattggt cacacctttt gttattttct
     4861 ttattgtgac gattttgatt cccatcatta tctcgtttgc ttttagtttt atgaaaattg
     4921 gctactcatt tgaatttgtt ggatttgaca attatctagc tatcttccga gatccattgt
     4981 ttattaaatc ttacacgaat gttttgatta tcatggcagg atcaattcca ataacgatgc
     5041 tattagctat ttactttgct gttttattga attcagcaca gttaaaagga aaaggatttt
     5101 ttagaactgt ctattatatt ccaaccatta catcaacggt ggctgtagct tccgtattca
     5161 tgactttttt caatccgact gggttattca atggattttt gaatgcgcta ggaattaacg
     5221 gtgtacagtg gttaacaaat cctttttgga tacgaatatc aatgatcatc acgatgattt
     5281 ggcttagtgt aggatataac actattttat ttttagctgg tcttcaaggg atttctaaag
     5341 agatatacga aagtgctgaa atagatggag catcgaaatt tcggcaattc tttttattaa
     5401 cgatccccat gctaaagcca atcattttaa tggccacagt tttagccact atcaatggct
     5461 tgggtaattt caatatccca aatatattct ttgggacttc caatggacca gaaaattcag
     5521 ctttggtcgt aggagtcaat ttatataaaa caagttttga aatggttgat tttggaaaag
     5581 cctctgctat tgcctggacg atggtgtttg tatctgttat attatcggct attcaattta
     5641 aattcggagg tgataaaaat gactaaacaa aagatacaaa gcagaatttt tacgtacgtc
     5701 attattgtgt ttggtgcctt aatatttttg atgccatatt tatatatgat tgtagcttca
     5761 acgcaaagta atcaagaaat tttaagtggc ggaatcaatt tcaagtttgg ggatatgcta
     5821 ttaaaaaact ggcaagccat catggcgaga tataattatc ccttgattat ttggaattcg
     5881 attgttattg ctgtaatcag tacagtttta gctacagcca gttctacaat ggcgggttat
     5941 gcattagcaa agtatcgttt tatggggaat aaactgttgt tcaatacagt tatgctatct
     6001 aggatggtgc ctttttttac cacattgatt cctttatttt acattatgag tcgtttagga
     6061 ttatcgaata cttatgcagg tgtgatcatc ccttcgattg ccagtacaac ttcagtgttt
     6121 atgatgcgac aatatagcag tcaatttcct acagcactga tggaggctgc cagaatcgat
     6181 ggtgccaatg agtgggtgat ttttacgaaa atcgctgtac cgattttatc acctacgatt
     6241 attactagtg gtttacttat ctttatggga agttggaatc aatatctatt tccattagtc
     6301 atgctttcag ataaagataa atatacggtt cccttaatta tcaaaaatat gacggtttct
     6361 agtacaggtg agccaattaa ttatggcgca attatgctaa ttttagcaac ttcagtgatc
     6421 ccaatggtct tgatttatgt gtgggcgcag tctaaattta aggagaatga cattggagct
     6481 gcaaataagt aaaaaaaata acgaatgtta accatctagt tcgttctagg agcaactcca
     6541 cattcataaa aattaaaaga atggcgggat atccatgaat gaacagacga aggaatggtc
     6601 aagaaaaatt ttgaatgtag cttctgaaaa gatagatgcg gaattagaca gaatggggaa
     6661 taaaattcca tatatttctt acaacggaaa atatacagac gtaataaagg acagaggaat
     6721 cgactggtgg accaatggtt tttggagtgg tattttatgg cagctctttc acaagacaaa
     6781 acgagaaaag ttcaaagagg cagcaataca acaagaacaa ttattagaat ctgccctgta
     6841 ccatttttca gacgttcatc atgatgtagg ttttttattc ttaccatcgg ctattgcaca
     6901 ttatcaacag tctcaaaaca aacatagtcg acaactaggg ttacatgcag ctacggtact
     6961 tgctggacgc tataatattt cagggaacta tttagtagca tggaatgacc ataaacctgg
     7021 ttggatgatt attgatagta tgatgaattt accattactg tattgggctt cgagggaaac
     7081 tggtgatccg aggttctcta aaattgctcg gcagcatgca gatacaacag ctaagtatct
     7141 agtgaggaaa gatggatcaa ccgggcatat cgcagaattt gatgcagaat cagggaaata
     7201 tattcggtta cttggcgggc aaggatacaa tgagaattct tgttggagca gaggaaatgc
     7261 atgggcttta tatggattcg cgttaagtta tcgttacaca aaaaatccgt actatctagc
     7321 tatagcagaa aagatagcag taaattttat tcagaaaatc gaaaaaactg gttatattcc
     7381 tctcattgat tttgctgtaa aagattcgaa tattgatacc agtgctggag tatgcgctgc
     7441 atgcggtctt ctggaattag caggatatgt ttcctctgta cgatcaaaaa tgtatcatga
     7501 agctgctatc aatatactcc aaaagacaga aaagcactat gcaaattgga atatggatga
     7561 agatgggatc attggaggcg gagcagaagc ataccataga gaactgactt ataatgtgcc
     7621 actgatctat tctgattatt tttttattga aggcttactt cgattagaac agtcggaatt
     7681 agctatttgg tagtaaaaag aggagaaaat acaatgaaac atccagtacc actattaagg
     7741 accagacagg attactttga cttatttaaa aagataaatc aaccactcca gccgttttat
     7801 cgagcaagtg gcgcacaaat tgattatata catcaagggg taggatatgg aaataaaatt
     7861 gcaggaatgg aaggattctc tcgggtttta tggggagcgg ggcccgcaat tgaccaatta
     7921 gatgaacgat ggttgaaatt aattctatca ggaataaaag ctggaacaga tccttcccat
     7981 cccgattatt ggggagaaat acatgaaaga gaccagcgta tggtagaaat gcccgcaatg
     8041 ttattagcgt tatatcatca tgaacaatta ttatggaaaa aattagatac aggagaaaaa
     8101 agccagatta ttcattggtt ctcgcaaatt tttgaatatg aatgtgcaga tggtaattgg
     8161 caatttttta agataattgt cggaacaatt atccaaaaat ttggttatcc agttaaagaa
     8221 tcagcactga aagaagcatt tgataatata gaaaattgtt atcttcaaaa tggctggtat
     8281 cgagacagtt caagaggtcg ccaagattat tataatcctt ttgcatttca ttattatggt
     8341 ttgatttata gcgtactaga accagaaaag cccattagcc agatatataa gaaccgagct
     8401 attgattttt ctagagacta tattcatttt tttgcggaag atggtgcaaa tgtgcctttt
     8461 ggtcgaagca tgatttatcg ttttgcagtt tcaagctttt ggtcagctat gttgtgggcc
     8521 gatcttttgc cttttaaatt aggtgaaatc aaggggttag tcaatcggaa tataagatgg
     8581 tggatgcaaa aagaaatatt tgatgaaaaa ggtatcctga atattggtta tacctaccct
     8641 caattgacat tgactgaacc ctataatagt acgttatctc cattatggct gaataagatt
     8701 tttttattgt tggcgctacc tgaagaccat gagtattgga cggctaaaga aatgcctatg
     8761 cctattcgtg aaggaactaa acttctttca gaagccaatt tgctcataca acatgataat
     8821 ggacatacat tttttttgaa tgcgggacaa ttagggccaa atttccatac attgaccaat
     8881 gaaaaatatt taaaatttgc ttattctagt caatttggtt tttcgatacc gcgagcgaat
     8941 caattgaaag cagaaatggc aatggattca atgctaggga ttcagcgagc ggacacatat
     9001 atcacgacca gttttaagaa tgagagcatc aaaacctatg gacagttcat cgttcgaaat
     9061 aatgtacgag acattgtatg taagaaagag gtacttgcct ctacatggga acctacttta
     9121 caggcaaaaa tcagaacttg gcttattcct tttgaaggct ggcagattcg cgtacacaaa
     9181 gtagaattag atacagaata tgttctatat gagacagggt ttgctattga atgttctcct
     9241 gagaaagaag gaataatcat agaagaagac aaggaaaact atagggtgag tgaagctggc
     9301 ttttcaggta ttatctgttt aagtgacgat actattaaac gaaaatccac agctattatg
     9361 ggtttgccca atacaaactt gatgacatgg gaaaacactt ttattcctgg attagagggt
     9421 acatttggaa aaggaaccca ttggctcgga acaggagtgg ttgctcatca agatcgggat
     9481 tatagtttag aagtatggaa caatcgtccc aaaattgatt ttgatgggac aaccgggcta
     9541 actatccaaa ataaaaacaa taaaaaaaga ctaaaattat gctaaaggct ggtggggaaa
     9601 tgcttgataa agtgatgttt gaagagataa tacatccaga aaaatggata aatgaatgtt
     9661 ataaagaatt aatggaaaaa gaatggaata atgagatcga gcgttgtcgc agacacacgg
     9721 agaggttaat caaacgtatc agtaacagtc agtatcaatt cccaagcgag tgtcctataa
     9781 aaggacgtta tccattaaga gacaatattg gttggacaga aggattttgg acaggaatta
     9841 attggttgtt atttgagaag acgggtgatc ctttatttca acaaacagct gaaaaagcag
     9901 atatagattt tatccacagg attgactgtc aaattatcac taatcatcac gatcttggtt
     9961 ttttgtattc tttatcaacc gtagcagatt ataaaataac gggaaatcga aaaatgaagg
    10021 aagcttcaat tgatgctgca gaaacattac ttcagcgttt ttgggagaag gcaggagtta
    10081 tacaagcttg gggagatcct agtgatcctt cacaacaagg tagaatgatc atggattgtt
    10141 gcatgaatct tccattattg tattgggcga gtgaagaaac tggaaatcaa aaatataaaa
    10201 aagctgctca ggctcatatt caaaatgcag cagattattt agtaagagaa gatggttcaa
    10261 cttatcatac attttatatg gatgtcaaag atggaaaacc aagatatgga agtacgcatc
    10321 aaggttatag tgataattcc tgttgggcta gaggccaagc atgggggatt tacggttttg
    10381 cattaagtta tcgttatttg aaagaacaaa aatattttga tttaagcaaa atattagcca
    10441 attattttat gaatcggttg ccagaagata ccatttgtta ttgggatttg atctttgatg
    10501 attctagcag agaagaaaaa gatacatcta gtatggtgat catggtttgt ggattattag
    10561 aattaataga atcaggtcta ttagacaaaa cagaaaagaa cttataccag tctgctgttt
    10621 ggaaaattat gcgacaattg aaagaaagat atgctacaac ggaagaagaa gatggctttt
    10681 taacacagtc tgtttataat aagcccaaaa ataatggggt aaatacacct tgtatttggg
    10741 gtgattacta ttatttagaa gctttagtga gaatgactag taattggtct tcttattggt
    10801 gaaaaaggta aatatggaat ttttgtttga taaaataaaa aaagaaaaac caaaacagca
    10861 tgaatggttt ctgttttggt tttttcgtta attttcatat tcttctaatt ccaagcttag
    10921 ttcttcccat tccgtcaatt ttgtttcttg atcttgtcta gcagcttcta aatcttggtt
    10981 cagctcattg agttttacgt gatcttccag gttttctggc gcgaccattt cttgttccaa
    11041 ctgatcgatc cattcatcga tccgcgccat ttcttcttca acctgagtaa tttttcgttg
    11101 aaggctacgg ataagctttt gctgttcttt gctttggtaa taatcagatt tgttcgtggg
    11161 agcaggagca gattctggct catcttgtgc caacaattcg gcgatttctt cttcttcctg
    11221 ctttttctct aaataataat catagtctcc tagatagact ttgctgccat tttcagagag
    11281 ttcgacgact tttgtcgcga tccgattgat gaaatatcgg tcatgggaaa caaaaagcag
    11341 tgtgccgtca taatcgatca atgcattttc taatacttct ttgttgtcaa tatctaaatg
    11401 gtttgttggt tcatccaaaa tcaaaaaatt ttctttgttc atcgctaact tggctaaagc
    11461 aacccgtgct ttttcaccgc cacttagcag agggattggt ttttctacat cttcgccaga
    11521 aaataagaaa gcacctaaag cacttcggat ctctttttct ggagtggtag gatgctcatc
    11581 ccacaattct gctaggatcg tcttgtttgt atgcagatca gcttgacctt gatcgtaata
    11641 gccgatggtg acatttgtcc ctagcgtttc ttttccacgg ataaaaggga tctggtgaat
    11701 gattgatttc agcaacgtcg atttcccgat accattcgga cctactagag caatcgcttc
    11761 ttgacgacgg atatctagat tgattggttc agataaggtg acattatcat atccgatagc
    11821 ggcatcttcg atttgcaaga ctacatttcc agaaggcttt tctatactga atagaaagtt
    11881 tgcttgtttt tcatcccctt gaggacgatc cagccggtcc attttttcta atgtctttct
    11941 gcggctttgc gctcgttttg tggtagaggc gcgaacaagg ttccgagcaa cgaagtcttc
    12001 taatttatgg atttctgttt gctgcttttc ataagccttc cattcacttg ctaactgttc
    12061 tgctttcaaa tctagatatt tcgaatagtt acctttgtaa tggcgcattt ttcttctgct
    12121 taattcatag acttcattga ctactttatc taaaaaataa cggtcatggg aaacaatcaa
    12181 taatgcacca gtatagcttt gcagataatt ctcaagccag gaaagtgtct cgatatctaa
    12241 atggttcgtt ggttcatcta gaatcaaaat gtcagggtgt tgcaaaagca tgcgagctaa
    12301 agctaaacgt gtgcgctgtc cgcctgataa agtatcgatt ttctgatcat aaaatgaaga
    12361 atcgaattta aacccatgca atacggaacg aatttcattt tcataaccat agccgtttcg
    12421 atctgaaaaa tcgtgttgca gccggtcgta ttcttttaaa taagaactat aagcaggtgt
    12481 gtctggcgta ccttcgctaa tgaaaatctc tagctcgcgc atccgctctt ccatttgtct
    12541 aacttgttca aaagctttca gcatctcgtt ccaaactgtt tcattggaat caagtcctgt
    12601 gtcctgagcg aggtatccga gagtcgctgt tttattttta gcgatctttc cttcatcagg
    12661 tgcttcaatt ccagcaatga tctttaataa agtcgatttt ccagcgccat ttctgccaac
    12721 taaagcgaca cgagcgcctg tactgatttc taaatgtatg ttttcaaata agacttctgc
    12781 accaaaatgg cgagcaatct gattggcttg taataatatc atggaatcct cttttctact
    12841 ttgtgaattc tcctcaaata aaaaactatt tttcaattag tagtctacca taaattccat
    12901 tatactgcga gatttgcaga aatgataaat aaatgaataa agaataaaat ttcacaatat
    12961 ctgagcaatg gaattgattt tttcctataa atactgctat gatggagaaa tcaaagatgt
    13021 aattagacaa tacgtggagg tcatttcgtg aaagatcata ttattccaaa ggcgacagct
    13081 aaaagactgc ctttgtatta tcgctattta aggattttaa ataatgctgg aaagaaaaaa
    13141 gtgtcttcaa ctgaattaag tgaagccgtt caagtagata gtgcaacgat ccgtcgagat
    13201 ttttcttatt ttggcgaatt aggaaaacgt gggtatggtt atgatgtaga agatcttatg
    13261 cacttttttg caaagacatt aaacgacgat caattgacaa atgttgcgct gatcggtgta
    13321 gggaacttgg gaagtgcttt actaaaatac aaattccatc aaagtaatag catacgtatc
    13381 agttgtgctt ttgatgtgaa tgaggaaatc gttgggcgca tcgtagacgg tatccccgtt
    13441 tatccaatgg aagatatgtc agaacaaatc aagatccaac aaatcgaagt agcaatttta
    13501 acgatacccg ctgaaagagc acaagaagta gtcaatcagt taacggaagc aggaatcaaa
    13561 ggaatcttga actttaccgc tgctcgattg aatgctccgt cagatgtatt gatccaaaat
    13621 gttgatttga cgaatgaatt gcaaaccttg atctatttct tgcattctga cgcaaatgcg
    13681 cattccgaaa atacagcaga ataaaaataa aaagcgattg aacaaatgaa ggcaaaactt
    13741 tcacttgttc aatcgctttt ttctgtttgg acaacgatta ttttgttcgg aagatttctt
    13801 cataagaaag ggaatcatct tctgctgtca gttcatctag tagataacgg atttttttaa
    13861 agtcgatgaa ccgtgcttgc cgaaagactt cttttccttg atgaaaaatc aggacaactg
    13921 gcgcagtcag cacttcaaat ttactagcta cttctggtgt ttcaaatgca tccaaatgaa
    13981 gtgccggaat atcataatgt gtgagaagtt cctctagtcg tggacgaaca gcgtgacaaa
    14041 cactgcaatt aggcatcgaa agataaatga cggcaacgga tgactgttgc agtatgtctt
    14101 gaacttcagt caagttagtg gtttgtttca tttttattct cccttttatg atagaagtga
    14161 tatgtatgag accttaattt aatgggaata acagaaaaag tcaattgaag tgctttggta
    14221 aaaaataaga gttgtgtaaa aatttacaga gcaacttgtt ttttttgaat aaaagcgtat
    14281 atttgcaaag cgaatgaact agaaataaaa aggaggaaac tcatgttttc ttttattcca
    14341 aattcggttt tgcagatgag tacgattttc ttatcgatcg ttattgaagc attacctttc
    14401 gtattattag gctgtcttat ctcaggcgcc ttacaagtat ttttgacccc agaacgtgtg
    14461 aagagatggc ttcctgaaaa tcgtatcttc tctattttta ctggttcgat cttaggattt
    14521 ttctttcctt catgtgaatg cggcatcgta ccgatcgttc atcaatttgt taaaaaaggc
    14581 gtaccagttc acacagcgtt tgcttttatg ctgactgctc cgatcatcaa tccaatcgtg
    14641 attttttcca cttttatcgc atttggcaat tcttggcaga tggcaagctg gcgggtgata
    14701 ggcagctttt ttgttgccct tgtgattggt gtctggcttg cctattttca aaaagacgct
    14761 gtattgaaag aaaaaatcac gtctaaagta gcagaaaatc accatcatca tgtccatgaa
    14821 gaagaacaaa gtgagaagaa tgaaccaaaa aatcagctga tccatcaaac tgggcatgtg
    14881 ttgacacatt caatcgacga attttttgat acaggtcgtt atttgatcat tggcggatta
    14941 atcgcagctg cgatgcagac ttatttacca actagaatta tgctaacgct cggctcaaca
    15001 aagattttag ccattatcat catgctgtta ttggccttta cgatgtctct ttgctcagaa
    15061 gcagatgcgt ttataggatc ttctttgtta agtctgttcg gcacagcacc tgtagctgcc
    15121 tttttggtct ttggcccaat ggtcgatatc aaaaatctac tgatgatgaa acgatatttt
    15181 acaggacgct ttattttatc gctgatcgga ctgattgccc ttagcgttat tggattcaca
    15241 ttatttattt aggagggaaa atatgctgcg gtttttgatt ttatctgggt atttcgaatt
    15301 gatgatgtac ttgcaagttt ctggcaaact cgatcaatat atcaatgtcc attatcgcta
    15361 cttagctatt ctttcaatga ttttatctgc tcttttggct ttagttcagc tgtatttgtg
    15421 ggtcaaaaac ggaaaagcag aagaaaaggc acacgagcac gatcatgacc atgggttgtc
    15481 aaaaccttat caacgtggaa tcgcttatgt actgctagct ttgccagtaa tcgtggggac
    15541 gttgtttcca acagttagtc tcgacactac gatcgtagaa gccaaaggat ttaatttccc
    15601 agtaagtaaa gaatcagtag gggatccgga gatgcagaca caatacttaa agccggatac
    15661 cagtatttat ttcaacaaat cagactactt agaccaaatg aatgagttga aagaaaagta
    15721 tggggataag caaactatca agatcacaga tgaaaactat ttagaagtta tggagctaat
    15781 ttataattac cctagcgaat tcattggcaa aaaaatcagc tatgaaggat ttgtttacca
    15841 aacaccgaac gatagcaata cagatatctt cttgttccga ttcggcatta tccattgtgt
    15901 ggcagattca ggagtctttg gtttgttgac gcatatgccg gaaggagtaa cggttaaaaa
    15961 tgatgaatgg tataaaatag aaggaaccat ccaatcagac tattatcaac cattcaagcg
    16021 tgaaatccca tctgtagttg ttacaaacgc tgagaaagtc tctgctccga aaaatcagta
    16081 tgtgtatcgt tctttttaaa agaacaaaat aaataaacat agctcagaca gctgacacgc
    16141 aaggacgtcg gttgtctggc tttttttgca tgatgtattc atttgtttga tatttatttt
    16201 agaagcagca tcctgaaagt atttgatatc ggtttcaaag gtgttggaaa agattgtgaa
    16261 gagaattaca atttattcct aacatgtatg ttgaaaagtt gtatgaaatg attttgatag
    16321 taaaatatcg agtggaggta ctttttatgg taacgcgtat atttattttg gatgatagtt
    16381 tggtttttga aaaaatgcta gtagaaattt tggaagaatc cagaatttta ttaagtcctt
    16441 ggaaatttga gatcgttcag ggattgaacg ttatacaatt tgttgaattc attaaaaata
    16501 atgaaatcta ttcctcagat atttttttcc tagacatcga tttgaacgcc tattatagcg
    16561 gtatcgaact aggcgaaatg cttagagaaa agaatgaaaa gtgtaaaatt gtttatctaa
    16621 cgtctcatcc gaataaagca ataactgtta ttaatcgaaa gattacgcct agtggttatt
    16681 tgataaaaga attcacttat ggtgttagta tcaaatctta gacagctttt cgtagagaca
    16741 agaataaatt aaacttatct acgagaggat gaatttttat gacccgatat gaagaaaatt
    16801 ttaaacaaat gattgttgaa ctgaatcaaa ctggacgttc tgttcgaggg ttagcgaaag
    16861 aatatggctt atctgaagca acgatttaca aatggaagaa tttatattta cctgatcagt
    16921 ccacaggact gactggaaaa gaagtagctg aactgagaaa agaaaatgct cgtttaaatg
    16981 aggaacttga aatcttaaaa aaagccgcag ccatattctc tcggaaaacc taaattcgct
    17041 tgttcaattt attgaaaaat ggtgtaagga ttacactgtt tctttgttat gtcggttatt
    17101 agaaatccct agaagtgtct attattttta taagaataaa ccgttgacag ctacagaaat
    17161 cagaaataat aagttgaaaa agaagatttc aacaattttt tttacgaata aacaacgcta
    17221 tggtgccaca aagatccatc aagttttatt aaaagaaggg atttcagtat ctcttaaaca
    17281 tgtccaaaag ctaatgaaac aattaaattt aaggtcgatt gtagttaaaa aatatagacc
    17341 tcaaaggtct aataaaccga tcatttcaaa agagaatctc ttaaatcagg acttttctac
    17401 tgaaacgatc tgtgagaaat gggcagctga cattacgtac attcctacta agaaaaatgg
    17461 ttggtgttac ttatcttcaa tcatggattt acacacgaaa aaaattatta gttatacatt
    17521 ttcaaaacga atgactgtgg attgtgtcat tcaaacattg aataaagcaa aaatacatta
    17581 tcacattcca gaaggaatga ttctacacac tgacttgggc agccaataca cagcaagaga
    17641 agtggaacaa tggcttaaaa ccaacaaaat aaggcattct tatagtagaa aaggaacacc
    17701 ttatgataat gctggaatcg aatctttcca tgcctcattg aaaaaagaag aagtctacac
    17761 gactagctac tcagattttg aagaagcaaa tcgagcgcta tttagctaca ttgaaggatt
    17821 ttataaccga aatcgaatcc atagctcgat tcactatcta accccacagg aatttgaaga
    17881 attagcaaaa gaaaaaatgg cttaaccgtc tctactaaaa tgtgtccaag atattgactc
    17941 aaatccactt atgaaaaaaa taaaaaaagc atccaaagtt acttattgaa agaaaaagaa
    18001 aactatcctg ttcaacatgc tacgaaaaaa aggtggacaa ctgtccgtta tggtaatagc
    18061 gatcactata tccattacga tgaaatcctt tatctaatga gcatgccagg ttataaaaat
    18121 aaaataggcc tagtattgaa agatgaagag attctcgtaa acggcacaat taaaaaatat
    18181 aaaaaatcac tcgaatatga gtatttatgt aaagatttaa aagcttatat cattaatact
    18241 gaaaacatta catctattag cagagtaaac gaaatagtaa catttgcaaa tggagaacaa
    18301 ctagagatgg gaaagagaag tattgataaa ctaaagaatt ttatgcaaga aaagatagaa
    18361 aatgaatcat tttaaagaaa acaaagactc atatcttacc tgttagaaga gtgttttcct
    18421 gtagaagaga acaacccaag tagcagccta gtcgtttcta tccttttttg ttatgcactc
    18481 tttttgacat ttactccacc aataacgaag ggtacaataa aaagtaataa caaacaaaag
    18541 gagtacagaa atgattgaat tcatcaatgt aagtaaaagt tacgggaaaa aacaagcatt
    18601 aaaagacttg tctctctcga taagacaagg ggaaattttt ggatttttag gacataatgg
    18661 tgccggaaaa tcaactacga ttaaaagttt agttagtatt attgaaccat catcgggaac
    18721 gattttagta gatggaatga aattgacgga aaatcgattg agtatcaaac aaaaaatcgg
    18781 ttatgtgcct gattccccag atattttttt acaattgacg gctggagaat attgggactt
    18841 gatttcagct gcctatgaat tagacacaca aaaaaaagaa aagcgtttag cagaattgac
    18901 agcgcttttt gatatgtata gtcatcaaaa tgaaacaatc gcaagttttt cacatggaat
    18961 gcgtcaaaaa acgattttga tcggcacgtt gctgccagat ccagatattt gggtattgga
    19021 tgaacctttg caaggacttg atcctcaagc agcatttgat ttgaaagaaa tgatgaaggc
    19081 tcatgccgca aaaggaaaaa cagtcatttt ttctacacat gtattggata ccgcacaaca
    19141 gttatgcgat aaactggcta ttttgaagaa aggtgaattg atttatcagg gggcagttag
    19201 tgaattattg aatggctcac ctgatgaaac gctagaaaag atttatttga aaatggctgg
    19261 aaggcaagcg acaggagatg gaccgtatgt ataagcgaca attattagaa ttaattcgag
    19321 ttaatctttt atatgttaat ccacagctca cgaataaagc aagagagaaa ggaaaaaagg
    19381 gaaagcgatt aactatctat cttttgtctc aaaatctttt ttcagggctg atttttttac
    19441 tgatttacgg atttgctatg tttgctatga actttgttaa attgccaggt ttctttactt
    19501 attacgtggc actttttggt atgttaggtt taactcaaag tatatcagtc atttataata
    19561 tcttttttga aggaaatgac ctgcaagtat ttttgcctct gccttttgga caagaacagg
    19621 ttttttcagc aaagattttg gtagttgctt tgaccatcat tccttttgtt tttccgcttc
    19681 tagtcatgtt tattttaacc ggatggcgag ctgatctagg gattttgctt actatcatta
    19741 tctcaatcct tttgttttct ctatttttga ttattctttt ttgtctggct agtttgattg
    19801 tttttggttt ggctcgaaca gcagtgttta aaaaacatag aaaaacagtt tctagtatct
    19861 tgcttggcag ctcgatgctg atcgcatttg ctggaatcat gtggatgaat catcaaacgg
    19921 ggactattga aaatgagtta acagatcggc atccgatttc gatattattg ccattctatg
    19981 atgctgccag tcagccactt tctcaacaag gtttattggg ttggggaata ctagctatca
    20041 cggctctggg atttttcctt gctgtaaaac agctgatagt accaaagctt acagagcaac
    20101 tgactgattc aaacagtgag ttcaaaataa aaagaaccca taaaaagaac caaaatgtgc
    20161 atcagctttt atttagttac aatgctcaat tagtaaaaga acctaatttg attatgcaag
    20221 ttctttcttc ttccttgtta actccaatca tttttattct tgcttttgcc ctaggaggcg
    20281 aaataaaatt aactgattta gataataagt taatcggtgt tgttttcctg gtagggatcg
    20341 cattggcttt cttaacagtt aatcagactt cttttatcag caatcttatt tctttggatc
    20401 aagaaaattt tctgtttatc cgttctttgc caatctctat gaaccaatat ttaaaagaaa
    20461 agttcagatt cggatggata ttgcagagtt ttttaactgg tgggatcgca ttattaagcg
    20521 gactgtcttt tcagctgcct ttgtttttta tccctagctt gctatttggt tctttattcg
    20581 gctgctactt attgtcacta cgttatttcg ctagagatta tcggttgctg ctgctgaact
    20641 ggaccaatat taaccaatta tttaacagag gttcaggaag tttgggttta gtcgcaacta
    20701 tgatgggttc attgatcatc agtattattc ttatcgtctt atacggaatg ttggctttgt
    20761 tttatccatt ttggttagtg aatattcctg tgatcggttt agttctaata ctttccgctc
    20821 tatggatcgt ttattatcaa cgaacgtttt ggaaaaaatt tgaataaaga agaggggccg
    20881 tgacaaaaat catgtcgcag cccctttttt acagatagag ggtgttcaaa agtcagtttc
    20941 tcagtattaa gtcaatttta tgtaaaaatg tgagaagcta tttttacaaa acttttttta
    21001 atactcataa ttacttctta ttgctcctgc ggttacttgt cgcaatgttt gtgagatatt
    21061 ttgtcatcgc tcaaggctga acgtctcttt cacaaccttt tctttgttac acttcgttta
    21121 tttaaaaagc cgtttttttc gtttcatcat gattgcgtca cgctttactt tgaaattacg
    21181 gatgttatgg ttaaagactt ctgataatac aagacctaaa gcgattgctc cagcaatcat
    21241 aattgcttgg accgcataat gagccgcttc tcgataggat tcagtaacga gatttcgaac
    21301 cgcttgataa gctaaaccac cagggactag aggaaccatc ccgggaatat tgaaaatcgt
    21361 tacgggcatt ttcaaacgtt tagagaaaag atcgctgatg aatgctactc ctaaagaacc
    21421 aagcaatgaa ccaaaagcag cagatccgcc tatttgaaca cagatccagt agatcatcca
    21481 gccaaaagca ccagaaagac cacaacaaat caatgaacgc ctaggtacat ttgtaataac
    21541 ggcaaatgcc gcagtcgcta aaaaactaaa agaaaactga ataaatatcg tccccatact
    21601 tgtgttcctt tcctaataaa aaagctggaa aataaaagca atcgcaaaac cgatcatcgt
    21661 agcagtcatc atcgcttctg ttccgcgaga aactcctgag acataatgtc cagctagcaa
    21721 atcacggata gcgttcgtaa tttgaactcc aggaactaaa ggcatcacac atccgataat
    21781 gattagatcc tgattgcttc ctaagccaaa acgtacgctg tagatagcgg ctatgccaat
    21841 caataaagaa gataaaaatt ccgataaaaa tttgattcta aaaaatttca aactatatag
    21901 ataagctgcg tatcccaatc ctccaatgac taaagtcaca ggtaaatcgg tcatctttcc
    21961 gccaaataac accataattg ttccactgat cactgccgcg ctaacgattt gcaaccagac
    22021 aggaaagaaa gaacggtctc ggtcgattgc ttgcagttcg cagtaaagtt cttctaatgt
    22081 tagctctgaa gcaacatatt tacgtgaaag atcatttact tttactactt tttctaaatt
    22141 aatcgaacgg ttggtaattt gttccattcg gatggtcgag gtacgatcaa gaccaataaa
    22201 gagaccggtt tgagtgacat agctgaccaa acggaaattt ccagaagcta aagcaattcg
    22261 actcatcgta tcttctaccc gatacatctc cgcattgctt tccatcatga ttttcccagc
    22321 gagtaaacaa gtgtccagca acagatctaa ttcttttccc gtcatagcat cttctccaat
    22381 aattgattga ttagttttat tttactacaa aacataccag aagcgtaaag aataaaaaaa
    22441 cttgaggaag gaataaaaga ggcgtatact gtttttgtat tgcgggtgta gtttagtggt
    22501 aaaatcccag cttcccaagc tgatgtcgcg ggttcgattc ccgtcacccg cttaaaatag
    22561 gttgtgaaaa agctgctctg caacaagtaa taagaacagc caagcaaaaa caacttctta
    22621 tgtttttaac ttggatggac ttattaccgc agatgcaagc ttttgcgcac cgtttaccaa
    22681 gcggttgtga aatcagtgta ttgtcttgag tatgaagaaa atttttgaat aataataaat
    22741 accttacaga tagagtggtg actgattaga gacgaggaat ccgtctcttt tttgtatttt
    22801 taagccatct taaaacaggt tttatttttt gctgtttcta tttctttcta gtatttattt
    22861 ataggggaac atatgttaag atttagggag ctgttagtaa aggagtaaag aaatgagcaa
    22921 aaaaacaacc aaggcgatcg tggggatcgt cgctgctgtg attgcagggg cattcggtgt
    22981 ttccaccaca caaaaagatt cagatatctt tcagcagatt acaactattt ttgattccac
    23041 accacaagct tctcaagtag cggatgtgcc agaatatgat ggaaaacatc aagaaataga
    23101 aatcaatggg aatcagccgg aatttacaaa agaggatctt agcctttcca aaggaacatg
    23161 ggaaagctat tcaaatattg ataaattgaa cagagtagga caagccaatg ctatgcttgg
    23221 aaaagatttg ttccctactg aaaagagaga gcctttgtat attgacccga caggttggaa
    23281 acaaaagaaa ttaagtgacg ggcaatggtt atataatcgc agccatttaa ttggtttcca
    23341 gctgactggt caaaataaca acattaaaaa cttgatgaca gggacacgtt cgttgaatgc
    23401 tccatacatg cttgctcacg aaaatgatat tgcggcatat atcaaagaaa caaatcatca
    23461 tgtacgctac cgtgtcactc ctcattttga ggggaatgaa ctggtagcca ggggagtgca
    23521 attggaagca gaaagcattg aagacaaaaa aattgaattc aatgtcttta tctacaatgt
    23581 tcaagatggc tacacgatca attatgagac aggacaagca acaaaaacaa aaaattagta
    23641 gcagctattc gaatttttta tgaatattat gaaaaaattg tgtaaaaaca tacattcaaa
    23701 gaaatatagc gtatacttta tgaaaagagt acttttgata aagctacaga aaaaaggaaa
    23761 aaaacataca aatagtgttt cttaatttat tcggaagttt ggaatatttg tttgtagttt
    23821 tccctttctt cgagtaaaat aaggttgtag aaggtaagct ggaattacct tgcgggaaag
    23881 aggtgtagaa tcatgggttt tacagatgaa accgtacgtt ttggttttga cgatagtcgc
    23941 aaaaaagaag tgagcgaaac attagcgatt gtttatcggg ctttggaaga aaaaggctac
    24001 aatccaatca atcagattgt cggctacttg ctttctggag acccgtccta cattcctcgt
    24061 tatcgtgatg ctcgaaattt gattcgccgt cacgaacgcg atgagatcat ggaagtaatt
    24121 gtcaaggact acctctcaaa tcatggagtg aatttatgag agtaatgggc ttggatgtcg
    24181 gttctaagac agtaggtgtt gctatcagtg acccctttgg ctggacagct caaggagtag
    24241 agatcattcg gatcaatgaa gcagaagaag agtttggctt tgatcggttg aaggaattag
    24301 tagcacaata tgaagtagac cggtttgtag ttggattacc taaaaatatg aacaattcca
    24361 tcggaccaag ggcagaagct tccatgtcat acggagaaaa gatcaaagaa ctattctcct
    24421 tgccagttga ttatcaagat gaacgattaa ccactgttca ggcagaacgt atgctagtag
    24481 agcaggcaaa tacttcaaga gccaaacgaa aaaaagtcat cgataaatta gcagctgtga
    24541 tgatcttaca aaattattta gacggctcag gtaagcaatt attttaataa taaaagaaaa
    24601 gaggtaatct tatgacagaa cataaccatt cccacgacca tgaaggacac gaacacatca
    24661 cattggtgga tgaccaagga aatgaaacat tatatgaaat cctactaaca gttgacggac
    24721 aagaagaatt tggacgtaac tatgttcttt tatatcctgc tggtgtttca gaagatgaag
    24781 atgtagaatt acaagcttat gcatacatcg aaaacgaaga tggaacagaa ggcgagttag
    24841 aacaaatcga aacagacgca gaatgggaca tgattgaaga agtattcaat acattcatgg
    24901 cggaagaaga agaatagact ataaagctag gagaaacctt gatatgatag ggtttctcct
    24961 ttttttattt tttactgtat gccatattgt atgccatcta gtcaatattt aagtgttgta
    25021 ctaatttttc aacagtcttt tctttcgttt cttttgccaa gtgaccgtaa caatcgatcg
    25081 tctgactaat ttttgtatgc cgtaatctag tttgaacttc taccatagta gctccagcgt
    25141 ccaagagcat tgaagcatga gtgtgtcttg ctacatgcac accgagaaat ggaaccatag
    25201 ctttttcaca aatagtaatc aatcttgcat aaatagcgtg gtgtgtgatt agttctttat
    25261 agataccaca aaaaatcata gtcggatcag ttacaccaag tgtcagcata tatttccgct
    25321 gattaatctg ccattttttt aatatagaaa ttgttctttc atctagtgga agaaccccag
    25381 cactacaatc agttttagga tcactgattt tatagccatt tttggattgg cttagtgttt
    25441 tattgatggt gaccgtattt tcttgaaaat caatgtctga ccaattcaat gcaagggctt
    25501 cactgatcct aaccccgcta taaaagagaa agcgtagtaa cgttttatca aagtcgtttc
    25561 gataagtttc aggtttgctg tttaggtaat caaacaaggt gatcagttgt tctttactgt
    25621 aaactttgac cttttcttta tgcttagtgg tggttctttt aggcattaat acgttgtcta
    25681 gtggattagt ctcaataatc ccaagattga tagcgtagct aaaaatacgc ttagaagttg
    25741 aatgaagtac cttgtattgg tcagtagttt cagcccagct atttacaatt tgttgggctt
    25801 tttttaccgt gacttcattc acacgaagat ttccatattt tggaagaatc cactttctaa
    25861 acttgatttc agttttcatg aaggtggctt ctcggacagt ggttttatat gtttctagcc
    25921 acaagtcata aacttcttta aacgtttcgt tgttcgcttt tttaataccg tttttatcgt
    25981 agtccacttg caagcgtgac aaggcaagct gggcttcttt ttttgttttg taacctcgtc
    26041 tacgaacctt atcaggtttg ccagtcattt tgttcattcc aagataggct tcatagagcc
    26101 aaaatttacc ttttttagtt tcatattgct tgaatgttgc cataatagtt ttccctccat
    26161 agtgtagcaa gccgtggggg cggttggagg aggcagttta ttattatttt gaatagtgtt
    26221 tatttagctc agtaacaaga gctgtgaact cttttagaag atcattttca taaaattctt
    26281 gatactcttt tttctcatct gtattttttt ctgaatcatt aattaatgag tcatatcgta
    26341 aaagcgtatt tagaagagct gcgagataag caattggggt actcccgtca ggaaattcaa
    26401 ttcgagatcc tgttttaaga tattttattg cgtttgttaa ataaatcttc tgtgagacag
    26461 tcaggttatc ttcttcgatg ttattgatag cattagaaag attagaatca attatagagt
    26521 tatttttttc aatagttttt ttcaattgct tttcaagcac atttcgctta atttcgggca
    26581 tcatatgcat atctcttaat gtataagatc cgtttaatag ataggtagtg gatacatcta
    26641 atacctttga aagttgttta agcctggtgt tattgggaag attatagcca ttttcccaat
    26701 tactaacggc acctttactg gccggtgggg ataatttacc tccaagttgt tccattgaga
    26761 gtcccttttc aattctagcc agtctaattc tttgtcctgt ctccactgct tcggggtatt
    26821 tttttctagc cacattttca ccaccattca tatattttct ctactatatc acgatttaaa
    26881 tatgtgtgaa acataaaagt tcaaaaaagt atgttaaaat tcttgcaaat gaaaaggtat
    26941 gatggtatat tgagtttata agaggtatag aaatgtattc aagagcctct tgcatttttt
    27001 taaaatcttt gtatagaaat gtatgctaac gaggggggat aaatatggaa ttaaaaattg
    27061 acaaactcac gcttgatgat ccagctttta aaattattga cgatcgagtt gttcaacaag
    27121 tagagcaaac tcaaggaacg ttactttcat tattagaaaa aaagtaccag tttccaaaat
    27181 atatgaacaa ggcacaagct gctaagtata tgaacgtttc atacaatact atgatgcaga
    27241 aatatgttcc taacggttta aagttgatta tagttgatgg agtcgtaaga atttcacaag
    27301 atgaatgtga tcgctttatg gaagaatacc aaaaataaac gcaagccgtg ggggcaaagg
    27361 gattaatagg aggaaataat aatggaatcg acacaaaata aagcaatcga aaaagtatta
    27421 tcaacaattc ttacagaaga cacagcggca gaattagcaa acttggaagg caaagcgcta
    27481 gaagaaacgt ttgaatgttt gtacgaacaa atggactatc aaaaattgct accacaagaa
    27541 ccaacggtaa gcggagtatt gcgtggcttg aacgatttag tacaagcaga gtttaaagaa
    27601 cgactttcta ttgaagagta tcaagaaatt ttgtatcagc aagttgagtt gttagcgagc
    27661 ttgctagggc ttgaactgga ggacgttgaa tgaaaactga aatttggaac ggacacatta
    27721 taagatttgt cgatattaat gatgaatggt gggcagtggc gaaagatgtt gcggaagcgt
    27781 taggtcttaa acaggttact agagccattc actctttgcc taaagatggg gttactacta
    27841 gtaaggtcat cgattcattg ggcagaacac aagatgtaaa catcatcaac gaaaaaaaat
    27901 atttaccgca tggcattcaa aagccgtaaa aaagaagccg aggcatttca agactggatt
    27961 ttcgacatca tcaaagaact acgccagtcc acaggacttg aaggctttca agtattccgc
    28021 atgctagaca aagaacacca aaaagaagca atgactaaat taagccacgc tattactgaa
    28081 ccaaaaccag tagactacat taaagcgaat gtgatagcca ataaagctgt atcaactatt
    28141 tatggtcatt ccaaaatggt aaaaaagaaa gatatgactc ctgaaatgtt ggttgatcgt
    28201 gaacctattt tagatgagac agtagaatta atgactgtta aagaaaaata tgggttgcag
    28261 ttttcagtga gtgagaaaat ttataatcgt tctgctgaat tgcagacaac ttaggagcat
    28321 taacatgatc gagaacaaag aaaaagcctt atccataaca gtcttggcgg acaggataaa
    28381 ggcaacttaa ttgggataaa acccgatatt ctttgttccg attataccat attaaaaaat
    28441 cggagggaac cattatgaaa cgaaacttaa ttataggaag cattttaatc ggcggaattt
    28501 tcggcttatt accattaatt gcaaaaatag ctatctttgc aggattagcg ttatatctct
    28561 tcactgctta tgacgagtac gactatcaat taagaactgg agggcataag tagatgtaca
    28621 aaacaaaact attaaaccaa ctcgatagcc ttgaattaga ggaaattaat caagggatcg
    28681 ctgaactaga aaataatatc gggaaaacct actttggaaa ttcattcaat gaaaaactaa
    28741 cagttttgta tgtactcaaa aaacatgcag aacacaaaat aatttgtcga gaaatcaacg
    28801 agctaaaaaa tcagatttta accgcatggt taaacattac tgatatgcaa gaagcaaggg
    28861 tgaaaacatt taatacatgg gtaaagtatc agaatcaatt aaaaggtgct gaatttgttc
    28921 gggatgggtt gaaatacgaa cttgagcagt tgaagctcat ggaggtgtct gaatgattta
    28981 taccagtatt ttgaaaaagg aggagaggtt tattggcaaa taattatcaa gcagcaacgg
    29041 aattacaaaa gtcgggatgg tcaatttatc ccttatctcc tggaactaaa attccaataa
    29101 aaggctctag tggttggaat gatgcaacta acgattctaa tcaaattaat gaatggtgga
    29161 cggaagatgc aactagaaat attgggttaa tgcttgaaga tcatcaaatg gtggtagttg
    29221 acctagatca acattctgaa acacgaaacg gcgttgagaa ttataaaaaa cttgcttcaa
    29281 tgtatgatcc atttccttct acttatacgg aaatcacacc aggtaaagga atccatttct
    29341 tctttaaaat tcctgatggt gtaattatcc aacaagaaac aggtgcattt gctgatatct
    29401 tcgggcgtga tgataaaggt aaaagtttaa gcgggattga tattatcact aaaggagttc
    29461 caatagcgcc aactatgatt atttcaagaa gtgttgggag agcatatgaa cctattgaaa
    29521 acgtatctct aaataatatt gcaccagctc ccgattggct agtgaaccta ctgctaaaaa
    29581 aaccgcaaga aataaagact caataccgac ccgtagcaaa aacagggaca gcaaaaaaat
    29641 tagatatgat tgttcaaggc gctggcgaag gtagtagaaa cgatcattta actaagttat
    29701 gcggttggtt actatggcac ggtgtagata atcaaacttt agcagaattg atttatacag
    29761 caaatgcgta caatgaacca ccgctaaaag ataaagaagt gaatcaaatt attcgatcga
    29821 tgattaaaaa agatttgagg gggaaagctc atggctcata agcataaatt agatagtatt
    29881 ttagactttc ccgaagccag cgagcgtgaa gacaatatca ttgaattgaa aacctggatg
    29941 tcacgattaa gatgcaataa agacgatcag attaaaagta acagtgttgt gaatgcagaa
    30001 ttgattttaa ccaatgatag caatttagct ggaacaattg cttataacga atttagcgga
    30061 tacattcacc tattaaaaga ttctccttgg atcaatcgta ccgctggaga atgggaagac
    30121 agttttgagg atgccttaac ggcatatatt gaagaaaact acaatgtagt ttttgatgac
    30181 aacaaaatac acaaagcagt agtaaatgtg gctagaaaga atgttttcaa cccagtaaaa
    30241 gaacgtatcg aaaaagttaa atgggatcaa cagcctagac tggaaacact gtttattgac
    30301 cttttagggg tagacgacaa cctttacaca cgagaagtaa caaaacgttg gatagtcgga
    30361 tcagtcgcac gtatctataa accaggaata aagtttgaga tcgttccagt tttggatgga
    30421 ccgcaaggga ttggtaagag taccgttcca gcattacttt acacggatga ttttttcacc
    30481 gattcattag attcgctagg tgaaaagaaa gatgattata tgcagcttca aggcaatgta
    30541 attattgaac ttggagaatt atcttcaatg aacaaaacta aaattgaaca gataaaaaac
    30601 ttcatcagtg ccaagatcga taaaattaga cctccttatg gtcggaatgt catagcttgg
    30661 gcaaggcaat gtgtatttat tggaacgtcg aatgatgggc agtatttgaa agatgataca
    30721 ggaaaccgcc gtttttatcc tttgccttgt aagaataagc caaagatgaa tccgtttaaa
    30781 acaaaggatg aatattttct tcaagtgtta gctgaagcaa aggtgctttt tgataaagga
    30841 caacgaatct attttagtcc cgatgaagat aaagaagtct tagaaattgc aaaagactat
    30901 caagaggatg ccaagatgga gaatccaatt aaagaagcaa tcactaaata tttggaaatg
    30961 gaaatcccgt ataaatggga agaagctcca gcgtgggcga gacgttctta ttatcagaac
    31021 tatcctgaca gtaaatgtaa tgagaaaaat ttacagcatt tctataatgg agctactatg
    31081 caacaagagt tttatttagt tgatagcgta ctcaccgctg atattttgga agctgttttt
    31141 gataaacaag caaaggattt attgaatggt cgatcagatg cagaaactaa aaagattgcc
    31201 ttgattatta gcagtattcc tggttgggaa cggaagcaac tgccaaaaag aaataaacga
    31261 cgaggatttt acaataagtc aaatgcctat gaaaataaaa atcgagctgg aaagtacaaa
    31321 aagtaacggg tagtaacggg tcagtaacac ctagcccgtt actagtttac ccttactgcc
    31381 ccaagggata cagcaatttg gtaacggggt aacaccttat ttccttaaaa cttttgaaat
    31441 agttagctaa tatgctgggg aataatgtta ctggatttta cccgttacct gttaccaaaa
    31501 cgctagaacc cttgtccccg ttgggattga gtggtaacgg gtacctgtta cgtaggtgtt
    31561 accacgtgtt acgaatcaag gaggaaagta aatgctcaat attatttcaa caaacaaagc
    31621 accgaacttt caatatacag atgaaatgga tcggtttcta atgaacacgc tggcatttag
    31681 tgtgggattg gtaacagaag attattccac atttgatcca gaagtattaa agataatgga
    31741 agaagaacct gattggctgc aggagtcagt agcgtggtgt caatcgctag tagtgggatc
    31801 attggtagat agtggcaact atgatgatac aggtgagcta atggatgaat ttaattgtct
    31861 gcttaaccta tatgatcgag cgagacaacg agagcttaca tcgaatgagg ataatttgtt
    31921 tttaaacatc catgataagt tcttggcttt gctactaaca gatgatgaat tgataactaa
    31981 tttattggag gtagaataat tatgcatatg gaaagtatta gatacttcga tggtgagaaa
    32041 cacattgagt attcaaacac tcaagaagat gtagacttta ttagttttga ggaagataaa
    32101 atcactaaag ttaattttaa agacggaacg tgcttgaaga tagtttcgcc ttatattgaa
    32161 tacaaatcaa aatgggaaca agatgattct gacgaacgag atcgctttgg ttggtaatct
    32221 atggcagtta aaaaacaatg taatcatgct ggatgtaaaa cattgattga ttataggcag
    32281 aagtattgtg ggaagcataa agcaaagcaa acggcaatta agcgtgaaga aagaaagcaa
    32341 agcgaaggta aatattttca attctatcaa agtagaacat ggcgaaaggc ttcatacttg
    32401 tatcggttaa atcatccagt atgtgaggat tgcctagaag aaggcttgat aaggaaagct
    32461 gatgttgtag atcacaaaat tgaactaaaa gatgattgga atagacggtt agatgaaact
    32521 aattttcgtt ctttatgcca tgcacaccat aattccaaga cagccaatga aagacaaaga
    32581 cgagaaaaga gtaccctttg agttaagggg gcatagtgtg aaacttactg acaatcgatg
    32641 cctactcatt ttggtacaaa taaccgttgt gaaaaggcat aagggtaatt acaaataacg
    32701 attacacttg taatcacaag tgaaaaagta tataatgaaa gtaggagatt tattgaaaaa
    32761 tacaattgtt attactgata aaacagggaa acagcgcacc attgagttac ctcattttgg
    32821 tcaagtgacc attcaaatgc agaacggaaa aatcatttat atagataaat tagataaaga
    32881 aaaattctga tcgaaaaacg aaggaatgtg agcttaattg cttacgttcc tttttctttt
    32941 gtctgaaagg aggacaacat gggacaccct aaaatattac acgatacaaa aggcaatata
    33001 tcaagtgaag aaaaagcaat ccgtgtggac gctagagaag aattgttcaa gcagcaacca
    33061 ttaataaata tcacgccccc tgactggatg gcagcgagcg ctagaagtga atggaatcgt
    33121 atcgtaccaa cattaaaaaa agattatcca ttgagtgaag cagattatgg ttcattggta
    33181 gcctattgtt tagcctttgc tcgaatgaaa acagccgaag ccgagataag aaaatcaggt
    33241 acgtttatca catgcgaaaa cggagtaaag aaagccaatc cagcggttcg ggttcaatca
    33301 caagctatga gtgatttgaa aaaacaagcc acctcactag gtatgacctt agaatcacga
    33361 tcaaaactag ctttgaacaa ggttaaaaat gatgaacccg aagacccgtt caaagagttg
    33421 atgaattcat gaatgattac attgagaaag tcctatcagg taagctgatt gcacccaaga
    33481 aaattatcca agcgtgtgag cgtcatataa gcgatttgga gcgttctaag tcagatagct
    33541 tcccttatgt gttcgatgaa gaacaagcca ccaaagcgat taaattcatt gagttgctac
    33601 catctacgga cggtaaagca atcaagatgt taggatttca aaaattcatt ctaggcagtc
    33661 tttatggctg gcgtactaaa gaagggaatt acaggcgatt caatcgagcg tttaccagta
    33721 tgagccgtaa gaatgggaaa acgtatatcg caagtggcat ggctgccaat gcgttgatta
    33781 tggaacaaga accagcagaa gcaaggcaag tattgtttgt aagtaacgct ttgaaacaag
    33841 ctaaattggg ctatgatatg ctgtctaatt cacttagaaa cgtggtcaag tctagtaagt
    33901 ttttaagacc acaactgaaa attatgaact ctaagattca gcacttgccc tctaattcgt
    33961 tcgctatggc actggctagt gaaaccagca cgctagatgg gtttgcacca acaaccgcaa
    34021 ttcttgacga gtggcacgaa gcaaaaactc gtaaaacgta caacgtcatt aagtcaggaa
    34081 tgacccaaca aaagaacggc ttattgtgtg ttattagtac cgctgggctt gatttaaacg
    34141 ttcctatgta cgaggaatac ttattgttag atcgtgtgct aaaaggcgaa gaacaagccg
    34201 acaggtactt tatagcgata tgggaattgg acgatcccga agaaattcac gatcaagaga
    34261 aatggatcaa agccaatccg atttttgaaa gtgaagaaat caaaaaagta atgattccaa
    34321 ccattcaaga tgatgtgaac cttgctttga aacaaaataa ccttaattct gtattggtga
    34381 aaaacttcaa tttatggaga caagcgagtg aggacagcta tatgattgct gaagactggc
    34441 aggcaaccga agtagaacca caagatatta caggcaagcc cgtttatatc ggagtggatt
    34501 tatctaaaac agatgattta actagcgttt catggatcgt accgctagat aacggcgaac
    34561 tttattgtga ttcacatagc tttgtagcca ccaaatatgg gcttcaagac aaagaaaagc
    34621 gcgacggttt gccttatcga gaactagaaa aagctggtga gtgttccatt actcaattag
    34681 aaagtggaat cgtggactat gaccaagtat ttcagtttat tcaagatttg attcaagaaa
    34741 atgatttgga atgtatgggg atttgttatg acccgtataa cgctaattcg cttatcagta
    34801 aagctgaaaa agccaactac ccaatgttag aagtaagaca aggaacgatt actctaaacg
    34861 ttccgacccg aacttttaga gaacaagttt atgaaggcaa cgttattcac aataaaaata
    34921 cgattctcac ccatgcagtg aacaacgcta ttttaaaaac ggataacaac ggcattcaga
    34981 ttaataaatc aaagaacagt aacaaaattg atccaatagc tgcattaatc aatgcctatg
    35041 tgtttgcaat ggattacttc accacaacgg aaggagcgaa agcagacaat gaattttata
    35101 caagtgaaga attttctttc taattacatt cataccgttc ttttacttct tggattggtg
    35161 tgtgtgttgg ttgctatcac cttactaaca aatgtctact atggcttgtt agcgctgggc
    35221 atagtgctta ttggggtagc ggtcatgcta aatacagaac agaagggagg ttaaaagatg
    35281 gcatttttta aagcgagaca aaatacaacg ggagatcctt tcctacaaca tgtggtatca
    35341 atccaatcgg atgattacac caccagcttt acaagcgttc gtgcattaag aaatagtgat
    35401 gtgtttgcag ccgttcgaat tattgctagt gatattgctt caagtccgat tcaattggtc
    35461 aaaaacaata tgccacaagc tgataatgaa ctggtgaagt tactaaacga gaaacctaat
    35521 tcagaaatgg atggttggca tttaaagttt gctttggcgg tcaatatgtt gctaaacggt
    35581 aatagctttg cagagattaa acgcaacggt gaaaaggtag aagaaattca cttattacct
    35641 aactcaagtg taacggttac tcaattagat aatggtacgt tgtcttatca gattggtgat
    35701 aaaaaaagac gtgtgaagtc tagcgatatt ttgcacttca aatatttcac ccaagatggt
    35761 ttaacaggat taccaccact ttatgcgtta cgtgatgaac tgaaaattca acaagctggc
    35821 aaccgcacat tgcacaattt ctttactcgt ggtgtcagtg ggtcaggtat tttgaaggtt
    35881 cataagtcag atttagacgg atcagcaaag aacgcaattc gtgaaaagtt tgaagaagcc
    35941 aacggttcaa gtagtggaga taatgctctt agaacgatca ttcttgatga aacaatggac
    36001 tacaaaacat tagaaataaa caccgatgtt ttgaagctta tcaattccaa tgattggaac
    36061 acaaaacaaa ttgccaaggc gtttggtgta ccgattgagc gtttaggcgt tgaaaatgaa
    36121 cattctagca ccgttcaaag taatctccag tatatccaaa gtacgctgat tcattacttt
    36181 aatgtgtttg tgagtgaatt agatacgaaa cttgaaacca atatccgctt taattccgat
    36241 cagttactag agactgatcc tgaaacaaaa gtaaaaaatg tattggatca ggtcaaaggg
    36301 tcacttctca cgattaatga ggggcggtcg aaaatggggc taccccccat ggatggtggc
    36361 gatcgtttac tagcaagttt gaactttacg tatttagata cgttggagaa atatcaatta
    36421 aaagaacagg aaggagttac accagttgaa taatgaagaa gaaaaggaaa aacggctgac
    36481 agaagaagct gagctaacag ccgattctcc caaagtagag aaagaaaatg aaaaacaacc
    36541 aacagacggc aaaactattt caggctatgc gttgaaattc gggcaaccgt caaaagattt
    36601 aggcggcttt gtagaagtga ttacacccga agcattaaaa gaggtggatt tatcaaatgt
    36661 gttcttatta tatggacacg attattcaaa gccactagct agtgtcaaag caggtacgtt
    36721 gaaattgaat gtcgatgata caggattaca ttttgaagca gagctaaccg acacgaccta
    36781 ttcaaatgat gtgtatgaga atatctcaaa aggggttgtt gattctatgt cctttgggtt
    36841 cgtgcttgga ttagattcat tcgacaaaaa agaagatggc acaattgaac gatcaataga
    36901 taaaatcaaa gcacttaatg aaattagtgt cgtgaccgtt cccgcttatg attcatcaaa
    36961 tgtccaagta aataagcgtt cttacgaatc gtttatgagt aacaaccaag caaaacaaac
    37021 aaacaatagc ttagaatcca cttctaaagc acaaaaggag agtaataaca tggaaaaaac
    37081 tttaatcgat aacgaaaaaa cagaattgcg tgggtatgaa gaatatatcc gttcacaagg
    37141 cgaagtgcgt gatggagtca ctactgtaaa tgcagcggca gttgttcccg aagaagtaat
    37201 cggtgaagtc tttgatttga aacgttcaaa ttataactta gctcaatatg caacagtaaa
    37261 aacagtatca aatggacaag gtaaatatcc agtagcaact aaccaacaag cagtgttagc
    37321 aacaaaagct gaacttgctg aaattggtga tattgacgct gaaatgttta cttcagttga
    37381 ttataaagta gaaactcgtg ctggtaagat tgccttatca aatgaggttg tggaagattc
    37441 agcagtgaat attgtacaag aggtcaaaga tcagttagcg aaattggtag aaaacactga
    37501 caataagcat atcatggatt tattaaaaac attcactaag aaaacggctg ctacgttgga
    37561 cgatttgaaa caactataca atgtggcatt agacccagca ttaaataaaa tggtaattct
    37621 aaaccaaagc ggatattacc acctagatac attgaaagat tcagatggac gttacatttt
    37681 acaacccgat gtgacagcac ctagtggtaa atcattattc ggtatgccag tagtattgat
    37741 tgcagataca ttgtttgcca atcctaaagc tggaacattc cctatgatta tgggggatat
    37801 tgcacaatct atctttgttg ctcgtagaaa tcaagtaacg actcaatggg aaaaattcga
    37861 ttactactca caaggacttg cagtgatcgt tcgcaacgat tataagaaaa ttgatcaaaa
    37921 tgcttcagtg tatattgagt ttacgccagt tgtaacacca aaagtataga aaaatattgg
    37981 gcggcggttt atcccactgc ctttttttca ttaggagaga taaaatggta attttggata
    38041 gcataaaaaa aagtatgcgg atcgatcaca ctattgatga tgactttatt caacaattga
    38101 ttgatacagc aggcgaatat ataaaaagtg ctattgatag tagcgcaacg gataaagata
    38161 tggataatta tcagcaattt gatttggcgg tgtcattact tactcaacat tggtatttga
    38221 accgtcaaga agccagcagc gaacggatac cagtaacggt acaagcatta gtacaacaaa
    38281 tgagaggtgc ttattatgcc aatcattaag aatgtaaatg aattgactga gagagtcaag
    38341 tttaaaaaga caaaacgggt aaaagatgaa gatgggcaga tggtggatag tgaagaaact
    38401 gtatttgaat gttgggctaa tgtgcgttca caaatgctaa aagacgtgct tgctagtgta
    38461 ggtaccattc ttgaaggaac gttgacgttt attattcgat acgatcaaga ttatgaacta
    38521 acaaacgata tgaaagtagc ttggaaaaac aagagttata aaattatttc aatcaatgaa
    38581 ggaacggcgt ttaaagatta tacaacgatc atagctaaac agatttctta gaatgattac
    38641 acttgtaaac gttgtgggta tttagtataa ttaaggtagt aaatgaaggg ttagctgcct
    38701 gaattttgca agacttagct agtcgaaatc tattgttgga ctgaaaattg taggtgtggt
    38761 tgcaaacatg ctggactaag aaagatgata gatgatgaac gttcaatttt aatacaggca
    38821 tgctaggact gattggatgg ttttggcaag ctgattgtgt agaaatcaaa gaacacgtct
    38881 gttgtggacg tgtttttttt gttataattc aaaagaggtg atttaatgga caaagcagag
    38941 tttacaaatt tttattggaa gtattatcta catttagaaa atgagtttat taataccaca
    39001 gattttgtga tgctcgatga gaataatttc aaaacattct ctattgaata tcaaaaacta
    39061 ctactagcga ttggttcgga gtgtgagatt attttcaaag aactttgcgg atttgatagc
    39121 agtagcaata aaagcataac tgattttaaa gctgttatta aatctagcga attaatgtgt
    39181 ttggataatg gtgttagggt tatgaattct ttaacattga cttcactaaa accttttgga
    39241 gatgggtggc ctgaagatac accagattgg tggaaaatat ataacagagt aaaacatggt
    39301 cgttcttcaa attataagga agcaaatttg gaaaatgtgt tgtatgcttt agcaagtcta
    39361 tatttattag aggaatattt gcataagaaa gtaatactag aaggtgaagt agactttata
    39421 agtccagagt caaatctatt tacgttatct tgggaaagaa aacattcgtc gatgcaaaaa
    39481 gtgacgtttg aaaaagtgga ttcaaattat gtagctccac tcattgattt taaagaacaa
    39541 gaagaaattt aaaaaccccc acggcttatt gaagtttttg tatgccatat tgtatgccat
    39601 ttggtttaaa tcgcttgaaa aatagtgata gataactcta attcatttag tcataaacgc
    39661 tgttttgata acctttatag ttctttgata tgggtttctc attcatggcg gaagaagaag
    39721 aatagaacaa aagaatgaat taataagata taaaaaaagg gattgtgaca aacgtcttgt
    39781 cacaatccct ttttccaaat aaacgatgtt caatcagctg cccctcggca ttcgctcgaa
    39841 aaacggcgaa atatgaagaa ctattttgcc atttgtttct ctaataatca ggtcaaaacg
    39901 ttgacttcac agtctcttcc ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca
    39961 caatattcaa caaacatgag aagctgtttt ttgaaattgt tccttgtgac tcggagctga
    40021 acggcaggct cacgacctct ttcttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    40081 gttcaaccaa acgaaaatta tgaggagcta tttttgtttg gctgttctta ttacttgatg
    40141 caggacagct ttttcacaac ctcttttcgt ttagcactaa atttccttaa aaagtagaaa
    40201 agctttcgaa gcctcgtttg tttcttttac tgtataataa aagaaaagag taacaatcgc
    40261 taaaagatat aaacaagaga aaggaaatac aagatgattg gtctgattta ttttgtggtg
    40321 atcgttctgg cgaacacagt tggcgctatt tccgggatgg gtggaggcgt gttgatcaaa
    40381 ccgattcttg atcttatcgg cgcccattct gttgcaggga tttcttttta ctcaacagtt
    40441 gcggtattca cgatgtccat tgtttcgact gtgcgacaag tatcaagcgg gaaatcactg
    40501 aattggcaga ttgtcggttg ggtatctagt ggagctgttg ttggaggagt agtcggcaac
    40561 attgtattcg aagttttttt acagttattt gaaaacgaga agcatgtgca gctgatccaa
    40621 atttttttga cggtattgac cttggttttt gcgttcttct acaccaagca tcatcagcca
    40681 aaatttcatt tgacgtcatg gacttggtac cttatatgtg ggggggttct gggcttttta
    40741 gctagttttt taggtattgg tggtggacca gtcaatgttt ctttgttgat gctgatgttt
    40801 gcattgccaa taaaagaagc aacactatat tctctatcta cgattttctt ttctcaactg
    40861 gcaaaattgg taacgattgc tttaacaagt agctttatgc gctttgattt atctatgttg
    40921 ttttatgtta tccccgctgc tgttattgga ggtttgtggg gagcaagatt tagcagggtt
    40981 ttgtctccaa aaaaagtgac ctttattttt caagtaattg tcctcgtggt tcttttggtc
    41041 aattgttaca atgcctttgt catcttacaa acattaggag aatctagact aacgcaagtc
    41101 aaaatcactg aaacatattg ttctaaatga aaaaaggagt atactgtgat aaaaaaattc
    41161 atttaaaaaa ataaaaattg gctggtgatt tggatgttgc gggaaatgcg tttaggagat
    41221 gcagaagcac tacgagaaat caatgcggaa caattaggat atgatgtacc attaaccgtt
    41281 acggtgagac aaatgaagaa actgttgaag gagccagaaa aaaatttttt cttggtctat
    41341 gaagatccta cttctaaaaa agcagctggc tatgtacaag cggagtccta tgaatcgatt
    41401 ttcagcgatc cgatgttcaa tattatggca cttgctgtgt ctaaacatgc tgaaaaaaga
    41461 ggaatcggaa aggccttgat gactggcgta gaagaagcga aaagacgagg aatatcagca
    41521 ataagactta attctgcaga atacagagta gaagcacacc gattttatga acgcatcggt
    41581 tatcattccg ataaaatgca aaaaagattt ttgaaaatcc tgtaaatttt tatcaaaaaa
    41641 tacctggtca tcaagccgat acttgatgat caggtatttt ttttgactaa gaaagtaatt
    41701 gcatcaagaa acctgagatg atcaaaacga tggcaccacc aagccgattg cccatttgag
    41761 caaaagcaat aagttccatc cgattagctg cagataagac agctacattt cctgtaccac
    41821 ccatcgagtt gttgcataag ccagcagtaa tagttgattc gattggatat aaaccaaaca
    41881 aacgaccgac aaatcctgaa actactgaaa tcgcgataac acttgtcaaa cataagacaa
    41941 caaattgcca tgtcaaagca gaagccaata catttagatt caataacgca ataccgatac
    42001 cggcaagaac agctgctgtc aagtttttga caatcaagtt gttgaacatg atagctgcat
    42061 cttcgtaata ttctggtaat aggtttgcga ttttacagat aacaacgata atgatcatga
    42121 atgcgtaagc atggacttta ggaacaaaat agttacagat ttgtcctaag atgaagaaag
    42181 tcaatgatac catcaaaccg acacctaatt gtggaatgga aggttttaca tttctaggtt
    42241 ttccatcgcc aaggtcgcct ttttccatta atttaccatt accgttgaat tttggcattg
    42301 attgtcccgc acgagcaact aacgctgcac caatgatagc taggacattt gtcattgctg
    42361 tcgcaggaat caggcgggaa atgatagaag aagcgtccgt acctaaaaca tttgcataaa
    42421 tcgttgataa aggtacagct ccggctccaa caccaccagc cattgcgggc aatgagatat
    42481 ataaaacgga atttgcaaaa ccatttccga taagcatacc tacgagtcct acggcaaaaa
    42541 aggagacgac cattgaaata agtgcaacgg gaataaaacg aacagaagct ttcatcaata
    42601 gattacggtt cataccaagg atgctaccag taatcaaggc agcaatataa aagtctaaaa
    42661 agcccatatt gctgacaaag ttttctgtgg attttactac atactcaggt aagatcccaa
    42721 atgtagcaat cgcggcagag gcaaagatcg caaaaacgga gccaccacct aaataacttt
    42781 tgacgatagg gatttttgtt ccgataaaat gaaataaatt accgaaaatg accaaaacag
    42841 cgattgggcc aatcatattc gtcggtaatt ttcctaaagc cattgcaatg atcaggacaa
    42901 ggaccatgat caaataaagg ggcaaactta cgccggtaat ttttgttgac caaaaccctt
    42961 cttttttctt ttcctcaact gcagttgcgg ttgttgtttt ttgtgccatt tttacagatc
    43021 ctttcttatt ctgtatcgta tgaaaaaagc caaagagaag cggcaaaaga gcagtagttg
    43081 gctggctgct gttttgccgc agtcgctttt ttctttttag acgtcataca aagtttctgt
    43141 atgttctttt actataacaa tcttaatctt ctaaagattt atattcagga acccatttag
    43201 cagattcaac agcttctttg atatccgtga tttcttcacg attcaatttt tgtgcaacaa
    43261 ctgattttgc gacagtttca gcaatcgttt gagagaattc agtgattttt gctactggag
    43321 gcaagatagc tgctcctggt tttgttacat caacgattcc gcctaatgct cgactagctt
    43381 gagaaatgat ttcagcattt actcgtgtag ctgttgaagc aatcaaacca agccctaaac
    43441 ctgggtacat caacgcatta tttgcttgac cgatttggta agttacaccg ttatattcca
    43501 catcagcagc agggatacct gttccaacta aagcttttcc gtccgtccat tcaatcaaat
    43561 cttttgcttt tgcttctgcc aattttgttg gattagacaa aggcatgatg attggacgtg
    43621 gggtatgagc cgccatttct ttcacgattt cttcagtgaa tgctccaggt tgggtagaag
    43681 taccgatcat gatcgttgga tggattgctt ttacaactgc ttctaagttt gtcaattcat
    43741 cagcattagt gaattctgag cgtttacgag cgaatgggat ttgaccagga gtcaatcctt
    43801 ttgtatcttc aaacaataaa ccctgtttat ctacttgata gaaatgttgg cgtgcttctt
    43861 cttcaggtac accttgacgg atcatttcat ctaaaaggat gtttgcgata cctacaccag
    43921 cagtaccagc accaaatgtc aagatcgttt gatctttcaa ttgctcacct gatatattca
    43981 atccacctaa tacaccagct agtacaacga ttcctgtgcc ttggatatca tcattgaaag
    44041 tagtgatttt atcttcgtat ttttctaaga tcgttgcagc atttcctcgt ccaaaatctt
    44101 cccaatgtaa taacaattct gggaataatt cagtagctga ttgaacgaat tgatcaatga
    44161 attcgtagta agtttcgcct tctacacgag catgacgatt ccctaagtat aatggattat
    44221 tcaataattc ttcgttatta gtaccagcgt cgattgatac aggcaagact tgtgcaggat
    44281 tgataccagc cgcagcagtg tagaccatca atttaccgat agcgatgtca acaccgttca
    44341 caccccagtc acccatacct aagattcctt ctgcatctgt cacaacgatc aaacgaatgt
    44401 cgcgtccatc tgctgcgttt ttcaaacttg ctttgatatt ttctggttcg tcgattgaaa
    44461 gaaatgcagc atcttgtggt ttcaagaaaa tttcgttgta ttgttcgatt gaatccgcaa
    44521 cgacaggatc ataaacgaca ggcatgaatt caactaaatg ttcgcccatc aatttataaa
    44581 acaatgtccg gttcgtattg aatagattca ttaagaagat acgtttttcc aaatcggtct
    44641 gtttgcttaa gtattgaccg taagcttgga cagattgttg ttccaatgtt tggacagtac
    44701 ttggaagcat accagtgatt ccgtattttg cccgttcttc ttttgtaaaa gctgttcctt
    44761 tatttagaaa aggattattt aataagttta gccctgtaag catgaatgat tcctcctaag
    44821 ttttaataca aacgaagtat aggagtcaga tgaggatata gtcaaaaaga gcaggtataa
    44881 taaattttat ttatatgagt aatagagtca aaagaaatca aaatagcggt atttgttctt
    44941 tttatatcaa caatccttgc ttgattacta tgcgtatctc ttatatgcta aagaaagaaa
    45001 agtagaagaa taaaggatat gttagataga taaatagaat ctctgaagaa taaatcacgt
    45061 gatcgtgacg ataaaataca cttacgtcag gtgataaaaa atacataagt agaaaactaa
    45121 gaggaggaac agaaaatgaa agaagttcaa ttgatcagaa aaagcgaact atcagaaggt
    45181 ggttgcaatg catgcggcgt agtagaagca acaagctata cattaaagct tggtgccaac
    45241 aaagcgataa tctctgagtt gacagtaggc ggtcttgtgg acagtctggc gcttgctgaa
    45301 ggttttattg gagaagatat ttatgagatg ttttcagaaa tccgtcagtt aaaaaaaggg
    45361 gaaaactgca tagaggttca tcatgagagc ccgaatgtga gattcaaacg tggtgataat
    45421 gagatgatct tcaataatca tgtgtccgat cacaccgagc tatatggaat cgtgaatcaa
    45481 atactaactg agctttttgg attgggacct tatgcgttca aggaagaaaa cggtaatcca
    45541 aaattaaacg aagaatggca agaaacgatt gaaatacaaa gaaacaaccc gcacttattt
    45601 caataggcat ccacagtatt tcgtaaaagg gactttgaca cgacttttgt caaagtccca
    45661 tcgtttttta tctatttgtg ttctaaacat ttctttcaga gtagtaacaa ctggaatgac
    45721 tgaaatgtaa acagattatt tgcctgtatc aatacggaag agtaatataa agtgtgtaaa
    45781 cgctatcata ttattcattc aattttttat agagggaagg cattgaaatg gaaaaaacgt
    45841 atcaagcagg tacaaatgaa ggaatagtgg attttatcaa tatggaagat ttagaaatag
    45901 ccgcttcaca agtgattcca gctggtggtt atggttacat cagcagtggt gctggcgact
    45961 tatttactta tcaagaaaat gaacgggcat tcaatcatca gctgattatt cctcatgtat
    46021 taaaagatgt agaattaccc gatacaacta cacattttga caaagaaacg ctgactgcac
    46081 caatcatcat ggcaccagtt gctgctcatg gcttagctca tgtgaaggcg gagaaagctt
    46141 ctgcaaaagg tgtagcagat tttgggacga tctatacagc aagttcttac gcttcttgta
    46201 cattagaaga gataagagaa gcaggcgggg agaaagcacc gcaatggttt caattttata
    46261 tgagcaaaga tgatgggatc aatttggata ttcttgaagt ggccaagcgt aatggagcaa
    46321 aagcaatcgt attgacagcg gatgcaacag ttggcggaaa ccgtgaaaca gaccgcagaa
    46381 atgggtttac cttcccactg ccaatgccga tcgttcaggc ttatcaatca ggagtaggcc
    46441 aaacgatgga tgctgtgtac aaatcttcca aacagaagct tagtccaaaa gatgtagaat
    46501 ttattgctgc tcattctgat ttgccagtct atgtgaaagg tgtccagtca gaagaagatg
    46561 tctatcgttc attagaatct ggtgctggcg gaatctgggt atctaatcat ggtggccgtc
    46621 aactagatgg tggtccagca gcgtttgatt cgttgcaata tgtggcagaa gcagtagata
    46681 aacgtgtacc aatcgtgttt gacagcggtg tacgacgcgg acagcatgtt ttcaaagcaa
    46741 tcgcatcagg tgcagatctt gttgctattg gcagacccgt tatttatgga ttgtctttag
    46801 gaggaagtac aggagtccat caagtgtttg atttctttaa aacagaattg gaaatggtca
    46861 tgcaattagc tggaacacaa acagtagaag atatcaaaaa aataaaattg cgtgagaatc
    46921 gttttattta agaaacaaat gacgaaaaaa ataggagtgc ggcagatgat gccgtactcc
    46981 tatttttata gttcaaggat gtcactaata gaataatttg aaacgaaaaa ataaaattga
    47041 aatatttttt catacttaat aaaaaaggaa gaatattcat gtaaacgatt ttattgtttt
    47101 tttctctttt atacaaaaag ggtcataatt tcaagtaagc ggatgaattc atgaggattt
    47161 tttgcatact gtctctacta tacttaggtc actaagaaaa aggagtgatt tattatgaaa
    47221 aagaacatga ttgggacatt attactttca acacttcttc taggcggctt ggcaacacca
    47281 gcttttgctg agggtcaagc gacttcaaaa ggagatatta cctttactga accaacaaat
    47341 accgtggagc ctcttaaccc tactgatcct tcaaaaccag tagagccagc agatccggaa
    47401 aatccagcaa ctggccagac aggtccttta acactggatg tagttcccga attgccattt
    47461 ggtacacacg agattgaaag cggaacaaaa acgtatcaag tagacgcttc aaaaaacgat
    47521 acaccttatc tccaagtatc agatcgtcga ggtgtaggtg cagatggcca agcacaaggg
    47581 tggaacgtga cagtttcggt ttcagatttt gtaaacggtt cacaagtttt gcaaggcgct
    47641 gaattagatt ttggaacatc cactgttaaa agtacgtcag ataacgaatc cactggacca
    47701 actagtcaaa ctgtaactgg gttgagtaaa gcaagtgcag cgacaccaat atttacagca
    47761 gcaaaagatc aaggtctggg aacatggctt tctgtttatg atccagcaaa tatcacactg
    47821 aaagtgccga aagcagcagc tggaacattc acggctgatt tgacttggaa cctcgtggct
    47881 ggcccagtag catagagtat gaaataaatt caacaaactg cggtgaaaat actataggtg
    47941 aaggattttt taccgcagtt tttgttgaaa ggatgtggtg gacaatgaat caaagtaaac
    48001 gactgtttgt ttcgtttttt tctattttaa gtctattttt catttttccg tcaatcagcc
    48061 aagcaaagga ttcagcagga gactttggaa tcaaaccggt atttccagag aaccaaatag
    48121 ataaagcaat cggttatttt gatttattag tggcaccgga acaaaatcag acactagaag
    48181 taattatcag caattcttca gatgaagaac gaacatttga ggtctctgtc aatccggcgg
    48241 taacgagtga tggtggaacg atcgattata gtcaaaaaaa cccaacgtta gatgagacat
    48301 tgccgtttga tgtccgtgac gttttgctca ttgcgaaaaa ggaaatcaat gtatcagcac
    48361 atgcagaaac aactgtacct atagaagtga aaatcccagc aaaaagcttt aagggacgtg
    48421 tattagcggg tattcatgtt tcacctaaag aagaagctga aacagaaaat gctacagaag
    48481 gagcacaaat caaaaatcgt attgcttata atctcgcagt cgttttacaa gaaagtcaag
    48541 agacgataga accggatctg aaacttttat caggagattt ggacgaggtc aacgcaaagc
    48601 caactgttca gcttcgtttc caaaatcctc agcctaggat tatcagcaat ttgatattta
    48661 cgagcaagat cttttatgaa aatcagcttt atattgaaaa tacgtctaat gcatttttag
    48721 ttgcaccaaa ttcgaatttt cacttgaatc ttgatttagc tggtgataaa gcaaaagccg
    48781 gcgattaccg cgcagaaatc atagcaaaat caggcgacag taatgaatgg cgatttacac
    48841 aaaactttac gatcaaaaaa gaaaaagcac aaaaagtaaa tgaaaattcc gtttttgcag
    48901 tacaagaaca atctttccca tggctttatg caggattggt tgctgcagtt ctagtgttat
    48961 ggttgatttt gctgtttttg tgggtccaca agaaaaagaa aaaaggagaa acaacatgat
    49021 gaggtgattt cgttgaaaac atcgatctta ttgggaggga ttattgcaag tttgctgttc
    49081 ttcagctggc aagagccagt ccaagcagat accaaaagtt caggtacgat tacattttat
    49141 gatctaagtc caattccgca tccaagtggt tcagtcaatg gcgcatcaaa acagcagaca
    49201 cagctaccga tttataaaga atctaatcaa catggcgtat tcccggagac gaatgaacaa
    49261 aagatccgtt ttctttattt gatcattggt atagaattat ggcttcttgc gtggttgttg
    49321 ttatggagaa gaaaggaaaa ggaggatgaa catgttgcta caaaataaat tttcattttt
    49381 tcgctccttc ttttttactt cgctttttgt tttaagtata tttatctttt caccttcggc
    49441 agaagctgtt ggagcaacgc ctcctgccat ttcttatgat ccatacacgt atgaaaatat
    49501 cgtacctcca aatggtggac gtagtgcagt caatggcggg aagcttgtcc aactaactgg
    49561 ggctaaccaa ttgcctaaat tgactgctac ttatttgaac gatggttcaa atgatccttg
    49621 gaaaggtggc acggtccgtt ttcattggga aagccatgtg aaactaacaa atgtacttga
    49681 tttggtgaat atcactgcac aaggatttgc atttgtggcg atcatcaatt tgccggaaca
    49741 tgtatcaagc agtgatgtat taaaagcaat tgactggaac aacgcctact tgtatattgc
    49801 cggtgccccc atcaaggtta ctagcggaag tttattatca gcaggaagcc ataccttgcg
    49861 ttttgggatg gggtcgtggg atacaaatac tctattgaca gatattattg ctattattac
    49921 gcgaggcgaa ttcaacgtgg acgatatccc gcttcaattt gatgttacag ctaatgtcgc
    49981 gaagatgaca gaaaacggag ataaatatga tacgagtcca aacaaaattt tgaccaaggg
    50041 taaattacaa ccttctatgt ctaaaaaggc cgttttttct gttgattttt atgattcgaa
    50101 taatattatc caagatcgta tgttcaattc gattttagct ccagctgcag gtagatttta
    50161 tcctaaatta actggcggaa acttgaatcc aggagcatat gcattttctg caagtgcaga
    50221 gataaatacg tggaatagtt acatctcacc aagtgattca acaggaaaat ataatacatt
    50281 tgctgatacc acagaatacg tggatggaaa taaccagaac cttccaggct ctaccaatag
    50341 tcggacattg acgatggggc tgactgaagg acgttttgat cagttgccat ttagtcgctt
    50401 tgatcgcgta gtcaactatt ttacaggaaa aaacgttaca caaggagcaa tactgactca
    50461 tactccagca agcacaagtg gattgaatca gacagccaat atcgtgtaca gtgggaaaga
    50521 tgcgcaaggc ggattgcttt ctcctgttgc tttaaaggta ttggagcagt acaagctaga
    50581 cggaacggtt ttaccaacaa acttaacagc cgatagtcag tggggaaagc tcctttcttc
    50641 tgcatcgtac caagtcacgg ccaattggtc agcaactgaa ctaaaaaagg gttcgatcca
    50701 ttacaggctt tattcgaaag gaacccaaaa attagcgaca ggatggacag atcaactttt
    50761 ccaaacgatc caatcaaata acgggggtgt aaatacagct acttctacac tacctgcatt
    50821 ggcttcaggg caatattatt ttgattatcg cttggtggac gatgtactta ctaacatgta
    50881 tccgtctttt gcgtacaaat ggcagtctga acaagctgga atcaccactc ttcctcaaat
    50941 aacagtggct aattttccat ctgtgagtgc agatagtcag ctaaaaaacc ttagcagaca
    51001 acctgagaca ggagatcccc tgtctgctct atcaggagat acgattcaag aaaatgtgac
    51061 attcacgtta acaaaatctg gggatgcgat aaccgacaaa aaaatcacga tctcacttcc
    51121 tgaaaatact acttatatca atggttcctt aaaattaaat ggtacaacta tcgcagatac
    51181 cggcattcaa caaggagttt ctgtcccagc agatctattg agcaaaatag gagataccat
    51241 tcatcttact tacaactatc aattaaatac tgtagatact tctttacaat ccgtcagcat
    51301 actgacaaag gcagctgtac tttccagcaa tattacatta gcagatggtg ctaagcttcc
    51361 taacccagtt gtccaaacat ctgctaaaac aattcttgtc cccaaacaag aattaacatt
    51421 agttaatgta ccagatgatt ttacatttgg taacgatctc cccaaaccgt tgaagacaag
    51481 ttattatgaa tcaaaaggcg atttttcttt tgatgtgcgg gatactcggc ttccaagcac
    51541 gtctccttgg caactgacag gtacattgac ttctttattt aagaatgatc aaggtcaaga
    51601 attaagcgga acaaaattat acttcaatca ttctggatca aagcaattga ttcagcaagg
    51661 tcaaaacacg ttgatttatg agagtgatgg aacagctaaa ggagaggttc tcgttgactt
    51721 tcccgatacc gatggtttac tcttagaggt aaatagcagt acgaatgctc aacctggcgc
    51781 aacgtatcaa ggcatggtca catggaaact gacagctggg ccaaccagct aagtgattag
    51841 aatttgtatg gatctgtgat aactgtcaca gatcctcttt ttcaaggatg agaaggaaac
    51901 ttctgctgtt aaaatcagaa atgcttgaag gagtggactt ggaacttttc agtttgcttg
    51961 aaaaaaatga acaacggcaa atcaaacttt tccaactttt attagaggaa aaaggagagg
    52021 tttccgttaa taacgtactt acccgcttat cagttgatcg ggctaccttg aaagaagatc
    52081 tgttttattt acaagctaac ttggaaaata tcaaacaatt ggaaattgtt atccaagaag
    52141 ggaatatttt attaatcaga catggaaata tagcagcagt cgatgtttat tatcattgtt
    52201 acttgcgcta ttctacaaaa tatcagatac ttctttatct gttggaaaat ggctcatttg
    52261 atcggcagaa acttgtcatg gaacttaata tcagttcggc tactttgtct cgcagagtga
    52321 aggaattaaa tgattcttta aatgagtttc atctgaaaat aaaaagcggg cggctgaccg
    52381 gtccagaaag tcaaattcgt tatttttatt tccaattatt ttggtttgga cgtccctatc
    52441 ttgaaaacaa aaaagaatac agtgatttat caggaaacac ttttttagat ctgctgcaaa
    52501 aagagtttag ttttgctatt acagaagaag gacgattgaa atttgaaata tggctttcca
    52561 ttatgaagaa gcgaatgcgc tgtacgaaaa aatccaaacc agattatagc aatcttcctg
    52621 ttggattgtt tcaaacgaca caatttttat tggacctcca tacatttttg acccgttact
    52681 tctttcaata tgcctttcct tggaatcaaa atgaaacgtt ggttttttat cttttcatga
    52741 tcagtaactt tacattagat acacataact cgcatgtcca ggagtttttg ataagcgaaa
    52801 aatacaaaga cgacgtagta gctcaattga atcgaacgtt taaacaatcg atagaagaat
    52861 atttctcttt ttatgttttt ccagaagaga tttcgcaaaa gattgcgttg acagtgatgc
    52921 aaaatcacgc tcattttgcc tattttagag gatggatgaa tatttttggc caagaaggat
    52981 taaaacagcg gttaactgaa gtagaagata aacaattcct ggttttatgt gaaagattgg
    53041 tcagacaaac atttgctatt gttgatttta ctgaagtaga cgaggaaaat gcccgctttg
    53101 aattatttgc tcgctatgca tctattttac ggacgctgat caagcagtta gaattacgtc
    53161 tgcaaattgc ttatgatctt ccttatgaaa ggctaatggc ggatatcgtg aaagaaatca
    53221 tccaggaaca attgctgaat acgatcaaat atgatttagt cgaatacgag aaagaaaagc
    53281 agtatgatat cattttgact agccaattga aagagtaccc gtcacagaag tccgctaaag
    53341 tttttgtatt cactagcaac gaatataatt atgatttccc ctatttgaac caatttttaa
    53401 aagaatgtta tttagaaaaa ttgaatttaa ggatgaataa aacatgaaaa tcgtgatcat
    53461 tggtgcttcc catgcaggca tcacagctgc cctaaatcta agaaaacttc aaccgaaagc
    53521 agaagtttta ctgatcgatg aagatcataa agatggacta ggctatgttt caaatggaat
    53581 caacctttat ttaaaaggaa agatacgtag tttagctgaa gttgcccata acatgcggac
    53641 tcttcaagaa agtggggcaa aactgatcac cgaatggaga gtaactgaac ttgatccaga
    53701 caaacatcag ctcatcctcg catccaaaga aggaaaaaac gagaccataa cctatgataa
    53761 attaatcgta gcgacaggtt cctctcctgt caccttgtat aaacaaattg aagctgaaaa
    53821 tgtctacacc tataaaaatc tagtgcaatc taagcaagta cttgccgctt taaaagaagc
    53881 aaaagaagta gtgatcttcg gtgctggtta cattggattg gaattagcag atgctttgag
    53941 aaataaagga tacattattc atctagttga ttatatgccg aatgttttat caagatattt
    54001 cgataaggat atgatcaaca gttttcaaaa tcagctgcaa actaagcaaa taaattttta
    54061 tccaaatgaa ttcttgatcg attggaaaaa atcagaagag aaagtagtat ccgtacaact
    54121 tctgtctcaa acaataaagg cagatatggt cattttttct gcccaaactc gtcctaatac
    54181 gacattgtta aaagaaaaag tggctttgta tgaggatgag actgtgatag tcaatgaata
    54241 tctgcagaca agtgatccgg acatctatgc gattggagat atcgtcccag tcagttttga
    54301 caaaaataaa cggcatctct ttttaccttt agttactcga gctgtacata tggcaagagc
    54361 agtagcgcta actttgagtg gtcagccaac cgcttacgac ctcagacaaa agataacagc
    54421 agcagtcatt atagactact ttttaggaac tgtagggctg acagaagatg aagcgccatt
    54481 cttggaacaa aataccggtt cgtgttcagg agaatttgat ctatttccac aatacgacga
    54541 agaaaacaaa acagtaaatg caaagctgat ctaccatccg gatacattag aaataattgg
    54601 cggacagctg ataagccaag aattcttatt atcagatttg aacctgcttg cggatatcgt
    54661 gaaacacaaa actacgattc cacagctagc agttgaggat tttggatttc tgtctgaata
    54721 cacctctcgt tttcactacc tcaatgagtt agcttttaaa gtactagcta agagagcaaa
    54781 tagcaataga gtagataaaa gactgtgaga aatatcacgg tcttttattg tgtggggata
    54841 taataaatat taatcaatcg agaataaaaa attcatataa tgaaatagcg gaagaatttt
    54901 ttgagcataa tattcgccaa aatttatcaa gtgataaaat tgttgtataa aatgattatt
    54961 tcaggaggag gagttaaatg tttttagcat taaatgaaat tatgcattca aagttacgtt
    55021 atgcgttagt tgcaggggtg atgtttttga tcgcctacct ggtatttttt ttgactgggt
    55081 tagcttatgg cttagctcaa gataatcgaa cagcagtcga caagtgggag gcagattcga
    55141 ttgtgctttc gaaagatgca aattcaaatc tagggatgtc catgattact aagaagatag
    55201 cagaagaagt cgaaggtggg aaagtggcgt atcttgccca aacacctgga gtcgtgacta
    55261 gtaaagacag tacagaagaa ggaaaaatca atgttagctt cttcggtatc gataaaaatc
    55321 aattcatcat gcccaatctt gttgaaggaa aagcttttga taacgatgat gaggcagtag
    55381 gcgatattag tttgaaagaa gaatatggtc tagccatagg agatacagtt aagttatctg
    55441 gtagtgacaa gacgtttaaa ctaactggct ttacggatca tgccaaattc aatgtctcac
    55501 cagttctcta tacaaccatc aatgcttacc aagaaatccg cttcgaaaaa gaagatacaa
    55561 gtgaaaatgc acggattaac gcaatagttg tcagaggtaa aatcagtaat ttacctgaag
    55621 atttagaaca gatcaaaatt tctaaattca tcaatgaatt acctggatat aatgcgcaag
    55681 tattgacttt tggatttatg atcggatttt tgatcgttat tgcggctatt gtgatcggga
    55741 ttttcattta tgtactgacg atgcaaaaga ttaatatctt tggtgtaatg aaagcacaag
    55801 ggatcacagg aggatttatt gcgcgttctg tagtggcaca aacattcatt ttatcatttg
    55861 ttggtattct tcttggtcta ttaggaacag tgggaacatc ccttgttcta cctgatgcgg
    55921 ttccattcca aagcaattgg ctgttttttg gcgtcattag cttgttgatg ttagtagtag
    55981 ctgtgttagg cgcactattt tctgttcgga caatcgtaaa aatcgatccg ttgaaggcaa
    56041 ttggataaga aggaggaaga acatgaaagc aatagaattt aaagaagtag aaaaaagttt
    56101 ccaagatggc gaccaagtag ttaaagcatt gaaagaaaca accatgagta tcgataaagg
    56161 ggaatttgtt gcaatcatcg gtccttctgg ttcaggtaaa agtacttttt tgactttggc
    56221 aggcggattg cagacgccta gcagtggctc tgtatttatc aatgatcaag aattcagtaa
    56281 tcaaaaagaa agcgaacgcg ttaaactacg ttttaaagaa attggcttta ttttgcaagc
    56341 ttccaatctg gttccttttc tgacagtaaa aaagcagtta ttattagtag ataagattaa
    56401 acatgaacat cgtcaaaaag aagcaaacga attatttgag cagcttggcg tggataagtt
    56461 gatcaacaag tatccagaag aactatctgg tggagaacgg caaagggtcg caatcgctcg
    56521 tgcattgtac aatgatccgt ccattattct agcagatgaa ccaactgcta gtttggattc
    56581 agaaaaggca cgagaagtag tcgatatctt ggcaaaagaa tcaaaagaca aaaataaagc
    56641 aacgatcatg gttactcacg atacacggct gattgacaaa tgcgatagaa tatttatgat
    56701 ggaagatggt gtattgaaag aaaaagaata accaaaaagc aggtggaaaa atggtccatc
    56761 gatacaacaa agatacacag caaaaaattt tgaaagaagc gactgacttg tttatgtcca
    56821 agggatatct cggcacttca acaagagaaa tcgcacaaaa agttgggatc acccagccca
    56881 atctctatca ttatttcggt gataaagaaa aactatatac ggcagtttta gaaaaccact
    56941 taaaagatgt tggaaaagct ttgagagaaa tcgttcaaac aagcgaaacg ggatttcaag
    57001 taaccttgac aaaaatggcg cagtttttga ttgagacaca tctggtcgat ttgtttctga
    57061 tgcttcacga tcttgaaagc aatctttcaa aggaaacaag agatcatctt ttctttctat
    57121 ggaagaaaaa ttatcgtgaa ccttttgaag aaatcttttc agcaaaccaa tctgtgctaa
    57181 gaaatgggat ttcaaaagaa atcgcagcaa ggcatttttt cttagtgtta gcaccttaca
    57241 tcactcggtc gaaaagacct gtggaagaac cgctaaatgt ggcgcaattg atcgatttat
    57301 atttgaatgg tgtattagga gaagcgtaaa gctgttatat agggagaaaa tagttaggtg
    57361 aagaagcaag atatgacgaa gatcgttata tcttgcttct ttatataggt agtttttcat
    57421 aaagacaaat ttgttattct ctcatgatta tcagggtata tttttattta ttatgtcggt
    57481 gttccaggaa gtgttttaat ttaataacga agaaattaat tgtttgtaga atgaaggaaa
    57541 ctatttattg ggtagatgaa tcaccacgaa tagtcaaaag gagatttcag gatatgccaa
    57601 aaatgaaaaa aatcatttta gcagtaatgt tattttctac ggttatatct tttacagccc
    57661 cttttttgct tacttaagct gattttaagt tttggccctt agtctggatc tacgaggtga
    57721 gagtcaagaa tgaaaaaaat aattggagct tttgtatttg caatcattat cattattatc
    57781 tgttttcaaa tagatgtttt tggtcaaaaa aagatagttg gacaaacggt cagcagtacg
    57841 aacagtacgg ttacaaagac aaaggaatca tttgatgata cagaacaaga gacattaatc
    57901 aaaatcggaa aaaatgaccg tacactatat acagatgact cgctaactac cccacttgca
    57961 actataaacg gcggagagct ggcggagttt ctttctgaaa caaaaaatgc ttatcagatc
    58021 aagacaaatg atggctatac tggatattta gccctcgaag atggaaccaa agtcacaaag
    58081 aatatacaaa caaaaccgga agagctatct gatgcagtga ttgtattaga tcctgggcat
    58141 ggcggaaatg atactggggc attaagcaat gatgaacaaa cagaagaaaa agatataaca
    58201 ttaagtacag ccataaaagt aaaaaaggcg ctagaggatg ctggagcaac ggtttatttg
    58261 acccatacta cagatgaatt agtacagctt ggagatatat gcgactacag tgaagaaaaa
    58321 aaggcagatg tctttatcag tttacacgca gactctacgg aatatgcgaa tgaagcaact
    58381 ggaatcacga catattatta ctatggacaa gaggaaacat tagcacagac aattgctgac
    58441 tccttcactg atctgcctct ggattcaaga ggaatctcca ctgggaacta tcaagttctt
    58501 cgagaaaatc ttcaaccttc tattttaatc gagatgggtt atatgaataa tgatagtgat
    58561 ttaaaggaat tagtaacaga cagttatcag cagcagattg ctgcaagttt gacacaagca
    58621 ttgaccactt attttcagta ataaataagc ccagccaaac aaaaatagct cctcatattt
    58681 ttcgtttggc cgggcttatt atcgcagatg caagcttttg aacaccgttt atttttttag
    58741 tagcttcaaa gctttttctg tatttgcaaa gtgaagtttg gcatattttt caagcatagg
    58801 catgccgcct ttctccaata gttttgccgc tacttggtca gatttcgttc cagctcctga
    58861 tggaagggtg attccagctt cagctgatgc tttgtgtaac tcttccaaaa agctggcacg
    58921 actgatgata gaagcagcag caacagctaa atgatattgt tctcctttag taacgaagta
    58981 cagtttttct tgtaattgat ttttttctaa acgcgcatat tttttaaaat ttgcttccgg
    59041 cgtaaactga tcgatcaaaa tcgcttctgg tttttctggc tcgatttttt ttagcagcaa
    59101 atggatcgct tgattgtgca aagcgacttt catccgaacg gcattgtatt ttggttggat
    59161 ctcattgtat ttttctggag ataagacaag cagtttatag gggatggttt tttttaagat
    59221 gtcagcaagc tggatgattt ggctatcttt taactctttt gaatctctaa caccaagttt
    59281 acgtaattct gcaagctgat cacgtttcac ataagcagca catactgtta gcggaccgaa
    59341 atagctgcca tttccgacct catcactacc taaaacggat aattgactga tattttttgg
    59401 caaagaagag gctgtttttg ctggcttggc ttgtttatcc tgtgcctccc cccatttatc
    59461 agcttctatt gctgcattcg taccttgaaa catcactttt ccggatgtat aagcggtaat
    59521 ggtcgtcccg tctttttttg cagcaaagct agtataagga atctgtttat ttaacaaata
    59581 agagcgatag tatgctttca tttgactaag ttggtcgttt gttgctttga taatgacgtt
    59641 gctcataaaa ttctcctcgt ttttatacat aaaactatac cgcaactcac gacttttcgt
    59701 tttctaagag attgcgagac atagcatttt tttaaagttt atcatataat agttaagtat
    59761 gtgaaaaata tttgtggaga acggtgaaag aaccaacttc atggtacact aaataaaact
    59821 ttcatcaaat aggaggaaat gattcgtatg gcgcacgaaa aaacacgcta caaagcggtg
    59881 attgccaacc aaacatatac tattatcgga cgggaaacca agcatcatat ggatatcgta
    59941 acgaagctga tcaatgaaca gttagcagaa ttgaaacaat tatcaccaca aatggataat
    60001 gaacaagcag caatcttgat ggctgtcaat gcgctttcgg atcaattgaa gaaacaagaa
    60061 agaatcttgg agctggaaga agaaacagca gaattaaaga aaaaaatgat caaatttaca
    60121 gagcttgaaa atcgtgtgaa acgcattgaa gcaatcgaga acgaagctcg ggaagtattg
    60181 aaagaaaatg gtcaagcaga tcataccatt aaaaatcatg tggaagcaca acagatttta
    60241 aacgagaaac gaaaaggaca aattaaacaa aaatcttcta attaagatcc ttttatcaaa
    60301 ataagaaaaa tttaaaaaaa gttagaagag aaaggaacgt agcatgctga gtttgctgat
    60361 tttattttta ttgctaattg cgtttttttc tggagcacgt cgcgggtttg ctttgcaaat
    60421 catattcgcg attggatatg tattgtcatt tattgctgcc cagcattttt acaaaccatt
    60481 agcaagtcat ttggaattat acattccata cccagctgtc acgccaacat caaagttagc
    60541 attttttgat caggtatttg cctttcacct agatgaagct ttttatgcag gaactgcttt
    60601 tctgattatt ttgctaattg gctggctgct tacccgattt gtgggggttt ttgtccatgg
    60661 attgacgtat gtgccgatct tgagacaggt agactggatt gcaggaggca tcctgtcact
    60721 aatcatggct tacgttacta tatttttgat tttgcagtta cttgcctttg ttccattaga
    60781 tgtggtccaa aatcaattcg gcgagcatag tttcgctcgc tttatcgttg agagaacgcc
    60841 atttcttacc aacaagatgt atgagctttg ggtaacgaat attttgaatt aaggaaaata
    60901 gtgctggaaa atatttcgtt ttttgtacag agtagattat ttaagaaggc tgcgtagcgt
    60961 atgtccggca cagctctttt ttcatagtga aagataaggt gataaaatga ataaccgaat
    61021 ccttgaaaca ttggaatttg aaaaagtcaa acagatggtc agacaatttg tagtgactgc
    61081 acaaggaaaa gaagaactgg cagaattggt accagtgagc gaggaacaaa cgatcacaaa
    61141 ctggcttcaa gaaactgagg atggattgaa agtgcaacgt ctgcgaggcg ggatccctat
    61201 acctaaactt gaaaatatcc gtccccatat gaaacggatt gagatcggag cagacttgaa
    61261 tggagtggag cttgcacaag tttccagagt attgtctacg acaagcgaat tgaatcgttt
    61321 cattgatgat ctttatgaca gtgaaatcga atttgcacgt ttgtatatgt gggcagatca
    61381 gttagtcacg atccctgttt tgagccgccg tttaaaggaa gcaatcgatg aagacggacg
    61441 cgtgactgat gacgcttctc ctgagttaaa gagcataaga cagaacattc ggcgaagtga
    61501 acaagctgtt cgtgaacagt tagatgggat cgtccgtggg aaaaatgcaa agtatttaag
    61561 tgatgcgatc atcaccatgc gaaatgaccg ctacgttatc ccagtcaaac aagaataccg
    61621 cggtgtattt ggcggtgtag tccacgatca aagtgcttca ggacagactt tatttattga
    61681 accaaaacaa gtggtggatt tgaataatcg tttgcgtcaa taccaaatcg ctgaacgaaa
    61741 tgagattcaa cgtattttaa gtgaactttc tgctgaactt gttccccatc gtcaagaaat
    61801 catccacaat gcttatgtga ttggtaaaat ggatctgatg aatgcaaaag cacgatttgg
    61861 taaagaagta aaagctatcg tgccgggaat cagtgaagac aatcatgtcg tcttgaaaca
    61921 agcaagacat ccactgatcg atcaggaaaa agtagtttct aatgacatca caatcggaaa
    61981 agactatcaa gccatcgtca ttacaggacc taacacaggt gggaaaacga tcacgttgaa
    62041 gactttaggt ctgttacaac tgatggggca agcaggatta ccgattcctg caggtgaaga
    62101 aagtcaaatc ggtatttttg aggaagtatt tgcggatata ggagacgaac agtcgattga
    62161 gcaaagtcta tcgacctttt cttctcatat gacgaacact gtagatattt tatcaaaagt
    62221 caatgaaaaa agtctggtat tatttgacga gctcggtgca ggaacagatc cgcaagaagg
    62281 tgctgctcta gcaatcgcaa tcttagatga tcttggtaaa aagtctgctt atgtgatggc
    62341 aacaacgcat tatcctgaat tgaaagttta tggatataat cgggcaaata cgattaatgc
    62401 cagcatggag tttgatgtgg atacattaag tccaacgtat cgtttgctga ttggcgttcc
    62461 tggacgaagc aatgcgtttg aaatctctag ccgattaggt cttgatacag aagttattga
    62521 tgaagcgaag cagttgatga atgatgagag ccaagattta aacgaaatga tcacagattt
    62581 agagaatcgc cggaaaatgg cagagaccga atatctggaa atgcgtcatt ttgtttctga
    62641 ggcacaagaa cttcacgatg atttgaaaga agcttacagc tatttctttg aagaacgtga
    62701 aaaagaaatg gaaaaagcaa agaaaaaagc aaatgaagtc gtgtcagagg cggaagaaaa
    62761 agcagaaaaa atcattgctg atattcgtaa aatgcaacag cagattgggc aaggaaatgt
    62821 gaaagagcat cagttgatcg atgcaaagac ccaattagca aatctacatc aagaagaaac
    62881 tttgaagaaa aataaagtat tgaagaaggc gaaagaacaa aaaacgttga aaccgggaga
    62941 cgaagtgttg gttacgactt acggccaaag agggacgctt ttacgtaaaa acgggaatca
    63001 gtggcaagta gaaatcggta tattaaaaat gaatgtgtcc gaagacgagc tgacgcctgt
    63061 tgctcctcaa aaagagccga cacaacgagt tatccatgct gtacgatctg aatctagtag
    63121 tcacgtgccg aatcaattgg accttcgagg aaaacgatac gaagaagcgc taagtgaggt
    63181 agatcaatat ttggattctg ccattctagc tggttatcct caagtgacta ttgtccacgg
    63241 aaaaggaaca ggcgcacttc gcaagggaat tactgattat cttaaaaacc atcggagcgt
    63301 caaaagcttt gagtttgcac cggcaaacca aggcggaaat ggtgcgacaa ttgttaaatt
    63361 taagtaaagc ttactaaagg taagcagatg gttcgctatc caagtataga aatgctatac
    63421 tagtcataga ttttaaaaat ggaggtcttt gatatgtcac aagtaattac agataagaca
    63481 tttaatgaag aaacagataa aggacttgta ttgattgatt tttgggcaac ttggtgtggt
    63541 ccatgtcgta tgcaagcacc gattttggat cagttggaac aagaatatga tgaagaagaa
    63601 ttcagaattg ctaaaatgga tgtggatgaa aatccagaaa ctcctcaaca attcggtatc
    63661 atgagcattc caacattaat gttgaaaaaa gatggtcaag tagtagaaaa agctgttggt
    63721 gttcattcaa aagaacaact acgtcaaatg atcgatcaat atttataaaa acttacagca
    63781 aaaataagct gagggtggga ctgaatccag ttccgtcctc ggtttttttg cggttgagtt
    63841 aaggttattc aaattaatat tttttgagta taattacgtt gtcaggctgg attgtttttt
    63901 tgacagacat gctataataa ctttaattga atatgaaggg gagtaccaga tgaacgaacg
    63961 aatcaaaaac aaattagctt tacttcctga tcagcccggc tgttacttga tgaaagataa
    64021 aaatggaacg atcatttatg tcggcaaagc caaaatatta aaaaatcgtg tccgctcata
    64081 ttttactggt agccataata cgaaaacgga acggcttgtt agtgaaatcg aagactttga
    64141 atacatcgtt acagaatcga atatcgaagc attgctctta gagatcaatc tgataaagaa
    64201 aaatgatcct aaatacaaca ttatgttaaa ggatgataaa acctatcctt tccttaagat
    64261 aacgaatgaa aaatatcccc gtttggtgat tactcgtaag gtactaaaag acaaagcact
    64321 gtattttgga ccatatcccg atgtcggtgc ggcgaatgag acaaagaaga tcctcgatcg
    64381 attatttcct ctgagaaaat gcaaacctag tcagacgaag gagccttgtc tttactatca
    64441 tttgggacaa tgtctgtgtc cttattattt cgacgtagat ccgaaagtct atacgaaaat
    64501 cgttgatgaa gtgaaaaact ttttaaacgg aggacacgaa aaaatcgagt cagagatcca
    64561 tcagaaaatg gaaaaagctg ctgaaaacat ggagtttgaa aaagctgcag aataccgaga
    64621 ccagatccgt gccattgaga caatcatgac tcgccaaaaa atgacaaata ctgatttact
    64681 ggatcgagat gtatttggtt atgcgattga taaaggatgg atgtgtgtcc aagtgttttt
    64741 cgtaagacaa ggaaagctaa ttgaacgaga tgtttcaatg tttcctttct ataacgaagc
    64801 agaagaagat ttcttgactt acctcggtca gttttatcag gaaaatgagc atttcattcc
    64861 taaagaagtc ttgattcctg atacgatcga caagcccagt gtagaagctt tgttagctac
    64921 gaaagtgctg caaccaaaac gaggagaaaa gaaaaaatta gttgaattgg cgagtaagaa
    64981 tgcctcagtt gctttgaatg aacggtttga tctgattgcc agaaaacagg aacggacgat
    65041 cggagcagtt gaacgtttag gaaacgcgat gaatatcccg accccaatac gtatcgagtc
    65101 ttttgataac tctaatatca tggggacgaa tcctgtggct gcgatggtcg tattcattga
    65161 tggaaaaccg gcaagaaaag aatatcggaa atataaaata aaaacagtca acggaccgga
    65221 tgattatgca tcaatgagag aagtcatcta tcgcagatat tcgcgagtga ttcgcgaagg
    65281 gttgccttta cctgatttga tcttgatcga tggaggaaaa ggacaagtag atgtggcaaa
    65341 agatgtctta gaaaatcaat taggaatcga tattcctatt gctggcatgg caaaaaacga
    65401 taaacataaa acaaatgagt tgctattcgg tacagatttg gctgtagtac cgcttgagcg
    65461 aaattcacca gagtttttct tactccagcg aatccaagat gaagtccatc gattcgcaat
    65521 cactttccat cggcagctac gcagtaaaaa cagttttgct tcccgattag atgaaattga
    65581 agggttagga ccaaaacgaa aaaaaatgct gcttaaagag ttcaaatcgt tgaaaaatat
    65641 cacagcagcc acgacagaag aattacaaga aatcggactg ccgaaaaatg tcgcacaaaa
    65701 cgtatttgat aaattacatc aggaatgagg aaagggcact ttacaaagaa agtaaagtgt
    65761 cctatcattt tatgtacaac aaaaagagcg tttacgtttt gttggtaaat aataaatcgc
    65821 ttacgtctgg tgtctaagga tatatgtgat ttcttgaaga aggagtgaag aaatgttaga
    65881 agtcaaggat cttgttaaaa cttttgggaa ctacacagct gtcgatcatg tctctttcca
    65941 aatccccgat ggaaaaatca tggggctaat tggccaaaac ggggctggga agacaacgac
    66001 ttttcgtttg atcttggatt ttttgacaca agaccaagga gaagtgttat ggaatggcca
    66061 tcatcttggg gagaaagatt atgatgtcat cggttatctt ccggaggaac ggggacttta
    66121 tccgaaagta acgattcaag accaactgct ttattttgca gaacttagag ggaaatcaag
    66181 aaaagagatt gaaccaaaaa tcgacttttg gatggaaaaa tttcaggtaa aagggaaaaa
    66241 gacggataaa gtaaaatctt tgtctaaagg aaatcagcaa aaagtccagc tgatcgccac
    66301 gttaatccat gaacctaaat tgatcatttt ggatgagcca ttcagcgggt tagatccggt
    66361 aaatgctgaa ttgctaaaag atggaatatt ggaattgaaa aaacatggtt catgtgtcat
    66421 tttttctagc cacaatatgg ataatgtaga aaaaatctgc gatcatttga tcatgttgcg
    66481 aaacggaaaa atggttttga atggaaaagt acatgagatt cgtgaatcat ttggccgtac
    66541 gaagttattc ttggaatctg gtttaagcca acaggaagta gaagaaatcg aaggcgtgaa
    66601 aaaagtgatc cttcatgaag acaatacgtt agagattacc ttagaagatc ctgaagctgg
    66661 gaaagaaatc tttactcgtg cgactcagtt cggctacatt cctatgttta accagcaacc
    66721 gccaactttg gaagagattt tcaaattgaa agcaggtgaa atcgatgcgt aagttttgga
    66781 ttattaccaa agatgtctat ttaaaaaatg ttaagtcgat ttctttcttg atcatgatat
    66841 tagtcccttt tatcatgctg ggtatcattt atgtagcagg aaactttgct caacaaaata
    66901 gtgatgtcaa taaaatcggc gtattggctg ataatgagca aatcagtcag caaatgagcc
    66961 aagtcaaaac agatgatttt gagttcaaag cagttgattc tgaagaaaaa gcacaaaaag
    67021 agctttcgga agaaaaaatc gatgcgtatt tagtagtctc aactgagaat gataagataa
    67081 atgggatgct ttatagcgaa aattcattag gacaaagtac acaactactg atacaacaac
    67141 aactgactgg cttgcaatcg atgcttcgag cgagtcaatt aggcatttca ccagaagaag
    67201 tagctagctt gagccaacaa gcaagtttga ctcgacaaaa agtcaatttt gattcaaaag
    67261 gaaaaatgac agtcggagaa gataactcga cagtccaata cgtcgtaagt tatgtagcaa
    67321 cgattgtctt gttcattatt attttgacgt atgcgcagat cattgcacaa gagatcgctt
    67381 ctgaaaaagg cacgcggatc atggaagtga tcctgtccag tacgactgca caaaagcatt
    67441 tttacggaaa attaacaggt gttttacttg ttgcattaac gcaaatggca ttatacggta
    67501 ttatttttgg tataggatac agtcaattca aagatatgga aatggtcaaa aatatattag
    67561 acggtatctc attggataac ttgattggac cattcttgtg gtattctctt ctattcatga
    67621 tctttggcat tttgattttt tctgttttag cagctttatg tggctctttg gtcaacaaag
    67681 cagaagatac agcaaaagca attttgccag tgacttatct atctctaggc ggatacatgc
    67741 ttggattgat actaggagct tcagatccaa acaatatcgt tattcggatc acttcgtata
    67801 ttccattctt atcttcatat attatgccgg ttcgcttagc aaatgagaca gtcggtatgg
    67861 gtggagcatt gatttccttg ttggtacttg tagccgtaac attcttattg atgatcttgt
    67921 ctgcaaacat gtacaaatcg aatgtactcg tttatagcga aggcggatta tggagttcat
    67981 tgaaacaatc gatttcaatt atgaggaatg aacgaaaaaa agcataaaac aatcctaaaa
    68041 tacagaagga agctggacat ctattgttcc cagcttcttt ttgtatagac ctattttatt
    68101 tattttacta tattggatat atattcattg aaaatagtgt ataacaactt tataatattg
    68161 agtataccaa tttttggagg cgttttaatg gaccgttatt tagatttact ttcagaaaaa
    68221 ttcccctcaa cggaggatgt catcacggaa atcatcaatc tcgaagcaat tttgcagctt
    68281 ccaaaaggaa cagagttatt tttgagtgat atacatggcg aatttcctgc ttttgatcat
    68341 attctgcgta tcagttcagg aaatcttaaa gaaaagatca aagacctttt ttctgaacaa
    68401 atgacggaaa cggatatcag ccaattcact atttttacgg cttatccaga atatgcatta
    68461 acgactgatt ggtacaaaga acaagataaa tcgcagctga tccatcattt gatcgatctt
    68521 ttacggttta cgacagtcaa atatacccgg tcaaaagttc gaaaaggatt gccgaaagaa
    68581 tatagctata tcattgagga actcttgtat atcgatgacc gtatcaatgg aaaaaaagac
    68641 tatgtaaaaa aaatcattga acaacttgtt ttgatgaagg aagaagaaaa atttttaacg
    68701 aaacttgcac agacgatcca aaaatctgtc atcgatcatt tgcatatcgt tggagatatt
    68761 tttgaccgag gaactcaagc agccaaagtg atggatcgaa tcatggattt ttcttctgta
    68821 gatatccaat ggggaaatca tgatattttg tggattggtg cgtattgcgg ctctgaggca
    68881 tgtttactga cacttttacg gattgcagca cgctataatt atttatttga attagagaaa
    68941 gaatatgggt tgaatttacg tccattgttt tcttttgctc atcaaactta tcagaagaat
    69001 ccagtatttg cgcctaaaaa cagtaaaaat ctatccgaaa gagagcaaga agaattagaa
    69061 aaaatccatc aggcactttc tatccttcaa ttcaaatcag aacatcagtt attggatcgt
    69121 cgacctgaat ttaaaatgga cgatcgtaaa ttgctggaaa aaatcgacta tgaagcatca
    69181 acgattgatt tagaaggtca gctctatgag ttgaaaggaa cttgttttca aacgatccag
    69241 ccagatgatc caaaaaaatt cttggaagag gaacaaaaag tgattgatag tttgatgtat
    69301 tcattccagc actctttgag aatgcaaaaa catatgacgt ttttgatcga aaaaggggga
    69361 atgtatctca cgaacaatca gcatgtgctg tttcatggct gtatacctgt agacgagaag
    69421 ggacagctct tagcatttga attagaccaa tcttatcgcg gaaaagaact gctgggcttt
    69481 tttgaaaaac agataactga aagcgcaaaa gatctgactg caaaagaaga tttagcaacc
    69541 gatttgattt ggtatgcgtg gagtgggcca ttttcgcctt tattcggcaa gagtaaaatg
    69601 gcgacttttg aacgttattt tctcacagag cctcatacgc acgtcgagaa aagcaatcct
    69661 tattatcact tgcgagatga agagtggttc agtgaaaaaa ttttagcaga attcgacaca
    69721 aaagaagaag ggtcagcaat cattaatggg cacacgccag taaaagtaaa aaaaggggag
    69781 tcaccgatca aagcaaatgg aaaagtattt gtcattgacg gaggtttatc caaagcttat
    69841 caaaaaacga caggaattgc cggttattca ctattatgca attcttatgg tttccaaatc
    69901 gtgacacatt atccgttttt gcctattgaa caacaatttg caaaaaaatc cgaccaaaca
    69961 aatgtgaaaa aagtaatcga acagcaacta ccacgcaaac tcaatcgtga tacaacaaaa
    70021 ggtatggaac tgcagcagca gattacccag ctcaaaaggc tgttggacta tcgaaaggac
    70081 gattgagaag aattccagtg aacattgttc catagactgg agactgagac aaaagtcgtg
    70141 tcgcagtctc tttttccgga taaacggtgt tcttctgcca actcctcggt cacaagtcac
    70201 actattcaac aaacatgaaa agctgttttt tgaaattgct ccttgtgact cagagttgaa
    70261 cggcaggctc acgacctctt ggtataaacg gtgttcaaaa gccagtttct cggtattaag
    70321 ccaaatttat gcaaaaatgt gaaaagccat tttcacaaaa tttttcttaa tactcgaaac
    70381 taaacagctt tttcacaacc ccctcagaaa gtctattaaa attcattcac aaagaaatcg
    70441 aataagcgct gttcagatgg atgtacacgg tgggtaagtt ccggatgcca ttgaacaccg
    70501 aggattttat gcatatcatc tttgctttga accgcttcaa tgagtccatc gttagaaaaa
    70561 gcgatggctt ttaataaagg tgaaagatct ttgactgcct gatgatggta agaattcaca
    70621 aaatatggat cggggagaag gttgcttaac aaactgtctg aaacaatctt gacttcgtga
    70681 gtcgtaaatt gcggggcagt gggctgttgt tcatgtttta ctgaccactt tggatataat
    70741 gaaagatctt gataaagtgt accttccaaa acgacattca atagttggag tccccggcag
    70801 actgcaaaaa ttggttttct ttgtttcaat gcttccaaga tcagtgcctg ttcaaattgg
    70861 tctcgttgaa tatttgtttc cgttagcttt ggatgaggat cttccatgta aagttgtgga
    70921 ctgacatctt gaccacctgc aagaataagt ttgtcgattt gtccaatcag ttgaggagct
    70981 gaatctggag acatgactgg cagaataaga ggcaatcctt gagcatctaa aactgcatcc
    71041 acgaatcctt gcggtgtata gctcacttga tttccttgga aagtatctgt tgctcgaatc
    71101 agttgatttc cggcaatgcc gatgattggt ttcatttgta ttccccctaa agtagactta
    71161 ttgtaacaag gatttaaaaa aaattaaact atgtaagact tgcccctata cttatctgtt
    71221 cttgctgtgt tactatctgt aaagagaaat ttcgtgtgaa attcatgacg atagttgaaa
    71281 atattgctta atttgttata ataaaaagta agcagaaata aaaaggttgg gcgtaagctt
    71341 caaggatcta gttcttcaag gggtgagaag gacattttat tgaaggggcc tgcctatact
    71401 aagttatctt tagaggtaca tactttcaat gaaaaaagaa agcatgtacc tcttttttaa
    71461 atcgaaagga agtgaagcaa gtgttagaag ctttggaaca aatccgaaaa gcagaagaag
    71521 aaaatgaaaa gcgtgctcaa acactgcggc agcagctgaa gatctatgca acagaaaaag
    71581 agcaggaatt ggaaacgatc caaacaaact atcgacaaca actaaaacag ttggtggaag
    71641 aaaaagaaca atttgaaata caagaattag aaaaagaaaa aatgaaattg aaagcagaag
    71701 cagacaaggt gcaagcaact ttgaaagatc aatatcaaaa gcatcagagt caagcgatcg
    71761 gtgcgatcat tgaaagggtg aaagaaactt atggccgtca ctaagatgga aaaagtgacc
    71821 ctcatttcag ataaaagaaa tcaggagagg attttacaag cagtacaagg aatccaaaac
    71881 gttgaaatcc gggatctgta tcaaaataga acaaacaacc agtgggtgga gaagtatttt
    71941 ccagatacca cgattattga taaagaagca aaattaagcg ctttaggtaa tcggttgaaa
    72001 gacatccaag aagctatcca attcatacaa caccacggcg atagccgtca aaagaaactc
    72061 catttgaaac gtcaagaact ttccttacaa gatttggaag ccaattattc cgaagaaaat
    72121 ttcataaata aattgaaaga agtccttgat ttaaaagaaa agtgggaaca actaactgaa
    72181 gaacgtgatc gtttagaaga agaggaagaa tggcttcttc attggcaaca gctggatatt
    72241 gctccaagta tgtatgcaag taagacgacc atttttgaga tggggactgt gagtgcagca
    72301 aattttgaaa gtttcaagga agaactgagt aagcttgatg acgtttatat ggaagaaata
    72361 gattatacgc caaaacaagt ttatctttcg tatgttgctt taaaaaaatc agttgctgtt
    72421 atagaagaaa ttgctgggaa atacgggttc aataaagaaa cgtatcctta tgaacaaaat
    72481 cctaaagaaa aactgcaaga gaccaagcag caattatcca ttgttcacga gcagcaaaag
    72541 aagataacga cagcgttagg tcagtgcagt ggctatatcc aagaatttga atgggtagaa
    72601 gaagtcacac ttgccgttgc agaacgagaa aaaataaaag aacggtttat ccatgcgact
    72661 tatctgatcg ttttgcaagg ttgggtagat gcagaagaaa aacagaattt gctgcatgtg
    72721 ttaaacgaaa ccgtatcaga agaagatata tacgtcagtt tcgaaacacc aaactcagaa
    72781 gagattcagc aggaagtccc tactaaattg aaaaaccatc cgcttgtcga accttttgaa
    72841 ttgctgactg aaatgtacag tctgccaaaa tatgaagaag tagatccaac accttggatg
    72901 acaccatttt atcttgtctt cttcgggatg atggtcgcag atgttggtta tggtttgctg
    72961 atgctcattg ggacaatact agctcaaaaa ctacttgttc taccacgcgg gatgaaacgt
    73021 ttcgtaaaat tctttgagat tctctcgata ccatcgatca tctggggatt gatctacagt
    73081 tcatttttcg gacagtcatt accaaaagaa ctgtttggca tccgcttgcc tttcccaatc
    73141 ctatctacga cggatgacgt caatacgatt ttgattttat ccgtgatttt cggtctgata
    73201 cagattttgg ttggattatt tgtggcagca aaggaaaata tcaggagaaa agattacttg
    73261 agtgctgtca gtgatggatt tgcctggcaa gggatcctaa ttgggatcgt tattgcgttg
    73321 gttggtgcca tgttgataaa aaatacagca tttgtttacc ttggcgctgg gctatcgatc
    73381 attgctgcct tatgtatctt gatcgttccg attatccaaa cgccgtctaa agtcaaaggt
    73441 gctgccaaag gtgcgtataa tctttacgga ctgaccggct atatcggtga tttagtcagt
    73501 tatacacgtt tgatggcatt gggtatttca ggcggaagta ttggagcggc attcaatatg
    73561 ctagttgctt tcatgccgcc agctgctcga ttcagtgtag ggatcctttt gatcatcgct
    73621 ttacacgcac tgaatatgtt tttgacactg ctaagtgcct atgtccatgg cgcacgattg
    73681 cagtatgtcg aattttttgg gaagttttat acaggaggcg gacgtgcctt tgatccgctg
    73741 aaaacggcgg aaaaatacgt caacatcaat cacaagaaaa aaaacaataa ataattggag
    73801 gatttttata taatgatgga ttacttaatt agtcaaaatg gtggaatggt atttgcggta
    73861 ttagcgatgg caacagcgac gattttttca gggatcggtt cagctaaagg tgttggaatg
    73921 acaggtgagg cagcagcagc attgacaaca agtcaacccg aaaaattcgg gcaggctctg
    73981 attctgcaat tgcttccagg tacgcaaggt ttgtatggat tcgttattgc ctttttgatt
    74041 tttatcaact taggaaatga catgtcagtt gtacaaggac tgaactttct aggtgcttct
    74101 ctgccgattg cttttacagg cttgttttca ggtattgccc aaggaaaagt cgcagcggca
    74161 ggtattcaaa ttttagcgaa aaaacctgaa catgctacaa aaggaatcat ttttgctgcg
    74221 atggttgaaa catacgcgat tttaggtttc gttatttcct tcctattagt gttgaatgcc
    74281 taagcataaa ggaggaaaag cagatgaacg ccattgaaaa aatcatctct cagatgaacg
    74341 aagcagcaga gcaggaaaga gcggcgttag aacaagaaga gcgaatgaag atcgatcaaa
    74401 actttgaaca aaaacgtacg caagttgaaa cagaacatca aaaacaaaaa gaaaaacaaa
    74461 tcgaattact cgaaaaaaaa taccgtcagc taaggaatcg ccagcaagta gaagtacgac
    74521 aagaaaattt gaatgcgaag caggaatttt taagacgatt gtttgcagat gcggtaactg
    74581 aaatggaaaa ttgggatgaa tctgaacaaa tccagtttat caaaaatgca ttgtattcat
    74641 tacctttgac aggaaaagta gcattcattg ctggagaaaa atcagcagcc tatctttctc
    74701 agacgttgtt ggatgaatgg aacaatgaac ttccctttat gatggtttta agtgatgaaa
    74761 ctgttgctga tcaagcgggt tttctgataa acgatcaagg agttcagtat aattttcttt
    74821 tcagcagcct tgtacaagat atccaaggaa cgatgagttt tgagattgcc aatcaattat
    74881 tcgaataagg agggatcagt gtatggaata ccacgaacta aatcccttga ttcgcggaag
    74941 agagcttgaa cttttgtcca atgagacatt tgaacggatg attcaaacaa attctgtcga
    75001 agcacttggt gaaatattga aatccacgat ttacagtcca tatatttatg acggatatga
    75061 atatgacttc gaacagaatc ttttaaagga gcagagccgt ctatttcgct ggttaaagga
    75121 atcagcaccg gagcctgaaa tcgtttggat ctatacgatg cgttatactt tccataatct
    75181 gaaggttttg acaaaagctg agatgacaaa tcaaaatctg gaccatttgt atatcgacga
    75241 tggattttat tcattagaaa ccttgaagga tgccattcat acgcaagttt caaatgaact
    75301 tcctgaaagc attatggaca gtatacgaga agtacgtgat tattttgaag agtctaccat
    75361 tcttcaggga attgacgtga tttatgatcg acagtttttg acagagcagc gacggcttgc
    75421 cgataagcta ggatatcaag aattgctgac agaagtcatt gcttttattg acttgaccaa
    75481 tatcacaacg atggctcgag gaatattgca gaaaagaaca tatgggttca tgacgacggt
    75541 actttccagc tcgggaagta tcgaaaaaga agtatttttg aactttgtag ataaagatat
    75601 ggaagattac acgaattttc tattagagac agattatgct gattttctgc gccctgtcat
    75661 ccgtgaggga aatattgatc tcgtaaagct tgaacagttg aaagatgatt atctaagttc
    75721 attgtatcaa gaagcacaaa cccaggcttt cggaccactg ccactactag cctttttgaa
    75781 cgcaaaagag gtagaaagca aaaaccttcg tttactggtt atcggcaaaa gaagccattt
    75841 cgaatcagaa acaatcagaa agcgggtgag acaagtctat gacgcataag atcggtgtag
    75901 ttggcgacaa agattctgtt ttgccattca aattgtttgg ctttgatgtt cgttatggta
    75961 caaccaagca aaccgttcgt caggcaattg aagaaatggc aaagaatgag tatggcgtga
    76021 tttatgtcac agagcaatgt gcagcattga gcccagaaac gatcgaacgt tacaaggaac
    76081 aaatgatacc tgctatcgta ttgattccca atcatcaagg atcattaggg atagggctgg
    76141 aagaaattca aaaaaacgta gaaaaagcag taggtcaaaa tatcttatag tatgaaaagg
    76201 agatagaaat tttgcaaatt ggaaaaatca taaaagtctc cggacctctt gtaatggctg
    76261 agaacatgtc tgatgccagt atccaagaca tgtgtctagt gggagatcta ggagttatcg
    76321 gagaaattat cgagatgcgt ggcgatgtgg cgtctattca agtatatgaa gagacttctg
    76381 gaattggtcc aggcgaaccg gttcgctcta ctggtgaagc tttatctgtt gaattaggac
    76441 cagggatcat ttcacaaatg tttgacggaa tccaacggcc attagatacc tttatggaaa
    76501 tcacacaaag taatttctta gggcgcggag ttcaattgcc cgcattggac catgagaaaa
    76561 aatggtggtt cgaaccaaca gtagaagccg gagaaacggt gagtgctgga gatgttatcg
    76621 ggatcgtaga agaaacaaaa gttataaaac ataaaatcat ggtgccaaat gggataaaag
    76681 gaacgatcaa aacaatcgaa aaaggttcat tcactattga tgaaacagta tgtgtcattg
    76741 aaaccgaaaa tggtgacaaa aatttgacga tgcttcaaaa atggccagta agacgtagtc
    76801 ggccaatcaa agaaaaatta aacccggatg cacccatgat cactggacag cgagtgattg
    76861 atacattctt cccagtgaca aaaggaggag cagcagctgt acctggtcct tttggtgcag
    76921 ggaaaacagt cgttcagcat caaattgcaa aatgggccga tgtggacatg gttgtctatg
    76981 tgggatgtgg cgagcgtgga aatgagatga cggatgtttt aaatgaattc cctgaattga
    77041 ttgaccctaa tacaggagaa tcgttgatgg aacgtacggt attgattgct aatacatcaa
    77101 acatgccagt agcagctcga gaagcttcta tctatacggg aatcacaatt gcggaatatt
    77161 tccgtgacat gggttatgac gttgctatca tggcagactc cacttcacga tgggcagaag
    77221 cacttcgtga gatgagcgga cgtttggaag aaatgcctgg tgatgaaggt tatccagctt
    77281 atcttggttc acggcttgcg gaatactatg aacgatctgg aagagtcatt gcattaggat
    77341 cagaaggtcg cgaaggaagt ataaccgcaa tcagtgctgt atccccgtct ggaggggata
    77401 tttcggaacc agtcacacaa aatacattgc gggtcgtaaa agtattctgg ggtcttgatt
    77461 ccagtttagc ccaaaaaaga cattttcctt cgatcgattg gatacaaagc tattctcttt
    77521 attcaacaga agtagggaag tatatggatc atatccttca agctgactgg gcatccatgg
    77581 tcaccgaggg catgcgtatt ttgcaggaag aacagcagct gaatgaaatc gtgcgtttgg
    77641 tagggattga ttccctttca gataatgacc gtctgacatt agaagtggca aaatccattc
    77701 gtgaagatta tttgcagcaa aatgcttttg atgatgtcga tacattcact tcaagagaga
    77761 aacaattcaa aatgttgaag atgatcttga cctttggtga agaaggacgc cgtgcgttgt
    77821 cgttaggtgc ttatttaagt gagatcatgg acggtaccgt atcgattcgt gaaaggatcg
    77881 gacgaagcaa atatatcccg gaaacagaat tagaaaaact agatcaaatt attggtgaaa
    77941 tcaagcaaac gatgcaaacg atcgtttcag aaggaggcat gagcaatgat taaagaatat
    78001 cgtacgatcg gtgaagtcgt cggtccattg atggcggttg aaaaagttgc tggtgtcaaa
    78061 tatgaagaat taatcgaagt ccgtatgcaa aatggcgaga ttcgtcgtgg acaagtattg
    78121 gaagtccaag aagataaagc aatggtccaa atattcgaag gaacaagtgg tatcaattta
    78181 cgtgactctt ctgtacgatt tttaggccat cctttagaac taggtgtatc ggaagacatg
    78241 atcggtcgag tattcgatgg tttgggacga ccgaaagata acggaccaga gatcctgcct
    78301 gaaaagcatt tggatattaa cggagaagta atcaatccag tagctcggga ttatccagat
    78361 gagtttatcc aaacaggtat ctctgcaatc gatcatttga atacattagt tcgtgggcaa
    78421 aaattaccag tattttctgg ctctggtctt cctcataaag aattagctgc tcagatcgcg
    78481 cgacaagcaa cggtactcga ttcatcagat gatttcgccg tagtctttgc tgctattgga
    78541 atcacttttg aagaagctga atttttcatg gaagattttc gtcaaacggg cgcaatcgac
    78601 cgttctgtca tgtttatgaa tttagcaaac gaccctgcaa tcgaacgtat cgctactcct
    78661 cgtatggctt tgactgctgc agaatatctt gcttatgaaa agggaatgca tgttttggtt
    78721 atcatgacag acatgaccaa ctatgcagag gcattgcgag agatttctgc agcacggcga
    78781 gaagttccag gaagaagagg atatcctggg tatctttaca cgaacttagc tactttattc
    78841 gaacgagcag gaagaatccg cggactaaaa ggatcggtta ctcaaattcc gattctaaca
    78901 atgccagaag acgataaaac tcatccaatc cctgacttga ctggttatat tacagaagga
    78961 cagatcatct tgactagaga gctttacaag agtgggattc agccaccaat tgatgtactc
    79021 ccgtctcttt cgcggttaaa agataaggga actggacccg gaaagacaag agaagaccat
    79081 gcagctacga tgaatcagct ttttgcagcc tatgcacaag gaaaacaggc aaaagaatta
    79141 gccgtagttt taggtgaatc tgctttatct gatgtagaca agatctatgc gaagtttgct
    79201 gagcggtttg aaaaagaata tgtcaatcaa ggattctaca cgaaccgttc gattactgaa
    79261 acacttgatc tagggtggga actgctatcg atgttgccaa gaaccgaatt aaaacgaatc
    79321 aaagacgaca tgctggacaa atatctgcca gaagggaagt gagttctgat ggcacgattg
    79381 aatgtgaatc cgacccgcat ggaattgact cgattgaaaa aacagttgac aactgccaca
    79441 agagggcata aattattaaa agataagcaa gatgaactga tgcgtcaatt catcctgttg
    79501 attcgaaaga acaaccagct gcgtcaagaa atggaagagt ctatgacaga agcaatgtct
    79561 gattttgtat tggcaaatgc ttcgatcacg gaacctttca tcgaagaatt atttgttttg
    79621 ccggcggaaa acgttgattt gtctatcgta gacaaaaata tcatgagtgt aaaagtacct
    79681 gtgatgaatt ttgaatatga tgaacagctg acagacacac ctttggaata cgggtatttg
    79741 aattccagtg ccgaactgga tcggtcaatc gatcggttta ctaccctgct tccaaaactt
    79801 ttggaactga cagaaatcga aaaaacttgt caactgatgg cgagtgaaat cgaaaagaca
    79861 cgtcggcgag tcaatgcatt agaatatatg acgatccctc aattagaaga aacgatctat
    79921 tatattcgta tgaagctgga agaaaacgag cgcgcagaag taacaaggtt gatcaaagta
    79981 aaaaatatgg gtaccactga ctaagtggta tcttttttta aaagaggaaa acgatgatta
    80041 aacaaagcaa acaaataaag aaacgtttgc cggctgttca gcttctcgca ttaggatttt
    80101 ttctcctgat tttagttggc ggcggcctac ttaccctacc attttttagt aatagtgggc
    80161 atgggacaaa ttttatcgat gcgcttttca ctgcaacttc tgctgtctgt gtaacgggat
    80221 tgacaacatt gaatacagca gagcattgga acagtgctgg tcaatttttg atcatgcttt
    80281 tgattgagat cggcggtctt ggttttatga tggttccgat tctatgcttt gcattgataa
    80341 agaagaaagt cagtctcagc acacgtatta tattaaaaga agcgctgaat ctagaagaag
    80401 tatcaggtgt gatgaagctc atgctttaca tcctaaaatt ggcctttgtg atccaattga
    80461 ttggtacggt tgctttatct tttgtatttg tgcctgaatt tggctggttg aaaggtatct
    80521 ggtacagttt gttccatgca atctctagtt tttgtaatgc agggtttgat ttgttaggag
    80581 acagtttagc caatcaccag caaaatgttt atttgatcat ggtggtatct gctttgatca
    80641 tttctggtgg gttaggcttc atcgtatggc gtgatttact ggaatatcat cagaaaaaga
    80701 agatgacgat tcattcaaaa atcgcattaa ccgttacagg taccctttta ttaggcggat
    80761 tcgttgtgtt ctttttcaca gagcgcaatg gggcaacgct agtggaaggg aatatgtttc
    80821 aacgattggc aaacaccttt tttatgagtg tgacacctag aacggctgga tattattcag
    80881 ttgattacct ccatatgagc catgcaggtc tgattttaac gatgtttttg atgtacatcg
    80941 gcggaacatc tgggtcgacg gccggcggat tgaaaacaac tacgttaggt gtgttgctga
    81001 tccagatgcg ttcgatgttg aaaggacgta cgagagcgga agcatttggc cgtaccatcc
    81061 gacaaggcgc agtgttgcgg gcacttactt tattttttgt tacgttgacg ttgtgtgtag
    81121 tcgccatcat gattttatcg gtgactgaaa cgatacctaa aacttctggc atcgaatata
    81181 ttgcttttga ggtcttctct gcgtttggta cagtaggtct tacgatggga ttgacgccag
    81241 acttgactgc aatagggaaa ttgatcatta tagcgcttat gtacattgga cgagttggtg
    81301 tattgactgt ggtcttctct ctgatgacaa aagcacaaag aacacaatca aaatacaaat
    81361 atccagaaga aagtgtcatg attggataat ttcatcttct gacagaaacc tagtgattta
    81421 gtaaaataag aaaataaaat aatttttctt gcttcttgga tactttaagt gcaaaatgtt
    81481 cgtttatttt ttgaggcact acccgttgtt gaacctcggg tagtgcctct tttttattga
    81541 aaggaatcaa taagatttga ttcctttatt tatcaatagg ttataattca tattttttta
    81601 ttttttcttt tacgatactt agtccagaag ctaaaatttc aaggttatta gaggataggg
    81661 aaattcgaac gaatgcatct ggttgcgtac gtgtagtaaa gcgtctggaa tgataaagtc
    81721 ggataccgtt agcaagaaag tcattctcaa ttatcttagg gtcaatctca ggatcaactg
    81781 gaagaattcg atagtaaggg acacgattcg ttggtttttc tactttgaaa agagaatcaa
    81841 aaagctgatt ggctcttttt gtaagactta atttagtttc taagatccta tttgcttcat
    81901 ttgaatggag gatttgagtg atgacttcca catctaatcc agaagttttc acatttatat
    81961 taaaaattgc ttgttctatc ttgctttgga catgtactgg aactacaaga taagcaactc
    82021 gcaatccaga acaaataaat tttgtcattc cagctaagta gattgtttgg ttaggtaata
    82081 aattttggaa aggaggaatg atcgtatgtt gatagtaagt tgttaaaaat gaatggatat
    82141 catcttcaat aacccataga ccttcttttt caatcacttc ggctaattgc tctcgtcttt
    82201 tgtgagagat ttgaaaccct ataggattat tacaagaagg cataagaaag acaccatgaa
    82261 tcttcttttt tctacattcc tgaatcaata tatcaggtcg cattccttgc tcgtcaaaat
    82321 caattggaac gatctctaaa tgatataact gagccaattc aatgaaatta gcgtatgtat
    82381 atcggtctgt tgctattcta tcacctggag agaaaagtgc agaaagtgtg atagccaatc
    82441 cattttgaac accggaaaca atagatagag attctaagga cggaatgact ccttgagttt
    82501 ttaaccaatt tgctgcagta gtcaattgaa aatttgtgcc tttgggatct tggtaattaa
    82561 gcaaaccaac aagatttggg ttattactag ttgattgaat aaacgggaca acgaatttat
    82621 tacattcttc aaaagaacta accagaccaa aatcaatgat atgttcggtt acttcattgg
    82681 tagagatggt gatggactca aagacattgg gagaaataaa tgtgccactt ccaatattcg
    82741 tatacagcaa acctttctca attccatatt tataagcctt gccaacggta gtaaaattta
    82801 aatctaaata atcagctagc tctctttggg agggtaactg agtgtttttc tgtaatttat
    82861 tctttaaaat atcttgttct aattgttgaa tcaacgatcg ataataagga cgcttcaatt
    82921 tttgtttgtc tggtcgccaa gttaacgggt aattgtcata agaattaata ggcattttct
    82981 ccactcctta aaattgtata ccatacaatt ttaccattga ttgtattgat tttagaagtt
    83041 aatcttttgt gaggagggaa ttgtctatga aaattattaa ttttgaggaa gtagtagctt
    83101 cttttagttt tgaagaagcg attcaagtaa tgaaaaaatg tttttttgat tatgaaagtg
    83161 gtaaaatcag ccaaaatcct cgattggtgg aaattttacc tgacggaaaa ggcaaaaata
    83221 tgtttgcgat gatgcctgct tatttggggg agaacagata ttttggcaca aaagtcatta
    83281 ccgcttttcc ggataatcat cagtatcatt taccttctca tttaggagag attttcttgt
    83341 ttgatgctca aaacggacta cccaaagcat tgataaacgc aaatgcagtc acctggattc
    83401 gaacagctgc tgtatctgcg ctggcaacag attacttagc aaatccgcat gcagaaacac
    83461 ttgctttaat tggagctggt caacaagcat tgtcgcattt gactgcgatt tctaccattc
    83521 gtcccatcac tacagtgttt gtttacgata ttttagaaga aagacgaaat acttttatcg
    83581 ctaaagctaa aaaacagtat cctaaaataa cgtttataaa tgcaatatca gtagaagatg
    83641 cagtacgtcc agcggaaatc atttgtacgt tgacctctag taaagaggca tttcttcaaa
    83701 aagagtgggt aagggaagac gcacatatca atgctgtggg cacatttact cctgatacaa
    83761 gagagataag tacatcgttg atggaaactt ctcaaatcta tgtagatgat tactcagctg
    83821 ccttaacaga aagcggggac ctgcttatag ccatggcaga aggagattta gaggaatctt
    83881 gtattgttgg tacattaggc gaattagttt ctgagaaaaa aacattttat aaaggagaat
    83941 cgaggccgac gattttcgac gcagttggtc tagctattga agatctttgt tgtgcagaat
    84001 atatttataa caaattagag aaaggggctt taaaatgaac ttagattatt acgtagttga
    84061 tgcatttgca gatgaggtgt tcaaaggaaa tccagcagcg gtttatgtgc tagaagaatg
    84121 gctgccagaa ggtacgatgc aaaaaattgc cattgaaaac aatttatctg aaacagcatt
    84181 tacagtgaag aaaaaccaag aatttgagct tagatggttt acgccagatc gagaaatcga
    84241 tctttgtggt cacgcgacat tagcaacagc atttgtttta tttaactatt acaaaatacc
    84301 tgatgaaacg attaaatttt ctactcaaag cggtaatcta tttgtgacaa gaattaaaga
    84361 ttattattac atggattttc ctagtattat gccgaaaaaa gtgccgatat tgtctgaata
    84421 tgaagaagcg attggggcta agattaaaga agcctactta gcacgtgatt tatttttcgt
    84481 attagaagat gaaaaaacgg tggctaaatt gcagccagat tttacagcta tcaaaaattt
    84541 tgaattagga ataggagtca tcgtgacagc agaaagtgtg caaaaagact ttgtttctag
    84601 aaccttcttt ccaaaattaa caatcaatga agatccagta tgtggatcag cacattctaa
    84661 tcttattcct tattgggcag aaagaatgaa taaaacaaaa ttgtcagctt atcaaatgtc
    84721 atcaaggggc ggggatctag aatgtgaatt aaaagaagat cgtgtgataa taggaggaaa
    84781 agcagttttg ttttctaaag gtacctcgtt tatttgattt ctttcaaatg aattaaaaat
    84841 aaattggtgg agagtggcaa ccttatagct acaatggttg ttaaaaaaag acgcaaactt
    84901 tttcaattta atgaaaagtt tgcgtctcat tttaattatt ggtgtttgtt ttttattcaa
    84961 tcaagagtaa aatgccccat actaactatc ctcggatgac ttcgatttta taaccatcag
    85021 ggtcaatgac aaagtagtaa gaaggcgcag tgccaggtaa tccttttaag tcagtaatct
    85081 caagaccagc agctttatgt tgctcgtgta atttttctag atcatcagct gctatagcga
    85141 tatgaccata gccgtctcct agatcatacg caccatgatc atagttgtaa gtcaactcta
    85201 attcatagtc atccccagga agtgtcaaat aaacaagtgt aaatttgttt tctggaaaat
    85261 cacggcggcg gctttcttca aaaccaaatg ccttttgata aaattcgatt gatgcttcaa
    85321 gatctttcac tcgaacacat gtgtgagcca ttttcataga ttcatctctc cttttccttt
    85381 tactcaatca taacaaaaag aagtaaggaa atagttgatt tgtgcttgtt catgagaaat
    85441 ttctcatgta aaatggatag aaaataaaac tgttaaggtg gaaactattc atggataaac
    85501 caatttttgg tgaaaaaaag caatttgctg attatcaaac ccgttatgca gcatatattg
    85561 tcatagaaag agatcagcag attgcagtaa tcgaggctcc aaatggtgct ttttttcttc
    85621 ctggcgggga aattgaagga gaagagacga aagaagaagc gattgtccgt gagctgatag
    85681 aagaaatggg aattgtggca gaaatttctt cttatctggg agaagctgat gagtattttt
    85741 actcgaatta tcgtgcaact tattattata atccaggata tttctttgtt gctgattctt
    85801 ggaagcgtat aggagaacca actgaaaaga ccaataaaat ttggtgggtg agccctcaag
    85861 aagcgcttgt gaaattgaaa cgtggtagcc accgatgggc agtcgagaga tggatcgaaa
    85921 aaaataaaaa ctgaaagaaa gcgagtagat cagctttctc tcagtttttt tatttattac
    85981 agtacatatt ttttgattgc ttcagctaca ccgtgctcgt cgtttgatga agtgatgaca
    86041 tttgctgctt ctttgacaag agaaacggca tttcccattg ctactcctag accagcatat
    86101 tcaatcatgg ataagtcgtt ctcgttatct ccgatagcca tgatttcatc ttgatcaata
    86161 ccaaggtgct gtgcgagatt cgcaactgcc gcacctttac ttgcttcttt atttagtact
    86221 tcgaagtaat agggcgcact tttgaccgtt gtgtatttat cacggaaagt ttgcggtaaa
    86281 cgcgcaattg ctgcatctaa aatttctggt tcgtcgatca tcatcatttt gacgatgttc
    86341 atgtctggtg tcatttcttc aggtgttcga tattttagcg gcatcttgac taaacttgct
    86401 tcgtggatcg tataagcgct gatatctcga ttaggcgtgt agatcgcttt gtcatcgatc
    86461 gaatggagat gactgccgac acggcgtgcc atcacttcga tctctaggaa atcttcatgt
    86521 gtcagtccgt gacgggcgat gattttacct gatttcgttt gttgtacgag tgccccgtta
    86581 taagtaataa cgtaatcatt atcttgaaaa aggttcaact cgtttaactg ttcctggaca
    86641 cctggtaagg gacggccagt gcataaaacg atcgaaacac cttttgcaac agcagcggca
    86701 atcgcttctt ttacttcttt tgttacttct cttttctcat ttaataaagt accatcgatg
    86761 tcgatagcga ctagtttgat agacataatt tcctccaata tattttaatt tatttcaatc
    86821 aatgcgccat tacggatatg gctggcaaat tcttgataag tttcgtcaaa taaatcataa
    86881 tgatcacgca aggacggatc aaccatttct tttggaaaga aaaatcgctc atctccttgt
    86941 tcttgtcccg ctaatgcagc aacaagctga ctaacctgtg ataattctat caaagttccg
    87001 tcatttcgca aaatctcgat ttgtgtgcga tgtctgcctt gttctgggcg ataaaaatca
    87061 taaggtagat cgtagctaga attcacagca gtgtaataaa caggattata tccgaccttt
    87121 tctactaaca aagtgagttc ttggactaat tttgaatccc ggttgccaga aaaagtagca
    87181 gatttcagtg gtttacgatt caaaaaacgt ttagctaaat catttaaaat aggatcagca
    87241 gattcagacc attgagtaaa gtaagtgccc aagaccccat catcaagttt gaggtattct
    87301 tctaaagtaa agcttccgtc aagaaatggc agaagtaatt gagaatggag ttcaaaactg
    87361 ctggcatctt cttgatacaa ctcgtgtgcg cgatgaagca gatgctccaa aattacttcc
    87421 ataccacggg aaacaggatg gaagtacact tgcacataca tttgatatcg gctaacgata
    87481 taatcttcca ctgcatgcat gccactcatg gaaaaagcga tcccgccttc ataaggacga
    87541 atcacacgta atattcgggt aagatcaaat gttccatatt ctgttccagt gaaataagca
    87601 tcccgaagca aatagtccat tcgatctgca tcgatctgac tagaaatcat ttggacaact
    87661 tgtgggttgg ggtaagtttt ttggatcaca ctagctacct tttcaggaaa accttcttct
    87721 actcggttaa gaatctgata cacttctgtt tctggagaag tgatgatctc tacagtgatg
    87781 gcttcatgat tcgtatggaa gatatgttca aatgtatgag agtaaggacc atgaccgaca
    87841 tcatgcaaca gtgccgcaca taaagtcacc agccgttcct gatcatccca gcctccgttg
    87901 cctatttttt cttttgaaaa atttcgagaa aagatatcgc agattcgtct tgaaatctca
    87961 taaacgccta atgaatgagt aaaacggcta tgttctgctc cgtgaaaagt gaaagaagaa
    88021 gtgcctaatt gtttaatgcg tcgtagacgt tggacttctt tcgaattgat caaatctaag
    88081 attacttgat gctggacatg gatatagtta tggactggat cacgaaaaac tttttccata
    88141 ggcagcattt gatatttata aggggttgtc ataatgtagc tcctttatgt tattacgttg
    88201 ttccattctc tggatttggt tgttccattg ctccagctgg atttcttttt ttagccagtc
    88261 ggtagaatcc tgtaaatcac ttgcagaata cagcttttca aaagcttttg caaatgcttc
    88321 tttgacttct tttacaaaaa ggggttggtc aagcagttct tcaagtgttg ccattgtagc
    88381 aggatccact gcaggatagc catttgtacc aaagtcttct tttaaagcag attgataaaa
    88441 tcgcttcatg acttgcccgc gtttttcctg attaccgttg acacttaagt acatcataac
    88501 agaaatccca tttttgatcc gacgctgagc gataccggca aattttttgc catggatgct
    88561 taaatcataa gttccaggac aataagaatg ggtgatttcg aatgcttgta tcggtgcctt
    88621 tggaaaagcg tatttagtca ataggagcat ttgctcgtaa gcgtcatcta tggttatttt
    88681 ttttgtgtca ctttggggga aaatcaaaga aatattcaaa attccttcat ctgatacgat
    88741 ccctaatcca cctgaatttc tgatcactgg agcgtaacct gcatctttca gtgtttggat
    88801 cccgtcactt agaaaaggag ttcgaacatc cttcatacct aagatgactg tgcagtcata
    88861 ttgccagaaa tgtaaaaaga tttgctggtg ttctttagaa tattccgtta atatatctgt
    88921 taatgcaaaa ggggaaaagt cgtttttttg ataaatgtgc tgatctagtc gaatcgcagg
    88981 gttcattcaa ccggctcctt tgtataaatt catattttct acaatattat actctaaaat
    89041 caaagagttg ttttggaaaa aaggttttct gtaaaaatgg acaagttggt tgacaaaaca
    89101 aaagaaacta ggaacaatgt agctatgaga agtaaaggag aatgaataat ggaattatca
    89161 caaggaacac cgatttcaat caaattgcgt acaaaagtaa aacagcaagg ggaagtgcaa
    89221 gatttttatt ttgatttgaa agggcaaatg gtgacaattg gcgatacgct gtacatacgt
    89281 tataaagaga ttcaggagga gacgggcgat gagattcctg taacgatcaa attagttcca
    89341 gatggtcatg tacagctgat ccgtgcagga gaaatgcgga tgcgtttgaa atttggctat
    89401 aaagaacggt tagaaacatc ctatcgaaca ccatacggta tgatccagat tgctactttt
    89461 acgaaagaac tgcatgttag tttgaaagac cgtcccacag cagggaaggt tcgaatcgat
    89521 tatgatctgt tcatgggacc ggaaaaaatc ggcgaatatt atttgacctt ggattttaca
    89581 gcgtaaatat aaaagaaaag tttgattttt tgtgtctgat tttgtatgat aaggagtaga
    89641 ctgtgaaagg acgtgtcctg attggaaatt agtgtatttg aaggtgcaaa caaaagcgaa
    89701 ttatcaatga tcgaagtagc acacgcgatc ttagaacaac gtggcgatgt catggatttt
    89761 tctgatttag ctaaccagat ccaaaactat cttgggaaat cagacagtga aattcgtgat
    89821 tcactcgcac aattttatac tgacttgaat attgacggca gctttatctc attaggggat
    89881 aatcgttggg gcttgcgttc atggtatgct atcgattcga tcgatgaaga agtaaaccat
    89941 ggaatggacg aagaggatga agatactcca cgtcgccgta aacgcaagaa agtcaacgcc
    90001 tttatcaatg atgatgaaga cgcgatcgat tacaatgacg atgacccaga agatacagat
    90061 ctgacagaag aagatgacga tgatctgttt gatgacgatg atgatgaaga tgaagaaatc
    90121 gctgcttata attctgactt gcaagaaatc ggagcagatg acccgacaga tgatgaagaa
    90181 gacttgccag gaaatatcga agaagattta agtatcatag acgaagacga tgatgatgaa
    90241 gattatgaag aggaagaatt ttctgactct gaaaaagaat aaacattatt ttcttacgag
    90301 aggttgtgac agagacgatc agcctatcag aataaggagg tgtgacacga tttttgtcac
    90361 accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa aaattgaaac
    90421 ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga atgattttta
    90481 ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat ggactcatcg
    90541 aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat aaagaagagg
    90601 ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa aaagaaaaag
    90661 agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca ttagatagtt
    90721 ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc ccccaacagt
    90781 tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata gacagtccgg
    90841 aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa attgcgacta
    90901 tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg aatgctttac
    90961 gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga aaatttaaaa
    91021 aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga ggagatccgc
    91081 cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa gagacgtctc
    91141 caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct tctttttaat
    91201 agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat tgaattgatc
    91261 ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc gaagatcaat
    91321 acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata ctttcgcgaa
    91381 ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac ggatcattac
    91441 caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt gattgaaagg
    91501 cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac aatcgacaag
    91561 aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta tctatctgtt
    91621 ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc atctaagtac
    91681 tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact tgttacgcct
    91741 acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc tcaatacgaa
    91801 attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt gacaaaaacg
    91861 attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga ctagcagttt
    91921 caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga agagatatag
    91981 tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg tttttcgaaa
    92041 ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct tcggagcatt
    92101 cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca actgaaacag
    92161 ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag tcatcacaat
    92221 caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc gcaatcagct
    92281 ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta aaagaaaaaa
    92341 acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct gaaatcgtga
    92401 agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat cagaatctgg
    92461 ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact gagatcaaaa
    92521 aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc acgaatgtag
    92581 gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa gattggaaga
    92641 caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat ggggcaatta
    92701 ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg atcgattatc
    92761 tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat ccaacggctg
    92821 tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga caagtatctt
    92881 taataaataa cacattttaa taagtagttg aacacataac taccttactt gcaaaggctt
    92941 aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg cggacagttt
    93001 tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag tagaacgcgt
    93061 cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc cactacacta
    93121 aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag gggattggac
    93181 aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca gaaactagcc
    93241 atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga ggagctgttg
    93301 gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga gccggaatgg
    93361 cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac aaattaccta
    93421 aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca agtccatttg
    93481 aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata catcgatttg
    93541 aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc tacgcctatc
    93601 caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt aggtgcacgt
    93661 agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc ggaattagct
    93721 tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa agaaatcgca
    93781 caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt acaaacgttg
    93841 agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat tcctgatgtc
    93901 cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa aaatcagcca
    93961 aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga taaagaaact
    94021 gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac gatcctacaa
    94081 cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa agctttcttt
    94141 atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac tgagatagat
    94201 gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac agacttcggt
    94261 gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac agaacagaca
    94321 gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga tcaagaagac
    94381 aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga tgagatagcg
    94441 atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag ttcatcaaaa
    94501 agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca ctttttctag
    94561 tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag gttgagattt
    94621 ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata tgggtcgttt
    94681 taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg atgaaataaa
    94741 aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct ataaaatttt
    94801 acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa gtatggggat
    94861 cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt tgatcgacac
    94921 ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa gccttgaaaa
    94981 cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag aatattttgg
    95041 taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag tggctgatgc
    95101 attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg agaatatcat
    95161 cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta atctggaaaa
    95221 taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag cgataactgt
    95281 acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc accgtgcact
    95341 ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg agccatctat
    95401 ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta ttgggcgtgc
    95461 attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc tgaaacaagc
    95521 aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag tcgtttataa
    95581 ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca tcatggcaat
    95641 cgcgggcgga aaatcaaaag ctaaagcaat tcgagcttat atgaaaaatg caccaaaaca
    95701 aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg taacccttta
    95761 aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt aaagtaggta
    95821 ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat gtagatggaa
    95881 tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac ttgttaaaat
    95941 acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt tcattcaacg
    96001 taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta ccatggggcg
    96061 aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa tcagcagctg
    96121 aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc ggaaacgatg
    96181 ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa acagttatct
    96241 caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg aacgacaaat
    96301 ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac caaatgactc
    96361 tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct gcaaacatcg
    96421 ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa ttgaacggta
    96481 aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca gaattagtaa
    96541 cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa gaagcttcaa
    96601 acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt ggtatgactt
    96661 acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac aaacaattag
    96721 ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta gtacgtactt
    96781 tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta tagacatgta
    96841 catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata agtttattca
    96901 ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga caaaaaagtt
    96961 cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga cgacactcgt
    97021 atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa agcaatcctt
    97081 ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc tttagctcca
    97141 gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc tgaaactcgc
    97201 ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt cgtattcgaa
    97261 aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac agaactaggt
    97321 aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac agctcaccgt
    97381 gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc tggattcttg
    97441 atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg accaatgatt
    97501 gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa cttgattcct
    97561 aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga agcaaaagga
    97621 atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa agaattaatc
    97681 gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc agaattttca
    97741 aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat ggctttagac
    97801 atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa aacagttgtt
    97861 tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac aatcggtgtt
    97921 tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg agactcagcc
    97981 gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac aggtggcggc
    98041 gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat caacgataaa
    98101 taaacgacca agtttgctca attctgacga gtaatcgaac aaaaggtgaa gggagttttc
    98161 atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc gtaaacctat
    98221 tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat ttgctgaagc
    98281 agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat ctcctgcatt
    98341 attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct cagcacaaaa
    98401 ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag ctcttgctga
    98461 tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt tccatgaaac
    98521 agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga caccaatcct
    98581 atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat ggatcgaagg
    98641 acaaattaca gctggtttag ctgatctttc agctgaacag gtgtcaaaca tggttattgc
    98701 ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa tcgctgacga
    98761 aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag tatcagaagc
    98821 agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat acatggcaaa
    98881 agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt cattcttgtc
    98941 attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga aatgtttgca
    99001 ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa aaaacaaact
    99061 caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag atcttagact
    99121 cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct tttggccgcg
    99181 gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta cgtgacggag
    99241 acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta aacaacatca
    99301 ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac aaagctatga
    99361 tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt ttaggtgttt
    99421 ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac cactacctag
    99481 gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac ggcggatcac
    99541 atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc gctccaacat
    99601 tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca atcttgaaag
    99661 ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac cttggttcaa
    99721 acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat gtacctggta
    99781 aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa gaaaaaggtg
    99841 tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg atcaaattct
    99901 acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta gacgaaaacg
    99961 actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa ttagttggtg
   100021 acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa ggaattgcta
   100081 actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc gaagctatcg
   100141 aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt gaaacagaag
   100201 attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa actggttctc
   100261 tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa gaccaacttg
   100321 gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac aaataattct
   100381 tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc acttagttaa
   100441 gtaatcgaac tctctacttg ctgaaatatc agcaggtaga gagttttttt gcaactagct
   100501 tctgtatatg atgtttacaa agaatggtac aagaaaagat catccttgaa aaaggaaagc
   100561 taacaataac tagcatcata taagagcttt taaggataga acagtagaat aggaatctat
   100621 gagcaagtcc tctcgtataa ataaaatagg agggaagtag cagcatattt ttcataaagt
   100681 ggctctcttt cttcattttt actttggttg ctcctatttg aaaattcgag cacactcaaa
   100741 tacaataaaa agaaatggca agggagagga ttagatgaaa aaagaagaaa aaatcgcgat
   100801 tttacaagag atcatccgaa ttaaatcagt gaatggaaac gaaggtgaag tagcagccta
   100861 tttgaataag cttttggcaa agcatgacat aacaggagag attgtttctt acagagatgg
   100921 tagagataat ttgattgctc gttaccaaaa gggccaatca ggaaaagtgt taggcctatc
   100981 tgggcatatg gatgtcgtgg cggctggtga tgaatcgtct tggacctatg caccctttgc
   101041 agcagaaatc catgggaacc gtttgtacgg acgtggtgct acagatatga agagtggctt
   101101 ggcagcaatg gtgattgcca tgatcgaact gaaagagtca ggaaaaccat ttaacggaac
   101161 agtcaaatta ttagccactg tcggagaaga agtgggtgaa ctaggtgggg aacagctcac
   101221 gaaagcaggc tatgtagatg acttagatgc cttgatcatc ggagagccaa ccaattacag
   101281 tttgatgtat acgcacatgg gatcaatcaa ctatacagtc acctcgcacg gaaaagaagc
   101341 gcatagctct atgccagacc aaggttataa tgcaatcaat cacctaaatg aattcattac
   101401 gaaagcaaat gctgaaatga atcacctcgc tgaaacaatc gagaatcctg tcctaggaaa
   101461 aacaatccat aatgttacat tgatcagtgg cgggaatcaa gtcaacagca tcccaagcca
   101521 tgcgcagctc caagggaaca ttcgatcgat tccagaatac ccaaatgata aaatcattgc
   101581 attgctgcaa tcaatcgtca atgaattgaa tcaagagaca gactatcatt tggaattgat
   101641 gatcgattac aacaaaatac ctgtcaaagc tgatccagat tctcctttga ttcattgcat
   101701 tcagcaacaa ttcagccagc cattaccttt agttggagcg gcagcaacaa cggatgcagc
   101761 cgaatttacg aaagctaacc attccttcga ctttgtcgtc tttgggcctg gtgtcgttac
   101821 tttgcctcat caagtagatg agtatgttga aatcgataat tatttggata tgatcgaaaa
   101881 atatcaagga attattttat cctacttagc gtaagatttt tttaataaat gaaaaaacag
   101941 agccagatta gcatta
//
LOCUS       DANKEF010000003        87683 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000003 DANKEF010000000
VERSION     DANKEF010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 87683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 87683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..87683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.003"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(11..616)
                     /gene="dhaL"
                     /locus_tag="PWZ48_000216"
     CDS             complement(11..616)
                     /gene="dhaL"
                     /locus_tag="PWZ48_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HDL2259909.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="PWZ48_000217"
     CDS             complement(644..1654)
                     /gene="dhaK"
                     /locus_tag="PWZ48_000217"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDL2259910.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(1629..2075)
                     /gene="dhaM"
                     /locus_tag="PWZ48_000218"
     CDS             complement(1629..2075)
                     /gene="dhaM"
                     /locus_tag="PWZ48_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HDL2259911.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            2178..2498
                     /locus_tag="PWZ48_000219"
     CDS             2178..2498
                     /locus_tag="PWZ48_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDL2259912.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(2610..3479)
                     /locus_tag="PWZ48_000220"
     CDS             complement(2610..3479)
                     /locus_tag="PWZ48_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2259913.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(3575..4879)
                     /locus_tag="PWZ48_000221"
     CDS             complement(3575..4879)
                     /locus_tag="PWZ48_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2259914.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEYTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            complement(4876..5385)
                     /locus_tag="PWZ48_000222"
     CDS             complement(4876..5385)
                     /locus_tag="PWZ48_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259915.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(5560..7695)
                     /locus_tag="PWZ48_000223"
     CDS             complement(5560..7695)
                     /locus_tag="PWZ48_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2259916.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(7778..9433)
                     /locus_tag="PWZ48_000224"
     CDS             complement(7778..9433)
                     /locus_tag="PWZ48_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2259917.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(9435..9749)
                     /locus_tag="PWZ48_000225"
     CDS             complement(9435..9749)
                     /locus_tag="PWZ48_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2259918.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            9919..10179
                     /locus_tag="PWZ48_000226"
     CDS             9919..10179
                     /locus_tag="PWZ48_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HDL2259919.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            10193..10507
                     /locus_tag="PWZ48_000227"
     CDS             10193..10507
                     /locus_tag="PWZ48_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HDL2259920.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(11237..11791)
                     /locus_tag="PWZ48_000228"
     CDS             complement(11237..11791)
                     /locus_tag="PWZ48_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDL2259921.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(11917..13846)
                     /locus_tag="PWZ48_000229"
                     /pseudo
     CDS             complement(11917..13846)
                     /locus_tag="PWZ48_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
     gene            complement(14152..14868)
                     /locus_tag="PWZ48_000230"
     CDS             complement(14152..14868)
                     /locus_tag="PWZ48_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2259922.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(14869..16692)
                     /gene="glpO"
                     /locus_tag="PWZ48_000231"
     CDS             complement(14869..16692)
                     /gene="glpO"
                     /locus_tag="PWZ48_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HDL2259923.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(16706..18202)
                     /gene="glpK"
                     /locus_tag="PWZ48_000232"
     CDS             complement(16706..18202)
                     /gene="glpK"
                     /locus_tag="PWZ48_000232"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDL2259924.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(18338..19798)
                     /locus_tag="PWZ48_000233"
     CDS             complement(18338..19798)
                     /locus_tag="PWZ48_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2259925.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(19788..21083)
                     /locus_tag="PWZ48_000234"
     CDS             complement(19788..21083)
                     /locus_tag="PWZ48_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2259926.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(21415..22092)
                     /locus_tag="PWZ48_000235"
     CDS             complement(21415..22092)
                     /locus_tag="PWZ48_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HDL2259927.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(22122..23213)
                     /locus_tag="PWZ48_000236"
     CDS             complement(22122..23213)
                     /locus_tag="PWZ48_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HDL2259928.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(23314..23748)
                     /locus_tag="PWZ48_000237"
     CDS             complement(23314..23748)
                     /locus_tag="PWZ48_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2259929.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(24332..25789)
                     /gene="ascB"
                     /locus_tag="PWZ48_000238"
     CDS             complement(24332..25789)
                     /gene="ascB"
                     /locus_tag="PWZ48_000238"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2259930.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(25786..27681)
                     /locus_tag="PWZ48_000239"
     CDS             complement(25786..27681)
                     /locus_tag="PWZ48_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2259931.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPIILLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSSVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(27739..29514)
                     /locus_tag="PWZ48_000240"
     CDS             complement(27739..29514)
                     /locus_tag="PWZ48_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2259932.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARFPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(29564..31060)
                     /locus_tag="PWZ48_000241"
     CDS             complement(29564..31060)
                     /locus_tag="PWZ48_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2259933.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(31254..32876)
                     /locus_tag="PWZ48_000242"
     CDS             complement(31254..32876)
                     /locus_tag="PWZ48_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2259934.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESIKAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTIPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(33204..34517)
                     /locus_tag="PWZ48_000243"
     CDS             complement(33204..34517)
                     /locus_tag="PWZ48_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2259935.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(34798..34986)
                     /locus_tag="PWZ48_000244"
     CDS             complement(34798..34986)
                     /locus_tag="PWZ48_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259936.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKE"
     gene            complement(35006..35428)
                     /locus_tag="PWZ48_000245"
     CDS             complement(35006..35428)
                     /locus_tag="PWZ48_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HDL2259937.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(35573..36283)
                     /locus_tag="PWZ48_000246"
     CDS             complement(35573..36283)
                     /locus_tag="PWZ48_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2259938.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(36301..36798)
                     /locus_tag="PWZ48_000247"
     CDS             complement(36301..36798)
                     /locus_tag="PWZ48_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2259939.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPKHLADYLTQAYAPEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(36835..37287)
                     /locus_tag="PWZ48_000248"
     CDS             complement(36835..37287)
                     /locus_tag="PWZ48_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2259940.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(37422..38027)
                     /locus_tag="PWZ48_000249"
     CDS             complement(37422..38027)
                     /locus_tag="PWZ48_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2259941.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(38582..39217)
                     /locus_tag="PWZ48_000250"
     CDS             complement(38582..39217)
                     /locus_tag="PWZ48_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2259942.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKKSMTISNKEMA"
     gene            complement(39219..40088)
                     /locus_tag="PWZ48_000251"
     CDS             complement(39219..40088)
                     /locus_tag="PWZ48_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HDL2259943.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYISMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(40111..40518)
                     /locus_tag="PWZ48_000252"
     CDS             complement(40111..40518)
                     /locus_tag="PWZ48_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2259944.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDRHKLAELYEQVGLKFSELYENDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRSD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(40537..41709)
                     /locus_tag="PWZ48_000253"
     CDS             complement(40537..41709)
                     /locus_tag="PWZ48_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2259945.1"
                     /translation="MSVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAILWMATMTTVFGY
                     FAGLRGIVLILVGGFIGACFAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVA
                     KLFGEKGENKKSIEDIKLPTHFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGD
                     QNYIMFAFLQAIQFVVGVYVLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFP
                     YSPNAVILGFITTTIGTIIAMFTLPMFGLAMILSGMLTNFFAGGTAGIFGNAVGGRRG
                     AIIGGIAHGFFITLLPALLVTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(41736..42038)
                     /locus_tag="PWZ48_000254"
     CDS             complement(41736..42038)
                     /locus_tag="PWZ48_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2259946.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(42063..44149)
                     /locus_tag="PWZ48_000255"
                     /pseudo
     CDS             complement(42063..44149)
                     /locus_tag="PWZ48_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
     gene            complement(44638..45403)
                     /locus_tag="PWZ48_000256"
                     /pseudo
     CDS             complement(44638..45403)
                     /locus_tag="PWZ48_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
     gene            complement(45514..46383)
                     /gene="mdcB"
                     /locus_tag="PWZ48_000257"
                     /pseudo
     CDS             complement(45514..46383)
                     /gene="mdcB"
                     /locus_tag="PWZ48_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
     gene            complement(46408..47492)
                     /locus_tag="PWZ48_000258"
                     /pseudo
     CDS             complement(46408..47492)
                     /locus_tag="PWZ48_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
     gene            complement(47516..48109)
                     /locus_tag="PWZ48_000259"
     CDS             complement(47516..48109)
                     /locus_tag="PWZ48_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HDL2259947.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWATEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(48113..49789)
                     /gene="mdcD"
                     /locus_tag="PWZ48_000260"
     CDS             complement(48113..49789)
                     /gene="mdcD"
                     /locus_tag="PWZ48_000260"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HDL2259948.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDFIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEETIVISIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKFRQAGYPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(49779..50078)
                     /locus_tag="PWZ48_000261"
     CDS             complement(49779..50078)
                     /locus_tag="PWZ48_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HDL2259949.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(50180..51826)
                     /gene="mdcA"
                     /locus_tag="PWZ48_000262"
     CDS             complement(50180..51826)
                     /gene="mdcA"
                     /locus_tag="PWZ48_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HDL2259950.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVVADKADKDGNL
                     YTGFNTEETPTIVEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADEPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALVVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLREAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(51833..52744)
                     /locus_tag="PWZ48_000263"
     CDS             complement(51833..52744)
                     /locus_tag="PWZ48_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2259951.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLTFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEMVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            52864..53154
                     /locus_tag="PWZ48_000264"
     CDS             52864..53154
                     /locus_tag="PWZ48_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2259952.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLYTLEEGINYCLRSMRRSLLPSYCI"
     gene            53106..53720
                     /locus_tag="PWZ48_000265"
     CDS             53106..53720
                     /locus_tag="PWZ48_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2259953.1"
                     /translation="MSSVNEKIVTSVILYLEVASVSIPNLVRIFMEKHPDIQLTIMQH
                     HLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAIPKKHPLAKKEHLQLEDIQRTPIL
                     MLSKSNAFRHTLDAALEAKSIELTISSTTDDPATLRSIVKQGLAVSFFPKVSWSYDKN
                     DPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAKTMREFYLGK"
     gene            complement(53907..54269)
                     /locus_tag="PWZ48_000266"
     CDS             complement(53907..54269)
                     /locus_tag="PWZ48_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDL2259954.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            54615..56052
                     /locus_tag="PWZ48_000267"
                     /pseudo
     CDS             54615..56052
                     /locus_tag="PWZ48_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            complement(56059..58611)
                     /locus_tag="PWZ48_000268"
                     /pseudo
     CDS             complement(56059..58611)
                     /locus_tag="PWZ48_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(58725..60056)
                     /locus_tag="PWZ48_000269"
     CDS             complement(58725..60056)
                     /locus_tag="PWZ48_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2259955.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYSLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELAAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQMIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCTHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(60022..61500)
                     /locus_tag="PWZ48_000270"
     CDS             complement(60022..61500)
                     /locus_tag="PWZ48_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2259956.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPKKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(61805..63640)
                     /gene="tdc"
                     /locus_tag="PWZ48_000271"
     CDS             complement(61805..63640)
                     /gene="tdc"
                     /locus_tag="PWZ48_000271"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2259957.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTTHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHMTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            64109..65536
                     /locus_tag="PWZ48_000272"
     CDS             64109..65536
                     /locus_tag="PWZ48_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2259958.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQVSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRLTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERTDRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(65628..65993)
                     /locus_tag="PWZ48_000273"
     CDS             complement(65628..65993)
                     /locus_tag="PWZ48_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2259959.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQNDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(65990..66355)
                     /locus_tag="PWZ48_000274"
     CDS             complement(65990..66355)
                     /locus_tag="PWZ48_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HDL2259960.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            66793..66921
                     /locus_tag="PWZ48_000275"
     CDS             66793..66921
                     /locus_tag="PWZ48_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HDL2259961.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            66978..68672
                     /locus_tag="PWZ48_000276"
     CDS             66978..68672
                     /locus_tag="PWZ48_000276"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HDL2259962.1"
                     /translation="MRKSKSIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKSNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            68676..68795
                     /locus_tag="PWZ48_000277"
                     /pseudo
     CDS             68676..68795
                     /locus_tag="PWZ48_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            68799..68918
                     /locus_tag="PWZ48_000278"
                     /pseudo
     CDS             68799..68918
                     /locus_tag="PWZ48_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            69446..70579
                     /locus_tag="PWZ48_000279"
     CDS             69446..70579
                     /locus_tag="PWZ48_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDL2259963.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNVLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            70610..70900
                     /locus_tag="PWZ48_000280"
     CDS             70610..70900
                     /locus_tag="PWZ48_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HDL2259964.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKQ"
     gene            complement(70960..71419)
                     /locus_tag="PWZ48_000281"
                     /pseudo
     CDS             complement(70960..71419)
                     /locus_tag="PWZ48_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(71549..72802)
                     /locus_tag="PWZ48_000282"
     CDS             complement(71549..72802)
                     /locus_tag="PWZ48_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2259965.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(72831..73148)
                     /locus_tag="PWZ48_000283"
     CDS             complement(72831..73148)
                     /locus_tag="PWZ48_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2259966.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIIDLYRR
                     LNA"
     gene            complement(73174..73494)
                     /locus_tag="PWZ48_000284"
     CDS             complement(73174..73494)
                     /locus_tag="PWZ48_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2259967.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDIINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(73959..75374)
                     /locus_tag="PWZ48_000285"
     CDS             complement(73959..75374)
                     /locus_tag="PWZ48_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2259968.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREHEERYMDEKGIVQDDYRIEF
                     IGQHLRYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(75390..76706)
                     /locus_tag="PWZ48_000286"
     CDS             complement(75390..76706)
                     /locus_tag="PWZ48_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2259969.1"
                     /translation="MRGSGMSFMDKLAKVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISYLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(76856..77554)
                     /locus_tag="PWZ48_000287"
     CDS             complement(76856..77554)
                     /locus_tag="PWZ48_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDL2259970.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNIA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNVLAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(77843..78803)
                     /locus_tag="PWZ48_000288"
                     /pseudo
     CDS             complement(77843..78803)
                     /locus_tag="PWZ48_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
     gene            complement(78917..79750)
                     /gene="msrA"
                     /locus_tag="PWZ48_000289"
     CDS             complement(78917..79750)
                     /gene="msrA"
                     /locus_tag="PWZ48_000289"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2259971.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDTRENISVKEQ
                     LSKLEVTLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQDERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(79984..80727)
                     /gene="truA"
                     /locus_tag="PWZ48_000290"
     CDS             complement(79984..80727)
                     /gene="truA"
                     /locus_tag="PWZ48_000290"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2259972.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYRS"
     gene            complement(80918..81553)
                     /locus_tag="PWZ48_000291"
     CDS             complement(80918..81553)
                     /locus_tag="PWZ48_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HDL2259973.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIAWLSILHLKQ"
     gene            complement(81700..82941)
                     /gene="sstT"
                     /locus_tag="PWZ48_000292"
     CDS             complement(81700..82941)
                     /gene="sstT"
                     /locus_tag="PWZ48_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDL2259974.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            complement(83262..83939)
                     /locus_tag="PWZ48_000293"
     CDS             complement(83262..83939)
                     /locus_tag="PWZ48_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HDL2259975.1"
                     /translation="MGLAFLLAGCTNGNESKKESTKETAKATIATTTTTSTESKPTAA
                     STETADTSTSSESSKTTETTQTSTSSSSVEASSSESSEQVEQTLWNADKSAQLEAFMA
                     TWGQTMGQQYKSYTNQMSVDLYGLKVPQVILNGEWKMAIGGVPVSAEWSESGTGQADY
                     QITAVYSDAETEPYLKKNVYLFGFQQNQPKVLVTQQNQGNPDNYLYFNETANNELKNG
                     FNQIVYG"
     gene            complement(84190..84732)
                     /locus_tag="PWZ48_000294"
     CDS             complement(84190..84732)
                     /locus_tag="PWZ48_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2259976.1"
                     /translation="MNIVEEKFAQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLKQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(84737..84964)
                     /locus_tag="PWZ48_000295"
     CDS             complement(84737..84964)
                     /locus_tag="PWZ48_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2259977.1"
                     /translation="MKKMIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSTKVKEA"
     gene            complement(85043..86923)
                     /locus_tag="PWZ48_000296"
     CDS             complement(85043..86923)
                     /locus_tag="PWZ48_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2259978.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIIIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTARVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDNVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDQFSQYYTPTVLLIALLVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQRGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAMLKQAGAKHLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLH
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSSFGSLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLIGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            87209..87544
                     /locus_tag="PWZ48_000297"
     CDS             87209..87544
                     /locus_tag="PWZ48_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2259979.1"
                     /translation="MGIEAVDKYLYLLAGNKIQKSLMDFIQELECTFHKKFTHSILLK
                     LLIHTACLIERTLINGHELKIISEDDTRPSHETIFHVKKAFKNIETEFGITVSYDECF
                     FIYDIIASK"
BASE COUNT        25648 a        18555 c        14798 g        28682 t
ORIGIN      
        1 tttattgaac ttaaggttga ttgatcacat ctaaaagcgt tgaaaataaa atggcactag
       61 aagcagctcc tgggtcgata tgtccgattg cacgctctcc tagataggaa gctcgtcctt
      121 ttttagctac caagactttg gtgtgttctt ttgcctggtc aatgatttct tttgttaaat
      181 tccctgcctt caatgcctct gtgactggca gccaaacatc taacatcgtt ttatcgtcag
      241 cttctgcctt tcctcggctt tgaatacccg ctgttccctg ctcgatcact tcgcctaatt
      301 tttcttgaga atcaatagaa tcgacctctt ttgtcgcttt catcatgttc atgaatgcag
      361 atccgtataa cggaccagaa gccccaccga ctttcgatat catcgccata gagaatactt
      421 tgaatgtatc gctgattgtt tcaggatgat cggcttgaaa agcttcttca taagctgaaa
      481 cccctcttcc catgttattt ccatggtctc catcgccaat cggggtatct agatcactga
      541 gatattgttt gttctgattg attgcctctg caaattgcgt gagccattct tggatttctt
      601 gaacggttag tttcatatta tcaccctttt atcttgagat tttttaccat gaaatcgtct
      661 gtacattgct ttctaaagaa tctttccatt gtgtctccgt caagtcgatc agtgttagag
      721 aaagaccttg catatctagt gaggtcataa aattaccgac ttttcggaaa actacattga
      781 cgcctctttc ttccaacaga gctaaaacat cattcatgaa cacgaactgt tccatcaaag
      841 gcgtcccgcc cattccgttg actagtacac ctgcttcttt tggtaaattc gagtaatcat
      901 ctaaaatctt agtaacaagt tcctttgcca gtactttaga aggctgcatc ttttctcgac
      961 gatagcctgg ctcaccgtgg ataccaacac catactcgat ctcatcttct cctagttcga
     1021 atcctggttt ccctacttct ggtacagttg ctgctcgaag cgctagaccg atcgtcttag
     1081 ttgcctgtac gaccttttct cccagttctt tcaactcagc tagggaagca ccgtttcttg
     1141 ctgcatcgcc cacgattttg tgtacaagga cagtacctgc aactccgcgt tttccagcag
     1201 tatacgtact attttcaacg gcgatatcat catctacgat cacactttct acttcaatat
     1261 cgtccatagc agctaattcc tttgccatat cgaagttcaa tgcatcccct gtataattct
     1321 taacgatcag caaaactcct tttccactat cggcttcctt aatagccgtc tgaatctgat
     1381 ctggagttgg agaagtaaac acatcaccaa gcaccgctgc acttaacatc ccttccccta
     1441 caaacccggc atgggatggt tcatgtccgc ttcctccacc agaaaccaaa ccaacttgtt
     1501 ccgctttctg attcttggct acaacacggg aagattctac acgatgaaca agttctggat
     1561 agctttttac taatccttcc agcatttctt cgacaatatt gcttggatca ttcatgattt
     1621 ttttcaattt attcccatcc tttctttccg gcattttgtg cttctgttaa gatttgctga
     1681 gtatcatctg ttataccggc agtgatggct gctgcaaaag ctccttccac taaaggcgca
     1741 tccaccagat gataacgttg ctgctgctct tcttccaaca tgtcttttgc cagttccgag
     1801 ttcaataccg cgctaccgat atccgcaaaa acaaaataac tagctgcatc gcttgattca
     1861 ttgactgctt ctacgatttt catcgggtct gatccgatct cgccatttat tccgccgaga
     1921 gaaaaaattt gcacctcttc ggatttttgc atctgctcaa tcatttcttt aataccatct
     1981 gttacttttt gactatgaga aataagcaaa atactttttt tcacaagaat cttcctttct
     2041 tttacatagt tgctaacgat tggtttcgct ttcattaatg aacaattcaa gtatataata
     2101 aatttctaat tttgggaaaa tatgtgtagt gcttcttgaa gtttgttata ctttaattaa
     2161 aagagaggag tgatcgaatg attacaacaa caacgaacag tgtagaggga aaagccgtag
     2221 tagcttataa agggatcgtt tttggtgagg tcattactgg gatccatgca tttaaagact
     2281 tggaggcggg gttgcgaaat atctttggcg gccgctcaaa aagctatgaa aacgaactaa
     2341 tgggtgcaag agaagaagca cttgccgaaa tggccgaacg tgcaaaagca ttaggagctg
     2401 atgcgatcat tggcgtaaaa atggactatg aagtattagg cagcgacaat ggcatgctga
     2461 tggtcacatg tagtggaaca gctgtctcca cgagataagt aggggacatc tgttttaata
     2521 actaaagaga gatgggacat gtagtggcac tcaagaatta gatttttcag tctaacttcg
     2581 aggtactcta ccttcccatc ctctcttttt tatttctttt tgcggatgat tccgtaagct
     2641 gctgcgacta acacacccgc ccctgctaag gaataactga cagaagctgt tttttcattt
     2701 gtttttggca aagtctgact tgttgatttg gatggctctg tcgtttgctg aactgtttct
     2761 gtcgtgcttc cagaggtcac tgatttttct cctgccgaat gcaaaatatc ttttattcgt
     2821 tggaaactag ctgctaagtc agcttttttt gcttcaaaat cattagcaac tgtttgtgct
     2881 tcgctgatct ttccgtggat tgcttgactg gccgctgtga tatccgcttg agtttgactt
     2941 agctttccag aagctgtttg agcaacctga tttaattgct gattgaattc ttttgcttgt
     3001 tggatcttcc cgtcaagatc tgtttggaca gcttccgctt ttgctttcgc ttcggcaagc
     3061 tttttctcaa gttgtgcacg gtcatttccc actttatttt taagatcagc taaatcttga
     3121 tcgattgctg cttggtcttc tttcaatttg ttttttgcag cttctaatgc ttcttcgagt
     3181 tcttttcttt tattttccaa gtcttgcaaa tgatcgattt ggtcagaaga ggtagaacta
     3241 tttattgatg tatcttctga agatgaaatc gtttctcctg aattatctgt cggtacattg
     3301 gaagtcattt ctgtttccga ggcaccgttt tcagcaccag aagagcttat ggttgttgaa
     3361 ggttctgttt gagcaggttc agaagtttcc gcaaaagctg tctgtcccaa taagaaaata
     3421 ctggaaacag caattggcca gattaacggc ttcattcttt ttttcatcac ttttcccatc
     3481 cttactactt tttaactaat ttcttattat aagcaaaaat gtttggaaga accataaaca
     3541 caacaggctg tttgtctaaa aaacggtgca tcttttattt ttttgtacgt tccgggcttt
     3601 tccatgtccc gcttccataa cctaaaatcg ttttgactgc tggaatgaaa gtcgtcacga
     3661 tcgttatcgc gttcactatc cagaacaaaa gcatatatag tggtgcgaaa agaaggtatt
     3721 tcaattttct tcgtcgatca tctacaatca gcgaagctag caactggaaa aatcctgcga
     3781 ccatttcaaa gcagatgaac acaaaactga atgtaaaagt aatataaatc tgttcgtagt
     3841 ttccttgata tacataatgc ccgattaatc cagcaaaaag aactacggaa atacaaaaga
     3901 atattgacca gacaatactc aatgtttgat caatgaagat gatcgttctt ccaatatgtt
     3961 taaaaggatg gagcatcact tttttgaaat tcgtcagcca gacttctgtt ccaccttttg
     4021 cccaacgttt tctgtgtcga tatagcatct tcaacgtcac aggtacttcc atgaaaaaga
     4081 taatctcagg agcaaaaaca gataaccagt catttaactg atgatcccaa gcaatactga
     4141 tgtcttctgt cgctcgatct tgtctgaatc cgcccacatc gatcaaggct tcttttcgat
     4201 acatcgtatt tgctccactg tacgcataaa gaccaccgaa aacagctgac tgtgttcgtt
     4261 taatgatgcc cacgatactt gagaattcaa ccgtttgaga ttttgcaatc aatttggtac
     4321 gattttggac atccatattt gcagtgaccg cagcaatatg tcttgcacca gggcgaatga
     4381 aataattcac atacctaatc agtgcatctg gctccggtac cgtgtctgca tcattgctta
     4441 aaatcaattt cccttttgca aaggcaagtc cgatattaaa cgcgtgggct tttcctttat
     4501 ttttttcgat ccgaaccaca cgaagattag cgtatttttt catcagtcta gctaatattt
     4561 ctggtgtttg gtccgttgaa ccatcgtctg tcactaatac ctcatagttg tgataattca
     4621 atttcgtcat caaatactcg attgtatatt cgatcacaat ttcttcatta tgggctggca
     4681 ccatgatcgt gatgaacggt tcgacactca agggaacatc tacccattct ttttgcttat
     4741 gtttgtataa aaacacataa caccagacac caataaacca tacaaatccg cctaaaatcg
     4801 gataagaaac gagaaaaaaa tacataatag acaaacaagt atctaagaat aaattacctg
     4861 tatgggtgat tgagatcatt tcagttccac cccattttct ttatacaaat ctgcaatgaa
     4921 tgtcgtatct agatttttct cttcggagac agaataaaaa cggaccgttt ctctctcttc
     4981 ttttgttcca aaacgttccg tatacacttc ttcaagtaat tcttgtcttt ttttcaattt
     5041 ttcatcttca tattcaaaag atttatttac ttttttgtat agatttcgat tgttccacct
     5101 agtcaataca aatgaggtaa tcaggaaact gaaaaaaatc aatacaaaaa agccgattaa
     5161 gaaatacagc attttctttt ctgataaaaa agcgtacaag gaaaaatcct tggctctttc
     5221 tggtctcgtt aatgggaaaa agacccatat aaaaggaaga acaacgatca tccatcctaa
     5281 aagagccact aacgtttgcc aaatctttaa ccagtaatta ccttttgaaa aatacttatc
     5341 tttcaccaaa attgatgcaa cttcttctct tgttactttt ttcatatcct cacctcacgt
     5401 ttttttgtta cgacgtagga gtttaccata catttcattt cttttcacat aaagtgttcc
     5461 taaatttttc ctaagaaaaa gaacaaaaca tttctcatca gcaaatgaaa aatattttgt
     5521 tctttttatc agttgttctt tttatttttt atctcttaat tatgttgctc tttccaatac
     5581 gacaatgctt ttcattcctc tagatatcgc cgtgtacatt tgtctgcgtc ttctttgctc
     5641 atctccatcg ttttcaaatg gatataagat tacatgatca aattccagtc cttttgctag
     5701 actcaaagga atgatttggc agttttctaa tccatatgaa ttttttttaa tcgattcttg
     5761 tagcgtttgg gtttcctctt ctgtttgggt gataactgcc gctttttctc cttttagaga
     5821 aaccaaaatc tcagctagta ttttcaacaa ttcttcagag gatcgaaaag agcggtattc
     5881 gggttctttt ccttgcggtc ggatcgatac gatgtcgatc ttctcttgat ccacagcata
     5941 ggttttgaaa agctctgtga ttgctccgct agaacggtaa ctatttctaa gatcataccg
     6001 atggatcggc agatggtagt cgtcgaaaca acgcataata tcagcaaaag tagtggaaga
     6061 attaaagatt gcctgattct cgtcacctac taaagtaaag tgagtttttg gaaataattc
     6121 gatcaataga cctaattgtg ccagggtata atcttgtact tcatcaatca atagatagcg
     6181 aaatgctggt acatttacct tttcaacaag taaatgacga atagctaata taatgactgc
     6241 ttcatcaaca gtacgtgtgg gttgccaagc ataagctgag ccatatcgtt tttcataaat
     6301 tctttcaaac aactgatgct catccaccca tgccatttct tcgatttgtc tggttatttt
     6361 tcgatatttt ttttgtaaaa gtttccttgc ataagctaca atactttgtt ccgaatcatc
     6421 agaaatcaat ttaccgaata attccaactg cctatcttct gtcaacgaga gtacctgatt
     6481 ctgcacagca ggactttttg cttgtttcaa aagatgattc ttccattcgc tcatcagtgc
     6541 ctttttcgtt gcttgaagcc tatcgatcaa tttaggatga gcaggaactt tctcatatat
     6601 tttttcgatc gtttttttag aaaggactgt ttttccattt ttagtaagtc cacggataaa
     6661 attcggatga tctaaaaaca aaggatctga ttgttttaga ttatcgataa actttttgct
     6721 acgcaaacgt tctgtctgct ccgatacatt ttctcccgtg atacgtttga aataagcctc
     6781 ttcaccttct atttcctctg ctgaaagctg tgaaaccagc tgtatgatgg ttagattcag
     6841 tggatttctt tcacctaatg atggtaggac attcgaaata tagtcgataa accgatgatt
     6901 aggtgataga atcagcaagt catcggatgt catcgtatct cgatataaat agagcagata
     6961 cgcaatacgc tgcatgatag cagaggtttt accacttcct gctacgccat ttaccaagat
     7021 attttgactt ttcacatccc gaatgattgt gttttgttcc gattggatcg tagcagtgat
     7081 agccttcatt tcattggtct catcttggcc taagacttct aataatacgt catcttgaat
     7141 agctacagac gtatcaaaaa acttgaccaa acgatctctt tccaaaagaa attgtcgtct
     7201 ttctttaata accgtatcga ttttattttg acgaaccgta taagaagatg gaccaatcat
     7261 gttattgtaa aaaagttcgg caattggtga acgccaatca taaacaagat tttcatctgc
     7321 atcatctgtg aaatttgctg taccgatata aaaggattct tttggctcat tatccagaaa
     7381 gtctacaacg acttttccaa agtagggact tttcaataaa cgttcgatct ttgctagctg
     7441 attttctgcg tttttctgtt tgatattcat ctgatcgatt tctctatttt tcatttcaat
     7501 catcgaaaaa gtatccaggt tatctaagta actgtcaaaa ttcaatcgaa gctcgccagc
     7561 catttctttc aacgactggt ttccttctat ttttgccgta tccatcgatt cccgcagttc
     7621 gtttttttta tcaattagct tttgatacac gttcttcaga tatgcttctt ctaactgttt
     7681 ttgtgtgttg accatacaca ttctcctctt tttttaaagt ggtggaaccg attagtttac
     7741 ctcttttaag agtcgaatgc aagcttttta acgagtatca tttgttagga tatatcagtt
     7801 cttcaggtcg gacagtttga tataaagaaa atgcaccatc aaggtttttt acgtggtagc
     7861 cagactgctt caagattctt tccgctagat agctacgtaa tccgctatga cagctgacga
     7921 tatattcttg ttggctgtct aattcattta atcggtcccg aagttcattc aaagggatat
     7981 gtttggcttc cttgaatttg cccttttcca attcttctgc tgttcgcaca tctaataaaa
     8041 tttttccttt tgctagttct gtcggcaatt catgccattg tacggtttca ctaagccctt
     8101 ctgcaatgtt cattgctgca tatcctagca tgtttactgg atctttggct gagccaaatg
     8161 gcggtgcata cgtcaattct aattccggta aatcgaagat cgtcaaatgc cctttgattg
     8221 ctgttgctaa aatatcgatc cgtttatcca caccttttgc cccaacacct tgtgcaccat
     8281 aaatctcgcc agtctctgga tggaagatca acttcagtaa aatatctgtt gcttctggat
     8341 aatagcttgc atgatctttt cctgatacat gaatgacttc aaaagacagt tcgttttctt
     8401 tggctgttct ttcacttaaa ccggtggatg cggcagtcat tccaaacgtt cgtataattg
     8461 ctgttccaat accgccttga tttcttcgag aaattccgga aatcgtgtcc gcaacttgtc
     8521 taccttgacg attggctggt gaagcaagag aaataagtgc atcttttcca gtgatttgct
     8581 gttttacaac gatagcatcc cccaccgcat aaatatcagg aatattcgtt tgataacgtt
     8641 cgtctactag gatacccccg cgtatgccta attcgattcc agcggtcact gctagctggt
     8701 tatcaggacg aacaccaatg gataaaataa ctaaatcagc agggatggta cgcccatcat
     8761 ccagtcgcaa tcgtttcccg tggtcttcaa atccagcgac agcatgagaa gtaatcactt
     8821 ctactccttg atgagacaat tctttttcta taaaagctgc catttcttca tccagtgttg
     8881 gaagaatatg tggtgcttgt tcgatcacca tgacttctaa acctcttctt tttaggtttt
     8941 ctgccatttc caatccgatg aatcctgcac caacgatcac ggcacgcttt ggctgttttt
     9001 ctaatgcatg catcacttta tcgatatctg gtacatttct aatagaaaaa acattgtcgg
     9061 cttctgttat ccctggaatc gatggaacaa ctggttttgc accaggagat aaaaccaatg
     9121 catcgtactg ttcaatgtgt ttttgaccct catgtacgac ttctaccgtt ttgttttcgg
     9181 gaaagatggc tgtcacttca tgatttggac gcacatctaa acaaaaacga gcggataatg
     9241 attctggcgt ttggacaaga agattttccc gttcactgat ttctcccgaa agataataag
     9301 gcaagccgca atttgcaaaa gaaacatacg gtcctttttc aaaaacaacg atttctgcat
     9361 cttccattaa tcggcgaagc cgtgtagcag cagacattcc acctgctaca cctccgacaa
     9421 taacgatttt catcttattt ccctcctact gttcgatcaa accattgatt catgccacca
     9481 cgtacattca ctacatcata accagatttc tttaattctt tagctgcttg tttactcctc
     9541 atccctgatt gacaaatcac atagacggtc ttttctttgt ctccatcata tctgtttata
     9601 ctttgtaatg gaacattttt agctcctttt atatgcccgc cccggtattc tgcaggtgtt
     9661 ctaacatcaa gtaacgctgg attttccaaa agcttttcct ttaattctgg gactgttatc
     9721 gattttattg attgaaatag caaattcatt ttctgcacct catttacatt tttataaata
     9781 cctcatgggg tatattaaac aggagcttta ttaatatgtc aaccagaatg actcttcatt
     9841 tttttcacta ctttttatat ttgcataaaa taccgataag gggtattatc taattataaa
     9901 aatagaggag aatgtgttat ggagattgat aataaaaata tcctgaaccg tttgaaacgt
     9961 acagagggac aaattcgtgg tatccaaaaa atgatcgaag acgaaaaaga atgtatggat
    10021 gtcatcactc aattaagcgc tgttcgttct agtattgatc gcgtaatggg catgatcgtt
    10081 gcagataatc tgaaaaattg ctttgagaaa cctgatagta atccagaaga acaagcggct
    10141 aaactagaac aagcgatcaa gatgattatt aaaaagtaaa ggaatgattt caatgaaagt
    10201 cgttttccat attgatgaat tagaaaaatg gtcagaaaca gggaaaaacg taaaaaacct
    10261 gataaaagct tctccagaaa cgacgattgt cgtatctgta aatggcatcg cgattaccgg
    10321 ttatctagac tctgccaatg cagaattctt agatttgcaa ggagtcgttt ttcacgcttg
    10381 cgccaatgct atgcgtgcta atcatatttc agaaagttct ttacctgaac aagtaatcgt
    10441 tgtaccagca ggtgtccttg atttagttga actgcaatcc caaggttatg cttatatcaa
    10501 gccttagtca gtagaggttg cgacagaagt gatcagcttc aagaaatttc ggcttatttt
    10561 tcgaagaagc tacttctgga aaaccgtgaa tcaaaggttg tgaaaacgct gtcctgcatc
    10621 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcgtttgg ttgggcttat
    10681 taccgcagat gcaagctttt gaacaccgtt taaggaagag gttgtgaaat cagcggtttg
    10741 acctaagtat tagagaaaaa aacgggaaaa tagctcctca tattttgccg ttttttgagc
    10801 taataccgaa ggtcagctga ttgaacaccg tttaaggaag agattgtgaa aaagtcgctt
    10861 agtttcgagt attaagaaaa attttgtgaa aatggatttc tacatttttg cataaatttg
    10921 gcttaatacc gagaaactgg cttttgaata ccgtttaagg aagaggttgt aaaatcagcg
    10981 gtttgacctg agtattagag aaacaaacgg aaaaatagct gctcatattt tgccgttttt
    11041 tgagctaata ccgaaggtca gctgattgaa caccgtttac caagaggttg tgagcctacc
    11101 atttaactcc gaatcacaag aaataatttc aaaaacaact tctcctgttt ttggttcggt
    11161 tggcttatta tcgagaagta gctactggga gtctatttat caaaaaaagg aatgcgacat
    11221 aggtcacacc ccttttttat tttctttgaa acatctttcg gtactcgaca gcatacaaga
    11281 caaaagattg taaaagaccg taagtgatga cggcaaaaat gactctcacc cacagcgttg
    11341 gtaattctgg gaaaagcagc aaaaggatca tgccaacata gatgacagca gtcgctactt
    11401 tgccatacca cttcgctcca cttaatttga tatttttaga tagcccgatt aatccagcta
    11461 caaacataaa gccttctttt atcaagaata gaccaaacaa cagccaaagc cacggtcgat
    11521 accatgccat agacaaaatc agaaaaagtt gtgtcaattt atctgcaaac ggatcaagca
    11581 ccttccctaa gtctgttacc tgattatatc tccgagcaat gaccccgtct agataatcag
    11641 acaaacctga gacaacaatg accgctcctg ctatataaaa tgactgtctc agcacaaaat
    11701 aaagataaat cggcagcaaa aaaatacgga atatcgataa aagattcgga atcgtttgta
    11761 tttctttacg ccagtctgtg cttaatttca ttttatcgcc ctacctcctc agcactcttt
    11821 tctttttatt ttagcttaca cggggaataa aaccaatgat ttcactgttt ttatttttct
    11881 ttacagacac tgatattatg agctaatttg agataatcac ttgctgtttt catcgtttac
    11941 tcttctttct tcctgtcgtt tgcgcatttc ttttactagt actttatgag ctttttttga
    12001 tcctcgctct aggtcacgct gccgtttacg tgttttataa tcgataaaca gactgtctaa
    12061 atcgtaacct gtcgggtaca gatcttctgc tgaacgcacc agacgaacac gctgacgagg
    12121 cacgtggatt atttttttgt ctaaatatac atcaatcgta tcttcaccta tatcttcata
    12181 aactaacgct tctttttggt attcattgac aaatatccga tcaccttttg caaagagctg
    12241 acgttctttt ttattctcct tcctatttgt aactgctgtc ttaggaagaa attctttttt
    12301 ctgtgtccga taggcttttt ccgccaagta acgctctgct tgatggatca atttcatcga
    12361 catatccatt ttatgagcaa tccacaaagc ttggctttct cctgtttctt ccaacccgca
    12421 actgatattt aggacgaagt gcttcccgat cgaaagccat agcagccggc acaaaatctt
    12481 catgatctcc ggcgaatttc ttgatttctc cataatgggt cgtggcaatg atcaatgccc
    12541 cttgctcata catagattcc atgattgcga tcgctagtgc agccccttca tttggctctg
    12601 ttccactgcc aatctcatct aaaagtacca acgtattcct ttttatcttt ttcaaaatag
    12661 cagcgatgtt ttgcatatga ccagaaaatg tactcaacgc attttctaaa ttttggtggt
    12721 caccgacatc gacgaaaatc tcatcaaaga cagcaagctc tgtcccttct tttgccggaa
    12781 cttgcatacc gaacatcgcc atcaaagtca ataaaccgac tgtcttcaac acgactgttt
    12841 tgcctccggc atttgctccg gtgataatca agcctcgata gtctttaccg atttcaacat
    12901 ccaatggtac cgcatgctca ggtaaaaatg gatgtctgcc ttgcttgata atgatatgtt
    12961 ccgatttatt gattctaggt gtgcttccat tgatttctcg gctaaatttt gcccgagcaa
    13021 agatgatatc taacaccgtg atcgtttcga tcaataaatc gatcacttct tcgttttctg
    13081 cgatagcacc agtcaatgcc gccaggactt gatattcttc actgatttct tcagctttta
    13141 acaactgata gtgttcattc aatttagaaa tcacagttgg ttcaataaaa accgtcgttc
    13201 ttttatttga ttcatcgata atcgttccat ctattttatt tttatagctg gctttgatag
    13261 gaatcgtata gtattcgcct ttttgaacaa tcattgcttc ttggatcatt tctttgtttt
    13321 tcggatgacg caagaatttc agtaattttg actggatttc tttttcgatt agttgtaatt
    13381 gtttgcgtac tttacgcaaa ttacgtgacg cgtcgtcact gactttatta ttttttattt
    13441 tctggtcaat caactcttct acatttatca aatcaggaag atgtttgcta taagcaaaca
    13501 acaagggtgt ctggtattga ttcttatcaa aaaactttgt gatcatccga ctgcttctaa
    13561 gaaaatccgc atattcgatc aaatcagttg gctgcagaat caaccctttt ttgacttgtt
    13621 ctgtcagtgc atcgatcctc ggcaacccca taaatggaac atgctgactg ctttctaaga
    13681 tcaagcgtgc ttctcttgtt tcttcttgac gatccaagac agtttgtagg tttgtcgaga
    13741 ccgtcatatc gctgattcgt tttttgctat agtttccaat tgctcttgct tggatttcct
    13801 ttttgatccg atcaaattgt aattgactaa tcatttcgtt gtccatattc tctccttttc
    13861 ttgaagagat acctgtccca aaataaataa gggcctagag ataaataaaa ggatagaaca
    13921 cagcttaaaa aagtcatgtc ctatccttat tagaatacaa agatattgtt ttcctgacag
    13981 gtaccgtttc ataagcacac caaataaact ttgtttattg gctattcgac ttactgatta
    14041 gataccagct acactcacaa aacatacccc attcttctag gtcttattta ttttccactt
    14101 aactatagca agacacattt tggttgtcaa actttagtat gttttataga tttacaccat
    14161 taatgaatag acacctaccg cgataattgc tccgatcatt ggacctgcga ttggtaccca
    14221 tgaatatgcc caatctgaag ttcctttatt agctattggc aataccgcat gtgctaatcg
    14281 aggaccaaag tcccgagcag ggttgattgc atatcctgtt ggccctccta atgaaagacc
    14341 gatggctaag atcaatccac ctacagcgaa tacgttcgtt ccaggtgcaa attcgttttg
    14401 tccaaaagct agcaacccaa gaactaatac aaatgtacca attaattctg tgatcacatt
    14461 tgctggataa ttccgtatag caggacctgt tgcaaacgtt cctaaaatcg cgctttcatc
    14521 tttcgttgca ttccaatgtg gcaaataaga aagccaaaca agaatggccc ctgcaaaagc
    14581 tcctaaaact tgcgcaatga tgaaaggaac gacaagattc caactaattg cccctgtcat
    14641 cgccatagct agagagactg caggattcaa atgagctggg cccatgaatc cagaaatata
    14701 gacagccatt gttacagcga gtccccaacc aaatgcgatg acaatccagc cagaagcctg
    14761 tgctttactt ttgtttaaat tcacagccgc acacacacca tctccaagca aaactaggat
    14821 cagtgtccca ataaactccc ccatgatttg agtcatatca ctgttcattt atttctcccc
    14881 ctttagttcc cgcagatctg attctgcaat caatgcttct aaacgtttag tctgttgcgc
    14941 cttctcctct tctgtccatt caaaatatcc agcaattgca tcgatcacag gttgcttgat
    15001 ctcatcaagc tgatctcgtt caaacaacag atgatttgta cgacgaatga gataatcgcc
    15061 tggggccaaa accatttctt cttctaaacc ataacgtaac cgagctgact cagcgagact
    15121 caaaccagga taaggtgcca tttcttttgc taattcaaag atacgtcggg cattagtccc
    15181 gtaaaaatca gcgatataag tagcatcttc ttcctctaaa ccggctgcta ctccttcttt
    15241 tgctaattct gtcactgttt cttctaattt agttggatcg aagtttccac ctgaaatctg
    15301 atatttttta gagtcgattt ctttcgtctc tattccgtat tcttctttta ataattggcg
    15361 tatcaatcgc aaagctcctt ctgccatctt acggtaatcg gtgattttcc caccagataa
    15421 agtcagcaaa ccatctggtt ctctttctaa agaactacct ctggaaatcg tagaaggtgc
    15481 tttttcatca cggctgtttt ccaaatggtt caacacgtct tctacttcag cacgagaaac
    15541 tttgttttcc ttatattcac ttactgtatc gacaacttta ttgaagctct tgtctgaaat
    15601 cgatccatta tctccaccat tgtaatcaga accagaatta ccaatcaaca gtggacgaag
    15661 tcctgcccag cttgcttcga tatcattcaa tgtgatattt gcttctggat agcgatgatt
    15721 gatcacatcc aatagataat ctacatcttc ttgtgtgact ttcggatccg taaaatctcc
    15781 ttggtaatcc gtatctgtcg taccaaagta agtcttgttt tctcttggaa tagcaaaaac
    15841 catccgccca tcttgctttc ctgtatcgaa gtatgtgggt tgcggtacag gcagtttttt
    15901 cgcatctaca actaaatgga tccctttagt tggacgcatt ttaggagaga ctggacgcgt
    15961 aaaattcaag tttcgtactt tatctaccca aggaccactc gtattgatca ctaattttgc
    16021 tttgatttcg atcacttcat ctgtcagcag gtcacgagct ttgacgccaa cgatttgatc
    16081 gccatcatat aaaaagccaa ccgctttcat tttacttact agataagccc catcttcggc
    16141 agcctttttg atattatcaa tcactaaacg ggcatcattg ttgcggaaat ctagatagac
    16201 acccgcgcct tttagccctt cttttttcaa aaacggttct ctttccaata cttcttctgg
    16261 tgtcagcgta taattctcat atttggttcc tgtcacattt gccagtttgt cataaaggtc
    16321 cattgctact ttgaccgaga acatattgaa ggttgttgct ccttcgccct catagattgg
    16381 caaaagcatt ggatctggtt ttgggatgtg tggggcaata ctttgtacga ctgcacgttc
    16441 accaactgtg tcagctacta cttccacatc aaatgtcttc agataacgaa taccgccatg
    16501 aacaagtttg gtcgagcgag aggaagtccc ttctgcaaaa tcctgcattt cgatcaatcc
    16561 tgttttgatt cctgatgctg ctgactggac tgccacaccg gcaccagtga ttccgccacc
    16621 gatgatcaac agatccaatt cttcttgctg cattttttga atgctatctt gtcttgtctt
    16681 gtttgaaaac atatggattt cctccttatt caaatgcttg ggtagctttt actgcttttt
    16741 tccaaccgtt gtaaagtttt tctcggcgct cttcctccat ttgaacttca aatagcttgc
    16801 cagcttcata aaattcgcgg atctcgtctg tatctttcca ataaccgaca gctaatcctg
    16861 ctaaaaatgc tgcccctagc gccgttgttt ccaagttttc tgctcgttgg atcggtacat
    16921 tcaagatatc tgtttggaat tgcatcagat attcattgtt tgctgcaccg ccatctactt
    16981 tcaatacagg aatcttgatt ccagtgtcat cttgcatcgt atcgatgata tctctcactt
    17041 gataagcaat cgactgtaaa gtagctttaa tgatatcttc ttttgtcgta ccacgtgtca
    17101 agccaaacat agcccctcgt gctgcctgat cccaataagg agcacctagc ccaacgaatg
    17161 caggaacaac ataaacctca tcttcgcttg tagagcgttt cgctgcgtct tctgaatcgc
    17221 tagctttttg caacatttga agtccatctc tcagccattg aatcgaagaa ccagccacaa
    17281 aaatacttcc ttctaaagca tagtaaacct ttccatttat gccgtaacca attgtcgtca
    17341 ataaattatt tttggataat tgcggttttt caccggtatt catgacaata aatgaacctg
    17401 taccataagt attcttgacc atgcctggtt cgaatgccat ttgtccgaat aatgcggctt
    17461 gctggtctcc tgccatccct gaaattggtg cttcgcttcc ataaaaatga taaccttgcg
    17521 tataaccata aacttctgag ttagaagtga cttttggtaa catcactcga ggaatattta
    17581 gtaaatctaa aatctcctga tcccaatcta gctgatggat attgaacaac atcgtccgac
    17641 tggcattcga gtaatcagta acatgtgctt gtccatcggt cagtttccat actaaccaag
    17701 aatcgatcgt tccaaacatc aattcgcctt tttcagctcg ttcttgcgcg ccttctacat
    17761 gatcaagaat ccagcgtatc ttcgttgctg agaaataggc atcgatcact aaccctgttt
    17821 tttcgtggaa aaattcttga tagccttcat tttttaagtt atctgcaatt ccagctgact
    17881 gtctggattg ccaaacaact gcatgataga ttggacgtcc tgtatctttt tcccatatta
    17941 ctgttgtttc acgctggttc gttatgccaa tcccagcaat ctgatttggt ttgatccctg
    18001 attcgatgaa cgctccggca ataactgact gtacagaatt ccagatttca tttgcatcat
    18061 gttccaccca actttcttgc gggaaatatt gcgtgaactc tttttgagaa ctgccgatgt
    18121 gtcttccttt cttgtcaaag ataattgctc gtgagctagt tgtaccttgg tcaatcgcca
    18181 tgatatacgt tgattctgtc attgtgattc cctccattat atgcaaacgc tttctttaac
    18241 tactcttatc atactactgt tttaaaaaaa gaaactgtca aaattttatt ctttttttct
    18301 ttttttgaca gttttttatt atgaagctat tttttaatca attcttttct tttttcagcg
    18361 tctctattct tcttttcaat cgaataatgt cgtaaggtga cgcaaattct gacagataat
    18421 attcacactg ttttttgttt ctctggcaaa atgtgatcac caaatcatat tctttacctt
    18481 cttcataact tccaatctca ataccgccga ttcccctcag ttctctgatc agatattgtt
    18541 gaaaagcaat acgataaatc ggcagacttt ctaaatcgat tccaatcact actggttttg
    18601 caatataaaa atcgatcatc aaaagaacat tggcatactg gatcatcagg taactaagct
    18661 cactatcttc agctcttttc ctcggcaatt gctgcaccgc tgtttcctgt aatttcgcca
    18721 gtaactgtgt taaggaatgc ccgagcattt tctgctgctg ttccaaaagc cgatcacgct
    18781 catagacttc gatctttcca acaaagaaaa accattcatt gttgatctgg gaaatttggt
    18841 agcctactgc cttttcttct ttgattttca aacgacgagg acgataatgc cataacatcg
    18901 tttcccgaat gtaagtatct aaaacagcgg ttggtaactt tttgctccgt gtcaaatcat
    18961 actgataaaa tatttcttcg tctactatcg aaaaagaaac aaaaaagctg taaaacatca
    19021 ttgtttcgta tacattcagt tctgctgctg ttcgactaag atgcatcacg atgattggat
    19081 cgattgcttg ataaagccgg tccgactgat agagtaattt cttttctttc aaagttgcgt
    19141 acgtactttt ttcattcaat agtctttttt tcgtaatccc gagccagcaa gcaatctgtg
    19201 cttctgctga tgccgtaaac gttgtttcta atacacggtc caatcctaga atgaacggtc
    19261 ttttttctgg tgtattttgc aaaaaaagcg gtcgctgatc ttccgggata tattggaata
    19321 gttcataata aaaatatcgt atctgtagct cttctccgta taattgaccg tttttgattt
    19381 gtaatccaaa ttcctctaag agctggttca attctttgat ctttcgaaaa acagaagact
    19441 cactgatatt aaacgctgtc gctaatcgaa cgatggatac ttgctgatgt tctaataaat
    19501 agacgagcat cttaaacttc aagctttcct gcaataaaaa caatagaatt ttttccaaac
    19561 ttacatcttc gtccaacaca agcagcgcct gatactcatt tcgctgtact tctacttttt
    19621 tttccatcat tcggccaatc aattcaatat cttccaaata ttgatcaaac gaggcttttg
    19681 acagttggac atgctctctc aaaccattga tagaggctgt tcccccttgc agaacaaggt
    19741 ttcgcaaaat caggatctgt aactgctctt ttctttctaa taatgcttca atcttcatag
    19801 gcagtattcg ccacctttga ccacaccata tctggcacac ggtattctgg ctggaaaaat
    19861 agctcatgtt cttctaactg ttcaaacgtc catccttctt ctactgcaat tgtcagttgg
    19921 tcaagaaggt ggaacatctc ttttttgctt tggagctgtg ctccaagaat ctttttttca
    19981 gaatcaaata gaaatttgag tttaactttg ctttgattta ttgctgtaag cttgtagtct
    20041 acgtatcctg tttcaatttt ttggggataa aaaatgctct cttcttctgt caaacctacg
    20101 cttcctaaga aatagccaaa ccactgattg ccgactggac gataggttcc ctgatccttt
    20161 gtctgtgagc cactaattgt cttgctgaca tttcgtgctg tacggacagc gttatttact
    20221 agagaaacat acattttttc atttgttgga cgaaaagtta cttggatcgc atcgccaatc
    20281 gcataaacgt ctttttgaga cgtttgcaag tagtcatcta cccagatcgt tccatcatca
    20341 ttcaatgtta attgttcttc ccacatctga ttgtcgggac gagaatgatt agccagcaaa
    20401 acaagatcat agttctcctt tcttttttcc gtctctacaa tagcggtttc attttcggtc
    20461 aaagacacaa cttgttcttc taaaaaaagt gaaagccctt gatttttcat ttcctttact
    20521 aacggttctg tcatttcagg atctagataa cgaaaaagaa ttgactttcg gctttcgtaa
    20581 agatggattt gtttttttct tgatgccaac ccttcagcta tttcgattcc tgcttgtcct
    20641 gcaccaataa ttgcaatttt ctttgcattc atgatctttt gctgaagagc cgttacgttt
    20701 tgtatctttt taactgggtg aataagcgtt gattctgcag aaatatttcg aaacttttga
    20761 tttgaaccag tcgctagaat caaacgatca aaatccaatt tttttcctcc tgctaaaacg
    20821 actttttttt ctttcaatcc tattactgtc ttttctgtgt aaacatggat tcgatagcgg
    20881 tcagtcagtt cttctttcgt gatccaagaa agttgttttt cactggtaac ctttcctttt
    20941 aatatcaaat tgaacccgct gggaatccat cctaacttcg attgcttttc aatcaaatat
    21001 atttcacttg ttggcatcaa ttggcgaaga taaatagccg catgaatccc tgcaaacgac
    21061 ccgccgacaa ttacaatgtt catctgcatt cctctttctt ataaggtgtt ctactgctaa
    21121 cttctcaatc agaaaacaca gtattcataa ctcgctttca accttatgat acatagcgat
    21181 gcagcagaat caaccctctc catcataccg tgttctaggt ctgcttctca ataatgtact
    21241 agtggaccta attgaagagt atcatttctg aagagatgtt tcaaaaaatg tactcttata
    21301 aaagaaaaaa actcgccttt tacgtacctc tgtccaaata ggaaacagaa aagaacgaaa
    21361 agacgagttt tagcgtgtgt aatgtaatag ttagaataga cacatcattt atttttaatt
    21421 ctttaatgct tcaacaactg cttcgcctaa ttgatgagca gattgaggat tttgccctgt
    21481 aagaagccgc ccatcaattt gaaccacttc tgaaaaagct gctccagact gatagtttgc
    21541 tcctttttct ttcaaagcat cttctgctaa gaatggtact tcattcgttg taccatttgc
    21601 ttcctcttct tcattactga acccagtcac tgtacgtcca gctaaaatag attttccaga
    21661 ctcatctgta actggcaaca atcctacaac accgtgacaa acagctgaga ccagcccttc
    21721 atttcgatag atttgtgaag cgactttccc aagatctatt gcttctggaa aatcccacat
    21781 tgttccgtgg ccaccagcat aataaaccgc tgcataatca tttgcatcca catcatcagg
    21841 acgtaaggta tttcccaagg cccgatttct gaaatcttca tcttcataat aggcccaatc
    21901 gatttcatcc atattcaaaa cactttgcgg ttctaaaggt acatatccgc ctttaggact
    21961 gacaaaatct gcatcgatcc cattttttac taatacatca taaaaatgag tcagttcact
    22021 taaccacaaa cctgtaggac gctcgatctt ttcgtacttt tctacatttg ttaatacgat
    22081 caatactttc attttcccat cctctttcta taaaaattgc gttattgata acgtgctttc
    22141 aagaaattat ataatgggct tggcaaataa agctctttat atgcttcttc aaaaggtttc
    22201 gttacaaatt catcctcacg gttttccaat aatttctctg cccagtcagg atcgacgatc
    22261 atttgctgtc caattgctgc aagttctgca tatttcaata cctgctctac atcttcgcga
    22321 ttgcggatcc caccgacacc aactaaaggg atctttcctt gaatcgtgtt atggaccact
    22381 tcaagaatcg atttatcgtt gtatttatca gaacgagcta ttcgatcata catatttaat
    22441 gaaacatgca gataatctag ttttgactca cgtaatcgtt caagaagcca gatcgtgtct
    22501 tcaaaacgga tacctggtgt ttcatattct tctggtgaga aacgataccc tacgatgaat
    22561 ggttttttag catattgttc aaccgctgag aaaaccgttg ttaataattc ttcaataaaa
    22621 cgataacgat tttccaagct accgccccat tgatcctctc gtctattcga atgcggagag
    22681 aaaaattgtt ggatcaaata cgtatttgct ccatgcaact cgattccatc aaatcctgat
    22741 tcaatcgctc tacgtgtggc tttgccaaat gcttggattg tttcaacgat ttcttcttca
    22801 ctcattgctc gtggtgtttc cgcatcagga cgttttgctg caactgcact tgcagataca
    22861 ggctgttctc cagataaagt tgctcgactt gtcattcgtc caccgtggaa aatctgaata
    22921 atcgcttttg cgccttctcc ttggattgct ttcgctagct cacttaatcg aggaatcatt
    22981 tcatcatgag cgatgctgag ttcacctggc cagccttttc cgatatcttg gacatttgca
    23041 gcaccagtaa taacagcacc aagaccttta gaacgttgtt tataataagt gatctcatct
    23101 tcggtgatca ctccattgaa aaagctgagt tgtgtcgtca taggcgacag catcaatcgg
    23161 ttcttcaatt gaactcctga aggtaaagta attgattgat caaattgtga cattgtcatt
    23221 cctcccataa ttcgctctga ttaacctatc gagaatcatc tatcattaaa ccactcgatt
    23281 agttgtacat gcaactaatt tgcttaaaaa attttatttt ttttgtaact tatctgttgc
    23341 atcattcatt gtctgggcta attctttact tgcttcattt cccaaaactg ctttgtatga
    23401 ctgatgaagt tcttcccagc taccatcgac ttccttagca aattggcgtc cttgttctgt
    23461 aagataaact aatgacaagc gaccttcgct aactgttttg acgtatcctt tatattctaa
    23521 tttttcaaca aaacgagcaa tcgttgatgg ggcggaatag agcatttgtg caatatcttt
    23581 ttgtggtatt ccatcttttt cttttaatat caataaaata aaaccataag ttggtgccag
    23641 acctgtcctt ctaaacgctt catcagctaa tttattcaac atgcgatcta gctttttcac
    23701 gctaaagaaa agacattcat ctaacgcttt ttcaattggc ttttccattt catcccctcc
    23761 attggcgtac atatcatagt atgattttta ttttttgtca aaaaacacgg aataatgtcc
    23821 gcctcctctg tctagatttg aaaaaaagaa gtagccatct tcaaagaatt tcggcttgtt
    23881 ttcgacaaaa ctccttctgg aacaccgtga atcagaggtt gtgaaaaagt cgtttagttt
    23941 tgagtatgaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa atttggctta
    24001 ataccgaaaa actggctttt gaacaccgtt tatatcagag gttgtgaaaa agccgtcctg
    24061 catcaagtaa taagaacagc caaacaaaaa tagttcctca tattttgtcg ttttttgagc
    24121 taatgccgat ggtcagctga ttgtacaccg tttattgaga ggtcgtgagc ctgccgttga
    24181 actccgagtc acaaggagca atttcaaaaa acagcttctc atgttttatg actatggtga
    24241 cttgtgatcg aggagttggc aggagaatac tgtttattcg taaagaaggg gctgtgacaa
    24301 gacttttgtc acagtccctt atttcctttt attataggat ttcgccattt gattggatga
    24361 cttgtctgta ccaatcaaat gattttttct ttaaccgttt ctttgtgcca ttcccttcgt
    24421 catcttgatc aacatagata tatccataac gttttgacat ttgtgaagta gaagcgctga
    24481 cgatatcaat gcagccccac gacgtataac caaataaatc tactccatct ttgatagctt
    24541 ccttcatctg ctcaatatgc tgcttcaaat agtcaatgcg gtaatcgtct tgaacggttc
    24601 catcttcttc tactttgtct atagctccga gtccgttttc agctatgaac agtggttttt
    24661 gatacctatc atataattga ttcaaagcaa ttctcaaacc cttaggatca atctgccagc
    24721 cccattcact tgatggaaga tacggatttt taattcctcc agttattaag tttccttgga
    24781 ccccttccca attttctgga tgagcactga ctgcactaga catgtagtag ctaaaagaaa
    24841 caaaatcaac agggtaggct gctaagattt cttcatcacc tacttctatt tttaagttga
    24901 cacctttttc ttcaaacatt cgttgcgtat acgctggata atatcctctt gcttgaacat
    24961 cactaaagaa caaagaagtt ctttgctgct caattgtttt ttgaatatca tccggatcac
    25021 aagaatacgg aacaagaagc tgatagctga tcatacaacc gacttgtgaa cctttgataa
    25081 tttcatgaca agcttttgta gctaaagcac ttgctataaa ctgatggtgt gcaccttggt
    25141 atttcgcttg ttccaaatgg ggatggtttt cttcaattac tcctacgcta acaaaagggt
    25201 gatgttttac acagttgatc tcattgaaag tcatccaata ttttacttta tttttatagc
    25261 gtttgaatac tgtttcagcg aaacgaacgt aaaactgaat aagctttcga ttaggccatc
    25321 caccatattc tgtaaccaaa tataacggca tttcataatg ggaaagagaa acgactggct
    25381 caatcccata ttttagacat tcgtcaaaca cttgatcata aaaaaataat cccgcttcat
    25441 tgggtgtttc ttcatcacca tgaggaaaaa ttcttgtcca tgcgatagac atccgatagc
    25501 atttaaaacc catttcagca aataaacgga tgtcttcttt aaagtgatga tagaaatcat
    25561 ttccgtgtct tttaggataa taatacttgc tgttgggatc ctgcgccgct tttacttgct
    25621 gatgcgtcat catatgccat tgatctgtcc atttttcttt aggtaaatcg ctgtcgaatg
    25681 tatacacatc agacacagat aatccttttt catctacatc ccatgcacct tcacattgat
    25741 tggccgctgt cgctccgccc cataaaaatc cttctggaaa ttttttcata tcgatccccc
    25801 taaacttgct agttttcttg tttgattatt tttcttatga ctagtaatga tatttttgaa
    25861 tctactacta tttgtaacaa taaccattgt ggtcggatca tatccctctt tgattaattc
    25921 ttgcaaatct acttccatca ataaatcgcc tttacgtact ttttttccat cagatactgc
    25981 actttgaaag tatttgccat ttaaattcac tgtatcaata ccgatatgga tcagtatctc
    26041 tgctccttct tcactgacta ccccaattgc atgtttcgtt ggaaaaactg ttgaaacttc
    26101 cccgtttaca gaactgtaga tttttccgtc cgttggaatg atcgcaaacc cttctcctaa
    26161 agatccacta gcaaatgttg gatcgtttac ttcagataaa tcaattattt cacctgctgc
    26221 aggagtactt aacactatat cctttgagga ttcttcttcg atttcatcct tgtagccgaa
    26281 aagaaaagtt agcatacagg aactgatcat cgctaagaaa taactagcaa ctagccaaac
    26341 aaatccttca ccaatataaa tcgggaaaga caaaatactt cttgtagcaa ctgccaaagc
    26401 accggctcct gcggcaccag ctactccacc aaatatagct ccacatatac aagcaatgta
    26461 aaatggtcgt ttatatttaa gagtcacacc ataaacagca ggttcagtga ttccaaataa
    26521 tgctgtgata aatgcagata aagataaact ttttactttc ttatttttcg ttttcaaaaa
    26581 taccccaagt actgctcctg cttgcgcagc tacggcagtc atacatgctg gacctagcgt
    26641 atcacgaccg tagaaagaaa gattgttggt cattactggt acaagtcccc aatgtaaccc
    26701 aaaaatcact aacacttgcc aaaatccgcc gataacagca ccagataaaa cagggctaaa
    26761 gttataaaca gctaaatatc cagctgcaat cagctgactt gcataatctg caattggacc
    26821 tacaactaat aaaataattg ggaccatcac gatgatacaa atgaatggtg tcaataaatt
    26881 tcgaatactt tcgtgcaagc ttttgcgaat cttaggctca atataagaaa gaacccatac
    26941 agctaaaatt gctggaataa ccgtacttgt ataccttgct agaatcacgg gcatccctag
    27001 aaatcgcaag gttatgctat cactatatgc actgacaatc gtaggataaa cgagagctgc
    27061 tgcaataacc atagcgatga aacgatctgt attgaacttt tttgcagcag tatatgccaa
    27121 aaatataggc aagaaataga aaaatgcatc agctgctgca ttcaaaatga tataagttcc
    27181 tgattcagta gttaataaat tcaaactcgt acataaggcg agtactcctt tgatcaaacc
    27241 acttcctgcc aagacgccta aagtcggggc aaaaacagcg gcgatcatgt ctataaaaga
    27301 gtttaaaatg ccttttttct cagttcctct ctttccacta gatgcatcat cttctagttt
    27361 catttgtttg gataatacgt catacacttc atttactgca tttcctacta cgacttgaaa
    27421 cattcctcca gcttcgacta cagtgatcac accttctagt tgctctacct tctccttatc
    27481 tgccttctta ttatctttta gggtaaagcg taaccttgtt gcacaatggt aaacattgct
    27541 gacattttct tcgcctccga caaatcggac aatatctttc gctaattgtg aatagtccat
    27601 ttttacttcc cccttgttaa ttaatgccgt agcaatcctt cttacaagat aagtatagtt
    27661 cttagaaaac ggtttctcca ttcaattagt accccttcta atagcgaacg aaactgtaca
    27721 atagtgtgta tggtttactt agccgagcaa atcactcact gcttgaaaga aaacaagtat
    27781 atcgtttctc ttatagtcaa aggagacaaa aaaaacacgc ttgatcttcc tttgctgatg
    27841 tataagtggt atctcaagct gatactctgt gatttttgtt tctacttggc ttttgataag
    27901 acagcacaac aaagtctcgg catttaacgg aagcataact aggtcagaac taggagagat
    27961 tagttgctga atggaatcta tctttttatg gatttttatt tgattttctt ctttgtagta
    28021 atgatcaagc agttttgttt tctttttttc ttcaactatg gtcagctttt cacgcaaaat
    28081 ctgataaatc tcccaagatt caaactcatc tagaacacta ggtagataaa tcaaatcatc
    28141 ccactcaaaa ataagctctt gctcagtagt caaaattgca tcatattccg ttattttgtt
    28201 tgattgattt ttgaaaagat acactggttc tatccggcat tctttgatcc acatgttcat
    28261 actttgttga atctgttttt ttagtgtatt caaaatacta attggctggt tactaacaat
    28321 cagtatctcc ccttgttttc gaaaaggatt caacgcttcg gcaagataca aaacaaaata
    28381 agcgctttca gctaaattgg gacgtaattg aaaatgtatt gggaagaaac gccgaattaa
    28441 ataaatttct aaaggataac gggagatgat cttttgtaca aaatgattca tggtattatg
    28501 tccggcatcc atcctcgttt tcatttgttt gatatgggtt agcaaaaatt ctgtttcatg
    28561 gaacaataac gaaacaggaa gatctaaaaa acgaataatc gtttgctcaa atgctcttag
    28621 attcttctct atttctttgt ttttttcatc attaatcatc aaataattta actctaatag
    28681 tcgattttca attgcttgtt tttcttgatt ggaaaaagaa tagggtagtt ctgatgataa
    28741 cttttttgtg agggtctcta ccatcggaat aaataatgta tacctgtttg ttttttctaa
    28801 aagttcttct cttcgaagaa tggtcaaaac gagataccga gaaaatcgta tgaagtcctc
    28861 tcctgaaaca atataattat atgatataag aacctctttt aatgtctttt ggacagtcgg
    28921 catgatattg gaaaaaattg tactgatttg ttgaggaaac cttgcgtgtt ggatcaccgt
    28981 ttctgttcca acaagagata agaaaattct ttttgaattt tcttcagcaa tgatttttag
    29041 tccttttgtc gacgaaactt ccagttctaa tttaggattg cgctcgatcc aacgcaaaat
    29101 cgtttttatt tgtaatgata cggctgtttt tgaaagaaag aaaatgtctg ctatcttttg
    29161 catactgaca tacccttttt gaaacaataa gtacgtcaca atagttgaag ctcggaaatc
    29221 catgctggaa tagattttat gtttcgtcac ctcttgtatg atttgctgtt tagtttcaga
    29281 ttcaattgat tctaaaaaat aacctgaacg tttatttgac tggataatga ttttcttctc
    29341 gtagatagca agcatatttt tgatttcagt ttggatcgtt ttcttgttgg tacctaaagc
    29401 ttcagctaat tcttcgcttg ttatccaatg attcttattt cttaatacta caagaatttg
    29461 gagttgtcgt ttatttaagt ccatttgctt acacctcctc tttgttataa acatacagga
    29521 aaaacctctc aaatgacaat agtcacttgc agaggtttaa aaatcatttg aagaaagatc
    29581 tatattcaag gtttttacca ttagactgga taatttgttt ataatattca aaactatctt
    29641 ttttcaatct cctaaagctt ccgctcccat cattttgtct atccacataa ataaagccat
    29701 atcgcttcct catttcccct gttcctgccg atacgatatc gattggtccc caccataagt
    29761 aaccaatgat ttggcaacca tcttttattg cctcagcaat ttcctgaaga tgtattttta
    29821 gatactcaca acgttttgga tcgtggattc gattcaattc atctggtgct tcatctagtc
    29881 caatgccatt ttcaactatg aataatggta attcataacg gtctgtcaat tcattcatga
    29941 cataacgaaa ccctagagga tcaattggcc aactccaagg ttcgggagat ttttccttca
    30001 aaaagggatt atcgaaacca tttgctccgc cagtatctac gcgcatttct gcttcttttt
    30061 tatagacagc gctacgataa taactaaatg caatataatc tgccgtgtta gcttgcaagc
    30121 actgttcgtc tccatctaac attactggtg cacagtcttc ttccttccat gtcctgtaaa
    30181 tataagctgg ataatgccct cggcacatga catcggagaa aaaccatgaa ttgcgtttaa
    30241 attgcagtga accaaataca ttctctggat cacagtcata tgcataagtc gctaatgcag
    30301 aaaagctaca cataataccc atcttagcat ttggatcgat tttctttaat aattgagtag
    30361 cacgggcgtt cgctagaaac atatgatgac aggcttgata aatcactttt tttgttagtg
    30421 tagcaatggg ttcaagttta tctctagcag ggaatgtaac caatccagca gctgcataag
    30481 gcatacgaaa agcattattg atctcattaa aggtcatcca atatttcact ttcccttggt
    30541 agcgttcaaa gacggtctgc gcataaaatt caaaaaagtc gattaatttt cgattttgcc
    30601 accccccata ctctcctacc aatgctaagg gggtttcata atgactaaga gtaattactg
    30661 gctcgatatc atatcgattt aatgtattga ttagtctgtc ataaaatacg agaccagcct
    30721 catttggctt ctgttcgtct cctcttggaa atattcgtgc ccatgagatg cttgttcgaa
    30781 aagcttttaa acctaactcc ttcagtaaac gaatatcctc ttcaaatcga tgataaaaat
    30841 caatcgcttc atgagaaaga taatggagtg attcgtctaa agcaatctcc caaatctttt
    30901 tttcttcatt ccactgaata ctaggatgct ggttcatgat cccgataagc gtatctgtca
    30961 cattaggtaa tttgccgtct tccaaccatg caccttcaca ttgattagca gctactgctc
    31021 cgccccataa aaaattttct ggaaatattt cctgatccat aactatctct cctcgctatc
    31081 aacaatttct tacttgaagt atagctcgat tcatcaaaac aagatattct aactatttcg
    31141 gcagaagaga agaaaaaaat tgaatgctac tcttcatttt taatatttat atataacaaa
    31201 tacataagac gatccccgaa agaaaacact cgttaaccat ctaagcaaac gatttatttt
    31261 acttccaaca caaatgcttc ataaggtcgt aaatcaactt tgccgtctgg taactgtttc
    31321 agaggataat ttgacacaat aacccttaaa gcttcacgtg gaatagtgaa cgtctgcttt
    31381 tcactggaca tattacaaat aacgagccat tgctggtctt catatatgcg agaataggca
    31441 aagacatgct caggcgtctt ttccactaat tcatagtctc cccagacgat gatgtcatgt
    31501 tttctacgca aatcgatcaa tttcttgtac atataaaaaa cagaatcttg atctgctaac
    31561 gcttcttctg cattgatttc tttataattt ggattgactc gaagccatgg aattccagta
    31621 gtaaacccag catgttcacg ggcgttccat tgcataggcg tcctagcatt gtctcttcct
    31681 ttcgccttga ttgattccaa gatttcctct tttgtaaatc cagaagaaat cctttcgtta
    31741 tacatattga ttgtctcaat atcttttgct tccgcgatat catcgattgg gcaattcgtc
    31801 atgcctaact cttctccttg atagatataa ggggtgcctt tcatcatatg gagcaagata
    31861 gcaaacatct tggcactttc ttttcgatac acatcgtcat ttccccaacg agaaacaatt
    31921 cgcggaagat cgtgattatt ccaaaataaa ctattccatc cttcatcacc taaagatgtt
    31981 tgccatttgg ataatacttt tttcagttca gagatactca agggtttcaa atcccatttg
    32041 tctttgcctt cttgctgatc taatcctatg tgttcgaatt ggaagaccat ggataattca
    32101 tttcttttag gattggaata aagtttagcg atttcggtag ttgctcccca tgtttcgcca
    32161 acagtcatca gatttttgtc accaaaagtt gcttgattca tttcctgaag atactcatgc
    32221 agtttaggtc cattacttgt gatttcttta tccggttctt tgccaatcat atcgatcacg
    32281 tccatacgaa agcccccaat acctttatcg atccagaaat tcatcatttg ataaatctct
    32341 tggcgaacct gtttattttt ccaattcaaa tcaggttgac gcttgctata caagtgcaga
    32401 aaatattgcc ccgttttttc atccaactgc caagcagaac cactgaatat tgagcgcaat
    32461 ctattaggct cttttccatt gaccggatca cgccaaatat aatagtcccg ataaggattt
    32521 tctttatttt tacgggcctc aatgaaccac ggatgttcat cagaagtatg attcaccacc
    32581 agatccatga tgatacggat atttcttttc tttgcttctt caatcagttc ttccatttct
    32641 tccatcgtac cgaattctgc catgattgct tgataattac tgatatcata cccattgtca
    32701 tcattcggag actcgtatac tggagaaagc caaattgcat caatacctaa atacgccaga
    32761 taatccaacc tggaaataat cccccgcaaa tctccaatac catcttgatt tgaatcttga
    32821 aaacttcttg gatagatttg ataaacaact gaatggtgcc accatttttg ttccatgatt
    32881 ttcctcctat aaatggatgt ttctattttt tgcttaatca tttattttag tctctctcac
    32941 taaaaattgt aacgctttca aatagctatt gcaccttgtt atcggtaaca gaataaaaca
    33001 aaaaaacagc tgaggaaatt ccctcaactg ttttttgaaa ggattgattt atgcagggaa
    33061 gaatgctgat ttcacaactt ctttataaac ggtgttcact cagctgtccc ttggtattag
    33121 ctcaaaaaac ggcaaaatat aagaaactat tttttcgttt ttttctctaa tactcaggcc
    33181 agaacgctga tttcacaacc tctttatttc acattcaata tccacatgaa tcccacaaat
    33241 acaaagaaca gaccatacat gattggagat acttcttttc ctcttttagc tgctaacatg
    33301 gtaattggat agaagataaa acctaatgca atcccatctg aaatactata agtcaaaggc
    33361 atgccgatca agatcaagaa tgaagggatc gcaatctcta agtttcccca ttcgatttgt
    33421 cgcaaagatt gtgccatcaa tactcctacc ataatcaatg ctggtgcagt tacttgtgaa
    33481 gtaacaactg atagtaatgg agaaaagaac aagcctaaca agaagaaaat ccctgtagta
    33541 atagctgtga tacctgaacg tcctccaacg gcgataccag ctgatgattc tacaaatgca
    33601 ccaacaggag aagtaccaaa taaggaaccc gcaagcatag ctgttgaatc agaagctaaa
    33661 gctttcccta cacgaggcat cttgttgtct ttcatgaaac ctgcttggtt tgctaatcca
    33721 actaaagttc ctgctgtatc aaagaaagtc acaagcaaga atgtcagcac tacgacccac
    33781 atttgaagag aattgatgtc ccctacatgt tttaatgcaa ctaaaaatgt tggtttcaaa
    33841 cttggtgctg cggcaacaat ttcagaaggc attggaatca aacctgtgac cagacctaaa
    33901 atcgttgtag ctgccatccc gatgaagatg ccacctggta cacgccgaac aagcaagata
    33961 gcagttacga ctaatccaaa aatcgtcaac caagtagaac caacgttgag tgatcctaat
    34021 cctactaatg tagattcatt cgaaacgatg atcccacctt cgctcagacc aaggaaagcg
    34081 ataaatagac cgataccgcc agaaatcgca tatttcaaat ctgctgggat cgcatcaatg
    34141 atcatttccc gcagcttgaa gattgtgatc aaaataaaaa tcaaagaagc aacgaatacg
    34201 ccggctaatg ctgtttccca tggaatcccc attcctaaac aaacagaata agcaaaaaaa
    34261 gcattgatcc caagagcagg tgccgttcca attggataac gggctaatac ccccattaaa
    34321 atacagccga tcgcactggc taatgcagtc gcagtaaaca ctgctccttc atccattcca
    34381 gaagcaccta atacagttgg atttacaaat aagatataag ccattgagat aaatgttgta
    34441 aatcctgcca gtacctcacg ttttaccgtg gtgttcaatt tttctatttc gaagtaggaa
    34501 agtatttttt ccttcatctt ccatccccct aaatccgaac attataaata aagtccgtta
    34561 aaatttcgat ttattaataa aacaacgtgt tttatcatat actaaaacaa aatataagta
    34621 aacaaaaaat tcggatttta agcaaaatcc gaacgtttca tctgattaaa tccctttatc
    34681 tcaaagttca tgaacattca ctttggatta caagaatttc tttattgtgt taataaagtt
    34741 tcttcattct agtctcaaaa atcaatagtg tgatttgatt ttaccatttc gtttctgtta
    34801 ttctttgatg aattcttttg tatcgacagg attcagatta tttagtccgc caaaacctgt
    34861 gtcgaccatt tttccccagc cttttgtctt cttttttcct gatttgagat tctttgctcg
    34921 aagcttctca ctatgttttg ctcgccgata cttcaaataa ccttctaatc ttcccattag
    34981 atccactgtt tttcctcctt tttattcaat cagacgactc agcttttcct ataacaaaat
    35041 aattgccttc agggtccgaa aaattgaaag tcaagacgcc attgttgtct gtgatttctc
    35101 ctggttcttc catgttctcg tagatagtct caaattcgtc cgtaaaaaac actaaagaag
    35161 gcggtggtcc taatacttct ggtgaaaatt tctgcacaaa tacttttggg aatacgccta
    35221 attctaattc ttttgagatg ctgagtacaa tactcttaaa ttcttctggc aattcgattg
    35281 tttcagaaat ttctgcacca attttttcta caaagaatcg gcaaatcatc tctacgtcat
    35341 tgacataaag catcacacgt gttttttgta tcatcgtttg acccttcttt ctatttgatt
    35401 ttctttcctg tctataaact gattccatta tatcacgaaa gcgcaaacac cttgtgtttg
    35461 atactattca agaaaaataa agcaatagtt tctaggattg ctttcctcca caaacttttt
    35521 ttacaaaaaa aaaaaaacgg caatgaatgc cgctcttctt ttttcgattc ttttataatt
    35581 ctgcttctga ttcatctttg gttagtgtgt gtctaacact agttacgcca aatgtttgat
    35641 cgattttttc cttcaacgct tcgattgcct gtgcgccacg tgccgcttgt tcagcattga
    35701 tggattcaat gaccagcacc gctgcttgtg tttcttccgt cagcatcgtt ctttgggctt
    35761 cacgccagtt gagacagcga cagatcgctc cttgttcatc atagtagatg atttcttccg
    35821 gaagtgccgg agcatcggaa tctgcaccta aaggcaggaa tgattcgcca ccttgggctt
    35881 ttcctaaatg cagatttcct gcgatctttt cgatatcttc tccgccgcaa ggtacgccaa
    35941 aagacaagga aatactatta tagatatcta caagcggatt gattgggaaa aattcccgtt
    36001 cttgatttgc acgtttcagt aaagcttcaa tagaagaacg tgcacctttt ttagtcttga
    36061 atgtggtaaa tgcctgacgc cactgagcaa tgacttcgtt ttggctgaat gtttcttctg
    36121 taaggtaatt tcttgcttct tttgctgcct cattcaataa ttgatgatac gtttcttcac
    36181 tttcttttgt tacgtggtta tcgataccat ttacggataa gatttcgatg gttgcttcag
    36241 gaaatacttc ccagaaattg gtatccacta ttactttttt catatgttca cacctcttga
    36301 ttagaataat tgcttgatga atagatagtc tgtttgggga tcatctccca tataaaaaac
    36361 atgttcgctg tatttgataa atcctttttt acgataaaaa gcgagcgcaa catcgttgtg
    36421 ttcccaaaca ccgagccaaa tggctgtttt ttgcttttct tttgcttttt caatagctgt
    36481 atctagtaaa aatgtaccga taccttgatg tttaaatttt tttcgtacat agattcgctc
    36541 gatttctaaa aaatgatcag ccatttcctc agattgactc gttcctgtat ttagtttcag
    36601 gtaacccgca ggctcttgat cgatactcac taggaaaaaa tcggtttctt tatcttttag
    36661 ctcttctgta agtttttctg gcgcataagc ttgtgtcaag taatcagcta aatgcttcgg
    36721 tgaattatct tttccaaatg tatctgtaaa tgtttcgata ctgatttgtt gtagtaagtc
    36781 tacatctgaa ggcgtcattt ttcgaatttc gtatttattc acggcttctt cctttcaata
    36841 gatcctttta ttgccttttt tgacaaaatt ccaatcttcg ctaacattct tgcatacttt
    36901 atttaaaaga tattccaatt gttttgcttc ttcgtcagaa agaccattta atgcgactgc
    36961 attggagtag ttgttttctc gaatgatgaa aggatagatt tctgctcctt ttttagtcgg
    37021 atagagtttc ttgatttttt tattttcttt atcttccaaa cgttcgatca tcccattgga
    37081 ttctaatttc ttgattgctc gggcagtcgt tgtacgatct actttgatca tttctgctag
    37141 tttttcttgg atgattccag gattttcgca aatacgtact aaataaagat actgtcctct
    37201 tgttaaatcg acttctttga attcgatatt gctgatagaa tctagcgctc gggcaatcat
    37261 tccgatgtct cgaaggattt cagccatttc atcccacctt tattttgttt gtaagactag
    37321 tttacctctt tttgttgcaa atgcaactat tttcaataaa aaaatcaagt tcttttttaa
    37381 taactagtaa agtatattct tttcttcttt tacgaaaaag ctcaatcaca atagatctgg
    37441 tttttgtttt ctaaaataag taactgtttc ttcgttgttc gcttttctga tagctttggc
    37501 aactcttcta tactaaaaaa tcccatgttg gaagtttcat tattctcaat aaacttggcc
    37561 tcttcaccgt ggatcgcaca agcaaaaaca agtttataat actgaaaaag ctgtggaata
    37621 tctttttgtt tattcgtatc aaaaactgct cgcaactgag tagtttctac tgttaatcct
    37681 gtttcttcat agacttcttt acgcacattt tcttcaggag acaatcctat ttcagcaaat
    37741 ccaccaggaa gtgaccattc tttcgtccgt ttatcttcga ttagtaggac cttgccatct
    37801 tttttgatat aggcacgaac atctactttt ggtgttggat gtccttctcc aatcgttgtg
    37861 agcgctttta tttcttcgaa agtttctttt cctatttcac tgatcagttc caatgaaatc
    37921 gtccggatct cctcaaaacg ctctttatca aatacatctt ttccataata tagaccagct
    37981 tgagaaagtg cgagtagtct tttgtattga gccaacttct tgttcattct cttcccttct
    38041 tttttagcaa atgtatgact atgtaatcaa gtatagaaga ctagtttaat tacggcaaat
    38101 aaatataatg aaatctgcaa taaataaaag aatgttaaaa aactcgagaa caaaagacat
    38161 tcgacttctc tggggctgtg aataaatggc gttcaaaagc ttgcatctgc ggtaataagc
    38221 cccaccaaac gaaaaatatg acgagctatt cttgtctggc tgttcttatt aattgaggca
    38281 ggacagcttt ttcacaacct ccccactaaa cggtgttcaa tcagctgatg ctcggtattc
    38341 actaaaaaaa cggcaaaata tgaggagcta ttttgccgtt tttttctata atactcatat
    38401 taaaacactg acttcacaac ctcttcctta aacggtgttc tcctaccaac tcctcagtca
    38461 caagtcacaa tattcatcaa acatgagaag ctgtttcttg aaattgctcc ttgtgactcg
    38521 gtgctgaacg gtaggctcac gacctctcgg taaacggcgt tcaaaagcca atttttcgat
    38581 attaagccat ttctttgtta cttattgtca ttgatttttt ttctaattta tccaatagtg
    38641 gagtgtaatc cacttgagta tagtctgtta catcaaattc atatagtgtt cctaactgga
    38701 atttgtcata ttttcggtcg gcgatgatct ttttgaatgc ttctttcgta atatgattgg
    38761 tccctttcgt tcgatcttcc aatacgtcac catagtgata atgatccatg atatagctca
    38821 aatgacgatc tgattcacga tcacgtttgt accgacgttg ccataacact tcaaagtctg
    38881 ctgttaggcg aatcgtgatc acctgatatc cgtatctatc tgctaattct tggagtcgtg
    38941 ctttttgctt atcactaaaa ggatactcgg acacaatcac tttctttcct gcttccatat
    39001 acaaatcaag tgctttgtaa tagaaaggat aaactttcgt cctttcttgt tccgcccgtt
    39061 cttccaaatt gttaaacccg atggattctg catagtattc cttgatttca tcgagcgtga
    39121 tggcatacat atctggaaat cgttcccgta gtttattcat aagatatgtt tttcctgtac
    39181 caggactacc agccagaaga atgaaatact ttttcatctc atttatttcc taagttaatt
    39241 ggactttcca agttcataaa cagtccaatc ttatgcttga caatttcttt tactgcttca
    39301 tttgctggaa tcacattatg gcgaagagac ttgttgattt ctgtttcact aaaggtttct
    39361 ttagctactt tcgtccagtt gactaaaagc tccgttccaa cattgaattt gcggatcccg
    39421 ttattgatca attcaggata atcttcttct ttcacacctg ttcctccatg gataactagg
    39481 gggacatcaa ctactgcatc gatttctttc agcaagggaa tgtttacttc tgtttttgat
    39541 ttgaattgtc catgattcgt accgatagca atagctaatg catccacgcc tgtagcttct
    39601 acaaatttca ctgcgtcttc cggtcttgta tataccttgt cattttcagc gacatggatt
    39661 ccttcttctg taccgccgat tgttccaagt tctccttcta cggaaacacc gtgcgcatgg
    39721 gcatattcta ctactgcttt tgtcttcagg atattttctt tgaaaggcag atgggaccca
    39781 tcatacatga cagaagtata tccgctgtcg attgcttctt tgatatcatc aaagtctcgg
    39841 gcgtggtcta agtgcaagac tgcatcaacc atttcttctt ctgcaatagc tgtcattaca
    39901 ttgactaaca catgcatgct gatatattta gcagtatcga cacttgtttg aataatgatt
    39961 ggcgaaccca tttcttttgc tgcgcctagc atggcaggaa gcatttctag gttatgcgta
    40021 ttaaaagctc caactgtaaa attcaactct tctgctgttt ttgttacttc tttcaatgtt
    40081 gtatacatct tttattgcca ccttatcttt ttatttgttt cctttgatcg ttacttgttt
    40141 tgcaatttgt cgcatctcat ccattttgcc catgtagtaa tcgatccctt gatcatcacc
    40201 agcacgagca gcatctgaac gcttctggtt atagaggctc atcagttttt tatacccatc
    40261 tccgatcgtc tgtttatttt ccaagctctt ttctaaatat tggatggctt gatcgttctc
    40321 atacaattca gaaaatttaa gtccaacttg ttcataaagt tccgctaatt tatgcctatc
    40381 ttgggtagtg gaaatctctt gtttcaattg tgtaatctct gattgaatct tttcggtcgc
    40441 ttctggcgat agggcttttt cttcttttgc cagaactttt ttcggttctt tttctttttt
    40501 cttaaaaaat ccaaacatca ttttccctac tttcaattaa aatgctttca aaactgggct
    40561 taagatccag gcatataaga gtgctgcggc tactgtatct acatcggttg ctgtagcatt
    40621 gatgaatccc atcgaattaa agatcgttac tagcaaagcg ggcaataatg taataaagaa
    40681 accatgagca ataccgccaa tgatcgctcc tcggcgtccg ccaactgcgt tgccaaatat
    40741 ccctgctgtc cctccggcaa agaaattggt tagcatacca gataaaatca tcgctaagcc
    40801 gaacatgggc aaagtaaaca tggcaataat cgtaccaatc gttgtcgtaa tgaaacctaa
    40861 aatgacagca tttgggctat aaggaaagaa aactggacaa tctaaggcag ggatcgcatc
    40921 aggaactaat ttcatagcta tccctcgaaa tgccggcacg atttcaccta acagtagacg
    40981 tacaccagct agcaatacat acacacccac tacgaattgg atggcttgta aaaaggcaaa
    41041 catgatatag ttttgatctc ctgataattc agaagcgaat ggttcgcctg caaataaaac
    41101 agtgatgata tatagcggga ccatcacgac catgacagac aaataagtat cttgcagaaa
    41161 ttcgaaatgt gtcggcaact tgatatcttc gatcgacttt ttattttcgc ctttctcacc
    41221 aaaaagctta gcgacaccgg cttcaaataa gtacccgatc gtacaaaaat gacctaaagc
    41281 aatatcattt gatccagtga ttttacgaat gatcggttgc gctactgccg gcatagcaat
    41341 ggcgaaacaa gctccgataa aaccaccgac caagatcaat acgatccctc gcagaccggc
    41401 aaaatagcca aagactgtcg tcatcgttgc catccataaa attgcttgac cattgataga
    41461 ttcaatagaa gggatgatcc cttgcatatg aaatccttca gtaaagattt tcccgaaata
    41521 agttaaactt ccaacgatga tgctcgaacc ggcactcaac acttgaaatc ctaggagtgt
    41581 ctttaacgtg ccagagatga cttgtcctgc tgatttcttc tgcagtagta gacccaacat
    41641 agcaatcaat gcaatggtga tcgatgcctg agtcaaaata ttttcgatga taaaattgat
    41701 aacgctcatg tttttaccct cttttctttt tgtatttaga taactccctt atcttgcaga
    41761 actggggtga ttttttcttc aatttcattt tttgaaacaa tatttttcaa atagatcatc
    41821 gccgttttag gatcaactgc gaacttttcg aactgcgaac gaaaattttc tgctgtgatg
    41881 atcacatctg gtttcatgga aactgcggaa gaaatatcac aatggtccag tttggcttct
    41941 actcctaatt tgttcaaaac atcctcagca gacatctgtg ctgcaaaact acttcctaaa
    42001 cctgcgccgc atacgaataa aatgttcaat ttgttcatct ttgttctccc ccgtatcatt
    42061 tattatttct caactactga aaaaagcagt tgtttgaaat cttcgatatt tgtgcattca
    42121 atcaatcgat ccaacttctc ttcgttgttg atcaattcaa ttagttcttt catgatgttc
    42181 agatgcgagt aggagtctac tgctgccaaa caaaaaataa tctttactgg atccatttca
    42241 ggattaccga aatcgattgg ttgctcaatc gtagctacgc tgactcctaa tttattgacc
    42301 ccatcttctg gtcgggcatg ggctagtgcc atgtgtttgc caatcacgat atacggtccg
    42361 taatcattca ctgcacgaat catcgcttcg acatagcgct cttcgacaat gttttcttta
    42421 accaatggtc tagcagctag ttcaatcgat tcctcccaag tagtggcttt ttctttgatc
    42481 atgatattgc tgtccgtcaa aatatctttt aacataggct ggatctccct tttgttgatt
    42541 tctaaattgt ttgtttcgaa taatttttct aacttgccat agatgattcg atctactcgg
    42601 ccaccacttt ctttcatgac atctattatt tgagtgaata actccgtcga ttcatgactg
    42661 ttaggctcta accgctgtag ttcttcattt cttttcaaaa agtcttctat aaccaagata
    42721 ttcgtctcag ttaaaatcgg atcgacaact aaaagcggct tatttcgata attcaattga
    42781 aaggtgctga aaaccagatt gacatccaat ttatctacta attcgatttc ttttgaactt
    42841 aaaactgcag taatctcgat ctgaggaaac ttttctttca gattctctgc taacagattt
    42901 ccagtagcca tcccatggtt acagacaacg actgatttat aaacataatt caagtctcgc
    42961 ttgattacac tggcgatcgt caaaaagtgg atgacaagaa aagctatctc gtcctccagt
    43021 atcttgccac caattacttc ttcgatcgta ggaacaaaat gagtgactgc gcggtagatt
    43081 gggaaataat ttgttcggat gttttctttc aatggattca tgacttggat atgattctta
    43141 caatcagtgc agccatgtgc ttatagagat tttcgaataa agcttcttcc tttagatgga
    43201 aaggtacgtg tgtctcgttc tctacaaatt ggatcaattg gatcgttagt aattgagctt
    43261 gtacccactc gattgaattg gagatatctc tagcattgaa cgtctcaata gtgaaacgaa
    43321 gataatttct ttctacttgc gggacattgt ccaattgaaa ttcctcgatc atgtggtcaa
    43381 taaatgcaga aaagctactg tcttttattt ctgtattttt ccaattaatg gcagagataa
    43441 attcatgttt cctgattcgc tggatccaga tcaatgtaaa aagcaggatt tgttttttat
    43501 aaatatcatc ttcttgtttc aacaacttat tggaatagat ctcctgtatt tttttgattt
    43561 ccaccaaagg aaaataatgg agaaagattt tttgagccgg tgttaattgt gcatcttgat
    43621 tcataaagaa gtcggtcatt cccagattct gattgataaa atcatagatc atcgtacgga
    43681 tagatcgttc tgaaccttta taaaccagtc cttgctttcc ataactgacg atctctatcc
    43741 cataactgac cagtgttgct cgtaaccgtc tcatatcttc atctaatgta cttttagaga
    43801 tttgaaattc ttcttctttt ttgtttagat ggatcgggat tcggctgtaa gcaacggcta
    43861 aaactagatc aaaaatacgt tcgcgccgat tcaaaaagcc gtctttcaca tcttttattt
    43921 cagtttctaa tcgagcttgc ttttcctttt ccagcttcaa caaaaaacct ttgttgcgaa
    43981 ttgtacggat ctccggaagc tttttactgc tcaaccagat attcgcttca agaacttcct
    44041 gacggatcgt ccgctgactg acattgaact cacttgctag atgttttgct tttactggat
    44101 attgggctgt catgattctg tgaagtattt caattgttct tgttttcata gaaatgatct
    44161 ccttctgatt tcaagtatat ggttccgctt acataataaa aagtagatat ttctgccact
    44221 tgaagtggaa agaaagtaag caaagcaaac aaaaaataag ccatatgctt tttcatctcc
    44281 tcaacgaaaa agacatacgc ctattattac aaaaattttg cccatccata caaaaaagag
    44341 gttgtgaaaa agctgtcctg catcaagtaa taagaacaac taaactaaaa tagtgcttcc
    44401 tattttttgt ttggttgttc ttattatcgc agatgcaagc ttttgaacac catttagtgg
    44461 agaagttgtg acagagatgt tcagccttga gcgataagaa gcgattttga aaaatagctt
    44521 cccatatttt acaaaatcgt gccttattgc cgataggcta tctctggcac accgtttatt
    44581 cagaaaaagg agttgcgaca aggcttttgt cacaactccg ttcataaata tttcctgtca
    44641 atccagcata atcggctgaa taaaatcggc ttgccatctt ttttctttct cagaaaaatc
    44701 aaaataccat atctgagaaa aatactgtcg ttgtgtctta cttcctgaga tcaatcgccc
    44761 atcttttcga tcgactgaat aatcacagca tctgaaatca attcggatgg caaaactatt
    44821 ttcgtggatc tgtcgataat ttcctacttc ctgaatagtt atttttttag gtatgatttc
    44881 ttacaccgtc tgctttgttt ttttgcagaa ctttctgatt ttcggcaaac agcttgtctg
    44941 tataacagct tctggctttt tcaggagttt cttcttccca agcggattgg atcttcatga
    45001 atatcttttg gatttccaga aactgtgctt tcttttcttt agccgttcct gacatgcggg
    45061 acaatatctc cgctttcagc cttttctttt gtcttttatt cttgacgata gaaaaagcag
    45121 aaacccctcc tactgacaaa acagctaatg caagaaatcc cgtttgctca taactagaag
    45181 gacctgtact tggaccataa tacccataag aatctctgcc gtaagaacga ctgccaaaat
    45241 aactgccccc atatgaacga ccgccaaatg aacctctgcc cgaacttcga ccgctagatg
    45301 tacctccacc atgaccaccc gcgacagcga atccttgggt cggcagagca aacaaacata
    45361 ggcccactaa caaaatgatt gctaaataat tgattttttt cattgtcttc ctcgttatct
    45421 tttattacaa aaaactattt ttatcatagc cttgctaaaa ggaagagtca ataaccagat
    45481 gcataaactg tttttttagc aattttgtct tatttagtca ttgagtaaca tcgaacagat
    45541 gtatgtgtcg gcagtccata attcctctat cttcagcatg aacaagcttg cagacaagag
    45601 atctgcgctt cctccaggag agatttggtt ttcctgacaa aagtgcaaca gtgctgtaaa
    45661 tgaacgattt ggtaaagcac tttgactagc agcatgagca agtttttgca tttccactaa
    45721 cgttttctaa tcactgcgat gcaatacgca tgtgtcatct agcgaactca tcaaaactaa
    45781 caacatatgc atttgtttct gtacatatga atcattatct agctccttag ctgcttggat
    45841 tgcaagcctg atatgcggat accctaatat tgcttcttca tatgcaccat ttacgccata
    45901 tttttgcttc accctatgac catgagtgtt ttttttatga cgatattttg tatcaggaaa
    45961 agcagcaagc ttgctgacat catcaagtat tttcgataaa gaaaaaggct gttccatact
    46021 gattttttgt gcaatgacac tgatcaatag ccccattgcc caaatcgctc ctttatgggt
    46081 attgatgcca tttgtagcaa tcagcattgc cttttccgct tttcgcccaa tctcagcaat
    46141 tctttctctt aactcctgat caatagcatg atgccatgct atttgagcca tttcttcaaa
    46201 aaaaggagtt agcgtcttag cagacttaag aaataaagca tagtccatat cggaatgtgc
    46261 acccgcatcc agtgcatcca ctaatccagg ttttggactt aatgttactt ctgcgatcaa
    46321 tgcttcctct accaacgcag cgagttgctg agtcactgct ttttttcgtt cgtcatttat
    46381 catgacactc actccttctt cctactatta cttgctgaaa ccttttttga tagctaccca
    46441 aatgatatga agtaaccagc ttaaagcaaa tgcacaactt gccgtcatta gataagctac
    46501 aagaatcatg actcctgaag gtaactgcag ccaactatct ttaaacaata ataacagtac
    46561 ttggaaaaca aatacattgg ctaaatacgt acgataagca taaacagaca accatttgaa
    46621 caatggcaat tttggcgaat cgtttacaat cattttggaa gcgcctatga agacagcaaa
    46681 gatgatgact aaactgtata aactttggat cgtattcaag taaggcgcat tcgcaaagct
    46741 caagtctcct ggatgtgcga gcaatgaacg gacagaaaag aacgcgataa ctaaggcaac
    46801 aggtaagaaa agataacgta ctctgtccaa atattgataa actttttcat gataaacaaa
    46861 tgcggctacc ccataaatcc cgtaaatcat gaaactagca acaaaccgat ctaacaagta
    46921 ccagctgtca tgataaggcc cagtcaatac ttgtgtttga tagaacagat accatgcgac
    46981 atataaaatc gttgaaccaa tcaatactgg ccatacatat tttttattag caaatacttt
    47041 gtaaccgagc caaacaaaaa acggcatgaa caattggatc tgcagcatca taacggtata
    47101 ccataaatga ggcgctccat cacctgtgac atatttcagt aaaaagcttg cagtatcgta
    47161 tacggagttt gttgttggac tgctggaaat agccaaagat aagccgtcgt ccatagaaga
    47221 tacggaacga ctagttcaaa aaacttttct ttcaaaaatt cccaataaga caaatgctgg
    47281 cttgttttta ccatattata aacaattgca aaaataaaca ttggtgctgt gtatttaacg
    47341 atgacgtaaa aggtaccaat aaaagctgta tttgttgcgc tgtggctgtt tgccaaagca
    47401 aagctcaata tcgtttgcat cataacagcc tttacagctg atactttctg gaagtcactt
    47461 acgttgattt tgattttttt gttttcaaac atctctttct ctctcttttt ctttattacc
    47521 aaagttcttt agttaattta ggtccttcga ctgttttcaa tagaattttt ttctctggct
    47581 ctctcaaata ctcttttagt gaaaaaccgc cttgccgagt cactacttgg gtatcgaatt
    47641 gctcaaaaaa agcaggatgc gattgaatta cctgaattgg caattcaatc ggtgaatttg
    47701 cgtacagtag caaatcaaaa tcactggttt cttttactgc cggaatccca gtcgataatt
    47761 caaatcctaa gctgccgccg attccccatt tacatcctga taatatgttt ctagcagcat
    47821 gaaattgctg ataaacagga taatccatga ttttctcttg tctatacgat tcttgttcga
    47881 tgatgtccca cggatgaatg acttgtttga cggcatgttt tgagatttct gttgcccatc
    47941 tctgattgcg ggcaggtcca cgtaaaccaa tcggtagtat ttctgcgtga tccgtcgacg
    48001 cacgacgaac tgttccgaac atcttttcag ttagaacgac ccattcaggc aaggtgaacc
    48061 cttctatttc atgtttatca aaaaggacaa tatcatgtgc ttgaatcatt ttttatgcat
    48121 cccattcttc gtccattttg gcacggactt tatttgtttc gacacgtccg aatgttttgg
    48181 ctgtttcgtt tgtgtatcga tttgtcagca tcgtgtcatt tgtattgcgg acatcttgaa
    48241 tggcttcatt gattgcttcg atgactttgc tgacattttg ttctgaagca gcttggtcgg
    48301 tgatatcttc taggaaatga tagatcgctc ctagttttgt ataattattg atatcgtaag
    48361 cgatagctgg aactttttca gcagctgctt cgatttcagc aaccgaacgt tttgtcacac
    48421 gtgcagcact ttctttagac atcgcttgga cggtaatttc tttattgttt aaagcaatca
    48481 agcggcttga ttgtaatcca tgtgctaaaa atgcacctga aatagcattt cctgcaatca
    48541 atccaataac tggatatcca gcttgacgga attttgcata agcagcagca ctattagcca
    48601 aagccacatg gataccgatt aattcttcgt tatagccgta tgcttggctt ggtacatcga
    48661 tcacaacgat gattggccgt ttcacttttt tatctttatc ttcttcgtaa acgtgatcca
    48721 aaatttgtcc catgcggaaa ccttcaacta aaccaacttc accgtttcgt acacggtaca
    48781 tcgggttttt aggatcaggt acgattgata tcacgatggt ttcttctatg aatttacttg
    48841 atttaccata atacacagta ctaatagaag aagttggatt tttcatgcca gttagtgccg
    48901 caaaccaatc gtaaccaaca cttgctgcag ctggttcatt tccttctgct acttctggta
    48961 agtcaattga ttttgcttga tggttagcaa ttgcttcatt gtaatcgtca atggccattg
    49021 gttttgaaag atctaaagaa tcaagtaatg ataaataaaa tgctgagctt tcactgcgat
    49081 gcgtgtcttt tcgttcgtcc attgctgcga tgacagcagc tttgattgct tctacactat
    49141 cagtcactaa ttcatcaatg atttttgact ctacccgttg acgagatcct aatgtattcc
    49201 aaataaagtc tttgtcacta gaatcaaatt ctcgaacgcc tgcttcttgt tcaataactt
    49261 ctggtccatt caaaccgact ctcgcttttt ttgttgcgat taaataactc attaatgctg
    49321 aagtaatcga cataccgcca aatgatccta cacgtcctgg aatcaaaccg atgactggta
    49381 catattgttt tagagcaatg attacatttc cgatttctga aatagataac agtccgtagt
    49441 ttgcttcttg caaacgaaca ccacctgtat ctagtacaat gactggaaag atctcatttc
    49501 cttttttatt ttcttctaac acatgttcaa gtgcagcaac gatttttgca ccggaaactt
    49561 caccgatacc gccaccttgg aattttcctt ccatagcgat tactactgct tgtttgccat
    49621 tgatcgtgcc acggctgaca acgatcccgt catcactttc agggacgatt ccttgtgctg
    49681 gcaaacttgg cgcgatgatt tgatcgaatg gatcaatcaa ttcacgtcca ttgtcaagta
    49741 gagcgttcac tcgttctctg gcgtttaatt ctacaaaact atttttcatc tgaaagttcc
    49801 tccattgctt gcgtcaatcg taggtacaca actcctggtg tagcaccgaa gtcattgatc
    49861 acgatatctg ctgaaattgg ataacgatca aaaaatctcg ttaatacatt gttccatact
    49921 tcgccgaagc catcgcttcc tgtacaaatt tttacagtag attctgtttt ttctgttgga
    49981 aacatcaaga tttccaaatc tcctgaaccg accactccta catggatttt cttattgatc
    50041 ggctgtttcg ttggatattt ataagctaat tgttccatgg atctctcccc tttttgtaaa
    50101 acagagcata cacgttctgc ttattcgtct ttcttcttcc atctgggtaa atggcgacaa
    50161 aaggtttttc tcgccaattt tatgaccaag ttctgaattt cattggtggt tcatacaggc
    50221 catctgacca ttcgatcaaa tcatcgattg tttgtgcagc taataatgaa cgtttcgctt
    50281 cactgcgttt gatacctaga tcttctggta atttcacgat gccggcttca cgcaagctat
    50341 ctagtgattc tttcgtgcta gtcattccaa gaggtgtaac accgccaatt gctgcaattg
    50401 ctgcttgacg ttcttctttt gtttttgctt tgtacaaata agcaattcct tcttctgtca
    50461 cgatatgggt agtatcttcg ctatagatca tcactggtgc attggctaag cctgcctgtt
    50521 tttgaacaac taatgcatct aattcttcga caaagactgg ttttttattt gaaccatatg
    50581 tttcaaccat ttgaacaact aatttttgtc ctttacctaa tggatcatca ttgtcgcgaa
    50641 gtgattgcca tgctggtgta gaatgacgac gaccgccagg attatgtccc atgttaggcg
    50701 ctccaccaaa accagataaa cgtccttttg ttacggtaga agaatttcct tcgtaatcta
    50761 attgcaaggt tgaaccgata aacatatcga ttgcatattg tccagctgct tgtgataatg
    50821 ttcggttact gcgcatcgtg ccgtctttac cgacgaaaaa gacatctggg cgagcagcaa
    50881 tatatttttc cataccgact tcaccaccga aactatggat actttcaacc caacctgatt
    50941 cgattgctgg aatcatcgtt ggatgtgggt tcaataccca gttcttcgcg attttccctt
    51001 ttaaccctag tgattcacca taagttggca gtaataattc gatagctgct gtattgaatc
    51061 caataccgtg attcaaagat tgtacgccgt gctctgcgta gattcctttg attgccatca
    51121 ttgccattag gatttgcaat tctgtgatgt tttgaggatc tcgtgtgaat aaagcttcta
    51181 attgataagg ttcatctgct tttacgacaa cgtctaccca gtcaccaggg atatctacac
    51241 gtggtaaaga atcgacaact tcattgactt gaacgatcac gatgccatct ttgaaagctg
    51301 cagcttcgac gattgttggt gtttcttccg tattgaaacc tgtatacaag ttgccgtctt
    51361 tatctgcttt atctgcaact actaagacaa cgtttggtat caaatcgatg aataagcgtc
    51421 cataaagttc caaataagta tggatatcac ctaatttcat tgtgccgtct tcaatcattt
    51481 gggcgacacg caagctttgt gcacctgcat aagagaaatc gatcttgctt gcgattcctt
    51541 tttcaaaaat attcaaatgt tctggtcgtg agatactaga catgatcaaa tgcagatcat
    51601 tcactttttc tgcatctact ttttcaagac tttctgaaag aaaacttgct tgtttttgat
    51661 tgtcgccttc taatacaact ttgtcgccgg gagcaatcag tttttccatt acttctacga
    51721 ttttttcagt ttctacatac ttgccattga gcaagttgct gattttttcc aatcgctgcg
    51781 cttttttttc gcgtttagaa gcccagttgc ggtttggttt ttccataatt agtcatctcc
    51841 atttttgata tagaaacgtg gcgtcatcaa taccatgttg gtctagattg atcgtacgga
    51901 tccctttatc tacaccaaat tttgtatgaa tcagctttgt caaagtattg ccggggggaa
    51961 attcaatcgt cagatccatt cctaattctt ttgccaagtc catcatttgg ttccactgca
    52021 cagggaaagc tagattttcc aacaaatctt tacgaatctc ttctgcgtcc tgagtggctc
    52081 ttccatccgt attttttata tacaaacatc tggcatcaga tagatggaca gactgcatat
    52141 acggcttgaa tttcttgacc gtatcattca tcaagagaca gtgagatggc acagggactt
    52201 tcagcagctt cgctgtttgc gcttggtgcc gtttggcttc tgccaacgta acggataaac
    52261 cttcgatact cccagataac gtatgttgca tcggacaatt ttcatttgac agataaactg
    52321 gatggtcagg atcaaatgtt ttggctacca tttcctctgc ttcgcttctc gtcacaccta
    52381 ctactacgcc cattgcataa ccgcttggat atgcttcttt catcaagact gcgcggtgat
    52441 agactagttc cgctgcattc tcaaatgtca aagatcgaca agcaacagct gcacaaaaag
    52501 caccgatcga atgtcctgct gcaatatcag gctgaatacc atttttttcc atttcatcta
    52561 aataaaatac tgcttttgtc agtaatgcta gctggataaa aaccgtatct tgatagtttt
    52621 cttctttatc gactaaccga taccccgtca cattttctaa acgttccatg tacatttcag
    52681 ggatatccgc taacatccct aacttctgac tgccttgtcc aggaaataag aaacctgtac
    52741 tcatgagcct cgcctcctca gcacttactt tttccactgc tatcttagtc aatttctgct
    52801 ataataaaaa atgccaaaaa aagaaaaaac cataacaaaa aggtatggat gggatggata
    52861 attatgaaat tgcttcatct gcagtatttt aaaaaagtat tcgaaacaaa aaatgttacc
    52921 caagcagcca aagaattatt tatttctcaa cctgccttga gccgtgcgat caaacatttg
    52981 gaaaacgaat taggtgtccc tttgttttac catagcggca gaaacattga gacgaccgtt
    53041 tatgcagaag aattttatcc atatgcagtc aagacattat acacattaga agaaggaatc
    53101 aattattgtc ttcggtcaat gagaagatcg ttacttccgt catattgtat ttagaggtag
    53161 cttctgtttc tattccgaac ttagtccgaa tttttatgga aaagcatcct gatatccaat
    53221 taacgatcat gcaacatcat ttaccagatg atattaccga tcctgtactg tacatcactt
    53281 cggagatgaa gcctggcatg accaatattc ctatcatgaa agaacctgtt tatgtcgcca
    53341 ttcctaagaa acatcctctt gcgaaaaaag aacatctgca attagaagat atccagcgca
    53401 cccccatctt gatgctgtca aagagcaacg ccttccgtca tacactggat gcagcacttg
    53461 aagcgaaaag tatcgaattg acgatcagtt cgacgacaga tgatcctgcg acattacgtt
    53521 ctattgtcaa gcaaggatta gctgttagct ttttcccaaa agtttcttgg tcttacgata
    53581 aaaatgatcc tttcgtcttg cgggaaatca aagatttgcc cttgacacga acaatttatt
    53641 tatcttctag cttcacagaa gatcatcctt tgatcaaaat gattgcaaaa acgatgcggg
    53701 aattttattt aggaaaataa aaaaaaaaga gctgggacaa aattcttgtc gcagctcttt
    53761 tttcgaacaa acggtgttcc agaagtaatt tcttagaaaa taagccgaaa tttcccaaaa
    53821 atttgagaag caattttcgt aaattccttc ttatttcttg aagctaaaca tttctgtcac
    53881 aacctcttct ctattgtcct agacattcat tgcgttgatt taaaacttcc atcaatcaaa
    53941 tctgctagtt catctagctt ttctatttta cgtagatcaa tagagatcca atgttcttta
    54001 ttcatgtgat aggctggaaa atacccctct tttcctcgca aaattccgcc aagttcagga
    54061 tcgaccttca aatcaagaat attgatgatc ctatggtcac ttgcatcatc atagattttt
    54121 tcttctggaa cagacatcag gaccccaaac cattttttgc ttttttgatg tctgaatacg
    54181 caataatcag gaaatttttt aaaaatatat tctggagaca aattgtatgt ttcattgatg
    54241 tgatcgatta gttctttttt cgttagcatc tgcttccatc tctttcttat tgattcgctt
    54301 tattcaaaga taccacgtaa gaatagaaaa atgaatacaa gtagctgcta atcaaatact
    54361 tcgatatttt tcttttttat caactcttaa atgttcaatc cgcctgtgtt ggtcttgcta
    54421 tcatctacta ttaaaatgtt gattattgtt tgcccaccta ttttttgatt ttagtggtat
    54481 catttttcaa gttcatgatg aaattcttat catcagtaaa tgctccagaa aaataaacaa
    54541 tgattgaaaa atcagttgaa acttgaccac cgtttatttt ctccgtattc ttagaataga
    54601 agggagtctg attgatgtat tcactaatga aaaaaatcat taccgaaaaa gatattcgac
    54661 gtcatgtttc tttagtagaa caattgctga atcatacaaa gataaccgta aatgaattag
    54721 ctgaaatcat agggacaacc gaacgaacga tttattctga ccttcaatct attcgctctc
    54781 aactgcctga aggctgggac atcattagcg atcaagcagg gattagtttg caaaaccagc
    54841 aaaatttatt agcaaatgac ctttgggaga tttttttcaa acaatcagtc agtgtcgaac
    54901 tattgaaaag tctgcttttt acaaaaaaag ttgctgtccc tgattttcta gccgaccatg
    54961 ggctttctta tgggacattg aaaagacatg tgactaaaat caatcagcgt ttagcaagtt
    55021 acgacttgca gatcgatttg acgaaatata ccgcttctat tcttgggaaa gagcgtgcga
    55081 ttcgcacttt ctatcatcga ctgctgattc cttttactca taacaactat tttttcgaag
    55141 attattcgat tcatgaatcc cattattttc aatttttaaa aacaataaat cgaacggagc
    55201 tagcggttga aacagaagag atttttggaa catgttggtt ctttatcaat acgatccgta
    55261 taaaagcgaa ttgccgatta gattcttcta tccatatcaa cagtacactc tcctccctgt
    55321 atgactctgc attgaaaaaa ttgtatttaa cggaaggtat ttatctcaaa gacactgagc
    55381 tttcttttgc ttctttctgc tttctggaaa gttggaatta caacaataac tatggacaag
    55441 aaaccgcttg ctgcctgcat cattctccat ttttcgaacg cctcgaaacg tttgtagagg
    55501 aactagcttc agagctgtct cttgatcaac taaaaaaaca tctctaactg ataacttggc
    55561 tcttctcgta ttaaaatatc atgaatcgcc gatattgatt gaagcattgg accgtcaata
    55621 ccattttttt tagaaacata tgatcagcaa tatgcacatt tgtctcaaca gaaagagaat
    55681 ttactgaacg atctaaaaaa agaaatggaa atcaatgaac cgaatttctt ccttcaactc
    55741 ttatctttac tgatccaaca aacgattttt tctatcaaac cgaatttgtt caatgtctat
    55801 tttattttcc aaggagaacc ttcatggaag gcattctttc agcaggaatt aaaagattac
    55861 ctgggaaaac gtgtctgctt cttacctatc gaactaaccg atctttccac tatttctttc
    55921 gaaaaaacag atatcttgat ttccaatttc ccgctggatt ctctagatat ccctatcgta
    55981 tacatttctt ctattcctac gaaaaatgag ctcaatcgtt tgactgaact cactttcaaa
    56041 acttttttat agtaacggct agcgactgta ttctctagct gttttttctt ttttgcgaaa
    56101 cagtttattc atgatccctt tttctatttc tacaactaag aatagaagaa tcacatttgc
    56161 tccaatcaat aactgttgtg tcatcgttaa acttactgta ccgatcagct gctggccgac
    56221 tggtagaaaa ataaccatcc cttgcaataa agcaaggata cctaatgaaa gaaaaagcgc
    56281 tttgttttgc aaaagaccct ggttcaatga cggcttggac aactctctgc aattgatcat
    56341 gtaaacagct tgagctaaga cgatattttg tagaagaatg gtttgctgta agctttgccc
    56401 ttcaaactgc atagccaata aataggcagg aaccatgatc agtagggaga catagatgat
    56461 ccgaaacaaa tgataaccag ataggattcc ttgctttgga tctcttggtg ggcgcttcat
    56521 gatttctgga tctggcgact cgaaccctag tgcataagat aaagtgattg tcgttaccat
    56581 attgacccat aaaatctgta ctgggaacaa aggtagcggg cgattcagca taagcgccca
    56641 aatcaagatc aatccttgag ctaatgcagt tggcaagaag aaattgatgg ttttttgcaa
    56701 attatccaaa atccgccggc cctcttttac ggcttttgca atcgtatgga aattatcatc
    56761 tcctaaaacc atatcagctg cctgtttgct tacttcacta cctttgattc tcatagcaat
    56821 tcctacatcg gatcttttca aagcaggtgc atcatttacg ccgtctccgg tcattccaac
    56881 gatttatcca tttttctgca aagctttaac aattcgcagt ttatgttctg gcgtcgttcg
    56941 agcaaagaca tcgactattg gcacttgctg aatcaactct tcatcagaca taagatccaa
    57001 atcgatctct tctaaaactt tggcagtatg ttttaagcca atttgttccg caatcgcttt
    57061 tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag atccctgctt gttgacattc
    57121 ctttacagct ttgactgcac tttcttttgg cggatcgatc aatccggcga tccctgcaaa
    57181 agttaattgg gttaaagtct catgcgtgag ttcctgtttc gaatctactt ttttataagc
    57241 aaatcctaaa acacgctgac cttttctcgc aaattctgca gctgtctgac tccatgcttc
    57301 ttgttctgca gaagaaagag ttgatttagc aaataatact tctgatgcac ctttgacgta
    57361 aatcaccgct cctttttcct ccgcatgcat cgttgccata tatttgtatt cagaactgaa
    57421 cggaatcttt gcttctattt ttttcaaagg cagattggct gtttctgtgt gtcgaatcag
    57481 cgcaacttct gtcggattac cacttaaatt ttctgttttc tgttcttctt ttgtttttaa
    57541 ttcctgacag tttttcataa tggacaacac gcaggcttct tcttttgcag ctacatccac
    57601 aactgtcatt tcattttttg tcaatgttcc tgtcttatct gaacagatca ctgtcataga
    57661 acctaatgtt tctaccgaag gaagtccttt gatgatcgcc ttttcttttg ccatttcatg
    57721 tactccaacg gataggatca tggtcaaaac agctggcaat ccttcgggta ccattgacac
    57781 caccaaagca atcatagccg aaagaaggat atgccaatcc ataccatatc gaaatgtcgt
    57841 aaaaataact aagaataggc tcaaacaaag aatgccttga aaaatttgtt tgtttaatcg
    57901 gttgattttc ttaatcagag gcgtcgtttg ttgttcgacc tattgcaatg cttgattgat
    57961 tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca ccgactgcag aaccagattg
    58021 tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg atcgattctt cttctgtcaa
    58081 aacggcatga cttttttcag atgcttctga ttctcccgtc aaaatcgatt cttccactaa
    58141 taaatcatgt gcttcgatta atcggatatc tgctggaacg acatctccgg cacgtaagac
    58201 aatgacatct ccttttacca gttctttcgc agggattttt tcttgcatcc cgtttgatag
    58261 tactatcgct tcttgtccca tcagctgctt caaaccattc aatgattctt ccgcttttct
    58321 ttcttgccag taactaacga gtccattgac gatcactacg aataaaataa tacttccttc
    58381 aatatattca ctagaagcaa atttcaaaaa agaggcaatg atcaacacga tcattagcag
    58441 atcagtaaag tgctttgcta tttttttcca tcgttttgtc tgctgccttg cttttatttc
    58501 attatatcct tgatcagtca ggcgtttctg tcgttcttct tgcgacaagc cttctgttga
    58561 tgtttgatag tttttcaata catcttctac cgtcaatctg taccagttca tcctatcccc
    58621 tcctgtttaa ttgatactgt tattatacgc agggaagttt tttcaggcgt atcgattatt
    58681 tcaggggggt ctgaaataat tcgttttgca gaatgtatct gggcctaact gatatttcta
    58741 ttgcttattc ttttatatac aaaatttaag aatacaaacc agataaccaa taaaaaagga
    58801 ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa ttgaagtaaa aaacaccaac
    58861 aaagcaagat ctttatatct ttgtgtagtg ttccgtttct tttctttaat aaattgaaca
    58921 tgtgtacaca agatcgaaga ccacacaaat agaaaacaac tcgttgtcat gcctgccaaa
    58981 agctgaaaaa acaatcgact atcagctaca aaaaaatgta gacaaagacc ggcaaaaaat
    59041 aacattcctg taattaaaat accgtgtaaa ggtacgcctt tcttagaaag cgcacaaaac
    59101 caatggggca aacttttctc ttttgctgtt tgatataaaa tcctgctagt actgtagact
    59161 gcactatttg ctgaggacaa agaagcaacc aaaatcatta gattgacaat tgctgctccg
    59221 ccttggcaat ggatcatttg cagcatagtc actaaaggac tttcttttaa atctaaacaa
    59281 tgccatggaa caacaaataa aatcgacaaa atcgttccta tatagaaaaa gagaatacgc
    59341 caaggcagac agttgattgc ttttttcaaa ttcttttgcg gatcacttgt ctcgccaacg
    59401 gttaacccga tcatttcaat tccagcaaaa ctgaataaaa ccataggaaa tgagactacc
    59461 attccagcaa agccaaacgg tagtaattca tcgatctgtc caaaattttc tttgattccg
    59521 atagactggg tatgaaaatc tgagacactc aatcctatac ccaataagat aaaaaggaaa
    59581 atccccagga tcttgatgat tgagaaaaaa gattccaatt caccaaacca cttgactgca
    59641 acaaggttga ctgccattaa gatcattagt gcgctacctc cgcttatcaa agctggtaca
    59701 tgtacaaacc agaaatggat atacaatcct attgctgcta attccgcaag tccagctcca
    59761 agccagcaaa gccaataagt ccaattgaca aaaaatgccc actttttgcc taaataatag
    59821 tcaacgaaat cactgataga gttaaatgta ctatttgcta aaaataaatc acccatggcc
    59881 cgcatcaaac tataaatagc caaaccgcat atcaaataaa cgagaataac agctggccct
    59941 gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg ttccaattgc gccacctaat
    60001 gcgattaact ggatatttcg attagttaga gtccgtttca ttgccggttt ttccatcaag
    60061 ttcacctttt tctttttcaa gcagcatggc gacttcctgt tcccaagaag gtttctttat
    60121 tttttcaata cagagtggta ctacaaacaa aatgacactg atggctgtga ctgcaattag
    60181 attgatccat gttccagctt tcatcgtcag tccgacagaa aatccgattg ccgcaaataa
    60241 aacaataacg acagtccaaa ctccaagatt gctctttttc tctttttttc caattctaaa
    60301 tggtcgattc aactttgcat gtgtataacg caatctcaaa actgcgatcc ccatgatgac
    60361 atagacgata cagtagataa cagtagttga atttacgagc atcaaaaatg cttgattgac
    60421 gccaggaatc agcaaataaa aaagtgcaaa gattgagata atgacagact gagtcaaaat
    60481 aacggatttg gatacgtcaa attgattcgt cttccaaaaa ttgaattttg gtggatacgc
    60541 accttttcta gcactttctg tcactgtctt tgctggtcct gaggcccaag cagataattg
    60601 gactgctaca ccaatgaaga caagtaaact aaacagattc acaataagat gtggcaaacc
    60661 aagaatttgg caataaatcg aaatagattg cgcgatatta ttcaattcca tttgtccttt
    60721 tggtacgaca tttgctacaa ctaaagcatt caaaatattg acgaaaaata taaaaattaa
    60781 cgcggcaaat actccgcgaa tatacgtttt aactggattt ttcaatcgag tgatatacac
    60841 agaggacatt tcgataccag taaaaataaa catgatcggc gcaaaataaa caaatgagga
    60901 tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa ctgcctaatg ttccggttgg
    60961 tacgatccca actttgatcc acgcagcaaa ccctaataag agcatcatcg tcaaaggcac
    61021 gtacaatcct aaccaaacac cgacttttcc gccgattttt gccatatcaa atttcaaatt
    61081 caaaaaagtg atgatccaat aaacgactaa aataacaact aatgtaaatt tgctgttcaa
    61141 accaagcggc acattatcga taatattacc aaacaacgga gcaagtgctg aagcgaccat
    61201 caccatccca ggaaacattt ggacccataa cagccatgag acaacgaacc cccatttttt
    61261 tcctagagca ttcgataccc aaagttctgg accacctttt ttgggaaaca ttccggcaaa
    61321 ttcaccagag atcaatgaga ttggaaaagc gtacaataat actgcaacca gcatgtaaaa
    61381 aatcatcgtc cagctcgttg ctgctacatc tggaatattt cgtacacttg ctacaagggc
    61441 gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt gatgaaccag tatctttcat
    61501 ctttattcct tctttcaata gcgacatatg tttttgtttt cttttatttt tgttcaagaa
    61561 cagaaatgac aaggtttcat cgattcagta caaaacccag tatcaccgaa acatccattg
    61621 aacgatctta gtgaactggg tttgatacat gaaacgaacg atccaactta tagaacgttt
    61681 tataaaaaag aatatcttgg cgaggaatat atgaaaaaca gggggaaata caattgtttt
    61741 gatgaagcaa ataattgtct gttctccata tgtctcttgc tattctttca cacaaaaatc
    61801 agttctattg gatgatttcg tttagtttct tttccattgc tttggcgatt tcttttgtat
    61861 aaaaattgaa aattcgatca tcattcaaat aaggagtcat gatagccgca cgtagcagag
    61921 tgattttctg ttcttttttc cattcttctt tcgttagacc cattctttca acaaaacgaa
    61981 tcggagaatc accgtaatct tcttgtgtaa atgtagtatg agaagtaata aaccgttcac
    62041 cataaacatc gccatccata taagaagacc gatcgaacat tttttcattt aattcattga
    62101 tagcgttcaa atctgtacag ccttgttctt tgaataccca attgaccata ttaaaatcag
    62161 gatgattcaa tggatagact tcgattgttt ttcctttaac agtaaaagtc atatgatcga
    62221 gaaattctct gaatctttga gctgcctcga ttgaagcccc aatcaattga ccgtatccgg
    62281 tcacattcaa tggcaataca cgatgtgttg tccagactgc ggcggcggtt gctccagctt
    62341 ttgatccttc caaaatataa gctcctaaca tatctggagc ctttactgat ttttcaaaca
    62401 catatggcgc gaaataagaa atgatatttc tcatattttt atgtttgatt acgattcctc
    62461 cagcagcata agggacatag cccatcttat gaggatctat cgtgacagaa tccgcttctg
    62521 aaatagctct gaacccttca tatacctctt tgtcaatcgt tacgcagtgg tggaaaacat
    62581 gatgttcttc aaagaattca gctaaggaag catacggcac aaattctcct gcttcgttta
    62641 gaaacagtga acgcgcatag ccgccatatg cggcatcaac atgtaaatag aaatagatac
    62701 cttcatcttt taacctttct cttaattgga cgattttatc tacactgtct acttgacctt
    62761 cttctgtcgt tcctacgaca gcaaccacac ctagaattgg gattttttgt tcagctaatt
    62821 cacgaattgt tttttccaaa gcattaatat ccattcgata atcctcttga actgggatag
    62881 caaccatctg atctaagccg actccgcaaa tatctaacgc cttcatccaa gaatagtgtt
    62941 ttgtttgagg gaccagccat ttccctaatc tttggatatt ttttccgcta cgggaagaag
    63001 ctgctttgac ctcatccatt tcttcgtccg taaagctttc ggtcatttcc aaaatttctt
    63061 caacagataa attcaaaagt tcccactcag acattttttt cactttttca ggcagcactt
    63121 ccttgacagc taaaggaatc gatttgatgc agcgggcgta ccataatcct tcaagattag
    63181 caatagaacc atctgctgca atgtgtcccc atccgtctgt catattgaat aatgaagcaa
    63241 aatcctgtcc tacttctgct tccatttgcg aagttgccat agaagattcc aatgcaacat
    63301 tatttgggtt ccacagcatc gcatagttgt aagctaataa agcaggcatc aatgtttcag
    63361 cattcatttg tccccagtaa cggcctgctg aatgccatgg gatcgatcct gcgcgtaatc
    63421 gctgactgac ttcatccaag acctctatca tatggtcgcg ggtggctaga taatctggag
    63481 tgagcttatc tccttcgcta attgcaggca tatctgaagg aatataattt tctctccatc
    63541 caagatgttc atctaccatc ttgttcaaca acattttata aactggtcca ttttcagctt
    63601 tgtcgcctat aaagacggcc ttgatatcca tatctttcat ttttcttccc tccatatttt
    63661 gttggtcaat gtctcgcaga tacatgtgtt tatctgtaac acctcttcac tatagcgcca
    63721 aaaatgagaa atttttttta taacatcaaa tccacgtaga tttttacaaa caaaaataaa
    63781 taaagtcgga tttttctgat aattaaacgc aaaaaaacga acattttttc aataaatcag
    63841 tgttctaaaa tagataacct aaaaaaagcg aacaaacaaa taaaatgact gtccttcatt
    63901 cactaaacat atttttattt ttttatattg ttaaaaattc acaatttatt ttttaataga
    63961 attatctgtt ttccaaattt tataacataa aaaacctttt ttaacggcat ttatatgctt
    64021 tttcagaaat taactgatga tttcaaaaag aaaacatgtc ataatatttc ccatattgtg
    64081 caaaaaaata caaggcggag gctctactat gattgaagaa tatattgaga aaaatatttt
    64141 aagacagctg tttttatgtg ggcaattcta tgtaaataaa gaagtcaatc tagaaaagct
    64201 ctccaatctc ctgcatgttt gcaaaaccac tttgctcaat gatatcaata acataaaaaa
    64261 agaatttgaa gaacagatcg cttatacaca tcgggaaaag gaccgttaca ctttatattt
    64321 ttcagaacat atacctcgat gcaagatcat gcagcaagtg tctcaaaatt cgttgttttt
    64381 aaaaacttgt ctgctttatt tggaagaaga tgaacctgat tatcttcaat taactgaatg
    64441 tgaattcatc tctgtcagca aagcttattc attaaaaaaa caagtattag cttattttaa
    64501 tgactgcggg atcgaaatcg accgctactc ccctcgactc actgaaatgg aaagacgtct
    64561 gctgcttctt aatgtgtcgt atcgtttagg cggttttaat tcttgggagc tacctgaatc
    64621 gttttttgaa cgtaccgatc gtttcattga atccgtaacg gaaaatagtg gcagatttta
    64681 tgataaagaa aataaagaaa tcttatcgat tggttttgct atcagtttcc tacgccagca
    64741 agtgtgtgct gtgacaatag attcaaaatt tatcgaagaa atcaaaaaac gtcctattta
    64801 caattatgtc gaatcagcat gggaaaacac tgatttccaa acctattata aaaaagaaga
    64861 attcgccttt attttgacac tattcaatct ttgtaattat ggtttccatt cttatcagtt
    64921 gattgcggaa gactttcaac agctacacca agtgtttatt gacaatactc ccgaaataaa
    64981 agaactagtt gctacctttg aatctcactt caatcaagaa ttattcggaa accaaccgtt
    65041 cgaacgtgcg ctgattcatc tgatgcgttc ggcttgggat aactatcaat tattcatgcc
    65101 agaaaaattt tatcttttaa atgaggaaca gacaaattta tataaagaag tccagacaat
    65161 tttttccagc tggtccagcc agttacctta tgatctacgg ttgaatccta attgcatgcg
    65221 ggcttttgtg atcgaattga gcggaattct ccgtctgaca aaagaacatc tgactatcta
    65281 tatcgtcaca aattcggatg ttcattatct gatttatcgg gaagccttgg aagctgtaac
    65341 tactttcgat tttcaagttg caccaacaat ttattcaagt atatctgata taaagaagta
    65401 tgcgcagcag tcctcaaacc gggtattatg tgaacgtaca ttatatacac cagatgcggt
    65461 ccaatatgaa aatattattc ctatttcgat caatacgatc gatcgagcta tcatttcagc
    65521 tgtgcaaaac aaataagggg ctgtgacaaa aatcatgtca cagcctctta tttgttgaag
    65581 atgcccattt ctgtaacaat ctcttgatac acgtcaggct cacaacctca cttattttgg
    65641 acatctacgg gtccgatctc tttatttaaa aaataagaaa tgatggttcg gataactacc
    65701 aacgccgcta gttggaaaat atcctgcaag gtaggtttga taatcgattc gatgatccca
    65761 gctgaaacaa gaatttccaa acttagaagt atatagcctc ccaacatctt tttaatagca
    65821 ttgttctcgc tgactcggtc gttttgcact tttgtatgga caaataaata tttcaaaaaa
    65881 tccttgattg atagacaaac accccaaacc aataaacaga tagcaaagat atcaagtccc
    65941 agtacaagtt tttcaaaata tggaagaaga aactgcatca actctgtcgt catcctacct
    66001 ctcctcttct ttgtttttct ttgtcttcta tttcctgatt caagaaataa gaaatgactg
    66061 ttcgaatgat aataataagt gcaagcttca aaatatctgt ccaagtcggc ttgataactg
    66121 actcgatgat atcagcaacg actagtactt ccaagcttaa caaaatataa cttgctagca
    66181 gttttcttgt atctgcatta cgagtattac gttctgcata gtttttcttg acatgagacc
    66241 aatcaaaaag tttgaataga acaagaacga tcccgacaat taagataccg atagaacaaa
    66301 tatttaaaat agcaatgatc cattctagaa attcaattaa atttgagcga aacatatacg
    66361 ctacctactt tcttttaatt tgttcctatt cttttattgt aaaagaaagt taccttgttc
    66421 gtctatttaa aagttgttct aatgataacg gttcgcttaa atttttcttt ttcactctcc
    66481 catttttctc ccctcatcca taaatcgtgg aagtatccaa aacaacgtag caagttaaaa
    66541 taaggcttag tccgtcatca gcttttcaaa gaagcactgt ctcggtgctt ttttgtaccc
    66601 aaacttctaa aaatagaacc atacacctcc tcaaatcttt tcttcttacc taccctattt
    66661 ttatttacag gaaaatcaga cacttcttct tttttgtcaa aatttccttt ttcttctcgt
    66721 cctatcattt ggcattctaa taaaaacgaa ctatccttgg atatagaaag agaaaacaag
    66781 gaggcgtttt tcatgagtaa aaaattaatc aaagtcggca tcggtttagg tttgcttgcc
    66841 cttggcgctg catatctagg aaaaaagaca ggactattcg aagatgacag tcatttatat
    66901 gatgaattcg aatcaatcta gcatcctatt tcgttgaatc actacggaca tacttgaaag
    66961 acattggagg aatcacaatg agaaaaagta aatcaataat gattggtgcc ggtattgcca
    67021 atatggcagc ggctgtttat ttgattcaag aaggcaaatg gaaaggggat cagatcacct
    67081 tttattcatt ggatgatcac ggttcaaatg atggtgcacc gaccgacact gtagtggatg
    67141 aatattggaa taaaaaccat ccattagaaa accaaaaagg ctatattgct cgaggcggtc
    67201 gcatgcttaa ttaccgtacc tatgtagatt tgatggatct gttaagtcga ataccttctg
    67261 ttactgagcc agggatgact gctgaagaag atacgagaga ttttgatgca aaacaccgaa
    67321 cctttgacaa agcacgtttg atggaaggtg gcaaaggaat cattgatggc ggaaaactgg
    67381 gattcaataa taaagaccgt attcttctga ctaaactgat tgctatgcct gattcagaag
    67441 aagaaaaact agacaatatc actatcgaag aatatttcaa agatgatcca cacttttttg
    67501 aaacaaactt ctggtttatg tgggaaacaa catttgcttt cagaacacgt agttctgctc
    67561 aagaattgcg tcgctatatg catcaaatga tttatgaatt cactcaaatc gaacatctag
    67621 tgggcgtcaa tcgaacacgt tacaatcaat acgaaagtat tatgcttcct ttgatcgagt
    67681 acttgaaaga acaaggatgc aaaatcattt tgaaccgccg tgtcactgac tgggaattca
    67741 aagaaacacc gatgcaagat gaaatcactg tgactggttt gaaaatgacc aatacactga
    67801 ctcaagaaga agaacacgtt gtggtcgatg aagatacagc agttattttc actaatgggt
    67861 ccattacaga ttcagctact ttaggagatt acgaaacacc agctgtagaa aatatggatt
    67921 atggtgcagc ttctagtctc tggaaaaaag ccagtgaacg tttctacaac ttgggaaatc
    67981 ctgacaaatt ctttgctgat cgagatgcaa gcgaatgggt cagctttacc ctgacgacga
    68041 aaaaccattt gttgttgaat gaaatcaccc gtatcacgac tcaagaacca ggaaatgcct
    68101 tgaattcatt tatttcaacc acaccgatca cgccgttagg ccaaaaagat gtcaatatgt
    68161 ctatcgtcgt gcatcatcaa ccgcatttta cgtctcagaa atcaaatgaa acagttattt
    68221 ggggttattt cctttatcca agacgacgtg gcgaatttgt ggataaacag tatattaaaa
    68281 tgaatggaaa agaaatgcta gaagaactga tcggacaact ttcaaaagta gatcctggac
    68341 cagtgaatat tcgtgaaaaa gaaacagaaa ttttcgacag cgtgataaat aatatccctg
    68401 tctatatgcc ttatgcttcc gcgttgttca ataatcgtgc caaaagcgat cggccaaaag
    68461 tcattcctaa acattcgacc aatctggcct ttacaggaga atttgtcgag cagccttatc
    68521 agatgatctt tactgaacaa agtgctgtac gttctggaga aatcgctgct ttccattttg
    68581 cgggtatccc tatgtcccgt cttgtcaaaa caccgcgtta cgacaaagat attcctactc
    68641 tagctcgtgc cgcaaaaaaa atgtttgaat aaccaagagg ttgtgaaaaa gctgacctgc
    68701 atcaagtaat aagaacaacc aggctaaaac agcttcttat gtttttgatt tggttggact
    68761 tattaccgca gatgcaagct tttgaacacc gtttaccaag aggttgtgaa aaagctgacc
    68821 tgcatcaagt aataagaaca cccaagctaa aacagcttct tatgtttttg acttggttgg
    68881 acttattacc gcagatgcaa gcttttgaac accgtttacc aagaggttat gaaaaagctg
    68941 acctgcatca agtaataaga acacccaagc taaaacagct tcttatgttt ttgacttggt
    69001 tagacttatt accgcagatg caagcttttg aacaccgttt accaagaagt tatgaaaaag
    69061 gcgtttagtt tcgagtatga agaaaaattt tgtgaaaatg gttcctcacg tttttgcata
    69121 aatttgactt aataccgaga aactgatccc ttctgtcata acctcttttt atttatgtct
    69181 gtttcttttt gaaaacaacc attttttcaa cgtttactct cttgccaatc caacatcatc
    69241 taacggatac aactatttca atgattacca ttacactaaa aaatcggaaa cacacctctc
    69301 cgaacacaaa tacataagac tatagaatgt ttcatttaag aaaaaaagcg tttcattatc
    69361 aaaaaggtaa gatctccttt aaaatgaaat tatctgtagt actaataatg cagacaattt
    69421 tatgaatttg aaaggagcat ctccaatgaa agcagtcact tggcaaggaa aacagaaaat
    69481 ggaaatcaga aacgtagatg atccaaaaat attagaacca acagatgcaa tcatccgtat
    69541 cacagcaaca gccatttgcg gctccgatct gcatctttac catcatggtg aagcagtttt
    69601 ggaaaaaggg tatgttgtag gacacgaacc aatgggaatc gttgaagaag tgggatctca
    69661 agttcaaaca ttgaaaaaag ggcaacgtgt ggttatccct tttaatatta gttgtggtca
    69721 ttgtcatttc tgcctgaatc acatggaaag ccaatgcgat gaatcaaatc cagatcagtt
    69781 atatggcggg cttttcggat ttggcaagtt gaacggtaat cattggggtg gtcaagcgga
    69841 atatctaaga gttccttatg cagactctac ttcatttgtt gtaccagata atgttcttcc
    69901 tgatgaaaaa gttttatttc tatcagatat tttgcccaca gcttattgga gcgttgaaaa
    69961 cgcaggagta aaaaaaggcg atacagttgt gattctaggt tctggtccag tcggtctatt
    70021 cgctcaaaaa ttcgctgtta tggctggagc agagcgcgtg atcgcagtag atccagtaca
    70081 gcaccgtcta gataaagcgg ctaattacaa tggggttgaa acttatttct tagaagacac
    70141 ggcacaagct ggaacagaat tgtatgaact ggctaaaggt ggcgcagatg tcattatcga
    70201 ttgtgtagga atggacggat tagagcctgt caaagaaaaa gcgaaaaatt tagtaagctt
    70261 acaaagcggc acaatctctc cattacagat ggcatcgcaa gctgtcaaaa aatttggaac
    70321 agtccaagta actggtgttt atatgactcc agcttcttct tacccgctgc aagaattttt
    70381 tatgcgggat atcgctgtca agcatggaca ggcaccaatt atccatctaa tgccaaaaat
    70441 ttacgaaatg atcgcagaag gactattcga tcccacacaa atcattactc attcgctgcc
    70501 actatcagaa gcagcacaag catatgaaat ctttgacaaa aaagaagata aaaatataaa
    70561 agttgttttg aaaccttgaa ttcagtaaaa aatacaggtg attttcttta tgcccaaaat
    70621 caaacgaaat acccatgaga tcgaaatagg aaaagaagaa gatatcgaaa tacgaggacg
    70681 tcgcttccgc ttctatttcc aaaatcgtta cacattgatc tcactggctg tcgatttatt
    70741 aaccgggatt ttctatattt ttggaagcct tgcttcctta actaaaatcc ccgatcgata
    70801 tggcacctat ttttatttgg ctggtgctat tttcttgaca attcgtccta ttctcagaat
    70861 cgtaaggaac atttttattt acgacgaaaa aaaacaatga agagaacaag caaaaaaaag
    70921 aggcatgaga tttgaaaaaa tctcatgcct cttttttaac cctatcgtat cactttgata
    70981 cagacgagcg gtcgtcccac tttcttcctt gaccattgtc aaaaatgtcc actcgtatcg
    71041 ttcataaaac gtggtatgat ctgtaattaa ataaagtttt tcatacccaa gttgatgcat
    71101 tttttctaat ttccgctaat atccgcttgg ctatcccctg cttacgcttt tccggttcaa
    71161 caaacagggc acaaagattt ggtgtcagat cgggacggtt atggaagtcg ttttcaatga
    71221 cgcccgctcc tccgacgatg gtttcttttt catctaagac cacataccat tgaggaatac
    71281 tttccttatt agcgattcca gtacgaatac tttcctcata cacaacagca ggaactcccc
    71341 attttgatga aaaccacttc gcactttgtt ttactaattc tgggtgttct cttaatttga
    71401 tccagttata tgtaaccaat gtttaaacct ctgactaaaa taaaacgttt acaaaaatta
    71461 tacaagtctt tatgaaaatt agcaatgaca acaatagtga tgtaaaaaaa aacggaaaac
    71521 aatcacttgt tctccgtttt ttgattaatc aagcatctaa agcttctttt cttctttctt
    71581 ggatcgtagc taccttgacg aacggtagat agatcaaaat agaaagaacc aaattgacag
    71641 cggctagtac accacctgca atactttttg ttgccaatac accgccaata attggtggag
    71701 ttacccatgg aggcataacg gtagctgctg gcactaaacc tgtagaagtc gcaaagtaag
    71761 caacactaac taagaccatt ggtgtcaaaa taaatggaat gaacatgatc gggttcaaca
    71821 cgataggtaa cccaaacatc gttggttcat taatattgaa gacacctggc gcaatcgaca
    71881 agtttgttac aaccatatat ggtttgtttt tacgtcctac taaataaata gctaacagta
    71941 aacctaaagt tgcacctgtt cctcctaagt tgacaaagct gtcaaagaaa ggtttgttca
    72001 cgataaacgg tgcgctatgt ccagctgcga ttgcattaac gtttgcttca atggctggcg
    72061 cattgattgt ttgcatcaaa ggatcgacca tattcgctcc gtgtaaacca aagaaccata
    72121 agaatggtgt gataaatgca agcagtaagg cagatggata agagtttgca agtcccataa
    72181 atggttcttg gaccactgta tagaaagaac ctacgatatc atcaacaccg aatgcagcaa
    72241 aaattgcagc aactaaagca aacgcagaga tagtgatcat tgctggtagt aatgaagcaa
    72301 atgcttttgc aactgccggt ggcacaccat caggcatttt gatgatcaat ttttcatttc
    72361 cgcttaaacg tacaaagatt tctgttgaaa taatcccaac aataatagca ataaacaaac
    72421 cgtttgagct catacctaat gctccgccta aagcgaagaa agagcctaaa gaaatcacac
    72481 ctgcggaaac accgtctttt cggtagccgt tagctaaatg ataagcaagc aagaaagcaa
    72541 tcaagacaga aagaatggca aatgtaccgt tccaagcagc agccccgaat cctttccatg
    72601 attcaccacc aaaaattgag ttcatcaatt cttggaaccc gggaatcggt aagttattga
    72661 tcattactgc taatgctcct aagatcatta acggcatact caccataaat gagtcccgaa
    72721 tcgcaactaa atgccgttgt gcaccaattt ttgatgctac aggtataaaa tgtttctcca
    72781 taaatgcaac aaatttatcc ataaatcttc cctcttttct ttactgtaga ttacgcgttc
    72841 aatcgacgat acagatcaat gatctctttt gctaaatctg taaaagctat ggatgtcatc
    72901 agatgatctt gactgtggac tgtcaaaagc gtgacttcaa cgtgatttcc ttgtgcttct
    72961 tgtgtcagca tctctgtttg agagtgatgt gcttggacta aagattcctc tgcatctttg
    73021 attttttcat ctgctaaagt gaaatcacct tttttcgcag cagcaatcgc ttccattgca
    73081 tcgctttttg catttccgcc aaacatgatc agacccatga tggcttctag atttttttga
    73141 tcttccattt attgtatcct ccgataattt ttattatttg tttattagtt tttctgcttg
    73201 gtcgagtact ttttcaccat tcatcatgcc atagtcagcc atattgatta tatctaaagg
    73261 aatgccttta ggtgctagct tttcagcaaa ttgattttgc ataaagcgta cttgcggtcc
    73321 taaaagtaaa acgtctacat ttttagattc tagttgatta tccgcatctg aagcagatac
    73381 agcaaagatg tctgcttcca ttccttttgc ttctgctgcc ttttgcattt ttgtgactag
    73441 gagacttgta ctcattccag ctgaacatac aagcatgatt gttttctttt ccattttaaa
    73501 cacgttcctt tctatactta cacttgtata taagcaagta gcgtgccaac tttaaaagcg
    73561 ctttaaaaag ttgatatcat cacatttaag gtctatacca agttgcatac acactaaaaa
    73621 agaaagtgca cactaaacac acaaattaat ttttattacc ttttttcttt taacgtgcac
    73681 aaaaaaagtt gtgacacaaa acttgtcaca actttttttc gaataaacgg tattcaaaag
    73741 cttgcatctg cggtaataag tccaaccaag ttaaaaacat aagatgttgt ttttactttg
    73801 ctattcttat tacttgatgc aggacagctt tttcacaacc tctcaataaa cggtgttcaa
    73861 tcagctgacc ctggatttta gctcaaaacg ctggattcac aacctctttt atttcaaata
    73921 accagtaaat acaatgaaca tctacaaaaa acttctattt atttatagat ttcgtcaggc
    73981 aaatccaatt ctctcttatc aatcaaagtc tgataccacg aagcagattt tttcaatgtt
    74041 ctgcttcgtt catcgttcaa ttcgattcga actaatccat atcgattctt aaatgcattc
    74101 attggtgaca cacagtcagt gaatgcccat aacatatagc cttcgcaatt tgcgccttct
    74161 tctgttgctt tgatcaaata acgcaaatgc tgtccgataa attcgatacg gtaatcatct
    74221 tgcacgattc ctttctcatc catataccgt tcttcatgtt ctcttcccat accgttttct
    74281 gttatcagcc aagggatatt atgataattt tcttttagat acatggctat atcatacatg
    74341 attttcggat agatttccca tcccctggaa ttattcattt tcttcccagg taaatcaaag
    74401 acttcataat atcgttctgg atggaaaggt gtctcttcat tccattgata acgaggagcg
    74461 cgaacgcgtt taggataata ttgattgatt ccaaggaaat ctactgtatg atgctgaatc
    74521 gtttctaaat cctctgttgc aggatcgaaa taaatcgatt gtttttgaca caactccagg
    74581 aactcttctg tgtagtaccc ttttaccatc ggatcaaaaa atactcgatt ataaaacaaa
    74641 tcatacatat aagcagcttt ttggtcttct ggagaattag atcgagcata gaccatttca
    74701 ggatttaaaa tacagccaat ccttccacca taatttccgc tgcggtaaat ctctactgct
    74761 ttagccgttg ctaaaacttt atgataattc cacatcatcc acttttttgt gttttgttca
    74821 tgcggccagc gaacagcatc caaatagcaa cgcgtttggg gaacgattgg ttcgttgaat
    74881 gtaaaccagt tttttaccct atcaccgtaa cgatcaaagg caatttgagc atacttagca
    74941 taaaaatcaa ctacttttct tgaagaccag ccatcatatt tttccaaaag aaaagctggg
    75001 acttcataat gttccaaaca aatcatcggt tctacgtctg cttcgatcaa agagtcgatc
    75061 atttcactta tatgctgagc atattcttca tctactatac agttctcata atcagtaaaa
    75121 aaacgagccc aattgataga agtccgataa tgtgtcagat ggatggattt catcagatcc
    75181 acatcctgct ggtattgttc cataaaatta gttgctacgg ctgggccata cccattatgc
    75241 caaacaaaat gttcattttt ataccagcta tctataaacg aatcctgtcc ttcttttttt
    75301 cctttccaac cttccgtttg ccaagcagaa gatgcagcac caagaataaa attttttgga
    75361 atagtaattt tcatgatttc ctcctgtttt tagatcccat cattttccac acgatttgct
    75421 acttttacaa atggcagata gatcaaaata gaaacaataa tacaaacaag ttgcgtaata
    75481 actgcaccga tatttcctgc tgttgccaga taagcattga cgatcggcgg cattggccaa
    75541 gggaccatta caactgcttt cgccgcaaac cctaaagcgg ttgcaaaata accaatcgtt
    75601 cctgtgataa gaggcgttaa gataaacgga atagctaaaa ttggattcag catgatcggc
    75661 ataccaaaaa taactggttc attaatgttg aagatcccag gagcaaggga taacttggtg
    75721 atttgtttca tatcctcccg ttttccaact aacaatactg caatcagtaa accgatcgtg
    75781 actccagacc cacccatact catatacata tcccaaaatg gcatagtaat aatgttcggt
    75841 acttcttttc cagcttcaaa agcttccgta tttacagcaa ttgccgctaa aagcaacggt
    75901 tcccgtatcg gcttcaccat ttgatttcca tggataccaa tcacccaaaa gacttgcgca
    75961 ataaacatca atagcaaaat acccggtaac ccttgaacga caccttctaa tgggcgttgt
    76021 acgacattgt aaatgatatc ataaaggtac attcctgtaa gttctttgat gataaatccc
    76081 gcagccgcga tacccgatac agtcagaatc gtcgggaata aagcagaaaa actagcagaa
    76141 acattcgttg gcacggtatc aggcattctc agctgtagtt tcttctgttt cctcaaccag
    76201 caataaagtt caatagaaag gatggagacc aacattccca aaaataaccc tttcgtgtct
    76261 gtatattgtt tagccaatac gttttctact ataacagtct gattttcatt cactaattca
    76321 taaacagttg gattaacaga taaataagac atcactgcga tgataccagg gaatacacca
    76381 tccactttat tcagcttggc taattcgatc cctattagaa aaacagcata gatggtcaag
    76441 aaacttagtg tgacatagct gatggcagat gcgataggct ttaattcttc taaaaaacgc
    76501 aagtaactta tctgtgctaa gccattttcc gagtcaaaga ccattgctga aaacaatgtg
    76561 ccaaaagcac ccgtaataat taccggaatc aatgcagcaa atgcgttttt tatcaccatg
    76621 atatacctta aattattgat tctcgttgcc acgttcccta aaactttggc taatttatcc
    76681 ataaaactca ttccagatcc cctcattcct gcttcttctc cttgacgtca tctctttttg
    76741 tctattagtc agcgcttaca ttttggtgaa cagaaactat ttactcctct tttttaccaa
    76801 atataaaaca aaaaatcatc tgatgtattt atcataaaat acatcagatg aactgtcaat
    76861 tagtttcttt ttctcctatt attttctgta attctctccg atttattgcc atatggacag
    76921 tcattgcgtc aaacgcagca agtacgttcc gcctttcgat ggcctgaacg atctctgtat
    76981 gaatatcgat cgtctcgatt ttcagatgct ggatatcata atttttatta aacaaagcaa
    77041 tcgattcatt aatgattggg ataatatgca gcaaggctat gtttccgcta gcttctgcaa
    77101 tcatcgtatg aaaatcgatg tctaattgaa catgttcttg tgtgccttct cgaaaagaag
    77161 tttcaatctt ttgcgttaat tttttcattt ggacgatttg attcttcgtt gcatattccg
    77221 ctgccaaacc tgccatctta ggctccaata aatatctcag ttcaaataag tctcgtatca
    77281 tccgatcctg atcttcaatc aaagaaaaac cgagtgggtc attggcaatc cctcgcttat
    77341 cactgacaaa tgtccccgct ccttgccgaa cttcaaggat attcctggaa gctagagccc
    77401 gcacagcttc tcgcaccgtg cttcgcccca cctctaaata atcggacaat tcatattcat
    77461 tcggcagttt atctcctact tgcaactgca tttcctttaa atatctaagg atcttgtcag
    77521 tagttacttc tactagggtt ttattttctt ccaaaccaca tacctccttt tcatctgatg
    77581 tattttttcg taattattat aacacgtttg aatcatctga tgattttttg tataaaaaat
    77641 cgattaatcc gttcatagac atacaaacga agaagatatt taccataaaa aagactagga
    77701 taaaattcac tcctagcctt cgatcatttt tattttgatc gacaaaaaat atgatgttca
    77761 ataacagacg atcttcattc gattcttgat gtcatctttt gtttttttta atatatggat
    77821 aaaaagtttg attacatcgt ttctattcta gaatttggtc gattctcaat tcttcttctg
    77881 gtgagaaatc taaattatcc atcattcgaa cactttcttg taactgactt aaccgacttg
    77941 ctccgatcag tacacttgta acagattttt gtcgtaaatt ccatgcaagg gctatttgcg
    78001 ctaatgattg tcctcgtgat acggcgattg tttgcagtgc tttgattttt tccagagttg
    78061 atccaacttg ttcttctgaa aggaatggta tttccttacg atgtgctcta gaatcttcag
    78121 gaataccatg tagataacga tttgtcagca acccttgagc aagcggactg aacgtgattg
    78181 cccctagccc ttcttcttct aatactggag atagatcgtc ttctagtagt gcacgagaaa
    78241 acatattgta gcgcatctga tgaataatga acggtgcttc ctttcttttt aaaatttcag
    78301 tcattttttt gtatcttctc cattatagtt ggatatacca atatataatg cttttccttg
    78361 tcttacaagt tgcatcaacg cttctgctgt ttcttccaaa ggtgtatcag ggtctgggcg
    78421 atgatgatag aagatgtcaa cataatctag ccccattctt tggaaacttt ggtcacaact
    78481 tgatatgatg gatttacgag agccccattc cccatatggc ccttcccaca tatgataacc
    78541 tgctttgcta gaaataatca gttcatcacg gtaggcttga aaatcttctc taaaaatacg
    78601 cccaaagttt ttctctgcag ctccagcagg gcctccataa ttattcgcaa ggtcgaaatg
    78661 ggtaattccc atgtcgaatg cgcctcttaa aatctctctt tgattatgga taggatcata
    78721 atcaccaaaa ttttgccata atccgagagg agagataaaa tcggtaattt caaacctgaa
    78781 tgaccggatt tacgatattc cattttttca taacgtgcac tatctgcaat atacaatttt
    78841 tctcctcctc atcaaatctt tttaaaacgc tttcttgttt tctattttta gtttactaca
    78901 ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat atttttttgc catttgtttt
    78961 ttactcgttg atagatcaga aattgattac gtgcttgctg gattttttta tagcgtttcg
    79021 gttgtttttt gtaaaattgc tgatgatagt tttctgctgg ccaaaacgta ccggctggtt
    79081 cgatttttgt gacgattggt tggtggaaag ttcctgaact gtctagtttc tgttttgatt
    79141 gttccgctaa ttctttttgt cgttcgtctt gataaaaaat cactggacga tattgcttcc
    79201 ctctatcttg aaattgtcca aatgtatctg tgggatcagt aatttgccaa tagattgtca
    79261 gcaactcgct ataacttatt tctcttgtgt cataaatgat ctccacagct tccacatgac
    79321 ctgtgtaacc accacttact tgatcataat tcggtttttc aacatgtccg cccgtgtatc
    79381 ctgacaaaac agataggatc ccttttttcg attcaaacgg ctcgaccata caccagaaac
    79441 atccccctgc aaatactgca cgttcttgat agtcagcatc ttgcatggca tgcttggaaa
    79501 agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa taaataaaag tccattacat
    79561 caggcgtcag attcattcgg atagctaatg gacgtagtgt tacttctagt tttgataact
    79621 gttctttaac ggatatattt tctcttgtgt cattctttgt ctgaatcaat acttttcgtt
    79681 cccaatctcg tgtattcgga ttcaaaatca gattataaag tgtttgaatg aatatttgtt
    79741 cctcatccat agattctcct cctttccgaa gaagacaagc tagtgacttt agctgtaatt
    79801 tatcataaag tgaggaaata atcgattgaa tcgtatttat tcaaagaaga taagcagctt
    79861 cagatgatat aagatgatct ttatcttaaa aagcatactc tatggtttgg tttaactaaa
    79921 aaacgacctc tgaaaaaagg gatgttctcc aattctcaga agtcgagtaa agatgattct
    79981 tttttatgag cgataataaa cttcatccaa aaacagacct tgtgcaggga cggtttctcc
    80041 tgcatgttgc cgcactttgc tttcaaatat ttcatcgatt gtttctatcg gcatggcacc
    80101 tgtgccgatt tctagtaatg tccccatgat aatccgcacc attttatata agaatccgtc
    80161 tccgacaaaa gtaaaatgaa gcatttctcc ctttcgttcg atagataatt catcaatcgt
    80221 acgcactgtg gatttttttg attttttcaa tgcagaaaaa ccaatgaaat catgtgtacc
    80281 cactaatttt tcgcaagctg cattcagctt gtccatatcc aactcttgag ggaaataaaa
    80341 gctgtggttt cgctcaaatg ctgaaggaat cacgttgttc caaacataat agctgtattt
    80401 tttgcccacc gcattatatc ttgaatggaa gcgctcaggc atttcttcga ttcttttgat
    80461 gacaatatcc cgcggaaggt aacgattcat aaagtcgatc atttctgcca gtccaatttc
    80521 tgtgtttgtt ttaaaatttg ctacttgtcc tcgcgcatgg gtgccagcat ctgttcttcc
    80581 tgatccaata atttctatct cttcatttgt catctgatgc aatacttttt cgatttttcc
    80641 ttgaatggtt ttatcgtcat ctccaagtct ttgccatcct gaatatcttt ttccgtcata
    80701 ttcgattgtc aacttgatgt ttctcatatt ttagtggctc ctctgtcttc catttggtct
    80761 tctctctacc tttttaagat tgtagcacaa aatatagctc cttgcttctc taagacagaa
    80821 gctatacaag aaaactgtgg aaatatatat ttcagaaaaa caaaaaacag ccaccggatg
    80881 aagtatccgc tgactgttca atttgacggc tcaagtttta ctgtttgaga tgcaagatac
    80941 tcaaccaggc aatgggcaca taattaaaga aaaaactgat tctttggcaa aaacctattt
    81001 tattcagtaa tggccaagaa tttatagaag gaattcttgc cagtccataa atcgccgcaa
    81061 aaagcaaact gatcacagac agtccaaggt ataagtggct tacgtccatt tgcccatttt
    81121 ttcgatagat caagaaaaga agaactggaa acaataaaaa accactgtaa cctaaacctg
    81181 atccgatatt atggacccat gtcgagacat tccacgaaga ttcatgggta ttgatactga
    81241 acaaacctgt aaagatacaa tcacctatac catagaacgc gatggctgct gtaactaggg
    81301 tagctaacac acgagatgta cttacaaatg cagcatacaa tgcaggcaat gacaggatga
    81361 aaaagatccc tgaaaccaca gaccaaataa gaaacgcttt tcttacagga ctggaaacct
    81421 ctccaaatac actgatcacg ctcttcattt gatctaattc aggataatac aatcctaata
    81481 cgtacggagt caaaaaatca ctcagcacgc ctaataataa aaagaaaaaa ccaaatttac
    81541 gtaaaaaatc catgatacgc ctccaataac aattacctat gcttttaatt gttattataa
    81601 acgactttat cttgatttaa cagaaacctc tatttacacg aacatctata taactcatta
    81661 aaaactcgaa tcattttaaa aatactaaat atctaacgtt caacgttcac ttaatccatc
    81721 taaacaatct ttcaatttga ttttttcccc acttctttta cgcgcgccta gctctcctat
    81781 tgcagtaaac aacagatcac ttgacgaatt taaggcagtt tccattgaat cctggataac
    81841 accgataata aatccaatac caacgatttg catcgcgaca tcattagaga taccaaacaa
    81901 actacatgct aatggaatca ataacagtga accgcccgca acacctgaag ctccacaagc
    81961 agctaaagca gacaaaatac ataaaaggac tttcaaaaca aatggtgctt caatccctaa
    82021 agaatgtaca gcagtcaacg tcatcaagga aatggtaatc gcggcaccgc ccatattgat
    82081 cgtagcacct aacgggatcg atatggaata agactctttt gtcaatttca atctttcagc
    82141 caacatcatg tttatcggga tatttgcggc agaacttcgg gtaaagaaag cgggaagtgc
    82201 gctttctttc aaacaaaaga aaatcaatgg atagggattt tctttaataa acaaaaacgt
    82261 gaggaatggg tacacaacta atgcgacaaa cagcatcgta ccgatcaaaa caagtaaaag
    82321 ctgcagatac tctgccaatc cagcgattcc agtcgtagca attgattgat aaaccaatcc
    82381 taaaatccct atcggagcaa atgcaataat cacttgtacg acttgtgaca acgctataga
    82441 tatctgatcc accaactctt ttgtcgattc tgaacgtgct cgaaaagcta gtcccaacaa
    82501 tacagcccag aataaaacgc caaggtaatt cgcatcaata agagattgaa caggattttg
    82561 tgtgacattc gtcaacatag tagataaaac actttccaaa ctttgcggtg ctttttcaga
    82621 tactgtttct tgtaaagtaa taggaacagt aaaaattgaa cttacaatga ctgctacaag
    82681 tgcagaaagc aatgttccca ctaaatataa gatcaaaata ggcttcacga atgtcttatt
    82741 cccaatttta tgcttggcaa ttgacgccat cgtcaagaaa aaaaccagaa taggtgcgat
    82801 tgccttcaag caactgacaa acaacgtccc caatattgaa ataaatgtcc acgtggggaa
    82861 aaagatacca aacaatattc cgactaaaat accaacaaca atacgttgaa ctaacgacat
    82921 atccaaaaac ttctctaaca aatgaccacc ctttcacaat tttaacaatc attgtatcat
    82981 tttatctaaa gaaactaaag gtgcaatttt ctaaaaattc taataataca attggcaatt
    83041 catgctcttg caacattcaa ttttagtatt ggagggttta ttcaatctct aaagaagtcg
    83101 agagaaaact atttatcgtt attattatag ttatccaagt aaaacgtaac taagatgaag
    83161 gacgaaacta gaaatttcat ccttcatctt aattcttaga catagcaatc aattaatatt
    83221 ttccactgag tctcttcttt ctctcactct cactggttta cttacccata aacgatttga
    83281 ttaaagccat ttttcaattc attattcgct gtctcgttaa agtaaagata attatcggga
    83341 ttcccctgat tttgttgtgt caccaatact tttggctgat tttgctgaaa cccgaataga
    83401 tagacgttct ttttcagata tggttctgtc tctgcatcgg aatagacggc tgtgatttga
    83461 tagtctgctt gacctgtccc actttctgac cattctgcag aaacaggcac tccaccaata
    83521 gccatcttcc actccccgtt caaaataact tgagggactt taagaccata taagtccaca
    83581 ctcatttgat tcgtatagct tttgtattgt tgtcccattg tttggcccca tgtagccata
    83641 aatgcctcta attgtgcgga tttgtctgca ttccacagtg tttgttcgac ttgttcgctg
    83701 ctttctgaag atgaagcctc tacagaagaa cttgaagtag atgtttgagt tgtttcagtc
    83761 gttttggaag actctgacga agtagaggta tctgctgttt ccgtacttgc tgcagttggc
    83821 ttgctctctg tgctagttgt tgtagtagtt gcaattgttg ctttcgctgt ttcttttgtt
    83881 gattcttttt tcgattcatt cccgttggta caaccagcta acaaaaaagc tagaccaatt
    83941 gttactaaaa tccttttttt aaattaaaaa actcctttcg attactccca cttctttatt
    84001 ctatcaaata agcaatgtga ttttattgat tttttgtatt ttctcatcca tcttttaaac
    84061 cttttaaaaa ctaagaagca cccgctgatg ttgtctgtaa tacatcagcg ggtgcttctt
    84121 ttattttaat cttcgtgatt ttccaattta cctcacgctt tatatttgta tttgaagaaa
    84181 catagacttt tagtcatcat cgtcttcctc atccaaatct tgtcggacag tttttccttg
    84241 gagtctttgt acttggtaga tgatttcttt attgaaactt aaaagttcaa caagcaattg
    84301 tttcaagcca tatttttctt ctttttcagc taatttgatt gcccgtgtga taaagacatt
    84361 ttggaaatca aaatcttttg caaagttttc caacattgtt gaggctggta aatatttgtt
    84421 ttgaccatgt tcgtaaattt ttccataacg catgacttct tctaaagtag acggaacgat
    84481 ttcattttca atcactaact catgagagat tttattgaac catctcgctt cttgatcagc
    84541 atattctgaa aaaagggtac ttaattcttg cgaagatgta cccaaaacat acaattgtgc
    84601 ttggatcaat ttcatttctt ctaatttaaa atttgccaat acatgcccca ccatggcgcc
    84661 agcagttggt ttatgatgat cgatatctgc ttgttttgtt tcttgagcaa atttttcttc
    84721 cactatattc actgatttac gcctctttta cttttgttga ttggacttca taacctaatt
    84781 tttccacaac tgcagctaat tgttcagggt ggacttcatc tggatcaaag tttgctttga
    84841 ttttgctcgc attaaacaaa actttcgtat tgcttacccc aggctgttga gaaaccgcat
    84901 gttctatttt ttgcatacag cttggacaag ctagtgtatc taattgcata atcatttttt
    84961 tcatcgtagt tcttccttcc tttttcttga tagtcttatt ctagattaca gcttccataa
    85021 aaaaattgat tcttatcaag ttttacgatt tttcttcttt gctttcgtgt ccaaaataca
    85081 ataaacgcat cgcgttaaag ataacgatca agatactagc ttcgtgaacg aacatcccac
    85141 tagccatgta aataaacccg gcaaataagc cgatgagtaa aaataagacg acaccaatgg
    85201 caatcactac attttcacgg gtattcaaaa cagttttctt agccaaacgg taagcatgaa
    85261 ccaacgatcc aaaactagat tgcatcaaga caacgtcact cgtttcgata gcgacatccg
    85321 ttcctgatcc catagcaatc ccgatatcag ccgcagccaa agagggagaa tcattgattc
    85381 catctccaat gaaagcaacg tgcaatcctt gttcttggta ttttttcaca aattgaacct
    85441 tttcatctgg caataattca gcatggactt catcaatgcc taacatatca gctacataat
    85501 cagcagtcat ctgattatca cctgtcagca tcactaaatg ttttgctcct gcttgtttca
    85561 acatagctaa ttgttcagcg acttctggtc gaatcacatc tgacacacca atgatttgag
    85621 tgattttttt gtcgattgct acaataatga ctgagctgcc tctttgttgt aattgatgta
    85681 aatcttgtac ttgctttggt gtgagagaaa gtttgtgagc ggcaatcaat ttggtgttgc
    85741 ccgcacaaac tgtatgacca tcaatctcag ctgtgattcc ttgtcccttg atcgtctgat
    85801 tatctttgac tgctaattgt tgaaaatcaa tgtctttctc ctgagcataa tggatcacgg
    85861 cgcgccccag aggatgatcg gataacgttt ctaatcgagc cactaaagct aagctgtccc
    85921 cttcatcctc tgtatagttc ttgatagtgg atacagcagt agttcctttg gtcaatgtac
    85981 cggttttatc aaagacaaaa gtatcgattt tggaaaaagt atccattgct tcaccgcctt
    86041 tgactaagat gccccgtttc gctccattac cgattcctgc tacattcgaa acaggcgcac
    86101 caatgactaa agcgccagga caaccaagaa ccaaaacagt gatggctaaa cgaaaatcac
    86161 gactgatcag accaacaagc aacgcaataa gtaaaacggt tggagtataa tactgagaaa
    86221 attgatcaat gaatttttcc gcatgtgatt tggtatcttg tgcttcttcg actaattcta
    86281 taattttggc aaaggtcgta tcatctccga ccttagttgc ttcgatcgtc acgtagccat
    86341 ctgctaaaat cgttccagaa aatacgttat ctccactgat tttgacgact tgtcgtgatt
    86401 cacccgtcac tgcggcttca tccgcatacc ctttcccgtc aatgactcgt ccgtccacag
    86461 gaatagctcc accagttttt actaagacga tatcgccttc ttccacgtca tcgatttcca
    86521 cttcttcggt cgttccatct tcttggacaa ctctggcagt cgatggggcc atctcggtca
    86581 aagccttgat tgattcccgc gttttggtca atgttttttg ttccagataa ctaccaaaca
    86641 aaaacaagaa tgtaacgata gctgactcct tgtattctcc gatcataaag gcaccaagca
    86701 cagcaatagt aacgagtaaa tcaattgaga taactttgac ttttactgcc tgatacgcat
    86761 ggattataat aggtatgacc gcaataatag aagctatggc tagagcaatg ttatagaaca
    86821 agacaaatcc agtaaatttc ccgataaaac caaaaataat caacacagct gataaggcgg
    86881 tgatatgatt tcgatgctct tggatccact tttgtaattt catctttctc cctcttttcc
    86941 ttttcattct tacgagacta agtatagata cttatccatc ataaaaaatt gaccactatc
    87001 aagtttattc aaaaaggcgg ttttcttttg gtatactgaa ttcctacagt cattaaacga
    87061 tcatatagaa aaaggaagcg ccgaaacgct tccttaggta gttctatttt tttattcaaa
    87121 gataagatgc tttgtcatct tacgactgaa gaacaacaat caaaaaaggg cagcagccac
    87181 tcattcacaa gaaatatcgt tttttgatat gggaattgaa gcagtggaca aatatctcta
    87241 cttattagct gggaataaga tccaaaaaag cttaatggat tttatccaag aattggaatg
    87301 tactttccac aaaaaattca ctcattccat tcttttaaaa cttttgattc atacagcgtg
    87361 tctaattgag cgtacattga taaacggtca tgaacttaaa attatatcag aagatgacac
    87421 aagaccttct catgaaacaa tttttcatgt aaaaaaggcc ttcaaaaata ttgaaacaga
    87481 atttgggatc actgtttctt atgatgaatg tttctttatc tatgatatta tcgcatctaa
    87541 ataatctttt tttatgtaaa cgagtatttt tttaacttat tgtttaagag tgatacgcga
    87601 attgtaaaat taagttagaa aaataaaaag gcatttatgg tacactcaaa ttgtatttcc
    87661 gacgaaagaa aacaaaggag tgt
//
LOCUS       DANKEF010000004        86980 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000004 DANKEF010000000
VERSION     DANKEF010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 86980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 86980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..86980
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.004"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(98..1228)
                     /locus_tag="PWZ48_000298"
     CDS             complement(98..1228)
                     /locus_tag="PWZ48_000298"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDL2259980.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="PWZ48_000299"
     CDS             complement(1877..3079)
                     /locus_tag="PWZ48_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2259981.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="PWZ48_000300"
     CDS             complement(3139..3342)
                     /locus_tag="PWZ48_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HDL2259982.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="PWZ48_000301"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="PWZ48_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="PWZ48_000302"
     CDS             4879..5691
                     /locus_tag="PWZ48_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2259983.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="PWZ48_000303"
     CDS             complement(6715..6867)
                     /locus_tag="PWZ48_000303"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2259984.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="PWZ48_000304"
     CDS             complement(7058..7936)
                     /locus_tag="PWZ48_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2259985.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="PWZ48_000305"
     CDS             complement(7975..9093)
                     /locus_tag="PWZ48_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HDL2259986.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="PWZ48_000306"
     CDS             complement(9090..11189)
                     /locus_tag="PWZ48_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HDL2259987.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="PWZ48_000307"
     CDS             complement(11240..12061)
                     /locus_tag="PWZ48_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2259988.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="PWZ48_000308"
     CDS             complement(12058..12867)
                     /locus_tag="PWZ48_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2259989.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="PWZ48_000309"
     CDS             complement(12888..13382)
                     /locus_tag="PWZ48_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2259990.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="PWZ48_000310"
     CDS             complement(13394..13882)
                     /locus_tag="PWZ48_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2259991.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="PWZ48_000311"
     CDS             complement(13959..16655)
                     /locus_tag="PWZ48_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2259992.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="PWZ48_000312"
     CDS             complement(17083..18156)
                     /locus_tag="PWZ48_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2259993.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="PWZ48_000313"
     CDS             complement(18153..18980)
                     /locus_tag="PWZ48_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2259994.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="PWZ48_000314"
     CDS             complement(18981..19787)
                     /locus_tag="PWZ48_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2259995.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="PWZ48_000315"
     CDS             complement(19799..20884)
                     /locus_tag="PWZ48_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2259996.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="PWZ48_000316"
     CDS             complement(20901..21443)
                     /locus_tag="PWZ48_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2259997.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="PWZ48_000317"
     CDS             21701..22690
                     /locus_tag="PWZ48_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDL2259998.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="PWZ48_000318"
     CDS             22687..23424
                     /locus_tag="PWZ48_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HDL2259999.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="PWZ48_000319"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="PWZ48_000319"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HDL2260000.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="PWZ48_000320"
     CDS             23988..24620
                     /locus_tag="PWZ48_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2260001.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="PWZ48_000321"
     CDS             24705..25352
                     /locus_tag="PWZ48_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2260002.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="PWZ48_000322"
     CDS             25357..26340
                     /locus_tag="PWZ48_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HDL2260003.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26743..28257)
                     /locus_tag="PWZ48_000323"
     CDS             complement(26743..28257)
                     /locus_tag="PWZ48_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HDL2260004.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28395..29201
                     /locus_tag="PWZ48_000324"
     CDS             28395..29201
                     /locus_tag="PWZ48_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260005.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29376..29945)
                     /locus_tag="PWZ48_000325"
     CDS             complement(29376..29945)
                     /locus_tag="PWZ48_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260006.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30028..32088)
                     /locus_tag="PWZ48_000326"
     CDS             complement(30028..32088)
                     /locus_tag="PWZ48_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HDL2260007.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32365..33843)
                     /locus_tag="PWZ48_000327"
     CDS             complement(32365..33843)
                     /locus_tag="PWZ48_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260008.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34141..35076)
                     /gene="murB"
                     /locus_tag="PWZ48_000328"
     CDS             complement(34141..35076)
                     /gene="murB"
                     /locus_tag="PWZ48_000328"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDL2260009.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35149..36069)
                     /locus_tag="PWZ48_000329"
     CDS             complement(35149..36069)
                     /locus_tag="PWZ48_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2260010.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36182..36934
                     /gene="xth"
                     /locus_tag="PWZ48_000330"
     CDS             36182..36934
                     /gene="xth"
                     /locus_tag="PWZ48_000330"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDL2260011.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            37798..38331
                     /locus_tag="PWZ48_000331"
     CDS             37798..38331
                     /locus_tag="PWZ48_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HDL2260012.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
     gene            complement(38821..39345)
                     /locus_tag="PWZ48_000332"
     CDS             complement(38821..39345)
                     /locus_tag="PWZ48_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260013.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(39338..39811)
                     /gene="tsaE"
                     /locus_tag="PWZ48_000333"
     CDS             complement(39338..39811)
                     /gene="tsaE"
                     /locus_tag="PWZ48_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDL2260014.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(39934..40914)
                     /gene="pta"
                     /locus_tag="PWZ48_000334"
     CDS             complement(39934..40914)
                     /gene="pta"
                     /locus_tag="PWZ48_000334"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDL2260015.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(41067..41747)
                     /locus_tag="PWZ48_000335"
     CDS             complement(41067..41747)
                     /locus_tag="PWZ48_000335"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDL2260016.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            41873..42742
                     /locus_tag="PWZ48_000336"
     CDS             41873..42742
                     /locus_tag="PWZ48_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2260017.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            42768..43283
                     /locus_tag="PWZ48_000337"
     CDS             42768..43283
                     /locus_tag="PWZ48_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260018.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(43330..43923)
                     /locus_tag="PWZ48_000338"
     CDS             complement(43330..43923)
                     /locus_tag="PWZ48_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260019.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(43948..44127)
                     /locus_tag="PWZ48_000339"
     CDS             complement(43948..44127)
                     /locus_tag="PWZ48_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260020.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(44204..44713)
                     /locus_tag="PWZ48_000340"
     CDS             complement(44204..44713)
                     /locus_tag="PWZ48_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260021.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(44809..45189)
                     /locus_tag="PWZ48_000341"
     CDS             complement(44809..45189)
                     /locus_tag="PWZ48_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2260022.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            45519..46268
                     /locus_tag="PWZ48_000342"
     CDS             45519..46268
                     /locus_tag="PWZ48_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HDL2260023.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(46452..47729)
                     /locus_tag="PWZ48_000343"
     CDS             complement(46452..47729)
                     /locus_tag="PWZ48_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260024.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(47808..48452)
                     /gene="pcp"
                     /locus_tag="PWZ48_000344"
     CDS             complement(47808..48452)
                     /gene="pcp"
                     /locus_tag="PWZ48_000344"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HDL2260025.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(48651..49595)
                     /locus_tag="PWZ48_000345"
     CDS             complement(48651..49595)
                     /locus_tag="PWZ48_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HDL2260026.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(49597..50283)
                     /locus_tag="PWZ48_000346"
     CDS             complement(49597..50283)
                     /locus_tag="PWZ48_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HDL2260027.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            50757..51077
                     /locus_tag="PWZ48_000347"
     CDS             50757..51077
                     /locus_tag="PWZ48_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HDL2260028.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            51231..52136
                     /locus_tag="PWZ48_000348"
     CDS             51231..52136
                     /locus_tag="PWZ48_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260029.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            52133..52906
                     /locus_tag="PWZ48_000349"
     CDS             52133..52906
                     /locus_tag="PWZ48_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2260030.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(53087..54511)
                     /locus_tag="PWZ48_000350"
     CDS             complement(53087..54511)
                     /locus_tag="PWZ48_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2260031.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            54722..55168
                     /gene="nrdI"
                     /locus_tag="PWZ48_000351"
     CDS             54722..55168
                     /gene="nrdI"
                     /locus_tag="PWZ48_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDL2260032.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(55233..57215)
                     /locus_tag="PWZ48_000352"
     CDS             complement(55233..57215)
                     /locus_tag="PWZ48_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2260033.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(57229..58125)
                     /gene="murQ"
                     /locus_tag="PWZ48_000353"
     CDS             complement(57229..58125)
                     /gene="murQ"
                     /locus_tag="PWZ48_000353"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDL2260034.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(58128..59189)
                     /locus_tag="PWZ48_000354"
     CDS             complement(58128..59189)
                     /locus_tag="PWZ48_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2260035.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(59400..59966)
                     /locus_tag="PWZ48_000355"
     CDS             complement(59400..59966)
                     /locus_tag="PWZ48_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2260036.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(59994..61496)
                     /gene="eat(A)"
                     /locus_tag="PWZ48_000356"
     CDS             complement(59994..61496)
                     /gene="eat(A)"
                     /locus_tag="PWZ48_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2260037.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(61754..62875)
                     /locus_tag="PWZ48_000357"
     CDS             complement(61754..62875)
                     /locus_tag="PWZ48_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2260038.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            63001..63942
                     /locus_tag="PWZ48_000358"
     CDS             63001..63942
                     /locus_tag="PWZ48_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2260039.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(64092..64988)
                     /locus_tag="PWZ48_000359"
     CDS             complement(64092..64988)
                     /locus_tag="PWZ48_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2260040.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            65257..66597
                     /locus_tag="PWZ48_000360"
     CDS             65257..66597
                     /locus_tag="PWZ48_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2260041.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            66815..67258
                     /locus_tag="PWZ48_000361"
     CDS             66815..67258
                     /locus_tag="PWZ48_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2260042.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(67762..69180)
                     /gene="nhaC"
                     /locus_tag="PWZ48_000362"
     CDS             complement(67762..69180)
                     /gene="nhaC"
                     /locus_tag="PWZ48_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2260043.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(69583..70806)
                     /locus_tag="PWZ48_000363"
     CDS             complement(69583..70806)
                     /locus_tag="PWZ48_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HDL2260044.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFLYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(70837..71676)
                     /locus_tag="PWZ48_000364"
     CDS             complement(70837..71676)
                     /locus_tag="PWZ48_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2260045.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(71693..72373)
                     /locus_tag="PWZ48_000365"
     CDS             complement(71693..72373)
                     /locus_tag="PWZ48_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260046.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(72375..73448)
                     /locus_tag="PWZ48_000366"
     CDS             complement(72375..73448)
                     /locus_tag="PWZ48_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260047.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(73504..73742)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(73785..74018)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(74095..74766)
                     /locus_tag="PWZ48_000367"
     CDS             complement(74095..74766)
                     /locus_tag="PWZ48_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2260048.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(74853..77531)
                     /gene="mgtA"
                     /locus_tag="PWZ48_000368"
     CDS             complement(74853..77531)
                     /gene="mgtA"
                     /locus_tag="PWZ48_000368"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDL2260049.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            78190..78627
                     /gene="ndk"
                     /locus_tag="PWZ48_000369"
     CDS             78190..78627
                     /gene="ndk"
                     /locus_tag="PWZ48_000369"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDL2260050.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(78649..79515)
                     /locus_tag="PWZ48_000370"
     CDS             complement(78649..79515)
                     /locus_tag="PWZ48_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2260051.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(79675..81768)
                     /locus_tag="PWZ48_000371"
     CDS             complement(79675..81768)
                     /locus_tag="PWZ48_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2260052.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(82517..83140)
                     /locus_tag="PWZ48_000372"
     CDS             complement(82517..83140)
                     /locus_tag="PWZ48_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260053.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(83299..84696)
                     /locus_tag="PWZ48_000373"
     CDS             complement(83299..84696)
                     /locus_tag="PWZ48_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2260054.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(84728..85699)
                     /locus_tag="PWZ48_000374"
     CDS             complement(84728..85699)
                     /locus_tag="PWZ48_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260055.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            86073..86498
                     /locus_tag="PWZ48_000375"
     CDS             86073..86498
                     /locus_tag="PWZ48_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260056.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
BASE COUNT        25527 a        18067 c        15024 g        28362 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctatcga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccaagacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
    26521 cccacgctct ttttccgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca
    26581 accaagttaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
    26641 cagctttttc acaacctctt cactaaacag tgttcaatca gctgaccctc ggtattagct
    26701 caaaacgctg atttcacaac ctctttttat ttacgttcta tttcattcat tttccgaagc
    26761 aaatcgagct gcttgtatgc ccgcttgtcg cccaaaaata atgatctccg caactgaatt
    26821 tccaccgatc cggttgtttc catgtaagcc acctgtgact tcaccagcag catataaccc
    26881 cctaattggc tggttttctt tatctaatac ttcagtgttc gtattgattt tcacaccgcc
    26941 catcgtgtaa tggattcctg gtgcgatttt gatcgcataa taattcggag acgataagtc
    27001 atgttccatg gctgtggcac ggttgaattg agcatctttt ttctccgcaa catccgtatt
    27061 ccatccatca actgtttttg ctagtgtttc ttttggtaaa tcgatttttt ctgctaattc
    27121 ttccattgtc tttccagttg tgacatatcc tttttggtca taaaattcaa tagctgtggc
    27181 acgatcacgc acgccttggt caaaaatcaa ataagctgat ttttcaggca atgctgtaat
    27241 tgctgcagat actttatccc ttgtatccat ttcattgaca aatcttttcc cctcttgatc
    27301 gaccaagatt gctccttctc cgcgaacaac ctcaccaatc aagaccccct catcctgctg
    27361 cacagttgga tgaatttgta ttttatccat atcaacggtt tgtccaccta atttttcaat
    27421 catggtgata ccatctccag ttgatccttc ttgatttgtc gtaatataat cagctaaatc
    27481 gggacgatat ttttcaataa attctttgct ggcaccaaaa ccgcctgtgg taactacgat
    27541 tgcttttccg gtgacctctt ttggttcttc tccttgaaca tgtacagtca cttcattgac
    27601 cgtttcccca tttttcttaa tgtctgtcac atctgcgttg acaaaaatag ggatttcccg
    27661 ctcatggacg ttattcaata acccctccac taagtatcca ccaatagccg agccatcaga
    27721 cggacgatgt gtcctctttt cactcatccc tccagtgatc gttaagttgt ccagtttgat
    27781 accgttttga tctagccaat cgaccgcatc agcagagtga tcgacaaagt atcgaagcat
    27841 ttctttatca ttcgttcctc caccaccttt tagcgtttct tcatagaagg catcattcga
    27901 gtctttgatt ccttcttctt tttgaaattt tgtttctgaa gcattcatac cgcttgatga
    27961 tttagatgtg tttcctccgg caacaggcat cttttccaaa attactggat tcaacccagc
    28021 atcttttgct tcgatagctg ctgccatccc tgcaccacca gctccaataa tcacgacatc
    28081 ataagattct ttcatttctt ttggatctgt atatacttgt tcagaagcgc ctgatgatac
    28141 ttcggcagat tccttcgtac tagaacttgt ctgcgctgta tttttctgat ctgcgccgca
    28201 tgcgctcata gcaagaagtg caacggctgt taataaacca gcaactaatt ttttcatttg
    28261 tatccccctt ttctccttat actatattaa gtgatttttt tcacaatatc aactctgttt
    28321 ttctttaagt catatagctt tggtaaacac acgcttctta gtataattaa ggatatacat
    28381 gaaggagtta ttttatgaat catttgaaaa ccttttttac gaacagtaca aaaactgcag
    28441 gtagaatcac aaacggaatc tatattttat tacgtatcat cgtcgttatt atgctagtgg
    28501 atcaggtctg gaaccaacac tggggaaata caatgctttt attactgaca ttaattttat
    28561 ttgaaatccc ttattttctg gaaaagatat taaagataca gattccaaat atgctagaaa
    28621 tcattttgtt atcttttatt tttagttcaa cagttttagg agagttaagt gatttttatg
    28681 gctatttttc aatgtgggat acttttctac atgcattcag cggattttta gctgggggtg
    28741 tcggcttttc ccttgtttat ttgctcaata aaaatacaaa aaccatgaac ttgactcctt
    28801 tattactagc agttgtttcc ttctgttttt cgatgacagt tggtgttatg tgggagtttg
    28861 tggaattttc agcagataaa ttactgcaat tcgatatgca aaaagatacg atcatccagc
    28921 aaatcaatag tgtggagtta agtaaagacg gaaacactgt ctatcatgtt ccagatatca
    28981 ctcggacaat cattgaaagt caaaatgcag atggtaaaac agataaaaca atcatccaaa
    29041 atggttactt agacgtggga atcatggata ctatgaagga tttatttgtt aattttattg
    29101 gagcagttgt tttcagtgta tttggttacc tctatagtcg aaacgattac caaaaattta
    29161 cgttcatcaa gaattttatt cctaaaaaaa tcaaacgata aaacatgcta aaaaacaaga
    29221 gaaggggcag tgacaatact tttgtcactg ccccttcttt tttgaatgta tggcatgcca
    29281 ttagttatcc gatagtatta actcaaaaaa gtaagatatg aggaatgact atttcgtttt
    29341 ttatcccaat actgaggact tatttcacca tttcgttatt tatctaattt acttaaaacc
    29401 tgcataactt tttcataatc tggattaggt gtgccgtcta gattttcagc aataaaagta
    29461 aaacccattt ttttataaag agcaatcgcc tgatggctcc atgtctgtgt gtgaagatag
    29521 attggtccgt ccgtatggag ggtaggaaat tcttgcaaga ctctggcagt caatgctttt
    29581 gcgagccctt ttccttggta atctggtttc actgctaacc aatgaaaaac cggatacact
    29641 tgctctcttg atttttttcg ccaagcggta caggttgcta ctttttctcc tttttttgtc
    29701 tctacaaaaa acatccttct ttcaagttct tcaggataag gagcaaagtt ttgttgaaaa
    29761 tactcaagtg cttcatctgt cgtttgaaat tccccaacgg aagtctcaat ctccgcccaa
    29821 gcagattcat cttctttttg ataaaatcga aattcaaaac cttccgctaa aggcgtgatt
    29881 ggaacatttt gttgaagtgg tcttgtcatc cagatttctg cgtaaggtaa cgttttatct
    29941 aacatttttc tatccctcct cgtctccatt atgccaaata acatatgact cagctactta
    30001 tttgactata acagatagag atataaatca gaaccagtca tctgcgtcaa attcatttcg
    30061 attagcgtat acccatgttc ctgccatacc tcgcatgaaa tctcctaaag cagaagaagg
    30121 aatacctcca ccaggtgcgg gtgttcccgg actaggaagt ggtacttgcg gctgcagatg
    30181 ctcatcggtg gaatcaatag ttgacaagcg ttttttcttc gtttccttct ggtcatctga
    30241 ttccttctga tcttccgact gtttagcaac aaattctttt tctgattgtt gttttatttt
    30301 aggctctaaa atttcatttt ccaatccaga aacttgtggg gccaaatgag cttcttgcct
    30361 tatcgtttta ttagattctg agatttttct agatattctt gctaactcta ggcttcttgt
    30421 ttgttttact ttccctgttg gagaaactgc atgagccatt tgttgttttt tttctgacaa
    30481 gctttcttct tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg
    30541 taatagtgac tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat
    30601 cgtaaaatca cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt
    30661 aaccgataca tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa
    30721 ctcttctttg tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg
    30781 attgtctggt atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt
    30841 catcagttct gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc
    30901 gtttgcttgc atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa
    30961 atctatattt ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt
    31021 tacactactc atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg
    31081 agtgatgaca gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc
    31141 cacatttgat gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt
    31201 agtatctact gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt
    31261 tgttacatat tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc
    31321 ttttcctttg atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt
    31381 ttttaaagca attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt
    31441 atccacgata ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc
    31501 gttcttcagc ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat
    31561 tttctttctt tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc
    31621 accgtccttt atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc
    31681 accttttgta ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc
    31741 cgatgtccat aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact
    31801 gactttatta taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt
    31861 atgagtaata gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat
    31921 ggtttgtgtc gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag
    31981 catcgtgcgc atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa
    32041 acgacaagcg tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc
    32101 ctccctactc ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa
    32161 taaaatcaaa agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa
    32221 gaaaatcgga ataataactt atgtactttt taattttgtt aagtaatata tctagttata
    32281 tctagttata tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg
    32341 agacaaagct gccatccata aatactagct gattttttca gttaaatgtc tatttttttc
    32401 tctgatgatg gtatttccaa aatattccca gtcatctgga gtcggtggat ttttccaaat
    32461 tatctgttct ttattaaatt tttcaagtaa acaatcagat aaataaatat tcgtacgaga
    32521 agtgattcga ctctcaatca caatattgta atttttaaat cctttcactt gagaaataat
    32581 gtaatcggta taagcagatc cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa
    32641 atatgatacc ggacaaatac cgaccaataa aaagagataa tcataaaata agcgaatcat
    32701 gaaacgttca tcttttccca agaaatagcg tttattctct tgtatgacct tttggattgc
    32761 tgtactgaat attggatacg tctcgaagaa aaactgtagc tgctttttag acgtaaagct
    32821 agtcatctca aagtcgaata tccaattttt tcgattgaca tgcatcatac ccatacgaag
    32881 ttcttttcgt atatatagtc tttcttcttc agagatttca atcgaaaatg gtaattgatc
    32941 aatcaattcc actgacattt tttgactaat catgtggata tcatgaaaat gagcttccgc
    33001 aagtccaaat gcgccatcta attcaaattc ttcttccgtg atcaaaaaca acatgatata
    33061 agaaatctca tcaatgatct gctctttagg gatatgaaga aaagaaatat gtgataaatc
    33121 cttgatgatc ttcattcctt ctgagctctt aaagtattca tctgaaaaga aacgttttcc
    33181 gattggttga ttcccatagg ccctcaacaa actaatagtg gttagcaaat tgaatttgat
    33241 tcgttttgtt gcagacagtt ttaaattgaa cagatattca aaatattgca cgatactctt
    33301 tgcttcattc atgacgcttt ctctaaatgg catagaaaaa ccattaaaga ccccgaaata
    33361 taccccaaat aatacgttcc gtatgtttat ttcttcccca actaatatgt ttttctttaa
    33421 ctttatttga tgctgttcaa gaaaagagac taattctctt ctttttacat aggctcttga
    33481 tatgcttaaa aaatgattct ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc
    33541 cgttaacagt cgagcaattt ttgatcgttg aaagtagatt cttcttaaat ttttgatatc
    33601 cgtcaaattc agacgttcga catggatctc tgcttcttct gaaatttgga tctctggctt
    33661 tgttttgaat tcagtaaatt ctttatttaa ttctatgata taatttcttg ttttaaattt
    33721 agacaaacct aatgtttcat ataacttagg cagcactaca taattctttt tctcaaattc
    33781 taaatatcta gctagcagca gtttttctct atcttcctct tctataaggt aatccaactt
    33841 catatcctac acctcaaaaa atgcctcata ccaattactt gatatgcaga tgaaagttca
    33901 tcttttcacc tatactgatt ttatgatgga ttatgcttgt tttttcatca gttattctca
    33961 ttttttatct tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct
    34021 atattttttg ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca
    34081 caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta
    34141 ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac
    34201 ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa
    34261 ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc
    34321 agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc
    34381 tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg
    34441 ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc
    34501 tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg
    34561 acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa
    34621 aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata
    34681 aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc
    34741 tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt
    34801 tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc
    34861 atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg
    34921 gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc
    34981 tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat
    35041 cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca
    35101 aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg
    35161 acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg
    35221 tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat
    35281 tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt
    35341 tccatcagca tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt
    35401 acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc
    35461 cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct
    35521 tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag
    35581 atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt
    35641 ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa
    35701 gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc
    35761 acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc
    35821 tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc
    35881 gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt
    35941 ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg
    36001 ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc
    36061 aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac cattttttac
    36121 taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt
    36181 attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt
    36241 agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc
    36301 aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa
    36361 aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat
    36421 gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt
    36481 tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt
    36541 agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat
    36601 cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa
    36661 tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa
    36721 cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg
    36781 gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac
    36841 gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag
    36901 tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt
    36961 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata
    37021 ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc
    37081 atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct
    37141 tattaccgca gatacaagct tttgaacacc gtttattaag aggttgtgaa aaagctgcac
    37201 tgcatcaagt aataagaaca gccaaacaaa aatagttcct catatttttc gtttggttgg
    37261 gcttattacc gcagatgcaa gcttttgaac accgtttatt aagaggttgt gaaaaagctg
    37321 cactgcatca agtaataaga acagccaaac aaaaatagtt cctcatattt ttcgtttggt
    37381 tggacttatt accgcagatg caagcttttg aacaccgttt attgagaagt tgtgaaatca
    37441 gcgtcctgac ctgagtatta gagaaacaaa tggcaaaata gttcctcata ttttgctatt
    37501 ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagaa gttgtgaaaa
    37561 agtcgcctac tttcgagtcg taagaaattt ttttgaaaat ggttcctcac aactttgcat
    37621 aaatttgact taataccgag aagtcatgat ccccatctaa ctccaaatca caaggaatga
    37681 gttcaaaaac tagcttttct caacaaatat tacataaaaa attctatgtt ttttgttaaa
    37741 tttatttgct tttttcaaaa gtatgttaca gtaatttccc aaggaggttt tacagaaatg
    37801 aattttgttg ccatggattt tgaaactgcc aatcatcagc cttatagtgc ttgttctctt
    37861 gcgctagtca tggtaaaaaa tagccagatc gttgatgaat tctatacatt gatacagcct
    37921 gagactcctt ttttctggcg aaatgtccaa attcacggaa tccatcaaga agacgtacgc
    37981 aatgcgccga aattcccaga agtctggcaa actatccaac catattttca aaaaaatcgt
    38041 ttagtcgttg cacataatgc tgcttttgat acaaaggtct tagcgggatg cttagattat
    38101 tatcatcttg aacagcctaa ttatctttct ttatgtacag tgaaaagtag ccgtcgcctg
    38161 tttcctgaat ttccaaatca tcgattgaac actgtgtgcg aaaatttgaa catccaatta
    38221 gatcatcacc atgatgcatt ggaagatagt cgtgcatgtg cggaaatcct gctttatcaa
    38281 gaaaaaaaat tcgggacaga tccattgaaa aaacttgtga ctataaagta aaagaatact
    38341 tatgaaacac acaaagaggt cgtgagcctg ccgtttcact ccgagtcaca aggagcaatt
    38401 tcaaaaaata gcttctcatg tttattaaat attgtgactg gtgaccgagg agctggcagg
    38461 agaacaccgt ttattggaga ggttgtgaaa aagctgttct gcatcaagta ataagaacag
    38521 ccaaacaaaa atagcttctc atatttttca tttggttgga cttattaccg cagatgcaag
    38581 cttttggaca ccgtttacca agaggttgtg aaatcagcgt tttgacctga gtattagaga
    38641 aacaaacggc aaaatagttc ctcatatttt gccgtttttt gagctaatac cgaaggtcag
    38701 ctgattgaac accgtttatt caaaaaaaat ggactgcgac acgacttttg tcacagtccc
    38761 ttcgtgtgtt tttctgcctt ctcaaaagga tgacagtttc tcactctttt cctatttctt
    38821 ctattcaatc aaataactca ttttatatac atctaaaaat tcacctagat ctgttcttgc
    38881 ccctctaggc atgaccgctt cgatttggaa gcccattttt cggtaaaggt gtacggctct
    38941 ttcattacga acttgaacgt caagctccaa tcgaaaaagt acacccattt ccttcgccca
    39001 actgatgatt tcttctagca taagagtgcc tagtcccatt ccccaatatt cctgcagaat
    39061 actgataccg acttctccca cgtgagacaa acgaaattga ctgtctgcct tgacagatgc
    39121 tgttccaata atcgtccctt catagattgc aagcagcaat aaattattat tgctttcgta
    39181 tatataatcc aactcttctt ctaaggtttc tggtggtaac aggagttctg cttcatccaa
    39241 aactaaaaat tctgtctcct tattaacttg tcccatcatc aaaagaatgt cttttgcatc
    39301 tcctggcact gcttcccgca agatgatccc aacttcttca ctcatgattt tcagcccatt
    39361 tttctagaat tcgctgtttg aaggtttcac tttgctcccc gtatgagatt agtttcacat
    39421 accgcttttc taaatctgta tcactttttt ctaaaatcag ttccaaataa tcctccggca
    39481 gtgcttcttc taatagattt ccccattcaa caacggaaag cccatcacct tcaaaatatt
    39541 catctaaccc taaatctgct ccgctagctg ctacccgata aatatccata tgatacaacg
    39601 gaatccgacc ttgactatat tctctaataa tcgtgtatgt cggacttttg atcatttgcg
    39661 ttatccctaa tccttgggca attcctttcg tcaacgttgt tttacccgca cctagatcac
    39721 cagtcaatat cagattgtca cctggcattg cggaatgacc aattgcctct gcgaattgtt
    39781 ctgtcattgt taatcctgat agttcgatca tttttcatct tctcccgctc ttctttcttt
    39841 ttgccagtat atcgcgtaat gaagtttttt taaagaaaaa atgaactagt caagaacagc
    39901 agctgctgtt tttggctagt tcgcgtgtat ttgttaatca atcaatgttt gtgctgctgt
    39961 gataatcgat aatttatata catcttcttc atttgctcca cgagacaaat cagaaaccgg
    40021 tttgttcaag ccttgtagaa ttggtccgat tgcttcaaag ttaccgaaac gctgagcaat
    40081 tttatagccg atatttcctg actgcaattc tggaaataca aagactgttg cttgaccagc
    40141 tacttctgaa tctggcgctt tcagttgtgc aactgaagga acataagaag catcaaattg
    40201 caattcgccg tcgatcatgt attgtggcgc tagtttttta gcgattttag ttgcttcaac
    40261 tactttgtct acttctggcg atttcgctga gccttttgtc gagaagctca tcattgctac
    40321 ttttggttcg atatcgaaca attctgctgt ttttgcagaa tctacggcga tttcagccaa
    40381 ttcttgcgca gttggattca cgttgatcgc acaatccgaa aagacatatt tttcttgatc
    40441 tcttccacgt accatgatca tcgctccgct tgtccgactg atgccaggct ttgttttgat
    40501 gatctgaagt gctgggcgaa cagtgtcgcc agtcgaatgg atcgctccag agaccattcc
    40561 gtctgcaatc cccatatacg taagcatcgt accaaagtaa ttcacatctt ttaaaatctt
    40621 ttctgcttgt tctttggatg cctttccatt tctacgttca acaaaagtat ccaccatttc
    40681 atcccactta tcgtaattat cagggctgat gatcgtaaag ctagatgttt tgattcctct
    40741 ggcatgcgct gcatttctta tttcttcttc gttccctact aaaatgggtt ccacaagttc
    40801 ttctgctttc aaccgggcag ctgcgcctaa aatacgagca tcagtagctt ccgggaaaac
    40861 gattttgatc ccacggcgaa caattttaaa tcttaaacta tcaaacaatt ccacaagatg
    40921 acctccagac gtttttatat gtattatcat acactattcc agaaaaaaag aacagtttat
    40981 tttatctgta tcagtcaaaa atgctggaaa aattaagttt ttcacataca aagcgaactg
    41041 cactataaca ggaaagcgct gttttattac actgtttcag gcagttgcca atcaatcggt
    41101 tcttctccta acgcaaggag cgcatcattc gttttcgaga agggtttgga accaaaaaag
    41161 ccacgacttg cagataacgg actgggatgt ggagatgtta tgatgatatg gcgactctta
    41221 tcgatcattt tgattttttc ttgtgccggt tttccccaca agataaacac gattggtttt
    41281 tcccgttcat ttaatttttc aatgatcgtg tctgttaatc tttcccaacc tttcccgcga
    41341 tgagaatacg cttgcccttc ccgaacggta agaacggtgt tgagtaaaag gactccctgt
    41401 ttagcccatg acactagatt tccatgttga acaggtgcga ttccaagatc atcataaagc
    41461 tctttataga tatttcttaa cgatggcggg atctttacgc caggttgcac agaaaaagat
    41521 aagccatgag cttgattttc tccatgataa ggatcttgtc ctaaaatcac cactttaact
    41581 tcttcataag gagtcaattc caatgcttca tagatatgat acatatctgg atggatcttt
    41641 tgggacgcat attctttttt taaaaattct cttagatgca gataataatc ttttgaaaat
    41701 tctgaggaca aaacttcttg ccaactgtta tgaatgattt ctttcaaaaa ctccacctcc
    41761 taataaaaag aataactcac attttcttca atcacaaggg acaatcttca ccctcagcac
    41821 ttgattaata tggtaaacta ggaacaagaa gcagaaagga acgataatca ttatgatcaa
    41881 attaattgca tcagatatgg acggcacatt attagatgcc catatgagtg tttctcctga
    41941 aaatgccgag gcgatacgtt ttgcaaatga tgccggtgtt gagttcatgg tcgctactgg
    42001 tcgaaactat caagaagctc gcgcagcttt agatgaggta ggaatcgatt gtgcgatgat
    42061 cactttgaac ggggcacagg tatttgataa agaaggaaat tcattgttta ccgtgtcgat
    42121 tccaaataat caagcaatct ctgttttaga tattctagat gctaacggta tttactatga
    42181 agtagcaacc aacgatggtc tttattctga aaatcaagcg aaacgaattg aaagttttgc
    42241 ttccatggtg gctacccatt tacctcacct tacgtataaa atggcgattg ccatggcctc
    42301 agctaagctg gagttattgc acatcacata cgtggacagt attcgtgcta ttttggaaga
    42361 aaagaatctg gaagttttaa aaattatttg ttttcataca gaagggccag ctgtattagg
    42421 tccagttgga aagcagattt ctcttttagg cgatctagcc gttacttcct ctggtcaaaa
    42481 caatatcgaa gtgaaccata aaaacgctca aaaaggaatt gctgttgccc atgtcgctca
    42541 cgagcgtggg atttctttag aagaagtcat gacgattggc gataacttca atgatgtcag
    42601 catgctgcaa gtagcaggag tcagtttcgc aatgggaaat gcagaaatcg aagtaaaaga
    42661 ttatgccaaa tatacaacgg acacaaacct tgaatctggc gtaggaaaag ccatattgcg
    42721 cgcgattgca gaagacttgt agttttatct agaaaagagg gatatagatg tcagaatttt
    42781 tcattcagga aaagcagctt ggacgtgcaa ctcggacaat tgtcaaaaac gaaaaaggac
    42841 attccctgtt tttgatggtt ggtcgctggg gaacaaaggg cgatgcattg tctctttatg
    42901 ccatgaatgg cgatctcgtt gctcacatca agcagataag cctgacattc ggtacgcgtt
    42961 ttgagctgta taaagagttc gaaaaggtgg gaaccatgca gaaaatattc aattggcccg
    43021 gtgactttta ttatattcgt agacttcact ggacagtcca aggtgatatc tataatcatc
    43081 aatatcaaat ccatcatttc aatcagcaaa tcatgaaaat ggataaagca acactttttt
    43141 caggagatta ctatgtattg gatgtctcaa atgatgaaga tgctccagtt tgtatttgta
    43201 ttgctgcggt tttagattac tggctctacc gtaaagacaa aagtcgcgat aagcctgttt
    43261 cttaccgact taacttaagt taaaaagatg tacctgaaat gcggaaatac gcatttcagg
    43321 tacatctttt tagatattct tttgcatatg atcataaaga tgaccactga tcatccgctg
    43381 accgacaact ttgaagtcat ggcgttcgta taatttttta gcctttggat ttttttcatc
    43441 cacacttaga ccgatgacgg agcgattagc tttcttcgcc aatttaggta atgcttctaa
    43501 taatcgcgaa ccgatgcctt gtcctctaaa atcttctgat acactgatag agtccaaata
    43561 ccattcgttg ggaaacgttt ctttatcaat gaacaaacgt gtagacgcat ctagctgata
    43621 tttctctaag ataggtacca gtggctgatc gatgattgat tcttcttcat ctgtataacc
    43681 gtaagcaact cctgcaactt taccgtctat ttcttctacg atacctcgtg aataactgta
    43741 acgataattt ggatcagtga ttgcttcttc taaaatctct aaagtctttt gttcccctgt
    43801 ctgcttcaaa aatggcagtt ccatatcttt tagaatcact aagatcaatg gggcgatttc
    43861 tggagcatct tcttttctcg caaaacgaat catagtttcc tctccttttc tccattctaa
    43921 catgcagaaa aagaaatcgc aaaatcgtta ttttaaaaaa tcttcaatct tttcagtaat
    43981 gaaataacca tgagtaacga ttcttgccgc ttcagttcgg caataagggc agacgactgg
    44041 catttcttta tcattatgtt cgactacatt attgacttct tcgtatgcaa atttttttcc
    44101 gcaattaggg catttcattt tttccatgtt atctcaactt cctttcatct ttactctctt
    44161 agcttactct tatattgtaa aaaagagaag caatatcctt cttctatgca gatatccata
    44221 cctgacggtc ctgttctcca tctattaatc ggactttata ataaggacgt tcatctttga
    44281 ctagaagtgt ccactctttc acttttgctt ttttcccgta gtagctgact gcttccttca
    44341 tcgctgcttg cggttgtttg aactcttccg ttttttctat atcaaaaaga tgttctgcgg
    44401 ccttttccgt gtcatttcgt acggttgttt tcccagtgga tggattgact gctatgactt
    44461 gtccttcaca aaaaaactgg tattcatatg ttgcattcgt aggattctcc aaatcttttt
    44521 caactgcaaa atgaatagca gatatgttat gttcttttgt ttcgtttttt acgattgctg
    44581 ctgctttttt caatgagatc cgatattcct ggtcactaat atcatccatg tgaaaagcgg
    44641 cattgagcag tcccgtcata ccggtaatcc ctattacgat gagggcagca acaccaaaca
    44701 tcattttttt cattcttcat tctcctgttt ctttttttta aagaatatca agaaatgaac
    44761 gaatgaaaaa tcgaatgaaa cgaaaaaaaa gtgacaattt tgttatcttt attcgtgcag
    44821 ttctaatggc aatccgtctg gatcaaagaa aaacgtcatt ttttttcctg taaaggtatc
    44881 ttctctaatc ggttctgatt cgatccctaa ggactgcaat tctgcaattg tttcttcaat
    44941 atcgtctact ttgaaagcaa gatgacgcaa gccacaagct tctggatagt ttggtcgttg
    45001 tggcgcatta gcaattccaa aaatctccaa ttcagcactt cctagtttca gatctaattt
    45061 atagtcatct cgttctttgc ggtaattttc tctgattact tcaaatccta atttattaac
    45121 ataaaaatca cgtgatttct gataatctga aacaatgatg gctatatgat ggatcgttgt
    45181 aaggttcata tgagcactcc tattcttcgt ttaagctcgg atctatatta atctccagtt
    45241 ccattgtaaa tgtgtcagat tgaaaaaaat agctgttttt cctttttata agaaaaaagg
    45301 caaaaaaaac agaaagtagg tcctccaaga cactcttcct gttcccaata taataaatga
    45361 aagtaagaaa ttatattttc aagaaaacaa acagaaaatt atcataacac aaattattta
    45421 taattgaaag actagttttt tttcaagaaa agcatacaat agattgtgtt agtaaataca
    45481 gaacacacta catttagtgt cttaaggagt cctcatacat gaagtcatat tttcagtttt
    45541 tagctcatca gctaaaaggc tggccgcaac aaaattatta tcttttcttt ttcagtcttg
    45601 gctgccaggt catgactctt gtcaatagtc ccatcacttg gctgtccgtt attacattta
    45661 tcggtaccac gcttggtgta ttgtgtattt tatccatcaa tgcggcaaaa tcagtcaacg
    45721 gtattttagg tattttgtct gcgatttgtt ttattatcgt tgggttttca gcaaagaact
    45781 atttaagtat cggtgaacag attgcttata tgatcacatt ggatatcccc gtattgttaa
    45841 gtaaagaatg gaatcataat atggcttcaa aaatccgaaa attcactgga aaaagctggt
    45901 ttatcgcaat catcagtact ttccttgtct atcttgtttc aggattcctg attggtcgtt
    45961 atacagatga tcctcggccg tgggtagatg ccattgcttt ttctattagc ttgactgctg
    46021 gtatcatcag tttcttgcgt tacaacaacc aatattactg gtggttagct tcagggcttg
    46081 ctcaaatggt cctttggtat atttcattta cgcatggatc tgcaagtttg gcgatgttcg
    46141 tgaacagctc tgtttacttg ctgaatgatg tccttgcatt taccgtttca ccttggtata
    46201 accaaaaaga acgtgaacgt atgcaaaaaa tggaagaaga atactacaac gcacaacaac
    46261 acgcataaaa aaactgagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
    46321 cacgtataaa cggcggaatc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
    46381 tttgaaaagc aattttcgtg aatttcttct tatttcttgg agttaaacgc ttctgtccca
    46441 gcctctattt tttatagtaa ttccttcaga tactttcttg cctcatcatt gaaacgcagt
    46501 gattcttcta acttctgata atattccaac gcttgtttct gagcttcagt aaaacgataa
    46561 tgttttcctt tccatttgtc gattgtctct tgcacatttc cagattggat atctttcact
    46621 actagttgcc atttttcttt tgagactgta cggtctttct tatcagcaga ttgtatagct
    46681 tctcgttctt tacgcaatac ttttaaaaaa ccatttctga ctatccgagg aatgctgtcg
    46741 aagaaccctt ctgtaaaacc atagcgacaa tattgcttcc aaataaatag atagattttt
    46801 ggttggatga atcgttggtt tttcgcaaaa tattccatct gagcagtgac ttctggtgtt
    46861 cgttgacgtt ttgtctttct caacacggaa ataaatgttt caaaataagc attaggatgt
    46921 actggcactt cttcatatac gatggattgc caatctttac gtaaaaatgc gcgatagttg
    46981 atcaagaatt cttctacatc ctttactaat tcttctgcaa agtcgtcaaa tagttctttt
    47041 tttatctgtc gcttttttga ctgcccattg atgtgttcac ttattttctt gatttcattt
    47101 tccaatgctt gataatccgc tatgtaaatc ggcatttcta cttggcgaaa ttctgcgagc
    47161 cgctgagcta attttatatc cggtaaatat ggctgtttca tccgacgatt ttggtacaag
    47221 acaaaacagt atttttgcca tagtccttca ggaaaatcga tattcttctg cttaagccat
    47281 aataattcgt ctaaggccag atcaactttt gtcattccct gatggatcga agctgccact
    47341 gtcggagaga catgcaggtg atcggcaaag tggttgatgc ctagttttag gatctttccg
    47401 gtttttggcg atttaacgat gtattgcact tttaattctc taccacactc acaatacaat
    47461 ttttgctggc ttcgttttgt tcgaaaaaac ggattgatct tgaagtcaga aaacacccaa
    47521 tctgttgcag ctaaataatg ttcttccata aaacgagagc tgattagata tcggcggtgc
    47581 ttatcgacta gttcccgctg ttttttcgtt aattttgcat agatctccca acgttgactg
    47641 cttgtgtatt gtttagaaat tttgatggga tagcttttgc tgatttcttt tggaaaccat
    47701 tcttcaaaga cggattgcca ttcttccacg gctgtttctt tcctttctaa ttacaaaaag
    47761 ctggctacta acataacgtt agcaaccagc cagtgtttct tgctaaatta gtgagttgtt
    47821 ccggcatttc cggaaatatc ttctttattt tctactgctg cttcaactgc ataagtcaaa
    47881 gaagaagcaa tgtcttctaa agacatagaa ggcgtaccca ctggacgatc gatcacttga
    47941 tcgggtgaga atggcacgtg gatgaaccca cctcgaattg ccgggaattg tgtttcgatc
    48001 aaatgaagca aacgatacat gatatcattg cagacaaatg ttcctgctgt ataagagatg
    48061 tacgctggta gaccatgatc ttgtacattt tttgtcattg ctttgattgg caatgaggta
    48121 aagtaagcgg ctggaccgtt ttcttctaaa gccgtatcga atggctgatt tccttcatta
    48181 tcagggattc ttgcttctgc tagattgatt gcgacacgtt cgaacgagac tgctgaccgc
    48241 ccacctgctt gtccgacaca gatcacgaca tctggttggt gctgttggat ggcttctttg
    48301 actacttgac tgcttttttc gaatacggtc ggtacttcca ctttgatgat ttcagcaccg
    48361 ctgatttcat ctggcatttt ttttactgct tcataggctg gattcacttt gtccccacca
    48421 aatggatcaa aacctgttac tagtactttc atctgttgtt cctccgtccg attgatttaa
    48481 catatggtta tcttttatca taccattttc cttgaacagc gtaaataaag gaaacaatag
    48541 tttaaataac ataacagatc aaacttaata tattcttata aaaaaatagt gttccactac
    48601 aatgttagaa gaggttgcga caattattct gtcacaaccc ctctcttcta ttatttaaat
    48661 agaatcatat aagcaatttg gaaaactagc ataaataacg caataaacaa ctggtttttg
    48721 atcactccgt atttgtcttt catctccagc atcgctacag gcacgatatt gaagttagca
    48781 gccatcggtg tcagcagggt cccacaaaaa ccgcaagtca atgcaaccat tcctattaac
    48841 gctggattcg ctccatgact aaatacgaat ggtgcaccga tcccaaccgt catcacggtg
    48901 atggccgcaa atgcattccc catgattacc gtaaatatca ccatgccaag agcgtaaatg
    48961 atgatgccga tgttcacatt tccttctgga acgatcgtac ctactgcatt ggaaatcact
    49021 gtcccaactc cagcgcttgt aaataccgca cctagagaag ctaaaagcat tggcaacatg
    49081 cttaacggcc ctactgtgcc cagcatatct gctgcatcat ctaaaaatgt acttggtcga
    49141 ttatctcgag aaaagaacat caagattagc atggctgcaa aaacaccgac gcccacgccg
    49201 actaaagcac ctaaattggt aaatagagca aacaagatcg caaagacacc gatactcaaa
    49261 gctggaataa aaattttcat tcctagtttg tctgacattt tttccatata ttcttttgat
    49321 ggaagccggc tttctccttt gctgactttc ttagcaattg ctggtatggt catcgcaaag
    49381 atcaagccac cgttcacgat tgccggaagc catcttcctc ctgcaataac tacccctaag
    49441 accgtccaga aaaaggctga cccataacga tgaggattgg ttttatctgt taaatttctt
    49501 actccagtgt aaatcaatac gagtcccatc acgatataaa ggatctccag taatttatcg
    49561 cctaataaaa tatcaggatt tgtaaaaaag tccatcttat tgcccctttc ctggataata
    49621 tttcttcgct aatttctttt ctttaagata gaagtagaca cttgccacaa ctaaagccgt
    49681 gatagccact ggaacttcca ctaaagcaag ctgtcccaat gttacgtcat atcctagatc
    49741 tttcagtgtt gattggacta gcaacgcccc tgctcctccg atgaatagta cttggccaaa
    49801 gaaccagcag acattttcca tagctgaagc catacctttt agttcttctt catgcttagg
    49861 atggacttct aacccttttg attcgatggt ccccaatgcc atcggtaaaa tgattggacg
    49921 aacaaatcca gcaactccac caaaactcac gttaaaagcc gcaaagatca ttcgaaagat
    49981 cccataaata tctacaatca taccagcaga tactttcttg aatcgcttga tcaaggaagc
    50041 cgctgattct ttcagaccat ttctttcaag cgttcctgtt gccaacataa tgatgataaa
    50101 aatcgccatc ccgcgattgt ttacaaaact tgtacctaaa agttctaaca ttccttgaat
    50161 gccaagaccg ccagtcaaag ctgtaacaac taaagcaacc attacgatca atatcgagtc
    50221 taatttaaat gcaaacccga gaataacaat caagacgcct aaaaggctaa gataattttc
    50281 catatatttt cctccataaa atgacaatat ttttttaatt ataaagcaaa acacataaaa
    50341 aatatagttg ttttctcacg aaatagacac tatttttaaa tatttttcgt tattttttta
    50401 tcacaaaaat tatcttactt attgtaaggt aaagcttttt ttaatttatt gcccgataat
    50461 atatagaaat aaggtctagt tactgttttt tttaaatgat taataaataa caacttcttt
    50521 ctatgaaatt gcaaagcatt catcttcttt tccgctaacg atatatccgt cagtgcttgg
    50581 tacactttac taaaaagtat gagattgccg atatctggat aggtgtgcta tactacagaa
    50641 aaagatatac aatctgccac cgggtaggat aaagaggatg atcattccat taggtctttg
    50701 aagggatgat tttcctcttt ttgtgtattt ttttatagtt aaggaggaat gtaagtatgg
    50761 catggatgga attaatcata gcaggaattt tggaagtatt ctggtcaacg atgatgaaat
    50821 ggagtgatgg tttttctaaa atcaattatt ctttttacac agtgattggc atgattgcta
    50881 gtttttactt tttgtcaaag gcaatcaaaa gtttgccgat gagtctctct tatcctatct
    50941 ggacagggat tggcgcagtt ggttcggtca taattggtgt ggtcttgttt catgataagc
    51001 tgaatctttc cacttggttc tttgtgggga tgctactcat cagtatcatt ggcatcaaga
    51061 ttactagcgg ccactagaca cttggaataa gtactgatca gaaaaaataa gcctttttat
    51121 ttaatattca atttttttat gaaaaaataa gaaataagtc caatgaccta gcgacttctc
    51181 cactcatcag gtgtatgctt gtactatcta tagaaaagga gtgaatattt ttgaaaagtc
    51241 tttcggtcca agaattatcc aagaactttg gtcacaaaca agccctcagc gatgtcagtt
    51301 ttagcttatc ccctggcaaa atcgttggat tagtaggtcc taatggcgct ggaaaaacaa
    51361 cgatcatgaa agctatttta ggactgatcc attactcaga tggaacaatc tgtattggtg
    51421 aaaatgagat ttctccctct agtcataaag ggttggaaca agtaggagca ttgatcgaat
    51481 acccgggtat ctatccattc ttaacaggct atgatcatct gaagttattc tctgaaacca
    51541 acgatgtatc tgctatcgac actatcgtga atcagctcaa gatggaaaag tacattcaca
    51601 aaaaagcgaa atcctactcg ttgggaatga aacaaaaatt gggaattgct cttgccttac
    51661 tgaaccatcc gtctatcgtt attttagatg agccgatgaa cggtttggat ccacaagcaa
    51721 caagagatgt gcgaaaaatc attaccgact tggctactca gggcacctct ttcttgatct
    51781 ccagtcactt actcagcgag cttgaaaaaa tggtcgatga gctgatcctg atcaatcaag
    51841 gaaagatcat caaacaatgt acaatggctg aattgaacca aacagcgcaa gatttcatcg
    51901 ttatctctac aacaaatgat ctagctgctc gaacgatctt gacagaagct ggatatactt
    51961 tggaagatcc tgataaaatc aagcttttaa aagaaaacga agaattatta ggaaccgtga
    52021 tccagctatt gtctgcacaa aatatccatg tgacagacgt ccagcatctg acgaatgatt
    52081 tggaatcttc tctattggaa attctggatt cagtaaaggg ggaagaagaa aaatgacatt
    52141 gcttgtaaaa caagaattat ttaaattgat caagaaaaag tcaactgctg ttttatctgt
    52201 tctattagtt gttttgctca taggaacagc acttctagcc aaaaaataca cgacgatcat
    52261 tgatccagtt gaaatgactg ctcagctttt tagtgccaca tcttggattg tctttatcat
    52321 gattgcagct gcaagcacga tcatttcgat ggaagcacaa tatggtacat tgaaaaatct
    52381 cctttatcga aagtattctc gaggagaaat attagtaagc aaatggatca cattagttat
    52441 ttattctgtt tatttatacc ttttagctat tattgtcact gtgttgatga aactgattct
    52501 tttcccttcg atttccttca cggagaaggt ttctactggt caaacactga tccaaagctt
    52561 gttcacttat actttgggta gttatatcgg tttatggctg attttaagtt tagtgttgat
    52621 gattgcttgc tttatcaata gttccggcgc atccatctca gcaggaatcg tcttttattt
    52681 tgcttcgtca atcatatctg gtatccttat tgcactcatc caaaaatggg agtggatcaa
    52741 atggaatccg atcagtatgt tgaatctgca aaaccaaatt ggaaacgaag agatcatgaa
    52801 gcaattgact catctttcta ctaatcaaat gctattcggt aatttagctt acatcgtttt
    52861 gttcttagca ttaggatatc tcgtatttaa gcgaaaaaat gtatgatgac tcatcaaaaa
    52921 agaggctgtg aaagagacgt tcagccttat tgccgattgg ctgtctctta aacaccgttt
    52981 attctgaaaa aggagctgtg acaacatttg tgtcacagct cctttcctag tatgtttttg
    53041 aataaacgac aggctcacaa cctctcttta tacttctttt atacttctat acgtgaagca
    53101 tcccagtggc aaataaaacc attgcaatca ctccgaccat cgtcacacct ataagtaaaa
    53161 tcttatctaa gtggatatgt tgtacaggct cgttttgctc cttacgtgct ttgaggtaaa
    53221 aataaatgcc tggcaagaaa agaacaagaa caagcattac ttcattaaag gctacgacta
    53281 gcatagccca tacttgaaag gcacttgcta aaagccctac taaaaattgg acccattctt
    53341 tccgctgata agaaagtttc atttggtaca atcctaagaa aagccacgtg aagaaaatag
    53401 ctgctgtacc aagtgtgtaa gcaaagttat aagcactgtc agtgaataat aatgaaagta
    53461 aaaagacatt cgtacataac gtcgtcaata cgatagcata agttggtgcc ccatttttgt
    53521 tgagttttcc ccatgcttta ggcaaaagtc cgtcttcagc catgtttccc attgtttcaa
    53581 tcggtaagat cgtccatgag agccaaacac caactaaaga aatgatcaaa ccgccgttga
    53641 tcacgactgc tccccattta ccaaccatca tttcaaacac atagcccata gcaggctgcg
    53701 ccttgatcgt ttgcagctga ttcatcgtca ttgtcccata tggaagcatg gaaaccaaga
    53761 cgtaaataat aatcaagccg aatatcccta gaatcgttgc tttcccaaca tctgattgtt
    53821 tttttgctcg tgaacctacg acagaggctc cttcaatacc aacaaatacc caaagcatca
    53881 ccatgatgca actcaatact tgactggaaa acgggatcgg ctgtccattc gcattcaata
    53941 taatgtcgct agaaaagtta tcaatgaatt gttggatcga aaacgtgctg atagctacta
    54001 caataaataa aaataatggc actaatttac agaatgtgac gatagcattg acgaaactag
    54061 cattttcgac tccccgattc actagccatg catatcccca taaaaagact gtactcatca
    54121 cgatcgatgc cagattttgc ccaccttcaa atactgggaa gaaatatcct aatgcgctca
    54181 tcaacaatgt tgcaaaagct acgttgccta accagcatgt gagccagtaa ccccaaccgc
    54241 tgataaaacc agcgaaacgg ccaaaacctt cttctgcgta actaaagatc ccgcctttta
    54301 aatctggacg ttttttattg atattattga tcgacaagga gagcatcaac attgctgttc
    54361 ctacgagtac ccaactgaaa atagctgggc cgccagccga tccggagatg tccgtcataa
    54421 taccaaatac gccacctcca atcgctgaac caattactaa ggcagcaagt ggaattaaac
    54481 caattccttt tttctgttgc tctgtatcca tgtaagcatc ctccaaaata aaaccgcttt
    54541 tatcaaaaag tataacaaac cttttttatt tttacttaat taataaaaac tttaatagaa
    54601 atataattct accggctttt ttctcattat acaagcaaag cgcctagtcc ctttccaaat
    54661 tacaccacca aaaaacttaa aaaagatgct atactaaaag gaaagaattg gaggatgatc
    54721 catgaacatt ttatatatat cgatttcagg aaatacgcgt gcctttgcca aacatctagc
    54781 agaatatgct gaaaaaatgc atgcagaaga tcctgtcaat cctgaagtta ctttaaaaga
    54841 aatccatgaa aatagtgatt ttacgaaaga aacagaacca ttttttactt tcgttcctac
    54901 ttatttagac ggcggaaatg gtttagataa cggtgataca gaaatcttga ccgaaacgat
    54961 gcgtgaatat ctggaacatg aagacaatca taaactttgt cttggtgtag tcggtagcgg
    55021 aaataaaaac ttcaataatc agtattgtct aacagccaaa caatatgcac aaacatttgg
    55081 ctttcctttc ttggcagatt atgaattacg gggaacacca tctgatgtga cacgaattta
    55141 tcagatttta gcagaaaaca ataaataata aaaaagaaga agctgaaaaa tagatttcag
    55201 tttcttcttt cgtttctatt gtttctatca tattaagctt tgacatccat cacaggtgct
    55261 tttgcttttg ctttacctgt tttttccaag acaaagtttt ttacacgatc catattggtt
    55321 accaagacga tcatggatgt ttctttgcct gctgctttga tcgcttctaa gtcagcttct
    55381 gctacaagcg tatcaggtgt cacttgctgc ccttctgtta ctttcacatc aaatggcgta
    55441 ccgcctaagt caactgtgtt gattcccata tgaagcaaga tttctaaacc atttggcgtt
    55501 gtgatcccaa tagcgtgttt cgttgggaag acagagctga ctaccccagt aactggagaa
    55561 tagatatgtc cgttttctgg ttctacaaag tagccttcac ccatcatctt ttgtgcaaaa
    55621 actggatcac ttgctttatc taaagaaaca actgttccgt cagcaactga tgagaggata
    55681 gtcacacttt cagctgaagt ttgaggtact actttttcag gcacttcatt tactgtggct
    55741 gtttcagtcg ctaattcttc tttttctaaa cggtctttag gaataccgaa gaagaatgta
    55801 gcaacgaagc caccagcata agctgccaat aatccaagga tgtagcctaa ccatttaccg
    55861 tcagagatca atgggatcaa agcggctcct gaaggtccaa tagcgatagc cccgacattt
    55921 ccgatcattc cgatcactgc cccgccgata cctccaccga tacaagcagt aataaatggg
    55981 cgtcctagag gtaatgttac tccgtaaatc aatggttcgc cgattcctag gatcccgact
    56041 ggtaaagctc ctttgatcat ttctgttaat tctttatctt ttttgcagcg aatccataat
    56101 gccaatgctg cacccacttg tcctgcacca gccatcgcaa gaattggaag caatagcgta
    56161 cgaccagttt catcaatcat ttgcacatgg attggagtca aaatttgatg cagtccgaac
    56221 ataaccatcg gcaagaataa tgtgcctaga acgaagccgg caaatgctcc accaacgcca
    56281 agtacccagt tgatcccacc gaccatgcca tcagaaatga aacctgctaa aggcatgatg
    56341 aagaaaattt caatcagacc gatggcaatc aatgaaagag ttggtgtaac gatgatatct
    56401 accgcatcag gtactacttt atgcagtttt ttctcaacga ttgacaatag ccaaacagcg
    56461 aagatgacac ctaggatccc accttgtcca gctgcaagag cttcaccagt aaataagttt
    56521 ttcagtggtg cttccggatt catccctgtc agcatcacaa cggccccgat aactccacca
    56581 agagttggtg ttgcaccaaa aacttgtgca gcattgatac ctgtatagat aactaggtag
    56641 ctaaacatcc cgtttttcaa gatgttcatc acatcgatat attgttgcca tgttgctgca
    56701 tcaagtgttc cggctgtgac taagtttgct aaaactgcgg caatccctgc cacgatccca
    56761 gtaccgacaa atgcaggaat cattggcaca aagatattag aaatgtcttt taataccgat
    56821 ttgattttag atggtttttg tttcgatttt tgtgccgctt tcatttcttg tgcccgttcg
    56881 tttacacggt cttttccaga tgctgatgaa tttgctgtag ctgtacttac agaagaaagt
    56941 ttttctccta atttcacgcc tgccaaatca accatttgct gtgctacttt gttcactttc
    57001 ccaggtccga tgatgacttg aagctgttca tcttctacta caccaagcac tcctggaatc
    57061 gcttttaatt cagagacatt tacaagatca tcattgatga tcttcatacg cacacgagtc
    57121 atgcagtgga caatgctttc tacattgccc atgccgcctg cccctttata aatttgtttt
    57181 gccagttctt ccaaagtcag ttcgtttttt gccatggtct tttccccctc ataaggtccc
    57241 ttttacaaat ccgtttgctt cagctaattt tgcttgagct tcctctttat cactatttgt
    57301 caaaatcatg acgatagcca gcttaacatt ttgatctgct tcttcaaagt acttttcagc
    57361 tgtttcgtaa tcagcttctg tcgcttgcat gatgattcgt tttgagcgtt cgattagttt
    57421 ttcatttgtt ggtttgacat cgaccatcaa gttattgaat actttaccta taccaatcat
    57481 actgatagta gaaatcatat tcaagataag tttttgtgct gttcctgact tcaatcgagt
    57541 agagcctgtc aaaaattctg gacctgcatc gacttcgatt gggaaatccg cgtgtttact
    57601 gatttcggct tgtttattgc aagaaatcgt tcctgtgaca gcaccgatcg attttgcgta
    57661 atctaatcca ccgatcacat atggtgtacg accgctagct gcgataccaa taactatatc
    57721 attttttgtc aaattcagct tgattagatc ttcttgccct agctcataag aatcttcggc
    57781 cccttcgaca gctacagtca ttgcttcttc tccaccagca attagaccta tgaccatttc
    57841 cggttctaca ccgaatgtcg gtacgcattc agcagcatct aacacaccta gtcttccact
    57901 tgttccggct cccatataaa tcagacgtcc acctttttta aaggactcaa tcgtgttagc
    57961 aatcactggt tcgatacttg tcagttcttt ttcaaccgca gcggccactt tctgatcttc
    58021 tttgttcatt agttgaacgg cttgagaaat actcatctca tctaatccaa aagtttcttg
    58081 gtttctgcgt tccgttgtta ggttctctaa attgattttg ctcattttta tcttccttct
    58141 tttcttataa attgatagtt cataccagct ttgatttggt tgatcaatgg tagctcttct
    58201 ttgataactt tagctactac attcactttt tcatccgctg gcaaatcata tttggttaat
    58261 tggatctcac ccatatagcg caaatacttt tcgttatcta aagtaataga ccctttcata
    58321 cgcgtagctg tatttcttgc tgggataggc ggaatctctc gaaaacgtgc atctacgctt
    58381 cgtatgacat ctcttgcatc gtcttgccga ttcgtatgct tccctaatat atattcgtaa
    58441 aattcttcat ctacaggttc cgtatggagc aagactgtct gttctttttg gaaagaagca
    58501 aattgctcct gaacgttttc tgacagacct gcatctccga tgtaaacaat atccgtgtta
    58561 cttgctaata gatctagagc gcctgcaaga ggatggatcc ctcgatgttc ttccaaagtt
    58621 ggcaatcctt gatatagtgg gcctctcaac tctgtatctc ctggtacaaa accttggaca
    58681 aggagacctt gaattttgag ccattgattt tttttctgat accattcttt gtccagccct
    58741 gtttcgggac gtggataata attatgccaa gcttcaatct gtgagaaatc agcttctgct
    58801 tctttcaatt catcgatatc tttaggcgtg atcgtgctag cattcaaact gattttcaat
    58861 tgatgtgacc agtcggcaat ctgctgattg gaaatatgat aatccattct taaacctgtt
    58921 acaccaattg ctttcaaagg cgttagatct tctaatgaaa aacccgctcg ttccaatgcc
    58981 tctcctgaaa tatcgaccat caattctagt ttattctttt tcgcttcgct tcctagttcg
    59041 atcaatcgct tttgatatgc cgaaacatca tcttctggta tatgcatgga agtgaaaata
    59101 ccgacaaagc cattggatgc catttggcgg atatactgtt tcttctcttc tgacaaagaa
    59161 tcgttcataa atattgaaaa gccaaacata ttatccctct ttttctgtat ccgtcttcat
    59221 ttctatgact agagtatata tctaaagaaa caaaatttca atacttttat taaaataatt
    59281 aaatttcatt tctaaaggtt gagtttatat tatcaaaaga attgtcataa cctaaaaaag
    59341 gcactgctaa gcaaaagcag tgccttgaaa tattttattt aaataacaca aaaacataat
    59401 tactcataag aaaccaattc tgtattatct accatgaatg taaagaaccc ctcattaggc
    59461 atcggatgaa aataagcatt gatcactctt atcaaggcat tatggcaaac taacagatag
    59521 acattttctt cgtcttcgat gcattctttc aataaaggaa cgatccgtgc atacacatct
    59581 aagactgact ccccatttgg gaatcgtaca gcgaacgcta aacgtgcttt ttgaaagttc
    59641 tcatctttgc ttggcagtcc atcataatcc ccaaaatcca tttctaccaa tcgttcatca
    59701 acggtcagcg gcaagctgag acgatcagca actgcttgtg ctgtatctct tgctcgttga
    59761 agaggggaat ggatgatttt cgtgattgga acaggcaaat ctactacctt ttctgctagt
    59821 ttctgtgcct gcatctgtcc tttttcagtc agcggcacat ctgcccgtcc gcaaactaat
    59881 ccttgtgcgt tccaagttgt ttctccgtgt ctcgttacat aaagcatgtg attctctcct
    59941 tcgttggcaa agttcccctt aaccatacct tgttgttctt gagattacaa gggttagatt
    60001 ctctcaagag aaataatctc agtagatact ttatccagga aggtttgatc atgttccact
    60061 agtaacatgg caggtttcac gttcaagatc agttgttcta attgttcttg attgaagaca
    60121 tccaaataat tcaatggttc atcccatgta tatagttcag ctggctgtga taaagattta
    60181 gccaattcca cttttttccg ttggcccata ctcatctgct cgatcttgtt atgaaaaaca
    60241 tctctttcca tcccaagctt tcggaggttg ttcaaaaatg cttggtagtc tacttggttt
    60301 ttctctgcaa attccgccaa cgttcctcga ttgtcttcat aattttggct tgcataacta
    60361 atgctcagat gttttggctg gtatttttct cctataacct tgccgttgaa tgcccccaga
    60421 agataatgga taatggatga ctttcctgca ccgttaggac ctgaaatcgc cacccgctga
    60481 tgaggctcga ttgtaaaatg aattggctcg aataacagat tttcataccc taattgaaga
    60541 tcttctacgc ttaaaagtcg cttatggtga gacgcttggc tattcatcgt caaagaatcg
    60601 atatattcga tatcttttaa tagtttttct ttctcgctga tctgggtctc catccgattc
    60661 acgattgctt tggatcgttt catcgtccgt gcagcatcag cacccactgc tcctttattc
    60721 actcgtctag attcagtatc gatgaatccg acttgtttct ttgttttatc tccttctcgg
    60781 gaacgagacc attcggcttt ttcagctgct gttttcttta gcctactgac ttcttttttc
    60841 aatttttcat tttgagccat ttcaaattca tcacgaagtt ttttctgttc ttcatagata
    60901 gagaaattcc cttgataaag ttccagttga cttttttcga ttgctaaaac atggtccact
    60961 acttcatcga taaatccccg gtcatggctg accacgatga agccttgttt tttctttttc
    61021 aaataagccg ctacttgttt tctaccagag atatccaaat gattcgttgg ttcatcgatt
    61081 aacgggaaat gagtgtcatc cacaaataaa agtgccagta ggacttttgt cttctctccc
    61141 cccgatagtg tgctgaattc tctccataag atttcaggat ctgtttgcat caattggagc
    61201 tctctttcga tttcccatat ctcaaaatcc gtaatatcat ttaacacgta ataggttaaa
    61261 cgttctttat cttttgtctg ttgcgggaaa taggcaaatt cttgctgatg gattacttgc
    61321 ccttgataag gtagtttgtt ttgtagaata ttcagtaaag ttgtctttcc tcgaccgttt
    61381 cgtccgataa gtcctagttt ccattgtgtg tcaaaattta gatttgcttg ttcaaataat
    61441 aatgtgcctt ggctgtcgta gccgaatgtc agatttttta tttcgatttt agacattttc
    61501 catcacctcg taaaaaaaga ccagtcctat tttcagaact ggctaaagag gatgcaaggg
    61561 ttggatacac acaaaaagac ccatacatcg aaaaaaccaa gccaatcccg aaaatctgca
    61621 cactgcttcc ttgtctccac aaggtacagt ctgaatgcag ctttcatcaa tcgacttaat
    61681 ttttcaatgc acggatccct cactttgtcc tatatttgaa attacgatag cataaagaaa
    61741 atgattttgt caactacttc gggaggatgg tgaaaaatct gctaagtgtc tgtcctaaga
    61801 tttttgtttt ttctaatgtt tgcctgtgtt ccattgcttc ttctaaacct attggaccca
    61861 aatgaatact taagacaatt gggagaaaat cagaaagtcc ttctagctct tggtctctac
    61921 ttccgcaaat agcgattatt ggttttcgat atttttttgc aagcactgct acaccgtatg
    61981 gaacttttcc gtggatcgtt tgctgatcga tccgcccttc tcctgtaatg atgacatctg
    62041 cttgttcgat ctttttttcc aatccagtga tttggctgac taaatcgaaa ccagaaacca
    62101 tcttcgctcc caacaaaaaa agggctccac caataccgcc tgctgctcca cttcctggaa
    62161 tatcatctaa atccacctcc cagaaatctt ttatttgctg agcaatcttt cctgcttgtt
    62221 tatctaagaa agctagcgtc gtatctattc ctcctttttg tcttccaaat tgccaagcag
    62281 ctccttgttg acctgtatat ggattggtaa catctgcagc gataatcaat tttactttag
    62341 aaacaagaac gtttcttcga tcaaaatgaa gttttttagt agataataaa ggatttcctt
    62401 ctgtgtaccc ttctaaagaa gcacccaagg attggagtaa tcctagacca ccatcgctac
    62461 acccgcttcc tcctaaagtg acaataattt gttctacttc ttgaagcagt gcatctttaa
    62521 taagttctcc taaggcataa ctggaagcaa gtcggatagt ttgatccgtt ggtttatcaa
    62581 gtagatctaa ccccaatgct tgtgcagatt cgatgactgc ggtgtttttt ccttgccaag
    62641 ttgtcaataa atagctgatt tttcgctgac ggaataagag atccatccca tcaatggtac
    62701 gccatgttcc cctgagcgcg taatagattg cttctgcagt attttctccg ccatctgcaa
    62761 tcggcacatt gatttttctt cctttacgat cttcccagcc atttagaact gcctgattta
    62821 gttcaaatga agaagcactt cctttaaatg aatcaattgc cgccaaaata tccatctgta
    62881 tccctcactt tcttcttaag taagacactt tttttcattt ttttcaaaca aaacaatgct
    62941 attttcttcc atttttctat atactaaaag ggaactcatt gaataaagga gagaaaaaac
    63001 atgaaaatcg caatagctgg ggccggagca atgggcagcc gaatcggatt gatgctacat
    63061 caaagcggaa atgaggtcct cttgatcgat cgttggcctg ctcacattga agccattcgt
    63121 acaaatgggt tgattgctga ctttaacggt aaagaagtgg tcgcaaaact gccaatctat
    63181 tcaccagaag aaatcatcga aagcaacgaa cacgtggact tgatcgtagc attaacaaaa
    63241 gccaatcaat tagatgatat gttttgttcg atccaatcca ttattactga caatacttat
    63301 gttttgtgtt tactaaatgg attaggtcat gaagatgtac tggaaaaata tgtgccaaag
    63361 aaaaatatct tatttgggat cacgatgtgg acagctggtc tagctggtcc cggaaaagtc
    63421 acattgttag gtgatggaga aatcgaacta gagaacttgg aaccagaagg cgaagcattt
    63481 acaaaaaaag ttgtagaagt ctttcaagaa gcaaatttaa atccgatcta tagccataac
    63541 gttcgttatt ccatttggcg aaaagcttgt gtaaatggaa cattgaatgg tttatgcacg
    63601 attttggatt gtaatattgc tgaactaggc gcgcaaaaag cggcagagag catggttcga
    63661 acaatcgtat ctgaatttgc ttctatcgca gcaaaagaag gaatcatact agatcaagaa
    63721 gaagtgtacc aacatattgc ttctacttat gatcctgaca acatcgggtt gcattaccca
    63781 tctatgtacc aagacctgat caaaaaccat cgtttgactg aaatcgacta catcaatggt
    63841 gccatttggc gaaaaggaca aaaatatgat attgctacac catattgtgc ctttttgacc
    63901 caattagtcc atgcaaaaga aggaatcctt ggcgcagaat aaaaggctct ctgatgagaa
    63961 tcagctgaat cagcagctgg ttctcttttt ttaagcccaa taaaagatca aaaagaaaac
    64021 acaactctca tgatgccata agaattgtgc ttgtttgttt cagtacataa tctcttcaaa
    64081 atgtaggttt tttatttgtt ttcaacagag gagaggtcat attcgtattg ctcataaata
    64141 gtggaatcgt tagtgataaa tcgttttgcc caattttctg catcataaaa ttccaatagc
    64201 tctgatagga ttggcttaaa tggtgtatgc agaaattccg gtaattcatc taaatcacgt
    64261 tctcgaagca aaaaaatatt ttcaggacga taaaaatgat aatgagcaat cgcttgatta
    64321 tccgtcacta gtttcttttg aaaatacatg gattcaaatg tccgcaaagt cactccttgc
    64381 tgaccctccc gcaacagttc cagaatcccg cgcgattccc ctgttttttt caggtactct
    64441 tcataagtaa gatatccagg ttgttcattc cctcgatgtg gaagaatgaa atagttggtc
    64501 gtaacatctc tttttataaa ttcctgctta tagctttctg ctcgttcttt tctcccatta
    64561 ttcgttgcac caaaaaatag atccgtcttt ggttcgctag aagaaaaatt catatgtttc
    64621 gaataaaatg aactattatg tttcagccca agttcttttg cttccaaaaa atcaaaatga
    64681 taaatctcat cgattgccgg ttcatcttgt gcctgcttca gcatcgcctc gtgttctgct
    64741 ttgaagtgat tccaaaaaaa taacacaatt tttgcattgg taaatgggcg cacgcgtcga
    64801 gccatctcca tatcaaatcc ataatctaaa aaaacgatca gctgataatt tgccaactgt
    64861 tgtctagctg atttaatgaa ttccgactgt agtaaatctt ttccggacca aacatctaaa
    64921 gagcggatgg aaggcaaaaa ataaagtgat ttattttttg caactagcag tacgtcattt
    64981 ttgttcatct tttccgcctc actttctctg tcactttttc ctatgatagc ctgtttaaat
    65041 agtaaaaagc cagtaaaacg tttcctatat ttgtatgaaa atttaacatt tgtatgaaaa
    65101 tatgatgaaa taaatcattc ttctattaaa attcgcctaa aagttttctg aatagttgta
    65161 attgtctgaa aatttgttat cattagtcat tgttaaatat tgctatttat tcttaatata
    65221 aattttgatt gtgaatttaa aagaaagagg tcatttatgg aagataaaaa attgaaaaaa
    65281 caacttagct ccagacatat caccatgtta gctttaggtg gagcaatcgg tgcaggtcta
    65341 tttaaaggaa gtggcgaagc gattgggatc gccggccctt ctgtattgat tgcttttttg
    65401 attggtgggc tgattttata tatcgtgatg aaaggtctgg gaaaaatcgt tttaagcggt
    65461 ggcgacacgc atcacggatt atctggtttg atacgccctt atctaggtgc tcattctgct
    65521 gattttactg attgggtcta ctggtctatg tggattatca atatcatcgc tgaagctgtt
    65581 gcagctgctt cctttttgca gttgtggttc ccgcacattc cagcctgggt atttgttttt
    65641 atgttggctg ttttaactac gatcatcaat ctttattcgg tacggttatt cgcagaaaca
    65701 gaatattggc tggcttttgc taaaatcagt gtcattatct tactgatcat ttttgctact
    65761 tatcttgtcg gtcagcaaat gttgggcaca ggcgttttcc ctacccttca gcaattgact
    65821 gaccatggtg ggtttactcc tcatggtatg aagggaatca tcagttcttt gcttgtagtt
    65881 atctattctt acggcggttc tgaactgatt gctatcacgg taagcgaagc agatgatcct
    65941 aaacgtgcga ttcctaaagc aatcaaaggt gtcatgggaa gaatcatttc cttttacatc
    66001 attcctttgt ttttgctttt gattcttttt ccttggaata ctttagcagg cacgaatgtc
    66061 agtccttttg tgatggtatt tgaaaaaatg aatatcccat ttgcagcgga tatcgtcaat
    66121 tttgtgattg tccttgcttt gttctcatct atcaattcgg gtgtgtatgc atcttcaaga
    66181 attttgttct tccgtttgaa ggatcgaaaa ggttcctcta gaaaactagc tgttttaaac
    66241 aagcatcaag taccgcaacg tgctgtttta ttttgtacca gtgttcttta tcttggtgta
    66301 gctctttctt attttgtcgg tgacaaatta tttggctatc tggcaggttc cctttcgtat
    66361 acagtattat tgatctggat cattatcagt gcggcatcct ttgtactggc actaagaaaa
    66421 ggaacattat gggataaagg aatcagcttt tttgcattag ctgtcttagg attgattttc
    66481 cttggtatcc tgttcacgaa ctccattggg gtaactgtat tgactggcct gctttacctg
    66541 ttcatttatt tcagttatca aaaaaagaat gatgcgttcg tattgacgaa tgaatgaaat
    66601 cgatagaaaa ttttatttta tttatgtgga atagatggag ctattgaaga aaggagttgt
    66661 gacaaaagtc ttgttgcaac tcctttttcc gaataaacag tctcatgacc tcttggtaaa
    66721 cggtgttcaa aaaccctctg tgcaaaataa ttgattgttc tccatgctca tgacatgata
    66781 gaagaaagga atgaaaaaaa ggagtttgtt ttaaatgaaa aagtattgga agtttttgtt
    66841 gatgatcggt gtttcaacgg tagttatgtt tggtatgatg tacttgaatg tctatgcgct
    66901 cgatcatatc ttcttcagtc gtacacgagt attcatggcg ttgatgatgg gtgcagtcat
    66961 ggcagtcatc atgctgttgt tcatgtggaa aatgtatgaa aacaaaaaga taaatgttat
    67021 gatcttagcc gctagtcttc ttgtttttgc cggttccttg tttatggtac gaagccaaac
    67081 tactgtcaac gacacagctt ggatgaaggc catgattcct catcattcta tcgctatcct
    67141 ggcaagtaaa cgagctgatt taaaagatcc tgaagtaaaa aaattagcgg aagaaatcat
    67201 tcaggcacaa gaagaagaga tcgacaaaat gaaaaaaatg atcaaagaaa tggaatgacc
    67261 attatccgtc caaataaaaa aagaggatgt tccagaagtt atcagcttca agaaataaga
    67321 agaagctact tctggaacgc cgtgaataag gggttgtgaa aaagctgccc tgcatcaagt
    67381 aataagaaca gccaaacaaa aatagcttct cctatttttc gtttggttag gcttattacc
    67441 gcagatgcaa gcttttgaac accgtttatt aagaggttgt aaaatcagcg ttctgacctg
    67501 agtattagag agacaaatgg aaaaatagtt cctcatattt tgccattttt tgagctaata
    67561 ccgagggtca gctgattgaa caccgtttat cgagaggttg tgaaaaagtc gcttagtttc
    67621 gcgtattaag aaaaattttg tgaaatggct ttccacattt ttgtataaat ttggcttaat
    67681 accgagaaac tggcttttga ataccgttta ttcgtaaaag ggattgtgac aaaagtcttg
    67741 tcacaatccc tttttatgtt tttattgttc taattgcgct gtcttgctta ctgcttcttg
    67801 cggtttcaca aatttttttg agattcccca acctgtgaag gcactgacca tcgaaaacac
    67861 aggagaaagc aaactgaaaa atacgaatgg caagtaagtg agtgtaggaa taccaaatgt
    67921 acttgctacg aaacttccgg caattcccca aggcactaag tagttgatta ctgttccacc
    67981 atcttctagc gttctaccta atacagttgg atcaaatcct gctttttcat aagtttcttt
    68041 aaatgcatta ccaggcagaa taacagatag gaattgttca ccaataaaaa tatttactcc
    68101 gatacttgtc atcaatgttg taaaaatcaa atttggtgtg ttcgtcatct ttcttgatac
    68161 tcgttccatc acggttgaga tcaagccaaa ttcgatcaac aatccaccta atgacaaagt
    68221 tagaataatc aatgacacag taggcatcat actttcgatt cctccgcggc taagcagtga
    68281 atcgatttgt tggtttccag tatttgaaac aaaccctgat tcgatgaccc cagccaactt
    68341 cgttaaggaa gtttgtgggt tttgaataaa aatcattcct actgctacag cgatattcaa
    68401 caaaatcgtt aaaattgccg gcaccttttt ccatgcacac agtaacatta aaaataaagg
    68461 aatcagtgac cagattgaaa tagtaaagtt tgcttctaga acatcagtca cttcacgaat
    68521 agatgaaaga cttgcggctt tacttgtgtg tcctaaaatc atgaataaaa agaaagaaac
    68581 aacaaatgcc gggacagttg accacatcat atgcttgatg tgtttgaaca aatcggcatc
    68641 aacaatagct gctgctagat ttgttgattc ggataatgga gacatcttat ctccaaaaac
    68701 ggcacctgaa acaatagctc caacaaccag cgctggattt aagcctagtg ttgtcccaat
    68761 accgaaaaat gcaatcccaa tcgttgacat aacagtaaag gcactcccta cagcgctacc
    68821 tacgattgca cacacaacaa atacagaagg gacaaaccac tggacactga tcaatttgaa
    68881 cccaatgacc attagagttg gaatgatccc tgcttgtatc catacagcaa tcaatgaacc
    68941 gactaaaata aaaataaaga ttgggatgat tcctggtttg atgccattga tgattccttc
    69001 gtgcagccaa tctgtcgggt atctttttat cactgcatac aacatgacca ttccgatcgc
    69061 tgctaagatc gtgatattag gtgagacctt taatccgaca acgccaatcg caatgcctaa
    69121 aacaataacc attaatacaa gtgttgcttc taaaaaactt ggtgttcctt tttttcccat
    69181 gataatctct ccttttttag ttgttcttat gttctaaaaa agttaagata tcggattact
    69241 ccgccgcaat tcatccgcga ttcctccttt ataaaaataa aaagacctgt tttaaactaa
    69301 ggacgaatga ctccgcggta ccaccttaat taaaaacaga tctgtttttc actttcaaac
    69361 ttttattcaa atgtatggac tttccatgta attcgtcaat taggcttgat cggttcacag
    69421 cgaccaccga ctttctgaac aaaacaccta accgctactg cgtgaaaagt tgacttgtac
    69481 gaagtttacc ttccataaga tcatttgtca atgaaaaaga aaatattttt tcatttttct
    69541 gaaaattatt tttataactt tcacacacaa gtttattttt tactagaaag tagcgccctc
    69601 ttgttttttt cttgctacta tttcttgatg ttcttgcttg attctttcta atagctttgg
    69661 ctctttcaac agatcaagag ctgtattggc gagtagttct gctgcaatcg caatagaagc
    69721 aaggcctttg tcacttttag cagcagcttt gaattcttct gtgtgtccgg caatatatgt
    69781 ctcagagatt gctaccgtag gctggattgt tggtatgact tggctgacat ttcctacatc
    69841 agaagaaccg agacttgttc tttgttccgt ttctatttct tcatctggaa cacctgcttc
    69901 ttttgcatgc tggaagaata agtcatcaaa agaaggagta acgatgacat catcaactgc
    69961 attttggaac aatccaaact caaaagtcgt gcccgtactt atcgcagcac ctcgtacgat
    70021 attttccact ttttcatata ctttatctaa tgtttgccga tttcctgcac gtaaatagaa
    70081 acgtccagca gcgtaatctg gaatgacgtt ggcagctact cctccgtctg taatgatccc
    70141 atggatattg acatcttttg gcaattgcaa gcgtagagcg ttgattccat taaacacttg
    70201 gatcaatgct tccaaagcat ttactccttt ttcaggtgct gctgctgcat gggaagcgac
    70261 accgtaaaat ttgatgtcga ctggatcatt cgctaaagat tctgtcgttt tcccataaag
    70321 aaaagctgga tggacgcata acgctacatc gacatcctca aaaaaacctt ctcgaacaaa
    70381 gctgccttta gctgatccgt tctcgccccc ttcttcccct ggggtgccat atacacggat
    70441 ttctccacca gtttcatcaa tgaattgctt cactatgctg gcggctaata cggagtaagt
    70501 accaaacaaa ttatgcccgc atgcatgacc aatacctggt aaagcatcga attctgccaa
    70561 aaaggcaatt gtgggaccgg gtttattgga aatataacgt gcatcaaagg ctgttcggtg
    70621 tccagctact tctttttcta cccaaaacct ttctttttct aattgtttaa tcaacgctgc
    70681 tgaggaataa aactcatagt tacttacttc aggatgttcg tggatatcta gagcaagtgc
    70741 ttgatattct ggtaaacgat attggattag atccttcaat tggtttgttg agattgttgt
    70801 tgtcataaaa aattcctcct atgctagtca tttttcttat tttctcccga atgtttccca
    70861 tgcaggcacg cttgatcctt ttgatgtttt ctcgattact ttgactgtgt cttctgtttg
    70921 ataagcgtct actatttttt gatacgtttt attgttttca tcttcttttc ttgcaacgat
    70981 gatattcaca taaggacgtg catggtcatc cactggttct aagaagatgg catcagaagc
    71041 aggattaaat ccagcatcta cagctacacc gctattgatc actgaaatgt ctacatctga
    71101 caaagcacgt gctgtttggg aagcatctaa ttcagaaatg tctagatttt ttctattctc
    71161 ggtaatatca gaaacagtgg gcgcttgtcc ttttgcctta tccactttga tcaatccagc
    71221 agtttgcaat aacagaagag ctctgccgcc atttgtaaca tcattaggga ttgctacttt
    71281 tgctccgtct ttgatatcag ctacatcctt caaagtgtta gaataaattc ccagcggtgc
    71341 attgacagta tttccgatcg atactaaatc agttccgtgt tctttattgt aattgtctaa
    71401 aaagaattga tgttggaaag aattcaaatc aatcgatcca tctgctaaag ccacatttgg
    71461 ttgattgtaa tctgtaaatt tcacgtattc caaattaatg ttctcctttt tcaaacggtc
    71521 tttcacgttg tcccatacat ctgtatcttc tccaataatc cctagtttta cgatagtttc
    71581 ttttttgtca gttgtatctg cttttccttg gctacaccct gccaaccaaa taattgctac
    71641 aaatgtaatg actacaaaac gaataatttt tttcattttt tctcctccac tttcaatgtg
    71701 taattttttt aatcagttga ttactgataa attgacttaa aaaaactaag attagaatca
    71761 ataatgtagc cacaatcgtt acatctgttt ggaatcgatt ataccctctc gatatagcaa
    71821 gattccctaa tccgcctgca cctactgctc cagccatggt agtcagtccg atcacgttga
    71881 tgatagtcaa cgccgagacc cgaatgatac caggcaaacc ttctaacaaa tacacacgta
    71941 aaatgatacc gattggactg gttcccatcg cttctgccgc ttcgatcacc ccaggatcaa
    72001 cttctagcag tgcgttctcg atttgacgag caaaaaacgg aacaactcca aaaatcaatg
    72061 gcactaaagc tgctttcgca ccaatcgtcg ttccaactaa gaaacgagta gtcaatgcta
    72121 aaagagaaag caagataatg aaagggatgg aacgaatgat attgatcagc ttatcaagta
    72181 tttcaaatag cagacggtta gctagtaatc cattagggcg tgtcactact aacgtcacac
    72241 ctaagatgat tccaatcatc ccgccaatga atgctgtcca aaaagacata tatagtgttt
    72301 gcttcgtcgc ttcgacaaat tcatccggaa tcaaataaac attgggcaaa tatgttttaa
    72361 agaaatcagt cattttatct tcctccttcg atcactttga tacgactatc taaatcgttt
    72421 ttttgtaaaa tatttatttt tacaccagat ttgatcaaat aatccaatgc ttttttccgc
    72481 tgtttggttt ctccagctaa agtaacgatc aaactcccta acggcgtttc ttgtaataac
    72541 tctacattgc catataaaat attcgctgtt acttgatatt tgctgtacag atgagcgatt
    72601 agaggttcgt tcgtctgatt tccaatataa cttaattcca ccagccattt attggcgatc
    72661 tgatcggcaa aagcagaatg ggcaatgatc gtttctaatg cttggtcaag atgagtagct
    72721 gttcgaataa aatctttggt caattcttct tcaggattgc tgaagatctg gatgctgcta
    72781 cctttttcaa cgattcttcc atcttccata accgctacct tgttgcagat ttctttgaca
    72841 acttgcatct catgagtgat caggacgatt gttaaaccta actggcgatt caattttttt
    72901 aacaatgcaa ggatctgaag ggtcgttttc ggatcaagtg cactggttgc ttcatcacat
    72961 aataatacct taggttcatt cgccaaagct cgtgcgattg ctactcgttg tttttgtccc
    73021 cccgacagtt gtttaggata agcagatgct ttttcttcca aaccgacaag ttccagtagt
    73081 tcattcactt tttgacgacg ttcctgtttc gattttccag aatactttaa agaaaaatca
    73141 acgttgtcaa aaatagttcg gctttccatt agattgaaat gctgaaagat cataccgatg
    73201 gcctttcttt cttttcgcaa ttctttggga gagagctgac tcagattcgt ctgattgaca
    73261 atgacttctc ctgctgttgg tttctgcagc aaattcatca cacgaactaa cgtacttttc
    73321 cctgcacctg agtaaccgac gattccataa acatcgcctt tttcaatcgt taaatctaca
    73381 gaatctactg cctgtatctc tttttctttt tgtttgaaac atacagaaac atctcgcaat
    73441 tcaatcatta aaattcctcc ttatctaaaa aaagcgtcct caagaaaatg aaaatgtcat
    73501 tttcttgagg acgctttttt gcgtgttacc acctcatgtt caagctgtgt tcacacacaa
    73561 ctcctcttca agtacgatca tactctagca agatatcggt cgcagccgtc atcgccttac
    73621 atctatgctc gggatgaatt gctcgatggc cattttccaa tagtcgttcc ttgcttcttc
    73681 tcagctaccg aagctctctg tcaaaggttc ttctatcgta cttaccattt caatgcaaat
    73741 aatattcaaa aaaaaagtcc ttttactaac ataaactatg tcagtaaaag gacgacgtat
    73801 cgcggtacca ccttttttcg gaagtttcct tccctcaagc ccaacatctc taaaagagag
    73861 attggcgttc gttttaacgg tcgaacagtt ccgaatttgc ctacttatcg acaaattatt
    73921 ctccaaggcc attttcagct tttcttactt gactcttctc accagccaga gcctctctgc
    73981 aaagtttcca aacttacttt ccttttcaac gaaatcgata ttttgttgtt gggattaatc
    74041 tacatgagga aaactgaaaa gtcaagtacg aaattcaaat tattttttct atatttattt
    74101 ttctgttttt ttatttaaca tttcaataac agttcgaaga tcttttcctt ccgcaagaag
    74161 tttcaatttt tccttttctt cgacggtata atttcggaca aaactggaat caggtatcaa
    74221 aacaacgcct aatcctgctc gtgaagcagc tgttgctcct actaaagaat cttccactgc
    74281 aaaggctgaa ctagcttcta atcctgttcg ttccaaagct gtcagataag gaatcggtga
    74341 aggcttgttc tccgacactt cgtctccgaa tgtaaacgtg tccaagtaat cagctaattt
    74401 aaagtgatcc aagatatctg ttgctctttt cgtaacagtg gtcgtagcaa tgcctagttt
    74461 catatgacgt tcttttaagt ctgccaatat ttcttttgca tatggtttta agtggatggc
    74521 tccgcttgtc agctgttgat aaaaatattc ttcacgcttc gctcggactt cctgtacttt
    74581 ttcagggcta cctgctgctt tcactaaaat atcaaaactt tgtttccagt tttttcctcc
    74641 ccaagcgtca acgatttctt ttgggatatg gatatcaaat tgctgaaatc cccaaagcca
    74701 gccatcacga tacgtttttt ctgtgtcaac taacaaacca tctaaatcaa atattgcacc
    74761 tttcatctat acattaatcc tttccaaaac aatacacata gtttagcacg actggtccaa
    74821 aaagaacaga agcaaagaac cagccttctc gtttataaga gttcttggaa tcgacggata
    74881 tagattttct tcacagctgt cacaagcgcc agataagcga tgactgtagc aaataaccat
    74941 aaccagtaat tgccaggaag tggcatcaag cctaattcag caccaaaact tgtaaaagga
    75001 aggacagaac cgacaataat tcctaatgta gtgataacaa acatggcaaa tgttgcgttg
    75061 ctttggataa atggtacttt cggtgtacgc agtgcatgga gcactaaggt ttgtgtccat
    75121 agtgattcga caaaccatcc tgcatggaat aatgcgatga aaatcactcg ctgtgaacta
    75181 tccaatgtat ggaaacttcc gcccactaca gcaggacaaa taacaaaata catcaacaag
    75241 taagtagtaa tatcaaatat cgaacttgtt ggaccaaagt aaaccatgaa ttccccaata
    75301 ctagatgcat tccatttttt tggctcgtcc aaatattctt tatccatccg atcccatggc
    75361 aaggaaatgc aggaaacatc atagatcaga ttcaaaaaca aaagttgtaa aggcagcatt
    75421 ggtaaaaatg gcaggaacgt acttgcaatc agcacggaaa acatattgcc aaaattagaa
    75481 cttgctgtag ccttgatata tttcatgata ttcccaaaag tttctcgtcc agaaagaagg
    75541 cctctttcca aaatcgtcaa atctttttct aaaaggatca cgtctgcaga ttctttcgca
    75601 atatccacag ctgtatctac agaaataccg atatcagcca ctttcattgc aggagcgtca
    75661 ttgataccat ctcctaaaaa tccgacagta tgaccatttt tacgtaaagc tgtcgtcaaa
    75721 cgtgcttttt gctgaggatt caattttacg aatacttcat ggtcttcagc tacttgtgct
    75781 aattcatgat cactcatttc cgagattttc tcaccggtaa ccaattcatt gatctctaga
    75841 cctacttgac ggcacactga acgtgtaact aattcgttgt ccccagtcag aacttttaca
    75901 gctacaccat gttctttcag agctttcaac gcttgtttcg ttgtttcttt tggtggatca
    75961 aggaaagcaa gataaccaat caaaaccatg tctgactcat ctttgactga aaattctcca
    76021 acgacgcttg gattggtctt ttgtgcgaca ccaatcactc gtaagccatc ttcattaagt
    76081 gactgtactt ttgcaagtac ggcttctttt cgttcttttg ttaatggtgt aacagtaccg
    76141 ccaagatcaa taaatttaga aatgttcagc atttcttcaa tcgcgccttt tgtgatcatt
    76201 tgtgttttac cagctggatc agcaaccaca acactcatac gacgacgttc aaaatcaaaa
    76261 ggaatttcat ccactttttg ataattgatc ttatctgtat ctaaagtttc tgttgcttca
    76321 tcaatgatag ccacatctaa caaatttttc agccctgttt gatagtagct atttagatac
    76381 gcatggcgca aaacacgatc atcttcattt ccttcacaat ccatatgata ttcaagaatg
    76441 attttatctt gtgtcaatgt ccccgtttta tccgtacata acacatcgat tgcaccaaag
    76501 ttctggatgg cattcaaatt tttgatcact gttccttttt tggccatcac acttgctcct
    76561 ttgaccaaat tcgtcgtaac gatcattgga agcatttcag gtgttagacc aactgctaca
    76621 gatagtccaa aaagaaaagc ttctagccaa tccccttttg taagcccatt gatgacgata
    76681 actgctggag ccatcaatga cataaatttg atcagtagca tggatgtttt atggatgcct
    76741 aattcaaagc ttgattgaat tggtttttca gatagcgttt ctgctacatg accaaatagt
    76801 gtttcatttc caacagaaac aatgattcct tccgcacttc cactgatgac attacttccc
    76861 ataaagacaa gattttcgta acttgtttcg ctttgctgat cgtcttcaca tactttcgca
    76921 tgcttttcta ctggatagct ttcccctgtc aatgctgctt gggaaatgaa taaatccttt
    76981 gcggacaata aacgcatatc cgctggtatc atatcccctg ctgaaagccg aacaagatct
    77041 ccacagacga tttcttccat aggaatctct gtgtactttc cattacgttt tacagctgcc
    77101 gttactttca caagtgattt cagtttttca gccgcttgat tggaacgtac ggactgaatc
    77161 aacgtcattg tcccgctgac gataaccata gtgaagataa tggctacacc gaaaagatct
    77221 ttatctccag gagctgctaa tacatattcc gtgatgaatg aaataattcc taatgcgatc
    77281 aacactaatg taaatggcgt gatgtatgct ttcaatattt ctacaatgaa tggtgttttt
    77341 ttgccatatg aaatcgcatt ttcaccgtaa ttttctcttg aacgttcgat ttgttcttct
    77401 gtcaatccat ttgctgatgc atggaatagt tcaaataatt cttgtttcgt ttttccagca
    77461 aaagctgcat agattttttg tgtatctttc tttttttcca tctcaatctc tcctttgtgt
    77521 ccaaaagaca gctgttctcg ctgcttttgg tctttagcta ttcatcggat cgatagagaa
    77581 aaaactgaaa ggggctgaga acagatagat aatagccctg gcctttcctc tagatccttg
    77641 tttttcgaat gactgtcatc catttgttct catctccttt actaagtaat tttaattcat
    77701 gacttccaat catttttcga atagttattt ttccaattag accacctcct ttttattttt
    77761 caccgatggg gatttgcggc aacaaaaaaa cctctcgcca aatactcagc gaaaggtttg
    77821 aaaaagcata ttaaaaaaag cttctcagat ctcatcgttg agttttgaca ctgtacaacg
    77881 taaaaagcag atacctgctt ttagctacct tatgaaaagc cttatctcga caattcctgt
    77941 tctacccatt ggcgtctctc gacgtttttg ggcagtagcc tatgtttgta taggagcctc
    78001 acctaacaaa tcttattcgg ttttccttcg ttaatataac aactaaaaaa gaggatgtca
    78061 aatcaatttt ccatgttttt catacttttt ttaaaataca cacgttatat tcctgtctca
    78121 cgcggtttta tttgtctcat tttccacctc ccgctataat agaaatatag taggaaacga
    78181 ggtgctgtta tggaaagaac acttgttatt atcaagccag acggcgtgag acgccattta
    78241 gtcggaagca tcatccaacg ttttgaagca aagggactgg ccattgctga aatgaagttt
    78301 gaaactatga ctcctgaatt agcaaaagaa cattatcagc acttgactga gcgcagtttt
    78361 tttgatgaac tgatcgatta catgacttcc ggtcctgtcg tttatcttgt gctggtcggc
    78421 gaggaagtga tcgatatcgt ccgtaagatg gttggagcaa caaaagctgc cgatgctgtt
    78481 ccaggtacga tccgaggaga ctacgcgctg ccggggactg aaaacatcat tcacgcttct
    78541 gattcccgtg atgctgcggt aaaagagatc gcccgctttt tcccgccatt cgatataaac
    78601 aaaccgtctg aaaaagcggc gaagtgatac aaattctaaa ctgctggtct agttgctggg
    78661 ttcagcaatg gccagcagtt ttttctctaa ggacctccgt tgattctttc ctaacatata
    78721 ttcgagcact gtgaagaatt cttggtagtc tttcgtcttt tcttccccta aagcagcaga
    78781 aatcaatctt tgataataat ctgccatcaa tgtttcatag atcactgtac ttctttcttt
    78841 atgtgctaag ctccgtttca tatcttcatt cacactacgt gcctttttca accgaccatt
    78901 ttctatataa aacgaagtca ggttcattaa aaaagtaaag agatactgct catgattgtt
    78961 caactgtgct aatcgtcgca ttcttttttt gattttcaaa taattgttct caatgaaccg
    79021 atcactgaat atccaaggaa aatcagctag aatagatagt tcaaattgac catagtctgg
    79081 tatctgcatc aagtattctt caatgattcg gcattcataa cgtaaaaatt caaattctgt
    79141 ctcagatact tctgtttttt cgaccatgtg aagattcaaa accaaataat acagatcttc
    79201 tgttttctta aaatgcctac ggttattgct gatctcttta taaggatcaa agctgtcttc
    79261 ttgtacccga tctagcattc gtcttttttt ctttaacttt attggttcat ccagctggtt
    79321 tcgcatccat tcatattctg caagtgtcat gttcattcgc tctaaaaatg atctcaatgt
    79381 tacaaatgac atttttttca ctgaatttgt ctcaatcccg actagagaag tcttaaaaca
    79441 gattccttcc gctaattggg actgtgtcaa cttcttgttg actcgaagtt tcttgatcaa
    79501 ttcaccatca tacataatag ttctcctttt taatttaaat ttttccacat tcaataaata
    79561 gatacgttaa aaaggaaaaa tacacgaaaa aaataaaaaa atgttttttt cataaaaaat
    79621 accaaccttc aagcattaga atgttgtcta actactgaag gttggtttga ttgtttattg
    79681 attatttttt tgctgttcaa cgtctgttct tgttactgga tataagttgg ttgttgtttt
    79741 attaccattt tttgaaaaga cacttgtcga atgttcgcct ttttgctttt cgattgcttc
    79801 attacgttcc gcttcatttt tatagtcata ttttttcgga tcaatcgtct caaatccgtt
    79861 tggcacataa aaccgcaaga gattttcctg attcagctta tctgaaagac ttaacgcagt
    79921 cttgactttt tcctgatctt tggagatttc ctcatcgatt cctgctgctt tcgtatcgat
    79981 gatttctcct gtctgattat tgtaagcttt tcctcctagg acggtgtatt tgggtgtcac
    80041 aaagttccca tttcgaaaag caactacttg atcatgctga gaagataata agtctgtacc
    80101 aaaatgaaga taatctttat catcgattcc caatagatgt agtaatgtcg gcaaaacatc
    80161 gatttctcca ccgtattcat gattgactgt ccctttagta taaccaggca tatggatcat
    80221 caatggcact cgttgcatct gtgcgttgtc aaattcatcc catgtatctg gatctttacc
    80281 aagagcgctt gctaaatctt tattatctgt attcgagatc ccaaaatgat ccccgtaaat
    80341 cacaaagatt gaattttggt aaataccaga ttttttcaaa tacgtaaaaa attgttccaa
    80401 tgattgatcc agataatgtg ccgttcgaac ataattatct actgtgctat tgccagtatt
    80461 caaagatgga aagtcgaaat tcttgtcatc cgtataataa ggcgtgtggt tggttacgga
    80521 aagaaatttc gtgtaaaacg gctgttgtag atgctcaaga tattgggcag attctctgaa
    80581 taaatcttta tctaatatcc cataacctaa cgtttcgtcg gattcatcaa aataagatcg
    80641 atcaaagaaa ttgtcataac ctaagttttt atatacatga tctctattcc aaaaactagc
    80701 tacattccca tggaaaacag cgcttgtata gccttgcttt tgtccaagga tcgctggtgc
    80761 ggcttgaaag acattgtctg acccaagttc tgtgaataat gagccttgag gtaaaccaaa
    80821 tgtgccagtt tccaacatat tttcagcatc acttgtcttt ccctgtccta cttcatgaaa
    80881 gaaattgtca aagctgatcg ttgcttgatt ctggaaaata ctatttaaaa acggtgtgac
    80941 ttcttgtccg tctaccttca tattaatcaa aaactgctgg aaactttcta aatggagaac
    81001 gatcacattt ttccctttag cgatcccaaa tgtttcggga tttggctctg cataatgttt
    81061 ctttgtataa tctaaaactt catcaatccc attactgctt gcatgcgcgc gaacactgct
    81121 agtcattcct gttttgattc cgtcataaat agtatacgca tcgattccta gatatttgac
    81181 aatatatgaa cggtcaaatg ttcgctgcag caattgtgga cggttggctt cgctcagcgc
    81241 tagatttcct gaaaaaataa gccctgaaag acaggtaaca gcgatagcca tcggtttgcc
    81301 gacagggttg cttttgatag gtattttctt gaccttcaac caaaccagta gcccaatgaa
    81361 cacagcgata tccagccagt agatgacatc atggggcttc atcaaagaaa aggagcttcc
    81421 tgacaatcct tgcgacactt tcgaaaagcc taatacagat ttgactgtaa tgaaatctgt
    81481 gaattcccga taaaaaataa tattcaagta caacaaaagc gtatttaaaa tatcaatgat
    81541 caaaagaata gctaacgctg gctttggctt tttaatatac aaagaaaggc caaaaaacaa
    81601 taaggtagtc gcaattgggt tgatccacaa gataaagtac tggatcgtac cttccacacc
    81661 taatgaaaaa tctgcatagt aggcaatgat cgtcttcagc cagaaaaaga aaacagctgt
    81721 caaaacgatt gctaaacgat tttggcacaa ttttttaagt tgtgtcacgt atatcacttc
    81781 tctctattca cattttttct tattttgctc ctgaaaaatg aaaatttcct aaaaaagttg
    81841 ttatcttaag atttttttat tctttctaaa gattattccg tccacttttc acagcataaa
    81901 accgctgcga tgtaagcgat taaactaatg aagagataaa agtcttgtgg tgaccgcttc
    81961 actccacggt attcaaaagc cagtttctcg gtattcgtac aaaaaaagag gctgtgacaa
    82021 aattcttgtc gcagctcctt atttcttgat gcaggacagc tttttcacaa cttcttcctt
    82081 aaacggtgtt ctcctgccaa ctcctcggcc acaagtcaca atatttataa aacatgagaa
    82141 gctgtttttt gaaattgttc cttgtggctc agagttaaac ggcaggctca agacctcttg
    82201 atacacggtg ttcaatcagc tgaccctcgg tattagatta tgtcaggacg ctaattttac
    82261 aacctcttgg taaacggtgt tcaaaagctt gcatctgcgg taataagccc aaccaaacga
    82321 aaaatataag gagctatttt ttgaaattgc tccttgtggc tcagagttaa acggcaggct
    82381 caagacctct tgatacacgg tgttcaatca gctgaccttc ggtattagct caaaaaacgg
    82441 caaaatatga ggaactattt tgccgtttgt ttctctaata ctcaggtcaa aacgctgatt
    82501 tcacaacctc tgtttttcat tgtccatagg attgatattg tccttgtgat gactcattcg
    82561 taataattga ggcttgattt tcttcacttc ccgttatcgt ttgtccactg ccagaatctg
    82621 aaggacttgt tcctgtcgac tcgttttttt gagaagagac agttgttttc ttgacttgtt
    82681 catttgtctt tttccattcc gctaaacgat cggcgaccac ttttttgtct actttttcag
    82741 aataagcggt gatcactttt ccatttttca aacgggctac tttgtcgcca tcatgagtca
    82801 aaagcaaaac ggtcaaatgc ttgtctttaa attgatattg tctgagagaa atcgcactaa
    82861 gtacgtcatc gatattaata aattgatttt cttcttttcc ttttggcaaa taaatgtcca
    82921 attcgccttt ttcgtaattg gcagttattt tatactccgc ccaccaataa gttgagaatt
    82981 cttttttgaa attatctact gattctttgc ttgctacgat tttttctgtt tctttctgag
    83041 caagatctgc tgtcattttt cctgaaaatg ctgcggataa aataagcagt aagaaaaaac
    83101 cgaataacca aaagagaaga aacggtcttt ttttcctcat gctttcctcc actcctattt
    83161 aattttgact attctattat atacaaaaaa tgaaatagaa agacaaaaaa gttgaaaaag
    83221 atcgtaaata attgaacttc tttttcacaa aatggtaaga atggagaatt tcacgcattt
    83281 gctctcaact attctttctt aagcctcaaa tccgttttta tctgatacgt ctttgaacca
    83341 atgacccgat tttttgattg ttctttttcc atcttgtgct aagtctactg aaataaagcc
    83401 atatcgattc ttatatgcat tggtccacga ccagttatcc atacatgtcc acatatgata
    83461 gcctaaacaa ttggcacctt cttgaatggc ttgatggaca tatttcagat gagattggac
    83521 aaattcgatc cgataatcat cgtggatcat gccatcttct ttgataaatc gtgcttcgtt
    83581 ttctacaccc ataccgtttt cagaaataaa gcagtcgata tttccataat gatctcgtac
    83641 attgatcaaa atatcataga ttccttgttc atagatctcc catccgcgat aaggattcat
    83701 tttacgacca ggcatttcat aattgtcgaa ataatcctca ggcattggac cgtttttagt
    83761 atcgattggt gtttcttttg cttttacccg gcgtggctgg taataattga tacctaaaag
    83821 atctactgta ttttctttga taaccgtcaa atcctctgca tggatcacag gcatgagatc
    83881 aagttctttt actatctcaa tcaattcact tggaaactcg ccttttacag ctggatcaag
    83941 gaatgaacga ttgaagaacg catctgctat ttgcgctgct ttaacatctt cagggtttga
    84001 ttcgtctctt ggatagcttg gtgtcagatt caagatgata ccaataacac caccttgctc
    84061 catttcatgg taagatttga tggctaacgc tgaagccaac gcttcatgat agccaacttg
    84121 gatcgctttt ttcatatctg actcttctgg ataatgccat cgatataaat aacctgcttc
    84181 gactggaacg attggttcat tatgagtaaa ccattttttt actttcgaac caaacaattc
    84241 aaaacaactt ttggcatatt tttcataggc ttcaaccgtt tctcggttca accatccgcc
    84301 tttttcttgc agatacatcg gcatatcgaa atgatacaga ttcacaaagg gttcgatacc
    84361 gttttcaatc aactcatcaa aataacgatg atagaaacga acggcttctt cgttgattgt
    84421 tccagattca tcaggtatca aacggctcca ttggatagat gttcgaaatg aattatgccc
    84481 agtctgtttc atcaactgta catcctcttg atacttcgta taaacttgcg aggttttttc
    84541 aggtcctact tgatcaaaga acttttgcgg ttcttttttg taccaataat caaaaatgtt
    84601 ctctcctttt ccatcgcctg aaatccttcc ttctgtttgt gggccacttg ctgcagaacc
    84661 ccaccaaaaa tttgctggaa attgatattt tttcatcaaa gcacctcttt ttctttttta
    84721 gttcgtttta gttgattcca tgtatgatat tttccatcac aatcactttt tcattttctt
    84781 cctcttcttt tttgattcgt tttaacagct gttctgctgc tcgttccccg attttatctg
    84841 ttggtggcgt gatcgtgatt ttcggaagga tactcagatc gtttttatct aaataatcat
    84901 agtgactgaa cgcaatatcc ttcccgattc tgacaccttc ttgattgaaa cattcgatgg
    84961 caccaatcga catattgtaa ttgctgacaa aaatagcagt tgcccctttt tctaataatg
    85021 cttttgcccc gtaatagccg ctttttctag aatagtctcc tgtaaagacc tctaattgga
    85081 tcgatggatc attttggacc gcttctttgg ctcctgctaa acgttctctt gctacgtagt
    85141 ctgtttgagt ggcagcaata aatccgattt ttttatgtcc ttgttcacca tgatgagcga
    85201 tcacttttgc tacactttct cgatcattca ctaatattgt atcaatacct gctggtcctt
    85261 ctggacaatt caatgcgata actggaatac tcatttttgt gagcatttca aatccagacc
    85321 actcttcttc ggataagaag acaatcagcc catcgatcac atgcttcatc aggtattcaa
    85381 ttttttgttc aattttttct ggctgatcgg caaacccgct taataataag ctatacccat
    85441 tttcttcagc tactctttcg atccctgaga caacttccga cccaaaacgg ctggaaatcc
    85501 cgtttatcaa aagtccaata gagaagcttc gattattctt caacccttta gcaatgatgt
    85561 tttctttata gtccaacgct gaaatcgctt ctgctatttt tttcatgttc ttttccttca
    85621 gttgctgacc attcaagtaa cgggaaactg ttcccactga aacaccagcc atcttagcca
    85681 catcacggat cgatgccata cattctcact cctgccagtt acatttgatt tctctttctt
    85741 gttgttcgat tatgatgacc gcattctttg catagtgttg cttgtcatca atatccatat
    85801 aactaaagtc ttcagtagag caacttcctc tcactattaa ttatacttaa acaagtattt
    85861 gaaccggttc atttttaata tagcattttt ttaaaagatt gcaacacttt tttaaaacgc
    85921 tttctgaaag tggcggattt tttgcctttt taaaatttta ttctaaaatt tactctattt
    85981 gtgggtgttt tgtgcgtttt tctcttccat ccgtttgaag ttttacattc caatggtata
    86041 atttctttat cctatcaaat gggggaagta ttttgaagaa attaatcgta ctatttattt
    86101 tgtctgcggg acttttgaca ttgactggct gtgatttgtt tggcggctct gaccaatcct
    86161 cggacgaaac aaccgattct tctgtcttaa aagcggaaaa agcagatacc accaacagtg
    86221 tatatacgct aaaagtacgt aaagatatgg atttatccgc gggacagttg acatttacag
    86281 ataaaaaaat ggaatggaca cgcacgtatc agacacaagg aactgattct tctgaatcat
    86341 ctgaagaaaa aacagtgctg acagatatca agatcactac caaagatgac gaatatatta
    86401 tttcaggaaa agaaaataaa aaagaaacaa cagtcatatt tacaaaaatc ggtaattacc
    86461 gtttgaagga tgaggatggc aacatctaca gtctatagtg ccgtctgttg gatcttcttt
    86521 tgtttctccc cttcttttct gctatagtaa agaagcaaga tgattgtaaa ggagacgaaa
    86581 tggatgatcg atagtcctga ttattgtaca tgtaataaca acaaaaatat ccatacagaa
    86641 tttaatgacc aaggaagctt gagcatatgc gacaactgtc acaaactagt caaagattca
    86701 tttaaaacat ttgaatctta tcctgttcaa aattatgtaa aaagtagaag aagataaaca
    86761 aatccaagca aaactcacta tggctgattt ttatgaactt caaactcagc ttcttccttt
    86821 gatcaagctt catcagtaaa acaaacagtg gatcatttca gtttacaaat caaagagtaa
    86881 agatttattt atttcaaata tttgaatata aaataaccaa acgatagaaa ggcttattct
    86941 acatcacgta gtaatttttc tttcccgtcg tttggttttt
//
LOCUS       DANKEF010000005        83001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000005 DANKEF010000000
VERSION     DANKEF010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 83001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 83001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..83001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.005"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..563)
                     /locus_tag="PWZ48_000376"
     CDS             complement(<1..563)
                     /locus_tag="PWZ48_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HDL2260057.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="PWZ48_000377"
     CDS             complement(750..1400)
                     /locus_tag="PWZ48_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260058.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="PWZ48_000378"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="PWZ48_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HDL2260059.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="PWZ48_000379"
     CDS             complement(2912..3910)
                     /locus_tag="PWZ48_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HDL2260060.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="PWZ48_000380"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="PWZ48_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDL2260061.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="PWZ48_000381"
     CDS             complement(4926..5657)
                     /locus_tag="PWZ48_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HDL2260062.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="PWZ48_000382"
     CDS             complement(5661..6926)
                     /locus_tag="PWZ48_000382"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HDL2260063.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="PWZ48_000383"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="PWZ48_000383"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDL2260064.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="PWZ48_000384"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="PWZ48_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HDL2260065.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="PWZ48_000385"
     CDS             12268..13770
                     /locus_tag="PWZ48_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HDL2260066.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="PWZ48_000386"
     CDS             13772..14509
                     /locus_tag="PWZ48_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2260067.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="PWZ48_000387"
     CDS             complement(14554..14727)
                     /locus_tag="PWZ48_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260068.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="PWZ48_000388"
     CDS             14703..15368
                     /locus_tag="PWZ48_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260069.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFFEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="PWZ48_000389"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="PWZ48_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDL2260070.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="PWZ48_000390"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="PWZ48_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDL2260071.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="PWZ48_000391"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="PWZ48_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDL2260072.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="PWZ48_000392"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="PWZ48_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HDL2260073.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="PWZ48_000393"
     CDS             complement(20581..21747)
                     /locus_tag="PWZ48_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDL2260074.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="PWZ48_000394"
     CDS             complement(21763..21990)
                     /locus_tag="PWZ48_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDL2260075.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="PWZ48_000395"
     CDS             22474..23064
                     /locus_tag="PWZ48_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HDL2260076.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="PWZ48_000396"
     CDS             23254..23967
                     /locus_tag="PWZ48_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260077.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="PWZ48_000397"
     CDS             complement(24082..25092)
                     /locus_tag="PWZ48_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HDL2260078.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="PWZ48_000398"
     CDS             complement(25129..25611)
                     /locus_tag="PWZ48_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HDL2260079.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="PWZ48_000399"
     CDS             25761..26129
                     /locus_tag="PWZ48_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HDL2260080.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="PWZ48_000400"
     CDS             26594..27046
                     /locus_tag="PWZ48_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260081.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="PWZ48_000401"
     CDS             complement(27170..28021)
                     /locus_tag="PWZ48_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HDL2260082.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="PWZ48_000402"
     CDS             complement(28034..28819)
                     /locus_tag="PWZ48_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2260083.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="PWZ48_000403"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="PWZ48_000403"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HDL2260084.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="PWZ48_000404"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="PWZ48_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDL2260085.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="PWZ48_000405"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="PWZ48_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="PWZ48_000406"
     CDS             34217..34390
                     /locus_tag="PWZ48_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2260086.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="PWZ48_000407"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="PWZ48_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDL2260087.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="PWZ48_000408"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="PWZ48_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDL2260088.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="PWZ48_000409"
     CDS             complement(37240..37551)
                     /locus_tag="PWZ48_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HDL2260089.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="PWZ48_000410"
     CDS             complement(37548..37841)
                     /locus_tag="PWZ48_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HDL2260090.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="PWZ48_000411"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="PWZ48_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDL2260091.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="PWZ48_000412"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="PWZ48_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDL2260092.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="PWZ48_000413"
     CDS             complement(39679..44037)
                     /locus_tag="PWZ48_000413"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HDL2260093.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="PWZ48_000414"
     CDS             complement(44249..45958)
                     /locus_tag="PWZ48_000414"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDL2260094.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="PWZ48_000415"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="PWZ48_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HDL2260095.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="PWZ48_000416"
     CDS             complement(47455..48255)
                     /locus_tag="PWZ48_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDL2260096.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="PWZ48_000417"
     CDS             complement(48252..49064)
                     /locus_tag="PWZ48_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HDL2260097.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="PWZ48_000418"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="PWZ48_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDL2260098.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="PWZ48_000419"
     CDS             complement(49819..50541)
                     /locus_tag="PWZ48_000419"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDL2260099.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="PWZ48_000420"
     CDS             complement(50677..51558)
                     /locus_tag="PWZ48_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDL2260100.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="PWZ48_000421"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="PWZ48_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDL2260101.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="PWZ48_000422"
     CDS             52798..53277
                     /locus_tag="PWZ48_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HDL2260102.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="PWZ48_000423"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="PWZ48_000423"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HDL2260103.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="PWZ48_000424"
     CDS             54578..54706
                     /locus_tag="PWZ48_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2260104.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="PWZ48_000425"
     CDS             54710..55255
                     /locus_tag="PWZ48_000425"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HDL2260105.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="PWZ48_000426"
     CDS             complement(55709..57400)
                     /locus_tag="PWZ48_000426"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDL2260106.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="PWZ48_000427"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="PWZ48_000427"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDL2260107.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="PWZ48_000428"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="PWZ48_000428"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDL2260108.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="PWZ48_000429"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="PWZ48_000429"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HDL2260109.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            61682..62383
                     /locus_tag="PWZ48_000430"
     CDS             61682..62383
                     /locus_tag="PWZ48_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2260110.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(62992..64008)
                     /gene="tsaD"
                     /locus_tag="PWZ48_000431"
     CDS             complement(62992..64008)
                     /gene="tsaD"
                     /locus_tag="PWZ48_000431"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDL2260111.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(64005..64469)
                     /gene="rimI"
                     /locus_tag="PWZ48_000432"
     CDS             complement(64005..64469)
                     /gene="rimI"
                     /locus_tag="PWZ48_000432"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2260112.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(64476..65018)
                     /gene="rimI"
                     /locus_tag="PWZ48_000433"
     CDS             complement(64476..65018)
                     /gene="rimI"
                     /locus_tag="PWZ48_000433"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDL2260113.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(65002..65724)
                     /gene="tsaB"
                     /locus_tag="PWZ48_000434"
     CDS             complement(65002..65724)
                     /gene="tsaB"
                     /locus_tag="PWZ48_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDL2260114.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(65915..66895)
                     /locus_tag="PWZ48_000435"
     CDS             complement(65915..66895)
                     /locus_tag="PWZ48_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260115.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(66919..68403)
                     /gene="galT"
                     /locus_tag="PWZ48_000436"
     CDS             complement(66919..68403)
                     /gene="galT"
                     /locus_tag="PWZ48_000436"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HDL2260116.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(68415..69404)
                     /gene="galE"
                     /locus_tag="PWZ48_000437"
     CDS             complement(68415..69404)
                     /gene="galE"
                     /locus_tag="PWZ48_000437"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDL2260117.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            69652..69819
                     /locus_tag="PWZ48_000438"
     CDS             69652..69819
                     /locus_tag="PWZ48_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HDL2260118.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(69881..71692)
                     /locus_tag="PWZ48_000439"
     CDS             complement(69881..71692)
                     /locus_tag="PWZ48_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HDL2260119.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(71689..72054)
                     /locus_tag="PWZ48_000440"
     CDS             complement(71689..72054)
                     /locus_tag="PWZ48_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HDL2260120.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(72217..72612)
                     /locus_tag="PWZ48_000441"
     CDS             complement(72217..72612)
                     /locus_tag="PWZ48_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2260121.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(72630..73592)
                     /locus_tag="PWZ48_000442"
     CDS             complement(72630..73592)
                     /locus_tag="PWZ48_000442"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HDL2260122.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(73592..73804)
                     /locus_tag="PWZ48_000443"
     CDS             complement(73592..73804)
                     /locus_tag="PWZ48_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HDL2260123.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(73825..74523)
                     /locus_tag="PWZ48_000444"
     CDS             complement(73825..74523)
                     /locus_tag="PWZ48_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2260124.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(74543..75085)
                     /locus_tag="PWZ48_000445"
     CDS             complement(74543..75085)
                     /locus_tag="PWZ48_000445"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDL2260125.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(75217..76221)
                     /locus_tag="PWZ48_000446"
     CDS             complement(75217..76221)
                     /locus_tag="PWZ48_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2260126.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(76218..77207)
                     /locus_tag="PWZ48_000447"
     CDS             complement(76218..77207)
                     /locus_tag="PWZ48_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HDL2260127.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(77204..78010)
                     /locus_tag="PWZ48_000448"
     CDS             complement(77204..78010)
                     /locus_tag="PWZ48_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260128.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            78176..79132
                     /locus_tag="PWZ48_000449"
     CDS             78176..79132
                     /locus_tag="PWZ48_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2260129.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(79209..79727)
                     /locus_tag="PWZ48_000450"
     CDS             complement(79209..79727)
                     /locus_tag="PWZ48_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2260130.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(79815..79964)
                     /gene="rpmG"
                     /locus_tag="PWZ48_000451"
     CDS             complement(79815..79964)
                     /gene="rpmG"
                     /locus_tag="PWZ48_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2260131.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            80192..80638
                     /locus_tag="PWZ48_000452"
     CDS             80192..80638
                     /locus_tag="PWZ48_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDL2260132.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(80833..82728)
                     /gene="asnB"
                     /locus_tag="PWZ48_000453"
     CDS             complement(80833..82728)
                     /gene="asnB"
                     /locus_tag="PWZ48_000453"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HDL2260133.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        23580 a        17673 c        14336 g        27412 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacacttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt ttttgaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg gcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacttagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttattcatt
    61381 ttttattctg tatacacttt caaaaaaata tttattcatt gaaaaaattt ttttgaataa
    61441 attaaaaaaa taaattatat tatcgttgtt agcgtttact atatgtatta ttgttgtttt
    61501 ttatacacaa atcattcgct tcaaaacaaa ttattcaata ccattgtata ttttttacat
    61561 aataacaaat tatatttgta aagtattaaa acttgtttct aatgttggta tgttaaaact
    61621 ttacaaattc tttaaaaaaa gtgacaataa atatagtgaa caataaggag aggacttttt
    61681 aatgcgtgtt aataaaatcg attttcactt ttcaaaactc agatatcaac ctgattttga
    61741 acatttctcg gatgaagagt tccaacagat caaggaacat actgttcttc gttcttataa
    61801 aaaaggacag atccttttcg acgatggtga tcccagacaa cggttgtata tcttgaccaa
    61861 aggactcgtt cgtttagaac gttatgatga aagtgcaact tattactact atgactatgt
    61921 gaatacaaat aacctttttc ctatgtcagg aatgtttgga gaagagacct acacttatac
    61981 agctcaagcg atgactgata ttgaaacatt ctaccttcct gtttcctact atgaaaaatt
    62041 agcacgaaaa aatcccgaac agttgatcta tatgatacaa aaaatttcta aagtagttga
    62101 aatacatgaa ttgcgtatcc aaatcggatt gacctctagc gcctttgacc gggtaaaaca
    62161 aaatttattc attttgaaag aagaattggg cgtaccaaca aaagatggtc atatcaccat
    62221 tccttatccg atcaccttaa aagaattagc agtgaatagc ggcacgacac gtgaaactgc
    62281 tggacaagta atcaaacaac tacgtgattc cgggctgtta gattatgaca aaaagcgttt
    62341 tactttctgt aagacagcta ctactccagc tatgagagaa taacgcgaac aaaaataagc
    62401 agtgaccctg ataaatatca gggtcactgc ttattttcct ccctaaaact tctgaaagat
    62461 ccggcagttt tagatcactg gacaagactc aaagattttc tatgtgtgtt tcagttatgt
    62521 aatattcact gtttgcaaaa aatactgtta gattaactag atgaagcgtc ttgtcctcat
    62581 ccgatctaga ttattcgata ttcctcgaca ataaacgatt actcagtgag ggcaatcaga
    62641 ggaacggata atgataaata agctgtaaaa aatagcaaaa taacaataat gattaaaagt
    62701 atgtaaaaaa tagggctttg acaaaaacgt gtcacagccc tatttcgatt aaacggtgtg
    62761 tacctgtcaa ctcttcggtc acaagtcata atgttcataa acatgaggag ctgttttttg
    62821 aaattgttcc ttgtgaatca gagttaaacg acaggcttac gaactcttga ttaaacggcg
    62881 ttcaaaagct tgcatacgcg gtaataagaa cagccaggtc aaaaacatga gaagccgttt
    62941 ttcctgggct gttcttatta cttgatgcag ggcagctttt tcacaacctc tttattcaga
    63001 taaaaccaag cttggatcag cattcaactg atagctagca aaatttcttt tttgcatttc
    63061 cacatgtgct gctgctccaa tcattgcagc attatctcca caaagtcgaa gagggggaat
    63121 cactagttct acctcaggga gtttagctgc caaagcatcc tgcaatcctt cacgcaaccc
    63181 ttgattggcc gctacacctc cagctataat cagttgcttg actggataat tttgacaagc
    63241 tcgaagtgtt ttagtaatca atacatcgat cacacttgct tgaaagctag ctgctaaatc
    63301 atttttgtct agatcttctc cacgctgttg tgcattatgc accaaattga taaatgcact
    63361 ttttaatccg ctaaaactga aatcataatt atcttcatgg atcatcgctc gcggaaaatg
    63421 gtaattatct tttccctgat gagctagctg atcgatttct tttccgcttg ggtaagaaag
    63481 acctaatact cttccaactt tgtcataagc ttctcctgct gcgtcatctc ttgtctcccc
    63541 aatgatttca tatgatccat cttcttgcat atagactaat tctgtatgtc ctccgctaac
    63601 aagaagtgcc atcaatggaa actggaatgg tttaaccaga cgggctgcat agatatgccc
    63661 tgccatatgg tttacaggaa taagcggcaa ttggtgtgcc caagcaaaag ccttagctgc
    63721 agaaatacca atcagcaacg agccgacaag tcctggtcca taagtgacag cgactgccga
    63781 taaatcttct gccgacacac cagcttctac cagtgcatct tccaagcata aggtgatttg
    63841 ttctacgtga tggcggctag ctacttcagg tactacgcca ccaaaacgtt tatgactgtt
    63901 tatttgagaa gcaacgatgt tagataagat ctctgttcca tttctaacga ccgctacact
    63961 cgtttcatca caacttgatt caatcgccag aatcaattct tcattcattt catcgtctcc
    64021 gtttttaact ttctgctcat aatgatcgca tcttccgtcg gattgcgata atatgatttc
    64081 cgtttaccta atatacgaaa cttttctgat tcataaagtt ttcttgctgc ttcattagat
    64141 agacgtactt ctaaaaaaac atgcagcgaa tcttttattt gttccgtaat cagtaattgt
    64201 aacaaatgcc gtgcatgccc ttttccttgt tctgaaactg caacagcaat atttgtgatc
    64261 tcgacttcat ctattacttt cgagtatcca ataaagccac agatctggtt atcttcagct
    64321 aaaagcagat aatcagagtg aggctgttgg atgtctgcca aaaactgcat tttcgtccaa
    64381 ggtgaaccat gtggataagc attttcgcta atcatccata cgcattctgc caagtacgct
    64441 tcatcaaaat ctttttttct gcaaatcaac taatctcact cattttctaa aagttttatc
    64501 atatccaacg catcttcttt tgtttcagtg taatatcttt ttttgatcgt tgttgcttga
    64561 aaccccaatt tccgataaag tctttgtgca tcttgattac tcatacgcac ttctaatgac
    64621 atcgtctcac agcgattcat tatagcaaag ttctcgattt cgtcaataag cagcgaaccg
    64681 attcgtttac gctgatatgc cggtcgaacg gcaatattcg tgatatggca atctaaaccg
    64741 aacgctcttg aaccgataaa accaatcatt cttccattat caaagatacc tagatataga
    64801 tgaggaagcg gtgacttcaa ttctgatata aaggcacttt ttgtccatgg caattcgcca
    64861 ttatagactt ctcgttcgac atccagcagc tctttgatat cttctgtagt caattctctt
    64921 agtacgtaac gacgatcgat ttgttcgact gttttttctg tataaatcat tttatcttga
    64981 aaaaagcttc tggcatacgc tctaaatttt ttcaacataa ctttcgtctc caggtgtgtg
    65041 cgtctccaac catttttctt ctgcttctac tcgttttaaa tagcgaggaa gaaaagcttg
    65101 tttctctgca actggttcaa ctaaagcacc taattgagcc acagttacac cacttggaat
    65161 atcccatgct ggaactgtat taatcatcgc actaggcaat atttccttta tttcagagaa
    65221 gaatttgtga caatcactcc cgacgaaata aatagcttca ttcagctttt gtagttgtgt
    65281 gaacaaatca gaagcagaaa tatgtacatc ttcaagtaca gtttctaatc tattttcttt
    65341 ccagcggtat gccccagcgt atacattttt tcttcgagca tcaaacatgg gcacaataat
    65401 attatccaca ccgatgcaat tagccgcgat ggttttcaga ctagatactc ctactagctc
    65461 tttatctaat gtataagcaa tcgtttttgc tgtcgttaca cctatacgaa gccctgtata
    65521 agaaccggga ccatcagaaa ccgcgattcg gtcaatgtct tttggactta tttgaagatc
    65581 tgcaaacaat tggtcgattg caggcatcag tgttacactg tggttgcgtt tgatatttgt
    65641 ttgtatttgt ccaagaattt gttcgtcttc taccacaccg atcgccaaag tttggtttgc
    65701 agtatctatt cctaatgtaa tcatatattt gatcctttct gctatgacat tctcgccttc
    65761 cattgtagca tactttttta ttctgaggat gaaaatacca aaggcaaaac tagtacaata
    65821 atacatgatt atctgctggc gggcaaattt tgccccttcc tgaaacacaa aaaagctgcg
    65881 gtatcactcc attttcccac agcttttcca tcttttattt cgttgattct cgaataatca
    65941 actctgtttc cacaataatc tttcttgaaa ccggtgcttt ggaattactt aattgaagaa
    66001 tcgtctccac tgctagctct cccatccatt cggtataaac ttttacagta gaaagcgagg
    66061 gcgttacata tttcgctaca ctgacatcat tgaagcccat cacagaaata tcttctggta
    66121 ccactaaacc tttttcttga atcgctttca gtgcgccaat cgctaatgca tcattagctg
    66181 caaaaagcgc atcaggaaca tgcggattcg tttttaaata ttgcttcact gcttgatatc
    66241 ctgactcgac agaaaaatct gcttcgataa ttggaccttc gtcgtagcct ttttctgcca
    66301 aaacttgttg aaatgccata attctgggat cttttagccg ctgatggttt ttcttcgtat
    66361 attctgttcc agcaagcata gcgatatgtt gcgctttatg ggagacaaag tgttcaatga
    66421 ctttttgaac acttgactta aagtcgacaa ctagagagtc gattccttga gaagtgccgt
    66481 cagaatctac caaaagcaag gcattttggg atcctttcat ttctaataac tgagcttcgt
    66541 cgaattttcc tagtgcgatc gttccatctg ccggaagatc actaatggaa tcccacgttt
    66601 ctttcaataa agtaacgttt gcttcttctg cctttttttc gattcctagg cgaatagata
    66661 aataataaag atcttcaagc tcttccgtat cgtcgtacca ctggatcagg cgaagcgttt
    66721 gcttagtatt tgctgcattt ttacgatgtt tcgtataatt taagcgttct gctgcttcaa
    66781 aaattttttg ctttgtggct aagccaacgg aaagatcagg atcatagttg agaactcttg
    66841 aaacagtagc aggagaaact cctgccaact gcgcaatgtc tttaattgtt gccatttgtc
    66901 tctcctcctt ttttacgttt ataaaatcgc agtaaatcgt tcaaacgctg cctgtccttt
    66961 ttcatctcgc ttgaatactc cagcgtcttc cagtacacgt gcaaaaacct gacctacaga
    67021 tttttgtacg acatcggtga ccgtatcttt cgtcaaatca ggatgttctt tttttaattg
    67081 ttctgcccac ggacgatgat attcagccat ctggttttct tctcctaata agaaatgctc
    67141 tacttctact agttctggtt tcaaacgagg tggtaaaaca gcaagcccca ttacttcgat
    67201 caacccaatg ttctcctttt tgatatgttg gacatcttta tgtggatgaa aaatcccatc
    67261 aggatgttct tctgaaacat tgttgtctcg aaggacaagg tccaactcaa agtaattgtc
    67321 ccttcttcgt gcaattggcg taatcgtatg gtgtggtgta ccatctgctg aaaaagctct
    67381 gatagatact gtttcatccg aatatgcttt ccatttttcc aatactgagt tagctgcttc
    67441 aataagcaag tttttctctg ttgattgcaa acggatcaca gacatcggcc atttcacgac
    67501 tcctgcgatg acttgcggat aatcttctag tttgataagt ttttctatcg gtgcttttgc
    67561 cataggaaat tcatgacgtc ctgcctgata atgatcatga gaaagaatcg atcctccaac
    67621 aattggtaag tcagcattcg aaccaacaaa gtaatgaggc aagacttcag taatccgaag
    67681 cagtctagca aatgtttcct ttgtgatttt catcgggcgg tgttgttctg ataaaatgat
    67741 cgaatgttca ttgtaataag cataagggga atattggaat ccccagcttt caccgtctag
    67801 attcattcga ataatccggt gattggtgcg ggctggatag ttcaagcgac ctctatatcc
    67861 ttcgttttcc atgcaaagca tgcaggtagg atagtctact tttaccgcct cgcgttctgc
    67921 tgcgatttgt tttggatctt tttctggctt agataaattg attgtgattt ccagttcacc
    67981 atactccgac tctgccggaa atttgatgtt ttttgcgatg gctcttgttt tgatgtagtc
    68041 atttttctta cttaaattaa agaaatactc agttgcttcc tctgggcttt tcgaatagtg
    68101 ctgtgcaaag aatgcattga cgactgaagg tggcggtgtg agaaaatcca ttaattgcgc
    68161 ttcaaaaatc tcttgttctg ctggcaattc tgaaagaaca ccattttctc ttgccttagc
    68221 gacaagctga tccaataagt ctacagattc ttccttaact ggtcttactt caatgctttc
    68281 tagagcatct tctcctacca tccctaaaag acgattctgt aaatataagc ggtccatttc
    68341 catccatccg ccagcttcga tcgcaagtgt ggcaaaatca caaatcgttt gactgattga
    68401 catatctcat tcccctattc ttcataacca tttggatggc tgacatgcca atcccatgct
    68461 gttttgataa tcgctttgat atctgtgtat tcaggtttcc agcccagaat ctctctcgct
    68521 ttttcacttg aagcgactaa tcggctagga tctccagctc ttcttggtgc tatttctgct
    68581 ggaatttctt ttcctgtcac ttctctagct gcttctagca tttccttgac tgagtatcct
    68641 ttgctgctgc ctaaattgaa gaagttgctt tcatttcctt ctttcaagta ttccaaagct
    68701 aaaatatgtg cagcaatcaa atcttctact tgaacataat ctctgataca tgttccatct
    68761 ggtgtatcat aatcgtctcc ataaacagcc aaggcttttc tttgaccaag agctacttgt
    68821 aaaatgattg ggacaagatg cgtttctggt gtatgatctt ctccgataga tgcatctgct
    68881 tttgctccgg ctacattgaa gtagcgcaaa gcgacatagc gcatgccata tgcttggtca
    68941 caccacttca tcattttttc catcatcaat ttactttcac catacggatt tttaggatta
    69001 gctggtgtat cttctgtgat tggagactct ttcggctctc cataagttgc tgcagtagaa
    69061 gagaatacga ttttattcac gttgaactcg tgcatgactt caagtaagat ttgcataccg
    69121 tatacgttgt tgttgaaata catcaatggt ttttcgacag attctcctac taaagagctt
    69181 gcagcaaaat ggattactcc ttcaatcggt tccttttcaa atacggaacg tagaaattct
    69241 ttatcccgga tgtctccttc ataaaaatga gcatctggat gtactgcttg tttgtgacct
    69301 gtgagcaaat tatctaccac agctacttga tacccctttt gaactagctg gtctacagcg
    69361 tgcgaaccga tataaccggc tccgcctagt actaaaattg tcataaaaaa cactcctttg
    69421 attagtaaaa ttgtgtttgt tgattctata taatttaaaa aacgaagttt acttttatgg
    69481 tgaaaacaaa caaagtaaaa tttcgtaaat tcatatggta aatttataat aaaacattta
    69541 gtaaacattt tcaagggttt tttgcaaagg tttacaaaca aacgtaaaac tagccttttt
    69601 ttctcttttg ttccataata gatataacaa ataataaatg aggtggattc aatgaaaaaa
    69661 tttgtatctg gaatattagt aggaagtctg gcaacagccg ctgccgttgc tggtttggtt
    69721 gcttctgtga aaaaaacagt gatcgatcca attgacgaaa aagaagcaat gatcgaagaa
    69781 aaccgtaaaa aagcaatgcg taaacgcgtt tctcgttaat aattatttaa aaatacgcat
    69841 gaagactttc taaaaaacag ataagtcatc atgcgtattt ttatttgtca gtcaatataa
    69901 gttgtgctat tttatctgcc tgtctcagat tccatggatc tgtataaggt ctagctactg
    69961 ctgcagttag ccaatccaca ccttcaacag gtattccata gcaataaaag tcagactgga
    70021 tttcccctga agcatcaatc acgcgattcg tacctcgctc aacatctata gcttctgaat
    70081 gataacttcc tgtattatct gaaaatgaaa aagggcgaac aagtttgttt tccttcaaat
    70141 tttttatgac aggattactt gaacgagaaa agcttgtttg tggcaatcga gcttcaatca
    70201 aatagcgact gctgatttct ttatcttgta gagaaataac atagcggttg tgcttttgct
    70261 gaatatcaaa tggcggttcg atcaaatgaa aaatacccgc atctatcagt gctgctaatt
    70321 ctttcgtccg aatgattggc ggaccgatcg ttaaaaagct atttagatga gtaaaccatc
    70381 cccatagtat ttctttgtat tccttagcag aaaaaacgtg ccattcaata gcctgccgga
    70441 ttggatcacg ccagtctttt aatgcatcaa atgttgaagc aaatgctcct gtgcaattgc
    70501 ctttttccgc ttcttttatc tgcccttcaa taaatattcg gctagcttct gaaaagggct
    70561 cattctgata ggcaagagga gtttctaaat acgaccaatc ccatctataa gggattagtg
    70621 ctggatgctt tccgagacac tcctctgaag agagggagag aaaatcatgc tcaaaatttt
    70681 tctttggaat cggcaagtga tattcttcga tgattttctt gtaataaaac aactcaacgt
    70741 cttttttcag atagtcaaaa aatgtttcgc cagtcaaaaa tcccgactga tgccattgct
    70801 ccaggttttc ttttgttagt aaccgtggcc atgccattgc tccgccttgc ttttgatttc
    70861 ttcctcgagg gtaataaggg agcccccttc ttgatccact gtatacaatc ggttccttgc
    70921 ctgatggatg atagaccagt tttccctttt cgttctcgaa gcgccctcct cttgatactg
    70981 taaacaatcc gacataatca aaaaaactta agcctagacc tcttaaaaag acattttctt
    71041 taggcggtat cttcgaaaga tctacatctg caggattaga aggaagctga tacagtaaaa
    71101 catttttctt tgcgtagtct acccacactc tttcttcttc tgtctcatca ttttcccagt
    71161 gaccgcttgc taaaacaagc tgatcgatag aatagctttg ctgatctgtc tgtaaaagat
    71221 aaccatcttt tcttttgctt acttttttta ctaaagaata aacaaactga atagaagctg
    71281 gaaattcttc ttgcaacttt gagaaaaacc agcgttgata gagtccgtat aaacatcgag
    71341 tagattgctg atctttttcc agacgattga tttcttctat aattagttgc ttatttttgg
    71401 ttaccgtctg ttcgatataa tctgtagcag gtcctttact ccattgaaac aagttgggtc
    71461 cctgtgttac caatcctcca ctggacaatg tttcatctgt aaaaagcgtc acttgttgag
    71521 aaacagaatt cattaataac tccggtggct gattcaaccg ccaaactcga cctccaggac
    71581 cattgggatc gaaaagaaaa agttcaagag aatgtctgtc ctgattatta tggagaatcc
    71641 tttctgctac acttaatcct ctaggtccgg caccaatgat ccctattttc atttgcatgc
    71701 ctccatttcg ataaattttt tcaataaaac agcatggttc tgatgttcat tttttgcacc
    71761 ataaacaaaa gtaatgactg gatgttcttt cataagttga ctgagtttaa aaatagcttg
    71821 cttcttttct tcttcgaaaa tcaattcttg ctggtatttc tttgcaaatg aagcaaatcg
    71881 ttctggtaca tggtcaaaag ttttgcgtgc ttcattacta ggtgcaattt ctttcaacca
    71941 cagatctaac tgcgcttttt cttttgaaat tcctcgtggc cagaatctat ccacgagtat
    72001 tctatagcca tcctcttttt cagcatcaag gtaaacacgt tttaatttga tcatagttgt
    72061 ttccttcttt ctaccttttc gcttagctta tatatgtaga taatagcaca aaataaaaaa
    72121 aatgaacggc acaactatcc attgcgccgt tcatgtgatc ttggtcaaaa tctaatgatt
    72181 gattcggttt ttaaagctgt catacaatca tctgttttat acttttttac ttttgatttt
    72241 tccagaccat ccgtcatatc cacccttaag taaataaatc tcttgataac ctgctttacg
    72301 taatttattt gctgtacgga tgctcaaact tttcttttga tcgtaaatat agacaggctg
    72361 gtcttttcga atagatccta atgaatcttt caaaacagtg taagggatgt ttcttgcacc
    72421 taaaatatgc ccagcatcaa acacatcttt ttcgcgaaca tcgattactt gtgcttttct
    72481 cattccttct cgaaattctt cttctgtcaa agtcgttgct gaacgtttgg ccattactcg
    72541 tagatagact tcccaaagga tgattgcaag cacaatcaat agcaaaatga tattaattac
    72601 ccataaaacc atgtaatgcc tccgtttttt taagcaaatt tcaaggctaa tgaggcagca
    72661 ccaatgacac cagcttcatt accaagctct gctaatttga tttttgttga ttctcttact
    72721 tgcgggaaag taaattcttt aaaatatttc tctacacggc tacgtaaaaa ttctcctgct
    72781 gccgatactc ctccacctaa cacgatactt gacggattta atgtattccc caagtttccg
    72841 caagctaaac ctaagtagaa gcatacgcga tcaacaacca tcaatgcaaa cggatcgtct
    72901 tcttgagcca tttcaaagat atctttactt gttacttctt ctccgttgtc tagcattgct
    72961 ttcagttttg aatcccctgc gtattcttct gccaagaaac gacctaggcg taccacacct
    73021 gtagcgctag atacagtttc taaacatccg cgtttaccgc aagtacattc gaacccttca
    73081 ggatccacag tgatatgtcc gatttctcct ccagctccgc tcacgccgtg aatcaaattt
    73141 ccgccagcaa cgatacctcc gccaacacct gttcctagag taacgaatac aacgtctgga
    73201 tcgttttctc cagcaccttt ccatcgttca cctaaagcag caacattcgc gtcattatcg
    73261 atagcgaatt tgattcctgt tcctgcctcg atcagttttt tcacaaattg aacttctgtc
    73321 cagtttaagt tatacgcacc aataaccgtt ccagcttcgc tgtccacgct tcctggggta
    73381 cccatgccga ttccgataaa gtcttcggct ttcatattat ataaattcaa acgatgattg
    73441 attgattcaa tgatttcagg aacgatatgc ttaccatcat ccaaaatatt ggtatcgatg
    73501 ctccattttt gctgaatctc tccttctggt gttaaaattg caaattttgc tgttgttcca
    73561 cctaaatcaa tcccaataat cttcttgtcc attacaaatt tcctttcttt cggctttctt
    73621 caattcggtg ctctcttttt aatatgcgcc acgctgtcat gtatgtatca tgatcaatca
    73681 aacggctatc atacagatta cgcaattcga tagtcattaa ttcaatgtca tagatgcgtg
    73741 cacccacata gataaagatg ccaaattttt tgaacagctg ctgtacatca tataagctct
    73801 ccatctttaa gcacttcctt catattatac aagtaaacca tattttttta aacccagtaa
    73861 aatacatatt acaaaaagaa atacaaaaac tagtgtagaa agaatccttt ggtgtatcga
    73921 atagcttgtc tgttgtttcg gtagacccaa aacattccct aacactaatc cgccaattag
    73981 cccaccgaca tgtccccaaa catctacaga ggtatccaga agaccaaaaa caaatgtcaa
    74041 cacaataaat aaaagaaaat gtctgactag ctgctggatc gctgggttat gtttaaaatg
    74101 aaaccctaag ataaacaacg caccgaacaa accgaatatc gaagtacttg caccaccaga
    74161 cagaacattc gcttcgttaa aagcaaagct tgctgtattt cccattatcc cgctgaacat
    74221 ataaatcaag aaaaaacgcc aatggccata gattgcctcg atttgctgcc ccataaaata
    74281 cagtatcagt gagttcatta cgaaatgcat cagtccaaaa tggatgaaga ttggtgtcac
    74341 taaacgccac cattcattga agtgaacaag ataaggacca tacattccgg cataataagg
    74401 gatctcaagt cgcggaaata attccatcat gaggaagacc gctgtctgta tccctaaaaa
    74461 agcataggtc cataacggtc gtcttaacca tgcacgcaat tttatttgct gttgataatt
    74521 catgaaaacc tcccatttca ttttagtcaa taaacaattg ttggatcttc tgatcaaagc
    74581 tctccggttg ccattgttca ttcaattgtt cacgaaaaac aaggctacat gtttttttat
    74641 gaaaattatt taaataccgg tcgtaataac caccgccaaa accgattcga taaccggctc
    74701 tattgtagat aatacctggt acaatgagga gatcgatcat ctccggcaaa tagagcttct
    74761 ctgattgtgg ctcaagaaca ccaaaactcg aacgtacaag aacggactgt tcatccattt
    74821 cataaaacgc catctgacgt ttcggcaaag ttttaggtac aactattttc ttttgttctt
    74881 tttctgctcg ttggataacc ggctgtgtat ccagttcaaa ttcattcgat aaagtcgtcc
    74941 ctatcacttt tgctgacttc cagtactcgc tggcgaatag ctgtttatag attcgctgtt
    75001 ctttttcctt tttcttgcca cttttggcca gatttttcag cttttcgatt cctttccggc
    75061 gtaatcttac tttcaattgg tccaaaaact cacccttcct tctctcataa aatggtatag
    75121 gaaaaaaaga aaaatagcaa tcgaactttc aaataaatgc ataaatagaa taaaaagaag
    75181 acctagaaat gattggcctt ccttttttaa tgaatttcaa ctgttcagcg cgtgtttttg
    75241 tctgataaaa agtgcataca gaaaatatgg cgcaccaaaa agggcaatca aaattcctgt
    75301 aggtacttcc ccacttggta gaatcactct agcaaccata tctgcactaa gcagaaaaat
    75361 ggaaccgaac aaaccgctca gaacaaaggt ccagttattt tctttacgta cgactagctt
    75421 cgcagcatga ggagctagta gtcctacaaa agataaacta cctgtcaccg ctacactacc
    75481 gcttgccaaa gcaacagcta acaacaaaag gattttttga cttttagcca cgttaaggcc
    75541 taatccttgc gcgcgttctt gaccaaatga tagcaataca atcctctgtg cataaagtcc
    75601 tcctagcgga attaagataa ccgcccatgg aagcaatgcc caaacataag gccagctcgc
    75661 accccagatt gatcccgcta accatgcatt cacaaatcca taattttcct tagataattg
    75721 gatcgtgcat agcaatgtta atgcagaaag gccggcatta acggcaatac cggacaataa
    75781 gattcgattc atacccaaaa agccatttcg atcataggcc gcaagataga cacagcaagc
    75841 agctaaaaca ccacctagaa aggctacaac tagcaataga aacggcagcg aatagtgcga
    75901 aaagaaacct aaataaagca tcactaaaaa tcctgctccg gcattaatgc ccaatatact
    75961 agaatctgcc aattcatttc ttgttactcc ttgcaagata taaccagaaa cgccaagaca
    76021 agctcctgct aataaactga tcagtaatcg aggaactcga aattctctaa caatcagcgc
    76081 caaagaagat gattctttcc ctatgaacgt ggcaaaaagc tgatccaggt aaataaaagg
    76141 cgtaccaatc attaaactac ctatagacag gatgacaagt acaaaaagca agaaatagaa
    76201 cgtccaatat atttttttca ccgttgttcc ctccttactt gtatcagtaa aaacggaacg
    76261 ccaatcaatg caatgactaa tccaaaaggt gtttcaaaag ggggattgat catcctcgct
    76321 gtaaaatccg ccaacataac caatccaccg ccaactaaaa aagagacagg tagaacataa
    76381 cgatagtccg ttccaaccat tccgcgaaca actggcggaa ccaacagccc aataaatgaa
    76441 acaggtccga ctaacgaaac ggcaatggct gctaacactg caactagaca gaaaacaatc
    76501 aaccgcgttc gtttaggatg acttccaaga gctaatgcat gtgtttcact caaatgaatc
    76561 aaagatattg cttttcctaa tgaaaaagaa accaaaacag caagtatata taaaggaaac
    76621 aatactgcta attgtttcca tgtgatattg gcagctccgc ctacaaacca aaaagcaaga
    76681 tcctgctgca gattaaagaa ttgggtaagt gcttggctga tagcagtaaa aaaagaacta
    76741 atagctacgc cagctaaaat cagctgtaaa ggattcatgc ccattttcga aaactgcaaa
    76801 aaaccaaata cgagcaaagt tacagcaaaa gaacctgcca atgacattag aaacacagaa
    76861 aaatcagaag ctttaggcca aaaagcaaaa ctaagggcca ttgccaacga agcacccgcg
    76921 ttgattccta gtaagcctga atctgctaaa ggatttcttg tcactccttg catcaatgca
    76981 ccgctcaatg caaaacaact ccctacaaga aatgctcccg ccacacgagg caaacgaata
    77041 tttcgtatga tttggtgact ttgttttgat ctatcaaaat caaacaacgc ctgccaaaaa
    77101 tctgcttttt ccacagtcgc tgcgccattt gaaagagcaa caaaaaaaat aatcaaaaga
    77161 acaaaacaga ccaaaagcag gagtctattt ttcttcatag tcctcataat tgatcctcct
    77221 gctcttttgg caacaaatca taggttagaa aaactggttt atttgttctt ggaagcgtga
    77281 caatcgttgc atcaatctca aatatttttt tcatacactc ttttgtgatc acttcttcag
    77341 gtgtaccttc tgtgatcaac ttaccgtttt tcattgctaa taaataatct gaaaaacgtg
    77401 aagcatgatt aatgtcatga atcgacatca agatcgttgt ttgtttcatt tgattgattt
    77461 cagtcaataa ctgtaggatc tctaattggt gtgccggatc taaaaatgtt gtcggttcat
    77521 ccaagatcaa gatatctgtt tcctgagcaa gcgccatagc gatccagaca cgctgggttt
    77581 gtccaccaga cagcgttgcg atatcccgca tacgaaatcc agctagacct gtagcatcca
    77641 acgcccactc aattgcttca tgatcttgcg tgtccatgcc actgaacagt ttttgataag
    77701 ggtatcgtcc gtaagatact acttcttcta ctgtcatacc tgtagggtgt tcactagttt
    77761 gtgctaataa tgcaattttt tttgcaagtt cttttgaact gtatgtagag atatcagagt
    77821 tattgacaat gatctttcct gatttcacag gtagtatacg ggtaaaactt ttcaataaag
    77881 tagattttcc acttccattt ggcccaatca aactagtgat tttcccttca ggaatcgtaa
    77941 cagacatgtt ttcaatgatt tttttgtttt cgtagccggc tgcaatgtca gctgctctta
    78001 atctcgtcat ttcacccatc ctttcgctga aaatcattct caattatgac gctgtttcaa
    78061 catttcgtca agcgctactt tcatatctat agccaaatga agcggttgac agcgttcgaa
    78121 atatcaggaa taataaccaa tgagaaagat tctcaattat gttaggagga aagaaatgaa
    78181 attaaaaaca aaagtattca cattatctag catattgctg ctggctggag tactgagcgc
    78241 ttgctccccc tcttcagata aagaaacagc tacaactgcc aattcgacga cttcatccaa
    78301 tagtactcat acagtcacag atacacttgg acataaagta gagattccaa acgcgcccaa
    78361 acggatcatc ggcagttact tagaagacta tctgattgca ctaggggaaa aacctatcgc
    78421 ccagtggact gttggcagtg gttccattca agattatctg caagatgatt taaaggatgt
    78481 cccaacgatt tcttatgacc ttccttatga gaaagtgtta agctttgagc ctgatttgct
    78541 gttgatttct tcttccgcta ctgtcgaagg cgggaaatac gaacaatata gcaaaatcgc
    78601 gccaacttat gttgtaaaaa acggaacaga tgtcacatgg gaagaacaac taaaagatgt
    78661 cggcaaagct ttggcaaaag aaaaagaggc agaaaaaatc atttccgatt atcaaaaatc
    78721 catcaagaaa actcgagaag aactagcaga taaaatcgag aacaagacag ctgcagtgtt
    78781 gtgggtgacc aacaactcag cctttatggt cagcaaaaat cggtcaagtg gaagaatcgt
    78841 atacgacgat ctggaatttg gtgttcctga tctagtggaa gaggtctcca aaaaagccac
    78901 ttctgactgg tctgctgttt cctctgaaaa gctagctgaa ttagatgctg actacatcat
    78961 ccttgtgaac agtgacaaag atgctgccat gttcaatgaa ccaagctgga agaatctaaa
    79021 agcagtgaca gacaatcatg tactgcaatt cggacctgaa tctagttggc tatataacgg
    79081 acctattgcc agtcaggaaa tggtcaatga tatcaaagaa aaactaaatt gattttaact
    79141 aaaaaaagca tcttcacgat tagtcgtgag atgctttttt tcttattaac aaactagacg
    79201 catagatttt attcttcttt tgaagcaacc ctatttttta ctctttccca tttttgaaaa
    79261 cgatgggcat atagattttc ttgatccgtt atcccctcat actcctctat aagggcgaag
    79321 tctgaaaaat caatattccc aatatacgta tcgccttcaa acgtgctatc gatcaatgtt
    79381 cgccagatcc gaccagtgta aggaagaagt tcttgaaaaa tccgactacc acccgaaaca
    79441 tagatatctt ctgagatcgt ttgactatac gacaaaagtt catcgatcga atgcattact
    79501 tccgcatttt ctttcgtgcg aaaatttggt tgcgttgtca acacgatgat atggcgataa
    79561 ggaagtgaaa gatcccccat tccttcatag gtctttcttc ccatcactaa aattcgatcc
    79621 attgtatgtt cgcgaaagaa acgcatatca tttggcaatc gccatggtaa caaaccattt
    79681 ttgccgatca gaccattttt atcttgtgcc cacatggaaa taaacatatt atccgctcct
    79741 tatgatttta gatgttttga gataaaaaaa gtctcaattc tttgaccagc aaggaattga
    79801 gaactttaaa aacattattt tgtttcacgg tgcaaagtaa ctttgcgttc gcgtgggcaa
    79861 tatttttgtt tttccaaacg atcaggattg ttacgtttgt ttttgcttgt tagatagtta
    79921 cgttctttac aagaagtaca ctctaaagtg atgtttacgc gcatgttttt ccctcccatt
    79981 tttacaagaa tcctctaaga tgactctcag acttgattat cttaccatga ttttttctag
    80041 attgctagtg tttttcacga ttttgttaag taaaactact tgacgataaa tacaacggtt
    80101 ttacttctca aacaaaatct ttttcttttt gtataaatac ccttacactt gtttatatag
    80161 attatgtttt ttaataaagg gggatactaa tatgccatgg aacatgaagg actttcctgc
    80221 atcaatgaaa aacttagata aattgacaag aaaaaaagca atcgacatcg caaatgcatt
    80281 attagatgaa gggtaccctg ataaccgtgc gatcccaatt gccattgatc aagcaaaaaa
    80341 atggtatgaa gatgctagtg aatcagaaaa acgaactttc gaaaaagaaa aaaatccaag
    80401 caagacggat gaacacgata cgaatcctcg tgctggaaaa ctgcttgatt cagatgtgat
    80461 cgtagaatat gaggaagaac aatggatcgt gaaatctaaa ggggcaaaaa aggcaagcaa
    80521 ccattttgat acaaaaaaag aagcaatcga aaaaggaaag caagtagcac aaaacaaaga
    80581 atcaactttg ttcatttata aaaaagatgg gacaaaagaa aaagaaatcg attattgaac
    80641 atatgtaaag gggttgtgac gaaaatcgtg tcacaacccc cttatgttcg aaacaatggt
    80701 gttcctctgc caactccgaa ccataagtca taatatctat aaaacgggaa gagctgtttt
    80761 ttgaaattgt ttcttatcac tcagtgttaa acggtaggag aaaacctctt ttttattgat
    80821 atttgatttt catcaaacag tcacatagtc gattgcttct gcttgcggaa tcgtttcatc
    80881 tacaaagtag actttatacc atgtcaagaa actgaagatt gtccagattt ttctttgctc
    80941 ttctttacgt ccttcgtgat ggtcatctag caacttcatg attttttctt gatcaaaaaa
    81001 ttcttttgca aaatctgctt cgaataatgc tttgacttgt tcgtagccgt gttcttcttt
    81061 caaccaagct ttgattggca cagggaatcc taatttcaca cgattcgacc attcttccgg
    81121 aagatgtttg ttcgcagctt gtctgaaaat atcttttgtg ttgttttgat tcaataaaaa
    81181 cttacttggt atttgttgag ccaatttcat gacttcgata tccaataaag gtactcggac
    81241 ttccaaagaa cttgccatag aaagtttatc tgcctttaac aagatatctt ttggcatcca
    81301 ttgatgaaga tcgacatatt gcatcttatt gacttcatct ttgattcctt ctgttttctc
    81361 gtaatgaaca gtcatgattt catccacaga tggtgctgtt tgatattttg gtgttaacac
    81421 ttctgatgct tctgattctt cgaacactcg agcatgaccg ataaaatatt cttttgcagg
    81481 agcaagtgat tcatataaat ggatccgtcc atggaagttt ttcatttttc cgattttacg
    81541 tcctaaatta tagcgtgttc cttttggtaa ttttttcaat ccttgagcaa tcacacggat
    81601 gaattttgaa ttcgtatgga atccatacgt ttggtatccc gcaaacagtt catccgcccc
    81661 ttctccagat tggactaccc gcacactttg tgaagcaaga tttgctaaaa agtacaaagg
    81721 tacgcaagaa ggatttgaat caggttcatc taagtgatac tggatcaaag ggaatgcttt
    81781 gaacgacatg tctccgtcga tcactgctgc tgtattatct aagtccaata gttcggttag
    81841 ttttctggct tcaatcgctt cattataggt tttatcatca aacccgattg agaaagaatg
    81901 atctggtttc aatacagagg ttacataact ggaatcaacc ccgctggata agaaagaacc
    81961 aacttctaca tcactgaccg tatgtgcttc aatagaagct tgaacggttt cgtcgatttt
    82021 ttcgatccat tcttgtcggg aatgcgtttc ttttgtttca aaatctgcat cccaatattg
    82081 ctgaatgtct atttttccat ctttatacgt ataataatgg ccttcaggca aacgatagac
    82141 atctttgaag aatgtttcgc cgtttaatgg agaatattgg aaagtcatat aaggtttcaa
    82201 cgcttctttg ttcaattctt tgttaaaatc aggatgaggc agaaaacttt tgatttctga
    82261 accaaacata aatgttccgt tcatttctgc ataataatat ggtttgatcc caaagtggtc
    82321 tcttgcaccg aataactcat tcttttcatt atcccaaata gcaaaagcaa acattccgcg
    82381 gatcttttgc aataattctg ttccccattc ttcgtaacca tgcaaaagga cttctgtatc
    82441 tgcatgtgtt tgaaaaacgt ggccagctgc aatcaattct tctctcaaag gctggtaatt
    82501 atagatttcg ccattaaaaa taatgatctt tgtccgatct tcattgtaaa taggctgagt
    82561 accgccctct aagtcaataa tacttaatct tctgaatcct aaagcaacat gtttatcgat
    82621 atattctcct gaactattag gtccacggtg aacgattcga tccatcattg tattaataat
    82681 tgtttttttg ttgtccttgt cgttaacaaa acctacaata ccgcacattt aactttcaca
    82741 tcctaatata aaattactgt ttatttcatt catatgcatc ttatcataga ataaatagcg
    82801 gacgaatatt ttctctctta caatttcgtc acgtgaaaac aaaagatgaa agtttctgtt
    82861 tttacagaaa ataaagtccc aatatctaat ttaccatgtt ttcttttttg aggctacctt
    82921 attctcttcg tgttaaaaaa atattagtgg atgctatttc caaaaaaacc agcaaaaaaa
    82981 gacacgacct aaagatcgtg t
//
LOCUS       DANKEF010000006        77282 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000006 DANKEF010000000
VERSION     DANKEF010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 77282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 77282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..77282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.006"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            600..1289
                     /locus_tag="PWZ48_000454"
     CDS             600..1289
                     /locus_tag="PWZ48_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2260134.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1276..2484
                     /locus_tag="PWZ48_000455"
     CDS             1276..2484
                     /locus_tag="PWZ48_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2260135.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2778..4049
                     /gene="serS"
                     /locus_tag="PWZ48_000456"
     CDS             2778..4049
                     /gene="serS"
                     /locus_tag="PWZ48_000456"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDL2260136.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4598..6082)
                     /gene="guaB"
                     /locus_tag="PWZ48_000457"
     CDS             complement(4598..6082)
                     /gene="guaB"
                     /locus_tag="PWZ48_000457"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDL2260137.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6587..7282)
                     /locus_tag="PWZ48_000458"
     CDS             complement(6587..7282)
                     /locus_tag="PWZ48_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HDL2260138.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7372..8472)
                     /gene="ychF"
                     /locus_tag="PWZ48_000459"
     CDS             complement(7372..8472)
                     /gene="ychF"
                     /locus_tag="PWZ48_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDL2260139.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8481..8672)
                     /locus_tag="PWZ48_000460"
     CDS             complement(8481..8672)
                     /locus_tag="PWZ48_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HDL2260140.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8693..9583)
                     /locus_tag="PWZ48_000461"
     CDS             complement(8693..9583)
                     /locus_tag="PWZ48_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HDL2260141.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9570..10337)
                     /locus_tag="PWZ48_000462"
     CDS             complement(9570..10337)
                     /locus_tag="PWZ48_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2260142.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10349..11062)
                     /gene="rsmG"
                     /locus_tag="PWZ48_000463"
     CDS             complement(10349..11062)
                     /gene="rsmG"
                     /locus_tag="PWZ48_000463"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDL2260143.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11294..13195)
                     /gene="mnmG"
                     /locus_tag="PWZ48_000464"
     CDS             complement(11294..13195)
                     /gene="mnmG"
                     /locus_tag="PWZ48_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDL2260144.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13212..14609)
                     /gene="mnmE"
                     /locus_tag="PWZ48_000465"
     CDS             complement(13212..14609)
                     /gene="mnmE"
                     /locus_tag="PWZ48_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDL2260145.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14908..15291
                     /locus_tag="PWZ48_000466"
     CDS             14908..15291
                     /locus_tag="PWZ48_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260146.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15459..16217)
                     /locus_tag="PWZ48_000467"
     CDS             complement(15459..16217)
                     /locus_tag="PWZ48_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HDL2260147.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16235..17053)
                     /locus_tag="PWZ48_000468"
     CDS             complement(16235..17053)
                     /locus_tag="PWZ48_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2260148.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17050..17406)
                     /gene="rnpA"
                     /locus_tag="PWZ48_000469"
     CDS             complement(17050..17406)
                     /gene="rnpA"
                     /locus_tag="PWZ48_000469"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDL2260149.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17524..17658)
                     /gene="rpmH"
                     /locus_tag="PWZ48_000470"
     CDS             complement(17524..17658)
                     /gene="rpmH"
                     /locus_tag="PWZ48_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDL2260150.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18181..19515
                     /gene="dnaA"
                     /locus_tag="PWZ48_000471"
     CDS             18181..19515
                     /gene="dnaA"
                     /locus_tag="PWZ48_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDL2260151.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19713..20843
                     /gene="dnaN"
                     /locus_tag="PWZ48_000472"
     CDS             19713..20843
                     /gene="dnaN"
                     /locus_tag="PWZ48_000472"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDL2260152.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21072..21314
                     /gene="yaaA"
                     /locus_tag="PWZ48_000473"
     CDS             21072..21314
                     /gene="yaaA"
                     /locus_tag="PWZ48_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HDL2260153.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21301..22425
                     /gene="recF"
                     /locus_tag="PWZ48_000474"
     CDS             21301..22425
                     /gene="recF"
                     /locus_tag="PWZ48_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDL2260154.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22422..24368
                     /gene="gyrB"
                     /locus_tag="PWZ48_000475"
     CDS             22422..24368
                     /gene="gyrB"
                     /locus_tag="PWZ48_000475"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDL2260155.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24388..26859
                     /gene="gyrA"
                     /locus_tag="PWZ48_000476"
     CDS             24388..26859
                     /gene="gyrA"
                     /locus_tag="PWZ48_000476"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HDL2260156.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27095..27394
                     /locus_tag="PWZ48_000477"
     CDS             27095..27394
                     /locus_tag="PWZ48_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDL2260157.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27444..27956
                     /gene="ssb"
                     /locus_tag="PWZ48_000478"
     CDS             27444..27956
                     /gene="ssb"
                     /locus_tag="PWZ48_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2260158.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27982..28218
                     /locus_tag="PWZ48_000479"
     CDS             27982..28218
                     /locus_tag="PWZ48_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDL2260159.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28361..30334
                     /locus_tag="PWZ48_000480"
     CDS             28361..30334
                     /locus_tag="PWZ48_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HDL2260160.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30340..30792
                     /gene="rplI"
                     /locus_tag="PWZ48_000481"
     CDS             30340..30792
                     /gene="rplI"
                     /locus_tag="PWZ48_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDL2260161.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31066..32433
                     /gene="dnaB"
                     /locus_tag="PWZ48_000482"
     CDS             31066..32433
                     /gene="dnaB"
                     /locus_tag="PWZ48_000482"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDL2260162.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33213..34505
                     /locus_tag="PWZ48_000483"
     CDS             33213..34505
                     /locus_tag="PWZ48_000483"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDL2260163.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            35241..36104
                     /locus_tag="PWZ48_000484"
     CDS             35241..36104
                     /locus_tag="PWZ48_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2260164.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(36233..37855)
                     /locus_tag="PWZ48_000485"
     CDS             complement(36233..37855)
                     /locus_tag="PWZ48_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260165.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            38104..38625
                     /locus_tag="PWZ48_000486"
     CDS             38104..38625
                     /locus_tag="PWZ48_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2260166.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(38811..40541)
                     /gene="spxB"
                     /locus_tag="PWZ48_000487"
     CDS             complement(38811..40541)
                     /gene="spxB"
                     /locus_tag="PWZ48_000487"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HDL2260167.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            40706..41524
                     /locus_tag="PWZ48_000488"
     CDS             40706..41524
                     /locus_tag="PWZ48_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HDL2260168.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(41627..41824)
                     /locus_tag="PWZ48_000489"
     CDS             complement(41627..41824)
                     /locus_tag="PWZ48_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260169.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            41993..42571
                     /locus_tag="PWZ48_000490"
     CDS             41993..42571
                     /locus_tag="PWZ48_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2260170.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            42666..42803
                     /locus_tag="PWZ48_000491"
     CDS             42666..42803
                     /locus_tag="PWZ48_000491"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260171.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(43616..44119)
                     /locus_tag="PWZ48_000492"
     CDS             complement(43616..44119)
                     /locus_tag="PWZ48_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2260172.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            44478..45503
                     /locus_tag="PWZ48_000493"
     CDS             44478..45503
                     /locus_tag="PWZ48_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2260173.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            45850..46728
                     /locus_tag="PWZ48_000494"
     CDS             45850..46728
                     /locus_tag="PWZ48_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HDL2260174.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            47204..47320
                     /locus_tag="PWZ48_000495"
     CDS             47204..47320
                     /locus_tag="PWZ48_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HDL2260175.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            48248..48586
                     /locus_tag="PWZ48_000496"
     CDS             48248..48586
                     /locus_tag="PWZ48_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260176.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            48680..48910
                     /locus_tag="PWZ48_000497"
     CDS             48680..48910
                     /locus_tag="PWZ48_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260177.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            49310..49681
                     /locus_tag="PWZ48_000498"
     CDS             49310..49681
                     /locus_tag="PWZ48_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDL2260178.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(49955..50647)
                     /locus_tag="PWZ48_000499"
     CDS             complement(49955..50647)
                     /locus_tag="PWZ48_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2260179.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(50650..51435)
                     /locus_tag="PWZ48_000500"
     CDS             complement(50650..51435)
                     /locus_tag="PWZ48_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2260180.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            51672..53072
                     /gene="lacG"
                     /locus_tag="PWZ48_000501"
     CDS             51672..53072
                     /gene="lacG"
                     /locus_tag="PWZ48_000501"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2260181.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            53097..53414
                     /locus_tag="PWZ48_000502"
     CDS             53097..53414
                     /locus_tag="PWZ48_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2260182.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            53427..55100
                     /locus_tag="PWZ48_000503"
     CDS             53427..55100
                     /locus_tag="PWZ48_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HDL2260183.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            55114..56745
                     /locus_tag="PWZ48_000504"
     CDS             55114..56745
                     /locus_tag="PWZ48_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HDL2260184.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            57617..58807
                     /locus_tag="PWZ48_000505"
     CDS             57617..58807
                     /locus_tag="PWZ48_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISLgar5 family transposase"
                     /protein_id="HDL2260185.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGRELHDRYAAI
                     YMDATYIPLKRKTVAKEAIHIAVGIRPDGSKEVLSYAIAPTESITIWEEILLDLQERG
                     LKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRNISHKVRVDDRKEVCDDFKMV
                     YQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLLTFYDFPLAIRKSIYSTNLIE
                     SFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFLGRSHKGFQQAEGELEQMLSQ
                     LIEN"
     gene            58941..61130
                     /locus_tag="PWZ48_000506"
     CDS             58941..61130
                     /locus_tag="PWZ48_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2260186.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            61127..62920
                     /locus_tag="PWZ48_000507"
     CDS             61127..62920
                     /locus_tag="PWZ48_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2260187.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILIILKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLY
                     KFVTKHIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGI
                     TLTTAHKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNL
                     FLFSEVGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            63120..63434
                     /locus_tag="PWZ48_000508"
     CDS             63120..63434
                     /locus_tag="PWZ48_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2260188.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            63455..63832
                     /locus_tag="PWZ48_000509"
     CDS             63455..63832
                     /locus_tag="PWZ48_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2260189.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            63842..64615
                     /locus_tag="PWZ48_000510"
     CDS             63842..64615
                     /locus_tag="PWZ48_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260190.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            64637..66040
                     /locus_tag="PWZ48_000511"
     CDS             64637..66040
                     /locus_tag="PWZ48_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2260191.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            66222..67406
                     /locus_tag="PWZ48_000512"
     CDS             66222..67406
                     /locus_tag="PWZ48_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2260192.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            67403..67693
                     /locus_tag="PWZ48_000513"
     CDS             67403..67693
                     /locus_tag="PWZ48_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260193.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            67690..67911
                     /locus_tag="PWZ48_000514"
     CDS             67690..67911
                     /locus_tag="PWZ48_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260194.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            67953..68732
                     /locus_tag="PWZ48_000515"
     CDS             67953..68732
                     /locus_tag="PWZ48_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL2260195.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            68799..69293
                     /locus_tag="PWZ48_000516"
     CDS             68799..69293
                     /locus_tag="PWZ48_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2260196.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            69349..69987
                     /locus_tag="PWZ48_000517"
     CDS             69349..69987
                     /locus_tag="PWZ48_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260197.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            70059..70454
                     /locus_tag="PWZ48_000518"
     CDS             70059..70454
                     /locus_tag="PWZ48_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2260198.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            70438..72891
                     /locus_tag="PWZ48_000519"
     CDS             70438..72891
                     /locus_tag="PWZ48_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2260199.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            72888..74900
                     /locus_tag="PWZ48_000520"
     CDS             72888..74900
                     /locus_tag="PWZ48_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260200.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETKY
                     SKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSNATGYL
                     VKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYVAYTGL
                     VKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTKIVLPN
                     TDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDTNNGED
                     RENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFSQVLFI
                     IFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTMLYSLSA
                     SSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNRQTRRF
                     QRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQQGGIG
                     QRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDFKQSMA
                     DTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRKRQQTV
                     TPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            74897..75919
                     /locus_tag="PWZ48_000521"
     CDS             74897..75919
                     /locus_tag="PWZ48_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2260201.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            75936..76865
                     /locus_tag="PWZ48_000522"
     CDS             75936..76865
                     /locus_tag="PWZ48_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2260202.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            77110..77226
                     /locus_tag="PWZ48_000523"
     CDS             77110..77226
                     /locus_tag="PWZ48_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2260203.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT        24505 a        13811 c        15260 g        23706 t
ORIGIN      
        1 acttcatcag gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat
       61 cagagagaca agtaattgtc aactcttttt taaaagtttt ttcgatttcg tttttcgaaa
      121 tcttccagca actcatttat cataacttat cgtttgttat ttgtcaagaa cttttttgaa
      181 gtttttttcg atagaatcaa gcttcgcaag tggttctttt ttaacgactc agttatctta
      241 tcatgtcatt tccaacatgt caacaactta ttttttatta tttaataagt tattggtgtt
      301 cttttgaaca aataataata tatcaacatc acatgtgaaa gtcaataaga aaatgcttat
      361 ttatcaacat ttctagctta catgatcatt ggttagttct ggtccttttt cgttcgtctt
      421 tataactccg agagacgtaa aatccttttg ttttcagttt aatgttcatg ttcttgtctt
      481 attcacacca actagatgtc tcaccttcta aaattttcct taacaaagtg ctgcttttat
      541 ctatttctgc ttttttttgc tagaataata gtaacaatat gactctggag gatttattta
      601 tgaaaatttt gattgctgac gatgataaag aaatcgttga attattaagt atctatgttc
      661 acaatgaggg gtatgaagca gtaaaggctt atgatgggaa agaagcatta tctaaaatca
      721 gaacaatccc tgatattgaa ttacttattt tagatatcat gatgcctgag atggatggga
      781 tgcaagtagt gaaagagcta cgaaaagaat cccaaatacc aatcattatg ctgacagcaa
      841 aaactactga tatggataag atcaaaggac tggtcgctgg tgcagatgat tatgtgacca
      901 agccatttaa tccgttagaa gtaatggcta gagtaaagtc tttattacgg cgtacaaaaa
      961 tgcagattgc cccagatcag cctgatgtat tagaagtaaa tgcattaatc atcaataaag
     1021 actctcatga agttaaaaca atcgatggga aagaaatcca gttaacagct ttggaattcg
     1081 gtatcctcta cttgcttgct agtcatccta atcgagtatt tagcgcggat gaaatttttg
     1141 agcgtgtttg gaaacaagaa agtattgtct ctgctaaaac tgtcatggtt cacgttagtc
     1201 atttaagaga taagattgaa gaagcaactg gtggtgaaaa agtcattcaa accgtttggg
     1261 gagtgggcta taaaattgac gcaagatagt aaacgagcaa ctgaaaaacg ccgtcggatc
     1321 aatttgactt caaaagaaat cagcgaattg ttagcggaag gaatcatcac gattatcctt
     1381 ttgcttctat taaatgtttc tattctggtt gttatcagtt cggtgatcaa cagttcacct
     1441 tctttgacga atgcgatttg ggattcaaag aatatttttg cagaacgatt gaacactgat
     1501 ttgttctgga acggccggaa ttttattatt ccctttttct ttctattaga tattggtgtt
     1561 ttgtattggc gcttgattcg aagatatcgc caaatgcaat tgcggcatat catcagcgaa
     1621 cttcactaca tcgcaaacgg aaattatgat catcgtatcc cttttgaact gagtggtgac
     1681 ttaagccgag tggtaacaag tatcaatgga ttggtggata gtaccgtagc agcgattgaa
     1741 gacgaacgta aaatcgaaaa atcaaaagat gaattgatca cgaatgtcag tcatgatatc
     1801 cgtaccccac tcacgtccat cattggatac ctaggactga tagaagatgg ccagtatcac
     1861 tcagaagaag atcttttaaa gtatacgcat actgcttata tcaaagcaaa gcagatgaaa
     1921 tctttagtgg acgacttatt tgaatacact aaggtacgcc agcctgctgt cccggtgaat
     1981 ttttctgcct ttgacatgat ccagttgatc gaacagttag cagctgattt tgagttggaa
     2041 gcttctaaaa aaaatatcca aatacttgtt caatcaaaag tagactccct tatcatggat
     2101 ggcgataccg aaaaacttgt acgtgtcttc aataatcttt tgacgaatgc gttgaaatac
     2161 ggaaaaggag caacgaagat cgtaatcgaa gtagaacgta tcggttcaga agttgttgca
     2221 acagtcaaaa ataacggagc aatgatccca caacaggcaa tcgataatct attcgaccgt
     2281 ttttatcgtg tagaagaatc tcgttcacaa gcaactggag gaactggact tggtttagca
     2341 atcgctcaaa gcattgtggc tttgcacggc ggatacattt atgcaaaatc agataagcaa
     2401 tggacttctt tcatcatgca ccttccaatc aagaaaaatg aaaaattacc tattgataca
     2461 caggaagtta ttgacgaaag ctgaaatatt cgctattctt attacaatta ttagaataaa
     2521 tgaaaaacac agaacccagt acactttatt ctttttcaga gagctgatgg caggtgtaaa
     2581 tcagcaaagg ataatttgga agttccagtt cgtttacgtg ccctttcgaa ataagaaagg
     2641 tcggttcaac agccgttatg tgttcaacaa gtgcagctga cgctgaactt gggtggtacc
     2701 gcgaatttca gactgattcg tcccaagaat aatgggatgg atcagtcttt ttttattttc
     2761 aaaaaggagg aaatcatatg ttagatgtaa aaatgatgcg acaaaatatt gaagaagtac
     2821 aagaaaaatt gatgactcgg ggcgtaaaaa aagaaacatt agctgaattc atggaactag
     2881 atgaaaatcg tcgtaagcta ctagtcaaaa gtgaagaatt aaaaaaatat cgaaatgatg
     2941 tttctgctga gatcgcccaa ttgaagcgtg caaaagaaga tgcttctgct aaaatcgctg
     3001 aaatgaaaga agtcggtggc aacatcaaag cattggatac tgaaattgct gaagtggatg
     3061 aaaaattgaa acaaattgca actactttgc caaaccttcc ccatgattct gtgccagttg
     3121 gaacagatga agaggacaat gtagaaatcc gtcgttggag cgaaccacgt acatttgctt
     3181 ttgaaccaaa accacactgg gaaattgcag aaaatcttga tattttagat tttgaacgtg
     3241 gcgcaaaagt agccggtagc cgttttgtct attacaaagg attaggtgct cgattagaac
     3301 gtgctgtcta caacttcatg cttgatcagc atatttacga acacggctat actgaaatga
     3361 tcactcctta tatcgtcaac agtaaagcga tgtttggtac gggacaattt ccaaaattca
     3421 aagaagatgt tttccaattg gagaatactg atctaacatt aatcccaaca gctgaagtac
     3481 cattaactaa ctattataat gatgaaattt tagatggtgc agatttacct atttatttca
     3541 ctgcactaag ccctgctttt cgttctgaag ccggaagtgc tggtcgggat acacgaggac
     3601 tgattcgtct tcaccaattc aataaagtag aaatggtcaa attttcggat gtagagcatt
     3661 cttacgaaga attggaaaaa atgacagctg atgcagaaga tatcttgaaa aaattgcatt
     3721 tgccttatcg tgtgatggcc ttatcaactg gagacatggg cttttctgca gctaaaactt
     3781 atgacttaga agtatggata cctgcacaag atacttatcg tgaaatcagt tcttgttcta
     3841 actgtgaaga tttccaagca cgccgcgcaa tgatccgtta tcgcgataat gatggaaaaa
     3901 tccactatac tcatactctg aatggttcag gacttgcagt tggccgtaca gtagctgcca
     3961 ttttggaaaa ctaccaaaat gaagatggtt ctgtcacagt tccagaagcg ctcgttcctt
     4021 atatggggaa tctgaaagtt attaaataaa gaggctgtga aaaagccgct tagtttcgag
     4081 tattaagaaa aattttgtga aaatggcttt ccacattttt gcataaattg gacttaatac
     4141 cgagaaactg gcttttgaac accatttatc gagaggttgt gaaatcagcg aattaacctg
     4201 agtattagag aaaaaaatgg aaaaatagtc cctcatattt taccgtttgt tgaactaata
     4261 ccgagggtca gctgattgaa caccgtttag tggagaggtc gtaagcctac cccccagctc
     4321 ctagttacaa ggacaaattc aaaaaacagc tcttcatatt ttatgaatgt tgtacttgtg
     4381 attcggaggt ggcaagggaa cactgtttat tcaaaaagag gctgggacag aagcgtttaa
     4441 ctccaagaaa taagaagaaa ttcacgaaaa ttgcttttca aatttttgtg aatttcttct
     4501 tatttccgaa ggagttgctt ctacttccgc cgtttatacg tgtttagagc gtgagacaaa
     4561 agtgttcttt acttttgtcc cacgctcttt aatgttttta agattcaact gaatagtttg
     4621 gtgcttcttt tgtgatttgt acatcatgag ggtgtgattc ttttaatcca ctgccgctca
     4681 tttggataaa ttgcgcttca tcacgtaatt gctgtagatt ggctgcccca acataaccca
     4741 ttcctgaacg caaaccgccg atcatttgga aaataatatc tgcaacactt cctttataag
     4801 caacccgacc ttcgattcct tctggtacta atttatttgc ttcattgaca ctaccttgga
     4861 agtaacggtc ttttgaacct ttttccatcg cgcctaatga gcccatacct cggtatgttt
     4921 tgaatcgacg tccttggaag atttcgaatt cacctggtga ttcatctgtc cctgccaaca
     4981 tacttcctag catcactgca tgtccgccgg cagctaatgc tttgacgatg tcaccagaat
     5041 atttgatccc tccgtctgca ataatcgctt tgccatattg acgagcaact gatgctgcat
     5101 cataaatagc tgtgagctga ggtacaccta caccggctac cacacgtgtc gtacaaatcg
     5161 atcctggtcc gataccaact tttacgacat ctacacctgc atcatataaa gcttttgtcg
     5221 cttcagccgt cgcaacgttt cctgcaatca atgttgcatc tgcaaaagtt gaacggattt
     5281 cttgaatttt tcggatcaca cctgcactat gtccatgagc tgtatcgatg atgattgcat
     5341 ctgcccctgc ttctaataaa gcattcgcac gctcaaatgt atcgcttgtt acaccaacgg
     5401 ctgctgctac aagcaatcga ccatgttcat cttttgctgc atttggaaat tcgatcactt
     5461 tctcgatgtc tttgatcgtg atcaatccgc ttaaacgacc ttcgttatct acgattggta
     5521 atttttcgat tttatgtttt tgcaaaatct tttctgcatc ttttagagag gtacctactg
     5581 gggcagtcac taagttgtct tttgtcatca catcagcaat cggcatgtga taatctgtta
     5641 caaaacgcat gtcgcggttt gtaattatcc ctaccaattt tctgttttca agtgtttcaa
     5701 cgattggcac accactgata cgatatttgc tcatcaattc ttctgcgtct gctacaagat
     5761 gctgtggcgt taagaagaac ggatcaatga ttacgccact ttcagaacgt ttgacttttc
     5821 ttacttcatc cgcttgctgg ctgatcgtca tgtttttatg gatcacacct agtccgcctt
     5881 gtcttgccat agcaatggcc atcttgctgt cggtaacagt atccatactt gcactgatga
     5941 ttggaatatt caacttaata tttttagcta attgtacgct catatccact tcgtttggta
     6001 acacgtgact ttctgctggt atcaataaga catcatcaaa agtaaatcct tttttcgtga
     6061 actttgtttc ccagttggac atttttagta ccactcctct aatttagttt atctattaaa
     6121 ataattgatt ttttcattta agtcaatgat ctacttggaa tcaagttagc tttctcatag
     6181 aataccaata taaagcgttt acatctgtgt atattaaaag agattgtaaa aaagctgtcc
     6241 tgcatcaagt aataagaaca gccaatcaaa aacagcttct catgtttttg aattggttgg
     6301 gtttattacc gcagatgcaa acttttgaac accgtttagt ggagaggctg tgaaaaagct
     6361 gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttccacatt tttgcataaa
     6421 ttggacttaa taccgagaaa ctggcttttg aacaccgttt atcgtaaaaa gggactgtga
     6481 caagactttt gtcacagtcc cttactatta tttaactatg gataaacaaa atgaggttct
     6541 ttactacccc tagatatcta gcaaaatagc tgatcttttt gctagtttat cttcttggga
     6601 cggataaact tccaataatc ccgtattttt tcttcaagta gtaacggatt gctaaagcag
     6661 ttgccccgat aatgataact actactggat ctaatactgg attgataact ggtggcaata
     6721 gtgttgtcgc agaaaacagt acgacccaaa gaagtgtcgt tccacctagg ataagcaatg
     6781 tcttacccat tcctggtttc ttgcttcgat ctgctccggg atattcatat tgatagatgt
     6841 atttgtacat catataaaag acccagccac cgattgcaga acctagaacg agtgtagaaa
     6901 agccataagc tggcgttgtt ccacgtgaaa acaatccggt aatcgcaatc atgatactta
     6961 atagaccaag caacagcaac gtattatcta accacatcaa taaaggggtg gatgaatttt
     7021 ttacttctgc tggcttattg atgattgctt ccgttctttc agagactgtc ccaaacaatt
     7081 gacgggctgt tcttccgcct ttttgttctg caactaaagt aggcagaatg tcatgaaggg
     7141 cttttacttt ttcttcttca gacaaattcg ctgcttctaa agattttttc aaatcaaaaa
     7201 tgtactgttg atttcttttg gttaattttg tttctaaact attgttttct tttacgattg
     7261 ctcgtaattg ttctggttcc attatctaat agtcctcctt agccgttcct tttctcaacg
     7321 gtcgtcacaa aagacattct accatattct acagctagtt tgatagccct tttacacatt
     7381 aaagcggaat aacattacgt cgccgtcttg tacgacataa tcttttcctt ctaatcgaac
     7441 acgtccagct tcttttgctg catgcatgtt tccgtaatga tccaagtctt caaaagagac
     7501 agtttctgca cggataaatc cgcgttcgaa atctgaatgg atgattcctg ctgcttgtgg
     7561 tgctttgatt ccttttttga atgtccaagc acgtacttct tgttccccag cagtaaagta
     7621 agtagctaag cctaacaaat cataagcagc acggatcaac tgatccaaac ctgattcttc
     7681 gattcctaaa gcttctaaaa attccgcttt gtcttcttca tctaattctg cgatttcttc
     7741 ttctgcacga gcactgacca cgatcacttc tgcattttct tctgcagcaa agttgcgcac
     7801 ttcttgtaca taatgattcg cgtctgcatc tgctacgtca tcttctgaga cattggctac
     7861 ataaagaatt ggttttgtcg tcaataaaaa tagtgattta acgatttttt gttcttcttc
     7921 agtaaattcg atcgttcggg cagattttcc ttcttccagt acaggtttga ttttatccag
     7981 tacagctagt tctgccactg catctttatc tttggtttta gcaacttttg ctacacgagt
     8041 atagcgtttg ttgatagatt ctaagtctgc taacactagt tccaagttga tcgtatcaat
     8101 atcttctagt ggatcaacac gtccttctac gtgagtaata ttatcatcat caaaacagcg
     8161 aacaacgtga caaatagcgt ctacttggcg gatatggctt aagaattgat ttcccaaacc
     8221 ttctccttta cttgcgcctt ttacgattcc agcaatatct gtaaattcaa aagtcgtcgg
     8281 aacagttttt ttcggatgta caagttctgt taagcgttgc aaacgccagt caggtacttc
     8341 taccatccct acgttggggt cgatcgtcgc aaagggatag tttgctgctt ctgctcctgc
     8401 ttttgtgatc gcattaaaca aagttgattt tcctacgttg ggcaacccaa caattccagc
     8461 agtcaatgcc ataaatttat tcactctttc tctgcatttt tttcaattac tttttttaat
     8521 ttcttttcaa aatctcgacg ggtcatcatg acgatatgtc cgcaattcat acatctgatt
     8581 ttgatatctg cgcccattcg aatgacttcc cagcgattcg tctggcaagc gtgaggtttt
     8641 ttcatttcaa cgatatcgtg caagtcgtac ataaaaatag cctccttttt gtttattctt
     8701 catcaaattg aatattcaag atatcaagaa ttcgagtcaa atctttttga gaaagatatt
     8761 caatctcgat cttacctttc ccattttttt cttggatagc tacacttgta ccaaatttat
     8821 ccatcaaacg atcttcgctt tctcggatat aatatggttt ttcctttgct agccttggga
     8881 tttttttctt ttcttttcca tcattttgat ttaactcaga aacaagctgt tccagttggc
     8941 ggacagttaa attttcagtt actgcacgat tggctaattt caagatcaat gttttatttt
     9001 ttaaacctaa cagcgtacgg gcttgtccca tagataaacg ctgatcttgt accatttcct
     9061 tgacaagatc cggtaaagaa agcaagcgca aataattggc gatataaggt ctgctctttc
     9121 ctaaacgact agctacttct gcttgtgtta gtttcaggtt tttcatcagc atctcatatg
     9181 cttctgcttc ttctaacgga ttcagatctt ctcgctgcaa gttttccagc actgctactt
     9241 gcatcatcgc ttcttcgtca aagtctctaa cgatagcagg gatcgtttct ttttcagcta
     9301 gtttggacgc tcgaaaacgt cgctcccctg caataatctc atatcctttt actgcggaac
     9361 ggcgtacgat gatgggttga aagacacctg attgttcaat agaattagct agttcctgca
     9421 aagatacttc ttcaaatgtt ttacggggct gatacggatt tggtcgcagc tcattcaatg
     9481 gtagctggac aacggtatca tcttttacat ccaccgtctc taagttttct aaatcttgaa
     9541 acagtgcatc gattcctcgg cctaatcctt tacttttctt cacgacttac cacttccttt
     9601 gccaatgctt ggtagacttc agctcctcga gaacgtggat cgtagtcgat gatcggtttt
     9661 ccatgactag gtgcttctga taaacgaata tttcttggaa tgatcgtttc gtaaactttt
     9721 tcgcggaagt atttacgtac ttcttctacg acttcatttc ccagatttgt tcgtgcatca
     9781 tacattgtca gtaagactcc ctcgatctct aattctggat taaagtgttt ttggactaaa
     9841 cgaaccgtat tcagtaattg gcttaatcct tccaatgcgt aatactcaca ctgaacaggt
     9901 atcaaaatcg aatcacttgc tgtaaatgaa ttgattgtca gatggccaag agaaggcggg
     9961 caatcgatca aaatataatc atactgactg cttacttctg ctaaagatcc ttttaatctt
    10021 gattctcttg ccatcattga cgtcaattcg atctctgctc ctgctaattg gagagtcgca
    10081 ggcacaatac tcaaattttc gtgttcagtg atcaaagttg cctcatcaat tggcaattca
    10141 ttgactagaa catcatagat gtctcttgtc acatcaggtt ttctgatccc gacaccactg
    10201 gtagcatttc cttgtgcatc catatccact agcaatactt ttttgccaag gctagcaaga
    10261 caagcaccta gattcaccgt cgtcgttgtt ttgccgacgc cacctttttg gttcgcaaca
    10321 gatattattt gtgccatctc ttctcctcct atttgattgg ctgcttatta ggcagtccgg
    10381 gctttcttgg gtatttcttt ggggttggtt tctttttttg aataacgatg atatgccgtt
    10441 gatcagagac attcggcaag gtaaccgcct cttctttgag gaatttacca cctaataaag
    10501 caatcgcttg ttttgcttct gtcaattcct cttcactttt tgctgctttc aatgcataaa
    10561 aatagccttc ttgtttagcc aagggcaaac acaattccgc taacacattc aaacgagcaa
    10621 ccgcacgagc agtgacaagg tcaaatgatg cacgaaagtg aggattttgc ccaaatgttt
    10681 ctgcccggtc atgatataag gaagcatcga ttcctaataa tcgggttaac tcagataaga
    10741 aggttattcg tttattcaga gaatcaacga tcgttatctg cagttctgga aaggcaattt
    10801 ttaaaggtat gctaggaaac cctgcccccg aaccaacatc gcataaagat aaaggttgcg
    10861 taaagtctac ttcaaatgct aatgtaagtg aatcgtaaaa atgcttcaga tatacgtctt
    10921 tttcatccgt aatagctgta aggttcatct tttcgttcca ttcgactaat gtccgataat
    10981 aaatagcgaa ttgattcatt tgatgatcgg taagctctat ccctttttct gccaaggatt
    11041 ggcgaaattc ttctggtatc ataaatattc ctttcattgt gaaacagcaa ttgtttcaca
    11101 gattctctcc ctactttaac gcaaaatctc tagacatgca atccttccat atggattctt
    11161 aaaaatttga aaagtaagaa aagccgatag actttttctt tctagtatga ctcataaaat
    11221 atacacagaa gagagaacta gctttgagca atttctgcaa aaactagttc tctcttctat
    11281 ttattattac gtattacgct ggttcactga ctttcgcaat cttcccttgt tcgatataga
    11341 ccattaggat acttaggtct gcagggttta cgccactgat tcggctagct tgggcaattg
    11401 tttctggttg gattttctgc aatttttgtt ttgcttctgt tgctaatccg ttgattgctg
    11461 cgtagtcgat gttttctgga atctttttcg cttccatgcg ttttaatttt tctacttttt
    11521 ccatggcttt tttgatgtag ccttcatatt tgatttggat ttctacttgt tcgatttctt
    11581 ttcgtgtcaa ttcttcattt tctggaataa accgtagaag ttctgcataa gtaatctcag
    11641 gacgtcttaa aaagtcgctt gctagtacac catcttttaa tggtgccgca ttaatcgttg
    11701 ctaaaaagtc ttggacttct tctgttggtt tgattcgtac attcatcaaa cgtttgattt
    11761 cttgttcaac tgcttctttt ttcttcaaat aattcttgta ttgatcttct tttactaagc
    11821 ctacacggtg acccatttct gttaatcgca aatctgcatt atcatgacgt aaaagcagac
    11881 ggtattcagc acgtgaagtc agcaaacgat aaggttcgtt cgttcctttt gttaccaaat
    11941 catcgatcat gacaccgatg taaccgtcac ttcgtttgat cactaaaggt tcttttccct
    12001 gaactttcaa agctgcattg ataccggcaa tcagcccttg tccagccgct tcttcataac
    12061 cgcttgtacc atttgtttgt ccagctgtat aaagattttc aacgacttta gtttctaatg
    12121 tcggacggag ctggtgtggc tctactacgt catattcgat agcatatcct gtacgcatca
    12181 tttctacttt ttctaatcca gcaatagaat gaagcatgtt gacttgaaca tcctctggta
    12241 aagaagtaga taaaccttga acatagactt cttccgtatt taagccttct ggttccaaaa
    12301 ataattgatg acgtggtttg tctgcaaaac gcacgatctt atcttcgatg gatggacaat
    12361 agcgagcgcc taccccttca acgatccctg taaacattgg ggcacgatgt aagttgtctc
    12421 gaataatttg gtgagtcgtc tcacctgtat aggtcagcca gcaagattcc tgatctagtt
    12481 tataggcact atctggtgtg ctgaagctaa aatgattcgg ctctttgtca ccaggctgct
    12541 cttctgtaac agaatagtcg atcgtacttg attttactcg aggcggtgtt cctgttttga
    12601 accgttcgat agcaaagcca agttctttta gatgattcgc tagtccaata gaaggttgag
    12661 agttattcgg acctgatgaa tatttcaatt caccgataat gatttctccg cgaagtgctg
    12721 ttccggctgt aataacaact gctttggccc ggtagctcgc tcctgtggac gtcactactc
    12781 catgacaaac accatcttcg acgatcagtt cttcgacgat tccttgtctt agcgtcaaat
    12841 ttggttcttt ttcgatcgta tgtttcattt ctgttgaata agcatgtttg tctgcttgtg
    12901 ctcgcaatgc tcggacagct gggccttttc ctgtgttcaa catacgcatt tggatatagg
    12961 ttttatcgat attcttaccc atttctccgc ctaacgcatc gatttctcga acgacaacgc
    13021 ctttagctgg tccgcctaca gaaggattac atggcatgaa tgctaccatg tccaaattta
    13081 ttgtcagtaa taatgttttt gatcccatac gtgcagcagc aagtgcagct tctgaaccgg
    13141 cgtgtcctgc gccaacaacg atcacgtcat acgcatctgc ttcataatgt tccaatcgtt
    13201 tgttcctcct tttattttcc taaacaaaat tgactgaata attgagtgat tagctcatct
    13261 tgtacactgt cgccgacaac ttctcctaga tagtcccagc aacgcgtcat atctatttga
    13321 acgagatcca caggcattcc tgaatcaata cctgcaatga cttcttcaag tgagagggaa
    13381 gctttttcta acaacgcgat atggcgggta ttagaaacat aagtagcgtc tctttctcct
    13441 gtttctccgc tgaagaataa atcagaaata gctgattcca aacgatctag tccgtcgttt
    13501 ttagctacgg aaattgaaaa aactggttca ttttcgatca atttcttcaa tttttcttgt
    13561 tccagctgtg ctggcaaatc tgttttattt agaagaatga tccgtttcaa tccacttgtc
    13621 gcttccaata attgttcatc ttctgctgtc agtggttcgc tttggtttag aacaagtaag
    13681 atcaagtctg cttctgctaa tgcttttcgg cttctttcga ccccgatccg ttccaccaca
    13741 tcttctgttt cccgaattcc agctgtatcg attagcttga gcggtacccc ccgaacattg
    13801 acatactctt cgattacatc tcgagtcgtt cctgcaatat ccgtaacgat tgctttttct
    13861 tctcttaaca aatggtttaa caaacttgat ttgccgacat ttggccggcc gatgattgcc
    13921 gtacttaacc cttcccgcag aaccttccct tgcttggatg tagcaagtag cgcttggatt
    13981 ctttgttgga tcatctgcgc tttttcaagc aataacttcg tagtcaattc ttcgacatca
    14041 tcatattctg gataatcaat atttacttct acttgtgcta atgtctctaa aatctcttgt
    14101 ctcaatgagc ggatcaaagc agacagattg ccgtctaact gattaagagc tagtcccatt
    14161 gctttgtctg tttttgcccg aatcaaatcc atcacagctt ctgcctgtga aaggtcaaca
    14221 cgaccattca agaatgctcg ttttgtgaat tcgcctggct cagctaatct tgcaccttct
    14281 cttaacaata gctgcaaaat ttgattgact acaacgatcc caccgtgaca attgatttct
    14341 actacatctt cccgcgtaaa tgtctttggt gctctcatca cggagaccat gacttcatcg
    14401 acaagctgat tgctcttggg atcaacgata tgtccataat ggatcgtatg acttggtact
    14461 tctgataatc gtttgtttcc agattgataa actttatctg ctagttgcac agcttgatcg
    14521 ccacttaaac gaacgatact gattgcacct tctccaggtg gggtagaaat tgccgcaatc
    14581 gtatcaaatt ctaaagtaat ttcagccatt ttttcacttc ctttttcatt ttgcgcacaa
    14641 aaaaagtgcc cactccaccc tttatgcaca ataaacggtg aacttcgcac tttgactgct
    14701 tgttcttgat taaattctgg tcatagatta tcacacttgc tataaggaat caagtgttta
    14761 aaacaattag atgaagttga gcctgttctg cttccattac cttatattta attgtccttg
    14821 gatgtctctt tgcgttcttt ggtttattcg aagagaagat actgtataaa caaggcttta
    14881 tctttcttaa aaaaaacggt acactaaatg gtagcgctat ctaaactata cacttttgaa
    14941 aggagaaaat atgtgagccc gttcagatct gctgcattgg aagaagaaag tgaccctcgg
    15001 atcaacgatg tcgttttatc tacagggaat atcaaccaga aatatatcgt ccgcgatatc
    15061 gtttttgttg tggaatgctt agaagctgat ttgtttgacc catcttttcc caaagatgac
    15121 cttttcttaa caacgaagag gaaattaaag acgaaggtac ttgaatatgg tgcgaatgca
    15181 gtaatcaact gtcattttga gtatcagcaa aaaaaacgcg acggaaagta ttttctggaa
    15241 ctttttgctt atggaacagt gatccaattt atccaaacaa cgattggtta aatgaaacag
    15301 acaaaaaaag cggatcaaaa ctgaagacag tttcgatccg cttttttcta tcaaagttag
    15361 tgactacttc atcgtatact ctgataagaa tgatttctag taaaatatac ttggtttgaa
    15421 aaactttgtc aacgtgattt attcaaaaag taatggaatc aataatattt tttagcaggt
    15481 tctactacta aataacgata aggttcctca ccttccgaat gtgtttttac atgttcatct
    15541 ttgcttaatg cagcatggat ttgtttacgt tcaaatgcag gcattggttc taagaaaaca
    15601 ggctgccctg tccgttttac tttttcagcc gtgcgatcag ctaaacgctg aatgatctct
    15661 tggcgctttt cacggtaatt gcctacattg actactacag acagcttatt tcttgctaaa
    15721 cgatggatga agacttgtgc cagatattgc aatgcattca gtgtttttcc atgtttccca
    15781 atcagtatgc cttgcttttg tgtttctaaa tggaaaacaa ccatattatt tgattctctt
    15841 tccattttga caagagctgg agcatcaagc tgttttgaaa tttcagtaag ataaactgcc
    15901 aattccgtta aagctgcttc atcacctagc tcacttactt cttctgtttt ttcttcaaga
    15961 atcgtttcgt tgactacctc gatctcagct gttggttctt cttccaaaat ttcttctaca
    16021 gtctgttcta ctgtttcaga aactttttcg ctgatacttg gctggatcga aagccgtgca
    16081 ttctttcgac ccattcctag gaaacctttt tttccttctt ccagtatttc gatttcgact
    16141 tggtctttgg ccaaacccaa atcagccaga cctagttgga cagcttcgtc tattgttgca
    16201 gcttcataaa tcggcattag cattacctcc cctattattt ccgttttttc ttaggattca
    16261 ttgctttctt aagagcacgt tctctttcgc ggattctccg tgcttcttct tcacgctctt
    16321 ttcgaatttt gaaagggttg ttgatcagca gcgtttgtac aacttggaag gcattggaaa
    16381 ctacccagta taatgaaaga ccgctggcta aattgacacc catcactaaa atcattacag
    16441 gcatcacgaa attcatgatc ttcattgatg cattcgattc aatttgactc atgctagata
    16501 aatacgtact tgcaaaagta aagattgctg ctaaaatcgg caaaataaat gttggatccg
    16561 cgttccctaa gttcaaccat aagaaatgcc cttgtttcaa ctctggtaca cgtgaaatag
    16621 attgccataa cgccatcaag attggcattt gtactaataa aggaagacaa ccagcatatg
    16681 gattgacgcc attttctgca tataaacgct gctgctcttc tcttaactta ctttgtgttt
    16741 ccggatctct tgaactatat tgttgctgca gtgcttttaa tttcggttgt aattcttgcg
    16801 ttttacgcat gctctttgtt tggaaatgca tcaacggcaa taggatcacg cggatgatca
    16861 gtgtaaataa aatgatccct atccctgcat taccaaatga caacgccttg atcgcctcag
    16921 caaagtaata aacaatataa cgatcccaaa ttcctgtact ctgtgcgctg acttccccag
    16981 ttccgcaacc ggataagacg atgaccaaac taacgatacc ggccatcaaa agtaaacgtt
    17041 tgtatttctt cacttactct tttcctctct gtcgattatt tttgcaagtt ttaagacgtg
    17101 gactaaattg gagcgaacct catcataagt caaccccttc acacttggtc tagcaatcac
    17161 aataaaatcc aactccggat caatttcttc ttttatagaa taaactgctg ctcggatttt
    17221 tcgtttgact tcattgcggt ttaccgcgtt gccgaccttt tttcctacag ataaaccaac
    17281 tcgaaaatga ggctgtcccg gtttttccaa acggtaaacg acgaatttgc ggttagcaaa
    17341 agaatctccc gtatggaaca cggcttgaaa ctctcgttct ttttttactc gatacgcttt
    17401 tctcattagt tttctttcct tttctatctc tcacttacgc tcataatcat accataatta
    17461 cgattgtcct ttcatcattt ccctaaaaaa gagtataaaa aaaaccactg aaaccccagt
    17521 ggcctaagct gaaagaactt ttcttccttt gcgacgacgg ctagctaaca cacgacgtcc
    17581 atttttagta ctcatacgtt tacggaatcc gtgaactttt tgacgtttac gtttatttgg
    17641 ttggtatgtt cttttcatat attccacctc caaaatgttc ttatctattt tataaacaca
    17701 gacatacttt gatagtataa cgacaaagta gtgattttgt caatataaaa tagaagatcg
    17761 attgttaaaa cttatccaca agattatttt tttctatggg aatcctatgt ggaaaaagtt
    17821 atgcacaggg attatccaca gataataaaa ggctggtttt acacagaaaa cacacgtgta
    17881 aaaaaagtaa tccacaatca ggcgatttcc tgtgtattcc gtctcctaac tattggtata
    17941 tctgtatttt ttatccacag acgcaagagg aaatctattg gtttttacga gttttccacc
    18001 tgtgcattag tggtgtggaa aacttttttt gacaactgtg tttttctctt ttttcttttt
    18061 gtcgattgtg tggataattg taattaaaaa atttttttct tcggtttctg tggaaaacta
    18121 ttgaaaaaaa tgctaaatta gtactagtta ttcacttttt aatgaaggag gaggccttat
    18181 atggtatccc tcgatgcttt atggaatgaa ttaaaagcaa cataccaaaa ggatttatcg
    18241 cctgctagct ataatacatg gatcgaaaca gcgaaccctc gaacccttga tcaaaaccag
    18301 ctcgttgttg aggttcctag caagattcat aaagaatatt gggaaaaaaa cctggcgaca
    18361 aaaatcgttg aagtcggtta tatgttatca ggaaatgaga ttatccctcg ctttattact
    18421 ggcgaagaag cagagcaaga agaagttata gaagaaaaaa atccaaaagt tgtggcacct
    18481 agtccgctaa aaaaagccat gctgaaccct aaatatacct ttgatacgtt tgtcattggt
    18541 aaaggaaacc agatggctca tgcagcagcg ctcgttgtag cagaagatcc aggttctatt
    18601 tataacccgc tcttctttta tggaggcgtg ggacttggta agactcactt gatgcatgca
    18661 attggtcatc aaatgctgca aagtcagcct aatgccaaag taaaatatgt aagcagtgaa
    18721 acttttgcta atgatttcat caattcgatc caaaacaaaa cagcagaaga gttccgtcaa
    18781 gaatatcgaa atgttgattt actgctagtg gatgacattc aattttttgc agaaaaagaa
    18841 gctacgcaag aagagttttt ccatactttc aatgccttgt acaatgaagg gaaacaaatc
    18901 gtactaacca gtgatcggtt gccaaacgag attccgaaac tgcaagaacg ccttgtttct
    18961 cgctttgcat gggggctgtc tgttgatatt acaccgccgg atcttgaaac aagaacagcg
    19021 attttacgta aaaaagcggc ggctgaacgt ctagagatcc cagatgacac actaagctac
    19081 atcgcaggac aaattgattc taacattcgt gaattagaag gtgcgcttgt tcgagtgcaa
    19141 gcttttgctg cgatgaatag tgaagatatt tcgactagtc tagctgcaga cgcactgaaa
    19201 acgttaaaat caggaaaagg acatcctcaa ttgtcaatcc tgcagatcca agaagaagtg
    19261 gcaaagtatt atcacatcca attgaaagat ctcaaaggaa agaaacgcgt caaatctatt
    19321 gttgttccta gacaaatcgc tatgtatttg gcacgtgagc tcacagataa ttctttaccg
    19381 aaaatcggag ctgaattcgg tggcaaggat catacaacgg ttatccatgc tcatgaaaaa
    19441 atccagcagc tgatggattc tagtgtcagt atgcaaaatg aagtatcaga aatcaaaaac
    19501 tttttactaa attaatttgt cgattctatc aactgaaaat agcatgtgga taaaagaaaa
    19561 aaacgttcaa aagttataca cactttttcc acagctgttt tttcgtttct ctcttacgtt
    19621 ttttgagttt tccacagaat caacatgccc tattactttt attattcttt ttaactaata
    19681 ataaataaac aaacattcaa aggagataca tcatgaaagt tactttaaac cgagctagct
    19741 ttatgcagga attgcaaact gttcaacgag ctatttcaag caaaaccacg atccctattt
    19801 tgacaggtgt aaaaatcaca ctgacacaag aaggtttgac tttgacgggg agcaacgctg
    19861 atatatcaat tgaaactttt ttgtctgttg aaaacgaaaa agcaaatatg caaatcgaat
    19921 ctactggttc cattgtttta caagcacgtt tctttagcga aatcattcgg agacttcctg
    19981 aagaaacatt tactttagaa gttttagaaa ataaacaagt agcgatcact tctggaaaag
    20041 cgaattttat cgtaaatgga ttagatgcag ataactatcc tcatcttcct gttgtcgaaa
    20101 gccataacca gatgaaatta cctgtacacg tattgactaa actaatcaac gaaacagttt
    20161 ttgctgtctc acaacatgag agtcgtccaa tcttgacagg tgtgcatttc attttatctg
    20221 ataattcttt attagctgta gctactgatt ctcaccgtct aagtcaacgc gtgattccag
    20281 tagaacaagc ggctgatcat tttgatattg ttattcctgg aaaaagtttg atcgaattat
    20341 ctcgctcatt aacaaatgaa gaagaaatcg tcgaaatcag cattatggaa aaccaagtgt
    20401 tgttcaaaac agaaacgatg tatttctatt cccgtttgct agaaggaaac tatccagata
    20461 ccaatcgttt gattccttca agttttaata cagaggttga attttctgtt cctagctttt
    20521 tagcggcgat cgaacgggct tctttacttt ctcatgaagg ccgtaacaac atcgttcgtt
    20581 tatctattcg tccagatgcg gttgttttat acggaaattc gccagaaatc gggaaagtag
    20641 aagaaagctt gagttataca gcaagcagtg gcgatccatt agatatttct ttcaatccgg
    20701 attatatgaa agcagcgttg cgtgcatttg gagatatgag catcaaagtg aaatttatct
    20761 ctgctattcg tccatttaca ttggaaccaa cggaagatgg cgttcaattt attcagctga
    20821 ttactcctgt acgtacaaat taacaatgat tttcaatgtt cttgaaaatt taattgaaaa
    20881 gaaatagaaa aaataaaatt ttaaacagaa ggccctaaaa tctcttttaa ggctttctgt
    20941 ttatttttgt ttaaatcacc ttttttagat taaatcgtct tatatggaaa aatatatgga
    21001 taaatttgtt ttattgcgaa aaaaaaggta taatagagta tacttttaat aatgaaaatg
    21061 agggatgggc attgaaacaa caagtcattc taaatacgga attcatgacg ttgggccaaa
    21121 tgttgaaaga agtcacagtt atcggtagtg gcggacaagc aaaatggtat ttggcagaaa
    21181 acactgtgtt agtcgatggc gaaccagaaa atcgtcgtgg acgcaaactt tatcccggca
    21241 tgatggttga agttccagaa gtaggaactt tttttatggt aaaaaaagga aacgacgata
    21301 atgaggctga ataagctgta tttaaaaaat tatcgaaatt acgaagagct gaacatcgaa
    21361 ttttccaaga acctcgtcat ctttttagga gaaaatgcgc aaggaaaaac gaatcttttg
    21421 gaaagtatct acgtattagc tatgacgcga agtcatcgga caagtagtga gcaggaattg
    21481 attggctgga ctgacgatca ggcgatgatt caaggagaga tcacaaaagg ttcatccact
    21541 cttccattag aaatcttatt atctaaaaaa ggccgaaaaa ccaagatcaa ccatatcgaa
    21601 cagaaaaagt taagtactta cgtgggacaa atgaatgtca ttttatttgc accagaagat
    21661 ctttctttag taaaaggcag cccgcagacc aggcggaagt ttttagatat ggaattaggc
    21721 cagatcaatc caatctatct ttatgatctt gtccaatacc aagcaatttt gaaacaaaga
    21781 aaccagtact tgaagcaatt gaatgaaaaa aaacaaactg atttgcttta tttggatatt
    21841 ttgtccgaac aactagcagc gtttggcagc aaagtattga aagcaagagc acagttcgtc
    21901 aagcgattgg agttttgggc taattctttg catcagcaaa tcacccatca aaaagagcag
    21961 ctggaaatcg agtatctcac cgcagtcgat tctttagaaa cacatacgca agagcaaatc
    22021 caagaacaat ttctcgcact tttgaaccaa aataaaaaga aagacctttt tcggggaaca
    22081 acgacagttg gtcctcatcg tgacgatctt tcctttttta ttaatcagaa aaatgtgcag
    22141 acttatggat cgcaaggaca gcaaagaaca actgcactaa gtgtcaaatt agcagaaatc
    22201 gatttgatta aagaagagac aggggaatat ccaatccttt tgttagatga tgtgatgagc
    22261 gagctggatg ataatcgtca actccatctg ctagaaacaa tcgaaggaaa agttcaaacg
    22321 ttcttaacaa caacgacact tgaccatgta aaaaataaaa tgactgttga acctgagatt
    22381 ttttatgttc aacaagggaa actaaaagga gaagaaactg aatgacagaa gaaagaagtt
    22441 tagtagaacg cgctaaagag tatgatgcca gtcagattca agtacttgaa ggacttgaag
    22501 ccgttcgtaa acgacctggc atgtatatcg gttcgactag ttctgagggt ctgcatcacc
    22561 ttgtttggga aattgtcgat aattcgatcg atgaagtatt agcaggtttt gcaacgaaaa
    22621 tccatgtgat tattgaaaaa gacaacagta tcaccgttat tgacgacggt cgggggatac
    22681 ctgtagatat ccaggcaaaa actggacgtc ctgcagttga aacggtcttt actgttttac
    22741 atgctggagg aaaattcggc ggtggtgggt acaaagtttc tggtggactt cacggagtag
    22801 gatcttctgt tgttaacgcg ctttctacac agcttgatgt caaagtatac aaagaaggaa
    22861 atgtgtacta tcaagaatat cgtcgtggag ctgtagtgga cgacttaaag attatcgaac
    22921 aaacggatcg ccatggaaca acggttcact ttacgccaga ccctgaaatt tttacagaga
    22981 caactgagtt tgatttttca aaattggcta cccggatccg tgaacttgct ttcttgaacc
    23041 gcgggatgcg tatatcgatt gaagacaaac gagaagaaga accagtcatc aatgaatacc
    23101 attatgacgg cgggattaaa agttatgtcg aatacttgaa tgcaaacaaa accgtgattt
    23161 ttccagaacc tgtatatttg gaaggtgaac agcaagatat cgcagttgaa gtctctatgc
    23221 aatatacaga cggctaccat tccaatatca tgagttttgc caacaatatc catacgtatg
    23281 aaggcggaac acatgaatca ggattcaaga ctgctttaac tcgtgtgata aatgattatg
    23341 cccgaaaaca aaagctgatg aaagaaaatg acgacaactt gaccggtgaa gacgtgcgag
    23401 aaggattgac agctgttatt tctatcaagc atcctgaccc tcaattcgaa ggacaaacaa
    23461 aaacaaaatt gggcaattcc gaagtacgga cagtcactga tcggttattt agcgaacatt
    23521 tcatgaaatt cttgctggaa aacccaagtg tgggccgtca aattgttgaa aaaggattac
    23581 tagcttccag agctcgtcta gcagcgaaac gtgcgcgtga agtaacgaga cgtaaaggtg
    23641 ctttagagat tagtaatctg ccaggaaaat tagcggactg ctctagcaac gatcctgaaa
    23701 aatgcgaatt gttcatcgtc gaaggagatt cggccggcgg atcagctaag caaggtcgta
    23761 atcgagagtt tcaagccatc ttgcctattc gtgggaaaat cttgaatgtt gaaaaagcaa
    23821 gcatggacaa gatcttggca aatgaagaga tccggtcttt atttactgct atgggtacag
    23881 ggtttggaga agattttgat gtatcgaaag ctcgctacca caaattagtt atcatgactg
    23941 atgccgatgt cgacggcgcg catatccgta cattgctgct gactcttttt tatcgttaca
    24001 tgcgtcctat tgttgaagct ggatacgtct atattgctca gccaccttta tacggggtaa
    24061 aacaagggaa gaatattacg tatgtacaac caggaaaaaa tgcagaagaa gagttgaaac
    24121 aagtcgttgc ttcacttcct gcctcaccaa aaccaagcgt tcagcgttat aaaggacttg
    24181 gagaaatgga tgatcatcaa ctatgggaaa caactatgga cccaagcaat cggatgatgg
    24241 cccgcgtcag tgtggacgat gctattgcag cagatcagat atttgaaatg ttgatggggg
    24301 atcgagtaga accccgtcgt gcattcatcg aggaaaatgc acactatgtg aagaacttag
    24361 atatttaatc atgaaggagg aaaaagaatg agtgaagaaa tcagagaaaa catccatgat
    24421 gtcaatctaa ccagcgaaat gaaagactcc ttcatcgatt atgcgatgag tgttattgtc
    24481 gcacgtgcac ttcctgatgt cagagatggt ctgaaacccg ttcatcgccg tattttatac
    24541 ggcatgaacg aattaggtgt aacaccggat aaagcccata aaaaatctgc cagaatcgtc
    24601 ggagatgtca tgggtaaata ccatcctcat ggagactatg cgatctatga gtcaatggta
    24661 cggatggcac agccattcag ctaccgttat atgctagtgg atggtcatgg aaactttggt
    24721 tctgttgacg gtgacggtgc cgccgctatg cgttataccg aagcacggat gagcaaaatc
    24781 gctacagaaa tgctgcgtga tatcaataag aacactgttg atttccaaag caactatgat
    24841 gatacagaaa aagaaccagt tgtactacct gctcgcttcc cgaatctttt agtgaacgga
    24901 acaacgggga tcgccgtagg tatggccacg aatatcccac ctcataactt gtctgaggta
    24961 gtagcggcta tcgatttgct gatggataat cctgatgtga cgaccaatga attgatggaa
    25021 gtattacctg gaccagattt tccaacaggt ggtttggtta tgggaaaatc aggaatccga
    25081 agagcgtatg aaacaggtaa gggttctatt accgtacgtg caaaagttga gattactgag
    25141 atgccaaatg gtaaagaacg gatcatcgtg acagaattgc cttatatggt caataaagca
    25201 aaattgatcg aacggatttc tgaactgcac agagacaagc gtatcgaagg gatcacagat
    25261 ttgcgggacg aatcttcccg tgaaggtatg cggatcgtca ttgacatccg tcgtgacgcc
    25321 agcgcatctg ttatcttgaa taacttatat aaattgacat ccttgcaaaa ttcatttgga
    25381 ttcaatatgt tggcgatcga aaaaggtgtg ccgaaagttt tgagcttgaa acaaattctt
    25441 gaaaactata tcgaacatca gcgcgaagtc atcacaagac ggacagtctt tgaaaaagaa
    25501 aaagcagaag caagggcaca cattttagaa ggtttgcgta ttgctttgga tcatatcgat
    25561 gaaatcattg cgattatccg cgggtctaaa tcagatgacg aagcaaaagc gacgatgatc
    25621 gaacggttcg atttatccga tcgccagtct caagctattt tagatatgcg tttacgccga
    25681 ttgaccggtt tggaacggga aaaaatcgaa aatgaatacc aagaactctt aaaactgatt
    25741 gaagatttaa ctgacattct ggcaagacca gaacgtgtta cagaaatcat caagaatgaa
    25801 ttagcagact tgaatcaacg atttggtgat aaacgacgca cagaattact agtcggtgag
    25861 gtcttaagtc tggaagatga agatttaatc gaagaagaag aaatcgttat tacattgaca
    25921 aacaatggtt acatcaaacg tttggcaaat aatgaattca gagctcaacg aaggggtgga
    25981 cgtggtgtcc aaggaatggg tatccacaat gacgacttcg tcaaaaatct ggtttcttgt
    26041 tctacccatg atacattgtt attctttacg aataacggaa aagtgtatcg ggcaaaagga
    26101 tacgaaattc cagaatacgg gcgcacagcc aaaggaatac caattatcaa tttactaggt
    26161 atcgattcga gtgaaagcat ccaggcaatc attgcggtga cgggagaagc agaagaaggc
    26221 cactatctct tctttacgac gcgtcaaggt acagtaaaac gtacttctgt cactgctttt
    26281 gctaacatcc gaagtaatgg tctgatcgca attggtttga aagaagacga tgagctcgtc
    26341 aatgtgctac tgacagatgg tcaatctaat atcattattg gaacacataa cggctactct
    26401 gtcactttca aagaagaagc agtccgcgat atgggacgta cagcttccgg ggttagagga
    26461 atacgcctgc gtgaggaaga ttatgtcgtt ggggcagcct taatgaagga agatcaagaa
    26521 gtattagtga tcacagaaaa aggttacgga aaacggacaa aagtatcgga atatccagtg
    26581 aaaggccgcg gaggtaaagg gatcaaaacg gcgaacatca ctgaaaaaaa tggtccacta
    26641 gctggattga ccactgtttc tggagatgaa gacatcatgc tgattacaga taaaggtgtc
    26701 attatccgtt tcaacgtatc gactgtttct caaactggac gttcaacatt aggcgtacgt
    26761 ttgatgaaga tggaagctga tacgaaagtt gtcacgatgg cagcagtcga accagaagct
    26821 gcagaacaaa cagttgaaga acaaggatca gaagaatagc catcaagact aattgtgtaa
    26881 gctagcaaga atgaccgatt aaaaaatctg gttattcctg ctggcttttt tgattagttg
    26941 aatttctctt aaaatattcg aaactgttca ttctgtattg cttttgacca aaatagagtg
    27001 tataatcaga gcttgtgagt agtctaatta attagataac tctccttgct cttaggaacc
    27061 taagagccga aagtccacag ggaggtgaat atcaatggaa aacacgaaat atgaaatcat
    27121 gtacattatt cgtccaaaca ttgatgaaga agcaaaaact gctttaatcg aacgtttcga
    27181 tgcaatcttg aaagataacg gagcagaagt tatcgaatca aaagattggg aaaaacgccg
    27241 tttcgcttat gaaatgaacg gttaccgtga aggtatctat cacatcgtta aagtatcttc
    27301 tccatcatca gcaaatgctg tcaatgaatt tgaccgtctt gctaaaatca acgacgatat
    27361 cattcgtcac atgattgtta aagaagaagc ttaattgttg tttcatgtga aacaatagaa
    27421 aacgatgaga ggagatgact attttgatta ataatgttgt attagttgga cgtctgacga
    27481 aagatccaga tttgcgttac acagcaagtg gtacggcagt cgcaacattt actttggctg
    27541 tgaatcgtaa ttttacaaac caaagcggca atcgcgaggc tgattttatc aactgtgtga
    27601 tttggcgcaa atcagcagaa actttagcaa actatgctcg taaaggaaca cttttaggtg
    27661 ttactggacg tatccaaact cgttcttatg ataatcagca aggacaacgt gtatacgtga
    27721 cagaagtagt agctgacaac ttccaattgc tggaaagtcg ttctgcatct gaaaatcgcc
    27781 aacaaagcgg cggattccaa agttctggtc aatcagctgg cggatttggc ggaaacaata
    27841 actctaatca aacatctcaa tcatccaacg gtatgcctga tttcgaccgt gatacttctg
    27901 acccattcgg ctcaagttca acgatcgaca tttcagacga tgatttacca ttctaaacga
    27961 atttgatagg agggaaatag aatggcacaa caaagaagag gcggacgtaa acgtcgtaaa
    28021 gtagactaca ttgctgctaa ccatattgaa tatatcgatt ataaagatat cgaattacta
    28081 aaaagattta tttcagaacg cggtaaaatc ttgccacgtc gtgtaactgg tacaggcgct
    28141 aaaaaccaac gtaaaattac tgttgcaatc aaacgcgctc gtatcatggg cttgctacct
    28201 ttcgttagcg aagactaata aatcctagga attgaatgac tgatcctttt gatcagctat
    28261 tcaattcctt tttttctgta aaaataacct ctattcgttt agtcgctcga atgacgaaag
    28321 ttatggtaaa atcaaattag tcatatatgg gaggaaaatt atggaaaata aaaacaatca
    28381 tttatccccg aaacctctct tccttatcct gtttatatta atcgaagtat tggtagtctt
    28441 aattttacca tttcgttttg ttagcgcttc ggtcattcta ttgatgaatt tatggggtgt
    28501 tcgctattgg ttgaaaatga aacgttggct tattttaaat aaacgtgaaa aaatccagca
    28561 ggcttcgact gtagctgaaa ccaatctagc ttatgtctca gaagtcatgc ctgtaggagt
    28621 gattgcttat cagccatcaa gccaaaaaat cgaatggctc aatccattag cattgaaaat
    28681 caaagatcaa agtggattaa cagaggaaga attgctagat acttttttta ctgcaaaaga
    28741 aaaaaagaaa aaccaagtct atctagcaga taaaacattt cagtttgata tggatacagt
    28801 aaagggtgtt gtttatttta atgatattac ccaaaaccag ttgttaaaac tccatcagac
    28861 gaacagtcag ccggtgattg gtattttatc tattgataac tatgcagatg ccattgataa
    28921 attggatgac aaagagattt cttatttgaa ctctttcgtc actacactag tttctgactg
    28981 gatgaatgaa taccatgtct ttttcaaaag aatcaacgca gaacggtttt tcttcgtagc
    29041 acagatgatc gatctgaaaa agatgatgga taaaaaattt gatttgctgg atcgctttcg
    29101 agcagaagct gaaaaacaga gctcgcctat cacccttagt atggggattg cttatggctc
    29161 aagaaccctc gatgaaatcg gtggagaagc tcagaataat ttagatgttg ctttagtccg
    29221 tggaggcgac caagttgtcg tgaaagaggt aaaagaagga gcaaaacctg tttattatgg
    29281 ggggaaatct gctaagaatg tcaaacgcac gcgtgttcgt tctcgagcga tgagtaccgc
    29341 tttgcgtcgg atttttgatg aaacaggcga tgtgtttatc atggggcaca agtttccgga
    29401 tatggatgct ttaggctctg cttttggtgt cagttgtctg gcacgcttca accagaaaaa
    29461 agcttacatc gttatcaacg agaacgagat cattccagat gtagaacggt gtcttgaaga
    29521 aatccataag catcctgaat tagaaatgca actaatctct ccagaacgtg ccatagagct
    29581 gaaaaaagaa gaggacctcc tcgtgatggt ggattaccat aaaccttctc tatcgatctc
    29641 tcagggccta tatgagcaat ttgataagat tgtgatcatt gatcaccatc gtcgtgggga
    29701 agagtttcca agtaaacctc tgctgactta tatcgaatct tcagcttctt cagcttcaga
    29761 gctagtttct gagctgatcc aataccaaag cagcaaaaca aatcgcttag atcgttttga
    29821 ggccacatta ttgcttgcag ggatggttgt agatacaaat agtttcaatg tccgtacaac
    29881 tgcacgaact tttgacgttg ccagctattt aaggtcttgt ggagcagacg catccttagt
    29941 acaatattta ttgagctcgg atttagcgac atacttggag atcagcaatt tgattgctgg
    30001 aagcgactat gtgacttctg atattgttgt ggcggcaggg acagacgaaa aagagtataa
    30061 tagtgttata gcagcaaaaa cagcagatac attgctttcg atgatcaata tcaatgcagc
    30121 tttcgtcatt acaaagcgga cagacggctt gattgggatc agtgccagaa gtaccggcac
    30181 gatcaacgtc caaatcatca tggaatcctt aggtggcgga ggacacttca caaacgcagc
    30241 tactcaactg gaaaatacga cagttgagga aacaaaacag aagctatttg ctgcaatcaa
    30301 agaaaatatg aatcaaatat atgataagga gtaatccaaa tgaaagtaat ttttttacaa
    30361 gatgtaaaag gaaaaggtaa aaaaggggac atcaaagaag ttccagcagg ttatgcacaa
    30421 aattatctaa tcaaaaataa cttggcaaaa gaagcaacga aaagcagtat cagtcagtta
    30481 gcaggacaaa agaaagcaca agaaaaacac gaagctgaaa tcttggaaga agctaaaaag
    30541 ttaaaagaat tcttggaaaa agaagatact gtagtcgaat tgaaggcgaa agcaggggaa
    30601 gacagccgtt tgtttggttc gatcccttca aaacaaattg ctgaagggtt aaacaaacaa
    30661 tacaaaatca aattagataa acgaaaaatc gaattagaaa atccgatccg ttcactaggc
    30721 tacacaaacg tacctgttaa gcttcatcat gaggtgacgg ctacgatcaa agttcatgtc
    30781 gtacaagaat aacaaatctt gatagcgatg ttcagctggt aagtataacc agaaggaaaa
    30841 aagacaaacc gaaatttatc tgagaaaaca gctgcagcaa tgaaagcaaa agctgcaaat
    30901 cgatttcaga ttgatttcgg tttctttttt gggaacagca aaatagcata aagaaaacga
    30961 gtataataaa agagaactat ttttttatta cactttcggt acgaatgatg acttttctct
    31021 cattttcttg taaaatgaag caattgaaga caaggagcga tacgtatgaa tgaagtgtgg
    31081 caggatcgaa tcccaccgca aaatattgaa gctgaacaag cggttttagg ctctgttttt
    31141 cttgacgcag aaacgatcat tgatgccatg gagtacatag agccaaaaga cttttatcgt
    31201 cgtggccatc aaattatttt tgagacaatg atcgagttaa atgatcgtaa tgaagcgatt
    31261 gacgtggtta ctgtcaaaga ccggctggaa caagccaatt tgttagaaga tgccggtggt
    31321 ttaagttatc tctcagactt ggcattagca gttccgacag cagcaaatat cgtctactat
    31381 gcgaaaatcg ttgaagaaaa atcattgctg cgtacattga tccagacagc aacaggaatc
    31441 gttacgaaag gattcgagca aggagaagaa gttcaaacaa ttttagatga tgccgaacgc
    31501 agtatcttgg aggtttcgga aaaaagaaat cgaagcggct ttttatctat tgctgaagta
    31561 ctgaactcca cgattgaaaa tatcgatcga ctagctcaaa acaatgaaga aatcacaggt
    31621 ctaccaactg gctatcaagg tctggataaa atgacagcag gtttccagcc cgaagaacta
    31681 atcattcttg ctgcacgtcc agcggtaggt aaaacagcgt ttgcattgaa tattgctcaa
    31741 aatgtaggaa caaaaacaga tcgttctgtt gcgatattta gtttggaaat gggggcagaa
    31801 tctcttgtaa atcggatgct ttgtgcagaa ggttcgattg aggccagcca tttacgtaca
    31861 ggtcagcttt cggaagaaga atggtcgaat ctgatcgttg cgatggggag tctttctcga
    31921 gcaaatattt ttatcgatga tacaccaggg atcaaaatct ctgaaatccg tgcgaaatgt
    31981 cggaaactag cgcaagaaaa aggcaatcta ggcttgatat tgatcgatta tttgcagttg
    32041 attgaaggta acggtaaaga gaaccgtcaa caagaagtat cagatatctc tcgccaactg
    32101 aaaaaactgg cgaaagagct gaaagtacct gtcattgctc tttcacagtt atctcgtgga
    32161 gtagaacagc gacaagacaa acgaccagtg ctaagtgata ttcgtgaatc cggatcaatt
    32221 gaacaagatg cggacatcgt agccttcttg tatcgagatg attattatga aagagaaggc
    32281 ggagatgacg atgatggaaa cgagccaaaa aatgacaacg tcatcgaagt catcatcgag
    32341 aagaaccgaa gcggtgcacg aggaacagtt gaactactgt ttatcaaaga atacaataag
    32401 ttttcgtcgc tttcgccaag agaagaattt taaagaagag gttgtgagcc ttgagcaatc
    32461 ccgagagctc tcgtaagaag atgcaaggag taaccgcagg aacgactaga gtacttaatt
    32521 taaacagatc tccaaaggga ttgtgacaaa agtcttgtca cagtcccttt ttccgaataa
    32581 acggtgttca atcagctgac ccttggtatt agctcagcaa acgtcaaaat atgaggaact
    32641 attttgacgt ttgtttctct aatactcagg tcaaaacgct gatcttacga cctcttttcc
    32701 ttaaacggtg ttctcctgcc aactcctcgg tcacaagtca caatatcatc aaacatgaga
    32761 agcagttttt tgaaattgct ccttgtgact cagagttaaa cggcaggctc acaacctctt
    32821 tcttaaacgg tgttcaaaag cttgcatctg cggtaatacg cccaatcaaa cgaaaaatat
    32881 gagaagctat ttttgtttgg ctgttcttat tacttgatgc aggacagctt tttcacaacc
    32941 tctgattcac ggtgttccaa aagtagcttc ttcgaaaaat aagtcgaaat ttcacaaaaa
    33001 tttgagaagc aattttcgta cattccttct tatttcttga agctgaccac ttctgttaca
    33061 acctcttttt taataaccat tctaaaaaga taaagttcgt tttttaagcg tttttataaa
    33121 ataaaaataa aaatcattcg attttatttg aaatatgtat ctattattga tacaatggtt
    33181 caggattaaa taatatagaa cgaggtgttc acatgtcatc tgtagtagta gtaggtacgc
    33241 aatggggcga tgaagggaaa ggaaaaatca cagactttct cagtgaaaat gctgaagtga
    33301 ttgcacggta ccaaggtggc gacaatgcag ggcacacaat taagtttaac ggtgtgactt
    33361 ataaactgca tttgattcct tctgggattt tttataaaga caagatcagt gtgatcggaa
    33421 acggcgttgt agtcaatcct aaatcattgg ttaaagaatt agcttattta catgaaaaag
    33481 gtgtagaaac aagtaatttg cgtatttctg accgcgcgca cgtaatcttg ccttatcata
    33541 tcaaattgga ccaattacaa gaagatgcta agggcgaaaa taagattggt acaacgatta
    33601 aaggaatcgg acctgcctat atggataaag cagctcgtgt aggtatccgt attgcagact
    33661 tattagataa agaaattttt gaagaacgtc ttcgtttgaa tctagatgac aaaaaccgtc
    33721 aatttgtaaa aatgtttgat tcagaaccac ttgattttga cgatattttt gaagaatatt
    33781 atgaatacgg acaacaaatc aaacaatacg tcacagatac ttccgttatc ttgaatgatg
    33841 ctttggatgc aggcaaacgc gtgttgtttg aaggcgcaca aggtgtaatg ctggatatcg
    33901 accagggaac ttatcctttt gttacttctt ctaacccagt agccggtggg gtaacaatcg
    33961 gaagcggtgt agggccttca aaaatcaata aagttgtcgg cgtctgcaaa gcctatacgt
    34021 cacgtgtcgg tgatggacca tttccaactg aattatttga tgaaacagga gaacaaatcc
    34081 gcgaagtagg acgagaatac ggtacgacaa ctggtcgtcc gcgtcgtgta ggctggtttg
    34141 attctgtagt tatgcgccat tctaaacgag tttcaggtat taccaaccta tctttaaact
    34201 cgatcgatgt attaagtgga ttggaaacag taaaaatttg tacggcttat gaattagatg
    34261 gcgaactaat ctaccactat ccagcaagct tgaaagagtt gaaccgctgc aaaccaatct
    34321 atgaagaatt gccaggctgg tcagaagaca ttacaggatg caaaacactt gcagaactgc
    34381 cagaaaacgc acgtaactat gtacgtcgta tctcagagct tgttggtgta cgtatctcaa
    34441 cattttctgt cggacctgat agaacacaaa caaacatctt agaaagcgtc tgggcgcaaa
    34501 tctaaggata aaaacgacga ctgaatcagg acgttcttac cagagcgagt cgaagtgttt
    34561 atctatacga taataataac ttgaacactg tttattcaga ataagggact gtgacaaaaa
    34621 ccgtgtcaca gtcccttttt ccgaacaaac ggtgttcaaa agcttgcatc tacggcacta
    34681 agcccaatca gatgaaaaat atggagtgct atttttatct gattgttctt actgcttgat
    34741 gcagagcagc tttttcacaa cctctccact aaacggtgtt ctcctgccaa ctcctcggtc
    34801 acaagtcaca atattcaaca aacatgagaa gctgtttttt gaaattgttc cttgtgactc
    34861 ggagctaaac ggcaggctca caacctcttg gtaaacggtg ttcaatcagc tgaccctcgg
    34921 tattagctca aaaaacggca aaatatgagg aactattttt tccgtttttt tctctaatac
    34981 tcaggtcaaa acgctgattt cacaacctct gattcacggt gttccagaag tagcttctac
    35041 ggaaataagc cgaaatttca caaaaatttg agaagcaatt ttcgtaaatt ccttcttatt
    35101 tcttgaagct gaccacttct gtcacaacct cttattttga ttagaacttg cgaaaataaa
    35161 ccggttaaag tgtgtctagc gcaagagatt tgctataata gacgataagg gtaaaatgta
    35221 acgagaaggg gttgaaaaat gtgtatcaaa ttgtaacaga ttcttgttgt gatctgccat
    35281 accatgtatt agcaaaagaa aaagttgatt ttttatcaat gaatctccaa ttgaatgatc
    35341 aagagttagt agatgacctc ggaaagacat tcaattatga ccgctacatg aatcagctga
    35401 aaaatggtgc gatgcctacg acatcacaaa tcaatgttgg ccgatattta gaatttttcc
    35461 gttcttatgc ggaaaaacag attcctgttt tatatgtggc tttttccagt ggattaagcg
    35521 gctcttacag tagtgcgctt caagcggttg aattgctgaa ggaagaatat gaagaccctg
    35581 agatctatgt ttttgataca aaagctgcaa gtattgggga agggctttta gtattggaag
    35641 ctgtaagatt gaaaaaagaa ggtaaaagca tcgaagaaac cctagagtgg ctggaaagta
    35701 attatttgaa agtccattca tgggtaacag tagacgatct gaaatatcta gaacgaggcg
    35761 gtaggatttc caaagcaagt gctactcttg gcagtctatt gagtgtaaag cctattatta
    35821 cggtggataa agaaggaagg ctgacgaatg ttgacaaagt tcgaggaaga aacaagtcga
    35881 tccagacaat cgtcaatgag acggtaaaaa caatcgttgc accacttgat caaatcgttt
    35941 atgtggctta cgctggggat ctagagagcg cggagaaggc aaaacggctt ttggaagagc
    36001 agataaagat gaaagatgtg aagctttatc ccttaggacc aacgatcgcc agtcacacag
    36061 gctatggctg tatcgcgatt ttttcgatgg gaatcagtcg ataaaagcag taactcattc
    36121 tgtaaaaaag cccagaaatt gattgtaaca aaaacagcga ctcttaaaag atagatgagc
    36181 atcctatctc ttagaggtcg ctgttttttt attttttaag ctaaataggc cattatgccc
    36241 ataactcgtt caattgtttt tgaatatctg cattttccaa aaattcatca tatgtcgtat
    36301 cagctcgatc gatcacacct ttatccgaaa cggcaatgat gcggtttgcc aatgtttgga
    36361 taaattgatg gtcatgtgac gcaaataaga ttgagccagt aaatgccatc aagccgtcat
    36421 tcaatgctgt gattgattct aagtctaaat ggttcgttgg atcatctaat acaagtacgt
    36481 tagctttcga aagcatcatt tttgaaagca tgcaacggac tttctctcca ccagataaaa
    36541 cattcactgg tttcaatact tcttcaccag aaaacaacat tcttcctaag aagcttctta
    36601 agaaggtgtt atcatcttct tctttacttg catattgacg caaccagtct aaaatcgtta
    36661 gatcagtatc gaattctttc gttgtatcct ttggtaaata agaattactt gttgtcacac
    36721 cccaacgtac tgttccagta tctggcgtaa tttcacccat gataactttg aatagcgttg
    36781 ttgttgttat atcattttct gcgacgaaag cgaccttgtc ttctttgctc aaattaaaag
    36841 atatatcatc taaaattttc tttccatcaa tcgttacact tacattttct acctgcaata
    36901 agtcgtttcc aatctcacgt tcaggtttga atccaacgaa tggataacgt ctagaagaag
    36961 gttggatatc gtctaaagta attttttcaa gcattttttt acgtgaagtt gcttgttttg
    37021 attttgatgc attcgcacta aagcgggcaa taaattcttg gagttctttg atttgttctt
    37081 cttttttggc atttgcgttt gcttgaagtt tagccgctag ttgactagat tccaaccaaa
    37141 aatcataatt tcctacataa agtttgattt taccaaaatc tagatccgcc atatgtgtac
    37201 atactttgtt caaaaagtga cggtcatggg atacaacgat cacagtattt tcaaaattga
    37261 tcaaaaattc ttccagccaa ttgatcgatt ggatatccag accgtttgtt ggttcgtcta
    37321 gcaaaagaac atcaggttta ccaaatagtg tttgtgctaa taacactttt accttttgtc
    37381 caccagttaa ttcactcatt tgctgatggt gcaattcttc agggatgttc agaccttgca
    37441 gtaaaacagc tgcttctggt tcagcttccc atccattaag ctcagcaaat tctccttcta
    37501 attcagctgc tcgaatacca tcttcgtcag tgaaatcctc tttcatgtaa atcgcatctt
    37561 tttctttcag cacttcatac agacgcttgt gtcccatgat gactgtttca agtacagtat
    37621 agtcttcata gtcgtaatgg ttctgtttca atacggccat ccgttcatct ggtcccatag
    37681 taacggaacc tgtggaaggc tgcagatccc ctgataacac tttaaggaac gttgatttac
    37741 ctgcaccatt tgcaccaatc aagccatagc agtttccggg ggtaaattta atattcacat
    37801 catcaaatag tttgcggtct gaaaagtgca aactcacatc atttacagta atcaattttt
    37861 ttcctcactt ttatcattat tagttttgac aagtcttata gaattatata ggggaaaggt
    37921 aaaagattca aggcaatcga acattctttg tgtttcacgt gaaacagaat gaaaaaaata
    37981 aaaataaaag cggagcgaaa atagctacta taggatttat tagtttgcaa cagcataatt
    38041 tttgacgtta tttataagag ggagtactat aaaactgtaa aagaaatgaa agaaggattg
    38101 aaaatggatg aaaaaagaac catcgactgg ggctcattga ttttgggaat tttatttgta
    38161 cttgtctcat tgttatcatt tcaagaccct gtcggaaatt tagttgcaat tgtagtagtc
    38221 tttgcgattt ttgcctttat taaagggata tttgaactgt ttgtacggaa cagattaaaa
    38281 gagctaacag gatataaagg aaagatgccc ttaataatcg ggatcatcga tattctagtt
    38341 ggcgtctttt tcttattcaa tattggtgca ggagtcgcag cattgccttt tgtttttgca
    38401 gtatggttta ttgctgattc agttttagcg ttatttactg ctgacttggc aagaggagta
    38461 agtgaaggat attattggtt tacgatcatc gtcaatattt taggaatttt attaggaatt
    38521 ttcttattgt ttaatccaat ttcttctgcg ttgacattaa gctttttagt tggattctat
    38581 tttatgttat ttggtatcac acatatcgtc tatgcattta gataagtaaa atcaaacaaa
    38641 taaaaaagcc ggcttccgat tacagtttta ctgttgtaat tggaagccgg cttttttatc
    38701 atggacaaat atgcacgagg tgatttttag gtgtcgagac aaatttatgc aaaaatgtga
    38761 gaagctatat acacaaattt tatcttaata ctcgaagcat agcgcagttg tcacaacctc
    38821 ttttcatata attcattcaa tactggcaaa tcatggactt catatttttc tttgaatgct
    38881 tggatttctt cttcagaata ttttcttgga tctaaatgca gatcttctac tggcaatggg
    38941 cgcttgttat tgatcttcac atcgattact acaggacgat ctgattttgc agctgcatca
    39001 aaggcaaatc gaagttgcga gtattctgtg actgtaaagc tatttgcccc gagcgcttct
    39061 ccaactttac cgaaatctgc atcttctaaa aatacaccaa atttcttttg ttccgtatct
    39121 tcttgttctg cttcgatgaa accaaaagaa tcattcgaaa agacaacatt aataattgga
    39181 agctgatatt tgacttgtgt aatgatatct tgcatgacca tagcaaaccc gccatcacca
    39241 cttaaggtaa atacttgttt ttctgggtag cttagttgtg cggcaattcc tcctggcaca
    39301 ccattaccca tagttgcgaa ccatccagat gtcgtatgtt tttgtttacc gttcatattc
    39361 aataaacgaa tagaatggat agtcgtgtta ccgacatctg tgacgaaaat cgcatcgtct
    39421 tcagcaatac gattgatttc tttatagaca gcctctggac gaatcggttc ttcctctcga
    39481 tcttcaaaag atttcaacca atttacccat ttttctttat tttcttgatt tgctaggtac
    39541 cagctatcag cagggcgagc atcccctaac tcgacaagtc tttgcaaagc tgtgtttgca
    39601 tcaccgagga tcgacaaaga tgtttcatgg cgtcgtccaa attttgacgc atcgatatcg
    39661 atttggataa attcagcagt tggattaaaa aatgcacgtc caaatgggaa gtcgctaccg
    39721 acaaataaga tcagatcggc ttctgccaat gcttcatttc cgggtttagt agcgacacgt
    39781 ccagcaaaac caagaaagtt ttcataatca tcaggaataa ttccttttgc tagaacggaa
    39841 gcgacaacag gaactgaaaa atgttctgag aattctttga tttgcggaaa tccgtttcgc
    39901 gttccttgac caacataaag aactggtttt tttgcttttt caagataagg caaagcagct
    39961 agcaaatctt tttcttctgg taatatcaca cttgttttgt gagtgtgagc ggtggagaaa
    40021 ggttgttcgt caatttcttc aaaaccaaga tcaacaggga tagtcacgat agccacgcct
    40081 ttatgctcat aggcggcttt aatggcttca tctactacat gcggaagact ttgtggtgtc
    40141 atgactgtac gattgtatac gctaacatct gcgaaaatag gattttcatt tagttcctgg
    40201 aaagagttat agttcatgga tgtagaagca acttgtccta agaacgctac tagaggcaca
    40261 tggtccatct gtgcatcata caaaccattg atcaaatgtg tcgcaccagg tcctgcagag
    40321 ccgaagacag cgccgacttt ccccgttaat tttgcatctg cagcggctgc gagtgcgccg
    40381 acttcttcat gacgaacttg aatgtagttg atttcggttt tttcttgata tagcgcatcc
    40441 ataattgaat tgaaagatcc tccgggaatg ccgtaaatat ggtcgatacc ccagctttca
    40501 agaactttta ccattgcgat tcctgcattg atttttgaca tggtgtttca ctcctttagc
    40561 aatgatgata cataaatttt ttgtatctag agtatagaac tttttatcga aaatcccaac
    40621 taattcgtct tttcacaaaa atgtagtcat gtttttggga atatgctaga gtagtacata
    40681 gatagattca agagagggga atggcatggt tgaatggcta ttgagacttg aaccttggca
    40741 gcaagcactt gctgggacag cttttactta ttttatgaca gctcttggtg ccgggttggt
    40801 ttttttcttt aaagaaatca aaaaagaagt gctaaacttg atgttaggat ttgcttctgg
    40861 tgtcatgatt gctgcaagtt tttggtcttt gttagatccg gctattacaa aagcagaaga
    40921 aaatggagat attgcttggc tagttgtaag tattgggttt ggtttaggcg gtgtgttttt
    40981 atatatggca gataaaacac tgccccatat gcattttgga ccacaacatg aagcagaagg
    41041 attgcctacc catttaaaac ggacgatctt gcttgttttt tctattacat tgcataacat
    41101 tcctgaaggt ttagccgtgg gggtagcatt cggtgcagca gcaactgcgg ataatccgac
    41161 agccgctata ttagccgctg tttcggtggc gctaggtatt ggtatccaaa acttcccaga
    41221 aggagcagct gtatcaatcc cgttgcgaca agaaggtctg agtcgtaaaa aggcctttgt
    41281 ttacggacaa gcttctggaa tcgttgaacc aattgctgga gtgattggag cgctactagt
    41341 aacgaaagtg gagctgctgc ttccttatgc acttgctttt gccgcaggag caatgattta
    41401 tgtcgtggtg gaagaattga tccctgaagc acagcagaca ttgtccagca aacgtcattt
    41461 tgcagttttt ggcgtcatga gtggatttat cattatgatg attttggatg tggcattagg
    41521 ataaataaaa aaaatatata tgatatatca aatagggccg cgaacttata tcgcgaccct
    41581 atttttttag taagttatat cataatgaag tgaatggtat catacattaa taatagtttt
    41641 ttctatgtgt atttcctttg atccagcgaa caagccagat gataagtgcg attgggatta
    41701 gaactttgac cgcaaaccat aaaagcccgc ttaggacact gacaaaaaga ctgatgacga
    41761 agctagctgc aattagcact aaaagaacca tgaagatatt taagaacata tttttttgac
    41821 gcatacaaaa ccctcctctt gttcattctt tactatagaa aaagtataac agggattgtt
    41881 taaaaacacc tccaactgaa gactgatttt tacttcatct acttaaatga tcaaacaatt
    41941 gctttttctc tggtttatca tctaaaatag agcggaaaag gaggagaata tcatgcctaa
    42001 aaatcgtctt gaagcattta cagatgctgt aatcgcgatt atcatgacta ttttagtttt
    42061 agaacttcat gaacctaacg gagacacgtg ggaggcgctc gctgcattag gttaccgatt
    42121 ttttatctat attgtcagtt ttatcatttt agcgatttat tggaacaatc atcatcattt
    42181 atttcaagtt gtacataaga taaatggacg ggtcttatgg gcgaacagtt tctttgtttt
    42241 tgcgctttcg ctttttccgt ttgcaacttc atggatcagt gagtacatca atagcctggc
    42301 tccagaaatg acttatggtt ttgttacctt gaccgctaat attgcgtatt atatcttggc
    42361 aaaagaactt gtcaaagcaa ataaaggcca cgagaaagca ttgaaggcga tgttgaataa
    42421 ttacaagaaa tcgtatctat cgattggttt gaatgtgtta ggaattgtct tagggtattt
    42481 agtagcgcca gtagccgtgc tgatcgcaaa catcgtgatt ctagctgctt ggatcatccc
    42541 aagtcgtacc atcgaatcac aatatcggta aaatgatccg tgtttaaaat ttaagagaaa
    42601 aatatagaaa aaataaataa acgatcaaat atccgagtga tactagtatt caaagaggga
    42661 tgcagatgga tcaaaaaaga attcatgtag gactgattac tagttttgtc acgatgctta
    42721 tttatttgga tcaaggtcat ttgacttgga cagtagttgg tgtcgtagga tttttgacat
    42781 tattcacagc aataaaaaaa taaacatctt tatgtaatct gatgttctat ctatttaaga
    42841 aaagcatgtt tacagcagta tgattctgtt gtaaacgtgt ttttatttgt ggaaaattta
    42901 atttttagaa aagtgtatcg aaaaatagat agttagtttt tccacagtgg ataacttcgt
    42961 aagagataaa aataaaaaat tagtcaaaga agggcgtata gatttctttt gataggagat
    43021 atacaacaga aatgaataaa tattaacgtg ttttcaaaca gtataaaacg gtgtggtttt
    43081 tggaataaaa aaataacata tctcatttta cgcttttgtt attttatact taaaaaataa
    43141 cgacacagaa aataccgtgt ctcagatttc tctactactt ctaaaaacta ctgttttttt
    43201 cctttgaaat gccttcaaaa agtaggatca ggcagaatat actggcagaa tcaaggagaa
    43261 gggagcctaa ataaggagaa aaaccataga aaatataaag taattactta taaaacaagc
    43321 tttttataga aaaataatat acctgagtag ggtataaatt aagacggaat tcctttctag
    43381 attgagatat tgactaggaa gaatgttaag attttaagcc tttaagctaa aaaaaaaccc
    43441 tttttgttaa ataaggggaa tatagcctgt cagtttcaaa ctgcaagtcg tcctgctgca
    43501 ataatatagt agattagaga tgaaaataga tttttttaaa ccaatgactg aataaaaaga
    43561 ccgtcataga tacacattgg aatgtgtcta ttacggtctt tttttacgaa cgattttatt
    43621 gaagacggac agttcgggtc aaaaataaaa aagctacgat cagaaataag aataaaatgt
    43681 atcccaaaat aatggctaga cggtctaact tatttgtcga tagatgtttg tttagaagtt
    43741 gataggccca taaacctatc agtcctcctg ttgtattagt tatcaaatca gtgatgtccg
    43801 ctacgccaat accgaatagg aattgaatca attctgccaa gaagcttaca aggaaaataa
    43861 tccctaattt tttagaaaaa gaaagtctct tgaaattgac attcaaaagc accccaaaag
    43921 gaaaaaagac aatcaagtta taagtggtct cacgtaatac cgtcgtattt tcaaaagaaa
    43981 aagtagaaaa agggacgaga ttgacattgc tgtaagaata agtgagaaat tgtggaagat
    44041 taaatgatag cttgaataaa atcaaccata atagaaagaa taaataaatc cctaataaaa
    44101 attttgatac ataccgcata gacatctcct ccatttaaaa agtataccat cgaaaaccgc
    44161 tgaaaaatca taaaaaagta tgaagtttaa ggaccaacgt agttatcgta cgaaaattag
    44221 aagctttttt ttgttgggaa tccggtttct ttaacaaagc taagatagcg agttgcttgg
    44281 aaaagttgct atcttttttc tgtcaaaacg actcggaatg ttcgtttttt gttctaattt
    44341 ctccaagttg tttacttttt gatactaagt ttatgaaaaa tcaagaaata gttgttatac
    44401 taggtaatgc tgacaaacgg gattggcatt tcatcttggt tcagcttttt ttccaattac
    44461 taggaaggag ggatagattg ttcaagactt attttcgttt caataaaata caagataatc
    44521 cttttcgtat catgaatctg atgctactta tctttattgt attatggtta ggttacataa
    44581 atcatgattt tttgatttca agtttttcat cattagggat ctttactttt ttctattatc
    44641 aaaacatacc gatgaaatat ttgctgaaac gtatgtttct gatcggttgc ggactgttgg
    44701 ttgctttcgt tttggggatc ttatcgactt atgttttatg gcttgaacca tttgccgtag
    44761 ctttggttgc tttttccagc agattcgtgt tgcgattatt ccatatttct aaaccaggtg
    44821 gcttgttttt tgcgatgctt tctgccatgg gaacgagcat gcagctgcca atcgctcaat
    44881 taccgatcgt ctcactttat ttttttatgg gtgtcgtttt tgccctgatt gcggcagtga
    44941 ttacaaaatt actggattca cggcctgagc agacaattga aaaagcaact ttaaaagaaa
    45001 gatttcacga agagccttta gtgatcattg attctgtttt ctacagcgct gccttatttc
    45061 tttccgttta tgtcagtcac ggcttgaatc tgcataatcc ttattggttg accttgtcat
    45121 gtgcatcgat tttgctagca gaaaacttgg atgcgatgaa gcatcgacaa gtccaatatc
    45181 tgattggatc gatgggtggg ttatgtgtgt cggcattctt gtcatttgtg ccgtttacgc
    45241 agttgcagac catttttctg atcacttttc tatatggaat cgcacaattt ctagttgcta
    45301 gaaattatgc agttgctaat atttttctta atccgatggc gttgatgcta tctacactta
    45361 ttcgtggagc atacttgatt tctttgatcg aatatcgttt tttagggatc gtcataggaa
    45421 gtttcatcgg tttaggtgtt gcgtgggtaa tgacggttgg tttgcaacat tatttaacgg
    45481 ttgttcgtca aaaattagaa tagaaggaca aatatatgtg ccaaaagagg ttatgacaag
    45541 atttttgtca caacctctaa ttcaaggtgt tcaaaagcca gattctcagc attaagtcaa
    45601 attcacacaa aaatgtggaa aggaactttc aaaaaaattt tatatttatt ctatcaaaaa
    45661 attttatatt tattctatca aaaaattttg tttatgatgg taactgaata aactttcctg
    45721 agatggtcaa tgctttcctt gaaagtctta cagatattct ttcttcatag aaagtttaag
    45781 ttttatagtt agtgtacaat aaaagtatgt ttataccata tggtgtccgg ataatcagtc
    45841 atttaaacaa tgaggttcaa agatatgaaa aggggaaatt tgatgaagaa aataagcaat
    45901 gaaatccaaa ttctggcaga aactttaact cttcccaaag tcgttataga gcaaatgatt
    45961 gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat atagtcatga actaatggat
    46021 cctaataaag caaaggcgac tgcaaagaaa atagacaaag tctatgaaga ggaacctttt
    46081 gcagcattag ctatttatct atatgcagct tctcaaagtt ggaaaaaaat ttatcaacca
    46141 ttgaagattc caagaactat ctatttggca acaatgaatg cttttactag atttattcat
    46201 gaacatttcc aagtaacagg aagctatcgc tttgaccgtg gtttttggat ttggcattac
    46261 actagtggct tgatctttcg gattggtgaa ttggagtacg aaatgacata ttttccgaaa
    46321 gggtacacgg atctaagatt agaaggaaag cagtgccttt ctcttcatat tccttccgat
    46381 gcagatttat ctcagaataa gataagagat agttacgaaa gtgccattcg tttttttgaa
    46441 ctttattttc ctgattacca ttaccaagtg atgtacacag atacttggtt actatcaccc
    46501 aatcttacta aatggttgaa aaaagagtca aaaatctgtc tttttgcagc cgattatcga
    46561 ttattatcag ttgatgaaca ggacgatagt ggagtccctt ggatctttgg acgagtagat
    46621 gcccaaatac atgattatcc agaaagtacg agtttacagc gacaagcaaa agagcaattg
    46681 cttgcaggcg agcacattgg ctctggctta ggtattctta cttcataagt ttagtacttc
    46741 gaaatagttt aaaagagcgt gagacaaaag taaagaacac ttttgtctca cgctctaaac
    46801 acgtataaac ggcggaagca gaagcaactc cttcggaaat aagctgaaat tcacaaaaat
    46861 ttgaaaagca attttcgtga atttcttctt atttcttgga gttaaacgct tctgtcccag
    46921 cctctttttt agtgttctat cgaacacaca gtacgttcaa taggtaaact agctataaca
    46981 atagatatta aagttaacgg acaagagggc cttttttagg tagctaaaac gtaataatga
    47041 aaatttattt ccagagatag gagtcaatca gctagtctga tcgactgttt taacaagttg
    47101 actttcattt taacttttga taccatttaa aacacaaatc gtaattgttt cgatttgtgt
    47161 tttttagcat gactacaatc aaagaagaaa gtgggtcgtc catatggaaa aaacagatat
    47221 ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa acacgaaaac gtgcgctgaa
    47281 gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa gaagaaggaa acttaattaa
    47341 aaaaatagcc gaaaaacgtt tttcggctat tttcaataat cggtttttgt gtaaggtttc
    47401 cgaaagaact gctgttgatt ttccattttt aacatatgat aaattggtag aatctttctt
    47461 ttcaaattga taccattccc taattccagt tatatagtta actacagaac cacaaagcac
    47521 gtacgtttat aaaagtatta tctgtagtta tttttcaatt ccctttcaac ctcttatttt
    47581 gaccagtagt attgatcact ttacctttta acacctttgt tcgtttttga aacattttta
    47641 gtcaagaatg gaaacgtaac tacataatca aaggttttgc gaaatatttc atgaaatatt
    47701 tcatgtagaa gcgagcaata cggttgcaaa taaccattat cgtttaataa gcagttacga
    47761 aagctcccaa tatctctaac gttcagctca ctccatttct tttcttcaaa aaaatcagtg
    47821 attcgtatct ctttctattt gtcgaaaaag ttaactttaa aaagtaacgc cgaaaaaata
    47881 tgcaaaaaaa taaaaaagca gttactattg ctagaataac atcaaggaat aataagtttt
    47941 ggttttcaga taatagtcga taagttgtgt attggaattt tagatacagt aataaagtat
    48001 ttttcatgaa attttgtgag aatcactatt acaagtatct ttaataaaat taatggttaa
    48061 ggaggaactt aaagtagaat gtaaatgaat agttgtagtc agtcgattgt gatactagac
    48121 tcaaagttgc aggaataagt ttttcaattt gtgattggaa gataaaaaat gaaaaagaag
    48181 ggcttttttt tatactttgt tgattgtagt gtttattttc cctctgttta gccaagtaac
    48241 attttctatg actcaaccag tttcaagtga ggaacatgat aacctttttc gtgctacaga
    48301 agagtttaag aaatatcagg aaaatattgt tccggagact ccattagcta gtgagccaat
    48361 tactgatatt gatggaaacc aaataggatg gtatatacag tacgaagtta tggtggtaaa
    48421 taatgactca atagtaaaaa atacagtagg cttagagcta acaccgattg aaaaggtgag
    48481 ttcattgtta acgtatattt atactgatga aagtgacgag actgagatca ttttaacgga
    48541 ttactcaaaa attgctactg ataaaaagta tatattgtgg atgtagacac tagtgaaata
    48601 ctttatgata tagaaattga tgaaaattta ctagatgtag cacaagaaat cgatcatcaa
    48661 aagcaggaag ctattcgata tgctggtaga aaagggaaaa gaaaatattt attacgtgaa
    48721 tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa ttcaagagtc gctactcaat
    48781 taactcgact ccgacattta cagtttacag agaaggatcg atagaaaaaa ctgttttttg
    48841 gactaaagaa agtggaatgt ctttagctga agtagaagag tttttggatt atgttagtat
    48901 gcaacagtaa gggagttctt attatgaaaa attttgacac atattatgtt attgtttttt
    48961 cgatatcttt gctgttaata ttactaactt taatgcagta taaagagtat ccagagtatt
    49021 ttattttaac ttttgtatat ttagtaagta gctacacact tctatataaa ttcagaagtt
    49081 cttctagagt ttctaaagtt tgtttcttaa ttagcttatt gattctataa tatttttgat
    49141 ttatatagga aattaaaaat attatagaat caacactcag agaacgggtt ggtttttaat
    49201 agatttaata agtactgata atgaaatttc tcctggttgg gaaaacgaag ttggttaccc
    49261 tgtagaagaa ttttttcaag attactttgc ggagtatgcc gataggataa tgagagatta
    49321 cgtaacagat aaaactcaag aagtcatcaa tcaatcccag tcattaaatg aattatctga
    49381 taaggcgaaa gaagaagcag ttgccattat ttcttcaggg attagtgact atattgatat
    49441 gagtggtgaa atcaattatg gcgataaaga caaaattgta cgcatgataa aagcttttat
    49501 caaaactttt gatgtgaagg cagatcttaa gtttaatgat acttacctag taggaaaatt
    49561 aattaatatc cttgggacag agaaagatac ggaaacgttt atttcctctt tgtctatgtt
    49621 gtttacagat aagcattttt caactcagct tggtggagag gaactagtgg aaatctttta
    49681 accattacgc ccagcattaa taaaagtata tcaaaaaagt actcctgatg atttttaaga
    49741 ttggtttgag aaataatgtg aaaacactat ttttaatgtt aattatagat agagatgaac
    49801 gcgtcatatg gttaaaagaa tgaaaaagtc aaagatacac tgctatattt cactaaggat
    49861 atagcagtgt atctttcatg tcgtttcact ggcagttttt tataaaagga ggtgtgcatt
    49921 tcagttgctt ttctttggta gttagttgaa tcgtttaaga attaacggat gtggcagaca
    49981 ggtgctctgt ccaaaaacca gtaaaatctt tgatggttcc atctttattt agctgtgcct
    50041 gttcaaatac tgttttgttt ttaccgaatg gatcaattgc aataattgtt ccgatccccg
    50101 cacgtttggc agcaagtaat cctgagtaag catcttcgat aacaagacaa tcagccggag
    50161 ctaagctcag tttgtctgcc gctttaagaa aaatatccgg ttgcggtttc ccagggaaag
    50221 agccgtcgtc atagacaatc ttgtcccagt caaaccagcg ggctaaatcg aaaatatcaa
    50281 aataaaaagc aacattctct ttgacagtag ccgtggcaat cgttaatgga atctgttggt
    50341 cagtcagctg gtcaagagtc gcttcaagcc cttttgtcaa ctgaagctcg ttttcatttt
    50401 gcaagcaaag catacgataa tagctttctt tttcttctga caatacggcg atctcagagg
    50461 cggtcaaatg ctctgaaatg aaatgtttca aaatctccgt attcgtccga ccatgaatat
    50521 tttttaaaat atcctcgtct gataaaccat cttttgtatg atcatgtatc atatgcagcc
    50581 aagcagcttc atgcagatga gagtccaaaa acattgttcc gttgaaatca aatataatcc
    50641 ctttcatagt tacgctccta tagtggtatt ttcggttgaa tcagcaattt gcagatcgac
    50701 atattgtgca tagtgctctt gaacctcccg cgagatctgg cgatccgtaa tgagcagatc
    50761 gaaatcatgc aaattataaa acacataaaa atcatctcga ttgaattttt tattatcaac
    50821 gagtagatat ttcttacggg aattatttag agcgatctgc tgtgcttctc cttcttcggt
    50881 actgtaagta gagatcgctt cgttgtgaac gccatttgaa ctgataaatg cttttgtaaa
    50941 ctttagtctt cttaaattgt cgttggtgat cggtccgaca aatgcgccgg tatttttccg
    51001 gaaatcaccg ccaatcagta gaatttctgg tccatctgct tgagcaagaa tatcaaaaac
    51061 tggataatta tttgtaatga tccgaatttt cttgttcagt aaatgcgtcg ccaatagttc
    51121 gattgttgta cctggtccta agaaaatcgt ttctccctcg tgaatcaagg aggctgctgt
    51181 ggcagcaatt tctttttttt cttgaatctg cagagtggct ttttcagtgt gagaaagttc
    51241 ttgatctaaa gtgtaggaaa gactttgagc ccctccatgg acacgcagca gcttgcccga
    51301 tttttctaat tcatctaaat ctctgcgaac ggtcatatca gaaacatcta attgattgat
    51361 gatttcatta acggtaagga ttcctttttt attgaccatt tctacgatag taagcaatct
    51421 ttcttttttc aacattgtct tcacctctaa tttaaggata atctatcata taaacggaca
    51481 acagtcaatc taattcgaac aaaagtaaac atttagggtg gtttttatgt ttgttacaat
    51541 agattttatt gctgttttct ggtttttatg attatttgtg tttgtttttg ttgacaaatg
    51601 ttttggatgc gtttacaatg caatcagtta gaaagagcga aaactgatta tagatttgag
    51661 gagagatgta catgcttaaa ttaccagaag attttatttt tggcggagcc acagcagcct
    51721 atcaggttga aggagccact aaggaaggtg gcaaaggcgc tgttgcttgg gatgactttt
    51781 tagaggaaca agggcgattc agtcccgatc cggcaagtga tttttatcat caatatgcaa
    51841 aggacattga attgtgtgaa cgttttggtg taaatggtct aagattatcc attgcttgga
    51901 gccgcatttt tcctgatgga gctgggaaac caaatccaga aggaatcgct ttttatcatc
    51961 gtgtgttcga agaatgtaag aaaagaaacg taacgccgtt tgtgacgttg catcattttg
    52021 atacccctaa acgtttgttt gaccatggag attttttgaa tcgagaaaca atagaagcat
    52081 ttgtttccta tgccattttt tgttttcatg aattcaaaga agtcaaggtg tggagtacct
    52141 ttaatgaaat ctatccagtt gcaaccaatc aatatttgtt aggtgttttc ccaccaggaa
    52201 tcaaatatga ttttacaaag atcgtcgcct gtcttcataa tatgatggtt gctcatgccc
    52261 gtgtagtgaa ctatttcaaa gaaaacgaac ttccgggtga aattggtgta gttcactcat
    52321 tagagacgaa atatgcggca acagacgcgc cggaagataa gcatgctgcc tttttagacg
    52381 atgcattgtc gatccgtttc ttattagatg ctacctattt aggctattat tcaactgaaa
    52441 ccttgactgc tcttgatgag atatgtgaag caaatcaggc cagctatcat tttcctgaag
    52501 aagatttcgt tgaattgaaa aaagcatcca cgcgtaatga ctacttaggg atcaatcact
    52561 accaatgcca ttttgtcaaa gcttacgatg gagaaaatgc aatccatcat aacggtacgg
    52621 gtgaaaaagg gacctctgtt tataaagtga agggaatcgg agaacgtatt tataaagaag
    52681 gaattcctcg aactgattgg gactggctga tttatccaga aggtctatat gacttgttgc
    52741 tgcgaattaa aagcgattac ccacattaca acaaaatcta tatcactgag aacgggatgg
    52801 ggtataaaga tcagtttgaa gatggcatta ttatggatca gccgagaatc gactatttga
    52861 gagtctattt ggaatcctta agcaaggcga taacagccgg ggtaaatgtg aaggggtatt
    52921 ttttgtggtc gcttatggat cttttctcat ggacaaatgg gtataacaaa aggtatggct
    52981 tgttttatgt tgattttgaa acccagaagc gttacccaaa agaatcagct tactggtaca
    53041 agttggtcag tgaaaccaaa acgattattt aataagtgat aatggaggaa gaaaagatga
    53101 ataaagagga attacaaatg cttggttttg agatcgttgc atattcaggc gacgctcgca
    53161 gtacattatt gaaattattg aaagaagtaa gacaaggaaa ctttgaacat gttgattcgg
    53221 cgttgaaaga agccgacgaa aatttaaccc ttgcacataa ttcgcaaaca aaaattttag
    53281 cggaagaagc atctgggaaa gagatggaga tgggattcat ttttatccat gggcaggatc
    53341 atttaatgac cactttattg ctgagagatc tgattcaaga ctttattgta ctgtacagac
    53401 agaatcaagg ataggagaag aacgctatgg atggaatcat taaacaaatc gaaaaaggga
    53461 aaccattttt tgaaaaaatc tcacgtaata gttatttagg agccgttcgt gacggatttt
    53521 taacagcgat gccagctatt ttattttcaa gcattttcat tttgattgct gcggttcctg
    53581 agatttttgg ttaccaatgg cctgaatctg tctccacatg gctatggaaa gtctataatt
    53641 attcgatggg tgttgtaggg ttactcgttt cagcaaccac tgctcgctgc ttggctgatt
    53701 cgatgaatcg tcgcttaccg aaaaacaaag taatcaacac cacctctgtg atgctggcct
    53761 ctgtggtaag tttcctgttg ttaagcgtct ctccgattga aggtggattt tcaacggaat
    53821 atatggggac aaagggacta ctggcttcct ttgtcgctgc atttttaaca gttaatttat
    53881 ataagttttg tgtgctgaaa gatattacca ttaaaatgcc taaagaagtt cctggaacga
    53941 tttcgcaaac cttccgagat atttttcctt tctctttttc agtatttgcg gccgttttaa
    54001 ttgatttagg cattcgacaa atcttccaaa tctcatttgc agaagcaatc attacggtgc
    54061 tgcagccgct atttacagca gctgacgggt acttaggaat cgctattatc tggggagcga
    54121 tggcgctgtt ttggtttgtc ggtgttcatg gaccgtcgat cgtggagccg gcaattgcgg
    54181 caattatcta tgccaatgtg gaaaccaatc tgcaattgtt caattccggt gaacaagctt
    54241 ctaaagtact gacggtgggt ttagggaatt ttgtaggaac tatgggagga actggggcaa
    54301 cattggttgt tccttttatc ttcctactct ttgctaagtc taagcagttg aaggcagttg
    54361 ggaaagcttc ctttattcca gtaagtttcg ctgtcaacga accattgcta tttgcggcac
    54421 ccatgatttt gaacccttat ttttttattc catttttgtt aacacctgtt gtaaacgttt
    54481 gcctgttcaa gttttttgtc gatgtattgg gcatgaacag ctttatgtac gttttgcctt
    54541 gggcaactcc agcaccgatc ggtttggttc taggaacggg catgggtatc ttagcttttg
    54601 ttctagcagc ggttctaatc gtcgttgact tcctgatcta tctaccattt tgcaaagcat
    54661 acgatgctgt cttagtagag aaagaagcac aaaaagcaga ggtgatcgca accgaggaag
    54721 gtgcttctgc cgatgtacgt gaggaagtac ctgtggcagc gagtcaagtt atcagcgatg
    54781 caaataaaga aatcaatgtg ttggtacttt gtgctggcgc agggacaagt gcgatgttag
    54841 ctaatgcttt aaaaaaaggt gcatctgaat cgaaagcacc gatcagtgca atggcgggtg
    54901 cttatggttc tcattacgaa atgctgcagg attttgatat ggtggtctta gcaccgcaag
    54961 tcaattctta ctatgatgat atcaaagaag acaccgatcg cttaggaatc aagctggttg
    55021 cgacaaaagg agcagaatat atcggattaa cgagagatcc tgaaaaagct gttcagttta
    55081 ttctgaacca atttcaataa aggaggagcc ataatgaaaa tgggaatttg gttgcgtaaa
    55141 gctgttttat ttgcagctgt gttaggggct ttgggggctg tttcaaaaac tgttgaagca
    55201 tctgccagcg aatttattcg cggagcagat atttcgatcc tgtcagatat ggaaaaatct
    55261 ggtgcaaaat attatgaaga cgatatttca aaagatgcct tagagatttt aaaaaataat
    55321 ggtgtcaact atgttcgact gcgtttgtgg caggatcctt ataatagtaa tggagaaagt
    55381 tatggagcag ggacaaatga tctggaaacg acgattgacc ttgcaaaacg agcaaaaaat
    55441 ctgggactta aggtgctgct ggattttcac tacagtgatt tttgggttga tcctgggaaa
    55501 cagaatcttc ctaaagcatg gcaaggtctg acctttgaag aaatgaatac ggcattgtac
    55561 gactatacaa agaacgttct gtcggaaatg aagcagctgg acgtttatcc tgatatggtt
    55621 cagatcggca atgaactgaa tagcggaatg ctctggcctt atggcaagtc ttggggagaa
    55681 ggcgggggag aatttgatcg attggctgct tttttgaagt ctggtatcca agctgttcga
    55741 gacactcaac ctaaaacaac tccagtaatg ctccacttag ctgatggtgg cgatacagga
    55801 gcatttactt ggtggtttga tgaaatcacg agtcgaggtg tgtcatttga tttaattgga
    55861 gtgtcgtatt atccgtattg gcacggttca atgaatgaac ttcaaaccaa tatgaatgcg
    55921 atcagccaac ggtatggtaa agaagtgatc gtagttgaaa cagcttatgg gcatacaaca
    55981 gcgaatgccg atactatgcc aaatgctttt ggtgaagccg aagcagctgc ggggggatat
    56041 gaaccaacac cgacaggaca agcagagtat ttgctggatc ttgccgatcg gattcaggca
    56101 gtaccgaata atcgcggggc gggcttcttc tattgggaac ctctttggta caacggaaat
    56161 gtgagttggg caacacaagc aggtatgaat tatctcagtg ttcaatccac tatgggaaac
    56221 gaatgggaca accaagcgat gtttgatttt gctggaaacg ccttgccaag cttgcgtgcg
    56281 tttaaacaag caggggcgca aataaatcta gttaaaaatg caagtttcga aagcgatgga
    56341 ccaacgacca gtccttccaa ttggcaaacc tggtttcaac aaggaaccgc taagggtacg
    56401 atcaaaacgg aagcgggcac tttttttggg gactacaaac taactttttg gcaggatcaa
    56461 gcatacgagg catcagctta tcaaaatgtt agtggcataa gcaaaggaac ttacacattg
    56521 agtgcctatg taatgtctgg gggagagttt gatactaatc agttatatat caaaaattat
    56581 ggtggagcgg aactaaatac tgcggttgtt aaaacaagtg aatggacaaa aattacaatc
    56641 aaagacattc caattacaac aggtgtttgt gaaattgggg tttatagcaa ggcttcagga
    56701 aatgcttgga cgaacgttga ttttgtttca ttggtgaaac aatagactgc ggatcgtttc
    56761 gattcaagat cggaggttcc cttcagccac atcgttagcg tagaaggcag gaatcgaacc
    56821 tgcacctcgc ctttgccaaa aataaccaga aacgatttcc agtcattttt accaaaacga
    56881 gtaaagcacc agacttcatc tacactttgt attatgctaa agagagttca acgatttcgt
    56941 aagaagcaat gaaaaaattg caaaaagttg tgcagataac ataaagtttg tttaagaaag
    57001 ggtaacctta gaattccgtc gtgaatatga tttgagcact tagatggata gaaaaaagtt
    57061 ctcggaaaac agttcgtttt tcgagaactt ctttttatga taagaatgaa acaaattaac
    57121 ttatattata gtctcttttg gtagggaacc atcttatttt taatgaaaat caagcatttt
    57181 agctattgaa gaatgttgat ttatagggat tccccctcca tattatgttg gctctttatt
    57241 cccactctgt tttcaacgta aagtgtataa aacagaatgc gatactatca agggagttaa
    57301 aacaatatac agaacagtaa aacactataa ataaaaataa aagtgcaata aaagtgcaat
    57361 ttttgcacct aatcatactc tgtttatata tgaaaactag attctgaatc caatatttgg
    57421 ttaacgagag gcgttcgaat ttgaacgtct tttatatatt actttcaaat aaatatgagg
    57481 tagaatacta tctattttct ttttaaccca attgttataa tggagagtgt aaaatatttt
    57541 gtgtaaatag aaaaaaggaa gtcccttctg tagaatagag ttaccacaac acattcacag
    57601 aagagaggac ttccctatga acgattttac tacagaaatt ctaaagactc tagcgaacaa
    57661 aggcgatttg aatgaattat tccgtgtcca tttggagaaa gcagtcaata cgcttctcaa
    57721 aacggagtta acggctttcc tagattacga aaagtatgat cgcattggtt ttaacacggg
    57781 taattctcgt aacggctcct atgaccgtac ggtcaagacc gagtatgggg aacttcatct
    57841 ccagattccg cgcgaccgca atggtgagtt caagcaacag actgttcctg cttatagacg
    57901 gacgaatgac acgttagagg agaccgtcat tcacctcttc cgaaaaggta ttaccatgtc
    57961 ggaaatcgca gacttgattg agaaaatgta tgggcattac tacacgcccc aaaccatgtc
    58021 caatataaca aaatcattta cagaagaggt aacggcgttt aatgggcggg agcttcatga
    58081 ccgttatgct gctatttata tggacgcaac gtatattccg ttaaagcgga aaaccgtcgc
    58141 caaggaagct attcatatcg cagttggcat tcgcccggac ggatcaaagg aagtattgag
    58201 ctatgcgatt gcaccgactg aatccatcac gatttgggag gaaattttat tggaccttca
    58261 agagcgcggt ttgaaaaatg tcctcctgtt catcacggat ggcttaaagg ggatggtagg
    58321 agcgattagt cggttctatc ccaaagctcg ttttcaacat tgttgtgtac acgtttcccg
    58381 taatatcagt cataaagtgc gtgtcgatga tcgtaaggaa gtctgtgatg attttaaaat
    58441 ggtgtatcaa gcctcatcta aagaggtggc gttggaagca cgtggtactt ttgcggaaaa
    58501 atggaaaacc agctatccaa aagtggttga atcgattctt tcgaacgatc acttgctcac
    58561 tttctatgat ttccccttgg ccatacgcaa gagtatttat tctacgaact tgattgaatc
    58621 ctttaataaa caaatcaaga aatacagcca ccgcaaggaa cagttccaaa atgaagagtc
    58681 gatggaacgt ttcttagtct cgtcttttga tacttacaac caaaaattcc taggtcgcag
    58741 tcataaaggt ttccaacagg ccgaaggcga gctcgaacaa atgctaagcc aactgattga
    58801 gaattagaaa caatctgcag tagggaagaa tcatttacac aaaattattg acgctccctt
    58861 ataatgagaa cttttactaa cataatttat gataaactta ctatataatc atattaatta
    58921 aatagttagg agatctgctt atgtttatat ctactcttca aattcgaaat tttagaaatt
    58981 atgaatccga aatctttcga ttcgataatg agacaaatac tattataggt gagaatgatt
    59041 cagggaaaac caatgctctt actgcattga gaatcttatt agatgatagt tattactata
    59101 gtagtaaaac actaaaagaa tcagattttt ttcatggaat tcaaaatggg tggcaagggc
    59161 attggattat tatctctgcg acatttgaag gaatcagcga agaagaattt gacaatgaga
    59221 tttgtgcttc catttcttta aattctgaaa gtcaaacaat cttagaagaa ttgatttcaa
    59281 atgcagataa aggtgtaggt agtatttctc tatttattag acctaacaaa gctattagaa
    59341 agcaattatt tgatatttcc actgaattag actctcatca atttaacgaa tttagaaatt
    59401 ctattcgtct atccgactat gaattttact acacatctaa atcaaatcta aatttctgta
    59461 tcaatgaaaa ttatgataaa ttggtaggaa gactcaatga caatatagct agtaatcctg
    59521 aagaagacga tgaagctctg ttgggctcaa gaattgacat gtcagatatt ttcaagcata
    59581 tttcggttgt ttatgttgac gctcttcgag atgttcttag agaaatgaaa aacaatcgaa
    59641 acccagtcaa aagaatcatg gaaacaattg aatcaaaaat atcatcagat aatgttgagt
    59701 cattaaaaac aattatacag caattaaatg agactattac cagtgttcct gaaattagga
    59761 aaattggcga aaatattaat cggcaactaa atagcataat tggttcagtg tattctccta
    59821 atcttcttct ttcctcaact atgtcagatg atatgggttc tttagcaaaa tttctttcaa
    59881 tgaagccaga acaaaatatc gacttagact tactagggtt aggacatcta aatatgattt
    59941 atttagcttt gaaaatagtc gaatttgaag cgtgtcgttc aagagaacta cttaatatta
    60001 tgttgattga agaaccagaa gctcatattc atcatcatat tcaaaaaact ctgttcgaag
    60061 gattaaatct tcaaaaaaac tatactcaga ttctcatgac cacacattct gtacatttag
    60121 ctgaagtgtc tgaaatatct cggatgaatg tattaaaatc gtttaatggc aaatctatcg
    60181 ttatgaatcc agcaaataat ctgaactcat ttgctaaaga aaagttaaaa aagaacaatc
    60241 taaatctaat aaacgcagta gagagatatt tagatgttaa aagaaatgga ttgctttttt
    60301 ctaaaggtgt tatcttagtt gaaggagatg ctgaagaaat acttattcct caaatagtaa
    60361 aaaaattatt tggtataaac ttagacgaac ttggaatagg tttgattaat gttggtagta
    60421 cttcgtttga atatatcgct agcttgtttg acgatgatag aatacagaga aagtgtgcta
    60481 ttgtaactga tttagatgaa caggcaatcc catcagaaca tcgattgtat aaaagcactg
    60541 cacaagagaa aggtcaagca agaaaagaaa aattatcctc cctttttaat gaaaacaatt
    60601 gggttaattg tttttatgct aatacaacat ttgaaattga gtttttagat gtgaataaag
    60661 aaaatattct aacttatgtc agtccaatta tagatgacgc attcaaagat aaaaaaacaa
    60721 taactgctta taaagaagaa atacaaagtg aacaatattt agaaagaaat ttagcgatgt
    60781 taaaactagc caatcatgtt ggtaaggggt ggtttgcaat tgagttaggt gaaaaaattg
    60841 actttttaat caaaattcct aagtatattc ttaatgctat tgcttttgca tgtcaggaaa
    60901 tagtagatct ctcaatttat aaaaaaattc tactccatac aatcgacttg tacgaggaaa
    60961 ctacagaatt gtcagcttta tcagctagtt taaaaacaag tgatacagct gatttgaata
    61021 tagcaataat ttctttatta gagtactttg gtaaagatga ggatgttgtg tatctaatca
    61081 atcagataga aaattacaca aaaatacttt atgctgaggt ttcaaaatga caaatatctt
    61141 cgaaaaaatc gtaaatgatt taaatttaga acaaaaagat gttgtggaaa gtgaagataa
    61201 cctttatgta aatgcatgtc ctgggagtgg gaaaacaagg gtacttacaa ggaaaattgc
    61261 ttaccaagca attaaacacg atggatcact aaaaagaata attgctatca catacactaa
    61321 tagagctgct gaagaaatta aggaacgcct aagtttactt agtatagatg atgatattaa
    61381 tatttgggtg gggacaatac atcagttttg tcttgaattt attatttatc cttttaaaat
    61441 gaatctctca agagtatcaa aaggatttac aattattgat gattatactc aaagagctta
    61501 cgtcagccaa attaataata tgctgaattt aaatctcaaa acgtatgaac tgaacaaaat
    61561 tgatttgtca ctttctcatg attttaaaat taatgaagtt cagtatactc aagtttctga
    61621 aatgtatcac actatgttgg aaaaaaataa agaaattgat tttgatatga ttttactagt
    61681 agcgtataaa attttaactg taaacagtaa cgttgcacgt aatattgcta gtaatataag
    61741 ggcgatttat gtggatgaat ttcaagatac tagagaatta caatacaatg taatagctaa
    61801 attacttcaa aacaaccctc aaattcagtc aatgtttgtt ggagatattg accaagcaat
    61861 ttatggatca ttagatggta tagctaaatc agttgaagaa ctagaaatgc taactagtca
    61921 cacttttcaa tcaaagacgc tacatggctg ttacaggtct aatcagagat tagtagactt
    61981 ctactctaat tttcagagtt gtccttacga gattgaaagc agagggaata ataaaaacga
    62041 ccgtggtttt ataagttatg attgcaaagt tactaaggag gatttaccca acataataaa
    62101 aaatataata aaggaaaaga ttgaaagcgg cattcatgaa aaagatattt gtgtacttgc
    62161 tcctcaacac tatcctctgt tttcactggg tgagtattta aagaaagaat taccgtattg
    62221 caattttgat gctataaata tatcaccaat taaggtaaat aatcatagtt tatttttcaa
    62281 gttagcaatt ttgtacttta ctgaatcagg tgaaaaggtt cgaagaagaa agctaattgc
    62341 aaacgatatt ttaattatat taaaaaatga atttggaata gaagttaaag attattttat
    62401 tgacttagat ttattacagc taataaacag tgtgaaaaga atgtatagtg gagacgacaa
    62461 cggtgttgat ttatacaaat ttgttaccaa acatatattt gttaatctta atattgacga
    62521 gaaaaattac ccgtctattt ttaagcacta tctatttttt attaacgaag tagaagatcg
    62581 gataaaaaat aattcattat ctagtagtgt atctagtttt aagaagatgt ttaaatctcg
    62641 agatggtatc acacttacta cagcacataa agttaaagga aatgagtatg atactgttat
    62701 cgctattaat cttctaaacg ggaaaattcc tcattggaat gaaattttta atgtttctga
    62761 taaaggaatt agttcagcga gaaaactact atacgtaatt tgttcaagag caaaaaataa
    62821 tttatttctt ttctcagagg tgggttattt tacgagtaaa ggagacttgc tttatcccac
    62881 tcaattatta gataaaatag aatttgatta taatatttaa aatacctagg tagtcgttgt
    62941 tttaaatata gtaggaatcg gacgctcagt acggagcgtc tattttttta cccaattttg
    63001 aaaggaagtg aacttatgaa aataaaaaat caagaatcaa aaggtcgctc tccccccttt
    63061 aaaaccatca cacaaataag attcattcaa acataaaaac agaaaggata ggtaaaaata
    63121 tggaacttaa atatgtcatt cccaatatgg aaaaaacatt tggacaatta gaatatgctg
    63181 gtgaagggaa cattgaacaa cgtcgaataa atggcagacc aaccattctc tctcgtagtt
    63241 acaatttata ctcaacgatt caacgagcag atgatattgt tgtcattctt ccctccgaag
    63301 ctggcgagaa acattttgaa tccgacgaaa aagtcaaact catcaaccca catattaccg
    63361 cagaaggtta taaaattggc aatcgaggat ttaccaatta cattatgcac gcagacgata
    63421 tggtgaaagc ataagaaagg aaaggtgaaa aaatatgaga ttagcagaag gtattgtgat
    63481 tgaaaaagac cagacatttg gcacattgaa attttccgcc ttgcgtcgtg aggttcgtat
    63541 ccagaatgaa gatggtacgg tttccgaaga aattaaagaa cgtacctatg acttaaaatc
    63601 aaaaggacaa ggacgcatga tacaagtcag tatccccgct tctgttcctt tgaaagaatt
    63661 tgactacaac gcagaagtgg aattaatcaa tcctgtggca gataccgtag cgacggctac
    63721 ttttcgaggg gcagacgttg attggtacgt gaaagcagac gatcttgtat taaaaggaca
    63781 gacaaaacct catcagaaat ataaaaaaga agcgtccgaa agtgaaaaat aggtgttgct
    63841 tatgacaaac acaccattga ttttgaaaga aattccgaag gatgaagcca tttcttttat
    63901 tcggcaatac cattattcaa agatactccc tagattgtgt aaatactttc tagggatttt
    63961 ttcagaagaa aaattattag gcgttgtgga attaggctgg ggtacgcaac ccctacaaac
    64021 catacgcaaa ctctttcccg acagttccct tcaaaccaca gattatttag aaattggaaa
    64081 gatgtgtttt cttccagaaa tgaatcaaac caattatttt ggcagtcaag ctctttcagc
    64141 tctaattaag tggttgaaag aacatacgga ctgtcatttt ttatatacac tggcagatgg
    64201 aattgaggga aaatgtggct atgtctatca agcgtccaac ttcttttact gcgggtattt
    64261 caagacaagt gtctatcgtg ataagcagtc atgggaaaag atacaccctc gaagtgctcg
    64321 ccttttatta gaggaaaatg ctcgctttga acaggtagag aaaaagcatt ggctttccca
    64381 ggcattttgc gaatacaaag ggattgagaa aatcaatgga cgcatgtttc gctatctcta
    64441 tccgttgaca aaagaagcga aaaaactgtt aggacacact ctctatcgac gtcattatta
    64501 tccgaaagaa aaaaatttac gctttgaaaa gcgaatcgct tatcagaaat atgaagccat
    64561 ttctcaacca acttttgata aacaggctcg tatctacaat acacaattat tttgataggt
    64621 ggaaaggagg atttttatgc cattgcttca tgacagagga gagcgaatca aagccaacga
    64681 taagcacttg gtctatcatt ttgtactaat ttcattgctg gtagtttttc tagtcgtcct
    64741 actccctttt cattggagag cgttacaatc ggtgaactgg caagcagttc agttagatac
    64801 atttttctat caacttcata cgccatactt ttggagcagt attctcactg ctctttttgt
    64861 atgcggactt atcagctggc tttattatca ctatcgtata gattctataa aacagttaga
    64921 gcaccgtcaa aagttagctc gtatgatttt agaaaatcat tggtatgaag ccgaagaaac
    64981 tcaatcagag ggattcttta aagatttacc cagcaagaaa accaaacaac gaatccgcca
    65041 ttttcctaaa atatattatc gaatgaaaga tggactaatc catattcatg tagaaatcac
    65101 cttagggaaa tatcaagatc agcttttgca cctagaaaac aaactagaaa caggattgta
    65161 ttgtgaatta gtggaaaaaa cgttgaaaga ttcttatgta gagtatgtcc tactttatga
    65221 tactattggc aatcgaattg gcattgaaga cgtggtagct caagatggac gtgtcaaatt
    65281 gatggaatcg gtctattggg agttcgattc tctccctcac atgttaattg ctgggggcac
    65341 tggtagtgga aaatcctatt ttattctcac attgattgaa tctctcctgc atacaaatgc
    65401 aaagctttat attcttgacc ctaaaaatgc tgatttggca gacttagggg cggtcatgaa
    65461 taatgtctat taccgtaaag aagatatgtt ggcatgtatc aatcaatttt acgaagatat
    65521 gattgatcgc agtgaaacca tgaaacaaca ccctaactat aaaacaggag aaaattatgc
    65581 ttatcttggt ttgtctgcca attttcttat ttttgatgaa tatgtggcaa tgatggatat
    65641 gctaggtcgt gaaagtacca ccgttattca taaactcaaa caaattgtaa tgttaggacg
    65701 acaagcaggc ttctttttaa ttcttgcctg tcaacgtcca gacgcaaaat attttagtga
    65761 tgggattcgg gacaacttta acttccgtgt ggctttgggt cgtatgtctg aaatgggctt
    65821 tggtatgatg ttcggaagcg atacacaaaa acaatttttc ttaaaaccca tcaagggaag
    65881 aggttacgtg gatacaggaa aaagtgtgat tagtgagttt tatacacccc ttgtaccaaa
    65941 aaggtacgat ttcttaggag aaataggaaa agtcattcaa aaaaagcaat ctgagcctgt
    66001 accgcacgaa gtgaaaggta caggttcaga cttgctttga ttggtgtggc gttagccacg
    66061 ccaatccttt agccccctct atctaacagg ggggcacaaa tacaactgaa aacaggcaat
    66121 tctcatcaac caatgttgat ggtacaaagg attgcggaaa atatgaaagg tgtaaactca
    66181 agggtcatag tttacatctt ttttagattg gaggtttgcg aatggaaaac gaaaaaattt
    66241 ggattaagga attgaaagaa aagcgactgg cttatggcgt ttctcaaaat aaactggcgg
    66301 ttgcttctca tattaccaga ccttacctat cggatattga aacaggcaag gcagtcccta
    66361 ctcaacaggt gaaagaagat ttactgaacg ctttggaacg cttcaatccc gataacccat
    66421 tggagatgtt attcgactat gtacggattc gattccctac caatgatgtg gctcaaatta
    66481 tcggtgaggt tttacgtttg aatatggatt atatgctaca cgaagacttt ggctattatt
    66541 cttatcccga acactatcgt ttcggtgata ttgttgtcct agtctctcat gatgtaagta
    66601 aaggtgtttt attagaactg aaaggaaaag gctgtcggca atttgaaaac tttctattgg
    66661 ctcaacatcg gagctggtat gatttctttc aagattgcat ggaacataaa ggtattttca
    66721 aacgcttgga tttagctatc aacgataaaa cagggatttt gaatattcca gatttggcaa
    66781 agaaatgtaa acaggaagaa tgcatttccg tctttcgaag ctttaaaaat tatcgcagtg
    66841 gtgaattggt tcaccgtgat gaaaaaccat acatgggaaa cacactttat attggctcat
    66901 taaaaagtga agtctatttc tgtatatatg agaaagatta tgagcaatac gtgaaaaatg
    66961 atgttccctt agaagacgca gaagtaaaga atcggtttga aattcggtta aaaaatgacc
    67021 gagccaccca agccatgaaa gatttattgg cacatcaaca ggcggaaaaa acagcctttg
    67081 aaattatcaa tcgctatatc cgctttgcag ataaagacga cactaaacgt cggagtgatt
    67141 ggaaaactaa tgaacgttgg gaatggttta tcggtaaaaa tcgtggagca ttacgcctaa
    67201 ccacacagcc agagccttat tcttttgaac gaacattgaa ctggttacat catcaagtcg
    67261 cccctacatt aaaaatcgca agcattttag atgttctcaa cggtacaacc attatctcca
    67321 ccatgattca ggaagccaaa ctgacagaaa aacatgagaa attaattgaa caacaacatc
    67381 tagccatgga ggatttgatt acatgagcct tttaacaaaa gaattaaaga agttagggtt
    67441 tcagtgtggc attgagtttc aagcgtatat acaaaataca gggaaatata cgtctctcat
    67501 tattgaggga aaacggcaag ctggtgatac catctatacc tatgattttt acaaggtgag
    67561 attttataat aattatacca atcgagtaac cgtctatgga gagcatttaa ccccttttca
    67621 attattaagg cgtgtaaaaa gctatatcta ttatcgagaa aaatatctta aggaaaggag
    67681 gacaatcaca tgaattttgg gcagaatttg tacaactggt tcttggataa tgcacagtca
    67741 ttggtattgt tagcgattgt tgtcattgga ctgtttttag gttttaaacg agaattttca
    67801 aaactcattg gcttcttgat tattgctcta atcgccgtcg gacttgtgtt caatgcagct
    67861 ggcgtgaaag atgtgttatt aaacttattt aaccgaatca ttggtgctta acagatattt
    67921 ttcatgataa aataggaagt atatattttg ttatgaaagg atttatgaat atgtttacaa
    67981 atgatcaatt taaatctctt ttaattaaaa cattagaaag aaagagctct tcaaattatt
    68041 atgaaggagg taacgagatt gtttctaaaa tgaaaatatc agaggtaact ttagatgaga
    68101 ctgacgattt tacttattat aaaggatata aaaagggctg gaatacgctt tgcacatatc
    68161 ttaaaataag agttcctttt gatgatttag acttttttga aagtcataaa gatttgatta
    68221 cccagacagc aagctccata tatgataaac aaggtgataa tgtattagtg gatactattt
    68281 tagtaccact tccagagaat tacgaagtaa ttaactttag tcagttgaaa attagtgatg
    68341 tggtttcaca agctattgaa gacgctgagt cttttatgag taatggtgaa tatcaacgtg
    68401 cctttgacag agttcacact gcttttcatg gatatttaat tgaaatacta aaaaaatatg
    68461 aaattacagt tcctagagac gaaaatctat ctaaattata tagtaggata caacaattaa
    68521 ttgaaaaaga aattcaacct actgaacttg ctgatatagt gaagacaacg ataaggtcat
    68581 caaatggaat gattagctct cttaatgaag caagaaatag gcattcactt gcacacccta
    68641 atacaaatat tattggaaag agagaagcaa aactaataat aggtatttca agtacagtta
    68701 cagattatat tagcggatac cttgataagt agcgtcatta actgattgct acttttttta
    68761 tgactatttt tataaaaagg atgtgataag aattggaaat gaaagtctat gtggcaaatt
    68821 taggacggta taatgaaggt gaattagtcg gagcatggtt tactccacca attgatgagg
    68881 aagaaatggc agaaagaatc ggtttaaatg aggactacga agaatatgca atacatgatt
    68941 ttgagcttcc ttttgatgtg gacgaataca cgccgattag tgaaatcaac cgtctatgtg
    69001 aagccattca agaaatcgaa ggtacaccta tttataatga actcaaagaa attcaaggca
    69061 tgtggtttag tagcttggaa gaattactgg aaaacaaaga ggatattcat tgttatagtg
    69121 attgtgattc gatggaagat gtcgcccgtt attacgtgga agaaacagga cagcttggtg
    69181 aagtcccttc caatttacaa aattatattg actaccaatc tttgggacgt gatatggaaa
    69241 tagagggtaa ttacctagtt acctctcatg gggtatttga atattgtcag taagtttaac
    69301 aatccgtgtt ataattaaat taataaatag cacggagagg tataaattat ggtaaatgta
    69361 tttgaaaatc ttaaactatt aaaactagac atgaaagata aaggctgggt gatagattcc
    69421 ttttactttc gttacaaaca gcaaaattat attgtacttg ttaagctatt tgaaaaagag
    69481 gaaaaagttc cagaatatgc tctgttaaaa ctagaatttt taaaggaaaa cgatttttca
    69541 gatatgctag ctgtctatgc taattcggta aaattatata cagacacgaa aacaataaga
    69601 gaatattttg gtatcgaata cagtagtaac ttgggagatg ttttatttca atttagccaa
    69661 actcttgctc gatttattcc gacagaagtt agtgagaaga aaaatgaaga ccaaaaagaa
    69721 gccatgtgtt tctcattaag tcaaagtgat tcagaagatc ccagaaaaaa atattgtttt
    69781 tcagttagaa gaaatccttt aaagggaaat ggtgagctgg gaaaacgaag tccttttaat
    69841 gacaataaaa cgagattgta tagacccagc ttatatgaac gattagggtc agataccaat
    69901 ttaagtttca gatattcaat gaatccagat gatgaagaaa ctgatgaagg tattattgca
    69961 aaatggacaa aaaataaaat tgaatagtta aagcattgaa agagcaatca cctattttga
    70021 ttgttctttt tctattttta cggaaaggac gtgaagacat gaaaaaaata agaagttaca
    70081 ccagtatttg gtcagtagaa aaagtgattt atgcgattaa tgatttacaa ctccctttcc
    70141 caattacctt tacgcaaatg acatggttta tcgtttcttt gtttgtcgtc atactctttg
    70201 gcagtttacc accactttct ctgattgacg gagcactact caaatactta gggattccca
    70261 ttgttctcac tgggttcatg tgccaaaaga cctttgacgg aaagaaaccc tatggatttc
    70321 taaagtctat cattacctat gctctacgcc ctaaagtaac ctacgctggc aaacaaatac
    70381 gattaaaaaa agaaacaatg aatgaatcga ttacgattgt aaggagtgaa caactagatg
    70441 gcatatccga ttaaatatat ggaagataat ctcgtattta atcatgatgg cgaagtattc
    70501 gcttattatg aattaattcc ctataattac agttttttaa gttccgatga aaaaattcaa
    70561 gttcatgata atttctgtca attgattgcc caaaatcgtg atggcaagat tcatgctctg
    70621 caactagcaa ccgaatcaag tattcgagcc acacaggaac aatcgaaaaa agaaatcact
    70681 ggacgattaa aagagatagc ttacgaaaaa atagatgaac agaccgaagc gttaatttct
    70741 atgattggtg acaatcaagt agactatcgc ttttttattg gttttaaact ctacctcaat
    70801 gaacaggaag tatccgtaaa aaatgttggg aaagaaattc aaaaagcatt tgcggaattc
    70861 tttcatggtg tgaatcacaa attaatgggc gattttgttt ctatgcctat ggcggaaatc
    70921 gaacgctttt ctaagatgga acagttgtta gaaagtaaat tatccagacg ctttcagatt
    70981 cgtaaattgg agaaagatga ttttggttat attattgaac atctttacgg gcaaacaggt
    71041 gtcccttttg atgactacga ctaccattta ccgaaacgat acgaagataa aaaagtgttg
    71101 gtaaagaaat acgatattct caaacccact cgctgtttaa tcgaagaaaa tcaacgctat
    71161 ttacggattg aacaggaaga taccactact tatgtggcgt attttacaat taatagtatt
    71221 gttggtgaat tggatttccc taatagtgaa attttttatt accagcaaca gcagtttact
    71281 ttccccgttg ataccagtat gaatgttgaa attgtcgcca ataaaaaagc cttatctacc
    71341 gttcgcaata aaaagaaaga actgaaagat ttagatagcc acgcatggga aagcgacaac
    71401 gaaaccagta ctaatgtggt ggacgcttta gaatctgtcg atgaattaga aagtaattta
    71461 gaccaaacca aagaatctat gtacaaacta agctatgtgg tacgggtatc agcaaagaat
    71521 ttggacgagc tgaaacaacg ttgtgatgaa gtaaaagatt tttatgatga tctgtctatc
    71581 aaattagtac gaccttttgg cgatatgcta ggcttgcata atgaatttat tccctcaagc
    71641 aagcgatata tcaatgacta tattcaatat gtcacgtcgg attttttggc tggtcttggt
    71701 ttcggtgcta cacaaatgat tggtgaaaat tctggtattt acgtcggtta caatctcgat
    71761 acaggcagaa atatttatct ccaacctagc cttgcaagtc aaggaattaa aggctctgta
    71821 acaaatgcat tagcgtccgc ttttattggt tcattaggtg gcggaaaatc attctctaac
    71881 aatatgctgg tctattattc agtactcttt ggcggacagg caaccattgt tgacccaaaa
    71941 gctgaacgtg gtgaatggaa agaaacctta cctgatattg cccatgaaat caatattgtt
    72001 aatctcacga gtgagaacga caataaaggg ttacttgacc cttatgtgat tttaaaactc
    72061 ccgaaagatt cagaaagcct tgccattgat attctcacgt tcttaacagg tatttccagc
    72121 cgagatagcg agaagtttcc tgtcctgcgt aaagctattc ggagtgtaac tcaaagcaaa
    72181 caacgagggt tgttattggt gattgatgaa ttgcgaaaag aagatacgcc catcagcaat
    72241 agtattgctg accatatcga aagctttgtc gattatgatt ttgcccatct gttattcagt
    72301 gatggcagtg tcacccaatc cattagcttg gaaaaacaac tcaatattat tcaagtggca
    72361 gatttggtct tacccgattc caatagctct tttgaagaat acactaccat ggaattatta
    72421 agtgtggcaa tgctgattgt cattagtacc tttgctttag actttatcca ctctgaccgt
    72481 tccattttta aaattgttga ccttgatgag gcttggagtt ttttacaagt ggcacaagga
    72541 aaagctctat ctatgcgatt ggttcgagca ggacgtgcca tgaatgcagg ggtctatttc
    72601 gttactcaaa acgcggacga tttactagat gaaaagatga agaataatat tggcttgaaa
    72661 tttgcctttc gttccactga cctagtggaa atcaaaaaga ccttagaatt ttttggtgtg
    72721 gacaaggaag atgaaaacaa tcagaaacgc ttacgtgaat tagaaaatgg acaatgttta
    72781 gtttcagaca tctatggtcg tgtaggggtt atgcagtttc acccgatttt tgaagagttg
    72841 cttcatgcct ttgataccag accgcctgtg cgaaatgagg tggaagaatg acacaaaaac
    72901 aaaagaaatt tcttcactat agttggataa cgctcctttg tgtgggcgtt attttacttt
    72961 tattaggcac gctgggcaat gcagtacaag cgacaggctt agttgatgaa accaaatact
    73021 ccaagtatgg tttgaatcaa tatcagcttg attattatgt ggacaatagc tggggctggc
    73081 ttccatggaa ttggagtgat ggcataggac aatcggtgat gtatggattg tatgccatta
    73141 ctaattttat ttggacaatt tctctttatc tatcgaacgc cacaggttat ttggtgaaag
    73201 aagcctatgc cttagatttc attagtcaaa catcccaagc aattggcgaa aacatgcaga
    73261 cgattgcagg cattacaccg aatggctttt ccagttcagg gttttatgtc ggtttcctcc
    73321 ttctttttat tctcattgtt ggaatctatg ttgcctatac aggattggtt aagcgagaaa
    73381 cgacgaaagt gatacgagct gttttaaact ttgtcgtggt gtttattcta tcggcttcct
    73441 ttattgcgta tgcacccgat tatattgcga aaatcaatga tttttccaaa gatgtcagta
    73501 ccgccagtct gaatattggg acaaaaattg ttcttcctaa cacggatagt caagggaaag
    73561 acagtgtgga catgatacgt gacagcttat tctctgttca agtaaagcaa ccatggctat
    73621 tgcttcaata tggctccact gatgtagaag cgttagggga agaacgtgtg gaaagtttat
    73681 tgtctaccag tccagataca aacaatggtg aagatagaga aaatgtagtg attgaagaaa
    73741 ttgaagatca tgacaatacc tatttaaccc taccgaagac gatttccaga ttaggaacag
    73801 tcttcttttt gtttgtattc aatattggta tctcgatttt tgttttcctt ctaacaggaa
    73861 tcatgatttt ttcgcaagtg ctatttatta ttttcgctat gtttttgcca atcagtttcc
    73921 tattaagtat gttgccgagt tttgatggca tgggcaggca tgccattacc aagttattta
    73981 atgtgattat gacaagggct ggtattacct tgattattac cgttgccttt agtatttcca
    74041 ctatgcttta ttcgctgtca gcaagttctc cctttttcat gattatgttt ttacaaattg
    74101 tgacctttgc agggatttat tttaaactgg gcgatttaat gagcctattc actttacaaa
    74161 gcaacgactc tcaaaacatg ggcagacaag tgtttcgcag acctcgtcaa atgatgaatc
    74221 gacaaaccag aagatttcaa cgcagtatga gtaagatttt tcgtggaaaa gcagggtcaa
    74281 cgaagcaaaa gacaatgaca acaaagcaga atcaaaccaa taaagctaac catactcgtc
    74341 aaaatgaacg gactacacca aagaaagaaa cagcactttc caatgtgaaa aagcaacaag
    74401 gcggtatcgg gcaacgatta ggtgaaaaga cagcccatgt attggacacg aaaaatcgtc
    74461 tagtcaataa aagaaagcag gcaaaacaac agatacaggc gacaccaacc catgtcaaat
    74521 atgcccttca tcaaggaaaa gagaatgtca ctcataatgt aacagacttt aaacaatcta
    74581 tggcagatac caaagctcaa cgcaaacaac agcgtatgaa ccagcaggag caaaaacgaa
    74641 agaacattgc tcaaaagcgt ttggagctgg ataaagcaaa agaaaaaaga cgaaaagaaa
    74701 acccatcatc tcctgttcag aataaccagc agcatgttcg caagcgtcag caaaccgtca
    74761 cgcctgtttt gtcacaaaac actaaacgtg aaaatcatca gcgaccattg aaaaaagagc
    74821 aaaaacaaaa gacaccaccg aagcaattca atcagaggaa gctaaataaa agaacggata
    74881 agcgaggaca acaaacatga agctaaaaat aacagtgatt tgtagtgtgg tcttctctct
    74941 cttctttttt ctcttattat tcctagtcat tttctttgca gatgatacgg acagtggcga
    75001 aaacaacaag gattcttcta ttccacaagg cggtgtgacc gtttcaccag aagtgttggc
    75061 acaccgtccg ttgattgaaa aatatggtaa ggaatatggg attgaagact atgtttctta
    75121 tatcttagcc attatgcaag tggaatcggg cggtacagca gaagatgtta tgcaaagttc
    75181 cgaatctttg ggtttaccgc ccaacagttt gagtaccgaa gagtccatca aacaaggggt
    75241 taaatatttc agtgaattat taaccagtgc agaacaacaa ggagtggata tagattctgt
    75301 tatccaatct tataattatg gcggtggctt tttaaattat gtgaggagtc atgggaaaaa
    75361 atatacctat gaattagccg aacagttttc taaagagaag tcgggtggtc aaaaagcaga
    75421 ctatcccaat cctatagcca tacctgtgaa tggtggttgg cgatataact atggaaatca
    75481 gttttatgtt cagttggttt cacaatacct aaccgacact tccccaacag aatttgatga
    75541 tgaaaccgtg caagtcatta tggacgaagc cttgaaatac gaagggtttc cctatgtctt
    75601 tggaggggcg tcgcctacca cctcttttga ttgtagtggc ttaatacaat gggtgtatga
    75661 caaagctggc atttctctcc ctcgtgtcgc ccaagatcag tatgacgcta cgcaagaaat
    75721 ctctatggaa gaagcccaag caggcgacct tatcttcttt cattcaacct ataatgcagg
    75781 cacatatgtg actcatgtgg cgatatattt agagggaaat cgcttttatc atgcaggcga
    75841 cccaatcggt tatggcgact taagcagtcg ttattggcaa gaccatctga ttggggctag
    75901 acgtgttatt cataactaag aaaggaatga atcaaatgat tataaaaatc gagcgaaaag
    75961 aaaagaagga gaaaccaaag aaatctaaaa aaccgagcaa gcaaagaaaa gttccgatga
    76021 ttaaagttgg cacgcataag aaattaacct ttgttttatg ggttttattg attggtagtg
    76081 tcggatttgg aatctataaa aattttactg ccattgacac tcatactgtc cgtgaaacag
    76141 aaatcattaa acagcagatt gtcgatacca accaagtaga aagttttgtg aaatcctttg
    76201 cgaaagacta cttttcgtgg caacaatccc aagaagccat tgataaacga aatgaaaaat
    76261 tgacccatta tttgacggaa gaattgcagg tattaaatga agaaatgatt cgtaaggata
    76321 ttcctactag ttctagtgtg aacgatatac aggtgtggca ggtttctcaa gtgaatgaga
    76381 acacctttga ggtactgttt tctgttgaac aagttattac cgaagacaaa gacaaggaaa
    76441 ctatatcgtc tagctttcat gtggttgtcc atatagatga atcagataat atggttatta
    76501 tcaaaaatcc gacaatgagt aagaaacctc aaaaatcgga ctaccaaccc aaacagctag
    76561 aaagtgacca tacagtagat acagaaacga tggacgaaat cactagcttt ttagaaacgt
    76621 tcttccagct ctatcctacc gccacagaaa aagaattgac ttactatgta agtaatcatg
    76681 tgttacctat gattaacaag gaatatgttt tcgaggaatt ggtgaatcct atctttacca
    76741 gaaaagataa ccaagtaatc gtgaatgtcg ctgtgaaata tttagatcaa gaaacaaagg
    76801 caacccaaat ctcgcaattt gagttgatat tagaaaaaca agataattgg aagattgtaa
    76861 aataacaaat attggtacat gattacagat actttgtaat catgtactct ttttgataaa
    76921 aaattggaga ttcctttaca aatatgctct tacgtgctat tatttaagtg actatttaaa
    76981 aggagttaat aaatatgcgg caaggtattc ttaaataaac tgtcaatttg atagcgggaa
    77041 caaataatta gatgtccttt tttaggaggg cttagttttt tgtacccagt ttaagaatac
    77101 ctttatcatg tgattctaaa gtatccagag aatatctgta tgctttgtat acctatggtt
    77161 atgcataaaa atcccagtga taaaagtatt tatcactggg atttttatgc ccttttgggt
    77221 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
    77281 gc
//
LOCUS       DANKEF010000007        74520 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000007 DANKEF010000000
VERSION     DANKEF010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74520
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.007"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            159..3437
                     /locus_tag="PWZ48_000524"
     CDS             159..3437
                     /locus_tag="PWZ48_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HDL2260204.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(3978..5885)
                     /locus_tag="PWZ48_000525"
     CDS             complement(3978..5885)
                     /locus_tag="PWZ48_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HDL2260205.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            6015..6890
                     /locus_tag="PWZ48_000526"
     CDS             6015..6890
                     /locus_tag="PWZ48_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HDL2260206.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            6970..7944
                     /locus_tag="PWZ48_000527"
     CDS             6970..7944
                     /locus_tag="PWZ48_000527"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2260207.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            8124..9269
                     /locus_tag="PWZ48_000528"
     CDS             8124..9269
                     /locus_tag="PWZ48_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2260208.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            9406..9750
                     /locus_tag="PWZ48_000529"
     CDS             9406..9750
                     /locus_tag="PWZ48_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HDL2260209.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            10021..11307
                     /gene="efmA"
                     /locus_tag="PWZ48_000530"
     CDS             10021..11307
                     /gene="efmA"
                     /locus_tag="PWZ48_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HDL2260210.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(11445..12044)
                     /locus_tag="PWZ48_000531"
     CDS             complement(11445..12044)
                     /locus_tag="PWZ48_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HDL2260211.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            12448..13569
                     /gene="mnmA"
                     /locus_tag="PWZ48_000532"
     CDS             12448..13569
                     /gene="mnmA"
                     /locus_tag="PWZ48_000532"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDL2260212.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            13815..15236
                     /gene="arcD"
                     /locus_tag="PWZ48_000533"
     CDS             13815..15236
                     /gene="arcD"
                     /locus_tag="PWZ48_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HDL2260213.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            15250..16368
                     /locus_tag="PWZ48_000534"
     CDS             15250..16368
                     /locus_tag="PWZ48_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HDL2260214.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(16428..17720)
                     /locus_tag="PWZ48_000535"
     CDS             complement(16428..17720)
                     /locus_tag="PWZ48_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2260215.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            17852..18430
                     /locus_tag="PWZ48_000536"
     CDS             17852..18430
                     /locus_tag="PWZ48_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2260216.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            18559..18936
                     /locus_tag="PWZ48_000537"
     CDS             18559..18936
                     /locus_tag="PWZ48_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HDL2260217.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            18869..20941
                     /locus_tag="PWZ48_000538"
     CDS             18869..20941
                     /locus_tag="PWZ48_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2260218.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(21152..22099)
                     /locus_tag="PWZ48_000539"
     CDS             complement(21152..22099)
                     /locus_tag="PWZ48_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2260219.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            22345..22809
                     /locus_tag="PWZ48_000540"
     CDS             22345..22809
                     /locus_tag="PWZ48_000540"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDL2260220.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            complement(22871..23914)
                     /locus_tag="PWZ48_000541"
     CDS             complement(22871..23914)
                     /locus_tag="PWZ48_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HDL2260221.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(24291..25256)
                     /locus_tag="PWZ48_000542"
     CDS             complement(24291..25256)
                     /locus_tag="PWZ48_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HDL2260222.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFTLSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25490..26335
                     /locus_tag="PWZ48_000543"
     CDS             25490..26335
                     /locus_tag="PWZ48_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2260223.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            26526..27713
                     /locus_tag="PWZ48_000544"
     CDS             26526..27713
                     /locus_tag="PWZ48_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2260224.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            27719..28075
                     /locus_tag="PWZ48_000545"
     CDS             27719..28075
                     /locus_tag="PWZ48_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HDL2260225.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            28077..28415
                     /locus_tag="PWZ48_000546"
     CDS             28077..28415
                     /locus_tag="PWZ48_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HDL2260226.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     misc_binding    28491..28747
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            28810..29844
                     /locus_tag="PWZ48_000547"
     CDS             28810..29844
                     /locus_tag="PWZ48_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260227.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     gene            29837..30523
                     /locus_tag="PWZ48_000548"
     CDS             29837..30523
                     /locus_tag="PWZ48_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260228.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            30547..31395
                     /locus_tag="PWZ48_000549"
     CDS             30547..31395
                     /locus_tag="PWZ48_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HDL2260229.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            31592..32365
                     /gene="sufC"
                     /locus_tag="PWZ48_000550"
     CDS             31592..32365
                     /gene="sufC"
                     /locus_tag="PWZ48_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDL2260230.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            32378..33664
                     /gene="sufD"
                     /locus_tag="PWZ48_000551"
     CDS             32378..33664
                     /gene="sufD"
                     /locus_tag="PWZ48_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDL2260231.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            33661..34896
                     /locus_tag="PWZ48_000552"
     CDS             33661..34896
                     /locus_tag="PWZ48_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2260232.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            34883..35353
                     /locus_tag="PWZ48_000553"
     CDS             34883..35353
                     /locus_tag="PWZ48_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HDL2260233.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            35358..36749
                     /gene="sufB"
                     /locus_tag="PWZ48_000554"
     CDS             35358..36749
                     /gene="sufB"
                     /locus_tag="PWZ48_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDL2260234.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(36845..37987)
                     /locus_tag="PWZ48_000555"
     CDS             complement(36845..37987)
                     /locus_tag="PWZ48_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2260235.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWIAHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNDLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGNVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(38026..38760)
                     /locus_tag="PWZ48_000556"
     CDS             complement(38026..38760)
                     /locus_tag="PWZ48_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2260236.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLRNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            38992..39267
                     /locus_tag="PWZ48_000557"
     CDS             38992..39267
                     /locus_tag="PWZ48_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260237.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMEQYKG"
     gene            39365..41362
                     /locus_tag="PWZ48_000558"
     CDS             39365..41362
                     /locus_tag="PWZ48_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260238.1"
                     /translation="METPMINIPINVDKERLLEKPVIEDEKKRIRKRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNKSEEFLETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKALSINLVHNSAFAIQKSIRYSENLEKFKIVFLLKEAITDYSKMVAVYEYLKKI
                     IPGCDDNVNASNRMFFGGYKSDAVIMINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMDKLLKSSKNL
                     RCLFHDDHNPSASIFTSKTGHSIFYCHSSNCSISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMEDKLFRTIRNYLEEMRHVYDILLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGVVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(41438..41773)
                     /locus_tag="PWZ48_000559"
     CDS             complement(41438..41773)
                     /locus_tag="PWZ48_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2260239.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            41901..42116
                     /locus_tag="PWZ48_000560"
     CDS             41901..42116
                     /locus_tag="PWZ48_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2260240.1"
                     /translation="MKKVSNLSRYRKEKGFSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            42153..42515
                     /locus_tag="PWZ48_000561"
     CDS             42153..42515
                     /locus_tag="PWZ48_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDL2260241.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGADAVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            42561..42887
                     /locus_tag="PWZ48_000562"
     CDS             42561..42887
                     /locus_tag="PWZ48_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260242.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYSLNRTCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYT
                     EVLMLN"
     gene            43374..43511
                     /locus_tag="PWZ48_000563"
     CDS             43374..43511
                     /locus_tag="PWZ48_000563"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260243.1"
                     /translation="MAHNERFKQEIIQELEKGVQHQKLISKTAQKFDISLAKEVKLHE
                     V"
     gene            43576..44358
                     /locus_tag="PWZ48_000564"
     CDS             43576..44358
                     /locus_tag="PWZ48_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260244.1"
                     /translation="MKNSSNSAEVNVSTSTSEAVQCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDGVKYSEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKKVSGENNETEAVTEQTMDERIDSSESNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKNVPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(44852..46141)
                     /gene="dcm"
                     /locus_tag="PWZ48_000565"
     CDS             complement(44852..46141)
                     /gene="dcm"
                     /locus_tag="PWZ48_000565"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HDL2260245.1"
                     /translation="MFANMSETLNVLELFAGVGGFRVGLEHSNADLFKTKWANQWEPS
                     RKSQDAFEVYDYRFPNSENINRNIEEISNEEFEQMDADIIVGGFPCQDYSVARSKKHE
                     MGIEGKKGVLFWQIIRATNHIKPKYLILENVDRLLKSPSKQRGRDFAIMLASFNDLGY
                     SVEWRVINAADYGRRQRRRRVFFFVYKNDTGFANKIDARFEQKILGEEEHRYDEYIFS
                     EGLFARQFPIIKTPYKGRQASYKLSNDIVEISDNFTGKVYNTGVMRHGRYFTIDTEPY
                     DKEKPTPLREIIQPEDEVAEKYYLTDESKIKKFEYLRGPKKIERVTAEGHTYTFSEGG
                     MSPYDDLDMPGRTMLTSEGSVNRSTHLLKIDGRYRLLTPIEAERLQDFPDDWTKYKKL
                     SSGEIVEVSDRMRLFFMGNALVTKIVERIGDELQQLL"
     gene            46352..47830
                     /locus_tag="PWZ48_000566"
     CDS             46352..47830
                     /locus_tag="PWZ48_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutH"
                     /protein_id="HDL2260246.1"
                     /translation="MSEYLTKEQVHNRAKEAVGKSMIELNNGIPLLNSKNKNSIGDIF
                     ESWFGKVKDSESRPDLEEAGVELKATPIKKLKNGQYSAKERLVLNIINYNEVAEETFE
                     ESHFLYKNNIMEIAFYEYLKDTPKDQWTIREAILYEMYKNPVDFEIIRQDWELIKKYI
                     LDGKAHELSESLTNYLAPCTKGTNAVKSLRTQPYSEQKAKQRAYSLKSGYMTSILRKY
                     VLGDEKIDSIVKNTFQLEENNLEDLVLEHFIPYVGQTVQSLCEKFTIKTSYQANYRIV
                     SSILGLSGTVTKGEPFPRVEEFEKASILVKTIYFDKSGNNKESMSFPSFNFIDLSKQV
                     WEKEDGEPSSDWHNFLLDTRFLFVVFKEEDEGVVFKGAKFFSMPEEDIEGPVRKVWED
                     TVSKLREGVELEAIPDKSTKDGWKIKNNFIKKTDKMICHVRPHASQRDYSVDGRYADK
                     LPVPAKWINKPDNDNYSDQWMTKQCFWINNDYIKEQVQDLIS"
     gene            complement(47844..48275)
                     /locus_tag="PWZ48_000567"
     CDS             complement(47844..48275)
                     /locus_tag="PWZ48_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HDL2260247.1"
                     /translation="MKHRQLSTTPEVSKRMANVRLKGGKAESLLAKELWHQGVRYRKN
                     YKKLPGSPDIAVTKYKLAIFVDGEFWHGHDWDNRKQKLKRNRDYWIEKIEENMRRDER
                     NNQSLIKLGWVPIHFWEKDILKNLNYCTSVILDYIAQKKEE"
     gene            complement(49847..50512)
                     /locus_tag="PWZ48_000568"
     CDS             complement(49847..50512)
                     /locus_tag="PWZ48_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260248.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            50692..52674
                     /locus_tag="PWZ48_000569"
     CDS             50692..52674
                     /locus_tag="PWZ48_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HDL2260249.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            52678..53316
                     /locus_tag="PWZ48_000570"
     CDS             52678..53316
                     /locus_tag="PWZ48_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260250.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            53344..53772
                     /locus_tag="PWZ48_000571"
     CDS             53344..53772
                     /locus_tag="PWZ48_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260251.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            54229..55140
                     /locus_tag="PWZ48_000572"
     CDS             54229..55140
                     /locus_tag="PWZ48_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2260252.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            55357..55794
                     /locus_tag="PWZ48_000573"
     CDS             55357..55794
                     /locus_tag="PWZ48_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260253.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            55889..56773
                     /locus_tag="PWZ48_000574"
     CDS             55889..56773
                     /locus_tag="PWZ48_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2260254.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            56997..60164
                     /locus_tag="PWZ48_000575"
     CDS             56997..60164
                     /locus_tag="PWZ48_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260255.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            60184..60729
                     /locus_tag="PWZ48_000576"
     CDS             60184..60729
                     /locus_tag="PWZ48_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 domain-containing protein"
                     /protein_id="HDL2260256.1"
                     /translation="MNRIWNKSHRNNCSLLMILLLGFILWQTPVHASEFNFAVDFELP
                     ANQIDKNKTYINVRMEPGKTQEIPYTVFNRTDKSVTVETLIHSATTNSNGVIEYGESK
                     SKFDETLSYDMKNLAKTVGEITVPPQSAVTETLSLKAPEKELAGVVAGGITFQEKKEN
                     VSNKSTVGVVNQFSQVKAILI"
     gene            60757..61212
                     /locus_tag="PWZ48_000577"
     CDS             60757..61212
                     /locus_tag="PWZ48_000577"
                     /inference="COORDINATES: protein motif:HMM:NF023225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3324 domain-containing protein"
                     /protein_id="HDL2260257.1"
                     /translation="MKLKKAYADQSDGRNVIKANLQNPNASYMFGLTTSVEIYKKETK
                     EPYLSIKKEKIDVAPNTNFDLLVPLEGKKLAPGHYQAVVKGAWKEKRWNLHTDFEITD
                     DAADQLNEKDIDLKNQPETFDWRWLILVGVILIGLFAGLFILKERRKKK"
     gene            61209..61967
                     /locus_tag="PWZ48_000578"
     CDS             61209..61967
                     /locus_tag="PWZ48_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2260258.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(62053..62793)
                     /locus_tag="PWZ48_000579"
     CDS             complement(62053..62793)
                     /locus_tag="PWZ48_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2260259.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            62854..64107
                     /locus_tag="PWZ48_000580"
     CDS             62854..64107
                     /locus_tag="PWZ48_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2260260.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            64429..64656
                     /locus_tag="PWZ48_000581"
     CDS             64429..64656
                     /locus_tag="PWZ48_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2260261.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            64779..65105
                     /locus_tag="PWZ48_000582"
     CDS             64779..65105
                     /locus_tag="PWZ48_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2260262.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            65173..65886
                     /locus_tag="PWZ48_000583"
     CDS             65173..65886
                     /locus_tag="PWZ48_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2260263.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            66196..67047
                     /locus_tag="PWZ48_000584"
     CDS             66196..67047
                     /locus_tag="PWZ48_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2260264.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            67168..69033
                     /locus_tag="PWZ48_000585"
     CDS             67168..69033
                     /locus_tag="PWZ48_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2260265.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            69081..70490
                     /locus_tag="PWZ48_000586"
     CDS             69081..70490
                     /locus_tag="PWZ48_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HDL2260266.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            70598..71299
                     /locus_tag="PWZ48_000587"
     CDS             70598..71299
                     /locus_tag="PWZ48_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2260267.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            71458..71937
                     /locus_tag="PWZ48_000588"
                     /pseudo
     CDS             71458..71937
                     /locus_tag="PWZ48_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            72785..73360
                     /locus_tag="PWZ48_000589"
     CDS             72785..73360
                     /locus_tag="PWZ48_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HDL2260268.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            73360..73800
                     /locus_tag="PWZ48_000590"
     CDS             73360..73800
                     /locus_tag="PWZ48_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2260269.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            73816..74517
                     /locus_tag="PWZ48_000591"
     CDS             73816..74517
                     /locus_tag="PWZ48_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2260270.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
BASE COUNT        24745 a        12794 c        15380 g        21601 t
ORIGIN      
        1 tttttccgtt aataaatctc tttattatca acaacttaag ttatattttt tctttttata
       61 aaatacactt attataattt tgttttttat tgacagtaaa aataaattag gtaatacttt
      121 aagtaaacgc tttctaattt gttacgggag gaatacactt gttaaaaaaa cgtataaaat
      181 acagtgcgct aatgatgaat ctccttatgc tcagtacccc ggttttaatt tctgtcgttg
      241 aagttgctgc taacgaacaa cagtctgtga acgaagaaaa tgcacacgtg aacattgaag
      301 ggatgtcaac aatggaggaa ataccaagaa ctagtgaaga tatggatacg gaagaaggaa
      361 atgatcctat gatgaaagaa tcagaaaatg aaaagacaaa aaagcaatct gaagaagcaa
      421 atgaagagtg gaaaggggtt gttttcggag aaagcacaag tgctagtagg tatgccattg
      481 aaccattaga agacggcgtg cggctctctt ccacgaataa cggcggaaaa ttccagtcaa
      541 gcggatcaga tggaatgacc tattattatc aagcaatccc caaagacatc aactttagga
      601 tgcgggcgac gatcgaaata gaatcttgga cctatacgaa tgcccaagaa ggatttgctt
      661 taatggtaag agacgcagta ccaaaaaatc atttgtttgg gcaggcattt tattcaaatt
      721 catttgccat actaggcagt cggatcgaat atgtctggga ctcggataga caagaagtgg
      781 tcaacacgag cggcagcaaa tatacgatgc gacttggatt ggggacacgg gcaatcacag
      841 gaatatcatc tgaagatccg caagctgcgc cagctcctgg gagtgtctca gtggaaacaa
      901 cacctttaga gacttcagca gggttcttgg aaaaagaaac aggttcttat aatatattcg
      961 gaaacggtca tgggaatctg tttccggcca cgaactcgat tactaaactg gacgtagaga
     1021 tgaaaaaaac gaatactggt ttagaggcat attattacga tcctgaaaca ggagaagaaa
     1081 tggcttctga catcatgtat gactgggaaa aattatacgc ttcagatgaa tcggttattt
     1141 atgcaggatt tgctgcagca aggaatatga cgatcaaggt agaaggaatc gaagttgctt
     1201 actcagatcc tgcgacagat cctcctgggc aagaaagacc tactcgcttg attgatccga
     1261 tttacactgt gacctctagt aatcattcag gaaaccaaga tcatactatg tcctttagag
     1321 caaatgctga cggattattg acaattaaaa ataaagcaac aggagaggtg cttaagaacg
     1381 caaaaaactt ggctatcact gcgaatcggg aatttgatta tcagacaaaa ctgatggaag
     1441 gaacgaacga atttactttc agttttacgc cagataaaaa ctatccgcca gaagaatacg
     1501 tagagatgac tagttatgaa acaaaagaga ttcttcatac agtggactac cgtaaaccta
     1561 aatattcgac agtttatgta acaccagaag ggtcagatgc aggaaacgga agcagacaaa
     1621 atccattatc tctgacacaa gcattgagat acgcatatgc tgggcagaca atcgtcatgg
     1681 cgcctggaac ttattccttc gaaagaggat tgacgattcc aaaaggcgtt aacgggacaa
     1741 gagaaaaccc gattcagttg attgcagaga aaaatccaga aaacaagcgt cctgtacttg
     1801 attttaaagg aactggaaat gggatgacac tgttcagtca ctactggggt ttacgagggt
     1861 ttgacattac aaatagtgca gctatgcaaa aaggattaca gatttctgga aatcataatt
     1921 tgatcgaaga gattcatgct tatcggaatg gaaatacagg aatccaaatc agcggtagct
     1981 caaatgatcc tttcgaagca tggcccgcgc ataacttagt caagaactgt acatcttttg
     2041 aaaatgccga tccaggattt gaagatgccg atggatttgc ggccaaactg acaataggag
     2101 aaggaaatgt atttgacggt tgtatcgcgt atcataatgc agatgacggt tgggaccttt
     2161 ttgctaaaaa tgaaacaggt tcgatcggaa aagtaatcat caaaaactca gttgcttatc
     2221 gaaacggctg ggtaccagga attgagggag aaggtaacgg taatggattc aaaatgggcg
     2281 gttcttcttt aacgggccct catgaattga tcaatagttt gtctttcgaa aatcttgcga
     2341 aaggaatcga ttccaatagc ggaacagata ttatcgtcaa tagcagtaca agttttaata
     2401 acggttcata taatgtggct ctttatacct cttcagcagg aaacacagac taccatgctt
     2461 ctggtatctt atctttccga acagagaatc ttgagatgag agagcagatc cgcccattga
     2521 atcaaaacga agatcaaatc taccactcgt tgaactacta ttggaatgaa cgagagaaac
     2581 aatcagaaaa tactcttgga cataccatat caaaagattg gatcgagtct ctaaatacat
     2641 gttcgaaaga aggtcagcag cctgttacgc gctatgaaaa tggaagtatc catgttcatg
     2701 gactgatgca gattaaaccg gggaatcgtc aaacagaaaa ggaaagagtg gatggtcttc
     2761 cattatctgt aggtgctatt tttgacggag aaacctcacc gtcttcggaa tatccagagt
     2821 atgcaatcgt acaagaacca aatcagacaa aaccaaatga acaaaataat ccgattatta
     2881 tacaagaagc aaatggtgtg gcggagaatg cagaaggaaa tatcattgaa ctgaagcctt
     2941 ttacagtttt tgtgtcaact ttcacaccaa atcaaggaac gcctgtcttt gaagatatgc
     3001 gttatttccc agaaaagatc caaatcgttg aaacacagtc aagtgctcaa ttttctaatc
     3061 ataccgctat tttttcagca ccttcaaaag gagaatatac gctagaagtc acttatcgtt
     3121 cggaaatatt tgatggggaa aaatggtctg ctcatacaga atctgttacc cagcaaaaag
     3181 cgatcactgt gcgagaaact gccgatgtgc ccccgggaaa tgacaaacca gaaagagaaa
     3241 atccaggaaa tgaatcacca aatggaaaac caaatggaaa accttctcat ccggatagga
     3301 cagcaaaaga aagtgaaaaa aatggcactc aaggactccc aaaaacggga gaatttaata
     3361 acccgttgct agcattagct ggaggaattt tgttaatcgg tgtagttgtt tatgtcatga
     3421 aacagcgaaa aaaataagtg gaatttctcc ttttatgatg taaaacaatt gactgaggaa
     3481 agtaaaaata gaaggaaatg agaccaatag atgtctcact tccttctatc tttttataga
     3541 tgaggttctc cttgctgctt ttgattggct gttcttatta cttgatgcag gacagctttt
     3601 tcacaatctc tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaacaaac
     3661 ggcaaaatat gaggaactat ttttccgttt ttttctctaa tactcaggtc aggacgctga
     3721 tttcacaacc tctcgataaa cggtgttcaa aagccagttt ctcggtagta agtcaaattt
     3781 atgcaaaaat gtggaaagcc attttcacaa aatttttctt actacttgaa actaagcagc
     3841 tttttcacaa cctctaatac atggtattcc agaagtaact tcttcgaaaa ataagccgaa
     3901 atgtcacaaa aatttgagaa gcgattttcc caaattcctt cttatttctt gaagctgacc
     3961 acttctggca caacctctta ccagattcgc actctttttt ctggcgctag atacatagcg
     4021 tcttgttccg tgataccaaa tgtttcatag aattcgtcaa ggttttttgg ttgcacgttt
     4081 gctcggagtt tagcaggggc atggacatcg atttgcagta aaagttgttg gtattctttt
     4141 tttgcttttg tacgccaaat cgttgcccaa ttgacaaaga attcctcgat agaaagatct
     4201 gattccgttt ttgctgcttc caatgcacag cttagaccgc ctgcatcagc aatattttcc
     4261 gaaacggtta attttccgtt tacttttccg tcagcaaaag gcagaccatc gaattcttcg
     4321 atcattgctt gtgctttgtt ttgaaattgt gtaagatctt cttctgtcca ccagttgttc
     4381 aagttgccat attcgtcaaa taaagcgccg ttattatcaa atgcatggga aatctcatga
     4441 gcgataacag ctccgattcc accataatta gcagagcttg attgttctag actgtaaaat
     4501 ggtgcttgaa ggatcgctgc cggaaagacg atcacattcc gaaatggatg ataataagca
     4561 ttgaccgtgt cagcactcat ttcccattct gaacgatcca ctggtttgtt ccatttgctg
     4621 aaacgatctt ttaatgtgag tttgctgaaa acaagagtat ttgaaagcaa agtaccaccc
     4681 tgatctgcag gtactgtttt gaactggtcg aataatgctg ggatcttatc ggggtagcct
     4741 acttggatac ctaagttatt taattttatg acggcttttt cttttgttgc atccccaagc
     4801 cagtcatttt cttctagacg ttttttataa acggcgatca tttttttcac catttgttct
     4861 acgtcttttt tagcagcttc gccaaaatat ttttttccgt agtaatcgcc aactacttga
     4921 tcgaattgtc cagaggcaag gtaatacgct gcttttttct gaggcattgc ttcgtctgtt
     4981 ccagacaata cgcgagaata gattccgctg acttggcgga attcttctga tagataacca
     5041 ctaagagaac gtactgtttt aatgatcatc cagcttttga ataacggaaa atgagcttct
     5101 gtcaggatgt cattcaaatg atcgaagtac actggatcag ttacgatcac tttatctgga
     5161 gttgtcccaa tcaaacccgt gacaagtttt ttcaagtcca gtttatctgt gtatttaata
     5221 aatgtatcaa aagattgcgg attatacatt ttgctgtaat ctgcgttttc ttctgcactt
     5281 tttacatgag gagcaatcaa tttatcaaat tgcactgctt gttccgtgat tgcttctgcc
     5341 tgactttttt ctttgccggc aagctctaat aactgaacag ccatatcaaa gaaaacgcct
     5401 attagttgtg caccgttggg atggtctgat gcataatacg tcttatctgg aaggaataaa
     5461 gaaggcggat aagcaaagaa ggcgttcgtt tgcgcattct tcatatctgc atctacatcc
     5521 agattaaatg gcaatggtaa tgtatccaac agccattctg gaaattgctt attcaattcg
     5581 tcgaaagact gcaatgattc aactttttcg attataggtt gaagcggttg agcatgcaac
     5641 tcatttcttc ggtcataatc gtttgccagt tgataatatg caaggaaatg tttcattttt
     5701 tctgaagaca ctttttctgg atgggcaagc agatcgtctg tttctcccat caaaagttgg
     5761 tcgatcccgt caactaaatc ttggaaaccg cctgtagctg gtttatctgc agggattttt
     5821 gctgtttttt gccattcttc attgactgct tcatagaaat cttgtttcaa tcgttctttg
     5881 ttcattcatc gatcgctcct tttttgtttc gtgtgcccat tatcttacaa aatgaaggaa
     5941 ttaacaaata gaacataaaa aattgaaaat tcgaacaatc aaactgattt gtttttttca
     6001 atccttcact tataatgaaa aaaaggaggc gatcccaact gaatcaagta gtggataaac
     6061 ctttatattt caaagtagat aaaaaaatta aaaaattagc cagcactcag cagctgcagt
     6121 caagaaaaag taaattgctt tttttagctc tggtttttga agatcaaagt tatgtgatta
     6181 tcgatcaatc aggacatccg gtagaatatt ctccagcaaa atatacttat caagagggtc
     6241 tttcttgtaa aaaatggaag ctgatcaatg aagagccaat cgagttatct agatggatca
     6301 acagaaaaga agatattccc gttttgatcg aagaaaaaag gtcaggaaag gaactcgcaa
     6361 attgctgggt gggattgcca gaagaacgtt ttttgaggta taagaaatgg gctaccccct
     6421 caggctatct ttgtggtact tacgcggcag cagttctcct tgcctattat caagactatc
     6481 gtaaggggtg gatgcttcca aatgagattc gcaagaaaaa tacggctgat tcgttggttt
     6541 tgacgaaggc attgcgtagc cagatccaac cactaggact tcctacgatt ccttttcagg
     6601 ttagtacagg gatttctagt tttttaaaga agaatggcaa tcatgagcgt gcacgagcaa
     6661 ctcttctagg ttcatggcag cgagctacaa aacgtatacg tgaaggcaaa cctgtgatga
     6721 ttggtatttt gaaagtactt ggcagtacat atggcaatca ttgggtgaca gcgtacgctt
     6781 attttgaaac tgaaacagga gaacgttatt acaaagtaca cgacaactgg ggcgattatc
     6841 ataaagtcat tcctgccagt tggagtaacg gaacagtttc acttccttaa gaatttgctg
     6901 aaattaacag aaaatcttgg aaaaatatgt taaactatcg aagaatagag aagttttggg
     6961 gaggactaca tgagtaataa gtatcaggat gacacgttga agatttttag tttgaatggc
     7021 aaccgtccat tagctgaaaa aatcgcgaaa gtgttcggaa cagaattagg taaaagcgtc
     7081 gtgaagcaat ttagtgatgg cgagatttcc atcaatatcg aagaaagtat tcgaggggat
     7141 catgtgtata tcgttcaatc aacgaatgca cctgtcaatg attactatat ggaactactg
     7201 atcatgatcg atgcaatgaa gcgagcaagt gcgaaaacga tcaatgtcgt attgccttac
     7261 tatgggtatg ctaggcaaga ccgcacggcg aaacctcatg aaccgatcac tgcgaaattg
     7321 atcgctaact tgatcgaaga agcaggggca actcgagtac tgactttaga tctgcacacg
     7381 gttcaagtac aaggattctt tgatattcct gtagataatt tatttacgat gccgttgttt
     7441 gctcattatt accgtcaatt aggtctgacg ggagatgaca tcgtagttgt ttcgccaaaa
     7501 aacagcggtg ttcagcgtgc acgtagtctt tcagaatatt tgaacagcac attagccatc
     7561 gtagatcatg cagatgatgc ttcaactgag ggtggttatg tgatcggtga tgttcaagga
     7621 aagacatgta tcatggtaga tgatatcttg aacacaggag caacgcttgc acgagcagca
     7681 aatgtattaa aagaaaacgg cgcaaaagaa gtatatgcct gtgcgtcaca tggattattg
     7741 tccgatcatg cgaaaagtat tttagacaat gcaccaatca aagatatttg tatcactgat
     7801 tctgttttca cagaagaaaa tcgtcatcca gaaaacttaa caatcgttac atgttcagaa
     7861 ttaatgggtg aagcggtcaa acgcatccat gaaaatacac cgatgagtcc gttattccgt
     7921 ctggaagaaa aagaattcga ataaacaaaa aaaagaggga aagaagttgt gacagaagtg
     7981 agcagcttca aggagctgcg acaagacttt tgtcacagct ccttttttct atgcccatga
     8041 aacgtcctct agacaaaaat gagctcttct ttggtttgtt cgctatttta tgctagaatg
     8101 tgcctagcaa taatttggaa gggatgaatg aagtgaaaac tatttatctg gatcatgcgg
     8161 caactacccc catgcatcca caagtaattg aagcgatgac ggcaatcatg caagacacgt
     8221 ttggaaatcc ttcaagcata catggatttg gtcgccacgc gcatgaaaaa ttggaaacag
     8281 caagacaaat cattgcagac agtctgcaag caaaacctca cgagatcatc tttaacagtg
     8341 ggggaacaga aggggataat acagctatct tagaaacagc gttctcgcgt caaaaagaag
     8401 gcagacatct aatcactaca gctatcgaac atcctgctgt tcttaatacc atgaaatatt
     8461 tagagacaaa ggggtttgac gttacatatc tgcctgtaga tgaaaaaggg aatctgtcga
     8521 tcgaagaggt caaaaaagcg ttacgagaag atacgatctt ggtctctgtg atgtacggga
     8581 acaatgaaat cggaaatttg atgccaatca aagaaatcgg tgaactttta gcggatcatc
     8641 cagcttattt ccatacagat gcggtacaag cgtacggaaa ccaaacgatc catccagaag
     8701 aattaggaat cgatttatta agtatctctg ctcataaaat caatgggcca aaaggcgtcg
     8761 gtttcttata taaacgagac ggagtaaata tccctgcatt gcttttaggc ggggagcagg
     8821 aagaaaagcg gcgtgcaggt acagaaaatt tagcagggat ctggggtatg gcaaaagcag
     8881 ttgaactgct gtcacctgaa gaacgtgaag cacgtaacaa caaatataaa ggattccagc
     8941 aattgattct tgatacatta gaagcaaacg aaatccctta tcaagtaaat ggtgatttaa
     9001 cgaataaact ccctcatgtg ttgaatcttc gcttaattgg cgaagcaaat gatttgctac
     9061 tgatgaagct tgatcttaat ggggtcgcta tttctactgg ttcagcttgt acagcaggaa
     9121 atatcgagcc ttcacacgta ttagaagcaa tgtatggaga agatacaccg atactaaaag
     9181 aatccatccg tatcagcttt ggtttgggaa ataccgaaga cgacatccag tatttccttg
     9241 atcaattagt tcgtgctgtc aaaaaataac tagtgaagtc attcttatat gaacagatgt
     9301 ctgagagaac gccttcattt tttagggaac ttaactgaaa aacctagtaa aaatcaggat
     9361 aaacttttat aataaagaga aactaatttt atcgaggtga aaatcatggc atttgaaaca
     9421 acagcaacgg tactgggcgc accaattagc tatcgtttgc atcctgatgc aaaaagatat
     9481 acattgcgcg ataatggatt tacagaaaca aacggaggca attatcaatt gatccgacca
     9541 ttagatgcca cgccgcaaag caaagaagga ttcaaattaa aaatcactgt atcaaaagat
     9601 attcaaacat tgaaaatgtc gatcactaca gcaaatggat tgaaagcagt caatattttc
     9661 aaagatccaa aacagaaaat gagccaagat aaattttatt ttctgatgga tgggatgatt
     9721 agccgtgggt tattcgaaaa agtcgaatga tctgtggata taaaaagaca actagttttg
     9781 aaacggccta ttgttagatg actaactttg taaggacgac gcaaattagt tgtctttttt
     9841 atttttaaat ttaacacatg ttgttatttt taatattttg ctgatttatt gtttctaagt
     9901 aaagaaattt aaattgaaac gattgttatt tagggaaaaa ggtatttttt gaaaaaagaa
     9961 tcccgtataa tgattgttgt gcaaacgcta tacaaaatga aaaagaaaag agggatgttt
    10021 atggaaaatg aacagtcggt cgttttaacg aattggaaga gaaattattt gttcttttta
    10081 tcgggacagt ttttgtcagg aatcactagt atggtcgtcc aatatgcgat catctggtat
    10141 ttgacaagag aaaccggttc ggcgacaatt ttgagttttg ctactttact aggaatgatt
    10201 cccatggttt tactaagccc atttgtagga ccgctagtgg atcgctggga taaaaaagct
    10261 cttttgattg tcacggatat tattgtagcg attttcgcgc ttattttagc agtagtcgga
    10321 acgatttcag aatccttccc gatttggcta gtttttgtgt ctttgtttat gcgttcggtc
    10381 gcacaaacat tccaaatgcc aacgatccag tcgattatgc cgacaatcgt tccatcttct
    10441 cacataacaa ggacgaatgg acagttagga atggtccaat cagcgaattt catcattgcg
    10501 ccagctctag gtgcggcttt gttttcagtc gttcctgtca attaccttat tttattagat
    10561 gtattggggg cagtttttgg tgtcggctta ttgatttttg tgaagatccc aaaagtttct
    10621 cctgaaatat tggaagttcc gcttactatt tttaaagatg caaaatttgg gctgcagcaa
    10681 ttgatggata ataaagggtt atggtacata acgatcaatg gtgcatttgt gatgctgttg
    10741 ttcatgccag caatcagtct gtatccattg atgacgctag attactttgg tggttccgtt
    10801 ggacaggcgg gagcagtgga agtagtctac gcagtcggaa tgctcttagg cggtgcactg
    10861 atcagtttta taggaacatg gaaagaccga atgaaaccta ttattatagc ctacatcatt
    10921 atgggcctga cgatcggtgc aagcggattg gttccaaacg atagtcaagg gttcttgtac
    10981 tttcttatct tgaatgcagg agcaggttgt gcaacgccat atttcaatac actgctgatg
    11041 gcaatgatcc agcagagtta tgaatcgaat gttttaggtc gtgtcttagg gaacttcaac
    11101 tctttgatga acctggcagg tccaattgga ttattatttg caggaccatt agctgatcgt
    11161 ttaggggtag aaaaaatgtt cttgttttcc ggaatcggga ttttattatg cggaatcgtt
    11221 ttatttttaa cgtcagctgc ccgaaattat gataaagaat tacagaaaaa actggtaaaa
    11281 gaacaccatg agcaaaaaga cgaatagtat ggaatggagt tgtgacagaa gtcttgttac
    11341 aactccattt ctgatcctaa tgttcctgtg gcaataagcc tactcaaatg aaaaatatga
    11401 gtaattatct ttatttgttt aatacttgat atagggcagt tttttcacga cctatcgtac
    11461 ttcctctttg cttcatagat ttctttgatt tgttgctcag ataacggagt gccaaatggt
    11521 agtttttcct ttaagagttc tctttccggt gcgtctacgc ctacattgac atcgttctcg
    11581 ataggtacgc tgtaatgatg gatatgtcca tgaagattgc ggatagtgtc gttaggcccg
    11641 agcaacatcg gataatgagt caagtaatat tgttgatggt cgaatttaac gatggcacct
    11701 acatcataaa aataaaattt gggacttccg tcagtaagcc taggatcatt tttttctaaa
    11761 taacggaaaa aagcgcgact gtcatggttc cctttgatca agtggattgt tccttgcagt
    11821 tgagtgacca gttccaaaat ttctgcattt gctttttcat attgcggatg catagcaagg
    11881 tcgcctagat gataaacatg gtcggtttct tttattactg cattccagct agtgatcaac
    11941 tgttggttca tttcatccac atttgcaaag agccgcggag caaagtcgtt ttttcctaat
    12001 aagcgttcat ggaaaaagtg catatcactg ataaagtaat tcatgtatct tttgcctccc
    12061 ttttttctta gagtaaacaa aatcacttat ctgagcaact cataacatct aaaaagaaaa
    12121 ggttgtaaat gagacgtgca gctttaagcg ataagaagca attttgaaaa atagtttttc
    12181 acaatttata aaattattcc ttattgccga aaggctggcc ctttttatga gtatgccaat
    12241 tttttatatc gaatgctcaa aagatgagtg attttttggt tgactatcat gtttgtgtac
    12301 tgaataggtc cagtagaaca gtatttttac gacttgataa ttaagggatg aaattttcag
    12361 aaatatgacg tataatgtaa aatactgaaa agttgcgacc ttcaactcgt agatgtttca
    12421 gaatctaatt gaaatgatgg tgatatcatg acagacaaca gcaaaacacg cgttgtcgta
    12481 gggatgagcg gcggtgtcga ctcatctgta acagcacttt tgttaaagga acaaggatac
    12541 gacgtgatcg gaatattcat gaaaaattgg gatgacacgg acgaaaacgg tgtatgcacg
    12601 gccacggaag attataagga tgtagcgaaa gtagctgctc aaatcgggat tccttattac
    12661 tctgtgaatt ttgaaaaaga atattgggat cgtgtatttg aatatttcct agccgaatac
    12721 cgcgcaggac gtacacctaa cccagacgtg atgtgcaaca aagaaatcaa attcaaagca
    12781 ttcttggatt atgcaatgga tcttggcgcc gattatgtag ctactggaca ttatgcgcaa
    12841 gtcactcgcg atgaaaacgg cgtggtccac atgcttcgtg gagtagacaa taataaagat
    12901 caaacttact tccttagcca attatcacaa gaacagcttt caaaaacgat gttccctcta
    12961 ggcggcatgg aaaaatcaga agtacgggca attgctgaac gcgcaggcct tgcgactgct
    13021 aagaaaaaag attcaacggg aatctgcttt attggtgaaa agaacttcaa acaatttttg
    13081 agcaactatc tgccagcaaa aaaaggaaat atggtcactc ttgatggtga agtcaaagga
    13141 caacatgctg gcttgatgta ttatacgatt ggtcaacgtc aaggactagg gatcggtggc
    13201 ggaggagatt cgcaagaacc ctggtttgtt gtaggaaaag atctagcgac aaacacatta
    13261 tatgtggggc aagggttcca tcatcctgca ttgtatgcca caagcttgga tgcaagtgaa
    13321 atccacttta cgacgaatga accaatgcca aaagaattca agtgtacagc gaaattccgt
    13381 tatcgtcagc aagacgttcc tgtgacagtt cgcttattgg atgataatcg tgcagaagtc
    13441 gtattcgacg aacctgtacg tgcaatcact cctggacaag cagttgtatt ttatgatgga
    13501 atggaatgtc tgggtggtgg tttgatcgac cacgcatatc aagaaacaaa agttttacaa
    13561 tacgtttaag cgatattaaa tgaatcaaaa cagctatggc taacaaaaga aacacctttt
    13621 cttttggata gcatagctgt ttttttgcaa taaaattaat ttttattcat tttatacttg
    13681 aaatacttgt gtaaaaagtt cataatatcc aataatatga ataaagttgc tttttgttaa
    13741 gcaagagcag ttgcaaaata gaagggaatc ttttgcaagt gatttgttca attttataag
    13801 tgagggtgat ggaaatggaa aagaaatcga cgggtattag caaatgggga ctggttgctc
    13861 tggttgtcag ttcgtcaatc ggaagcggtg tgtttgggat cacaagtgat ctggctagtt
    13921 ctgcagctcc agggccagcg atcctttcat ggataatcgt tgggattggt attttagctt
    13981 tggtattatc gctgaatcat ttaggagaaa agcgtccaga tctagatggt ggtattttcg
    14041 gttacgcgga agcttcattt ggtaaattag gtggcttcat cagtggttgg ggttactggt
    14101 tgtctgcgtg gctgggaaat gttgcttttg caacgatgct gatgagcgcc atgggagaat
    14161 ttttcccgat attcaaaggt ggacaaaatg ttccttctat tttatttgca agtattttta
    14221 tttggggtct gactttatta gtaaacaatg gagttgaagg agcgagcgtc atcaatacaa
    14281 tcgtgacgat ctgtaaatta gtgccattat tcctattttt aatatttgta tttatcgcat
    14341 ttaaagtgaa tatattcact gctgattttt ggggtaacgt atctgataat ctagtgaatg
    14401 gaacagggca gcaaggaacg atctggttgc agattaaagg ttgtttgatg gttatgatgt
    14461 gggtctttgt agggatcgaa ggcgcaagtg ttttagccaa ccgcgcgaag aaacgttcag
    14521 aagcccagca agcatcgatc attggtctgc tttgtttatt gtttatctat atccttgctt
    14581 cgatcttgcc atacggcgtc ttatctcaag aagagttagc aactatttca caacctgcta
    14641 tggcaaatat tttaaaagga atcgtgggaa attggggagc agcgctgatc aatatcggct
    14701 tgattatttc gatcattggt tcttggttgt cttggacgat gctcccagca gaaacaacga
    14761 tgttgatggc tcgagacggt ctgttgccga aaggttgggg aaaaacgaat aaaaaaaatg
    14821 ccccaactta ttcgttagtt gttactgcaa tcttgactaa tctattctta ttgactttct
    14881 tagttactga ttatgcctat caatttgcct attctttatg tacagcggca attttgattt
    14941 gttatctact tgtcgggatc tatcaagtcc agttttcttt aaaagaagga atcaaaaaac
    15001 aagtagtgat cggtgggatt gctgtagttt ttgaattgat gggcatcatt ttagcaggat
    15061 tttcttatgt ccttttatgt agtattgcct atcttcctgg tttttgcttc tactggatcg
    15121 cttgtcgtga aaccaaacat caaattacgg gaaaagaaaa agtaatgatt gggctgattt
    15181 gtgtggccgc actgctttca atcgggttgc ttgctgtagg ctggatcaag atataagggg
    15241 gaaagaaaca tgaaaatcgc aaatttcggt gtagaagaat ggctaaataa atgggaaaag
    15301 aaagcggtat atgatattgc acaaagctcg atcgaagcat tgacattaga cgaactcgtt
    15361 ggcttagatg ggactagcgt aagtaaattt ttcgaaaaac gcaaaaacga accattggac
    15421 tatggctgga tcgaaggatc agatgttttc aaacaagaag tcgcttctct ttatcaaacc
    15481 gtcgatccgg aaaacatctt acaaacgaat ggagcgactg gtgctaatct tttagctcta
    15541 tacgcgttag tggaagcagg agaccatgtt gtttcgatgc ttcctactta tcagcaatta
    15601 tatgacatcc ccaaatcttt aggtgccgct gttgactttg ttcatttgaa ggaagaagaa
    15661 gattggcagt ttgatttaga acagctagaa gccttagtca aacctgaaac aaaaatcatc
    15721 tgtttgaata gtgcaaacaa tcctacaggc acattgctag atcggaaaac tttagaaaaa
    15781 atcgcagaaa ttgctcgtac agtagatgca tatgtattga tagatgaagt atatgcgcct
    15841 ttaacggata aaggtgaatt cttgtcgatc gtagatgtct atgaaaaagg tatagcaacc
    15901 aattccctat ccaaaaccta ctccattcca ggtattcgta ttggctggac agctactgga
    15961 cctgagttgg cagaagtttt tcgaaagtat cgagactaca cgatgatttg cggcggagtc
    16021 ctgtcagatg atcttgctgt acatgcattg aagaataaag aaaaaatatt ggcgcgaaac
    16081 aaaaagatca ttacagagaa ccttgtgatt ttaaaacaat gggtagccaa tgaaccgaaa
    16141 gttgaacttg tcgctcccaa ttacgtctct acttctttca ttaaattagc catcgaagaa
    16201 gatgaccaga cattttgtat caatttatta gaagaaacag gagtactatt agtaccagga
    16261 agtgcctttg atttaccgaa gcatgcccga ctaggttact gttgtaaaaa agaaaccttg
    16321 gaaaagggat tattcctctt atctgagttc ttgaaaaaac aggcctaaac aaaaacactg
    16381 caaggacagt ctgtgacaac tgttcaagca gtgtttttca tttacgctta ttgtctatga
    16441 gaaggagagt gccctgaagc tatggagact cctgaagctt tttcttctgc agtttcttgc
    16501 tgatggatct cttgtgtatt gtgtcgtaca agcaagaagt tgaccaaaac aagaagagaa
    16561 gtcgaaagaa agacgccgcg ataaccaaat gcactggaaa cactggagcc gatcataggc
    16621 cctactacgt taccagttgc ttggaaagat tggttataac tgaagatccg tccagcagct
    16681 tgatgaggcg aatattttgt gatcaatgct tggacagcag ggagcagaca agcatcagag
    16741 ataccaatca aaaaacgtaa ggcagctaat tgccaaacat tcgtgacgaa tgccattggg
    16801 atataaacca gaatcgcaaa acccaaacca atcgcaagta ttctttcact cccaatcttg
    16861 tcacctaagc gaccaaatct tggagcggct atcaaagtgg caatcccagg tatcgaagca
    16921 atgattccgc tgatcaacgt aacattccca tgtccatgca gcaattggcg aatgtacaga
    16981 ctgatgattg gactaatgga attgttggat gcttggatga tcatcgttgt gacgaacata
    17041 ccgatgacga cgtgtggata tttcaagtct ttgaaaatct gtttggcaga aaccatgttt
    17101 tcttttttca ctgggacaaa tttttcatgg acgaaaaaca gactcaacaa gaagacgaaa
    17161 aataagatga tccctgtaat gaaaaaggta gggcgatagc caaacgctga agcagagatc
    17221 ccgccaagaa gcggtccaag taatgttcct gtaacagaac cagttgctaa ggttccaaga
    17281 acttgaccac ttttttcttt tggcgtccca gttgcgacaa gggcagtggc attactgata
    17341 taaccagaaa aaatcccttg aagcagacgc aatgcgacta attgatacac actagtcacg
    17401 caacccatca aagaaatgac gattgccatt cctaatgaag cacgcaacag catcaatttt
    17461 cttccttttt ggtcggctaa ttttccccac caaggagaga taagcgtagt caccaagaaa
    17521 gtagatgaaa aagtcaatcc gctccaaaag ttcaattgtg acgtactata attacctaac
    17581 gtatctatgt aaagtgacat aaaaggcatg actaaactaa atccgatacc agccataaat
    17641 gttccaaacc ataagacaaa caagttttgt tcccaagttt ctcttttcct aaataatttt
    17701 tcctttattc ttgcagccaa ttagactcct tctttctgaa aattttcatc tacagctctt
    17761 tattataaac tacaatatgt agtttatcaa tgaaagtctc ttttttctct ttctttttcg
    17821 tggtaaactg aaagagtgaa ggatttattt tatgagagga aacatggaaa tgaaaaaaag
    17881 aaatctgtct caagagaaaa tcatcgactg ctttcgagaa ctagcagaag agatggatgt
    17941 tcagcaaata acctttcagc atttagccaa acgcttggat atcaaggctc cctcgctgta
    18001 taaccatttc aaaaatattc gtgatgtcaa aacagcgcta acagcgaaac ttctgcagga
    18061 acttaatgac cagctgcgac gggctcttgt gggaaaaagt ggtcaggaag cactgcagat
    18121 ctatgcagaa acttatcgat cctttgcttt ttcaaatcaa gcagtgtatg agttactgat
    18181 cagtgtaccg cataccaatg aagctgtttt attagatgga atccatgaaa cgaatcagat
    18241 cattttgcag atatttgagg cttttcaatt aacgaaagaa gaaaaagttc atcgaagcag
    18301 ggaacttcgt agtatgatcc acggttatct ttccttacgt tttttaggat actttacaaa
    18361 agaaccaaca atcgatccag aagacagcta tttttggatg atcaataact ttatcgctac
    18421 tttgccttaa acaatcagat tcatttgtaa caattttaag atgttcatta aatgaccctt
    18481 ttataagaga aaattcttgc aactttcttg aaaagtgact aagatgagtc tagaaaagct
    18541 gaaagaagga gcacgagtat gtatcaagta attaccatgt tcggggacaa tgagccttgg
    18601 tggttttttg aagactggga agaagatatt gaagaagaag aaacattcga gtctttcgaa
    18661 gaagcacgac aagcgtacga aaaacaatgg aacgaaatcc atcaaaatta tgaatatatc
    18721 aatgcaaaaa gtaactttct cagcgccttt tggaatgacg gagatgagcg ctggtgtgaa
    18781 gagtgtgacg atgatttaca gcaatacaag ggacttgcgt tgttgaaaaa tcatcagccc
    18841 atcacagtag aaagcagaaa ggaattttat gaaacaacta attctagcgg aaaaaccaag
    18901 cgttgcgaaa gacctaagca aggtgcttgg tgctaatcaa aagcacaaaa attattatga
    18961 aggacccaag gtgatcgtta cttgggccct tggtcattta ttaggattga aaatgccgga
    19021 agatctgaat aaggaatggc agagctggca aatggacacg ttgccaatga tccctaaaaa
    19081 actaggaatc aaaccacttc ctaaaacagg acatcaatta aaagcaatca agcagctggc
    19141 tcagcgaaaa gatgtctcag aagcagtcat tgcgacagat gccggaagag aaggggaact
    19201 tgttgctcgc tggatcttag agtatgtgca tttcaataaa ccagtgaagc ggttatggat
    19261 ctcttctcaa acagataaag caatcaaaac tggattcaag caactaagac ctgcgaaaga
    19321 atatgatgca ttgtatgaat ctgctcttgc ccgggcaaaa gcggactggc tggtaggatt
    19381 gaatgtgacc agagccttaa ctgtgaagta cgcggataat ttatcagcag gccgtgtgca
    19441 gacaccgaca ctagcattag tgagagacca agaaaagaaa atcgagcagt tccgtccaca
    19501 aacgtatttc acgatcacgt tgcaagctgg aaaagaacaa gccaaaatgc ttcaaaaaaa
    19561 tcaatttgct ttaaaaacac aagaagaagc acaaaaactt gttcaattga tcagtacaag
    19621 caaaggaact gtcacagaag tccaagaaaa attaaaaacg gaatctgcgc cattgcctta
    19681 tgatttaaca gagatccaaa gagaagccaa tcaacgctat ggctattctg caaagaaaac
    19741 attaagtctt gtacaaagtt tgtatgagac ccataagatc gttacttatc cgcgtacaga
    19801 cagcaaatat ctgacaaatg acatgaaaag tacgatgaaa gaacgattgc aagcagtcag
    19861 cgactttgct cctgaggtca agcagtattt gaaaaacgga gccatcgtcc aacagacaaa
    19921 agtattccaa gacagtaaag tgaccgatca ccatgcactt ttgccaactg aaaacagagc
    19981 acgttatgaa aaattaagta atgaagagcg aaaaatctat caaatgattg tctctcgttt
    20041 tttaggactg tttgctcaac cccataaagt cagccaaaca aaagttacgg ttgaatttgg
    20101 taaagaacga ttcatttttc ggcaaaatcg tgtcgtccaa gcaggatgga aaggcgaaac
    20161 tgaaactgag acggaaacag tcaaatggga aaaaggaatg agaatcaatc ccgatttcgc
    20221 catcaaaaaa gaactatctg caccgcctaa accattgtca gaagcaagct tgcttggtca
    20281 aatggaaaaa tacagtctgg gaaccccagc tacgcgtgca gagattatcg agaaactaat
    20341 caaatctgaa ctgatggaac gtacccctca aggactgcaa gtatctccta aaggtaagca
    20401 attattagta ttggtaaatc cctctttagt aacaccagat ttgacagaaa gctgggaaaa
    20461 agaccttgaa gctattgctg caggaaagaa acaagcagga accttcttga atggaatcga
    20521 aaaagaaaca aaacgcttgg tgaatgagat caaatcaagc aaacaagaat atcaagattt
    20581 ctcgattacc caaaagaaat gcccagaatg tggggccaat ctgcgcgaga aaaacacacg
    20641 tgacggcaaa atctatgtct gcaccaattc agattgcaac taccgcagaa gaaaagatcc
    20701 aaaagtctct aatcatcgat gcccgcaatg tcaccggaaa atggaaatca tcgaaggtaa
    20761 aaacggtgct tatttccgtt gtaaatacga tggaacgaca gaaaaattgc ttgataaaaa
    20821 agagcagaag aagaaaatga ccaaacatga agaacgacgc ttgctgaaaa aatatgcaca
    20881 agcagaagaa ccagaagaaa gcccattagc agcagcacta aaagcagcaa tgaagaagta
    20941 ataagatatg aaagaggttg tgatagaagt ggtcagcttc aagaaataag aaggaattta
    21001 cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgaagaa gctacttctg
    21061 gaacaccatt tattcggaaa aaaggactgt gacaagactt ttgtcacagt cctttttcat
    21121 ttaaaaaaat gtttacttca tttgacgtat attattggtg aggcttcaat tcatttggct
    21181 tcactttttc tgtttctctt atgatataaa caggacgctg tttcgtttct aagaagattt
    21241 tcccaatata tttcccgatg attccaagac ataacaattg gattccacca atgaacagga
    21301 agatcgagac catggaaggc catcctgatg taggatcacc aaacaacaat gcccgcaaaa
    21361 taatgaacaa aattgcaata ccagaaccga cacaagacaa agcgccgata aatgaagcaa
    21421 tcgttaacgg tgcatccgag aagttcacga taccatcaat cgagtagttg aataatttcc
    21481 agaaggacca agatgtttcg ccagccacac gttcgcggtt ttcataagaa atatattctg
    21541 ttttaaatcc gacccagcta aataatcctt ttgaaaatcg gttgtattct gttaattcta
    21601 ggacggcatc caccatttga cgagtcatca agcggaaatc acgagcgcca tcgaccattt
    21661 ctgtatcgct gattttattg atcaactggt agaatttttt cgcaaagaaa ctgcgtatcg
    21721 caggctcgcc ttcacgagtc gaacgccggg tgccgacaca atcgatatca gaagtagtga
    21781 tccggttgat catctcagga agcagttcgg gcggatcttg cagatcaaca tccatcacgg
    21841 tcactaaatc tcctgtagaa gcttgcaatc cagcgtacag agccgcttct ttcccaaaat
    21901 ttctggaaaa tgagacaaag cggacataag gataagctga ggctagcttc ttgattgctg
    21961 ctaacgtacc gtcttttgat ccgtcgtcaa taaacaaata ttcgaactca tgggcacctt
    22021 tgaatttttc aacctcatca aaaaaaagag gaacagtttt ttcttcatta aaacatggga
    22081 caacaattga tactttcatc gtttctatcc tttcaatcgt atgtttatat atggtttaca
    22141 ttcaaaattt aataaacctt aaataacacc tttaaaattt gattttacgt aaaagaacag
    22201 gtattctttt tgtaaactaa cgtatttagt ttaccatgtt tcctcggttt atcaaacttt
    22261 taagaacaat ttttattagt tggcaagtag ttttggatag agaaaaagga ggaagttcca
    22321 gtctattgac taggagcaaa caaaatgaaa atacacacac ctatcggctt tttatggtta
    22381 gatgcagatg aatccggatt gactgctgta tcttttcagc caatttcaga aagcgaatca
    22441 gcgaatcagc gaattttaac gcgcacacaa gaacagtttg aagaatattt tgcgggaaaa
    22501 agaaaagaat ttgatgttcc tttatctata aagagaggta cgccttttca gcaaaatgtc
    22561 tggaaagctt tgtcgaccat tccttatgga gaaatccgaa cgtataaaga agtagccgtt
    22621 gctgttgata gtccgaaagc tgtgcgagcg attgggcagg caaatcgaac caatccattg
    22681 ccaatcgtta ttccttgtca tcgagtgatc gggcaaaatg gaaaactgac aggatatatg
    22741 ggaaacgcaa tagatggggt tgaaatcaaa cgccaattgt tgttgttaga aggatatctg
    22801 caaaattaaa aacagttgtg attcggacat tgttccatgc gagaatcaca actgtttttt
    22861 catgttcatt ctaaactttg atcagccaaa taaatagtaa agccatcact gtttggagaa
    22921 gcccgacagc taatccataa gataagaagc gtgtaccttc tttcatgaat tttgcaaagt
    22981 ctaaacgcag accaattgcc gctaaagcag tgatctcaaa ccagctgctg ataaaatgtg
    23041 ccgattggtc aataactaca ggtaaagaaa acaagctgtt gaccaaacaa aagatcacaa
    23101 accctaatac ataccaagga agcggtaatt ttcgtttttt ctgcgtatca gttggcgttt
    23161 caaacagagc tggttgtttc ttgtgcatga atttttcaaa cagatagaca acaacaacga
    23221 gcatcataat acgtagaatc ttaaatagca tggcgaattc gacaacagaa ttgtcaagta
    23281 agcttgcccc agcgactact tgaccaacag attggagtgt accacccaaa agtgcacttt
    23341 tttcaatgag atttccacca aacaactgta atccaagaaa agggagagtc aacatcatca
    23401 cagtacccag tagattgact agtgtgatga tctgtccttt ttcttcctct ttcgcatgga
    23461 tggaaggggc gattgaagca atagcagaag aaccacaaac agcgtttcct ccagccatca
    23521 tcaaagaaga agtatcgctg aattgcagtt ttttaccaat caagtaagca ccgatgatag
    23581 tgactgacat ttgcagtaac acaaaaacaa ttccttttac accaatttga gcaatcgttt
    23641 gagctgtgac agttgcacct agtaaaaaaa cagagaattc taatagtttg ctttcagcaa
    23701 attttgtgcc gattgctaga acaggctgtt taaaaaaagt atttcctaat aaaataccca
    23761 ataaaatagc aatagtcgct ccacctaact gtggcagata aatagcaaga agcctgctga
    23821 aggcagcgat ggcaaaagaa agcagtaaac cgggtaagat ttcgcggacc gctgaatcct
    23881 ttacagattt tattggatgt gacaatgatt tcatttgatc ggttcctttc gtcaataaat
    23941 actaaaagca tactcgaaca tagtagcact aaaccagtat tttgacaaaa gaacaagagg
    24001 tcgtgacaaa agtcttgtac gacctcttgt ttcgaataaa cggtgttcca gaagtagctt
    24061 ctacggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta aattccttct
    24121 tatttcttga agctgaccac ttcttttacg acctcttggt aaacggtgtt caaaagcttg
    24181 catctgcggt aataagccca accagtacaa aaacatgaac agctgttttt gtttggccgt
    24241 tcttattact tgatgcagaa cagctttttc acaacctatt gaatcgatta ttaagcgatt
    24301 tttttagaaa caaattttcc atgtttgcta tgttctttaa ttaagaaacg tacgtaatga
    24361 gcaagaacgt agaagacaac tacacttgca aacagacatt ccgcaacttt taagaaatcc
    24421 aatacgctac tatagatacc taaactgttg aagtaatgaa cagtagtaaa tatagctgtt
    24481 gcagagataa caagcgggaa tgtaaaggct gcatagcttg gataaaaacg aagcttgaac
    24541 aaacgtggaa gcatagacag cacaacgaaa tacaatactt gagataaagt gaacaatgct
    24601 acgacaagat aaaaattagg ttcagcaaag tttttaagat aacctgttag gcaaagagaa
    24661 ccaggtgctg caacgatcgt aatcagcgga cgtgtcggat gagccatatt tcttacgaaa
    24721 aagacacgtt tgatgatgat cggcaataag aacgcatagc agatcaatga aatccaaaag
    24781 aaaatttttc caataagtgg gaagaagttc ccggcagtta cagtgatgac cccaaatcca
    24841 acgtaaatga taaaccagct tggataaatt gcttcaattg aaagatcagc tttgataaca
    24901 tggtgataat tgaaataaaa gacaaggatc acttggaaga taacagcgca cagccaaatg
    24961 aatttgacaa ttggtgcaat actttcatat gaaacaaaat aagtacaaat aaccatgagt
    25021 gacatcgtaa aagttggtga aacggaggcg atgattggat catgcaacgt ttgtttcgtg
    25081 tgttcgaata agataaacaa tttaacaaaa atgaatatca taagaatcat agagataccg
    25141 ccagttagat tgcctaaagc aacatggtga tagtctttaa acagatttcc aagggaagct
    25201 aacccaagga tcaacccgca gatcgggatc ggaataattt tcaaaaagtt cttcatatat
    25261 aatattctcc tttttattta atagatttta gtaccaagat gaacgaatac attgtaaaat
    25321 gaataggaat gtatgattac gagcaagcag acagctgatt gtcttcatct aagttactag
    25381 ttggatcagc aactgttttg ttgtacattt ctattataga tggttttttg tatatttaaa
    25441 tcaaatagtt tttatagtaa ttatcataaa aagttatgga agtggtgcca tgtttaagtt
    25501 attaacgact tttcgagcag tatatgaaac aagaaatttt tccagagcag cagaagtact
    25561 gtttttatct caaccagcag tctcgaatca aatcaaacag ttggagcggg aattgaatat
    25621 cgatcttttt caacgtaacg gaagaaaaga gatgcttcca actaagcaag ccgacttatt
    25681 atatgaacgg acactggttt tgttagaaga atgggaagaa actcagcaaa aattgctcaa
    25741 tgaacaaaat gaagaagaag tatgtcggat cgttgcttcc cacacctttg ctgtctatct
    25801 tttgcctcag ttgatgaaac atctgatcaa aaggtttcct aaagtgcatt ttgagatcga
    25861 aatcacgaat tctcatgaat cattagaaca agtagcgaaa catgaatccg attttggttt
    25921 tatcgaaaag ccattagaga cttccggcgt acaaagatac tctttgatgg aagaccagct
    25981 agtcatcgct ggggacccaa aaagcaaatt gtggcttgta agagaagcaa actctggtgt
    26041 ttttcattac acgcagcgtt attttgaaga gatgaatatc caagataaga agatgatcgt
    26101 gaaaagtaac gatgtaattg ttgcgttgct gaaggaagga atcggtcgct cgattttgtc
    26161 taagcgctct gttccagaaa atgtgccaat gactccgttg aatttccatc gatattttta
    26221 tttcatccaa cgaaccaatc tggtttctga tcagctgcta gagattgccg aagagatcct
    26281 tcgatacaat gaaagcacag aaacttctca cagcgaggag aatctcagct ggtagataat
    26341 tgcatgtatc aaaaaaagtt ctatgataca ctaaagataa gcagtaggct tttgtactgc
    26401 atttgctcaa gcaactgcac aaacggaaag ttaatttttc ccaaatgtct atggaaagac
    26461 tttccattat ctaactggac cgctataata atcagcagta tggtttttaa gaaaagggat
    26521 gaaacatgga aaaaaaatta aaagcttcga acgataatcg tattgattat ggcgtgattt
    26581 taccagtctt tctgttatgt ttgattggtc tgctctcatt atatgtagca cttagccatg
    26641 atccgaacaa tccagcagtc ttgaaaggcg tagggatgca agttgtctgg tatttagttg
    26701 gagcggcagc aatcgttgtc atcatgcatt tgaattccaa atggatctgg aaactgactc
    26761 cttaccttta tggtttgggg ttagtggtaa tgggactctt gctcaaattc tatgatcaga
    26821 atttagctgc ttcaacaggg tctaaaaact ggtttcgatt cggtgcgttt actttccagc
    26881 cagcagagtt gatgaagatt gcgtacatct tgatgatggc actgatcgta actaaacata
    26941 atacgcaagt aaaagaacgg acgatgaaat cggacttttg gctgattggg aagctcttga
    27001 tcgtgacaat accggtttta gcattgatca tggcccaaga tgactttgga acgatgcttg
    27061 tctttcttgc gatctttggc gggattttcc tgatgtctgg aatctcttgg cgcatcatcg
    27121 cacctgtagt aatcttggca gtagtcgttg gagcaggaac gatcttcttg gttacgactg
    27181 aaggcggaag agacttgctt tataaagttg gattcaaatc ttatcagttt gcacgtattg
    27241 attcatggct ggaccctttc catgatacgt caggaatgag ttatcagccg gcccaaggac
    27301 ttttggcaat tggtaccggt ggcttgtttg gtaaaggatt caacgtaagc aatatctatg
    27361 tgcctgttcg tgagtccgat atgattttta ccgttatcgg tgaaaacttc ggctttatcg
    27421 gtggtgcatt cgtcattttc ttgtatttta ttttaatcta tcgtatgatt cgggtgtgtt
    27481 tcgatacgaa taatgaattt tacgcctata tcgcaagcgg tttgatcatg atgctgctgt
    27541 tccatgtctt tgagaatatt ggagcaaata tcggattact tcctttaaca gggatcccat
    27601 tgcctttcat tagtcaggga ggatcctcga ttttagggaa catgatcggt atcgggttga
    27661 tattatcgat gagataccaa aatgaagcac ctgcaagatc ttcaaggagg taagaaaatt
    27721 gtacaccttt tactggtacc cgaaatgttc aacatgcaaa aaagccaaag cgtggttaga
    27781 ccagcatggc gttagttatc aaaccgttga tatgatcaaa gaagtaccga aggcctcaac
    27841 gattgagact tggctagaaa cgagtcaagt accaatccgt cgcctgttca acacaagcgg
    27901 gatgaaatat cgggaacttg gactgaaaga tcaagtaggc gagtttacgg cttcaaaagc
    27961 gagccaagtc ttatcgacag atggtatgct gatcaagcga ccactgatct tacgagagga
    28021 ccagcttgtt gcaattggat tcaatgaaaa aatttatgaa ggagaactga tataaaatgg
    28081 caggcaagca tttgaagaaa aaggacaacc tttggatctt atttaatgga aaagaatact
    28141 gtgttggttt gacaaatgaa gcgcaagaag aacttggaga catcacgttt gccaatctgc
    28201 caaaagcagg acaatcgcta aaaaaagatg aacctctgat cgaagttgaa gcggaaaaag
    28261 cggtcaatga atttgccagc ccgctaacgg gtacagtttc ttctgttaat gataaaatcg
    28321 gtgaagatat caatatcttg aatgattcag atgaaatgaa tgcgtggatc ttaagtttca
    28381 aagatgttga tccaaaagaa tttgacgaat tataaaatca gaaaatcact tgaaaaaagt
    28441 tgacactgat tcttccagtt gctataatca taagcaatcc tatatgaaac gagaaagctt
    28501 tgaaaagaag agtacatgtc acggacgttt gaagagagcc ctgtttgctg aaaatgggta
    28561 acgaaaatga gatggaagat ggtcttagag cagagtaagc gagcgtaagc aaacttacta
    28621 cgggagtgcc cgttacagcg cgacagtata cggaaagaag ctaagtccaa gtactggaag
    28681 aggctgttat tgcgaagtaa cggtaaatca aggtggtaac acggaagttc aagctttcgt
    28741 ccttttgctt aaggcaagag ggcgaaggct tttttgtttg aaaaattata agagaaagat
    28801 gtgaatggca tgtcattaat ccaactaacg aatgtcaaaa agacattcaa tgggaaaaac
    28861 ggaaacatcc aagccgtcaa tgacgtttca ctaaacgttg aaaaagggga tatttatgga
    28921 atcgtcggct attctggcgc aggaaaaagt acattagtcc gtctcctgaa tggacttgag
    28981 cttcccacga gcggcgaagt gatcgtaaac gatcaagata ttacaaaatt aaaaaacaaa
    29041 gaattacgta cattccgaaa gaaaatcggt atgatcttcc agcatttcaa cctgttatgg
    29101 tcaagaactg tacttgaaaa tatccagctg ccgctggaat tagctggcgt accaaagagc
    29161 aaacgtaaag aacgggcaga agaactgttg cgtttagtcg ggttagaagg acgcggacaa
    29221 gcatatcctt cacaactttc cggcggacag aaacaacgcg tcggtatcgc acgagcattg
    29281 gcaaatgatc ctgagatcct gctttgtgat gaggctacta gcgcattgga cccgcaaaca
    29341 acagaagaag tattagactt attgttagcg atcaataaga aattgaattt gaccatcgtc
    29401 ttgatcacac atgaaatgaa tgtgatccgc aaaatctgca acaaagtcgc tgtgatggag
    29461 ttggggaaag tcgtagaaga aggagacgta ttgactgtct tcagacaacc gaaaaaagaa
    29521 gtaaccaaac gctttgtaca acaagacatc gaaccagaag ctgattcgac agaactatta
    29581 gctgaactca tcaaagaaaa tccaagcggc cgtttggcaa ctttgacctt tagcgaaaga
    29641 aatgccaacg agccagtcat ttcccaagcg attcgacgct ataacatcga tgtcaacgtt
    29701 gtgtatggaa agatcaaaca agtgaaagaa ggttcctttg gttcattgac tgtcttgatg
    29761 actggtgaaa ccaaagaatt agatcaagca gaagtgttca tcaaagaaca aggagtagga
    29821 attgaggtga tccatcgtgg ataaaagctt ttcagaaaca tatctagatt ttaaacaaat
    29881 caacccggaa acgatgaatc aggctatcct tgatacacta tatatgacat taatctcaat
    29941 ggtcttagtg acgatcatcg gattagtttt gggattgatc ctttactcta ttggacgcaa
    30001 aaacaaaccg tatgctcgaa tcatttatgg aatcgtatcg atcatcagta atattttccg
    30061 gtcaacgcca tttatgatcc tgatggtttt gatcattcca tttactaaag caatcgtagg
    30121 cactatgcta ggtgcgcaag cagccattcc agctcttgtt ttatccgcag caccatttta
    30181 tgcccgtcta gtagagattg cttttcgtga agtcagccct ggtgtactgg aagcagcaga
    30241 tgcaatggga gcaagttact gggaaatcat ctggaaggtc ttgatacctg aaagtattcc
    30301 cgcattgatt tctggattaa cagttacaac gatctcgatg atcggattta ctgcgatggc
    30361 cggcgcgatc ggcgcaggtg gacttggagg tcttgcttgg caggaaggct accagcgtgg
    30421 taatctaaca gtgacattcg ttgcgacttt aattatttta ctaatcgtat ttatcgttca
    30481 aggaatcggt gatttcttaa ctaagaaaac agacaaaaga taataggata tagggggatg
    30541 taggatatga aaaagaaaat ttttggattt gcagcagcgc ttttgctgac tgtaggatta
    30601 gcagcatgcg gtaacaacag cgacagcaaa gattcagcaa ataaagcaga cgacacgaca
    30661 ttgaaagtcg gcgcttcacc gacaccgcac gcagagattt tggaacatgt gaaaccatta
    30721 ttgaaagatg aaggaatcga tcttgaaatc gtcaaattcg atgactatgt attaccgaac
    30781 caatctttgg aagaaggcga cattgatgcc aattacttcc agcatattcc ttatttgaac
    30841 aaagaaatca aagaaaaagg atatgacttt gtcaatgccg gtgctgtcca catcgaacca
    30901 atgggcttgt actctaaaaa agtcaaagat gtttccgaat taaaagatgg tgcaacaatc
    30961 attacttcaa attctgaatc agactgggga cgcatcatca caattctaca agatgctggc
    31021 ttagtcaaag tcaaagatgg agtagatctg gaaacagcaa cattcgatga tattgaagaa
    31081 aaccctaaga acttgaagtt caaccatacg atcgacccat cacttttagc ttctacgtac
    31141 caaaatgatg aaggtgacct agttgcaatc aacgcaaact tcgcttacgg cgcagggttg
    31201 aatccattag aagatgccgt attgcttgaa aaggacaact ctccttacgt gaatattatt
    31261 gcgactcgta aaggtgatga agacagtgac aaaatcaaaa aactgattga agtgcttcac
    31321 cgcaaagaag tccaagaatg gatcacagac aaatggggcg gctctgttaa accagtaaag
    31381 gctgatgcaa aataaggtat ggaacaaaaa agatgagaaa taagtctgta atggcttatt
    31441 tctcatcttt tttataccta ttctaattta gaaatcaaaa ttgtttaata ataaataaaa
    31501 gatatttgat tttagtaagg aaggtcattg cgcttcattc tcaattagtc taaaatgagt
    31561 gagaacgaaa gagaatttgg gggtataatg catgtctgtc ttagaaataa aaaatctaca
    31621 tgtgtcgatc gaggaaaaag aaatccttaa aggcgtaaac ctaacaatga aaaccggaga
    31681 aatccatgcc atcatgggac ctaacggcac aggaaaatct accttatctg ccgctatcat
    31741 gggcaatcca aactatgaag taacagaagg cgaaattttg tttgacggga aaaatgtttt
    31801 agacttggaa gtagatgaac gtgcgcgact tggcttattt ttagctatgc aatatccaag
    31861 tgagatacca ggtatcacga atgcggaatt catgcgtgca gcgatcaatg cgaaacgtga
    31921 tgaaaacgac aaaatttctg ttatggcatt tttgaaaaag ttagatgaaa aaatggcttt
    31981 actaaatatg ccagaagaaa tggctgaacg ttatctgaac gaaggttttt ctggcggaga
    32041 aaagaaacgg aatgagatcc tgcaattgtt gatgttagaa cctacatttg caattttaga
    32101 cgaaatcgat tcaggtctgg atatcgatgc tttgaaagtc gttgctaaag gagtaaatga
    32161 aatgcgcgga gacaatttcg gtgcattgat catcacccac tatcaacgat tattgaacta
    32221 tatcacacct gatgtcgttc acatcatgat ggaaggccga gtagtcatga cagggaatgc
    32281 tgacctagct aaacgtctgg aagcagaagg ctacgctggt atcagtaaag aattaggaat
    32341 cgactataaa gaagaagaag cgtaaggggg agcaagaatg aaagaaaaaa actggctcga
    32401 ttatcttgac gcggtaaatg atttttcttt aagtaaaggc gaacctgatt ggatgagaac
    32461 cttccgtcaa gacgcactag cgaaagcaga tgaactgcca ttgccgcata tcgatcgggt
    32521 gaaattccat cgctggtcgt tatttgatgt gaaagaaaca caaaccatca gtgaaacagg
    32581 cacgattccg gcatttgatg caatgaaaga caatcctgtg cttgtccaac aaggttcatg
    32641 gacgattttt gaacaactgc ctgtggaatt agcagaaaaa ggagtcattt ttaccgactt
    32701 gttcactgca atgatcgaat atccagaact agtccaagaa tattatatga aaaaagcagt
    32761 aaacatgaac gaagaccaat tgactgcact ccatgtagct tttatgaaca gcggtatttt
    32821 cctgtatgtg cctaaaaatg tcgtgatcga tgaaccactc gagtccttat tcattcaaga
    32881 tggcgcatcc gatgagcact tcttcaagca tgtgttgatc gtggcagatg aacatagtga
    32941 attttcttat ctggaacgtt tccagacaac caaagaacaa gtagcaaaat catcaggtaa
    33001 tatcatcgtg gaagtcatcg ctaaagctgg ttcaaaaatc aaatattcag ccgtagatca
    33061 attaggagaa aatattactt cttatatgaa tcgccgtggg cacattttac gagatgcttc
    33121 tgtcgactgg gcaattggtg tcatgaatga cggtcatgtg atcgctgact ttgactcaga
    33181 tttagcagga gaaggtgcac acgcagaagt caaaatcgtg gcaatcagct cgggcagaca
    33241 aatccaagga atcgatacac gagtaaccaa taaagcccct catacgatcg ggcatatttt
    33301 gcaacacgga gtgatccgag aaaaaggaac attgaccttt aatggtatcg gccacatctt
    33361 gaaaggggca aaaggtgcag atgcccaaca agaaagtcgt gtcttgatgc tttctgataa
    33421 agcacgtggt gatgcgaatc ctattctttt gatcgatgaa aacgaagtaa ctgctggaca
    33481 tgctgccagt gtaggacgag ttgatccaga agaaatgtac tatctgatga gtagaggatt
    33541 gcataaagaa gaagcagagc gcttagtgat tcgaggattc ctcggttctg tcttaacagc
    33601 aatccctgta gaacaagttc ggaaagaatt agtcgaagta atcgaaggga agttgaatgg
    33661 atgatcgatg ctaaaaagct acgcaatgat tttcctatct tatttcaaga cgtcaatgat
    33721 gaacctttag tttatctgga taatgcagcg acgacccaaa agccaaaaca agtactccag
    33781 gcattagcgt actattacga acacgataac gccaatgttc atcgcggtgt ccatacgtta
    33841 gcagaacgcg cgacccatga ttatgaacaa gcaagagaaa aagtacgggc gttcatccat
    33901 gcaaaagaaa cagcagaagt gctgtttact cgtggtacga cgacaagctt gaattgggta
    33961 gccagaacct tcggcgaccg ctatatcaaa gcaggagacg agatagtgct ctcttacatg
    34021 gaacatcatt ctaatgttat cccgtggcag cagttggcaa aacgaacagg tgctgtcttg
    34081 aaatatatcg aactaacaga agaaggcttc ctggatatgg aggatgcagc gaaaaagatt
    34141 acggacaaaa ccaaaatcgt ctccattgct catgtatcca atgtcttagg tgtcttcaat
    34201 cctgttcaag aattagctga acttgtccat gaaaacggcg gggtcatcgt agtagatggt
    34261 gctcaatctg ccccgcacat ggccattgat gtccaagcac tagactgtga tttctttgct
    34321 ttcagcggac acaaaatgtg cggaccaaca ggtattggtg ttttatatgg taaacgaaaa
    34381 tggttagaag aaatggaacc tgttgaattt ggtggcgaaa tgatcgattt tgtcgaatta
    34441 tacgacagta catggaaaga actaccttgg aaattcgaag cagggacacc aaatatcgcc
    34501 ggcgctatcg cattaggcca tgcaatcgat tatttgcaaa aactcggtat ggacaatatc
    34561 catcgatatg aagaagaact agccgcttat gtgttgccaa aattacaagc aattgatgga
    34621 ttgactgttt atggtccaca agacccgaat tttcggacag gagtcgttgc attcaatttg
    34681 gatgggttac atccccatga tgtcgcgaca gctttagaca tggaaggggt agccgtcaga
    34741 gcaggtcatc attgtgcgca accgctattg aagtaccttg aagtatcagc gactgctcgc
    34801 gcgagctttt atttctacaa cacaacgcaa gacgccgacc gtttagttga agcaatcatg
    34861 gcaacaaagg agtttttcca acatgggact atctaaatta gacaatttat accgtcaagt
    34921 gatcttggat cattcttccc atccgcatca tcgtggaagc ttgactgact cgacaagcaa
    34981 gatcgaattg aacaacccca catgtggaga tgtgatcgaa ttgcaagtcg atgtaaaaga
    35041 cggaatcatc aaaaatatcg cctttgacgg cagtggctgc tccatcagta cggcaagtgc
    35101 ctcgatgatg acagacgccg tcatcggaaa atcaatagaa gaagcagaac atttaactgt
    35161 agactttcta cagcttgtcc aaggaaaaga agtcaaagat cccgaccaat taggcgatgc
    35221 tgctatgctt ggtggtgtag ctaaatttcc cgctcggatc aaatgtgcga cacttgcctg
    35281 gaaagcacta ggaaaagcaa tggaaaacga tggtaccgca gctttccatc attaccacaa
    35341 ggaagaggag tgagaacatg ggcgtaccag aattagaaga atataaattc gggtttcacg
    35401 atgatgtcga accggttttc agtacaggag aaggcttgac agaagaagtg atccgtgaga
    35461 tgtcaagaat caaaggcgaa ccagaatgga tgcttgaatt ccgtctgaaa tctctagaaa
    35521 catttaacaa aatgccaatg caaacatggg gccccgacct ttcagacatc gatttcgatg
    35581 ccatcaaata ttaccaaaaa ccaagtgaca aacccgctcg tgactgggaa gacgtaccag
    35641 aaaaaatcaa agatacattt gaaaagattg gtataccaga agctgaacga gcttatctag
    35701 ccggtgcttc cgcacaatat gaatcagaag tcgtgtacca taatatgaaa gatgaattcg
    35761 aaaagttagg aatcgtcttt actgacactg actctgcttt gaaagaatac cctgaattat
    35821 tcaaagagta cttttcaaaa cttgtaccgc caaccgacaa caaattagca gcgctgaatt
    35881 cagccgtatg gtcaggtggg acctttattt acgtgccaaa aggcgtcaaa gtcgatgttc
    35941 cattacaaac gtacttccgg atcaatgcgg aaaacacggg gcaattcgaa cgtacgctga
    36001 tcattgttga cgaaggagca agcatccact atgtcgaagg ttgtacggca cctacttatt
    36061 caagtaacag cctgcatgcc gccatcgtag aaatctttac aagaaaagat gcgtattgcc
    36121 gttacacaac gatccaaaac tggtcagaca atgtgtacaa cttagtaacc aaacgagcaa
    36181 aagcatacga aggcgcaacc gttgaatgga tcgacggaaa cctaggtgca aaaacaacga
    36241 tgaaataccc aagtgtttac ttagatggaa aaggtgcacg aggcaccatg ctctccatcg
    36301 cctttgcagg agcaaaccaa atccaagata cgggtgcaaa aatgatccac aatgcaccaa
    36361 atacatctag ctctatcgtc tctaaatcaa tttcaaaaga cggcggagaa gtaaactatc
    36421 gaggacaagt aacctttgcc aaaaacagtg cgggttcgat ctctcatatc gaatgtgaca
    36481 cgatcatcat ggacgaatgg tcgaaatccg atacgatccc attcaatgag atccacaaca
    36541 gccaagtctc tttagaacac gaagccaaag tctcaaaaat ctcagaagaa caactctact
    36601 atctgatgag ccgaggcctg accgaatctg aagcaactga aatgatcgtc atgggctttg
    36661 ttgaaccatt caccaaagaa cttccaatgg aatatgcagt cgaactcaat cgattgatca
    36721 gttatgagat ggaaggcagt gtaggttaaa cctgttatag gagctagaac ccttgatata
    36781 taaggattct agcttttttt gttttgcctg tatactagaa atgaccagct tttgaccagc
    36841 tcttttactg atgagccatc atatcagcaa agtcttgacc tgttttctta ggtgcattat
    36901 ttgtaatatg tgcatagatg tctaatgtca ctttactatt ttcatgccct aatcgttgtt
    36961 gaactacctt gacagattct cctgcttcta ataacaaact agcatgtgta tgtctaaacc
    37021 catgaggtgt aatacgaggc aagttatatt ttttcaaaat atatcttaac caatcattaa
    37081 cagtgtttgg aatgtataga gtgtttttaa ctgttgtaaa aacatgttgc tctttagagc
    37141 tagtattgta tccaagtttt aaatactctt ctttttgctg taatttccag ttgttgagga
    37201 tagaaagagt ctcagtatct aaagagataa ctcgtctaga agctcttgtt ttcggagatt
    37261 gaattataac gttaccaaat tcatctacag caagagtttt attgacatgt agttctttat
    37321 taaacgtatc tatgtctttc cactgtaaag cgagtacttc actctttctc atacctgtgt
    37381 atgccaataa tctgaagaag gcgtattgtt tcatgttacc aaaatcttta aaagcatcca
    37441 gtaaatcatt aagctcctct tttgagtaaa atttacgatt tttctcatac tctttttttc
    37501 gtggaagaag tgttttttcc ataggattat tgtcaatata ttgcattgca accccgtagc
    37561 gtaaaatttg tgcagtctgt tttctcatat agtcgtaact ttcatattta ctgtgccatt
    37621 catctaccac tttttgacaa tatgggacag agatattcga taatttaagc ttaccaaatg
    37681 ctggaagtat ttgattattt gcgtatctta ctgaaattgc gattgttgaa gctttaacac
    37741 tggtacgctg atgagctatc cattcattat acaggtcttc aaatgtcata tcaggtgctt
    37801 tctttttaac accgtataat atttgatgtc tgtattcgtc ataatctttt tgagcttctt
    37861 gtttgctact aaaacctctt ctcgtagtat ttattttttc accagttcgt ggatcggtgg
    37921 caatgtgtcc tttatacatc cacgccactt gaccgttttt ctttttgtac tttgatattt
    37981 ttgccatgaa atcatctcct cttagtttac aaggagtact taccactaga tggtatcacc
    38041 tcccttcatt tttttcttag tgtatttttt cccaattgat gaaatcgttt gttctagctt
    38101 gtttatatta tcagtaattt ctctcacagc ttcttcggga taagacatgt ctttcaattg
    38161 aataggtagc cattcatcta ccattctttc tagtagttct tctatactgc tagtgaggaa
    38221 aagaatgtta ttgtcaaatt cttctacaat agcattgatg aataattctt ctatttttaa
    38281 gatattgaaa agactaattt tttcattttt aactttatta caagtcttgt tagcaataaa
    38341 ttcttgcatc tctggttcta atttggaata tctatctagc catgtatcta aattagagtg
    38401 tactggtggg aactgttgac gaagatgctg gaacaggttt ttatctggaa atgttatacc
    38461 atctagtaca atgctatctc tataaagaat aattcccaaa atatattctt ctacattgcc
    38521 acaaactaac tcctctatac tcatatttct caaatcagca attcgggata atcgctctgg
    38581 tattggtaaa taggttccct tttcccattc gtagatagtt tttttgtcaa tcaattgacc
    38641 gttgttaatc ataagaccaa atgctggttg tgttaatttt ttactctttc ttatagcttt
    38701 tatttttgtt ccgagtatct tttttcgttc taaataattt attggtggac gctttatcat
    38761 attgaccaca cctcctaaaa ttagtatacc ttaaaaaagg ggtgttgtaa ataaatttaa
    38821 caaaaatatt gacacccctt tttcgtcgag gtattatgtg tatgtcgatg aactggtaat
    38881 cattttttgt ttgattaatc ggttaataac aaaatacttt tattttgagc aaaaaatacg
    38941 aagatgattc ttagaaaatt tgttcaagca atcacgaaag gaggggaaaa tatgtctcta
    39001 caaatcattg atagcattgt ttctatacaa gttgaacaac aagtgaaaga gataattaca
    39061 gtgctaaagc ctatgctaga agacattatt tctggagtca ctctagagta tatggactat
    39121 cagcaggcaa gtgaatatct aggtgtatca gtaggaacga ttagaaaata tgtttctcaa
    39181 tacgggttac ctgttataaa aattgatacg gtcattagat tcaaaaaatc tgatattgat
    39241 agtttcatgg aacagtataa aggttgagta aacaaataag actaaaacat atagtaataa
    39301 agagtttgtg tttattatct gtatgtttta gtctttaaca gaattttata aggaggaaac
    39361 acaaatggaa acaccaatga ttaatattcc aattaatgta gataaagaaa gactattaga
    39421 gaaaccagtt atagaggatg aaaaaaagag aatacgaaaa agatgtttag ataactacag
    39481 agaagttaca agagatgagt ttgtagagat actaaattca tcacaatcat ttattccaag
    39541 taagacaaag aataaaggca acaaaagtga agagttttta gaaactagag ttattattct
    39601 ggatgtcgat aacactgtta aagatgagaa ggggaaggta gtagatttaa gtaaagatga
    39661 tagtagatac ttaagcatag aaaaggcttt atctatcaat ttagttcata attctgcgtt
    39721 tgcgatacaa aaaagtattc ggtactctga aaatctagaa aaattcaaga ttgtctttct
    39781 attaaaagaa gcaattactg actatagtaa aatggttgct gtttacgaat acttgaaaaa
    39841 aatcataccc gggtgtgatg acaatgtaaa tgcttcgaat agaatgtttt ttggaggata
    39901 taagtcagat gcagtgataa tgattaatga agacaacatg ttagatatta ctgagcttcc
    39961 gattgatttt accatgacat gttctaaaaa ttgtcaaagt gattcaggga gagagtatga
    40021 attagtcaat gaaactgatt ttgttaagtt gataaaaaat gacgacaaag aagaaatgaa
    40081 acagtggttt aaagactgct tgtttgattc ctccgacatg tctttcaatg agatttatga
    40141 aagattgtta accattgata tggataaatt acttaagtca agtaaaaact taagatgttt
    40201 gtttcacgac gaccataatc cctctgctag tatttttacc agtaaaactg ggcattccat
    40261 tttttattgt cattccagta attgtagtat cagtgaaaat tttattgggg ttgtgatgtt
    40321 aattctaaag aaagacagtc gggtagaaac atttaattgg ctattagaga acctagacct
    40381 gtatcctaca cattttcgaa aactgaaaga ggaatcaaaa gtactttttg acactcttga
    40441 atcgatggaa gacaagcttt tcaggacaat aaggaattat ctagaggaaa tgagacatgt
    40501 ctatgatatc ttgctttctg atgttaattt ttttgatgat agtaaggaaa gtgtgacttg
    40561 tattttgtcg ggggaacaat tagctaaaaa aatgagttct tactatggta aaacttatga
    40621 catagataaa tgtaataagt tactatcact tatgactttt ataggattga taaaaaaatt
    40681 agatgatgaa gatgttcctt tgaagatgct cgaataccta aataggttaa aggaagagaa
    40741 agcgttagga aactttaatc ataagagaag caatgtctat cgattagacg tttttaatgc
    40801 aacttctgtg attcaaaaat tgaatgaaga tatccttcca cttctggaaa aaattcattt
    40861 tacatatcaa tatttttctt atgaatgggt taagatatgt tttaacgaaa aagaggctaa
    40921 aagagtattt cctcaaaatt cccgatcaga actcagcaag gaaaaacaat tgattttaga
    40981 tgaagcacat gaagttttac agggcttatc gtggcaaggg acatatctaa tgacagagaa
    41041 agaacttatt aacactgttt gtgaacgcct acgtaatgtt ggggataaca gcgtgaaaaa
    41101 tgctttaata aaaaacagag gctactttgt taaacaagga ttcactcttg aacgagcttc
    41161 taaggatatt aagaaactat acaatgtgaa agcaagtagt agttttttaa tatatttagc
    41221 gaaaggggca gggatagatg aaacaataaa agaattgata ttatcaggag ctggggtagt
    41281 tcctctgtat caatttacaa gtgtaacaag agatgatgga cggagagtaa ggaagagaaa
    41341 agacccaatt gtattaaact agacaggtta tccatagctc ttaatcttat ttcatctcaa
    41401 tacacccacc gttaaagttc ggtgggttat ttatttttca atctttcaat agctgattgc
    41461 gttcattttc ttgccttttt aatagcttca tatagtcaat tgttcgaact gcatcttcag
    41521 tgctaagaga acgatagaga gccaaaaact cttcctcata atctacgaca acattgtcta
    41581 gaaaatggct tacaggggtt cctagggctc tagaaagagc tgttaagcta tcgaggttag
    41641 gttctgttac agaattttcc catcttgcta ctgcctgttg tgtagagaag atttttttag
    41701 ctaattcttt ttgtgtccac ccgagagctt ttcgcttttc tctaatgacc tttgcatatt
    41761 gtttttgttt cattaataca cccccttctc agtagagtac aacaagttgt tgtatttgta
    41821 atgatatttg aatttttaat atgttttatg agttgaaata catcatattg ttgtatataa
    41881 tggaacagga ggggagaatt atgaaaaaag taagtaatct cagccgatac agaaaagaaa
    41941 aaggattttc tcaaactgag ttagctaaaa aaatgaatgt tacacagcaa tgtgtaagtt
    42001 cgtggcaaac agggagaacg attcctaaac cctatcaaat gaaaatgctt tctgagatat
    42061 taagtgtacc aataaatgag ctgttttctg atgtatttaa taaagtaaac agttaactaa
    42121 taaacaattg aatgttttta ggaggagaag acatgtattg tagaaaatgt ggcaaagaaa
    42181 taacagatga aagtaagtat tgccagtttt gcggagcact acaagaaaca gatgagacaa
    42241 ctggagcaga cgcagtaaag aaaagtactg agcagacaat taatgaaatg aaggaacaaa
    42301 tcgtacatgg acaagagaca gggaagctgt atttgattat tggttggatt tctatggtag
    42361 tatcgttagc atttattcct gttgtatttg gtgctgttgc tgttattatg ggttacttat
    42421 atcgagagaa agatgaaaaa atgggtacga ttttgatgat agcaggggta gctggagcca
    42481 tctttggtgt acttttaggg atgtcaatgt actgataaga gggataagag caaattattt
    42541 tggagatagg agataaaaag ttgaataaag taaatatgaa caagaagttt gtcattatag
    42601 gtattgttgt cttggctatt ataggtatca ttttttcaaa aatgacaggt acatctggat
    42661 tagagaagtc atcgaaacca ttggtaacac agattttaca agaacagtac agtttaaatc
    42721 ggacatgtga tgatgtgacg attactcatg aaaatggaga taacacatat cgagctaaag
    42781 ctatattaga caatggaagt gctatcaaca taaatattga gtactatcca aagaaagacc
    42841 gtatatatgt agagatacca tatactgagg tgttaatgtt gaattgaacc ccaccccatt
    42901 gatgattgac cgtattttgc atggaaacct tgggaggcat aagctaacta ggggttcctt
    42961 tttcttttct ttttttggaa tctatttaag tgaaatgtat ctgaaatgaa atgggagtaa
    43021 gtgttataat agttaataca taagcgcttt attttatttc agatggtgaa tagtataagg
    43081 aatttttttg atattattac agtttatctt actataaagt actatttttt aaagcaataa
    43141 cagatgaaaa tttgattcat tcataacaac atgtacagct ataccaccta cttggaataa
    43201 ctgaagaaaa tgtacgaaaa accatttcaa gtaaacccca aatgattgtt aatgaactac
    43261 aagggtggtg aagcttcatg agtctccaca gagatgctcc atcaaaagtt ccgtcaagct
    43321 catgaaaatt gtgaaaaacg ttttgaataa tatcaaagaa agagggaatg gaaatggcac
    43381 acaatgaacg ttttaaacaa gaaattattc aagaactcga gaaaggagta caacaccaaa
    43441 aattaatatc aaagaccgcc caaaaatttg atatctcact ggctaaggag gttaaacttc
    43501 atgaagttta acctgctagc tctactaacc gtggtaacgc tcgtttttgt tggttgtact
    43561 aaggctcctg attccttgaa aaattcttct aacagtgcgg aggtaaacgt ttcgacctct
    43621 accagtgaag cggtacagtg ttcacaagaa actactagct tgccagaaga gctacttgga
    43681 gattggcaga ttaataataa ggatgctggt atagagatat cagacagtaa agctgtattc
    43741 cgtataggaa ggtctcaagt agagtcaaag gactattatg tagatgtaaa caacaaaatg
    43801 cttttacatg tctatttttc ttttgagtat gatggtgtga aatacagtga agtctctatt
    43861 gaaagaactg gctatagtga atttattagc aaaagcgtgg gaattttaac tatgcatact
    43921 aaagatggac aatatataag tttgggtgtg aagaaagttt ccggtgagaa taatgagaca
    43981 gaagcagtta cggagcagac gatggatgaa agaattgatt catctgagag taatgagaca
    44041 gaagtagata ctgggaagac gatagatgaa agaattgatt cattcatcga aacatacaca
    44101 gctataccac ctacttggaa acttgtaaat gaagataaag gaatcacgat aaatactgat
    44161 acagagattt ctacagatgc tcaatcttta tcagatttat tagccaacct aacaggaatc
    44221 agtaatgcgg tgacagagca agttggtaaa aatgttccac ttcggttact tgaaaatggg
    44281 aaaaaagatt attctgtggt atttaagaac ggacaggtaa ctgattacaa cgaagtattt
    44341 aaaaaagtta ttgaataatg cttcttcaat ctttttcatt catttacccc accccgttgt
    44401 tatataatcg tatctcaaga ggaaccatta gaaagcatag gctaactagg ggttcctttt
    44461 tcttttgttt ttttggaatc cgcttaagtg aaatgtatct gaaatgaaac gaaaataacg
    44521 tgatttttta ctgtaatatc aaggttttag ctgttttaga taccatgtaa cccaaaatag
    44581 tttattgaga aaataggaga gtatgtgata ctaactagta gggaaaaatg agataaaaag
    44641 taaatggaat catttcagaa ataagtgaaa taagacaacg taataggtat aacatgacta
    44701 ttacatagtt ttagacatga aatattacta caaaatttgt ctaattatgt cattatttat
    44761 ggaggagttt tataacgatt tttgtaataa aaagacgaga aggaatgcat acatgcacat
    44821 cctctcgtct tttaactatt tactttagga attacaacaa ttgctgtaat tcgtcaccta
    44881 ttcgttcaac tattttcgtg actaaagcat ttcccataaa gaataagcgc attctatctg
    44941 aaacttctac tatttccccg gaggataatt ttttatactt tgtccaatca tcaggaaagt
    45001 cttgtagacg ttcggcttct ataggggtca acaagcggta acgaccatct atttttaata
    45061 aatgtgtaga tctattcaca gaaccttctg aagttagcat cgtccgtccg ggcatatcta
    45121 aatcatcata aggagacata ccaccttcgg agaatgtata ggtatgacct tcagcagtga
    45181 ctcgctctat ttttttaggt ccacgaagat attcgaattt ttttatttta ctttcgtcgg
    45241 tcaagtaata cttttcagct acttcatctt caggttgaat gatttctctt aatggagtag
    45301 gtttttcttt atcgtatggt tccgtatcta tagtaaagta acgtccatgt ctcattaccc
    45361 cagtattgta tacttttcca gtaaaattat ctgatatctc tactatgtca ttggataact
    45421 tatacgacgc ttgtctaccc ttataaggag tcttgatgat tggaaattgt cgagcaaaaa
    45481 gtccttcact gaatatatac tcatcataac gatgttcttc ttcaccaagt attttttgtt
    45541 cgaatcgtgc atctatcttg ttcgcaaaac cagtatcatt tttgtaaacg aaaaaaaaga
    45601 cacggcgtct acgttgtcta cggccataat cagcagcatt gataacacgc cattctacgg
    45661 aataccctaa atcattaaag gaagcaagca ttatagcaaa atctctgcca cgttgtttag
    45721 aaggagattt aagtagacga tcaacattct ctaaaatgag atactttggt tttatgtggt
    45781 tcgtagcacg gattatttgc caaaatagaa ctcctttttt accttctatc cccatctcat
    45841 gttttttact acgtgcaact gaataatctt ggcacggaaa tccaccaaca atgatgtcag
    45901 cgtccatttg ctcaaattct tcatttgata tttcttctat attcctgttg atattttcac
    45961 tatttgggaa tcggtagtca tatacttcaa aagcatcttg tgattttcga gaaggctccc
    46021 attggtttgc ccatttcgtt ttgaataggt cggcattaga atgctcgagt ccaactctga
    46081 acccccctac accggcaaaa agttctaata catttagtgt ttcactcata ttggcgaaca
    46141 tcctttcggt tttaacttaa atgtattata caataaataa atcgatttga aaagatcgaa
    46201 atcaaaaatg ctatgattta cttaaggagt ttctcaaggt tattctgtaa agttgaaaca
    46261 acatcaaatg attttgtata gacttggttt ctatgtaacc atgtagtata gttttatata
    46321 ggaataaacg taggatggag tgactgaacg aatgtctgaa tacttaacaa aagagcaagt
    46381 acataataga gctaaagaag cagtggggaa gtctatgata gaattaaata atggaattcc
    46441 ccttctcaat agtaaaaata aaaactctat aggagacata tttgagtctt ggttcggcaa
    46501 ggtaaaggat agcgagagta ggccagacct ggaagaagca ggtgtagagt taaaggctac
    46561 gccaataaaa aagttgaaaa acggtcagta tagcgctaaa gaacgattag tgttgaatat
    46621 catcaactat aatgaggtcg ctgaggaaac attcgaagaa agtcattttt tgtataaaaa
    46681 caacatcatg gaaatcgcat tttatgaata cttaaaagat acgccgaaag accagtggac
    46741 tatccgtgaa gctattttat atgagatgta taaaaaccct gtagattttg aaatcatacg
    46801 acaagactgg gagcttataa agaaatatat tttagatggg aaagctcatg agctaagtga
    46861 aagtttgacg aactatcttg ctccatgtac aaaaggaact aatgctgtga agtcactcag
    46921 gacacagccc tattcagaac aaaaagctaa acaacgggca tactcattga aatcaggata
    46981 catgacatct attttacgta aatatgtatt aggcgatgaa aaaatcgatt caatcgtgaa
    47041 gaatactttt caacttgaag aaaacaactt agaagattta gtattagaac attttatacc
    47101 atatgtaggt caaactgtac aatctttatg tgaaaaattc acaatcaaga catcgtatca
    47161 agctaattat agaatcgttt cttcaatact agggttatct ggtactgtaa ccaaagggga
    47221 acctttccca agagtcgaag aatttgaaaa agcgtctata ctagttaaaa ccatatattt
    47281 tgataaaagt ggaaataaca aagaaagcat gtcatttcca tcattcaatt ttattgattt
    47341 gagcaaacag gtatgggaaa aagaagatgg ggaaccaagc agtgattggc ataatttttt
    47401 attagataca agatttcttt ttgtagtctt taaagaagaa gatgaaggtg ttgtattcaa
    47461 gggagctaaa ttttttagta tgccagaaga agatatagaa gggccagtcc gtaaggtatg
    47521 ggaagatact gtgtctaaac ttcgagaagg agtagagtta gaagctattc ctgataaatc
    47581 tactaaggat ggatggaaaa tcaaaaataa ttttataaag aaaacagaca aaatgatttg
    47641 ccatgttaga ccccatgcta gccaacgaga ttactctgtc gatggacgct atgcggataa
    47701 acttcctgta cccgcaaaat ggattaataa accagacaac gataattact cggatcaatg
    47761 gatgaccaaa caatgttttt ggataaacaa tgattatata aaagagcaag tacaagattt
    47821 gatatcataa aaaaagagga agactactcc tctttttttt gtgcaatata atctaatatc
    47881 acagatgtgc aataatttaa gtttttcaaa atatcttttt cccaaaagtg tataggtacc
    47941 caaccaagtt ttatgagaga ctgattattc ctttcatctc tcctcatatt ttcttctatt
    48001 ttttcaatcc agtagtcccg attccgtttc aacttttgtt ttctattgtc ccaatcgtgt
    48061 ccgtgccaaa actctccatc gacgaaaatt gctagtttat atttggtaac tgctatatcg
    48121 ggagagcctg gtaatttttt atagtttttt cgatacctga caccttgatg ccataactct
    48181 ttggctagca gactttcagc ttttcctccc ttcaagcgta catttgccat acgttttgat
    48241 acttcgggag tggtcgatag ttgtctatgc ttcattgttt tttacctcca atgttctaat
    48301 agaatcaaga aatgttagta cagagatatg atttatttaa gatatcacta tcataaatat
    48361 atagctaaat gaaaaaagtt gcgaatatgg tcttatagaa atagtaatta ttttcagttc
    48421 tcagcggtct tattgattcc gttttattga ttttaaatgt tattcagtga ccctttaaca
    48481 ggtcaatttt tatgtttttg tcttcgtatg actcagtttg atatctagtg atatagtgat
    48541 ttgagtctac atcgaacaat ggaataatcc aataaaaaat gatttagaat atcaaaataa
    48601 caaaaaagag gactacctgt attagtcctc tttttgacca gattttgacc agtctatttt
    48661 acattctaat atattcatat gagctaattt tatgagtaaa tggtaagttt ttccttgttt
    48721 gttttagttt aaactagtgt gtaatttaaa aatctatatg gaagggtctg tggggtaata
    48781 cttaaataaa ggctaggaac ctgataacat caaggttcct agccttttta taataaaatg
    48841 aagcgaaaat aatttctttt aaagagccga gattatcagc tatataagag tgaatttgat
    48901 gcaaaaaaca gagccgaaac agtcttggtg ttcgtagctc tgcttatatg tttcgaattt
    48961 aagacaaaga gttggcaaaa gatgttgata aggaagataa gataactgtg ttatataaac
    49021 taggtaacta actgtaacaa gctaatttta agagattctt attagaagac gtttaggaat
    49081 ataactaaga aaatgattat acaggtaatt gtcatttcaa taatgttttc taattttcgc
    49141 gtctttgttt taagaaaaag gccagaatca gttggaaaaa agttcgtacg atagtgaaga
    49201 ggggcaaaat ttcctactaa tattcccact aacaagagat aaaacggcca ggttggtgtg
    49261 attcctctat aaaaaaatgc ccatgctacc gaaatcccat agattaacaa ctgaacaatt
    49321 tttacaatga aaaaattgtt ctgccattac tcaaagtctc ttcctccttg tatcgtttat
    49381 ttgtttcatt ttatcattat gataggcgct tgaacaatcc attttgtatt ttattgagta
    49441 ctcttggata aactaccagc gattatcata tttatttatg ttactgtaag ccatcatata
    49501 aaagtaaatg agcagatcat agaaatataa aaaagaacta aaattgatct aaattatgtg
    49561 cagcttgact acgtaccaat ctatattact tactagattg gtcgttaatc tattcggatc
    49621 gaatgagcaa aatatttttg tgaatgtcca tccttgatta tgattccaaa aagaatcatt
    49681 gggaggctgt ggcgtaaata ttatactata agccagaaat acctatcatt ctgaactttt
    49741 ttatttagga actttcatct caaggctttt tcaattaatt tgtacccctg ttttttggat
    49801 aagatcatgt gcattgattt tttctatctc tacgttcatc tcctacttaa tgcctatctg
    49861 gctcctgagg gcttcttttt aaggcgtcgt catagtcttg gctgaggatt tcttggtaat
    49921 ctgagggaat ggcttcccaa gtgagcaggc gaacgttcat gacgtgtttt cctttgtgtt
    49981 tgattacagc atggtttttc tctttttcta cctctccagc gtgaaaagac agccatgtac
    50041 catggccaaa gatatcatta catacccaac gttcgatgga attgtccacg tatcctacag
    50101 ggagtgtggt atagccgatt tgtggtttca aaaaaggatt gaatcgatcg atcgtttcat
    50161 ttttgtattg ataattgata agaaaaagat agaccaaaca aaagacgagc agaaagaaag
    50221 atagtgtctt tttgagtgag aagggttgtt ttgatgaata agatttcttg ttaacaagag
    50281 gttcatctgg caatgttggt tctattgatt cggtagctga ttcaatagaa ggaaaatcaa
    50341 tcggcagacc taaagtatga tgtaaaagtg acagcatttc aaggtctggc attgtacgag
    50401 aattttccca actagaaatc gttttatcgg aaacaccaat tttttcgcct aattcttttt
    50461 gcgtaagctt acaatttctg cggtaagttc gtattttttc tccggtgttc atcttcttct
    50521 cctttcgatt ctacagtata tagatttatt ctacatgacc tatacacttg atagatacgt
    50581 ttttttgatg aaaaacggta tgattttatc aaagaatcat ttaagtaagg gagagtggtg
    50641 ataatcaaaa aaaagttcac agttttactc acaatcatag tgaccggtat catgagtggt
    50701 tttgccttgg ttagtataaa taaacaggta ctctatcgga caaatgaatt tttccatcaa
    50761 acagatactt atactccgat tcgattgcca caacatttta ttgggcaaga agaatatgag
    50821 cagctaatca tggtatttga aaatgtctca tcagaaactg agctttctta tatgaaaaaa
    50881 acagtaaatg aatccaatga agtagataca tcttggaact tcaagataca gccaaagctt
    50941 gaaatgacat tgtattatca aggggatgtc aaatcttctc acgagaaggt accctattct
    51001 aatacctcct ttcgcgtaac gtccattgaa gaatcgatca aaataaaaga ttttgaaggt
    51061 gaatttttct tgatgacgag agaaaaagaa gcctatgaac ggttcatttc actattcact
    51121 gatcgattca atcaagcatt tcacactgat tatgccacta atatttttga agactttgaa
    51181 aatagctatt cgtattcggt tatttcaaat acagacgtat ataattatcg agtgtattta
    51241 actattggat tgattctttt attccttttg ttatcatcct ttttcttatt gatgaataaa
    51301 gaaatcagtt tattgttgaa taatgacttt tcattacatc aagtattgtg gtgtttattg
    51361 gggaaaaaga aaatcattat tacattcata attgcaggaa tcattggtgg ttttattttt
    51421 atgtattata aggagcaatt tctttatttg ttccagatgt acgttttctt gttcagcgga
    51481 atatatggta tctctttgat ttctctttta ttagttgaaa aaatcgcttc gttaagagaa
    51541 gcgttcaact atatccaata cattcttttt acatttggat tgttgttttc tcttcttttg
    51601 accgttgcca atgtcgaatt agcaactatc gttttttctt cttcacagtt agctgtttcc
    51661 catgaaccac caaaagaaat cgctggtgag gactatcatg tattttatcc agtaaccatc
    51721 gggaaaaacc acgtggattt tatttattcc aatcgttttg catcagcagc agatcaggaa
    51781 ttatatgaaa ctttgaataa aaacggaagt atattagtca atgtatcaga ttacttgaat
    51841 gaagaaaatg aacagtttgg cagagggata aaaataaatc tgaattatct taagaaattt
    51901 cctttgattg atgtctctgg aaggttgatc caaataaccg aaaaagaaac gcaccctgtt
    51961 atattgatcc ctgaacgata tagacataca gatttaaaaa atgacctggc gcaaataaca
    52021 gaatattatc aagaaatcag tgaaaaagaa ccgaaatttc tatttataaa aaacaatcaa
    52081 cccatttata catttattcc ttctatcccc tggatcgaac attatccggt tgttgaaata
    52141 ctgacattga aaaacagtat gtatttggaa agaaatattc tcagcggaga aatctatcca
    52201 ccgttaaaga tcaagatagg ctctttgtcg aaagaaaggt tatttgagct gattgaagaa
    52261 agtcagttac gtgataactt gcagttatca tttccgtata ctcagactga agagatagct
    52321 gtcaaagtgt taagcagatc atttcattac ttgatacaga tatttttact cactttcttt
    52381 tctttcttct tgttgagcta tgtcatgtta tgcatatatt ttgtctacaa ttgtcggaaa
    52441 atcgctatct ttcgttcaag tggttattcg ctctttgaaa cgtataaaga tttcttcatg
    52501 atgaacctca tcaaatgggg aacgacaagt gtaatcttcc tttttcttat agaaagagag
    52561 cctaaatatc tttttaatat ttttttcttt agcttcattg attttattct ttcagttgtt
    52621 tttattttgt ctacagaaaa gaaaagtcag ttattgctga tgaatggagg ttaacaattg
    52681 gagccgttga tagtgataaa aggtgcgaaa aaaatcattc gaaacaaaac gattcttcat
    52741 ttggatcgtt tcactgcgga tgctggggat cttgtgactg ttgttggaga aagtggagca
    52801 ggtaaaagta cgttattgaa tatattagga ttgaatgatc agttcagttc aggacaattc
    52861 tatttgttcg atcaagagac gaaacaatta actgggagag agaagcgctt gataaaaaga
    52921 aataccatat cttttctttt tcaagatttt gggttgattg aatatgaaac aatcgatttt
    52981 aatttagata ttggattgaa gttttcgaat ttaaatgttt cagataagcg aacagcaaag
    53041 tatgaggcat tgaaaaaagt aaatataaac aagaagctaa gtacgcctat ctacacatta
    53101 tctggtggtg agaagcaaag aatcgcctgt gcgagagtct tattgaagaa tagcaaaatc
    53161 attttagccg atgaaccgac tgggtcattg gataaaagaa ataaggataa agtagttgaa
    53221 atcttatcgc aacaagcttc ccaaggtacg ttagttatcg tagtcacgca tgatccatat
    53281 ctgaccaaaa taggaaacaa gaagatcatg ctttaaaaat caaaaaggaa aggatgtcgt
    53341 tttatgaaaa aagcagttat tatttgtgca ctagggatag gttttgttgg aggaaacgct
    53401 atgtcatctg aagtggaagc aaattcagtt cacggtgaaa tctattacaa atctttaaag
    53461 ccaggagaga ttcattttga tgctaaaggt aatattatta agtcaaattt accagtagac
    53521 cgggaaattt ctagaacagc aattaatcat agctctggaa gatgggttta ttatagtgat
    53581 acaacttgta cgagaaaaac aggtcattct aaccactata gtcgtgtcta taatcgtcac
    53641 ggtgcaaaag cgaaagttgg aaactctatt cgatcagcaa atgcaacaaa aggagtatgg
    53701 gcctatgcaa gtgcgacagg aaatagaact gacacattct catgttggta caatccaacc
    53761 ggttggtatt aagtatccaa aagtttatat aaaatagaaa aaacatataa tgaaacagcg
    53821 ttaaaagtta tttttggtag ttttaatata ttcattttta gttgtataaa aatatatcat
    53881 aataaaaaca acaaaaaaat gtctggaaaa tcgaacataa aagtgtctga tttgaatatc
    53941 ccagcagcgg aagaagaaaa acaaaatcga taacatttgt tctaagaatt agttacgtta
    54001 aatacggaac gcaagataga cttgaaaaac ggtaacaaaa gaagtagtat attaaggtgt
    54061 gatgtgctac ttgacattta ttctgctgag cgaactttct ggattgtgta gccttatatc
    54121 tattcaggac gtatcgagca gattagtaaa ggtgttcttg atgattttca tcttgaacac
    54181 ctttgttttt agcaattcgc aaaaaacgaa aagtaggaga attgataaat gaaaaaaaga
    54241 tttttagcac ttgccatcgt actaggcact ggcttattgt caggctgtac gaatgccggt
    54301 gaaaaaacag ccgttagtta taaaggcggt acaatcagcg aacaagaagt aatggatagc
    54361 ttgaaaaaaa tgcaaggagc agattccgct gtgcaacagt tgatcgttta tcaagtattt
    54421 gaagataaat atggagatga cgtatcaacc aaagaaatcg attctcaata tgatcaaaca
    54481 aagaaacaat tgggtgattc atttgattca caattgaaat ctgcaggtta cacagaacaa
    54541 acattcaaag acagtatcaa acaaagtcta gcctttcaag aaggtctaaa gaaacatatc
    54601 aaattgaccg atgaagattt aaaaacggct tgggaatctt tccatccaga agtggaagct
    54661 caaattatcc aagtagcaag tgaagatgat gcaaaagatg tgaagaaagc tgctgataag
    54721 ggagacgatt tctctaaatt agccaaagac aagtcaactg atactacgac aaaagaagat
    54781 ggcggaaaag tgaaatttga ttccactact acaacggttc cggcagaagt caaagaagca
    54841 gcctttaagt tgaaagatgg tcaagtttct gatgtgatca cttctaccaa tgcttcaact
    54901 tatactacgg aatattatgt ggttaaaatg gtgaaaaacc aaaacaaagg caacgacatg
    54961 gataaataca aaaaagaatt gaaagaaatt gcaacagaca caaaattaag tgacagtacc
    55021 ttccagaata aagtaattgg cgaagtattg aaagacgcta atgtaaaaat caaagacaaa
    55081 gactttgaaa atgttttgag tacattcact tcagattcta gtacagcaag tagtaaataa
    55141 gcggtataaa taaaaaatat gaactattta aatcaataaa actgttcatc taaatctttt
    55201 tattagaact aaatcaacag tagtttaatt actacttgat ttagttcttt ttattttaaa
    55261 aatttttttt acttagaatc cagcgaccct agatattttt ctgtataatc ttaaataaag
    55321 aaaacaatat caaaaaatgc acgtagaagt agataaatgg agtggttttg caaatttctt
    55381 ttatttctag aagttattca cgtatacgga tttgtttttc tcacaaataa aaaggcagga
    55441 agtacaatgg atcatttgta tataaaatat attcatcaat tttccgtttt ggtgttaggc
    55501 acactaatta tagcggggat tgtcgcattt attattcaag gccgtgacat attaacagta
    55561 catcaagagt ttttcagtta tgcatttggt attcctctga tcgctataag gtctgataac
    55621 aaaaagtgga agaaacgcta ttacattctc ttgctatttt tgtgttttta tctgccacct
    55681 aagattttta ctttattagg tatacaatgc ctatcatttt cttttatttc ctatatcatt
    55741 gttttaattt tcctcagttt catagtatgg atgttttatc ttgtcaaaaa ataaatgaat
    55801 gtttcatgaa cagtacttca tcacagccta atttttttct ttatgctatc gttatattaa
    55861 agaaatcaat cagaagggaa agaaagtgat gaaaaaagtt ttattagtgg taaaccccag
    55921 ttcaggcggt gaacaagcaa aggaatttga gcaattagcg atagcaaaat tggaatcggt
    55981 gtttgatgaa gtggtcgtct tgcatacaaa aaaagcaggg gatgcaaaaa actttactcg
    56041 cgaagcggct acggaagggt atcatagtgt gtttgtcatg ggcggagatg gaacagtcaa
    56101 cgaaggaatc agtggaatcg ctgaacaaga acatcggcca aatttcggat ttttcccatt
    56161 gggtacggtg aatgacttgg ctcgagcatt agggataccg ctagaacctg aggaagcgat
    56221 caatcatttt tcgattgaat cagtcaagcc attggatatt ggaaaaataa atgacgacta
    56281 ttttatgaat gtcgtcgcaa ttggctctat ccctgaatca atcaatgatg tagatgcgga
    56341 gaagaagacg aaatttggaa aactagctta tttcatgtca ggaatcaaac agttagcttc
    56401 cacacaatcc tactcgtttc acgtagaagt tgatggaaag aaggaagaaa ttgagagcag
    56461 taccttgctg ataggactaa ctaactcagt cggcggtttt gaaacattac ttccaaatgc
    56521 aaaagtggat gatggtaagc tccatttggt ttatttaaaa gattcttctt tgatcgatac
    56581 gcttaaaaca ttgccagacc tattaaaagg agtagatgaa tctacgaaaa atctagccta
    56641 ccttacttgt gagcaattag ttgtgtcatt agctgatgag gaagaattga cgacgaatgt
    56701 tgatggagat gaaggggata aattacctgt gacaatcaaa gtattgccct cacatttaca
    56761 ggtttattgt taaaatggag gttgtgaccg aggagttggc gggagaacac cgcttgttcg
    56821 ggataaggga ctgtgacaag acttttgtca cagccttttt tttgtacaaa aaagaaattt
    56881 acacctaata gaaaaatttt tccataaaaa tacatatttt aaatatgaaa gtacaattgg
    56941 aaatagatta aaacgataaa ataaaattgt caaaacgtca gagaggatgg tggagaatga
    57001 atcaatgtgt aaaaaagctg attgcgtcga ttttgtttgt cttccctctc ttgatggtga
    57061 catccgtttt tgccgatgta gagaaaacaa acgaatcaac gcaatcagag aatcaaagtt
    57121 ttgacaagac tactttttca gaagaatcga ttacttctga aactacagaa tctcaaagtg
    57181 aggcttctac taataaaagg caagaagaaa ataagacaag tgagacctca gaagtagctg
    57241 aacaaacgtc tgatagcagt gaagcggcac tggcaagagc tgtttcagcg gatgggtaca
    57301 gcgaagctgc cacaacagaa gaactggcgc agcttctcgc agacgaatca gtaactaaga
    57361 tccgactgat tcagcctttg actttagaca gggaattaga gatcaaacga gatatcgtta
    57421 ttgattttgg tggattcgct cacaatttcg gcacacacca tatttatatc aatgagatac
    57481 ctcagtcgat cgagttccag aattttaaag gaacagctgc acatccaggt ggattgacac
    57541 caaatatcga tggaaatgcc attatcattg cttatatgac cgatttttgg ggaagcagat
    57601 atcattttac gggagaaata aaatttaccg ggacacttga tttgtatgat gggtccaaat
    57661 taggactgat ttacgctcct cgtgcaactg tcacattaga tggtgtttcc ggtgtgttgg
    57721 atgtccaacc agtgaaggaa ggactgaatg ccgctccggg taaagcctat ttttgtagaa
    57781 gctatcaatt gaatgtgatc aatggtagtc aactttatgg accgtatcta gggaaattct
    57841 acggttttct agattcagga gatgagactg gtagtggaaa aacagcgcca ggaatccata
    57901 tcttaagcgg ttcaaaggtc tctcttgatt atgatcgggc agatggcgtt agcgatgaag
    57961 gagaggcagt cgatacgcta cctggtaatg tcatgtttga agtcagtggt tcgggaagcg
    58021 aattttcggt aaatacaaag atcaatatta ccaatgacaa caatcgcgga atcatccaaa
    58081 tgagaggttc aggatcgaaa gtcactgtat cgaatggtgg gaaaatcacc atcaatacag
    58141 caactacggg cggatttcgc ttgcaaggag attctagtca aatccatgta tcaagtggtg
    58201 gacaaatctc ggtcaaaatg gctggagatg acgatcaacc cgggaacaat gggatgcgtt
    58261 ttgtaggaaa aggcctttct ttgatcgtag aaggagccgg atcagttatc gacatcgata
    58321 agcagagtgg gcgttccagc gcagttcgtt ttgagaatgg tacgcaaaag ctgacagtaa
    58381 ccaatggagg ctcattgagg gtaagaaata caggtgatgg caaatcttac gtgaacagac
    58441 tcaatcaagg aaatcaagcg atccaatttt atgacgcttc tggttttcta gaaagtccag
    58501 gttcagctga ttttacagtc actggagaaa aatcaaatat cgatatacgc gcagattccg
    58561 gagcaactgt agatacaact ggagatattg atttaaattt tacggcaggt gaaaaaactt
    58621 atttagtcat ggtcgggaaa acaggggaag ataagaccgg catcatcagt ggggatgtgt
    58681 tgaatgtgga cttagaatct cctacctatt ttgatttcca aaacaaacgg attggaagcg
    58741 agaccaatgg cataggcggc tgggtattcc aaggaaatgg agattcttct ttaaagatcg
    58801 cccattcaga aatcgcttta tggcgaaaaa caggcgcaac attcgataac tttgaaaaag
    58861 acccggatta tttttctgat atgacagact tatcgatcaa tgggaccgac ttaggcaatt
    58921 tcgtatcaag ccctagtgca ggtttaacaa atgaatttgc tcagacaggt gccggaatga
    58981 aaaattataa tcgaatcagt gccaataatc agctcccgat gattgaatcc ttaagagtgc
    59041 caacgaatgc agataaaaaa atctatgggc atgtcgttgt tcccgaaggt gtcgaggcaa
    59101 agccaagaga tgcttggaca ggcgaagtag aagtgacttt acgtattact tacgcagata
    59161 cgacgaaatc atctgtagaa attactgctc tcacgcaagg acaaacagat gaaaatgaag
    59221 gggcaggata taacccatgg ggagaaggcg aacaaggcgg cttgttcgaa gttcaagtac
    59281 ccaatgatga gagattaaaa acggaggatc aagtgcaagt tgttgctgct aaaaaagtaa
    59341 gaggtggcca agaagtgaca gaactggact ctgccaaaac gacagtcgat gtgattcctc
    59401 ctgaacctgc taatttaagt accacattga tcaatgcagc ttctcgcacg atcacaggta
    59461 caggtgaagc aggagccttg gtttcattga aaaaaaatca agctgttttg tcagaaacaa
    59521 ctattgatga tacaggggaa tttgttcttt ctatcccaga aaatcaactt tttgcaggag
    59581 atgtattaga aattgtatta aacgaccaag caggagaagc tactgctaag ggtgtgcaga
    59641 acaagccaag taccaacgat gaaattggga atcacaatcc aagtgatacg ccagttgttt
    59701 atcatgatgc cccgcctttt gcacctgcta cgaagatcgt agtagaaggt ggtctctcat
    59761 ttcttgcccc ttccaaactc gattttggtc agatcaaagc aactggtttg acacaagaag
    59821 catttggcca aatggatata caggaaaaat tatggattta tgatcagcga gaaacgaaaa
    59881 atccgtggat cttaacgata aaattatctc agcctttcac aaatgatgca ggattttcta
    59941 tgcaggatgt cctatatttc aagaaaggag aggagtatca attagtgaat gaagaagaac
    60001 tggaaattgt cagaggacaa aattcaacga atgtgcaagc agattattca gaatatttaa
    60061 ataacggtcg ggcgtttaaa cttgtcttaa ccaaagaaaa tcagatagct ggtaattact
    60121 cagcagagat aacatggaca ttagcagatg cgccgacaga ataaagatca aggaggatga
    60181 ttgatgaatc gaatatggaa taaaagtcat cgaaacaact gctctttact tatgatcctt
    60241 ctccttggat tcatcctttg gcagacacct gtccatgcct ccgaattcaa ttttgcggta
    60301 gattttgaat taccggctaa tcagattgat aagaacaaaa catatataaa tgtaaggatg
    60361 gagcctggga aaacacagga aatcccctac acggtcttta accgaacaga taaatcagta
    60421 acagttgaga cattgatcca ctccgctacg acgaattcaa acggtgtgat cgaatatgga
    60481 gaatcaaaaa gtaaatttga tgaaacatta agttatgata tgaaaaactt agcaaaaaca
    60541 gtgggagaaa taactgttcc tcctcaaagt gcggtcactg agacactttc actgaaagcg
    60601 ccagaaaaag agctggctgg tgtggtcgca ggagggatca cgtttcaaga aaaaaaagag
    60661 aacgtttcta acaaaagtac agttggtgtc gtcaatcagt tttctcaagt gaaagcaatc
    60721 ttgatttgaa atagcgaaaa agagtcttcc ccagatttga aattaaaaaa agcttatgcg
    60781 gatcaaagcg atggaagaaa tgtgattaag gcgaatcttc aaaatccaaa tgcttcttat
    60841 atgttcggct tgactacatc ggttgaaatc tacaaaaaag aaacaaaaga gccttacctt
    60901 tcaataaaaa aagaaaaaat agatgtagca cccaatacaa attttgattt acttgtccca
    60961 ttagaaggaa aaaaattagc tccgggacat tatcaagcgg ttgtgaaggg tgcttggaaa
    61021 gaaaaacgct ggaatctcca taccgatttt gaaataactg atgatgcagc ggaccaattg
    61081 aatgaaaagg atattgattt aaaaaatcag ccagaaacat ttgactggcg atggctgata
    61141 cttgttggag tcattcttat tggcttattt gctggattat ttattttaaa agaaagaagg
    61201 aaaaagaaat gaaaaaaaca attgcattaa gtaccgttct agctggaagt ctattattat
    61261 gtacacaaag tgtttttgct gagaataaga gctcagccac tacagaagga acgatttctg
    61321 tgaccaaacc agatgctgca aatcctgaag acgttgttcc agttcctcct gttgatccaa
    61381 atgatcctga tactcctttt acacctgacg aaccaaatca gggaacatcc ggattactga
    61441 cgattgacca agctccagat cttgattttg gaacaatcaa gttaggtaca gcaaaaacag
    61501 tatatgctgc attacaaagc ggaacggaca gtactggagc acaaacacaa gtacagaatt
    61561 tcgtacaagt aacggataaa agcggaaatt atgccggttg gaaattatca gtcaaacgaa
    61621 cagaatttat tggtacaaca tctgatacga ataaactaac tggttcaaag gtgaaattca
    61681 aaaacgccgt gattcgaaat agcgcaacta atagcagtgg aatgccatct accgtttctg
    61741 gaactggcgc tgaaggaatc gaaatcccag cagatcaggc agtagatcta gtaactgcag
    61801 gagcaaatga agggattggc acatggattt attcattagg agagaatgtg gaacaaggga
    61861 gacaatcagt ggaattattt gttcctgtat ccaattatgc tgtagacaac tacgtttcaa
    61921 cattcacatg gtcgttaaca gatgctccta cagaagagga agcgtaaaat catatcaacg
    61981 atttcaggga gtggagccga ttcaaggccc actccctgaa atctgctatg cttacgtctc
    62041 agctcttatt catcaactcg aagcagtttt ttggaagtca tggaaacgtt cctttgtctt
    62101 ttcaaagatc tttcgaccaa cgagtaagga gtcaccgttg aaaaattcta acacataatg
    62161 agcagaatca attttctgca attgcgataa gttgatacag taagatttta aaagtaaaaa
    62221 gtaagcatac ttttctaact ctgtcttcaa ttgttttaaa gaagtattca ctaatttatc
    62281 ttctgtagtt gttttgacca taacttggtt tttcaaacct ttgatacttt caatatataa
    62341 aatttcccga ggatacagga cgagacttcc acctttttga tcaataaaga ttttatcgac
    62401 atttacgatc tgctgttctt tccaattgat ctgttctagc acttgacgaa tttgttcaga
    62461 taaattcctg tcttttctga tatatccaat cggaaggatt ttccgattga tgatctctac
    62521 tgcaaaattc gtataagcgg tgaaaaaaac aatattacta tgtgcatttt ttttacgtat
    62581 ttgttcggcg acatcaatac ctgtgagtga ttgcttcaag tcgatatcga taaagaaaac
    62641 atcgtgatta tcaatagtgc ccaactggat caactgattg atttgagaga tagaagtgag
    62701 ctgcacgata ttctcgatat gatgattgac caattctctg atgatccatt cgctgtgaaa
    62761 ctggttatct tctatgataa aatacttttt catatgcagg ctcctattaa aaaaatatgt
    62821 gtacattttg aaacaaaaca atggaggaat aagatggtcg caacagtgac actttatttg
    62881 acctttactt tgattttaga tttaggtatc tatttattct tttcgaaaca actgctagta
    62941 aaaaacagct ttggttgtat agctttgatg gtggtgacac ttttacttcc tatccctaaa
    63001 gtgagctttc cttacctagt tggcttatta ttgatgtttc ttttttattt tactacgaaa
    63061 aacctgttta tcagctgttt gatcagtaat cttttttttg ctatcactgg actcagcttt
    63121 tttctggttt ttgatctgat ggattattta ggaaagagac atcttggaac agcaagtttt
    63181 tttgcactac ttatcgcatt tattttctat ctattagtaa aaagtataga caaacagacg
    63241 agatttctca acttgctttt tacttatcca actgaacact attttggagg tatttgtatt
    63301 ttattaagct ggggcgtgat ttacttggta ttgggaagtg ttcacttccg ctcatctaat
    63361 tatctgcttc tatctctttt gtcgatagtc atcagttttt tttctttgtt tttttccatt
    63421 atgttgatca gtttccaagt aaaagaaaga caacatcagg aatcgcttca actgtatcaa
    63481 ataaatgaag aatattacca tcatctagag gaattcaaac atgattataa aagtctactg
    63541 ttttcattaa aaattactct tcaggaaaat aaaaaagaaa cgttggaatt tttagaaata
    63601 ttagaagatc attctgcagc tatttttgaa aaaccgcaaa tacaacagct aacaaatatt
    63661 gtatccccag ctgtcagggg tgtcttttta aaatttctag aaaaagcaga atcggagaaa
    63721 atcccgatcc aagtagttat tcctgtcgaa gtgaaaacaa tcccgattga tctggttgtt
    63781 tttattcgct gtttatctat ttttatttct aacgcattcg atcatcgtgt agccgatcag
    63841 agtccgatta cgatcaatct gctggaagaa aaagagggat cacgttttag tatagcgaac
    63901 aaggcaaatc caacgaattt gacgctatcg gagatggtca aaagaggaaa aacctctaaa
    63961 aaaaatggcg ggttaggtct atacagtgcg aaaaaaatgt tggatgttta cgaaaatgca
    64021 gaactaaaaa tcgaattttc aaaacagaat cactattttt ttgtagaaat gtatctggca
    64081 tctagaaaga gcaaatcagc ctactgactt actgtcaaag gataagtttg aggaaagtct
    64141 acacggagat agagacaaca acacagcata ttcaaaagaa acagaagcgt gatgaccgtt
    64201 gtcacccttc tgtttctttt gctatgtagt taaacaaagt agttgacact tccgctactt
    64261 agtattagta cgaaagtata cttcatgata ctaagtagat aagaagtgaa aagcggaacc
    64321 cacgatcgtc aaaatgctaa ctgctttgct tacaccagat aaagggaaga gcaatatcgc
    64381 ttattttagg cgtggagaag caagcaaatc cctacgatca caaaaaaaat gctgacccag
    64441 cagttaagag agttagaaca agatgaaagt atccatcgaa aagcctataa ccaagtcccg
    64501 cctaaagtag agtactcgct caccgaagaa ggaaaatcat taagagagat tctagtagcg
    64561 ttgtctgttt gggaagaaga acgtatcgaa aaacaaaaag cctcaaggga agaagacacg
    64621 ctactgcata cacatggtgg ctacctaaat tattgatttt ctatgacatt tattttaaat
    64681 aaaagaaaac aggagttttt ttaaaagcat catctatttt ttatgtaaaa aaatggtatg
    64741 attaatcaag taagaagtga ttggaagaag gcaagcaaat gaatagtatg gaaaaagtca
    64801 accagtttag agatgaacgt aattggcgac aatttcataa cgaaaaagat ttggctatct
    64861 ctatttcttt ggaagcatct gaattgttag aactttttca atggaaaacg ccggaagaag
    64921 taaaagaaac acaattagaa cgaatcaaag aagaactggc agatgtattg atctattcat
    64981 acatgatggc agacaacctt cacctggatc tagatgagat cattgaagaa aaattgatca
    65041 aaaataattt gaaatatcct gtagaaaaaa gcgcaggtgt acgaaaaaaa tacacagagt
    65101 tgtagaaatg tgtcattcgg ctgttggaaa gagcaaaaga aaaacagtaa aatgagtctg
    65161 gagggagaga aaatgaaaaa aaaaggaaaa gcaaaaagat ggctagtcaa tatcctcttg
    65221 ttcctcttgt tattagtagg actagctttg attttcaatg aacagatcaa ggattatttt
    65281 gtcagagaga caggagataa atatgctata gcgaatgtaa caaaagaaga tctgaaaaag
    65341 aataacgata aagatgtcag ttttgacttt gatgcggtag aaccaatgac gacagaaggg
    65401 gtcatgaggt cgcaaatgag aggaacagat ctaccagtga ttgctagtat cgcagtccct
    65461 tcagtttcaa tcaatttacc ggttttcaaa ggcttggata atacgtcact actttacgga
    65521 gcaggaacat tatctccaga tcaagaaatg ggaaaaggaa attatgcctt agcaagccat
    65581 cgtgcaacga accctgaatt gctgtttaca ccgctagaaa atttagagat gggtgccaag
    65641 atctatttga cagatttaga aaatgtgtat acatacaaaa cattttttaa agaaaaagtt
    65701 gctcctacag atacccaatt actgaatgaa gtcgagggaa aagaaattgt cacactaatt
    65761 acttgtggcg acatggacgc cgtcacgcga ctagttgtac aaggagaact ggaaagtgtt
    65821 acatcgataa aagatgcgac agatgatatg agatctgctt tcaatctaga gacaaaaaca
    65881 ttttaggata ctgggacata agtcgttaca gattaaaaac agccaaagga tatgaatcga
    65941 taaggaaaaa ccaatttgct gaaaaaaaga gaaacgcaaa gaggtgcatc tctgatttgg
    66001 ttgttttcta gatagacttg tgttccagtt tttttatttt ttatatgaaa aggcttgcat
    66061 aaataaaaat tcgatggtat gatgattttg aataagaaat ggattgttac tgtacggcag
    66121 gcaaaaccag aattcagcta agaaacagta ggcgaattct aggttttttt atttttcttg
    66181 agagggcgaa gcaaaatgaa aattgataaa attttgaata acaatgtcgt catttcgaaa
    66241 aatggattcg gggaagaagt cgtttgtatg ggaaaaggac tagcttttca aaaaaagatt
    66301 ggggacgaga tttcgccaga agcggtccaa aaagaatttg ttttaagaga ttcttttgct
    66361 aaaaaccaat tccagcagct gatggctgat attccggcag aggagatcga atgggtcaaa
    66421 caagtagtcg aactggctga agataaacta aaagttgaat tttcgcctaa tatttatctg
    66481 acattgaccg atcatcttca ttatgcgatc acacgagcaa aagaaaatat ccaactgcct
    66541 aatccattat tatttgaaac aaaaaaattt tatccgaaag aatatcaagc agcgaaagag
    66601 gctcttttgt tcgtgaaaca aaagacgggt gtggaacttt ctgaggatga agccggtttt
    66661 attgcctttc attttgtgaa tagccaacaa ggaaacgaag acatgcaagt gacgatgacc
    66721 gctaccacaa tggtaagaga tatactgagt atcatcagta aattcttcgg catcgtgttc
    66781 gatgaagaat ctttgaatta tcagcgtatc attactcatc tgcagttttt tgcccagcgt
    66841 tatctcaaag gggaaggatc tgacgagcaa gatgaatttc tctacgcatt agttcaagga
    66901 aagtatccaa aagcgtttca ttgtgtcgaa cggatcaatg agtatcttcg acaaacgcaa
    66961 aaacaggaaa tggggatcgc agagcagatt tatttaacga ttcatattca aagagtagta
    67021 agtgagaaaa aaagcatcca atcataagga ttgttactgt atggcaggca aaacctggat
    67081 tgaaaaaata ggttcacgaa tgtttgtgac tgtttttttc aatctgggtt tttatttttt
    67141 aaataaaaaa gaaagaagga aaaaacaatg gataatcaag cagttggtcg tcgtgtatgg
    67201 gaagcggtag gaggacaaaa gaatgtcaaa agtttagtac attgtgctac gcggttgcga
    67261 ttcagattaa aagatgaatc tttagcagat acgcaaaagt taaaagaaga cccggacgtg
    67321 atccaagtcg tacaaagtgg cggtcagtat caagtagtga ttggcagtaa tgtagcagat
    67381 gtttatcaat cgatcgtgga tgaggaaggt cttgcagttt cggaggatgg aaaagaagag
    67441 tccaaaaata ttttgaatcg tctgatcgat attatctcta gtatatttac accttttcta
    67501 ggggcgatgg cagccgccgg ggtattgaaa ggattcttat cattggcaac ggtgatgggc
    67561 tggctgacag ctgatagcgg tacttatcaa atattgtttg ctgcagcaga tggtgtcttt
    67621 accttcttac ctgtcatgct tgcttttaca gcagctaaaa agttcaaagc caaccagttt
    67681 ttagcagtag caattgctat ggcacttgtg tatccagcta tcacagcagt tgctggtgcg
    67741 ggagaagcta tcagtttctt tgggatccca gtgattcttt caccatctgg ttatacttct
    67801 tctgtcatac caatcattct agccgtatgg gttcaaagta aattagagcc attcgtaaaa
    67861 aaagtgattc cgcaatttct gcaaatgatt ttagttccgc ttgtcgtttt agtagtgatg
    67921 gttccattaa catttcttgc tttaggacca atcgggacgg ttgccggtaa tgctttaggc
    67981 ggtctcttca actcgattta tggatttagt ccaatcgtag caggtctaat catgggaagt
    68041 ttatggcaag tattcgtgat gttcggcatg cattggggat tcgtaccgat catgtttttg
    68101 aatatcgaac aatatgggtt tgatgtattg atgccaatgc ttcttccagc catcttggca
    68161 caaggtgggg ctgctttagc tgtagcattg cgaacaaaag atacgaaact tcgagcatta
    68221 gggatttctt ctacagtgac ttcgttattt ggaatcactg aaccaactgt gtatggtgtg
    68281 acattacctt tgaagaaacc attcattgcc gcttgtattt ctggaggtat tggtggagct
    68341 attatcggat tttccggagt aaaagctttc tccagcagtt tggtcagctt attgacgatt
    68401 ccgactttca ttaatacggt ggatggtgta gagtcaaatg tgacagtagc agtaatagct
    68461 acaggaattg cttttgttct tgcattcgta ggtacattga ttttaggatt tgacgaacaa
    68521 acacaagaca atcaattaga aaacaaacat gcgaatgcag gagaaccgat tacttcagca
    68581 cggcatacct taaaaagtcc tttaacgggt aaagtactgc cattgagtga agtaccagac
    68641 caagtttttt cttctggcgt gatgggaaaa ggtatcgcta ttgatccaga agtaggagaa
    68701 ctagttgcac ctgcagacgg agagatcacg actatttttc caactggaca tgctgtaggg
    68761 atcacaacga ctgacggagc ggagattttg attcatatcg ggatggatac agtagaatta
    68821 aatgggaatg gctttgagat cttagtcaaa caaggggatc tcgtcaaagc aggagattta
    68881 ttgattcgtt ttgacatcga agcaattaga gcagctggct atagcgtaat cactccagtt
    68941 gtcattacga atactgatgc atttgccgat atacttgaat tagaccaaaa agagattatt
    69001 gcaaacgaag atgttctggc aattgtaaaa taaggaggaa atcaggatgc aatcacgttt
    69061 ttcagaaaaa tttttatggg gtggagcaac tgctgccaaa tcaattagaa ggggcttatg
    69121 atcgagacgg aaaaggacta tccgttgcag atgctatgcc tggaggtaag cagcgctttg
    69181 cgattatcgg cagtgaagaa tttgactgga cgattgatca agaaaaatat atttacccga
    69241 atcatcgagg aatcgatcat tacgatcgtt tcaaagaaga tcttgccttg tttgcggaaa
    69301 tgggattcaa atgttatcgt ttttcgattg cctggacacg aatttttcca aatggagacg
    69361 aaggtactcc taacgaagcg ggattagaat tttatgatca gctgattgat gagtgcttaa
    69421 aatatgatat tgaacctgtg atcacgattt ctcattacga aatgcctctt catttggcaa
    69481 aagaatacgg tggctggaaa aatcgaaaac tgatcgactt ctatgaacga tttgctcaga
    69541 cagtattaga gagatacagc agtaaagtca aatattggat gactttcaat gaaatcaatt
    69601 cggcctttca ctttccagct ttaagtcaag gattagtaaa aagcaatgga gcaggagagt
    69661 accaaaatat tttccaagca tggcataatc aattcgttgc aagtagcaaa gccgtaaaaa
    69721 tcggccacga attgcgttcg gatatccaaa tcggctgcat gattatctat gcaacaactt
    69781 acagcatcga tgccaatcca gtcaatcagg cagcaacaat gatccaaaac caagaattca
    69841 attttttctg cacggatgtc caagtacgtg gagaataccc tgcatacaca gcgcgtaccc
    69901 ataaaaaata tggagtcgat ccggaaaaat tagagcaaac ggaaaaagac ttccagttgc
    69961 taaaagaata tccagtggac tacatcggat ttagttatta tatgtctacg gcaatcaatg
    70021 agactgatcc acaagcagca acttcagaag ggaacttgct gggaggagta aaaaatccgt
    70081 ttcttgaggc aagtgaatgg ggctggcaga tcgatccaga agggctgaga atcgcattga
    70141 atgaattata caatcgttac cagaaaccat tattcattgt tgaaaacggt cttggagcca
    70201 ttgatcatat ggaagaggat ggcacgatta ttgatgatta tcggatcgat tatttacgtc
    70261 gtcatatcga agcgatggct aatgcagtag cagacggtgt agatttgatg ggctatacgc
    70321 cttggggatg tatcgatctt gtcagtgcat caacaggaga aatgagtaaa cgttacggct
    70381 ttatctatgt ggatctagac gatgaaggaa atgggacact gaatcgatca aagaaaaaat
    70441 cttttgattg gtataaagaa gtaattgcct caaatggcgt aaacttgtaa ttaaagcagc
    70501 tgaatgaaaa agaacacact tctcacatgt gatttgtgtt ctttttttat tttttatgtg
    70561 ctattctttt agtaatagta aagcaaaggg tgacgttatg aaaaggacac gtgtactata
    70621 cttggaagtc gcagataaaa ttaaagaaga tattttctca ggaaaatatc cagtcggttc
    70681 gatgttgccg acagaaagtg aattagaaga actgtttcaa gtcagcaaaa tcactgtccg
    70741 aaaggcaatc gaactgctgg catcagatga atatgtcgca aagaaaagtg gtcgtgggac
    70801 gacggttcta agcaatcgtc cttacaatcg attatccaaa gcggcaagct ttacgcaaat
    70861 cttggagaac tcccgatatg aagtccggaa agaaacgctg gatttgcaat tggtccatat
    70921 aaagaaggaa gatcctttgt tttccttttt cggtgagcaa gctgtttgtt ttaagcgctt
    70981 atattatttg aacaatcagc cttatattta ttttgttcat tatcttccac aagaaatgat
    71041 gcaattcagc agagaagaat ttggcaaaga atctttgtat cgtttgctga cgaaaaaagg
    71101 atatactatt gactcatttt ctgatgattt ctcggcagta tttttaacag aagaagaaca
    71161 aggaattctg aaaacagacg cacagatcgg catcaaacgg atacggaaat cgtctacgca
    71221 aattggggaa atgattgaat attcggaggc ggtctaccat acggagctgc atccttacca
    71281 tattgattat gaaacataaa taaaaaaaga ggttgtgaaa aagctgccct gcatcaagta
    71341 ataagaacag ccaaacaaaa atagcttctc atatttttcg tttggttagg cttattaccg
    71401 cagatacacg gctgtttctg gattaccatt tattctggat aagggggtgt ggcacgattt
    71461 tcagaaggaa tctatgaacc aatcactgta ttgattttag cttcgatggt aaccccattc
    71521 ggcttgacga tcgcttattt cttagggaaa atcattcgta aaaatatact caatcgtcaa
    71581 gaaatcgata cattgaagac cgcttttcct atggggatct gtcagatcac agagggatgt
    71641 ttccctatcg tattgaacga cttggcacgc aatgtcatag caacgggagt cggtgggaca
    71701 gtaggtggcg gattaagcat gttttggggc gcagatagcc gtattccggc atcgggaatg
    71761 tttgcagtcg ctacgatgac cagaccatgg gcattcattg gtgcattgtt agctggttct
    71821 tttgtcacag gaataaccat tttattattg aaaaaaccag tagatccgaa tgcagaaatc
    71881 atccaagaag aaaaagagga agaagacata tcttgggatg atttgacaat tagttaggag
    71941 aggcagaatg gattacaaga aaaaagtaca atagatgtac cttgagacag gaatggtatg
    72001 aaagaatcag agttagaaag aatcgaatat acagattttg gtttaggagc gatcgaaact
    72061 gtccagagca tcggcaccct ccttgaaatg acgatgaagg aaaaatgaga acctttcgtt
    72121 gtcgaaaggg tctggtatac cttatgtcga aggaaaggaa acaaaaaaac tttcctgtac
    72181 tattccaaaa aggaaagaaa gttattatac agtaaaaaaa gaaattattc ttcatgctag
    72241 agatcagtac acgttcgagc ccaatatcaa acactagttc caggcaggcg caacaggtgc
    72301 ggttatcact gaattttctt ccagcagtga cgattcaagt gatattttta caaatccagc
    72361 tatttatcgt taacaaaaag atttttagga gaagctgatg aacaaaaatg atcaaatttt
    72421 gatcgtttag ttcgtcggct tttttattta gagaaacatc atttatcagt gaaacataac
    72481 acgagaacaa aatgattttt tcgttaataa aagaaacaag tatttgttgt ttaaaataat
    72541 acacgttatt ggcatatagg acgaaatccc agtatcttct ataagtaagt catttcatat
    72601 cgatgaaaac acgttattta tttttgtttt atctataaaa tggctgaaaa atgatttttt
    72661 tctaaatact atatatagtg gcgtctatcc tatataccac taaaaaacta caaaatctag
    72721 tcttttattt taaaaaaaca cgtgtagaat gaagatagac ttttgagtta gggagtgttt
    72781 ttttatgaaa cttactgttt tttgcggttc ccgttttggt aataaagaaa gttacaaatt
    72841 tattgcgcaa acgttaggaa aatacatggc acaagaagaa atcgaactgg tttacggtgg
    72901 ttcatactcc gggattatgg gagtgattag taaaacagta ctggaaaata acggaaaagt
    72961 cacaggtatc tatccgaatg ggctttttga aactgaattg cctggaaaag atgttactac
    73021 atttattcct acagaaacga tcgatgaaag aaaggtatta ttatttgaaa agggagatgc
    73081 tgtcctcgtt ttccctggag gtctaggtac attggaggaa ttctcacaac ttctttcttg
    73141 gatagcaatc ggtctgacgc ccgacaagcc aatcggaata ttgaatattg gcggttatta
    73201 cagcggcttg caacaattat tagaaacttt tgcaaaagag gattttatgg ataaaaaatg
    73261 gttggatcaa gtaatctttt ccaacaatcc attggaactg gtcaatgaat tacgagcagt
    73321 atctattaaa aatcagttat tattgaagga ggcaaaataa tgagtcaaat atatttagca
    73381 ggaccatttt tttcagagga gcaaatcgat cgagtaagtc gtatcgaaaa agcgttggag
    73441 gaaaacaaaa cagtcacaag tttctactct ccacgtcatc accaagaaag caattatgaa
    73501 ctattcagcg ctggatgggc gcaggaagta tacgaaaaag atatggaaga actgacaaat
    73561 gcagaatttg ttgttgctat ccttgacttt gagcatcaaa caatcgatcc aggaacggct
    73621 tacgaattag gtgtggcaac tatgttgaaa aaaccgatga tcattgtaca agaagagacg
    73681 gtacctacga acttgatgat tacacaaagt ctccatacgt atttaaaatc agaccaagct
    73741 gtacgtgaat atgattttga aacattgcct gttgaaactt acgtaggcga gtacttatag
    73801 aagaggaagg gttgtatgtc agtgattttg atttcaggaa cgattggtgc aggtaagagc
    73861 agtttgacgg atatgcttgc aaaagagatt gattctaaac cattttatga aaatgtcgaa
    73921 gataacgaag tattgccgct cttttattca aatcctgagc agtacgcttt tttattgcaa
    73981 atcttctttt tgaacaagcg atttttagca atgaaaaatg ctttagtgaa cgatgacaat
    74041 gtgctagacc gatccatcta tgaagatagc ctgttgttcc atctaaacgc tgatttaggt
    74101 cgggtaacgg atatcgaagt acagcagtac gatagcttat tagatacgat gctaaacgag
    74161 ttagatgatg ttgctccaaa aaaacggcct gatttgatgg tccatatcaa agtttctttg
    74221 gacacgatgc tagaacgtat caaaaaacgt gggcgtgact atgaacagct agaaagcgat
    74281 gagacgttat atacctatta tgaaacgttg aacacccgct ataatcaatg gtatgaagat
    74341 tttgatgtat gtcctaaaat acaagtggat ggggataaat tcgattttgt agaaaatcca
    74401 gaagatgcaa aaaaggtgat ccgtcagatc caagaaaaat tagctgaatt ggaaggaaag
    74461 cctgctggaa aatattttac ggataaccag catgcaaaag gtcaagaatt aggctaaaaa
//
LOCUS       DANKEF010000008        74111 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000008 DANKEF010000000
VERSION     DANKEF010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 74111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74111
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.008"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(513..869)
                     /locus_tag="PWZ48_000592"
     CDS             complement(513..869)
                     /locus_tag="PWZ48_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HDL2260271.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(883..1293)
                     /gene="spx"
                     /locus_tag="PWZ48_000593"
     CDS             complement(883..1293)
                     /gene="spx"
                     /locus_tag="PWZ48_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2260272.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1639..3825)
                     /locus_tag="PWZ48_000594"
     CDS             complement(1639..3825)
                     /locus_tag="PWZ48_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDL2260273.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4043..4726
                     /locus_tag="PWZ48_000595"
     CDS             4043..4726
                     /locus_tag="PWZ48_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HDL2260274.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            complement(4807..5526)
                     /locus_tag="PWZ48_000596"
     CDS             complement(4807..5526)
                     /locus_tag="PWZ48_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2260275.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTTK"
     gene            complement(5838..6044)
                     /locus_tag="PWZ48_000597"
     CDS             complement(5838..6044)
                     /locus_tag="PWZ48_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260276.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            6289..6891
                     /locus_tag="PWZ48_000598"
     CDS             6289..6891
                     /locus_tag="PWZ48_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HDL2260277.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFVLEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            7032..8312
                     /locus_tag="PWZ48_000599"
     CDS             7032..8312
                     /locus_tag="PWZ48_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2260278.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIGDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            8325..9053
                     /locus_tag="PWZ48_000600"
     CDS             8325..9053
                     /locus_tag="PWZ48_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2260279.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(9122..10645)
                     /locus_tag="PWZ48_000601"
     CDS             complement(9122..10645)
                     /locus_tag="PWZ48_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HDL2260280.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKNGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            10897..12078
                     /locus_tag="PWZ48_000602"
     CDS             10897..12078
                     /locus_tag="PWZ48_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2260281.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(12138..13766)
                     /gene="treC"
                     /locus_tag="PWZ48_000603"
     CDS             complement(12138..13766)
                     /gene="treC"
                     /locus_tag="PWZ48_000603"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDL2260282.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGEKMSEGNGWNALFFNNHDQPRALNRFVAIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(14217..16928)
                     /locus_tag="PWZ48_000604"
     CDS             complement(14217..16928)
                     /locus_tag="PWZ48_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260283.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLSQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(16938..17639)
                     /locus_tag="PWZ48_000605"
     CDS             complement(16938..17639)
                     /locus_tag="PWZ48_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260284.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            17825..18424
                     /locus_tag="PWZ48_000606"
     CDS             17825..18424
                     /locus_tag="PWZ48_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2260285.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTITKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            18505..19518
                     /locus_tag="PWZ48_000607"
     CDS             18505..19518
                     /locus_tag="PWZ48_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2260286.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            19626..20543
                     /gene="rbsK"
                     /locus_tag="PWZ48_000608"
     CDS             19626..20543
                     /gene="rbsK"
                     /locus_tag="PWZ48_000608"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDL2260287.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            20571..20966
                     /gene="rbsD"
                     /locus_tag="PWZ48_000609"
     CDS             20571..20966
                     /gene="rbsD"
                     /locus_tag="PWZ48_000609"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDL2260288.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            20966..22339
                     /locus_tag="PWZ48_000610"
     CDS             20966..22339
                     /locus_tag="PWZ48_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2260289.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            22696..24840
                     /locus_tag="PWZ48_000611"
     CDS             22696..24840
                     /locus_tag="PWZ48_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2260290.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(24892..25932)
                     /locus_tag="PWZ48_000612"
     CDS             complement(24892..25932)
                     /locus_tag="PWZ48_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2260291.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(26209..28011)
                     /locus_tag="PWZ48_000613"
     CDS             complement(26209..28011)
                     /locus_tag="PWZ48_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260292.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIVLGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(28025..28777)
                     /locus_tag="PWZ48_000614"
     CDS             complement(28025..28777)
                     /locus_tag="PWZ48_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260293.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(28786..29148)
                     /locus_tag="PWZ48_000615"
     CDS             complement(28786..29148)
                     /locus_tag="PWZ48_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2260294.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(29324..30196)
                     /locus_tag="PWZ48_000616"
     CDS             complement(29324..30196)
                     /locus_tag="PWZ48_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HDL2260295.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(30247..30735)
                     /locus_tag="PWZ48_000617"
     CDS             complement(30247..30735)
                     /locus_tag="PWZ48_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HDL2260296.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQASGWYITEDKELELEGVMARIRK
                     KN"
     gene            complement(30873..31664)
                     /locus_tag="PWZ48_000618"
     CDS             complement(30873..31664)
                     /locus_tag="PWZ48_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HDL2260297.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(31719..32327)
                     /locus_tag="PWZ48_000619"
     CDS             complement(31719..32327)
                     /locus_tag="PWZ48_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HDL2260298.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(32320..32637)
                     /locus_tag="PWZ48_000620"
     CDS             complement(32320..32637)
                     /locus_tag="PWZ48_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2260299.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(32793..33521)
                     /locus_tag="PWZ48_000621"
     CDS             complement(32793..33521)
                     /locus_tag="PWZ48_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDL2260300.1"
                     /translation="MPTGVLINVGSVLFGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            33680..34561
                     /locus_tag="PWZ48_000622"
     CDS             33680..34561
                     /locus_tag="PWZ48_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2260301.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARSFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(34798..35841)
                     /locus_tag="PWZ48_000623"
     CDS             complement(34798..35841)
                     /locus_tag="PWZ48_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2260302.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFFQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(35844..36512)
                     /locus_tag="PWZ48_000624"
     CDS             complement(35844..36512)
                     /locus_tag="PWZ48_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2260303.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            36654..38990
                     /locus_tag="PWZ48_000625"
     CDS             36654..38990
                     /locus_tag="PWZ48_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260304.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(39105..39416)
                     /locus_tag="PWZ48_000626"
     CDS             complement(39105..39416)
                     /locus_tag="PWZ48_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260305.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            39597..41072
                     /locus_tag="PWZ48_000627"
     CDS             39597..41072
                     /locus_tag="PWZ48_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HDL2260306.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            41119..41703
                     /locus_tag="PWZ48_000628"
     CDS             41119..41703
                     /locus_tag="PWZ48_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDL2260307.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            41705..42124
                     /locus_tag="PWZ48_000629"
     CDS             41705..42124
                     /locus_tag="PWZ48_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HDL2260308.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(42405..42689)
                     /locus_tag="PWZ48_000630"
     CDS             complement(42405..42689)
                     /locus_tag="PWZ48_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HDL2260309.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(43041..43751)
                     /locus_tag="PWZ48_000631"
     CDS             complement(43041..43751)
                     /locus_tag="PWZ48_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HDL2260310.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(43748..45220)
                     /locus_tag="PWZ48_000632"
     CDS             complement(43748..45220)
                     /locus_tag="PWZ48_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2260311.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            45358..45831
                     /locus_tag="PWZ48_000633"
     CDS             45358..45831
                     /locus_tag="PWZ48_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2260312.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(45984..46535)
                     /locus_tag="PWZ48_000634"
     CDS             complement(45984..46535)
                     /locus_tag="PWZ48_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2260313.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            46640..47170
                     /locus_tag="PWZ48_000635"
     CDS             46640..47170
                     /locus_tag="PWZ48_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HDL2260314.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(47653..48096)
                     /locus_tag="PWZ48_000636"
     CDS             complement(47653..48096)
                     /locus_tag="PWZ48_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260315.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(48112..49272)
                     /locus_tag="PWZ48_000637"
     CDS             complement(48112..49272)
                     /locus_tag="PWZ48_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2260316.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            49779..51479
                     /locus_tag="PWZ48_000638"
     CDS             49779..51479
                     /locus_tag="PWZ48_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260317.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(51581..52414)
                     /locus_tag="PWZ48_000639"
     CDS             complement(51581..52414)
                     /locus_tag="PWZ48_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDL2260318.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(52718..54883)
                     /locus_tag="PWZ48_000640"
     CDS             complement(52718..54883)
                     /locus_tag="PWZ48_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2260319.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            55143..57437
                     /locus_tag="PWZ48_000641"
     CDS             55143..57437
                     /locus_tag="PWZ48_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2260320.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            57430..58095
                     /gene="pgmB"
                     /locus_tag="PWZ48_000642"
     CDS             57430..58095
                     /gene="pgmB"
                     /locus_tag="PWZ48_000642"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2260321.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            58088..59095
                     /locus_tag="PWZ48_000643"
     CDS             58088..59095
                     /locus_tag="PWZ48_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2260322.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            59117..60136
                     /locus_tag="PWZ48_000644"
     CDS             59117..60136
                     /locus_tag="PWZ48_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260323.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(60417..61784)
                     /gene="nhaC"
                     /locus_tag="PWZ48_000645"
     CDS             complement(60417..61784)
                     /gene="nhaC"
                     /locus_tag="PWZ48_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HDL2260324.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(61893..63314)
                     /locus_tag="PWZ48_000646"
     CDS             complement(61893..63314)
                     /locus_tag="PWZ48_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2260325.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(63557..65434)
                     /gene="tdc"
                     /locus_tag="PWZ48_000647"
     CDS             complement(63557..65434)
                     /gene="tdc"
                     /locus_tag="PWZ48_000647"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HDL2260326.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(65725..66981)
                     /locus_tag="PWZ48_000648"
     CDS             complement(65725..66981)
                     /locus_tag="PWZ48_000648"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDL2260327.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(67093..67298)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            67561..68862
                     /locus_tag="PWZ48_000649"
     CDS             67561..68862
                     /locus_tag="PWZ48_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDL2260328.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(68931..69530)
                     /locus_tag="PWZ48_000650"
     CDS             complement(68931..69530)
                     /locus_tag="PWZ48_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2260329.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            69784..70665
                     /locus_tag="PWZ48_000651"
     CDS             69784..70665
                     /locus_tag="PWZ48_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260330.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            70678..72288
                     /locus_tag="PWZ48_000652"
     CDS             70678..72288
                     /locus_tag="PWZ48_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HDL2260331.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(72365..73024)
                     /locus_tag="PWZ48_000653"
     CDS             complement(72365..73024)
                     /locus_tag="PWZ48_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2260332.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(73307..73972)
                     /locus_tag="PWZ48_000654"
     CDS             complement(73307..73972)
                     /locus_tag="PWZ48_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HDL2260333.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
BASE COUNT        22124 a        14945 c        13213 g        23829 t
ORIGIN      
        1 cccgattcgt ttttggctgc cgtagcaact tttatatcat atcagagaga caagtaattg
       61 tcaactcttt tttaaaagtt tttttgattt cgtttttcga aatcttacag caactcattt
      121 atcataactt atcatttgtt atttgtcaag aacttttttg aagttttttt cgatagaatc
      181 aagcttcaca agtggttctt ttttaacaac tcagttatct tatcatgtca tttctaacat
      241 gtcaacaact attttatatg ttttttaaaa tactatttta ttgctttgct acttaaaaca
      301 acagaaatga atatatcatg taaatttaca catgtcaata tatttttaac tctatatccc
      361 cacctagatt tgaattttga ccacataaat tgagcatgtc tctatagtag aaaaataaag
      421 tcaaaaaaag aacacataac acgcttgatg ctaagtgttc gactgttaca actgtatgaa
      481 atgctcagtg acttgattaa aacagttaaa gtctatttat ttaagataag ctgttccatc
      541 aactgctcat tgtatccaat aaatagaact tcatcgataa taatcaaagg acgtctgagg
      601 agattggtat gttgtgtgca taaaaaaatc agttgttttt ttcctacaca ggaaaggtca
      661 atcgcaatgt tgtgaaaagc ctcagaccaa gtcgcaaata gctgttctgc ttgcacttct
      721 tcaaaactgc ataattcttc tgcttcttct gtacttagat cgcgttcttc taagtcaaca
      781 aattgataag gcaattgatg gtgttctaac cattgtttcg ccttcagact tgttacacag
      841 tgcgctgatc catagacttt cacttccacg aaggctccct ccctatgaat ttaagatgtt
      901 attgttttgt aaatagatca gatttctggc aacacatcga atttcacgag ggacaaatga
      961 ccggatctca tcttcattaa atccgattag tattcttttt tcatcaatca aaataggtcg
     1021 tctcaataat gaaggatatt ctttgaataa gacgatcatt tgctttaaag ttaattcgtt
     1081 aaaatctatg cccagctttt cgtaaatttt cgaccgagtg gaaataatat cttgaactcc
     1141 tccttcagtc gctgccaaga tctttttgat ctcactttct gttaacggtt cggcaaaaat
     1201 attcttctct tcaaattcaa gttgattcgc ttgcaaccat gctcttgctt ttctacagga
     1261 agtacagcta ggcgaagtat atacttttat cattttctca ctccttttta attataatcc
     1321 aattctacag taatttcttt taaaatacca attattagct aataaaatat aaaaaaaatc
     1381 aaaataactg attatttacg tttgaataat gagaattttt ctatatattc tctattttga
     1441 taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct tgtcacacct
     1501 cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc tgaaatttca
     1561 caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact gaccacttct
     1621 gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt ctttgataaa
     1681 tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat acagacgatt
     1741 tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg tcgttactgg
     1801 ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt tataaggcaa
     1861 acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt tacttttgtg
     1921 aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca agcgggtttt
     1981 taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc tgaaagtagc
     2041 atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt ccttagctat
     2101 acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct ggtctgtttt
     2161 acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat tggggcttat
     2221 cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt attgatcgca
     2281 ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct ggtaggcctg
     2341 atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat agaaatgttg
     2401 gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta caaaaggaat
     2461 attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc ggtataatat
     2521 gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt tgattagcca
     2581 ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg cttctttttg
     2641 tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca atctatttgc
     2701 tccttggaca atagctgccc gagaacgata attagttaat aagaaaagag ttttggtagg
     2761 atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc catagatact
     2821 ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca tccgtttcac
     2881 gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat aatcatattt
     2941 ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat tttggagtac
     3001 atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg tgagcaatcc
     3061 atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat atttttcttt
     3121 aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt ctaaccgaga
     3181 agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac gtatcagctg
     3241 ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt gtattccttg
     3301 cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa agatactatg
     3361 gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc gtttttttag
     3421 ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt ctacttgttt
     3481 ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc tcccagctcc
     3541 tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt gttcttgatt
     3601 gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc cttcactttc
     3661 ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg cttcataggt
     3721 agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact tgttctccca
     3781 gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat tgatttcctt
     3841 cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt tcgttgtcta
     3901 ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat atgattttag
     3961 ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa gacaaaaata
     4021 ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta caataggact
     4081 tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat atggtgcttt
     4141 tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg acggagaatg
     4201 gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga ttcttctaac
     4261 aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct tgtttgttct
     4321 atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg agcatcccgc
     4381 attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt ttgataaaat
     4441 caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg tagcaacgaa
     4501 cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt tccactatct
     4561 aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc atcatctgtt
     4621 tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc tcactcagac
     4681 atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc aatgaatcaa
     4741 aaaaactaag caagatgctg ctcacagaga aatatctgcg agtgagcaat ttgcttagtt
     4801 tttatattat ttagttgtaa agattccctt tgctttcggg ttatttgtag tgtagcggat
     4861 cgactgggca tctgcctctt ctgtcatttt ctttgcccgc atcttgattt cttgtacgat
     4921 ttgttctcca catgccggat cggctacgaa atcatatttg tccccatcga tcactatttt
     4981 gggacatata tcaaactgtt cataccactc ttcgtatcgt cgattgagtt ctttatagta
     5041 ggaatagagt tcaggatctt gttctagctg ttcatattcc cggcctcttt ttttgattct
     5101 cgctaacata gtttcaaagg atacacgaat ataaaccagt agatctggtt tcttttgtgg
     5161 cgaaatttct tctaactcct tcagcatcgt ttctaaaaga ccttcatact gctttacttc
     5221 tacttcagaa acacgcccta aatctgcatt caaatgaaac agcatactat cttcgtagat
     5281 ggagcggtct agtacgttgt catcatggct gaaagcatct ttgattgcca aaaagcgttt
     5341 gttcaaaaag aaaatctgta ataaaaaagt atacttttct ggattagaat aaaatagcgg
     5401 caatacttca ttatcttcca cgttctcata aaaaggtttt gtttctaaat cttgggcaag
     5461 catttcagtt agtgtgcttt tccctgcacc gatcgttcct gcaacaatga tcacaattat
     5521 cggcatcctt cctgtaataa aatctgttct tcattttact acttcattta tagaaataaa
     5581 agccttgact ttatctgctc cttaaaagct acttctctaa aagtagccaa aagtaactcc
     5641 tattcatgaa aataaaggct gtgacaaaag tcttgtcgca gccctttttt caaataaaca
     5701 gtattctcct gccaacacct cggtcacaag gcaatattca taaaacatga aaagctgttt
     5761 tttaacatta ttccttgtaa ctcagagcta aacgggagac tcacaacctc tttatgctct
     5821 caacatttta tctgttttta ttcttctatt gtacttgatt tgcgtacaac taactgccag
     5881 tgcagattat gagcggtatc attgtcttct actgcgcatt tcatgacacg tatcatcaca
     5941 ctattttcat agattttttc tatttttcct tttattggtg attcaaataa atcagattta
     6001 aatgcatatg tttcgccgac ttcaaacaat tgttcatttt ccataagaat ccctccatca
     6061 ccttttataa ggtcagtaat tttttaacat aaatcactcg aaaaagcaca aacttgtcat
     6121 aaaaagtact tttcgtatca aaactgtcac tttttttggt aaggttcttg gaaaacgaag
     6181 atttttttag gagatcatga cggttttgat gtcaaaacca ctttttatga aacaaaagac
     6241 gttttttatt ttttttagca taaaatttgt ggtggaggta cgtatgagat gaaagataaa
     6301 gttgtgaaca aattattaga agccttattc agcggagaca aatcggaaga agatatggtg
     6361 tatgtccaag taaaatttgt cttagaagtt ttattgaata atcttggaaa actagcggtc
     6421 gttctagtct tttctctggt gacaggctca tgggcggaaa caggaatcac ctttctttct
     6481 tatatctgca tccgcagata tgcttatgga ttgcattccg actctgaatt cgtttgtctg
     6541 ctgtggacac tactttattt atggggcgtt cccttggtga tgaaacatct gcaattgact
     6601 atctccttcc caatgatggt ctttttgtta atcagctgct ttttgttact gcttcgctat
     6661 ggatcaagag gtacagcaat caatcccatt gaacccgaga aacgtccccc attattaaag
     6721 aaggcgatca gcatgtttct aattttttct ctcattacgt tatttttctc agcttcttac
     6781 ttttctacct atttgctact tgggattgtt ttagaaatag ctacgttgct tcctattaca
     6841 aactacttga tgaattttgg aggaatcttt catgaaaaaa acaatcatta aacaattaac
     6901 taacttatct gacttttttg tatctggctg ctgctttggt atgagttctg aattcaaaat
     6961 ccctgaaaaa ttaaaaaaat aatacgccaa ttgtgctata atttggtaaa aatcaatggg
     7021 agctgtactt tatgttttta tggtccatag cgttgcaata tttgatttac acaatttgta
     7081 tcttattgat tgaccatcac atccgataca aatatctgtt tgcgggagtc attctgattt
     7141 cttgtatttt gattggatgg gtctattcga ttttagggat ttttactgct ctaattgcta
     7201 taggaatgct gatgcttact tttttcttct tttcgacttc gcgtcaacaa ttggtttacg
     7261 cgcttccttt tgggttattg acgtcgcttt taggagatca cttaacaagc atcggcgatt
     7321 tttttgtttt gaaatcttcg gttattgaac caggagattc attgcagtat tttcacattg
     7381 tcttatccgc ttttttatct gctttattag ctttttgttt aaaaaaatcg ttagagactt
     7441 ggtttcacac tgtggaccgc cgtatgatcg gtacgatcgg gacattgata ttatttacat
     7501 actatatcat tattttttat acccgttttt ctggtgaaac acctgatgtc ttagctttga
     7561 atacgagttt tttcattcta tatctgtgtg ttggcctgat catcttggtt tattattgga
     7621 aaatctctaa taagaaatta gctgatcagt tattagagca acaagtcagg atgcaacagg
     7681 attatatcaa agatctagaa aaaaactatc aagacttgag agaattcaaa catgattacc
     7741 aaaatctgct tttttctctt cacagctata tttcagaagg cgacttagaa ggattacggt
     7801 cttattatga tcaaacgata ttacctacac gccagatgat gaaccttttt ccggccaatc
     7861 taagtatgtt ggataatatg aaagttcctg agatccgtag tttactgtca ttaaaactca
     7921 tgatggcaca ggaaaaaggg ttgatcgtcc agcttcattt tcctgagcca atcgaactgg
     7981 atacgaaaca tacggtaaac cttgtacgta tgcttggtat tatcttggac aatgcgattg
     8041 aaggagctag tgctgcaaag aagaaaaaaa tcgaattgac catactcaag aagaatcatt
     8101 ccgtgatgat tcgtgtgatc aatacaacaa caaatacatt accgttaaac cagttgaaac
     8161 aaaaagggtt ctcaacaaaa acaaatccaa aaaacgaagg ccttggcttg tttatcttgg
     8221 atgaattgac aaagaccaat ccttatcttt tccttgaaac atcaattgaa aaccagcgtt
     8281 tttctcaaac gatttatata caaataaaat aaaggagtaa tgatatgatt cccatttata
     8341 tttgtgaaga cgatccaaaa caattagaag ttttatcaac ggtgatcaaa aatagaatca
     8401 tgattgaaaa cttagacagt tatgtccata tttctacctc tgatccggaa gaacttctca
     8461 atgcagccaa gatcagaact tcttcttttg gtatttactt tttagatatc gaactagaag
     8521 acagtgaaat ggatggtgta gcgatcggta aattgatccg tgaattagat cctttaggaa
     8581 aaataatcta tgtgacttcc catacggaaa tgtcgatgaa aatcttaaaa agcaacatcg
     8641 aaccgactga ttatatcgtc aaagaagatc tttatgattt aaaagaaaat gtcgagcata
     8701 tcctgagcaa aattttcgaa gattttcaaa tgtcccaaat ccaaaaagat atcttcaaaa
     8761 tagagtttaa tgatgagatc aaatttttac cgatcaaaga catccaatat ttttctactt
     8821 cgctaggagc gccacataaa ctggaagtcc atttgactca tgcccaatta caattctatg
     8881 agaagatcaa agaaatcgag aaaatgcatc ggaattttat ccgttgtcat aaatcctatg
     8941 ttatcaatac acaaaatgtg cggtcgatta ataaaaaaac cagagaagtc acgttggcaa
     9001 acggcgagac catcccagcc tctatccgtg gattaaagaa attgatttca taaaaagaac
     9061 cgaatagttt ggaccgacta aatgaccagc cgatccaaac ccttcggttc ttttttgcct
     9121 atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct ggataaaatg
     9181 ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct gcatataaat
     9241 gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata tatctaaaag
     9301 cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt cctgcctcgt
     9361 aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt ccattcttaa
     9421 acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt tgatagccct
     9481 tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt tctttatatt
     9541 caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc caagttcctg
     9601 cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg ttgtagacgc
     9661 tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct gtcgtttttt
     9721 tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt tctatttgtt
     9781 cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct tcgtttgcta
     9841 acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg attccatgtt
     9901 ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca ctagaagaaa
     9961 gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg aatgcagcat
    10021 gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa agctgatgga
    10081 gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc tgtttttctg
    10141 ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct aaacttggag
    10201 ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt tcatggtcat
    10261 gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct tttatcgtat
    10321 ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc atgttttcat
    10381 cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca gaaggttcat
    10441 aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca tccccgacga
    10501 ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat ttttctttac
    10561 tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa accagcatgg
    10621 ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc tttcatttga
    10681 atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag gatttaattt
    10741 atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag aaagtttata
    10801 tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat acccgctttt
    10861 gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac agatacaacg
    10921 aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg aatagttatc
    10981 ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa gcctgtcgga
    11041 atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta ttatcttttg
    11101 gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt cattatcgtt
    11161 tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc ttttcttgaa
    11221 gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt tcaagagagt
    11281 ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt tttcgcttct
    11341 attgacgacg tgacgtctga tatagctgga tttattggaa attattggga aagtggcgca
    11401 caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt atttactggt
    11461 ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc agttttagcg
    11521 attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc taatcaacaa
    11581 ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc agtctactgg
    11641 gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc tgctgggttg
    11701 ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt gatcattgca
    11761 ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc cgtacaactt
    11821 ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat ccatccgatt
    11881 acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg cgttattttt
    11941 ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg taaattcaaa
    12001 caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc attttctgaa
    12061 gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc gtaggaacga
    12121 cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg ttttagctcc
    12181 agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc ttctgtgaat
    12241 ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac aagcagctgc
    12301 tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc ataagcatga
    12361 taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt cttgtaatag
    12421 gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc tagcttgcct
    12481 ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc tgaccattgc
    12541 atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc ttctttttct
    12601 gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc aatatagtcg
    12661 gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata aatatagggc
    12721 gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt tcgatactga
    12781 tcgatagcta caaatcgatt gagcgcacgt ggctgatcat gattattaaa aaatagagca
    12841 ttccagccgt taccctcact catcttttct ccccatgtat gaaatagtcg ctttaattct
    12901 gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac tttcaaatga
    12961 tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa gatacaattt
    13021 tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt cccaaacgtt
    13081 gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata ttcaggtttg
    13141 ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt gataacatcg
    13201 aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa tagttcctct
    13261 cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa gtgcaaataa
    13321 tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga tacccaatcc
    13381 gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc tagtgctttt
    13441 ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat gatttctatc
    13501 cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc aaagatagga
    13561 tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct tggataaata
    13621 gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt ttctttgatt
    13681 ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg atagatttgg
    13741 taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga tttattacct
    13801 accacaactt atatatacaa gttatcattt attattataa ctgaaccgaa agcgtttgta
    13861 aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag agatcagaca
    13921 tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc tataaatggt
    13981 gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg aggagctaat
    14041 tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct ctgattcatg
    14101 gtgttcaatc agctgtcctt cggtattagc tcaaaaaacg gcaaaatatg aaaaactatt
    14161 ttgccgtttg tttctctaat actcaggtca aaccgctgat ttcacaacct ctgtttttat
    14221 tcattcgatt tcaacgcatc aatcatatcg atatgcttta atttgaaatg ggtaacaatc
    14281 atcacaataa ccgtaaatac gatagttaat cctgctgata agacataagc ccaccatgtg
    14341 atcactaatg gaaagatgac attctccata gacgcttgtt gcaagatgaa gtcagtcaat
    14401 atatatccta caccaaatcc tccgatgatt cctaaaagag taaagatgat attttctcga
    14461 acaatgtaca tcgtgacttc tttatcaaaa aatccaagga cttttattgt agataattcg
    14521 cgtacacgct cggaaatatt gatattagtt agattataca agacgataaa agccagcagt
    14581 cctgataata cgacaaagat taagacaacc gagtctaagt tcgccattga ttcttcttgc
    14641 gtttcgattt gtttggacat gaaagacgta tttgtcacag cgtctgtggc aagcagtttc
    14701 tcggaaagca tttcttcttc ttttgttgtc atcttatctg tacgcagcaa aaatgcatta
    14761 tccatcatcg attgatcagt aactgaagca taatattcag gggacatata aataaagcta
    14821 cctagatagt ttttcacgat ttcttttact tcgactttcc attctttttg atcttcatca
    14881 taaagcgaaa tatgatctcc tgtagataag tcaaatagtt ctgctacttt ttgcgtaata
    14941 gcgattcctg ttttaggcaa ggagcgtggg ttctcttcaa gatcagttaa atggataaaa
    15001 cgatccaaat cagctgtttt acttggtacg atcactgtca gactttgcga agcatgtcct
    15061 ttttttctca attctacggt ttgagtatga atagctagga tctcatttac tttttcttgc
    15121 ttctgcagca tatccgctgc tttttgacga ctatcttccg tggatgaatc caatgtaacg
    15181 attgcttgat agtcaatgat cggtccaaat tgtttggctg aaacagaact tagagaatct
    15241 ttcaatccca cgcctgcaac catcagcccg gcacatccag caattccgat aatcgccata
    15301 agcattcttg acttatatcg gaagagattt cggtaactga ttttttgatt aaagctaagg
    15361 cgtgaccaca gcggagtgat gtattcaaga aaaatgcgtt tccctggctt cggtgctttc
    15421 ggttgaagca atgcagcagg ccgttctctt aaatctttga ataaaacgat cattgcagcg
    15481 ccaaatgcgg caatgaagaa tgcggcagct gcttgtaaaa tcggcggcca gtcataaaaa
    15541 ataagcgcac tcccaatatc ataccgctcg ttagacagct caaaaatgat ccttggcaaa
    15601 agattcgttc ctaatatcgt tcctaaaaca atacctatcc ctgaggctag acttgcatag
    15661 attgcatatt tttttgcgat ttcaactttc gtataaccta acgcctttaa tgtcccgatt
    15721 tccctgcggt tttcttctac catacgtgtc attgtcgtaa aggtgatcaa tgcggcaata
    15781 aagaaaaaga aaacaggaaa aacattggca atcgcagcga tacgatcaga cagatcacca
    15841 aattcttgaa aaccgacatt ttctgaacgt tcattgtata ggtatgatgg tttagttaaa
    15901 ttttcaattt tttctgcatt tcgattcaat tcttcctctg cagaggccag ttcacgttct
    15961 ttctcaacta attgcctttc ttgttcagca agttgagctt cttgttgaga taatgtttga
    16021 acagcgattg ttcgttgcgc ttcaggaagc tgctggatag ttgctgactg ttgcgctaac
    16081 tgttttttag catcttctaa ccgtgttctt gcttgcgttg tttgtgcttt gccatccgct
    16141 attttttgct gtgccggttc taattcttcg ttggccgatt ttttcagctc ttctaagcgt
    16201 tcttcgggtc gatcttgtaa atacttttct gccttttctt gattttgctc cattttatct
    16261 ttgtaagctt tgctgtacgt attcaaattc tttacgtttg taaaatcaag atataagaca
    16321 gaataaatat ctgttttaaa aacggattca ggtaaataga caaaataatc gacgctacca
    16381 tttccgacgt tcgcgtatcc tctctccaaa ttgttgatga acaatggaga acggacaaag
    16441 cccgtgatgg tatactcttt ttctttcaat tcatctgaat caatctgata aatatcaccg
    16501 attttatacc cttcatcttc cgctttttca tccaatacaa gctggtttgg tttttccggg
    16561 agttttccat cagttaattg tagcttgttc tgcttgttat ttttgttata agaaaatatt
    16621 tgaatcactt cgtttttgcc agtattggaa taatagatct gttttcctgt ttctactttt
    16681 gcatctggga tcttttctgc ttgagcaata tctttttgtg tcaagccacc agtagaaacg
    16741 atttgcaaat cagaaagtcc tgctgtacca acatagtgat ccatcgtttt ttgcatgaca
    16801 ggtccagcag ttttgacacc tacaaataaa agtgttccta gcatgacaat aaggatgatt
    16861 gcaatgaatc gtcctttaga ttgccgaatt tcgcgaaaac ctgctttaag atatgttctt
    16921 gttttcataa aatcccccta ccatacaatc tcatcaatgg atgaaggatg atcatttaat
    16981 tcaacagacc ttactttagc atcattaatg tgaatgactc tatcagcgat gggcgctaaa
    17041 gcagaattat gtgtaatgat caaaacggta ttgccgtgtt gtctgctagc gttttgcaat
    17101 aatttcaata cttgcttgcc cgtttcaaaa tccagcgcgc ctgtcggttc atcacataaa
    17161 agaagtttgg gatttttagc tagtgcacga gcaattgaaa ccctctgctg ttcgcctcct
    17221 gataattggg acggaaagtt attcaatcga tgagctaggc ccacttgcct caatacctct
    17281 acaggatcaa gcgcatcagg agaaacttcc gttgccaatt ccacattttc ttttgctgtt
    17341 agattaggaa cgagattgta gaattggaaa acgaacccta catctgttcg tcggtaagca
    17401 gtaagctgtt tatctgaaaa ttgcgcaata tctgtgtcat caataatgat ctgaccttca
    17461 tcaggtgtat ccatcccacc taaaatatta agaatcgttg actttcctgc tccacttgga
    17521 ccaagtataa tcactaattc gcctttttca attgaaaaag aaatatcgtc atttgctgta
    17581 atggtcgttt ctcccatttt gtatcgtttg tattcatttt ttacttcgac atacttcact
    17641 ctgattcctc cattttcacg atatttctcg ctatatctat gcatcgaatg cgttttctca
    17701 cacttattta ttttaccact gaatttctct agactaaaga aaggaatcct ttaatttagt
    17761 tgacaaataa acgatttatg atttacttac ataaaaaaca ttgttatttg aaagaaggaa
    17821 catcatgaat gatttaaagg aaaatatgat ttttgattta tcaaaaactg cttttgtcgc
    17881 gatcgacatg caaaaagaga tcgtaaataa cggtacactc tctccacaca ctgcagcatc
    17941 tattctgaca aacaatgatt tgttagtaag aacattgaaa cgtaccgctg ccttgaaagt
    18001 tttagtgaac gtagacatct ccactttccc ttatttgagt caacaaactg atatgggtgg
    18061 aatgactgct tctgtacctc cagaattcac tgatttactt ttgaaggatt ctttgaaaga
    18121 tacagataat atgttgacta tcacaaaata caatccaagt gctttttttg gtacttctct
    18181 tgatcttcaa cttcgccggc gtggaatcga aactatcatt ttaagcggtg tagcgacaac
    18241 aaacggtgta tatgctactg cattagatgc ctttcaacac gggtaccata tcgtgcttgc
    18301 tgaagatgct tgcagtgatc gtgacaaaga atcccatcaa ttgttcataa aaaagatatt
    18361 tccgaaaact gcacgagtac gctcaacgaa acaaataatc gaagctatcc aacaaagcaa
    18421 ataagtactg gcacacgtga ttttcattta cttctttttg atttcatggg aaaataacca
    18481 caggatttag ataggagtat gaacatggtg aaaaaaaaga tcacaatcaa agaagtcgct
    18541 aaactttccg gcgtttcaat cacaactgtc tccttgatat taaatggaaa tacggagaaa
    18601 ttcagtccga aaactgttca gaaagtttta gctgccaaag aagaattagg ctatcagcct
    18661 gactattttg ctagacaaat gattaccaag gaaacgaaaa caattggtgt actcgttcct
    18721 gatatcacga atccattctt cagtacactg atgcgaggaa ttgaggatat cttgtataag
    18781 cagaatttcg tcacgatatt atgtaatgct gattctgatc atcaaaaaga aatcgaatat
    18841 ctagccgagc tcacacgtcg aggtgtggat ggattcatta tcgcgacttc tgctgtctct
    18901 acagatgcaa tcaacgaaaa tttgaaaaaa caaggccggc ccttcattgt tttggatcag
    18961 aaaaaatctg aaggatttag tgatgccgta cgaacagacg attttcgcgg tggttaccta
    19021 gcgggcatgc acttgcttag tctaggacat caaacgatcg cccttgtcta tcctgaaaat
    19081 ccaccggaaa atgtccacgc tcggattgag gggtttaaat ctgctcttga tgtttaccaa
    19141 atccctcacg accaactgat cctcttacct acacagttct caaaacaagg tggctaccag
    19201 atcacagcag aacttcttga ttctgctgca actggtgtgt ttgctttaaa tgatgaatta
    19261 gcttttggat tatatcgcgg attagaggaa gctggaaaaa gcatccctga agactacagc
    19321 attattggct atgataatat agatatgtgc gagtatatca agccgaaact gacaacgatc
    19381 gcacaaccaa tcttcgaatt gggacaaaca tctgccaagt tattgttaga tcggatccaa
    19441 ttcccagaaa aagaatggga agaaaaaaga ttacctgtac gattagaaaa aagattttca
    19501 actgcaccct tgaaatagct ttcaaggtgt gctatatttt tatcgaacta ttaaaacgtt
    19561 ttactaaaac gttttaaaaa ataattttcc catcaaaata aatgagatag ttggaggttt
    19621 gtaaaatgaa cacggttaca gtaattggaa gtattaatat ggacactacc cttcgtttaa
    19681 ccaacatgcc caagccaggc gaaacgatgc atgcgcatga gatttttcac gctggcggag
    19741 gtaaaggtgc caaccaagca gtggcagcca aacgttcagg cgcacggaca tcttttattg
    19801 gaggcgttgg ggctgacagc gaaggacaac agttattaga tctactgaca aaagaaaaca
    19861 tcgatacatc tgggatagct gaaatccaag gcgccacaac cggacaagca atgattatgg
    19921 tagacgcagc aggagaaaat agtatcttga tccatgcagg ggcaaataat gctttccatg
    19981 aacaagaagt cctaaaaaac aagcaactga ttacaaacag tgactttatt attgctcagt
    20041 ttgaaagtag cttagatgcg acgatcctag ctttttctat tgcaaaagac gcaggaaaaa
    20101 caacgatcct gaatcctgca ccagctcgtg aaacgattcc tactgaacta cttgaaaaga
    20161 cagatatcat tataccaaat gaaacagaaa cagaaatcat tacaggaatc agagtgacag
    20221 atcataacag tttggtcgca gcagccgaaa aactccatga attaggtatt ggaacagtaa
    20281 tcattacact aggcagtgcc ggcgctttct atcatacaga aaaagaacac ggtatcgtcc
    20341 ctgcttttaa agtggatgcc gttgacacaa cagccgcagg tgatacattt attggcgcat
    20401 taagcagcac acttcagcca gaccttagta acttgaaaga agcgatcctt tatggaaatc
    20461 tagcctcttc tgttgctgtg cagtcttatg gtgcgcaacc ttctatccct taccgcgaag
    20521 ctttagaaca atcagcagtc taaaattaac aatgagagga agaaacaaac atgaaaaaag
    20581 gaaaagtaat caattcagat atttcacgag taatcgcaca aatggggcac tttgataaat
    20641 taagtatcgg ggatgcaggc atgcctgtcc cctttggtac tgaaaaaata gatttggctg
    20701 tctcaaacgg tcttccaagt tttttatctg ttttagaaaa cgttttagaa gaacttgagg
    20761 tacaatcgat tgaattggcg gaagaaataa agacaatgaa cccggaaata ttggaaaaaa
    20821 tcaaattatt gttaccagat acaccgatta gctttatccc acatgaagaa atgaaaaaag
    20881 agttgaataa ctgtcatgca tttattcgta caggcgaaat gacaccttac gccaatatta
    20941 tattagtgag tggagtggtc ttctaatgga aacgacaatg aatcctgaac agatggtact
    21001 gtccgagcag attcatatgc tgattgaaca gttgggagga aaagacaata ttgattctgt
    21061 ccttccatgt gcgactcggc ttcgtatcaa attgaaaaaa cagccagtag ccaatgcaga
    21121 aaaattaaaa cagcaaatcc cttccattaa gtatttcatt ttactcgata gatatgcaga
    21181 aataatcatg gatcagggat tggctcctgc ttacaaggaa ttgacgcgtc aaatcgatca
    21241 ttcagctccg aaagaaaagc taaatccgat cgaaaaacta tttggtttga tatcggaaat
    21301 ctttacgccg atcattccgc tgtttgccgg ttctggtata ttaaaaggtc tggttgtttt
    21361 agcgactacg attggctggc ttagtgaaac aagcggaact tatcatattt tgtctgctgc
    21421 gtcgaatgcc gtcttttatt ttctgccaat ctttcttgct ttttctgctg ccaaaacgtt
    21481 taaagtgaat ggatatattg cggctgtcat tgctgcagca ctgattgaac cgaatctgac
    21541 ctctttactt tcagacagtg gcaaagcagc tactgatttt ctggggattc ctgtagtact
    21601 gatgcagtat acgtctactg tcatgccggc gattttaggt attttctttt attcttttgt
    21661 tgaaaaattc ctcaaaaaat atattcgcga aaatatgcaa ttagtatttg tacctttact
    21721 ttcattagtt atcacggttc ctttgacgtt aatggtattc gggcctgcgg gtgtttacct
    21781 tggcgaaggt ttagctgccg caattacttg gatgatggat attaacggac cgatcagtgg
    21841 cgcaatcatc gctggtggtt ggaatatttt ggtaatattt ggtatccaat gggctgtaaa
    21901 ccctgtcatg atcagtaata tctctgctta tgggttcgac cgaatcgttc cgttgacagg
    21961 agcagccaat tttggaatgg ctggtgcagc tttaggtgta tttctgcgaa gtaaacgttc
    22021 aaaaacccgt tcgatctccg gttctgcttt tgcttcgatt ttacttgcgg gagtgaccga
    22081 accaactgtt tacggaattg cgatcccatt gaaaaaacca tttgttgctg cttgcattgg
    22141 tgcagctgct ggtggagctg taatgggatt tgcccaagtg aaagcgatcg cttttgtatt
    22201 tggtagtctg accacattgc ctgcattcat ttccggtact tttttctggt acttagcagg
    22261 actggctgtt agtctagtcg tagcaatgat cactactctc gttagcgggt ttgatgaaga
    22321 cctaatgtct tatgaatagt taatgaaaaa gactgaagta tgagatgttt atcacatatt
    22381 tcagtctttt tctttttagg atcattattt ttataaattt ttgtgatact ttccaaactt
    22441 tggtataatt tacatatgca taaccatttt tatccccact tgtttatccc caaacaaagg
    22501 tggtctatgc aataaaatga acgtcaaaga aaaacgaatg aatgctcgtt gcttatttac
    22561 aaattgtttt atttagaata cacagacaga tttatgaaaa gaaaaagctt ttcgacacga
    22621 ttatctgttc caaaaaatgt ctaatgaatg ctgaataaga cagaaaatca atgaaaggaa
    22681 aaggactagt ttattatgaa aaataaaaaa acgatacgtt tgctagctgc tacttggctg
    22741 attgctccgt ctgttttgtc gaccaatacc gtatttgccg tagataatca aatagctgat
    22801 caaacagtca caacagatac gactacatcc agctcagcta ctagtgaaac aacggattct
    22861 tttacagaag acccaactgg aacaactaac tcaacaacag gatcgaccga gccaactgaa
    22921 attgatccgg ctgatccatc agaaaatgaa aagccagccg agacatattc ccttacaatt
    22981 gctccttctt tgattggtaa gatccaactg actgcttcga cagcggattc ccctgaacag
    23041 caacctttgg atattacgga aagtggaacg gtagaaggtt tattagcgga aacgaaggta
    23101 acttacacta ttacgccagc tgaaggtgaa caaattgctt cagtcgctgt taatggtgcc
    23161 aaagaagtaa attatgatgc aaagaccttt acagtaggta cgaacaattt atctattact
    23221 gcagaattta aagctggacc ggatacttca acagagaaac caactgatcc tagtgaagaa
    23281 gtccctagta caccagatcc ggaaacgcca aacaaaccag atgtgggtaa taacaacaat
    23341 aataatggtt caaataattc aaacaataat aacaataatt cgaacagcgg gaattcttcg
    23401 aatcaaacaa acccatctgg atcgaataac aatggaaata ctcagagaga accaaaccat
    23461 ccaaacaatg gttcatcgaa tggcggaacg aacaatgctt catctcgtcc aaatacagca
    23521 aatcaaagcg gtatagagaa tccatcttct gcttcttctg attttgttgt aaaatcacca
    23581 attgatgctg tattgcctac aaatacaacg aatgtccaac aagcaatcgt tcgagaagca
    23641 ttcaagcatc ttggaaaacc atatgtatgg ggagcaaaag gtccaaatac ttttgactgt
    23701 tctggtttga cttattacgt atatatgaaa gcaaccggtc attatatcgg tggctggaca
    23761 ggcgaacagc aatatgcagg tacgcagatt ccagtaagtc aggcccaacc tggcgacctc
    23821 gtcttctggg gaccttcttc cggtgttact caccatgtag gtatctatat tgggaacgga
    23881 caatttatcc atgcgcctca accaggagat aaagttcgag taacttctat ttcagatttc
    23941 acgcctgatt ttgctgtccg agtaaactta gcaggtttac ctgctgcttc aggttcgctg
    24001 acaggcggct catctatttt agatggatta gatggctcgt tccatttcag tcaaaaccaa
    24061 acaactgatc agttcttgaa aaagattgca gatgatgcac aggaaatcgg gcaaaaagaa
    24121 ggaatctacg catcagtcat gatggctcaa gctatccttg aaagtggatc tggaaacagc
    24181 ttattatcaa gcgaaccaaa tcataacctt tttggtatca aaggatcata taaaggaagc
    24241 agcgtgacct tcaatacact tgaacaagat agttccggac aaagctacca aatccgcgca
    24301 caattccgta aatatccttc ttacaaggaa agtttggaag actatgccga cttgatcaaa
    24361 aatggactga ctggcaatcc tgatttctac aaaccaactt ggaagtcgga aacaaaagac
    24421 tacaaagaag caaccaaata tttggaagga cgttatgcta cagatcgcca atattcacaa
    24481 aaattgaacg cgatcatcga agcgtacgac ctgacaaaat atgatgaacc gaaaaaagaa
    24541 gcagcaacaa ctgaagaagc aaacgctgaa atcctcaata tgtctaagcg attcgatgtg
    24601 ccaatcaaat ggacaacaga tggcgtgcca atcacaggat tagaattttc aacgataaaa
    24661 cgcgtcaaca acgcttcttc catcttccgt gtcgcttcaa tctgggattt gtggaacaat
    24721 tttacggcaa gacagatacc agaaacaact actcgtacag ttaaagctgc aactgttcct
    24781 gtactaacct tgttaagttt agacaaatca ttctggcttg atctgccttt aaagaaatag
    24841 aataaattaa aaaggggatg tgacactgtc acactccctt tttaatttat tctatttctt
    24901 tatatttatt ttgtattccg ttacaaactc gcgtacttgg ttaggttcca gagtgacatc
    24961 tccaaaactt tcaagtcgct ccgctcctgg cagttcttga gtctcaaatg tgataccgcc
    25021 atgatttact aatttattcc cgcgcatttc cggtccatct tctccaaaat ttgctgtaaa
    25081 aatcacgatt cctgaagcat ctgtttttac ttggatcgat actttttcat ctgggcttgt
    25141 caattccgca gcatttagta aaccagattg ttttttcaac aaaaatggat gatcgatgcc
    25201 gcccactatc attgattggc gaaaatcact ggcaaagacc tcacctaatt ttttaggacg
    25261 ttggaagtca aatgctgtgc cacttactga aactacttct cctgtaggcg ttgtatcttt
    25321 attgagcgta gcaaataatc ggctattcaa ccaaagtata tgttcgtcga ttgactgtgc
    25381 tggatcaccg gttaaattga aatagacatg atttgtagga ttgaatactg ttttgcggtc
    25441 actgatccct tggaccgtca ctcgccatgt atcatcgttc gtcagcgtat accgtacttc
    25501 tacatctaaa gttccaggaa atccgttttc catatctggg ctagtctgat agaaaatgac
    25561 cgatgcttcg ttttcaccat tgatgactgt ataatgccac tgcttggctt caaagctagg
    25621 tgcaccacca tgtaaacaat gaccagtgtc cgggtctgtt tttacttcga acgtttcatc
    25681 atctaatttg aatgtgccat tcgtaatccg tccagccata cgtccgattg aagcaccgat
    25741 ataagggtct ttttctagat attcttttgc ttcatcaaaa cctaagatca actctcgttt
    25801 ttcattatcg attattgttt gaaaatgaac aacccgtgcc ccaagatcac tgacagacaa
    25861 agatgtccca ttttgattcg tgatcgtaat caagtgatag ttttcgccaa aattttgttc
    25921 attgatttcc atgttccttg caccctcttt tctttcattt gcactgattc cagtatacat
    25981 ggaaaccgtt ttcttaacag cttattccgt gagtaacgag aaaaaattta ctatattcta
    26041 ataaaagacc gtgaaaaaaa ggttgtgaca aaaatattgt cacaacctct ttttccgaac
    26101 aaacggtgtt caaaagcttg catctgcggt aataagccca accaaacgaa aaatatgagg
    26161 agctattttt gcttggctgt tcttattact tgatgcagga cagctttttc acaatctctt
    26221 tttcaatacg attccagtat ataaccatgt cgtcagtaca tagtagatga aatacaatac
    26281 gaagaatatc gtaaacggaa cccaaatatc tttgtatgga ttttgcatga atagaccaaa
    26341 catttgtaat ccgaataaga catggactac acctaagatt cctggtgcta agaataacac
    26401 gcctacttct ctacggatcg attgtttaag caacccgcgt cgtgttccta tcttttcaag
    26461 catttcataa cgagcaatgt cactcttgct tcctgacaag atcttgaaca tcaaacagct
    26521 agcaagcatg gtcaagaaag ctaaacctag gaagaatccc ataaattcaa aaccagagaa
    26581 tagaccatta tacacttcat acatttcata tttctggttg ctgtactcta ctttttgcaa
    26641 agatggattg ttcattttat tttgagcaac aagtgtttcg attggtttca ggtttgcttg
    26701 gaagtctttt acctgaacta gctgtaaagt ggattgtggc aagttcagtt gatcaaattc
    26761 tgattgactg actaccttga tttctttttc ttgctgttcc ggcaattcat agcttcgaag
    26821 ttcctcttgt acagtcagtt cattcatttt tttagcagga actttttctt tcgtgatctt
    26881 cggcggtgtt ccatcttcat gttttatcat gatcaatggt tgagccgcaa attcacttgc
    26941 attatagtaa atcgttcccg catcttcttt ttgagtataa gaagcagaaa gtgttggtga
    27001 aagttctttg attttttgct gatcttctgc ccgaggatta ttcatcacta gatcatatcc
    27061 agaattgttt tctgtcaagt ctgcaatttc atttttaaaa cctaatccta ccgtcaaagc
    27121 gcccagtgcc aaggcaaaca gcatagcgac cattgagagc atttgtgtat attcacgaat
    27181 acgaaagttt aactgtgaca atgtgaaatt gttcaatttt ttcaatgaaa tctgttctga
    27241 tttctttagt aatgataaaa agaaaatgat cacagaatgg aaaatgaaat aagttcctaa
    27301 tacgatcgta actaaggcta cccctatacc aaagacttga taggtcccca cgtgtttcat
    27361 catatagtaa ccgcagccta gaaaagcaat tcccagtact acttcaagga aaaacaagaa
    27421 tttatgctgc ttgattcttg ttggtgtctg atccgcacga atcaatgcta aaatcggttt
    27481 tttgacaatc gaaaaagcat tgacaacagc agctaataaa aacaaaacag caaaaaacaa
    27541 aacagtgatc aacaacccgt gaatactaaa aggtgtaaaa tgcgaaatat tgagatctaa
    27601 ctgatccact aataatcggt ggacgatact tgtcaatcct acaccgacag ctgaacccac
    27661 taaagtagct gcgatcccta ctaagaatgt ttcagaaaaa atcatctgtg cgatttttct
    27721 gccttttgct cccagcatca taaacattgc atagtctttt tgccgcatcg tcatcaaaaa
    27781 ggaatttgca tataaaatat aaacgaatgt gatgatccct agtaaaacag aaccgaatcg
    27841 gaaaatgata actgtagccg caacgatcgt attacttttc aaaaaagctt cgttgcttgc
    27901 cattgattca aacatgtaaa aaatagcaga tgccataact aatcctgaga ataacaccaa
    27961 gtagtctctt aaacggctct tgatcccagt aagtgataat ttccaaagca tagtcaatct
    28021 ccccctactg ttccaagttt ccaagaatag atagtatttc tcgatagaag gaatcacggc
    28081 tttgatctcc acgtttcact tcttgatgga taccgccatc cttgatgaat aaaatacgtt
    28141 ggcaataact tgcagaaaac ggatcatgcg tcacaagaag aatcgataca tgatcttttg
    28201 tattcaattc gtccatcgta tctaaaagat cccgggcact tttggaatcc agcgcacctg
    28261 taggctcatc ccctaataga atcgttggtt gtgtgatcaa tgcacgtgcg gcggcgaccc
    28321 gttgtttttg tccaccggaa atctcagaag gatagctttc caaaatatga gagatggata
    28381 aacgttctgc aattttttgg acacgttgct tgatttcttt tggtctgacg ccttgtaaag
    28441 aaagaggaac agcgatattt tcttgtgctg tcaagctttc taataaattg aaatcttgga
    28501 aaataaagcc aatctcattc gcacgaaaat ctgctagttg atttcctttt aatgtggtca
    28561 catctttttg attgattcgg atatgcccat ctgttggttt atctaatgtg gataaaatat
    28621 tcaaaagtgt cgacttacca gaaccagaag cgcccatgat cccaataaat tcgccttttt
    28681 ctacatcgaa gctgatgccg cttaatgctt gcgtcttttt ctcattgttt tttccgtata
    28741 cttttgttac attctgtact tcgactattt tgttcatttc tttccctacc ctttaattag
    28801 tttttccagt gctgttttcg gcacgtcttt tttttcgact tcttcatacg aagtgacacc
    28861 tttatttttg ttccaggtga tttttaaata tgctccttta cgaagcggtc gagtcaattg
    28921 tcccttgaag tccagttctt tttccttacc ctctttcgta tagccaggca ggatatatct
    28981 atagttgact gtttcttgtc cagagtctga ttttgaaact gtttttgtac catctgtcgt
    29041 aatttgtaca tagtattctt ctccacccat aactacgtaa tctgcgattt tcacccctgc
    29101 aagtaccaca acgatgaaaa caagtagacc gatcagaagt tttttcactt tttccccttc
    29161 tctcttactt tatatactga ttatagaaaa aagcgaacaa ttctgccttc gaatcaacta
    29221 tcattcttct ttcaattttg tcattttctt taaaacaact aaaaaaagag ttcaagacaa
    29281 taatccgtcc taaactcctt cttcctatct tacaaaaagt atgtcactta cggtctttca
    29341 tgtcgcctgt attgataaca ggttgcgttc ctttgtctgt aatgattgag acaagtaaat
    29401 tattgatcaa ttggactttc cgatcatcgg tgaaattgat ttcttgtcca tcttcgattt
    29461 gctctaaggc catttgagtg attgtgacag ctccttcaac aatggtttgt cgagcagaca
    29521 aaatggcttt tgcttgttga cgttggagca tggcactagc gatttctgtt gcataggcta
    29581 aatggttcaa gcgtgtttcg attacttcga cacctgctac tgctaaacgc tcttgcagct
    29641 ctttttgcaa ctcgtcagac actgccgtcg tgtttcctcg aagcgtcaaa tcattctcat
    29701 tgaatgtatc atacgggtat tggctagcga tatgccgaat ggctgtctca ctttggattt
    29761 ctacaaaatc ttgataatac gctacatcga ataatgcctt tgctgtatca acgactttaa
    29821 agactactac tgctgagatt tcgatggggt ttccatccaa atcattgact tttaaaacag
    29881 aactattgaa attccgaact ttcaacgaaa cagtcatttt ttgtgccaaa ggtatcgtaa
    29941 ggaaaaagcc attttctcga atcgtaccta aatattgtcc aaaaaacaaa attacttttg
    30001 cttgattagg actgactaca gttgccgaac ttaacaacaa taacgtgatc aaccaaagaa
    30061 aaatacttaa aaaaagtgcc caaactttcg cttgccacat acctaaatag aagaaaaaca
    30121 atccaactaa agctaatacg accaatccga caatccctgc ataaccattg atataaaatg
    30181 ctcttttttc tctcatgcta cttcctgcgc tcctttatcc ttcaataaat atctaatgtt
    30241 acttttttaa tttttttttc gaatacgcgc cattacacct tctagttcta actctttgtc
    30301 ttcagtgatg taccaaccgc ttgcctgtgt ccctccggct gcctgcggta aataatccaa
    30361 ttttgttcga acccagtgct tttcttctgt aaaatagtct tctaccaaga cctctagatc
    30421 catccctgct gaaagaggca aatcttcccg aacagattta aagataaatc gttccttctt
    30481 cccatcatac ttcaatattc ctgtatcgat aatcggtaaa gaataataat ccagctcttt
    30541 cttcaccgtc aataaatgtt tgaaaacttc atggtacact tgctctaatt ggcgaccttc
    30601 tgaattgttc gggtaattaa ccatcacttt ttctttgtct ggatcataga aggtttggct
    30661 tagttgagtg atataagtat gtaatctttc catttctgtt tttaactttt ctctttgacc
    30721 ttcttctacc ttcatatact ctttcaccct gttttctttt tagatatttt ctttcttatt
    30781 ttactatagt tatgtgcgtt aagcgatgaa aaacagcaaa gattggaagg atctttgctg
    30841 ttttttctta aaatatccag ttgagtacag gatcaatctg tttcattgtc ctcttgatcc
    30901 gtcgtaatat tgatcgatgt gtctttagca gtgatcttta gctttttctg tccttgttct
    30961 aacaaagtat ttgttattgg ctggtagtct ttttcaatat aagattcacc agacgtttgg
    31021 atagtatagc cactgttttc catctcatgc aaagataaat tgccaccgtt taggatgact
    31081 tttccttctt tgctgatcaa actgtcttct agatggaagt tgctgttttc acctgtgacg
    31141 atcaagttat ccacattggc ctcatctaat cgaagtgagc ctaattcatc tttcgcagtt
    31201 attgttcctc ctgtaaattc attgaacgat acccagccat catgtgtctt agtattcact
    31261 tttttgattg atacttcgtt gatatcaatt gcactattat tcccttcgat tgtcaattca
    31321 gataattcat gatctgcagg gacaacaata gtgatttgtg cacttgtgtc ttctaaacta
    31381 atgattgcac ctgttgattt tttgactgca ttcacagtca acacgccatt tttctccttt
    31441 atttctggct tttctgtctt atcataagtc gtttttatct caaaatccaa tccgcgtatg
    31501 accgaaactg tttgattttc tgcatggatg acgattttgt ttgcatcctt gatttttttt
    31561 gtctcagtaa ccatatcgat caaccgagga ccattatccc aagtcagact cttttgagcg
    31621 cctaatgtat aagttgcacc tgctaggagg ccaccagtaa gcatcagacc aataccggta
    31681 atcaaaaaat gtttcaatct caatttatgt gtcctccttc aaacgcccgc tgaatgcaga
    31741 tctctttttg taataaaacg tcgtcccatc catttaacaa agttcatcat gacataaaaa
    31801 agcccttttg ttcctttcag caacagtggc gaaataaata ctgcgcttcc tgtggcgaac
    31861 agaccgacac cgataaagaa taatccactc agaatcgatt gtccaaacac actgattcct
    31921 gagatgatca tgaataggcc aacacctaag atactgatac ataaaacata gatactaaaa
    31981 atgagcgcta gtaaagcaac caatagtccc ccaataacca atataaaccc cagtgctagt
    32041 ggaatgagga caggagaagc aaataaaccg ataatgatta accaaatcag gcgaatttgt
    32101 ctttttggat ttttcactaa ctcgttttct gcttgttctt cttctgaaaa gtaactgatt
    32161 tttaatttta aggcaaactg ctttggtgta ccatattcat caatgatctg atctaatgtt
    32221 tttccagaat ctaaaaaata ttcctcataa tactggatca aatcaaaacg atcgtcttca
    32281 ggaatttctt gaagatgatc cacaacttca ttcaaatatt cattcatttg tgctctcctc
    32341 caataaccgg tctaaagaat ctcgaaaaac aatccattca tcaataattt cttctaaatg
    32401 acgacttccc attggtgtca acgagtaata tcgacggttt ctgccttcaa aaggttcatc
    32461 ataggtctca agatatcctt cttttttcaa tcttctcaaa accggatata gtgtagattc
    32521 actgacagag agactttctt gtacgatttt tgttaaggca tacccataca tatcttcctt
    32581 gcttaatgct gctagcacag taccatcaag aagaacagtt ggaatctgga tgtgcattac
    32641 gtcaccttct attctcaagt gtaatatttt atttcaatct atattataca acatataata
    32701 tagtttcaaa atcagtttgt caaatacaaa aaaactgact aaaagatttg atccttttta
    32761 gtcagcaaaa aatggcttct ctttgatttt tattataaca gtggcataat ccaatttgtc
    32821 cacatccagc tcagtggcat gataacgatc attgcaggta tcatgtcagc tactgggaac
    32881 attttcacgt tgatcattct aaaaccggta gcgaccatca agaatccgcc gcaagcttta
    32941 aaatctaaga tcatgtctgg tgtagtcaat ggataaataa attttgccaa taagaacaaa
    33001 atataaaaaa taatgaattg gggaattgct atcacagaaa cgacataccc taaattacac
    33061 gcaaaaatag ctgctgtgaa aaaatcaagg acagatttag aaatcaaaat cgtactgtct
    33121 cctgtcatcc cagaatctaa acttccatag atccccgtac cacttgcaca aaatagaaca
    33181 atgaccgtca ctaatgtgct gataaattca tcgtgggtca taccaagctt ttcactaggg
    33241 aagattttag aaataggtac ttgcatcagc gcagccccct tatttatccc attacctaaa
    33301 tgaacaatca aaccaatcgc tgttcctatc actaaagcaa aaataacagc cggcatattt
    33361 ttcatcggcg caatggaata gattcccatc cccatcgaac acacaccaaa aaccatcgtc
    33421 agctgtgctt taaaatgttc acttaattta tttcctacta atccgccaat tagtccgcca
    33481 aacaaaacgg aacctacatt gatcaaaact ccagttggca tcaaaaaact cctctctttt
    33541 tctacgaagc gaaaaaaatc agaaaaattt ctgtaattat tgctttcgct gcaagaaaga
    33601 ttgtaacaga cagagaagat tacaccaatt cattttttgt gttaaagtat tctttattgg
    33661 aataagtagg aggaatatga tggatatcca taaattcgaa gtttttcttg atttagccga
    33721 aacaatgaat ttcaccaaaa ctgctgatcg acaattcacc acccaaggaa atatttctaa
    33781 acagattctt tcgttagaaa cagaattaga cgtcaaatta ttcgaacgcg cccatagaaa
    33841 aatcgaattg accgaagctg gaagcttatt gctcccatat gtaaaaaatg tcgtagaaca
    33901 ataccatgct atgcaagaag tgcttgaaac ctatacgaaa gacaacaatc tttcattaaa
    33961 tatcttaacg ataccgacaa tgatcaatta taaaggtttt tccaaaatca ccgagttttt
    34021 gaaacgccat cctgaattta cagtccagtt gaaagaagtg gaaagtgtcg aattaactta
    34081 caatgacgcc aatgaacata gtgacacgat ccactttgct agaagtttcc aggccccacc
    34141 aagttcgatt gagtggttgc ctactgaaac agacgatttt gtagcagttc tacctaaaaa
    34201 tcatccttta gctagtaaaa agaaattgga tctaagcgaa ctgaaaaagg agaacttttt
    34261 gctccttggc cccactacga acttgtatca acctgtacta gatctgtgtc gcgaagctgg
    34321 atttgaacca aaaatctctt ataaaggagc aagaatcgac ttgatcatca atatgattgc
    34381 aaatgacatg gggattgcta ttgttatgga aaaaacagtc aagaatctat taaaagaaaa
    34441 tactgtgatt ctccctattt ctccaacaac agagagttat cttgcattta ctcgtaaagt
    34501 cggtgagcat tctttggctt cagatacatt ttgggagtat ttaaatcaag aagaaaacta
    34561 agccctacat tttctgtcca tctatccacc caaaaaaaga gagcgacgca tgactttcgc
    34621 tctctttctt gcatcatgtc cttgtttcaa tgacacctct aacatttctc gtagttcaat
    34681 aagaaagcag cgatttttat gccaaaagaa ccgtaagaac atttccctaa tcacaaaaaa
    34741 acgactaaaa acccctaaaa accttcaata gaatttttag gggttttatt ctttttccta
    34801 ttgtttactt aaccgtatca ttgctgtcgt tcctactcct tcgatactgc tataatcaat
    34861 acgtccatca tgcagttcta gaattttttt aaccagtgaa agtcctagcc cattcccaga
    34921 aatttgacga cttgtatctc cttgataaaa tttttcaaac attcgatctc tggtctcttc
    34981 attcattcct acacctgaat cggtcacttc cagtacaatc tctgtggctg tctcaaatag
    35041 tttgatatga atctgtccat tgatttgatt gtactttatg gcgttatcca tgatattcag
    35101 ccacacttgg tacaaaaact cttcattgcc tgtgtaattt actgcagcaa gttcgatatc
    35161 caattctagc tgttctttct cccatttagg ctgaaaaaaa acgattactt ctctgatttg
    35221 ttcatctaat cgatactctt tcttttccag ttggatacgc tggttttcca gtttgttcaa
    35281 ttttaagatg ttctctgtta attgtgaaag ctgcgtgata ctttcgataa tccgatataa
    35341 aaatatttgg cgttcttcgt cagagagatt tggggcttgt aacaattgaa catatccttg
    35401 gattgttgct agtggtgttt tgaattcatg ggatacactg gacacaaagt cattcctcag
    35461 tgtctcgata ctattgagtt cttggaccat aacgttgaaa tctttataca gttgctgtac
    35521 ttctgctact ttctgctgtt catccattcg aatcgaaaaa tctcccgttg ctaccagact
    35581 catatttgtc cctagcgcac tgattggatg caggatacgc cgcccaacaa aaatcgatat
    35641 tcctgtaccc acgaccatac tgaaccccgc aaagacgaat aacgggtata acgttggttt
    35701 tccagccgat tcagcaggcg agatagaatg tcgagacaat aagaaaataa aaccgagaaa
    35761 acacagcagt gtcactaaga tcgaaatgaa gacaatacta gcaaaataga tccataattg
    35821 tgaaaatagc ttttttttca tctttaaata accgccttat agcctagtcc ccgtactgtg
    35881 atgatttgaa aatcttccgt cttttcaaaa cgggtcctta aacgtttgat atgtacatcg
    35941 atcgttcgtt cttctgtatt cgtatctaat ccccaaatat catccatcaa ctgttggcga
    36001 gtgaagatct tgttgggata agacagtaat ttatacagaa gcagaaattc tttttgaggc
    36061 agaatcaacg aatgctgatc ttgtaacact gtataagcat ctagctctaa ttttgtttgt
    36121 ccgattatca agctactgct gtaattgatc tttgaacgtc gaagcaatgc ttccacacgt
    36181 aggatcatct cattgacatc gatgggtttt gtcatataat catctgctcc taatttgaac
    36241 ccgcgtttct tatcctcaaa atcggccttt gccgtaatca ttagaacagg tgtgtcaaat
    36301 ctggtgctac gtaatgagtc tagcaagtca tagccatcca ttttcggcat catgatatct
    36361 gtaatgatta attctatacg tgtcgcttct agttttttca gcgcatcctc tccattcaat
    36421 gcggtgatga ctgaaaagcc cgctcgttcc aacacagaac ggtaaagcaa ctgtaattgc
    36481 ggatcatctt ctactactaa aatacgattc attgatctcc ctctgttttt tgaaaaattt
    36541 ctagattttt tcttgtagtt catcttcagt taacatttat ttcgtatact acgatcattg
    36601 ttctgatata cttattagta taccgaatat ttcaatacgg gagaagggtg attatgcttc
    36661 aattaaaaga tataaaaaaa tattataaag tcggcgaaac caccaccaaa gcattggatg
    36721 gtgtgtctgt cgctttcaga caaaaagaat tcgtagcgat tttaggtcct agtggttctg
    36781 gtaaaacgac tatgctgaat gtgattggtg gactagataa ttatgattcc ggtgatatgg
    36841 taatcaacgg aaagtctact aaagacttta aggacagtga ctgggatgcc taccgaaaca
    36901 actctatcgg tttcattttt caaagttaca acttgattgg tcatctaggc attattgaaa
    36961 acgttgaact tgggatgaca ttaagtggtg tttctaaaga cgaaaaacga aaacgggcag
    37021 aagatgcatt gcaccgagtc ggcttgacaa atcatatgca taaaaagcca aatcagttat
    37081 ctggtggaca aatgcagcga gtggccatcg ctcgagcact agccaatgac ccagatatcc
    37141 ttttgtgcga tgaaccaacc ggagcgttag atacagaaac aagtatccag atcatggaat
    37201 tgatccaaga gttatcgaaa gaaaaattag tgatcatggt cacacataat cctgaactgg
    37261 cccatcaata cgcagatcga atcattgaat tttctgatgg taagatctta acagattccc
    37321 atcctcacat cgaacgacca aaagatgatc aatttaactt acgcagaaca aagatgagtt
    37381 tttggacagc gttaaagctt tccttcaaca atatccgaac aaaaaaagga cgtactttct
    37441 tgacctcctt tgcttccagt attgggatca tcggaatcgc gatcgttctg tccctttctt
    37501 ctggattcca aaaacaaatc gataacacgc aagctgaaac gatggcgaaa tttccgatta
    37561 ctatttctaa ggtaacgact aaccagactc gtgacgatgc cggattgggt gcaagcaaag
    37621 cggattatcc agacagcaaa acgattactg caaaagtcag cgaagaagat aaagcacagc
    37681 acacgaataa gattgatcaa acatacgtcg attacgtaac agacatcgat cctaacttaa
    37741 gtaataatat cggatttacc cgtactacag gaatcaatct tttacgtgat gtaaatggca
    37801 aagtacagcc tgtcagcttc tcgaatcaaa atcctgatac ggaatctctt tctttatcta
    37861 gtacgatgtc agcaatgaca ggtgtgggag tgtcaagttt ccctacacag cttgacacta
    37921 gcaaagaaaa ctttttaaaa gacaattatt ctttacttgc cggaagttat ccagcctcag
    37981 caacagatgt cgtgctgatc gtagacggca ataataatac taatatcaac gcattgaaga
    38041 atcttggatt tgatgtgaaa gaagacgaaa aacttgattt tgatgatatc gttggtacaa
    38101 cattcaaatt agtaaacaac aatacttact atacaaaact tccaactggg aattttatcc
    38161 ctaatacaga ttatgatgcg atgtatcaga atgcttctga tgaattgaag atctcgggga
    38221 tcctacgcgt gaaatcttct tctacaatga atctcttatc tccaggaatc gcttatagcg
    38281 atcaattaac gactcaaatc gtcaacgaaa acaaagaatc agaaatcgtc aaagctcaaa
    38341 gagacagtgg tgtaaatgtc ttaactactg aaaaagttga cgaaaatgcc aaacaaacat
    38401 tgcttagtta tttaggtgga gacagcttgc cttcaagtat catgatttat ccgaacaatt
    38461 ttgaagacaa agaaaagatc ttggattatc tagatgatta caataaaggc aaatcagatg
    38521 aagataaaat catttatact gatttagctg gaacgatgac tgaactgact ggcggattga
    38581 tggatgctat cacttatgtc cttattgctt ttgcagggat ctctttagta acaagtatga
    38641 tcatgatcag tatcattact tatacatctg tgattgaacg gacaaaagaa atcggtgtac
    38701 taaaagcttt aggtgcacgt aaaaaagaca tcactcgcgt atttgacgca gaaacgtgta
    38761 ttttaggtat ttcttctggg atattaggtg tgtttattgc atggctagct acgttcccta
    38821 tcaatagcat cttatacaac atgacggatc taaaaaatgt tgctcaacta aatcctgtcc
    38881 atgcgattat tctagtgatc gtctctacta tattgacgat gcttggcggt catcttcctg
    38941 cacgaatggc agcgaaaaaa gacgccgcta tcgctttgcg tgcagaataa ggttgtgaca
    39001 accacattta gatttgagtc ataagagcgc tgaaatagta gaaaacagca agaaacgagg
    39061 aagtaatatg ttccttgttt cgcgctgttt tttttaatta ttttttataa ttctggtata
    39121 cttttctttc ctttgtacca caactcttct ggcgtatacc tgtcttttcc atttatgaat
    39181 actgttccat ctgcttcaat cgttacttca gtcccaatcc acatattacg agaaagtgcg
    39241 agaagatttt ttctttttcc tcttcctttt tttccaaatt gcaaggcatc ggttatctct
    39301 tctgtccaat gagatttcac gtattttact tctccgtttt caaatgtaac tttcaaaata
    39361 ttcgctatat tttccacttt tactgcttta ggaaatgact cctccatttt tttcactttc
    39421 cttcctccaa cactaagtat gcgctttctt ttagtgataa gtatatcata gtttattcta
    39481 ttatcaaaaa aacgcatcca ttccattgta gaatacttta ccgaacaagt tgaattgttt
    39541 tcctcttttt caccttttac tatagatagg taaagacaac taaggaggaa tctctcatga
    39601 cagatcaacc gtatgattta agaaaagtca tcgaagaact aaaaacactt ccaggtcaat
    39661 atcacgaaac agatattgaa atcgatcctg aagcagaatt atctggtgtt taccgttata
    39721 tcggtgctgg cggtacagta aaacgtccga ctcaagaagg accagctatg actttcaaca
    39781 atatcaaagg tttccccggc acacgtgtga acatcggaac aatggctagt cgtaaacgtg
    39841 taggtgaaat tttgcaccat gattatcgaa cacttggtcg tttcttgaaa gacgctgtag
    39901 aaaatccaat caaacctgtg aaagtagaca aagcagacgc tccagcacaa gaagtcgttc
    39961 atctagctac cgatgcagac ttcgatattc gcaacatttt gcctgcacct acaaatactc
    40021 ctgaagatgc tggtccatac atcacaatgg gtgtagtctt aggatcagat ccagaaaaaa
    40081 caatgagcga tgtaacgatc catcgtatgg ttctagaaga taaagatacg atcggtatgt
    40141 acatcatgcc tggcggacgc cacattggtc acttccaaaa acaattcgaa gccttgaata
    40201 aaccaatgcc tatcactatc aatattggtt tagatccagc aattgccatt ggcacgactt
    40261 tcgaaccacc taccactcca cttggctaca atgaactatg gtgtgcagga gctttgcgta
    40321 acgaacctat tcaaatcgtt gatggtatag cagtcgatga agtgggtatt gctcgtgctg
    40381 aattcatcat tgaagcagaa atcatgccaa atgaaacaat gcaagaagat atcaacacga
    40441 actcaggaaa agcaatgcca gaatttcctg gctatactgg ggatgctaat ccagctgtac
    40501 aagtagtgaa agtcaaagca gttactcacc gaaaagacaa tccaatcatg caaacaacga
    40561 ttggtcccag tgaagaacac gtatctatgg ccggtattcc tacagaagca agtattcttg
    40621 ccttggtcga taaagctatt ccaggaaaag tagtaaatgt ctacaatcct cctgcaggtg
    40681 gtggtaaatt gatgaccatc atgcaaatcc gtaaagaaaa cgaagcggat gaaggtattc
    40741 agcgtcaagc agctattctt gctttatcag ccttcaaaga attgaagaca gtcattttag
    40801 tcgatgaaga tgttgatatt ttcgacatga acgatgtagt ttggactttc aacactcgtt
    40861 tccaaggaga taaagatatc atcgtgatcc caggaatgag gaaccatcca ttggacccat
    40921 cagaattacc agaatataat cctggctcta ttcgtttccg tgggataagt gcaaaaacta
    40981 tctttgacgg tacagttcct ttcgatatga aagaacattt tgtacgtgca aaattccaag
    41041 aagtagaaga ttgggaaaaa tatttgaaat aatgaatgat ctgcaactgt tttgacatca
    41101 taaaaaggag tgcggaaaat ggaacaattg gcacaacgga agaaaaaaat cgtgattggt
    41161 gtaagtggcg cttcaggcac catttatgcc atcaacctag tgaagaaact aaaagaatat
    41221 ccgatgatag aaacacacgg aatcatcagt ccatgggcaa aacagaactt aaagctggaa
    41281 tccgacttat cactaactga attcgaaagc ctgtttgatt accattattc aaacaaagat
    41341 ttaggtgctg ctgtcgccag cggatcattt ttgaccgatg gaatgatcat cgtaccggca
    41401 agtatgaaaa cagtcgcagg tatctctgtt ggtttaggcg acaacctgat tgcacgtgct
    41461 gctgatgtaa caatgaagga gcaacgaaag ttagtcattg ttcctcgtga aacgccatta
    41521 aatgccattc acttggaaaa cttgactaaa ttagcacgac taggtgtgca aatcatacca
    41581 ccaattcctg ctttttataa tcagcctcaa acgatccaag atcttgttaa tcaccataca
    41641 atgaaacttt tggatcaatt tgaaattgta aatgagcatt caaaacgttg gcagggtgat
    41701 taatatggaa aaagaaacga tagttgaagt aaccaaacta ggtttcacga tccaagctgc
    41761 tgttaaagta atcggtaaag acttattgat cactttaaca ggcggagata caccacatat
    41821 cggaacagtc actacatttt ctcgtgatac agagatgcaa actgtacgtt tccctagcca
    41881 cgatggtcgg ttccataaag acgatgtctt agccgaaaaa atagctgtga tcatcaaaac
    41941 tgccgttccg ggaagttgta cgatcacttc cggtatccat gttgaccata tctccaatgc
    42001 acaaatcaac gcctcttttc cgatgtcaga agagttaggt tacaagattt tagattggtt
    42061 ggaaaaaata gatttccata tcaaagaacc agtttattat aaagatggtg aaaaaccatt
    42121 atgaaagaag aggttgtgaa atcagcgtta tgacctgagt atgagagaaa aaaatggaaa
    42181 aatagcttct catattttgc cgtttttgag ctaataccga gggacagctg attgaacgcc
    42241 gtttatcaag aggttgtgat aaagccgctt cgtttcgagt agtaagaaaa attttgtaaa
    42301 catgggtttc cacatttttg cataaatttg acttactacc gagaaactgg cttttgaaca
    42361 ccgtttatca agaggttgtg acacgtcttt tgtcacaacc tcttttattt ttgcttcact
    42421 gtaactgcta caatatcacc gatttctttt ccgatgtttt tacgaatatc ttttcgtagt
    42481 ccaaggatgt agcagactga accatctgga tttttcacgc ccatattcac gatactgcct
    42541 tcatacggca cattgtcaaa agttgcttgg actttaactc ttccagcacc gaattctttt
    42601 cttatatcat acggaaaaat aatatatgcc ccgcccttgc cgacttcgga agcatagatt
    42661 tttgattcaa aagaataaat ttccctcatt ttatttctcc tattttttac atctagtata
    42721 gcatagcaaa aaaaagaagg aactgtgaca aaagtcttgt cgcagttcct tttttcgaat
    42781 aaacggtgtt ctcctgccaa ctcctcggtc acaagtcaca atagtcaaca aacatgagaa
    42841 gctgtttttt gaaattgctc cttgtgactc agagttaaac ggcaggctca caacctcttc
    42901 cttaaacggt gttcaaaagc ttgcatctac ggcactaagc ccaatcagat gaaaaatatg
    42961 gagtgctatt tttatctgat tgttcttact gcttgatgca gagcagcttt ttcacaacct
    43021 cctacatggt gttcaaaata ttattcgcct tttgcttctt tcactaattt tgcgaatgct
    43081 tgttgttgga attcagcagc taaggctttc gtacgttgtt cttgctctgc tttgctcatt
    43141 tttggatttt cagtcattgc ttttaccata ccgtcttgaa cataagcttt tgcttcactt
    43201 tccatcgtac tttgctgttt cttcactaat tcagctaact tggctgcttg gtccgtactt
    43261 aattctaccc atgtctctgg gattttgaat gtattcactc ggctgtcata ttcgtgattg
    43321 ttatcagcaa tattgaacca gaatttatac atgtcgtttt tgtattccca ataagtagtt
    43381 ttcgtcactt tttcagcatc gccgtctgtc ttttctactt tatttgtttc gttatccgta
    43441 ccagttgctt ttggtttttc ttgtttttca tttgttcggt aaagatacac tttttctttt
    43501 ccattcccta aagctttata caaaagcata tctgctcctt gggaatcagc actggaaaca
    43561 attttttctg tttttgtttc cgttactttt tccattccaa agtgattgga caaattagcg
    43621 acgatcaaac ttagtgaagc gacgaagatt agtccgaaaa tcaaagataa aaaggtttgc
    43681 catgtttttt tagcgaacac gttcgttaaa gcaaaagcaa gcaagctgac aatcaaaact
    43741 ccaataatca tgcattatcc cccgctttca ttagaacagg ttcttttttc ttcaataaaa
    43801 aggcaataat aaagccaaga atcccaaaga tgatcgctac gataaacgcg gcatggtaac
    43861 ctgacagtgt ggcattgatt gcctgatctt tataagctaa tggcgtatta tccaatacag
    43921 atttgcttgg gatatttgct tttgtcacat tcgtcaacac actgatcaaa atagctgtac
    43981 caatcgagct tgcgatttga cggaatgtat tatttaccgc tgtcccatga ctgatcaagt
    44041 tatttggcaa tgcattcata ccagaagtcg ttaccggcat catgaccatg gagatcccaa
    44101 acatacggat cgcataaagg atcacgatat ataaaactgg agtcgttttt gtcaagaaag
    44161 caaatggtgc agttgccgct gtcaaaatga acatccctgt gatggctaaa cgtttcgcgc
    44221 cgtatttatc gaaaatcgca cccgtcacag gcatcatagc ccccatgatc aatgcacccg
    44281 gcaacaacat caagccagaa tggaatgctg attctccacg aatattttga atatacaatg
    44341 gtacgaccat ttctgcccct accatcgcca tatttgttac accagctaaa acagctgcaa
    44401 tcgtgaatgt agatgatttg aatacacgaa gttccaagaa tggatgttcc aaatgtaatt
    44461 gacgccatga gaacaaggcg atgaaaatca caccaacaac taagaaacca attactttca
    44521 agcttcccca gccgtcatct cctacactag aaaatccgta aagtaaacta ccgaatccaa
    44581 tcgttgataa aatagcggat aaaatatcaa tgctaggatt tgacaatgga ataacacttt
    44641 tcattaggaa gaatgctaaa accaagacaa gaacaacgat tggcagtacc ataccaaaca
    44701 agtcacgcca atggtagtta tcaatgatat aaccagataa tgtagggcct aaagcaggag
    44761 caagtccaat aacgatccct gacatcccca ttgctgatcc tcttttttct ggcgggaaaa
    44821 tagatagcat gatattttgc aataatggca tcgatactcc cacaccgcaa gcttggatca
    44881 aacgaccgat tagtaatgta ctaaaattag gtgctgtaaa gcaagtaatc gttccgatca
    44941 aaaagatcgt catggcagaa agatataaat ttcttgaact gaatttattg atcagccatg
    45001 ctgttattgg gatcatgatc ccgttgacta gcaagaaacc agtcgttaac cattgcacat
    45061 cagacgctga aatatcgaaa gctttcatca atgtaggaaa agctgtcgtc aataaggtct
    45121 gattcaaaac ggtacaaaat gtaccaatta ataagacaac aactaaaagt gtccgattat
    45181 atggttttcc gtaaatatct acgggttggt tgttactcat atttctcctt ctttctgaat
    45241 tttttctgaa actactagaa tactttagtc ctaaattcgc tatttgtcaa cctacttgac
    45301 acataagtta atacggatat aattaaggga agcaagagga caaaaggagt gacaaaaatg
    45361 gttggggctt cccttaaacg tttgttggaa aatgatcaat tggttgttgg catcagtgaa
    45421 ttaagtgata tcgtaggtgt gtctccacgt caattaaggt attgggaaca aaagggattc
    45481 attcgttcga tcgaagctga tgcgaattgt cctagaaaat atcgtttacc tacagtcata
    45541 aaagtagaac tgatcaaaaa atatttagat gacggattta ctttgtcaaa agctgcagaa
    45601 aaggcagaaa tccgccaaaa gaaaatgcac catgtccgta aagttttttc aaaagcaatc
    45661 aaagatatcg aattgatcga cgaacgctat acgattttac aaatcggtaa cttcaaagaa
    45721 gagaatgcta agatgtacat cattcatgac aacgagacag acgaattaac ttacaagctc
    45781 cttcctgtcg atgaaaaagc tgactacgaa cagttgttaa aagaactcta aacgccacta
    45841 atctcctaag taccaagctt tttaataagt agatgctaca taaatacttg aaaaaaggcc
    45901 tgtttgaaca taagaatatc cgaacggtga tctgcgtttc tttcatatag aaaggaattc
    45961 tccatcactg ttcggatatt tttttatttt tttcggttac tttcttctaa tacatctgtt
    46021 agccaatttg catattctgt ttcccgattg atcgcatgcg ctaaaatcag gtagtgtccg
    46081 taattttcat gtttttcttt ttcagtagga aagatcgttt tttttcgctc taatagatga
    46141 agtcgttttt cttcatgaag gtcacgctgc tgctgaataa tttctactaa tgccggatca
    46201 ttcacgtctt tgacgaaata caatttcaga acaaattcgt ctcgattaac cggtagttca
    46261 ttggtgggtg tatgaatcca ctctgccaat tgttccttcc cttcttctgt taattcataa
    46321 actttctttt ccaatttttc acctgtgatc tctacttgat gctgaatgat tccttgctct
    46381 tccatttttg caagttcagg ataaatctga ctatgttttg cttgccaaaa ttctcctatt
    46441 tcatttttga acgattgatt caattcatat cctgtcatct tcttcttact taataaccct
    46501 aacaaaatat atcgaagtgt attttttctt gccatcagtt ttccctcctt gacaaatctt
    46561 taaatccatt ctatcataaa tgagtaatcg aaaacatgta gtttattaca tgttttaatt
    46621 tataaggaga tgcttaaaca tgaaagaatt taaagaattg gaagatttta ttggaactca
    46681 ctttatctat acgtatgaca atggttggga atatgaatgg tatgccaaaa atgaccatac
    46741 tgttgattac cgtatccatg gcggaatggt caaaggccgt tgggtaaaag accaagaagc
    46801 tgatatcgtt aaattgacgg acggcgtatt caaaatcact tggacagagc caacagggac
    46861 agacgtggca ttggacttta tgccaaatga aaagaaactt cacggaatga tctttttccc
    46921 taaatgggtt gaagaacatc cagaaatcac tgttactttc caaaacgaac atatcgcaga
    46981 aatggaagca gctcgtgaaa aatatgaaac ttatccaaaa ttacttgttc ctgaatttgc
    47041 tacgatcact tatatgggtg atgcaggtat agacaatgaa gatgttatta gcgaagaacc
    47101 atacgatgga atgccagatg atatccgtag cggaaaatac ttcgatgaaa attataaacg
    47161 agtaaaataa ggttgtgaaa gagaggttgt gaaatcagcg ttctgacctg agtattagag
    47221 aaagaaacgg aaaaatagtt cctcatgttt tgccgttttt tgagctaata ccgagggtca
    47281 gctgattgaa caccgtttat caagaggttg tgaaatcagc gttatgcttt gagtaataag
    47341 aacgctagtt agaaaatagc ttttcatatt ttctaggcta gtgggcttat taccgagaag
    47401 cagccgtttg aacaccgttt atcaagaggt tgtgaaatca gcgttttgac ctgagtatta
    47461 gagaaacaaa tggaaaaata gttcctcata ttttgccgtt ttttgagcta ataccgaggg
    47521 tcagctgatt gaacaccgtt tatcaaagat tgtaacaact gcatattcac acagttaaat
    47581 tagtgacttc aaaatgaaat aatgctccca gcaaaaaatt aggctgggag cattatttcg
    47641 ttattctgtt catcaaaaat cagcgttttg attccatttc atcacaagtt cctcctcatt
    47701 tactgcttca tctttttgct gtgtttgaat tgtaaaacct tgtgaaagat agaaatgaat
    47761 agctgattga tttttttgat agactgtcaa agtcagtgtt ttattggttt cttttatttt
    47821 tttcattagt tgcgtaccga ttctttgttg ccggtattcc cttttcacaa agattcctgc
    47881 gataaactga tcaatcaccc cagcaaaacc aataatttct tgatctattt ctgctaagta
    47941 aatcactgca tctggtaaag ctttcctaac agattctact tgattctccc aataagttga
    48001 tggaatgaat tcatgagaat ccaagttagc atgcagccaa atcgatagaa cagattccag
    48061 ttctttggca gacaattttg ttgttgtgcg gatcattttt tctctcctta cttattcatt
    48121 gattttaaaa taaaaaccat ttttttgaag ctcttttaca agttttcgcc cctcaaaaaa
    48181 gtatatcttc tcttgtttta cttttctaat accaaaagaa acagcaactt taggatcatc
    48241 agctgaaacg attcgataag ttccaccagg caattgatcc agcttgactt tcccatgatg
    48301 gtcgctttgt actgaaaacg gcacaccatt tctagtaagt gtttttttct tatgtttcaa
    48361 aatgaatgaa ctaccattta ccggtttccc tgacgaatca ctaataataa atgaaaaatc
    48421 aagcactttt ttacttaatc tatactgata gattagtcgt ccgataaaca ctaacagtat
    48481 gactacagct attatcgttc ctgcaagaat aaaccattgc cgcatggttc tttcctgatt
    48541 tccttttcct tcttcttttt ttaccgattc gttataagga atccgatgtc ctgtcactag
    48601 caaacgatgc gtgttgatca tatacggcgt acatgtaagc aaggtaacta agtcttctcc
    48661 ttctgaaatc gttagaacag acgtgtcatc tggcagtacc acttgtatat ttttaacttg
    48721 ataagccaat ttctccccca aaacagtcaa aataaacgtg tctccgtttt ctaaacgatc
    48781 taattcggta aataaccgtc gattaggtaa accgctgtga gcagagatca ctgaatgcgt
    48841 cgaatttcca ccaagcggga aagaggttcc ttgcaagacg cctgctccat tttcaaggat
    48901 cctaccattg agccgatcat aaagtgaaat atttaattgg atcttaggta tattgatact
    48961 tccgatcagc cattcctcta attggctgga tgttgtactt tcatttctgt tcttttcatt
    49021 gaaaggatca gtaccaggat tgaatccttg gtcagttaat cgttggtttt tctcctccat
    49081 tttctttcgc tgcacttcaa tttcagctgc ggtccgtttt tgtacttgtt cgatacgttt
    49141 ttgatcaata aaactattta aagaatccac ataaaatgga tataaagcga tcagtaaccc
    49201 tacgacgata catattaata aaaacaaacg aaacaaaagt cgacgagact gtttttgctt
    49261 actctgcttc acatgtgtcc tcccttgcaa gtttttttgt tctttctttt attatatggg
    49321 ttcaggaaaa aaatagaaaa tgaaagccga tgaggttgtg aaaaagctgt cctgcatcaa
    49381 gtaataagta cagcccaaaa aaatagctac tcatattttt tgttgggttg ggcttattac
    49441 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaaactg cgttatgctt
    49501 tgagtaataa gagcgctagt cagaaaatag cttctcatat tttctaggct agtgagctta
    49561 ttaccgagaa gcagctgatt gaacaccgtt taccgagagg tcatgaaaaa gctactttgt
    49621 ttcgagtatt aagaaaaatt ctgtttcaaa acaaggagat gcaacaaaac ttatgtcgca
    49681 tcctctctcc ttagaacttt cacttatgtt tttcttgtaa aattcataaa tattttcatg
    49741 aattttctaa tttcttgcct acctctcgca taggatatat gatgaattta tctaaagttt
    49801 gcaaaggagt tcgacaattg aaaaaatatg gcccattttt agcaattttt ttcattgctt
    49861 tggcatttct catgcctcat ttattcacac ataaactaat tttgggtgct gatgccccat
    49921 tccattacaa tcggttctat gatgccgcgc aacaaattaa atatggggat ttccattatt
    49981 ttccttccct ttatggattc cagcaaagtg gcaggatcgt gaatgccgtt tacggcccat
    50041 ttttcgctta tttacaagga ttgatcgtgt tgcttgcagg tagctggttt cattatcaga
    50101 ttctttcaaa tttcattctt tatcttattt caggattttc tatgctgatt ttactgagat
    50161 atgtcaatgt tcgaaattgg gtaagtgtac cagtagccgt tttattcatg agtacgtatt
    50221 ctattcaata ctggtctatc aaccaaggat tcactagctg ggggacttgt ttttatcctt
    50281 tatgtatgat ccctattgct gatttcgtcc ttcataaaaa gttccctgtt atcaaagtcg
    50341 ctgtctcagt gggacttatg acacagatcc atttactgtc ttcgatcatg ttagctttga
    50401 tgtatatccc tttttatctt tattactttt ttaaccagac tgaaaaagga aaagcactgc
    50461 ttcaactgat taagtcggtc ttgctttatt ttgttctgac agcaaatatt tgggcaggat
    50521 tagctattgt ttacagtggg aataagattc ttcctccctt tgttaacaga gaaatgagtc
    50581 aaaaggcgat agatctggat ggcagttatt ggctccatta ccctaaggcc tttcctatat
    50641 tgatattatc aggttttttg ctttttattt tctttcaaca aaagaacacc ttattccaaa
    50701 aaatcgtgtt gtacacaagt cttgttttct tttttctttc tactagcttg attccatgga
    50761 cgacattgat cgaaaaaaat gtgcctttta ttagtttgat ccagtttcca tttcgatttt
    50821 tcgtgccatt tacgctattg cttctacttt accttgccct ttcactgaac cagtggtctg
    50881 aacaaaagtg gttccgtctt ttaagtatat ttggcattct tattttttca gcacagacga
    50941 tccagaactt ataccagcat ctggaaaagt gggaaaatga aacatttgtc agtcgacata
    51001 cttatctatt cgatacgccg gaagaagcaa ggaaaacatt cttcagcaaa gatatgtcta
    51061 ccagcttgtt cgttttccaa aaaaccacac ctgactatct gccaatctat aaggagacaa
    51121 aagacaataa atacgaccgg tataacgaag aaatactaga aaacgaaaca acttttataa
    51181 aaacacatcc aggcggtctt ttgacaatca agtggaaaaa cactggggac aaaactgttc
    51241 atatccctgt tatcgtctac gatcggactg tgctacagca aaatggaaaa acattgactg
    51301 attatgaagt aacagatatc gggaccccaa tcgtgaagca gcaaaaagga atcaacgagc
    51361 tcacgcttca ttatcaaacg ccaatctatt tctatttcat tctttctctt actttgattg
    51421 gctggtttac cttgctatgt ttatttattt atcatagata caagctttta cgtgcttagg
    51481 caaaagggat aaatacatcc gatcaaaaaa ctctccaaac ggtataatcg tttggagagt
    51541 tttattttaa tcagtttttt tatgttatat gttatatttt ttattcgttc acatctaatt
    51601 cgatttccac cccgtagtga tcactgatga ttggactatc gatcccatca aaaatccgtt
    51661 gatatttttt aacctgcatt ccttcaggga cgaaaataaa atctatacgc aatttttctg
    51721 tattttgttc ccaaccatct attttctttt ccacagtagc tgcttttcct ttttcattcg
    51781 cgacaacata cgaatcttga agaagaaggc aactttctct tacagtataa taaccttgag
    51841 agtctactgg attattgaaa tcacctagga aaaaaagtgg tacgcgagtt tccaaaaagt
    51901 agttttccaa gtttttccac tcaaaataaa agccactttt tttatctgtc caccaagaaa
    51961 agtggcagct cgagacaaaa atgtcctgat catctatctt tgttttccca atcaagatct
    52021 tgcgtgtctg ataatcttct ttcttttgaa attgggagac ggtaaggacc tgactttcca
    52081 atgttttttt cgtaagcagt gcatttcctt cttcatattt atcataaccg atgtggctca
    52141 tttcccaact ccaaaaatac tcttttccgt gttcctttaa aagctgaact aaacggtagg
    52201 caaaattatc tttacggata ggtacttgat tctttaccgg acagaacttc attagcttcg
    52261 caggttctac ttcttcagat tccagtaatt ggttgatttc ttgtaaagca atcacttcat
    52321 aatcttctaa caggatctta tctgctaatt cttgcaattt tttctctggt tctggctcaa
    52381 gccagctatg cgtgttgagt gtcatgaatt tcattggttt tctccttgtc ttcatttcga
    52441 aacaaaaggg ttgtgacaaa atttttgtca caacctctcc agcaaatagt gttctcctgc
    52501 taactcctca gtaataagct caatggacca ggagctattt tttgtttggc tgtttttatc
    52561 acttggtgca gggcagcttt ttcacgacct ctccactaaa cagtattcaa actgctgctt
    52621 ctcggtaata agcccaacca aacgaaaaat aggaggagct atttttgttt ggctgttctt
    52681 atgacttgat gcaggacagc tttttcacaa cctctcctta cattttaatg gagccgatca
    52741 ctttaccagc tgtcactgta cctaattgct tgatcactac ttcttctact ttatcgtttg
    52801 taaatacgat catgatcgtt ggatcttttt cttcgttttg gatcacatca agatccatct
    52861 cagctaattt cgttcctgtt cctacttctt ggccttcttt gacagcaatc tcaaaagctg
    52921 gctgattcat ttggactgta tcgattccca tatggatcaa aacttcgata ccatctttcg
    52981 tttccagccc aattgcatgt ttgctaggga aaatacttgt cacttttcca gcgatcggcg
    53041 caacaacttc tcttgatgtt ggaataacag caaagccgtc acccatcatt ttttgtgaga
    53101 ataccggatc ttttacttca gataaaggaa tcacttctcc agaagctgcg gaaactagtt
    53161 cttgttctat acctaagtat tgcttactac ctgcttgtcc atttcctgta gaaaccattt
    53221 ctggctctgg aatctctgct cctgattgca gcagatcttc gatatctgat ttcaagacat
    53281 ctgcttttgg tccataaact gcttggactc cattgtcttt gacgatcaat cccatcgccc
    53341 ctgctcgttt ccaggcttct tcagtcccta ctttttctcg atcatttaca cttacgcgta
    53401 accgagtcat acaagcatcg acatctttga tattttcttt tcctcccagc aaatgaatga
    53461 tctggaccac ttgctgatct gctgttcctg ttccgctgtt agatacaacg gtttcttcac
    53521 tgtcattgtc gtaatttccg ttacgtcccg gtgtagcaaa gttgaatttt ttgatcaaga
    53581 aatttgctaa gaaatacgta acaacaccaa aagcgatcac acacaacacg aagttgataa
    53641 gatctccacc tagacccgct ttgatagcta acggtgtccg agtcagcaat tcgatattcc
    53701 caaatgaatg gacccgcaat ggcaagatat ctgccatcgc aaatgcagcc ccttggatca
    53761 ctgaataaat gacatataat ggcacagcag caaacataaa catgaactcc aatggttctg
    53821 ttacacctgt tagaaagaca gccaacgctg ctgaaaaata cattgattta tatcttgctt
    53881 tcttatcagg atcgacatta cgatacatcg caaaagccat tcccatcaag atacctgaag
    53941 aaccaatcat ttgtcctact ttgaaacgag ccggcgtcca gttatgcaac acaaactcat
    54001 attgcgacat atcccctgca cctttaagat tcactaaatc agtggcccaa gccaaccata
    54061 acggatcttg cccaaatact tgtgtccccg cctgtgcgcc tgataaaatc tcatacgatc
    54121 cgccaagctg tgtatagttg ataggaatcg tcagcatatg atgcaatcca aatggcaata
    54181 gtaaacgttc taatgttcca aataaaaacg gtgctagaat cggtgcgctt tcttgtgatt
    54241 ctgcaatcca taaaccaaaa ttattgattc cagcctgtac gtttggccac accacagcaa
    54301 gaatcaatgc cacgattgtt gaccataaga taacaacaaa cggaacaaag cgctttccat
    54361 tgaaaaatga caaagcatcc ggcaattttc tataattata gtacttatta taagccattg
    54421 ccccgacaaa tccagcgatg atccctacga atactcccat attcaaagct ggtgcttcca
    54481 atacacttgt aaaaaatcct ttaaccatga tcttcgtacc gaacagagtg tgggtaaatg
    54541 catcttcatt cgcaagcatg tcacttgtga caccaaagat agccccagtg atccgattga
    54601 ttaaaataaa tgagatccca gctgcaaatg ctccgcctgc tctttctttt gcccaacttc
    54661 ctcctattgc taaagcaaat aaaatatgaa gattaccaat aattgcccaa ccaatatttt
    54721 caattactcc gcccgttgtg acaagcggcg tccaatcagg attgatcaag ggaattgatt
    54781 tccctatact gatcatcaac ccagctgccg gcataactgc taccacaacc attaaagcct
    54841 ttccaaattt ttgccaaaac tcaaaactaa atatcttttt cattccgttt agctccttta
    54901 cgcaatcggt ttcgttaaac gtattatagc gtgcgttttc ttttttgtaa atgctttatt
    54961 ttttattttt ctgtctatac cattctataa aacctataat acgtgtgttc ccttctataa
    55021 ttttctccta aaaatttatt tttttaagct tttttctatg aaatattttc ttttgtctat
    55081 tgacttgtgc aatcggttgc gtttattatg aacgtataaa acagctcatt aggaggagaa
    55141 aaatgaaaca gatcaaacga ctatttgcta ttgatccatg gaaaatcagc acgacacaat
    55201 tagataaaaa aaatcttcga ctgcaagaat ctttaacaag tattggaaac ggttatatgg
    55261 ggatgcgcgg gaactttgaa gaaacttact caggggacca ccatcaaggt acttatttgg
    55321 cgggtgtctg gtatcccgat aagacacgtg tcggctggtg gaaaaatggt tatccagaat
    55381 actttggaaa agtgatcaac gccttgaact ttatcgcgat agatatttcg atcaacgatc
    55441 agctgatcga cttggcttct ttggaaccag aagactttta ctgggaatta gatatgaaaa
    55501 atggtgtatt atctcgtaca ttcacaatca ctacttctac taataaagta cgcatttcct
    55561 tcgaacgttt tctgagcatt gtcaaaaaag aatcagctta tatctattta actatagaaa
    55621 tgcttgaagg tagcggaaca gtcaaactcg tatcaaaatt ggatggaaat gttcaaaacg
    55681 aagacagcaa ctatgatgaa catttttgga atgagattga tcgtggaact atagaagaaa
    55741 tcggctattt gacgactaaa actattccaa acaactttgg gattgaacaa tttactgtta
    55801 ctgcagcaat gcgccatttc gttaatggaa aatcagtcat ccctgactat cacgaagata
    55861 ctttagcatt acatggttgc ttgtctgcag aattatctca aggtgagaca tggtcagtgg
    55921 aaaaagaaat tatcgttctt actagtagag atattccaga agcgcagcaa gcagaaaaag
    55981 ctgtctcaga attaacgaac cagacaaaca attatatcca agcaaaagaa gagcaaacag
    56041 cggcttgggc agaacgatgg aagcttgccg atgtggtcat cgaaggagac gaagaagcac
    56101 aacaaggtat tcgattcaat ttgttccaac tattctctac ttactacggc gaagatgaac
    56161 gattgaatat cggccccaaa ggattcacag gagaaaaata cggcggagcc acttactggg
    56221 atacagaagc gtatgctgtc cctctttatc ttgctttagc caaacctgaa gtaactaaga
    56281 atctgctgaa atatcggcat aatcaactgc cgcaagcaat ccacaacgct cagcaacaag
    56341 gattaaaagg cgcgctttat cctatggtca ccttcactgg tgtcgaatgc cataacgagt
    56401 gggaaatcac attcgaggag attcaccgaa atggcgcgat tgcctatgca atctacaatt
    56461 atacgaatta caccggcgat acggaatatt tgaaaaatga aggactggaa gttttagcag
    56521 aaatcgcacg cttctgggca gaccgtgtcc atttttcaaa acgagcaaat aaatacatga
    56581 tccacggtgt gaccggccca aacgaatatg agaacaatat caataacaat tggtatacaa
    56641 atacgatcgc agcttgggtc ttgcgttaca cgcgagaaaa ttatttaact tatcaaaaag
    56701 aagctgctat cactatcagc gaagaagaac tagccaaatg gaaagatatc gcagagaaca
    56761 tgtacttccc agttgacgaa gaattaggaa tctttgtcca gcatgacaca ttcttagata
    56821 aagatctcat gcctgtatcc gatattccca caagcgatct tcctctgaat cagcattggt
    56881 cttgggacaa aatccttaga tcttgcttca tcaagcaggc agatgtacta caggggattt
    56941 atttcttcaa tgatactttt acaaaagaag aaaagcaaaa aaactttgat ttctatgaac
    57001 caatgactgt ccatgagtct tccctttcgc caagtatcca ttcgatttta gcagcagaat
    57061 taggaatgga aagcaaagct gtagaaatgt accagcgaac agctcgcttg gatttggata
    57121 attacaataa cgacacagaa gacggcttgc acatcacttc catgactggt agctggttgt
    57181 cgattgtcca aggatttgct caaatgaaaa ctgcccatga tcagttgaga ttcgcaccat
    57241 ttctgccaaa aacttggaaa gcttattctt tccatatcaa ttatcgcgga cgtttattgt
    57301 tcatctcagt cacagatgaa caagttactt tcgagttatt agaaggttct cctctagaaa
    57361 tttacgtcta tgaacagtct tattctttag cggatcgtct agaaataacg ctaagaaagg
    57421 aggaagcaaa tgtttaaagg ggtattgttt gatttagacg gtgtcattac cgatactgct
    57481 gaattccatt atcgtgcttg gcgaaagctt ggtcaggaaa tcggcattac gatcgaccac
    57541 acattcaacg aacagctaaa aggcgtcagc cgagaagact cactctcttt gcttttagct
    57601 tatggcggaa aagaacactc tttttcaaag gaagaatttg cagaattagc gaaaagaaaa
    57661 aacgattatt atttagagat gatccagacg atcgaaccaa aagatgtctt ccccggggtc
    57721 gtgcctttac tggattcttt aaaagaagaa aaaatcaaga ttgctcttgc ttctgcaagt
    57781 aaaaacgggc cattcctact tgaaaaaatg ggtcttacac cttactttga tgctattgcc
    57841 gacccggcag aagcaacaaa cggaaaacct gcgccagata tcttcctcct tgcagcaaaa
    57901 gctgtcggac taaaagcaga agaatgtatc gggatcgaag acgcgcaagc cggtatccaa
    57961 gccatcctct caagtggcgc tcaaccaatt ggcgtgggac gtgctgaaga ccttggagag
    58021 gagatcccgc ttgtccctga tacgactttt cttacgatcg attacttgaa agaaaagtgg
    58081 catgaccatg gataatccag agatctccgt cgtaccagtt ccagagactg aaataaagaa
    58141 aatcacattg aagaacgact acttagaagt cgttcttctc aattacggtg cccgcctgca
    58201 ccaaatcttc gcaccagaca aagaaggaaa atcagaaaat gttttattgt cttacgatcg
    58261 tttcgaagat gttctaacag acaaaagctt ttttggagca actgtcggac ctgtagccgg
    58321 aaggatccgg gatgcttctt ggggtgagca tagattggag aaaaactgcg gatcacacca
    58381 tatccacggt ggcacgaaag gctggtcttt ccaattttgg gaagtagaag ttttcaaaac
    58441 tccatcatca ataggtgtgg tcttttactt aaaagatgaa ttttctacct atccaggacc
    58501 aatcactgct acgatcactt accgtttaac aaaaaacgaa ttagagatga caacctctgc
    58561 tagtagcctt gttgaaacca tctgcaatcc gaccaaccat gcctacttca acctttcagg
    58621 aaatggaaca agagacatct atgaacatca gttaacggtc tttttagatg gtgtgcttga
    58681 attagatcaa gaaaaactgc ctactggaaa ttggacaaaa aaagaaaaac tgccaatcga
    58741 ctttagaaaa tccccaacac tgcaagagat ccttgcatgt tatccagatg gactagatga
    58801 tgtatttctt ttgcatcatc ctaggctttc caaaacttcc ttacaattat tcgagaagca
    58861 ttctggtcgc caaatgacta tagcaacttc taataaaagt atggtattat tttctacgac
    58921 tggattcgaa gctgatttct ctgtcaatgg aaaacagatg cattcaaact acggattagc
    58981 aatcgaaccg caagaaatcc ctgatatcgt tcatttcccc aaatttggat cgatcaacct
    59041 ccatcctggt caagaacgga tcagtcagac gatttatcgg ttctctgcac aataacaaga
    59101 gaaaggtgtt tttatcatgg ctattaccgt aaaagatgtg gcgaaaaaag ccggtgttgc
    59161 cacctcgacc gtctctcgtg tcatcaacga tcatcctagt atttccgaga caacaaagaa
    59221 gaaagtccga aaaattatgg atgaactagg ttatgttcct aacatgactg cccgcaatct
    59281 aggaaaacgc atttccagtg ccatcggcgt aatcttaccc cctcttgatt caaaagagcg
    59341 gatcggtaac ccattttatc ttgagatcat ggaagcaatc aacgaagaag cgcggaatta
    59401 tgatatgact acagcaatcg ctactgctaa aaattttgaa ttgctgttag agaacgtcca
    59461 gcgtatgcat ttacaaaaac aagtagatgg ttttattcta gtgtattcag atagtcaaga
    59521 tactgtcatt gattatttat accaaaacca catccctttc actttgatcg gacagcctta
    59581 tcatcatgag aacgaaatcg tttatgtaga taatgataac cagcttctag gtaaacaagc
    59641 aacagaattt ttgatccaga atggacataa gaatatcctg tttgtgacaa acacgactca
    59701 tgaaagtttg tatttcgaac gatatttcgg ttatcaaaaa gcaatgatga tggctggtct
    59761 tcctgcgtta tccagtgtag tactagagca tccggaggat tatctgtcct ttgagaaaac
    59821 gttgaaagaa aacgaagcca cagcattggt ggtcattgac gatatcttcg cccttcgcac
    59881 gatgcagttg gcgcaaatgt atggctatgt cattccagag acactctccg tcattagctt
    59941 caacaactcg atcttctcaa cattaacaca cccttacctt acaagtatcg atatcgatat
    60001 ctccgaatta ggacgtgtcg ctatgcaaaa gctgcgcgaa ttgatccatg aagaattgag
    60061 cagtggcgta cgtttggtca tccctcatcg cttgatcaaa agagagacag tcgttccttt
    60121 agaaaaccca tactaaatag ctgactcact aaatacgctg accatttatc ctatggtcag
    60181 ctttttatgt aaaagaagat actacttcta acacattgtt tatctgtaaa aaagagcgtg
    60241 ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcggaagcag
    60301 aagcaactcc ttcggaaata agctgaaatt cacaaaaatt taaaaagcaa ttttcttgag
    60361 tttcttctta tttcttggag ttaaacgctt ctgtcccagc ctcttttttc cttccttcaa
    60421 ttttttacac gtcgacgatt caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga
    60481 atcgagaaaa aggcgaatgg caagtaatgc aacgcgctaa ctttcatcgt tcccataatg
    60541 aatgtgccgc tgactcccca cggtacgaga gaattgacgg tcgcacctga atccgctagt
    60601 gttcgtgtca aataggtttt tgataagcca acgtcatcaa aagaacgctt gaatgtttct
    60661 cctggaagaa tgatggacag atactgttct ccaacgatca gattgattcc gatacagctc
    60721 aaggcagtaa acccgactaa acgagaaggt cggtccatct tttcttttaa ttcttgaacg
    60781 atggtatcaa cgatcagata tttgatcagt aacccaccta atgccaatgc aagtaaaatc
    60841 aaactagcag aacctaacat actcatgatc ccgcctcgag aaagcagttc atctagcttt
    60901 ggatcaggtg tatttgcgac ataaccattc atcaagatgt ctgctgcttt tccgaccgtc
    60961 aaacttttct catggataaa agaaagaacg agagcagctg tcgatcccgc tagtaatgta
    61021 gggatagccg gaattttttt gattgccccc ccaaataaaa taactagtgg aatcaatgtc
    61081 caaggagaga tccaaaagct cgctttcatg ttttggatca tttcattgat actagctaga
    61141 tcggctgaat gactgttatg tcctaatatc gtataaaaaa tcaacgaaag gacgaatgct
    61201 gggaaatccg tataacgcat accagcgata tgttcgaata gattaactcc tccaataccc
    61261 gctgccaagt tggtcgtatc tgataaaggc gagcagttat tccctaaaaa ggcaccggaa
    61321 acgactgcgc ctgtagtcat tgcatcattg aatccaagta aatgaccgat ccccaaaaaa
    61381 gcaattccaa tcgtcgaaat cgttgtaaat gaactgccga cagtcactcc gacaattgca
    61441 caaacaagaa atgctgtcgg taaaaagaaa cgcacagaaa caagtttaaa tccataaacc
    61501 ataatcgtag ggatggttcc agataaaatc catgaagcaa ctaagacacc aatcaaaaga
    61561 aaaatcagga ttggaatgat ccctggagtg attcctttga tgatcccgtc atggatctca
    61621 tcccatgtga actttttaaa tctgccataa aacaacaaac acatgaaagc tgcaaggatc
    61681 gggatatgtg gcgagagctg gaatccgatg atcagtactc ctaggagtgc caacaacacg
    61741 atcaatacag cgatactttc ttttaaagaa atatttttat tcatcgtcga tctcctattt
    61801 caaatgaaaa ggccctgcca tgaaaaaact attcacttgg caggacctcc tactaaatca
    61861 gcgacaaaac gtcggctgaa agacttattt ttttattttt cttctaaact tttttctacc
    61921 tcaatccccc aagaatcttt cttgaagtga ttgatgatca atggaataac gaacatgacc
    61981 actgtgatag ctgctactaa taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat
    62041 acgacaacga tgatagagaa gatcagcatg cagctgacta cccaagctaa tccgtttccg
    62101 tttttaccaa ttcggtaagg acgttcgaat tctggatgtt tcttacgcat tttaagcaat
    62161 gaaacagcaa tcaaaatgta cacgatacaa taaataacag ttgtagcatt tgtaagtgtc
    62221 aagaatacag cacttacgtc atccattaca ccataaagca aagcaaataa tgaaataacg
    62281 attgattgtg ttagaacgac atttctagat acgccgtatt tgttttcttt atggaatccg
    62341 aattttggtg gaaggaatcc ttcacgagct acttggatga ttgtttttga tggtccagta
    62401 acccaagctg ataattgtaa aagcacacca ataaatacca tgaagctgaa gatatttccg
    62461 atcacttctg gtaagcctag gatttgacag tcaatcaaga taggctgcgt gatatttgct
    62521 aattccattt ttccatctgg tacgacattt gctacaagca tcgcattgat gacattcaat
    62581 aaaactaatc cgatcaaagc gataaatacg ccttttgtat aattttttgt tgcgtctttc
    62641 aaacgaggca tgtaaacaga actcatttcg atcccaacaa aaataaatgt gataccagct
    62701 aagtatttca atgaatcaat atgttccaag tcagggaaag cttttgacca tgaaaagtca
    62761 cctagatagc cgtttgagac tagtccgact ttgaaggctg ccaaaacacc taaaacgaac
    62821 atgatgacta ctgggatata tacccctagc catacaccga tgttcccgcc gacttttgcc
    62881 atatcgaatt tcaagttcaa gatcgtgatg atccagtaga taactaaaat acaacctaaa
    62941 acgaaccagt gattattgcc aagttctaca ttaccaaatg tgtttcctaa caatggtcct
    63001 aaagtagaag cgaccattac catacctggg aacatttgca cccataatag ccatgcaacg
    63061 acaaatcccc atttgctgcc cagtgctgtt tttacccata actgaggacc gccttcttga
    63121 ggaagcatcg tggaaagctc accggaaacc attgatatcg gaccggcaaa aaacaaaact
    63181 gcaaaaattg aataaaaaat caatgtccaa ccgactgcgg ctaacgtagg aatactccga
    63241 accgttgcac acagtgccat tgtcagaccg ataaaggtcc caagacccat tttacttact
    63301 tcagatttac tcaccgttga cactctcctt gattttttgg ataaaataac ggtgtctttg
    63361 aagtagtttc agcaataaac gaaacaattc agcgaatatt tgaggggatt ctttgagttt
    63421 attgcttgaa gggtttcttc aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc
    63481 tccccttgct cttcagatcc aaacatgcgt cagaaacagt tagtattcaa gctgttttct
    63541 aacatgatta ccttgcttat tttgcttcgc ttgccaataa ttggtctgcg tagatttttt
    63601 ccaatttttc ttgaagagct gctttgattt tttcagcata ttcttcaaag tgttcttctt
    63661 tgttcatgta tggtgtcatt acgctggcac gcaatacagt tacttttccg gcacggttcc
    63721 attcttcatc tgagaatcct agttggttca caaattgtaa tgggctgttg ccatagtctg
    63781 ggatagcaaa gtcagtatgt gaagttaaga actcattacc gtaaatgctt cctttcacgt
    63841 atgaagaata atcatatacg tcatggttca atttattcat tgcaactaag tcatcgttgc
    63901 ctttttcttt gaatacatag tctaccatat tgaaatctgg ataagttaat ggatgaactt
    63961 cgatttcttt gtctcctact ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg
    64021 caccttcgat tgatgcaccc attaatttac catatcctgt gacattcaaa ggtaagacat
    64081 ggtgtgctgc ccaaacgctg gctgctgttg cccctgcttt tgaaccttcc aagatataag
    64141 ccccaagtaa tgctgggata tctgcgcctt tttcaaatac atatgtcgca aagtaagaga
    64201 taacgtctct catgcggata tctttgatga cgataccacc ggcagaatat ggaacatagc
    64261 ccattttatg tgggtcaatc gttacagatt ctgcttcttc gattgcttta tatgcactgt
    64321 gtacttcttc taagatataa tctttgtttt ctgtaaagac attgtatttg taatgaacat
    64381 cttttaaatc ttcaaatgga atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt
    64441 atccgccata agcagcatct acgtgtaagt agaagtaaat accgtctttt tctaatacgc
    64501 gacgcaatgc aacgatttta tcgataccgt cgattgctcc ttcttctgtt gatcctacaa
    64561 caccgactac acctaggatt ggtgtttttt cagctgctag accacgaacg attttttcaa
    64621 gttcgttgat gtccatgcga tagttgtggt caactggcac tggaatcact tggtctaaac
    64681 cgataccgat gatgtcggca gcttttagcc atgaatagtg tttagtttgt ggaaccaacc
    64741 attttcctaa tttttctaaa tgttttccgc tacgtgctga gtgagctttg atttcgtcga
    64801 ttttttctgg gacgctgtct aacagattca tgatttcttc tgttgacaag ttcattaatt
    64861 cccaatcgct ttttccagca actagttcag gtgtcacttc tttcattgca agcggcaatg
    64921 atttgatgtt gcgggcatac caaagtcctt ctaagtttgc taaagaacca tcagcaacga
    64981 tgtggcccca gccatcttta tagctcatta attttgcaaa ttccattcct acttcttctt
    65041 ccatttggct tgttgcagga gaagattcat aggcaacgtt attaccattc catagcatag
    65101 caaaattgta cgctagtaaa gatggcatca atgtttcaga gttcatatga ccccagtaac
    65161 gtccggcatt atgccaagga acggaatggg tacgcatccg cgctgaaatt tctgatagaa
    65221 catcctttgt tttattcaca gtgtgttcaa aactagcact gcttttttct tctggcgtaa
    65281 taataggcat gtcttgaggc atgtagtttt gacgccagcc taaatgttca tccaccaatt
    65341 cattcaacaa agctttatag atttggccgt tctcagcttt gtccccaatg aatagggcat
    65401 ttaggtttag atctttcgac aatgattcac tcatagtgaa accctcctaa aaaaatattt
    65461 aaattttgtc gagccctaca ttacacctgt tcttctattc tgtaaactaa gttttggcct
    65521 gatttttaat catattttaa tatgtatcaa ttatccgaat tgttcacact tttgaaataa
    65581 ttaaaaaaaa cgagggaatt tacaaatgta aactccctcg caaagattgt attaacgaca
    65641 accagcgttc tattttagga tgggtttcaa aattggaatg ttgtaatagt atttcccttt
    65701 tttaaggtaa aaacttacta aattctaatc aattactttg gccaaaaagt aatttttctt
    65761 accttttcga ataaccacaa acttaccatc gaattcatcg gatggattta tgacaaaatt
    65821 taaatcggta acgcggtcac cgttaatact tatagctccg tttgaaacat cttcacgtgc
    65881 ttgacgttta gatggttcaa tttttgtgga aacaagcagt tccacgatat tttcaggtgt
    65941 gcttgaaatt tcaacgttcg gcattttacc gaatccttgc gcgatttctt ccgcatttag
    66001 ttctttaata tttccagaga acaatgcttg cgttattttt tgcgcttctt tcaagtcttc
    66061 ttcactatgc acaaatcttg taacttcttc tgccaaacgg cgttgtgctt cacgtttctc
    66121 aggttctgtc tctacttttt tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa
    66181 gaatttcaag tatttcacta catcgcgatc atcttgattc aaccagaatt ggtagaactc
    66241 aaatggtgaa gttttcttcg gatcaagcca aacggcaccg cctgcagttt ttccgaattt
    66301 cgttccgtca gccttcagca tcaatgggat cgttaaaccg aacgcttctg cttctggtcc
    66361 ttctaatttt cggattaggt ctaatccagc tgtgatattt ccccattggt ctgctccacc
    66421 gatttgcaat tgcacatcgt atgttttatg cagatgcaaa aagtcgatag attgcaggat
    66481 ttggtaagta aactctgtaa atgagattcc tacttccaaa cggctggcaa cgatatcttt
    66541 tgctaacatc gtgttgatgt tgaagttttt accgtagtct ctcaaaaatt caagtaaaga
    66601 gatttttgac aaccagtcgt agttgttcac aaatgtgata tttgcatctt taccaaacaa
    66661 ttttctcatt tgattggaaa gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa
    66721 cacacgttct gttttgcggc cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag
    66781 aatatatgga tgatggcctg ccaattggaa tcttttcatc atcataaatg gaatcaaatg
    66841 tccgatatgc atcgaatcac cagttggatc aacgccgcaa taaagtgaaa tgctcttttc
    66901 ttcaacgagc tcgcgaagtc tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt
    66961 taattcatca ataatattca ttttactgac ctccgtgtag tttttttatg aaagcaggaa
    67021 cctgctagca tatgtaatta attatttctt caggatagga taggaaataa aaaaagtccc
    67081 ttggcatata agccaaggga cgaaatctcg cggtaccacc caatttgtat gtctagatat
    67141 ccaacatacc tctcgaacat ttaacgctgt cgcgtggcaa tcactgccaa agactcctga
    67201 aatgtaattc gctttaaagc atgtactggt ttgcagcgac caccagcttt ctgaaacatt
    67261 ggctttaaca actacttcgt ttcgatcttc gtcttgttat tattaatatg tgtctatcat
    67321 agagcgcaac tttttctttg tcaacagaaa aaggatattt tttttaaatt cctcattttt
    67381 tcttttttga atccgctcta attccgaaca ttttattttc aattattgtt aaaagaaata
    67441 actttttctg tattttttct gcaatttatc gagaagtcac tttactttgc ctcttattct
    67501 ttcttcgtta caataaggaa tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat
    67561 atggaaaaaa agcaaaaaat tattttagga gccacttctg ctttaactat cgttatttta
    67621 attctaagtt tgttttttgt catccgatca tctacaaaaa aagaaccaat cattcctgaa
    67681 aaaaaaataa cagaaaaagc aaaagaaccg acagatgata agcaacaact agttactcaa
    67741 gcaaaaaatc tggctcaaag ctacgattat gaccaagcga ttgcgttatt ggaaaaagat
    67801 agtaagaacg gatctactga aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa
    67861 caactgatcg actggtctga tcctactcag atctctcatg tctttttcca cagcttgatc
    67921 gtggacccgc aaaaagcctt ccactctcaa caggcacaag gatacaaaga ttatatggtg
    67981 actgttgaag aattcaaccg ttcgatcgaa caactttatc aaaatgattt tgttttagtt
    68041 aatttgaatg atttgatcca aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg
    68101 ctacctgaag gaaaaaaacc attggttctt tcacaagatg atgtcagcta ttatgaatat
    68161 atggataatt ccggttttcc tagccgattg gtcgtatcaa ataacaatga aatcaaaaat
    68221 gtctataaag agaaagataa agaagaaatc ggtgactttg acatggttcc tctgatcgat
    68281 gcctttgtca agaaacatcc tgatttttct tatcgaggcg caaaaggtac cttggccctg
    68341 acaggttaca acggagtttt aggttatcga acatcaaaat ctcaatatgg agacaatgaa
    68401 aaaacaaaaa aagagattaa agaagctaaa aaagtggcgg aacagttgaa gaaagacggc
    68461 tggagctttg cttcccatac atggggtcac ttgaatatga ctcaagcttc actagaagat
    68521 gtaaaaaaag acaatgaatt gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat
    68581 attctgattt atccatttgg tgcagacatc agcgactggc agccttattc agcagaaaat
    68641 caaaaatttg cttacttgaa gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct
    68701 acaccagctt ggggacaatt aggtacggat ttctatcgaa atgctcggat caatatcgat
    68761 ggcattcgtt ttcaatctga tttagacgga aaaaatccta ttcttgatcc attcatcaat
    68821 gtccatgatg tctatgacaa agaagcacgc gaatctgctt gattttacgt catttatgaa
    68881 ccaaaaaagc cgaactgaca ttttatcgtc agtttcggct tttttatgct ttatttcttc
    68941 ttcggtgcga ttcccttgat caggacttct gtccattctg ctgtatatgg aagcagtttt
    69001 gcgtatatct ccggcttaga aattccttct accattgctt gaaaatagag gatcaagaac
    69061 tcatccgaat atttcaaatc cacttttcct tctttccgcc ctcgatggaa aagatcaagc
    69121 atgatactga aactttcttg cgtatacgca tccatcgcgt cagaaaattc ttttttttct
    69181 ttttttgtat agtatgtcat caaatcgata taaaattgtt tgttcactcg attgagataa
    69241 cggattttgt tttcactcat ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc
    69301 aacgctttcg cttcttgccc taaatcattc ataaatgcaa aaaatacttc ataaatcagc
    69361 ccttccttgc tgccaaaata tttaaaaatc gtcgctttac ttacctgagc gttctcagct
    69421 accttatcca ttgtcactcc agcggttcct atgtctgtgt tcatcaagtc aaaagctgct
    69481 tgtaaaatct ctttcttttt ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc
    69541 tcccgtaatt ttaaactttt ataatatgta tagttcaaat ttattctgag gtttattata
    69601 actatctatt gagaaagtat caagctagca attccttgtt tatgaattca ttgacagaaa
    69661 tttataaaaa gaatattatg tactaaaata ataaaaaaag ttttataaaa aggtataaaa
    69721 cctttttatt aaactattta atcttgttta gtatatattt ttagaaaaag gagtggggtt
    69781 tttatggcag aaatcgtcaa agtcacacat ttgaaaaaga gctttggtcg tttcgaagct
    69841 ttaaaagatg tctctttcac cgtacattca ggtgaagtcg ttggatttat cggaccaaat
    69901 ggttctggaa aatccacgac gattcggaca cttttaggga ttataaaaaa agacggcgga
    69961 acggcaacga tttttgggca agatgtatgg gaagatagtt tatctatcca tgaacgtctt
    70021 tcttatgttc caggcgatat ctctttgtgg ggaaatttga ctggaggagc gatcatcgat
    70081 ctcttcatga agcttcatgg aaaagggagt gctcaaaaaa aagatgaact gattcaacgt
    70141 ttctctcttg atcctaagaa aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg
    70201 ggcttgattg ctgctttggc tgttgattcg gatctctatt tatttgatga gcctacttct
    70261 ggtttagatc ctttgatgga aacgattttc caagaagaag ttgaaaaatt gaaagaacag
    70321 ggcaaatcca tcctactttc ttctcatatt ctcagtgaag tcgagcgact agcggataaa
    70381 gtcgtgatca tacgcgaagg aacactcgat gaagaaggaa cactcgatga attgcgtcat
    70441 ttgactcgtt cgaccatcac tatagaaaca gaagcagaga atagtcactt agcacaattg
    70501 aacggtgtcc atgactttgt gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa
    70561 gctttcgatg tgattttagc tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt
    70621 ccaccgactc ttgaggattt attcatgaga cattatgaag gatgaggagg aagcgaaatg
    70681 actgataaat tttcacgctg gtccaccctt ttttctcaaa accttagccg tgactggaaa
    70741 aagatcctga tatggctagt cggtgtcact gctttttctg gtggttttgt ccctgcgttt
    70801 gaagaattga cttccggaca aggattagct ggattatacg aaaccttgca aaacccggca
    70861 atgattgcaa tggtcggacc aactccagct tcgaatgctg ctgagtatac tttaggagcg
    70921 atgtatgcaa atgaaatgtt gcttttttgc agtttgtttg ctgctgtgat ctctgtttta
    70981 cacgtggtga gccaaacgag aaaaaaagaa gaacttggtc tgattgaact tattcgttcc
    71041 tttcgagtcg gacgtcaagc aaactcttta gctgtcatcg ctgaaagtat tctgatcaat
    71101 ttgcttttag caatatcgat cagctgtacg atgatttttt tccaagcgga ttcgatcact
    71161 gcagaaggtg cttggctttt tgggctatct gtaggtagta tggggatttt ggggagtgcc
    71221 gttgcactgt ttgctgcaca actgatgcca agttcagcca gttcgaatgg tctgtcttta
    71281 gcgtttttag gtggacttta ccttctacga gcaataacag atgtagctga ttcctccctt
    71341 tcgatgtgga atcctttagg ctggtcttat ttgacctatc catttacaac taacaactgg
    71401 attcctttgc tttatttgtt tttgttcagt ctaatcttga taatcagttc atttgtttta
    71461 gaggaacatc gagatttgaa caccggctat atccccgaac gtgaaggaaa accgtatgct
    71521 aaacgttctc ttttatcctt gcgtggttta ttattctatc taaataaagg gatgacaatc
    71581 agttggctta tcacttttgg tttgatgggg gcggcatacg gctctatcta cgggaatatg
    71641 caacagtttt tagaaagtaa cgacttaatg aaacagatgt ttatccaatc tggcgtctca
    71701 attgaaaaaa actttacaag cacgatcatg gtagtcttga tttgtctagc tgctatttta
    71761 ccaattgcta gcatcaatcg tttatttaca gaagaaaccc acggacgtat gaatcagctg
    71821 ttcgcaacaa aaatttctag gaaccaactt tattggacca atatgttact agctattcta
    71881 tttgctatat tggcaatcct gattagttct ggtgcactag ggctttctgc tttggcttcg
    71941 atgaaggatg cgaaagacat ggatctattg atgtttctta aagccgggtt caatttcctg
    72001 cctgccgttt tagtgtttat cggaattagt ggctttctat tcggatggct tcctcgaatc
    72061 ggtaaacttg cttatatcta cttagcttac tcctttgcac tcaattactt tggcggaatt
    72121 ttggatttac cagaatggtt ttccaaaaca gctgtcccta gctgggttcc acggatgcca
    72181 aacgattctt ttgatctttc cgtgttcctt acgttatcat gtatcagcct tgcactgttc
    72241 gtccttggtt ctattggtta taggcgaaga gacttactag aaggataata aaaaagcatg
    72301 tacaatttca tgctagcctc caaatctggg tcaaaaatcc aacatttgga ggtcattttt
    72361 ttatttacaa gtcttctaac tgctctttat caatgatcca gacttctcga ttctttcttt
    72421 ttacataacc attctcctct aataatttaa attttctaga taaagtttcg ggggtcgtac
    72481 cgataaatgc agcaagttct ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg
    72541 ctttagctaa gtcaagcaga tagacagcga ctcgttcttc tactttttcc atcatcaaaa
    72601 aagttgtctg ctgctgtact tttgttaatt tctcaacgtt gattgctaaa agtttcagac
    72661 taagggcggg ataatgcagt aagagttccg agaaatcttt ttgtctcaat atgcaaattt
    72721 ccgttttttc tagtgcttcg ccaaataaag actcatttgg aacgcctagc aactggctct
    72781 ctccctcata tccgcccggc tctattactc gtaaaagctg ttcctttccg ctagcagata
    72841 attgatacac tttcatactg ccggaggcca cgatcactaa ttgaggttct ccattcggcg
    72901 caagaatctg ttctcctttt tccacatagt gatgtttgac aagtgcgtgt actttttctt
    72961 gatcttgctg tactaagtga ttgaacaaag gaacaaatga aacacataaa tgatcatgac
    73021 tcataatctc tcctccttct aaacagctat ttctaataaa gaaacataac ttcccttatt
    73081 ccaactgata attttctttt atcatagcat aaacgtcaag cgaaccagat accagcaaat
    73141 agagtggatt tctttttgtc ccttgcttta ttttccttct tttgagacct taagtaattg
    73201 attcctatca attttttaaa tatttacttt ttgaataatc aaaaacaaac cttcactcgc
    73261 aagccgagag taaggtttgt ttttcgcatt gcttatttcg tgttgtctat tgaaattttt
    73321 ccgcacctag cttttgatat agtggtgcta gttcttgtaa ggtttcagcg gttatttgct
    73381 caacgtgttc gatttcttgc ggatagaaga atagtttacc catttgaaag gttcggtctc
    73441 tcatcgcttt tttcaactct tcttgtttct ttgtatcgga caaatagtca gacaaagcta
    73501 agtggtcgtc aagttcatgt tctggttgcg gccatatgta ataatccttg atatcgactg
    73561 ttttgctcca ctctttcagt atcgcaagca actggttata cgctgcaatg ctaccatatc
    73621 gttcttcgct tttataatgt tggtacatca aatagatttg gtattgtttt tgattcaaca
    73681 aaactgctag acaggcattc tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat
    73741 gactgcgtaa attccagcca ttcgtccagc tttcgatttt aggtttgctc atgccgtaca
    73801 tatcaggcaa aagtgcagct gtctggagct gaatctcttt ccatttttgc cagtgttctt
    73861 tatattcttt cttgatttgt tcaatcttat ctgttggatg gctctctttg agctgtttga
    73921 atgcgtagat tggtcgatca aacaactgat aatctgattt tccaagttcc attgtttcgc
    73981 ctctttccgt tatcttttgt attctacctg aattttgcga tatggacaaa tagaggttgt
    74041 gaaaaagctg ttctgcatca agtcataaga acagccaaac aaaaaactgc gatgagcatc
    74101 accgcagttt t
//
LOCUS       DANKEF010000009        66073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000009 DANKEF010000000
VERSION     DANKEF010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 66073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.009"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(85..384)
                     /locus_tag="PWZ48_000655"
     CDS             complement(85..384)
                     /locus_tag="PWZ48_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2260334.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            735..1367
                     /locus_tag="PWZ48_000656"
     CDS             735..1367
                     /locus_tag="PWZ48_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2260335.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            1490..2128
                     /locus_tag="PWZ48_000657"
     CDS             1490..2128
                     /locus_tag="PWZ48_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2260336.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(2247..2319)
                     /locus_tag="PWZ48_000658"
     tRNA            complement(2247..2319)
                     /locus_tag="PWZ48_000658"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2284..2286),aa:Thr,seq:cgt)
     gene            2440..2682
                     /locus_tag="PWZ48_000659"
     CDS             2440..2682
                     /locus_tag="PWZ48_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260337.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            complement(2821..3450)
                     /gene="upp"
                     /locus_tag="PWZ48_000660"
     CDS             complement(2821..3450)
                     /gene="upp"
                     /locus_tag="PWZ48_000660"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDL2260338.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(3584..4531)
                     /locus_tag="PWZ48_000661"
     CDS             complement(3584..4531)
                     /locus_tag="PWZ48_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2260339.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            complement(4684..5928)
                     /locus_tag="PWZ48_000662"
     CDS             complement(4684..5928)
                     /locus_tag="PWZ48_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDL2260340.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            complement(6103..7128)
                     /locus_tag="PWZ48_000663"
     CDS             complement(6103..7128)
                     /locus_tag="PWZ48_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDL2260341.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            complement(7150..7329)
                     /locus_tag="PWZ48_000664"
     CDS             complement(7150..7329)
                     /locus_tag="PWZ48_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260342.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            complement(7322..8161)
                     /gene="prmC"
                     /locus_tag="PWZ48_000665"
     CDS             complement(7322..8161)
                     /gene="prmC"
                     /locus_tag="PWZ48_000665"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDL2260343.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            complement(8154..9227)
                     /gene="prfA"
                     /locus_tag="PWZ48_000666"
     CDS             complement(8154..9227)
                     /gene="prfA"
                     /locus_tag="PWZ48_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDL2260344.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            complement(9230..9814)
                     /locus_tag="PWZ48_000667"
     CDS             complement(9230..9814)
                     /locus_tag="PWZ48_000667"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDL2260345.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            complement(9920..10389)
                     /locus_tag="PWZ48_000668"
                     /pseudo
     CDS             complement(9920..10389)
                     /locus_tag="PWZ48_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            10495..11097
                     /locus_tag="PWZ48_000669"
     CDS             10495..11097
                     /locus_tag="PWZ48_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2260346.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            complement(11201..11770)
                     /gene="lepB"
                     /locus_tag="PWZ48_000670"
     CDS             complement(11201..11770)
                     /gene="lepB"
                     /locus_tag="PWZ48_000670"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2260347.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(12000..12767)
                     /locus_tag="PWZ48_000671"
     CDS             complement(12000..12767)
                     /locus_tag="PWZ48_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260348.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            complement(12764..13243)
                     /locus_tag="PWZ48_000672"
     CDS             complement(12764..13243)
                     /locus_tag="PWZ48_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HDL2260349.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            complement(13368..14105)
                     /locus_tag="PWZ48_000673"
     CDS             complement(13368..14105)
                     /locus_tag="PWZ48_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HDL2260350.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            14266..15666
                     /locus_tag="PWZ48_000674"
     CDS             14266..15666
                     /locus_tag="PWZ48_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HDL2260351.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            15889..17241
                     /locus_tag="PWZ48_000675"
     CDS             15889..17241
                     /locus_tag="PWZ48_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HDL2260352.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            17234..17932
                     /locus_tag="PWZ48_000676"
     CDS             17234..17932
                     /locus_tag="PWZ48_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HDL2260353.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(17994..19145)
                     /locus_tag="PWZ48_000677"
     CDS             complement(17994..19145)
                     /locus_tag="PWZ48_000677"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2260354.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(19158..19595)
                     /locus_tag="PWZ48_000678"
     CDS             complement(19158..19595)
                     /locus_tag="PWZ48_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2260355.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(19610..21376)
                     /locus_tag="PWZ48_000679"
     CDS             complement(19610..21376)
                     /locus_tag="PWZ48_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDL2260356.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            21640..22317
                     /locus_tag="PWZ48_000680"
     CDS             21640..22317
                     /locus_tag="PWZ48_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260357.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            22320..23447
                     /locus_tag="PWZ48_000681"
     CDS             22320..23447
                     /locus_tag="PWZ48_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260358.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(23647..24087)
                     /locus_tag="PWZ48_000682"
     CDS             complement(23647..24087)
                     /locus_tag="PWZ48_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2260359.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(24098..26131)
                     /locus_tag="PWZ48_000683"
     CDS             complement(24098..26131)
                     /locus_tag="PWZ48_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2260360.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(26133..27836)
                     /locus_tag="PWZ48_000684"
     CDS             complement(26133..27836)
                     /locus_tag="PWZ48_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2260361.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(27930..28880)
                     /gene="manA"
                     /locus_tag="PWZ48_000685"
     CDS             complement(27930..28880)
                     /gene="manA"
                     /locus_tag="PWZ48_000685"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HDL2260362.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            complement(28919..29944)
                     /locus_tag="PWZ48_000686"
     CDS             complement(28919..29944)
                     /locus_tag="PWZ48_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2260363.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(30153..31022)
                     /locus_tag="PWZ48_000687"
     CDS             complement(30153..31022)
                     /locus_tag="PWZ48_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HDL2260364.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(31726..33618)
                     /locus_tag="PWZ48_000688"
     CDS             complement(31726..33618)
                     /locus_tag="PWZ48_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260365.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(33615..35342)
                     /locus_tag="PWZ48_000689"
     CDS             complement(33615..35342)
                     /locus_tag="PWZ48_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260366.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(35358..36008)
                     /locus_tag="PWZ48_000690"
     CDS             complement(35358..36008)
                     /locus_tag="PWZ48_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2260367.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            36212..36508
                     /locus_tag="PWZ48_000691"
     CDS             36212..36508
                     /locus_tag="PWZ48_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDL2260368.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            36700..37320
                     /locus_tag="PWZ48_000692"
     CDS             36700..37320
                     /locus_tag="PWZ48_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDL2260369.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(37412..38578)
                     /locus_tag="PWZ48_000693"
     CDS             complement(37412..38578)
                     /locus_tag="PWZ48_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2260370.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(38590..38943)
                     /locus_tag="PWZ48_000694"
     CDS             complement(38590..38943)
                     /locus_tag="PWZ48_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2260371.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            39071..40093
                     /locus_tag="PWZ48_000695"
     CDS             39071..40093
                     /locus_tag="PWZ48_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HDL2260372.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            40112..40273
                     /locus_tag="PWZ48_000696"
     CDS             40112..40273
                     /locus_tag="PWZ48_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260373.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(40532..40966)
                     /locus_tag="PWZ48_000697"
     CDS             complement(40532..40966)
                     /locus_tag="PWZ48_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260374.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(41076..42935)
                     /locus_tag="PWZ48_000698"
     CDS             complement(41076..42935)
                     /locus_tag="PWZ48_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2260375.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(43053..44212)
                     /locus_tag="PWZ48_000699"
                     /pseudo
     CDS             complement(43053..44212)
                     /locus_tag="PWZ48_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
     gene            complement(44519..45943)
                     /gene="araA"
                     /locus_tag="PWZ48_000700"
     CDS             complement(44519..45943)
                     /gene="araA"
                     /locus_tag="PWZ48_000700"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDL2260376.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(45959..46657)
                     /locus_tag="PWZ48_000701"
     CDS             complement(45959..46657)
                     /locus_tag="PWZ48_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2260377.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(46614..48275)
                     /locus_tag="PWZ48_000702"
     CDS             complement(46614..48275)
                     /locus_tag="PWZ48_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HDL2260378.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            48493..49893
                     /locus_tag="PWZ48_000703"
     CDS             48493..49893
                     /locus_tag="PWZ48_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HDL2260379.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPTIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(50145..51224)
                     /locus_tag="PWZ48_000704"
     CDS             complement(50145..51224)
                     /locus_tag="PWZ48_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2260380.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(51310..52269)
                     /locus_tag="PWZ48_000705"
     CDS             complement(51310..52269)
                     /locus_tag="PWZ48_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2260381.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(52281..53792)
                     /locus_tag="PWZ48_000706"
     CDS             complement(52281..53792)
                     /locus_tag="PWZ48_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2260382.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(53814..54377)
                     /locus_tag="PWZ48_000707"
     CDS             complement(53814..54377)
                     /locus_tag="PWZ48_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2260383.1"
                     /translation="MKIVETKLYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(54383..55246)
                     /locus_tag="PWZ48_000708"
     CDS             complement(54383..55246)
                     /locus_tag="PWZ48_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2260384.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(55260..56156)
                     /locus_tag="PWZ48_000709"
     CDS             complement(55260..56156)
                     /locus_tag="PWZ48_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2260385.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(56331..57620)
                     /locus_tag="PWZ48_000710"
     CDS             complement(56331..57620)
                     /locus_tag="PWZ48_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2260386.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(57592..59109)
                     /locus_tag="PWZ48_000711"
     CDS             complement(57592..59109)
                     /locus_tag="PWZ48_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HDL2260387.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            59307..60197
                     /locus_tag="PWZ48_000712"
     CDS             59307..60197
                     /locus_tag="PWZ48_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HDL2260388.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(60270..61094)
                     /gene="nadE"
                     /locus_tag="PWZ48_000713"
     CDS             complement(60270..61094)
                     /gene="nadE"
                     /locus_tag="PWZ48_000713"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDL2260389.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(61106..62578)
                     /locus_tag="PWZ48_000714"
     CDS             complement(61106..62578)
                     /locus_tag="PWZ48_000714"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDL2260390.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(63215..63577)
                     /locus_tag="PWZ48_000715"
     CDS             complement(63215..63577)
                     /locus_tag="PWZ48_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2260391.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            63800..64552
                     /locus_tag="PWZ48_000716"
     CDS             63800..64552
                     /locus_tag="PWZ48_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2260392.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(64692..65615)
                     /locus_tag="PWZ48_000717"
     CDS             complement(64692..65615)
                     /locus_tag="PWZ48_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260393.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(65630..65902)
                     /locus_tag="PWZ48_000718"
     CDS             complement(65630..65902)
                     /locus_tag="PWZ48_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260394.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        19113 a        13537 c        11475 g        21948 t
ORIGIN      
        1 acagactctg ttcaaataaa tgaacctagc ctgcattatg agattgacta tactttttaa
       61 ttcattgaga tgaataactc gtttttataa atgcccaaaa gtaatcggtg ccgaagccat
      121 tcctcgacct aattcttttt caaattttcc tattgttgat atctccatat aaaacggtga
      181 tactggttta gacaattctc ccctgattgc taacggacgt aaactacgtt ccaaattatt
      241 tatgacttta ggataatact gacctttttc taagcctgtc tttgcgtcta tcaaaagtat
      301 acgttccttt tctttgatct ctggatttaa aacgagatca tataatttta gtaccatgtt
      361 ttctatttct tcacttttag acatcacgat ttcctcccaa aaatatattt cttcccacta
      421 cattctataa acaaagtata cgctacatct agattttttt aaatcattac tggattgtta
      481 aaacctttca atacactgtt atggcaacga tttttatttt aaaaacattc taaattaaat
      541 cagtaaaaat ttttattcta agatttttat tagaattacg cctaatttgt gtctctcagg
      601 gcaattctaa gaacgtttca agagatagcg acactatcgt tatcgaaaaa ggcgaaaacc
      661 tatctcattt tctgctaatg taagttggtt tagcttttgt taatatgact ttatgaaaat
      721 ggaggtaatt aaatatgatt tttcctaaag ttaatgaagc aagcttgaat aaaggatcct
      781 tttttcatat ctatgaaaac gaagattttg aactgtataa tatcctttca caaaaagagg
      841 tcatcgaaaa ccaaataatc tgccatccaa ttatttacaa atctattttc aagtatgcat
      901 tttttgcttc caatgatttt caaagaatgg agattaccga ctgtatattc gaaaactgtg
      961 atttttcagg ctgtatattg acaaaaagca tgttgcatcg cgtgaaattt gtcaattgta
     1021 aattaacagg tactaaattg atggaaagtt atattgggaa cacgttattc gagaattgta
     1081 aaatggacta tgtaaattta tctggttcaa acataaaaga aagtaccttt gagcactctg
     1141 tcttatcaag cgcagatttt gtagactgtt ccctcactaa aataatgttt tctactaatg
     1201 atttagaaaa tgctagcttt tttgatactg atttaaaaaa tatcgattta agtagcaact
     1261 cctttgaaat gattgaagta cgctcggaac atctaaaaaa ttgtacgctt aatcagttgc
     1321 aagcacttgg ttttgctaga aaatttttac aaataaaagt agtataatca aaatgaatat
     1381 ataagataaa tttttagcaa tacaaatatc cataaaaaaa cattgcctat tttaactctt
     1441 catgtatact gactctatct tcaaaaactg aatagtagga gttattatca tgaataacat
     1501 tttaggaaaa aaaatagaac ttaaaaaagg gatattgggc actattgtcg ttgtactcgg
     1561 tttgttaggg ttgctatttg cgccatccat tattagcatt ttttctttaa ctatcactgg
     1621 atttatagca tttacacttg tttttggtaa aaaagatacg aaaaaaatgt tctcgaaacc
     1681 tgtcgctcct gtcaaaaata tagccaaata tttttttatc aatgtagtaa tcagtgccgc
     1741 agtctcggta tttttgcaac aaattttgaa atggggttta acaggcaatc caataaatga
     1801 ggctttcagc ccgttattat ttattatttt gcctatcatg atacttggag aagaattgtt
     1861 ctcagtgtat tttctcgcta ttttttctag taagttcagc attcctgtcg ctagtttctt
     1921 gtcagcaatc atctttggat ttatccatta cagtacttat gataatggaa atattttaca
     1981 cactgtagca cacatccttt tgatacaagg ggtggcaaga ttgttattca accaagctgc
     2041 cattaaaagc aactcgatca ttacaagttg ggcagttcat gtgatatttg atttcacagc
     2101 tatattgcta gtcgttttat tctcctaaat ttttatagaa ataatacatg ctctattgct
     2161 aagaaagcat actttatttc ctttaaacaa aaagacagat gtatcaatgg attaaaaatc
     2221 cttgatatat ctgtcttctc ttttaatgcc agctataggg atcgaaccta cgacctacgc
     2281 gttacgagtg cgttgctcta ccaactgagc taagctggcc ttatccaaaa gaaattatat
     2341 actctatttg gctgattgta aagagtccga tggaaaatta gtttaatttc atgatatttt
     2401 tcataaaacg tggtataatt gatgtgaaag gcaggtttta tgaaaatggc aacttttgga
     2461 aaatttgata caactatcgt accatcggaa attggtgaaa aattcaagat caatactcac
     2521 gtaatgttca caattcacgg caagtcgcaa actggaacga tttcaaagca attgaaaaat
     2581 tccgctgttg ttgagattga tgaaactgca gaaaatgcga acttgatgac gcaaagtaag
     2641 ggtgtagtaa tcgttaacta caaagaacta aaaaagattt gaatttgacc atctctgcat
     2701 ttttgggatg gtttttttat atgcaaacga aagctgtgaa aaagctgttc tgcatcaaac
     2761 gttaaaaatg accaaaaaag gatgtgacaa aagttgtgtc acaccctttt tcgtatttac
     2821 ttattttgta ccaaacaaac ggtcacccgc atcccctagt ccaggaacga tataaccatg
     2881 ttcattcaat ttttcatcta gagaagctgt atagatgtca atatcaggat gcgcttcttg
     2941 caaagctttt actccctcag gtgctgctac caaacaaaca aatttaatgt tcgacgcgcc
     3001 acgttctttc aatgcatcga ttgccatgat tgctgatccg cctgttgcca acattggatc
     3061 tactacaaac aactgacgtg catcaatgtc ttctggaagt ttgacgaaat attcatgtgg
     3121 ttccaatgtt tcttcatctc gataaagacc gatatgtccc actttagcag ctggaatcaa
     3181 ttccaagata ccgtcaacca ttccaatacc tgcgcgcaag attggaataa tcgctacttt
     3241 tttaccagac aacgtttttt gtgttgtttc agtgatcggt gtttcgatca cgatgtcttc
     3301 caatggcata tcccgtgata cttcatatgc catcaacata gcgatttcat cgactacttc
     3361 acgaaaaacc tttgttccgc agtttttgtc ccgaataata gttagtttgt gttgaatcaa
     3421 tggatgatcg atcacttgaa atttgcccat tttttaagct ctcctttgtt ctgtcgtttc
     3481 tttttaattg taaaacaaaa tcaagtgatt gactagtcaa actcgccatt tttcctaaga
     3541 aaatccgttt ttgaaaagaa gttttcctgc ggacaagcaa agcttattct tttttcttga
     3601 tccaagaagt agataatgcc attgctgcac gcgttgcctt tgtctgatcc atagcattta
     3661 ccatgacaaa atcatggatg attccttgga agcgtacttg tgtaacagga acccctgcat
     3721 cacgaagttt gcgcgcataa gcctcacctt catctcttaa tacatccgct tctcccgtta
     3781 aaatcattgc tgaaggaaga tcatttagtt catttatatt tgctctcaaa ggcgaagctg
     3841 tgatttcagc acgctcttct tcattcgtcg tatattggtc ccaaaaccac atcattcctt
     3901 ctcttgtcaa aaaatagttt tctccgaatt catgatagga atctgtatca aaatcagcgt
     3961 ttgtgactgg ataatacagc aattgcttgg atattgggag gccctttcgt tcttttgtta
     4021 agatcgtcat gactgtagcc atgttaccac ctactgaatc tcctgcaacg attagattct
     4081 cgagatccag attttttgac ttggctaatt tattcagctg ttgcaacaca gcataatttt
     4141 gttcaattgc tgttggatat tttgcttctg gtgatcgact atattctgga aagacaacga
     4201 cggaattcgt acgaaccgca agttctctga tcaatttatc atgggtttga gcactgccaa
     4261 aaacccagcc tgctccatga atataaaaaa taactggtaa tttatccata ttcccttctg
     4321 gtcgtacgag acgaatattg agttttcccc attcgccagt atccaccatc aagtcttcaa
     4381 tatcgacgga atatttgttc acaggtgatt cttgtacttc ttctagtaaa actcttcctt
     4441 cttccggtgc tagttcatag atacgcggat gaggcgcatt tgcatcactg aatgctactg
     4501 cttccttttc taacgagata cgatcagtca tgacttttcc tccctgagat ttagtgtgaa
     4561 gactaaaatt acacctcata tactagagag catcaccgat attgtcaaag aatccgtttt
     4621 cccaaaaaat aaacagaaaa gcccaacatt gaacttttct gtttactaat ggaatttaaa
     4681 tttttatttt ttatgcaacg gaaatttttc agttaattcg cgtacacctg ttttgacttc
     4741 gtctagttga gcgttatcat cttttgcttg taatgctttg acaactagtt cagccacttt
     4801 tacagcatct tcttctttga acccgcgagt agtgattgct ggcgtgccga ttcgaatacc
     4861 gcttgttttg aatggactta gtgtttcaaa aggaatcgag tttttattta cagtgatatt
     4921 cacactgtcc aaaatgctct ctgcttcttt tccatttata cctaactcgc gaacatcgat
     4981 caacatcaga tgattatctg tcgcccctga aatgactctg gttccaattg cttgattgaa
     5041 aactttgacc attgcttttg cattggcgat gatttgttca ctatattctt taaatgcagg
     5101 atctaatgct tctttgaatg ctactgcttt acccgcaatc acatgctcta gagggccgcc
     5161 ttgaatacca ggaaataccg cactatttat tttttttgct aatgcttcat cgtttgtcaa
     5221 aatcatgccg cctcgagggc cacgcaatgt tttatgagtg gtagtcgttg tgatatctgc
     5281 ataaggaact ggattaggat gaagtcctgc tgctactaat cctgcgatat gtgccatatc
     5341 taccatcagt tttgcgccta cttcatctgc gatttctctg aatttcgcaa agtcgatcgt
     5401 tcgtccgtaa gcactggcac ctgctacgat caattttggt tgatgttttc tagctaaaat
     5461 acgtaccaca ttataatcaa taacttctgt tgtcggatca acaccgtaag caacaaagtg
     5521 ataggttttg ccactgaagt tgactggtga tccatgtgtt agatgtccgc ctgcggaaag
     5581 atccattcct aagatcgtgt cccctggttc taccaaagcg agataagcag ccgtgttggc
     5641 ttgagagccg gagtgaggct ggacgttcgc aaatttcgct ccgaataatt cttttgcacg
     5701 gtcaatcgct aaattttcaa cgatatcgac aaattcacac ccaccataat agcgatgccc
     5761 tggataacct tctgcgtatt tatttgtcaa gatacttcct tgtgcagcca tcactgcttc
     5821 ggagacaaag ttttcagagg caatcagctc caaattatgt tcttgtcttt cttcttcttt
     5881 ggcgatcgca gcccaaagat cagggtcaaa cgttttgtaa tctaccacaa acaacagctc
     5941 ctttttattt tcctacaatt ttatttttcc catcaaaaac aaattgccaa atattcgtat
     6001 ctcttctatt acgattagtt acgcttagtt tttcgttcac tgatagaaga tttacttgat
     6061 agcattgtat catactaaaa gaatgcgaca ataaccaatt gatcattttt caaaataatt
     6121 ttgtcctgct gcttttttca agcgattcat gtaagcattc cccaatccag tttctggata
     6181 cacttgtaca aaaatcaaat ctaatccaag tgttggttca tctaatcctc ttagacctgc
     6241 aaacaatcct ttagccgccg cttctactga gttgtcgtta tacatataaa cagctgcagt
     6301 atctgtccgc acttgatccg caatttcggg gcttgctatg acacctgcac ggatcttctt
     6361 attttttgcc cattgaactg cagttgacca atcgccatct ctgaccatca gaacacgtgt
     6421 atcgggtgag tagtgtttgt atttcatccc aggggctttt ggtgtttcat tttcttttac
     6481 aagatgctga tcaatagcaa caggagattc gatgactgct tcgatttgtt ctttcgttac
     6541 tgcacctggt cgtaaaatca taggcatggc tgttggatca cttaaatcaa gaacagtgga
     6601 ttctacacca attcgcgtag ctccatcatc aataatcccg gtaatttttc cttgtagatc
     6661 atgatataca tgatctgcag tcgttggact tggttttccc gacgtatttg cacttggacc
     6721 gacaagcgga acaccggaaa gttcgattac ttctagtgtt tttttgttat ccggtatacg
     6781 aaatgcagct gtggataatc ctcccgtcac aactgagggc aaagtgtctt ttttgatctt
     6841 aaatatcaaa gttaatggtc caggccagaa gttcttcaca atcttctctg ctgccggatg
     6901 aaagttgtcc acatactctt ttacttgttc aaaagaggcg acatggacga ttaatggatt
     6961 atcttgcggt ctccctttta cagaaaacac tttcttgaca gcgtttggta aaagagcgtt
     7021 ggcgcccaat ccgtatactg tttcggtagg aaaagcaaca agttctcctt ctttcagtgc
     7081 ttcggctgcc tcttgcaatt ctgtttcatc atatcgtttc gtttccacag tttatccaca
     7141 accccttttt catacattct tattttgtct gagtttatcc tcaagttttc cactgtttgt
     7201 ggataatcta tttgtcaata gtggataaac ttgttgataa accaaaaagt cgtttacatc
     7261 cttgtcaaat aagtgttttt ttataataaa aaaacaaata aaaaattttt catccacaac
     7321 ttcaaccata aacttggatc attcgttcat tgccaaaaag atcctttttt atcgccactt
     7381 ttttatctgg aaatgcttgc tgaaatattt tttttactgc ttcaccttgg cgaaatccga
     7441 tttctaaaaa aatctttcca tttgatttta gtacgcttgg tgcttcaact gctatttttt
     7501 cataaatagc taatccatcg ttctcagcaa aaagggccat tttaggttcg aatgaccgga
     7561 cactttcatc catcagttcc cattcattcc gactgatgta cggcggatta gaaataatca
     7621 catcaaattg ctgatccatt actggtccta atgtatctcc atgataaaaa ctgattttcg
     7681 ttccttcttt ctgagcattt tctttcgcta cttctaatgc ttcttctgaa agatccacag
     7741 cagatatatg ccaatttttt cttgctgctt tcaaactaat agcaattgct ccagtccctg
     7801 tcccaatatc tatcacttct aacggtacac caggcgtctc gtctaaacac cattcgacta
     7861 actcctctgt ttctggtcga gggatcagtg ttgcttcagt tacctttaac cgatgatcat
     7921 aaaaatctgc atatcctagc aaatactgag gtggatgatc tgcaagcaga agtcgcagat
     7981 cggtttcgat caaccgctgt tcttcttctg aaatctcttc gttcatatga aggagccaat
     8041 caagtttttt ccattgtttt cgttccaaaa ataaaaattg gatactatat gcttctttcc
     8101 catgatcctc taaaaaagaa gaagcccgtg acaggacttc tcgataggtc attttaccca
     8161 ttttgcatag cttccagttt tgatgtttga tcatacatga tcaatgcatc aacgatctca
     8221 tctaatttcc ctgctaaaat ctgatctaat ttttggatag ttaatccgat acgatgatca
     8281 gtcacacgat tttgcgggaa attatacgtc cggatccgtt ctgaacgatc cccagttccg
     8341 actgctgatt ttcgattggc atcatactcg ctttgtgctt cttgctggat cttatcgtac
     8401 acacgagcac gcaagatttt cattgctttt tcacggttct tgatttgaga acgttcatct
     8461 tgcatagcaa caacgatccc tgttggtaag tgagtcaaac gtacagcaga tgctgtttta
     8521 ttgacgtgct gtccgccagc acctgaagca tgatagatat ctgtacggat atctttgtct
     8581 tcaatatcga tctctacttc ttcagcttct ggcatcacga caactgttgc ggtggaagta
     8641 tggatacgcc cttgcgattc agttgaagga acacgctgga cgcggtgcgc accactttca
     8701 tattttaatt tcgaatatac gttgtctccg gtgatcatca tgatcacttc tttatatcca
     8761 ccgattccgg taacgttagc ttccatcact tctgttttcc agccttgtgc ttcagcatac
     8821 ttttggtaca tattgaaaag atcgccagcg aataatgctg cttcgtctcc gcctgctgct
     8881 ccacggattt ccatgatgat atttttatcg tcatttggat cttttggtaa aagcaagatc
     8941 ttgatttttt cttctaagac ttctttttct ttttttaatt cagataattc ttcttttgct
     9001 agttcagcca tttcatcatc cagtttttca ccaagcagtt cttctgtatc ggtgattcct
     9061 tgtactactt ctttataacg acgataaact tcgactgttt cgcgcgtgtt tgcttcttct
     9121 tttgaaagct gcataaaacg ctttgtatca gaaatcactt ctgggtcact taaaagttct
     9181 ccaagctctt cataacggtc ttcaattgct tgtaattgat cgtacatgct tattctcctt
     9241 ctgtaatcat tgatgggtga aagtaatgtt ttcgacaaac ggggtaataa gcttcatttc
     9301 cgccaatttg cacttgatcg ccttcataaa cagggtttcc atcaatataa tgtaaattca
     9361 tgattgcttt tttgtggcag aaccaacaga ttgttttcat ttcttcgatt ttgtctgcat
     9421 aaagcaataa atattttgat ccttcaaata actcgtttcg aaaatcattt ttcaaaccaa
     9481 atgccataac cggaatatcc aattcgtcca caattctcgt aaaagcaatg acatgtttct
     9541 tttttaagaa ttgcgcttca tcgatcagca cacaataggg tttgctttcc attttctgaa
     9601 ttacgtcaaa tacgtctgtt tcttcaaata tcggcatcgc attacgtttc agtccgattc
     9661 ggcttgaaac aacgcctaaa ccatctcttg tatcaatacc gcttgtcatt aagacaactg
     9721 gcttattttg ttcttcataa ttatgtgcga cttttaggat ctcgatcgtc ttgccgctat
     9781 tcattgcgcc atacttaaaa aatagttgtg ccatgctctt tttcaccttc cagtttgttc
     9841 ctatggtatt atacttgatt ttcagcattt tttacagtga gaattttcat caagcacaga
     9901 ttagtattaa atctattgct cacttttgtt ccaatttttt gacaagaact aaacatgggt
     9961 tccattcatc ccataaattt ggaaaaactt ccaaccttga aaatcctaat gattcgtaga
    10021 aacaaatagt ttgatcatat atagagtagt gcccctcgtc tacagtcttc acttgcaaat
    10081 atttgtaatg ttttgctgcg taaccttctg cttctttaaa aagttttgtt cctaatcctt
    10141 ggtgatgata gtcttttttt attcccatac aatagaatag atttcggcag tatcttcact
    10201 tgtctgtgaa agagtgataa agcctaaata ttcttctttt cttttttcac accataaagg
    10261 tagtttactt gattcctcga tatatttttc cgttgattca ggaagcccaa accactcagg
    10321 caagtctagt aatatttctc ggacacactt tcctttttcc tctctatctt ttacaaacga
    10381 tatcggcata ttctttctag attctccctt tttgaattga gtatagcatg ctttctttct
    10441 tttcacgtgc tataataagt taaaatttca aagttttacg taaggagcta tttgatgaca
    10501 aaaaatattt ttgattccat cgctaaccat tatgattccc ctgaccgtca agcattagcc
    10561 aagattattc gcgaagaact aactacttat ctaccgcgcg acagccacca aaaagtcctg
    10621 ctggattacg gaggcggaac tgggcttgtt agtttaccgc ttgcggaaag attcaaagaa
    10681 ctgatcattg ccgatgcttc agaaactatg ctgaaaatgg cagaagaaaa gatccaagca
    10741 gcagatctga aaaatgtccg aacgatccac gctgatgctt ctgtcgaatt tcctgctgtc
    10801 caagctaatc tcatccttct atcacttgta cttttacaca ttcctgatac ggagaatatt
    10861 cttacaaaac tttatgaaat tttagctcct ggcggtcaat tgatcatcgt agactttgac
    10921 aagaatgaac aaattagtca tccgaaagtc cataatggct ttagccaaga agaattgaat
    10981 aatcgattgg aaaaaacggg ctttgtctct gctactagtc ataccttcca tcgtggggaa
    11041 aagctattta tgaacaaaaa cgcttcgctt ttcttatcca tcagccaaaa agcttaaaat
    11101 gagattgcaa aaaaagtgag caactccaag aaataagaag gaattttcgg aaaaagaagc
    11161 tgtgacaaaa aacatgtcac agcttctttt tattttttca tcacatgatt ttaatttctg
    11221 aaagaggcca ataacggaaa acaacttttc cttggatcat atctttgtcg actaacccaa
    11281 acattcggct gtcttttgaa atcaagcggt tatcgcctag aacaagatac tttccttttg
    11341 gcacagtata ttcaaagtca ctcgtaaagg tggatgaact ttctgcttgt gcttggaatc
    11401 ctgagctata agcatattct ccttgtaact tatcttctga aaattctttt ttgaactcat
    11461 ccagataagg ttcatcatac tttttgccat taatggtcaa ctggtcattt tccatcttga
    11521 ctgtatctcc aggcataccg atgatccgtt taacaatcat ccgttcttca tcccccggtt
    11581 cctttgtagt aataatatcc attcgttcat agtttgagat cttagatgaa atcaatcgtt
    11641 gtccatcact taatgtaggg tccatcgaat gcccgtttac agtaactggt gtaaacacgt
    11701 acactctagc taataaaata gccgctaata caaggatcaa aatcccccaa tttttcaaaa
    11761 attctttcac agcttacctc ctacttgtat tttcctcgtt atttattacg ttttttcaca
    11821 atacttcgtt gtactttctt tattctacaa tgtttataaa tgaaaggaaa gaataagtga
    11881 tgtgttcgtc ttatctttct cgttatataa aaaaggaacg tgacaaaaga cgtgtcacat
    11941 cccccttatt ccgaataaac gtttttatcg caaccactga tacacggcgt ttcatcacat
    12001 tatgaatgtc ttcttctttg gctacgacta taaaccactg gttcttcctg cgactgtgcc
    12061 ttttttgcaa tcaatttcag acgataaggc tcatgatagc gtaacccgtc tttgacaaaa
    12121 tcaccgccga cgatgaacaa tccgacaggt tccaatgtgt cttctgttga gtcaagtcga
    12181 atggtcccta ctttttccat gtccgagtag cttcgtccaa tatttagaac atactctttc
    12241 tctcctgttt tcttgacata gtaataagga tacaatgtat ttctatcgac cagaaatcca
    12301 tccatgcgac cgagttcatt tgttttgatc tcaccatttc caatcaaata ccgcatattc
    12361 tcggctaatt tcaattgata atcttcgtca taagaaacca tgacctcttg tgtattcact
    12421 ttatcgatcg aatttccatt atctatcgat tcttctgtct ggatgttgaa cgcttcatca
    12481 ggtaaatact tcacaaaaag ctgagtgaca tcggctttcg tgtttttatc taaaaagtaa
    12541 taaaatacat ttaaaacagt aaaataaaca agcactagaa atgcaagtcc atataagaaa
    12601 aattgaagat agttttggtt ttgccaaagt ccataaacaa ttctcaataa ataaagcgtg
    12661 ggaatcacac ttaaaatcgt atatccacgg ttaagatatt taggactgat gtctaaatag
    12721 ccaaggaatt tatgcacccc gttgataagg tctagtaaaa atgtcattga gaatcacctg
    12781 cttgctggtt atacgtttgg gtatttcctg aatcttggtt ttgctgtgct gtacgtgctg
    12841 catcatcatt ttgcgtgtta tcatctacag tcgtactgct tggtatcgtt tcggagctag
    12901 cacttgtatc cggacttgta cttggattgg ttgaatccga tggagtgcca tctggtaccg
    12961 tattacttgt ttcgcttgag ctgtcagttg tatatgtctc atcatacgac gaactagttg
    13021 ttgtatcgtc ccatgtactt gtcgtagtgg tttcattcaa actttccgtt gttgttggtt
    13081 cagatgtcga gactgaagta gttggtgttg tttcttctac agtactagaa cttgaacttg
    13141 gtgcacggta gctggaacta ttcacaatac ctgtagtagt cgcgacaatc gtcgagattg
    13201 ttaatacagc gattggcttc aaaataggtg ctacttttcg catcttatct cttccttcat
    13261 tgaaatttat tcttgtttcc ttactttact acggacaaat tatacagtct accttatttc
    13321 taccataaat tctttatttt tttcttacgt ttttccgaaa gatttcttta attttcctca
    13381 aaaactacgg ttgatgtttt taagtaagct tttgcttccc gattgatttc tttcagatcg
    13441 catccttgct gcttggcagc gaacacgcaa ccacaataac attggcgata aatatcatac
    13501 tctttgcaca tttgcaaaga gcgttcatag cctttgtttt tcttgaaatc acttggcaag
    13561 tattgtgtcc cgtaaatctt ttggatatcc attccgatct gattgatcaa agctgcattt
    13621 ttctttggcg agatggtcag ggcacttcca aagtaatcgt atcctagttc ttgcgctttt
    13681 tgcgcaacaa gatctaatcg catattgaag catgcactgc agcgagcacc accttctttt
    13741 tcgttttcaa gatggtttgt taaaaccatt ttggtgaatt catttggacg gtattcatca
    13801 acgattagcg caacatgatg gtcagttttc tcattaaatt ggtctacaaa tcgtttttgt
    13861 tcatgtaatc ttcgcaaata ctcactttta ggatgaatat tcgaatttga aaaaaataac
    13921 gtgatctctg catattgaga catgaattcc aatgtgtgcg tactgcaagg agcacagcat
    13981 gtatgtatta atatcttagg ctttacttct tctttctccc attgcatgat catttttttc
    14041 agaactgtat cataatttat tttttgattt ttcatttttt ctacgatttg ttgagcatta
    14101 atcaatttgt tattcctcct ccttcttttt tctaggattt tccttattat aagccatttc
    14161 tattttaatc gaaagctgta ttgttatctg tttgataatt tctttgagta ttcctaattc
    14221 cccttgtata ctggtagaaa cgactattgt caggaggata gtactgtgca tgttgattta
    14281 ttctttcgaa ggctgcagcg gcgaggacag aagtttgctg ccaatcattt ctcatcgatc
    14341 caaatcatcg ttttttatta tatattgatg acggtcttat cactcgttct attttatatg
    14401 ccgatttttc gcgaaccaga tagccatgtt tcttttgtgg acatgctgtt catggcaatc
    14461 agtacagtga gtgtgactgg actatcgact tttgatatca attctgtgtt caatgataac
    14521 ggtattatac tgctggaaat attatttcaa gttggtggtt tggggatcat gatgatttct
    14581 actgcttttg tcattttttc taaacgacgg atcacactga aacagcgaca gctgatcatg
    14641 acggatatga atcaaccgcg tttgtctggt atcgtccgac tgattcggat cacttttgct
    14701 attttaatct ggtttcaatt actttttggg acattctttt ctatttattt ttattacaga
    14761 ggctattttg atcgttggcg tgatgccgtt ttttacggtt tttatcaggc gatatcggct
    14821 gtcacgaact ctgggtttga cgtcactggg gattccatca agccctttgc acatgactat
    14881 tttttcttga tcctgatcat gtttttgatt tttgtcggag gaattggttt tcctgtattg
    14941 atggagtgcc gggaatggct attatacaaa cgttccaatg cgaagcttcc gtttcgtttt
    15001 tccttgttta ccaaattagc tgtgttagct tttgttatct tgtttgtttc gggaactgtc
    15061 ttgatttatc tattggaaaa ggaccattta ttccaagatt ccaatatgag tgtcaaatgg
    15121 atcaattcga tgttttattc gattacgact cgtaatgctg gtctgcagat tcacgatttg
    15181 ggtgattttc aaatcacaac actgatcatt ttttctttac tgatgtttat cggatgcagt
    15241 cctagttctg taggcggcgg gattcgaaca actactgtag caatcatcgg tttgtatcta
    15301 tattccttcc tcaaaagtga agataacatc aatatttttg gacggagaat cgatcaagat
    15361 gatgtgcgaa aatcagtcgt tgtctttatg ctttctcttg gtatgtgttt cttttgtatc
    15421 gtttttttat ctgctacaga agaacaaacg ctgatctcga tcatcatcga agtaacttct
    15481 gcgtttggaa caactggttt atctttaggg attaccggag acttgtctgt tgtgggcaaa
    15541 atcacgattg ctacactgat gtttattggg cggatcggaa tgctatatac attgatgtta
    15601 ttcgtgccaa aagaaacacg agatctaggc tatgaatatc caagcgagaa aattattatc
    15661 ggttaaaatg ggaatatagg taagagggaa taataaaatc acagcagctt gaaacaatca
    15721 ttttgtttca agctgttttt ttgttaaatc atgcgatttt gtccgatttc atgcactttc
    15781 agccgtaacc ttttcttaag aataagaaaa gcaatttaac ctttttttca acatatggta
    15841 tgattagttg aatttattcg tcagcaaatc gtttttggag gttacataat gggaatacgc
    15901 agtcattttg cgattgcagt tggaaaaacg tcgcaatggg cattaaaaac tttttttaaa
    15961 ggcggatcaa gtttaccagg taaactagcg ctgaaagtcg atccacatat actagatagt
    16021 ttggcaaaag actaccaagt cgtcgttgtc accggaacaa acggtaaaac attgactacc
    16081 gctttaacag tcaatatttt gcgccagcaa ttcgatgaag ttctaaccaa ccctactggt
    16141 gcgaatatgg tacagggaat tgtatcgact ttcttgcagg caaaaccaaa aaaaggacag
    16201 aaaaagtttg ctgtcctaga aatagatgaa gcaagtttga gcaaagtcac agaatatatg
    16261 aaaccagaac tgttcttatt tacaaatatt ttccgtgatc aaatggatcg gtatggagaa
    16321 atctatacga cctataaaat gattgtagac ggtgccgcaa agtcaccaaa tgcaacgatt
    16381 atcagtaacg gtgattcacc aatcttcaat tctatcgaaa cagtcaatcc tagaaaatat
    16441 tatggctttg accatgaaga agatcgtgaa caaatggctc attacaatac agacggtgtc
    16501 ttatgtccaa attgtcatca catcttgcat tataaaatga tcacttatgc aaacttaggg
    16561 aaatattact gtccaaattg tgacttcaaa cgtcctgaat tggattatcg attaactgat
    16621 atggtacgga tggacaatgt gtcagcagat ttcgtgatcg atggtcatga atacggaatc
    16681 gaagttggcg gtatgtataa tgtctacaat gctttagcag ctactgcagt ggcagagtac
    16741 ttcaatgtcg atcctgataa aatcaaacaa ggtttaggat atgacgagaa agtattcggc
    16801 cgacaagaag tcatcaatat caatgggaaa aaatgtacat tagtacttgt caaaaatcct
    16861 gtaggtatca atcaagtaat cgatatgatt ggtctagctc cttatccatt ttctttagtg
    16921 gctttattaa atgccaatta cgcagacgga atcgatgtca gctggatttg ggatggtaat
    16981 tttgaaaact ttgccgatat ggatatccca gctgttattt ctggaggaga ccgtcataca
    17041 gatatgactt tacgtctaaa agttgccggg attccagaaa ataaattgac agaaacaaaa
    17101 gaattaccag aagtaatcga aaaaatcaaa gaattgccaa ctgagcatgt ctatatttta
    17161 gctacttaca ctgctgtatt gcagcttcgt aaagaactag ctgctcaagg atttatcaaa
    17221 ggaggaatga accgtggcta attatgaact caatatcgcc cacctttatg gaaatttgat
    17281 gaacacatat ggtgataacg gaaacctttt gatgttgaaa tatatagcag aaaaaatggg
    17341 ggtttcttgc catacggaga tcgttagtat acatgaacct tttgatgctg ataaatatga
    17401 cttggttttc tttggcggtg gtcaagactt tgagcaatta atcatttcaa aagacatcca
    17461 gaaaaagaaa gattctttga tcaactatat cgaaaatgat ggcgtcatgt tagccatttg
    17521 tggtggctac caattgctag gtcactacta catgggagct aatggagaaa agatccaagg
    17581 aatcagtgca ctggatcact acacgttaag tcaggaaaat aaccggttta tcggtgatat
    17641 cgttatccat aacgaagaat tcaatgaaac ttattatgga tttgaaaacc acaatggtcg
    17701 gactttctta ggtgaaggtg aacgaccttt ggggaaagtg gtacaaggtc aaggaaataa
    17761 tggtgaagac caatctgaag gtgtcattta ccgaaatgtc ttcggttctt atttccatgg
    17821 acctatcctt gcaagaaatg agaacctggc agctcgtttg atccgcctag ctttagaaca
    17881 acgttacggt aaagaattgg atctgcccta tcttgaagga caaacagcct agttattcga
    17941 aaaaggggaa tgtgacaaaa atttcgtcac attccccttt ttcgtatcct attttaagcg
    18001 acttgcttgg cataacggtt cacgttttgc ttgatattcc cgattgtttt cggatcattg
    18061 attcctgtga cttcttgaat caattgttcc aagttttcat ggatcttcat ttcatgcaat
    18121 tgacggcttt gttcatcttc aggatcatag taattaaaaa taatccccat tgttgccgtc
    18181 aaatgtgggc aagtcaagtt atgttcttgt agttctctca ctggacgtgt aaatctttct
    18241 tgataaccta acttacgtaa tggtgtccga gcgacacgtg tgatcgcatc agagatattt
    18301 ttgttttgga agcgatgaat gattttttca atatacgcat gatgctgctc tgcatcaaac
    18361 ccccatttgg caatcagcaa acttcctgtt tcctgcaaaa ctgattttaa ttgtgccaca
    18421 actaacgcat cttgcattgc ttcatcaatg gtctggtaac ctaacaaggc acctgtataa
    18481 gcgacagtcg catgaccagt attgacacta aatagtttgc gttcgatata tggttctaga
    18541 tcatctacat ataaaacccc ttttaattgg atttttttat tttttctttg gctatcgtca
    18601 atgacccatt ctttgaaagg ttccacttgg acgaataaag gatcttcatg gtgttgtaat
    18661 gggacgattc gatcgactgc tgcatttgga aagccgatat atttgtcagt aaatgatgtt
    18721 tccggaagat atttatacac ttctttttct agaaatgctg aaccgccaat catattttca
    18781 caggcaatga tatctaaaga tgttgtgctt ccttcctttt ctctttcttg gatacctttt
    18841 gcaatcaact cagcaataaa aggtaagata ttcgggccaa tggcggtagt aactaaatct
    18901 gcttcagcga tttcttggat tactttttct ggatttgttt gattgttgat tcctttgaca
    18961 ttttttacag tgattttttc ttttgattca tctgctaatt cgatttcgta ttcttttctt
    19021 tcatttaaag catcaatgat cgtgtgattc acatctacga aagtaatctc aaatccatta
    19081 gctgccaaaa cctctccaat aaatcctcga ccgatatttc ctgcaccaaa atgtactgct
    19141 ctcatttttc tctctcctta agcaattgct agattttccg ttacttcttc tttgctgagc
    19201 gcgtctgcta gctggacaac attgtccata tcactgcaat aaagtgcgat ttgctgtaca
    19261 agttgcaaat gctcatctcc gactcctgca ataccgaaca aaacagtcgc aatcttttct
    19321 tcttcttcgg taccaaaatc tacaccatct ggtacttgaa cgacacagat gccggatttt
    19381 ttcacatatt tttttgcttc atctgttcca tgaggaatcg cgataaaatt ccccatataa
    19441 acagataaca ttttgtctct atctaccatg gcatcgatat attcctcatc aacgcaccct
    19501 gcttctacaa gcatctgacc gcaatagcgg atcgcttctt ctttggtttt aaaagatcga
    19561 ttgagctcga tcatgtcgaa tggcaaactt tgcatgctgt tttcctccct taagctgtct
    19621 gtttcaaata ttgtgcaatc gattcgtatt tttgcggatt gaccatgctg ttgactgata
    19681 aatgaatgga ttgaggtgca gccatctgta ctttttccgc taaactttct ttggctacga
    19741 tcagtgaatc aggttcatct tttaaatctt ggatattgac ttttcttaac gggatagtta
    19801 aatggttctt gtctaatata tctttgagca cggtcatccc cattgttgct gatccggaac
    19861 gatcatcgtg gacaagtaga atctctttga tttgtttgat gtttgctgaa agaaactctt
    19921 ctgctggctc attctcaatt tcagaaactg atgttttctg cttgctgctt aattcttgaa
    19981 tgatttcatc atatcgaggt gaattcaaaa agttttctac tgagacaaaa cgcgcgctcg
    20041 gtgctttttg ttttgctctt tcttgtagtt cgacctgagt aacgatcaat gtattcgagt
    20101 catctgtcag ttggctgatt gcttgattgg tcaccgtttg aggcaagtct gcttcctgta
    20161 ctttttttct caagatcgaa gcgcccatcg cacttgatcc cattcctgca tcacaagcaa
    20221 aaatgatttt ttgtacgcca gagaatgtag ctccttgagg aataccttgg gcacctttgg
    20281 aagcttgctt agctgccttt gtctcttgaa cagatttttc gaagtcttca tttgtatctt
    20341 tcgcatccgc tttcaaaatt actgcagaaa taccaaacga aacagctgcg gcaacaatca
    20401 cgcccaaaat aacaggaagg taagaattga acggtgtcat cgctagaatc gcaatgatcg
    20461 accctggaga agctggcgct cttagtccag catccatcaa ttggaaaatg aaacttcctg
    20521 acattcctcc gcctataact gataagaata ataacggttt catcataacg taaggaaaat
    20581 agatttcatg gatcccgcct aaaaagtgaa tgatcatggc tcctggtgct gaagattttg
    20641 ctgctccttt gccaaacaaa gtaaaagcaa gtaatacgcc aagtcctgga ccaggatttg
    20701 cttcaagcaa gaatagaata gattttccag cctctgctac ttgttcagtt cccaatggtg
    20761 tcaaaatccc atgattgatt gcattattca aaaataagat ttttgctggt tcaataaaaa
    20821 tacttgtcaa tggaatcaaa tgggcagata aaatgatctc tacacctttt gccattgctt
    20881 gagtcaatgc atcgacaact ggaccaatcg cagaaaaacc taataacgct aatccaaaac
    20941 cgataagacc tgctgaaaaa ttattgacta gcatttcaaa accggatttg atccgttttt
    21001 ggaatagctg atcaaatttc ttgatggcat atccaccaag aggtcccatg atcatcgcgc
    21061 ctagcatcat tggaatatct gttccgacaa tcacacccat cgttgcaatt gccccaacaa
    21121 ctgatccgcg gtcaccagca attacttttc cgccagtata accgatcaat aaaggaatca
    21181 gataagttag cattgggctt accatggtag cgaaagcctc atttggtaaa taaccatctg
    21241 ggataaatag agccgttaat acaccccagg caatcagtgc tccaatattt ggcatgacca
    21301 tgcttgataa agtactgcct aatttttgta ccctcgcttt tagcgaaatg ttgtttttgg
    21361 gaatcgtccg ttccattttg tctctccctc tctccttttg atactctaat tgtatccgct
    21421 tactaaaaag agaaatagtc gtactttggc acgaaacttt gtgccaaagg tttgagtatg
    21481 tcagcaaaat aaaaagattt cggtatcaga aaacttcaat tttaaataaa actgttattt
    21541 taattttttt ataatatgca aacaataact tttgttagca tttaatttag atgttttcca
    21601 ttctattatg ttattaaatt ttatattgga ggcctatcta tgacattact gatcaaaaac
    21661 atgaacatga aacgtggtaa aaaacgtgtg attcgacaat tgaatctttt agtagaatca
    21721 ggagaagtag ctgctttgat tgccccgaac ggatttggca aaacaacttt gctgaatggc
    21781 attgcccaac tgctgccaac aacttatgat caattgtccc ttcacaacat ctctcctact
    21841 gcttatacag attatcgccg gtctttcttt tttctagaat caacgaatca gttatccgac
    21901 tatctatcgg ctgttgatta tttgactttg atcaaatact attggcagag caaagtagaa
    21961 tgccaaactg ttttccaacg attgaagatg actgagtacc ttactttgcc gattggaaaa
    22021 atgtctctag gaatgaaaca acatgtcttg cttgctgctt acttgatcag tgattcatct
    22081 gtcatgttat tcgatgaacc gttgaacgga ttagacccag gaagtattga tatcttgaat
    22141 agcctcatca gacaatggca gaaagagggg aagacgattc ttctttcttc ccacgctatc
    22201 gcgaatatcc aatcgatttg ttctaaagca tgttttctaa aaaatggaga aatcgtcaca
    22261 gaatcctcaa atatggaaga gattttagct atttatcgta aattatatga ggtgtaacta
    22321 tgtctttact tacatttgaa cgcaatctct tactaaaaaa caaagtaaac tttttatttc
    22381 ctctattgtt ggtcgtgctc tttgcattcc cacttttttt tgatcataaa cttgcttata
    22441 cagaattcga tgaactgaat cataactatg aagagatgca acgtttgatt gaaacgttga
    22501 aagaagatga gaatgagaaa gaatttgtgg aaagtttaga gaaaagcaac aaactgatcg
    22561 aagcaatctt acatgcaaaa aacacaggga atgtccagca aatggtcgaa gcaacttatc
    22621 attatgaaaa agatatactg gatcgactga tttctggtca aagacaagga attccaatca
    22681 ttgaacaaca aaaacgtgtc gagttgctcc gttatatgaa agaacatcag atccaaagat
    22741 attctatttt tgacttgcca gcgcatctct ctttagcaaa ttactacgaa aatatttttt
    22801 caggaatgat ttctagtttt ttgattttat gtataaccgc cttgtttctt tcttccatca
    22861 tctcttatga aaaaagaaag caggtaatct ctctagtcaa tctattaccg gatagtatgg
    22921 taaagaaaca tagcatccgt tttaccattt attatggtgc agctatgcta agtctcgtga
    22981 tgccattttt gatcgtctct atactagtaa taatcaaaaa tggtttggga gacttccgtt
    23041 atcctgtagg aacgatcatt ggtcaggaga tacgaatcct gccgatgtat gagtatcttt
    23101 tccagagctt tctgttcctg ttgttgtggg tgctcttttt aagtacaatc agttttcttc
    23161 tatctgcttt atttgaacat tctctagtca atcttttagg tacacttctt tgtcttttct
    23221 tagcagagta tcgcttgttt tcttcgattg gatggataga atcaatcagt cattacttgc
    23281 caacttctta tgttgatttt caaaatgtga tcataggagg agatattttc tcgccactag
    23341 cttcagaaca ggttactttc atgaatggta tcttgacact tggtatatgg agtatcgttt
    23401 tattattcat aggtatgggc actatctata taaaaaagag ctattaaaac acaaaaataa
    23461 taggggcggt gacaaaaacc ttgtcaccgt cccctatttc aaataaacgg ttttctagaa
    23521 taaatagttt tcaaaagctt gcatccgcag taataagaac ctcccaacaa aaatagctcc
    23581 tcttattttt atcttggttg aagttattac ttgatgcaag gcagcttttt ttactgtgaa
    23641 cattggttat atatgtccat gttcaaaaat catttggact tcttcaatgg tttccgcatc
    23701 actgagcgcc attacttggt cgatgtctga acagaaaaag gctaaatctt ggagcaccgc
    23761 taactgatgt tcttttttta aagccactac aaataagagt gtcgcgattt ttggttcctc
    23821 ttttgttcca aagtccacac catcaggcac ttggactaaa tagattcctt cttctactac
    23881 ttcttcatct ttgtcagtcc catgtggcaa ggctacgaaa ttcccgatat agacactcgc
    23941 tttttcttgt ctttcgatca ttgattggat atatgccggt ttgattcccc cttgttgggt
    24001 catccactgg ccgatctcgg tcaacgcttc tttccaattt tgataggatt gatcgagcaa
    24061 tattttttct gctggtagct gtatcatctt ttcaccttca tttctttatt tcttcaatca
    24121 ggattttcgc taacaactga taaacgattt ctttatttcc tgagcggaag atctcggtgt
    24181 tcaagtcatt catgatgatt gctccactga tctttcctaa aagaatcccg tctttttctt
    24241 ggattggcga aggcgccagc ataactaaca tacgggacaa atcgactggc tgtctatcca
    24301 tcccttcaat ggtcaacgga tgtttcaaat tgaaaataca aaagatcggt ttagaaatca
    24361 atgagctgga tgtatggaat aaccccatat ttgtatgagg aataccgata ggtgcttgtt
    24421 catatcgctt aagcagccgg cgatgaacca cctcttcatc cgttactagt ccttcaggaa
    24481 aataaggaaa taagctgcga atcgtttcgg aaatcgtctc aggattatcc agttcttgga
    24541 taaaaaaacg accgagtaat tgattgattt gatccatgaa atgcattgtt tcctcataag
    24601 tatcttctat catccactcg ttattgctgt tatacgtaat tttttgcctt tttttcttct
    24661 caatatgcga aaaagcttct tttagctgct tgacttcatc ttctagcagt agcggggaga
    24721 ctaaaatata ttttccaccg tatcctggta aaatagatgt agaaataaca acatcatatt
    24781 cttcttgtaa atcgattttt tttaaatcag caatccgata gaaatgaatt tgttggatga
    24841 agggaaaata ccttttcaat cgtagttcca acatgctagt agaagctaat ccacttggcg
    24901 aaaatcctgc aatactgata ttgctttctt tcggacttcg ttccaatgaa ttcgcaaaat
    24961 gaaggaccat ataagcgatc tcttcttcgg ataatttttt ttcttgaaaa atccgctcta
    25021 ctgctttacg gattgcattc gcaacttcct cgtattgaac catgattttt tctaaaatcg
    25081 gattggtcag tgtctcttct tgtaaaacgg ctcttgaaaa cacaccagat aaatgagtca
    25141 acagcatttt gtacaaagtt tcatcttcat aaaaatggac attcgtctct tcacttactt
    25201 cttgaatcag ttgttttact aaataagcaa gctgtgtatc gaacgtctct tcaaagaaat
    25261 tctgatcaaa tgagttcgtg aagtcattta ataatcctgc aaaaaagacg atctcattta
    25321 ctggataaag ttgttttgtc tcagaagcga tccgtgaaaa caatcgttta gctgtctgta
    25381 gtaattcttt gtttgccaga ctattatatg tctcatttgt cagtaaatga tttttcgtca
    25441 cacgctccat cgtcaatgtc agcatcaaaa ccaaatgttc aagctttcta tcacttagat
    25501 tggtcttttt ttggatcaat tcttccccga ttactttgcg tgcaagagat agatgttgcg
    25561 gatcaacgaa tcgaaaaaaa taattcagtg atgcatcgaa agaaatacta tgaaaaagtt
    25621 catattcatt gatttctaat tctaatacat tcgctgtcag aagccggcgg tatttttccg
    25681 gtccttcgat ctcgtatccc tggttccgaa tgatttccaa aggcagttgg cgaatacttt
    25741 cttccaactg tttgatatct gcataaaaag tggtattact gatgcgatat gattccatga
    25801 aatgagcaag actcaacggc tctgtcgtca gtaacaactc ggctaaaatc gcgtgttgtc
    25861 gtttagcagt ggataagtca ttacttttcc catctgtatt ttcttttaat tgctctaata
    25921 cttcttctga tgcctcaatg acaaatgagc ctctagctgc ttttttcaaa gaagcattta
    25981 ccgatttcaa tgagacttca agattatcta attcgcggta aatagtccgt ttactgacct
    26041 tcaataaatt catcattttt tggatagata caggtgacgg gctactgaga aattctgcta
    26101 ataattgttt ttctctgggc gataaataca tcttatattt ctcccttcac gagtttttcg
    26161 tattcattcg tagctaccca atgttcaact agaactagcc gagggatctt ttcttctagc
    26221 tgttcagtta cagctagttc tctttgtgcg atataaatcg tttcttcttg gattttgatt
    26281 ttttctattg attcttttga gataacaaac tgttttttcg ctttttttgc taattgactt
    26341 aaaatcgtca tgcccatagt ttgtgagccg cgtctgttgt ttttatacaa aaatacaata
    26401 ttagcaggcg acgttttttg ttctgtgata tctgctatct cttttttttc ttcccgcttt
    26461 ggaaattgga tcgcttcttt tattcgtgac agcaagatat ctgtttccaa aaagtgatcc
    26521 agtacataga tttgactttg aggtactttt tctcttgcaa gacccgctag ttcatgttgc
    26581 acgataacca gctgatttac ttggttattc aattcataga tagagctgta tccgacagaa
    26641 agatcgattt tttctgctgc taatttcttt ttcagcaggg cggctcccat agcacttgat
    26701 cccatacctg agtcgcaagc aaaaatgacg gaatgaatga tagatggttc caagatttct
    26761 cccctttctt tgaccgtttt gttctctttt tctttatttg gtaatttcaa tagtatcgct
    26821 gcacaaatga aggtcacaaa tgtactgatt gctatcccta tacttacccc agctagttgt
    26881 ggataaggcg cattggcaaa aatcacgagt agtgacccag gcgatacagg tgcagctaat
    26941 cccgtatcta accattgaaa tatcaacgtg ccgcttgccc caccagccat tacagctaaa
    27001 aatagatacg gtcgcagcaa gacaaatgga aaatagattt cgtgaatccc gccaatcgca
    27061 tggatcatca atgctccact cgcatttact ttttcctgtt tttctgcaaa acaaaggata
    27121 gaaagtaaga tccctaaacc aggtccagga tttgcctcca tcaaaaataa ggaagagtac
    27181 ccgactgttt gtgagaattc gattcctaaa ggtgtcaata caccatgatt gattgcatta
    27241 ttgaaaaata gtacttttag aggttctaag atcagactga caagtggtaa cagattcatt
    27301 gtgactagcc aagacacaac ggaggcgagc aacaagtgaa cactatttaa aactggttca
    27361 ataaaaacga ttccaattat acaaataacg ctaccagtca gtcctactag taggttgctg
    27421 acaagcattt catatccagc cttgaatttc acatggaatc tagaaacaaa ttgttcatat
    27481 acgatgccgg caagcggccc gataatcatc gctcctacga tttgtggagc attggaacga
    27541 gcgatcaacc ctaaagaagc aattgcaccg gcgactgccc ctttttgtcc ccctaccgat
    27601 ttcccgcctg aataactgat caagatgggt aaaagcagct ggatcatcca atggcatacc
    27661 tcttctaatg tggtgtgcaa atcgccagac acaccttttg ttgctaatga caagattccc
    27721 caagcgataa acagacttag atttggcatg atcatttcat ttaagtgatg tccagctttt
    27781 ctcagttgtt tcatagacac gcgactgctg ttgtttgctt tccatttgta ttccatcttt
    27841 ttcaccagac ttctatttat tgatggacgg attttatgta gaaaaagcag caaacgaaaa
    27901 accgtctgct gctttcagct atctgggaac tatttgcctg gttcagacgc aatgattgct
    27961 agttcacctt ggaccgccca ctcagaaacg ccatttggaa taatacagct tgttcctttt
    28021 tctaatggat atgtttttcc gtcaacgaca aggtctcctg ctccttcgat taccgtcatc
    28081 aatgtataag gggcttgttt tttgaaagat gtgattcctt taatatccca ctcatataca
    28141 ttgaaaaact cagtttctaa ataagtgacg atcgatgaat tcccttttcg gacttctttg
    28201 atttgtaatt ctggtgtttt cgcagggaca gttgttacat cgatcgattg ttggatatgc
    28261 agctctcttg tttgtccttg cgcatctttt ctgtcgtagt catacacgcg gtaagtcgtg
    28321 tcagaacttt gctgtgtttc taaaatcatg attcctttac caatagcatg gatcgttccg
    28381 cttggtacat aaaagaaatc acctttttta actggtactt tcttcaaaag gtcatcccag
    28441 cgcccatctt tgatcatctc agctagttct tctcttgtct ttgcatgatg accatagatg
    28501 atttctgcac caggttcagc atcgatgata taccagcatt cagtttttcc taattcacct
    28561 tcatgtttca tcccataggc atcatctgga tgtacttgta cagatagatc atcttctgcg
    28621 tctaagatct tgatcaataa tggaaacact ggttctgaag gatggccaaa caattccttg
    28681 tgttctgacc atagttcatc taatcttttc cctttgaatt cgccattttc tactacactg
    28741 acaccatgag gatgcgcact gatcgcccaa tcttctccga ttttatcgtt agggatatca
    28801 aaaccaaaga ctgaacgtaa acgacttcct ccccaaattt tttcttgaaa aactggtttt
    28861 aaaaacatag gttcctgcat aattcaaact cctctcattt ttcatagcta ttatagcatt
    28921 atttcggtaa taccgctttc tctttcaaaa agttttcaac aatttcatct cgacatatga
    28981 gatactccgc acgatgatcg acaggatgta cgcgatttga caaaaatatc aacgcttctt
    29041 gtgtttttat atcaatcaag aaaaacgtac ctgtataacc agtatgaaaa agaatcgcat
    29101 gaccgtcttc tacatccttt tttaagtccc atcctaaaga acgatagcca ttttttttcg
    29161 gtgtttgatc ttgcagtaaa gaatcaatcg tctcttcttc taaaaaacga ccattacgga
    29221 tttttccctt gttcaaatac atttggacaa aagtaatgct gtctttcaaa tcaataaaga
    29281 gacccgcgtt acctgcatgt tcagccaaaa ctcttgcttt cggatcatga gtttgtccac
    29341 gcaaaacctc acccgaaggc agttgttctg tgggaacgat ttttttggaa ggaaaaggaa
    29401 gaaataagct gttcatcatt cctaatggtt ccaatacttg ttcttgaaag atttccactg
    29461 cagtttcttg gtaaatttcc tctagcatca acccaagaag aatcgtaccg gcatctgtat
    29521 attttacttc ttttcctagt tgctcacctg gttctagctt caaataagcg gcgatcaatt
    29581 cttcctgaga tagcttatct ctgtttggta tccatgtttt gatatcagaa gtatgtgtca
    29641 gtaaatgacg caaagtgatt gtctgatttt caaattcagg aagatattta tgaagcggtt
    29701 gatcgagttc tatctttcca tcttctaata gacgtaaaac taatgaagtg gttgcaacga
    29761 ctttcgtcag tgaggctgca tcaaacaaca gatcttctgt catcagctgc ttttcaggaa
    29821 cgacttgggc aaagccggct gtaaatgttt ctacttgatt ttcttttatg aaggcgactg
    29881 ctacacctgg ataaaatcct tcttccatct ttttcaaaat aagctgtttc gtgtgtggat
    29941 acatcttttt ctcctgcttt tctatcagat tcttacagtc caagcttaaa gcttaatcta
    30001 tgaggaatca acctttttta cacaaaaaaa ctgcgcgaaa aaccgcgcag tttcccccgg
    30061 tatactattt tctctttcta tctctagaat aagcgatagt atgaccgttc ataaaatgac
    30121 gagacatgcg acaatcgtta agaaagaaaa agttaagcgt cgcatcgaat gtcagtttta
    30181 ttcttttctt ttggctcact gacgaaccac cattcgattc ctatagcatc atagattgtg
    30241 agtactgtgt gctttttgtc taagaagaaa tcaactgata gttgctgcaa gtgagcaatc
    30301 agtccttcca tgctttcagt atcgatcatg aatttcagaa aatcaagtcc aatcacttca
    30361 tctgtaggca aatcaattgc gcctccgcta gcttctgtca aaccaacatg gaaatctcct
    30421 tcattcaata catggatgcc ctcttcttca ttatgtacct tcaaaccaag tacatgatta
    30481 agaaattggc gttcttttgt taagttagta acatttaaat ggattttttc aaaataagaa
    30541 cctgttgata gtttactgaa tttttcttca gattttctta ataatgcttc ttgatccatt
    30601 gtttctggaa tttctctttg gtcgttctta ttctcttcat ggtaaatttc caattcattc
    30661 tcttctgggt ctacgagcaa aatccccata cgtccatgat cttctaaagc ttctttgatt
    30721 ggataattca ttttacggat acgacaaagg atatctgcca tttcttctgt gctagggata
    30781 atcaatgaaa aacggttcag ctttttgatc acccccatgt gatcattcgc tctaggactt
    30841 tcttccagca gcaacatttc acttccagct tcttctgttc ctaaaatggc taattcgttt
    30901 tcttctcttt tcaaatcaaa gccaacaacg ttcttgtaaa aatcaatcat tttatctcgg
    30961 tctttcaccc gaatggctac actttttaaa taggtggcag cccccaattg aaaatctttc
    31021 atatttcttg ctcccctcta cgtcaagctt tattttaacg atggattctt cttctttcaa
    31081 gcaataactt tgattttgtt aaaaaaaatg tgagaaaatt cgcattttgc ttgttttttg
    31141 taaattaatg agtaaaaact cttataaaaa gaggttgtga aaaagctgtt ctgcatcaag
    31201 taataagaac agccaaacaa aaatagctcc tcctattttt cgtttggttg ggcttattac
    31261 cgcagatgca agcttttgaa caccgtttaa ggaagaggtt gtgaaaaagt cgcttagttt
    31321 cgagtagtaa gaaaaatttt gtgaaaatgg ctttctacat ttttgcataa atttggctta
    31381 ataccgagaa actggctttt gaacaccgtt taccaagagg ttgtgagcct gccgtttaac
    31441 tccgagtcac aaggagcaat ttcaaaaaac agcttctcag gtttgttgac tattgtgact
    31501 tgtgaccgag gagttggcag gagaacaccg tttaaggaag aggttgtaaa atcagcggtt
    31561 tgacctgagt attagagata caaaacggaa aaatagcttc tcatattttg ccgttttttg
    31621 agctaatacc gaaggacagc tgattgaaca ccgtttattc ggaaaaaggg actgtgacac
    31681 gacttttgtc acagccccta aaaaaaataa taaatttgta ctattctatg aagcttcttc
    31741 ttcagaaaac tgactgttat aaagatctgc atagaatccg tctttttcca tcaatgtatc
    31801 gtggtttcct gtttctacga tcgaaccatg attcatcaca ataatgttgt ctgcatcgcg
    31861 tattgtagac aagcggtgag caacaacgaa acttgtccgg ttttccaata ggcggttcat
    31921 cgctttttga atcagtattt ctgttcgtgt atctacacta gatgttgctt catcaaggat
    31981 caggacatct ggatttgcta agaatgcccg cgcaattgtg attagctgac gctgcccttg
    32041 cgaaatattg cttgcatcct cgtttaatac agtgttatat ccttctggca atttacgaac
    32101 aaaatcgtct acgtgagcag ctttagcagc ctcaatgatt tgttcgtctg ttgcatcgtc
    32161 gttcccatat ttgatattgt catagatact tcctgtaaac agccaagtat cttgcagtac
    32221 cattgaaaac tgtgcgcgca attcatcacg tgaaagatca cgggtatcta caccatcgta
    32281 acgaatacta cctccagaaa catcgtagaa ccgttcaaga aggttgatca gtgtcgtctt
    32341 ccctgcacca gtcggaccta caatagcgac catctcacca ggtttgacat ccaagttaaa
    32401 atcagtcatt agcaagttat ctggtgtgta gccgaattga acattttcaa aacgaacttt
    32461 gtatggtgta tctttttctt ctggaaggcc agatttttca ttggacatct cttcttcatc
    32521 caacacttca aatacccgtt cggctgaagc aactgttgat tggatagtgt tcatcaaact
    32581 tgcaagttga gtgattggtt gagagaactg tgttgtatat tgcaagaacg cttgaacatc
    32641 ccctaatgtc atgttcccat ttgcaacttt tactccgcct agtacagcga cgaacacata
    32701 gcctagattt ttaacgaaat tcatcaacgg catgatgatt gccgaaataa attgagcttt
    32761 ccaaccagcg gcatacagat tctgattttc tttttcaaat acttcctggt ctttttcagt
    32821 atgattgaat gttttaacaa tggtatgacc accatatgtt tcttctactt ggttattcaa
    32881 taaacctaaa cttttttgct gatctgcgaa aaacacttgt gatttcggcg ctacgatcat
    32941 cacgatgatc aagcttaaag ggatcatcaa taacgcgact aacgtcaatt gccagctgat
    33001 tgaaagcatc atcacgatga ccccaatcaa tgtgactgca ctagttacaa attgagtcaa
    33061 attttgctgc agcgtgcttg cgatgttatc catatcattg attgctcgag acatgatatc
    33121 cccatttgta tgtgtatcat aataggaaat cggtacacga ttcattttgg cttccaaatc
    33181 atgacgtaat tcataaactg ttcgttggga aacacgtgtc ataataaatt gctgtaaaaa
    33241 gttgaataca gcagaaatca aatacatcac gattactgtc gcaatgatct gcgcgatttt
    33301 atcaaagtct atcggaaatt ttgttacttg ttggccttgt tgcatctgtt gagcgccagc
    33361 cattacacct ttgaatattt ctgtggttgc tttccccaag acctttggtg tttggatttg
    33421 gaagatcacg gctgctattg ctagtacaaa tacagcgatg atcgcaacca tacgtttaga
    33481 catatagcca aacaatcggc gtaccgttcc ccagaaattt ttaggctttt caccttttac
    33541 accgagattt ctgccaggtc caccatgtcc tggaccgcct gctggtccat gagagggacg
    33601 tgtgtttgtt ttactcatgc taagtcctcc tctcttagtt gtgaatgaac gatttcttga
    33661 taagtttcgt ttgttgctaa tagttcttca tgtgttcctt tgccaaccag ttttccttca
    33721 tctagcacaa ggatcatatc tgcatccatt actgtactta ctcgttgagc aactaaaacg
    33781 gtgatacttt ctttcatatt cgttttcaat gcttggcgaa gattggcatc cgttttgaaa
    33841 tccaacgctg agaaggaatc atcaaacaca tagatatctg ctgttttgac taatgcccgt
    33901 gcaattgcca gacgctgtct ttgtccacca gagaagtttc ctcctccttg ttccacatgg
    33961 ctatctaagc catcttccat ctctgaaaca aaatctttag cttgtgcgat ttctaatgct
    34021 ttccaaatca tttcatctgt tgcatccggc ttaccatact gcatattgtc tcggatcgtt
    34081 ccagagaaca gaactgcttt ttgaggaaca aagcccatta attctcgcaa attatattga
    34141 gtcatgtcac taatgtccgt tccatttatt tggatcgaac cagattcgat gtcatataat
    34201 cgagggatca aattgactaa ggtcgtttta cctgaaccag tcccccctat gattgcgacg
    34261 atctcgcccg acttggcttg gaaatcaata tcttccaatg caagtttttc tgctccatga
    34321 tatcgataat tgacatgatt gaaggccaat gttgcgtttt ttccagcaaa agaaactgtt
    34381 ttaggatttt cagggtcatg gataccgatt ttttcatcca acacttcatt aatacggtca
    34441 gctgaagctt gtgcacgagg caccatgata aaaatagcgg aaagcatcat aaagctgatc
    34501 aagatctgca tcgcataagt cataaatgca atcagattac cgacttccaa agtcatatct
    34561 gctatgtagt gtccaccaaa ccaagtgatt gctacgtttg taccgctcat gatcaatgtc
    34621 atcattggca tcatcaatgc gacgatcgta ttaactttga ttgccgtatt tgtataatct
    34681 ttattcgcta aatcaaaacg attttcttca aagcgtgtct tatcgaatgc tcgaatcact
    34741 ctgacccctg tcaatccttc tcgaaatacc aggtttaaac ggtcagtttt cttttgcata
    34801 cttttgaata atggaacagc aaagtacatg attccgccga tcaaaataat cattactggg
    34861 atgacaacta agaagatttt tgtcaactga tgatcttttt gataagcaag tatgctcgcg
    34921 ccaatcaacg tgattggtgc attgatcatc atacgtaaaa acatttgtgt aaccatttgg
    34981 atttgagtca catcattggt cgttcttgtg atcaaagagg ccgttccata tttatcaaac
    35041 gcatcttttg tcagactctc tgatttttta tagacatccg tacgcaattg cttacccagt
    35101 ttctgtgatt cacgagtagc aaaaaatgta tttgcgatag cagctaagat actgatcaaa
    35161 gaaaagccga tcatcacaaa acctgtatgc caaatataat cgacatctcc ttgcgctacc
    35221 cctttatcaa taatattgga ggtaagtgtc ggcaaatata aatctgccag tacttggatc
    35281 accatgaaaa ctgcagctgc aattgctgaa gtcaaggaga tccgttttac gatttttagc
    35341 atctagttcc cttctttcta tttatatact ccgtttttca gccaagaaag tttcaaattg
    35401 aattcactga ttgcttgctc tactgagtaa tcttcagatg ttgaaaataa gcgatacgaa
    35461 tgcgcaattg ttgtaaatac agtattcatc actaattgga tcagtaattc caattcatga
    35521 tcatttttta ctttcagctt cgtccgatcg atcaattcaa gcaactcttt tgcatcttgt
    35581 ttgtgttctt tatgtggatc gtgaggcgtt ttttggcgac tcgtatgagg agcgactttt
    35641 cttgaggcac ggtaatccat gttcataaac aagttacgat aaaaagaagc attctcgcct
    35701 tccaaaatct cataaatcat tttagaaaaa taggattcga atcctttgaa tagatcacca
    35761 tcggattctt tcattagttg taacatatct ttagagctgt cacgtctcat tgtctcaaag
    35821 taataaaaat aaagatcctc tttatcttca aaatactgat agaaactgcc tctagggata
    35881 tccgctaatt tgacgatatt agcaatcgaa gcttctttta agggtactct agaaaactcg
    35941 attctcgcag cctcgattag tcttttttgt ttttctttag gcaaatggaa aaatgtctgt
    36001 gtcggcatat ggattctcct ttctttcaag atgacacatt gtcatatgac gctatgtcat
    36061 tataacacct cctacctgtt ttgcaagaca tttgcatagt ttttttcatt atcatccgat
    36121 acattaaaaa agtgataaga ggaatccttc catttgaatt aatgagagtt gcccattaaa
    36181 atagccacat actaaacaaa ggatgagata aatggcccaa tcaattagtg tccaagactt
    36241 caataaattg cctctcgatg ctgacagcat cgttttagat acaagaaata ccttagatta
    36301 cacaaaaaac cacttaagcc actcgctttc tatccccgct gctatcttgc caagacaatt
    36361 aaaaaatcta gatccaaaga aactttatta tattctaagc tatacaggca ggcgatcaga
    36421 agcattagcc gatcaactta ctgctaatgg ttttcgtgct gtttatatca ttggtgggat
    36481 ggaagctctt cagtcgagtg ttgcttgaat attgcttgtt agattcaaaa gaggctgtga
    36541 caaaaaatcg tgtcacagcc tcttttatac cctgtctttt tttagttatt tattcttcaa
    36601 ccttatcaat atgacttgat agccttttct ttttcgagta aactgatttt gttagtcata
    36661 gttataacaa tcatagcaat aaaaggagaa ggtaagcata tggcatctgt tttagtagtc
    36721 aaaggtcatc ccctaactgc agaagaatcg cgcacagtta aggcattgac gtctttttta
    36781 acaagttata aagaaaatca tccagatgat gaagtgactg ttttggaact ttatcgcgat
    36841 gatattccgg aaatcgatga agaattatta tcaggttggg aagcattacg tgctggtgca
    36901 gaattcactt ccttatcaga aagtcaacaa caaaaaattg ctcgtttcaa tgaattgact
    36961 gatcagttcc ttgctgctga taaaatcgtt atcgcaaacg ccttatggaa tctaaatatc
    37021 ccaacaaaat taaaagcttg gttcgatacc gtcaatgtag ctggaaaaac gttccgctat
    37081 acagaaaacg ggccagaagg attagtgaca ggtaaaaaag cactgcacat ccaatctaat
    37141 ggcggtgtat acaacggaca agactttgcc tctcaatatg taaaaggtat tttaaacttt
    37201 gtaggcatcg atcaagtaga tcagctattc atcgaaggaa tcgattatga ccctgaccgt
    37261 gcagatgagt tgatgcaaaa tgcattagat aaagctgctg ctttaggtaa atcattttaa
    37321 aaattgagat aaatgcatac aaaagagaca gaggaaaaaa tcttcaaagc agcatttgtg
    37381 ccttggatat tttcccatct gtctcttttt gttattcttt ctgctttttc aacggatttt
    37441 cttcataaag agaagagatt gttgtgtacc attcaaaaac atgagtaatc actactttag
    37501 ctgctgcata aacaggaatc cccaagatca caccgatgat tccgaacaat ttcccagaag
    37561 tcagcagaac taataaaatc gttactggat ggattgctaa ctgattccct agtactaatg
    37621 gagagatcaa gcgtccttct attgtctgct ctactgcaaa aacgatcagt actttgatga
    37681 tcatgaatgg acctgctaca atggctaaaa agatagccgg aatcatcgct aaaaatgatc
    37741 ctaagtaagg gatcaggttt aaaaaacccg caataatccc caacgtgaca gcatactcta
    37801 aaccaatgac cgcaaaacca atcataaaca tgattgccac agcaaaagcc acagttagtt
    37861 gtcctcttat ataagaagaa acttgactat tcatttcact cagcacattc aacgtcggtt
    37921 tttgcatgcg tgtcggtaaa aatttgacaa aataaggtgc taattgttta ccgtctttca
    37981 atagataaaa taagataaac ggcatcgtca gcagagccac taaaatagtc gctaccgcgc
    38041 cgacaaaact tccaagactc tgaactgtac tcttagatat atcttggatc atatcgcctg
    38101 ccgaattcat gaacttatca cctgctgttt ccaactgttc gcgaaattgg ttgaacagcg
    38161 ggtcccgcaa aatctcggtt aatttattat caatcgtatc tacatattga gggaagttac
    38221 tgatgaagct cactgtttgt tcctgaatct tagggatgat cactacgctc ccccatgcga
    38281 ttagtgcaac aaccaaaaca aacagcccaa tgatactgta aagccttggt acttttcgct
    38341 tttcaaagta atcaactacc ggattcatca agtaatacaa gattcctgct aaaatgatag
    38401 gcagacctac gattgctaaa aattgccaga ttggtgcaaa caaatatgaa acttttgtaa
    38461 ataaaaagac gatcagcaat attaacaata caacaagtaa ggctgtgact acttgattat
    38521 ttaaaaacca gcgccaaaac cacgataccg gtctgttctt cttatttttt ccttccataa
    38581 ttcctcctgt tagttataaa aaatttggct tcccaaacca atagcaggca gtctatgcgg
    38641 atgaagatag attccatttg caatttgtcc ttcttctgga caagcatcaa aaatcaatga
    38701 tacgtgagct actgtcttta aattcccatt ggccatcgga ccgacataat cgatcgtata
    38761 tacctgatca tcaaaagcaa ttgttccgcc tgctttcaaa gaaaaaggtt cgtcttttga
    38821 tacttcttga atcaccgaat attttcgtaa ggcagctgtt gctgttttat cgaacaaaat
    38881 cagcatgggt tcttccttgt ttattgcttc tgtcccaatt tctgtaatct ttgcctgaat
    38941 cattgcactc tctcctttaa ttgctttcaa aaacagtata acacattctc attctttagc
    39001 cgtgtggaat aattatgtga tagaataaaa acgaaaacta tttcattaca attaggagga
    39061 atcagctcat atgatcgaaa agataatttt aggaacctat acgcgtcgcg aaagtaaagg
    39121 aatctatacg atcgaccttg atacagaaaa agaagaatta gccaaccttt cattagctgt
    39181 tgaagaaaat agccctacct atgtagcaaa aagtaaagct ggcaatcttt acacagttac
    39241 tagtgtggat ggattaggtg gtgccggtgc gtacgataag gattttcatt ttttgaatgc
    39301 ggtaacagag tctggcgctc ctttgtgtta tgtagcggtt gatgaagcac gtcaattact
    39361 atacggggcg aactatcata aaggtgaagt aaatgtctac catatattag gagatggcga
    39421 actgaaagca gcagattcaa tattccatca agaagcgaca ggtcctcata aaaaccaaga
    39481 tcatgcgcat gtccactata cggatctgac tcctgaccaa cgattagtcg tatgtgatct
    39541 tggtacagat cgtgtgtata cttatgatgt gtccgaaaac ggcaaattga atcttgtaac
    39601 taccttcact gcagagccag gaacaggccc tcgccatctt gtctttcatc caaatggaca
    39661 attcgcttat ttatttggtg aattagacag cacagtccgc gtgctttctt ataataaaga
    39721 aacaggtgct tttgaagaat tacagaaaat atcgaccttg ccagaagaat tcgacggtga
    39781 aaatggaggg gcagctatcc gcatttcaaa tgatggcaag ttcctttatg cttctaaccg
    39841 aggacataac tcgattgctg tattcgcagt ttttgaagat ggttcccaca ttcaaaacaa
    39901 gcaaatcatc tcaacagaag gggattttcc ccgcgatttc gcactagatc caagcaataa
    39961 ttttgtcgtg tgtgccaatc agaacaccga taatctgaca ttatatcgcc gtcatcctga
    40021 aacaggatta ctggaattac tgaaaaaaga tgtctatgta ccagaatgtg tctgtgttta
    40081 ttttgatcgt tagtaagaaa aggagaatga aatgaatcag caagacgtat tagacagact
    40141 acgggaagaa ctaaatattc ctttctttga tgggaaaatc gaagataaag agtatagcga
    40201 agaggaatat cagaaactca aagccgattt acagaactac tttgaacaat atgtacagaa
    40261 tgtagaaaac taatcagctt tccatactat ctgaaaagaa catcaggaca agacttttcc
    40321 gtctatcctg atgttctttt ctacaaataa aaaaccgccg agatggttag actcgacgga
    40381 aaaggagtat ttactcgatt ttgagtaagt acaaggttgt tgttggttat aggtatgtca
    40441 ttatcataaa ggattttgat cactctatca taaaggtaat attacattta tattagataa
    40501 ctctagtttt tattgttatc aaagatttct tttatttttt caacaacaaa ttagtcattt
    40561 tttggatggc agctgtcaat tggtctgtcg cttggtcttg ttttgcttca gatgtattga
    40621 atagatcttc atctacccct gattcaagat cgatactttg gatcatttct tcacagcctg
    40681 ctacatatga aagataagct ttttcaaaat ttttatgaat gcccattgct tttgctggtg
    40741 gacgcaaagt actgactttc ataagcattg cccgatattt ttttgttcct tcttcaaata
    40801 acaggatgat ctcttcaaga gtcgatttgt ccagttcact tgtttttcct tcatcaattg
    40861 cgtttctgat cacttcatat ttcggatgca ttccttctcc gattgcttct gtttccttga
    40921 cgatttgatc gatggactgc acataatagc caatattcgg taacatgttc gttttcctac
    40981 tttctccatt gttgttatta tttattctac ttgtttttat cctcaaatga caagtacttg
    41041 aaaaaattta atggtttcta tacagctcgc cattcttatt cgtgctcaaa gaaaccatct
    41101 gctaaactat attgtttccc tgcgacaatg tcatattgga tcaattgata ttcgtcgtaa
    41161 atctcttgtt gcgagcgatt gaattgcgct tctttatacc actgtccgtt ttgatagtac
    41221 agttctcgtt caaacgccgg aagttcctgt tctaatgata aaagcagctg ataaaagcca
    41281 tttgttgtct gattcgtctg ttccattaga ttcgctgcaa aataaaacgg actggtgatt
    41341 gcatcgtgag tcgtttgctt ttctaattct cctttactgt cgaacataag aaattgtgtt
    41401 tcatgcaatg tttgtttgtc attttttgct tggatcgtat ctgaatagat ccctggaaga
    41461 tgatcccccc aaaaaacaac taaagtcctt ctagaaagat tcttcaattc ttccgtgaac
    41521 tgcttcagtg ccgtgctgct ataggaaata tcttgcagat aattctctaa gtcaagtgtc
    41581 ccacttccat ctgaagtttt tgcaggatag tctagattat catactttcc atcgtagggc
    41641 atatgtgtct gcatcgtgac taaatgaatg aactgaggtg ttttttcttc cttgagaagt
    41701 gtcattactt ctttgtaagc tgactcatca gaaatatatg gattattctc tatcgtatcc
    41761 gtataggtca tcgtacgctc gctgatgaac tggtcaaatc ctaatgtctg ataaacatcc
    41821 tctcttttat acatcgaagt attgtatgga tggatcgctg tcgtctggta tgattgtgcg
    41881 tttaacgctg aaacgataga aggaagctga tccatttttg gtacaagcat cgtgtaaggg
    41941 gtggtgagtt gcgcattaaa taacgccata gaaaaaccag tcaatgcttc aaattcgata
    42001 tttgctgttc caccgccata gttttgagaa agcatattgc cactatatgt ttgatcagct
    42061 acttcataat aatctgccaa tggctcacca gatacttcta cgccgtttaa tcgactagga
    42121 tctgagaaac tttcactcat cacgaacacg atgttcggcg actcttcctc tacagtttta
    42181 tttttttcat cggctagctt ctggtatttt tcagtgattt ctttgatctt ttctttcgaa
    42241 tatccttcag gttcatccat tggttctacc ttcagattgt acaaaaaccc accgataaaa
    42301 ccagtgttat agtagttcat tttctggctg taaggtatcc atagtgccgt tttattatat
    42361 gcttttcgca acaaattatc gggattgttg aaattactga tataccctag cagtccaatt
    42421 gccacaagca gtgtcgttaa tcggatcaat tggaatcgcc ttcctttttt caggcttcga
    42481 aacatgtacc agcaaaacaa tcctatcacg gttcctgcgg cagctaagat cactgtaaac
    42541 agcatagtgc cggtcatatc tttcagcaat ccgatttcag tgatcatctt caaatcgtct
    42601 ggataaatcg gttcttgccg ataactcatt ttcatataat ttgcaaaacc taatacaccg
    42661 atggctacga catacgtaag ggaacctaaa gggacagatc ctgctagtgc aatcagaaaa
    42721 ataagaataa tcagcaagac gagacaggct aagaagaatt tttccgtatg ccaagaaaaa
    42781 gcaaatttca acgcaaggtc tacggataga tcattttgac accactgcaa aaataaatta
    42841 ctaaaaacaa caagaaaaaa gatactgatg attgctgctg ttccttttaa aatctttttc
    42901 tgtttggtcg aaagtctgct tatatatctt ttcaaatgct tgctccttca tttctatcaa
    42961 taaaagaatg ttgcttctct tattttagtt gtacgaaatt cgaaagtaaa gaacggaaag
    43021 aagaattaat gaaatccaaa tattctcttt ctttatggcc tttctggttt ctttaataag
    43081 cggacctttc gtactccaga aattttttct agctgttgtt tcacttgatt gagctgttca
    43141 aaatcagtac tgtctatgtc aatcaatatg tagacatgct cattcttatg gttacgcgat
    43201 atatctgcga tcgctagctc gttctcattc agcagagaaa atatctctgc taatatattg
    43261 gttttctttt gataaaatat agtgaatcga aacggcgctt ggaaaaacat tcgtgcggat
    43321 ggataattga ccgcttctct aaccgtaccg aacaaaagat aatcttttaa ggcttcaact
    43381 gccaaacgtc cgctgtctgc caaagcttcc tgagtcgatc ccctagatga ggcaacataa
    43441 gtatacggtc attttctaag aactcttctt ctggaaaatc agtcaagtat ttagccagat
    43501 gttgtttttc caatgcttgc ataacagcat atttatcaac aatttcactt cttcctacat
    43561 tgataagtac agcatctttt ttcatttttt ctatattcgc ctgatcgatc aatccttttg
    43621 tatcttctgt caacggcagc atgactacga tgtaatccga ggtagaaaga actgtttcta
    43681 aatctgcttg ccgaacataa tccaactggg catcgcggat agaataaccc aagacatcca
    43741 tccctaagct atagcaactt agcgctactt ttgtgcctat cgctccaaga ccaagaagac
    43801 cgatggtttt gccttgcaat tctcgtccca cataagcact tcttttgttt tctgcctgtt
    43861 ctaaaatatt aggacctgtt agtgtttgta ccatacgact tgcttcgatt atcggacggg
    43921 aagataagag caaacagcac aaaacaagct cttttactgc attagcattt acacctggtg
    43981 tattcatgac gatcgtcccg ttttcagaag ctttttcaac gttgatcgtg ttcaccccaa
    44041 caccagctct tgaaattgct agcaaatctg gtgtaagcca ttcatctttc actttcgtac
    44101 tacgaataac tattccttcg gctggcaaat catctattat tttgaaatat tccccttgta
    44161 acttttctat ctcttgttct ggaatagcat caatcaaatg aacgtgatac ataaaacccc
    44221 ctccttacta acaagtatct cgaaatttct gcaattagac aactgtttac catttgactt
    44281 cctagaagca aaaagagcag ttagagcaga gtaggctgag tgataaaaat aaggctatga
    44341 caagttactg ccataagcct tattttcgag agcaaaatat tgagggtgag atatcttgat
    44401 aaatggtgtt ccgttagctg acattgataa taagactaaa tttcaaaaaa gttgaaaagc
    44461 aaatttcgtc aatctcttct tatttcttaa aactaagtgg ctttttcaca acctcttttt
    44521 atctaataac cactgtttcc aactcaacta attttgccca cgtttcaatt tgatctgttg
    44581 ttagatttag cgagacaacg gtatgatgac cgccacctgc ctgaatccat gaatggacac
    44641 cttcatggaa attcggttta gttttccata acacacgtgc aacaggtaaa ttaggtgctt
    44701 cagtcgttgg ttcaaaggct tctacttcat tgatcagcag tttataatgc gtaccgaaat
    44761 ctgccatcga tacgactacc ccttctcctg ctttcccatc aaatacaaga cgcgccggat
    44821 cttctctatc tcccattgat aaaggagaaa caacgattct tggtttcgtc gcagcaagcg
    44881 tcggatctac ttccatcata tgggattcca agattgcttc ttggccacta gctagctcgt
    44941 aagtataatc ttccatgaat cctgtatctt tccctcgagc catgattttc agcaaccgat
    45001 caatcgctgc agttttccag tcgccttccc cagcaaaacc gtatccttct gccatcaaac
    45061 gttgtacagc tagtccaggt agttgtttca ttccatacag gtcttcaaaa ttagtcgtaa
    45121 aagcgttata gcctccttct tctaagaaac ggcgaatacc gatttcctgc tgagcttgta
    45181 cttttacatg ttcctcccat gtcttttctt cataatcacc atagtcgaag gtatacaatt
    45241 ccttgtactc ttcaaataat tgatcgactt cttcgtctga tacgcgatcg attacttgta
    45301 caagatctcc gataccaaaa taatcaacag tccaaccaaa ttgaatctgt gcttccactt
    45361 tgtctccttc tgtgacaccc acgtttctca tgttgtcacc aaaacgagcc acttttattc
    45421 caaaactttc attataggct actgcaacat ccatccaatc agcaatctct ttttggatct
    45481 caggtcgttt ccagtagcca acgacgattt tattttgttt atttaatcga gcattgatga
    45541 acccatattc gcggtcacca tgagcggatt gatttaaatt catgaaatcc atatcgatcg
    45601 ttttccaagg aatgctttcg ttatattgcg tagctaaatg aagcaatggt ttttgtagaa
    45661 gctttgtccc gcggatccac atttttgctg gtgagaaagt atgcatccaa gtgatcacac
    45721 ctgctacttc ttctcgataa ttgacctctt tcatgatttt tgtgatcgta tcagcagtaa
    45781 ctgctaattc ttgaaggcgg attggataag gcaattggcc attttcattc agttcatcga
    45841 ccatttcttg tgcatgtttc tttacttctc gcagtgcttc ttccccatat aaatgctgtg
    45901 atcctacaac aaaccaaaat tctttttctc caatattcaa cataattagc cactcctttt
    45961 atttgttttg tccgtaatac gcattttttc cgtgttttcg taaataatgt ttatctaaaa
    46021 tccgttgagg cagctcttct gaaaatgaat tgagctgtct ggtaaataaa ttcatcttgc
    46081 atacttcatc tagtacgact gcgttcatta ccgcactttg cgcatctttc ccccaagtaa
    46141 atggaccgtg tccgtgtaaa agtacccctg gaattgctaa gggatcaatt ttgcgttctt
    46201 tgaatgtttc tatgataact tttcctgttt catattcgta cccgctgtcg atttcctgtt
    46261 gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt atctgcatgc gtcgtcccca
    46321 tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc ccatgtcgag tgagtatgga
    46381 cgattcctcc gatttgagga aatgaacgat ataaaacagc atgagtaggc gtatccgaag
    46441 agggattgag tttaccttca atgatatgat tgtccaagtc cacaacgacc atgtcgtctg
    46501 ctgtcatctc ttcataactg actccgctag gtttgataac gaaatatcgt gtttcaggat
    46561 caaaagcgct cacatttccc catgtatatt tgacgagtcc gtgtttaggc aagtcaagat
    46621 ttgcttggta tacttcttct ttcagttgtt ccagcatttt ctatcactcc tttttcttgc
    46681 aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac ggtaacgtct cataaatatc
    46741 tgatagcctg ctatttcttc tgctgaaggt gtgatggtga ttccttggtc ttgtccaaac
    46801 acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc gatccatcat ataggctgct
    46861 aataacgcga ttccccacgc tccaccttct ccggctgtgt ccatcactgt aactggtgcc
    46921 tccatagcag atgctagaat ccgctgtcca acttctggtg ttttaaaaat ccccccatgc
    46981 cccactaaac gatcaatttg gacattctca gatttcaata tatccattcc gattctcatt
    47041 gcaccaaaag cactggctaa atggatccgc atgaaattcg ctaaatcaaa acggctacct
    47101 ggtgtacgta caaataatgg ccttccttca tttactcctg taatgttttc accggaaaaa
    47161 taaccataag aaagtaatcc cccgcaatcc gtatcgcctt ctagcgcttt gttgaataat
    47221 accgtgaata atttatctct gcttatctcc atacccaaag aattggtaaa ttcttcaaaa
    47281 atactgaccc aagcattgat atccgaagag cagttattcg tatgaaccat gcctactagt
    47341 tctcctgaag gtgtagtgac catgtcgatt tccggatgga cattttctag ttctttttct
    47401 aatacgatca tagcgaaggc ggaagtacca gccgaaatgt tccctgtccc tttggctaca
    47461 ctgttcgtag cgaccatacc ggttcctgca tccccttctg gcggacaaac cgggattcct
    47521 ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg attcgcttaa cttgccagct
    47581 tcttctcctg ctactagaac tttaggcaaa atgtgatcca agtcaatcgc tacgtgcgct
    47641 ttttcgatta actgaccaaa aatatttttc atttcttgat ggtaggtttt cgttgaagga
    47701 tcgataggaa acatgcctga tgcatctcct acgcctagta ctttctcacc agtaagctgc
    47761 caatggacat aacctgcaag cgttgtcaaa aaattaattt ctttcacatg ttcttcttta
    47821 ttcaaaatag attgatacaa atgagcgata ctccatctct gcggaatatt gtattgaaat
    47881 ttttccgtta atattcgttc tgcttcttca gtgattgtat ttcgccaagt acgaaatggt
    47941 actagtaact gttccttgct atcaaatgct agataaccgt gcatcattgc actgaaccca
    48001 atcgacccga ttgtcgtaag attctcgcca tagatttcga atacttctgc tgccattttt
    48061 cggtaactga cgcgcagtcc tttccaaata tcttctaatg aataggtcca gataccattt
    48121 tctagtttgt tttcccactc ataactacca gcggcaatag gagtaaaaga tgaatcaatc
    48181 aatacagctt tgatcctagt tgatccaaac tcaatcccta aagatgtacg accatctatg
    48241 acatctaaca tactttgcgc atttacttcc ttcataatga tttaactccc tccttctcgt
    48301 caaacctgat caaaccgtct ttttagtagc gcttacaatg attattgtat acttttgtac
    48361 gtacatgtca agcagttatt tacaagaata caataaaaaa cacttgtttt gtacgtacat
    48421 aagttataaa atgaaattgc ttacaaaatc tgatcaaccg ccttctcgaa attctagaag
    48481 gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat cgtttattta tttttttggc
    48541 gcatttggcg gcattctatt tggatatgat attggtgtta tgaccggcgc cttacctttt
    48601 ttacagcatg attggggttt ggcaggtaaa gcttctttga taggatggat cacgtcttcg
    48661 gtcatgttag gtgcgatcct tggcggctct ttatctggtc ttctttctga taagcttgga
    48721 cgacgaaaaa tgattcttct ttctgcactt atttttatgg ctggttctgt cctttctgca
    48781 tctgcccctc acaatggttc ttattttctg attgcagcaa ggattcttct aggtttagct
    48841 gttggcgctg catctgccct agttcctgct tatatgtctg aaatggctcc tgctagacta
    48901 aggggaagat tgtcagggat caatcaagtg atgattgctt ctggcatgct cctttcctat
    48961 gttgcagatt atttgctaaa agggttgcct gaaacgatgg cttggcgcgt gatgttaggg
    49021 ctcgctgctg tccccgcgct gattttattt ttcggtgttt tagctctacc agagtcaccg
    49081 cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa gagtattgaa ctatattcgg
    49141 acaccaaaag aagctgaaca ggaattcgaa caaatccagc tgaatgtcaa acaagaaaag
    49201 acaaccggaa cttcttggca tactttgttt ttagaaaaat atcgttcctt agtcattgcc
    49261 ggtattggtg tagcagtatt ccagcaattt caaggagcta atgctatttt ttactatatt
    49321 ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg atgcattgat gtggccgacc
    49381 atccaaggaa tcattttagt tgctggttct ttgctctttt tggttattgc agacaagttc
    49441 aatcggcgaa ctttattgaa aataggcggt agcgtgatgg gactttcttt catcctacca
    49501 gctgttttag gaactgtact ggatgctcat acaaattcgt tgttgattct cttgttttta
    49561 tgtatttatg tcgcatttta ttcttgcaca tgggctccac tgacatgggt aatcgtaggc
    49621 gaaatattcc cactagctgt ccgcgggcga gcatctggcc tagcctcttc ttttaattgg
    49681 attggttctt tcctagttgg cttgcttttt cctgtgatga ctgcttctat gtctcaagaa
    49741 atcgttttcg gcatttttgg gatcatctgc tttttagggg ttttatttat tcaagagatc
    49801 gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac aaagtgcatc taaaaaaact
    49861 tatcctaaac gtatttctat ccatcattct taaagaggtt gtgacagaag tggtcagctt
    49921 caagaaataa gaaggaaccg cttagtttcc agtcacaagg aacaatttca aaaaacagct
    49981 tctcatgttt gatgaatatt gtgacttgtg accagttggt aggagaacac cgtttagtgg
    50041 agaggtggtg aaagaaacgt tcagccttga gcgatgacaa aagctctcac aaacattgcg
    50101 acgagtaacc gtaggagcaa taagaggcgg taaaaacccg tctcttattt ttcgttttct
    50161 ttcactgttt cccgttcaac tagtacaggt tcatagagaa tatttggctg tgcctgtcct
    50221 ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc ccaacttttc ttttggatgc
    50281 gtcaatgttg tcaatttttt attccctaaa tggcttaaag aagaatcgtc tgttcctacg
    50341 atcgataggt catctggaat atgatagcca ttttctgtca acttatctag cattaaacta
    50401 gctatttgat cgttatagca aatgatgcca tctaaagctt gttcttcttt caaccgttgt
    50461 actgtttttt cagcgattgt ttctcgtgtt tccgtggtga aagtaatgat atcttccgtc
    50521 aaaaaacgca cattatactg ttcacacgcg ctgataaaac ctttcatcct gtattttcct
    50581 tgaagatcgt cgatttttgt gatgaacaac agacgctgat gtcctttttt tatcaaatat
    50641 tctgctgcca taaaaccgac tttcacatca tctaaacaaa tataagaggc atttaactct
    50701 tcataagcag cattgatcat caccatcgga atgccttgtt ctctcaaagc aacataaagt
    50761 gctaagtttg ggttatactg attacttttc gtaggctcaa cgatcaaacc atctacccca
    50821 taagcaatca ttttcttcaa acactctttc tccaattcgt gatcgttatt cgtactggct
    50881 aaaagcaatg aatacccaga ttctcgtaat tcattttcga ttcctcggat aatcgacgga
    50941 aaaatataat cagagagata tgtggtgatg acaccgatcg tttttgttct gttcaatggt
    51001 gttttttctt tcttatagct gtcatctaca tatgtgcccg accctttttc tttacgcaga
    51061 tagccctcat ttaccaacac agcgattgct tgccgaatcg tatgtctgct gacgtcatat
    51121 atttcttgca gcttgaattc tgagggaatg gttgtattta cttcgtattc ctcacgaagg
    51181 atcttttgtt ttatctcttc tgcaatatct tgatattttg ccatttgcct tgcccctttt
    51241 tatttgtacg tacatatatc ataacgagtc tgcaaggttt attcaagcaa aaatcaaggt
    51301 ttcttgtgct cataaggcat acggaacagg ttcaccaaat tctggaaatc cttgctcatt
    51361 ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg tctaacggat cccctttttg
    51421 gttcttctct ggtcgagcat gatagatcaa aatatcaaag cacccgtctt ctgaaaccgt
    51481 aaacgagttg tgaccaggac caaattgctt atttttttca gaagaaacga atactggctc
    51541 cgataattta tgccatgatt cttttaataa aagattcttg tgctcatcag cccacagcag
    51601 gcccatggca tagttttcat ctgttgcact accagaatac gtaatgaata ttttcccatt
    51661 gcgagtaagg actgctggac cttcatttac tgaaaaaccc cgtttttccc attcatactc
    51721 aggtatagaa aggaggatct gcttcccctt taacgtccaa ggattatcca tttcggatag
    51781 ataaagatta gaattcccag taatttcagg atcttgttgg gcccacacat aatatagctt
    51841 tccttgagaa gagaaagttg ttccgtctaa actgaaagtt tcccttttcg tttttatttg
    51901 tcccttttct atccaatctg tattcattgg atctatatgc tgattttcaa gtacgaacat
    51961 gcggtgatga tgagattcat cacgtatagt tttatgattg cttgcagcaa agtaaatata
    52021 ccattttcca ttgatataat ggacttcagg tgcccagatc aattcactca tcggtccttc
    52081 ttcatgagcc tgccatacaa tcaggcttgc agcatgttct aattcattca gtgactttgc
    52141 ccttcttaat tcaatcactt ggtagcctgg aaccgatcct gtaaaataat aataaccatc
    52201 tgtatgttta tatacccaag gatctgcccg ttgccgaacg ataggattat tataaagtgt
    52261 cttcatcata acaaatttcc tcacattttc tttattctaa ttacattcca agagtatttt
    52321 tctatcccta catttaattc gtcatgtttt tctattgaaa aatagtttat ttcaggtttt
    52381 attttttctg gttcttcacg tgtatttttg tccaacagat cgctactggt taatgtgtag
    52441 tgctctcctt gatctagcaa attgaaatca tgttcaatcg ttatcttttg ttgttcattt
    52501 gtgcgattga tcaagaaaat agtcagctga ttgccgttat ctacgatcac actgtccaca
    52561 taaggaacat tttttatctt ttcacaatca taaacagggc aaatgatttg ttgtttcaat
    52621 acttgacctt tagcatattt cgataagtgt tgcatcacat aataagttgg attcctccat
    52681 gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt ttactaacaa tgattgacaa
    52741 ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc ctaatgaacc aaataataat
    52801 gtatcttcca tagtaaagta acaccaatcg atgggactac cgacttcaaa atccctatgt
    52861 cttttttccg gttgggcatg tacattccat tcatcaaacg ctagatacat tgttttctca
    52921 cttctcaaat atccttttac ataatcacaa gtagcaacga tttcttcaat ttgacgatcg
    52981 aatcgataag cttttgcaag gtatgtcgct gtatcatcac gctcattttt aggaggttta
    53041 gtcaagtctt cttcatcata cttatcaata tagttatgca aagctaaata atctacattc
    53101 tcataagctt cttctaatgc gatacgatcc cattcagggt acgatggtaa acgtggcgta
    53161 gagcttccaa ctaagattgt ttcaattgaa tcatccacta acttcatgac ttttgaggct
    53221 tcatttgcta acctaccata ttcataagca ctcttagccc ccacttgcca tggaccgtct
    53281 agctcattcc ctaaacacca taatttaacg ccaaaaggct ttttttgccc attttttatt
    53341 cttaaatcac tccagaaggt tccttttgga aaattacaat attctactaa attttttgct
    53401 tcatcaattc cttttgttcc taaattcacc gtgaatatcg gtttcgcttg tacatgactg
    53461 atccatttaa aaaattcgtg gagtccaaaa gaatttggtt ctatacctcg ccatgctaaa
    53521 tcaatttttt ttggtcgttg ttctttccta cctacagtat ccacccaatc ataaccagat
    53581 gtaaagtttc ctccaggata gcgaatgaca cctaattcta attcctttac ataagagagg
    53641 acatcttgtc taaacccgtc accatcagct gtttcatggc ccggttcata aatccctgta
    53701 tagacgacac ttcccatatg ctcaacaaat gagccaaaca gccgagaact gatctctcct
    53761 accgtaaaat cctctctgat ttttactttc acttcttcgt ccaacagctt tccctacctt
    53821 tcatttccta tagtcaagat aatagataca agtttccata cgccaccagc aaagataaaa
    53881 ataaaaggag ggaaaagaaa tatgagcagt acaaatccta ataacaatac gcaacaaaga
    53941 atatttctca aaaaatcaaa aaatactttc catgcacttt gttcaaaaat ttgtaaaaat
    54001 gtgatatctt tgttttcaga aatcacatat atccaggata aaagaaacat ggaataaata
    54061 agtaaaacta catatccact aattgaaagt acgattgtct gttcttctaa taattgatta
    54121 acaagaaaat aaataatgat cgttaacaga ctaaggaaca agttcttacc aaaatgttgc
    54181 tttattcctt gaaagaaaaa ataaatgata tttcctgatc cctcaccttt ccattgcgcc
    54241 attacataaa aaacagcaca tgtagcaggc acgatagtaa taacaggcaa acacacgatt
    54301 acccagagaa tacctaataa gatgtaatca gaaaccacaa tcaactgtcc ataaagttta
    54361 gtctcaacta ttttcatctt cctcactctt tcattccaga catagctgtt ccttgaacaa
    54421 aatatttctg tgcaaaaata taaaccacta gtaatggcag cattgaaata accgttccag
    54481 ccattacagg tccataaaaa gaaatgttct gccctgtaaa taaagctaac cctgctggta
    54541 aagttctcat ttttgaacta cttgtcaata tcaatggata aagcaagtca ttccaactat
    54601 tcatcagcgt gaaaatccct aatgataaaa gagtaggttt gctcaatggc aacatgattt
    54661 tcaaaaaaat attaaactca cttaatccat caatgcgtgc tgcctcttcc aaagaatctg
    54721 gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa tgcagttgtc atacgaggaa
    54781 tgatcaaacc tgcataagta tcaagcatgc caagcaaatt gacttcaata aatagaggaa
    54841 tcatgaagac ttggaatggg atcatcattg tcagcaaaac gaaatagaac aaaactgatt
    54901 ttcctttgaa acgcatacga gcaaatgcat accccgcaag agaatcaaag aatacagaag
    54961 tgatcactgt ccctccagca aaaatcactg tatttttgat gtaatctaat aaagggatcg
    55021 ttgtccaaac cttaatatag ttatctaggg tatagctatc tgcaaatatt ctaggcggaa
    55081 atgagataat gtctttttcg tgtttaaaag aagaaataat cagccacaaa aaaggaaacg
    55141 caataaccaa caatataact agcatactaa tcgttcgaat gattttttta ccactaactg
    55201 cttttcgact attcacagta actgtaatcg agctttctgg tgccatacag gtttccctcc
    55261 tatttcattt gtttttcttt ccgtgacatg tacacattag tggagatagc tatagttgca
    55321 acaatgataa acaaaaccga agacagtgct gatgcatagc ccaattcata aggtgctgta
    55381 aatcctcgac tgtaaatata ttgaacagct gtttctgtct taaattgtgg gccaccagcg
    55441 gttgcaacat atacttggtc aaacacttgc atcgaaccaa tcagattcgt taaaatacaa
    55501 aaacctaagg agggaacgat ctgaggtagc gtaatgttaa aaaactgctg tcgatttgtt
    55561 gcaccatcca tttgagcaga ttcatacaaa gagggagaga ttccctgaat agcagtcaag
    55621 agaatcgtca tagtaacacc aaaattcttc caaacagtca tgatggctac cgtcggcata
    55681 gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc cgacttgtga aaacaagtaa
    55741 ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa taatcgcaat tgacgtcaat
    55801 gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac gattgaatct ggtattttta
    55861 gataatgcta ctgctgctaa taaaccaacg ataatttgtg ttggcgtttc gagtacggtg
    55921 aaatataaag aatgaatcaa cgaattaact gctcgttcat cactaaatat acgtgtgaaa
    55981 ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat tcaaatctgt aaaactaata
    56041 gcaaatgttc ctaaaagcgg tatgacagta aataataata aaactaaaaa agatggagtt
    56101 aaaaacaaat acgcagcacg atcaggacga ttccaatatt tggtgaatcc gttcatatag
    56161 tccacctcat ctttcatttt gataaaatat acaatcttgg tttaaacgaa tctgaataag
    56221 atatccgaac aatcgctgtg atttttgcaa taacacctat ctgcaaatac taggtcgatc
    56281 attcagatat ctgtatcttg ccaacagtct atcctgtata aagttctgta ctatttttgc
    56341 agtaattgat tgatggcatc atctgcatcg ttcatgagtg tctcaggatc ttctcctgct
    56401 aataattttt caatcagtgg attgatgata tcgttattga tcgcagagat ttgggtaaaa
    56461 cctggtaaat atggctctgc atagtccatt tgtttcgata attcagatac aataggatca
    56521 ttttttactt cttcatcttt tgctactgaa tccaagtagg caggaaaacc attttcgata
    56581 ctccattttt taccaatttc agttgtattc caatatttca aaaactcata gacagcttct
    56641 tttttattat tatctgtact tgccggaatg gcatatccta cgccatctaa aatagctgtt
    56701 tttttgtctg aatcactccc ttgtggtaag gtagtcacat tataatcaat ctcgtttgct
    56761 ttcaatccgt tatttaacca tggcccattg atttcaatgg ctagttgacc agcattcatt
    56821 aaattgtcca tttcggcgcc agaaatcgat tctggaccag tcttgtctac atggatcatt
    56881 ttttggatcg cttttaacgt tttaaggttc tcaggagacg caaattcaga cttagtataa
    56941 tcatcgttca ctaatttacc gccatttgct aacatccagt tatataacgg tgcagttcca
    57001 tccttattga agacaaaacc tgatacattt ttactactat cagttaattt tggtgccatt
    57061 tctgtcaatt cttcccaagt agtaggtggt tgttcagtat ctaatccagc ttgtttaaac
    57121 aaatcattat tccaatacat atacatgcct tgtacctgca tggggacaaa atactgttta
    57181 ccttctactt cacctaattc aatagccgtt tctttaaaat cagatttatc tactccatca
    57241 tattcccaga aatcgtctaa tggttgtacc gctccgtttt ctacatactg tgcaaaatct
    57301 ggataattta atgcaataaa gtcaggtgct tttttagaag aaatcgctgt aggaagtttt
    57361 tcattcaaat tggcccaagt catgatatcc atttcaattt tgatgttttt atcatttgtt
    57421 ttattaaatt cattaacgat ttcttgcaat atttcaccat cagatgcagt aaacccattc
    57481 caaaaagtca aagtcacttc tttatcagaa tcagattctg ctgaacttcc gccaccgcaa
    57541 gctcctaaaa tacctgttgc agctaccaac aaagagccaa ttaaccattt tttacctttc
    57601 attttttgtt cctccttcat ctgataaacg acatctgacc acattccaag atagcggttt
    57661 caataaacaa ttgattttcc catcttcagc ttttacattt gttgaatgac ttggttttac
    57721 ttgttcactt ttaggaagat tagttgcttt tgtatcaaat ccactcattt cagaaaaatc
    57781 aatcactccc tctaatgata attcagacaa gtgaacagtt agattcattt cttcttctcc
    57841 acggttaact gcaaatatga caagttcttt gtcatttatt acggctatac tttctaaata
    57901 cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa cgaatttgtg ggcttaacac
    57961 ctctccttgt ccatacacag cagcttgcat aaatggataa tagatcgtct gtctccaagc
    58021 ttctccgttt gtttctgtcc tgattggtgc aataacattt accaattgag caagacaagc
    58081 aatctttact ctatctgcat gtttcaataa agtgattaat agacagccta atagtaatgc
    58141 atcttcaaaa ttatagttat cttctagtaa aggaggagca atctgccaag gcgctgcttg
    58201 ctcatcttgt tccgctgaat gataccaaat attccattca tcaaaggaca ggttgatctg
    58261 cttcttacta tgtttcttcg ccttgactgt atcgcacatc gctgtcactt cagaaatgaa
    58321 ccgatccatg tctaaggaac gtgcaagata atgagaaaga tcattttttt ggttgccata
    58381 atattgatgc agagacaaat aatctatttg atcgtacgct tgatcaagaa ctgttaactc
    58441 ccaatcacca aatgtaggca tttgccgatt cgaacttccg cataaaacca cctcaattga
    58501 atcatctacg agtttcatcg ccttagccgt ttcctctgct aaccgtccat attcttccgc
    58561 tgttttatgt ccaatctgcc aaggaccgtc catttcattc cctaaacacc atgtcttaat
    58621 tccaaaaggg ctctcttgac cattcgaacg ccttaaatca ctccaataag ttcctttagg
    58681 aaaattacaa tattcgacta aattacgagc tgcatctact ccacgagtac ccagattgac
    58741 agccatattg atttcggccc ccacatgctg acaccagtta taaaattcat gaatcccaac
    58801 ttcattcgtt tcaattgtcc gccaagctaa atccaacctt ctaggtctat ttgctatggg
    58861 cccaatactg tcttcccaac gaaatcctga cacaaaattt ccaccaggat atctaatcat
    58921 gggaatgttc aattctttta ccaagtgaat gacgtcttgg cgaaatccac ttttattagc
    58981 tgatggatga tctggctgat aaatcccttc atatactgct cgtcccatat gttcaataaa
    59041 tgaactgtac aaccgcggat caactttgcc tacacgcttg acactactaa tcaacattgt
    59101 ggcttccata actgacctcc tttaatttat acatgaataa tacacctcgt tgaaagcgtt
    59161 gtcaataata taaatgttaa acttacttat tttttatcga ttttaattaa ccaaaatatt
    59221 aataatgatt aaaacaaata atttaatata acttttatta acaaatatgt taatatataa
    59281 gaaaaacctt ttaagaggtg atatatatgc aactacaaat aaacgaagaa tccttaccgg
    59341 tatatgaagc attagctagt aaaacaagga tcaagatcat ccagctactt tccaaaaaaa
    59401 agatgaatgt caaagatttg gcaaaagaac ttggagtgag cagtgctatc acgacgatgc
    59461 acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga aaaagtagga caacaaaaaa
    59521 tttcgagcct tcgtgtcgat aaaatcgata tcagttttcc agaaaaaata ttcaatgctt
    59581 ttgatacgaa agaaacatcg atacctattg gtcactatac aaattatgca attgaaccaa
    59641 catgcggttt agctacgatc catgatttta tcgggaaagt ggatgaacca cgctatttta
    59701 tggatcctcg aaggatggat gcccgcattc tctggtttac cagcggattt gtcgagtatc
    59761 aggcacctaa ttttttaaat acagaggata cattggagat gttggaagtt tctgtggaaa
    59821 tttcttctga atttcctttt tcaaatgaca attggccttc agatatcact ttcagcctaa
    59881 atggtgtgga gctaggaaca tggacgagcc ctggagattt tgctgatatc cgcggtaaat
    59941 atacgcctga ttggtatcca gataatctta atcaatacgg cttgctgaag acgatccgaa
    60001 ttaccaaaca cttgacgaat atgaatggag aacccttgtc aaacatcacg atcaacgaca
    60061 tccctaaaga gcaagacact tggcatttgc gaatcgaagt gaaagacgat gccaaacatg
    60121 tggggggctg tactttattt ggtaaaggat tcggtaatta cgatcaggat atcaaattaa
    60181 aagtttacta cagctgatat ttttcttttc tattttataa aacaaaaaca gccaatccct
    60241 atgaagggac ggctgttttt gctttattgt tatttccaga aatcatccaa aacggaaatt
    60301 ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt cgattttttc ttgtgcttga
    60361 gcgggaactt cttttccttc tagataatca tcaatttcat tatatgtgac acctagtgcg
    60421 acttcatctg caatcattgg tttcccatct tcaagatctg cagtcggaat ttttgtgtat
    60481 aatttttccg gtgcattcaa tgcttgaagc aattgtttcc cttgtcgttt gtccaaacgg
    60541 aataatggca aaatgtcggc tccaccatca ccaaatttcg tgaagaatcc agtaatgttt
    60601 tctgcagcat gatctgtccc taatacggct cctgctcttt ctccagctac ggcatattgc
    60661 gtaatcatac gttgacgtgc tttgatattt cctttgttaa aatcgctgat tcccacacct
    60721 gcttcgctta atacttgtac ttgtgcatca acagctggtt tgatatttac tcgcagacta
    60781 acatcgggct ggataaagtc aagagcagct tttgcatctt cttcatctgc ttgttcacca
    60841 taaggaagac gaacagcgat aaattgatag ttttcatctt ttgtttcttc acgcatttct
    60901 tccatcgtta gttgcgccaa acggcctgct aaagtagaat cttggccgcc gctgatacct
    60961 agtacaaaag ttttaagaaa cggatatttt ttcaaatatg ctttcataaa gtcaatactt
    61021 ttacgtattt cttcttttgg atcaattgtt ggtttgacac ctaattcatt gataatttct
    61081 gtctgcaggc tcatttattc tcctcctatg cttctgtatg aagttctgct acgctttttt
    61141 tcattcgttc catgatagcc attttatggt tccagcattc agtagacaga tcgactgggt
    61201 atttttgcgg attcaagtct cgtttatact cttcccatag tgaatctaga ttttcttttg
    61261 tatattcttt gatttcgttt agtgcaggta attcgtaaac tctttttcct tcgataaaga
    61321 tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt tttattgata aacgtatgca
    61381 ctggatggaa catgaagata gcttcttctt ctcgagggtc ttcatcccat aatgtcacgt
    61441 agtctccttc agatttccca tcaaaatttc gagtgatgcg ccacacttgt tttttccctg
    61501 gagtagttac tttttctgca ttgcttgata acttgatcgt atccatcatt tttccttcat
    61561 catcttcgat cgatactagc ttgaatactg caccaagggc tggctgatca taggctgtga
    61621 tcaattttgt cccaacaccc catacatcaa ttttagcttt ttgcatcttt aaattcaaaa
    61681 tcgttgcttc atccaagtca ttcgaagcat agatttttgc ttccgtaaat ccggctgcat
    61741 ctaattgttc ccgaacacgc ttagatatat aggccatatc tccactgtcg atgcgtacgc
    61801 cttggaagtt gattttgtca cctaattctt tcgccacgcg aatcgcactt ggcacacctg
    61861 atttcaacgt atcataagtg tcgactaaga acacgcagtc tttatgggtt ttagcgtagg
    61921 ccataaatgc ttcataatca tttccataag attggactaa cgaatgagca tgggtcccgc
    61981 tagctggtat cccaaatatc ttcccagcac gtacattgct tgttgcatct gctccgccga
    62041 tatatgccgc tctagttccc cagactgctg catccagttc ttgtgcccgt ctagttccaa
    62101 attccaatag tggatcttct ccgatgacag atttgatacg agctgctttt gtcgcgatca
    62161 acgtttggaa attgaccata ttcaacagtg ctgtttctac taattggcag tgcgctaaag
    62221 gcccttccac ttggattaat ggttcatttg caaaaactag ttctccttca cgagcagaac
    62281 gaactgttgc tttaaattca aaattttgta aatactccaa aaaaccttca ggatagactt
    62341 ctaatccacg caagtaagca atatcggaat cattgaacgt cagattctct aaataattga
    62401 ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt aaaaggcatt tctcgaaagt
    62461 aacattcaaa aacagcatgt aaatcagctc gacctaattc ccagtatgtc tgcatcatat
    62521 tgatttgata tagatccgta tgcagcgtca aactgtcgtc tgggtaagta ttattcatct
    62581 tgtattactc ctcttggcaa gttttcattt cctacccatt ataacaaaaa aaattaatag
    62641 gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt tacggatatt tcttgctaaa
    62701 aatgaactcc atctgctaaa actatccttt cttattggta tttaacctaa acgagaaaga
    62761 atacttattt tattcaatgt atccgagaca cgaagaaatt agcgttttct atttttctta
    62821 cttgataaaa taagtagatt ttcctactac aatcgtagcc gttaagatac cgatgattga
    62881 acaaatataa ccaaatgtct ctacaacata atcaatggca aaagtttttg aagcaataat
    62941 acgtctttcc ttttttgctt cttatcccca aagaaacgga tataacttat tatgtattga
    63001 atttttttgg aaaggcgaat tatgacaaga tataccttgc gttgaaaaaa ctcgcggaaa
    63061 aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc agctgtcaat agaatggaaa
    63121 caaaaatggc gagacatcaa tcagtctcgc cttgtttttt attgtataca ttagatatgg
    63181 agcaggttac cataaagatg attattagtc gttattaaat agaatccttc gtattttttt
    63241 gttctatcac tacaatatct ttatttggac gtttgaattg tattccggca cgttctagct
    63301 cctgtttcac tcctacatgg actttgacat cttctcgtgt catttttaaa acgtgatgaa
    63361 tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt attgatcatc acgacttgct
    63421 ttttatcata tgtgtctacc aatagcagtt gtgttcgatc taacgaaaat aatttgtaat
    63481 aattaatagc cgtattccca aaaaagaatt ccactacttt aggataaaat gtcttaatat
    63541 ttaaattact attcacaggt gtttcgatta gcttcatctc attcctcttt tctatatagt
    63601 cgatttagat ccaatcttaa gttatgttac catctttcct agcattaagg aatcggttac
    63661 aattgaacct ttaaagacaa aaggatttac cttttatcac ttaatagtat aacataattt
    63721 cgtgagaaaa aaatacaatt ttttcttgac catctcgtta cgtaaaggat tatgcttaca
    63781 tttgtcagga ggaaaaataa tggagtacac aataaagaaa atgtcagaga tatcaggagt
    63841 cagtccgaga accttgcgct tttatgatga gattggactg ctaaaaccag ctaggatcaa
    63901 ttcttcaggt tatcggattt atggcaaaaa agaagtcgat cgtctgcaac acatcttatt
    63961 ttatcgaaca atggcattca agttggatga tatccaagag gtcttagaca atccatcttt
    64021 tgaccaccag aaagctttaa tcaagcatca gcaaatgttg ctggaaaaac gggcacaaat
    64081 cgataccctc ttgacaactg tccaacagac acttgatatg tacgaaggag gaagaaaaat
    64141 gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag ttgaaagaaa atgaagaatc
    64201 ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag gttacagcat ccaatgaaaa
    64261 atggatgaat atggataaag aaacgtatga agaaatgcaa actgttgaaa agcagatgtt
    64321 atttgatctc actctttact taaaagaacc agaagatgaa agcttagcag accgtatctt
    64381 ccaagctcat aagaagtggt tctcttacag ctggcctgaa tatcagccaa aagcgcataa
    64441 aggactagga atgatgtacg aagccgatga acggttcact gcatattatg acgagcgaag
    64501 tggtcatggt gcagcaaaag ctttaaatga aattatccaa aaaaaggcgt aatcttttac
    64561 atgatccaac aaaaaagtac acgtaaaatg acctaataac aaaacagcag acacccaagt
    64621 tagattttta tgctaacttt tggtgtctgc tccattcttt cacaagtttt ttcatcctac
    64681 tttaaatata ttcatcgttg aataaacgta taaggtattt tcactttatc ctttttttct
    64741 gtaagaaaca attgaaatgc catttctccg caagcttcca gatggtggtc gatggttggg
    64801 aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa tcaccactgg tgtcttatct
    64861 tgcagatagc tttgcaagat gcctgctgca accatatccg aatttgtaaa gacaccatca
    64921 actccaagct ctttaaaaaa gaagcctgct tgttttcctt tctctgcatc tatacaatcc
    64981 caaaaaacaa gtgattcatc aaaagatgga aaaagttgat gacatagctg caaagtgatt
    65041 tttgagttat aacttagcct gccacttcgc cccaacgtga tgcccaattg ctttatacct
    65101 tgctgcttca aataagtaag accttctgta attgattgtt ctcgatcaat aaagacacag
    65161 cttgcctgtt ttcccttgat ttcttcacaa aaaactagag gtccatattt ttgatatgcc
    65221 aataaatcat ctagtgtatt ggcacgagac gtaatgatca atccatcgaa agctttagct
    65281 gcaaattctt caagatacct tcgctctttt tctttatgat agcccgtcgg taaaagtgtc
    65341 actttgtaat cataatcaaa cgctgcttca atgatcccac tgatcaactg atcaaaatac
    65401 ggatgattgg tataaggcag aatcactcca atgcttttcg tttttccata acttaactgt
    65461 ctggcatttg cattagggat ataatcgact tctttcatga ccgcaagtat ttctttcctt
    65521 ttttcctcat ctacatatgg atgtccattg attacacgtg aaatagtaga aactgaataa
    65581 cctgtgagct ttgcgatatc tcgaatgttt gccactgctg cttcgccacc tatacaaaaa
    65641 gattttcttg taaatagtct accacagctg gaccgcattc aaaaaactga tacatttttc
    65701 cttcatgcga aaaactgatc cgctgcccgc cccaatatat atcttttgtc aaattttcga
    65761 tttcattcat tggtatttct tttactatat tcctattcaa cccttgaaaa ctgtacaacg
    65821 ttaatacgga tcccttgaac tcgacaaacg cataaaaata attgttcgtt gtgccatctg
    65881 ttatctctac ataagtattc attgcaagcc ctccttttga tactttgagt atagtgtatg
    65941 aaacgcgtct catagcaagg agtttgtttt gccaaagaaa agttttattc ctctttcatt
    66001 attaccaaag tctcttattt taaattgaat acattataat gatactgtaa atatatcttt
    66061 attattagtg aat
//
LOCUS       DANKEF010000010        65398 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000010 DANKEF010000000
VERSION     DANKEF010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.010"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            410..2575
                     /gene="acm"
                     /locus_tag="PWZ48_000719"
     CDS             410..2575
                     /gene="acm"
                     /locus_tag="PWZ48_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HDL2260395.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(2847..3332)
                     /locus_tag="PWZ48_000720"
     CDS             complement(2847..3332)
                     /locus_tag="PWZ48_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HDL2260396.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    3422..3767
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            3812..4453
                     /locus_tag="PWZ48_000721"
     CDS             3812..4453
                     /locus_tag="PWZ48_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2260397.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            4472..5107
                     /locus_tag="PWZ48_000722"
     CDS             4472..5107
                     /locus_tag="PWZ48_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2260398.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            5125..5940
                     /locus_tag="PWZ48_000723"
     CDS             5125..5940
                     /locus_tag="PWZ48_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2260399.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            6259..7695
                     /locus_tag="PWZ48_000724"
     CDS             6259..7695
                     /locus_tag="PWZ48_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2260400.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            7884..10331
                     /locus_tag="PWZ48_000725"
     CDS             7884..10331
                     /locus_tag="PWZ48_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2260401.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            10561..11628
                     /gene="rlmN"
                     /locus_tag="PWZ48_000726"
     CDS             10561..11628
                     /gene="rlmN"
                     /locus_tag="PWZ48_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HDL2260402.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(11821..12924)
                     /locus_tag="PWZ48_000727"
     CDS             complement(11821..12924)
                     /locus_tag="PWZ48_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2260403.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            13477..14025
                     /gene="aac(6')"
                     /locus_tag="PWZ48_000728"
     CDS             13477..14025
                     /gene="aac(6')"
                     /locus_tag="PWZ48_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2260404.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
     gene            14571..16232
                     /locus_tag="PWZ48_000729"
     CDS             14571..16232
                     /locus_tag="PWZ48_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2260405.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(16302..16649)
                     /locus_tag="PWZ48_000730"
     CDS             complement(16302..16649)
                     /locus_tag="PWZ48_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HDL2260406.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            16733..17674
                     /locus_tag="PWZ48_000731"
     CDS             16733..17674
                     /locus_tag="PWZ48_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260407.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            17675..18718
                     /locus_tag="PWZ48_000732"
     CDS             17675..18718
                     /locus_tag="PWZ48_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260408.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            18724..19794
                     /locus_tag="PWZ48_000733"
     CDS             18724..19794
                     /locus_tag="PWZ48_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260409.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            19784..20737
                     /locus_tag="PWZ48_000734"
     CDS             19784..20737
                     /locus_tag="PWZ48_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260410.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            20749..21759
                     /locus_tag="PWZ48_000735"
     CDS             20749..21759
                     /locus_tag="PWZ48_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2260411.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(22044..22865)
                     /locus_tag="PWZ48_000736"
     CDS             complement(22044..22865)
                     /locus_tag="PWZ48_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2260412.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(22962..23240)
                     /locus_tag="PWZ48_000737"
     CDS             complement(22962..23240)
                     /locus_tag="PWZ48_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260413.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            23508..25517
                     /gene="metG"
                     /locus_tag="PWZ48_000738"
     CDS             23508..25517
                     /gene="metG"
                     /locus_tag="PWZ48_000738"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDL2260414.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(25996..26583)
                     /locus_tag="PWZ48_000739"
     CDS             complement(25996..26583)
                     /locus_tag="PWZ48_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260415.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            26758..28080
                     /locus_tag="PWZ48_000740"
     CDS             26758..28080
                     /locus_tag="PWZ48_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2260416.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            28235..29011
                     /locus_tag="PWZ48_000741"
     CDS             28235..29011
                     /locus_tag="PWZ48_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2260417.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            29004..29573
                     /gene="rnmV"
                     /locus_tag="PWZ48_000742"
     CDS             29004..29573
                     /gene="rnmV"
                     /locus_tag="PWZ48_000742"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HDL2260418.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            29566..30450
                     /gene="rsmA"
                     /locus_tag="PWZ48_000743"
     CDS             29566..30450
                     /gene="rsmA"
                     /locus_tag="PWZ48_000743"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDL2260419.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            30901..31530
                     /locus_tag="PWZ48_000744"
     CDS             30901..31530
                     /locus_tag="PWZ48_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260420.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(31654..32763)
                     /gene="ugpC"
                     /locus_tag="PWZ48_000745"
     CDS             complement(31654..32763)
                     /gene="ugpC"
                     /locus_tag="PWZ48_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HDL2260421.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            32972..33394
                     /gene="mgsA"
                     /locus_tag="PWZ48_000746"
     CDS             32972..33394
                     /gene="mgsA"
                     /locus_tag="PWZ48_000746"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDL2260422.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            33717..34253
                     /locus_tag="PWZ48_000747"
     CDS             33717..34253
                     /locus_tag="PWZ48_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2260423.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(34293..36056)
                     /locus_tag="PWZ48_000748"
     CDS             complement(34293..36056)
                     /locus_tag="PWZ48_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260424.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(36059..37774)
                     /locus_tag="PWZ48_000749"
     CDS             complement(36059..37774)
                     /locus_tag="PWZ48_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260425.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(38553..38777)
                     /locus_tag="PWZ48_000750"
     CDS             complement(38553..38777)
                     /locus_tag="PWZ48_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260426.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            38968..41745
                     /locus_tag="PWZ48_000751"
     CDS             38968..41745
                     /locus_tag="PWZ48_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2260427.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            41826..42245
                     /locus_tag="PWZ48_000752"
     CDS             41826..42245
                     /locus_tag="PWZ48_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HDL2260428.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            42257..42727
                     /locus_tag="PWZ48_000753"
     CDS             42257..42727
                     /locus_tag="PWZ48_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2260429.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            42744..43523
                     /locus_tag="PWZ48_000754"
     CDS             42744..43523
                     /locus_tag="PWZ48_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2260430.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            43510..44331
                     /locus_tag="PWZ48_000755"
     CDS             43510..44331
                     /locus_tag="PWZ48_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2260431.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            44348..45397
                     /locus_tag="PWZ48_000756"
     CDS             44348..45397
                     /locus_tag="PWZ48_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2260432.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            45410..46414
                     /locus_tag="PWZ48_000757"
     CDS             45410..46414
                     /locus_tag="PWZ48_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2260433.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            47047..48066
                     /locus_tag="PWZ48_000758"
     CDS             47047..48066
                     /locus_tag="PWZ48_000758"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2260434.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
     gene            48109..48990
                     /gene="galU"
                     /locus_tag="PWZ48_000759"
     CDS             48109..48990
                     /gene="galU"
                     /locus_tag="PWZ48_000759"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2260435.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
     gene            49290..49667
                     /locus_tag="PWZ48_000760"
     CDS             49290..49667
                     /locus_tag="PWZ48_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260436.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            50273..50887
                     /locus_tag="PWZ48_000761"
     CDS             50273..50887
                     /locus_tag="PWZ48_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2260437.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            51660..53399
                     /gene="yfcC"
                     /locus_tag="PWZ48_000762"
     CDS             51660..53399
                     /gene="yfcC"
                     /locus_tag="PWZ48_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDL2260438.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            53556..54740
                     /gene="iadA"
                     /locus_tag="PWZ48_000763"
     CDS             53556..54740
                     /gene="iadA"
                     /locus_tag="PWZ48_000763"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDL2260439.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            54851..55054
                     /locus_tag="PWZ48_000764"
     CDS             54851..55054
                     /locus_tag="PWZ48_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2260440.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(55155..55817)
                     /locus_tag="PWZ48_000765"
     CDS             complement(55155..55817)
                     /locus_tag="PWZ48_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260441.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(55830..57332)
                     /locus_tag="PWZ48_000766"
     CDS             complement(55830..57332)
                     /locus_tag="PWZ48_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260442.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            57880..59559
                     /locus_tag="PWZ48_000767"
     CDS             57880..59559
                     /locus_tag="PWZ48_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2260443.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            59686..60165
                     /locus_tag="PWZ48_000768"
     CDS             59686..60165
                     /locus_tag="PWZ48_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HDL2260444.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            60384..61064
                     /locus_tag="PWZ48_000769"
     CDS             60384..61064
                     /locus_tag="PWZ48_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDL2260445.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            61072..62403
                     /locus_tag="PWZ48_000770"
     CDS             61072..62403
                     /locus_tag="PWZ48_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2260446.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            62418..63380
                     /locus_tag="PWZ48_000771"
     CDS             62418..63380
                     /locus_tag="PWZ48_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HDL2260447.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            63491..63829
                     /locus_tag="PWZ48_000772"
     CDS             63491..63829
                     /locus_tag="PWZ48_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HDL2260448.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            63908..64615
                     /locus_tag="PWZ48_000773"
     CDS             63908..64615
                     /locus_tag="PWZ48_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HDL2260449.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(64718..>65398)
                     /locus_tag="PWZ48_000774"
     CDS             complement(64718..>65398)
                     /locus_tag="PWZ48_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HDL2260450.1"
                     /translation="FFYCFFPNQISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQF
                     PLNHLENKIPIYFYFLVLAVVFFELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREK
                     IDTIFSKNGERNQISQYLSQLIQHLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLF
                     RKDAVKITAYSEHADLIITDQHEKISDETETFYLDTTHNTVLFGKLIKAVHELYMKKG
                     YENKTKRM"
BASE COUNT        21556 a        11269 c        13608 g        18965 t
ORIGIN      
        1 tttttttatt ctaaataaag aaacagatgt ttctttatat tgtattttat cctttaatta
       61 acacaaaaac agctagtata aactaataat gttaattaaa agtgtatttt atgaaactat
      121 cgttatttat taatgttgca tttttgtaag ttaaaaattg gaaatataga ttgaaaaaat
      181 gaaagcaata agtaaaataa aatgaatata tgcgaaaaaa taatttacat taggaaaaac
      241 cttgtaaaaa cagttttttg gcatatttat tttttataaa tatttgtaaa ataactatca
      301 attttttagt taaaagattg gaaatgagtg ggggaggaat tgaagaaagg atttttgaga
      361 gatgatatag taggttttat ttttagttaa aataggggag atagaaaaaa tgaaaaagtg
      421 tagaaatatt attttatcaa tgttatttat tattactaat ataacatcac tgattcctgt
      481 tcatgtctat gcggatgcag gcagagatat cagcagtaat gtcacttcgt tgaccgtgga
      541 cccaacgaat attacagatg gaggaaacat aaaagtaaaa ttctcgtttg acgaaaaaaa
      601 acaaaatatc caaccaggtg attatctttg gatcaattgg ccaagtgaag gaaatattcg
      661 cggtgaagga ttccaaaaag aaatcccatt aatgattgaa aataaaaatg ttggaacttt
      721 aacggtcaga aaagattctg cgcaggttgt gtttaatgaa aatattaaaa acctagactc
      781 agtagaaggt tgggggcaat ttgagataca ggccagaaac gtaaccgata ccggtgaaga
      841 aaatacggga ccattcaccg tgacgagcgg tgataaaaca gctactgtaa atgtgacaaa
      901 gccagcttct ggttcttctt ctagcgtttt ttattataaa acaggagaca tgctacctga
      961 agatactaag cacattcgat ggtttttgaa tattaacaat aacggcactt atgttgaaca
     1021 accagttaaa atcagtgatg aaatccaaag tggacaaaga ttagatccaa gtacgtttga
     1081 aattaatcaa atacacttag gtgaacagaa ggtttataga ggagaagagg gaatccaaca
     1141 atttttacaa gatttcccca gcgctacatt taatttttca gtaactgata attacattga
     1201 aataactata ccaaaaaact ttgtcaatct taggaaaata atggtgagtt ataagacgat
     1261 catagaaaac ccagaacaaa taaattttga gaatcattcg gaagcttggt ttaaagaatt
     1321 taataaacct gctgttgatg gagaaagttt caatcataca gttaaaaata tttcagcttc
     1381 tggtggagtc aacggtacag ttagaggcga attgaagatt ttcaaatata tcaatgacac
     1441 agagattggt atcccaaatg tgactttcga attaagacga gcagatgaac agcctattca
     1501 aggacagtct agtatcctgt tgacttcgaa tgaacaggga gaagctagta tcaaaggcct
     1561 gcaagtagga gactatgttg taaaagaaaa agaagcaccc aattggattg attttgatcc
     1621 actaagttca aatgaactca aattctccat aaacgaaaat gatacagagg gagtcagttt
     1681 acctatttat aacaagaaaa aggttacgaa catcacagca actaaaattt ggaacggtgg
     1741 aaccactcca agaccgagta tttattttaa attattcaga gcaagtacaa acaattggga
     1801 accagttcct gatgcagaaa caaaacgatt agacaatgga ataacttcag tgacttggga
     1861 agatatccaa cagtatgatg atagtggaaa cgaatatacg tttaaagtgc aagaagtaga
     1921 tcaaaacgga aatgattatg tcccttcagg ttatcaaaaa attgaaaacg ggctagtcgt
     1981 tacaaatgag aataaagaag tcatttcagt ctcaggacaa aaaacatggg aagacaatga
     2041 gaaccaagac ggcaaacgac cgacgacgat cacagtaaat ctattagccg acggtcaacc
     2101 gatccaacac aaagaagtaa gtgaaaaaga tgattggcgc tatcgtttca caaatctgcc
     2161 aaaatacaga gacgggcaag aaatcatcta tactgtaacg gaagacaacg tgcccgaata
     2221 cagtacgacg attgacgggt ataacatcaa aaacagttat acaccggaaa aaacaaatat
     2281 atctatacca ggtagccata taacaccgtg gaaaaatttt ccagagaaga cagagagaga
     2341 gaaaattaga aatctattct ctcaaaaatt gcaaatattt gctcatacag gagaatccag
     2401 cgaacaaaga agcaattata ttagtaagag agctgtacct aaacagataa caaataaatc
     2461 aaaaagcatt cttcctaaaa caagtagtaa aaaatctagt tttgctgtaa tcattggttt
     2521 attgatagta actatctgtg ctgggatatt cttacaaaaa tataaaaaag tttagttaga
     2581 atataatatt ttttgttaaa tatgactgaa atcaaaagaa taaaattccg atttataatc
     2641 gaaaaaagaa gcatttttat atatgcttct tttttcgtag aatcaataaa tatggatttg
     2701 gctaactatg gaagaatact cgtattagtt ttcagcgttc atactagttg ttacattgtt
     2761 tgattggcca aaagaatttc tgatatattt tgtgctaaat aaagaaaaag acgcttgaat
     2821 tgggaaataa gaaaactact ctatcattac aaattttcat aaactggcag atgttctaag
     2881 taaacaagta tcttttcaaa gtcggaaaaa tccataatca acgtagtggt attgatattt
     2941 ggatgaaagc ctacggtatc ttgacgatca atagcagcat cgatcacgac ttggattttt
     3001 ttctctttgt cgtgcatcaa tgctaatgga gtcactgtac ctgctggaac gcctaggagg
     3061 tcaggaagct gttcttcaga aacaaaagat aagcgctttt cttttaattg ttcagctagt
     3121 tgttttaaat cggcgtgctt ttcatcaggc aagatgacaa gatagatttg ttttcctttt
     3181 cttgatttca ataatagatt ttttacttgc ggaccaggta gggaaaaagg gacatttttg
     3241 actgacgtaa tcgcaggatg atcgactttt tgaaaagaga tacccagttt ttttagtagt
     3301 tcataagctt cttgttcggt tgcacgactc atcgcgcatc accctccttt ttttattagt
     3361 gtatcataaa aaagagttga caaatgaaaa ataatcatgt aaattacacg taaagttaaa
     3421 acaaatgaca tcgaaaagaa gagtagccgt taggtgtttt tagagagtct ctggttggtg
     3481 gaaagaggca gcagttaacc gtgaaacaca tcttggagtc gattttctga agcgtatagt
     3541 aggaaaatcc ggggagcccg ttacagctgc ggtcgttgct agatgaccag ataaggctgg
     3601 cttttccgtg aggagtcggt aaaaaaaggt ggaaccacgt gattaacgtc ctttgatctt
     3661 cggatcaaag gactttttgt tttcatctga acggcaacga ctgtgtgagg ttttttctgc
     3721 aaggaaaaaa taaacagagg tggaaccacg tataatacgt cctcttggca tttgccaaga
     3781 ggattttttt attttaaagg aggaaaagaa gatggagtat ttgattgaag ttatgccgca
     3841 attattagta ggagcactca caacattgaa attattttta tttaccttga ttggctcatt
     3901 accattaggg attgttttag cattaggatt gtctttgcat tttaagccgc tacgctactt
     3961 gcttcaaaca tatgtatggg ttatgcgggg aacaccgctg ttgttgcaac tgatttttat
     4021 tttttatggg ttgccgacga ttgggatcgt ttttgatcgt ttcgaagcag cactcattgc
     4081 ctttatctta aactatgcag cttattttgc agagatcttt cgtggaggga tacaatcgat
     4141 tcctcaaggt caatatgaag cagcgcaagt tttgcaatta agtccctttc aaacggtgac
     4201 gaaaatcgtc atcccacaag tgacaaagat tgttttacct tctgtaggga atgaagttat
     4261 caacttggtc aaagattctt ccttgatcta tatattaggt ttaggtgacg taatgcgtgc
     4321 tgggaaaatc gcgatggaac gcgacgcgac tttactgcct ttgctaggcg tggcaatcat
     4381 ctatcttaca ttgactggta ttctaacaat cgtaatgaaa caagttgaag gaaaattaaa
     4441 ttattaccgc taactgatag gaggaagaaa tatgattgaa ttgaagaaag tgaacaaagc
     4501 atttggtgaa aaaaaaataa tcaaagattt gaatctaatc attccagatg ggaaaacatt
     4561 agctatcgta ggtccttccg gtggcggtaa aaccacgctt ttacgtatct tggctggctt
     4621 agaaagttct gacagcggag aattttattt agacggtgta ccgttcaacc cattcgagct
     4681 taaaatgaaa gaacaagttg taggagtcgt attccaagac ttccagctat ttccacatct
     4741 gaatattttt gaaaacatta ccttagcacc aaaaatggta ttgaaacaga caaaagaaga
     4801 atacaccaaa aaagcagaag aattagcggc ttcattaggg attagtgatt tattgaatat
     4861 ctatccttat cagctttctg gcggacaaaa acagcgggta gcgattgcca gagcattggc
     4921 tatgaatcca cgggtattgg cttatgatga accaacaagt gctttagatc ctgaattacg
     4981 ccagcaagtc gagcgtttga tcctatcatt gaaagaagat ggggtaaccc aaattattgt
     5041 cacccatgat ttagtatttg cagaaaatgt cggtgatcaa ttactaaaag tttccccaat
     5101 tcattaagaa aggagaagat agagatgaaa aaaaccagtt ttctattcgc tttgatcgca
     5161 ggtcttcttc tttttgcagg ctgttcgaac aaaaaaactt cagcagataa ttgggcgact
     5221 atcaacgaag agaaaaaaat cgttataggt ttagacgata catttgttcc tatgggattt
     5281 agagataaag acgggaagct cacaggattt gatattgatt tagccaaagc agtctttgaa
     5341 caatatggga ttacggtaga ctttcagccc attgactggt caatgaagga atttgaactg
     5401 aacaatggaa cgatcgattt gatatggaac ggctattcca aaacgccggc aagagaaaag
     5461 aaagtgcaat ttacaaaacc ttatatggaa aacgatcaag tgctgatcac acctaaagaa
     5521 tcaggaatca caaactttgg acaaatgaat aataagagat taggtgcgca aaatgggtca
     5581 tctggttatg atgtatttac gaaacaacca gaagtattaa aagagatagt caaaaaccaa
     5641 gatgctgttt tgtatgatag ttttaatgaa gcattgatcg atctgaaaag cagacgggtc
     5701 gatggtctgc tgatggataa agtatacgca gactattatt tgaaacaaag aaacgaacta
     5761 gaagacttca acataacaaa aggttcttat gagagtgagg attttgcagt tggtgcacgc
     5821 aagtcagata gtacattagt gaaaaaaatc aatgaagccc tagatcaact agagaaaaca
     5881 ggtgaattcc aaaaaatatc cgataaatgg tttggcgaag atgttacgcc gagaaattaa
     5941 caaatagggg actgtgacaa aagacgtgtc acagtccctt tttccgaata aacagtgttc
     6001 tcctgccaac tccttgtgac tcggagctga atggcaggct cacaacctct tggtaaacgg
     6061 tgttcaaaag ccagtttttc ggtattaagc caaatttatg caaaaatgta gaaagccatt
     6121 ttcacaaaat ttttcttact actcgaaact aagcgacttt ttcacaacct cctttacata
     6181 tttttatttt gttgtggaag gggtttcgtg ttattctaaa agaggaacga ttgaaggtta
     6241 taggaaagga agagatgaat ggaacacaaa gaacttacct catttccaaa agattttctg
     6301 tggggctctg cttctgcagc gtatcaagta gaaggagctt ggcaagaaga tgggaaagga
     6361 gaatcagtct gggatcgatt tgtacggata cctggaaaga catttaaagg aacgaacggt
     6421 gatttggcag tagaccacta tcatcgttat aaagaagata ttgctttaat gaaagaacaa
     6481 ggtctgaaag cttaccgctt ctctgtagct tggacacgga tttttcctaa tggacgagga
     6541 gaagtcaatc aagctgggct cgctttttat gagagattga tcgacgaatt gattgaaaac
     6601 gatattgaac ctatgttgac tttatatcat tgggatcttc cacaagcatt gcaagatgaa
     6661 tataatggtt gggaatcacg tcagatcatt gaagatttca ctcattatgc agaaacatta
     6721 tttgaggcat tccgcgggaa agtccattac tggattagtt tgaatgaaca aaatatcttc
     6781 acttcattag gttatttgtt agcagcacat ccgccaggtg tgacagaccc gaaacgaatg
     6841 tatgaagtta accatattgc taatttagca aatgcatctg tcatcaataa atttcacgag
     6901 ttagaaatgc ctggaaaaat cggaccaagc tttgcgtata cgccaaatta tccaattgac
     6961 agtaatcctg aaaatgttct ggctgctgaa aatgcagaag acctgatggc caattattgg
     7021 atggatgtct atatgtgggg aaaatatccg attgctgcca tgaggttctt agaagaaaaa
     7081 ggatgggcac caacgattga agctggcgat gcagaattgc tagaatctgc taaaccagat
     7141 tttcttggga tcaattacta tcaaacggcg acgaatgcat tcaatccatt aaacggagtt
     7201 ggtgctggaa aaatgaatac gacaggaaaa aaaggttcat cagaagaaac tggagttcct
     7261 ggaatgtata aaaaagttga gaatccattt gtcgaacgaa caaattggga ctgggagatt
     7321 gacccagaag gattaaggat cggactgcgt cgtatcacaa gccgttaccg catgccggta
     7381 ctgattacgg aaaatggctt aggagagtat gacaagttga cagaagataa acaaatccat
     7441 gacgactatc gaatcaatta cttacaaagc cacgtcaaag ccatcaaaga agcaatttcg
     7501 gatggagcag aagtcctagg ctattgcacc tggtcttata cagatttgct tagctggctg
     7561 aacggttatc aaaagcgata tggatttgta tatgttgatc aagacgaaac acaagaagga
     7621 tctttggaac gatataagaa agacagttat tactggtacc aaaaactgat tgaagaaaat
     7681 ggagagaacc tttagttcac atacttaaaa caagaaggta cggctggata tggccgtgcc
     7741 ttttcttgat aaaatcattt agaaattaag aaaaactgta aaataagctg aaaaatgaaa
     7801 gaaagcgtca gttctttcaa acaaaacttt agttccctta cttttttttg tataatgaaa
     7861 cttgatgaaa tgagggataa taaatggcac aaaagaaacg gcctactaaa aaaaggaaaa
     7921 agacgaaaaa aacgaaaaag caaaaacagc agcaggaaca aatgctgacg attggtgttg
     7981 ggtttgcctt gattttattt gctgtcttcg gctttttaaa attaggtttt ttaggaatat
     8041 tgattgcaaa cggctttcgg atcattgcag gaaatactta ccaaatcctc tgtcttttat
     8101 tagccgtatt aggattttgg attgtcatta agaatacaga gttttcaatt ggtaaaaacc
     8161 gacgctggtt tggcggcata ctcttttatt tcggtatctt attattgtta catgcccatt
     8221 tatttggcaa actccatact ggggaaccaa atatcatggg gacgacatgg gatttgttag
     8281 ccagtgatat aaaacaaagt caagtagaca ataacgttgg tggtgggatg attggagcaa
     8341 tcctttatca ttttacttac ttcttgattg cacagcctgg aagttattta gcagcagtgc
     8401 tattactggc aggaggagca ttcctgttca gtaatctgga aggctatcag ctgttaaatg
     8461 gtatccaatc aattggagaa cgagtacagg aattattgga aggtgatcct gccaagcaag
     8521 ctcgaaagca agcggcaaag gaagagagaa tgaaacagcg cgcagaagcg aaagaagcaa
     8581 ggcgcttagc tgcaaaagaa gctgcagaaa aagaagctgc agaatatgag aagaaaaaag
     8641 cggtgagcca gcaaagggaa aatactgctg atgaatggca ggaaccagaa aatacagagc
     8701 cggagcagct aagttttgtt ccaatcgaca gttttcaaga aaatatccat cctgcgaatc
     8761 tagagaaacc agtaccagat acaccaaaac agaccaatac agcagaaggt ttttcagatg
     8821 agttgccaga ggatgacggt acctcattag aatttgagat tgaagcagaa caggaaaatc
     8881 aagactatga attaccgaca gttgatttgc ttgattctat accaactgtg gatcaaagtg
     8941 atgagtataa aaaaattgaa aaaaatatcg gcgtattaga acaaactttc caaagtttcg
     9001 gtgtcgatgc caaggtcgtc aaagccagct taggaccatc cgtcacaaaa ttcgaagtcc
     9061 agccggctgt aggggtaaaa gtaagtaaaa ttgtgaactt aacagatgat atcgcgcttg
     9121 ctttagcggc gaaagatgtc cggatggaag caccgattcc aggtaaatca ctgattggta
     9181 ttgaagtacc aaatggaaag atcagtatgg tttcctttag agaaatcatc gaagcacaac
     9241 caaaccaccc agataaatta ctagaagtac ctttaggacg tgatgtttct ggacgggtac
     9301 aaacggctga tctttcgaag atgcctcact tactcgtggc tggttcgact ggtagtggga
     9361 agtcagtagc aattaatggg attatcacaa gtatattaat gcgtgctaag ccccatgaag
     9421 taaaactgat gatgatcgat ccgaaaatgg ttgaattgaa tatgtacaac ggcatacctc
     9481 atctgttgac gccggtcgtg acgaacccta gaaaagcagc gcaagcctta caaaaagtag
     9541 tacaagaaat ggaagaacga tatgaaaaat tcgcggcaac cggagtaaga aatatttctg
     9601 gttacaatga atttgttcaa cagaaaaact tggaaaacgg aacgaaacat ccaaccttac
     9661 cattcatcgt agtgatcgta gatgaattag ctgatttgat gatggtagcc agcaatgaag
     9721 ttgaagatgc gattattcgt ttagcgcaaa tggctcgggc agcggggatt catatgatcc
     9781 tagctactca acgtccaagt gtggatgtta tcacaggaat catcaaagca aatgtacctt
     9841 ccagaatggc atttgcggta tcaagcggta cagattctcg gacgatcatt gattctaatg
     9901 gggcagaaaa gctcttaggg cgaggagata tgctattctt gccaatgggt gagaataaac
     9961 cgatccgtgt gcaaggagca ttcatttctg accacgaagt agaacgggtc gttcaatttg
    10021 ttacagatca gcaagaagct cattatgaag agaaaatgat gccgacagat gaagttgaaa
    10081 cgacaggtgc tccagaacag ccacaagatg agctgtttga agaagcaaaa gcacttgtcg
    10141 ttgaaatgca aacggcaagt atttcccttc tccaaagaag atttcggatc ggttacaacc
    10201 gagcagcacg tttagtagat gaactggaag ctcatggagt tgttggcccg tcagaaggaa
    10261 gcaagcctcg gaaagtattc attgagcagg aagaaacatc agaaacgttg ccggacgata
    10321 atgacatgta aaacttgtgt gatgaagggt tgaaaaacga ataaccaata aaaagacaag
    10381 gcatgatgat accaaaagaa aaaatggttt ttttccatct tttctaatac ggtatgatgt
    10441 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
    10501 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
    10561 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
    10621 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
    10681 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
    10741 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
    10801 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
    10861 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
    10921 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
    10981 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
    11041 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
    11101 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
    11161 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
    11221 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
    11281 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
    11341 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
    11401 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
    11461 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
    11521 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
    11581 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
    11641 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
    11701 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
    11761 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
    11821 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
    11881 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
    11941 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
    12001 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
    12061 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
    12121 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
    12181 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
    12241 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
    12301 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
    12361 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
    12421 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
    12481 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
    12541 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
    12601 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
    12661 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
    12721 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
    12781 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
    12841 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
    12901 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
    12961 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
    13021 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
    13081 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
    13141 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
    13201 tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta
    13261 tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat
    13321 tttgaataaa gtgtttctgt ataatttttt tattttataa gtaagataaa taatgaaaga
    13381 atagagatac tgattggtag ctaaagattt ttgagcgaga acatttcgtt ttttcaaaag
    13441 gactgttaga ataagcaaga acaaggggga agacgtatga taatcagtga atttgaccgt
    13501 aataatccag tattgaaaga tcagctttct gatttactga gactgacttg gccggaagaa
    13561 tatggagaca gctcggcaga agaagtagaa gaaatgatga atccagaacg aatcgcggta
    13621 gcagcggtag accaagatga gttagtagga tttattggtg caatccctca atacggtatc
    13681 acaggttggg aattgcatcc attagttgta gaaagctccc gacgaaagaa ccaaataggt
    13741 actcgattag tcaattactt agaaaaagaa gtagcttcca gaggaggaat cacgatttat
    13801 ttaggtacgg atgatttaga ccatggaaca acgttaagtc aaacggacct gtatgagcat
    13861 acatttgata aagtggcttc catccagaac cttcgtgaac atccgtatga attctatgaa
    13921 aaattaggtt ataaaatcgt aggtgtctta ccaaatgcaa atggctggga caaaccggat
    13981 atttggatgg caaaaacgat tattcctcga ccaaattctc aataataaaa agaggttgtg
    14041 acagaagtaa tcagcttcaa gaaataagaa ggaatttacg aaaattactt cttgaatttt
    14101 tgtgaaattc caacttattt ccgaagaagc tacttttgga acaccgtgta ccagaggttg
    14161 tgaaaaagtc gcttagtttc gagtagtaag aaaaattttg tgaaaatggc tttctacatt
    14221 tttgcataaa tttggcttaa taccgagaaa ctggcttttg aacaccgttt attcagataa
    14281 gggactgtga caagactttt gtcacagttt ctttttgttt attctttttt atctttgtat
    14341 ggcaataata gacaatataa cgaaaattat ttaatggctt ttggaagacc atggaaaacc
    14401 ttgtacaaag ctattttttc tgaaaaattc cttgaaacct cacgaaaaaa tcattatttt
    14461 atgaaaaaga caaaaaaaag tgttgatttt ttaataatta ttcattaaac tcttaaacaa
    14521 cttcagaaaa tattaattat tctgaaaatt ccaataaagg ggtacgtaaa atgaaaaaga
    14581 aatttacatt tggtttggtt gcggtatgcg gccttgtact tgctggatgt tatggcggca
    14641 gttcagccac aagcagtagt tcagcaaaaa gcggttcagc agatggagga ggcgtattta
    14701 acttagtcgt gcctcaagaa atgcctacag ctgacttatc agttgcaaca gatacaatca
    14761 gttttacagc attgaataac gtttatgaag gaatttaccg tcttgatgaa gacagcaaac
    14821 cacagccggc aggagctagc gagttggcag aagtcagtga agacggatta acttacaaaa
    14881 tcaaattacg agaagacgca aaatggtcaa atggtgatcc tgttacagca gcggattacg
    14941 tttatggctg gcaacgaaca gtagacccag ctacagcttc tgaatatgct tacctgtatg
    15001 cgccagttga aaatgcagaa gacattacag ctggaaagaa agataaatca gaactaggga
    15061 tcaaagcagt tggtgactat gaattagaaa tcaaattgac gaaacaaaca ccatatttcc
    15121 aatatctgct tgctttccca tcatttttcc cacaaaaaca atccgtagtg gaagaaaatg
    15181 gggatgccta tgcatctgca agtgataaag ctgtttataa tggaccattc acattagaag
    15241 attttgatgg accaggaaca gatacagaat ggacttataa gaaaaatgat cattactggg
    15301 acaaagatac agtaaaactt tctgaaatca aagtcagtgt agtaaaagaa tcttctactg
    15361 cattgaactt attcaaagat ggccaagcag atgacgtgat cttgtctggt gagttagcac
    15421 agcaaaacgc aaacgatcca gcttatactt ctgttaaaga agcgcggaca agctatatcg
    15481 aattcaatca acgtgaaaag aattcaccat tcaacaatgt gaacctaaga aaagctattt
    15541 cttattctat cgatcgtgaa gctttagtaa aacaagtact aggtgacgga tcagttgtct
    15601 ctacaggatt gatcccatca gatatggtaa aaagtccaac aacgaacgaa gactttgcaa
    15661 aagaagctgg aaaattagtt tcttatgaca aagataaagc aaaagaatat tgggaaaaag
    15721 cgaaaaaaga attgggcgtt gattctttag aatttgattt gatggcttct gatgatgatt
    15781 catcgaaaaa agtaatcgaa tatgttcaaa actcaatcca agaaaacttg gatggcgtaa
    15841 cagtcaaacc aacacctgtt ccattctctg tacgtcttga ccgctcaact tcgggtgatt
    15901 ttgacgcagt actaggcgga tgggctgctg actatgcaga cccaagcagc tttactgatt
    15961 tgtttgtcac aggtaattca tacaaccgtg gtcgctggag caatgcagaa tatgacgaag
    16021 cagttaaagc agcttcatca acagacgctg gcgatgaaca agcccgttgg gatgatttcc
    16081 aaaaagcaat caagatcatc tctgatgata tgggtgtcgt tccggtgtac caaaaagcag
    16141 aaggtcacct agtgaatgaa aaaatcaaag gtatcgttca ccatgcggca ggcgcttcat
    16201 gggattacaa atggacatac gttgaagaat aatcgttaat accgtgagca tgagaaaaaa
    16261 cgaggctgcg gcaaatgtcg caacctcgtt ctcatattca cttatcctaa caaagaaaaa
    16321 agcatgaaaa tagcagaaag agcaattagt attccggcaa tgatcagcca aaagctgtac
    16381 ataccgtagc tggcagacga gagcgaggaa aattctggtt ttttctttcg gttccttgtt
    16441 cgtgcaagat ggaccgatcg ctgaaaatga tcgaagaaac ccgaagaaaa gacccataaa
    16501 aaaccgccga taatcagaaa cggcaatgac cataagaata gatcattaga taattgaatg
    16561 agtgtaaagg aatggttgat gagatttttt attaaaataa ccagaatcag tagaaaagct
    16621 actgcgtagg ggatagattt ttttttcatt tgattgctcc tttatttttg gttcattatc
    16681 taataatgta gcgaaacatc tgtcagaagt caaaagctag ggggaaacat tagtgaatag
    16741 ttatatcaaa tatgtattaa aacgtgtctt tttcatgatc atcacgcttt ggttaattgc
    16801 aacaatcacc tttttcttga tgcaattgct gccgggcacg ccatatacga atcaggaacg
    16861 tttgagtcct gaaacaatcg agatgctaaa taaacaagtc ggtttggata aaccagttat
    16921 cgtacaatat gggatttatc tttctaattt gcttcaagga gattttggaa tttccttcca
    16981 atttaaaaac cagccagtcg cacatttgtt agctggaaga atcggaccat ccttgcagtt
    17041 agggttacag gcaattatct ttggaacagt cctagggacg attttaggaa cgatctctgc
    17101 catgaagcaa aatacatggg cagatacatc ttctacactc gtagcgatat taggtcgttc
    17161 cattccaaac tttgtatttg ctgttttatt acaatatatc tttgcaatca aattacaagt
    17221 tttgccaatc gcaaaatggg atggctttgt gtacacaatc ttaccaacaa ttgcgttggc
    17281 catgtcgccg cttgctgact ctgctcggtt tatccgtacg gaaatggtgg aagtactgca
    17341 tagtgattat gtggaattag caaaagcaaa aggattgagc cgttgggaaa tcgcctttaa
    17401 acatggtctt cgaaatagtt tgattccatt gatgacttta ttaggaccgt tagctgtagc
    17461 attgatgacc ggatcactgg tggtagaaaa tatctttgct attcctggta tcggggaaca
    17521 attcgttaag tcaatcacaa caaatgatta tcctacgatc atggctgtaa cgatccttta
    17581 ctcatttatg ctgatttttg ttattttgat cgtggatcta ttgtacggtc tagttgatcc
    17641 aagaattcgt gtttcagaag ggagccgagg ataaatggaa atgataccaa agcgttatga
    17701 aacagttgca gatatccctg cggatgaatt tcttccatta caaaaaaata ccgaagaaga
    17761 acgtgaacag attactgcac cttctttgaa ttttatccaa gattcatggc gccgtttaaa
    17821 gaaaaacaaa gctgccgtta tttcaatggt tttactattg gtcatcattt tgatctctgt
    17881 gatcacaatc tttgtttctc ctcatgatcc aactgcccaa aacgtggctt atatcaattt
    17941 gcctcctcgt attccgggaa tcaatatcga tggattgaat ggaaaagcaa tggttggtgg
    18001 tgaacttgtt gataaatatg ctcaagcaaa cgtcccttca aacgtaaatt tctttttagg
    18061 aacagacggt ttgggacgtg atgtattaag ccgcttattt atgggaatga gaatctcgtt
    18121 gctgatcgca tttattgctg cattactaga tgtaacgatt ggtgtagctt acgggttggt
    18181 ttctggtttg ttaggcggac gtgtcgataa cgtgatgcaa cgttttttgg aaatcttatc
    18241 aggaattcca aacctcgttg tcatgatttt gatgttggtc gtttttgaac caggaatttt
    18301 ttcaatcgtg gctgctatgg ccatcacaaa ctggatacca atggcacgta tcgttcgtgc
    18361 acaaacaatg aaattaaaag atcaagaata tgttttagct ggattgacat taggtgaatc
    18421 aagaatgaaa attgcattca aacatatttt gccgaatatc tctagtgtca ttattattca
    18481 aatgatgttt agtattccat ctgctatttt cttcgaagcc tttttaagct ttatcggttt
    18541 aggtcttacg cctccgtcag cttcattagg aacgatgttg agcgaagggt acaagacatt
    18601 cctgtatctg ccttatctat tatggattcc ggcagtaact ttatcggtta tcatgattgg
    18661 ctttaactta cttgctgatg gattgcgtga tgcctttgat cctaaaatga aagagtgaag
    18721 acgatgacga aaattttaga agtgaaagac ttagaaatct cttttgatac gtatgctgga
    18781 aaggtacgag cgattcgcgg tgtcaatttt catttgaata aaggagaaac attggcgatc
    18841 gttggggaat ctggaagtgg aaaatcagtg acgaccagaa gcatcatgcg cttgctgtca
    18901 agcaacgcca atatagatgc tggtcaaatc ctctttaaag gtaacgatat cgttggaaaa
    18961 tcagaaagag agatgcaaaa aatccgtggt aaagaaatag cgatgatttt ccaagatcca
    19021 atgacctctt tagatccgac gatgcccatt gggaagcaag tcgctgagtc ccttagaaag
    19081 cataataaag tttcaaaaaa agagggacaa aaagctgcat tagaattgct taaattagtt
    19141 ggaatacccg aagctgagaa acgtatcaac agttatccgc atcagttctc aggaggtcag
    19201 cgtcaacgga tcgtgatcgc gattgcactg gtatgttatc cggaaatcct gattgctgat
    19261 gaacctacaa cagcgcttga tgtgactatt caagcacaga ttttggagct gctgaaagaa
    19321 attcagacaa aaatcgatac atcaattatc tttatcactc atgaccttgg tgtagtagcg
    19381 aatgtcgctg atcgtgtcgc tgtcatgtat ggaggaagaa tcgttgaagt tggaacatcg
    19441 gaagaaattt tctacaaccc gcaacatccc tatacttggg gactgcttgg ttcaatgccg
    19501 acattagata gtgcgaacga tcgattgtat gctattcctg gttctccacc ggatttattg
    19561 aacccgccaa aaggagacgc attctatcca agaaatgaat tcgcgatgaa aatcgatgca
    19621 gaacaagaac caccattttt tgaactatca aaaacacaca aggcagcaac ttggctactt
    19681 gctccgcagg cacccaaagt gacaccgcca gaagaaatcc aacgtcgctg ggctatcttt
    19741 aaagagaaac aaaaacatac ttacggtcaa ggaggaattg ctgatgccaa ataaactact
    19801 tgaagttaaa ggattgaaac agtatttcaa tgtaggaaaa aagaatgaag tacatgctgt
    19861 aaatgacatc agctttcaca tttatgaagg agaaacattc ggtttagttg gagaatctgg
    19921 aagcggaaaa tccactactg gccgtacgat cattcgcttg aatgaaccta ctgatggtga
    19981 gatattattc gacggtcaag atataacgaa gatcaaagat aaaaaaggtt tgacgaaatt
    20041 ccggcatgat gttcagatga tctttcaaga tccatacgct tcgttgaatc ctcgtatgaa
    20101 agtcagagat atcattgccg aaggaattga tgttaatgga ctagctaaat catctgaaga
    20161 gcgtgcaaaa aaagtcgatg atctattgag aactgttgga ttgaatccaa gccacggtac
    20221 tcgttatcct catgaatttt caggtgggca acgccagcgt attggtattg cacgagcatt
    20281 ggcagtaaaa ccgcgtttta tcatttgtga tgaaccaatt tctgcactag atgtgtcgat
    20341 ccaagcccaa gtggtcaatt tgttgcaaga tctccaaaaa gaacatcaat tgacctatct
    20401 atttattgca catgatttgt cgatggttaa acatattagt gaccgaatcg gtgtaatgca
    20461 taacggcttg ttattagaga tgggaacaag tgatgaaatc tataatcacg gtgttcatcc
    20521 ttatacagaa agtttgttat ctgctattcc tctaccagat ccagaccatg agagaaaacg
    20581 cagaagaatc aaatatcaac ctgaaccaga tgacggacaa gtacgtcaac ttcgtgaaat
    20641 cgcaccagaa cattttgtct atgcaactga gcaggaagtc ccatattacg caaaaaaact
    20701 aaaacgacaa aaggaatctc ttctggttgc taactaggag ggacatagat gctaaaagct
    20761 tttaaattcc gtatgtatcc gacagaagaa caaaaacagc aattgattcg tacttttgga
    20821 tgtgctcgat ttacctataa ccatttgctg aaaaagcggc agaaaagttg gcaacaaaca
    20881 ggagtagcca atttttcgct cacacccgct acattaaaaa aagaataccc attcttaaaa
    20941 gaagtagaca gtcttgcttt agcaaatgca cagcttaatt tggatcgggc ttttcgcaat
    21001 tatttcaagg gacgtgccag cttcccaaag cttaaaacga aaaaaagtat gtggcaatct
    21061 tatacgacca acaaccaaac ccgtacgatc tatctggaaa atggctactt aaagttacca
    21121 aaacaaaaag aactaatcaa gataaatcag catcggccag ttgaaggatc gattcgctcc
    21181 gctacgattt ctgctcgata taatgaagaa ttttatgtcg cactactttg tgatgtttcg
    21241 ccagtaaaaa aagaatcttt agctaaatgg atcgggatcg cctatcatcc gaagacattg
    21301 atccaaacat ctcgacctct agaagtaact ttaccaaaat ttcaccagac agaagaaaag
    21361 ctgcaacatg cacagcggaa attgaatgtg aaagtccgct ccgctcacca tagaaagatc
    21421 aggttagatc aggcaagcaa ttatcagaaa caaaagagaa aagtaatgga tctttatctg
    21481 aaacaaaaga accagcgaga agattatctt gaacaattat ctgggaaatt ggtcaaacaa
    21541 tatgactact tatttgtaga atcttttcca aaagaagagg cacatgctga tttttctatt
    21601 cacgactggc ataaattaat aacaaaattg cgttataagt cccaatggta taataaaaaa
    21661 tttcttttga tcaataccga cggagcagaa gaaagcaatt ccgtaagaaa gagccaagta
    21721 gtagaaaaaa tgggcagaca ttcggttatc aagggataaa aaatgaatga aactgatagg
    21781 agcgttaggg ttagccttgt taataaaaag tcttaatgac ttttgtttct aggaattttt
    21841 cttttgaaga aaagctcact aattatgaca aaccaataaa acaatagcag aaaaagttcg
    21901 tacgagcttt ttctgctatt gttaattttt tattcagaaa gtttacgaat acatcaaaat
    21961 tctgaaacaa aaaacgtaac tcacaaagag ttgtttttgc gttttttctt gaatgattag
    22021 gtctgaatct tttgctcaca accttattca tggtgccccg gcttgagctg atagattttt
    22081 gaaggacggc cgattcctac aggacgttca ccgatctcat cgaaatattg gagcatagct
    22141 ttcttaaaat tggaatgatc gatcgtttta taatcgatcc ctaagaattt agcaaatact
    22201 tttcgagctt ccgtaatcgt gaaatcttta cctaaaactt gtagaacttg cggctcgtgt
    22261 tccattttat tgactacacg gttgaatgct ttcaagatga tttgactatg gtcaaaagcc
    22321 aaagtatctt tgccagaaga ctctccagtg actaaatcaa gcgagatagc cacttctccg
    22381 ctagataaat ggatcttgtt actatgacgt tctagtgtga accagcgtac ttccttggca
    22441 tcatctccgg cgattagcgg ttcttctcca ataaaggcta agtagctgac tgtgacgacc
    22501 cagcctcgcg gatctcgatt aggcgtactg aatgtatgca gttgctcaat attttctttt
    22561 gaaataacca caccagtttc ttcctttgtt tctcttaaga cgctttctcc agttgactca
    22621 tccttttgga caaagccacc aggcaatgcc catgaatttc gataaggatg cccttttctt
    22681 tggatcaaca aaactttcag ctgatcttct tctttgttat agcacatcag tacgatatcg
    22741 acagtcaaag agggcttgtc atattctgga cgttcctgtt tttgatacca gttaagaaat
    22801 tcttcttcac ttgctttatg ttcataatat aatttttctt cttctttaga agcaaaacgg
    22861 ttcatctgac cacctccatt gacagtaaaa gtacttttta ccttattgta gcagactctg
    22921 aaaaaaaacc aaggatttac ttgaaaaatc taggttatca tttaaatatg aaaaataaat
    22981 ggaagtatga gattgaataa aaagaaaagc agataaaacg gcaatacttt ataccgctct
    23041 tcacgaatgg caaagaagcc acaaataacg atatatacgt caatgataaa acgcaaaatc
    23101 caagctgctg tcataaaact gttcaaatgc ccatggaggt taaaataacc atacatacga
    23161 atataaaaat agctgaatat taaatgtgct aatgaaagaa aagcaatact aagtgtaaaa
    23221 aaaagatgtt tcttctgcat gattgttcca gtcccttact aagatagata gtctttatta
    23281 taccacgtgt gaggggagaa ataaaaagcc gttttttagt agatatgaaa caaaacaaaa
    23341 acccgcattt attcaaccta ttcatccttt taaaggatag attgaacgta gatagaagtc
    23401 gtttatccag tctttttcat tttgaaaaaa agctaacagc atgctttttt tatggtatac
    23461 tagcaaggaa acgaattcgc atcatgcgat aaaggaggaa gagccccgtg gcagaaaagg
    23521 aaacttttta tatcactacg ccaatctatt atccaagcgg caaacttcat atcggcaact
    23581 cgtatactac gattgcatgt gacgcaattg ctcgttataa acggttgatg ggctttgatg
    23641 tgttttatct cactggagtg gatgaacatg gtcaaaaaat cgaaacaaaa gcagaagaac
    23701 taggtgtaaa accgcaagaa tatgtagata aaatggcggc tgatgtcaag aaattatgga
    23761 aaacgcttga tatcagttat gataaattca ttcgcacgac agatgattac cataaagcag
    23821 ctgttcaaaa aatatttgat cgtttattag aacagggaga tatttactta ggtgaatatg
    23881 aaggctggta ctctgtttct gacgaagaat tctttacaga aacacaactt gcagaagtgt
    23941 acaaagatga cgaaggtaac gtgatcggtg gtaaagcgcc aagtggacac gaagtagaat
    24001 tggtaaaaga agaatcatac ttcttccgca tgagcaaata cgcagaccgt ctattggaat
    24061 actatgaaga acatcccgaa ttcatccagc cagaatctcg taaaaacgaa atgatcaaca
    24121 actttatcaa accaggatta gaggatcttg cagtttcacg aacaactttt acttggggag
    24181 taccagtcaa gagtgatccg aaacatgtgg tgtatgtatg gatcgatgca ttatccaatt
    24241 atattactgc actaggatat ggctccgaag atgaaagtct attcgaaaaa tactggcctg
    24301 cagatgttca gatggtcggt aaagaaatcg ttcgctttca tacgatctat tggccaatca
    24361 tgctgatggc ccttgatttg cctctaccta aaaaagtatt tgggcatggc tggctattga
    24421 tgaaagacgg aaaaatgtct aaatcaaaag gaaatgtcgt ttatcctgaa atgcttgtgg
    24481 aacgttatgg tttagatgca ctccgttatt atttgcttcg tgctgttccg tttgggtcag
    24541 atggtgtctt tacgccagaa gattttgttt cccgtgtcaa ttatgatctg gcaaatgatc
    24601 tagggaactt attgaatcga acgattgcta tgatcaacaa atattgtgat ggactcgttc
    24661 ctgactatgc ctcacttgtc actccatttg atagtgaact gtctacgacg gctgctaacg
    24721 ttgtcggacg ttaccatgat gcaatggaaa aaatggaatt caatacagca ttggctgaaa
    24781 tctgggtgct gatttctcgt gcaaacaaat acattgatga aacagaacca tgggtattag
    24841 caaaagacga agaaaagaaa gctgaattga atagtgtcat gatccattta gctgaatctt
    24901 tgcgtatcgc agcgatatta cttcagccaa tcatgacaga aacgccagag aagatcttca
    24961 atcaactagg attggactct gaaacaatga atcttgaagg attacacttt ggcgaatttc
    25021 cttcaggaac aaaagtagtt gcaaaaggta caccaatctt cccacgttta gatatggaag
    25081 aagaagttgc ttttattcaa gaaaaaatgt ctgaaggtac tcaaacaaac gaagatacag
    25141 tcaaatggga tccagaagaa acagaacttg tttcaacgaa agaaaaacaa atcaaatttg
    25201 atgtatttga aaaagtagaa ctaaaagtag cagaagtcat caactgtcaa aaagtagaag
    25261 gggcagataa gttgctgcaa ttccgactag atgctggaga cagccaagac cgtcaaatct
    25321 tgtcaggaat cgctgaattt tatcctgatc caagcgaatt gatcgggaaa aaagtcgtca
    25381 ttgtagcaaa tctgaaacca cgaaagatgc gtggacaaat cagtcaagga atgatccttt
    25441 ctgcagaagc accagatggc tctttacaag taatcgaagc accaaaatcc atgccaaatg
    25501 gctcagaaat cgcatagaaa ttcaaagggg gtatgacact agttgtgtca tacccccttt
    25561 ttccgaaatt tgagaagcaa ttttcgtaaa ttccttctta ttacttgaag ccaatcatat
    25621 aatacagaaa cagagaacta tccaaaataa atcctagaaa aagaagtacg agcttcgctg
    25681 atttttgtta atttagaata atactaatag gaatcgtaaa aacagaaaaa aagcataatg
    25741 ataaagggtc gggcagattt taattgattc atctggtact cttttttatt gtccttacat
    25801 tcataaagtt tgaatgcaat gactttatta catctatatt tatatctaac agtagcgttg
    25861 atgtgttaat tataagttct atcactttat gttcaattag tttgtgctct tatatgtgtg
    25921 attgaaaata tagataaaaa aacaatcatc ggctttgact tcgatgattg ttttctttat
    25981 tttgtaaaaa tcctatcact tgattttatg gtaacctttt atttttgtct tcaaacggct
    26041 tggtaagaga gcagtaagca gtgcaaatgg gatcgaaaag acataccaga aaatcgtacg
    26101 tccgatacca taatgttgat agagctttgt caacatcacg tgtaacaaga tcaaccctaa
    26161 aagcaaggag ccagctataa accaatgatt atcccaatgc atgacttcac taagtgccat
    26221 cactccaaaa ggatagaaat aaaaatagat cccgactaag gtcatccgcc agttctttgt
    26281 aaatgttccg atattcaata aaaagaaaat cgctgaaagc aaaataccga taggcggaaa
    26341 aagcccaacc aaaaaggaat acaacatccc ccacaagatc atcacaggac ctttgatcaa
    26401 tgcaacgatt gcgatgaaca aagtcagcaa tattagtttt tgtgataacg ttgcaacagt
    26461 cattaatcca aataaaatta atgtgaacca atgccaatgg gactgctgtt gttctttacg
    26521 ggaaaaatcg acccggttgg ataaataatc tgctaatttc ggataagaac gcttaaaatc
    26581 cataaactgt tcctccttcg tggaaaaggt aggtttagta tacgaaaaat aaaaaagaaa
    26641 tacatccgtc ttaagaggga gtttttcact gaaagtggag aaaaaagaat ggtaaattaa
    26701 aatattctca gttatactga aaattatacc aaatttcaaa gaagaaagag gatgggaatg
    26761 aaaggcaaac taaaacgaga aatcaatctg tttggcgcat tagctaccgt tatgggaacc
    26821 gtgatcggtg caggcgtctt cttcaaaact gctgctgtta ctgctagtac tcagtctaca
    26881 agtttgacat tgctagcttg gcttttgggt ggtttcttaa cgatctgtgc tggattgaca
    26941 gtggctgaat tagctacagc aatcccggaa actggtggag ctgtaaaata tattgaagct
    27001 gcgtatggaa aattgcccag ctttttatta ggatgggcgc aaagcttgat ttactttcct
    27061 gctaatattg ctgcattatc gattatcttt gcgacacaat tgacgaactt attacaatta
    27121 tctaccgatt atcttttgtt gattgcagtg atcaccgctg tttctgtgac agggttgaat
    27181 ttgttaggga caaaagtcgg tgcttctgta caatcagtga cgttgattgt taaattaata
    27241 ccaattgctg tgattgtcat atggggatta ttgaccccag gacaaggaac tgtccagtta
    27301 tttccaatcg aagctggaaa agatgttact tttgtcgaag gattgagcag tgcgttgtta
    27361 gcaacgttgt ttgcttatga cggctggtta ggtgtcggtg caatggcagg ggaaatgaaa
    27421 cggccggaaa aagatttgcc taaagcaatt attcttggtt tgagttttgt aacagtcgtt
    27481 tatctattga tcaattttgt ttttttgaaa actttgccga ttgatcatct tgccggtaat
    27541 ttgaatgcag cttctgaagc atcagacgtc atttttggag gaatcggtgg aaagctggtc
    27601 actattggga tcttgatctc ggtatacggt gcattgaacg gttatacact aactgggatc
    27661 cgcgtgcctt atgcgatggc tttagaagac gagctacctt tcagtaaaca gctgacaaag
    27721 ctttcaaaga agtttacggt tccttatgtt cctgcggttt tccagttagc agtagcttgt
    27781 attatgatga gccttggttc tttcgacttt ttaacagaca tgttgatttt tgtgatgtgg
    27841 ctattcagtt tactgatttt tatcggtgtc tttattttaa gaaaaaaagc gccggaattg
    27901 ccacgtccat ataaagtacc actttatcca atcgttccga ttattgcgat tctcggtgct
    27961 attttcattt taggtatgac gatgatgacg caaacgaaat tagcattgat cggtataggt
    28021 gttaccttga taggtatccc agtctattat caaaaaaaga aaaaacgctc agaagagtaa
    28081 aatatttcaa caacagacag aaaaagtacg aactaggtcc tctagatctt cgtgcttttt
    28141 ctatttcaat aaaacaagtt cttctatttt gatttttgct acttctgcaa gtagtgctta
    28201 tgtgctaaag taagaatgaa aaggagagat aaacatgata tttgattctc atacccattt
    28261 aaatgctgaa cagttcaatg aagatattcc agaaacgatt cagcgagcac aagaaatggg
    28321 ggtaacgaaa atggcggtcg tgggcttcga tacgccaacg attgaaaagt ctttgcaatt
    28381 aagccatgac tatccaaata tatacagtat tatcggctgg catccgacag aggctggcag
    28441 ttatacgaaa gaaatcgaaa ataaattaca agaacaattg actttaccaa aggttgtagc
    28501 attaggggaa attggcttgg attatcattg gatggaagac ccgaaagaag tccaagataa
    28561 agtatttcgc agacagattg ctatcgcaaa agaaatgaac ctaccaatca gtattcatac
    28621 tagagaagca cttgaagaca cgtatcaaat cctaaaagaa gaagatgttc gagatatcgg
    28681 tggaatcatg catagtttca gtggtgatta tgaatgggca aaacgatttc ttgacttagg
    28741 gatgcatatt tcatttagcg gtgtcgtcac tttcaaaaaa gcattagacg tccaagaggc
    28801 tgcagcaaat gtaccaatcg aacgattact tgtggaaaca gatgcccctt atctggcacc
    28861 agttcctttt agaggaaagc gaaatgaacc aggctatact cgttatacag tagaaaagat
    28921 tgctgaactt cggaatcttc cgacagaaga agtggctgcc caaacatgga caaatgcgca
    28981 ccgattattt ggtctaagcg aacatggata agatgaagat ccaagagatt atagtcgttg
    29041 aaggaaaaga cgatacaaga aggctgcggg aagtagttga tgcagatacg atagaaacaa
    29101 tagggtctgc aatcaatgaa gagatcctga tgcaaattga acatgcccaa gagactcgtg
    29161 gagtgatcgt ttttaccgat cctgattttt caggagaaaa aataagaaaa atcattatgg
    29221 aagctgttcc tcaggcaaaa catgcttttt tgccgcgcac acaagctaaa ccaaagaaaa
    29281 agggaagcct tggtgtcgag catgcaagtg atgctgctat tttagaagca ttgaaaaagg
    29341 tcgtgacgcc agacatttcc caaacggctg tttctgaaat cactagacaa gaccttttag
    29401 actatggttt aatagctggg gcacaagcaa aagaaaaacg agaaaaacta ggtgacgagt
    29461 tgcgcatcgg ttacacgaac ggcaaacaat tggaaaaacg tttgaagatg tttcgtatta
    29521 cgccgaaaga actcgcccaa gcaatgaaaa tagtggagga acaacatgag tgaatatcgt
    29581 gaaattgcga caccctctag aacaaaagag attttattga aacatggttt ttcatttaaa
    29641 aaaagtttag gacaaaattt tctgactgaa ccaaatattt taaggaaaat cgtagaaact
    29701 gcgcaaatag atgaacatac gaacgtgatc gaagttggac caggaatcgg tgcattgacg
    29761 gaacaattag ccaagcatgc aaaacaagtc gtagcatttg agatcgatga tcgtttgatc
    29821 cctgtattgg ctgatacgat gcaaccctat gacaatgtga ctattgtaca tcaagatatt
    29881 ttaaagaccg atttgtcaac agctgttaga gaaacatttc atgaggagct tccgttaaaa
    29941 gtcgtagcga atttacctta ctatattacg acaccgatca tgatgcattt tcttgaatca
    30001 gatttggttg tggatgaact agtcgtcatg atgcaaaaag aagtagctga tcgtatctct
    30061 gctgaaccag gaacgaaagc ttatggttca ttatcgattg ctgttcaata ttatatggaa
    30121 gccagtttag cattcatcgt accaaaaaca gtatttgtcc ctcagccaaa tgtagattca
    30181 gcgattttga aactgacaag aagagatata ccggcagtcg aagtgacaga cgaaaaagca
    30241 tttttccgat tgacaaaagc ggcatttcag caaagaagaa aaacgctttg gaataacttg
    30301 cagcattcgt atggcaaaga tgaccagaca aaagcatggt tagcaaaaag cttggaaaca
    30361 gctggaatcg atccaaaacg tcgtggggag actttgtcat tacaagaatt cgccgcatta
    30421 agcaatgcga tgtcagaaaa tatgcagtaa aaaaagaggt tgtgacagaa gttgtgaaaa
    30481 agctgtcctg catcaagtaa taagaacagc caaacaaaaa tagttcctca tgtttttcgt
    30541 ttgtttgggc ttattaccgc agatgcaagc ttttgaacac cgtttaagaa agaggttgtg
    30601 ggcctgccgt ttaactctaa gtcacaagga acaatttcaa aaaacagctt ctcatgtttt
    30661 tgagttagtt ggtcttatta cttgatgcaa gcttttgacc actattatgc cgaacaagga
    30721 gatgtgacac aaattttgtc acatctccta tttttgtttc ttgatcaaaa aattcttata
    30781 cttgatactc acactcattt tgtatggatt tagaaaagaa tatgtgtctt gacagttatt
    30841 agtaagtgag ttatactaag tcgacattta tgtgtcaaga catattagga gagagaaaaa
    30901 atgagaaaaa attcagttcg aaaactaacg atttctgctt tattgattgg gatgggggtc
    30961 attatcccaa tggtcatgcc taaaatcatg attccaccag cttcctttac tttggcgtct
    31021 catgtgccat tatttattgc gatgtttttt acaccaggag ttgcagtggc tgttgcgcta
    31081 ggtacgacat ttggtttctt tttaacaacg ccggttatca tcgcattgcg agcattgtca
    31141 catcttgtgt ttgcgttgat tggggcttgg tacttgcaaa agcatccgag tatcgtttta
    31201 aaaaatggac agtttacttt tgctaattgg cgtttccaag ggttcaattt tgtgatcggt
    31261 gtgattcatt ctttagcaga aatgctagta gtcagcatct tttactttat tggtaatatg
    31321 cctgaaacat attattctca aggatatttt tatacagtat tcatcttgat ggggattggt
    31381 gggcttatcc acagtttagt tgattataat attgcttatt ttatagcagg aacactcagc
    31441 aaatattttg atatccccgt atttacggca gcaaaaaaag aaaatcggaa agagtcgatt
    31501 caagggtacg cgaagaaaac gacatattag acatgatctc tcaaaaaaaa aactctcatc
    31561 tagtaatgga taataaatcc tctaagatga gagttttttg tcctaaaaag agctgcacca
    31621 acatcttctg gcacagctct aacagggggc aatttatgga agtttgatga cttgttctgt
    31681 ttctttatca aagaagtggc ctttgctgat gttgaaggca aggtcgacta tttcgcctgg
    31741 tttatggaaa tctcttgcat ctacttttga gatgaactca gtagaaccag tttttgtgta
    31801 aagcatcgtt tcagctccaa gtaattcgga aacgacaact tctgatttca ctacggcttc
    31861 aggtgaagct tcaagggcta cttgttcact gtggatgtct tctggacgaa taccgaagat
    31921 aacttctttt ccttcataac ctttttcaac taatactttg ttttttcctt caggaatacg
    31981 aagcttaagc ccatgtccat ctgtgataac accatttttc aatgtcacat taaagaagtt
    32041 catggcagga gatccaataa agccggcaac gaacatattg acgggtgtat cataaacttc
    32101 tttcggtgta ccgatttgtt ggataaatcc gtctttcatg ataacgattc ggtcagccat
    32161 agtcattgct tctgtctgat cgtgagtaac atagatggtc gttgtttcaa gacgctggtg
    32221 aagtttggca atctcagcac gcatggctac acgtaattta gcatctaaat ttgataatgg
    32281 ctcatccatt aagaagactt ttgcatcccg aacgattgcc cgtcccaatg ccacacgttg
    32341 gcgttgtcca ccagataaag cagctggttt tcgtttcaag tattctgtca gacctaagat
    32401 ctctgctgca ttgtccacac gttttttgat gtctgcttta tcatatttac gtaatttcaa
    32461 gccaaaagcc atattatcaa aaacagtcat atgaggatac aatgcgtagt tttggaaaac
    32521 catggcaata tcacggtctt taggtgccac gtcattcatg acttttccgc cgatatttag
    32581 ttctccttca gagatatctt ccagtcccgc gatcattcgc aaagtagttg actttccaca
    32641 accggaagga ccaacaaaaa cgataaattc acggtctttg attgatagat taaaatcagt
    32701 tacagaataa ttttctgcat tgtcatattt tttatatacg tttttcagag ccatttctac
    32761 cataatacgt tccacacttt cttttttagt tttattaact atttaaagta taaatgaaag
    32821 cgttaactaa cttcaatgtc atgttgaaca agaatttttt cttttttttg tcaatgtgct
    32881 agttttaagc tttaataaat atccataaga aataaaaact gatacactgg agtaaataag
    32941 tatgttaaca atatcaaaag gagtgtctct tatgaaaatc gctttgatcg ctcatgaccg
    33001 aaaaaaacct atgatggtga aattagcaac agcgtataaa acaatattgg aaaaacatga
    33061 actttttgct accgggacga ctgggcagag gatcacggaa gcaaccggct tgccgatcca
    33121 tcggtttaaa tccggaccgt taggcgggga tcagcaaatt ggagccatga tttcggagga
    33181 tcagttagat ttggtgattt ttcttcgaga cccgctggct gcacagcccc atgaaccaga
    33241 tgtaacagca cttatccgtt tatctgatgt ctatgagatt ccgttagcta cgaatatcgg
    33301 aacagctgag gtgctactaa gaggactgga agccggcttc ctagattttc gaagaatcgt
    33361 ccatgaaata gacaagcatc cactttcatt ttgatggata agcgtatgga aaaataacgt
    33421 ttaatgttcg cctatatgta gctataaaag ctaaacaaac tgataatatg cgatataata
    33481 cgaacgtatt ggataatata aacaataata ttagtttttc gtatttgcag taaatgtgaa
    33541 aatatgctat agtttctgtg ggaaaagatc ccaaaaataa tacagagtag aaaataaagc
    33601 gttaagtgct aagaggatgg gatgttgcct cttggacgaa ggacgtaatg ttcgcggttt
    33661 tattttcgca ttcgctgcat gatacgatgt agcagctctt aaaggagatg ctaaaaatga
    33721 agaaacaccg gttagacgca cgtatgattg ctattatggg cttattgatt gcattgatgg
    33781 tcactttgtc ccgactggta gcaatcgaga ccccatttat taaaatcagt gtcacattta
    33841 ttccacaagt gatcatgggc attctttttg gtcctttttg gtcagggata ggcgcagttt
    33901 tagctgattt agtaggaatg gccctcttct cgaaatctgc attctttatt ggatttacat
    33961 tgaatgcatt tattgaagga gcaatctacg gattcttttt ctatcgaaaa gagatcactt
    34021 ggaaaaatgc cattctggct acgcttagtg tcacgctgat catcaactta tttttgacac
    34081 cattatggct tgcgttgatg taccatgtgc cattgttcag ctgggtcgtt tgggcgcccc
    34141 gcctgttaaa aacagtaata tggttaccga ttcaatcaat tgccatttat tacgtaggac
    34201 gtagtattcc ttacaaaaaa attttaagaa gtttagcgat ccacgctaaa taaaaaacaa
    34261 gcagtcttcc catcccagaa gactgcttgt ttttattgtg ctgtgaattg tgcttgatag
    34321 agccgtcggt aataaccatc tttttgaagc agctcatcat gggtgcccat ttcgacaata
    34381 gctccctgat ccatcactaa tatctggtcc gcatttttga ttgtggacag gcgatgggca
    34441 atcacaaaac tggtttttcc ttccatcatt gctaagaagg cttgttggat ttttttctct
    34501 gtcaatgtat cgactgagct ggtagcttca tctaagatca acataggggg attactgatc
    34561 atcgttcgag caatcgtcaa cagttggcgt tgtccatctg atatctttaa cccttgaccg
    34621 ccaatcttag tatctaattt ttccggcaaa cgttctacaa attcatacat ataagaggct
    34681 tttagggcat cattgatttc tgcgtcactt gcttccggat tcccaaaacg aagattttct
    34741 cgcaatgtac tgtcaaatag ccaagtatct tgtaacacca ttccaaaact tttacgtaat
    34801 gtatcacgtt ggatagagcg gatgtcatgg tcatcgactg taattgttcc ttttgttgtg
    34861 tcataaaaac gcatcagcaa attaacaagt gtggattttc cggcccctgt ttttcctact
    34921 atagcaatgg tttgacctgg ataagcagta aaattgaaat cttcgatcaa acgctgcttg
    34981 gggtcataag aaaaagaaac atgatcaaaa gagacttttc ctttgatatt tttgggttcc
    35041 aaggcatagg catctggcaa atcaggcttt tcttctggct gatcgatcaa atcaaatgct
    35101 cgttctagac cagaaaaagc ggtttgcagt tgtgtcgtga ttccagaaag ttcgataaat
    35161 ggtttagaaa actgacttga gtagatcgta aagctggaaa tcacaccaac agtgattgac
    35221 gaaccactct tcaatgccaa taggccgcca actaaaccaa tcgataaata agcaaggtga
    35281 tctacaaaac gagaagacgg gtttgtcaag gaagaagaaa attgtgcccg ttgtcctttt
    35341 tcatatagat cagcatttag cagttcaaat tgctgttgac tagcttcttc ttgctgaaaa
    35401 gctttcacga ttttctggtt accgattcgt tcggtcacaa agcccgagat atccccaata
    35461 atcctttgct gtctatcgaa atttgtttgt gaagcacgag caacgatcca actcacaagg
    35521 aaaataattg gtgtagaaca aagaacgacc aatgtcagaa gtggacttag acgcatcata
    35581 aaaataaacg aaagaatgat gattgccact ccagaaaata attgattgaa ggccgcagaa
    35641 attgcaatgg aaatattgtc catatcattt gtaaatcgac tgacgatatt tccgtgggag
    35701 ttttgatcat aataacgaag tggcaatcga ttcagatgag caaatgcatc ttttcttaat
    35761 tttgcaacgg ataagtaagc taaccgattg cttaacactt ggatgagcca ttgactgact
    35821 acgttgatta aaacgatacc accgaataga ataagtaaat ggaacagtcg attgaagcgg
    35881 acctgtcctt ctccaatcat ttgatcgata ctttgaccaa tttgtaacgt catcacaact
    35941 gttgcaatcc cattgatgat tcctagtatc acagctagcc aaatgtcttt tgcataggca
    36001 gtaaggtaag gaatgaatcg gcggagcgtc tgcaaggata atttttttac tttcataact
    36061 attcttcctc ctgtgatgcg acgatttcct gatattctgc tgattgagca agtaattctt
    36121 catgggtacc ctgagcagca atcttacctt gatccatcac taagatatga tgtgcttctt
    36181 gaatagagcg gacacgttgg gagataataa tcactgtact gccaagataa gtctgtagcg
    36241 ccttacgcaa atccaagtct gtttgataat ccaacgcact taaagagtca tctaaaatca
    36301 gtaatgctgg cttggcaatc aaagctcttg caatcgtcag tcgctgtttt tgtccgccag
    36361 agaaattttt cccattttcc atgacttttg tatctaaacc atttggaagc tgacggacaa
    36421 attcttctgc ctgagcgatt ttcagagctt cccaacattc tttttctgtg gcgtctttct
    36481 taccccattg aaggttttca cgaatagtcc cggtaaatag tacagaagtt tggggaacaa
    36541 gagcaatttg ctgacggaga tgatccagct gccaagaacg aacatccatt ccgtccatta
    36601 taactgtacc ggaactgaca tcatagaaac gcggaatcaa ttgtgtcaaa gtcgttttcc
    36661 cgcttccggt tggaccaatg attccaagga ctgtatttcg cttcagctga aatgtaatat
    36721 tttggagaga aagaccagaa tcaggagtat aacggaagtc aacatggtca aaagcgataa
    36781 ttgcttcaga agaaaaatcg ggagtagtac tgacctcagc ttcaagaatc gaattttctg
    36841 tgtctagcac ctctttcacc cgatttcctg aagcttcggc acgtgtgaag atcacgacga
    36901 ggtttgaaac aacgatcagt gccaaaagca tttgattcat ataattgatc aaagcaagaa
    36961 cttgtccttg ttgcaagcta ccaatattta ctttgatccc gccaactgtc agcaaacaga
    37021 tgatcccgac attcatgatc aaggttgtag caggtgataa tagggcagag atattggaaa
    37081 cacgtaaata attgttcgct agatcatttg tgcttttatc gaacttctct gtttcttttt
    37141 ttgtctgcgc aaatgcgcgg atgacacgaa caccgctaag attctgacta acgtgttcat
    37201 tgagctgatc tagtttttct tgcacttttt ggtagagagg aacggttttt ttgatgataa
    37261 aaaataaaat gattgagaaa ataggcaata atgctaagaa aaacagacct acttcccagt
    37321 cgatcacaaa agccatgacc actgacccga tacttaaaaa cggtgctctg atcaataaac
    37381 gaatgaccat tgctaaagct agctgaagct gattgatgtc gtttgtcatt cgggtgatca
    37441 aggtatctgt tccaaaatga ttcaactcgt tgtgagagaa agtattgatt tttttgatta
    37501 gctgattacg taactctgta ccaaatcctt gtgaagctac tgaagcatag tattggcaaa
    37561 tgatagcaca aatcagacca atcacagaca tagcgaccat ccataagccc atttcaatga
    37621 tttttcctcg gtcattcatt tttagtccat tatcgatcaa ggaagccatc attaaaggca
    37681 ggacaagttc gaagacagct tcaagaaatt tgaagaacgg gcccaaaatg atttgcttac
    37741 gatagtcttt tgcataacgt aataactgaa tcatatgatt cctcctatta tttatttgtc
    37801 cacaattgaa aaaacaagta ctaccacatt gtagcttagg aaatttagaa tgaaaagaga
    37861 acaagccttt tcataaatga aagttttatt atgaagaagc tttagaaatt gaatagacta
    37921 ttttttttaa aaaaaagaaa atgagaaaac atgccagtct cttcattcat aggaacgtct
    37981 attttactta atttttctat aaatgaattc tgcataaaaa atatatcaag taaatgttta
    38041 tagatgaaac tctatttact tcttttttaa tttgttcttg caatcttttt tgctacgtaa
    38101 tttttgtaaa aaagtgggtt gagagaaatc taattgattt tcaaagaagg agtatgaaaa
    38161 tgagaaaaat atttattcgt ttattgtggg tactatctta gaggaataaa taatagtgga
    38221 gaaaactatg gtttggcgca gattctatca ggtttgatgg aacgaagaca acctcccgac
    38281 atcaagtcaa aagctacttg agattttgat ctgaaaataa aaaaagaaaa caaggtagga
    38341 acatcaatgt ttctaccttg ttttcgtaat caatgggaaa aaactaagag tggtgtacgt
    38401 aatcaataaa aaggatggtg acaacagtac ctctttactc tctgaaagca tcataactcg
    38461 tttttcctca ttatgccaat aaattgttgt tataccacat ataaggattt gtaatagcta
    38521 aaaacagaca ggaaagttga aatctgtgaa tcttattttg tatggaagga tttatagaac
    38581 gcaaaaatca agataaagat gaataaaaat cctgtttcgg ctatatattg cgtatctacg
    38641 ccaataaaac gagtttgttc tgtcatagcc atataaagca aaggcaaaaa tacaactaca
    38701 gcgagataag atccgatgat ttctcgaaaa gttttccagg aagtatgttt cctgttttta
    38761 tatttatccg atttcattga atagtcatag cctccttatt ctttcatatg ttgaaattcc
    38821 agtataaaaa ctggaaggaa ctgtacgttt tttagaataa aaattataag ggataagtag
    38881 gctgctgtct ataataagat atatttttca attctttatt aaagatttta ttgacaaaat
    38941 gaatccgttt tctatgatgg aggaggggtg aataagttgt tgaagaagga aattttatgt
    39001 tacttagaga accagacagc ctttttcgat ttagaacata tgaacgaggt ctttacagca
    39061 caaaggttag ctgatcattt tggggtaaaa agaaacacag tcagtcatta tttgaaccag
    39121 ttaaatgatg agcaactact agtcaaaatc aattctcgac cagtgattta tttccacaaa
    39181 gaagcttttg aaaaacaatt ttttaagtta cgcacgaatt tttatttttc tatcgatgat
    39241 ctcaaagagg aacagccttt tttcgcgcaa ccaaaagact tattctcttt tatgatcgga
    39301 tacgacgcca gtttgaaaga aagtatcgaa caaatcaaaa cagctcttta ctatccagat
    39361 ggcggattac cacttttgat tacaggcgaa agtggcacag gtaaaagtta tttagttaat
    39421 ttggtctatc agtattgtct gcttcatgat ctgctagaag attctgcacc attcatcact
    39481 tttaattgtg cacaatatgc agataatcct gaacttttga ctagcaattt gtttggacat
    39541 gttaagggag cgtacactgg tgcagaagaa aataaaaaag gggcttttga agcagcagat
    39601 ggtgggatct tgtttttaga cgaggtacat cgcttaacac cagaaggtca ggaaaaactg
    39661 ttcacctatc ttgatcaagg tgtgatttat cgtatgggag aacctaattt gtcacgccgg
    39721 atcaaaacac gtctcttttt tgctacgact gaagagatta ctagtcattt cttaacaact
    39781 ttcattcgaa gaatacctat ccgaatcgaa ctacctgcac ttaatcagcg tagtcgtaat
    39841 gaacggctag aactgattta ctcttttttt ctcgaagaac aaagaaaaat ggggagccct
    39901 ttgtcagtca gtggaccagc tttgtcactt ttaacaagtc agaaattatc cggaaacata
    39961 ggagagctga aaaatatcgt caaagtttca gcagcaaaag catatgctga acagcgtgaa
    40021 cagcctgaaa tccatgtcac gatccatcat ttgcaaaaag aactacttgc gcagcctatt
    40081 actaaaaata cagcacagca aggaatctat attacgcaag accagacctt agagcaatta
    40141 atggagaagc aacagcccga acagcagcga atcgttaaaa gttttgagca aatcctaatt
    40201 gatttcaaga aaaataactg tctgctatat tcttgcgaag gaaaattaaa gcaagaagtg
    40261 attcaattat ttgatttttt gctgtttgag accagtagac aagaaaaaca cgagctgctg
    40321 gtttattgga cacaatctat ccgagagacc tttcgacaaa tggaaactgc ctatcaaatc
    40381 aaattcgatg gaaacagtgt ctatgccttg agttactatc tctatcagcg aagcactatc
    40441 aaatggttac cggaagaaca tgatattatc caactaatca aagaattaga acaacaagtt
    40501 tctcagtctt atccatctag ttatcactat gtccaacgaa ttctggaatt aagtaagatc
    40561 aaattagata ttgaagacac aagtatggat cggattttac tgacgatcta tctaaaaaaa
    40621 gcgaaatggt caaaagaaaa acgactgcca aaagcgatga ttgctgctca cggttatgca
    40681 actgctagca gtatggcaaa tgtagtaaat cgtttattac aagaggatct atttgaatca
    40741 tttgatatgc cactagatgt cacaccacag cagattgctt cagaaatcct tgattattgc
    40801 gaaaacagcg atgtttcaaa tggattgatc atattagtag acatgggatc tctgaaagaa
    40861 atccatcagt tttttaaaaa acagttatcc gtaccgcttt tgattttgaa taatgtgacg
    40921 acacctttag caatcacagt aggggaatgc ctgcaaaaaa atgtatcatt agaagagata
    40981 gcagaagaga ctgttcgaca gatccagcca gaatggcgac tgctctatcc agaggaaaac
    41041 aaacccaaag cactcattac tacttgcttc actggaatcg gtacggcggt ccacttaagt
    41101 gaactgctag aaaaaagctt acctacggct tgtcagttga aaatcatccc atatgaatat
    41161 cagcaattaa aagataagaa aaacagtgac ccattatttt ccatatacga agtcgtagga
    41221 atgattggca ccacagatcc tgatatcacc aatatcgact atctttcatt agaagacctc
    41281 atctcggggg aaaagatgtc tgtactagct gaatggttgg acactactat gaatgcttca
    41341 gagaaggaaa tattcaatca gcgcatcatt cgtaattttt cattggaaaa agtactggat
    41401 tccgtgacta ttctagatac agagaaggtg atgggagaaa tcgatcattt tatgagagaa
    41461 ttagaggtgc agttgaatcg aagcatcagc aatcccaaaa aattagcgtt gtatgtccat
    41521 gtgagctgtc tcattgaacg attgatccga caaatgccaa tcgaaaccta tcaaggatta
    41581 gagaaatgga agcagtgtca aaaagaaggg ttatctgcta ttaaatctgc gtttagtgtc
    41641 attgaagagc attatagtgt catgatcccc aattctgaga ttgcctatat ctatgatatt
    41701 ttgagtgaag atacagagtt tctctcgata gaagaagaat tttgaacata agcaagttgt
    41761 tgccactaat tttggcatgc aacttgcttt tttagtatgt gtaagtgaaa agaggagatg
    41821 aaaaagtgga tagagaaatt atcttagctt cccatggaaa atttgcttca gggatattag
    41881 attcattaga gctgatctat gggaaaaatc attctattac tgtattcgat tgctatactg
    41941 atgagaattt tgatttagcg gaaacagtaa ggcagctttt tatgaggtac aaagataaag
    42001 aaatcattgt tctgacggat ctttttggcg gaagtgtcaa caacgaattc ttacaatata
    42061 tcaatcggga tcatgtctat ctcgttgccg gattgaattt gccactattg attgagctag
    42121 tatctcggtt ggacgctgaa gtagagacaa ctgttttgat ccagcaggtg ctaactgatt
    42181 caaaaaaaat gatccagttt tgtaacgagc ggctcaatca gaaaatagaa gaagacgaat
    42241 tttaggagga agaaaaatga ttgtattgac aagagtcgac catcggttat tacacggaca
    42301 agtagctttt tcatggacac aaacaatagg cgcagactgt attttgattg ctaatgatga
    42361 tgtacctacg aatgagatac gaaagacaac gatcaaatta gccaagccac aaggagtcaa
    42421 actagtgatc aaatcaattg atgactcgat tgccgcgttg aaaagcgggg tgacagataa
    42481 atataagtta ttcatcgtag tggaatcgat cgaggatgca tataagctgg cagaagcgta
    42541 tccagaaatc aaaaccatca acttaggagg gatcaaggca aaacctggta ctcgcagcat
    42601 cggaaaagcg gtaaatattt taccagaaga ggaggaacgc ttgaaacgat taattgacaa
    42661 acaaatagaa gtagaaatcc gtcaagtacc agcagataaa aaagtggatg taagagaagt
    42721 tttgtaagaa aggaagaacc tcaatgattg tacaagcagt cttattagga attgttgcat
    42781 ttgtcgcaca atcagaatat gcactaggga catcgttgct ttctcgtccg attgtgacgg
    42841 ggctgtttac aggaatcgtg ttaggtgatg taaaaactgg gatcattatg ggcgcaactt
    42901 tagagttagc atttatcgga tcgttctcag taggtgcttc gattccacct gatgttgtga
    42961 ccggtgggat tttagggacg gcttttgcta ttacagctgg agccggtaca gaaaccgcgc
    43021 ttttactagg tttgccgatt gctacgttga cattgatatt gaaaaatatt tacttagggc
    43081 ttttgattcc aattatgaac cataaagcag acaattatgc agaagaagga aattataaag
    43141 gagtcgagca tatgcacttg cttgccggta tcggtctttc cttgatgctt ggtgttgtgg
    43201 taatggtttc ttatatggtg ggaagcaata cgatcggaaa tctattaaat atgattcctg
    43261 acttcgttca aaaggggttg tcagtagcta cagggttgat tccagctctt ggttttgcga
    43321 tgcttgctcg tttgctgatc aacaaacaag ttgcaccata ttttttctta ggttttgcta
    43381 tagcttctta cttagagatt cctgtgaccg gagtagctat ttttggtgcg attttagcag
    43441 ttgtggtagt caatattatg aatgtccgtc aactaccact tcaaacaact gcaggggagg
    43501 tagaagaaga tgaagaattc tgagatcaaa aacagcaaaa taacgaaaaa agagctaaat
    43561 cacgtatttt ggcgttcttt tcaaatggag ttttcttgga attatgaacg tcagatgaat
    43621 atggcttatg tttatgcaat gattccgatt ttgaaaaagt tatatcagtc gaaagaagaa
    43681 atggcagcag ctttgaaacg gcatctagag ttttttaaca cgaccccaca tatcgttaca
    43741 ctgattttag gaatcacggc tgcaatggag gaagaaaata aggatgatcc tgaatttgat
    43801 gtgactgcga tcgatagtat caaaacatct ttgatgggac ctcttgcagg gttaggagat
    43861 tctttctttt ggggaacttt gcgattgatt gccacaggtg tgggtacctc tttagccatg
    43921 caaggaaata ttttaggccc tatcttgttc attttgattt tcaatattcc gcacattctg
    43981 tttcgctatc tagcaacagg ttggggatat cgcttaggaa cgggtttttt gaaaaagatc
    44041 caggcaaacg gtatgatgga aagtttgaca ttaggcgcgt ctattattgg actgatggtc
    44101 gttggagcaa tgactgccac gatgatcacc atcaatattc ctttaaaaat cggttcagga
    44161 gaaaatgctg tgactgttca aagtattttt gatgatatcg ttccaaatat cttatgtctt
    44221 ggagcatttg gcatagtgtt ttatctattg aagaaagaag tgaaaccatt aacgatcttg
    44281 atcggactag ctttctttgg aatttttggt tcgtggatag ggatttttta aaaaaggagc
    44341 agagaaaatg tttacgatgc aagattatat ctatgaagaa aaggaagtac taagtacgat
    44401 cttgaaaaag aatgattttt cgacaagaga acatatgaaa aaaacagtga atttactgat
    44461 cttagcaacg ggttcatctt ataacgcttg tttagcagcg aagccagcat tggaatctta
    44521 tggtgatctt actgtcgata ttcaagaacc tttttatttt ttcaattatg gaaaactttc
    44581 tccttcaatc gatactgtga ttgctgtgtc acaaagcggg aaaagtgctt ctactgttga
    44641 agcagtaaaa atgatacaaa aacaagggtt acctgttgtt gctatcacaa atgacgtgca
    44701 aagtccactt gctttagaag ctgcacaaat tattgattta ggtgccgggg tagaatcagt
    44761 aggcttcgtt acaaaaggct attctgcgac tgttttacag ttactactct tagggatagg
    44821 aattggaata agtaaaaata aaatcagcaa aaaaatagaa caagactaca tgcaacagtt
    44881 gcgcaaaata atcaaccatt taccagcgat tattcaaaaa acggaagaat tttttgatgg
    44941 atatcaaagt ttatttcgtt tagcacagcg tttcgtcacc attggatatg gtccaaactg
    45001 gggaacagct aaagaagcag agacgaaatt gactgaaaca atccgcgttc cgtcacaagg
    45061 atttgagtta gaagcttata tgcacggccc atacctggag gcagatgctt cgcatttgct
    45121 attctttatt gaaggggaaa gtgtaaataa ggaacgttcg caaaagttgc aaagatatat
    45181 gagtcggtac gttggagaga ctctcaccat taccacaaaa aaagcaagaa acgagaaaac
    45241 acttggatta gcaatagaat gtgatgaata tctgtctgta ttagcgcttg tcgttccgtt
    45301 tcaactattc gcttataaaa ccgcagtagc taaaggtatt gacttgaaca aaaagatttt
    45361 tgaagacttt gatacagtct tgaaaagtaa attataagag gtggaaatca tgctgaaatt
    45421 caatgaagaa gaacaaatca aagcaataaa aggcgcacta gctttacgtc cgcaagtaga
    45481 agaaatcatc gataaacttt atgcggaaaa atttgacgct gtctattatc taggaatcgg
    45541 tggaacgtat gcttcctcga tgcaagctgt cacttatatg aatgggaaaa gcaacttgcc
    45601 tgtctttgta cagcatgctg cagaatatta cactactgga aacaaaagat taacaaaaga
    45661 ttctatcgtt attttgtcct ctgttaccgg tacaactcaa gaagtagtga aagcagtaga
    45721 acaaatcaag aaagtaggag cgacactgat tggatttatc gataaagcga acagtaagtt
    45781 gtctcagcta tgcgattttg tggtcactta tccagctcct ggaacagaac aaatcaaatt
    45841 ttttatggca gcagatcgat tgatgtattt gaatggagaa ttcgaagctt attcagaata
    45901 ctatgaacag ctcgaacgtt atttaccaac agggttagtg gaagcagaga aaaaagcaga
    45961 tgcatttggc ttatcatttg cagaaaaaca tcgacgtgat agtatgcatt attttattgg
    46021 agcagggaat caatggggag ctgtttattc atatgccatg tgttactggg aagaacaaag
    46081 ctggcttcca tcgaaatcta tccatgctgc cgagtttctt cacgggacgt tagagatcgt
    46141 agaagaaaca accccagtca cattattttt aggcgaagat gaacagagag tgttatctga
    46201 acgtgtggct aaacttttgc caaaaatttg cagcaattat acattgatag acacaaaaga
    46261 ctatccagta gaaggtatca gtgaaaaata tcgcggacgt gttttgtctt tcttattgat
    46321 gcatgtagtg acacaacgca tcgatgctca tgtggagcag ttgaactgcc atccattaga
    46381 gatacgccgc tactatcgtc agtttgatta ttaggataga ctggataaaa agaatataaa
    46441 aaataaagag agcagcgata actgctccct ttattttttt aaaatattgg catcaattac
    46501 gttataaaac acattttttg cattatctac agttcaactt ataatctgtt taatgggaca
    46561 tgtgggtgcc aaccattttt tgttatgcaa tagtactatt tagttgttgc atggatccgt
    46621 agtaaaagtc caagtacaac gagttgcgcc agtttacata gcgttttgta tccagaatgt
    46681 tgactaaaac aaaagttcct atgtcaaccg tcaacagaga caatctgtcc gttgcaggac
    46741 ctgacagatt tagaaatgtt caatctgtct cttatgtatt ttaaatcaat actcactagg
    46801 agaagtttcc taacctccta atagtaatgc tggtagtaac attgtcaaaa ctgcttcctc
    46861 ttaggaacaa gcaaagcata acgagtgtaa aaataaaatg tagactattg taacatatca
    46921 ttgattattt caagtaaaaa cgataaaaga agtaaacaaa cacttaaaaa caatggaaat
    46981 tagtcccaac tttaagaaat gtatactata atggaaatta tcaattattt atttggagga
    47041 atatccatga aacaaaaaat cgcagttctt ggtcctggat cttggggcac tgctttagca
    47101 caaacattgg cagaaaacgg gcatgatgtc cgtatttggg gaaatgtacc tgaacagatt
    47161 gatgaaatca ataagtatca tacaaatcag cattttttac ctgatttgac gatcccagaa
    47221 tcgattatcg gatataaaga attggcagaa gcagtggata acgcagatgc tattttattt
    47281 gttgttccaa caaaagcgat tcgttcagtc gcacaggaac ttattggtaa aatgaatact
    47341 aagccaatca ttatacatgc aagtaaagga ctagaacaag atacgcataa acgaatctca
    47401 gaagttttgg ctgaagaaat tccagaagaa aaaagacagg caattgttgt attatctggt
    47461 cctagccatg ctgaggaagt ggcagtacat gatatcacaa caattacagc tgcaagtatt
    47521 gatcaaaaag cagcagccta tgtacaaaaa ctgttcatga atgattattt caggatttat
    47581 acaaataatg atgtgattgg cgtagaaact ggtgcagcac tgaaaaatat cattgcaatt
    47641 ggtgccggcg ctatccatgg attaggattc ggagatgatg caaaagcagc aatcatgact
    47701 cgaggattgg cagaaatcag ccgtcttggt gtcgcgatgg gtgccaatcc tctgacattt
    47761 atcggtttga gtggtgtggg ggacttgatc gttacttgta caagtgttca ttctcgcaat
    47821 tggcgtgccg gtaacttgtt aggacaggga cataagctgg aagaagttct agaaaacatg
    47881 ggaatggtcg ttgagggtgt ggcaacgaca aaagcagcag tagagctagc tcaacagcta
    47941 ggtgttgaga tgccaattac tcaaacaatc tataatgttt tgtataatgg agaagatatc
    48001 aaaaaagcag caaaagaaat catgttacgt gacggaaaaa tggagaatga attttctctt
    48061 cgttaaatag atgatcaata gttaatcgtc gtttaggagg aacagcaagt gaaggtcaaa
    48121 aaagcagtca ttcctgccgc tgggctagga acacgttttt tgccggcaac aaaagccatg
    48181 gcaaaagaaa tgcttccaat cgtggataaa ccaacgatcc agtttattgt ggaagaagca
    48241 ttggattcgg gaatcgaaga catcttgatc gtaacgggaa aagaaaaaag acccattgaa
    48301 gatcactttg atgcaaatat cgaattggaa aacaatttgc gggaaaagga aaaaacagaa
    48361 ttactcgctt tagttgaaga aacgactcaa gtcaatcttc acttcatccg tcaatctcat
    48421 ccaagaggac tgggggatgc cgtgcttcaa gcaaaagcat ttataggaaa tgaaccgttt
    48481 gtcgtcatgc tgggggatga tttgatgaaa gatgatatcc cattgactca acaattgatg
    48541 atggattatg aagaaacaca agcttccgct gttgccgtca tgcgtgtacc agaaaatgaa
    48601 acatctaaat acggaattat tgatcctaaa acagatagcg aaaagggtcg ttgcagagta
    48661 aaagggtttg tagaaaaacc tgaaatggga aaagcaccaa gtaatttagc cattatcgga
    48721 cgttatctac tgactcctaa aatatttgag atattagaaa ctcaagaacc aggagcagga
    48781 aacgagatac agctaacgga tgcacttcag actcttaacc agacagaggc ggtctatgcg
    48841 agagagttta aaggaaaaag atacgacgta ggcgataagt taggatatat gaagacaaat
    48901 attgaatatg gacttcaaca ttcggaaatt gggacttcat taagtgcata tattatccag
    48961 ttgagtaaaa agctaagaac agaaagttaa ctggaaataa gtggcctgct gagaatgaaa
    49021 ggctatttgt tattaaagcg gcagcttgtg cgtcatttgc gcaactgtcg ttttagtttt
    49081 ttgactctag atcagttgag tcgtggaaaa cgaaagatag aaaaagactt tcgtgaatag
    49141 attcagaaaa tattatgcta atggtttaat ttttttttaa gttgtataaa agaaaatgat
    49201 tacataagag aaatgaaagc caagccagaa gattcatagt agaataaaaa ggcgaatttt
    49261 ttaattataa aaaaaggtgt gtgttcacta tgggaaaatt agtcgctgtt gcacagtcgc
    49321 tgcttgatat actcaagtgg ctgatggaag gaagcaattg gatatttgtg ttgccactat
    49381 tgcttattag tctatttatc atgtttcgca tggtcagaaa aaaagaaacg tttctgattt
    49441 ttatctggta tgcagcatcc gttggggttg ctaccttgat ggttgaattg gcgttaggta
    49501 gttgggaagc ttgcgtattt gctttatttt ttgctgtact gtatctaatc atttatatcc
    49561 catatatgaa aagaaagtct cggataggca gaaaaggtag acatacaact actttttcta
    49621 gtacagattc tctcgaacaa agtagaagaa cgagaagaag acggtaatat ttagtaggta
    49681 tacgcaaaaa aagataaaat aatggataaa ctattgagat gagagattta tttaattggt
    49741 ggaaaaaagc ttttttcact tatataaaag aatctttttt aaaggaatgc ttgttgtatc
    49801 gggataagcc attccttttt ttatgttatt tatatatata taaatcatac caatgttttt
    49861 ttgtattttc ttgcttgcac ttggataaga gattacttac aataaattca gatagaaaca
    49921 gctgaataaa aaaaggctcc ctgaccctta gttggcgcta agaatcaggg aatgaccagt
    49981 cacacacata caccctgacc gatcattttg ccatagtcaa tgcagtgcat cgacctaatt
    50041 tattgtacct aatctagggt gaaaattcta cccctatgta gatattttcc atggtctgca
    50101 cgttgtttta tttgctatgt caaggtattg ggcgtattcg ttcaatgcct tttttgtgtt
    50161 tgaaacagca aagaccacat gtgttactgt ctaactactg tttttttatg tacttcctta
    50221 gattaggcag ctgtttctat tcatgtcaag tatagctagg cgggagagat aaatgagttt
    50281 atgttatttt gactattatc accaattgga agagaaatta aggatagaag agccgctgat
    50341 gcttcaagct ggcacttgga tcgcgaaaca aattaatcaa ggtggtagat tgcagatata
    50401 tgctagccgc acactaagtg gtgtagcatt tgaattttgg gatcaaaccc cggatcttgt
    50461 accaagtata ttgattgaaa atccagccgg aggtgtctat gaaacgctag aaggtgcagg
    50521 acaggcaatt attgatcagc tttctgtcaa atcagaagac atttttcttc ttctttcgaa
    50581 tgaaggaaga aacccggcaa tcatcgaact agctcagtgg atcaaagaaa atggtcatcc
    50641 attaatcatt gttactggat ttgatttgtc acgaagtatc aaatcgcaac atagcagtgg
    50701 tttgcgatta tttgaatttg ctgatttggt tttggacaac cacgccaatt tacatgatgc
    50761 agtcttgact ttgccaaatc tagatccagc gatctgcgga acagcctctc ttgcaacgat
    50821 ctttttgctg cagcaagttt tgtattttac agcaaaacag ctagtccagg acacaagtgg
    50881 aaaatagtta tccacatgtt gacaaagttc cttaaaaaaa taatgttcca cataaaaatg
    50941 gagcgtttga aaaaaagaaa acgattatta atgaggatta acaacgacta ctttttttgt
    51001 tttagttatt ttaagatgga tcatctttga ttttaagctt gttttgttta aagtttgtta
    51061 tttttataag ttgtttcaca gttatgagaa atgagttata agaatatgta gtgtcagaaa
    51121 aataaaaagt agtggaaaaa gagacaagaa agaatccata tggattcttt cttgtctctt
    51181 aatacacgtt gatgaaagag tcttccccaa aactctttac aaagtaatta tagtacattt
    51241 gttatcatta ttgcaatgaa gtttacctga tataacaaaa ttgtccaaca ggataccttt
    51301 ttctaataga aataaaatac acaaaaattg aaggatagat aatggatatt tataagtgtt
    51361 tattattttt tttgcaatga aattcaattt ttcaatgatt gtaaaatgaa aaaaacagga
    51421 cgagattttc tctaatctaa aaataaaaaa agaattttaa ggaaaaacag agaaataaaa
    51481 tacttaatat aaatggaaag agaaaaaaat aatcatacat aggagaaaaa aaacaaaata
    51541 gaaaaaagtg attttctaat aaattattca tgtggaaatg tcatttttac tgtataattc
    51601 atttgattat ccatttttta ttcatcaaaa ttactgaaaa aagaaaggaa gaaataaatt
    51661 tgaagaaaac aaattcttca ggaaaaccga aacgcgactt cactaaatta tcgacacccc
    51721 atacgtatgt cattattttt ggtgtagtca tctttgcatg gatactgact tttgtcgttc
    51781 cagcaggaaa gttcagcacg caagatattg aatacaaaga tgcaaatggc gaaacaagta
    51841 ctcgtactgt tttacgtcaa gattccttcc gttatgctta cgagctggat aagtcgtatg
    51901 tgtttgatca attagaagaa ctgcaagatc atccagcaga acgtgaaaaa ctggatgttc
    51961 cggaaaaagg attggaaaaa gtcattgctg acggagaaaa aaatcttacg caggaaaaac
    52021 tagatgaaat ctccttgaca gatgatgtgc tttacgatca gtacggcgaa aatatctatg
    52081 atacttctaa aaaattgcat aaaacagcca aaatatgggg aacagatgat tttggcggct
    52141 ttggtttctt aaattttgtt tttgaaggac tagtatctgg agataagtat ggatctgctg
    52201 tagggatcgc tgcattgatc cttgttgttg gaggtgcttt tggtattatt atgcgtaccg
    52261 gagcgatcga cgcaggtatc tatgcgttta tcagtaaaac taagggactg gagcgtttag
    52321 ctttaccgct attattcttt gctttttcat tcggtggcgc tacattcggg atggcagaag
    52381 aggtaattcc attctcaatg gtgatggttc catttgtcat tgcgttagga tatgactcga
    52441 tcgtagcagt aaccgtgact tatgtcgcat ctcaagtcgg aaatgcgacc tcttggatga
    52501 gcccgtttag cgtagcggtg gctcaaggga ttgctggaat ccccgtgttg tctggtgcga
    52561 cattccgatt gattatgtgg gtagttgtta ctgcattagc cgctggttat atgatgatat
    52621 atgcagaaaa aatccgtaaa aaaccagaaa gttcgttgac ttacaaatca gatgattatt
    52681 tccgttctca tatcaaaaag acatctgatg aaaataaaaa attcatgctt gggcataaat
    52741 tgatcttatt agagatgtta gttgtccttg tctggatcgt ttggggcgtg actcaaaaag
    52801 gctattacat tccagaaatc gcttctcaat tctttgtgat gggattagtt gctggaatca
    52861 ttgctgttct tttcaaatta gatggaatga aagtcaatga catcgcttct tctttccaat
    52921 ctggtgcagc tgatttagct ggtacagcaa tcgtagtagg tatggccaaa gggattcttc
    52981 ttgtcttggg aggatctgat gcatctgttc cttcagcttt gaatacgatt ttgcatggga
    53041 tcgggacggc gttgaccggt gttccagctg tcattggtgc ttgggcgatg tacatcttcc
    53101 aaagtttgtt caacttggtt gttacatcaa attcaggaca agcagctttg actatgccaa
    53161 tcatggctcc actggcagat ttagtaggtg tatccagaca agtagcagtt cttgcttatc
    53221 agcttggttc aggttttatg gacgctttta cacccgtatc agccagcttg attggtgtgc
    53281 taggtgttgc acggatcgaa tgggcaaaat gggcaagatt ccaaatcaag atgcaaggat
    53341 tcctgtttgt tttaggaact attttcatca tgattgccat tgcaataggt ctgcagtagt
    53401 actggagacc tgtaaataaa aaagtaatcc aaacagtaga taaggactcc aagaaccgtc
    53461 attttacggt aaaatcaaag gagtaaagat cctatcgttt ggattatttt ttaagatcct
    53521 atcatcagtt atacttaaag aaagaggaaa aatgtatgaa aatcattcga caaattgaag
    53581 tgtttgcacc agattactta ggaaaaatgg atgtactgat tgccggtggg aagattttgg
    53641 ctgtagaaga acagctagaa ggcggctacg aaggagtgga agtagaagaa ctttcaggtg
    53701 agggaaaagt actaaccccg ggtttcattg actgtcattt ccatatttta ggtggaggag
    53761 gagaaggcgg ttatcaaaat cgaacgccag aagtcacatt gagccaattg actacagctg
    53821 gtgtaacgac agttgtcggt tgtttgggaa cagacggaga aggtagagat atgactgcgt
    53881 tgatcagcaa agcgaaaggt ctggaagcag aaggaatctc cacttatgta tacgaaggtt
    53941 cgtaccgatt gccggtaaaa acagtaacag attctatcat caaagacttt ttgacaattg
    54001 ataaaattat cggaatcgga gaaatcgcag tatccgatca ccgttcttct cagccaagtt
    54061 ttgaagagtt cgctcgtgca gtagcagatg ctcgtgtagg aggcatgtta tccggaaaag
    54121 ctgggatcat caatgtccac ttaggcggag gaaaaagaaa gctagagcta ttgacacgaa
    54181 tcgtagaaga aacagaaatt ccgatcacac aatttttgcc tactcatgca aatcgcacac
    54241 cagaattatt tgaagcttgc gtggcatttg ccaaacgtgg tggcacgatt gacttcactg
    54301 ctagcgaaga tcctgatttt tgggagaaga cagatggtga agttcgcttc agcaaagcac
    54361 tgaaacgatt gatcgaggaa gatgtttctt tagataattt cacgatgacc tcagatggac
    54421 aaggaagctt gccttatttt gatgaaaaca atcatttcct agggttagga gtaggaagtg
    54481 ccaaggcatt gctagtaggg atcaaagaag ctgtgcaaaa agaaagtatc cctttggaaa
    54541 tcgccttacg agctataacc agtaatccag cacgtatatt gaaattggat aaaaaaggaa
    54601 aaatcgaaat tggtgctgat gcagatctgt gtatcttgga taaagaaacg cttgatattg
    54661 atacagttat cgctaaagga gaaataatgg tccaagaaaa agaagtcaaa gtatggggga
    54721 ctttcgaaaa aagtttttaa tcaacagctc taactatctt ttttagaaaa cgagcgttct
    54781 gtttgtctgt atctgttgtt gatgttaccc tgaaggtaga tcaataaaca aactaagagg
    54841 aggaagcaca atggcagatt taaaaggacg ttctaaagat atgaaagata aagtagtcgg
    54901 tgaaggaaaa gaagcatacg gcaaagcaac agacgacaag agcaaagaaa cagaagggaa
    54961 agcacaatca ctaggtgctg acatcaaaga aaaagctcga aatttaggcg acgaagtcaa
    55021 agacaagttt tctcgaaaag acgaaaaaaa ataaaaacca cttattacta attgaatcaa
    55081 gcaaataaaa aatccgaacc atatctgcga gacagatatg gttcggattt tttatttgat
    55141 cattatcctg ttttttaaac ttcgattttt tgaaattttt tattttttag ttcaaagact
    55201 tcatcacagc tttttgctac ttcaatctca tgagtcacga tgataataca gcgattttgt
    55261 tcatgggcga tttcttggaa aagtttgacg atatcttgtg aagttgtttc atctagattt
    55321 ccagtaggct catccgctac gattagttga tgttcacagc agatagcacg aacgatagcg
    55381 actcgctgct gctgacctcc cgaaagtttg gtcacctgtt tttttgctaa tatttcatca
    55441 attcctacac gagccagtcg atctaaagca acttgtcgat aattctcttg cttggtctta
    55501 gtgatttcca ttgctgctat gacgttgtct agagccgata aataaggaag caagttgtaa
    55561 gcttggaaaa taatcgagac atcattttta cgataacttc ttaacccgat tttgttcagt
    55621 gaacgatctt gatagtatac ctttccttct tttggtgcat ctagtccaga aataagcgaa
    55681 agaaaagtcg tttttccaga accacttgct cctaaaatag cgtacatttt tccagcttcg
    55741 aaattcagtt ctacatcttt ataaagcgca tcttgcggat cttgatacca aaatcccaat
    55801 tgttctgtcc gtaacatcaa attcctcctt tagtttaata ggatttttct tgggtttaat
    55861 cttaatatcc ctatagaagc taaaaagaca gaaaacagac tgattccaaa tgctactccg
    55921 gctaatagtc ccaattgtgc aggtttgaca ttgatttcca attcgctgac ttgatcagaa
    55981 acagcaaatg gattattcgt ggttccttgt tgaccatcgc gttgaccgcc ttgttcttgt
    56041 ctgttacctg gcatttgtcc accgccagga gcttgctggt tttgtgtcgt ggtttctgtc
    56101 tgttggtcaa gtagctgctg tcccacggca ttcgcgacga cgtttccgct aagcgaagca
    56161 actcctaatg caagaatcat acaaatagcc acttcggtaa acaattggag aatgatttta
    56221 cttctggatt ctcctaaaga tagcaagacg cctaactcat gccgacgctc ccgaatggta
    56281 atcataatga tcagagtaag tatgataata ccggctacag ccactagtaa aacaacattt
    56341 ttagaaaaac ttgccacgtt gtttaaaggt tccaacattg actgatacat ggaatcattt
    56401 gattgtaagc taaatgtatc tgtgtcgatc agcttctcag cttctttgag aaactcatcc
    56461 attttatctg gatcagatag agtgtaaata gcagaatcta tcgtgttatc gtcagatgtc
    56521 ccatttagtt catttgcaaa agtgtaagaa gtgtaaagtg tattcgacgg attcatgaag
    56581 ttgaatttca tacccatact gccgcttgtt tcgctggatt cataaattcc tttgatcgtc
    56641 atttcataag tggtatcttc atcttcagta ctcgtgattg tgaaagtatc tccgactgcc
    56701 aggtcatttg tttctgctag agtagaatca atgactacat tgtttgtgcc tttgtcgctg
    56761 tcatctattc cttcaccatc cgtgattttt gctgttccat cagaaaaatt actattttgt
    56821 gcagttgctg acacaccact gatttgaaag tctgcttgag tcatttgcgg attcccgccc
    56881 attcctccag caggttcacc gcctgcatca gtagtttctg aactgtcttc acttgaatca
    56941 gaactagtga tggcagttat tccatctttt gccatagctg aggcggaact ttcaaaggaa
    57001 taactcttta cattatctaa ttcagctatt ttttccgcgt ccgttaagga aactggtgta
    57061 agagaaaaac tgcctggatc tggacgttct tcagtggtag aaccgcttga agaagtttgt
    57121 tcctgttttt taaatgaagc ttctcgattg gcacttaaag tcactgttgc tccaacttct
    57181 ttttgtgctt gattcgctgc ttcttctgca gcgcttcgaa tcgtgaggcc agctagcaca
    57241 aaaataagaa tggctgtgaa cacggcaaac aataacaatg aacgtcccca ttttactttt
    57301 gtacttttcc atgcacgttt cataaaattc atattttgca tccctcctag tagatgcatt
    57361 tgataataga caacctatct taaagctagc ttaaatctta tgctgtttgc acaccatcaa
    57421 catgaggttc tgtatatata aaaagtcact atagttattg caaaaaatgt ggtaaaatga
    57481 aaaaagtatg tttcaaaccg acgaagaaga aatgaaagag gaatagcaaa agaaatccta
    57541 gatgctacag agggattttt acgtgcatag ttgttctaac aattgctgat actagcgatt
    57601 tagtttattt gttgtataat aatggggaac agctgaatac ttatataagt attctggcta
    57661 tagcgtttcg caaaagatcc attttgttaa tgaagtatat ccaaaataaa atgcccatcc
    57721 aactgtcaga acgcggatgt gctggtaatt ttcctgctga ttctccataa gttgagaaat
    57781 agtgattttg catatgcttc attggaatgg ctcatatatt tagataaaga agaaaatcga
    57841 catctttaaa cttggatgtc ataagaagag gtgaagtttg tgagtaccat taaaattatc
    57901 ccattaggcg gcgttcgtga aaatggaaaa aacatgtatg tcgcagaagt gggcgaggat
    57961 atttttattt tagattgtgg gctaaaatat cctgaaaatg agttgttggg gatcgacgtc
    58021 gtaatccctg attttacgta tttagaagaa aacattgatc gtgtagctgg tgtcttttta
    58081 acacatggac atgcagatgc gatcggggca cttccttact tgttgtcaaa aatttctgta
    58141 ccagtattcg ggacagagtt gacgatcgag ttagcaaaat tgaatgtcag tcgtaatgaa
    58201 gcagccaaag gatttaagga tttccatgtg gtagatgagc atacagaaat agactttggt
    58261 gaaacagtag tgagcttttt ccgtaccact catacgatac cagattcagt ggggatcaat
    58321 ctgaaaacaa aagaaggtag tatcgtttat acgggagatt ttaaatttga ccaaacggct
    58381 atcccaatgt atcaaacaga ttatgcacga ttggcggaaa tcggcaaaga aggagtctta
    58441 gccttactaa gtgaatcggc aaatgcagaa aatcctcagc ctgtagcttc tgaagcccaa
    58501 atagcagatg aagtatttga tacgatcaaa tattgggaag ggcgtatcat cgtcgcttgt
    58561 gtagcaagta atttgcaacg agtgcaacaa gtattggatg cagcagaaaa agcacaacga
    58621 aaagttgtct tgactggtca agatttcgaa cgaattattc gcacagcgat gcgtttagaa
    58681 aaacttcaat taccaagtga agatctgctt gttacgttga aagacatgaa aaactatgat
    58741 ccagaagaac tgatcatttt agaaactggc cgaatgggtg aacccatcaa gtctctgcaa
    58801 aaaatggcaa acggtacaca ccgtaatgtc aagattgaag aaggagattt ggtttatatc
    58861 actactacgc caagcatcgc gatggaaacc atcgtggcga aaacggaaga tatcatttat
    58921 cgagctggtg gaacagtgaa gttgatctct gagaatatgc gcgtttctgg ccatgccaat
    58981 cctaatgact tgcaattaat gatcaatttg atgaaaccaa catattttgt gccagttcaa
    59041 ggagagtatc gtgaattagc tgctcatgca gatttggctc atgcagtagg gatgccatac
    59101 aaaaatatct atattactgg acgcggagac gtgctggaat ataagaacaa gcgtatgagt
    59161 gttgcaggaa ctacaagtgc agaaaatgta atgattgatg gattaggcat aggtgatatc
    59221 ggaaacattg ttttacgtga tcgaaaaatc ttgtcagaag acgggatttt cgtagcggtt
    59281 gtcactatca gtcggcgaga aaagaaaatc atttcaaaac ctcaaattac ttcacgggga
    59341 ttcgtttacg taaaagctag ccgtgatctg atgaaagaaa gcagcaatat gattgaaaca
    59401 atcgttgaga aacatttaga aagcaatgat tttgagtgga gcaaattaaa gcaagacatc
    59461 cgagatcaat taggccgtta cttatttgaa caaacaaaac gccgcccagt gatcttgcca
    59521 gtcattatgg aagctactca acgtcgaaat aaaaaataaa caaaaagagt aagatgaacc
    59581 tctctataga gtgctcgtct tgctcttttt ttgcgaaaaa caaataattt ttgaatacag
    59641 catccctcgc tttataataa acgatagaat aaagggagtg aatcaatgat gagtcaaaca
    59701 aaagtcgaac gttatttaga agaacaaaag atcagctatc aaccacttga ttttagtcat
    59761 gtggtagaag aatctttcac agaagaacta caaaaaagag ggattgatcc agctttgata
    59821 tgtaaaactc tcgtagtgaa aggaaacaaa actggggtga tcatcgcatt gattcctttg
    59881 aacgatcact tagattacaa aaaaacgaga caagtgacga aagatcgtaa agttggtttt
    59941 cctggaatgg attttgtatt aatgcataca ggatatcccc acggagcaaa cacccctatt
    60001 ggaataaaac tcgcacatcc tgattattgt tttttagcag acagttcttt aaaagaaaaa
    60061 gaagaagtaa ttgtgtcaag cggtgaaatc gggagaagcc ttcggataaa agtagccgat
    60121 ttagaaaaga tcgtccatcc tatttacgct gatttaatta gttaagggac gagaagagaa
    60181 gctaagcaga gcagagacct caaacttgag attatatctg cttggtactt ggtgcatcct
    60241 gtattatctg ttacaattgc tttgtacata ctgtactatc aatccaatga aaatatattt
    60301 agcagctata ttttttcatt gatgtgcaag tgatgaaatc gtttttaata aaaaagatac
    60361 agagaaaacg cgaggtgtaa aatatgaaaa ttacgtatca tggacattcc tgtttaacaa
    60421 ttgaaatgga aaatggccaa cgcgtgatga ttgacccgtt tattacagga aatccattga
    60481 cagacttaac tgcagaagaa gtaaagacag attggttgct gattactcat ggtcatagtg
    60541 accacatagg agatatgatt cctattgcaa aagaaaatca agccacaatt atcagtattg
    60601 ttgagattgc tgattatgcg aacagcagag gagtagattc attcggtatg aatatcggtg
    60661 gaaaacatgc gtttccattc ggcgtggtaa aattcgtaca tgcacagcat agttcaagct
    60721 atgaagtaga cggtatcgtt cagtatatgg gcgagcctag cggaattatt attcaagcag
    60781 aaggaaaaac gatttatcat gcaggggata ctgcttattt cagtgatcta ggattgcttg
    60841 cagaagaatt cgatattgat gtcgctttct tgccgattgg cgataactat acgatggggc
    60901 cagaagatgc agcaaaggct gcgcaaacaa ttcgtgccaa gaaagtagtt ccaatccatt
    60961 acaatacttt ccctgtcatt gaacaagatc ccgaatcttt tgtagagtta ctggaagata
    61021 gtgaaggaaa aataatgagc gtctcggaaa cgctggaagt gtaggcagaa aatggcgaca
    61081 aaacatgatt tgattttaga atatattgaa agtttacctg ttggcaatcg aatttctgta
    61141 agaagcatcg caaaagaatt gaatgtcagt gaaggaacag cgtaccgtgc aatcaaagat
    61201 gcagaaaatg tcggactagt ttcaacaatt caacgagtag ggaccatccg tatcgaacgc
    61261 aaattaaaaa aacatattga acgtttgaca tttggcgagg tcgtcaggat cattgaaggg
    61321 gacgttttag gcggggcggc aggattagac aaggtcttga ataaattcgt catcggcgcg
    61381 atgactaaac atgccatgga acgctatatt acgccaggat cactaatgat cgtcggcaac
    61441 cgggcggaag tccaaaaatt agcattggaa gatggcgccg cagtattgat cacaggagga
    61501 ttcaatacga ctgctgagat tgcaaagcta gctgatgatc ttgaaatgcc gatcttacgc
    61561 acctcttatg atacatttac agtcgcaacc atgatcaacc gagcgttaag cgaccaattg
    61621 atcaaaaaag atatcatgct tgtcagtgat atctatatgc cagctgaaaa gactcattat
    61681 ttgcagcaga cagatacgat ttctgattat cagcgtttgt cagaagaaac gcagcattcg
    61741 cgttttccag tcgtcaaccg ccatcatcgt ttgatgggga ttgttacggc aaaagatgtt
    61801 ttagggaaat cgccaaacca gatcatcgat cgggtgatga cgaaagaacc aatcagtgta
    61861 aagaaaacaa tgagtattgc ctctgttagc catcaaatga tctgggatgg gctagaagtc
    61921 atgccggtcg tttccgatga cttgacattg gaaggcttga ttacccggca agatgtaatg
    61981 aaagcgatgc agcttgtcca gcgacaaccg cagattgcag atacgatttc ggatcagata
    62041 tctggaaata tccatacgat tgatactgac cgtgaaggaa atcgtttgga aaaccctaag
    62101 ttcaaattcg tagtagcacc acaaatggtc aatggcgttg gaaccatttc ttttggcgta
    62161 cttagcgaaa tcatttctga tgtcgcacaa aaaacaatgg tgatgaacca aaaacgaaat
    62221 atcttgattg aacaagtgaa cctacattat ttgcggctga tccaattgga aagcgaatta
    62281 gacattcgtc cacgtgtatt ggaaatcggc agacgttccg ctaaactgga tatcgaagtg
    62341 tttattgaaa acaccatcgt ttccaaagca atcgttgttt gtcaagtaat ggaacgttct
    62401 tagaaagcag gagtgaaatg acagtacagc aagaaatagt agatacaata aaaaaatacg
    62461 ataaaatcat tatccacagg catttgcgcc ctgatcctga tgcattagga tcacaagtag
    62521 gattagccga actattacgg gaaagtttcc cagaaaaaga aatacgtcaa gtaggtgaaa
    62581 cgattgaagg gcttcgcttt ttagcagaaa tgcaagaagt tgaagatgtc ttctaccaag
    62641 gagcgctagt catcgtgact gatacagcta attcacccag aatcagtgat caaagatatc
    62701 agttggcaga aaaactgatc aaaatcgacc atcatcccaa tgatgaccct tatggagatc
    62761 ttgtgtgggt aaatacaaaa gcaagtagct gtagtgagat gattgcagaa tttgctttga
    62821 tgtttcctga atctttaaag atgaacgtta aggctgcacg gttactttat gcaggaatcg
    62881 tcggagatac agggcgcttt ttgtatccct ccacgacgtc ccgtacatta gagattgctt
    62941 cgatattgag aaattatcca tttgatgcaa gtgctttgaa cagagagatt gaacaaatgc
    63001 cgatgaaagt cgctaaactt tccggttatc tttatcaaaa tatccaggtg gatgaaaatg
    63061 gtgcaggaag aataatcctt ccgcaagaaa ttcttgataa atatggaatc gatgattcag
    63121 agacagcttc gatcgtttca cttcctggag tgattgacga agtgctggct tgggggattt
    63181 ttgttcagca gccttcaggg tattaccgcg tacgtttacg ttctaagggg ccgatcatca
    63241 atgaattggc taaacgccat catggaggag gccatccatt agcaagtggt gcgaatgcaa
    63301 aagatttagc agaagtagag gaaatctata aagagatcca agatatatgc caatcgtata
    63361 agagcagaaa aacagaatga tacttcgttc gaaaacgtgc cttttgtagt aaaaatcaca
    63421 aatgtatctt ttcaagatct tctttttccg ttacaatagt gctcactaaa gattttaaag
    63481 gagaaagaag atgacattac caaattgccc agaatgtggc tcagagtaca catatgaaga
    63541 tcgaggcttg ttgatttgcc cagaatgcgg gaacgaatgg aacaaagaag aacaagaaac
    63601 tgtcatagaa gacggattag tggtattaga tgcaaatgga aaccgtctgt tagagggcga
    63661 tagcgtgaca gtgattaaag acttgaaagt aaaaggtgcg tcaggtgcca ttaaacaagg
    63721 aacaaaagtc aagaacatcc gtttagtaga aggcgatcat aatatcgatt gcaaggtaga
    63781 aggatttgga ccgatgaaac tgaaatcgga atttgtgaaa aagaattaaa gaatattcaa
    63841 ggtcttttca cattcttttc ctactactaa tgataaaata aagtaaacga aaaggagtga
    63901 aacgaaaatg tatggttatg gaatgtatgg ttttttagat ccgacatttc ttttggtcat
    63961 tcttggatta gttatttccg gaattgcgtc agcgtacgtc aatagtactt tccgaaaata
    64021 cgatcaagtg agaagcaaaa acaatgtgac tggtacacaa gcagcacaat atatattaca
    64081 aagtcaacag ataaataatg tcggtgttca acaaattgcc ggagatctca cagataacta
    64141 caattcaggt aataagatgt tgagtttgtc tcaagcaact gcacagtcta cttcagttgc
    64201 tgcgataggt gtagcagctc atgaatgcgg acatgcggta caagatgctt cgggttatgt
    64261 cccattgaag attcgtgcag cacttgttcc agcagctaac tttggttcga tgatttcgtt
    64321 ccccttgatt atggtaggtg tattgcttag ctggaactct acgttaatca atatcgggat
    64381 tttcgctttt tctttagcat tacttttcca attggtgaca ttaccagtcg aatttaacgc
    64441 gtctcggcga gcaatccaaa ttttgagtga aggcggacta ctgacggaag aagaagtacc
    64501 gatggcaaga cacgtattat ttgcagcagc actgacttat gttgcagcag cgctttcgac
    64561 attcctgcag ctgttgcgct tgatcttatt gttcggcggc aaccgaagag attaaaataa
    64621 agaagttgtg aaatcagcgc tttgatctga gctaataccg agagttagct gattgaatac
    64681 catttatttg gaataagagg atgtgacaag aattttgtca catcctcttt gttttgttct
    64741 cataaccttt tttcatatac agttcatgaa ccgcttttat taacttgcca aataacactg
    64801 tattgtgcgt tgtatccaga tagaaagtct ccgtttcatc tgaaattttc tcatgctgat
    64861 ctgtaatgat caaatcagca tgttctgaat atgcggtaat tttaacagca tctttgcgaa
    64921 ataactgctg caaatggctt tgaagatgat aatttcctgt aaactgtttg gttatctgca
    64981 aatggatgag taactgctct ttcgtttctg tttgtagtaa gtgttggatc aattgactaa
    65041 ggtattgact gatttggttt ctttccccat tttttgaaaa aattgtatcg attttttctc
    65101 ttaatttctt ttcctgtata aaagaactag aatgattgga cgtggcttct ggaaagcaaa
    65161 gtgtagaaaa actactgctg gattcaccca acagttcgaa aaaaacaact gccaacacaa
    65221 gaaaataaaa ataaattggt attttatttt ctaaatggtt gagaggaaac tgttgagtta
    65281 agtaagtcat caattgaccg acttgtcgac aaataaggtt tttgcttgtg gaaaataaaa
    65341 aaccaatctc ctgtttttgt cgaaaagaga tttgatttgg aaaaaaacaa tagaaaaa
//
LOCUS       DANKEF010000011        62269 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000011 DANKEF010000000
VERSION     DANKEF010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 62269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.011"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(148..2301)
                     /locus_tag="PWZ48_000775"
     CDS             complement(148..2301)
                     /locus_tag="PWZ48_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HDL2260451.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2696..3544)
                     /locus_tag="PWZ48_000776"
     CDS             complement(2696..3544)
                     /locus_tag="PWZ48_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2260452.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3768..5708)
                     /gene="thrS"
                     /locus_tag="PWZ48_000777"
     CDS             complement(3768..5708)
                     /gene="thrS"
                     /locus_tag="PWZ48_000777"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDL2260453.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(6045..6224)
                     /locus_tag="PWZ48_000778"
     CDS             complement(6045..6224)
                     /locus_tag="PWZ48_000778"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HDL2260454.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6271..8094)
                     /gene="cadA"
                     /locus_tag="PWZ48_000779"
     CDS             complement(6271..8094)
                     /gene="cadA"
                     /locus_tag="PWZ48_000779"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2260455.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGVKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8372..10651
                     /locus_tag="PWZ48_000780"
     CDS             8372..10651
                     /locus_tag="PWZ48_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2260456.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETEIDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10744..11391)
                     /locus_tag="PWZ48_000781"
     CDS             complement(10744..11391)
                     /locus_tag="PWZ48_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260457.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNNVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11570..12289)
                     /locus_tag="PWZ48_000782"
     CDS             complement(11570..12289)
                     /locus_tag="PWZ48_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260458.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12420..13571)
                     /locus_tag="PWZ48_000783"
     CDS             complement(12420..13571)
                     /locus_tag="PWZ48_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HDL2260459.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKTTGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTYSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDVGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13788..14528)
                     /locus_tag="PWZ48_000784"
     CDS             complement(13788..14528)
                     /locus_tag="PWZ48_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2260460.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEIFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14532..14888)
                     /gene="rsfS"
                     /locus_tag="PWZ48_000785"
     CDS             complement(14532..14888)
                     /gene="rsfS"
                     /locus_tag="PWZ48_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016185667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDL2260461.1"
                     /translation="MIDSKEQLKIAVEAADSKRAEDIIALDIRNVSLLADYFMICSAN
                     SERQINAITEEIIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERSFYNLE
                     KLWSDAPLVDLSEWLD"
     gene            complement(14912..15505)
                     /gene="yqeK"
                     /locus_tag="PWZ48_000786"
     CDS             complement(14912..15505)
                     /gene="yqeK"
                     /locus_tag="PWZ48_000786"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HDL2260462.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15489..16139)
                     /locus_tag="PWZ48_000787"
     CDS             complement(15489..16139)
                     /locus_tag="PWZ48_000787"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDL2260463.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16149..16460)
                     /gene="yhbY"
                     /locus_tag="PWZ48_000788"
     CDS             complement(16149..16460)
                     /gene="yhbY"
                     /locus_tag="PWZ48_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDL2260464.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16476..17585)
                     /gene="yqeH"
                     /locus_tag="PWZ48_000789"
     CDS             complement(16476..17585)
                     /gene="yqeH"
                     /locus_tag="PWZ48_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HDL2260465.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNEIQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17582..18109)
                     /locus_tag="PWZ48_000790"
     CDS             complement(17582..18109)
                     /locus_tag="PWZ48_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HDL2260466.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18336..19121)
                     /locus_tag="PWZ48_000791"
     CDS             complement(18336..19121)
                     /locus_tag="PWZ48_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDL2260467.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRFDQHELLEKRYKRFRQY"
     gene            complement(19114..19983)
                     /locus_tag="PWZ48_000792"
     CDS             complement(19114..19983)
                     /locus_tag="PWZ48_000792"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HDL2260468.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHML
                     LELHTQKGWIDRG"
     gene            complement(19984..21363)
                     /locus_tag="PWZ48_000793"
     CDS             complement(19984..21363)
                     /locus_tag="PWZ48_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDL2260469.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPTRHIEVQILGDHF
                     GNVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21371..21790)
                     /gene="fabZ"
                     /locus_tag="PWZ48_000794"
     CDS             complement(21371..21790)
                     /gene="fabZ"
                     /locus_tag="PWZ48_000794"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2260470.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21815..22291)
                     /gene="accB"
                     /locus_tag="PWZ48_000795"
     CDS             complement(21815..22291)
                     /gene="accB"
                     /locus_tag="PWZ48_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDL2260471.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPETMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22294..23529)
                     /gene="fabF"
                     /locus_tag="PWZ48_000796"
     CDS             complement(22294..23529)
                     /gene="fabF"
                     /locus_tag="PWZ48_000796"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDL2260472.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIIGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23548..24285)
                     /gene="fabG"
                     /locus_tag="PWZ48_000797"
     CDS             complement(23548..24285)
                     /gene="fabG"
                     /locus_tag="PWZ48_000797"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HDL2260473.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24297..25214)
                     /gene="fabD"
                     /locus_tag="PWZ48_000798"
     CDS             complement(24297..25214)
                     /gene="fabD"
                     /locus_tag="PWZ48_000798"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDL2260474.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25321..25545)
                     /locus_tag="PWZ48_000799"
     CDS             complement(25321..25545)
                     /locus_tag="PWZ48_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2260475.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25598..26560)
                     /locus_tag="PWZ48_000800"
     CDS             complement(25598..26560)
                     /locus_tag="PWZ48_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HDL2260476.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26570..27010)
                     /locus_tag="PWZ48_000801"
     CDS             complement(26570..27010)
                     /locus_tag="PWZ48_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2260477.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27316..28282
                     /locus_tag="PWZ48_000802"
                     /pseudo
     CDS             27316..28282
                     /locus_tag="PWZ48_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
     gene            complement(28339..28569)
                     /locus_tag="PWZ48_000803"
     CDS             complement(28339..28569)
                     /locus_tag="PWZ48_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HDL2260478.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28655..29542)
                     /locus_tag="PWZ48_000804"
     CDS             complement(28655..29542)
                     /locus_tag="PWZ48_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDL2260479.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            complement(29631..30854)
                     /locus_tag="PWZ48_000805"
     CDS             complement(29631..30854)
                     /locus_tag="PWZ48_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2260480.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30871..31917)
                     /locus_tag="PWZ48_000806"
     CDS             complement(30871..31917)
                     /locus_tag="PWZ48_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2260481.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYSIRVPIQAISNGIDVEKYRPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(32081..33808)
                     /gene="ptsP"
                     /locus_tag="PWZ48_000807"
     CDS             complement(32081..33808)
                     /gene="ptsP"
                     /locus_tag="PWZ48_000807"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDL2260482.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDTMEEV
                     VELVHEYVK"
     gene            complement(33808..34074)
                     /locus_tag="PWZ48_000808"
     CDS             complement(33808..34074)
                     /locus_tag="PWZ48_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HDL2260483.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34553..36787
                     /locus_tag="PWZ48_000809"
     CDS             34553..36787
                     /locus_tag="PWZ48_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2260484.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36935..37255
                     /locus_tag="PWZ48_000810"
     CDS             36935..37255
                     /locus_tag="PWZ48_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HDL2260485.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37460..37684)
                     /locus_tag="PWZ48_000811"
     CDS             complement(37460..37684)
                     /locus_tag="PWZ48_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HDL2260486.1"
                     /translation="METEWQIRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37882..39462)
                     /locus_tag="PWZ48_000812"
     CDS             complement(37882..39462)
                     /locus_tag="PWZ48_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDL2260487.1"
                     /translation="MNNPELKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39863..41239)
                     /locus_tag="PWZ48_000813"
     CDS             complement(39863..41239)
                     /locus_tag="PWZ48_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2260488.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41367..42485)
                     /locus_tag="PWZ48_000814"
     CDS             complement(41367..42485)
                     /locus_tag="PWZ48_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2260489.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42627..42907
                     /locus_tag="PWZ48_000815"
                     /pseudo
     CDS             42627..42907
                     /locus_tag="PWZ48_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(42985..43518)
                     /locus_tag="PWZ48_000816"
     CDS             complement(42985..43518)
                     /locus_tag="PWZ48_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HDL2260490.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(43612..44790)
                     /gene="mutY"
                     /locus_tag="PWZ48_000817"
     CDS             complement(43612..44790)
                     /gene="mutY"
                     /locus_tag="PWZ48_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDL2260491.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(44783..45580)
                     /gene="recX"
                     /locus_tag="PWZ48_000818"
     CDS             complement(44783..45580)
                     /gene="recX"
                     /locus_tag="PWZ48_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDL2260492.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNRGKSQKELLRKIQQTLMTKGFSQEVIQQAMADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            45687..47069
                     /gene="rlmD"
                     /locus_tag="PWZ48_000819"
     CDS             45687..47069
                     /gene="rlmD"
                     /locus_tag="PWZ48_000819"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2260493.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMICEINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(47243..47821)
                     /locus_tag="PWZ48_000820"
     CDS             complement(47243..47821)
                     /locus_tag="PWZ48_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HDL2260494.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            48143..48613
                     /locus_tag="PWZ48_000821"
     CDS             48143..48613
                     /locus_tag="PWZ48_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HDL2260495.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGEYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(48721..49947)
                     /locus_tag="PWZ48_000822"
     CDS             complement(48721..49947)
                     /locus_tag="PWZ48_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2260496.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(50701..50901)
                     /locus_tag="PWZ48_000823"
     CDS             complement(50701..50901)
                     /locus_tag="PWZ48_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2260497.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(51035..51643)
                     /locus_tag="PWZ48_000824"
     CDS             complement(51035..51643)
                     /locus_tag="PWZ48_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HDL2260498.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNAAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(51728..52198)
                     /locus_tag="PWZ48_000825"
     CDS             complement(51728..52198)
                     /locus_tag="PWZ48_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HDL2260499.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(52246..53160)
                     /locus_tag="PWZ48_000826"
     CDS             complement(52246..53160)
                     /locus_tag="PWZ48_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2260500.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKNVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            53306..54109
                     /locus_tag="PWZ48_000827"
     CDS             53306..54109
                     /locus_tag="PWZ48_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HDL2260501.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            54287..55234
                     /locus_tag="PWZ48_000828"
     CDS             54287..55234
                     /locus_tag="PWZ48_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDL2260502.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(55302..55595)
                     /locus_tag="PWZ48_000829"
     CDS             complement(55302..55595)
                     /locus_tag="PWZ48_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HDL2260503.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(55623..56288)
                     /locus_tag="PWZ48_000830"
     CDS             complement(55623..56288)
                     /locus_tag="PWZ48_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HDL2260504.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(56428..57060)
                     /locus_tag="PWZ48_000831"
     CDS             complement(56428..57060)
                     /locus_tag="PWZ48_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2260505.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     ALRPDVILMDLVMEEMDGIESTKAILKDWPKAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNDPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(57067..58134)
                     /locus_tag="PWZ48_000832"
     CDS             complement(57067..58134)
                     /locus_tag="PWZ48_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2260506.1"
                     /translation="MMGRISKAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLMAISLGVGLIVFLLLSLIQKAKYGKIEEKLRALSSGNYESKLLHQP
                     IPSASDDLYIKDIDMEITKIKEKMIEVSSELQIMTSRPQYVDGQTKEEILKLERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWEVEDVKLTSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVETKVPLMKEA"
     gene            complement(58131..58862)
                     /locus_tag="PWZ48_000833"
     CDS             complement(58131..58862)
                     /locus_tag="PWZ48_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HDL2260507.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKYPRTKFQNFQLILGSLVIFFSLVNSPALWMMVVLAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMVQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAIISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKICSS
                     DYDENPRRLKIITNTLVGDVEVIRI"
     gene            complement(59030..59509)
                     /gene="greA"
                     /locus_tag="PWZ48_000834"
     CDS             complement(59030..59509)
                     /gene="greA"
                     /locus_tag="PWZ48_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDL2260508.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGD
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(59645..60820)
                     /gene="mltG"
                     /locus_tag="PWZ48_000835"
     CDS             complement(59645..60820)
                     /gene="mltG"
                     /locus_tag="PWZ48_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HDL2260509.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELYQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVQKYVNNS"
     gene            60985..62145
                     /locus_tag="PWZ48_000836"
     CDS             60985..62145
                     /locus_tag="PWZ48_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HDL2260510.1"
                     /translation="MSKGKHRKKQKISISFLVILLLSVSFFSTGIIIGGQLTKIPHSQ
                     TYKAEPTQLTSNLTPDEQRLASKIDQLLQKSDYIGSIYVRKNNRVLLEKGYGYANLRV
                     KESNSPSIYYQIGSIQKAMTALLILKQIQAGKLTFDTKLAEFYPQIPGSQQITIKNML
                     YMRSGLHRTASPDKPMSDSEIIQFALHHLKLTDYDTYHYEPLNYTLLTGILIKLTNQP
                     YETLLKKEIIRPLHLEHTDFYDNVKNSPQHAVSYQMSATDDYSKALIEPETDIRNELG
                     TGNISMSVYDLNKFFTSVLSGGIIPKELLFSLWQNNGDKHPYSGGVYSGNDYILAQGN
                     INRFHAVVAMKKDTKDAIVMESNIQSDKNIKLPATDLRNQIYELMEGVHLSN"
BASE COUNT        17964 a        13142 c        10496 g        20667 t
ORIGIN      
        1 ttttgtcaca accttttttg ccgagtaaac ggtgttcaaa agccagtttc tcggtatgaa
       61 gtcaaattta agcaaaaatg tgagaagcta ttttcacaaa atttttcttc atactcgaaa
      121 ctaagcggct ttttcacgac ctctttttta ttttgccatg aacatatcat aatacgcgtc
      181 cattatttct ttagcaatcg tcaagtttat gtggtcttgt tcgtctaata agttcggcaa
      241 gacaacactg atggcaattt ccggattgtc tgttggtccg tatgccacga tattactatt
      301 gactgtcgca atatctggat gaccttccgc aaccgtttct gcagtccctg tttttgcgga
      361 aaggtccatt ttcgcactgg ctaatggttt ggctgttgtg taaggatcgg taccatggac
      421 aacttggtgg aatccttcac ggaggatcgc catattttct gcagaaatat tgaccgtatt
      481 ttctactgtt gtgccaattt ctttcttcac ttcacccaag tttccatttt cgtcattgcc
      541 atagatcgct ttgactaggt gcggctgtac tcgtttcccg ccattagcga ctgttgaagc
      601 atattgagct aattggattg gtgtatatgt gtcaaactgt ccaaatgcca aatcggtaaa
      661 gttacctgga gcataccatt ctttgccatt atccgcattg aacttcttca tataatcaac
      721 agaacgtgag atcccaggag attcattcgg caaatcgatc ccagtcgttg taccaagacc
      781 aaactctttg aaagagtctc tcaattgctg ataagcttta gcttcttcat ctaaactagg
      841 tagtcccatt ccaggtgtat agtctagtcc taacatcttt aacgccactt tgatcatata
      901 cgtattagaa gacaattccg atgctttgac tgcatcaaga gcaacttgtc cgcttcgatt
      961 aaagacagag cttttttctg aagcaccttg cagacgaatc ggctcatcaa tcaaaacttg
     1021 atttccgctg atcgcgttac ttgcccagcc tgctgtcaaa gtaccagcct tgactacaga
     1081 ccctggatca aaggcgctag taatcgtgcc taaagcattc tcggtgattt ctttggaacc
     1141 ttgttcatga ctgaaaccag tcattgctag cacttctcct gtctgaggat tcatagccac
     1201 tgcataagca ccaggcgaat actgtgcttt gccgttgttg atcaatgttt gataattcct
     1261 tttcaaaatt tcttctactt ttgattggaa ttctgcattc atagataaca ttaaattaga
     1321 acctttttcc ccagagaaga tttctttttg attcgaaaca tttccttcat tatcaaggga
     1381 aacttcatat tgggttttgg ttccttgcag gacatcttca tattgttttt ccaaataact
     1441 ttgtcctaca cggtcattcc gagaataacc ttttgctagg tatttctctg cttcatctgc
     1501 tggaagtcct tgcttttcag tcgtcacact gccaagaata cttttcagtg aacttgctgc
     1561 attatattct cgtgtccagt ctgtgccagt cgaaactcct ggaagttcag aagcgcgttc
     1621 tgccacaaca gctaactctt gatcagtaac accagagttt ttgatataga cagtgtttaa
     1681 ttcatatgca ctattcatcc gtttaaagat ggtagcaatt tttaattgct cctcgtttaa
     1741 attaatctca tcagctgtga ctttattgac catcgcacta tattgttcgc tgttccctaa
     1801 ttgcttttct tttgccgaaa gtctttttgt ggcttctttc aaatgtttgg gatcagccag
     1861 ccaaaaatct tttttatctc tatctgtcag attttcatct accggaacat cgatcagcga
     1921 gttcagcttc gttgcgattt caagcaaatc tttagccgta acttgtgttc ctctcgtata
     1981 tgtgatggcc tgattcgctt tattcgtaac taaagctttt cctgtggagt cgtagatcat
     2041 tccgcgagga gtactttctt ggaccgtaat cgtagaagta gacttcactc gcgcctccat
     2101 ctcttcgccg ttcacgatct gcaaataacc caatctagca atcaacgcaa caaataacgt
     2161 aaatataatg aaaaaaagaa aatttaagcg aaaaggaaca tgggaccggc gaaacgtttt
     2221 gttcttaggt tcttgtgcac cttttcttat tttttctaac caagaagtat ttaatatttt
     2281 tttcataaaa tctttcatca tttcctatcc ttccgcctat ttacattcat gcttcattgt
     2341 acccaaaaac ttcttaataa acaagaagta tttaatattt ttttcataaa atctttcatc
     2401 atttcctatc cttccgccta tttacattca tgcttcattg tacccaaaaa cttcttaata
     2461 aaaaagaaaa aatgggcgga gcaagaaatt ctttacctaa tttcaagata aaatcaactt
     2521 tttgtttcac agcaatcact taaagttaca tatccaacta tttttttcat taaaaaataa
     2581 acttattttt gaaaattatg ttttcttaag tcgtgaaaca ctttgatgga tcatttattt
     2641 gtggaaaact acgttttgtt attctaaaaa acttatataa aaaatagtaa ggtttttatt
     2701 tcgaatccat aaaatattct tggattgctt ctcgtgtttt tttcactttt tcgctaagct
     2761 tttcgttttt agccatatag aaataaaaaa tgatatcgat cgtagcaaat tgggccaaaa
     2821 gtgaattagt tgccgcactt cgattcgtga tttcttttgg tctagatgtt tgtacgagta
     2881 catcggctat cttgctaagc gggttatttc caaattgcgt caacgcaatg atttcgatat
     2941 tcatgctttt cgccagctcg gctaatgcga ctacatctgc acttctacct gagtttgaaa
     3001 caagccaaag cactttcttt ttctcattgc tgacaagttg cgggagcaag acgtggatat
     3061 ctttttcaaa aatgattggc ttaccgacac gagaccattt ttgcagaata tcttctacta
     3121 ctaaagaaga ggcgccaacc ccatataaaa aaacagtctc tgccgattcc aaacactcta
     3181 cgactttttg gatcgtttct tcttctagaa tatctactgt ttcttttatc gctacttgtg
     3241 cattgttacc aagtttgttt ttgatagagt aaaaggactc atttgcttct atatcactaa
     3301 aaccttcatc gattccagtc tgtagttctg ttgaaatcgt gattttgaag tccgtcagac
     3361 tttgaaaacc gatttttttg gaaaaacgta caactgtcgg cgcactcaac ccggaagctt
     3421 ctgctaattc attagctgtc attgtaggga ccttctcaat attaccgata atgtagttgg
     3481 ccaacttttt ttctgctgaa gaataatctt ccatcatact ttcaatacga ctaattacgc
     3541 tcataagttc ctccaccctt ttaatgatag tctatgtaag tataccatat cagaccctta
     3601 ttcacggtcg tatactggat aaaaaacaaa aaaacaatcc gcccaacaga atatcttatc
     3661 ccatcaatcc cacagattct attgagcatc tgtgtctttg aggaatacct gttatctatt
     3721 gttcggattg ctttctatct actacttttt tacaggaaat ttacctatta gttttctctg
     3781 ctgtaatttt tgacttctgc ttccatagca tcaataaaga tattcaaatc ttctacagct
     3841 gtttctttac ttccataacg acgtacgttc actgttgcat cttccaattc cttgtctcca
     3901 acaactaatt ggtaagggat cttttgtgtt tgagaggcac gtatcttata gcccattttt
     3961 tcattacgat catcaacttc tacacgcaac cccttcattt gcaaacgttc tttgatttca
     4021 taagcataat cgccatgtgc atcaacagaa acagggatga ttgttgcttg tataggtgcc
     4081 aaccatgttg gaaaagcgcc tttgtatact tctgttaaat aagcgacaaa acgttccatt
     4141 gtagaaacaa ttccgcggtg gataactact ggacgatgcg tattttcccc gtcttctcca
     4201 acatatgtca aatcaaagcg ctctggcaat aagaagtcta attgtatagt tgataatgtt
     4261 tcttctgtac caagagctgt tttgacttga acatctagtt ttggtccgta aaaggctgcc
     4321 tcgccttctg cttcaaagta atctagttct aattcatcca tagcggcttt cagcattgct
     4381 tgggcttttt cccacattgc gtcatcatca aaatatttat ctgtattgtt tggatcgcga
     4441 tagctcaagc ggaaacgata atctgtaata ttgaagtcag catatactgc caccatcaat
     4501 tcaagcgtac gtttaaattc atctttgatt tgatcaggac gaacaaatgt atgaccatca
     4561 ttcaacgtca tttctcgtac acgttgtaaa cctgacaaag cacctgattt ttcatagcga
     4621 tgcatcattc ctaattcagc gatacgaatt ggtaattcac gataagaatg gatcgtgttt
     4681 ttgtagacca tcatgtgatg agggcagttc attggacgaa gaacaagcat ttcgccatct
     4741 cccatatcca ttggcgggaa catgtcttct tgataatgat cccagtgtcc agatgttttg
     4801 tacaactcaa catctcccat tattggtgta tatacatgtt ggtaacctag actaacttct
     4861 ttgtctacga tataacgttc gatcgtgcgt cggattgttg ccccttttgg aagccagaat
     4921 ggcaaaccag aacctacttc tggggaaacc atgaacaagt caagttcttt tcctagttta
     4981 cgatggtctc tttcttttgc ttcttcacgg agacggatat attctttcaa agctttcttg
     5041 tcaaaaaagg ctgtcccgta tacacgttgc atcatttgat tgtttgagtt tccacgccaa
     5101 taagcacctg ccactgataa taatttaaat acttgtatac ggccagtgga tggaacatgt
     5161 ggtccacgac acaaatcgat gaattcgcct tgttgataag cagtaatcac ttcttcttct
     5221 ggcaattcac taatcagttc tactttgtac ggatcacttg caaaaatttc taatgcttcc
     5281 tgttttgaaa gaacacggcg ttcgattggc agattttctt tgacgatttt catcatttct
     5341 gcttcgattg ccggcaaatc ttctgcagtt acttggtttt gtccattatc tgtatcgtag
     5401 taaaaccctg aatcgatagc tggtccaacg ccaaagtgga tatttggata gaggcgacgc
     5461 atggcttggg ccatcaaatg agctgctgaa tgacggacta agccaagtgc atcttcatgg
     5521 tctggtgtga tgatctcaat acttgcatct tcctcgattg gacgagtcaa atcaacgagt
     5581 tttccgttca ctttaccagc taaagctttt tttgctaagc tttttgaaat gctttcagca
     5641 attgccaatg ttgttgttcc agactcaaat tctttgactg caccatctgg aaaagtaatt
     5701 tttatcataa tagtttcctc cttgaaatgt tttgattgat cagatcatca agctgaaaaa
     5761 caaaaaaatc ctagcatctc ttgaaaagag atactaggac gaaaagttcg tggttccacc
     5821 taatgttcaa acgcaaatgc gttcctcaag cgttgtaacg gtccgaccgc ccttgcttca
     5881 acaagggttt cgaaagtggt catcctttgg ttttctcctg gaaaagtttc agcctagctt
     5941 ttcctctctt atcgaagact ctcaaattca gttggctttc tcatcaatca attatgtgtt
     6001 tatttaacca ctaataaata gaaaaatcaa gtgggaaact aaacttattt tttccattcc
     6061 ccatttacac cgtatgttcc ttttttcatc tcattgatga tgacatggat gtgttctttt
     6121 ggtgcctgcg tgtttttatg aacagcttcc gtgatatctt tcatcatatt ttctaattgt
     6181 tccggactgc gaccttctac taattcgaca tgtacgaatg gcataatgct ctctcctctt
     6241 tctgatcgtt cttttttcaa ctcgattcta tcatactatg tttgtcttgg gtactgaatc
     6301 aggaaaaatg gctggatctt tttttgctct caaggcactc aaaatagaga ccgtatccac
     6361 gacttcttgc aaaacagctc cgaataatgc ggggattagt cccgtacttg caattaacat
     6421 caaaaaaata caaataaaga tgccaatcag tacagactgt ttagctattc tcatcgtatc
     6481 tcttgaaatc actactgctt ttgctacacg caagagatca tctttcaaga tgaccgcatc
     6541 tgcactttca ctagcagctg tagaaccatg agcccccatt gcaataccga tatctgcagc
     6601 agcaagtgct ggagcgtcat tcacgccgtc tcctaccatc gcaacaggtc tttgatcttt
     6661 agggatactt tctaaaatgg tcagtttatc ttgaggcaag ctacgggcat gtacagaatc
     6721 taattggact ttttctgcaa tcgtttgagc aatggcagat ccatccccgg tgatcatcat
     6781 caaatttttc acatcaagtt ttcttagctc cttgattgtc tttgctgctt ctgggcgaat
     6841 ctgatccgaa aataggatcg ttcctgcaaa agatcgatca attgttacat agattgctgt
     6901 attggacgtt tctatttctt cagctgaaac aaaagaggca cgtccaatct ttacttcatg
     6961 attattgact actgcttgga ttccttgacc ggtcacttct ttcagttgac taacaggata
     7021 cagttggctg tttttcacgt tgttcactaa cgaacgagct aagatatggc tagattcctg
     7081 ttctgcacta gctgcataca gaagtagctc ttcttttttt actccattga ctggctgaat
     7141 cgcctctact tccaatatcc ctttggtaag cgttcctgtt ttatcaaaag caatcgtttt
     7201 tgtacgtgcg agcttttcaa tcgttgttcc agtttttaca acgatcccgt ttttacttga
     7261 acggctcatt cctgcaacta aagcaatggg cgcagctaga atcagtggac aaggtgatgc
     7321 cactaccagt acctcagcaa atcgtaaagg gtcttttgag aaccaccaag aaataccacc
     7381 gatcaaataa gcaatcaacg tgaaagggac tgcataacga tcagctaaac ggacaaatcg
     7441 ggcaggcttt tcttttgatt cttcgactag tttaataatc atttgatact ggctgtctgc
     7501 ggcacgtttt tcagcgatca tttggatcga tccgtctcca tttaaagacc ctgacataat
     7561 ctgatctcca ggtttcttgg tttccgggcg ggactcacct gtcaaagaag attcatcgac
     7621 cgaagattct cctttggtca ctcgaccatc tgcgggtaca agttcgccag gcttgactag
     7681 taactgatca tcaatattta tttcatcgat tactatatcc tgcaattttc cttctttgaa
     7741 tcgatgggca attcttgggg tgttatctaa caaagactgc agttctttac ccgctcgttt
     7801 attcgcgtaa tcctctagac tatctccgcc cgtaagcatg atcaatatca tcaaactagc
     7861 ccagtattct ttgacggcta atgttgcaag gattgcggta atcgctaata catctacgcc
     7921 atatcttcct gaacgtaacg tcttgatcat ttccaaaaac atcaagaagg cgagaatccc
     7981 tccagtaaat gcgacaatcg caaatgcaag ggccggctgg tgaaaaacaa attcacttaa
     8041 taaagctatc ccaccaataa gcaaagtaag taaaaatttt ttgaaatgtg tcattgtttt
     8101 ttctcctctt ttatgtaact tagagaggtt agtaataaat ttccagctgc ctctttcatc
     8161 ttaagcatag tccttgacta gcacttttta aaacaatagg ccttaattga aaacaactat
     8221 cactatcagc gtttccaaaa ttcatcagtt ctacaaacaa aaatattcag caaggattac
     8281 tttttcgatt catcagttaa aaagtacctt ttttcgaaaa actatgctat acttttaatt
     8341 cgttatttac ggcggaagga gcaaacatgc gatgaatgag aaacaactag aacaaacgca
     8401 cgtggatgaa acgatcgaac taatccagat cgaacaaaat atgctggata caaaacaaca
     8461 agaattgact caggaaatga atcattcgac aaaagacaca gctaatcaca agattcgagc
     8521 tggttccaat gaagcttttt atgaatctgc tgttgaatat cgccaacatg agcaggaatt
     8581 gcttttgaaa taccatactc ttgaagcaca gcaaaaacgc ctgcaaacgt tgtctgtcat
     8641 gaaagatagt ccttattttg cccgtatcga ctttaaagaa gaccgtgaaa cagaaacatt
     8701 atatctaggt atcgcttctt taagagatct ggcagaagaa acaatcgtta ttgactggcg
     8761 tgcaccaatt gccaatcttt attatgaagg tgagattggt cccgcatttt atgaaactga
     8821 aatagataga atagatgtcg atctgcagtt gaaacgacaa tttaaaatcc aagaaggtcg
     8881 tattttatct atggtagata cgtcggaagt aatcaatgat gactttttgc tagagatatt
     8941 ggatgaatca tcaagcaacc atatgaaaaa catcgtctct acgatacaaa aagcacaaaa
     9001 tgcaatcatt cgggacacaa caagtaaagt aatgctgatt gaaggaatcg ccggaagtgg
     9061 aaagacatcc gctttgcttc aacgtattgc ttttctgttg tatcataatc gcaagtggct
     9121 cgaggctgaa aacgttcttc tattttcacc caaccatttg ttttctgatt acatctcgac
     9181 tgtattgcct tcattaggtg aaagcggcgt gcctacacag acattcaaaa attatttggt
     9241 gcagctgctg ccagagtttt ctttactaga tgaacagcaa caagaaagtg gttttttgct
     9301 aggggaaaaa gatccgatac aagtgatgaa aagcggattg accttagtcg atcagattga
     9361 tcgctatatc caaggcatca caaactacgg tcccttgttc cgtgatatga aaataaatgg
     9421 ccgtacgatc ctatcaaagg aatcgattcg ccaatggtat aaagaaacaa atgaacaatt
     9481 gcctttacat catagactct ctctattaca aacgaagctc ctgaaaaaat taggcggtct
     9541 gcaaaaagac gaaacacgtc agcaatgggt caaagatctg gcggaagaac agctccaaga
     9601 actatacgca acagatccaa atcttgaata tacagaacaa aaggagcgtt cattacgtaa
     9661 aaaactggcc aaccaaatcg tacgtaaaag attccgtcga atccatcgag ggatcatgaa
     9721 ttatcaattt gtcaatataa cgaaacaata tctccacttt ctacaaactg tgccactttc
     9781 tcttctaaca cgacataaaa tcagcgagac cagatggcag gaacatttga caactgttcg
     9841 tacacacttg cgtcaaagag aattgcgtca agaggatgct gtactattct ttttactgat
     9901 gcgccgaaca tatccagtga cggttacaca caaagctcgc tatatcttta ttgatgaaat
     9961 gcaggatttc gcaccatcac aagtggcatt gctgcaagcc ttgttcccaa aagcaaattt
    10021 gactttctgt ggagatctaa atcaaaatgt ctttggaaat gaaacgatcg ttggatcatt
    10081 ggaagacctt ttcgtggacc gtgaagtcac tcgtttccaa ttaacgacaa gctatcgctc
    10141 cacaaaagaa atcactgatt ttgctaatca ttttattgtg aaagaacatc aagtagaaac
    10201 aacagcaaga aaaggagacc ttcctttgct catccaagga acaacagatg atatcaaatt
    10261 gaattggtta gcagatacca ttcgaacgac tcagggaaaa gcccggtatt ggcgtacagc
    10321 aatcatagct aaaacacaag aagaatgcga agctctttat gctcaactgc cagacgagtt
    10381 gaaacaacaa gtccagctga ttttaaatga gagtgacttt atgaaacgtt ccattatttt
    10441 acttccagcc tttttagcaa aaggtctgga gtttgaccga gttttcttgt gggatgtaga
    10501 tgcagaacat ttttcttctc agcaagacaa attgatcctt tacaccatgt gtacacgtgc
    10561 gatgcatgaa ttgacccttt ttacctccaa taatgaaagt cctttgttaa acaatatgga
    10621 tatttcatct tatgagaaaa aagtactgta agtaaaaaga caactgctat cattaatgtt
    10681 agcagttgtc tttttattta caataataat tgcagaaaaa gtaattcaat catttaaggt
    10741 catttattct atatataaat ccaaatctgt ttcaggatat tttaatttga caagcgaaat
    10801 aatcattttc agttcttcca tgttttctgg atattctggg tcaaatacct tgctatgacg
    10861 gccaatcccg ccattgtcga tcaatcggta aattcgattc cattcaatga attggtcaat
    10921 tgattggaaa ttgctgattg gatagccgcc acaatccaaa atctctgata cacgaaatgc
    10981 taggtatgca ctatcgatca cattattttt tttcgtgtct atcttctcat ttctttcgat
    11041 aagaattttt tcaggacgat gcttcaagct ccactctaat agtagaaaga aagcctccat
    11101 ccgatactct tcttcaaata accgaaaggc tgaatcagaa cgacgaaaaa gcagcggaat
    11161 cagataattt tgtctataaa tcagcattgg tgcgcggtcc accatacact ctaaataacg
    11221 gttcagatac cctgtaccga catagtttcc taatcgatag ccttcaacgc cgaatcttgc
    11281 gtatttttgc gctaattttg aacaatcagt tacgttcaca gctaactctt ctactttgcg
    11341 gtaaccgctt aagatacgtt caaaaggttc taaagtgatt tttgcttcca tattcttccc
    11401 cgtccatctt ccacatactt catatcattt cacatgatac ttatgctctc atcataccac
    11461 aggttgaatt agaaaagaaa ataaatcaca caaaaaagca agacaatctg ttagtgagat
    11521 tgtcttgctt ttcgaaaaag actgaatact tttcgttaaa actggttgct tagtcttcta
    11581 atgccacatt atggaacaca tgctgtacat cttctagatc ttccagtgca tcaatcattt
    11641 tttcaaatgt ttcttgatct tctggagata gtgttacctc gttttgaggg atcatttgga
    11701 tctcagcgat tgaaaaatca gtgattcctt tttcttttaa tgcttcttgt atactatgga
    11761 aatccgctac ttcaccgtaa acgatgattt gtccttcttc ttccattaca tcgcgaacat
    11821 cgatgtcttt ttccattagg tattcaagga tctcgtcagc gtcttctcct gcaaaagcaa
    11881 agattgctgt attgtcaaac atgtaagaaa ccgctccgga aacacccatg ttcccgccat
    11941 ttttcccaaa tgctgcccgt acgtctgctg ctgttcgatt cacattgttc gtcaatgtat
    12001 caacaatcac cattgagccg cttggtccaa atccttcata gcgcaattca gagtatgttt
    12061 catctcctgc accttttgct ttttcgattg ctcggtcaat aatatgtttt ggtacattat
    12121 aagttttagc gcgatcaatg acgaaacgta gtttttggtt tgcatgcgga tcaggatcac
    12181 ctgattttgc tgccgcgtag atttcaatcc caaattttgc ataaatccgg ctattattag
    12241 catctttagc agttttcttt gctacgatat ttgcccattt acgtcccata ttttcacttc
    12301 gctttctgat ttgatagttt cataacgcaa aaacgtcctg ttccattata cttgtgaatc
    12361 acgattctgt gaagcttttt ttctttcttc cttcattctt ctcattttta gtcaattatc
    12421 tatactcgga ttgggatttg cccgaaattc tgttccggaa tattgcctcc caattgatag
    12481 atttgatcac ttctaactaa cagttccgct tcttgtgcct ctttttttcc gattcgggaa
    12541 taaatagaaa tctctttttt ttcttttaag aatcttcttc caacatcggt aaatccaagt
    12601 agtcgaagat aatttttatt ttgttggaca ggaatctctt gctttttgat attcagcaag
    12661 acataacaag ataatcgttg gatccgtgtc catgtatatc tttttgtttt tacctttgac
    12721 acaaaatcct caaaactatc agcagttgca gctgctgttt tcatgcgcga ttctattcct
    12781 tcggtcatct gatagatatc tgtcagttcc agcaaagtag aagataacaa ccgataacgc
    12841 aaatacggcc aataagattc ccaagtctgc agcgaataag ttgaaagatc ttcttctgtc
    12901 tgaggcggca caagtccagc tattcttttt ccttcttcta acgcatggcg aatggctgtt
    12961 gcactggcta tcctctgatc tgtgatgatt ttttcatgat agcctgcact tttacgtcgg
    13021 atcggatgta attccatagg gtggacatac ttggcatttt cctttgcata acttaatccc
    13081 aagatatgat ttggcgaaga aaaatcaagc gtaatatcgg gatacagact gcgaaaaacc
    13141 tcagtcattt tttgcggata actatacgtt tgatcagaca gttcttgaaa tgcctcttct
    13201 atcctatctt gattctcttg gacaaagcgt ccgaaactgc tgtaatcaaa cgaagtctct
    13261 tcttctgttc caaaacacaa actgtcacag tccagtgttt gcaagatttt tactgcacct
    13321 ttagcaaaat agtcagctga ctgaaccgcc cattcgacgg gaagctccac tactaaatcc
    13381 acaccatttt gtaaagcttc attcgcgcga tgccatttat cgatgatagc cggttcgcct
    13441 cgttgcaaaa aattcccgct cattacagca atgacgacct ctgctcctgt tgtttttcgt
    13501 gccatttcca catgatagcg atgtccattg tgaaaaggat tgtactctac gataatcccg
    13561 cagcttttca tgcgcatcct ccttcatagt tatcgataaa aaagaaatag agacaagtaa
    13621 aaaaaggcaa aaatcggaaa aaatacccga actatataaa ctattctcgc aaacacaata
    13681 cttctgcgac gaaaagctgc actgcaagag caatgcctat atcgttcgga tatataattt
    13741 atgttgaaga tttattcttt tcttgttaac tcgagcaggc aaaaccgcta gtctttgtat
    13801 gctacaaaga accagcgctt tgatttctct gatgctgctt cgtctgtaaa atcagaaaat
    13861 gcttcgactt tcgaaaagcc gctgttttcc agcattctta aataactttc caatggatag
    13921 gtacgctctt gatgtagttc atcttcacga ataaatcgac cagacccttc ttgttcttca
    13981 acgaaaaagg taagaaaatg ttcgatgctg tgtttctcgc ttcctggata actatcccac
    14041 aaaaaagcaa aattatcccc ttgataatga tagctatatt ctggaaaaat ttcatctact
    14101 tgataggtgg agtgtacgtc aaataaaaat cggccatttt cttctaacgc ttgatagact
    14161 tcatcaaaca cttgctgtac ctcttgtcta tttttcatat agcacagcga atcagaaaaa
    14221 caggtaattg cctgatattg tccgatttct gataaatcga gcatattgcc ttccacaaat
    14281 tggatcgatg cgtcttcttc atatgctcgc tggctggcaa tcatcaacat ctcttctgaa
    14341 aggtctaacc ctgtcacttt atatccatct tttgcaaagg caactgctaa tgctccagta
    14401 ccgcatgcca gttccaaaat attttttgtg tcttcttgta aatgacgttt cgaaaaagct
    14461 agccattgct gataaagtgt ttcgtccatc acttcatcgt aaatgaaagc aaatgtttca
    14521 taagccatat gttaatccaa ccattcacta agatcgacta gcggagcatc tgaccatagt
    14581 ttttccagat tatagaaact tcgttctggt gcatggaaaa catgtacgat cacatctcca
    14641 agatcgatca atacccattt tccgccttct tttccttcaa tacgttttac ttcatatttg
    14701 ttttcttctt ctttatcaat gatttcttct gtaattgcgt tgatttgacg ttcgctattt
    14761 gctgaacaga tcatgaaata atctgccaac aaggaaacat ttcgaatgtc taaagcgata
    14821 atgtcttctg cccgttttga atctgctgct tcaaccgcaa tttttaattg ttctttacta
    14881 tctatgatga tttcctccta atatgagcag cttatttttt tgctacccag taattatatg
    14941 tttcgattgt ttttggataa atctgttgtt cttgcgcaag caaatgggcc aatgtatgtt
    15001 ttgtttcaaa tgctacagct tcgtctagat cagcccatgc aatggctcgt gcatcttgta
    15061 ccccaggaaa atctcgacct ggttcaatat agtctgctac ataaatgatt ttgtcaagga
    15121 gactcatctt agcagcccca gtcgtatgca cccggattgc atgaagaatt tctgcatcgg
    15181 tgattcctaa ttcccgctct acaaacgaag cgccaacaag accatgccag attgcattat
    15241 tataattcaa taactctgga tcaaaaccgt ctcttacgat taccattttg aactcctcat
    15301 ctggtcgttc ttttgcataa tcatgtgtca atgcagcaat actagctttt tcaggagaag
    15361 caccgtattt tttggcaaga gccacggctg tttcttctac tccaagcaca tgcttgaagc
    15421 gttgttcgct cattctcatc tgtatttttt gcatcaggtt ttctctagaa taagcagtat
    15481 attcattact atagtccata ttcatacaac ccttcatttt gaatataatc aattacttta
    15541 tctggaacaa ggtagcggat agaacagcct tctttgatct tttgacgtat tttcgtggaa
    15601 ctgatgtcga tctcaggtac atctacccaa attacaggat atggagtatc cgttgtgtat
    15661 cctggtcgac gtatacccac aaagttgacc atagaagtca gctcatcgat tttataccat
    15721 tttggcaaat attctaccat atccccaccg ataataaagt aataatctgt atccggattg
    15781 ttttgcgtca attccttcat agtatcatag gtgtaacttt tcccaccacg agctaattca
    15841 atcgtttcaa tctgcaaaaa aggattgtct tccacagcta attccaacat gttgagtcga
    15901 tgttttgcat caatcgtctg tttctcatca acatgaggtg gctgataaga aggcatcaaa
    15961 aatacgcggt caagacctaa attccggcca gcttgttcag ccatcactaa atgtgcaagg
    16021 tgaacgggat tgaatgttcc gcccaagatc cctacttgct ttttcttctc taagaattga
    16081 ggcatttctt caggaaataa ttgtgaacga acaaatgtct tagcttgcgt attcatcatg
    16141 gtcactcctt atattgcttt tacttctttt gatagccttt gatacttttc ttttgtggaa
    16201 ggtttgtata atacaataac acgtccaatg atttggacga tttcgcaatc tatttctttt
    16261 tctaatactt gagctgcttc ttctgcaatc tcatctgtat tttgaagtaa cgatactttg
    16321 atcagttctc ttttttctaa agcttcttca atttgaacga tcattgctga attgatccca
    16381 ccttttccga tttgaaaaat cggctgaaga tgatgcgctt tgctacgtaa aaaacgcttt
    16441 tgttttcctc ttaaactcat aattttcctt cttccttaaa tcaatgcttt gcgtttcaat
    16501 acatctacac cctttggcgc ccagcccgca attactactg gcgctgttac agtgatccag
    16561 cctagaccgg caaaaacgat gtctgttttt tctttcacag aaaactcaaa acgaacaagc
    16621 tccggaaatg cctcgacttc atcaggacga ggaggctgta ataatccacc tacatgtttt
    16681 tgataaaatt cgtctgcttt ttccagtttc gttcgatgaa tatttacatc atttgacaca
    16741 taagcaataa atgaagcacg attgccgctg ataaaatcaa atcttgcaag tccgcccaag
    16801 aaaagtgttt gcccttcatt cagctgataa actttaggtt tgatttcttt taacggtgca
    16861 atgatccgca aatctttttt acctaagtag tgcgccattt gatggcgatg gataattccc
    16921 ggtgtatcaa tcaaaaaatg cccgtcatct aaaggaatct cgattttatc caaggtcgtt
    16981 cctggaaact gcgaagttgt gattacatct tgtactcccg ctgtattttt gatgatttga
    17041 ttgataagcg tcgattttcc gacatttgtt actccgacga tatagacatc ccgtccatcg
    17101 cggtattctt cgattttttc taatagttcc gtcatctcat gagatttctt agcactagtt
    17161 aataatacat ctactggacg aattcctgct tcgtaagcac gttccttcat ccattgtgtt
    17221 aatttagttt ttttcaatga tttaggtaat atatccactt tgttcccaac taaaagtact
    17281 ggattgtcac cgacaaaacg gtgtagtcct gggattagtg aaccattgaa gtcaaaaata
    17341 tccaccacat tgacgatcaa tgcatctttc tgccctaatt cattcagtaa gcgcaaaaag
    17401 tcgtcatccg ttaatgagac atcttggatc tcattataat ggcgtaatct gaaacaacgc
    17461 tgacaataga cttccccgga agctaaacct ttttccaaag ccgtttgcgg cgtgtatcct
    17521 aattcaccgg gtttgtttgt ttgtataacg gcaccacagc cgatacattg taattcttct
    17581 gtcattcgat tccacctcgc cagcccatat cagggtgttt tgctaataga taggccataa
    17641 ttcttttttc ccaaaaacga ttaaattgtg tcttccaact atctgttgcg ataatgggtt
    17701 ttacaaggat gctacgtatc cctgcacgat tggctccacg tatatcagtc ataatctgat
    17761 cgcctaccat tactatctca tcatctgcta aatccaattg tttctttgct atgttgatcc
    17821 ctcttgctaa aggtttcatt gctcgtgcaa catacaaaag atcaaatttt tcaatcgctc
    17881 gcgcgacacg ttttgaatta ttgtttgaaa caacaacaac agggattcct gcatttttca
    17941 tttctaaaat ccaagtaagc aactcttctg ttccatctgg attattccat gcaatcaacg
    18001 tattgtctaa atcggtcaat actgctttga tgcctagatt ctttaattgg gcaggtgtaa
    18061 tctgatagat ggcttctacc atccatgttg gtttgtaatt tgaaaacatg ctgtctcctc
    18121 atctgcaaaa aatgaaggag cacataaagt actcctcaac cccgtattat actgcatatc
    18181 agcaaaaaaa actacctcta tactcgttcg tctttcagaa aaagcatttc tttacaaaat
    18241 gaccaaaaat aaagattgat cacagaaaaa tttagaaaca aaaaacaact aagcgagcaa
    18301 gaccattctt tactctttgc ttagttgtta aacacctaat attggcggaa cctcttgtat
    18361 cgtttttcca gtaattcatg ctggtcgaat cgaacaagtt ccgtcaatct ttcaatcaac
    18421 gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt gaacgtttca
    18481 ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat tttcatcaac
    18541 tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa tccttctgga
    18601 gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa acctagagca
    18661 agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac ctttaaatca
    18721 gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc tgctcctaca
    18781 ccgcaatatg cacctgctgt attgatcaat gtaatcaccg gtctattgaa cttttccgct
    18841 tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc aaaatttctg
    18901 cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac cggtttgcca
    18961 tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaacaaacg atcaccatgg
    19021 cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca tcgttcttta
    19081 gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc atcctttctg
    19141 tgtatgcaat tcaagaagca tatggatttt ctgcttcaat tccatgcggg gaacaatctg
    19201 atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg gaagttcctg
    19261 cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt ggggttctgc
    19321 tagaatgata tcgccttcca ttgcaaaact ggcagttact cctccggtcg tcgggtccgt
    19381 taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag cagagatttt
    19441 tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg aagctgtgaa
    19501 caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga tcttttctcc
    19561 gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa taatgaaggg
    19621 aatcccttga atggttgctt taccagtgag gactgcttca tgaagtcctg ttttatcttg
    19681 cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg ttacaagatc
    19741 cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct gccatgcacc
    19801 gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt ctttcgtata
    19861 aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag atggcgagtt
    19921 agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct taaacagagc
    19981 catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt cttcttgtaa
    20041 aaatgctgtg ctgtaatcac ctgctatgac tgcagggtga ctaatcaaat ccatctgaaa
    20101 ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct gcatcttcat
    20161 taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg aatcataata
    20221 tggcgggatc gtatatcccg ggtaaactgc actgtctacc cgcagtccca tcccacctgc
    20281 tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag caggattttc
    20341 tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt gacacacatt
    20401 caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc ctgtgaccat
    20461 ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat aaaaatcgcc
    20521 ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg ctttagctgc
    20581 tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta cagaaggaga
    20641 ttcttccagt actttttgat tattacgttg caaagaacaa tctcgttctc ctaaatgaat
    20701 gacattgcca aaatgatcac ctaaaatctg aacttctata tggcgagtag gataaatgat
    20761 tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct gagctgaaga
    20821 aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc cgccaccacc
    20881 tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca atgcttcttc
    20941 tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt cttgcatcaa
    21001 ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag gaccaataaa
    21061 tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata aaaaaccaaa
    21121 tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt cctgaacatt
    21181 cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg ctaactgtgt
    21241 atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc ctagttcgcg
    21301 gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata ctttagaaaa
    21361 catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt ttaccgtcta
    21421 ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc tcaacttcaa
    21481 ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc aatccgccga
    21541 aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct cctgcttgag
    21601 ccatcgcttc aacaattaat acccctggca taacaggttc ttctggaaaa tgtccttgaa
    21661 aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct tcaaccattt
    21721 cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct tttatttctt
    21781 gtatgttcat tgatttccct ctttcctgct ttgcttattt ttgctgaatc ctaaagagtg
    21841 gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc actccgtcgg
    21901 cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata acttctcctt
    21961 tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgccggt tgcagataaa
    22021 cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat tctttttctt
    22081 tcattgtttc tggagcagtt gttgcttgtt caggtatgac attgactggc tccgaaactg
    22141 gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt ttattcatat
    22201 acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga tcaaattgac
    22261 tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt tcatgcacag
    22321 aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa aacttgcagg
    22381 tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt ctacattgat
    22441 cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt cgatcccgcc
    22501 ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag tatctctgaa
    22561 attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg ctggtgtact
    22621 tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt cttccatcgc
    22681 aagcttcatt gctttagacg caccagaacc atctggagta ggagctgtca tatggtaagc
    22741 gtcacagttt gcaccgtatc caatgatttc tccatagatt ttggcaccgc gagcttgtgc
    22801 atgttctaaa gattcaagaa ctagaacacc tgctccttca cccatcacaa aaccatttcg
    22861 attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga gtgctgttaa
    22921 agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc cagcaagaat
    22981 gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat tcgttccgct
    23041 agcacaagct gttacgatag ctgtacaaat accttttgca cctacgcgca aagcaatatt
    23101 tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc gttttgcacc
    23161 tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga tcccagatcc
    23221 gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc tcatctccat
    23281 tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt tgctatcttt
    23341 tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg taatccctgt
    23401 ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt ttaagctatt
    23461 caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag taattaccac
    23521 tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc catcgacatt
    23581 gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt gtgcaatatc
    23641 ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct tgactttgtc
    23701 tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat tacatgtaat
    23761 gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc cagctttact
    23821 agctgcataa tttgcctgac caatatttcc aatcaaccca gatacactag agagattaat
    23881 gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca tattgaaggt
    23941 tccaatcaaa ttgatatcaa gacatttttt gaaatcttct gcatccattc gcatcacaag
    24001 tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa gcttttcttc
    24061 tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg aaactcctac
    24121 gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct ctccgcgacc
    24181 attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt tgccgattcc
    24241 tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt tcctccttat
    24301 agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac tggtattgtt
    24361 ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc gatgacttgt
    24421 tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg ttgcatgact
    24481 tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt tgtgttgctt
    24541 atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc cagttttttt
    24601 gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg gatcattctt
    24661 ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc ttcgccacca
    24721 atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta cttgctagct
    24781 tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac cattttcccg
    24841 cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa cgcaactgct
    24901 tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa actcagtcct
    24961 aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc gatgctgact
    25021 gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc tgtaaagcaa
    25081 agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg ctttacaacg
    25141 gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc actaaataag
    25201 aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt cttcctattc
    25261 aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta attttaatga
    25321 ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga ttccgtcttc
    25381 tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga ataaatccaa
    25441 gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt cagtttcttt
    25501 tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa tatattcctc
    25561 cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg agaatacttc
    25621 cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt cccaaggtga
    25681 tctttccact agaaactgct tcatccaata agatagggat gcttgcagca gatgtattcc
    25741 catatttatc catattggtc aggaactttt cttgcggcat ctttactttt cgggcaattt
    25801 tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt ttgtctttca
    25861 tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata tctctgccag
    25921 tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt ttattttgct
    25981 ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt tctttcagca
    26041 attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca aacaacacag
    26101 cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca atcactagtc
    26161 ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa acaaatcctg
    26221 agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc ccttgtactt
    26281 gtgctgctac agatggcgtc gtgtaatcgg gcgacattgt tgctactaaa ataaaatcca
    26341 aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa atgcataaat
    26401 cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt gacgaaatcc
    26461 attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc ttttcaggaa
    26521 ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct cacttatagt
    26581 cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt tcctccgtag
    26641 ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg acccgaaata
    26701 gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct tcactgcgga
    26761 tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag gtcccaacag
    26821 taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc ccgattgcct
    26881 cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc agctcagact
    26941 cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact gtttttaaat
    27001 ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt gatgttcaaa
    27061 ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac tatatgagct
    27121 ttttttaaga ttttactcat ttaaaacaac tgataaatca gcatttataa tttcttttat
    27181 tagaaaaatc atctatctca catattttga ttatcaaagt attttctgtg aacagaacta
    27241 tagaattcct taaaacttgt ccattatttc aaaatttgtt atagtctttt tattcaaagc
    27301 gtaaaaggag atcctatgtt aaaagaaaaa atgaaaactc tccccatgct gccgggtgtc
    27361 tatcttatga aagaccgtga tggtaaggtc atctatgttg gtaaagcaaa acggctcaaa
    27421 aatagagtaa gcagctattt tcatcagaat aaacagcatt caaaaaaagt attacgtatg
    27481 attcatcata taacagattt tgatttcgtc gtagtagaca cggaattaga tgctttattg
    27541 ctcgagtgtc aattgatcca acattaccgt cctttttata accgacaaat gaattatttc
    27601 agtaattata attatgtaca tattaccaat aagggatttg tattaacaga tactcctacg
    27661 gctcgaacat acgggccttt tcgtctctat aaaaaaatgc cgtctatttt gcgtataatg
    27721 gaagaaactt atcaaatgcc ttggttgtct gaaattagtt tgttagcttt gcgagcccag
    27781 cttcctgacc tacaggaaat gacttttgaa cagaaaaaaa aaggaactcc aagggctgtt
    27841 tcaaggccaa aataaaaagc tactaactta cttgaaaaaa agacaacaac atttcatcta
    27901 tcagttgaat ttcgaaaaag ccggtatgct tcaaaaagat atcgaattag ttacttattt
    27961 tattagacgg atccaggaac aaaagcattt tttaagaaca ccatcgctca ccttcagcat
    28021 gccattagca gcagatgaga gtcagaaaaa gcactacctc gtttgttatg gtcaacttgc
    28081 agaaacaatg attgcctctg ctgacgtttc tcctgacttc tattatgaaa agaaagaagc
    28141 tcatttatct ttgaaacgtc aattgtcaaa agaagaaatc gatcctgtac aaatcttgat
    28201 cagttaccgc aaaaaattag aaaaagaaca gtctgaaatt gaactgttaa acaaaaaaga
    28261 agctgaaaag caactaaatt agttgcttct cggcttcttt taggcatctt caacttcaaa
    28321 tgtcaattat cctaaaattt attggattaa gtcatagatt tccatcgcta cgatatcgat
    28381 attatcaaat tcgtattgct gatgagtatc tgtttcagtt aattcaaatg tatcggtcgc
    28441 tttatcataa gttactgtac acttctctac gccttctttt tcaaaacggc gaacttgcag
    28501 ttcattatct gctgactcga ccatagcctc cagtcgtttt atgattgcga ctaattgtga
    28561 atgtgtcatc ggaggtcctc cttttcttat ttctgttata ttgtatcaaa agcatagcct
    28621 acttgcattg attttctcta aaaaaactcg ttttttatga ttttttttct ggtatttttc
    28681 cttcaggcca ccaaagtttg atcaaagctt gggttccgtc attctcaaaa cctttatctg
    28741 ccaactcttc atatagtttc aatgcttggg tagttgctgg caatacaagg tccatttttt
    28801 ctgcttcttc taatgcaatc tttaaatctt tgataaaatg tttgacaaaa aaaccaggtg
    28861 tgtaatcttc ttttaaaata cgaggagaat aattgcttaa agaccagtta gctgcacttc
    28921 cacctgaaag cgtctcaatc actttcttta aatcaagtcc gtttttttgt gcatagacaa
    28981 gcatttccgt cattcctgtc atcgtacctg caatcatgat ctgattagcc atttttgtat
    29041 gctgaccttt cccagcagaa ccatgcaatg taaatgtcgt accaaattct ttgaataaag
    29101 gcagtacttg atcataaacg gcttcttctc caccgaccat gatcgtcagt gtaccattct
    29161 ttgctcctaa gtcaccaccg gataccggtg catctaacgc atctgcgcct ttttcttttg
    29221 ctgtttttgc aattttttct gccaggcttg gtgtacttgt agtcaagtca actaaaatat
    29281 gtcctgagat atctgcagaa aaaatccccg actgtccata gtagatttct tctacatctt
    29341 tggggtaacc taccattgta aagagaatat tactttgttc tgctaccgct tttggtgtat
    29401 ctgcccagat agctccttct gttactaaag gatcagcttt tgcttttgtc cgattataga
    29461 cagctacttc gtgtcctgct tctagtagat ggcgtgcgac agcacttccc atgacgcctg
    29521 ttccgataaa accaagtttc attttttcga cctcctaaaa atcaaaaatc cttgcttcta
    29581 tgctagtata cgctagaaac aaggattttt tcataaaaaa tgtttgaatt ttatctttta
    29641 aatgaattta attttatttt gattcgttca atcgagtctt cttcttcttt tcttcgcatc
    29701 tcttcttcat gataaacaac catgtcttga taaaattcga tcatagcttc gccgaaatgt
    29761 gtagatgaaa tatcataaag tttagcttct aaaacttttg gatctttagg aacttcactg
    29821 gaaatataat caataagtgc tggtgcaaag tcttcgtcgg tttcaaaagt tttccctaat
    29881 gaaggatgat caaacaatcg gttcaaataa gcatttcctt ccacgacaca aggtgttccg
    29941 gctgccatcg cttctgtata agtaagccct tgtgtttctg aagtagaagc actgacaaaa
    30001 taatctgccg ctttataata aagtgctaca tcattgttgt ccacttctcc tgtgaactgc
    30061 acaaattctt ctaactgcag ttctgagatc aattcctcta actcttctac ataaggtcct
    30121 tttcctacga taactaatct ggcgttagga taagcggaga tgacctgagg aaacccatta
    30181 atcaaggctt ggatattttt ttcataagaa atcctgctta aagaaagaat catcaaatgg
    30241 ttttcctgaa gacctagtga ctcccttaat ttgctgatat cctgctgagt gatatcagaa
    30301 cgtttgaatt tttcaatgtc gataccagta ggaatcacgc gaagcggtgc tattacacca
    30361 tagctacgta atgtatcaat aacacgttca cttggacaaa ctacacctgt cgtatgattt
    30421 gcaaataatc tagacaagta ttttacatgg gattgcctga tcacttttcc tttaccgata
    30481 taatgaaggt agtcttcata catcgtatgg taagtatgga tcagaggtat tttcaacttc
    30541 ttagctacca tcttcccaag tatccctgct ccgaattctg tatgggtatg aataagatct
    30601 agttctagtt ctttggcaac caaataagca taccacatgc ctctaacaac aactcttcga
    30661 tccttgaaag aaataaatgg tacacttggc attcgaatca catctttttc caacttatcc
    30721 gcattaggat cggtcgttgt aaaaatatag acttcatgcc catgtctttc taattcttgt
    30781 tttaatgttt tgatcgatgt agcaactccg ctgacttgcg gaaagtaggt atcggtaaaa
    30841 aagccgattt tcatgttttt cgcctccaaa ctagtccttg acctgattta ctttttttaa
    30901 tttgctagag actttttcat tcagtacact ttcgtataca tttttaagtt cttcaccgat
    30961 tttattgata cttcgctctt ttgctgtttg ataaccggct tgtgtcgtac tcggtagttc
    31021 ttgattggta atctgttgga tcagtcggat aaattcttcg tttgtcttcc ccatatagca
    31081 gttcttgcta tcttctaacc agccattata cactggaata tctcgaacaa gtacgttttg
    31141 tttgcttgct aaagcttcta aaacaacgat cccttctgtc tcttcataag aagggaaaaa
    31201 gaatagatct gcacctgaat acgcaccttc tatgatctct ccacgaatgt aaccaggaaa
    31261 caggacattt tcaggatgat cttcttttac aattttccgg atgttccgag gaatcgagta
    31321 catgggaaca tgaccaaacc aaataaatgt gtattcgggc atcttcttag caatttctac
    31381 gaaatcaatg attcctttac gttcgaaaaa caaaccgaca caaatgatta ctttttgatc
    31441 cggagacagt ttgaaatatt ctctgaactt ttgttctttc attggatcag ggcgatattt
    31501 ctctacgtcg atcccattgg aaatagcttg aatcggcaca cggatactat agctttccaa
    31561 caactgtttt gaataaggtg ttggcgtgat cagatagtcc gctttttggt acaatcctac
    31621 cagatatttt tttacgatag gagaaagctg gttcgatccg ataaaggaat ttcggaaatc
    31681 ttcctctgta gaatgcgcat gatagacaac tttttttcct tctcttctgg ctttattgac
    31741 catattatga ctattgattc cgtatgtatt gatgtgcagg atgtcataat cttctttttc
    31801 atctagtgta taagagatac cgacttccgt cagagcacgt ttttgatgat caagcgcacg
    31861 cccgattcca gattttgcaa gcatcttttc actttcaaaa tataataata ccttcacctt
    31921 acgtccccct tcacttatat agtataacat agcctacaaa ctgtcactca aacttaagcc
    31981 ctatttttat ctattcttac tgacaaaaac gttttcttaa aaaagaacaa aaaaactggc
    32041 gtagctcttt tacaagccac accagctttt gatacgttgt ttattttaca tattcatgaa
    32101 caagttcaac gacttcttcc atcgtatcgc attctttcaa tgcacgatca gcaagttcag
    32161 ccattttcgc tgtgtccaag cgtttcatta aactacgcgt tttaagaata gatgtcgcac
    32221 tcattgagaa ctcatctaga cccataccta ctagcaatgg aacagctgtt tgatctccag
    32281 ccatttctcc acacatacct gcccattttc cttcagcgtg tgctgcatca attacatttt
    32341 tgatcaaacg taaaattgat gggttatatg gttggtacaa gtaagaaacg cgttcgttca
    32401 tacggtctgc tgccattgtg tattgaatca aatcgttcgt acctacagag aagaaatcta
    32461 cttcttttgc aaacttgtct gctaagactg ctgctgctgg aatctcgatc atgatgccga
    32521 cttggatcgt atcagatact tccttgcctt cactgatcaa tttttgtttt tcttcttcaa
    32581 agatcgcttt tgctgcgcgg aattctttga gtgtagctac catagggaac atgatacgta
    32641 agttaccatg aacagatgca cgaagcaatg cacgcatttg tgtacggaac atttcgtcgc
    32701 cttgttcaga taaactgata cgcaatgcac gatatcctaa gaatggattc atttcatgag
    32761 gtaattgcag atatggtagt tctttatctc caccgatgtc cattgtacga acaactacag
    32821 gttttccttc cataccttct aatacagctt tgtaagcttc atattggtca tcttctgttg
    32881 ggaagtctgg agaatccata tacaggaatt cagtacggta cagaccaact gcttctccac
    32941 cattattatg gacaccaact agatcttttg gtgttccgat gtttgcagct aattcaaaat
    33001 gcttgccgtc agctgtgaca gtttctgcat tttttaattt ttcccattct gctttttgtg
    33061 ctgcgtaatc tgcacctgct ttttcaaatt cagctttttg ttcatctgta ggatcgatga
    33121 ttacatcgcc ttcgatcccg ttgacagcca aaatatcgcc ttctttgact ttagcagtaa
    33181 tttcttttgt accaacgatt gctggaattt ctaaagaacg tgccatgata gcagaatgtg
    33241 aagtacgtcc accaatgtct gttacaaacg cttttacaaa attgcgatct aattgtgctg
    33301 tatcacttgg agtcaagtca tgtgcaacaa cgaccacttc ttcgttgatc attgatggat
    33361 ttggtaaagt aacgcctaat aagtgcgcta aaatacgttt agcaacatca cgaatatctg
    33421 cagcacgctc ttgcatataa gcattatctt ccattgcttc gaacataccg atatacatat
    33481 ccgttacttc ttttagcgct gattcagcat ttacgctgtt gtctttaata ttttgcttga
    33541 tttgacctac catttctgga tctgaaagaa ccatcaaatg ggcatcaaat acttgcgctt
    33601 ctgcttcacc taagctttgc gctgcttttt cacgaatttg ctgcaattct tcagtagatt
    33661 tagctaatgc agcatccaaa cgagcttctt cagccgcagt atcttctact gttgccttac
    33721 tgaatgttaa atccggttga actagcaggt aagcttttgc aacggctaca ccgtcactag
    33781 cggcaatccc ttttagcatt tcaaccatta ttcagataag ccttctttct tcattgtttc
    33841 aacgattgct gccattgcat cagcttcgtc agcaccttct gcagtaatag taacatcaga
    33901 accttgacct acacctaaag acataacgcc catgattgat ttcaagttta ctgatttacc
    33961 tttatactct aagtttacat cagagttaaa tttgcttgct gtttgtacta atagtgtagc
    34021 tggacgagcg tggatcccag tttctgctac tacgtgaaat tctttctttt ccatattaat
    34081 cggtctcctt taagtgagtg ttgtttttat gtgagggttt tcttttttag taattcagag
    34141 aatcctaaca aatgccaccc tttcatctgt aaagattaac attttttttc gttagataca
    34201 acagttttca ttcaatttca gataaaagta ttcaaattat tccggattct ttttcatcct
    34261 tttcttcaca ttttataaca aaagtgttta ttttcttttt cttgtcgctt tctcttctcc
    34321 tgacgtttat cattctttcc tttgaccctc tttgttttca tatgaaacga attacttgct
    34381 tcccttttta tttttagtat aataagagtc aaaggtcagg aatggtcaat agagatttca
    34441 ccttttctac ctcataaatc tcttaaaaaa ctaggggtta ttcttttacc tcatgaaaaa
    34501 cgagatgtaa aatatggatg tacccatttt tgtgaaagga cgtgaatgat tcatgctttg
    34561 tcaaaactgc ggcaagaatg aggcaacgat tcacttgtat gccactgtca atggtcagcg
    34621 gacacaactc gattactgtc agagctgtta tcaaaaaatc aaaaatcaac aaaatggagg
    34681 tctgactaat atggcccaaa atgatccatt tggatttggc agtttggacg atcttttccg
    34741 ttccatgtct cgccaaatgc aacagcaagg aaattatgag caaacaccac caacacaatt
    34801 tggcggaaac aacaacttca atggtggtca gccacctcaa ggcaatacag aaggtttatt
    34861 gggagaatac ggaatcaata tcacagaaca agcacgacaa ggtgatatcg atcctgtgat
    34921 cggacgcgat gacgaaatca aacgagtcat tgaaatttta aatcgccgaa ccaagaacaa
    34981 tcctgtcttg attggagaac caggtgttgg taaaacagca gttgttgaag gtttagcaca
    35041 aaaaatcgta gatggtgatg taccgcaaaa attaatggac aaagaagtca ttcgtttaga
    35101 tgtcgtatct ttagtccaag gtactggtat acgaggacaa tttgaagaac gtatgcaaaa
    35161 attgattgaa gagatccgtc aagcagaaaa tgtgatccta ttcatagatg aagttcacga
    35221 aatcgtcggt gctggttctg ctggtgatgg aaatatggat gctggtaata ttttaaaacc
    35281 agctttagct cgaggagaat tgcaaatggt tggtgctact accttaaatg agtaccgcat
    35341 catcgaaaaa gacgctgctt tagaacgtcg tatgcaacct gtacgggtag atgaaccaac
    35401 tgtcgaagaa acgatcagta ttttgaaagg tttgcaaaaa cgttatgaag actatcatca
    35461 tgtaaaatat actgatgagg ccatcgaagc agccgcaact ttatccaatc gttacgtcca
    35521 agatcgcttc ttgccggata aagccatcga tttgctagat gaaacaggtt ctaagaaaaa
    35581 cttgacgatc caaatcgttg atcctaaaac aatcgagaaa aaactgcaag aagcagaaga
    35641 acaaaaagtt ttagcttctc gagaagaaga tttcgaaaaa gcagcttatt atcgcgatca
    35701 gatcaataaa cttcaaaaaa tgaaagaacg tcagctcact gaagaggaaa cacctgtgat
    35761 taccgaaaaa gatatggaaa aaatcgttga acaacggaca ggtattccag tgggtgaatt
    35821 aaaagaaaaa gaacagacac aactgaaaaa cttagcagat gacttgaaag ctcatgtcat
    35881 cggacaagat aacgctgtag atcgagtagc gaaagcgatt cgtcgaaacc gtgttggatt
    35941 aaacaaacag aatcgtccaa tcggctcctt cttatttgtt ggaccaactg gtgtaggtaa
    36001 aacagagctt gcgaaacaac ttgcctacga actattcggt tcacaagatt caatgattcg
    36061 tttcgatatg tctgaatata tggaaaaaca tagtgtctct aaattgattg gttctcctcc
    36121 aggttatgtc ggatacgaag aagctggtca attaacagaa aaagtacgga gaaatcctta
    36181 cagtcttgtc ttgttagatg aagtagaaaa agctcatcca gatgttttgc atatgttctt
    36241 gcaaatctta gatgatggtc gattgacaga tgcacaagga agaacggtca gctttaaaga
    36301 tacgatcatc attatgacaa gtaatgccgg aactggtaaa gtagaagcaa acgttggttt
    36361 tggtgcggca cgcgaaggtg ttactcgttc tgtattgaac caattaaaca actactttac
    36421 accagaattt ctgaaccgtt ttgacgggat catcgaattc agtgcattat caaaagaaaa
    36481 cttgatgaca atcgtcacac taatgctaga cgatgtcaac caaatgctgg cagcacagca
    36541 acttcacatc gaagttccta cgaatgtcaa agaaaaatta gttgacttag gctatgatcc
    36601 atctatgggt gctcgtcctc ttcgacgcac gatccaagaa caaatcgaag atggtatcgc
    36661 tgaattttat cttgatcacc cttctatcca tgagttgaaa gcaaaactgg ataaagatgg
    36721 aaaaatcatc gttacttcta aaccagaacg tttggctaaa gaatcagccg aagaaacggc
    36781 tgaataaatc cagttttata gcgtgagacg gtatttcttt gagaaatgcc gtctcattct
    36841 tttttcagaa aagtcaaaat actgttcatt ttataaaaaa gaaattataa taagagaaac
    36901 aagcaattaa aaatacataa aagaggtgag cgccatgaaa ttggtaaatg taacaaacag
    36961 ccattcccgt cttgtattaa accagcttga aaacactgat gcccatctag tcaaggtcta
    37021 cacagccgga aataccacta ttgtctacac tgaagcccct gaacataatg aaattctgct
    37081 gatcaatgac aaacgtaaaa tccagccgaa agaaatcgaa gatgctaaaa attatttctt
    37141 agataaaatc aaagacgccg tttaccacga ggatgaaatc aaagtaatcg aattggcagg
    37201 ccttgttgaa atctcgattc caaaacatga attaggcgct acattagccg tgtaaacaaa
    37261 ttttgaacat aataattaaa gagcgtggga caaaagtaaa gaacactttt gtctcacgct
    37321 ctaaacacgt ataaacggcg gaagcagaag caactccttc ggaaataagc taaaattcac
    37381 gaaaattgct tttcaaattt ttgtgaattc cagcttattt cttggagtta aacgcttctg
    37441 tctcagcctc tttttctttc tagctttttg atttttctct tttcttttgt acttttctct
    37501 tttcattttc agttctacgc gtgtctttat ttttaacttt ttcctcttta tggatgattg
    37561 atcgtacttt ttgtgcaatt tgctcgtttt tgtcccagtc ttttacatct aataatcgac
    37621 ataatcgtaa catctcttct tttttctgtt tttttctttc acgtatttgc cattctgttt
    37681 ccatgtgtca cactctcctt tctttaatca tatgtacgca aaataatttt tatatttttt
    37741 tgattagaca tttctatgat tttaatatag taaaaagagc cgtcacggaa aatccgtcac
    37801 ggctctaaac aaaatatgag aggctgtttt tgtttggcta ttcttattac ttgatgcagg
    37861 gcagcttgtg tcaagacttc tttacatcaa actttttaat tttacattag gatatttatc
    37921 cgcaaaccag cgttctgcaa attggttctc gaataagaac aatggttggt cgaaacggtc
    37981 acgagccaag atattgcgac ttgaactcat cttctcatcc aaatcttctg gatcgatcca
    38041 gcgagcgatt ttgtgtccca ttggagacat gattacttcg gcgttatact catttaacat
    38101 acgatattgg aatacttcga attgtagctg accaacagct ccaatgatat attcttccgt
    38161 tagatatgtt ttataaagct gaatcgctcc ttcttgaact aattgataga ttcctttgtg
    38221 gaaggatttt tgtttcatga cattttttgc agtcactttc ataaacagct ctggagtaaa
    38281 tgaagggagt tcttcataag caacttttag ttttccttcg aacaatgtat ccccaatctg
    38341 ataattgcct gtatcatata caccgatgat atcgcctgct actgcttttt cgacgttttc
    38401 tctcgaatct gccataaatt gtgtgacatt gcttaatttc agttttttat ttgtacgttc
    38461 caaccagaca tccattcctc gttcgaatgt tccagaacag attcggacaa aagcgattcg
    38521 gtcacgatgg gctggattca tatttgcttg gatcttgaat acgaaacctg aaaattcttc
    38581 ctcataagga cttaccatct gatcatcttg tgttttgtgt ccataaggag ctggtgccaa
    38641 ttcaacgaaa gtctctaaaa aagtctgtac accgaagtta gtcaatgcag aaccaaaaaa
    38701 tactggtgtc tgatcgccac gagcaatttt ttcttgatta aatgcatctc cagcctcttt
    38761 gattagttct acttcttcta agacttgttc atataaggaa ttttcagtta gaggatgatt
    38821 ttcgggaagc agtccatctt catccaattc tactaatcgt tcatcctgat aattttccgg
    38881 acgataaaac tctacacgat tatggtagat atcataaaga ccttctagcc ctttacccat
    38941 accgattggc caattcatcg gataagattc gatatctaat agctcttcta attcttctaa
    39001 caattccaat ggttcacgac catcacgatc tagtttgttg ataaaagtaa atatcgggat
    39061 gccacgtttt tttacaactt ggaataattt ctttgtctgt gcttcgatcc cttttgcact
    39121 atcgattacc atgaccgcac tatccacggc catcaaagta cgataagtat cttcagaaaa
    39181 atcttcatgt cctggtgtat ccaagatatt tactcgtttc ccttgataat caaactgcat
    39241 aacagaacta gtaacagaaa tcccacgttg tttctcgatt tccatccagt ctgatttggc
    39301 aaaattacct gtttttttgc cttttacggt cccggcttga cggatagccc caccaaacaa
    39361 tagtaattgt tctgtaatcg ttgttttccc agcatccgga tgggaaatga tcgcaaaagt
    39421 tctgcgattg tccacttgtt gctttaattc tggattattc ataacctaac tccatttctt
    39481 ccattcaatt atttatattt tctctaggca cagattgtcc gatctcttaa agaaagacaa
    39541 tttcatatag ggctgtgaca aaagccgtgt agcagtctct ttttccgagt aaacggcgtt
    39601 ctagagacag cctatcagca ataagcccac ccaagccaaa aatacgaaaa gctgtttttg
    39661 cttggctgct cttattactt aatgcaggat agctttttca caacctttgt caactactac
    39721 aactgaatca gtatagcatg gattcatcga atgaaaaagc caccaaacca taagagtcaa
    39781 atattttttt gtcagctgac taaaagaaaa gacgtgttgc ttgtttattc aagcaacacg
    39841 tctttcataa ccaagtttcc aattatcttc ttggctcgtc atcttcgaat tcttttctga
    39901 agtatcttcg ttcttcttca ggttccgctt ctgtttcttc cggatcatga aaaatgacac
    39961 ggattttaag gattcgagaa ccttccattt cttcagaaac cagcgtaata ttatcgacat
    40021 caaacgataa cttttcgcct tcatccggaa tcatgcctaa tgctgtaatc aaataaccag
    40081 ccatcgtatc tacatcactc atatgcagat cactgtcaaa agcttcattg aactcatcaa
    40141 tcagcatacg tccttggatg atatattcgt gttcatcgat tttttcataa agattctcaa
    40201 cttcatctga ttcatcatcg atttctccga cgatttcttc taaaaggtct tctagtgttg
    40261 ctaacccaac gacgccgcca tattcatcta ataaaatagc catctgattc tgcgtctttt
    40321 tcatttcata taacagatca tcaataaaga ccgtttcagg aacgaataaa ggctcctgca
    40381 tgattttctt gatatctaaa ttatcaaaac cgaatttatg ggcagctttt agtaaattct
    40441 ttgtatgaag gattccgact actttatctt tgtcttcgtt ataaacaggg attcttgaat
    40501 agttttcgga taaaacttca ttcacatttt cttccacagt gtcgttgata tcaatcataa
    40561 atgcatccgt tcgtgggacc atgacttctc gggcaacttt cgtatcaaga gaaaaaaccc
    40621 cttggagcat ttctaattct tcattattca atacgccttc gttttcaagc atataacgca
    40681 tttcatctct ggtcattttt gaatcttcat catcaaaagt catcggcgtg atacgcgcca
    40741 ataaacttgt ggaagcagaa agcagccaga cgaagggctt agctactaca ccaatcaagc
    40801 gtatcagccc tgaagtgaac tttgcgactt cttctgattt atttaaagca atacgtttag
    40861 ggtacaattc tccaaaaaca atcgatacat aggtcaaaat agccagcaca atgatatttg
    40921 caatattttt tgccgctgtg ccgccaccta ataccggagc taaccgttcc gataaagtat
    40981 tagctagtga agcaccagac aaaatgttga ccaaagtgat tcctacttgg attgtcgata
    41041 ggaaattatt tggattctgc aatattttta aaagcttttg agatttcaca tctccttcat
    41101 cagctttttg ctcgattcga ttcttattga tcgaaacaac agcaatttcg gaagctgcta
    41161 aaaaagcatt gatcaatgtc aaaacgatca ataataaaat ctgcgctaac agcgactgac
    41221 tctcagggtc agcattcata atcgcattct ctccttataa aattaatttt tagtttacga
    41281 tagaaaaaaa cgtggcacta tagccacgtt aaaatggtat cacaaagcaa ttttctttgc
    41341 aataaaaaca agtgtactaa aactacctac gaggtcgtag caattttctt cttgttttca
    41401 ttttgcttgt cttctttgac gattttgatt atataggaaa tgatcgtctt gcagattgcg
    41461 taaaaaggaa cacctaagaa gataccaagc aatccggcaa tatttccagc tactagaaga
    41521 acgataatga tcgttaatgg atggattttc agtgatttcc caattacatt aggataaatg
    41581 atattcccat ccaactgttg gacgattagc acaaccacac aacaaagtaa tgcttttacc
    41641 ggatcattga agactgttac tagaaatgca ggtgctagtc ctagataagg tcctaaatat
    41701 ggaattaggt ttgtcaatcc cgcaatgacc ccaaataaga aagcataatc cacaccaatt
    41761 accatatatc ctaaaaatgt gaaggtacca acgaataaac attcgattgc ttgtccgcta
    41821 atgtaattag ccaatgtttt attcagttgc cccaataact ccacaatttg gtttcttctt
    41881 ttttctggga aaaactgttg gatgtttggc actagttttt ctccgtcttt caacatatag
    41941 aacaagatga atggtgctgt aatgattact acggttgccg aagcgatcgt tccaacgatc
    42001 gaacctaaac tattggatac tccactaagg aattgctgaa tgattgtccc gtaagaaatg
    42061 tctaattgct caaaatattt attgatatct acttccttga acaaaggcaa ttcggctacc
    42121 tcttttaacc agctttctac ctgtttgatg aaatcaggca tattagatgc caatgaggaa
    42181 atttgctgta ctagacttgg gatgacacta agtaaaatcc aaataattgc tgcaactaaa
    42241 aggagcagca caattgcgac agcccatatt cttttgattt ttgctttcat caacagcttg
    42301 acaataggat tcaataaata gtaaagaaag cctgcaatca gtacaggtgc aaataacgtg
    42361 gaaaaaaatg tcccgatcgg ttcgaaaata aagtttattt ctgatgaaat catgatcaat
    42421 gcagctatga tcaataattc aagcgaccaa aacattagtt tagactgtct aagtttttca
    42481 aacaatagat ttcctccttt ctttcttact agtatatatt atattcgtta aaaattcttt
    42541 tattcttttt ttatttttga aaacttgcca tactttttat ttgttttatt ttccagtata
    42601 ctatacgaaa ggaaggtggc tagaccatga aagttgtaca aacaaaagat acaatgagca
    42661 atatttactt agatgctgta cggatcaggc atcaagtttt tgtagtcgaa cagggtatgc
    42721 ctttgtctcg agaaatagac aaagatgaag cccattgtat ccacttcgtc ctgtactcag
    42781 ataaaaaaga accgcaagga actgtacgtc ttttgccttt agaaaacgga aaaatgaaat
    42841 tacagcggat ggcgatcctt ttgaagatgc tggcatgcct catatttata tgaaaaagac
    42901 tttataaaaa tttatttcga aaaaagcaaa aaacagcaag cttttgagaa aaatctctca
    42961 ctggcttact gttctttttt gtgtttactt acgcgacaat tgcttgtaac ggttatacca
    43021 aatatcaata tagccttccg agaacgggcc atctccatta ttgatccagt caaccagaat
    43081 cttcacattt tcttttagga tgaagtcaat atcgttcgga taattcatca ttttgctgtg
    43141 catctcatat tcatcgacat ccaatagtcg tttctcgccg tcaggaaata ccttgatatc
    43201 caagtcatag tcgatatatt tcaaggcctc atcgtccagc aaaaaaggtg aagctagatt
    43261 acaataatag gaaaccccct tttctctgat cattgctatt acattgaacc agtatttttt
    43321 atgaaaataa acaatcgctg gttcgcgcgt tacccatcgg cgtccatcag attcagtgac
    43381 taatgtgtga tcattcacgc cgatcataga gtgttcgctt gtttttaata ccatggtatc
    43441 tcgccacgtt cgatgcaatt tgccgtcgtg cttgtaactt tggatcgtca caaactctcc
    43501 ttcttttgga actcccatag caacccttcc ttaagcagca ttttgtgctt tgtgtttggg
    43561 tgttcattac accgacatca taattattat atcaataaaa atgagctttg cctagaaagg
    43621 attcctattg tttcgatttt tctctaactg atctaccagt ttcatttgga ccttaggaaa
    43681 aacgaccgaa tcaaatgcat caggcttcaa ccacttcgaa gttttgttct cggtaaattc
    43741 ttgttctgct ccctcggttg cccgtccgta aaaaaggaga acatgccatt tcagatgact
    43801 aaaaatatga gtaacttctc ctaaatgtcg tgtctgccaa acaaagagtt cctgcttttc
    43861 aaatgggaga ttttgctctt cttctgctac taggtcatca aacagatcga tttctggctt
    43921 tgtttcccat tcttttttca atcgctcata ctcctcttgt gtcacttcca tcattggaaa
    43981 tgtccacata ttcgccagca atttttggct atctctttct tcaaaataat acgctccaga
    44041 atgattttga agagccgcac tgatataata aacgtctttt ggctttgctt tttttgtttt
    44101 tacaggaaaa ctcgtttgga ttcctcgttt gttagctaaa caaaaggctt ggatcggaca
    44161 agtctcacac tttggagaag ttggtgtaca gatagctgaa cctagatcca tcattgcttg
    44221 gttgaattca cctggatgct tttcatcaat gattttccgc atcgcttcat caaagatttt
    44281 cctgctagaa gcttttgcga tgtctgcttc aatgcaaaac aatctgctga ctactcgcat
    44341 cacgttgccg tcaaccgcgg gttctggaag tccaaatgcg atacttgcaa tcgcgcctgt
    44401 cgtatatggt cctattccct tcaatgagct aatttcttca ggcgtttgcg gcatttcccc
    44461 atcaaattct gacatgatct gtttagcagc agcttggatg ttccgggctc ttgagtaata
    44521 accaaggccc tcccatgctt ttaaaagctt ttcttctgga gcatttgcta gctcttcaat
    44581 tgtaggaaac cattccatga aacgataaaa ataatcgatc actgtgtcta ctctggtttg
    44641 ctgaagcatg atctcagaga tccagatacg ataaggatca cgattatagc gccaaggcag
    44701 gtttcttttt tcttgttcat accattgaat gaactgatct tgaaattctt ttgtttcttt
    44761 atctgtccat ttttcccatt ctttattcac tttcctcttc cttttccgta atgaatcgct
    44821 ggatcatatc caaatcaaat cctttttgat ataagctttg ctttactttt agatttcttt
    44881 ttttaggttc aaaccttaaa tttcttcgcc atagtttatc tccttgtttg actaaataat
    44941 catattctat ttcttgatct gcttcttgcg gaagatcagc catcgcttgt tggatcactt
    45001 cttgagaaaa acctttcgtc atcaatgttt gttggatttt tctcaataat tctttttggc
    45061 tttttccgcg attttttcca aacgcttttt cggctgtctg atgtgcattt tgaacttggt
    45121 cttcaaatgt atattggctg agcgcttgtt caatgatttc aggtttcaaa cctttttgct
    45181 gcatctgctg cgctaatttc tttggtcctt tgtcacttaa gcgcatattc gtgcgcacat
    45241 aactttcccc atagaccaaa tcatcgacaa ggtccaattc ttttaagcgg gctacgattt
    45301 tatttcgatc ttcttgaagg atttcctttt cttttaagta tgttcggatt tctttttccg
    45361 atcgcaactg ataacttaag taattcattg ctaggtgaaa accgaaatca gctccgctac
    45421 tcttcttgat ctcttggata gtttcttcgt ctaattcccg tccttttaat aatcgatagc
    45481 gtactaatac atcttcagat acttgtaaag cttctccttc agataaatct acgcgataga
    45541 aagggccttt gcctttactg attctcgtaa cggttaacat aaacatcctc ctcgaacata
    45601 tattctctat cataccacag aaaatccccg taatcaggta ttatatgtta gaataaacca
    45661 cagaaaaaag aaaaagggag atacttatgg cagaaacaat tgtcaaaact ggacaaacgg
    45721 ttcctttgaa aatcaagcgt ttaggcataa atggtgaagg aatcggttat ttcaaacggt
    45781 tgatcatatt tgttccatat gcactaccaa aagaagaagt actagtgaaa atcacgaaag
    45841 caactcctcg ctatgcagaa ggagaattaa taaaagtcaa aaaaacaagc aaagatcgcg
    45901 tagtggcacc atgtcctgtt tactatgaat gcggtggctg tcaattacaa cacttagctt
    45961 atcaagctca attggatttt aaaaaggatc tgcttttgca agcattggaa aaattcaaac
    46021 cagcaggctt tcgatcttat caacttttgc cgacgatcgg gatggatgag ccttggcatt
    46081 atagaaacaa agcacaattt cagttacgaa aaaataagca gactcaaaaa attgaagctg
    46141 gtttatacga agcaaactct catcagttag tccctataac agactgcttg gttcaagaac
    46201 ctcgaacaca aaaagtaatg aatactgttg ttcaactatt gaataaattt gatgcaccca
    46261 tctatgatga acggacaaac agtggaatct tccgcacaat catggttaga atcggtgtta
    46321 aaactgggga gttacaagtt gtttttatta ctcgcagtaa aaaattcccg caaaaaaacg
    46381 caatgatctg tgagatcaat gaacgactgc ccgaagttgt ttcaatcatg caaaatgtcc
    46441 aggcacaaaa aacgtctctc gtgatggggg atgaaacgat ccacttatgg ggaaaagaag
    46501 ccattgaaga acaaatcaat gaagtgactt tcgatctttc tcctcgtgcc ttctttcaac
    46561 tcaatcctca gcagaccgaa ttgctttatg gcgaaggaat caaagcatta gatgtccagc
    46621 ctaatgaaac gatcgtggat gcttattgcg gagttgggac gatcggctta agtgtagcaa
    46681 aacaagccaa agaagtacgg ggaatggata ccgttcctca agcaatcgct gatgcaaaaa
    46741 aaaatgcgga aagattagga tatcacaaca ctcgatatga agtcgggaca gcagagcaag
    46801 ttctgcctaa atggttgaat gaaggttttc agcctgatgg tatcatcgta gatccaccac
    46861 gtacgggttt ggataacgca ttgatccaat cattattgaa ataccctcca aaaaaacttg
    46921 tttatatctc ctgtaatgtt tctacacttg ccagagatct ggttaattta agtaaaaagt
    46981 tcaagattga atatttacag tctgtcgaca tgtttcctca gactgcacgt tgtgaagttg
    47041 tcgtgaagat gacgagactc gctgactaaa tgaaagaggt tgtgaaaaag tctgccctgt
    47101 atcaagtaat aagaacagcc aaacaaaaat agctcttcat gttttatgaa taccgtactt
    47161 gtgaccgaag agttaacagg aggacactat ttatttaaaa aaagggttgt gacatagttt
    47221 ttgtcacagc cctttttgat tattattgga tattcacgtc tatcacttga taaaatgcat
    47281 ttgtcgtatc gctgatattc catactgcta aaatcacatt atatcctttt cgatcctgcg
    47341 gaagtgtgat cgtttggttg actgtttttg gtggaacagt tgctttgtcg tctatttttg
    47401 tgatcaattc aaaatcacta aatttcaaag gttgattagg attccaagtt ggtttggtaa
    47461 tgtagtaatc ccaagtactt gtccgatgtt gtgctgttaa attccaagta atatttaatg
    47521 ttcctggatt aaccatgttt ttatgccatc tgttagatgt ctgttcatct aaaggctcaa
    47581 agccgctgac tcctgcacta gcaatttttc catcaatgaa tgtatttttg gtagcttcga
    47641 tactttgcgg ttcccattgt gctcttccga cattttgatt taagtttcct gctgctgatg
    47701 ttcctaggta tgcacggctt cctggagaag taatataacc atgcgcaaaa cctggaatgc
    47761 taaaaccgaa caaccctaaa ctaactaacc caataaacaa taacgtacta atctttttca
    47821 tgtagaaacc tccttcattt gataaccaca ttgtaaacgc tttattgtat taacacaaac
    47881 attatgcttt tataatttta tgttgaagta aaaaaggtga aaaaagtaag atgcagatta
    47941 taacagaagt agacgagaaa agtacaaata gaaaacatga gaaccaaatc atatgatctt
    48001 tttcaaaaac atcattcata taaacaaatg ttcaactttt taataacaac aatcatcaat
    48061 ttttttctta cttcacaaac taaatacagg tcttgctgtg agccttcatg gtaaactact
    48121 attatagaaa ggagttagta aaatgaagat gataaaagat attcgttttt gggtttgtgt
    48181 tataggaatt gtcatattag gctttttatc agggttgctt agcggaaatc ccggagaata
    48241 ttactattct ttgcaacttc caccatttgc accacctagc tggatatttg ggccgatgtg
    48301 gaccttactg tatatattga tgggtatttc tctttatttg ttacttaatc acaacaacaa
    48361 aaaacagcga aacaatcttg tcggtctttt tgtgattcag ttcattttta attttatatg
    48421 gtctgctcta ttttttaatt taagaaatat tttcattgca gcaatcgata ttacactttt
    48481 agttattttc ttgtcagtgc ttatgtatca gttgtggcta catcatcgcc tggcaatgtg
    48541 gctcatgatt ccttattatt tatgggtact attcgcaact ctattgaatt attcaattta
    48601 ttttttaaac taagcgctaa aaagagacgt cctgtaattt aacacataga aaaagtgcca
    48661 cctaagttag attttttgtc taatttaggt ggcactcttt attaaccaaa ctattttttt
    48721 ctagtaccca cttgttgttc catactcatt attctggtca taaccataac cattttgttc
    48781 ctgtccagcg taaggatcat aactttgagt atcttgactt ggtgtatagt actgacttgt
    48841 atcttctgtt tgctgataat aggtattcgg atcttggtac tgatcatatg tgttttctga
    48901 ataggaagaa taatcggtgt acccttcact attcgatcca tcaccgtttg tcgaatcgcc
    48961 atcttccgaa tcatcaaaga acatcattga agcattctca tttttcaaat taggtaatgt
    49021 atcgcttgtt tttatattta attgtttctt cagttcattt tggatcgaaa gaagatcatt
    49081 ttttcctaaa atttgatagt agatatcatt gatcatttgt ccttcgcctt gcaactggtc
    49141 ttgtttgatt gtttcaaacg caggggtgta gttcgtcgct acatcaagca tgtcatccca
    49201 atctaagtcc gtcgtcacat tgttctcaac tgcttttaaa atttttctat aattgcttac
    49261 gccatctaaa cttaaaactt tattgacgat tttcgtcact acttcccgct gacgacgttg
    49321 tcgaccgata tcgccttctg gatcttcgtg tcgcattcga gaataggcta aacctttttc
    49381 tccattcaat gtttgttcac ctactggtac atggacacca tccaaagtaa agtcaatttt
    49441 attattcact ttgatgccac cgaccgcatt gatcaaatct tccatgccat ccaaattgat
    49501 cgttacataa tgatcaatag gaatatcgag cagttgctca attgaatcca tggccatttc
    49561 gacaccacca aatgcatagg catgatttaa tttatctacc gttccataac ctacgatatt
    49621 cgtataaata tctcggtcca aactgatgat cgttgatttt ttttgtttcg gattaactgt
    49681 tactaccatc atcgtatcag aacgtccttg ttccgttctt cctaatccac cggtatctaa
    49741 acctaatagc aaaaccgaaa aaggttcttt gtcatcaatg cttactctct cagaacgttt
    49801 ggaggatatt cgattagagt ttttagaaat tttgtttact gtttggctcg cttctcccat
    49861 aaatctgatt ccgtatgctg ataatccggc aatcagcaaa acaaataccc ctaacaaggt
    49921 taatagaact ttttgccatc gcttcataat acacctctgt ttcacatttt ctaaaatatt
    49981 gtctcatata actgttcaat cagcaaattc actttttaat cataagaaaa ctttaaaaaa
    50041 catttttgtg taacgattac tattttttaa aatttctcaa aattcttaac tatcatacca
    50101 taccgtgaaa aagatcgaaa gaccgaatac acgcagttcg caatcgtttt atcttttttt
    50161 acgtattttt tccagaaaaa gcgaacaaca aggtattttc caaataatcg tgcagtaaaa
    50221 tgatcgcaca tacctttccc atctttttaa cagtctttgc ctaccgttca gattatcatt
    50281 cgatttcacg aaaatttctt cctatgactg gtccttttgt taaacccatc ttctattcgt
    50341 tttatttcca gctcactgca tggctcttaa aatgactcga actagtgatt tcattaccat
    50401 catcttttaa tggatagata tgctcctctt caaagtaaaa cgattttctt ttaaagtaat
    50461 agaattgaca tggtaatgag tatgtatact agtggtttca caaatggtct tttattcaac
    50521 atccaaaaac tgaaatttct ttaaaatcca ttactaacga agaaaatgtt ccttgcttga
    50581 taattagtta ggacatgtca agtgaacaaa tgcatttact tagccaaaaa atctaaaaac
    50641 ttcataaata gacaaaaaga cacgactctc taagaagtcg tgtctttatt caacaaatta
    50701 ttataattta gtcacttcag cagcttgagg tccgcgtgca ccttcaacaa tcgtgaattc
    50761 tacttcttga ccttcttcta ggctcttgaa gccgtctcct tgaattgctg agaaatgaac
    50821 aaatacgtca tctccgccgt ctactgtaat aaacccaaat cctttttcat tgttgaacca
    50881 tttaaccgtt ccgtgttcca taaatgtata gcctccaaaa aagttcttgc aagtaacctt
    50941 gcaagaacat tatacccaat ttagaaaacg ctagcaagct ttcgctcttt tattttccaa
    51001 attattttta agactgatat gacggaggaa atggttattt tgctgcttcg aaacgtttgt
    51061 ttacttcatc ccaattaata acattccaga atgctgcaat ataatctggt cggacatttt
    51121 tatatttcaa ataataagca tgttcccaaa cgtccaaacc tagaattggt gtctttcctt
    51181 ccatcaatgg agaatcttga tttgcagtag aggtaatagc taattttcca ttttccatta
    51241 caagccaagc ccatccagaa ccaaatcgtc cagcggctgc ttttttgaat tcttctttaa
    51301 aagaagaaaa atcaccaaaa gcttcattaa tcgcttcttt tatttctcct gtaggttcgc
    51361 caccagcatt tggtgccatg atttcccaga aaaatgaatg gttagcatgt ccgccaccat
    51421 tgttacgtac agctgtcttg atatctgttg gaatagcgtc catatcagat aataattctt
    51481 ctattgtttt ttcgcctaat tctgggtatt tctcaattgc tgcatttaaa ttcgtcacat
    51541 aagtattgtg atgcttatca tgatgcagat gcatcgtttc ttcgtcaata taaggttcca
    51601 atgcgtcata tgcataaggt agatctggta atgtgtaagt cataaaaaaa ttcctcctca
    51661 atgttttctt tataatgtct acattacaaa gagtaacaaa gagaaggaaa ttcttcaatt
    51721 gttatgccta gtcgtctttt tcactttgac ctacttcttc ttcaaaaacc tcagtttctt
    51781 taaaaataaa caacttgctg aaaatatagt tcgctaggat aacaataatt tgagtgatga
    51841 ttttcgccac atactcattg ctgttcataa cattgatcaa caataccatt gcccccatgt
    51901 ccaatccgta agaaaggata cggtagaaga agaacttacc gcattcaata agtgcatgtg
    51961 taaaattcgg tgattttgaa tggaataccc aaagtttgtt cgttacaaaa gcaaacaata
    52021 cagaacaaaa ccacgaaata gaattggata aggccatact taaatcaaaa tattggcgtg
    52081 cgagagcaaa actaacaaaa tttacaatcg tagccaaacc accaaaaaag agatagacta
    52141 gtatctccca atatcctttt gcttctaaat atccttttaa ctgtcgatac tgcttcatga
    52201 gccaacctac cttattaaaa attcttattt atcatactct atatttcaat aactttcatc
    52261 tttatttaaa aaaattaatt ttttttgatc ctttgccttt gtctctgctt ggatcgtcac
    52321 atgtgtaata ccatatttta attttaataa agaaattata cgaggatata tcttttcaat
    52381 ctcgcttagt agctgatctt caaaatcaat atgtccgtcg aaaataatta tcttttcgct
    52441 atactgccaa atatggacat gatgaacatt ctttacccca tcgattgctt caatttcttg
    52501 ttccattttt tcatagtcaa tagcaggtgc actttgtatc aaaatagcgg cagctttttt
    52561 gatcaccatt atcgcttcta tcaatatata tatcgaaatg atcagtgtaa taactggatc
    52621 gatccagtag atatcgaaaa aacggatcaa aattgctccg ataatcacac ttaccgaaga
    52681 cagtgtatca cttaacaaat gcaaataact agattttata ttcaaacttt catctgcttc
    52741 ttgtcgtaat aataatactg agaacaaatt cgcgagcaat ccaataactg ctactgttag
    52801 catcaaatga ctatttatct tttctggtga agtgaaccgc ttgaatgctt caacggttaa
    52861 aaacaaggat aatatgatca atatgaatga gttgatgaaa gcactcaata tttctaaacg
    52921 ctggtagcca tacgtacgtt ttttatttgc ttcttttcgg gccatttttt gtgccccgta
    52981 agaaaaaata attgccaatg tatcactaag gttatggaaa gcatctgaaa caagagacaa
    53041 actccctgac ataatccctc caataaattc tgataaagtg atcaccgcat tcaaaaccac
    53101 tactccaaat agtttttttg atgatctctg tggctgtgta ggcactattc cttcctccat
    53161 tttatttata ttggatagtc tttagacaag aataacattt ttttgatatt tcaccaatca
    53221 ttcagatgtt tccctaaagc acttgtattt cacttgatac ttcgctagaa ttgcaagtga
    53281 tgacactact agatgaggag tacttatgac aacaaaacaa ttaattatca gttcctttat
    53341 acgattcaaa gacctaaagg aggcgagaaa tgtcccattt tggaaagcca ttgtctatct
    53401 tttagttttg agtataatta tggctttgcc aatctctttt caagtttttc aagtcttaaa
    53461 taacattcaa ttggatgggc aaaagattgc tactcgcatt cctgatttta caatccaaaa
    53521 cgggcagctt caaaccgaag aaaaagaagg attcatttac caaacaaact ccattatctt
    53581 cacatttgat ccagagggaa aacgaacaga acaggacatc tctactgatt tgatggggaa
    53641 ttttgttagt gtcgggatgt taaaaaacaa acttatcata gctcttccta atacaggcac
    53701 gacttctgct ttgttgaaca ataaccaatt agaacttccc tacaccaatg aatctttaaa
    53761 aaacttaaca ggtaagcagc tccgttcttt tttaagtgaa gcatctatac cactttggat
    53821 aaaagtcctg acatttatat tttctattta tcctagtttt ctgaatctgc tgatcacttt
    53881 gctatttacc aacattgctg cttttctcta tgcacgcttt cgactaacaa aagctacttt
    53941 tcttgattgt ttaaaaacat tgatctattc aatttcattg cctactataa ttgcaacaat
    54001 cttgatggtc tttcttcctt cttttgacac tagcgccttc attgcttttg caggaatctt
    54061 tatttttgct caagctgtca aaggatgggc aaaaatatcg atcaattaag aaataaagac
    54121 aactcgttat ttctacttaa atagaccctt ttagaggttg tgacattttt ttgtcacagc
    54181 ccctttttta tttgaaaaat atttctttct caaaaaacaa gcaaggcgct tcaaagttct
    54241 tcttgtatgc gctacactta gagtaaagaa aggcgggaaa ttaactatgt cagaaagaat
    54301 catttatgca gatcgtccat ttcgtgatga atttatcgaa gaaatccata ctatagcgcc
    54361 tggttatatc tttaaaacag aattacaacc agaggatcta caaaatgtag agatcagttt
    54421 gggctggaac tcagactacc aagaaaaact attagcctct tctaccttga aatgggtcca
    54481 atctatctca gctggtgttg atcagctacc ccttgagact ttttcaaaga aacatatttt
    54541 actttcaaat ggtagtggca tccacagtga atcgattacg gagcatgtct taggaatcat
    54601 ccttgggtat tctcgtggtc tttttcaagc acaaaaggca cagagtgaca tgaaatggtt
    54661 aggttcatcg gtccattacc aatctatcca agggaaaaaa atactaatca ttggaactgg
    54721 ccatattgga aaaatgattg caaaaaaatg caaagcatta gacatgaact gctatggaat
    54781 caatacatcc ggtcatccag cagaaggaat ggaaaagact ttttctttaa atcaactaaa
    54841 ggaagttctt cctgaagtag atttcgtagt caatatctta cctctaacgg aggaaacaac
    54901 tgggctcttt gatcagcata tgtttgaaca ctttgatcca aaatctgtat tcatcaatgt
    54961 tggccgtggt gcttctgtta agacacaaga tctggtgcag gctttaaata accagcaact
    55021 ttcctttgca gcattagacg tttttgagga agagcctctt ccacaagatc atcctctttg
    55081 gaaaatggac aatgtactga ttacttccca cattgcaggt ttgacacctg attttcaaaa
    55141 gaaattaatg gctatttttt taaccaatct gaaaagctac tttgccaccc atgagcttca
    55201 aaccaatcaa gtaaaattgt ctgcaggata ttaatagctg aaaacaatat tttcaaaaga
    55261 aaaaagagaa ggtgacaaaa attgtgtcac cctctctttt tttattgttt tttaaagtga
    55321 taggttggct cctccaggtt gggatcataa tcaacaagta aatcatatcc tttaaagaac
    55381 cattcatctg cttcctcgat aaaaaaaagt gtcccatcca tttccacttt ctttacagga
    55441 tgttctggct gttcaactga cattccaatc gaaaaaccat catgaacctc tgttttgccg
    55501 tagacttttc caaaaaaacg tattccgtaa gtttctggca gatcaatttc tgagtcaaac
    55561 catttttgtg cttttggcgt taccgttaat ttcatcccaa caacctactt tctcttttct
    55621 ttctattgat tcatcttatc gtctagaata tccacatcgc ctgtctctcc aataaccagt
    55681 agattatcat tttcacgaac atattcatct gctgcaggag aagcgataac cgtttgatct
    55741 actcttcgta tggcgactac tgttaagtca aaacgttgcc tgaaatttaa ttcagctaat
    55801 gttttgttga aaaatttagg attggagact cggatttctg ctaaagaata tttatctgac
    55861 agctctaagt agtctagaat attctttgat actaagttat gtgcaatccg tatccccatg
    55921 tctcgctcag ggtgtacgac acgatctgct ccgatcttct ctagtacacg ggcatggtac
    55981 tcattttggg cttttgctaa aatattcggt acgcccattt cctttaccat cagagtaact
    56041 aagatgctgg cttggatatc ttctccaata gcgacaatta catgatcgaa atttcttata
    56101 cctaatgaac gaagtgtcat ctcatcctgt gcatttgcca caacagcatg ggtggctata
    56161 ttcatatatt cattgactcg atcttcatta ctgtcaatag ccagtacttc ttgtccagct
    56221 tcgatcagtg tacggcagat gcttccgcca aatcgtccaa ggcctacaat tgcaaaattt
    56281 tgtttcacca ttttcctcct ttagtatctt cttttctcag tataacaaag cctttccaaa
    56341 aacaaaagca agcatcatta ttgtaaaaaa gccaagaaaa ataatagtta ttttctcagc
    56401 ttttgattat cctctttatc atcgttttta tttcaccaaa ccatgtttaa acgcatagat
    56461 agctgcttga gttcggtctt ctacctctaa cttagctaat atattcgaca catgcgtttt
    56521 gactgttttc agtgtgataa acaattcatc cgcgatttcc tgattacttt tcccttcaga
    56581 aatcagcatc aaaatttcat tttctcgatt cgttaattct tcatgcaata ctggatcatt
    56641 tcgccgactc attttttcca tcatttttgt tgtcacttca ggttctagta ctcgctcgcc
    56701 gcgttgcgta gcccttatag catcagctat ttcatgtgct gtagacgtct tcaacaaata
    56761 tcctgctgct cctgcttcaa ttgcaggata aactttttca tcatcaataa aacttgtgac
    56821 gataatgatt ttggcttttg gccaatcttt taagattgct tttgttgatt cgatgccatc
    56881 catttcttcc atgactagat ccattaaaat gacatccggt cgaagtgcca tcgccttttc
    56941 atagccttga cgtccatttt cagcttctcc aatcacttca atgtcttctt ggatagataa
    57001 ataagaagag acgcctaagc gtaccatttc atggtcatct actaataaaa cttttatcat
    57061 ctttatctat gcctccttca tcaaagggac cttggtctcc acgctcgtac cttgcccttt
    57121 aaaactaatg atttttactg ttccaccaat accagctact cgttctttga tattgttcaa
    57181 tccataactt cccgttttgg tttcattcat attaaatcct gtcccgtcgt caatgatacg
    57241 taataaaaga ttgttatcta ttttatgcaa atagacttct aattcattag ccttagcatg
    57301 tcttaatgtg tttgataaca attcttgaac gattcggaac aagtgatcct caatggaact
    57361 agttaatttt acatcttcta cctcccattt tagcgaaatc tggattttat tctgcaattc
    57421 cttcaataac tgctcaatgc cttgttttaa acttttttct tctaaattga ctggacgcag
    57481 atgaagaagc agcgcacgca tctcagactg ggaagcgttg atgatctctg ccaccatttt
    57541 caactgctta cggaacattt ctggtgtctc acttttttct gcttgctccg tcaatgccga
    57601 catcatcatc atcgctgcaa ataattgctg tgaaactgaa tcatgaagct cacgagctaa
    57661 tcgatgcctt tctaatttta aaatctcttc tttggtttgt ccatctacat attgaggacg
    57721 gctagtcata atctgtaatt cactggatac ttcgatcatt ttttctttta ttttagtaat
    57781 ttccatatca atatctttga tgtacagatc atccgacgcg cttgggatcg gctgatgcaa
    57841 aagtttggat tcataattgc cagaagaaag tgcacgaagc ttttcttcga ttttcccgta
    57901 ttttgccttt tgaataagtg ataacaatag aaatacgatc aatcctacac ctaatgaaat
    57961 tgccatcaaa tggaagataa gcggaacgta taataaacgc gcacgcagca attctcccca
    58021 ccaatgactt tggttactgg cataaaaata agtgaaaagt gaaaatagaa taataaggaa
    58081 agccgcaatc cccgaataaa cagctagcat tgctttggat attcttccca tcatattcgt
    58141 atcacctcga catctccaac caaagtattg gtaatgatct tcaatctgcg aggattttca
    58201 tcataatcac tgctgcagat tttgatctgt tcgtttttta aggaaaactg ctcttcttca
    58261 aacaagacat ttcctactaa tgtcgcatgc tctaaacgaa tagccactcc caggggtact
    58321 aagatccgtg ttctgcctat cccttttctt acaatcacaa tattatcatc ttttggcaaa
    58381 agtgtattgc ccaaatcgat gatagtatca ccagaaatga tggcaatgtt gatatcatcc
    58441 cattcataga cgtcgtttcc aatacgttgg ttaccaaata attgctgtct tttacgctca
    58501 ttattatgtg tttttaattg ctctgtttgg accatcatga tctgtttttt tcgccaaaaa
    58561 gcattttttg tcaaatctat tcctgaaatc tctacacctt tcagcccgat aaataaaacg
    58621 gccaatacaa ccatcatcca taaagcagga ctgttgacta agctaaaaaa aatgaccaag
    58681 ctccctaaga tcaattgaaa attttgaaat ttagtacgag gatatttgcg catagctaga
    58741 tatatattga aaataccgaa tattactaaa agcaaaagtc cagtattgtt tacgatttgc
    58801 caaacagcaa ataacagcag cagtgcttcg actaccacaa aaaaacgcca agaactattc
    58861 atcgcttttt ctccctcctg acacattctc atttcatcat acctattttt ttgactgttg
    58921 gctataggac tttagatgta agatcgttca gtgaaacctg caatactcaa agagaaaaga
    58981 tctagagtgc gtcatcagta agatgaactc tctagatctt tcagtcagac tacgcttgtt
    59041 ctactttgac gattttaact aacatatcgc cacctggtgt agcaatggtt acttcatcat
    59101 ttttacgttt gccaatcaat gcttgcgcga taggagaatc attggaaatc tttccagaaa
    59161 acgggtctgc ttcagcactt ccgacaatcg tgtactcttc ttcatctcca tcaggcaact
    59221 cgatgaacgt aactgtttta ccgatcgata cctcatctgc atctacgccg tcattatcga
    59281 tgatttgtgc aaaacggatc atattttcaa ttgttgtgat tcttccttca acaaatgctt
    59341 gttcatcttt agctgattcg tattcggagt tttcggaaag atctccgaaa ccgcgagcga
    59401 ttttgattcg ttcaatgatt tctccgcgtt taactgtttt tagttcttct aattcttttt
    59461 ctaatttttc tttaccttct agagtcattg gataaacttt ctctaccatg tgtaacgtct
    59521 ccttttttcg ataaaatacc gcaaaaggta atcttttgag gaatatagtc caaaaaagat
    59581 taccacgctt ttcttaaaat gtaaatgttt ttctaaaaat gatcaatgac tagcccctgt
    59641 ttttctaaga attatttaca tatttttgca ctagttccat atgttcttca tacgttttag
    59701 cgaaataaac atttccagtg ctcgtatctg ctacgaaata ataatagtca ttttctgctg
    59761 gttctaatac tgccttgatt gcctcttcgc ttggattgtc aaaaggacca ggcccatagc
    59821 cggtattggt atacaaattg tatggtgaat ctacagctgt atcttcatac gtcaccaatt
    59881 ctttatgttc tcctaatgca tataaaacag agatatctga ctgaagtggc atatttgcct
    59941 tgattcggtt aaagaagact tgtgcaatat ttttacgatc attttctttt acgccttctt
    60001 tttcaactag agaagccaaa gttaaaactt cttgtacagt caaatttttc tgtttgatag
    60061 ctgaataata gttttgcatg accgtatttg ttttgttcac catctcaatc acaagatcct
    60121 taagcgaagt actcgtgtag taatcataag tagctgggaa taagtagcct tccaaacggt
    60181 attttgtgtt ctcagcttta gaggcacttt ccaacaattc tgggaatttt tgatacaact
    60241 cgttaaagaa agtctcatca tttactaaat ctaaaaattc tttcttgtct ttaccagtta
    60301 ccttcgctac ccgctcagca atctgatcga tgtcgtagcc ttctggaatg gcaattttcc
    60361 catctgcaac ttttgttggc tcagaagtgc cgccttcttg cagctgtttg ccaatctcgt
    60421 caagagtcat attaggcgct aactgataat agccagcttg aaagccagtt aaatttttaa
    60481 attttgtata ataattgaaa acaatcccgc ttttaataat attgtccttc tccaaaatct
    60541 caccaatctg cttgttagaa gagccactcg ggatctccac ttggactaat tggtcatctg
    60601 atttatctaa aggcttcaac ccgctatcta cataacgata cacggtaaat ccaagaaatc
    60661 cgccaataat taataatgta agagcaatca ctaaaatgat cttcctcaca atcttatctt
    60721 cttttttacg cagatttctc cgcttgtcga ttatatctgc tgtagctgcc ttactggctt
    60781 cttttttatc tttacttggg tgttgtttgt ttcctgacaa aacaactcct ccttactatc
    60841 atcttcgtaa ctctgtttat tatacatgaa atttctactg ccgtgaatta ttttaccaaa
    60901 tattatgtca aaattgtaag tggcaaatac tctttttgag gattacaatg aaaaatagtt
    60961 aaaaatggat agaaaggaat taacatgtca aaaggaaaac atcgaaaaaa acaaaaaatt
    61021 tctatttctt ttcttgtaat tttattattg tctgtcagct tcttttcaac aggcatcatt
    61081 ataggaggtc aattaacaaa aatacctcat tcccaaactt ataaagcgga gccaacacaa
    61141 cttacttcca atttaacgcc cgatgagcag aggcttgcta gcaaaattga tcaattacta
    61201 caaaaatcag attatatcgg ttcgatttat gttcgcaaaa ataatcgcgt actattagaa
    61261 aaaggatatg gatatgcaaa tctgcgcgta aaagaatcga attcaccttc tatttactat
    61321 caaatcggtt cgattcaaaa agcaatgact gctttactga tactaaaaca aatccaagct
    61381 ggtaagttaa cttttgacac aaagttagca gaattttatc ctcagattcc tggaagtcag
    61441 caaatcacaa tcaaaaatat gctttatatg cgctctggtc ttcatcgaac tgcttctccc
    61501 gacaaaccta tgtcggatag tgaaatcatt cagttcgccc ttcatcattt gaaacttact
    61561 gattatgata cgtatcatta tgaaccattg aattatacat tgcttacagg gatattgatc
    61621 aaactaacga atcagcctta tgaaactctt ctaaaaaaag aaatcattcg tccactccat
    61681 ctagaacata cagattttta tgacaatgta aaaaattcgc ctcaacatgc tgtatcttac
    61741 cagatgtctg caactgacga ttattcaaaa gcattgatcg agcctgaaac cgacatccga
    61801 aatgaattag gtacaggaaa cataagtatg tctgtttatg acttgaacaa attctttacc
    61861 agtgttcttt ctggcggtat cataccaaaa gaactacttt tctcattgtg gcaaaataat
    61921 ggtgataaac atccttacag cggcggtgtt tacagtggaa acgattatat ccttgctcag
    61981 gggaatatca atcgattcca tgcagtagtg gcgatgaaaa aagacactaa agatgcaatc
    62041 gtcatggaaa gtaatatcca atcggataag aacatcaaat tgccggccac tgatttaaga
    62101 aatcagattt atgaactgat ggaaggtgtt cacttatcaa attaaatttt gacataatat
    62161 tagttgtcat ctctgtatag tcaatttagt tagtactcta tctgaaaagt agaaataaat
    62221 taacaaatat tatctgtcct tattatttgt taatttattt cttatttta
//
LOCUS       DANKEF010000012        59949 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000012 DANKEF010000000
VERSION     DANKEF010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59949)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59949)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59949
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.012"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(137..514)
                     /locus_tag="PWZ48_000837"
     CDS             complement(137..514)
                     /locus_tag="PWZ48_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260511.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            770..1498
                     /locus_tag="PWZ48_000838"
     CDS             770..1498
                     /locus_tag="PWZ48_000838"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDL2260512.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            1498..1752
                     /gene="purS"
                     /locus_tag="PWZ48_000839"
     CDS             1498..1752
                     /gene="purS"
                     /locus_tag="PWZ48_000839"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HDL2260513.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            1754..2425
                     /gene="purQ"
                     /locus_tag="PWZ48_000840"
     CDS             1754..2425
                     /gene="purQ"
                     /locus_tag="PWZ48_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HDL2260514.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            2426..4648
                     /gene="purL"
                     /locus_tag="PWZ48_000841"
     CDS             2426..4648
                     /gene="purL"
                     /locus_tag="PWZ48_000841"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HDL2260515.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            4633..6072
                     /locus_tag="PWZ48_000842"
     CDS             4633..6072
                     /locus_tag="PWZ48_000842"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDL2260516.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            6095..7147
                     /locus_tag="PWZ48_000843"
     CDS             6095..7147
                     /locus_tag="PWZ48_000843"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDL2260517.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            7144..7722
                     /gene="purN"
                     /locus_tag="PWZ48_000844"
     CDS             7144..7722
                     /gene="purN"
                     /locus_tag="PWZ48_000844"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDL2260518.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            7719..9254
                     /gene="purH"
                     /locus_tag="PWZ48_000845"
     CDS             7719..9254
                     /gene="purH"
                     /locus_tag="PWZ48_000845"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDL2260519.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            9270..10544
                     /gene="purD"
                     /locus_tag="PWZ48_000846"
     CDS             9270..10544
                     /gene="purD"
                     /locus_tag="PWZ48_000846"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDL2260520.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            10666..11580
                     /locus_tag="PWZ48_000847"
     CDS             10666..11580
                     /locus_tag="PWZ48_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2260521.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(11713..13278)
                     /locus_tag="PWZ48_000848"
     CDS             complement(11713..13278)
                     /locus_tag="PWZ48_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2260522.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSYFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(13271..14722)
                     /locus_tag="PWZ48_000849"
     CDS             complement(13271..14722)
                     /locus_tag="PWZ48_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2260523.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(14736..15752)
                     /locus_tag="PWZ48_000850"
     CDS             complement(14736..15752)
                     /locus_tag="PWZ48_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2260524.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            15972..17060
                     /locus_tag="PWZ48_000851"
     CDS             15972..17060
                     /locus_tag="PWZ48_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HDL2260525.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            17114..18640
                     /locus_tag="PWZ48_000852"
     CDS             17114..18640
                     /locus_tag="PWZ48_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDL2260526.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            18642..19847
                     /locus_tag="PWZ48_000853"
     CDS             18642..19847
                     /locus_tag="PWZ48_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2260527.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            20104..21081
                     /gene="guaC"
                     /locus_tag="PWZ48_000854"
     CDS             20104..21081
                     /gene="guaC"
                     /locus_tag="PWZ48_000854"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDL2260528.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            21345..22769
                     /locus_tag="PWZ48_000855"
     CDS             21345..22769
                     /locus_tag="PWZ48_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDL2260529.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            23230..24339
                     /gene="pdhA"
                     /locus_tag="PWZ48_000856"
     CDS             23230..24339
                     /gene="pdhA"
                     /locus_tag="PWZ48_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HDL2260530.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            24342..25319
                     /locus_tag="PWZ48_000857"
     CDS             24342..25319
                     /locus_tag="PWZ48_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HDL2260531.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
     gene            25344..26987
                     /locus_tag="PWZ48_000858"
     CDS             25344..26987
                     /locus_tag="PWZ48_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDL2260532.1"
                     /translation="MAYQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAAESQTPAKPAA
                     EPTVDTESAGSSSEGVFQFKLPDIGEGIAEGEIVKWFVKPGDTINEDDTLLEVQNDKS
                     VEEIPSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSAEAPKEKVETS
                     GSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKEDIENFLAGGPS
                     SAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPTRKAIAKAMVN
                     SKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALTATVKKYPVLN
                     ASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEINEKAKLAHDG
                     KLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIVNAEGEIVVGR
                     VMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            26995..28401
                     /gene="lpdA"
                     /locus_tag="PWZ48_000859"
     CDS             26995..28401
                     /gene="lpdA"
                     /locus_tag="PWZ48_000859"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDL2260533.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            28563..28838
                     /locus_tag="PWZ48_000860"
     CDS             28563..28838
                     /locus_tag="PWZ48_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HDL2260534.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            28844..29611
                     /locus_tag="PWZ48_000861"
     CDS             28844..29611
                     /locus_tag="PWZ48_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HDL2260535.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            29779..31608
                     /gene="typA"
                     /locus_tag="PWZ48_000862"
     CDS             29779..31608
                     /gene="typA"
                     /locus_tag="PWZ48_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HDL2260536.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            31869..33641
                     /gene="recQ"
                     /locus_tag="PWZ48_000863"
     CDS             31869..33641
                     /gene="recQ"
                     /locus_tag="PWZ48_000863"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HDL2260537.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            33755..34057
                     /locus_tag="PWZ48_000864"
     CDS             33755..34057
                     /locus_tag="PWZ48_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HDL2260538.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            34060..35223
                     /locus_tag="PWZ48_000865"
     CDS             34060..35223
                     /locus_tag="PWZ48_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2260539.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            35249..38677
                     /locus_tag="PWZ48_000866"
     CDS             35249..38677
                     /locus_tag="PWZ48_000866"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HDL2260540.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            38698..39822
                     /locus_tag="PWZ48_000867"
     CDS             38698..39822
                     /locus_tag="PWZ48_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260541.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            39812..40249
                     /locus_tag="PWZ48_000868"
     CDS             39812..40249
                     /locus_tag="PWZ48_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2260542.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            40233..40571
                     /locus_tag="PWZ48_000869"
     CDS             40233..40571
                     /locus_tag="PWZ48_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HDL2260543.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            40690..41250
                     /gene="rsmD"
                     /locus_tag="PWZ48_000870"
     CDS             40690..41250
                     /gene="rsmD"
                     /locus_tag="PWZ48_000870"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HDL2260544.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            41247..41738
                     /gene="coaD"
                     /locus_tag="PWZ48_000871"
     CDS             41247..41738
                     /gene="coaD"
                     /locus_tag="PWZ48_000871"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDL2260545.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            41728..42771
                     /locus_tag="PWZ48_000872"
     CDS             41728..42771
                     /locus_tag="PWZ48_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HDL2260546.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            42930..43658
                     /locus_tag="PWZ48_000873"
     CDS             42930..43658
                     /locus_tag="PWZ48_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDL2260547.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            43774..44019
                     /locus_tag="PWZ48_000874"
     CDS             43774..44019
                     /locus_tag="PWZ48_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2260548.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            44016..44234
                     /locus_tag="PWZ48_000875"
     CDS             44016..44234
                     /locus_tag="PWZ48_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDL2260549.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            44370..44909
                     /locus_tag="PWZ48_000876"
     CDS             44370..44909
                     /locus_tag="PWZ48_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HDL2260550.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            44902..47175
                     /locus_tag="PWZ48_000877"
     CDS             44902..47175
                     /locus_tag="PWZ48_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HDL2260551.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            47213..48259
                     /gene="holA"
                     /locus_tag="PWZ48_000878"
     CDS             47213..48259
                     /gene="holA"
                     /locus_tag="PWZ48_000878"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDL2260552.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFASYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(48348..48602)
                     /locus_tag="PWZ48_000879"
     CDS             complement(48348..48602)
                     /locus_tag="PWZ48_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDL2260553.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(49002..49271)
                     /locus_tag="PWZ48_000880"
     CDS             complement(49002..49271)
                     /locus_tag="PWZ48_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260554.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(49741..50196)
                     /locus_tag="PWZ48_000881"
     CDS             complement(49741..50196)
                     /locus_tag="PWZ48_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDL2260555.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(50334..52262)
                     /locus_tag="PWZ48_000882"
     CDS             complement(50334..52262)
                     /locus_tag="PWZ48_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2260556.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
     gene            complement(52459..53292)
                     /locus_tag="PWZ48_000883"
     CDS             complement(52459..53292)
                     /locus_tag="PWZ48_000883"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDL2260557.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(53484..54188)
                     /locus_tag="PWZ48_000884"
     CDS             complement(53484..54188)
                     /locus_tag="PWZ48_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2260558.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(54211..54837)
                     /locus_tag="PWZ48_000885"
     CDS             complement(54211..54837)
                     /locus_tag="PWZ48_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2260559.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            55163..57538
                     /locus_tag="PWZ48_000886"
     CDS             55163..57538
                     /locus_tag="PWZ48_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDL2260560.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(57562..58053)
                     /locus_tag="PWZ48_000887"
     CDS             complement(57562..58053)
                     /locus_tag="PWZ48_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HDL2260561.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            58240..59160
                     /locus_tag="PWZ48_000888"
     CDS             58240..59160
                     /locus_tag="PWZ48_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2260562.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            59314..59658
                     /locus_tag="PWZ48_000889"
     CDS             59314..59658
                     /locus_tag="PWZ48_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HDL2260563.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(59848..>59949)
                     /locus_tag="PWZ48_000890"
     CDS             complement(59848..>59949)
                     /locus_tag="PWZ48_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260564.1"
                     /translation="NKKNLGSLKAQQKNGAAIYREDEKEYYTKIKLM"
BASE COUNT        19672 a        10363 c        12872 g        17042 t
ORIGIN      
        1 ataaccaaaa aagaagcaga tccgttagcg gttctgcttc ttttagttta ttatattttc
       61 ttatcaataa taggaacctg attttaaaag cggctacctc tttaccgtgc ctctttacat
      121 acaaaaaata gaggaattaa gcgtaaatgg cttggctatt ataactaaaa tgctggttat
      181 ggagatgttg cagcaagttt tcgagaaaag cgtattccat ctcttgtcca ttgaataaga
      241 tctggatacg ataatgattt ttgaaacgct tctgttcatg aatctgactg attgaaagtt
      301 cgcctttgtg ttctgtctga taaagaaaag tccttttacg gcattcagca cagcgcttgg
      361 ttgctcggcg gatttttcgg aatgtataaa aactagtatc tttattgcat aaataaacgt
      421 ctttgatcga cagcaaatca ttatatagac aggaacgttc ttcagctgtt aattctgata
      481 aaaaaagctg accttgtttt attgcatctt ccatcggcaa gtgcctcctc gaacccatta
      541 tccaaatttt tagttaattc taatatacca tcattaagta taaatgtaaa cgcttgttga
      601 tggtataaga ttttcgtttc tgataaaaca cgaacattaa aagaatctaa tgccttaaat
      661 gtacatattt ttgttgattt ataaaaagat gattgctaaa atttaaagtg aaaaaacgaa
      721 caaaaaacga tgtatttgct tttaatgtaa ataaaaagga gagacgtaca tggagaagaa
      781 agaacttttg tatgaaggaa aagccaaaaa aatatatctt acttctgatg agaacttgat
      841 ttggatagaa tatttagatc aagcaactgc gttgaatggc gcgaggaaag accagataag
      901 tggaaaagga gaattgaata atcaaatcac aagtgcaatt ttccgttatc tgactcaaag
      961 aggaataaaa aatcatttta ttgaacaatt gtctaaaacc gaacaattag caaaaaaagt
     1021 acaaattatc cctctggaag tggttatccg aaatattgca gcaggaagtt tctcaaaacg
     1081 tctaggtgtt cccgaaggta aagaattcat caaaccaatc attgaattct actataagga
     1141 tgaccagttg gatgatccat ttatcaatga agagcatatc tatttcttac aacttgcaaa
     1201 gccggaagaa atcagtgagc tgaaagccca agcgttggct attaatgaaa ttttgaccga
     1261 attatttgct aaggtcggtt tggttttagt agatttcaag ctagaatttg gccggtcgaa
     1321 tggacaaatt ttgcttgcgg atgaagtaac accggatact tgccgattat gggatgaacg
     1381 tacgaatgaa catatggaca aagacgttta ccgcagagat ctaggtgatt tgatcccagt
     1441 ctatcaagaa gtatataacc gtctatcgga actaactgaa gaaaaggagc gagtatgatg
     1501 tatttcgtaa aagtatatgt aacctataaa gattcagtgt tagatcctca aggggaggca
     1561 gtaaaaaaag cagtccatcg tatgggatat gaaacaatcg aagatatccg aataggaaaa
     1621 tactttgaga tacaacttgc tgatgcagaa gaaccaatcg atacagtgat tgaaacgatt
     1681 tgtgacaagc tgcttgcaaa tgtaaatatg gaaacatacc gatatgaaat caaaaaaatg
     1741 gaggaagtat aacatgaaat ttgcggtcat cgtttttcca ggttcaaatt gtgacgcaga
     1801 tatgttatgg gcagttcgcg agatcatggg cgctgatgcc gaatttgtac ggcatgatga
     1861 gtcctcttta gaaggatttg atggggtttt actgccggga ggattctcct atggtgacta
     1921 tttgagatgc ggtgcgattg ctcggttttc tacaatcatg gaagaagtga ttcgttttgc
     1981 tgaagaaggg aaaccagtat tcggtacatg caatggattc cagatattga cagaagcagg
     2041 acttttgcca ggtgtactga gacgaaatga gtctttgcat tttatttgca aaactgtacc
     2101 attgaaagtc aaccccaaga cggcctttac ttcagagtat gaagccgacg aaataatcta
     2161 tcttccagtt gctcatgggg aaggaaatta tttctgtgac gaagaaacac ttcaagaact
     2221 aaaaaaaaat aatcagattg cctttactta taatgacaac atcaacggca gcgtggaaaa
     2281 cattgcaggg attacgaaca aagcagggaa tgtgttagga atgatgcctc atccagaacg
     2341 tgcagtagaa gacttgctag gatcagaaga tgggaaacgg ttctttgctt ccattttaaa
     2401 aaacttcgga aaggtgaccg tatagatgaa aacattgaaa gaaccgacag ctgaagagat
     2461 caagaaaaaa agaatctatg ccgaatgggg attgactgat gaagaatatc gaatgatcga
     2521 agaagatatc ttaggaagaa tgcctaatta tacggaaact ggactgtttt ctgttatgtg
     2581 gagcgaacat tgttcctata aaaattctaa accggttcta cgcaaatttc ctactagtgg
     2641 acctcatgtg ttgcaaggcc ctggagaagg cgcaggcatc gtcgacatcg gtgatggact
     2701 agcagttgtc ttcaaagcag aaagtcacaa tcatccttca gccgtggagc cttatgaagg
     2761 agctgcgaca ggagttggcg gaatcatcag agatattttc agcatggggg ctcgtccaat
     2821 tgcgttgcta gattcattga ggttcggcga attagataat ccaagaacga aatatttact
     2881 agaagaagta gttgcaggta tcggcggata cggcaattgt atcggcatac cgactgtagg
     2941 cggcgaaatt gcatttgatc cttgttatga aggaaaccca ttagtcaatg caatgtgcgt
     3001 aggtttgatt gaacacaaac atatccagaa agggcaagcc gctggcgtcg gtaattcgat
     3061 catgtatgta ggtgcaaaaa caggtcgcga cggtatccac ggagcaactt ttgcctcaga
     3121 agaatttaat caagaagaag aacagcaacg ttctgctgta caagtaggcg atccttttat
     3181 ggaaaagctg ctgcttgaag cgtgtttgga attaatcttg catcattcgg attctctaat
     3241 aggtatacag gatatgggag cagcaggact tgtttcatct agtgcggaaa tggcttcaaa
     3301 agctggctca ggacttatat tgacattaga tgaagtccca caaagagaaa ctgggatgac
     3361 accttatgaa atgatgttat cggaatcaca agaacggatg ttgatttgtg ttaaatcagg
     3421 agaggaagaa aaaatccaag aactatttca gaagtatgat ctagatgcag tcactatcgg
     3481 taaagtaaca gacgacgggc aatatcggct atatcatcaa ggagaagaag tagcaaatct
     3541 gccagtcgat gcattagctg aagatgcacc agtttatcat aaagaaatga aagaaccagc
     3601 tcgaatcgta gaatttcagc agttagctcc ttatcagcca gttatcgaag aacctggaca
     3661 agtattgctt gatctgctgc agcaaccgac gattgcttca aaacgttcaa tctacgaaac
     3721 ttatgattca caagtacaaa ccaatacagt tgtccgtcca ggaagcgatg ctgctgtgat
     3781 gagggttaga gggacgaata aagcgttagc tatgaccaca gactgcaacg cgcgctatct
     3841 gtatttggat ccaaaaatcg gaggacaaat agcagtggca gaagctgcta gaaatattat
     3901 tgctagcggt ggtaagccct tggcgatcac cgactgcctg aactatggtt ctcctgataa
     3961 accagaagtt ttttgggaat tatcgacttc agctgacgga attgctgctg cgtgtgaaac
     4021 gttaggaact cctgttattt caggaaatgt ctctttatat aatgaaacgg atggtcaagc
     4081 aatctatcca acaccaatga ttggtatggt cggattgatt gaggatcata aacatatcac
     4141 tactcaggaa ttcaaaaaat caggggatct aatctatata ttaggtaaga cgtttgcaga
     4201 ttttgatggc agtgaattac aaaagatgca gcttggaaga atcgaaggtg tgatccgaaa
     4261 ctttgatcta tccattgaaa agagaaacca agagttagta ttgacagcca tccaaaacgg
     4321 attgattgaa agtgctcatg actgttcaga aggtggcttg gcaattgcac tggcagaatc
     4381 agctttcaaa catcagctag ggatatctgt acagtttgaa ttgtcctctg ctcaattatt
     4441 tgcagaaact caatcaagat ttgtgttgac agtcgcaccg gaaaataaga cacgattcga
     4501 agaaatgatg ggagatgctg ctgttttagc aggtaaggtg acggatgaag cgataatcga
     4561 aatttcagca actgacggtc agatcaaaat agaaactgca gtagcaagaa agtgctggga
     4621 ggatgcaatc gtatgtctta tgaagtgaag agtttaaatg aagaatgcgg gatcttcggt
     4681 gtttggggtc atccagacgc cgctagtgtg gcatacttcg ggctccatag tcttcagcat
     4741 cgaggacaag aaggtgcagg tatcgtgtcc aatgatcgag gaaagcttaa ggggcatcga
     4801 gatttggggc tgctttctga ggtattcaaa gatcaaagaa aattgcagca gttaacagga
     4861 gaagctgcaa tcggacatgt acgttatgca acagcaggca atggaagtgt ggacaatatt
     4921 cagccttttc tattcaaatt tcatgatcag tcgatcggct tagcgcataa tggtaacttg
     4981 atcaatgctc gttcccttag aaaagagtta gaggaaaatg gggcgatctt ccattctaac
     5041 tccgatacgg agatattgat gcatttgatc cgacgaagtg aagaaacaga ttttttaaat
     5101 aaattaaaaa gtgctttgaa tgaagtcaaa ggtggctttg cctatttatt aatgacagaa
     5161 acggcaatga ttgccgcttt ggacccaaat ggttttcgtc cattagcaat cggacaaatg
     5221 aaaaacggag catacgtgat tgcctcggaa acttgtgcat tagaagttat tggggcacgt
     5281 tttataagag atgtccagcc tggtgagata gtgatcatca atgacgaagg aatccagatt
     5341 gattgcttta cgaaggacac gcagctttct atttgttcga tggaatatat ctattttgcc
     5401 agaccagatt caaacatcgc agggatcaat gtgcatactg cacggaaaaa tatgggacgg
     5461 aatttagcga aagaatcgcc agtaaaagca gatatggtcg ttggcgtacc taattcttct
     5521 ttgtcagccg caagcggtta tgcggaagct agtggaatcc cttatgaaat cggattagta
     5581 aaaaatcaat acattgcccg aacatttatc cagccaacgc ctgagctaag agagcaaggt
     5641 gttcgaatga aattatctgc tgtacgagga gtagtggaag gtaaaaaggt gattatggtc
     5701 gatgattcaa ttgttcgggg tacgacaagc agacggatcg tccatctttt gaaagaagca
     5761 ggggcaaaag aagtccatgt gaggattgct tctcctcctt tgaaatatcc atgtttttac
     5821 gggattgata ttcaaacgcg taaagaactc attgcggcaa atcacacgat tgatgaaatc
     5881 aaagaatgta tcggtgcaga ttctttaggt tttttaagtg aagagggtct gatcgaatcg
     5941 atcggtctgg aaaaagatgc tccgtattca ggattgtgta tggcttattt caacggagat
     6001 tatccgacgc cgttatatga ctatgaagaa aaatatcagg aatcactaaa agaaaaagta
     6061 tcgttctttt aaaagcagac caagaaggga gtatatgaat tcaatggcaa atgcatacgc
     6121 aaaagctggc gtagatgtcg aagctggata tgaagtagta gaacggatca agaagcatgt
     6181 caaaaaaacg gaacgtttag gtatgatggg cgctatagga ggattcggcg gttgttttga
     6241 tttatcggtc tttcaattaa aagagccagt ccttgtctcg gggaccgatg gcgtggggac
     6301 gaaattagca gtggcaatcg aagcagacaa acacgataca atcgggatcg actgcgtagc
     6361 gatgtgtgtc aatgatatct tagcacaagg cgcagagccg ttatatttct tagactactt
     6421 agcaacgggg aaaaatgatc cgttacgttt ggaaaatgta gtaaaaggtg tagcagatgg
     6481 atgtgttcaa ggaaatatgg cgctgattgg cggagaaaca gctgaaatgc ctggtatgta
     6541 ctcaggtaat gattatgatc tagccggatt tgctgtaggg atagctgaaa aatctcaatt
     6601 attgacagga gaaacgatca acccaggaga tgtgttgctt ggaatagcat ctagcgggat
     6661 tcattcaaat gggtactctc ttgttcgcaa aattttcttt gagcagcagc agttttcttt
     6721 atcatcaaaa ctgccagaat tgaaaaaaac acttggcgaa gaattgcttg agccaacgaa
     6781 aatctatgtt tcttccttgc ttccgctgat caaagaaaag aaaataaaag gagctgcaca
     6841 tatcactggt ggagggtttg tagaaaatat cccgcgaatg ttaccagaat ctatggctgc
     6901 acaaatcaaa cttggctcat ggccgattct tccaatattc ggctgtttac aaaaatatgg
     6961 tgaaattcct gagatggaga tgtatgaaat cttcaatatg ggcatcggta tggtcctggc
     7021 agtcgaagaa tcgaaagtaa aagaaatagt acacgaaatt gagcaagctg gcgaaaaagt
     7081 ttatgaaatc gggaaagtga tcgccaaaga aaaacatcgt gtggtgttta taggagaaga
     7141 acgatgagaa tcgctgtttt tgcttctgga aacggaagca actttcaagc gctagccgat
     7201 tatctatcga aaaaaggatt ggaatcttcg attgactggt tgttttgtga tcagcctgaa
     7261 gcatatgttt tgaaacgtgc aactgcgttg agtgtaccgg cagattgttt ttcacctaaa
     7321 gaatttgatt caaaaaaaga gtatgaagaa gcaattttgc ataaattgaa agaaaaaaag
     7381 atcgatttga tcgtattagc tggatatatg cgaatcattg gaccggtatt gctgaaaaat
     7441 tatgataaaa gaatcatcaa tatccatccg tctcttttac ctgccttccc tggtttgcat
     7501 ggcatacgtg atgcctttga agctggagta aaagaaacgg gtgtcacaat tcattatatc
     7561 gaccaaggtg tagatactgg accaatcatc cggcaggaaa aagtgagaat tgaacaggag
     7621 gacacgttcg attctttaga agaaaaaatc catagagtag agcatcgaat ctatccagaa
     7681 gtgattagtg aaattattga aaatggaggc tatctgaaat gacaagagca ttgatcagtg
     7741 tatcagataa aactgggatt gtagcattcg cccaaggttt gattgaacaa ggagtagaaa
     7801 tcatttcaac aggaggaaca aagcatgcgt tggataaagc tgggatcaaa acgctttcga
     7861 tcgaagaagt gacagggttt cctgaaatga tggatggccg tgtcaaaaca cttcatccta
     7921 aaatccatgg tggtctttta ggaagacgcg atctccctag ccatatggag gcaatggaaa
     7981 cggaaaatat ccaaccaatc gactttgttt gtgtcaattt atatcctttc aaagaaacga
     8041 ttatgaaacc agatgtcgaa atagcagaag ctattgaaaa cattgatatc ggtggtccta
     8101 gtatgctccg tagtgcagca aaaaaccatg aatttgtcac ctctgttgtt gatccatctg
     8161 actatgaaga agtactttca gaattacgtc agtatgggca tacttcactt tctttcagac
     8221 aaaaactagc ggcaaaagtc tttcgtcata ctgcggcata tgatgcactg atcgctcaat
     8281 acttaacgga ttgggtggaa gaaaaagaac cggaaaaatt gacacttacg tatgaacgaa
     8341 aacaaacgct tcgttacggt gaaaatagcc atcaagccgc tgccttttat cagtccgctc
     8401 ttctggaaaa atattcgatc gcagcagcaa aacaactcca tgggaaagaa ctttcttata
     8461 ataatatccg agatgcagat gctgcgctac ggatcatgaa ggaatatgac gagccaacgg
     8521 tagtcgcctt gaaacacatg aatccttgtg gtattgggac agcatctacg attttagaag
     8581 cgtgggaagc agcgtatgaa gcagatcctg tttcaatctt tggtgggatc atcgttttga
     8641 atcgagaagt agatctgcca acagcagaac agatgcataa gctgtttcta gaaatcatca
     8701 ttgccccttc ttatacacaa gaagctttgg aattactgca aaaaaagaaa aacatccgat
     8761 tactgacagt agactttgcg caggaagaaa atcaaaatcg agaagaaacg gtctcagtct
     8821 taggtggact tcttgtacaa gatcaagatc tggcaataga aaatgaagaa gagtggaaag
     8881 tcgtgacaaa acgtaagccg actccagcag aagcaaaagc attagcgttt gcttggagag
     8941 ctgtcaaaca tgtgaaatct aatgcgatcg tactggcaaa cgaacgtcag acagtaggaa
     9001 tcggagccgg gcagatgaat cgagtcggtt cagtaaaaat tgcgattgaa caagcgcaag
     9061 agaaaatgga aggtgcagtt ttagctagcg atgcttattt tccaatggat gattcggttg
     9121 aatatgcagc caaacatggt ataaaagcaa tcattcagcc aggaggaagc atcaaagacc
     9181 aagattctat tgatatggcg aataaatacg atctagcaat ggtctttact gatgttcggc
     9241 attttagaca ttagagagga gtcggataaa tgaacatttt ggtgatcggt gcaggtggtc
     9301 gtgaacatgc aataagtaaa aaacttttag ccagtccgaa ggtggatacg gtgtattgtg
     9361 cgccaggaaa tccggggatg acaaaagatg gtatcaattg tgtcgctatc agtgagcgag
     9421 atcattcagc gttaatccat tttgttaaga aagaggctat tgattggagt atcgttggtc
     9481 ctgaagttcc tttgttaaat gggatcgtcg atgattttat ggcggaagga ttgaaaatct
     9541 tcggtccgac aaaagcagca gctctgatcg aaggttcgaa aagttttgct aaacaattaa
     9601 tggatactta tcaaatcccg acagctaaat ccagaagttt tgaatgttac gaaaaagcag
     9661 cagcgtatat caaagaaaat ggtgcgccaa tcgtagtgaa agcggacggc ttagcagctg
     9721 ggaaaggtgt agtcgtggct accacagtga aggaagcgct ggatgcggca gaacaaatgt
     9781 tgaaccagca tcgatttggg gatagtagca aaaaaatcgt cgtagaagag tttttggcag
     9841 gagaagaatt ctcccttttg gcgtttgtcc gtaataccaa ggtgtatccg atggtcattt
     9901 cgcaagatca taagcgggca tttgataacg ataaagggcc aaatacaggc ggaatgggtg
     9961 cttattcacc agtaccgcaa atttcaaaaa agatgattga aaaagctgtg gaagaagttt
    10021 taaaaccagc ggctttggga atgaaagaac taggacgctc tttcacgggt gttttatatg
    10081 cgggtttgat tgctacgaag gaaggaccaa aagtgatcga attcaatgcg cgtttcggtg
    10141 atcctgaaac gcaagtcgtt ctttctcggt tgaagagtga ctttgcgcaa gtaatcgatg
    10201 atttattaga gaatcgtaca gtagagttaa agtggcagca agaaggatac aatgtaggcg
    10261 ttgtggttgc agctgccggt taccccgaag aatatgagac aggaatggtt ctaccagatt
    10321 ttctggaaga agagctttct gtttactatg ctggagtttc ctcaaataac ggcaaactag
    10381 ttggagcagg aggacgtctt tatttagtag aagcatacgg tgagacgatc aaagaagcac
    10441 agaaaaaaat ttacaccgcg ttaatggata aaacgacaga tggaacattt tatcgtacag
    10501 atattggaga aaaagctgcc aatagcgaac tgatcgtgaa gtaaatcagc tgcttccatt
    10561 tataaaaaat acaacaaaca taatcggaag cgccgatttt agtgaagcct tttcataaag
    10621 agtgtggtac acttttgttg tgtatataaa taaggaggag ttcagatgaa aaaaattcaa
    10681 ttaggtactt ctgatcagaa agcagctgca gtgatcttag gctgtatgcg tatcaacaat
    10741 gcggaagatc cagtaaaagt gattgagaca gcttatgaaa acggcattga tttttttgac
    10801 catgcagata tttatggaaa cggtgaatgt gaagaaatct ttgcagatgc tctagcaaaa
    10861 acatcaatca aacgagaaga tctttttatt caaacaaaat gtggaatcgt tccaggtgtg
    10921 atgtttgatt tttctaaaaa acatatcatt gaatctgtag aaaaaagttt gacccgtctg
    10981 aaaatggatt atgtggatgc tttacttctt catcgtccag atacattggt agaaccagag
    11041 gaagtagctg aagcgtttga tgaattggaa cggtcaggaa aagttcgtta ttttggcgtc
    11101 agcaatcaaa aaccaatgca aatcgaattg ctgaaaaaat atgttcgtca accattagta
    11161 gctaatcagc tgcaatttgg cttgaaacat actggaatga tcgatcaagg gattcatgtc
    11221 aatatgacag atgaagcaag tatcgatcat gacggtagtg tacttgatta ttcccgattg
    11281 catgatatga cgatccaagc atggtcacca taccagtatg gattttttga aggtgtattt
    11341 atcgggaacg aaaaattccc tgaattaaac caaacattag aagaactagc agctaaatat
    11401 caaacgacac caactggctt ggcttctgct tggattttac gccatccagc caatatgcaa
    11461 gtgatcgccg gtacgatgaa cgtagggcga atcaaggaaa tcgctcaagc ttcagaaatt
    11521 aatatgtctc gggaagattg gtacgaattg taccgtgctg ctggaaacat tttaccttaa
    11581 agattgtcat aagcaggtca aaagggctat aaattaaaaa gacagaataa cttgaaagct
    11641 gttttcagct tcgagatatt ctgtcttttt ttattgcaaa tggaaagagt atcattgtac
    11701 ggatttcgtt tattagttat ttttgttttt aaggaaaagc tgtgtccgat attcttttgg
    11761 agaaatccct tgttttttct taaataaata actgaaatag ttagggtcat tataaccaat
    11821 ttcaaaagca atatctgaaa tacgatagtc tgattgctgc aataattttt ttgctaattc
    11881 gattctttgt tctgtcagat aatcgatgaa tgtttggtta aacgattgag aaaaaatcgt
    11941 actgaaataa gagggactta atgcaacttg ttgagcgacc caagttaagg acatatctgg
    12001 atcggtaaaa ttttctttga taaaatccaa tgcatggttc aaaattggct gatatttgtt
    12061 ccttgaaggc tgtgagggca gagcagttaa gaaagaaatg aacatataaa ctgtcgaacg
    12121 gtaaagcgag tcgtcggcgc tgattgctaa gcgttgactg gtggatgtgc aatgaaccat
    12181 ttcttgcggg attttctttt ctttcaggca gacatgctgg agtagcttag tcaagacaaa
    12241 taaacgatat aatcgtgttc gagttggtgt gccttgtggt ttcgtcaagt gttcgatgta
    12301 cttagatact tcgtttttcg ataatgaaga caaatcaagt gagaggtcaa agtaaaagac
    12361 agaatcaact ggaagtttcg cttcttgaat caaatcctca tagctgataa tttgttcttt
    12421 aagggaactg gcggtttcaa gtatcatctg cgtgatctca aaagatgcag gtaattcgct
    12481 gattctttct gcgacaggac caatggcgac agaaaaatta tttttttgtc ccttttctaa
    12541 ttcatggacc aacgtttggg ctacctgata actggtctcg atcacatttt cttcaagaga
    12601 atcaactatt aaaaatttga tgaatcgaga ggaaacagca gagaagatca cttgttgatt
    12661 ctccctaaat aaaaacttca aataatcact gagatgagcg taatcttcaa agtctgtact
    12721 gacttggttt gctgctaata atacggtcat ttttttccct gctaaagagc gattgaaaga
    12781 ttcagcaagt ttcaatgttt cactgatcgt aagtgagcct ttgaacaaac cgttcaagaa
    12841 atgatttttt ttcaattcga aaacaagatc agaagaagtg tgcgtttctt tttgctgcaa
    12901 atctaaagca gttactgctt tttctattgc ttgtttcaat tcgctacttt taatcggttt
    12961 aagcagataa tcatctgcct gtacctggat tgctcctttc atataatcga aatcgtcgaa
    13021 tccactaata aatatgagac gagtccaagg aagtagtttc cttgcttctt ttgaaaaagt
    13081 caacccgtcc atgaaaggca tttggatatc agtcagaatg atgtcgggct gtaagtcgat
    13141 aattgaagac agtgccatct caccatctcc agcttcgccg acataagtga ttggataata
    13201 agaaccaagg acagttattt gttcccgcaa gttattgcga atgaggtatt cgtcttcgat
    13261 gataaaaact ttatacatgt ttgtttcctc caattgtttt gggagataga gggatcatca
    13321 gtgtgacagt tgttccttct cggtatttgc tgtcaatcgt gataggtttt tgatcatttc
    13381 cgtaataaag aaggagacgc ttgtttacgt tgtaaagtcc gtagccgata ctgaaatctg
    13441 aatcgatccc tttggctaac tctttacgta tttctgccag tttttctgca gggatgccaa
    13501 tcccgttgtc tataactttg aattggatca tctcatcaat tttgcatgtg gtaatacgaa
    13561 taaatccttt gtgacggacg tacttcgttc catggtatac agcattttca atcaatggct
    13621 gcaagatcat ttttaaaata gaaacaggaa aaagtgtttc tggtatatcg atcttatagt
    13681 caagcatatg gccgtatctt atttttaaga tgactagata atcattgata tggctaattt
    13741 ctttttcaag aggaatccag tcttgtcctt tgcttaaaga cgtacgaaaa aagccggaaa
    13801 gtgcatgggt catagtcaga gcttcttcaa agcagtcttg ttcgatcaaa gaaataatgg
    13861 catccaaact gttataaata aaatggggag taatctgagc ttgcaataca cgaacttcac
    13921 tttgtgctaa atggtattgt ttcaaagcgt tctcccgtaa agactggttt agatcttccg
    13981 ccattttatt catgctagcg gtaagtgttt gtaattccag tgtttccggc agggttaaac
    14041 gaaagccaag gttccctgaa gaaagttgtt ctgacatctc gatcagctga ttgatcggtt
    14101 cttggacctt tctctctaaa aagcgacgat tctttttcgc atagaaaaaa atggtactaa
    14161 gaactccaag ttccactatt gttaatagaa tgagcgagcg gacaagtgtc gtattttttt
    14221 tgcttgctaa gtcgatctcc acacgaacaa aatcttgtaa gatatctgac agcagttgag
    14281 tgacagaatc tacctgattg atgatctctt cattggtatc cataaaatct tttgccaaga
    14341 tattattgat cagctcgttt tgataatctt ccagtgtatc gatcgtacgt agcgatacat
    14401 ctaacgtact aatttcttta agtgtatttg tattattttt gatctctcga atatcattcc
    14461 taagttcttc taggatattt tcctcattga actcatctac ggttacttgg ccagaaatca
    14521 agtcccacat actttccaaa actttttgat caaccttcaa agcaattgca tctgcttttt
    14581 ggatatttgc gatcgtctgt tgataaatca aaagctgttg cgaatataac agtatgctca
    14641 gtagcaaagg gataacagca aatagaagca tggctttgtt tgcttgggca aaaatctgtt
    14701 ttatgcttaa attttttgtc atgatacttc tccagtcaat aatttctggt tgggatgtta
    14761 gctatattct gctgggaaaa gacagtctcg gaaataaaaa tatctttagg taatgttttt
    14821 cctgtttgaa tatttccaaa ataacgagta acggtattgg ctacatacca gccgacatca
    14881 ggtttacact cgactacaca attgacgatt cctgagcgta gcttctcgat catatcttct
    14941 tgtccatcaa tagttacaat gatcaaatct tctccaggtt tgattgatgt ttcttttatc
    15001 gcagctaaag caccgtgagt catttcatca ctatggctgt ataatacatc gatttcggat
    15061 aaatcatttg tttctaaata tcgtttcatc tggtcttttc ctttcatccg aataaaatca
    15121 ccgacaattc ggtgaacaat agtcatcgga ggtttatggt cgatggttcc atcttttttt
    15181 agggttggtt tacgattgat tgattccaaa aatccttcgg agcgtagctt ggtcggtgtg
    15241 gagttctctg aaccagcaag ttctaggatt ctgatttcct gtttctttga atttgtgaag
    15301 tgattgctga caaataaacc cgcccgattt ccttctgctt tgaaactggg gccaatgtgg
    15361 gtgacgaaaa gctcgggatc attcgtttga atatgtctgt cgacaagtat gacagggatc
    15421 cctgctcgct ttgcttcttt taatacagag tcccacccgg attcttgcaa aggagtaaaa
    15481 atgatcatat ccactttata ggcaatgaac gtccgtatat cttggatttg ccgttcctga
    15541 ttcatgtagc catttcggta gagaacttgg taatcttctt tttcaagcgc agtgataatt
    15601 gattcagtat gctttttccg ccagctactc tccgttcctg attgcgaaaa accaacgact
    15661 agtgggtcag gtgcttcctt tgtattaggt gtacacccca tcatttggta agcgattaca
    15721 acaagggcta aaaaaaataa aacacgtttc actttaaatc actccatctg gatttgttag
    15781 tactatctta tcagataaaa gggaaaatca aagtcaaaag gaatcaatcg aaaaaaatcc
    15841 aagaaactta aaaaaatcca agacaaaaaa agtacaccta aaaaatttaa agtaaatccc
    15901 ataaaaaaat caatgttaac gctttcttta ttagctatac tcggtgtatc aattgaaatg
    15961 ggggataaaa aatgaaaaac aaatttagaa agattgttgg gactatcgct attttgaccg
    16021 caagcattct attaagtgcg tgtggcaatt caggatctgc agatgatagt acggggtatg
    16081 taggaatcgc tatgccgacc aaatcagcag aaagatggat cgctgatggg aacaacatgg
    16141 tctcggaatt agaaaaactg ggttataaaa cagatcttca atatggagaa gataaagtag
    16201 aaaatcaagt agcacaaata gaaaatatga ttacaaaagg ggtagataca cttgtgattg
    16261 cttctatcga tggttcggct ttaactgatg tgttagctaa agcaaaagaa gcagatatca
    16321 aggtgatcgc ctatgatcgt ttactgatga attcagaaaa tgtcgattat tacgcgactt
    16381 ttgataattt tggagtaggt gtatcgcaag cagcttatat tgaagagcac ttaggattga
    16441 aagaaggaaa aggaccattc acgattgaat tatttggtgg ctcgcctgac gacaacaacg
    16501 cactaatcaa ttacaacgga gtgatgtctg ttttacaacc atatatggac aacggtcagt
    16561 tggtcgtgcc atctggacag acgagtttca gtcaaatcgc tacgcttcgc tgggatggtt
    16621 ctacggcgca agcaaggatg gataatctgt taagcgcaaa ttatactgac caaacattgg
    16681 atgctgtttt gtcgccatat gatccaataa gtttaggaat catttcgtca ttaaaaggcg
    16741 tggggtatgg gagtgaaagt aaacctttac ctgtgattac tggacaagat gcgacagttg
    16801 ctggcgttaa atcgatcatt gccggggaac aaacacaaac gatctttaaa gacacacgta
    16861 tcttagcaaa aaatacgatt gaaatgatca aagcaatcag tgatggagaa gaggttccgg
    16921 tcaatgatac tgaaacttat gataatgggg tgaaaactgt ccctacgtat ttagcaaata
    16981 cagtatctgt cgataaagat aattatcaag cagagctgat cgacaccgat tattataaag
    17041 aatccgatct gaaaaattaa gaggaaccaa acaaggaact aaagagatct aagtcagaga
    17101 gaggataaga attatggcgg attgtatttt aaaaatggca gatatcacca aaaaattttc
    17161 tggaatccga gcgttagatt gcgtgaatct gatgattgaa cgtggggaaa tccatgcatt
    17221 gtgcggagaa aacggagcgg gaaaatcaac attgatgaat gtcttgagcg gcttgtatcc
    17281 atatggctcc tatgacgggg aaatctttta tgatggaaag ctgtgtcaat ttaaagattt
    17341 gaaagatagt gaagaaaaag gcatcgtcat cattcatcaa gaattagcac taagtcctta
    17401 tctttcaata aaagagaata tttttctcgg aaatgaacaa gcagaaagag gcgtgattga
    17461 ttgggaactg acagaaaaga aaacatatga actgctgaat aaagtcggtt tgtctatcga
    17521 cccgaatctt ctagtatccc agattggcgt agggcaacag caactagttg aaatagctaa
    17581 agcattatca aaatccgtcc gtctgttgat tttggatgag cctaccgctg cattgaatga
    17641 agaagaaagc gcgaatttgc tgagattgat ccgagagttg aaagagcaag gtgtaacctc
    17701 catcattatt tcacataagc tgaatgaaat cgtggatatc gcagatcgaa tcaccatttt
    17761 acgggatgga cagacgattg agacactaga aaatgaaatg atcagcgaag agcatattat
    17821 tcgcggaatg gtgggcagag atttagttga tcgttatcct gaaagaaaac cggatattgg
    17881 agaagtttat tttaaagtag aagactggac ggtctatcac cctcttgatc acgagcgaat
    17941 cataaacgac cacatcaact tgcatttgaa aaaaggagag attgtaggaa tagctggact
    18001 gatgggagcg ggcagaactg aattcgctat gagtctattt ggacattctt atggaagcaa
    18061 gatatccgga cgtatattaa aggaaggggc agagatatca gttaagaccg ttccttcagc
    18121 cattgcaaac ggactcgctt atgtctcgga ggaccgaaaa tctttaggac tgaatctgct
    18181 gatggatatt cgggaaaacg tgacgctgag cagtttggat aaaataagtc aaaaaggagt
    18241 attagataaa gaaaaagaag taactgaggc agaaaatttc cggaaaaaga tgcgtataaa
    18301 aacaaactcc atccatcaaa ttgtcagtag tcttagtggt gggaatcaac aaaaagtcgt
    18361 tttatccaag tggctgatga cacaacccga tattcttttt ttggatgagc caacccgtgg
    18421 gatagatgtt ggggcaaaat atgagatcta cacgattatc gaagaaatgg cagcaaatgg
    18481 caaagctgtc tgtcttatct cttctgaact tcctgaaata ttagggatgt gtgatcgaat
    18541 ctatacgatg aacgaaggac gtattacagg agaagtctcg cgagaagatg cagatcagga
    18601 agtcttgatg aaattaatga cgaaagaaga ggtgggttaa gttggaacag gcaaaaaatc
    18661 ttacggataa aaaagagaaa aaactaaatt tgaaaagtag agcagctgat ctttttggtc
    18721 gctacagcat ggtgatcatt ttagcgggac tacttattgc gtttcaattg atgacggatg
    18781 gtattttctg gcgtccattg aatataacta accttgttct gcaaaatagc catatattag
    18841 tactagctgc agggatgtta ttagttgtgc tcttaggtca tgtggatttg tcggtagggt
    18901 cagtgatggc gtttgttggt gcaattgcgg gcatgatgat ggtcaatcgc caaatgaacc
    18961 cgtggatgac agttatcgca tgtcttgcag taggcgggct gatcggcgca tggcaaggtt
    19021 tttgggtagc ttatgcaggg ataccggcgt ttatcgtgac tttagcagga ttgctgatgt
    19081 ttcgtggact gacacaagtt gttctaggag gccaatctct agcacctttt ccagctgttt
    19141 tccaaaaaat gtctacgggt tatttaccag acttaagcga aggaagcagc cttcacttct
    19201 tgacattgat tttaggaatg atcatcacga tcgtgttagt tgttggtcaa tggcgaacaa
    19261 gaaatcgtcg gaaaaatcat ttatttgaag tggaatctcc aacagctttc ttggtaaagg
    19321 cggtatttac agcagtcgtt gtactaggaa taacttatat ctttgcctct tatcaagggt
    19381 atccgattat tttgatcatt cttggtttga tcgttgcagc atatgggttt ttgacaaata
    19441 aaacagtagc aggtcgacag atttatgcga cgggcggaaa tcgaaaagcg gctgaactat
    19501 cagggatcaa aacaaagcga atcacgttct gggtattcgt caacatggga gtcatggcag
    19561 ctttagctgg attagtttta gctgctcgat tgaatgcggc aacacctcaa gcaggtacat
    19621 cgatggaact ggatgcaatg gcggcagtat attttggtgg tgcttcgact tccggcggca
    19681 ttggaacaat catgggtgcg attgtcggtg gcttggtcat gggtgtattg aacaatggca
    19741 tgtcgatttt aggcgtcggg gtagactggc agcaggcaat caaaggattg atcttgcttt
    19801 tagccgttgt gctagacttg tataataaga aaaaacgaac aacgtaggag aacgccggtt
    19861 attcagaaaa aagactgtga caagacattt gtcacagtct ttttttcgct aaaagcgaac
    19921 attcattgtt tcgtttgtat agactaaaaa taatatatgt ttttttacca ttcgtataaa
    19981 ttctcttttt gctcttgatt tttttgctta attgagttaa attacatagg gcgctaaaaa
    20041 aagccttgat taaattaata atgttcgtgt tttgctggat gtgaacatca ggaggataat
    20101 gtaatgaaag tatttgatta cgaagatatt caattaattc ctaataaatg tatcgtaaat
    20161 agccgttctg aatgtgatac aacggtcaca ttaggtaaac atacattcaa aatgccagta
    20221 gtgcctgcca acatgcagac gattattgat gaaacaatcg cagaattctt agcggaaaat
    20281 ggttatttct atatcatgca tcggtttgat gaagcagctc gaattccgtt tatcaaaaaa
    20341 atgaaaaagc gtggattgat tacctcgatc agtgttggcg tcaagaaaga ggaatattcc
    20401 tttatagaga agttggcaga agaatcgcta aatcctgatt atatcactat cgacatcgcg
    20461 catggacatg ctaactcagt tattgatatg attcagcata ttaaaaaata tttgcctgaa
    20521 acttttgtga ttgccggtaa tgttggtaca ccggaagccg tccgtgaatt ggaaaatgcg
    20581 ggcgcagatg caacaaaagt cgggattggt ccaggaaaag tatgtattac aaaaatcaag
    20641 acaggctttg ggactggagg ctggcaactg gcagctctta gatggtgtgc gaaagcagct
    20701 cgcaagccga tcatcgctga cggtgggatc agaacacatg gcgatattgc aaaatctgtc
    20761 cgttttggtg cgacaatggt catgattggt tcattgtttg ccggtcacga ggaatctcct
    20821 ggcgaaacaa aagtcgaaaa tggaatcgtg tataaagaat actttggttc tgcttcagaa
    20881 ttccaaaaag gcgaaaaacg aaatgttgaa ggaaagaaaa tctggatcca acacaaagga
    20941 tctttgaaag atacactagt ggaaatgcaa caagaccttc aatcatctat ttcttatgct
    21001 ggaggaagag atctggaagc cattcgaaaa gttgactatg tcatcgtgaa aaattcgatt
    21061 tttaatggag acacaatcta aaaaataaat aaaatattag gatgttgtga aaaagttgtc
    21121 ctgcattaag taataagaac agccaaacaa aaatagttct ttatatgttt tgtttggctg
    21181 gaattattat cgcagataca agcttttgaa catcattatt cagaataagg gactgtgata
    21241 agatttttgt cacagtccct tatgttttgc aaaaaaagtt tttctttttt ctcgtaaatt
    21301 cggtaaaatc aaaagaaaaa atagacaata gaaaagagaa gcttatgtgg gaaaaagtag
    21361 agaaaaaaca aggaccgatt tatcgtcaaa ttatggaaca gattatgaga gaaatcgaaa
    21421 acggaaggat caatcctgga agtcaacttg aatcagaacg aaaactaggg caactttttg
    21481 gtgtgaatcg ttccaccgtt gtccatgcat tggaagagct tagggagctt ggggtactaa
    21541 cgagcaaacg tggcagcgga tggtttgtta atcagactga atgggggaaa ttcgctgttc
    21601 caagaatcga ttggcggcaa atgatctctc ctcgttatga gcagacggac tggtatgagc
    21661 aaaaaataag agaagcaaag cagatccaaa aaacatcgtt tcttgattta tacgctagcg
    21721 agatgccaga tgagcttttg ccgaattttg agtttccttc ttattctctt gaagaaatct
    21781 tagcggaaga aaaagaaatg gatcttttag gctatactcc gttacgtaac aaaatcaaac
    21841 ggtatttgga aagtaaattt gaattggcat tttcccccaa ccagcttctt gtgactggtg
    21901 gcggtcagca agcaatcttt ttgattttac aaacgattct ttcagctgga gaggggatag
    21961 ctgtagagtc accttctttt ttttaccgat tacctctttt caaagcttca ggaacgcgtt
    22021 tatttgggat accgatggat gaagaaggga tcgacttaga aaaactgaaa gaatcgatcc
    22081 ataaaaataa attgaaagca gtgcttatca acccgaattt tcaaaaccct acaggaaaag
    22141 tgatgagtca aaaacgacga gaaaagctag tagaattgtg tcgacagtat cggcttccaa
    22201 ttattgaaga cgatgtattt acggatcttt catttctaaa ccaagaagaa gtccttccta
    22261 tacgaacgat tgatccggat aatgtgttat acgtcggttc gttatctagg gttttaggaa
    22321 aaacaacgaa aatcggttgg gtgataggac ctgagacttt tatctcgcaa ctagctcaag
    22381 cgcaagaaat gatggagttt tcgatgaata tccttacaca aatcacagtg gctaatgtat
    22441 ttaatgaagc aattgatttg aaaatgcaag atgtaagaaa aaagttgaaa gaaaaaagcc
    22501 gactgataag cgcttgggga gattcacaat cgtttttcaa gcttcattca attttaggtg
    22561 gttactatgc ctggattact tgggatggga aaaaactaga ccccgaacta gcagaaaaag
    22621 tagtagatat gggtcttgga attgcaccaa gtctactttt tgggcaagat ataaatggaa
    22681 tcagaatcaa ttacagtagg ctggatgaaa accaaatacc gttgttttat tcaaagatga
    22741 aaattctaga aaagtggtta acagaataat ttcacaaagt aaatagatta aagaatataa
    22801 cagcttggat ttaaatttca agctgtttct tttttttcgg aaaaagaata cgatataaag
    22861 aaactaagaa aaaaccatta tttgtacgtc cgttcacgat gtgtaaataa aataaatgtt
    22921 tgcaacaaaa ctgtataaca ccaacgtttt taaatagaaa cagatggttg taagtttgat
    22981 tagaaaatag aaaaaatgca gatgaaatat aaaacgcttt ccttattgtg attttattca
    23041 ctaatgctgt taatatgcgg attggcataa tttcctttca tcgtgaaaat ttgtgctttt
    23101 agcttgtatt tctcataaat ccatgataat ttattaatga agacggttaa atagtgatac
    23161 agttttattc actgtttcaa tcgcagaatg aagagatttt cttatagatg agagaggaat
    23221 gtgtgacaaa tggcaaacaa aaaagcaccg atcgacttcc aagcactgct agatgaagtc
    23281 aatgcagatt tcccagtata tcaagcactt gatcaagatg gaaacgtagt taacaaagat
    23341 ttagtacctg atttatccga tgatgaactc gttgaattaa tgactcatat ggtatggtct
    23401 cgtgtattag accaacgttc aacagcgctg aaccgccaag gacgcctagg cttttttgca
    23461 ccaactgctg gacaagaggc aagtcagctg gctagtgctt acgcatttga taaagaagat
    23521 gttcttcttc caggttatcg ggatgtacct caactagtaa aacatggttt acctttatct
    23581 caagcttttt tatggtctcg aggacatgct gcaggtaatt cttatccgga agaattgaaa
    23641 gctctaccac cacaaattat catcggtgca caatatgtac aagcagccgg tgttgcatta
    23701 ggtttgaaga aacgaaacaa gaaaaatgtc gtcttcactt atactggtga tggcggttca
    23761 tcacaaggtg atttttacga agcaatcaat ttcgctggcg cttaccatgc aaatgccgta
    23821 ttttatatcc aaaacaacgg gtatgcgatc tctacacctc gtgaaaaaca aactgcagca
    23881 aaaacacttg cacaaaaagc tgtcgcagct gggattcctg gtattcaagt cgatggaatg
    23941 gatccgcttg cagtatatac tgtatctaaa atggcacggg aatgggcgat cagtggaaat
    24001 ggaccagtcc taatcgaaac actgacttat cgttatggac cacatacact atctggtgac
    24061 gatccaaccc gctaccgtac aaaagacaca gatgacgatt ggcagaaaaa agatccattg
    24121 atccgcttcc gtaaatattt gacggaaaaa ggactttggt ctgaagaaaa agaagaacaa
    24181 gtcatcgaag caactaaaga agaaatcaaa gcagctattg ctgaagccga caaagtacca
    24241 aaacaaaagg tttcagattt cttgaaaaac atgttcgaag tatctccaca atcaatcaaa
    24301 gaacaaattg caatttatga agcgaaggag tcgaaataat catggcacaa aaaacaatga
    24361 tccaagcaat cacagatgcc ttagcacttg aactagaaaa tgacgagaat gtcgttgttt
    24421 ttggtgaaga tgttgggaaa aacggaggag ttttccgggc aactgaagga ttgcaggaaa
    24481 agttcggaga agaccgagta ttcgatactc ctttagctga atctggaatc gcaggattga
    24541 gtttcggtct tgcgttagaa ggatttcgtc cagttcctga aatccaattc tttggtttta
    24601 ttttcgaagc aatggacgaa gtagtagctc aaatggctcg tacaagatat cgaatgagcg
    24661 gcactagaaa tctacccatc acaattcgtt cgccatttgg tggcggtgtt catacaccag
    24721 aattgcattc ggataattta gaaggtttga tcgctcagtc tcctggtatt cgagtagtga
    24781 ttccttccaa cccttatgat gcaaaaggac tattgatttc ttctattaga agtaacgatc
    24841 cggttgtttt cctagaacac atgaaactat atcgctcatt ccgagaagaa gtgccggatg
    24901 aagcttacga agttcctttg gataaagcag cagttactcg tgagggaaca gatgtatcta
    24961 ttatcacata cggagcaatg gtgcgtgaag caatcaaagc agcagacaat cttgcaaaag
    25021 aaaatatatc tgttgaaatc attgatcttc gtacagttgc gccattagat gtagaaacga
    25081 tcattcaatc cgtggaaaaa actggtcgcg tagttgttgt ccaagaagca caacgacaag
    25141 cgggcgttgc cgcgcaagta gtttctgaga tctcagaacg cgctatcctt tcattagaag
    25201 ccccaatcgg acgagtttct gcacctgata cagtcttccc atttggacaa gcagaaaatg
    25261 tctggttgcc aaatgcaaaa gatatcgaag acaaagtaaa agaaatcgcg gaattttaag
    25321 aaaagaaggg acgaaacaaa gtcatggctt atcaatttaa attgccggat atcggtgaag
    25381 gaatcgcaga aggtgaaatc gtcaaatggt tcgtcaaacc aggagatacg atcaatgaag
    25441 atgatacatt actagaagtt caaaatgaca aatcagtaga agaaatcccg tcacctgtta
    25501 caggaacggt taaaaatgtg attgttccag aaggtaccgt agcaaatgtt ggggatgtat
    25561 tagtagaaat cgatgcacca ggacacgaag acaatgaagg ggacagtggt gtagccgcag
    25621 aatcccaaac tccagctaaa ccggctgctg aacccactgt tgatacagaa tcagctggat
    25681 cgtcaagcga aggagtcttc caatttaagt taccggatat cggtgaagga atcgcagaag
    25741 gtgaaatcgt caaatggttc gtcaaaccag gagatacgat caatgaagat gatacattac
    25801 tagaagttca aaatgacaaa tcagtagaag aaattccgtc acctgtcaca gggacagtta
    25861 aaaacgtgat tgttccagaa ggcactgtag caaatgttgg ggatgtatta gtagaaatcg
    25921 atgctcctgg acacaatagt gcaccgtcaa ctagtgcgcc atcagctgaa gcaccaaaag
    25981 aaaaagtgga aacatcaggt tcagcaagtg tcgttgaagc tgccgatcca aataaacgtg
    26041 tattggctat gccatctgtt cgtcaatttg cccgtgaaaa agatgtagat atctcacaag
    26101 taactgcaac tggaaaaggt ggcagagtaa caaaagagga catcgaaaac ttcttggcag
    26161 gtggtccaag cagtgcacca gcgaaatcag aagcgccaga agcggcagca ccgaaagaag
    26221 cagctcctgc agcagaaagc aaaccagctg caccagcaaa accattcaag tccaacttag
    26281 gagacttgga agaacgtgta gcaatgacgc caacacgtaa agctatcgct aaagcaatgg
    26341 tcaacagtaa gcatacagct ccacacgtga cattgcatga tgaagtagaa gtgtctaaac
    26401 tatgggataa tcggaaacga tttaaagaag ttgctgctgc aaatggtacg aaacttactt
    26461 tcttgccata tgtcgtgaaa gctttaacag cgactgttaa gaaataccca gtattgaatg
    26521 catctatcga cgatgcaaac caagaaatcg tttataaaca ttactacaat atcggtattg
    26581 caactgatac ggatcacgga ctatatgtac caaatgtgaa agatgcagac cgcaaaggaa
    26641 tgtttgctat tgcagacgaa atcaatgaga aagcaaaatt agctcatgac ggcaaactat
    26701 ctgcagaaga tatgcgcaat ggaacaatta cgatcagcaa tatcggatct gtaggtggcg
    26761 gttggtttac accagtcatc aactatccag aagtagctat tttaggtgta ggaacgattg
    26821 cacaacaacc aatcgtaaat gctgaaggcg aaatcgttgt cggtcgtgtg atgaaactgt
    26881 cattaagttt cgatcaccgt atcgtagatg gagcaactgc gcaacaagca atgaataata
    26941 tcaaacgtct tttagctgat ccagagctat taatgatgga aggatgataa acaaatggta
    27001 gttggagatt ttgctgtaga attagataca gttgtaattg gtgcgggacc tggtggctat
    27061 gtagctgcca tccgcgctgc tgaaatggga caaaaagtag caatcattga aagagagtat
    27121 attggcggtg tttgtttgaa tgtcggctgt attccttcaa aagcactgat agctgcagga
    27181 caccattatc aagagtcact agattctgaa atgtttggag taactagtga aaacgtcaaa
    27241 ctagattttg caaaaacaca agaatggaaa gaaaacaaag ttgtccatac gttgacttct
    27301 ggtgttggtt tcctattaaa aaaacataag gttgaaacaa tcgaaggaga agcgttcttc
    27361 gtagatgatc acacactacg cgtcattcat ccagattcag ctcaaactta ttcgttcaat
    27421 aacgcaatca tcgcaacagg ttcccgtcca atcgagattc caggatttaa attcggagga
    27481 cgtgtgttag attctactgg cggattagca ttgaaagaag tacctaagaa atttgtgatc
    27541 atcggtggcg gtgtgattgg tgccgaattg ggcggagcgt acgcaaactt aggagcagaa
    27601 gtaacgattc ttgaaggctc accacaaatt ttaccaactt acgaaaaaga tctagtaaaa
    27661 ttagtagaag atgatttcaa gaaaaaaggc gtaacagtcg taacgaatgc aatggcgaaa
    27721 gaagcagtcg ataacggtga cagcgtgaca gtgaaatatg cagtggacgg gaaagaagaa
    27781 tccgttactg ctgattatgt gatggtcact gtaggtcgtc gtccgaatac agatgatatg
    27841 ggcttagaac aagcgggtgt cgaagtagga gaacgaggat tgatcacggt ggataaacaa
    27901 ggacgtacga acgtacctaa catctatgcc atcggtgata tcgttccagg ggcggcatta
    27961 gcacacaaag ccagttatga agcaaaaatc gctgctgaag caatctctgg caaaaaagta
    28021 gctgtagatt ataaagcaat gccggcagta gccttcactg atcctgaatt ggcttctgta
    28081 ggtatgacga tcaaagaagc aaaagatgct ggtatcgaag caaaagcata taaattccca
    28141 ttttcaggga acggtcgggc attatcgctt ggtaaaacag aaggattcat ccgtctagtt
    28201 acgacgatcg aagataacgt gcttattggc gcgcaaatcg gtggagtcgg cgcaagtgat
    28261 atggtctctg aattagcttt agcaatcgaa tcaggaatga atgctgaaga tatcgccttg
    28321 acgattcatc ctcatccatc tcttggcgaa atcgtcatgg atgcttcgga attagcatta
    28381 ggtttaccaa tccatatttg atttgaataa tacaggcgaa acgcaactga aaaatcagtt
    28441 gcgttttgtt ttttagaatt atcaataata ttattatgta aacttaaaaa tatgacaact
    28501 tcttggttta ttaccacttc ctatgctaca ctgaagagga atatgaaata ggagagttga
    28561 agatgcaaga atatcagtat ccacttgatt tagattggac aacagacgaa atggtcattg
    28621 taatgaagat gtgggaagat ctggaacgtg cgaatgaaaa agggataaac aatgaagctt
    28681 ttttgaagac ttatcagcag tttaaaactg ttgtcaaatc cattggtgag gaaaaacgat
    28741 tgggacggga atttgaaaaa gcttccggtt attctttata tcgtacagtg cagcaagcaa
    28801 aaaaaaatcc aaacaaaact ttgagaatga acgggtgaga taaatggaac agatgattga
    28861 tcatatcaag tcctggcttg aagaagctgg tgagaaaatc aaatcttctc aacaaacatt
    28921 aacaatctcg caaaaatcaa atcgaacgga tctagtaaca aatgtcgatc gaaacatcca
    28981 gaattttcta atcgaaaaaa tccattcctt tgatccagag gcgaaaatat tggcagaaga
    29041 agaagggtgt aatcaattat cggatctcga tggaagagtc tttatcatcg atcctattga
    29101 tggcaccttg aattttgtga ttgaagggga aaatttttgt attatgctcg ctgtttatga
    29161 atccggcgtt ggaaagcttg gtttcattta tgacgtgatg agaaatgaac ttttttgggg
    29221 tggaaaagga ataggcgtgt ataaaaatga tgagccattg cctagaccag caaataaatc
    29281 cttatcggaa ggcttacttg gtgtgaatag ttatcttttc ggtcataata aatatggaat
    29341 ccgagaaatt gcggaaagca gcatggggac acgtatgtac ggatgcgctg gtcttgaact
    29401 agtggcaatc ttgaaaggaa accacattgc ttatatttca aaccttagcc cttgggatta
    29461 tgcagcggga aatgtcttat tggaagagtt tggtatggta acaagcggtc tttttggtac
    29521 tcctttgaca tttgaaaaaa gggaatacta tttagcaggg acttcttgtg catatgcaga
    29581 aatcgcagac cttttgaaaa aagaaggctg aaaaaaaggt agaaatcatt cacaaaaatg
    29641 ataaaaacga gcaaaagatt aattttctcg aaaaaatgaa aacattttac tatatttcat
    29701 gaaaaaagtc tgctataatt agcagtcgag taaaatttgc cctgtcataa agaaccaata
    29761 aaaaaggagt tagttacatt gaattataga aacgatatcc gtaacgtcgc aattattgcc
    29821 cacgttgacc acggaaaaac aacattagtt gatgaactat taaaacaatc agatacatta
    29881 gacgcacata cgcaattgca agaacgtgcg atggactcaa atgcgctaga aaaagaacgt
    29941 ggtatcacga ttttagccaa aaacacagct gtagactata aaggtattcg cgtaaacatc
    30001 atggatactc caggacacgc ggacttcggt ggcgaagtag aacggatcat gaaaatggtt
    30061 gacggcgttg tgttggtcgt agatgcgtat gaaggaacaa tgcctcaaac acgttttgta
    30121 ttgaaaaaag cactggaaca acatatcaca ccaatcgttg tcgtaaacaa gattgacaaa
    30181 ccttctgctc gtcctgagca tgtcgtagat gaagttcttg aactattcat cgaattagga
    30241 gcagatgatg atcagttaga ctttcctgtg atttatgcat ctgcactaaa tggtacgtca
    30301 agcttgtcag atgatccagc agatcaagaa ccaaccatgg cacctatttt tgatacaatt
    30361 attgaaaaaa tccctgctcc tgtcgataac agcgacgaac cattacaatt ccaagtttca
    30421 ttacttgact acaacgatta tgtaggtcgt atcggaatcg gacgtgtatt tagaggaaca
    30481 atcaaagtag gagaccaagt agcattaatt aaattagacg gaacagttaa gaaattccgt
    30541 gtaacgaaat tatttggttt ctttggacta aaacgtctag agattcaaga agcaaaagct
    30601 ggagacttga ttgctgtctc aggtatggaa gatatctttg tcggtgaaac agttactcct
    30661 gtggatcatc aagatgcgtt acctatcctt cacattgacg aaccaacatt gcaaatgact
    30721 ttcttagtta ataattcacc atttgccggt cgagaaggta aattcgtaac cgctcgtaag
    30781 attgaagaac gtttgatggc agaacttcaa acagatgtat ccttacgagt tgaaccaaca
    30841 aactctccag atgcatggac agtttctggt cgtggagaat tgcatttgtc tatcttgatt
    30901 gaaaacatgc gccgtgaggg atacgaattg caagtatctc gtccagaagt tattgaaaaa
    30961 gaaatcgacg gtgtaaaatg cgaaccgttt gaacgcgtgc aaatcgatac accagaagaa
    31021 tatatgggta gtgttatcga gtccttgagc ttacgtaaag gtgaaatgca agatatggtc
    31081 catacaggaa acggacaaat ccgtttgaca ttcttaacac ctgcccgcgg tttgattggt
    31141 tattcaactg aattcctttc aatgacacgt ggttacggaa tcatgaacca tacttttgac
    31201 caatatctgc caatgcttcc tggtcaaatt ggcggacgtc accaaggggc gcttgtttcg
    31261 atcgatacag gaaaagcaac gacttattct atcatgagta tcgaagaacg cggtacagtc
    31321 ttcgtagaac caggtacaga agtatatgaa ggaatgatta tcggggaaaa cagccgtgat
    31381 aatgatttga ctgtcaacat cacgaaagca aaacaaatga caaacgtacg gtctgctact
    31441 aaagaccaaa catctgtcat caaaaaacca aaacaattga cgttggaaga atctttggaa
    31501 ttcttgaatg atgatgaata ttgcgaagta acaccagaaa gcattcgctt gagaaaacaa
    31561 attttagaga aaaatgctcg tgaaaaagca tctaaaaaga aaaaataatc tgtaaaagga
    31621 tgaagagaga aaccagcttg ttataaaaaa tagctggttt ctttttttgt ccaactcttt
    31681 tctgtggaga agtcatcttt gtcaatggac ataaattcag aaaatacgcc gaaagacgtt
    31741 taaaagactt ttaaaaaact tatttctaga gagtttttgt ttcataataa gataatgttt
    31801 cacgaaagga gatcaacttg tccttcgaag aaaatcgaag taaaatggca agagaaagga
    31861 gcgggaagat gaacgactta ttaaaacaat actttggcta tgatgagttt agacctggtc
    31921 aaaaagaaat tatccagaaa gtcattgatc aggaaaatgt attaggaatc atgcctaccg
    31981 gaagtgggaa atcaatctgt taccagcttc cagctttact attagatggg cttactgtgg
    32041 ttgtgtcccc tttaatctct ttgatgaagg atcaggtgga cgcggcgaat caattaggta
    32101 tacccgccac ttttatcaat agctcgttag atggttacga aacagctaga cgcttccaag
    32161 aaatcgatag acagcagtac cgtttacttt atatcgctcc tgaacgtttt atcatgccgg
    32221 attttatcca agcaatgaag cgatggaacg tccgtatgat cgctattgac gaggcacatt
    32281 gtatctctca gtgggggcat gatttccgcc caagttattt acagatggcg aaccagctag
    32341 atcaactgcc aaatcgtcct gtaattgttg ctttgacagc tacagcgact gtgcaagtag
    32401 ctgctgacat aaaaagactt ttaaaaattc cagaaaacaa ccatattcag actggttttg
    32461 aacgagaaaa tctacgattt caagtaatta aagatcaaaa aaaagaacaa tatctaatcg
    32521 aatatttaaa gatcaacaaa aatcaatctg gtatcattta tgcagcaacg agaaaagaag
    32581 tagaccgtct ctatcatttg cttaaaaaat tcgatttttc cgtgggtaga tatcatggtg
    32641 gtctaaatga gaatgagcga acagaaatgc aagaagcctt tttatacgat cgcttgcagc
    32701 tgattgtagc gacaaatgca tttggtatgg gtataaataa aagcaatgtc cgttttgtta
    32761 tccattatca aattcctggc tctctagaag catactatca ggaagctggt cgggcgggaa
    32821 gagatggtct ttccagtgaa gccatcctgc ttttctctcc tcaagatatc caagtccaaa
    32881 agttttttat ccagcagtcc caaagagaag aagggcaaaa gcaaaaagaa tatgaaaaat
    32941 taaaagcgat gacagaatac gtgtatattg agtcctgtct tcagcaatac atcctaaact
    33001 attttggcga gacaagcagt ccgtgcaacc gttgtggtaa ctgtttagat gacagagaaa
    33061 tagtagaagt aacgacagaa gcacagatgg ttctttcttg cttaaaacgt atgggtgaga
    33121 actatggtaa acaaatgctg atgaaagtct tagcaggatc gaaagaacaa aagctgcaag
    33181 ctttaggttt tggacattta tctacctatg gactgatgaa aaaccaatcg caaaaagaaa
    33241 cgatgcagtt aattgaatat ttgatatcga atggctatct tctgaccatc aatggtgagt
    33301 atcctgtttt gaaagtaaca gaaagaggga tacaagtatt gaaaggacaa gaatctgttt
    33361 atcgaaagga acccaagaaa gtccagcagc tatcggatga agaaaccgat acactatttg
    33421 aagtgctgcg ggaactgcga acggatctag catcagaagc aggcgttccc ccgtatgtcg
    33481 tattttcaga ttccacattg aaagaaatga gccgaatacg tccatcaagc agattggaga
    33541 tgcttcagat aaaaggagtt ggccaaagca agcttgataa atatggcgaa gcctttttat
    33601 ctcggataaa aaatgcggac tctaacgttc tggataagta gaaagaacga atacgtaaaa
    33661 atgaataaaa acaactggtt aaatctgttt tttttgctcc ttatgaaaat caaatatgtt
    33721 ataattaagg aattagaatg ggaggtaagc acagatggat tctttgtcga aaaaacttgc
    33781 gatccaagta ttaaatgatg aagcagaacg gatcaaacga ttaatccgaa atcaaaacaa
    33841 cagtctctgt atctcacaat gcaaagcctt cgaagaagtg gtagatacac agatgtacgg
    33901 cttttcccgg caagtcggtt ttgctgtccg tataggagta atcggaaaga acgagggcca
    33961 gctgatgtta agtgagctag aacgagaatt gaatcagtta tatagtgagg tttaccaaga
    34021 aatttatgat aaaaaagaag ttggcaagga ggaatagact tgcctaagaa agtgaaaaaa
    34081 aggcatctat tagactatag catcttgatt ccctatttaa ttttatgtgt attgggattg
    34141 atcatggtat atagttctac gtcgtatcta ttattagaaa acggacaaaa tccgtccgca
    34201 tcagtcatta accagagtat tttttgggtg cttagcttga ttgctatcgc actactttat
    34261 aaaatgaaaa cagatgtttt gaaaaaccag cgtttgatca tggcagccat cgcagttttg
    34321 acgattttgc tgctgattgt tgtgtttttt ggtaaggaga tcaatggggc gaaaggttgg
    34381 ctgcaaattg caggattctc gatccaacct gccgaatacc taaaaatcat ttctatctgg
    34441 tacctgtctc tgactttatc taaaagacaa aacagtgtac aaaaagattt tcttgggact
    34501 gtgaaacgtc cacttgcaat ggttatcgga cttactgcat tagtcgctat tcttcccgat
    34561 tttggtaatg cggcagttat ctttttgatt attttggttc tgcttttagc cagcggagtc
    34621 aattatgtgt atactttgat tgtaggagta ggaggctttt gtctaagtac ttttacgatc
    34681 tggctgatca atatcacaaa cggaaagatc ttacccggac gtttacagta tatctataat
    34741 cgatttgcta tttaccaaaa tcctttctca gacgaattaa ataaagggca tcaactagtc
    34801 aacggctact atgccatgtt caacggagga ctatttggca ggggattagg taacagtatc
    34861 cagaaaaaag gatttttaca agaagcacaa accgatttta tttatgcaat cgtagtggaa
    34921 gaactgggag tgattatggg gattttgata ttagcgttgc tattctttat gattgtgcgc
    34981 attgtccttg taggtatccg ttcaaaagat ccgttcaatt cgcttttgtg cattgggatc
    35041 ggcgcaatgt ttttgatcca agtatttgtc aatcttggag gaattactgg cgttattcct
    35101 ttgacaggga tcacgttccc atttttaagt caaggaggct ccagtttgct gatgctttcg
    35161 atttgtgtcg gctttgtcct caacatcagt gcggatgaaa aacgcaagag cttaggttta
    35221 taataaaaaa agatgtagga gtggttcgat gaaaaaagtt ttagttgcta acaggggtga
    35281 aatcgctgta cgtgtatttc gtgcttgtac agaattagga attaaaactg ttggaatcta
    35341 tgcagaagaa gatgaatatt ccgtacaccg tttcaaagcg gatgaagcat atttagtagg
    35401 acaaggaaaa aaaccgattg atgcgtatct agatattgaa gggatcattt ctatcgcgaa
    35461 agaatgcgga gctgacgcta ttcatccggg ttacggtctt ttgtcggaaa acttgaattt
    35521 tgcgcaacgg tgcgaagaag aaggcatcat ttttgttgga cccaaactgc atcatttaga
    35581 tatttttgga gataaaatca aagcaaaagc tgcagctatc gaagcgggga ttgcttcgat
    35641 tccaggcaca gatggaccaa tcgcttctat agacgatgcc ttggagtttg cgaaacaata
    35701 cggttatccg attatgatca aggcagctct aggcggcggc ggacgcggca tgcgtgtagc
    35761 tcacgatgaa aagagtgcaa gagagggcta cgaacgtgcg aaaagcgaag caaaagcagc
    35821 ctttggtagt gatgaagtgt atgttgaaaa atacatcgct aatcctaagc atatcgaagt
    35881 acagattctc ggggatacgc atggaaacgt catccatctt tttgaaagag attgttctgt
    35941 tcagcgccgt caccaaaaag tagtagaagt agcgccttgt gtctctatga atgaacagca
    36001 acgtcaaaaa atctgccagg cagctgtcca attgatgaaa tatgtcgggt atgtcaacgc
    36061 aggtacagta gaatttcttg ttgaaggaga tgatttttac tttatcgaag tcaaccctcg
    36121 cgtccaagtg gaacatacga tcacagaaat gattacagat attgatatcg taacaacgca
    36181 actgttgatt gctcaaggtt tggatctgca taaagaaatc ggattgcctc agcaagaagg
    36241 aattaaatta aatggttcag ccattcaatg ccgaatcact acagaagacc cgttgaataa
    36301 ctttttacca gacacaggga aaatcgatac gtatcgttca cctggtggtt ttggcgtacg
    36361 tctggatgtc ggaaatgctt acgcgggcta tgtagtgaca ccgtattttg attcattgct
    36421 agtaaaagta tgtacgcatg gtgctacctt tgaaacagcc attcagaaaa tggaacgttg
    36481 cttgaaagaa tttagaatcc gtggagtgaa aacaaacatt ccatttatgc tcaatgtcat
    36541 cacacatcct gaattccaat caggaaatgc aaaaacaacc ttcattgata gtacagcaac
    36601 attatttgaa tttcctcgac tacgagaccg cggaaacaaa acgatgaaat atatcggtga
    36661 aattactgtg aatggttttc caggtatcga aagtggcgaa aaaccatttt atgaagaacc
    36721 acgtatgcct aaagatctaa tcacaagaac tgattacgta acggctaaga atgtattaga
    36781 tgcgaagggt gccgatgctt tggtcgaatg gatcaaagga caggaaaatt tgttgttgac
    36841 ggatacgaca tttagagacg cccatcaaag tctgctagca actcgtgttc ggacaaagga
    36901 tttcaagcag attgcacgtt tgactggaga aggtcttcct gaactctttt caagtgagat
    36961 gtggggggga gcaacatttg atgtggctta ccgctttttg aatgaagatc cttggcaacg
    37021 gttgagaaaa attcgttctt taatgcctaa tacgttgctg caaatgcttt tcagaggttc
    37081 aaatgccgtt gggtattcta attatcctga caatgtattg gtggaatttg tcaaagaagc
    37141 agcagcgcaa ggaatcgatg tatttcgaat ctttgatagt ttgaactgga ctccacaaat
    37201 ggaaaaaagt atccaagctg tacgggatac tggtaaaatc gcagaggcag ctatttgcta
    37261 tactggcgat atcaacgatc caagtcgggc aaaatacaat gttcagtatt ataaagacat
    37321 ggcaaaggaa ctagaacaac tgggtgcgca tatcattgca atcaaagata tggctgggct
    37381 gcttaaaccg caggcagctt atcgtttgat tagtgaattg aaagaaacga cagatctgcc
    37441 aatccatctg catactcatg ataccagcgg gaatggaatc atcacttatt ctgctgcttc
    37501 aaaagcagga gtagacatcg ttgacgtggc aatgagtgca atgagtggga atactagtca
    37561 gcctagcatg agcagcttgt attatgcact ggtcaatggt ccacggctgc cagaaatcac
    37621 gattgaaaat gcacagaaat taaaccatta ttgggaagat gtgcgcatgt attacaaacc
    37681 gtttgaaaac ggcttgaatg ctcctgaaac agaagtatat atgcatgaaa tgccaggagg
    37741 acagtattcg aatctacagc aacaggcaaa agctgtcgga ttaggtcatc gctgggatga
    37801 aatcaaacaa atgtatcata ctgtcaactt gatgtttggc gatatcgtta aagtaactcc
    37861 atcatctaaa gtagttggag atatggcact atttatggtc caaaatgatc ttacagaaga
    37921 agatatttat gaaaaaggcg aaacactaag tttccctgaa tctgttgtga ctttcttcca
    37981 aggagaacta gggcaacccg ttggcggatt tcctaaaaaa ttgcaaaaga ttattcttaa
    38041 aggcagacct gcattgaatg aacgtccagg tcttttggct gaatcagtcg acttcaacga
    38101 agtaaaaaaa gaattggcag aaaaaattgg ctatgaacca aaacaagacg aagtattaag
    38161 ctatttgatg tatccgcaag tctttttgga ctatcaaaaa gcatataatc aatttggcga
    38221 tgttacatta ctggacacac caacattttt ccaaggtata cgtttgggtg aaacaatcaa
    38281 tgtacaaatc gaacgcggaa aaacgcttat cattcgttta gatgaaatcg gagaacccga
    38341 tatcgaaggg aatcgcgtac tgttcttcaa tttgaatgga caaagacgtg aaatcactat
    38401 caaagataat tcgatcatca gtacggtcca aacccgattg aaagctgaac cgacaaaccg
    38461 agaacaaatt ggagcaacga tgtctggttc tgttttggat gtattagtga aaaaaggcga
    38521 taacgttaaa aaaggtgata cgttgatgat tactgaagcg atgaaaatgg aaacggccat
    38581 cgaagcacgt tttgacggag aagtcgcaca tgtatatgtg tcttcaggag acactatttc
    38641 gtctggtgac cttttgattg aagtgactga gaaataataa gcgaaaggtg gggaaacatg
    38701 aaaagactgg gtttgttttg gctgattctg ctattagtta ttgtggcatt ctacatggaa
    38761 ccagtcttgt ttccgccaaa acttgaagtg aaggaaaact ctgatcaaca aatatcaaac
    38821 caaatgacaa cgtggaaata ccaggagctt aaagcaactg gtttttctac ttatatcgga
    38881 aaaccgatcg atcagctgga aaaggatttt ggccaagcct atgatcgatt aactagcgga
    38941 tttggttttg aaacaagata ttatgaaaat aaagctagtc atctcacatt tgaagcgaac
    39001 atccaagaca gcagagtcac aactgttaaa gtactgcaaa cagggcctgc tgatatcgcg
    39061 ccgttttatt tagggatgac gatgcaagat cttacgaaaa tcactactat ctatactaat
    39121 ttcacttttg agtaccatga tacagaagtt ggaatcgaac taatggaaga ggatatgaat
    39181 tatcgtccgc taattgcttt tgataacgac acttttgcta ttttattttt tgatcaaaca
    39241 acaaacggcc tattttcagt tgtgtattta gataaagata gtcttttgaa actattgcca
    39301 tatcaagtgt ttggtgaagg ccttccacat tatcaagcag ataaatcggc tgattgggaa
    39361 tcaatcaatc gcgttaaaga acgaaaaagt acgactttat taaatatgct tagaagagaa
    39421 gacgaacttc ccatatacaa tcaaacactt gcctttcaaa accacagtca gctgctacta
    39481 catgattttt tgacaaggcc tgaggaaata ttgtcagagg aacgtatggc tgattggcaa
    39541 aaatcgctag gcagtgctca aacgacagtg aaattctcac tgacgaatga tgaattggag
    39601 aagttaatca gtaaacaaaa attagaaaat actagtgggc tatttattca tccggcgatt
    39661 gacgctactt tttcgttttt atattggtac agcgatcctt acaaccatga ccgtttcatg
    39721 gacccgggaa cggattctgt aggcattgct ttttctaaag aaaatatgtt agtcttattg
    39781 caagaagaaa acggagaaag tagtgatagc agtgatcgtt aacgaagagc tgtttcaact
    39841 ggaggatcaa tgcctccaag tcgctgaaat gataaaaaag agtaaaacga tggcttctta
    39901 tcttgaacat aaacagtcaa tggaagaatc tgcaaaagtt tgccagttga aagaagcgtt
    39961 cttgtctaag aaagaagcat ttgaaaatgt agcggcatat ggtgagtatg cacctgatta
    40021 tcgtgaaaaa aaacgagctg ttcgaaaagc aaaaagacag cttgatatgg atgagcatgt
    40081 tgcagctttc cgatatagtg aaacacagct gcaaagtctg ttggatcaaa ttgtccaagt
    40141 acttgccgaa gaagtttcta atgaaatcaa agtagatgca ggtagcccgt tttttgaaac
    40201 gaaaaaacac tttggatgcg gaggtaattg tcatgcaatt agacaatagt gaatttgaaa
    40261 taacaaaacg acgcgggctg atcgtatggg tctacagctt aaaacaatta aagaatctga
    40321 aaaagttcgg cttgttgcat tatgtctcaa gaagaatgaa atatgtcgtg ctttacttaa
    40381 atgaagagac attggaagaa acagaagaac gaatccgtaa attacatttt gttcgaaacg
    40441 tagaacgttc ttaccgaccg gatatcgaaa tgaattttgc cgaaaaaata ggaaacaaaa
    40501 cagtattaaa agacgaaggt ttcgaaattg aagagctaaa tacagaaatc cgattagctg
    40561 aacatcttta aaaatctctt atgagcagtt tttaaataaa aaataacttg gataacgatc
    40621 gatttttgat cattgtccaa gtattttttt ggtatactag taccagtacc atttctaagg
    40681 agtgagtaga tgcggataat atcaggaaat tatggcgggc gccgtttaaa aagtttagca
    40741 ggggcgaaca ccagaccaac tacagataaa gtaaaagaat ccatattcaa tatgattgga
    40801 ccatattttg atggagagac agtattggat ctgtttgcag gaagcggtgg tttagctatc
    40861 gaagcagtat ctagaggctg cagtcatgcg gtatgcgtag acaaaaatta ccaagcgcta
    40921 aaaatcatca aagaaaacat cgaaattacg aaagaaccag agaaatttac cacaataaaa
    40981 cttgatgcgg acaaagcaat cgaacggcta gcagcggaga aacaacaatt cgactacttg
    41041 tttcttgatc cgccatatgc aaaacaaaaa ataatcgatc aaatccataa aatggaagaa
    41101 ctgggacttt tttctaaaac cgcagttatc gtatgcgaaa cggataaaac agtcgaattg
    41161 ccagaaactg tggctggatt ccatcaaata agaaaacaga tttatggcat aacggccgta
    41221 actatctata gaaaagaggg aaatcaatga aacgtgcatt attcccagga agttttgatc
    41281 cttttacaaa aggtcactta gatacggtgg aacgtgcggc taaattattc gatgaggtag
    41341 taattggtgt ttttatcaat acttcaaaga aaagtttgtt tcccccagaa gaaagaatga
    41401 cattgatcac aaaagctgtt tctcatcttc ctaatgtaaa agtcatgcat caagaaaatc
    41461 aattgacggt agaaacagca aaagagattg gtgcagatgc gttgatcaga ggcatacgaa
    41521 gtatcaaaga ctttgaatat gaacgagaga ttgctcagat gaatcatcat ctatatgatg
    41581 aattggaaac tgtcttctta ttagcaaaac cagaatatag tcacgtgagt tccagtattt
    41641 taaaagaagt actgcacttt ggcggagatg tgagcagcta cttaccgcca gtgatcaacg
    41701 aagctttagc tggaaagcgt gaatcaaatg aaagctaaac aatggttgaa aacagtactg
    41761 attatcatat ttggaatgtt gatcattgga tttgtagtag ttcccttgcc gtattatgta
    41821 gaatcaccgg gagccacgat cgatctgaaa gaactaatta cggtcaatga taaggaagat
    41881 aaaggttctg ggtctttctc tcttacctct gttggtatca ggcaagctac aggtttctta
    41941 gctttaaaag ctaaaatgac cccttttgaa gaattgatca gtgaagaaga attgactggt
    42001 ggggcgacaa atgaagaata taatcaaatg caacagtatt atatggattc ttcccaaaat
    42061 gcagcaatcg aacaagcgtt aaaacttgca gacaagcctt ataaaatgga attcaaaggc
    42121 gtatatgtca tgagtgtaga gaaaacatcg aatttctttg gcaagctttc agttggagat
    42181 acagtaacaa aagtagacgg gaaaagcttt caaagtacag aagcatttat ggattatgta
    42241 aaaagccaaa aagttggaca aacaatcgaa atcagttatt tgcataatgg tgaagaaaaa
    42301 acagcttctg gtgatctgat cgagttacca acagataaaa aggcaggaat aggtatcacg
    42361 cttactgacc ataccgagat cgaatcagat acagatgttc gtattaattc tggctctatc
    42421 ggtgggccat cagcgggatt gatgttcact ttagaaatct atgaacaagt cactggtaaa
    42481 aacttgcgac atggtaagca aattgcagga actggaacga tcaacagtga aggagaagtt
    42541 ggccgaatcg gaggaatcga taaaaaagtg gcaagtgcgg ataaagctgg cgtagagatt
    42601 ttctttgcac cagatgatac gatttcagac gatgttaaga aagaatatcc tgaaataaaa
    42661 accaactacg aagaagcaaa agctgccgct aaaaaattga attcctcaat gaaaatcgta
    42721 ccagtaaaaa cagtacaaga agcgcttgac tatctagaga aaatgaaatg aaactaaata
    42781 attaaagagg ggttgtgaca cgagttctgt cacaacccct ctttttcgtc aaaaatcttc
    42841 acgataaaaa taaaaacaaa agaaaaaatt gacgtaatta gatgttgaag ggacgatttt
    42901 gccagagctc actgtttctt caattttaaa tgaaaggagt aatgaatgat ttttactggt
    42961 cattctttga aaaaagaaca atgatagaaa aaatcagcaa gaaaatacta gcttcaccca
    43021 aattgatcat ttggatgtct atgatagttc ttatcttttg tatcgcaagt ggttttttat
    43081 tatcaaattt gctattttat agagaaacag aagagactgc tgcaaaagaa atactttctt
    43141 cgtttagttc cagtggagaa gatccatcag ctgaaggaaa cgatgtagaa gaggatgctc
    43201 gtccaaaggt catgtacacc gatatcaaag gaagtgtgaa ggagcctggt atctattcct
    43261 tttcttcaga agaacgggtc tatgatgtac taaagcgtgc aggtggatta ttagaagaag
    43321 cggatagcga tcgaatcaac ttttctgcga agatagaaga tcagcaagtc ctatatatcc
    43381 ctgctgtagg tgaagagctg ccagaacatc ttgatcagtt ggcatcctcg gaaggaaaac
    43441 agcctacagc agattccgag cctcccaaaa taaatatcaa tacagccact ccatcagaac
    43501 ttcagcagat tcctgggatc ggatcagtca aagcgcaaga aatcattcga tttagagaag
    43561 aaaacggatc cttccagaaa gtggaagacc tgcaggagat ttctggtatc ggtgaaaaga
    43621 cagttgaaaa actaaaaaac ttcgttacaa ttaaatagaa tgattcaaaa tcgactggca
    43681 cgagcaataa atggacgcta atggatacta agaataatcc aattctaaga gcttggcagg
    43741 acgaggaggt agtatttttg gataggaaat agcttgattg ttcgcaaaga aacattaaaa
    43801 aaaccaatgc tgaatgtcta tctccaaaat aaaatatctg ggattcatat tatgaatacg
    43861 gctgtcagcg gcaataacag tcaagctcta cgagaacgct ttgccaaaga tgtacttagc
    43921 tatacagcag ataaagtatt tattctcatt gggacgaatg atctagcaga gcataaacaa
    43981 ctttctaaag aaacgtacca aaaaatctgc agtggatgat agttcagctg aaaaaagact
    44041 atagttctga tcatgctttc tttatcacgc caccagcagt cgatgagaca aaacaaaaat
    44101 atcggaataa caagttgatc aaagaatata tctctgttct cacagaagta gcaaaacgcc
    44161 atgattgtga agtatcgatt tttatacgga atttcttgaa caaatggatt cgaagaattt
    44221 ttataatggg ctaatggatg acggacttca tttcggcagt gaagggtagg atcttttgtc
    44281 agacaggatg attcgttata tttaaagaat attttgttta agcaatgagt actttttaat
    44341 caacttgaaa aaacggagga atataaaaca tgttagaaaa atcttcaaaa gcatgcgata
    44401 cgaataaaga aaaagttact gataagtcac ataatgaacg cattccttgg gaccagtact
    44461 ttatggctca ggcagtactg ttatctttac gtagtacgtg tacacgttta gaagtaggag
    44521 cgacactcgt tcgagaaaaa aggatcattg ctggcggcta taatggagca gtcagtggag
    44581 atgtccactg catagatgaa gggtgttacg tcgtggatgg acactgcttg cgaacgatcc
    44641 atgcagagat gaatgcactc ctacagtgtg caaaacttgg tatcccgacg gaaggttcag
    44701 aaatctatgt cacacatttt ccatgtttgg cctgtacaaa ggctttgttg caagctggtg
    44761 tgaaagggat caattatctc catgactatc gaaatgatcc ttatgcccaa gcgttgatcg
    44821 aacaattagg tgtatctgta caccaagttg ctttagactc acagcatttt tcaaaacttc
    44881 aaaatgaatt aacaggaaaa catgtctagc gttttagaaa ggcatgttcg aaatcggttg
    44941 atttttccgg caattacgtc gatgattatc tgtttgttgt attacactga caattggggt
    45001 tatcgtttgc ttcttgccgg aattttttta tggttttcgc taaaagaagg acgttttatc
    45061 gcgtacttgt gtattttgtg tagcataacg gtatccattt cttataggct acaagagtat
    45121 tcatttttag aggaaaaaac aacatctgcg caaattactg tatttacaga tacgattcgt
    45181 gcaaatggta attttttgac aatggaagga aggttatcag agaataaaca aaagctccag
    45241 ctttcttatc aagtaaacac agaagcagaa ttgaaaacat ggcttaactt ccgaggaaag
    45301 ggaatagaat tgactgctga aggcgagttc atctctccag caaaaaagcg gaatctttat
    45361 aattttgacc aagaagctta cttcaggaac catagaattt ctggacggtt ccaaattcaa
    45421 aagattgaac agatcaccat gcaaaatcac tggcgaaact ggcttcagag aaaaagagga
    45481 tcttttatct cctttgtcca atctgaattt ccgcaaaaaa caagtgtata tattaattct
    45541 ttattatggg gctataaaga tgcaggattc agagaaccag aagaaatttt tagggagagt
    45601 ggtttgctgc atctgttcag tctatcgggt ctacacatcc aattttattt agggtggctg
    45661 tattatttat ttcgacgtat cggatggcca ctacacgtca gtattattcc gttgagtcta
    45721 ctaactatct gttatgtttc tattgcagga agttctattt ctgttttgcg tgctagtttg
    45781 ctgtttttat tgcgattgct agtcaaattg ctgaataagc attattccac catggactta
    45841 tttgcagttg tcttttggct tcttttacta tttgatcctc gatttctttt tcatgcgggt
    45901 ggacagctta gttttttcat gagttttttg tttatcctga tttcttttga tggagattgg
    45961 ttgaaaaaaa cggcctctca cgtcctgttt actatgatca cgatgccgtt gattgtatgg
    46021 catttttatg agtggcctat tttaggtagt ctttttacat tactgattgc accggtattc
    46081 aagtttcttt ttcttcctgg tgtgttattt ctcacactct tcaatcgctg gctgccggaa
    46141 actttacttt tgctgataga agagggatta atcttatttg agcgaataat cgatttttct
    46201 tcagggtcgt ctctcattat aggacgtttg ccccttgtgc tatctgggat gtatattttc
    46261 ggattactga tttgtatgga taggttgaaa gaacaacctg tgaaacactt gttttatgtt
    46321 ggtctttttt cttttatttt tttgactttg ccatttttac gcttttcttc aacgatcgct
    46381 tttatcgatg tcggtcaagg agacagtatc tttttgcaaa gtccttttcg aaaagaaacg
    46441 attttgatcg atactggagg tcagttacac tttgaaagag aaaagtgggc gcaaggtatg
    46501 atacgtcctc ctgctgaaag aaatatcgtt ccatatctaa aaggtcaggg gataagtgta
    46561 atcgataaac tcatcttgac acacgacgat acggatcatg tgggagaatt acccacgtta
    46621 tctcagcatt ttactatcaa aaaaatttat atcggttggg gcgctggacg aaatgagcca
    46681 ttacaaaggc atttatctga agctcaaaaa aatggtactg agatcattga aatcaaacat
    46741 ggggacagga tcagcggtta ttatgatctt tatgtattga ctccttttga aaagggagag
    46801 ggaagaaatg aagattcgat tggtctatgg atggaatata acaataggcg ctttttattt
    46861 ttaggagatt tgaatcaagc aatggaaaaa caattgcttt tgatttatcc taatctgaaa
    46921 gccgatgtag tcaaattagg tcatcacggt agccgtacgt caagtaacac agattttctt
    46981 tcccatattg aagcaaaaca tgggattata tcctgcggcg taaataatcg ttatggtcac
    47041 ccgcacagcg aagtattgga gacactagaa gaaaatcaga taaaaacatg gcgaacggat
    47101 caacaaggaa tgatcaccta tcgctggcat ccagtttttc atcctcatgg gctagtggaa
    47161 acaatgatag actagtaatt ctcatctgct catggtagga taatttttga aagtggtgag
    47221 taatttgagt atacaagcag agttacaaaa aatacgtaaa gaaaaacttg catcttgtta
    47281 cttgatttta ggaacagaaa agtttctcca agatcaagtt cggacagaaa ttgtcaaaaa
    47341 aatccaatta gagggcgaag atgacttaaa ttttttaagt tttgacatgg aaaatgcaag
    47401 tttagatgag gtagtagctg aagcagagac gttgccgttt ttcggagatc aacggttagt
    47461 attcgtagag aatccttatt ttttaacagg tgaaaaagta aacaatggaa tcgaacaaaa
    47521 tactgattta ttgacggctt atttgaaaga accattagaa tcgactgtat tagtattctt
    47581 tgcgtcatat gaaaagttgg atgaacggaa aaaagtaacg aaacaattga aaaaaacagc
    47641 aattatcatc aatgtccaac agttgaatga aaaagaagta cgtcaatatc tgatgaatac
    47701 cttagagaat tcctcaatca aaatggaacg aagtgcaatt gatttattct tgaggttgac
    47761 cgacttagat ttatccaaaa tgatgagaga gttacagaaa ttggtccttt atggacaaaa
    47821 tcaacagcaa atcacggtaa gagaagtaga agaattagtt cctaaaacgt tagaacataa
    47881 tatttttgac atgacccaat atattttaaa agggaagacg gaacaagctt tgcgtttata
    47941 cgaagattta gttatgcaag gggaagaaac aatcaaaatt aatgcgattt tgctttctca
    48001 attacgttta tttttacaga caaaatttct tgtaaagatt ggttatcagc aggcaaatat
    48061 tgctgaaaca ttgaaaatcc atccatatcg tgtaaaactt gccatgcagg aagtaaaaaa
    48121 attcgatgaa catttgctgg tacagttgtt tgatcaatta gttgagatgg attatcaaat
    48181 caaaacggga caaatcgaaa aagaattaag ttttcaacta ttcgttttgc gcacagggca
    48241 gttgttaaga aatcgataag aagaaagacg agggcaaagc atgcgggaag gtcgtttgtt
    48301 tttgtccttt ttttgacaaa taaaaaaatc ggccgaagcc gattctttta tttagcgatt
    48361 tttttgctta aacgagattt gtcgcggtta gctttgtttt tgtggattag accttttgat
    48421 tcagccatat caatagcttt aacagcttct ttatataacg catctacgtt gtcagcacct
    48481 gaagctacag catcttcaaa ttttttgatt gcagtacgca tagcgcttgt ttgagatgaa
    48541 tttttcacat tagcgttttc gcttgtacga acacgtttga ttgcagattc aatgtttggc
    48601 attagtttca cctccgataa cttaagttct tacgcgtgtt accgcataat tcaacattca
    48661 ttattatacc taaaggcagc gtgctttgca atatgaacat gacaagtttt tttccttaac
    48721 agtcttttat taggattttt tttcagtaaa aacggatagt acaagctgaa aaaaatcaaa
    48781 aagacgctat tgtgggaggc aatagcgtca aaccaaacta gatttttatt tatctattta
    48841 aacaaagttt gaatgggaat gtttggtact aagttattat ataacaaaga gaaccgtttg
    48901 caaagttttt tttgaaaagg gatacgattc cctgttgttc tttgtttttc tattaagaaa
    48961 ctgcagttat tttataacca gtatcctttg atcagtaaag gctattctgg tgtaccgatt
    49021 attttaatgt attcatcgat attttttatc aacatttttt ttatgaccaa ctggttccga
    49081 cggttccatt tgccacttac ttccagcaca tatttattta ggggcaaacg taagatatat
    49141 tttgccaaat agcgatcttt aagcaggcca ttgatcacac ggtcctcaag ctgcaagttg
    49201 atcccagtca gcatcattgg ctgttcttgt aaaacgtacg tatcaattaa aagaccttca
    49261 aaagtttcca caagcgtctt cctttctttt caaagtaacc tgaaaacagg cactaattct
    49321 tccattttga tattcgttga agtggatgcg atcattcttt cttttgtcca ctgtaatcta
    49381 gtataacaaa aaagagcaga agagccaaaa agaaaaaaga gcatcgaatt ctataggaag
    49441 tctcattggc acggcctaaa ttattacaaa acattgatta aaaaaagccc ctgcatcgca
    49501 ggagcatccc ctatatcgtt ttaataggtc aatcaaaaag cagtatcaaa cacattttgt
    49561 atcaaatcag gaaataatag ataagctaag cccagaatca aaagtaccgc tagaagaaac
    49621 cagccgattg ttcctatgaa atactttaat ttttcgaaca ttgtatcacc tcgttgtcag
    49681 tatatcacgg atcagtaaat tgggggtgtt aagaatctcc taaaatagac aaaatttgtt
    49741 ctattttcct aaagcaaata cttgtaagat acagtaagtg ataccagaag caatgaccgg
    49801 tccggcggcg attcctctta agaagacaac acctaaaatc gtgccgaaaa caagtgcaac
    49861 agtgatttgt ggatcagaag caatcaatcc tacacccttg gaagataaga cagccactag
    49921 cccaccgcaa aaaacagcta cccagccaat aggggattta aaggcttcta atagatcacg
    49981 tagaccgatt tctcctgttg cgatagggat tagaatagcc gctgtgatga tcgtcacccc
    50041 aatattgatt cctttgcctt gaagccaagg gaaaaaggaa cgggaggctg gtatgagctt
    50101 cataatcaaa acaaaagctg ccgctatcat cagactttga tttttagcta aaattgctac
    50161 aagtaaaata attcctaaaa aaagccaact ttccatgaaa tatattcctc actttcttcg
    50221 aaacaatcat aacatgtttt tagaaagtta caaaagtagg aattcatcct tcaagaccta
    50281 ttcataagct gtttttttca taaaataaac aaaatcataa aaaaacactt tgcttatttg
    50341 acagaagaca gccgcgcttt gtatagtgga caagacttac atttttttga agaatcagaa
    50401 agatcaggta aaggtcttag taaacgcaag ccattcagaa taaccaatat tgtgcttcct
    50461 tcgtgtccta caactccaag aggtagattt aaaaattgca ggaagttaga agcaatcaac
    50521 aagagaataa caagaatcga aaaaataata ttttgtttga tgatccgccg catttttaaa
    50581 gatagccgat gactgtagac aaatttattt aagtcgcttt tcattaagac gatgtctgcg
    50641 atatccatag cggcatctgt cccttgcccc atagcgacac ctaaagaagc ggctgctaaa
    50701 gcaggcgcat cattgactcc atcaccaacc atcgcattta cttggtaaag ttcattctct
    50761 tttttgacta gttgtgtttt ttcttccgga gtacagcttg catagtaatg ttcaatccct
    50821 acttgttcag caatggcctt tgctgtgcca gaatggtcgc cggttatcat acttgtatgg
    50881 atgttttggg atttaaagta ggaaattact tgttgggtat ttgcttttgg aatatctagc
    50941 agacctaaaa cagcaattaa ctgctgatct ttagaaagat aaatcacggt tttgccttga
    51001 ttttccaaac gttcgatcat ttccgcaatc tctttgctga ttatcatcgt ctcaggatca
    51061 tatgcgtgct tgccaacttt ccattgcgca cctaaatagg tagtttgtaa tccaaaacca
    51121 gtaatttctt ctattggtaa gtattggatt tcttcaggta ttgtgcaatc gaaatattgc
    51181 aaaatcgcat gagcaagtgg atgcgtcgtt ttttgttcca tagcaacaag aagttgttgt
    51241 gccaaatcct tttcatcaaa gaaaaagctg tcagttacaa ctggttttcc tttagtgagc
    51301 gttcctgttt tatcaaaggc aatcgctttt aattcagaaa gcttttctaa atggataccg
    51361 cctttgatta aaatcccatt ttttgcagaa tttgaaagag cagctagaga agctggtgta
    51421 gcagatgcga caagcgcaca aggagaagcc acaactaata ataccattcc tcgataaaag
    51481 ctttcatccc aactccaacc taggaaaaag tgggggagaa ggatcattag aggaacagca
    51541 agcaaaatca attttacata attattttcg attcgttcga taaatgaagc tgtttgagtt
    51601 ggcgtattct gcgcttcttc tactaattgg ataatcttag caaagacagt ttcggaactc
    51661 gtttttgtta ctcgaatcgt gatgggattt ccttgattca gcgttcctgc atacatctca
    51721 tcgcctaaat atttttctac aggcacagat tcaccgctta atgcagactc atcgatcgtt
    51781 gcctgtgtgc tttctaaaaa gccatctatc ggaacagctg ctcctttagc gacaaatacc
    51841 aagttatcca cctttagttg agaaacatct acttcttgca tttgtccatt ttcttttaat
    51901 aattgggctt tttgtggttg aagattcatc aaagcagtaa tttctttttg acttttatta
    51961 gttgtgtatt cctctaatgc accgcttaaa caaaagatga atgtcaacat tgctccttca
    52021 aagtaattac cgatcaaaca ggcaccaata gcagctaaag ccatcaataa atccacattc
    52081 agtgtccgat cattccacaa ttctttcaag ccttccgatg tttgtttata tccaccgatg
    52141 ataatggcac tgatataaaa aagtggggtg atggtaagag agaatcggtc gaagataaaa
    52201 ccgaagatca taaataaaag gcagccgatt gttgctgcaa aagctttttt ctgattagtc
    52261 atcgttatta gacctccttg tcaaaacaag gatagcataa taaaaatgta attgataata
    52321 ataacagcta aatgagaacg ataatggtta tcatttggtg attaagcttg atgttgtttt
    52381 tattatttat aatgattatt attaaagaaa ataaaaaacc agaacatttc tcaaataaga
    52441 gaagtgttct ggttttttct acattgcaat aaaccaataa ccaatcaaaa tgataccaag
    52501 gataatacga taccagccaa aagcggtaaa gtcgtttttc ttcaaatagc ttagtaagaa
    52561 tttgatagct aagacggaaa ccacaaaaga tacgaatgtt cctacaagaa gaacaacagt
    52621 ttcttccatt ttgaatgaat tcccttcagc taaatatttg acgatcttca agccgcttgc
    52681 gccaaacatg acaggaatcc ctaagaaaaa tgaaaactct gtagcgacaa aacgcgaagc
    52741 accaatcaag atagatccta ggatcgttgc gccagaacgg gatgttccgg gaacaagtgc
    52801 caagacttgg aataacccaa caagaagagc tgcttggtac gtaaagtcat tcagagaagt
    52861 ccaacgaggt gttttgtttt tgttgcgttt ttcgacaata ataaaggcaa taccgtaaac
    52921 gatcaaaaca atggcaactg gtaagaactt gtggaaattt gcttctaacc aatcatccaa
    52981 aggcaaacca atgatgatgg ctggtatgca agcaacgatc actttaaacc aaagtgtcca
    53041 cgtatctttt ttttctaccg ttgatttact tggagaaaat gggtttaact tgtgaaaata
    53101 aagaacgacg acagccaaga tcgcccctaa ttggataacg acattgaaca tcgacataaa
    53161 ttgtgaactg gcatccagtt tgatgaattc atctgctagg atcaaatgac cagtactact
    53221 gatgggcagc cattccgtta ttccttcaat aataccgaga atcactgctt tgataatatt
    53281 tgcaaaaaac ataaattcct tccttcccaa ttaagataca tcttatagta taccgttatt
    53341 ttttgagaaa cccaatctgt tttttaatat taagaaaaac ttgagaaata aagcagataa
    53401 gtacacgaag agaaaaaaga agacaaagcc ctcgttcaga ctttgccttt tatgattctc
    53461 tggtaaaaat taaaatatcc ccattataga aacgagtcgc gactatgtgt tttcccgtta
    53521 tcgatataag acgtatcatt gatgccgtgg atagaaaatc gaccgtttgc ataagtgacc
    53581 tttgttacgc tgccatttaa aagcccagca cgaacaggct gtttaggatc gatcagatta
    53641 agcaaaaagg cgatcgtcaa accatgagat acgactaaaa catttccacc accatttttt
    53701 tcgcagtgat gggcgatatc ttcaaatcct gtccatacac ggtctcgcaa tgtttcataa
    53761 gtttccgccc acccagcggt atctaagttg tatacagcat tagcaatttg ttcaaaagaa
    53821 acttctgtgg taaacatttc atcatatgtt ttaaaattca atacacgagg gatcacaccc
    53881 cacatttcca tgtcatatcc accttcaaga gacccgaaac accattctct aatccttttt
    53941 tcaataaaat aaggaatttc ttttccctta gaatgttctc ctagaataat acgggcagtt
    54001 tcgattgcac gaccactgtc actagaataa gcaaatgaaa agtcgacatt ttttaacccg
    54061 cgacctaaat aatgaatgcc ttcttgtcct tgctttgtga ggggtgtatc acaccagcct
    54121 tgtactcgtt caattgtatt gaacatcgtt tttccgtgtc gtacgatata aagtgtagtt
    54181 tctgtcattt tattctcctc ctaagtcgtt ttaacgattg aaaaaaggat ttgtcctttt
    54241 ttcctgttca atcgtcgttg catcgccatg tcctggatag actgcaaatt catcaggtaa
    54301 tacaaacagc tgggtccgaa tactatgaag taattgttct aaattccctg tatacagatc
    54361 ggttctaccg atacttcctc taaacagggc gtcgccagaa acgacaaaat catcaaaaat
    54421 aaagctgacg cttcctatag aatgccctgg ggtagggacg acgcgaaaag acatgtttcc
    54481 taaagtataa gttttcattt caaattcata ttctgcaggc gagacaataa catcagcaat
    54541 atcatcatgt ctaccgagtc cagaaagatt caaaatcgga tcacctagcc atttttgctc
    54601 tagtgggctg acataaactg ggatttgata atacgcgcgt aaaggttcca ctgcaccgat
    54661 atgatcgtag tgagtatggg taagcaaaat cgctaccggc tgttggtttg tttttttgat
    54721 ttcttgtttg attcgttccg catcttctcc agggtcaatg atcaaaagtg cttcttcgtt
    54781 gtagaccaaa taacagtttt cttcaatcat tcctgttttt aatctttcga tatgcatgtg
    54841 cattcctcct tgatagccaa aacttctttc atccattagt ataacgcatt cattgctagg
    54901 aaacaaaaac aggtacttat ttctctttac ttatctgaat tatcagacta tatgaaaagt
    54961 atttggatat tctaaagaga aggaaagtaa gttttcatag cattttaata tgtccttgtt
    55021 acattagatt catccaaaaa tccaaattct atatcctata tccctgccta atcagcaggg
    55081 tgttttttta tgtttggaaa gagagctctt tttgctaaac taggtttttt caggtaatct
    55141 tagagagaaa gaggtggtgg aaatgatcaa acagtcaaaa atatctgtgc ggcacttagt
    55201 agaatttatt ttgcgacgtg gaagtatcga caatcgtaaa aaaagtaacc atactgcatt
    55261 agaaggagcg agaatccacc gcaagctcca aaaagatgcg ggagaaaact atgaaaaaga
    55321 agtcttttta aaaacaacag ttcaattaga tgaatattac ctgatcgttg aaggccgtgc
    55381 agacggtatt ttcaaaagag acggtattta ttatatagat gaaatcaaaa cctccgaacc
    55441 acgatttgaa gatttagaac cagaacaagt ggaacttttc tttcatcaag caagggtgta
    55501 tgcctatatc tatagtcaag aacatgattt acaagaaata aatctgcaat tgacttactt
    55561 ccagacggca gaagaagtga tcacgagaaa ggttgaacat cagacaagag agcagttaga
    55621 aacttttttt aaaaagctga ctgaagatta taaagaatgg cttgtttttc aagaaaactg
    55681 gcgcactgta cgcaatgcct ctttgatggc tttgaaattc ccccatgatg agtataggaa
    55741 aggtcagcgt gaactagcgg tagctgctta taaaacgatc cgtacgaaac agaaattgtt
    55801 tgtagaagca cctaccggaa caggaaaaac aatctctacg ttattccccg ctttgaaagc
    55861 tgtaggggaa gaagagggag aaaagatctt ttatctaacg gctaaaacaa taactagaca
    55921 agtagctgaa gatgctatga cagcattaaa agatacagga gctgaagtaa aaagtgtcac
    55981 tttgacggcg aaagacaaga tctgcttttt agatgagacg acctgtaatc cagatcagtg
    56041 cccatatgcc aatggttatt acaatcggat taatgaaggt ttatgggatc ttttgaatca
    56101 tgagaatcag atcactcgtg aagtcatcga aacttatgca agaaagcata cactttgtcc
    56161 tttcgaacta tcattagatg tcagtatctg gtgtgacgta atcattgggg attacaatta
    56221 tttatttgat ccaactgttt atttaagacg tttttttgaa gatgaaaaaa acgaggacta
    56281 tctgtttttg attgacgaag cacataattt ggtgaatcgt tcccgagaga tgtattcggc
    56341 agaactatct tatgaaaaaa cgaaacgaag taaggaagct ataccaaagg aattcaaaaa
    56401 acttcatcgc cgtttcaaca aactattaaa agaatttgat tcgatccgag aaatagcaaa
    56461 agaagatcat tgggattatc atcatcaaaa agcaccggct gaatctttag taaaggcagg
    56521 ttatcaatta agcgaaaaaa ttaaagaatg gctggcagaa tttcctgagc acccatcaca
    56581 agaacaattg cttccttatt attttgattt gcttcatttt ttgaaggtca gtgaatttta
    56641 tgatgatcac tacgaaacaa cagtcgaaaa aacgtatcgc gatctgatag taaaagagtt
    56701 ttgtattgat ccaagcttgt ttcttgagca gtcgttggat aaaggaaaaa gcagtattct
    56761 tttttctgct agtttttcac cactctctta ttatcaggaa acactggggg gacaaaaaag
    56821 tctggcttac aaactgccga gtcctttccc agaaggaaac cagcaagttt tcatcgcaaa
    56881 ttacctagag acgacttatc gaaaaaggga acacagtctg gcaaaattag tagagacgat
    56941 tcatgctttt gctgatggaa aggtcgggaa ttatttcgtt ttctttcctt cttatcaata
    57001 tttagatctt gcggtggaag cattcagaca gcaatatccc gacagcaatg tgctaatcca
    57061 agaaacggac atgaatgagg aagaacgcga acgattttta acaaaatttc aaacaaatcc
    57121 cgctgaaaca ttgattggat tttgcgtact tggcgggatt ttttcagaag gaatcgattt
    57181 gagaggggac cgattgatcg gcagtatgat cgtaggtgta ggtttgccgc agatgaatca
    57241 tgagcaggaa ctgatcaaat catattatga tgagaaggaa caccttggat ttgcttacgc
    57301 ttatcagctt cctggaatga acaaagtctt gcaagctggt ggtcgagtga ttcgtggcat
    57361 ggcggatcaa ggaatcgttg tattggcaga tcaacgattt tctagttttt cttatcgacg
    57421 tttattccca caacattggc aaacagcaag agaagtacga agtgttgggg aattggaaga
    57481 aggaatacag cgtttttggt tatcaaaaga agcagtaaat caaaaaaacg aggaatgaca
    57541 gacgtcagcc tcgttttttg attaacgata gttagtgaat tgcaactcga ttggtaagtc
    57601 aagtttacgt aataaagcaa tcacagcttg tagatcatca cggttcttac ctgtcactct
    57661 aatctgatct tcttggattt gtgcttttac tttcagtttt tcatttttga tagctgtagt
    57721 gattttcttc gcgttttcac gatcgattcc gttgaccaaa tcggcagttt gtcgagcttt
    57781 gccacctaga gcgtgttctg attcggaaaa atggatattt ttgattggca catttctttt
    57841 gatcagctta ccaaataata catctttgat ctgttcgatt ttgaaatcat catcgctcaa
    57901 tacaactaga ttgctgttct ctaatttgat ttctactgta gaatccttga agtcaaagcg
    57961 attggacaat tcttttgttg tttgctgaat cgcatttttt acttcttctg tattgacttc
    58021 tgatacaatg tcgaagctag catcttttgc cataggaacc ctcctgatat acatataaat
    58081 atttaccata catctatctt atcataggat tagggcagat tcttacctgt ttgctaaaat
    58141 tcttctcatt tgagttcgaa gtattttatt ttaatagata aactgttatt cttacatttg
    58201 gaataccaaa gtaggggaga ggtgcttgtg agtaaaatta tgactgcgta tcataaaaat
    58261 gaaagattaa agaaaatagt ggttattttg ataaccggat tgaccgctgc ggtcgcttta
    58321 aattattttt tgattccagc caatgtcttt tctgcaggga tgaatgggat cgctcaaatc
    58381 atttcctcgc ttttatatga atggtttcat atcaagatag acacaggaac atttattttt
    58441 cttttgaata tccctgtgtt tgtattaggg tttctcaaag taggaaaacg agcaacgatc
    58501 cttagttttg tgaatgttgt atgtgtatcg gtgatgacga tgctgcttcc agtcggacag
    58561 atcactgata atattttgat gaatgcccta gtaggcggtg ttttattagg tcttggcgta
    58621 ggattatctt taaaaatggg ctttacaact ggtgggatgg atatcatctc tcttgtactt
    58681 tcacaaacaa ctggaaaaac agtcggaaac tacatgctga ttctaaatgg tatcattgta
    58741 gtcatcgcag gcttcttctt taactgggaa agtgccttgt atacgattat ttccatttat
    58801 gcaatgactc aagtaattga tgccatccac acgagtcatc aaaaagtaac agcaatgatc
    58861 gttaccgtca agccagaaga ggtaacagaa gaactagcaa aacggatggt acgtggaatg
    58921 acattactgc cgtctgtcgg tggctattca aaaaaacaag ggaacatgat catgatggtc
    58981 atcacccgct acgaaatgta tgatcttgat cagatcgttc atgaaatcga cgacgatgca
    59041 tttatcaata ttttaccaac tcattcagta tttggccggt ttgcaaatga aaacgaacaa
    59101 aaaatgtatc gaacaactgg cgtattgcca gaaataaaaa atcgttcaca aaaaaaatga
    59161 ggaatcaggt ttattttaga ataaatttaa tctattacat ctaataagaa catttttata
    59221 aatttaaacg agaatcttac ttctaaattc tcatttacaa gcagctaaaa aaagagtatg
    59281 ataaataaaa atcgtgttaa ggagtgtcgt acgatgagcg aagcaaacca agaacggtct
    59341 ggacaagaaa tagaactaat caaagaacgt attcttgctg ccttggaaat ggtgattgat
    59401 cctgaattag gaatcgacat cgtcaattta ggcttgattt atgatataga attcaatcct
    59461 gaaaatggag aaacagtcat taagatgact ctaacaacta tgggctgtcc attagcagat
    59521 attttaacag aatctatcca tgacgcatta aaagaagtgc cagaagtaac gaaagcagaa
    59581 gtcaaactag tctggtatcc agcatggacg acagataaga tgagccgtta tgcacggatt
    59641 gcgttgggga tacgatagaa atcttggctg actagctatg agaagcaaat aggaaaaaca
    59701 aaaaagcctt ttatcctttc tagaagaaaa gagataaaag gtttctttgt ttataaaatt
    59761 ttgtctgcta tattataagt taattaaata aatgtgttct attgataaag gacattcaaa
    59821 tccctttcct tttggaatcc ttctttttta catcagtttg attttcgtat agtattcttt
    59881 ctcatcttca cgataaatcg cagccccatt tttttgttgt gctttaagac tgccaagatt
    59941 ttttttatt
//
LOCUS       DANKEF010000013        56379 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000013 DANKEF010000000
VERSION     DANKEF010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56379
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.013"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            320..1165
                     /locus_tag="PWZ48_000891"
     CDS             320..1165
                     /locus_tag="PWZ48_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2260565.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1446..2666
                     /locus_tag="PWZ48_000892"
     CDS             1446..2666
                     /locus_tag="PWZ48_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HDL2260566.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2663..5383
                     /locus_tag="PWZ48_000893"
     CDS             2663..5383
                     /locus_tag="PWZ48_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2260567.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5367..6311
                     /locus_tag="PWZ48_000894"
     CDS             5367..6311
                     /locus_tag="PWZ48_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2260568.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6380..7390)
                     /locus_tag="PWZ48_000895"
     CDS             complement(6380..7390)
                     /locus_tag="PWZ48_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2260569.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7570..7914)
                     /locus_tag="PWZ48_000896"
     CDS             complement(7570..7914)
                     /locus_tag="PWZ48_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2260570.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7907..8341)
                     /locus_tag="PWZ48_000897"
     CDS             complement(7907..8341)
                     /locus_tag="PWZ48_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HDL2260571.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8479..9216
                     /locus_tag="PWZ48_000898"
     CDS             8479..9216
                     /locus_tag="PWZ48_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260572.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9209..10420
                     /locus_tag="PWZ48_000899"
     CDS             9209..10420
                     /locus_tag="PWZ48_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260573.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10624..11406
                     /locus_tag="PWZ48_000900"
     CDS             10624..11406
                     /locus_tag="PWZ48_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HDL2260574.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11476..12138
                     /gene="trmB"
                     /locus_tag="PWZ48_000901"
     CDS             11476..12138
                     /gene="trmB"
                     /locus_tag="PWZ48_000901"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDL2260575.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12236..13990)
                     /gene="ade"
                     /locus_tag="PWZ48_000902"
     CDS             complement(12236..13990)
                     /gene="ade"
                     /locus_tag="PWZ48_000902"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDL2260576.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14122..15051
                     /gene="tadA"
                     /locus_tag="PWZ48_000903"
     CDS             14122..15051
                     /gene="tadA"
                     /locus_tag="PWZ48_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HDL2260577.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15008..16057
                     /locus_tag="PWZ48_000904"
     CDS             15008..16057
                     /locus_tag="PWZ48_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HDL2260578.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16054..16332
                     /locus_tag="PWZ48_000905"
     CDS             16054..16332
                     /locus_tag="PWZ48_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDL2260579.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16307..16777
                     /locus_tag="PWZ48_000906"
     CDS             16307..16777
                     /locus_tag="PWZ48_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HDL2260580.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16794..17096
                     /locus_tag="PWZ48_000907"
     CDS             16794..17096
                     /locus_tag="PWZ48_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260581.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17113..17484
                     /locus_tag="PWZ48_000908"
     CDS             17113..17484
                     /locus_tag="PWZ48_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HDL2260582.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17456..17815
                     /locus_tag="PWZ48_000909"
     CDS             17456..17815
                     /locus_tag="PWZ48_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260583.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17987..18994
                     /locus_tag="PWZ48_000910"
     CDS             17987..18994
                     /locus_tag="PWZ48_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2260584.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19019..20203
                     /locus_tag="PWZ48_000911"
     CDS             19019..20203
                     /locus_tag="PWZ48_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDL2260585.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20640..22223)
                     /locus_tag="PWZ48_000912"
     CDS             complement(20640..22223)
                     /locus_tag="PWZ48_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2260586.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22801..22944)
                     /locus_tag="PWZ48_000913"
     CDS             complement(22801..22944)
                     /locus_tag="PWZ48_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HDL2260587.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23167..23646)
                     /locus_tag="PWZ48_000914"
     CDS             complement(23167..23646)
                     /locus_tag="PWZ48_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2260588.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23719..24003)
                     /locus_tag="PWZ48_000915"
     CDS             complement(23719..24003)
                     /locus_tag="PWZ48_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260589.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24081..24275)
                     /gene="ssrS"
                     /locus_tag="PWZ48_000916"
     ncRNA           complement(24081..24275)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PWZ48_000916"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24347..25633)
                     /locus_tag="PWZ48_000917"
     CDS             complement(24347..25633)
                     /locus_tag="PWZ48_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HDL2260590.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25771..26442
                     /locus_tag="PWZ48_000918"
     CDS             25771..26442
                     /locus_tag="PWZ48_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HDL2260591.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26408..26896
                     /locus_tag="PWZ48_000919"
     CDS             26408..26896
                     /locus_tag="PWZ48_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HDL2260592.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26909..27856
                     /gene="prmA"
                     /locus_tag="PWZ48_000920"
     CDS             26909..27856
                     /gene="prmA"
                     /locus_tag="PWZ48_000920"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDL2260593.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27857..28621
                     /locus_tag="PWZ48_000921"
     CDS             27857..28621
                     /locus_tag="PWZ48_000921"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDL2260594.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28854..31067
                     /locus_tag="PWZ48_000922"
     CDS             28854..31067
                     /locus_tag="PWZ48_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HDL2260595.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31078..31524
                     /locus_tag="PWZ48_000923"
     CDS             31078..31524
                     /locus_tag="PWZ48_000923"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDL2260596.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31695..32954
                     /locus_tag="PWZ48_000924"
     CDS             31695..32954
                     /locus_tag="PWZ48_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HDL2260597.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33043..33990)
                     /locus_tag="PWZ48_000925"
     CDS             complement(33043..33990)
                     /locus_tag="PWZ48_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2260598.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFHIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34384..35676
                     /locus_tag="PWZ48_000926"
     CDS             34384..35676
                     /locus_tag="PWZ48_000926"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDL2260599.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35691..37460
                     /gene="aspS"
                     /locus_tag="PWZ48_000927"
     CDS             35691..37460
                     /gene="aspS"
                     /locus_tag="PWZ48_000927"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDL2260600.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37569..38444
                     /locus_tag="PWZ48_000928"
     CDS             37569..38444
                     /locus_tag="PWZ48_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2260601.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38710..40593
                     /locus_tag="PWZ48_000929"
     CDS             38710..40593
                     /locus_tag="PWZ48_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2260602.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40668..40952)
                     /locus_tag="PWZ48_000930"
     CDS             complement(40668..40952)
                     /locus_tag="PWZ48_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HDL2260603.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40942..41676)
                     /locus_tag="PWZ48_000931"
     CDS             complement(40942..41676)
                     /locus_tag="PWZ48_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HDL2260604.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41805..42428
                     /locus_tag="PWZ48_000932"
     CDS             41805..42428
                     /locus_tag="PWZ48_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDL2260605.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42620..43741
                     /locus_tag="PWZ48_000933"
     CDS             42620..43741
                     /locus_tag="PWZ48_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HDL2260606.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43748..46876
                     /locus_tag="PWZ48_000934"
     CDS             43748..46876
                     /locus_tag="PWZ48_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HDL2260607.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46895..50089
                     /locus_tag="PWZ48_000935"
     CDS             46895..50089
                     /locus_tag="PWZ48_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HDL2260608.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50235..50942)
                     /locus_tag="PWZ48_000936"
     CDS             complement(50235..50942)
                     /locus_tag="PWZ48_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2260609.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            51707..52105
                     /gene="spxA"
                     /locus_tag="PWZ48_000937"
     CDS             51707..52105
                     /gene="spxA"
                     /locus_tag="PWZ48_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HDL2260610.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            52437..53090
                     /locus_tag="PWZ48_000938"
     CDS             52437..53090
                     /locus_tag="PWZ48_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HDL2260611.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            53316..54314
                     /locus_tag="PWZ48_000939"
     CDS             53316..54314
                     /locus_tag="PWZ48_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HDL2260612.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            54490..56298
                     /gene="pepF"
                     /locus_tag="PWZ48_000940"
     CDS             54490..56298
                     /gene="pepF"
                     /locus_tag="PWZ48_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HDL2260613.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        18547 a         9727 c        11685 g        16420 t
ORIGIN      
        1 gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt gaactaatag
       61 agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat agttggttat
      121 tataaaatag taatatagct gatttggatt caattcatat ttatttataa atcaaaaata
      181 cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta cttacttaaa
      241 ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc agtgaattta
      301 taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt tagacggtac
      361 tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga aacatttacc
      421 ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta ataatattct
      481 caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg gatttgatgg
      541 agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa ttgattctgt
      601 cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat ctcagaatta
      661 taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta tcaaagagct
      721 aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata atattgagga
      781 aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc ctaatcgtca
      841 attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg ttactgaatc
      901 ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg tgtttgatcg
      961 tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag atagtttaaa
     1021 tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata gtcatcaaga
     1081 aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag tatctcaagc
     1141 atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata gaattctaaa
     1201 tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt caaacgaagc
     1261 gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt tttttcgaat
     1321 aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac gaacaaattt
     1381 tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa aggaggaaat
     1441 ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca tttgaaggac
     1501 tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg accgttttat
     1561 caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt gcaggagata
     1621 attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa cagatgaaac
     1681 ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat ttctatcaaa
     1741 aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc gaaacggtag
     1801 agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga tttagttatc
     1861 gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa ctcacagatt
     1921 accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac tatgctcctt
     1981 tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg catatccatg
     2041 tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct caaggtcata
     2101 ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa ggaagttgcc
     2161 aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata tcattgagga
     2221 aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca aaagtagagg
     2281 atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat acagatgttt
     2341 tggaacggat acaatcagga gagctattag actatttgtt agaggaattc tcagatagga
     2401 taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat acgaagaccc
     2461 cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag acacctcaag
     2521 atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga atattgagcg
     2581 aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt aatcaagatt
     2641 ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg cttttggaaa
     2701 atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg ggggaaacga
     2761 agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt tcccttcaaa
     2821 aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg gcaaaatatg
     2881 gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc aaaatcgcgg
     2941 aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag aaaaactgat
     3001 cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag agcagttgat
     3061 ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt taggcatcac
     3121 gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac aacaattatt
     3181 caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga aagagctgca
     3241 agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga tccaacaaga
     3301 acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg cgaacaaaca
     3361 attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat ggcaatctct
     3421 caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc tggagtcttt
     3481 ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa cactggaaaa
     3541 gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc aggagcagga
     3601 actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag aatggcaaag
     3661 attacaaatt gagactagag aaacaaattc agaattgaag attttggaac aaaggtggca
     3721 ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga agcatcttct
     3781 ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc tccaaatagc
     3841 taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga cctatccagc
     3901 attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt atatgctgat
     3961 ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga aagtgatcct
     4021 tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac caaaaattaa
     4081 aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag gacaacttga
     4141 tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg gagaagaaaa
     4201 acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag gaaagatgga
     4261 tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga aattatatgg
     4321 cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac gacaaatcga
     4381 ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa tttcagaaaa
     4441 aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg ccagaagcta
     4501 tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac aaaaagtatg
     4561 gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt cggaagtacc
     4621 tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca atgaattatc
     4681 ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat ctgtaagact
     4741 gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag caagtgaaaa
     4801 tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat tggacgaact
     4861 ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg aatggggagc
     4921 acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg aaagacaatt
     4981 accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag gaaattatca
     5041 gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag aatttcccat
     5101 ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat ttgcttacct
     5161 ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct ggcttcatta
     5221 tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag aaaagtatca
     5281 aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga tgaaccaaat
     5341 cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta atcgatgagc
     5401 agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa aacggaaaaa
     5461 aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa tactgggatg
     5521 cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta aacggtaaaa
     5581 gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta acaagggtag
     5641 atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa gaagaaatgg
     5701 aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat cgtattgttc
     5761 gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca aaaagaaacc
     5821 atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac ttagggaaat
     5881 caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt atcattttgc
     5941 atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat acgcttgcag
     6001 gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc tgtcttgcgt
     6061 tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt ttagcacacc
     6121 atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca gaaatattac
     6181 atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt cgtcaaacag
     6241 aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc tatgtaccaa
     6301 aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag acataagtct
     6361 tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt gaagattctt
     6421 ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt tcacttgagt
     6481 ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag gcatcatctt
     6541 tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg aatgcattgt
     6601 cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta tccatgtcat
     6661 tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca taagaagtag
     6721 gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa gctgcttctt
     6781 ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg ccatcttctt
     6841 ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct ccatcatccg
     6901 ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt tgtgcttcta
     6961 cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata tctacgtgag
     7021 ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa gtatctttcg
     7081 tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc gcttgtttgt
     7141 cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta ttgaatactt
     7201 tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt gcttcatcat
     7261 agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta ttagtatcgc
     7321 ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa ataattgatt
     7381 tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata ttaactatac
     7441 catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt caaaatttca
     7501 tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct gttgagaaaa
     7561 taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt tttctacttg
     7621 ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga tatctttttt
     7681 gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt cgatcaatgc
     7741 gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg cttcttccaa
     7801 ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata acagacctcc
     7861 gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa tcatcgcagg
     7921 acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca taactatctt
     7981 gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg actaaatgga
     8041 tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag atattaagac
     8101 ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca cgcgaaaaaa
     8161 cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt ttaggcacaa
     8221 ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc ttgtcgtctt
     8281 catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata cagttttcca
     8341 tataaaacat cctttctata aagaataaat acttcagctt aactttacca taagttaaag
     8401 ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata aataaaaata
     8461 aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg gtacggacat
     8521 ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt agggttgatt
     8581 ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct gctggaagct
     8641 tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga agaatatcgc
     8701 aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac attgagagaa
     8761 catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat gaaaagagca
     8821 gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc agctaatttt
     8881 tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga gccttcttta
     8941 tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc attgcttgaa
     9001 ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca tattttagct
     9061 actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat acgagcagac
     9121 ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt ggacgaaatc
     9181 tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac gtttagcaag
     9241 acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt ttgttttagt
     9301 ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga aaaccctgca
     9361 gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct gtttattcgt
     9421 gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc ctaaagaaaa
     9481 agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc cgttgatctt
     9541 tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg agcaatcctt
     9601 ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc atctgcaaat
     9661 caagagagca gaattgtttc gactagatca acaagttatt cgttattgga aattaggctg
     9721 gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat gggcaggaat
     9781 agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc aacaagaaaa
     9841 aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc agatctatca
     9901 atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa gaagacgcta
     9961 tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc tttacttgtt
    10021 tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat tagtaattat
    10081 aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta tcggtttctt
    10141 atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc gctatatggt
    10201 tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc agtatctact
    10261 cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag tcgtattaga
    10321 tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc tctttgttaa
    10381 actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct gtgtagggta
    10441 aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct attttttttg
    10501 agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt gcttgacgtg
    10561 tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag gacgggagaa
    10621 tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa aggcgacaca
    10681 ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg aaacacgact
    10741 ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg gacaaaacga
    10801 acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt gctgactcct
    10861 aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca tcattcaaat
    10921 tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga gaagatgctg
    10981 caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct gcaaattgtc
    11041 catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc agtagtacac
    11101 ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct agtcgattgg
    11161 gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg aaattatgtg
    11221 ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac tgacgaaatg
    11281 cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac cagatattgc
    11341 aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg aattttcagt
    11401 ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat tagaggaaag
    11461 gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat gttagcggct
    11521 cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa tgaattgttt
    11581 gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt catcactgga
    11641 atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt cagtgttgtt
    11701 tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact tctacatgtg
    11761 gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat ctatctgaat
    11821 ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata taagactttt
    11881 cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa aacagataat
    11941 caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat cctcaaacag
    12001 gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga gtatgaagaa
    12061 aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca agataagacc
    12121 aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat atccataaat
    12181 aaatcccaga aaatgaacat tacagatgta atgtattttc tgggatttat ttgatttaaa
    12241 atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga gaaaatgaaa
    12301 ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag aaagaaagtg
    12361 tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt attaattcat
    12421 tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct ataggaagag
    12481 aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct ttttgaatca
    12541 aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact agattatgcg
    12601 agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct ttcactatgc
    12661 ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt aattggtctt
    12721 tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc aaatctttag
    12781 tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa agaagcagct
    12841 ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac gtatatgaat
    12901 ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact aatgttccct
    12961 tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag aaatcagctt
    13021 gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct gcattcaatg
    13081 ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga cggacgatat
    13141 gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg acaaagaagc
    13201 atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct tttgcaacgg
    13261 tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct tcttttgcag
    13321 ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac tcttccatgc
    13381 cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat tggatctttt
    13441 ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca gctaagccta
    13501 aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt gcaccattgg
    13561 tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc atgagtgttt
    13621 gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt cctatttcat
    13681 ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa aattcgcttg
    13741 gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc actagatatt
    13801 gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg atcattcctt
    13861 cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg atcttagcat
    13921 gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat ttccttaatg
    13981 ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata aattcggttt
    14041 ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa aagacgtatg
    14101 ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat gataagtgaa
    14161 agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt attttacatc
    14221 tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc agatggacag
    14281 caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa aagaaaagct
    14341 cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg gttgtcttct
    14401 gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg tacttttgaa
    14461 acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa aaccgtcaga
    14521 cgtggattat accttttcag cggaccagtc ggatcaggca agacttctct aatgtatcgg
    14581 ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga gatagaagaa
    14641 ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga tacacttttg
    14701 agactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag agatgaatca
    14761 actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc aaccgtacat
    14821 gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa aaaagaagag
    14881 ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc caatcaccat
    14941 ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa atcatgggag
    15001 gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta aaggattaac
    15061 aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat ctggttttag
    15121 tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg ttattttggc
    15181 aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt cgcaaatagg
    15241 atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga atctgccgac
    15301 aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca atgatttgaa
    15361 aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct tgtttagtat
    15421 gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag agcattgggg
    15481 tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag cggttactct
    15541 attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc agagtgaatg
    15601 gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg cttatttgtc
    15661 actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca tagtctgctt
    15721 aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga ttcaaggatt
    15781 ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat cagaagaatt
    15841 cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac tgatttttta
    15901 cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc attttattca
    15961 gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg ctgttttatt
    16021 acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat atcttattat
    16081 agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt attagtgttg
    16141 ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag tcgtgaagcg
    16201 atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa tggaagagtt
    16261 cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc agaaatctat
    16321 cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat cactcttact
    16381 gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg catggaagaa
    16441 tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt ctacgcaaaa
    16501 aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag agaatcaaat
    16561 catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata ttcctgatga
    16621 gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga atgaaagttc
    16681 actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt atcaattcca
    16741 gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt ttgttggagt
    16801 cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt ggccagctgt
    16861 ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga atgatttatg
    16921 aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa gaaatgatcc
    16981 catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt gtcaatatgg
    17041 agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct gattgaatgt
    17101 ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt gattcagcaa
    17161 accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct aatattttta
    17221 gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa tcagcaactg
    17281 cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat gatacgtaaa
    17341 agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct tcaattattg
    17401 gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga tggatatgcc
    17461 atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat tttctgtctt
    17521 gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct accgtttgtc
    17581 aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga agcagatggc
    17641 tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact acacaactgg
    17701 atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag tctcaccgtt
    17761 tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag aatgatggaa
    17821 tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa aatgtttgcc
    17881 gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc gatagaatta
    17941 aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt taccagaaaa
    18001 aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga atgcattggg
    18061 aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg attatcaagt
    18121 acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag ctctttatga
    18181 agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc aattactttt
    18241 attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc ctgatagtat
    18301 cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag caccggttaa
    18361 gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt taaacctatc
    18421 gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat tgttggctgt
    18481 cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc aagacgggtt
    18541 gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa tcggttatta
    18601 tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc atagttatgc
    18661 acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt ttggtttgtt
    18721 tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa aatggtttaa
    18781 agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc gtaataaaca
    18841 atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac aagtaaaaga
    18901 agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg aatttttcaa
    18961 cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg agagtcatat
    19021 gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat tatatcaaat
    19081 gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac aagattctat
    19141 ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata tcgaaaacca
    19201 cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt taagctctta
    19261 tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt atggggattc
    19321 agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat ttgctccttt
    19381 gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac ctgacatttt
    19441 gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa attatttata
    19501 tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct tccatggcac
    19561 aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga tcgaagaact
    19621 aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg atggaggtaa
    19681 atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg gtacacgttc
    19741 aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga ttgatattga
    19801 tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag gattgtctag
    19861 tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag cttatgacat
    19921 tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga atggtgttga
    19981 tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta gtgaaatcat
    20041 caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat tgcgtggtga
    20101 agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc caacagatga
    20161 ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag cattcgttag
    20221 gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt gcggagaaga
    20281 ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt cttagggaat
    20341 tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg attttcagga
    20401 aaatcccaat attttatttt cattcatctt tataagggac attacatatg taaaaagaaa
    20461 aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta tgtttagcta
    20521 gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc cacctgtact
    20581 ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga aaaaacgtcc
    20641 tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat aacgagtgct
    20701 tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag cgttccttga
    20761 tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg attggttggt
    20821 tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc caaacggatt
    20881 ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata aaagccactt
    20941 ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc gtaagccagc
    21001 aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat ttgcaagcta
    21061 ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa tttgagtaag
    21121 gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg atagactgca
    21181 aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt ttgaatgatg
    21241 aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt tttcggggga
    21301 ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt ggcttttttg
    21361 aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat catgactttc
    21421 aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc caagcgagct
    21481 tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc accttcttcc
    21541 agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga aacgaccaga
    21601 accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt aagatctaaa
    21661 tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat tttggctaaa
    21721 gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg acggtgtgga
    21781 atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc gcctagttct
    21841 tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt tgtatctggt
    21901 tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat ctcagcggca
    21961 gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg ttcgaccgtc
    22021 ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc tctgccggtc
    22081 aagatttgta aaaaggttga tttgccagtt ccgttgatac cgactaaggc aattttttca
    22141 ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat tgttttttta
    22201 atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa aggccatggt
    22261 tatccatggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct tgagatggcc
    22321 attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca gcaactgtaa
    22381 aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta cttgttattt
    22441 tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc agtgtacctg
    22501 aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga tgtgtaattc
    22561 tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca tcattgtatt
    22621 ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac tttgtctaca
    22681 tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct ctttggaaat
    22741 ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg tagcctttca
    22801 tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc cgaacccgaa
    22861 ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat attttttcgt
    22921 atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat gcttaggtcg
    22981 ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttta ttttacttga
    23041 actcattgac tgttttccct ttattttaaa cattataaag accgatgagt gcttgccgcg
    23101 tcttttgttt ttatcacggt tttttgtgca aaaaaacctg ccgatagttg gcaggtcgtc
    23161 tacgtgttaa gctttggtgt ggtctttagc tggcaattgg ttttctaaat cagttcggac
    23221 aacaagcaca tcgcaagctg cgtttctaat aacatattca gaaacagaac caataaataa
    23281 acgttctaca gcattcaatc ctgtggctcc taacataatc aaatctactt gattatcttc
    23341 tgggatttct ctggcaataa tttgttttgg tgagccatat tctacgacag aggttacatt
    23401 attcaagcca gctttcttcg cattgctttc ataatctgcc aatgtttgct ttgccatttc
    23461 agtagcttgt tctgcaagca ttccatcaaa ggatgaaacg gtttggaaag aacgtgtgtc
    23521 aatgacatgt gctaatagaa gttctccgtt atttcggata gctacattga ccgccttttt
    23581 aaaagctaat tcagcttcat cagaaccatc tacggcaacc atgatttttt tgtattgttg
    23641 taacataagc attcactcct caagtcaatc gtattgtact cttttattgt agaacacttt
    23701 agggaaaaag agaattattt actgctttgg ttcagcaatt ttttttgcaa atcggatact
    23761 gaaggtggca gaaacaataa ttacgatcag taaaaagacc aaggcaataa agcgttgatt
    23821 gaaaaaagct gttaaaatac ccattacgcc taaaacagca tatgcagctc cgtaaaattg
    23881 taggaatcct tgatttttat catttttgtt gatcaataca aaaaagatat cctgtttatt
    23941 aaataaataa tagctaacga ataataatac taaagtggcg ataatcatta aaatcctaat
    24001 catgaacaaa tcctccaaaa taagcaacaa atagaaaaag caatttgcca agagcaaatt
    24061 gctttattct ttaaaagatg gtaatctccg ttaatgccgt tgtttgtcct gtagtattga
    24121 tcccgcgaat tcaagcaggt gggtcccatg gtcgtagctt cgaactacca aatgataagg
    24181 catgttacca gcagtgacac aagacgggtc ccaagatatc aataaaaatg ttcggtcaac
    24241 acacaaagag gacaattcga acatcacaga tttcccacct ctatagtagc atatgaattt
    24301 aaaaagagca atgagagagc tcgcagaaac agaaccgttt tcttgtctag ttttttggag
    24361 gatgcttttt ccactctttt atacggtaat actgttgacc cagagcctgt tcatatttcc
    24421 ctgtatcttt tggctgataa tactgagcat tcttcagttt atctggtaaa tattgctgat
    24481 tgacccaagc ctgatcaaaa tgatgaggat actgataacc cacgccacgg ttgagtgatt
    24541 tcgcaccttt gtaatggcta tctctcaaat gatcaggaac atctccagct ttgccctctc
    24601 gaatatcagc aagtgctgca tctaatgcca tgtaggcaga attggatttt ggcgacaagc
    24661 aaagatcgac gacgacgtct gctagtggga tacgtgcctc aggcaatccc aatttttcag
    24721 cagctaatac agcgttcact gtacgagccg cagcagcagg attacctaaa cctatatctt
    24781 cataaccgat aaccatcaga cgccggcaaa tacttgccag atcacctgcc tcaactaaac
    24841 gggcgagata atgaagtgca gcatccacat cacttccacg aatcgatttt tgaaaagccg
    24901 aaataacgtc ataatgagca tctccatttt tgtcatgggt aagagctttt ctttggacac
    24961 attcttcaat gatcgacagt gtaagatgaa tccttccttc tttatctgaa ggagtcgata
    25021 gtgtagccag ttccagccca ttcaatgcac tccgcaaatc gccgttcgtt gcacgcgaaa
    25081 ggtgaagaag agcatcttcg tctaactgga ttgcttgttg tccaaggccg cgttctttat
    25141 cccttaaagc atgttcgact gccaactgga tgtcttgttc tgttaatggt ttgacttcaa
    25201 agatctgtgt acggctgcgg atcgctggat tgatggtaat ataaggattc tcagtagtcg
    25261 ctccgatcaa gatgattcgg ccactttcta aatgaggaag cagaaaatcc tgttttgttt
    25321 tatctaatcg gtgtacttca tccaataaga ggatcactgt accactcatt tttgcttctt
    25381 ccgctacaac ttgtagatcc ttttttgaat ctgtagcggc gtttagcatg cgaaatgcat
    25441 aattagtaga gccggcgatc gcacttgcga tactcgtttt tccagtacca ggaggtccat
    25501 atagaatcat ggaagagagc atgcgagcat cgaccatgcg gcgtatgatt tttccttccc
    25561 cgactagatg ctgctgacca acgacttcat cgatcgttcg aggtctcatg cgataagcca
    25621 atggctgttg cataggattt tcctccttct tgtttttacg tcccttaatt atatcatatt
    25681 tagcaagatg cctagaaaga acagccttct gttgttttgt tttcatagct tatccaacta
    25741 atattgtgta tgataaagta ggtgagaaaa atgacggata aacaaaagat agatgaattt
    25801 ttttggaata aaacgacgcc agaagtggca cgggatttat tggggatgta tttggagcat
    25861 gacacaccag aaggaagatt agctggatat attgtagacg cggaagcata cctaggtcca
    25921 gaagacgagg ccgcccatag ctatggttta agaagaacac cgcgtgtgcg ggcaatgtat
    25981 gaaaaaccag gtacgatcta cctttacacg atgcatactc atcggatttt gaacatcatt
    26041 actcagccag aagggattcc tcaaggagta atgatacgtg caatcgaacc cgccgcaatg
    26101 attgatcaaa tgagtaaaaa tcgtggcgga aaaactggtc cagatatcag caacggtccg
    26161 ggaaaattag tggaagcatt agcaattcct caagaacttt atggtcagtc tatagcagat
    26221 agttcgttac gcttagtttt tgagaaaaaa aagacaccaa aaaagatcaa tgctttgcct
    26281 agaatcggta tcccaaacaa aggtgtatgg acagaaaagc cgttgcgttt tgttgtgtcg
    26341 ggaaatcctt atatctcttt acaaagaaaa aaccaaatcg agaaagattg gggttggagg
    26401 aaagaaaatg aaaaagaagg atctattaac atatttagat gaaataattg aaaaacaagt
    26461 gacagactat gatgtagctt tagattggga tacaaaaaat catacgatcg agatcgttgt
    26521 tcgcttgttt gcagaaaata aagcacaatt tgagattgat gatgcagaag ggatcgtttc
    26581 gcaagaacca attatcgaat ttgaagacgg cgtacttctt tttaatcctc agaaatctgt
    26641 ttttgatgaa cgcaattatc tagcagtaat cccttatgaa ggaaaaaaag ggttggcaaa
    26701 atcagtagct gattctttag tggaatatct aaatgaagta ttagctcaag ggcaaagcga
    26761 tttgcttgat tttttggatg aagacgatga cgaagctgta ttcgagcttc actgggataa
    26821 tcagcaacta gcagaattag tagaagtaaa acagcaaaat gaagcagata cttatttacc
    26881 gtacccaagt tattaaggag tgacagatat gaaatggaat gaagtaaaag tagaaactga
    26941 aagtgaagca gtcgaagcag ttgctaatat tttgatggag gcaggggcga gtggtgtcgc
    27001 gattgaagat tctttagatg ttgaacattt tcagtcggat caatatggtg agattttaga
    27061 taaaaatgat tttacatcgc tgaaagaagg cgcactagtc atggcttatt ttccggaaac
    27121 gactttctta ccggaaatct tgccttttat caaagatcgt atcacacaat tacctgagtt
    27181 tggtctagct attggaaaaa ataaagtaac agttagtgaa gtagaggaaa gcaactgggc
    27241 aacagcctgg aagaaatact atcatccggt gcgtattacg cgaatgctga cggttgcgcc
    27301 aagctgggaa aactaccaga cagaagatcc tctagaaaaa attatcacac tagatcctgg
    27361 tatggcattt ggcacaggca ctcatcccac gacttccttg acattgacag cactagaaac
    27421 agtcttgcgt ggtggtgaaa cattgctaga tgtgggaact gggtcgggcg tgctaagtat
    27481 tgcagcaaaa tatcttggtg caaaagatgt ctatgccttt gatttagacg aagtagccgt
    27541 tcgttcggcg aaagaaaata tggatatgaa tgaaatagcc aaagatgtcc atgtatcagc
    27601 taacgatctt ttgacaggaa tcgagattga aagtgatgta atcgttgcga acattttagc
    27661 agatatcatc ttattgatga tcccagatgc ttggcgttta ctgaagcaaa caggcacgct
    27721 gatcgtttca ggaatcattg aagagaagaa gcaaatggtt ctggatgcca tgttagaaca
    27781 agggtttatc gtggatcaaa ttttccagca aaaagactgg tatgcaatta ttttgaaaaa
    27841 accagaggtg gaatagatgc agcggtattt tttaaaagaa tcatatgaat caaaagacta
    27901 ttttgtttta gaaggggaaa actatcacca cattgtacgg gttatgagga tggaacccga
    27961 ggatgaagtt tttttggcat tcaatgatca aatagccatt gttgcagaaa tcacggagat
    28021 tactgaagag gctgtttatc tgaaagaaat taaaaaagaa gaaaatcaaa aggaactgcc
    28081 aagttcgatt accatagcga gtggctatcc taaaggggaa aagctggatt ggatcgttca
    28141 gaaatccact gaattaggag cgcaagcttt tcttggtttc ccggcatcag cctctgtcgt
    28201 taaatgggat gcaaaaaaac gtatgaaacg acaacaacgg cttgaaaaga ttgctcaaga
    28261 ggcagcggaa caatcccatc ggcagatcgt gccatcagta gagttattgg aaacgaaaca
    28321 agatttattt gagcggctag atgattatga cgtggtcttg gttgcttatg aggaatcagc
    28381 aaagcaaggc gaaaaatcaa atgcagccaa aattcttagt gaaacaaaac cagatagtcg
    28441 aatcttagct gtctttggac cagaaggtgg attatcccgc gatgaagtcg attattttgt
    28501 agaacacaaa gggatacttt gtggattagg accaagaatc cttcgtacag aaacagcacc
    28561 tctttattta ttaagtgctg ctagctatca atgggaatta atgaatcctt ctttcagcta
    28621 acgttcttca ttaataaaag agtatcgaca gaaagaattg aaaaaatagt caacaatttg
    28681 tataatagaa acaattattt atacaatgga tacgaaaaat cgtttgaaaa agcttttaaa
    28741 aagcgaacat ttccgtaaaa aaacgtataa ctgcacggat tttgggacaa ttatcaaaca
    28801 gatatttgtc ccgattcccg ttttaccgaa aaaagaaagg gggatcgatt cctgtgacaa
    28861 aagaagaaat tttaacggga cccggtgtca taaagctcgt tagtcaatac atgggtcccg
    28921 aacatgtagc atttgttgag aaagcatgcg aatacgcgac tgctgcacat gacggacagt
    28981 ttagaaaatc aggcgaaccc tacattattc atcctatcca agtcgcaggt atattagcag
    29041 atttaaaaat ggatccccat acagtggcta caggcttctt acatgatgtt gttgaagata
    29101 cagaaatcac tttagaagat ctgagagaag aatttggcga tgacgttgct atgttagtgg
    29161 acggcgtaac caaattaggg aaaataaaat ataaatccca cgaagagcag ctagcagaaa
    29221 accaccgaaa gatgctgcta gcaatggctc aagatttacg agttatcatg gtcaaattag
    29281 ctgatagact acataacatg cgtacgttga agcacctgcg agaagataag caaagaagga
    29341 tcgctcagga aactttagaa atctatgcgc ctcttgcaca tcgtctaggg atcagccgga
    29401 tcaaatggga attagaagat acagcacttc gttatctaaa tccaaaacag tattaccgta
    29461 tcgtccactt gatgcagacg aagagagaag aacgtgaaaa atacgtaagt ggtactgttg
    29521 aagatattcg aatagcgaca gaagagctgg ggatttttgc agaaatctat ggacggccaa
    29581 aacacattta ttcaatctat cgtaaaatga aagatcagaa aaagcagttc aacgaaattt
    29641 atgacttgct agctattcga gtgatcgtag attcaatcaa ggattgttat gctgtattag
    29701 gagcaatcca cacaaaatgg aaaccaatgc ccggcagatt taaggattat attgccatgc
    29761 caaaagctaa tatgtaccaa tctttgcata ctaccgtgat cggtccggca ggaaatccgg
    29821 tagaaattca aatccgaaca caggaaatgc atgaaatcgc tgaattcggg gttgctgcac
    29881 actgggccta taaagaagga aaaaatgaaa aagtagaacc agatggtatg acgaaacaat
    29941 taagctggtt ccatgagata ctcgaacttc aagacgaaag ctatgatgct tctgaattta
    30001 tggaaggcgt aaaaggagat atctttagtg ataaagtcta cgtcttcaca ccaaaaggag
    30061 acgttactga gttaccaaaa ggatccggac cattggactt tgcatacagt atccatacag
    30121 atatcggtaa caaaaccact ggtgcaaaag taaatggcaa aatggtgcag cttgattaca
    30181 aattgaaaaa cggagatatc attgagatca tgacttctcc aaattcattt ggcccaagtc
    30241 gcgactggtt gaaattagtt gctactagca aagcaagaaa taagatcaaa cgtttcttca
    30301 aagcccaaga tcgagaagaa aatgtgatca aaggccacga atccgtggtc aaatgtatta
    30361 cagatctagg atttacgcct aaagatattt tgacgaagaa caaactgcaa gaagcactcg
    30421 atcgttttaa ttatcaaaca gaagatgatc tctatgcagc tgtagggtat ggagaagtta
    30481 gccccttgac gatggccaat cgtctgactg aaaaagaacg taaagaacaa aaaatcgagc
    30541 agcaaaagca agaagcagaa gaaatcatga atcagccgaa aaaagaacct gacaaaatga
    30601 aagtacgtca tgaaggtggc gttgtcattc aaggggtaga aaacttacta attcgtatca
    30661 gtcgctgctg taatccgatt cctggtgatg atatcgttgg ttatatcact aaaggcagag
    30721 ggatatccat tcatcgtcga gattgtccga atgttcagcc tgacaaacca aatgtagcag
    30781 aacgtttgat tgaagtcgaa tgggaagata catcgaatac acgaaaagag tatgatgcag
    30841 atttggaaat ttacggctat aatcgttcag gcttattgaa tgatgtactt caaacagtca
    30901 atgcgctaac gaaaaatctc aacagcgttg aagcacggac gaataaagat aaaatggcga
    30961 cgatccattt gacggttggt atccagaatt tatcccacct aaagagtatc gtggataaaa
    31021 tcaaagcagt acctgatgtc tacagtgtac gccggacgaa tggataggag gacagatatg
    31081 agagcagttg ttcaacgggt aagtaaagct gctgtgtcag tcgatcagca agaaattgga
    31141 aaaattggct acggcctact tgttttatta ggcattcatg aaaccgatac gcaaaaagat
    31201 gtggactatc tggtaaaaaa aattgcacaa atgcgtattt ttgaagatga acaaggaaaa
    31261 atgaatatta gtgtagaaga tgtgggagga caaattttaa gtatctctca attcacactg
    31321 tttgcggata cgaaaaaagg aaatcgacca agctttgtgg aagccgcacg accagaaaca
    31381 gctattcctt tatacgaatc attcaatgaa ggaatcagaa gtcgcggcat cacagtcgct
    31441 accggacaat tcggagcaga tatgtcggtt tctctcgtca atgatggacc tgtaacgatc
    31501 attattgatt ctaaaaacaa ataaagtaat ctggttttca ctagtaaaaa ctatgaaaca
    31561 cgtcgcttaa gttagactga taaactctaa cttaagtggc ttttttgtct gcaaaaagcg
    31621 cttcttttgt cagcagtcgg aaaaaaagtt aagcttaaat tatacaagtt attttttata
    31681 ggaggttgct gaagatgaaa gaagtaccag tagtagatat caaaatcaca gatttaaaaa
    31741 aattgtatca aaaaggatac aacatgttgg aggagttacg ccatgaagcg gacgctcccg
    31801 tcgtcaaagc aaagattttc aataaagaag ctatcacgat ctacggatca tcagcagcaa
    31861 aagtatttta tgatccaagg aatttcaaac gaaaaggtgc tatgccaaaa ttagtattga
    31921 agacactttt tggtcaaggt ggagtacaga ctttggatgg agctgctcat caccatcgga
    31981 aaaatatttt tatggatcta atgacaccgg aaagaatgga agattatcat cgtatcttag
    32041 ataagaatct gacacaagca cttgaagcac agcatggtca atttgaacta tttgatttaa
    32101 gtaagatggt tttctttaca tctatttgcg agtgggcagg tatcaattta tcagcgattt
    32161 ctaaagatga ggtcgaaaaa ttagcagaat atcaaatctc tatgatcagc ggaacgttta
    32221 cttctccgat tgatcacata aaaggtgtcg aaaatcgcaa aaaatcagaa aaatgggcgc
    32281 aaggcttgat tgaagaagct cgacaaaatc ctgtggctgg aaaagaaaat gtggcattat
    32341 atgcttttgc caatgccaca gatttagatg gtcagctttt acctctggaa gtggcagctg
    32401 ttgaattatt gaacatcatc cgtccaacgg ttgctctaac tgtgtgggcg gcattgatgg
    32461 gacatgcact tttcagtaga ccagatttgt atcagcaatt aaaaaatgac ttttcgacgt
    32521 tacaagatcc gtttattcaa gaaatgagac gctattatcc tttcttccca atgctacctg
    32581 cgatttcgct gaaagaagta gaagtagatg gttatcgtat accagaaggc agttgggtta
    32641 ttttagattt atatggaaca gaccatgatg aacgaacagt tgaagcgcca gattccttta
    32701 tgatcaaacg gtatgtaggg aaagcaaaag atatttccta taaagaagaa tatgagatga
    32761 ttgcacaagg cgggggaaat tttcgacaaa tgcatcgctg tgcaggagaa tggatcactc
    32821 ttcatagtct acgtgtgttt tccgatcagc tagtaaataa gtttgagttc tctgtacctg
    32881 aacaagattg gacgattcca ttcaatcagt ttccgactta tccgaatagc cgagcgctac
    32941 tttacaagaa ttagattagc ataaacttat cgaaaaaaag gaagatcaaa cagtgctgat
    33001 cttccttttt tcatattact ataaattgat aaggtcaatt gatcaaaatc cacgaaatgc
    33061 ttcatcaatg gtttgataat cttcattgga taaatgcaaa tctaaagctt ggacattgtc
    33121 agatacttgc tctggacgct tagctccagg tataactaca gacactcgtg gattcttcat
    33181 gtaccaagct aaaacaagtt gcgcaggagt ggcttggtat cttttagcaa tggggcgaag
    33241 tacatcgaca gcggttaaaa tttctctgaa acgttctcct tggaaatctg gatttctttt
    33301 tcgaggatct ccttcaccaa attgtttttc ctcaccaagt tcatattttc ctgtcagcaa
    33361 accggaagca agaggaaaat agggaacaaa actgattttg tttttttcaa gataagggaa
    33421 taattctttt tcagcctgcc gatggatcaa actgtattta tcttctacta catcgacata
    33481 tccatctgca ttcgcttctt tcaattgttc taacgtgaag tttgacacac cgactgctcg
    33541 gatttttcct gcttctttta attcgtgcaa tgtagcaact gattcatttt ttggtgtgct
    33601 ttcatccgga aagtggatat gaaaaatgtc gatgtaatca gtttgcagac gttttagtgc
    33661 atcttccacc gattgtttga gaaactcagg agaattatca aatgttcgcc ctttatttgg
    33721 tacatgagca gcttttgtag cgatgacgac acgtgagcga tcatattctt tttccttcag
    33781 tacctcgcca atcagttctt cggaacggcc gtttccgtaa gcataagcgg tatcgatcaa
    33841 attgatcccg ctattcaaag ctgtacgaac tacttgtttt cctgtctcat cttccaaacc
    33901 gtcaaaaaga ttatgtcctc caacggcatt tgctcctaat ccaagctgtt ctgcgtagac
    33961 ttgtgaatgt ccgattctta tttctgccat agctgattcc tcctttttgt ctagtctaaa
    34021 acaaaatacc aaaaagctga actaaaacgt ttttgtgagg ggaaatagtc agatatctct
    34081 tgactttgag actggttttt agtaggttat aagtaatatc gaatgtcaat gaaagagcga
    34141 gtaagttttt gaaactttac aagagaggat catcgaggct gaaaatgatc taaagtttca
    34201 ttagcctaaa gacactctgg agatggacga gtgaaaagcc gtcccgcgta cgagcgttat
    34261 tcgtaagagg aaagagaaat ctttcaaaat taggtggtac cgtgtatgaa tttcattcac
    34321 ccttgttcat gatgaacaag ggtgattttt ttatttacat agctaagaaa gggagagaaa
    34381 actatgagtt tccaaagacc aaaaggaaca aatgatattt tgccaggcga atcagaaaaa
    34441 tggcagtttg tcgaagaaac agcacgttta ttattcagag attatcagta taatgaaatg
    34501 cgtacaccga tttttgaaca ctatgaagtg atatcccgaa gtgtagggga tacaacggat
    34561 atcgtttcaa aagagatgta tgatttttat gacaaagggg aacgccatgt gacgcttcga
    34621 ccagaaggta cagcaccaat cgttcgttct tttgttgagc ataaattgtt cggcccagag
    34681 tatgcgaaac cgttcaaaac tttttatatg ggaccaatgt ttcgctacga acgccctcaa
    34741 aaaggacggt tacgtcaatt ccatcaaatc ggagtagaag catttggatc agaaaaccct
    34801 gcaactgatg tggagagcat ggtgatggca cttgatttct tcaaacagtt ggggatcacg
    34861 caaatccggc tagtcatcaa ttcattaggt gataaagaaa cgcgtcaagc ctatcgtcaa
    34921 gcattgatcg actatctttt accttttgaa tcagagctga gtgaagactc taaacgccgc
    34981 cttcatgaaa atccattacg cgtattggac agtaaagaca aacgagatca aaaatttgtg
    35041 gcagaagctc catctatctt agattatcta agcgagcctg ctcaaaaaca ttttgatacc
    35101 gttgtttcca tgctggatgc attggctgtt ccttatgaaa tcgacagtaa tatggttcgc
    35161 ggactagatt attacacaca tacgattttt gaaatcatga gtgacgcacc aaaaatgggg
    35221 gcacaagcaa cgatttgcgc aggcggacgt tatgacaatc tagtggaaga actaggtggt
    35281 ccttcaacac ctggcttcgg atttgcaatg ggtatcgagc gattactctt gactatggag
    35341 gcagaggaag tagttatccc tgcatttaat gagttggatg cttacgtggt cgcactcgga
    35401 gacgaaacaa atatcgaagc attgaaagtg gtccaagcga ttcgaaactt tggtttttca
    35461 gctgaccgcg acttcatgaa ccgtaaagca aaagcgcaat ttaagacagc agataaagcg
    35521 aatgccaaat tagtattggt gatcggtgaa gatgagcttg ctagtcaaac gattaatgtc
    35581 aaagcaatgg caagccgaaa agaaaaaagt tttccattag aagagatata tgaacatttt
    35641 gatgaagtct atgatgaaat gactttattc gagtaggagg aaaaaaataa atggcacaaa
    35701 gaacgatcta ttgcggatta gtttccgaac aacaattaga acaaacagtt acattaaaag
    35761 gatgggtaca aaaaagacga gaccttgggg gagtaatctt tatcgaccta cgtgatcgtg
    35821 aaggaatcgt tcaaatcgtc tttaatcctg aaatcaacaa agaagcatgg gaaatcgcgg
    35881 ataaatgtcg cagtgaatac gtgatcgaag tgactgggga agttagaaga cgcggagaaa
    35941 atgcagtaaa tcctaaaatg gccactggtg aattcgaagt gattgcaagt gaaatcgtga
    36001 ttttgaatca agcaaaaacc ccgccatttc aaattgaaga tgatcaagca atcagtgacg
    36061 aaatacgttt gaaatatcgt tatttggatc tgcgtcgtcc aaaaatgact aaaaacatca
    36121 aattaagaaa tgcgacaaca aaagcgattc gtcattattt agatgataat gactttctcg
    36181 atatcgaaac accttaccta gggaaatcta caccagaagg cgcaagagac tacctagttc
    36241 cttcacgtgt ccatgctgga catttctatg cgttgccgca atctcctcaa ttgtttaaac
    36301 aattgctgat gaatgctgga ttcgaccgtt attatcaaat cgttcgttgt ttccgcgatg
    36361 aagatttacg tggagaccgt cagcctgaat ttacgcaagt agatattgaa acaacgttct
    36421 taacagcaga agaaatccaa acttacacag agggattgat tgcgaaggta atgaaagaag
    36481 ttcgcggtat cgaagtcaca ttgccatttc ctcgtatgac ctatgatgaa gcaatggctc
    36541 gctacggcag cgacaaaccg gatacacgtt ttgatatgga attgatcgat ctaagcgata
    36601 cagtcaaaga agtagaattc aaagtcttcc aaatggctct tgaaaacggc ggtgtcgtga
    36661 aagcattgaa tgctaaaggc gctgcagatc gctactcacg aaaagacatg gatcagttag
    36721 gtcaatatgt aggacaattc ggagctaagg ggcttgcatg gctgaaagta gaagaagacg
    36781 gattgaaagg tccaattgct aaattcatgg gagaagcaac agaagcaatc attaaagcaa
    36841 cagatgcaaa accaggtgac ttgctgatgt ttggcgcaga taaaagtgaa atcgtcgctg
    36901 ctgcattagg tgctatccgg acacgtttag gaaaagaatt aggactaatt gatgaatcga
    36961 aattcaattt cttatgggta actgattggc cacaatttga attttcagaa gaagaaggtc
    37021 gatatgtctc tgctcaccat ccattcacga tgccaaaaga agaagatatc ccgttattgg
    37081 ctgaagaccc tgcaaaagtc tatgcagaag cttatgatat cgtcttgaac ggttatgaac
    37141 taggcggagg ttctatccgt attcatactc gtgagcttca agaaaaagta ctagaaactc
    37201 taggctttac aaaagaacaa atggaagaac aatttggttt cttgttaaca gctttagatt
    37261 acggattccc tccacatggc ggtattgcat taggattgga ccgttttgca atgttattag
    37321 ccggagaaga taatattcgt gaagtcattg cctttcctaa aaacggaaaa gcagcagatg
    37381 tgatgagtga tgcaccaagt atggtttcgc ctttacaatt gttcgaattg aatattgatg
    37441 taacgaatat agaagaatag ccgataaaaa ggtaaaaagg atttgtcgaa tacgcttctt
    37501 ttgacaacct tgatttaatt gaaaaacact ttataatgaa gccataacat ataaagcggg
    37561 gcttttccat gaaaaaaaca ttcgaaaatt gggcttatca tgattacacg accaagcttt
    37621 cggtatctat cgtttatgcg ttgatcgcat cagtcgcatt gaatttcttt tatcagccag
    37681 gacatattta ttcaagtggt attactggtc tagctcagat tttgacgact ttgtcaaaat
    37741 cactgatagg attcgaagta ccggtatcaa caactttgta tttactgaat atacctttgt
    37801 ttgtattggc gtggttaaaa atcgggaaga agtttacaat tttcactttt atcacagtca
    37861 ctttgacctc cctttgtatc catattttac cggaaacagt cctatctacc gatccaatca
    37921 tctgtgcgtt gtttgggggg gctgtgatgg gactaggtat tgggctagcg ttgagaaacg
    37981 gcctatcatc gggcggtcta gatatcgtga gtatcacgat tcgtaaaaaa acaggaaagt
    38041 cgattggttc tatttcgatc tacttcaatg cattgatcgt ttttgttgcc ggaattctat
    38101 ttggctggca atatatgttt tacagtacgt tgtctatttt cgtcagtgga aaagtaactg
    38161 atgtcgttta tactaaacaa aagaagatgc aggtaatgat cgtaacgaaa catccagaaa
    38221 aagtagtaaa tgaaatccaa aactgtatgc gtagagggat cacgatcatc aatgaagcag
    38281 aaggtgctta taagcatgat aagcagacag tattgatgac gatcgttaca cgatacgaac
    38341 tgcctctact tagagaagca atgaaagctt ctgatccaca agcttttgta agtattgcag
    38401 aaaacgtcca gatacttggt cgtttctatg aggatggcat ttagatcgag tttgacaaaa
    38461 attcatgaaa tactgctgta gacagacgtt cgtttgtctg cagttttttt ctaccatagt
    38521 agaatcagca aagaaaaact gcttttttat ttgaaatttt cttaaagttg aaaaaaagca
    38581 tgtttatttt tttaaattta tgggatgata gaaaaaaata gtagagaaag gacacgattc
    38641 tacttgctat tttcacttaa tacccgtata ttacgaatga aaaaagttta tgaaaagttg
    38701 gaatattgaa tggaaaaaca aaaaaggtta aaaaacagcc gctacatcac tgggtttgat
    38761 gggctccgtt cattagctgt ggtgggtgtt atcttctatc accttctgcc aactagtatg
    38821 aaaggcggct atttgggcgt tccgattttt ttcgtcgtat ctggttattt gatcacggat
    38881 ctgctgcggc aggaatggga acaaaatgga aaaatcaata tttggcaatt ttatgtcaga
    38941 agaatgaaga gactctatcc ggcacttgtt tttatgcttg tgacagcttc ggcttatatc
    39001 actttgtttc agcgagggct gttaaacaat cttcgaggag ttgtggtcag cagcttactg
    39061 tatgtcaaca actggtggca aatcaataac ggtttatctt atttcgatcg atttgctaat
    39121 gagtcaccgt ttacgcatat ctggtcttta gcagtagaag gtcaaaacta tttggtttgg
    39181 ccgatcttgt ttgtattgtt gatggtattt gttcgtaaaa gaaaatggat cttttatact
    39241 gtgttaggta catccttcct ctccgccatc ttgatgatga tcttgtatac accaggaggc
    39301 gatccaaccc gtgtttatta cggtaccgac actcgtctgt tttctatatg gttgggaagt
    39361 gcgcttgcgt ttgtttggcc aagtacgcgt ttaaagaaaa acatcccgaa acaagcgaaa
    39421 aatattctga atatcgctgg aggaatctcg cttttggcac tgattctttg cttcttcttt
    39481 ttagatgatc attacaattt catctattac ggaggaatgc tcctagtgag tctgttatgc
    39541 gtagtgctag ttgcagtgac agcacacccc ggagcaagtt tgaatcgatg gctgacaaat
    39601 ccagtattta cttggattgg caaacgaagc tatggcattt atctgtatca gtttccagtt
    39661 atgatttttt atgaagcaaa ggtgactgat attgccgatc atgttttgtt gcatacttta
    39721 gtggaaatca cattgattct aatcatcagt gaactttctt atcgatatgt ggagcgtccg
    39781 ctagcacgtt ttgattatgg tcgtacattc caagtgatga aaggatggtt cagcaaaccg
    39841 atcatcagta agagcaaacc atgggttgtt ccaggcacat tacttgtagc aattgcactc
    39901 gttggtttcg ttacggcacc gaaaaacgct gtaacagccg atcaaaaaca actgcaggca
    39961 aaaatcgctg aaagtaaaaa attagcagaa gaaaccagaa acaattcaga aaacaccact
    40021 agttcggttg accaagcgat ccttgataag tacgagttgt ccaaggcaca agggaataag
    40081 gcacaaaaat tagaactaac ggcatttggt gattcagtta tgttggatgc tgcaacggat
    40141 ctacaagaac tttatccaaa agtagtggta gatggagatg tcggacgaca attatatgcc
    40201 agtgtaccgt acattgaaaa attaaaggaa caagatttgt tgaaagacac tgttctgatc
    40261 ggactaggaa cgaatggctc attctcggaa gctcagtttg atagtgtgat ggatgcaatc
    40321 ggtgatcgaa aagtatactg ggtcaatgtc cgagtaccga cgaagcgctg gcaaaatgac
    40381 gtgaatgcga tgcttgaaca gatgacgaaa aaatacgaca atatgacgct gattgattgg
    40441 tacagctaca gtaatgacca tagtgactgg ttttatgatg atcaagtcca tccaaatcct
    40501 gaagggatga cacattacat tcacttagtc agcgaagcct tgctaggagg aagttcaagt
    40561 acgaacagca gtgagacaaa cgtgacagaa tgatagctac aaaaaacttc aggcaaaaac
    40621 atttgttttt gcctgaagtt tttttatatt taccgtgttt ttaacattca tatagattca
    40681 ggaagcaaaa cggttgagtg tgtctgtaaa taacgttctt tttgttttcg ggtgagtttt
    40741 ttgaatgcag cttctgcttt tgttgcttcg cttcttgaag aaaatacttc tgcatggatc
    40801 atcttaacag gtcttctttt ttgaagacga gtatatttag caccagtacc attgttatgt
    40861 tcctctaatc gtctagaaag atcggttgta tatccaccat aaaatgtctg atctttacag
    40921 aataatacgt aaaaataatg ttcatttgcc atagagcatc tcacgaatct ctgcagtgta
    40981 ttctccatcc atattgtaag tgatcaatgg tggagcgact ttgaatccgt ctaatttgcc
    41041 ttctttgatt ccttcaacta acaaaatatt tgcctctttt ccaggttttg ggtaaacaaa
    41101 ttggatcctt ttaggtgcaa ttcgatagtc ttccatgacc tgaatgattt ctaagaaccg
    41161 atcaggacga tggaccatag taaagcgtcc atttgttttt agtagcttac ttgaaatatc
    41221 gatcacttct tctaacgtcg tatgaatctc atgacgggcg attgccaggt gtttattagg
    41281 gttcgtttta tttgttggca gccctttgaa ataaggtgga ttacagacga ctaagtcaca
    41341 agaattatgt cgaacgacag ataagctgtc tttcagatcg atggtgtgga cagtcatttg
    41401 ttcatttaaa tggttaagcg cgatactacg tcttgccatg tctgctagac gaggttgaag
    41461 ttcgatagcg tcgatagccg cctgggttcg tttacttaaa aaaagtccaa ctgcgccatt
    41521 tcctgcacaa aggtcaacaa tcttcccttt tttaggtaca cgggaaaagt gagcaagcag
    41581 cacagcatct aaagaaaaag aaaacacttt ggaactttgg ataatcttga tatcttctgc
    41641 gtataattgg tcgattcgtt cgtcttcgtg aagcatggat tccctccaaa atgaaaaaaa
    41701 tagtcgtctg tattatacta tttttattaa aaagaaccaa ggtggcttgt caaacctcta
    41761 ttttttatgc atactatgac cataagaagg gaaaggaaga gactatgttt tatcgattta
    41821 tgcgcggggt agttcgagta ctattagcct tagttaatgg aaatgcccgc tatgagaata
    41881 aagaagtttt accgcaagat gagaattaca tactagttgc tccccatcgt acttggtggg
    41941 acccattata tctggcggta gctgcagcac ccaaggaatt cagctttatg gcaaaagaag
    42001 aactgttcaa aaatccagtt ctgcgattta tattaactcg atccaatgct tttccagtta
    42061 aaagagaaaa tccaggacca agcgtcatta aaacacctgt aaagattttg aaatcgacaa
    42121 atttgagttt gatcatgttt cccagtggga caagacatgc tacagaactt aaaggcggaa
    42181 tggcattgat ttccagaatg tctaaagcga aaatcgtgcc agctgtatat caaggtccgt
    42241 tgacacttgg ggatctgttc aagcgtcaga gagtgacagt gcgtttcggc gaaccaatcg
    42301 acttatcaga tatcaagaaa acggataaag aaggtatggc ggagatcgaa cgacgaacac
    42361 aagcagcttt tgacaaattg gacaaagaag tgaaccctga tttcaaatat gaagtcaaaa
    42421 aaaaataaaa tgataagttt atgcgtgggc ggtcttttga atctactgtc ctgttcagtt
    42481 cagacttttt caatgaaaac gagggagaga cgtcaagtct gccctctttt tgcgttccaa
    42541 tatgtaaaat agaagacagg tgtcatgtaa tggatcaaag tgttataata attagaagga
    42601 cattttttga ggtgattgaa tgcgtttttt acatacagcc gattggcata tcggaaaaaa
    42661 actacaaggt tatgacttga ttgaagacca gcgtcatgta ctatcttcta tactagatat
    42721 cgcgaaagaa gaaaaagtag aggcaatcgt gattgccgga gacttatatg ataggagtgt
    42781 cccagctgtt gatgccgtag aattatacaa tgaattgatg gtcgattgga atttaaagga
    42841 aaagatcccg atttttgcta tatcaggaaa tcacgatagc agtacgagac tttcagcggg
    42901 tacgccatgg ttcagttatt cgaactattt tttgcgaaca cagctagcag aagcatttga
    42961 accagtcgag tttaaaaacg tacaattttt ctttcttcct tactttgaac caattgcagc
    43021 gagattatat tttgaagatg acaatatccg aacgatcaag caagcggtag ccaaggtgat
    43081 agataaaatg caggagctat ttgattcaga aaaaaaacaa gtattgatta gccatttttt
    43141 tgtaatggga agtgtaaaat ctgattcgga aacgaaaatc gaagtaggcg ggttggattc
    43201 gatccctggt gaactactta caccgtttga ttatgtagca ctaggacatt tgcataatca
    43261 agctgctttg agacaggata atgctcgcta tagcggttcc ccattgaaat tctctttatc
    43321 tgagatcaac caaaaaaaag gcgtatggat cgttgacctg acaacaggcc tggagttgaa
    43381 ttttagggag atcgaacccc tgcatgatat tgaagaaata acagcgagtt tcaaagaatt
    43441 gctggatcct gccttttatc agtccgtcaa tcgggagaat tacatctata tctatctcac
    43501 tgatagaacg atcatcccga atatgatgaa ccagcttcgg caaatatatc ctcgtatttt
    43561 ggggattgaa cgggcaaacg gtcgagcaga acaaacaaag cgcaaacgaa aagtactaga
    43621 aatgaaagat cctcaaaaat tagcagaagc attttttagt gaaatgacgg aagagcagct
    43681 aactgcaaac caagaaaaat ggctcggaga aacgttgcaa gaaatcatgg aaatggaatg
    43741 atcaagaatg aaaccgaaaa aattacaact taaaaatttt ggtcctttta tcaatgagac
    43801 cgtagatttt tcacgtttga cagaagcacc gctttttttg atcagcggga agacaggagc
    43861 tggcaaaacg acgatttttg atgggatcac cttcgcactt tttggtgaaa cttccggaag
    43921 acttcgctct ggaaaagaga tgcgttcttt gtttgctaca ccagacgaag aaacaagcgt
    43981 gactttttct tttgaacatc agcagatgag ctatgaaatc gaacgaaaac cagaacaagt
    44041 tttagcaaaa cgaaaaggaa acggaacaaa aaaacaggca gccaaagtaa cgttgaccat
    44101 ttttgatggg aaaggaaaag aaataaaaca aatcacaaaa cgtacagaag cagatcagtt
    44161 gatcagagaa ctgatgaatc tcgatgcgaa acagttttct caaatcgttt tactacccca
    44221 aggagaattc cgtaattttt tggtttcatc aagcagtgat aaagagactg ttttacgtca
    44281 tttattcggt actcaatttt tccaacagtt caatgagcga ttaaaagaaa aagcaaaaca
    44341 acagcaacaa aaattggatc atttagagca agagctgcat cttctgcaaa aaaggtttgt
    44401 tttagtcaaa gaagaggaac cagcagcagg gtttgaagcc gttttagccc aatggacaaa
    44461 ccatcaggca atactcgaaa aacaaatcac ggaagaaaaa gaagctctaa cggaacttca
    44521 ggaaaaacaa aagcaactag aaaattccta ctatcgtttt gaaagcttga aaaaaagtta
    44581 tgaagaaaaa acgatattaa taaacaagca aaaggaatta gctgctcaaa aagacgaaat
    44641 agaggaaaag aaacgatgga ttgggtatta tgaatttacg caacgattag tcgaaccaat
    44701 ccagcgccta caagaaataa aagttgaaaa aatagaactg tcacaaaaaa gtagtgagca
    44761 aacaaagaaa ctagcgatga ctacgcagaa ttatgaagag tggcaaaaaa atgaagcaaa
    44821 ccgcgtacgc cgacaaacaa aaatcaaaga agctgctcaa aaactgcaaa acaaacagat
    44881 gatggtgcca attgtcgaat ccttacgtag taaaaaacaa gacgcagaac aattaacaat
    44941 ttcattggat aaagaagaat tgaatgccga gaagctgtat caaaatctcg ctatctatca
    45001 ggaaagaact cgaattcttc aagaaaattt actggatcag gaaaaactgc aagaagatcg
    45061 tcttgctttt ggacagttaa aatatctaga tgaaaagcag caagaaatag aaaaacaaaa
    45121 agcaaaagac actgggtcgc tcgcacagat aagagaagaa ctcaaaaaag caactgaaga
    45181 aataacaaaa aatagtcagc tattgaaaga acagacagaa caagcagaac agctgaaaag
    45241 ccaatgggca aagcagcaaa ttgctcgtct tcagcttttt ttgcgtccag gggagccatg
    45301 tcttgtttgt ggatcaacgg aacaccccat acaacaatta aaacacgaac agccaaataa
    45361 ggaagatatc ttaaaagtag aacaacaact ggcagaagct gagtctgctg tagaaagaac
    45421 tcagcaaatc attgccaaaa gtgaagcaca tgaaaataat cttcaacaac aattatcaga
    45481 tttagaacaa aaaatcatgg caacgaatat acaattggct gaacaagaag aacacttgca
    45541 acaggctttg aaaaaggcat ttcctgagat aacagaacaa aaaacaaaaa caactcttgt
    45601 agtaatcgaa caatctttag atagacgaaa aagcaagatg gccaatgcaa gaatagagct
    45661 ggaagaaata agacaacaag cagaaaagct cgaacaacaa ttagctgtct acaaacagca
    45721 gcagacagaa aaaaataatc agctccagca actgcaagga gaaatcaaaa gtttagaaca
    45781 acaattgcaa gatacggata gtaactgttt agaagaggag atacgacgac ttgaaaaaca
    45841 gttagaagaa gactcgaatc aactagctgt tgagcagaaa gcaggagtgc aattgttgca
    45901 agatcttcat acgcttcgga cacagcttga tcttcaacat gaacaaatag aaaaaataga
    45961 tgaaaaaata gaaaaggaac aagaaagaat agctaaaaag ttagaaagcc agtcgtattt
    46021 ttcaacaatt gaagaaatct gtgcatttgc acaacagcaa gcacaatata aagaaaacca
    46081 acgtacagtt acttcttatc atgaagagca attagtgaca gctgaccggc tgcgtcaatt
    46141 agaaaatatc cagctaactg agacattccc agatacagaa gagatcagcc aacagttaga
    46201 agaaataaga cgtatgatcg aaataaagca agaaaagata tacggtttac aagaacaaaa
    46261 aattagtaat caaaaaatct acgatgaatg tctttcgatt tatcacacaa gccagacgaa
    46321 attagatgag cttacgcaat tacagcagct ttcgcaaaca ttcaacggag aaaatcctaa
    46381 gaagactagt ttggaacggt atgtgcttca ggtttatctt caagaagtgt tacaagtagc
    46441 aaatgatcat ctccaacgtt taacgaaaaa tcgttatcaa tttgagctag cggataccat
    46501 tggcagttat cgagggaaaa ccggattaga aatcaatatc tatgatgatc aggcaggtat
    46561 gtctcgaagt gcgcataccc tgtctggcgg tgagagtttt attgcagcac tttctttagc
    46621 attagctctt gccgatgtga tccagacaca agctggtggt gtagcaatcg atgcattgtt
    46681 tattgatgaa ggatttgggt cattagatga agaagctttg gaaatggcaa tggaagcttt
    46741 agaaacgatc gaaagtgaag gaagaatgat cggcatcatt agtcatgtaa gagaattgaa
    46801 agaacgagta ttacaacaaa taagaattga tacagaaggt agcggacaaa gcaaaatacg
    46861 ctatcaactt ggataaaaag ggagattggt aactatgaaa tggagtattc cagaaaaagt
    46921 cattgcacgt ggacgtcaat acttgcaaga aggccgagtg ttatcggttg tccctgacca
    46981 agaagaaaac gtgtggcatg cagaagtctt gggcagtgaa ttgtatatgg ttgatttgga
    47041 tggtacagca aaagagatag attactgcca atgtccctat tgggaggaac ataaatattg
    47101 taagcataca gttgctgtgg aactttattt acggtcgaaa aatctatctc gtatcatgaa
    47161 aaaagaccag ccaatgctag aaccggtaaa agcaacaagt gagggagaaa tcattgcaaa
    47221 aggtttttct cgtctaaaaa cacaaaacaa tcctcaagcg gtgcaacctt tgattattga
    47281 ataccaagta gaaacgattg agactaatca gtatcaccca gagctttcta ttttggcact
    47341 ttacttgcgt atcggttcat tggcatcacc gaaaaaaaca tatatcgtaa aaaacatcta
    47401 tgatttttta caagcttaca cagaaaaaga aacttacact gttaataaac agtaccagtt
    47461 tcgtctagat aaacgggcgt ttcaaaaaga agatcaaaaa atactcacgc atcttgcagg
    47521 aagtgctcag acgcatcaat tattaggtac gaacggctta caagtcaaag gaaaactaga
    47581 taaaagatat ctgcttctcc cagtagaaca agcccgcttc ctgttagagc agatgaatca
    47641 gactacacgt ttgcatctgc agataaatga acaaaacgat caaggaatac aattttccga
    47701 tcaaaacaaa cctttatcat tcaaagtgga aaaatttgga gaagattatt tgctgcaagc
    47761 attgaatgat tttgattttt tctttatgca ttatcagtgg ggagttatcc aagggacatt
    47821 ttattctctg acgaaatatc aacaaacggt ttatcaaact tggaaacaat tgattcgccg
    47881 attggagaaa ccagaagtag tgttcaagaa aaaagaatta ccgtctttgt ttaaagagat
    47941 tatccctctg ctgtcagaga tcggcgacgt agctattgcc cctgcagtag aagaggaagt
    48001 agcagatatc cccgtcgaat ttattttctt tttccgaaaa gcaaaaggaa aaatccaagc
    48061 tcgagtcgat ttcgtctatg gcgaagtcat ctattcgact gacccgaagc atgaggtctc
    48121 gacagaacag gcttttcagg tgttacgtga cacagtagaa gagaaacgag tacttgatct
    48181 attcagaatg tatcgctacc agaaaaatga aactggttat gaacgtccat tgccaagcgg
    48241 ggaagaacta tatgcctttt tccgcacaga actatctgtc ttccgtcagt ttggtgaagt
    48301 cagacttgga agaaaattgc gagagttgta tttagatgcg cataaacatc aaccgaaaat
    48361 cgaagtaacc gagaccggtt catggctcga tattcgattc gatgtgacag ggatagagga
    48421 gcaagaaatc gatcatgtcc tgcaaagctt gctgagaaat gatgcttttt atacattaga
    48481 aaatggtcag gttctttctt ttgattcaga agaatttcaa caaacaagcc aagtgttgca
    48541 acaattacgg gaatcgatac gtacagaaga aggaacgatc catgtaccta aaaatcaagg
    48601 attgatcatt caaaatcaat tggaaaaaag caatgctact tttagtgaat cgttccaaac
    48661 gatggtacaa gatctcattc atccagaaag ataccaggca cagcttccca aaggattgaa
    48721 tgcaacgatg agagattatc aaaagcaagg ttttcgatgg ttgaagatgt taggtcatta
    48781 tcagtttggc ggtattttag ccgatgaaat gggacttggg aaaacactac agacaattgc
    48841 ctttttactt tcggaaaaag aagaacggaa aagcttctct gctttgatcg tagcgcctgc
    48901 gagcttaatt tacaactggc aggcagaggt gagaaaattc gccccttctt taagtatcca
    48961 agtaatcaat gggaataaaa aagaacgtga agaattactg gctaaagaca cagatatccg
    49021 agtgacttct tatgccagct taagacagga tctagctaat tatcagtcgc aaaaaattga
    49081 ttatttgatt cttgatgagg cacaaatggt caaaaatagc agtacgaaaa ctgcacaagc
    49141 attgagagaa ttagctgtac ctcagcggtt tgcactgagt gggacaccaa tcgagaataa
    49201 tttagaagaa ttgtggtcat tgtttgcaac gattatgcca ggatttttcc cgactaaaac
    49261 aaaattcagg gaacttgccg cagaagaaat tgcgcaaatg attcgaccgt ttattttacg
    49321 gcgagataaa caaactgtgt tgaaagactt gccggaaaaa acagagatga atctatattc
    49381 tgcacttaca gaagaacaaa aaactgttta tttagcttac ttgcgtcaaa tgcgtgaaga
    49441 aatcacatca atggattctg aagcattcaa gaaaaaccgg atcgggatac ttgctggtct
    49501 gacaagactg cgacaaattt gctgcgatcc tcgtctgttt attgaagatt accaaggcgg
    49561 ttccggcaag ctcgaacaag taaaagatct actggtagct gccaaagaga acaaacgaag
    49621 agttttgctg ttttctcaat ttacagggat gctgactatc ttgcaagaag agttagccga
    49681 gctagggatc tcgacatttt atttaagagg cagtacgaaa ccacaagatc ggttaagtat
    49741 ggtagacgca tttaatgctg gcgaaaaaga tgtttttctg atttccttaa aggctggggg
    49801 aactggattg aatctcacgg gagcagatac ggtcatcttg tatgaccttt ggtggaatcc
    49861 tgccattgaa gaacaagctg ctggacgtgc acatcggatc ggacaaaaaa atgtggtaga
    49921 agtgtggcgt atgatctctg aaggaacgat tgaggaacgc atggattcac tccagcaaga
    49981 aaaacgcgaa ctgttccaaa aagtgatcca aggtaatgaa gaacagttga cgaaactgac
    50041 tgaagaagac atccgcatga ttttaagtgt aggagaaatc gaggagtaag agactcgaaa
    50101 aagcgttcag taatgggtaa gaaagccttt ctaaagaagc gattgtttat ttgatttaaa
    50161 aaatagcaag gaggctggga ttccatttta tcccagcttc cttgctatca tttttttatt
    50221 tctattttta aaggttatac aaagattgtc ttcattgtct ctaacagctg atcaaaagaa
    50281 gaaatctcga tatcgcaaat tggagtctgt tcaattttgc gttctcgatg gttgaaccaa
    50341 agcgcttgcc agttagcact ttttgcacct agaacatcat tgtcgtaatt atcgccaacg
    50401 tataaggtct cttctggaag catatgaaat tctttctcag ctagctgaaa aatctctttt
    50461 tctggttttt ggaaacctgt tgcttgagaa atgagcatat tgcgggaagg aatccaattg
    50521 ttcaattgaa gttgcttcag tttttttgtc tgatggtcag tcggcccatt tgtgataatt
    50581 cctaatggga cattcttttc cttcaagaaa tcgagggttt ttttgacctc ctcatgcaag
    50641 cagatattat caagttcttc ctcgtaaatt ttttgaaata agaggccatc ttcttccgta
    50701 atatgagggt agtctaaatc ttttagtgat tggctgatcc gatgtgctcg catatattct
    50761 agtgtccaat ctcctgccat tacttttggg aagttttcat cactatgatg gcggaaccgg
    50821 atatatagtg gatgcatatc tgcatctgaa acaagcggaa cgactctttt gaccgcgttt
    50881 cgaaatggct gctgctgatc atacatcgta tcatcaacat caaacacaac tgatttaatc
    50941 aaaattttac acgcctttcg gttttcattg attttttcac aaatccttga ctattgtaac
    51001 agaaaaaatt gtctaaaaaa gctgataaat aaaggttttt cttactatat ttctatttcc
    51061 ttgacagaat aaggttttta tatttcagtt tttttcacta aaaatcgtga aacacgtcaa
    51121 taagtttgat gaacaaatta atcattatgg tagaagtcga ttgatttatt ttaatgcaat
    51181 ctctgtggaa ttttcgaaag atttattttt ttaatacagt ctcaatataa ctattcaaac
    51241 ataaaataac gttatttgat ttatttgttt atagtaaatt ttatttagta atcattgctt
    51301 taaaatacta cgcttatata acagagaaaa taagcgttat aataaagaac agtattattt
    51361 aaaatgacag aaaacattac tattaaagaa ataaaaaaca taaaaaatct taagaaatcg
    51421 gtttcattta gcttttgaga taaaataata gtacagtttt atacgctgtt tcatatgttt
    51481 agaaaatttg aattagattt gaaaactcta atttttaaat gataaaaaaa gcgagaatgc
    51541 tggttttact gtatattaac cttagataac tttcattttt ttaaaaaaat tatgaaaaaa
    51601 aggtagctat ttaaaagttg attatgtata atagaaacag atgggagatt attatgatct
    51661 tctgttacaa aaaagtgatt cttatcacga aaaggagtgg tgcacgttgt taacattata
    51721 tacttcccca agttgtactt cctgcagaaa ggctcgtgcg tggttgcaag aacatcagat
    51781 tccatttgtt gaacgtaata ttttttcaga accattgaac agttctgaat taaaagcgat
    51841 tttacaaatg acagaagacg gaacagaaga aatcatctcg acacgttcca aagtattcca
    51901 aaaactgaac atggatttag atgatctgcc gttacaagaa ttattggaac ttgtgcaaaa
    51961 caatcctgga ttgcttcgcc gtccaatcat gattgatgat aaacgtctgc aagttggttt
    52021 taatgaagat gaaattcgtc ggttcctacc gcgcgatgta cgacaattag aattacgtca
    52081 agcacaattg atggctggat tatagaagga taatacgaaa cttggaaaag actattacaa
    52141 agatagtttt tccaagtttt tttgttttta caaaaagtat tcttgattag atgtataaaa
    52201 aggtagtaga agatgatgat tgcacgaaaa atagagaggg aaaattagct gaaaagtcgg
    52261 ctatatagta ttcgtctgcc aaaaatgtta tagttaaata agatagcttg aaaggttgaa
    52321 aaatccgctg gttttattaa attcgcttaa cagcgagatt ttttttcttt acatttaagc
    52381 tgatttcgct acaatgaatc tatattaaac attgtggcaa agtgaggtgt agcaaaatgg
    52441 aaatggaaca tattaatgaa aatacgatcc gtgtactaat tggtcatgaa gatttagagg
    52501 aacggggcgt ttcatttttg gatttacttg gcaatcacaa agaaatcgaa aatttctttt
    52561 atagtatttt ggaagaagtt gatatcgaag aggagttccg aggaagcgaa gctgtcactt
    52621 tccaagtttt gccaaaagga gacggattag aactgtttat cagtaaaaat cttccggatg
    52681 aagaaatttc tcaatcagaa gattctactg ttgattcaga agatgtgagt gactttcttc
    52741 gtcagcagat tattggcagc gatccggagg aagaattatc tggaagcgcc ctagaagaac
    52801 aggaaagaca agcaactttt gcttttgaag attttgaaca agtgatccaa cttgcaacag
    52861 atgtcgaact agagtctgct tggactgatt tgtatttatt cgaagagcgc tattacctag
    52921 tcgttcactt ctggatggaa aatctcaacc aagcggatgt agagaatcag attgctcgaa
    52981 ttttggagtt tgctgaaaag agcgatcgta caccagaaac attgaatgaa tatggacaat
    53041 gtctgatggc gcgtaatgct atcgagcgaa cgaaatttta ttttgtataa atcagcatta
    53101 tagtaagaga ctgtgacaaa aattgtgtcg cagtcttttt ttcgtataaa tgtgttcaaa
    53161 agcttgcatc tgcggtaaca agcccaacca aacgaaaaat attgggaact atttttgttt
    53221 agctgttctt attacttgat gcagaatagc tttttcacgc cctctttttt cgtgtaaata
    53281 aattcttaga cgtaaataag ttcagaggga gaaaaatgct gcttgcaatg aatgaatata
    53341 ataagtgctg ttttgctaaa catgcagaaa aaggaaagaa atattattgt cgtggctgtc
    53401 gaaaaagagt gattttgaaa aagggaaaga aaaaatgtgc tcattttgcc catcaaaaat
    53461 cagataactg ttcggtcttt agtgaaagag aatcaaaaga gcacttacag ttaaaggaat
    53521 gcttcatgga ttggctggga cagtcagtag aaccagtatt tttggaagcc tatttgccga
    53581 ggttgcggca aaggccggat attttgttgg cgaatctagc aatagaaatc caatgttcta
    53641 gactgtctca ccagagattt attgaaagaa cccaaaatta tttgaataac agttaccaag
    53701 tatggtggat acttggtaat tcttttcttg gacaatcgca attctcattg atagaaaaaa
    53761 gttgctgcta ctataataga aaaagaggag tgcattgctg gaaagctgat ctcaagaaac
    53821 aaaagcttta tttgtatcat catattacag agactgtatc agggcatatt tccttttgca
    53881 gtagctgctg gactttttct agtagggacc ttaaagaaat attcactagt aacgaaatta
    53941 aaatgaacca aatgaaaaaa atagaaaaag tgagtgaaga tgggaagaaa tggctgacac
    54001 gccaacttat tcacaagcaa aagaatacag tatccataca agaacaatgc tacttgcgtc
    54061 ataaacatct tttgcatcta tcatcgtgga tctatcaaaa aagtcggttt ttcttttatt
    54121 tacaagaaca ggtttttctt tatcgaatgt tgtacgaaga agccttggaa aaccagaaag
    54181 ttcctgattt caactcatgg ctttgccaaa taaaagaaca taaaagggaa tggctttttc
    54241 ctatgattga tgaagagatg gtctatcagc agttttttaa cgaatgcata cacttgtctt
    54301 ctttgaaaaa atagtaagta taaaaatagg ctaggaatcg gttttctatg taaaacaaga
    54361 tttagttatg ctaaaattga tggaagcaga cacatgaatg agtagaaaag ttagatgctg
    54421 tgacatgtgg atgttagact atgatctatt ttgatagata gagctaaggg aagaaaagga
    54481 gtggatttca tggaagtaaa gcagttgcca aaacgagaag aattgcctga aaatttaact
    54541 tgggacttga ccaagatctt ttcaagcgac caagagtttg atgagaaata tttggaatta
    54601 tcagaagagt taaaacaatc tgaaaaacac aaaggaacac ttgatcaagg cgcttctcaa
    54661 tttttaaatg cgattgaatt cgtattgagg gtttatcgcc aaactgaagt catttatgta
    54721 tatgcgcacc ttaaaaacga tcaagacact ggaaatacag attaccaagc gctttatgca
    54781 agagcaagca gtctgttttc gaaagttagt gaagccgttt cctggtttga accagaaata
    54841 ttgcaattgt cagatgacca gatttggcaa tatttcaaag aagaaccaaa attggaagtc
    54901 tatcgccatt atatccagca aatagtagat aatcgagccc atgtcttatc tgctgagcag
    54961 gaatctcttc ttgctggagc aggtgaaatc tttgatgctt caagtgatac atttgctgtt
    55021 ttgaataatg cagatctagt ttttccaacg attgaaggag aaaatggtga aatagtccaa
    55081 ttatctcatg gcgtgtatgg tcagttgcta gaaagcacgg atcgaagggt gcgcgaagca
    55141 gcatttaagg ggttgtacag tgtttacgaa caatttagaa atacatttgc ttctacttta
    55201 ggcacacata taaaaggaca taattttaaa gcgaaagtcc gtaattacag ctctgccaga
    55261 gaagcgtctt tgagcaataa tcatattcct gaaagtgtat acgatacttt ggtagacgtg
    55321 gtaaacaagc atttgccttt gttacatcga tacatggaat tacggaaacg tttattagaa
    55381 gtggaaaaac tgcacatgta tgatctttat acaccggtct taggggaagc tccaattacc
    55441 tttacgtacg aagaagcaaa agaaaaagct ttagaagcac tgaaaccaat gggtgaagaa
    55501 tacatggcca tcgtagaaaa agcattctct gaacgttgga tcgatgttgt cgaaaataaa
    55561 gggaaacgaa gcggtgctta ttcttcggga agctatgaca caaatccata tattttattg
    55621 aattggcatg atacgctgga tcagctattt acgcttgtcc acgaaatggg acatagtgtt
    55681 catagttatt tcactcgttc gaaccagcct tatgtgtacg gcgactactc catcttttta
    55741 gcagaaattg cttcgacaac gaatgaaaat atcctaacgg agtatttatt ggaaacagaa
    55801 aaagaccctc gtgtacgggc ttatgtactc aaccattact tggatgggtt taaaggaaca
    55861 gttttccgcc agacacaatt cgctgaattc gagcatttca tgcatacgga agatgaaaaa
    55921 ggtgtgccat tgactagtga atacctaagt gatagttatg gtaaattgaa tgcaaaatat
    55981 tatggtccag cagtcgaaga agacccagaa attaaattcg aatggtcacg gataccgcat
    56041 ttttattata attactatgt tttccaatac tcgactggct tttctgctgc ttcagcactg
    56101 gcgaagaaaa tactaaacca ggaaccagag gcactagaga actacttggc ttacctaaaa
    56161 gcaggtaaca gcgattatcc tgtggaagta atgaaaaaag caggagttga tatgacacag
    56221 gctgcatata ttgaagatgc aatgtcgatg tttgaacaac gtctaaatga attagaagaa
    56281 ttgatcgatc gtttataagg ctttctattt ttgattttct cgaatagaaa cgaaaaaaat
    56341 acgtcggctc tcttgtaagg gagtcgacgt attttttta
//
LOCUS       DANKEF010000014        56177 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000014 DANKEF010000000
VERSION     DANKEF010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 56177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.014"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(81..1637)
                     /gene="rny"
                     /locus_tag="PWZ48_000941"
     CDS             complement(81..1637)
                     /gene="rny"
                     /locus_tag="PWZ48_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HDL2260614.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(2048..3097)
                     /gene="recA"
                     /locus_tag="PWZ48_000942"
     CDS             complement(2048..3097)
                     /gene="recA"
                     /locus_tag="PWZ48_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDL2260615.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(3206..4447)
                     /locus_tag="PWZ48_000943"
     CDS             complement(3206..4447)
                     /locus_tag="PWZ48_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HDL2260616.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(4687..5265)
                     /gene="pgsA"
                     /locus_tag="PWZ48_000944"
     CDS             complement(4687..5265)
                     /gene="pgsA"
                     /locus_tag="PWZ48_000944"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDL2260617.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(5474..6397)
                     /locus_tag="PWZ48_000945"
     CDS             complement(5474..6397)
                     /locus_tag="PWZ48_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260618.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(7136..8422)
                     /locus_tag="PWZ48_000946"
     CDS             complement(7136..8422)
                     /locus_tag="PWZ48_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2260619.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(8415..9683)
                     /locus_tag="PWZ48_000947"
     CDS             complement(8415..9683)
                     /locus_tag="PWZ48_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDL2260620.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYPDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            9935..10396
                     /gene="mscL"
                     /locus_tag="PWZ48_000948"
     CDS             9935..10396
                     /gene="mscL"
                     /locus_tag="PWZ48_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDL2260621.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(10455..10901)
                     /locus_tag="PWZ48_000949"
     CDS             complement(10455..10901)
                     /locus_tag="PWZ48_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260622.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGQ
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(10994..11239)
                     /locus_tag="PWZ48_000950"
     CDS             complement(10994..11239)
                     /locus_tag="PWZ48_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HDL2260623.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(11354..11932)
                     /locus_tag="PWZ48_000951"
     CDS             complement(11354..11932)
                     /locus_tag="PWZ48_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2260624.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSIISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(12009..12539)
                     /locus_tag="PWZ48_000952"
     CDS             complement(12009..12539)
                     /locus_tag="PWZ48_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HDL2260625.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(12543..13403)
                     /gene="rlmB"
                     /locus_tag="PWZ48_000953"
     CDS             complement(12543..13403)
                     /gene="rlmB"
                     /locus_tag="PWZ48_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDL2260626.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(13396..13791)
                     /locus_tag="PWZ48_000954"
     CDS             complement(13396..13791)
                     /locus_tag="PWZ48_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HDL2260627.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(13788..15200)
                     /locus_tag="PWZ48_000955"
     CDS             complement(13788..15200)
                     /locus_tag="PWZ48_000955"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDL2260628.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(15197..15733)
                     /gene="cysE"
                     /locus_tag="PWZ48_000956"
     CDS             complement(15197..15733)
                     /gene="cysE"
                     /locus_tag="PWZ48_000956"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDL2260629.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(15819..16058)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(16055..17515)
                     /locus_tag="PWZ48_000957"
     CDS             complement(16055..17515)
                     /locus_tag="PWZ48_000957"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDL2260630.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(17685..18842)
                     /locus_tag="PWZ48_000958"
     CDS             complement(17685..18842)
                     /locus_tag="PWZ48_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HDL2260631.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(18876..20246)
                     /gene="radA"
                     /locus_tag="PWZ48_000959"
     CDS             complement(18876..20246)
                     /gene="radA"
                     /locus_tag="PWZ48_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDL2260632.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKIAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(20309..20836)
                     /locus_tag="PWZ48_000960"
     CDS             complement(20309..20836)
                     /locus_tag="PWZ48_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDL2260633.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            21066..21872
                     /gene="proB"
                     /locus_tag="PWZ48_000961"
     CDS             21066..21872
                     /gene="proB"
                     /locus_tag="PWZ48_000961"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDL2260634.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            21869..23116
                     /locus_tag="PWZ48_000962"
     CDS             21869..23116
                     /locus_tag="PWZ48_000962"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDL2260635.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVTGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            23192..23536
                     /locus_tag="PWZ48_000963"
     CDS             23192..23536
                     /locus_tag="PWZ48_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260636.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(23653..23979)
                     /locus_tag="PWZ48_000964"
     CDS             complement(23653..23979)
                     /locus_tag="PWZ48_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260637.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(24352..25553)
                     /locus_tag="PWZ48_000965"
                     /pseudo
     CDS             complement(24352..25553)
                     /locus_tag="PWZ48_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            25670..26185
                     /locus_tag="PWZ48_000966"
     CDS             25670..26185
                     /locus_tag="PWZ48_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2260638.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(26384..26830)
                     /locus_tag="PWZ48_000967"
     CDS             complement(26384..26830)
                     /locus_tag="PWZ48_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2260639.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(26877..28355)
                     /gene="msr(C)"
                     /locus_tag="PWZ48_000968"
     CDS             complement(26877..28355)
                     /gene="msr(C)"
                     /locus_tag="PWZ48_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2260640.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(28709..29338)
                     /locus_tag="PWZ48_000969"
     CDS             complement(28709..29338)
                     /locus_tag="PWZ48_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2260641.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            complement(29322..29786)
                     /locus_tag="PWZ48_000970"
     CDS             complement(29322..29786)
                     /locus_tag="PWZ48_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2260642.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(30273..31541)
                     /locus_tag="PWZ48_000971"
     CDS             complement(30273..31541)
                     /locus_tag="PWZ48_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2260643.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(31772..32509)
                     /locus_tag="PWZ48_000972"
     CDS             complement(31772..32509)
                     /locus_tag="PWZ48_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2260644.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(32576..33145)
                     /locus_tag="PWZ48_000973"
     CDS             complement(32576..33145)
                     /locus_tag="PWZ48_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HDL2260645.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            33273..34145
                     /locus_tag="PWZ48_000974"
     CDS             33273..34145
                     /locus_tag="PWZ48_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2260646.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKIFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            34487..35359
                     /locus_tag="PWZ48_000975"
     CDS             34487..35359
                     /locus_tag="PWZ48_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2260647.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            35356..37314
                     /locus_tag="PWZ48_000976"
     CDS             35356..37314
                     /locus_tag="PWZ48_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2260648.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAAARPAQTNTVVKTETFAGETIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVEEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(37565..38416)
                     /locus_tag="PWZ48_000977"
     CDS             complement(37565..38416)
                     /locus_tag="PWZ48_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2260649.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKELEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            38539..39006
                     /locus_tag="PWZ48_000978"
     CDS             38539..39006
                     /locus_tag="PWZ48_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HDL2260650.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(39269..39751)
                     /locus_tag="PWZ48_000979"
     CDS             complement(39269..39751)
                     /locus_tag="PWZ48_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2260651.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVSA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(39851..40714)
                     /locus_tag="PWZ48_000980"
     CDS             complement(39851..40714)
                     /locus_tag="PWZ48_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2260652.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGDRLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYGLVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            41312..42160
                     /locus_tag="PWZ48_000981"
     CDS             41312..42160
                     /locus_tag="PWZ48_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260653.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPHFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKLKKR"
     gene            complement(42331..42672)
                     /locus_tag="PWZ48_000982"
     CDS             complement(42331..42672)
                     /locus_tag="PWZ48_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
                     /protein_id="HDL2260654.1"
                     /translation="MVGLKRKELAGRRFTRIVSVGGGAKNLAWLQIQADIFDATVYCL
                     SMEEGPALGATMIAALGLGWFDTPESCCQNFIQYTDAAEPLLQNVEKYHKIYQQCRKI
                     YPATKEICHDL"
     gene            42758..43465
                     /locus_tag="PWZ48_000983"
     CDS             42758..43465
                     /locus_tag="PWZ48_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2260655.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLVYAGSAQFITVSMLASHSPLLSIVFSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVIVV
                     VTLIGCGLGVWIKHAFF"
     gene            43452..43775
                     /locus_tag="PWZ48_000984"
     CDS             43452..43775
                     /locus_tag="PWZ48_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2260656.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRVLPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLAGILSLS
                     LIRLL"
     gene            complement(43864..44901)
                     /locus_tag="PWZ48_000985"
     CDS             complement(43864..44901)
                     /locus_tag="PWZ48_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2260657.1"
                     /translation="MSEKELMKVVGFYEGLPIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGSGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHML
                     GKVVVSGPFNG"
     gene            complement(45348..46331)
                     /locus_tag="PWZ48_000986"
     CDS             complement(45348..46331)
                     /locus_tag="PWZ48_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HDL2260658.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKDPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILETFEKEHPTLFSEQ"
     gene            complement(46747..47517)
                     /locus_tag="PWZ48_000987"
     CDS             complement(46747..47517)
                     /locus_tag="PWZ48_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260659.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(47510..48445)
                     /locus_tag="PWZ48_000988"
     CDS             complement(47510..48445)
                     /locus_tag="PWZ48_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260660.1"
                     /translation="MANALEINNLKKIYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDKDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYDTKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(49039..50688)
                     /locus_tag="PWZ48_000989"
     CDS             complement(49039..50688)
                     /locus_tag="PWZ48_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2260661.1"
                     /translation="MGQREFIVLIIIAVVILLVVLDIFSRLKVKETVRSNWGKIPYQP
                     RFDKEESLKDAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDYQLEKLIAFYQENPQLRERIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGVGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKMTTPLQKELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAVLNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLNYPKSIVEQAQKESLLFDEQRQWYPFD"
     gene            complement(50883..51779)
                     /locus_tag="PWZ48_000990"
     CDS             complement(50883..51779)
                     /locus_tag="PWZ48_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2260662.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWVDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDSFIRLTVANPIKPGAQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(51772..52452)
                     /locus_tag="PWZ48_000991"
     CDS             complement(51772..52452)
                     /locus_tag="PWZ48_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2260663.1"
                     /translation="MFLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(52453..53235)
                     /locus_tag="PWZ48_000992"
     CDS             complement(52453..53235)
                     /locus_tag="PWZ48_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260664.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMIAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LILATLSVPVALTFITRSTSVSLLLSFLYIMGTAFVPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(53228..54145)
                     /locus_tag="PWZ48_000993"
     CDS             complement(53228..54145)
                     /locus_tag="PWZ48_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260665.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(54148..54432)
                     /locus_tag="PWZ48_000994"
     CDS             complement(54148..54432)
                     /locus_tag="PWZ48_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260666.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWQTTQPRLRDSLIFLEIQLENLHFLYTFLAAFFIPVNYTSLINKKEG"
     gene            complement(55133..55753)
                     /locus_tag="PWZ48_000995"
     CDS             complement(55133..55753)
                     /locus_tag="PWZ48_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260667.1"
                     /translation="MNINRVIKLIGAKNINKVRSEFIFPEDFFNWFFYQFSECKRLLG
                     ENYYLKGIGGFMGNVADEQNIIKGISEQTSELTVTKAFISNGESLKNVTARIRKENEV
                     DIVFGIDHECNSVIYVTQSANLILFENGCKDDFMVVYLYDILIPKLRSLYMSSESIFV
                     SEQKGSFSKKIGIEVIESIMCHNHITLEELEILIGQKNNNTKIQVS"
     gene            complement(55834..>56177)
                     /locus_tag="PWZ48_000996"
     CDS             complement(55834..>56177)
                     /locus_tag="PWZ48_000996"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260668.1"
                     /translation="NIKTSTKLICKLFKLDKISNIDDVKSKILTRKVSEPVKLTAQDK
                     KNGIVLYGDDAKYNYNWDTSSEAEVLSIPTTDTSGEKVTFKINFITAISKLEYGKQKK
                     RDNNDQYLPKK"
BASE COUNT        16071 a        11960 c         9965 g        18181 t
ORIGIN      
        1 aatgatagca atgttttttc gtgccttaca aaaaaagaag agctgtggct tttgccgcgg
       61 ctcttctttt gtatttttta ttatttagca taatcagttg cacgagtttc ccgaataacg
      121 attactttga tgtgtccagg ataatccagt tcttcttcga ttcgtttacg aatatcacgt
      181 actaaacgaa cagcgtctaa atccgagatt tcttctggct tgaccattac ccgaacttcg
      241 cgtccagctt gaactgcgta acttgattct acaccggcga agctgttcga aatattttcc
      301 agacttctta aacgacggat gtagttttct aatgattcac ttcgcgcacc aggtcttgct
      361 gctgagagcg catctgctgc agctacaagg acagagataa cagaagtagc ttccacatct
      421 ccatgatgag aagcgatcgc gttgattact actgggtttt ctttgtactt ggcagctaat
      481 tccgcaccga tctcaacgtg agatccttca atctcatggt caagtgcttt cccaatatca
      541 tggagcaacc cggcacgttt tgctaattgg atatcttcgc caagctctgc tgctaagata
      601 ccagctaatt ttgcaacctc gaccgaatgc ttcaagacgt tttgtccata actagttctg
      661 aatcgcagac gtccgatgat cttgattaga tctggatgaa gtgtatgagc accgacttca
      721 aacgctgcct cttccccgta ttcgcgaata cgttcatcca tttctttacg agatttctcg
      781 accatttctt cgatacgcgc tggatggatt cgtccatctt gtatcagttt ttccaaggtc
      841 atccgtgcga tttctcttcg gattggatcg aatccggaaa gaacaactgc ttccggtgta
      901 tcatcgatga ttaaatcgat accagtcagc gtctcaagcg ttcgaatatt acgaccttct
      961 cgtccaatga tacgaccttt catttcatca ttcggaaggg taacgactga aacagtcgtt
     1021 tctgaaactt gatctgctgc gcagcgttga atcgctaatg ataataagtt tttcgctttg
     1081 cgatctgctt cttctttggc acgctgttca gattctttta ccattagagt taactcatga
     1141 ttcagctctt cttcagtcga tttcatgatg acatctcgtg cgtcctcttt tgataaacca
     1201 gcaatttttt ctagttcttt ttgttgatct tcaatcattt tttcaacgtc tttttctcgc
     1261 tcttcaatta attgctgttt tgaactaagt gcttcttctt tactttcaag tgagttctcg
     1321 cgtttttcta acgagtcatc tttacgattc agcgtttgtt cgcgttgaat caaacgattc
     1381 tcttgagatt ttaattctaa cttactttct ctcagctcac tttcaacttc agaacggtat
     1441 ttctgatttt cttccttagc ttctaacaat gcttcttttt ttagagtttc tgcttctttt
     1501 tttgcactat cgatgattcc agcagctgat gattgtgcac cagctatttc tttctcgtga
     1561 cgtgactttg cgaccataaa ccctaaacca agaccgacaa ttaaaccgat gatagcgagg
     1621 agaatgttga ataccataaa atccacctcc atactatctt tttcattttt atcatctata
     1681 cagcttctat gataaattat tctaaactcg tcaatatgca tcttcgcata tatgtgtaca
     1741 aaaaacatgc acaactttat tctaatgttt gtaccccgta gtgtcaactt taaataaaca
     1801 aagaaacggc tttctactta ttcattaaac gatggttttt aaaataaatg acactagttt
     1861 catataggta aacctttaaa ttgagaaact tttctattaa attattcttt gtagatgaac
     1921 agatattttt attttatgta cccactcgta taattaaata actcacgatt tctttattca
     1981 ataaaaaaac ggcaggtcaa ggagagtacc tactcttcag accagccgtt tttgtttatc
     2041 ataattttta ttcttctact aaaggcaatt cttcttgtcc tttttcatct tctgggacag
     2101 cgacttcttc accaatacca taagctgctc taactagagc agaaacttct gccatcattt
     2161 caggatgatt agccatatag attttagcgt tttcccgtcc ttgaccaatc cgatcttctt
     2221 tataagaata ccaagcaccg cttttatcaa caatgtcttt ttctactgcc atgtctagaa
     2281 gttctccttc ttgtgagatc cctaaaccat acatgacatc tacttcagcg attttgaaag
     2341 gaggtgctac tttgtttttt acgactttga tctttgtacg gttaccaaca atatctgtac
     2401 cttgtttcaa ttgttctgca cgacgtactt ccaaacggat cgttgcgtag aatttcaatg
     2461 cgcgtcctcc aggagtgatt tctggattcc caaacatcac gcctactttt tcacggattt
     2521 ggttgatgaa aatagcgatt gtttttgttt tgttgatcga accagagagt ttacgcaatg
     2581 cttgagacat caaacgtgct tgtaacccga catgtgagtc acccatttcg ccgtcgattt
     2641 ctgctcgtgg aactaaagca gcaactgaat cgactactac gatatctaca gccccacttg
     2701 atactaaagc atcagcgatc tctagacctt gttctcctgt gtcaggctgt gaaagaagta
     2761 gttcatcgat attcacacct aatttttgtg catattgcgg atctaacgca tgctcagcat
     2821 caatgaaagc ggccgttccg ccattttttt gtacttctgc aatagcgtgt agtgcaactg
     2881 ttgttttacc tgaactttca ggtccatata cttcgatgat acgtccacgg gggtaaccgc
     2941 cgacacctaa agcgacatct aacgcaagag agccacttgg aattgtagag atttgttggt
     3001 cgattttttc gcccaatttc ataatagagc ctttaccata gctcttttca atttttttca
     3061 gtgcagcatc taaggctgct ttacgatcat ctgccaatac gataatcctc cttatattta
     3121 caaatcagag caacatttca ttatccttat catatctttt ttagtgaaaa aaagcaagaa
     3181 aaaaccgaac aaatattcgc atttatcatt ttcttaaaag ctgttttctc aaaagatcaa
     3241 atccttgcat aaccgcatta tatctgatag cagcacgatc tctggcaaag tgatattttt
     3301 ccgaaaccac gccgtcttta gatgaaatag cgatccatac tgttcctgca gattgacctt
     3361 ctaacgtgtc cggtccagcc actccggtga aagctacact gtaatctgtt cttgctatct
     3421 cacgtgcttg aatggccatc tgctccacac attctttgct gactgttcca tattctgcta
     3481 atagctccgg atcaatctcc agaaaaccag cttttgtttg gagcgagtag gtaacgaatc
     3541 cgcctggaaa tacttcagat acaccagcaa tgtcgcctaa tgcggcttgg aaggcgccgg
     3601 ctgtcaaact ttccgctgct gttacagtct gcttgttttc tttcagtagt tccactacga
     3661 cttttgctag cgaattatca tcgccgtatc cataaaaata ttcccctact cgttcttgga
     3721 ttttttcttc taatgctaaa agagcttgat tccctgcctg aacatcattt gttttcactg
     3781 tcagtcgtaa tgtcacttca tttggtttgg catatggtgc gatcgttgga ttgatctgtg
     3841 tctcaatcag atcttttagc tcagtgacca gtcgcgattc accaatccca taaaagcgca
     3901 aaactttaga aattagtttt tcttcagaag gaaaacgttc ctctagtaaa ggacgtactt
     3961 gttccacaaa cattggtttc aactcattag gcggaccggg aagaaggata tacgcatgtg
     4021 tatctgtttg ataaaaagtc cctaaagcaa gccctgtacg atttggtaga ggaaccccgc
     4081 cttcgataat ctgggactgt tgcagattgt ttttggtcat ttttcgatgg gttgtttcaa
     4141 aatatgccaa taattttttg tatccttccg tattttgtat gagagactta ccgaggtgag
     4201 ccgcagttgc ttcttttgtc aggtcatctt ctgtcggtcc taatccgcca cataagagta
     4261 tgagttcact tcttgtttca gctagagcaa tcaactcttc taaacgctgc tgattatctc
     4321 ctacaactgt ttgataataa acttcgatac ctagatcagc tagctgttct gacaagaaag
     4381 tggcattcgt attcacgact tgtcctagta aaagttctgt tccgacagct attatttctg
     4441 ctttcattac ttcccgctcc cttatatagt atacgctgta tttttatatt tttttattta
     4501 aaaagagggc aaggagctgt gacaaaaatc ttgtcacagc tccttgttcc aaataaacag
     4561 tgttctcctg ccaaaacctc ggtcataagt cacaatatac ataaaacatg aaaagctgtt
     4621 ttttgaaatt atcccttgtg actcagagtt agacggcagg ctcacaacct ctccactaaa
     4681 cgatacttac attgatcctt tgaacacacc gctgtttttg atgaagtaat ccacacctga
     4741 atagatagta aagatcagac aagcataaag catgatttga tctactggaa taccgatcag
     4801 gctgaatggg atattattga taaacagcaa gataatagca accatttgtg tagccgtctt
     4861 gacttttccc ggccaagctg cagccatgac ttctccatgt tcgacaagta gtaaacgcaa
     4921 gcctgtgacg gctaattcac gacagacaat gatcgctact acccaaccag gggcctttcc
     4981 ttggtcaacc aacatgataa aagcagtcat aactagcatt ttatctgcca acgggtccgc
     5041 aaacttgcca aaattagtga caaggcctcg cgcacgggca atttttccat ctaaccagtc
     5101 tgtgatacta gctaaagcaa aaataactgc acctactagc tgtgtgacag ccagtgtggt
     5161 gtctcctacg ttaaattgtc cccaatctaa aggtaaacct acaaccaaaa tgaaaatcgg
     5221 gatcatacaa attctaagta cagttaattt atttggtaaa ttcacactat cgactccttt
     5281 cttccctatt ctacgtgact atcagcgaag tttccaccga tattatttac ttttccaaat
     5341 gaatttattc aaaggagtat ttttattgaa attgatcttt ttgaattaac gaagaaggac
     5401 cgcgacacag atttgtcacg atccttcttt tttataccta caagtaaaca gatgcgtaaa
     5461 ctaatttata cgatcattct gcgtattgaa gagtaaggtt cacatttttt ggactctgat
     5521 tgttttcccc tggattaact ggtacggatt ctccattcgc tttgatcgtt gcattgcttg
     5581 ctgcaccaat cgtgatggtc gcatttgttg ctgtttccgg taacacggta ctctgtgttt
     5641 catttgctgc cagtgtccct tgataaacat aggcattgtc tactagcaca cctaaccata
     5701 cacgattgac agcagtaaat tcaagggtta caggcttggt agcgtctgtg atatttactg
     5761 cgatggcact gccagaatct tctcctgtag ctattgccat tttcttttct tcacttgatt
     5821 ttgttgttga ttctactgta gaagactctt tggtctgcgt gctttgctct gaggaagagg
     5881 actcctcgac tgatccttct actttaagcg aagaagctgt gtctccgatt attggatcag
     5941 cttgacgatc ttgccaagtc atatagccca cgacaacgac aatcgccaaa gcaaccaatc
     6001 ctaataaaat gacaggaaga cttgtccaaa agcgacttgg atgcgattct tcctgatgca
     6061 ttgcttttct agaagtactt actgtttcag gctgcggacg ttcagggacc gatcctgtca
     6121 atttgctttt cccatcaaaa acatcgacta aatggtctcc atcttctcct acagcttcag
     6181 cgtattgccg aataaatgcc cgcacgtaaa atttaccagg caattggtca aaatcatcat
     6241 tgtcgattgc ttccaaataa cgtttttgaa tttttgtaat ctgctgtaat tcatctaaag
     6301 atattttttt attcaggcgt gcttgccgta atttttctcc gatggtttca aaagccactc
     6361 gttcatcttc tccagtctgt ttgattctcc gaagcatctg ctgttgcaac gaaagacgtt
     6421 atgtttgggt gctgtgacaa aagtcatgtc acagctcctt ttttccgaat aaacattgtt
     6481 ctcctgccaa ctcctcggtc acaagtcaca atatccaata aatataagaa gctgtttttt
     6541 gaacttgttc cttgtgactc ggagttaaac ggcaggctca cgacctcttt cttaaacggt
     6601 gttcaatcag ctgttcctcg gtattagctc aaaaaccggc aaaatataag gagatatttt
     6661 tccgtatgtt tctctaatac tcaggtcaaa acgctgattt cacaacctct tccttaaacg
     6721 gtgttcaaaa gcttgcatct gcggtaataa gtccaaccaa gttaaaaaca ttaagaagct
     6781 gtttttactt ggctgttctt attacttgat gcagggcagc tttttcacaa cctcttggta
     6841 cacggtgttc cagaagtagc ttcttcggca ataagccgaa atatcacaaa aatttgagaa
     6901 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctcttt
     6961 gattacttcg tttgatttta ttttaatata agttgattaa aagagagcat gatttgtttt
     7021 attttctcac taatttttcc aacacaacta agaataccat aaaagctgat tttggaggag
     7081 gttcctacta caagtctaaa acgaaattta agacttttcc tcataaaata ctggtttatt
     7141 ttggtctcat gtaaaactcg ctgaaggcct cttcacttat aaatgcttcc ccagctgcca
     7201 gtacgtcttt catttggatc gaatcgatga tttcaggaag atcaaataaa gttcgatcgc
     7261 caaataaact ttgcgtaaat tgattggcaa tatattcgat cgaatttaat gattggaaat
     7321 attgtccaag cattttcttc ttcagcaaat cgagatttgt ttctgacact tccggatcat
     7381 cagcgaatcc aagaatgatt tctttgactt tttctgctgc tttctctggt tcgtccgtat
     7441 ctccgctgaa gtccgcaaag tgaaattcac gatccaagct gaattcaaaa ccaaagctgt
     7501 catcgataat tccttgattg tacatcgcta gatagtttcg agaagtattg cctaaaatca
     7561 tttggaacaa cagattgatt gctgttttat agcgtaacag ttctgttcct tcttgcggca
     7621 atgtgtctaa tcctttaata ccaagtacaa atttatctcg tgtaatggct gcttccaatg
     7681 cactttgttt gatgatttct tttgtgtttt cagggaaata acgtacaatt tcttgctttg
     7741 gagggaaatt ttttgcttct tggttttccc gaataagttt catcaatttc tcaggctcca
     7801 tcttaccaac tacaaataaa accatattgc ttggctgata aaacgtccga tagcacgtat
     7861 acaagtcttg agctgtgatt ttgtcgatac tttcaaccgt gcccgcaata tcgatatgca
     7921 atgggtgtgt aggatacaag ttatttagta tgccaaaaaa catccgccag ttagggtcgt
     7981 cttcatacat ttggatttcc tgaccgatga ttcctttttc cttgtttaca gtttcttctg
     8041 tgaagtaagg tgcttgtaca aaatcgatca gagtcgtcag attcttttct acctgatccg
     8101 tcgtagaaaa cagatagctg gttttcgtaa aacttgtgaa agcattggcg gaagctcctt
     8161 gtttcccaaa taattgaaag acatcaccat cttctttttc aaacaactta tgttctaaaa
     8221 aatgggcaat tccatctgga actttcacct tttctttttc accataggga ataaattcgt
     8281 tatcgatcga accataattc gtactgaaca aaccatatgt cttgtgatag tcatttttag
     8341 gcaacaaata cacggtcaaa ccgtttggca gtacttcatg ataaagtgtt tcattgattt
     8401 gctcatattc tgttttattc atttgcattt cctcctgcta aaaagaagat tgcctgcaat
     8461 tcgatttgct cagctacttg ttgtatctct ttgatcgtta cgctctccat acgtttcaac
     8521 cattcttcat cagataattt tgtttccggc agccagctgt ccaaaaaaga agcttcgatt
     8581 gccgcttgcg gagagtctag agaaagcaga tattggtttc tcagcattgc ttttgtctga
     8641 gacagttcca actctgttat ttcaccattt cgcaaacttt ctaattgctc gtgaatcaga
     8701 tgaagtactt gatttcggtt cttttcatcg ataccggttt ggacactcat gaatcctcgg
     8761 aacgtatcca cgctactaga ggcataataa gctagacttt ctttctctct gacattcatg
     8821 aaaagtttag agtgaggaaa accgccaaat aaaccgttaa agaccattag cgcaaaacgt
     8881 tctggttcat cgtaatacac atttgtctga taagctaggt tcagctttgc ttgaacgatt
     8941 ggctcacgta cttgtcgttc cttcacgata ttcacttgag gttgggtata aaacagatct
     9001 ggataaactg tttcccgcga ctcaaatggt aattgcccaa ctgcttcagc tgctttgtcc
     9061 ggatcaacat cgccgacaac aaaaatgtcg atttgatcat ttgccatcat ttcttgatag
     9121 taggacgcta gcgaactagc agtgatcttg tccaaatctt cgactacgcc caaccctggg
     9181 atcttttgtt ccgggctgtt ttcaaaataa agttcttgca aggcatagga agcaaaagtt
     9241 tgtttatctt ccttcaaact ttccaaatat agacgcagat tgttcttttc caagtcaaac
     9301 gtcaactgat caaactgatg atttttaata tttggataaa ataaaacctc ttttaaaaaa
     9361 tctaccgctt gagaaaaaag gtttggatca ttgacatatt caccattcac aaaactgatc
     9421 cctgcgttta gccaatgtaa atttcctttt tttctaactg ataatccaaa gcttgcgcca
     9481 taaagctctg caagttgcgc acttaacttt gtctgatccg gataattcaa actatttgtt
     9541 tccaacatgc tgctgagaag tgaacgtttc gtgatgactt cttgttgcag acgcgcagta
     9601 aaacggataa aaatccggat cgttttgtat tgttttgttg gtaagacatg aagattcacg
     9661 ccttctgcca attgatacac catatttata tgctcctttt cacagttgat tgcccgctta
     9721 ctcttttaat gggtcaacct gcctttttta cttattcctc tgcttgaaat ttataagttc
     9781 aggcaattcg ccgttttttc taaatgatgt tacactttat agtatagcat agatgttttt
     9841 tatcaaaaga aacagacggc taaaagcgta tattgttttt ttatttttta tttttcgata
     9901 tactaaagac aaataaaaga agggagggtc attcatgatc aaagaattca aagagttcat
     9961 catgcgtgga gatgtcttgg atcttgctgt cggcgttgtt attggtagtg cgtttacaag
    10021 catcgtaaac caagtggtgc aaggcttgat cacaccacta ataggttggt ttgtcgcaat
    10081 gatcacaggt acttctgatt tagaaggagc actttcaatt cttgactggt cgccgacaaa
    10141 aggcgtcaca tttgcgtttg gtaacgttat tagtgctatc attacatttc tgatcactgg
    10201 ttttgtctta ttcttagttg tcaaagcggc aaaccgtgcg aagaatttac gctccaaaga
    10261 agagcaagtg gaagtggaag aaactcctac tgcagaagat tatcttagtg acatacgcga
    10321 tcttcttgcc aaacaagtgg cagaagacaa tacacatcca gtagagcaaa caaatctttc
    10381 cgatacttct aaataataaa gaatctaaaa aaaagaaagc cgattttcag tgtcggcttt
    10441 ctttttttag attcttaact tgctacttgt tcctttttca tgtcgatatg tggaatatca
    10501 tcttccaaat acacatctga gattgccttg aatccaaacg actcataaaa agcttgtagg
    10561 tattcttgtg cgccgatcac gattggtttt tctggaaaat ttttctggat catgttaatc
    10621 gtttcttgaa caatctttct tcctaaacca tctttacggt tgtcctgatg aacaaggaca
    10681 cgtccaaaat ggatactctc cgcttcttca tatactcgcg tgtaggcaag gatcttccca
    10741 tcagtatctt gaaaaaacgt atgccaagct tgtccatcaa tgtcatcaac ttcttgataa
    10801 ggacactgtt gttccacaac aaaaactgcg attcgttctt tgacgatcgc gtaaaactct
    10861 gctgttgaca attcttcaaa tttctttacc tgatattcca ctaagcaaac gccccctgct
    10921 tttttattct tagcataccg attcgcacac aaaaaagcaa acgaacctta cgaatctcag
    10981 gctcgtttgc tttttaaact gcttcagtta aaaattcgat ctcaactgtc cgagtaagta
    11041 catctgaata gctgtaagat actcgttcaa aagaattttc ttcaggatcc aaatcaacaa
    11101 cgaatactga aggataggtt tctgttaaaa cgccgcggcg ttctgtttga cgctttcttc
    11161 ctgtctgagc aaccaacatg attgggctgc cgatacgacc ttctaaatct ttcttgattg
    11221 aagctaaagt tgttggcata cagttcacct ctactatcaa ctataccaca tatttttaaa
    11281 attttcaagt ttaccataaa aaaaaaagaa atgcaataaa aataaataag aagctcatta
    11341 agatttttct aaattattcc tcattataaa atttcaagtg tttttttatt ttgagtttta
    11401 ctctcccgta agcgctcatt acttgcttgt gatccattgc taaacattca gccatgtcta
    11461 caaaatcaac gccttgcata taataatgaa aaattttagc ttctattttt gaaaactgga
    11521 tttcgcaatt ttcgattgct tcttttaata aaagatgctg aatcgctaaa ggttcttgca
    11581 atgtgatctc tgctaaaaag tctggtcctt ctgtctctat tttttggtcg attgagactt
    11641 catctttttg atcacgacgc ttcagcgcgt tctgcttcct caacagagaa tagatttgat
    11701 tgcttataat gcttttataa taacttccaa agctaacacc tttgcttttt tcgtaagttc
    11761 tcaatgcctg atataaagcg atccttgctt cttgttccca atcctcagaa acataatttc
    11821 ttaaataata ggtgtttttc aaacgtagta aaagtggaaa atatttttga tacagggcag
    11881 caaatgcttc ttcgtcacca ttacgtgcag cgcgcaataa ctcctcagac ataaaataat
    11941 tcctccataa attcgtttat ggaggaatct tatcatcaat gattcatcga actgattaga
    12001 acgatatgtt atttattttt tctcattaaa tcgtccaatt tttgggacaa tttttttaac
    12061 tgctcttcat tccatggaga attacgtcgg aaatcaataa aatgcatatc tttattttct
    12121 tgggctattg ttttttccgt tttttttatc gtcttatata attcataagc agaagttcgt
    12181 aatgctcctt gggaaaagat catccactgt tctgctaaat cgcttgtcgc cactgtcact
    12241 tgtgtcaaac ggtcattcag ctgaccagcg attctctcga tataactgtc agccgtttcg
    12301 tcttctttcg taaaaacaac atccaactgg tatttcttat aattttgttg gatgcccgga
    12361 actagttggg catcgaagac aacgatgact tccattcctt catatttcgc ataatttgat
    12421 aaacgatgta aaagagcttc tcttgcatcc gccattttgt cctgcttttt aagctggaca
    12481 agttccggcc acgaaccaat catgttgtag ccgtctacaa tcaacagctg ttttttcatg
    12541 gtctactccc cctgacgttg ggaaaaagcc tgatacataa gcaaaccagc agcaacactg
    12601 gcatttaagc tctggacgtg cccggtcatc gggatcgtca gcaattcatc catttctttt
    12661 ttcaatcctg gactcattcc tttgccttca ttaccaatga ctaaagcaat tgatcctttg
    12721 gcattccatt tttggaagtt tgtgccggtc atatctgtgc caaataccca aaatcctgtt
    12781 tctttgagct gttgcacagc ttgtaccaaa ttcgtcacgc gtgcaacagg gacatgttcg
    12841 actgccccag tggaagtctt tgttacgata ggtgtgattc caactgctcg gtgttttggt
    12901 atgatcacac catcgacgcc acttgcgtct gctgtccgta aaatcgaccc aaaattgtgt
    12961 ggatcttcga tgctatccaa aatcaaaaag aaggggtctt cttttttctc ttttgttcgt
    13021 tcaatcagtt ggtcaaggga taaatattca taaggtgtaa tcgcaagaac gactccttga
    13081 tgcacgccgt gatcgctcaa agtttctaat ttttgtttag gaacccattt gactggcaca
    13141 gcttgttcac gtgcgatttc tttcagttct tctacttttt ctcctcgtga atcttcttga
    13201 agaaagagtt tattccctcg cccttgttgt agcgcttcta ctgcggcatg gtgcccaaag
    13261 acgaaattat cttctaatgc ttcttcattt tgatcagaaa ctgactgatt tcgtccagtt
    13321 tgctttttat ttgaatatct attttgtttt ttgtcttcaa atccacgttt cgattgatgt
    13381 ttgtttgttc tttctttacc catttttttc accaactttc tgaatacacc agcggatcaa
    13441 ttcttccatc cgctcggttt gttttgtcaa atgcagatag cccatgagcg cttcgaaccc
    13501 tgttgcgacc cgatacgtcg taacatcagc attttttgcg ctcgtatggc ttttagcatt
    13561 tcttcctctg cgataataaa gttcttcttc ttctgtcaaa agatgttctt ccagcatttg
    13621 ctgaatcaaa aaggcttgtg cattcgcaga aacataattc gttgccgcac gatgcagctt
    13681 attgggtttt gtttctcctt gatcaatcag gtggtcacga acatagattt cgtaaatcgc
    13741 atctccaaca taagccaagg ctaaaccgtt caattgtgtg taatctctca tgcttctctt
    13801 ctccatcggg ttccttgcgg tgtatcttct aataagatcc ctttttcttt caacaaatca
    13861 cgaatttcgt cgcttcggac aaaatcacga ttgcttcttg cttgatttcg ttcttcgatc
    13921 aattgttcaa tctcttcatc taataactct tctgctgtaa ataaaatacc gaagatcgaa
    13981 agccattgcg agaataaagc ttctccttgt gccaagacag cggcagaaac tttcggctgg
    14041 tctagataag tgttcagcca cttagcaagc tcatagacca ctgtgatccc atttgctgca
    14101 ttaaaatcat cgtccatttc ttcgatgaat cgctgttcta aattttctaa caaagcgatt
    14161 ttatccgcat catctgccaa attctctgag gcagtttctt ggcggaattt caaattgtca
    14221 aacgtattct tcagtcgttg taaattggtg gctgcttctt tcatcgtcgt ttcactgtag
    14281 cggatcggac gacggtattg tgtggtcgcc atgaagaaac gcaagacttg tggatcaacg
    14341 tttttgatca tttcatgtac agtgatgaaa ttccctagag atttactcat tttttcatcg
    14401 tcttcgccaa tcgttacata gccattgtgc atccagtaat tagcgaattt tttccctgtt
    14461 ttcgcttcac tttgtgcgat ttcattttca tgatgaggaa attctaaatc ttgcccgcca
    14521 ccatggatat cgatcgtttc tcctaaatgt tttgttgcca taacggaaca ttcaatatgc
    14581 caaccgggac gtcctttgcc ccatggtgaa tcccaagaaa tctcgccagg ttttgcttgt
    14641 ttccataaag cgaaatctaa tgggtcttct ttttgctgct gttcttcccc tgtacgctga
    14701 cttgctccga cttccagttc atcgatggat tggtcactta atttcccata attttcaaat
    14761 ttacgggtac ggtaatacac atcacctgaa gattcataag catagccttt ttcaaccaac
    14821 acttgaataa atgctaaaat atctggcata tgatccatga ctcttggatg cagtgtagca
    14881 ggctcgacat tcaacgcttt cgtatcttct tcaaatgctc ggatgaaacg atcagcaact
    14941 tctggtgctg taatacctaa ttcgtttgct gtacggatga ttttatcgtc tacatccgtg
    15001 aaattcgata cgaaatcgac tttatagcct cgatattcaa aatagcgtcg aattgtatcg
    15061 aaagcaatgg tgctacgagc atttccgata tggatataat tatacacagt cggtccgcag
    15121 acatacatac cgacatgtcc cttttgtata gggatgaatt cttctttttt tctggtcaat
    15181 gtattgtaga tttttatcat tctgatctcc tcactttttc tcctttgatt cgaacgactt
    15241 ttgctggtac tcctaccgct gtagcgtcgt ctggaacatc tttcaacaca acggcacctg
    15301 caccgatttt ggcgtttttt ccgatcgtta ccggtcctaa tatctgcgca ttcgcagaga
    15361 gcatggctcc ttgtttaaca gttgggtgtc ttttacccgt ttcttttcct gtgccgccta
    15421 atgttacgcc atggaataaa atcacatcgt cctcgatttc tgcggtttcg ccaatgacga
    15481 ttcccattcc atggtcgatg aacacgccag ttccaatcgt tgctcctgga tggatctcaa
    15541 caccagttag gaaacgccag aattgagcat tgatcttcgc caataaaaag agccggtgct
    15601 gatataaaaa atgagagaaa cgatgccaaa acaaggcatg aaaaccaggg tatgtcaaaa
    15661 cgatctcagc tgtcgaacgt gcagccggat cgtttctctt tgctgcatca atcgctcggg
    15721 taaaccaact catgactttc cactcctttc gtaagaattt tatgtaggaa atcatcttct
    15781 tataaagaaa agaagcgcct tttaaataag tttccttatc aaaagacgct ttccgcgtgg
    15841 ttccactttt cttcataatc aatgattatc tctgacagct aacgcctgtt atacgttctt
    15901 ggctactttg tttcctataa aacagttttt cctgttttct gtgtttcgcc aagaccactc
    15961 ccagaggcat ttcggcaacc attcattgat accttgcacc acccggtatc tctcttgtaa
    16021 tgatcagctg cttacttttt tctgttcatc gtgtttagtt cagtacttga tttaaatgtg
    16081 ctaatgattt ttcacgacca agcaattcga tcgtttcagg caattctgga ccatgcattt
    16141 gtcctgatac agctacacgg attggcatga aaagattttt tccttttacg ccagtttctt
    16201 tttgaacagc tttgatggca gctttgacag ttggagcatc tactacttcc atttgttcta
    16261 gctgtgcttt gaatgcgtgg agaacagtag gaactgtttc accagcaagt acttcttttg
    16321 ctgcttcatt caatacagga tgttcattga aaaatagcgt agacagatca acgatttctg
    16381 cagcatagct catttgaggt tgatacaggc taacgatctt cttcacccat tcgattgtct
    16441 tttcatctgg gttttctgct acacgtccat cagcgattag ataaggcaga cacatgtcag
    16501 taaatgcatc tagatccatt gctttgatgt aatggttgtt tacccattgc agttttttcg
    16561 catcgaaagc tgcgggtgat ttacttaggc gttttggatc gaacattttg atcaattctt
    16621 ggtgttcgaa gatttcgtct tcgcccacag gtgaccagcc caataatgca ataaagttga
    16681 acattgcatc tggtagatag cctaattcgc gatattgttc gataaattgc aagattgatt
    16741 cgtcacgttt gcttagtttt ttacctgttt cactgttgat gatcaatgtc atatgtccga
    16801 aggttggtgg tgtccagcca aatgcttcat agatcatcag ttgttttggt gtattggcaa
    16861 tatggtcatc tccacgtaaa acgtgagtga ttttcatcaa atgatcatcg accgcaacag
    16921 caaaattata agttggcata ccatcgcgtt tttgtatgac aaagtcgcca ccgatattat
    16981 cggattcgaa cgaaatagcg cctttcacca tatctgtaaa tgcgtattct gtattgcgag
    17041 gcacacggaa acgtacaaca gattctagac cttgtgcttc tttttctgct tgttcttcag
    17101 gtgtcaagtt cgcacatgtt cctgcgtaat gaggcatttc tcctcttgcg cgttgtgctt
    17161 cccgttctgc ttctaattct tcttctgtgc aatagcattt gtatgctcga ttgctagcaa
    17221 gcagctgatc aatcagcggt tgatagattt ctttacgttc agattgacgg taagggccgt
    17281 attctccagg attttcagga gattcatccc aattcattcc caaccaagca aggttttcca
    17341 actggctttt ttcgccatct tcgatattac gtttttggtc agtatcctca atacgaataa
    17401 taaagtctcc atcattgtgg cgggcaaaca aataattgaa taatgcagtt cttgcatttc
    17461 caatatgcag gtgcccagtt ggacttggtg catagcgtac gcgtactttc gtcattttct
    17521 tcctcttctt tcaaactaga ctttgactaa cagcaaaaat tccgtcagtt acggcttctc
    17581 aaatatcatt tatttaaagc cactcagcac aaattttacc ttttctaagg taaatagtaa
    17641 agccaactgg ttgaatttcc ttaccagttg gctgaatttc ttttttattt tgattgttta
    17701 gtctttttct ctgtctcttt tttattgtcg attcctcgtc ctgcatgtgc aggtttagca
    17761 aagatcatgc gtccagcagc tgtttgaagg gcacttgtta caacgacttc gatacgttca
    17821 ttcatgtaat gctgtccatc ttctacgaca accatcgtgc cgtcatcaag gtaggctact
    17881 ccttgttggc gttctgtccc ggcttttact actagaacat tcatcgtttc accagggatg
    17941 acgactggtt ttacggcatt tgccaacgca ttgatattaa gaacaggtac attttggaat
    18001 tcagagactt tattcaagtt atagtcattg gttacaatga caccatctaa aagcttagcc
    18061 aatttgatca atttgctgtc tacttcactg atatcttcaa agtctccatc gtacatttcg
    18121 acagatatac cctcttcttt ttgcaaggca tttaggatat ctaatccgcg acgaccacgc
    18181 acacgtttca aactatcccc tgagtccgcg atgtattgta gttcgtaaag aacaaaatta
    18241 gggatcatca aggttccttc taagaatcca gtctttgtaa tatcataaat ccgaccgtca
    18301 atgatcacgc ttgtatccaa gattttatat ttatggaaat tttctcctga ttttcgttct
    18361 aacagttgac tctcaacttg actagctgtt tcttgctcga tttttttgct tcgtgaacca
    18421 aaaactttct tccattcttc gattcgtgtc gttcccattc tcaatcccag atagcctaag
    18481 atgatcatca gtaaaatcgg caatacacta ttgacgaatg gaatttccat attgtaaaga
    18541 ggggtcgaga tcacaacacc taaagtcaat ccgatgatcg caccgatcgc accgaagaga
    18601 agataagtca agctgacctc acttagtttt tgttctactt gtttgataat agctgagata
    18661 tgtttggcca acagcaaaga taataaaaag aaaataagtg cgcctaacag gctgttggta
    18721 aatgtattat tcagccatcc gtttgactgt tgaccgattg cctgccaagc tgagggtaaa
    18781 aatgaaatac ccaagcttgc gccgacaatg atcatcagca aagtaatgac acgtttttgc
    18841 atcgtttttc ctccgtttcg acaatcattt attagttagc ggaacacacg cttcaacgtt
    18901 tcagccaaag ttgctactcc aactatctcg attccttttg gcgcttccca gttacctaag
    18961 ttgtttttag gtaggtatac ttttgtgaag ccgagttttt ggacttcttt gactcgttgt
    19021 tcaatatgac tgacacgtct gatttctcca gtcagtccaa tttcaccgat gaaacattcc
    19081 gttggctgtg ttcctttttc tttatagctt gaagcaatac tgacagcgat agctaaatca
    19141 atcgctggtt catttaattt taccccacct gcagctttta agtaagcatc ttgattctga
    19201 agaagcaacc cagctctttt ttccagcact gccatgatca gagatacccg attgaaatca
    19261 agacctgttg ttgttcgttt ggcatttccg aacatagtag gtgtgactaa tgcttggatc
    19321 tctaccaaaa ttggacgtgt cccttccatt gccacaacga tagcagagcc agttgcaccg
    19381 tctaggcgtt cttctaagaa aatctgtgaa ggattagcga cttcttccaa tccatgctca
    19441 tgcatttcaa aaatcccaat ctcattcgtt gagccaaatc ggttttttac tgctcgcaaa
    19501 atccgaaaac tatgatgttg ttcaccttca aaatataaca ccgtatccac catatgttct
    19561 aacattcttg ggccggcgat cgagccttct ttggtaacat gaccgacgat aaaaatggct
    19621 atcccatttg ttttagcgat tttgagtagt tctgcagtgg tttctcttac ttggctgaca
    19681 cttcctgcca cacttgtgat gtctggctgc gtcatcgttt ggatggaatc aatgataaca
    19741 taatcagggg agatatgttc gatggcacga ctgatttcgt tcatatctgt ttcagcgtat
    19801 agataaaatt ctgtgttgat acttccgagg cgttccgcgc gtaattttat ctgctctgcg
    19861 ctttcttcgc cggatacata taagactttt ccgcctatgg ctgctaattg ctgagaaact
    19921 tgtaacagca aagtggattt cccgatacct ggatcgccac caatcaagac catagaccct
    19981 ggcaccacac ctccgcctaa cacacggttc agttcttcta attttgtttt gatccgaggt
    20041 tcttttttag ggacgacgtc tgcgattttt gttggttttg ctttttctcc tgtcaaactt
    20101 gtccgagttc tgcggtccgt tgtatcttgt tcgatttctt cgaccatcgt attccatttt
    20161 ccgcaatttg ggcagcgtcc caagaactta ggcgacacat acccgcaatt ttggcaaaca
    20221 aattgtactt ttgatttttt tgccattgta ccgcctcctt ttatcctaat catgagacat
    20281 taaatcaagc ttaagtatct ttaacgtttt atttactgga agaaccaaat ccgccttgac
    20341 gttgagcagt cgtttgatct tgatctgctt ttaaaaacgg caaaaagatc ccttggccga
    20401 tacgctcgcc ttttttgatc actacatcct tgaaaccaaa gttggtgaat tggaacatga
    20461 tatggccttc atttgcctca ttattgtaat aatcactgtc aatcacacct actccgtttg
    20521 tcaacactaa aaaccgcttc aacggattgc ttgaacggtt agcaagctgc aaaaattcat
    20581 cttctcccat gtaagctttg attccagtag gcacgagggt tggtttcaat tgtttttcat
    20641 tttctgatac atcttttttc aatccatctg cgactgtttc caaccccttt ttccagcctg
    20701 ctttccagat acttggaatc acgacctctt ctgctgcttc aaaatcatag ccagcagcat
    20761 gatgtgtagc gcgctgcggt agattgatat tttgatcttt atatttagaa ataacttcaa
    20821 atcctctttt tctcaccggt tttcctccct aaataaacta ctcctattct aacatatccg
    20881 aggctgtaaa agactttctt tacaaattct tcaagaaatt ctgctatctt aaagtaaacc
    20941 attaattcgt tctttttcca aacgactgca agttgtacaa aaaaactatt ttttacatga
    21001 ctttactcgt caaaacaagt agaatagaac gaaattaatg agtggatatt tgattgaggt
    21061 gtcacatgcg taaagaaatt aaaaatgcaa aacgaattat tgtcaaagta ggaacaagta
    21121 cattgattta tcccaatgga aatatcaatc ttgcggcaat cgatcagtta gcttttgtcc
    21181 tttctgatct tagaaatcgg ggaaaggaag tgatcttagt ctcatcagga gctgtaggcg
    21241 ttggtatgca taaattacat ttgtcagaac gtccgacttt gattcctgaa cagcaagctg
    21301 cggcggctgt tggtcaagcc gaactgatga atatctataa tcaaagattc agtgtatata
    21361 atcaacagac ggcacagatc ctcgtgacaa gagacgtggt agattatcct gaaagtcgta
    21421 aaaatgtgag gaatacattg gaacagcttt tgattatgga cacgatccct atcgtgaacg
    21481 aaaacgatac tgtagcagtg gatgaattag atcatctgac aaaatttggc gataatgatc
    21541 agctgtcagc catcatggca aaacttgtcg cggcagacct tttgatcgtc ctttctgata
    21601 tcgacgggtt ttattcagat aatccgacta caaatcccga ggctcagatg tatcgctata
    21661 tcgataaaat cgatgaaaag attctggcac aagcaggtgg tgctggtagc acatttggaa
    21721 caggcggtat gcagagtaaa ttaaaagccg cttctaggat atttgaagca aacagcgcaa
    21781 tgattttagc aaatggaaaa aatccggcaa ttatttttga tattcttgca ggaaaagaag
    21841 taggtacttt atttaaggag gacttgaaat gactgacttg atgcaattag gacaacaggc
    21901 aaaagatagc gcgcaacaac tagcacttat gcctacaaac cgcaaaaatg agctacttgt
    21961 tcaaatggca caaaccatca aaaagaatca acaagcgatc attgaagcaa atcataaaga
    22021 cttagaaaaa gcagtcgaaa acaatatcag tgaaacgatg caagaccgct tgcagctgac
    22081 acctgagcga atcaatgcca tggctacaga gatcgaaaaa attgcttcgt tagacgatcc
    22141 tattggaaaa gtagatgaga tgtggacgaa caccgacgga ttgagaatcg gcaaaaagcg
    22201 cgtgccacta ggcgtgaccg gcatcattta cgaatctcgt ccaaatgtca cgacagatgc
    22261 agctagtcta gcattcaaat cagggaatgc cgttatcctt cgcggtggca aagaagcatt
    22321 cttctcgaat caattattag tccaattatt gcagcaagta ctggtcagtg ctggcgaatc
    22381 tccttatgcc atccagtttg tcgatgatac ttctcatgaa actgcaggac agttgatgcg
    22441 attgacagaa tacttagatg tgctgattcc tcgaggcgga gcaaaattaa tccaacgagt
    22501 caaagaacag gcaacggtac ctatcattga aacaggtaca ggaaataatc acgtatatat
    22561 cgataaagaa gcacagttat ctatggcagt gaacatcatc gtcaatgcaa aagcttcccg
    22621 tccatctgtc tgtaacgcgg cagaaacatt gctgatccat tctgagatcg cacctttttt
    22681 cttgccagct attgaaaaag aactggtaga acacggggtc tctttacgtg cggatgctcg
    22741 tgcgttagaa tatctagaga cggctgcttt agcagaagac acagattggg atactgaata
    22801 tctagattat atccttgctg tcaaagtggt cgattctttg gatgaagcaa ttgcacatat
    22861 caatcggcat aatactaaac attcagaaac gatcgtgaca gatagttatg cggctagtca
    22921 acgtttctta aatgaagtgg acgcggcggc tgtctatgtc aatgcctcca ctcgttttac
    22981 agacggatcg gtatttggtt ttggtgcaga gattggtatc tctactcaaa agttgcatgc
    23041 ccgtggtcca atggggttaa atgaattaac ttccacaaaa tatatcattt atggcgatgg
    23101 acaaatccgc gaataatgtt tcgtgtgaaa cattattccc gccttttgcc ttttttctgt
    23161 aaaggttcta tccaactaga aaggtgtcaa tatgtttgaa attttaatga ttttttttat
    23221 ttaccttatt tctctgaata tcgctgcttt tctgggagtc ggtattttat cccttttctt
    23281 tcaattcaaa aaacgaagca tagggagtca acgggaaaaa tggtcgcagt attttgacaa
    23341 aatcgggcaa aaagggctgg ttacacgctt gcacattagt tacatggttg cattatgctt
    23401 attagcgaca atcaattact attctttttt cgatcattca atcgcttata cgattacgct
    23461 actgattgct gggatttttc acttatctta taaatatcag ctgaataaaa accacttgaa
    23521 tcggactttc cgctaaaaaa tatccgtacg ataagtagag tgccttgaag atcggcaaaa
    23581 aactgccgga ctttttagag gctgtgacaa agattttgtc acagcctctt tttccatata
    23641 aacggcgttc gcctaccagc tgcctggtaa atctgtttcc tcttcgttaa gaagcgaaaa
    23701 tccgtcaaat gtttcgccat cataataacg aagcagctca tccatggaca agttcatcgt
    23761 tttttctttt tcttcttccg ctgattcagc cagtagctgt tcccgataca cctgatacat
    23821 accagctatt ttttgctgca tcgcttttct agtttcacct tccgcattga ttattggcaa
    23881 ttctttgata tagccacgat aaatcaaccg atcatctgaa aattcaatgg gtaattcaac
    23941 gagtgtcaac ccgacttcct gaaaatattc ttccagcatc tattatcccc ccattctttt
    24001 tcttaaatga attttagctt acctactgaa aattttccag tttcattatt ttgagaaaaa
    24061 aagggaatgt gacaaaaatc gtgtcacatc ccctttttcc gaataaatgg tgttcttctg
    24121 ccaactcctc aatcataagt cacaatattc aacaaacatg agaaactgtt ttttgaaatt
    24181 gttccttgtg attcagagtt aaacgacagt ctcacaacct cttggtaaac ggcgttcaaa
    24241 agcttgcatc tgcggtaata agcccaacca aacgaaaaat atgagaagct atttttgttt
    24301 ggctgttctt atgacttgat gcaggacagc tttttcacaa cctctgattt cttattcttc
    24361 ttcatttgta gcaaaataat tacggtgata aatgatacaa catacactga taagaacaat
    24421 ggcacctgca aaataaaacg catagctttc accaatatgt tcaccaatca cacctgcaat
    24481 aaatggtgct aaagctccac cgaaaaagcg gacaaaacta tagctggacg aagcaacgtt
    24541 tcgttgcaga cctggtattt ccatagcaat cgtcgtcaac agtgtattga taattccttg
    24601 gaaaaatcct gctaacacaa tgctgatcgg tacaaggact ggcatagaag ttccagctcc
    24661 taataacagc aaacaaataa tgaataagac aaatgctgct aaaattgtcc gataagtcgt
    24721 aaacttccgc tcaagagccg gtgcaacgaa aatcgaagca atagccagaa gcacacccca
    24781 gccaaaaaat acaaaaccga cttctaattc agaatagcca atcaataaaa atggcgcata
    24841 cgcaagtaaa gtaaagaatc cgaaattata taacaatgcg attagtccaa ctgaacgaag
    24901 tttatgattg cttaacgctt tgacaccttc ccaaaggcct acttttctct ttggtttctc
    24961 agtttcaggt aaaagtaccg ctagggctat aaaaccgcaa aacatcaaaa atgctacccc
    25021 aaaaaatgga taacgccatg aaccactgcc caagacccca ccgactagag ggccaacagc
    25081 cattccgcag ccaagtgctg cttcatacat catgattgct ttttctgtgt taccaactaa
    25141 aatactgacg atcgttgcta aagcagtaga gacaaataaa gcattcccga caccccagcc
    25201 ggcacgtaac ccaatcagca ttccgatatt cccagataat ccgccaagac cagcgaatac
    25261 aacgatgata aacaatcctg tcatcaatgt tttttcaaac cgatccgact agataagaaa
    25321 cctgtaaata gcatcaccac accagtgact aacatgtaac tagtaaataa cagggtcgtt
    25381 tcagctggcg tagcgtttaa ctgtgcagca atcgttttta agattgggtc tactagaccc
    25441 actcccataa aagcgatcat acaggtagct gctacaacta gcgctgaact tttccccttt
    25501 gtttcttcat ttgtttgttc ttgtatatct atttgattct ccaatatttc catccctttc
    25561 tctctattag catcatacat gatttatttt acatgtaaat atgcacatgt aaaacgtgga
    25621 aactttactg tgatataata aagaaaataa gacgagaggt aggattttta tgaatacttt
    25681 agcctatata ttattatgcg tgcttttatc caaaccacgt tctgggtatg aattgaagca
    25741 gctgatcaca attttttggg aagcccatca cagtcagatc tatacaacgc ttgccaagtt
    25801 agaccagcag ggttatgtga ccgttttgga caaagacgag cactctcaaa aaaagatcta
    25861 ccacttgact gatgaaggac gtgctcttgt agaggaatgg atcaaagagg aaactcccgc
    25921 tcctacgcaa aaagatgaat ttctagcaaa aatctatgct ttttcgacgc tcgataaaac
    25981 aacagctact ggactgctat tcactagaga acaacagctg aacaaaatca tgcaaaaaaa
    26041 tcaaaaaaaa ttgaatgacc tctcgctaga caagaaacaa gaatttggac gttatgttgt
    26101 gatccagcgc agaattatgc tttgtcagca ggaactccaa tggtgtcagc aagtaagaga
    26161 acaaatgcag cagttttttg agtaagggat aaacagaacc tttctagaaa tatctattga
    26221 agagcaaatt atcttcaata aaagagcaat acagttggaa aagaaaaagc gatccgaacg
    26281 acaggccaaa acctatcgtt cggatcacta tttttctaga actgcattca agagactatg
    26341 acaaaaagca tgccaaggcc ttttttcaaa taacagtgtt ccatcattca ttactttgaa
    26401 ttgcttttag cacacttctt gaaagcgggc caacgatgat caactgcaac ggtaaagcgg
    26461 caatcacatt cattttccaa gcattcatat aagcagagaa taattgatca ggtattcctt
    26521 gttccatgat cacaccgaat aaagacatac atgtcaccat tcctaaaacc atcaagcatg
    26581 aaatagttaa gatcatttgg accttgcttt ctggatttac tggtagttta aaggcaattt
    26641 ttttcgccag cacaccgatg atgaagacat ccaaaatgaa agccaccacg aatccaggca
    26701 ctagaccaat caacaaactt gataaatgaa gatcttgatg cagccataaa ttatacgcgc
    26761 tcatccccaa gaccatcaaa aaacacatca ttgttgtaaa aattattcct tcttttttat
    26821 ttgtaggcaa tctgtaccac tcctttaaat ttgtcacgaa aatcatcgta gcacatttaa
    26881 aaatttctcg taagtacttt ttttattttc tgccctttta tttcataaac ttcatcagcc
    26941 gtacgctcga caaagtaaga atcatgagaa gtaaacaaaa tcgttcccgg atatatttgc
    27001 attagcttct ctaaagcttc gatcgttgcc atatcaataa aattagtcgg ttcatctaac
    27061 agcaacacat tacttggctt agtaaaaagc aaagcaatcg tcagacgggt cgcttctccc
    27121 ccacttaatg tacataacgg tttcgtcaaa gtttcctgag caaaccctaa gtgattcagc
    27181 aaactacgga caagcgattc tgaaaactct gtttgtctca tgacaaaacg aatgatggat
    27241 tcttcagatg tcatttgata acccttttgc tgatatactt gaaagcttac tttcggagag
    27301 agaaggattc cttctccttg tttgcggata tgttctaata aggttgtctt acctgaacca
    27361 ttttcaccga caatcgcgat ttttttacct aaagaaaact gaaaatcacc atttaccagt
    27421 aatgttctcc cacttctttc caattggaca ttttgtccca tgatgggata gcggctgtgg
    27481 agttcaagag attttggtac tggaaaacgg atttgtttca tctgctgcgg cttggttgtt
    27541 tctcctattt tttggagtct tctctctaat gttttcgcat tcttttggat ggctttttgt
    27601 acactgtctt tttgtttaga ggaagacaac cgatccggtc tgatttgttg ctttttcttt
    27661 ttactggaca ctttttccaa cttttccgct tgttttttct tttcttggat cgatttttcc
    27721 aatcgttttt tctctttcat gatcgactct gcttctcgta gctgagtctt tttctccaat
    27781 tccttttgag tgagataggc actataattc cccgaaaact ctcggatttc tccgtctttt
    27841 acttcccaga ttttcgatgc tagctcatct agaaaatagc gatcatgact aacaaaaacc
    27901 agcgtaccat aataataacg aagctcttca atcagtcgat caatactttt tctatctaga
    27961 tgggttgttg gctcgtctag aagcatccct tgttcataag aagaaagaag acgtatcaac
    28021 tcgatctttc gttcttcccc tccgctccat ttccgctctg aagtttcttc cagtcttaac
    28081 tggaaatgac tggctaattc caaatttagg tcctttgctt cagcgagata ggttaattga
    28141 ggcaggtagt tgaaggtgat ctctgtttgg attttcccat gatcgggaaa aagttcaccg
    28201 gctattagtt tgagcaaagt cgatttaccg acaccatttt ctcctatgat ccctatacga
    28261 tcttgctgat agacacgaag agaatcaata gatagttcta gctggttgcc aaagctgact
    28321 tgtagatttg ttatatttac tgctaaattt tccatatgtt ttacactcct atttcatgta
    28381 aaacaacaat tatcgatact aaaaaagagc agaaaaatct gtttgcaaaa aaactgcatc
    28441 acatcttttc ctactctgaa cgaaataact atgactaaat caccaaatct gtcatagctt
    28501 tcacctattc tactgtcgtt cgaaaaaatg gacatactcc atccatgaaa ttgttgttta
    28561 tacatgtctt ttgaatttat ttagttgtta ttcaaaagtt cgaaacgtaa tttcatcgat
    28621 gcagtcattt ttccgacctc cttttttatt aggttaccac acacgattca aaaaaagcaa
    28681 tagctactga atagtaactg aaaacctttt attcgaacgt agtgatcttt tctggtgcta
    28741 aacgaacaga ctcgtaacct tcttctaccg cttttcctat tgataaaata ataactggaa
    28801 cataacggtc tttatccaaa ccaaatgcct ctgccaactg atctgcttcg aatccaccaa
    28861 ttggatttgt ttcatagcca tgatcacgcg caactaacat gaattgcata gccgctaagc
    28921 tagaatcgac tttgactaca tcgttcattt cttctcttga aaaactcttg taataaggaa
    28981 tgatcgcagc taattgttga tctctgactt cttttggcat cttccctgat tcgtaagctt
    29041 ggttatagat ttcttcgcca tattcgtaac attgcatatc gccaaaaatc atgaccattg
    29101 cagaagaagt atcattttgt ctcgtattga aacggatcaa aggacgcaag atttcttttg
    29161 cttcctcgct ttcagcgacc acaaagcgcc atggttgcat attgacagaa gaaggtgcag
    29221 tcgtcgcttc tttgatcatt tccagcattt cttcgtgtgg gattttgtat gtttcatcat
    29281 atacgcgaac ggatcttcta ccagtcataa ttgttgaaaa atcatttttt tgttccatta
    29341 ttattaacct ctttcgtttc tgttgttata ttgcaaagtt gggtattcaa tttatctaaa
    29401 agtgttagaa attgtttcat ttctacatcg ctgaaagccg gggatagcac ggaacctgta
    29461 tctttatggt ccatttcaca atgctcgatt ttcttctggg ctttttctgt tagcgctaca
    29521 tacacttccc ggttgttctg aggatttctc tgacgcgaaa catagccatt tgcttccaat
    29581 accttcagat gacgagtaac tgctccttga tcaatctgca acttttcttg caaactgctt
    29641 tgtagcaatg gattttcttc tttcagtctc atcatgatct cgtatctagt aagacttata
    29701 ccgatatttt cttcaaagat ctggacgatt ttttgatcgg ctaatttgat ttgatataaa
    29761 aggtgactaa tctcttctaa agacataatt tcctcctttc taataattga ctcgtcaacg
    29821 attgacttgt caataataga ataaaaaaag aaaaaagtca aagaatcagc tgtgtaataa
    29881 aaaaaacgct ctcaatagaa aagtatagag aaaagaaatt ttttgtagaa tgaacggaaa
    29941 aaatgataaa gaaagcacaa cgctaaaacc aagattattg attcttatca aactatagaa
    30001 gatagctctt ctgcagtaga aaattttaca ccgccaaaaa tttccgaaat cgccttttgc
    30061 aagaatcccg tcgaatatta gaaattggat cagtatgctt aaacagtaat aaaaaagtta
    30121 tgacaagatg ttgtcacaac cttttttgag taaacggtgt tcaaaaactt gcatctgcgg
    30181 taataagttc aaccaaacaa aaaacatgaa aatctgtttt tgtttggctg ttcttactac
    30241 ttgatgcagg gcagcttttt cactacctct ttttaataac cataaaatcc tattccttca
    30301 tatctccagc ccatcggtgt tagccaatct ctttccttag cactcaaggt atagtgatgg
    30361 gtaccagttt tttcatttgg attaaatagt cgataaattg gcttggaacc accagaataa
    30421 aaacttagac cttcataacg ccaaccgatt tttgttaaag agttgatctc agattgtgcc
    30481 attgtgtaat gatggtctcc actgtaagga ttgtataaac ggtagacagg ttgtccgctg
    30541 tttggtgctt gccaagcaat cccttcatat tcccagccta cagttggcag ataatttttt
    30601 tctccggcat tcaatgtata taaatgttct cctgtatgac ggttatacag acgatacatc
    30661 ggagaagttg aagttggtgc tgtatcatac tgtgtcagat tataggtttc gattacattg
    30721 ttcaatgtag aaccataatt aggcgcagtg gcataacgac ctgttagcca agccgtagca
    30781 tctttgtaag agttcgtatt gcttttccat gcaccggaat agtagtaaac gcctggctga
    30841 aaagaagtcg tcttcaatac cgttacgtta tcttgcaatg attctttgta cgaaggatat
    30901 tttctaaaag cggcattcac cgtaatccat tgcccattca catattccga agtaggcatc
    30961 gtaacagatt ggccattata gcttccttta atcccaaata gattgtaatt aggggcagaa
    31021 gccaaagtac tgttccccca gccactttca acgatggctt gagcaatcat gactgatgca
    31081 tataagtcat tggcacttgc caaactttgt gcagttgagg caatactatc aatgaacgct
    31141 tgttgagaat tagcagatga gcgagcataa aaagcatagc cagtatagtt ttcgaattca
    31201 gacaaatctg cttgatgggt atgtgtcaat gtttgcccat ctgtcaattg gttcgtgatt
    31261 tcttctgcat tctctggatt gttctgagct tctgctgatg tactgtcttc ggtttgtgtt
    31321 tgttctgttg tttcagttgt tggttcttcg gttgaaattg tattttctga gacagtagct
    31381 gactgcaaag tactctctgt atcgttttca attcctgttt ctgaagaact cgatgtctga
    31441 gttgcgacag tttctgtcga ttcttcagct agcactgttg tgttggcaac tgctagattt
    31501 cctaaaagaa taacgctaaa taataaattc tttgctttca tcttttttct cctttgaatt
    31561 atgaaacttt tcctaaataa cagtgtaact aaaaaagtaa aagcaagtca atgtccatat
    31621 ttaaataaca tttgtttctt tttgataaat ttgctttttc tacataaaaa gacgtaaatg
    31681 aagaatattc ttctccattc acgccttaaa ttgatggtcg tttgaataac tatttaacaa
    31741 gtaattctct ttggatatta tccgttatgg attatacttc ctgtgtcgtc ggacggataa
    31801 tcatgttact gacagacatc cggtctggcg tttctatcgc aaatgctaca gcagaagcta
    31861 tatcttcgga ggtcaatccc cattcttttt gcgcttcatg aagttcaaga gcagcctttt
    31921 tgtcgctgat ggtttgatat aattcagttt gaacagctcc tggagagatg atcgtagatt
    31981 tgatgttatt ttgccgttgc tcttgacgta atccttccat gattgcacgg acagcaaatt
    32041 ttgttccgca ataaacggcg gaatcaggat aaacaacatg tcccgccaca gaatcagttg
    32101 aaatgatatg cccagatttt tgtttttcca tgattggaag tacggcggaa attccattta
    32161 acacacccat gatgttgata tcaagcatat tttgccattc atcacggtga ccttcaacta
    32221 atggagcggt tggcatgatt cctgcattat tgtacaatac atcgattcga ccgaactttt
    32281 ccatcgctaa atcgataact gcttggactt gtgcaaaatc tcttacatct gctgtttgaa
    32341 tatatagctc agcttctggt aaagattctt ttattgcttt taggcgatct tctcggcgtg
    32401 cagctataac tagtttagcc cctttttctg ctaatagctt cgttgttgct tcaccgattc
    32461 cgcttgaagc gcccatgatg acaatcactt tttctgataa atcattcatt ctattctcct
    32521 cctgttttga aaaatctctt gcttatactc actatctctt atttcatctt actgttcaaa
    32581 taatccgttc gacaggtttg acgattttgg cagggattcc tgcaacgatg gtattgtctg
    32641 ggacatcttt cgtaacagtt gcattcgctg caacaatggc attctcgcca atcgttacgc
    32701 ctggcagaat agttgctcct gctccgatcc aagcattttt cttgatgtgg attggcttta
    32761 ctgacaagcc tcttcgttct tttggatcgg ttagatggtt caccgtgatg aggttgacac
    32821 gaggaccaat caatactgaa tcttctaaac agatgccacc taagtcaacg aacatcactt
    32881 gacgattgat aaagacattt ttccctatac taatatgttt gccgaaatct gtataaaaag
    32941 gcagcagaac agtcaccgta tcatctactt tgtcactaat gatattttct agccgtttgc
    33001 ggatatctgc tggttcatgg tacccagtat tcagcgctgc taaaggctct tcgttttcag
    33061 cttggatctg atggatcact tcaaagagcg gatccgttac tcgaatctct ttattgacga
    33121 tttgtttttc gatgaggctt gtcattttgc tccctccttt tatccttact ttataacgga
    33181 cggcttatca tgtctaatac ttataaaaca tacttaacta ttccttttaa ctatacttag
    33241 tggtatgctt ttttaaaaac aggagggaaa aaatggatat tcgtatactg acgtattttt
    33301 tagcagtagc tagagaaaaa aatatcagcc gagcagcaaa atctctgcat atcacgcaac
    33361 ctactctctc aaaacaactg aaagatttag aagaagaact tggtgtgaca cttttcactc
    33421 ggggctcccg agagatacag ttgacggatt ctggtcgata tctttattca aaaggaaaag
    33481 aaatcttgtc gttggtagaa aaaacggcga acaatttaac aaatgacgca atcatctctg
    33541 gagaaatcta cattggcgga ggcgaaacta aagcaatctc tttcatttct gatagccttc
    33601 attcgttgat caaggcgcat cctgatatcc agtttcattt atatagcggg aatgcagatg
    33661 atatcataga caaactggat agcggattac ttgattttgg actcgtaatc gaaccgacaa
    33721 acaaacaaaa ataccagtca tttcgactgc cagcccaaga tcgttgggga cttttgatac
    33781 atcgaaatca tcctttagca aagcaaaccg tcatccatcc gcaggaactg aaaactgttc
    33841 ctttgatcat ttcccggcaa acgtcagtgg acagtcaact agctgaatgg ctaggtttca
    33901 gcatagatca gttatcgatc gtcggaacat ataacttatt gtacaatgct tctttgatgg
    33961 tcgataacgg gacttttggt gcattagctc tagatgggat catcaatact tatgggacgg
    34021 aaaaaatatt catcccactc gatccgccat tacaagcttc actgaatctc atttggaaaa
    34081 aagaccagcc tctttctcct gctgctcgta aatttttaga tgtcctaacg gagaaataca
    34141 gttaagtaga gtttttttcc ttcgctttca gctttttaac aacgaataag ataagcaaaa
    34201 gatccaacga ttaaataaca taagtttctt aatttatttc ttccttttat ttctgataat
    34261 tccagtaaat tactataatt ataaaaaaag aattgacaaa acataactgt ctatataaaa
    34321 ttgattacgt taacaataat tcgtttcatg tgactagatc atactaggca tgaaaaatct
    34381 ctgaggaaca tggtttccct aggaggtttt tcgtgtctat ttttgtttgg ccgcaatcaa
    34441 ctggtctagc aaacgtgaaa accaaatctt caaaaagggt gcgcgcatga aaatcaaaaa
    34501 gatctttaat caaaatgcag tattggtaga tgacggtgga caggaaaaag tagctattgg
    34561 aaaagggatt ggatttgaca gaaaaagaaa tgatttgatt tttgatcgtg atgtcgaacg
    34621 gatatttgtc atggaaccag aaggtcaaat caagttgcaa aatttactta atcagattga
    34681 tgaacgcttt ttatttgcag cagaaacgat cattgatcat gcggaaactg ttttgatgga
    34741 aaagttgaat gaacatgtat tgatcgcttt gaccgaccac attgcttttt ctgcggagaa
    34801 cattaataac ggtatcgtca tccgcaacaa acttttaaga gaaatcgaag tgctttacag
    34861 tgatgaattc agtattgctc aatgggcagt agagtatctg acaaaagagc ttgggattcc
    34921 atatacttat gacgaagcag gatatatcgc aatccatatt cacagcggac gaagcggtcg
    34981 gaacaataac catcgcagta tccgtgaagt gacgattgta tctgacatca tccatttagt
    35041 ggaatcagaa ttggaaacag atatccattc agaaccattt tcactgaatt attcaagact
    35101 ggtgaatcat ttacgactgc ttctacaacg aacccatgcg caacaatatg ctgttttaga
    35161 cacggaaatc gtagaaatgg tcaaacgaaa atacccagaa agctataaga tttctaaaaa
    35221 aatccgtgtg ttgctgacca aagaatacca gctcgcgatc acgaaagaag aactgggtta
    35281 cttggccatc catatcgagc gtttaagaag cgcaatcgta caaacaaatg ttaacaatca
    35341 tgaggaggaa aaaaattgaa agcatatcta caacgaatgg gacgttccct tcaacttcca
    35401 gttgcggtct tacctgctgc tgctttacta gtagggatcg gaaactggtg ggcatcttat
    35461 agtaacgaca ttgtcgctca ttttttacaa gctggcggta acgcagtttt aggtcaatta
    35521 cctttgctgt ttgccgttgg gttagcacta gggatgtcag tggacaaatc aggtgctgct
    35581 gccttggcag gtttggtcgc atttgaagtt ccagtaaatg ttttaaaaac tgattcagta
    35641 gctactttat tgaatatcaa accagaatca gttgatccat cttttgctca gatcagtaac
    35701 gtatttattg gtattattgc cggattgatt gcggcagctc tttataatcg tttccacgaa
    35761 gcaaaattac cgatggcttt atctttcttt agcggcaagc gtctagtgcc aatcttggct
    35821 gcctttgtca tgttgctgat cagtgcagtg ctactgctcg tatggcctcc ggtttactct
    35881 gctttagttt cttttgctaa attcattttg agtttaggct ggggtggtgc aggtctttat
    35941 ggcttcttca accgtttact gatccctaca ggattgcatc aagcaatgaa ctccgtgttc
    36001 tggtttgacg tagcaggaat caatgatatt ggtaaatttt tggcttctga aggaacaaaa
    36061 ggcgtaacag gaatgtatca agctggattt ttcccaatca tgatgttcgg gcttccagca
    36121 ggagcatatg ctatttacag aaatgcacgt ccagaaaaca aagcaaaaac agcttccttg
    36181 atgctagcag cagcttttgc ttcattcttt actggtgtga cagaaccact tgaattttca
    36241 tttatgttcg ttgcttggcc attatatgtc ttgcatgcaa ttttcactgg gatttcttta
    36301 gcagtgagtg cattcttcca ttggacagca ggatttgcct ttagtgccgg atttgtcgat
    36361 tacttcctat ctttgaaaaa tccgattgcg aatcagccat tgatgttgat cgtccaagga
    36421 ttagtaacag cagtgattta ctactttggc tttaactttg ctatcaaaaa attccatctg
    36481 atgactcctg gccgcgagga agcagatctt tcagatgacg atactgcaac gactaattca
    36541 gacaataaat atgcggcaca agcaagcaaa atctacgctg cattaggcgg agccgacaat
    36601 gtaacttcga tcgataactg tactacacgc cttcgtttgc aagtcaaaga tacaggcctg
    36661 atcgatcaaa ataaaatcaa agccactggc gtacctggta tgaagatcat cgacggaaaa
    36721 aatgcgcaag tcatcgtagg tacagaagta caattcgttg cagatgagat ggcaaaacta
    36781 catggtggtg ctgcagcacg acctgcacaa acaaacaccg tggttaaaac agaaacattt
    36841 gccggtgaaa cgatcgaaca agatatttac gctatcgcaa atgggaaact gattccaatc
    36901 actgaagttt cagatgatgt attcgcggaa aaaatgatgg gtgacggcta tgctgtatta
    36961 ccagaaaatg gagaaatctt ctctccaatc gctggtacga tcacaaacat tttcccaacg
    37021 aaacatgctg tcggaatcca aacagatgct ggtatcgaag tcttgcttca tatgggcatc
    37081 aacactgttg acttaaaagg cgaaccattc actttatatg tagaagaagg tcaaaaagtc
    37141 gcaagaggtc aattgattgc tttagttgat cttgccgcta ttcaatctgc aggcaaaaac
    37201 acggatatgg tcgttgtatt cacaaatggc gacaaagtcc aaagcttaga aatcgaaccc
    37261 gcacgtgatg taaaagcaaa tgataaaatc ggctctgttt ccaacaaagc ctgagccatc
    37321 gtcttcccca gactttttta tcgataaaca aacaggagct gagacaaaaa tcttgtctca
    37381 gctccttttt ccgaataaaa acggcgttct cctgccaact ccgcggtaat aagtccagct
    37441 aaacgaaaca tatgaagagc tattttttat ttgactcttc ttattacttg atgcagaaca
    37501 gctttttcac aaaatttttc ttcatactca aaactaatcg gctttttcac aacctcttta
    37561 tacattattc ctgatccaat atttctttta atgctgcttc aagagaagtt aatggttttc
    37621 ccaatacttt ttctaaatca tcagaggaaa tagctaactg atccttttta atatcatgtt
    37681 ggatcgctaa aaacatgtca gctgcttctt gagggaatcc ggcttctttt agattttcga
    37741 caaacgctgc atcacttgct tcaataattt ctaactcttt acctgtcgca cgctctaatg
    37801 ccttggctaa ttcttcatag tcagttagat tgccggataa ttctaagatt tctggagagt
    37861 cactttctac tagtactttt gctgcagctt ctgcatattc acgtttcaat gcccaacctg
    37921 tttttccatt tcctgcagca taaacgaatt gaccagtttt taaagcctgc ccaatgatag
    37981 gtaattcatt ctctacatac caattattcc gtaaaaatgt atgggaaata cctgaatcct
    38041 taatcatttt ttcagtgaat tggtgatctg gcgctaatac actgatcgag tgatcggcat
    38101 ctgcaaaact tgtataagcg atataagata ccccagctgc ttgtgctgcc ttcactacat
    38161 tcttatgttc ggcttgacga tttcctggaa cgccagagac aaaaagcaat cgatctattc
    38221 ctttcaacgc ttctttcatt gagtctaaat catcataatc accaatacgt atattaaatc
    38281 cagcttcttt cagtgcttct cctttttctt cactacgtac tagtacatag atttcatttt
    38341 gtggaaccat cttttttaag aattccagcg cataaccacc aaaacctcca gttgctcctg
    38401 ttactaaata atgcatattt gccactccct attcttttta tttgttaaga tttacagctg
    38461 tacatttata aattacacct ttatcataaa gagtgtggta agataagtca agcaaaaaaa
    38521 atgaaaggat gacaactgat gaagtattct ctgcaattta gtgatgcaat ccatgtactt
    38581 gcctatattg aaatgtttca agatacccga tcactttcta gtgagatgat tgccagtagt
    38641 atcgaaacaa atgcagcaaa tgtacgtaaa atcatgagtc agctcaaaaa atccggtttg
    38701 atctttacaa aaaccggaaa accgcagcct tcccttgcca aaagtccaga tcagatttct
    38761 ttactagatg tctatcgcag catcgaaggt aatacacagc ttatccaagt cgatccaaaa
    38821 acaaatccaa attgcgtcgt tggtgcgaat atccaagaag ctttagaaga aagctacgct
    38881 cggctgcaga aaaaggcaga agaagaaatg gctaagatca cacttgatag tctgatacat
    38941 aaaatagccg ttttggaaaa agaaaaaaga ccagaaaaca caggcctcgt agagagattt
    39001 ttataattct ctctactgtg cctgcatttt tctgatcgga atcgtctaca taaattcttt
    39061 tattctccgg gggacaaaag ccttgtcatg cccctttttc ctctctaatg ctcaggtcaa
    39121 agcgctgaag tcatgacgtc ttgataaacg gtgttcaagt agctgaccct cggcattagt
    39181 tctgaaaaat gcaaaatatg gaaagctatt tcccattttt cctctctaat gctcaggtca
    39241 aagcgctact ttcacgacct ctatattatc atggtttatt gactaccggt atccagattt
    39301 ctcccatata tttcccgttg gtaactccca tttcgactac agcatttgga cccccaacat
    39361 aggcgtaatc ttctacttct cttaaaacat gcccaaaggc cgtattcgtc aaatcatttc
    39421 ttaattcttc aggcgattct gctaaagcgc ttacaacaag atacgttcct tttgggaatg
    39481 ggatgattcg cgtagtgttt ggaacatcct gactcgtttg aacaccgaca tagtacatca
    39541 ttttcccgtt atagggttcg ttgactgtaa agaaataatg attttcagca gcttgtttca
    39601 atgcatctat tgttccatta ccgataactt tttgctcaaa tgcttgtttc tctgcagcca
    39661 aaccgacaaa atccgtattg ctactcttca attccacacc aatacccgtc accgtaaaat
    39721 ctggtttttc ttcgatccta tactttgcca tttctcccac cctttctatt atattaagtt
    39781 catcttacat aaaattaaag ggaaatgaca cgattacgct cttttttcta gggatatttt
    39841 tattttttaa ctattttttt gctttcactg caatccaaat ctcactgtaa acattactga
    39901 aatcctctcc ccattttgta aaagagatct cgggagcttc gactaaccca taatctgaag
    39961 aaggaagcca ttctgcatag attttccccc aagtatcttg taaagtttgg ggaaatggcc
    40021 ctttattagg aaaaatggcc caagtgtgtt cttctatagc aagcatttct aaatcagcgt
    40081 acggattttc ttttgtcgta gcaaagccaa tcatgtgcat caaactgccc ttttcttcca
    40141 agcggtctcc atcaaatcca taagaaacgt tgattacttg atttggatat aaatctccta
    40201 acttatgcat ctcttccctt tgagtctccg tgatcgtctg cgctaacttt tgtatttctt
    40261 gattgactcc ttcaaatttg acaggaactt ttttcgccac acctgccaca taaaactgtt
    40321 ctttcttttc gatacgaaat tccatcgatt ttcctcctct gacatcaata aaaaatgtca
    40381 agcgtggaaa agatttttgt atgccgtatc tactgatttc tgaaggcaga tatcctgacc
    40441 aatcacgaaa agctcttgaa aaaccttcga ctgtttcata tccatatcta aacgccgttt
    40501 ctgtcactct tgatccatct agaagatcgc gattagccaa agccaatcta cggtttttga
    40561 tatagtccat tagcgacatc cctgcaataa acgtaaagat gcgttttaat tgatattcag
    40621 agattcctgt attttcggct attattttat aggaaagtcc ttctgtcaaa tgttcttcga
    40681 tgtaatcgat caattggttc agatctttaa gcatatttat cttcctcctt gattgccttt
    40741 ttatcatatt atcaaagaaa gtcaattact tgatgaatca ggaaccaatt tagtcagatg
    40801 tagaaaatgt tgtatggatc taaagaaaat tctcaagcag cgtttcgtaa cggataattg
    40861 gtattatctg cctatctatg ttttaccttt agaaaaataa attttgggga agacagattc
    40921 taagtaaaca aagcggtaaa aatatctttt attcccttag ttaatccgct tactacctac
    40981 tgtacaaagc aaattgttgt tcaaactgct gatcagaacg ctgcttggat ttttctttgc
    41041 gatacatcat acctctttta tcaattggaa caattattta tttctaacaa aatcacttca
    41101 tttttgtcag ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta
    41161 ctttttctca cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa
    41221 agtgattatc aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat
    41281 ataaataagt gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga
    41341 aaacgttttg gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta
    41401 tacaagcaag tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac
    41461 ttgggatgaa aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg
    41521 caaacacatg gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta
    41581 ctcactttcc gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct
    41641 tttaacagag attcttatag aacatccgca ctttatcgaa cgcttcaaag ttaagatgcc
    41701 atttcatgca ataaattatt cagataaatt aacaactatc atccataaat acgatcatat
    41761 tatcaagaaa aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat
    41821 ccaagaagca gttgaatcgc ttattcagca aaatgcctat cttcccgcat tcgaattgat
    41881 caatgaagtg attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct
    41941 tcttctgata gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc
    42001 agaaaaaagg caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta
    42061 ttcaaaacgc tactctataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa
    42121 ccaaaagaaa aaaatagata aaaaactaaa gaaacggtaa agaaagacga tttcaatgaa
    42181 aaatagcaag taaacgataa caaccatcct aaatccacaa gaaatacagc agaccttgct
    42241 ttaaaaacac gaatcatgca aaggatgaag taaagaagac ttaacgcgta cccaatatta
    42301 gtcatttgac tattaggtac gcgttttgtt ttataagtca tgacaaattt ccttcgttgc
    42361 tggatagatt ttccggcatt gttgatagat tttatgatac ttttctacat tttgtaagag
    42421 aggttccgct gcatctgtat attggataaa attctgacaa caactttcag gtgtgtcgaa
    42481 ccaccctaac cctagtgcag caatcattgt tgcgcctaat gcaggtcctt cctccatact
    42541 taaacaatag accgttgcat cgaaaatatc agcttggatt tgtagccatg caagattttt
    42601 cgcgcctcct cccaccgaaa caatacgtgt aaatctgcga cccgctagtt ctttacgttt
    42661 caatcccacc attgacaaat gctcattaaa aatttagaat attcacaagt ttaacgacaa
    42721 ttatgaaaat ttatgtcgtg ctaaaggagt tttgttattg gatgaaaagt tagatataaa
    42781 aactgcgatc aaagacacgt tacctaccgt tttcggttat atcggtattg gacttgcatt
    42841 tggtatcgtt gggaaagctg ccggatttca tccactagtc gtcacgttga tgtccctgct
    42901 ggtctatgct ggttctgccc aatttatcac agtcagcatg cttgctagtc acagcccatt
    42961 gctttccatc gttttctcga cctttctagt caattcccga atgattctga tgagtatgac
    43021 gattgctcct tatttcaaga aaaatcggct gcttcaaaat ctgctgattg gcacattgct
    43081 gacagatgaa agtttcgcac tgggaatgaa taaattgaac tatactggac aaaagttaaa
    43141 ctttcgttgg atgaatacag ctaattggat ttcttatttg acgtgggtgg gttcttctct
    43201 tgttggcgca cttttaggga attttattac tgatccgaaa aagtttggtt tggatttcgc
    43261 catcgtcgct atgtttatcg ggttacttta tcttcaagtc atttctgata gaaacacctc
    43321 taaacgatta cagttgatcc tgattggtct cactttgatt ctggtatatg tcggactgat
    43381 ttttatccca agcaacttag tgattgtagt tgtaacactg attgggtgcg gattgggggt
    43441 gtggatcaaa catgccttct tttgagttta cattgctgac tattttaggt tgtactttag
    43501 caacttggct ttctagggtc ctaccatttg tcttactgaa aaaattcgat ttaccgcaac
    43561 cgttgcttga gtatttaagt tttgtcccga tcgtcattat gtctgcttta tggttcagca
    43621 gtctatttac ccaaaatatc ggacatctgc cgcagatcaa tctcgaaaat gctttggctt
    43681 cagttcctac acttttagct gctattcttt caaaaagctt attggtgatc gtcttggcag
    43741 gaattctttc cctttcgttg attcgcttat tataaataaa aaaacaagag cttatggcaa
    43801 attactgtca tgcgctcttg tttttatcaa agtcaaataa gaaaaaaatc ggcgtattct
    43861 tgcttatccg ttgaacggac cactaacaac gacttttcct aacatatgtc ctgtttctac
    43921 gtctttggtt gcttgtttca agttcttcgc attcatccca tctgaataga ccttgctcaa
    43981 tgtactgcgg ataatccctt catttgctaa agcagcaatg tgcgctaaag cttctccttg
    44041 cgtttcgatt tcatagttat agtctgtttt agcgaacata tactcccagt cgaagctgat
    44101 agattggttt ttccaaacac ctatatctaa cggctctttg atccctacga tcgttcccac
    44161 atgtccaaat ggtcggacaa ggtgcttgat ttgatcaaag tagcgggtaa tatcgaataa
    44221 tacagcgaca tactctactt gatcgattcc taattcacga aaacttttac ttaagtcgtg
    44281 atgataatcg attggatagt ctacacctgt ttttttcagc cattcaaaat tcttcggact
    44341 tgcagtcgcg tagacttcta ggccagccca atgcgctagt tgattcaaaa tcgaaccgac
    44401 gcccccggat ccgttgatca ctaagatttt tttcccgcga ttggcatttt cttctggagt
    44461 tagaccaaat ttttcaaata acaattcata agcggtcaat gaggtcaacg gcaaggctgc
    44521 agcttcttca tctgaaagat tttttggtgc aagagcgaca atacgttcgt caactagttg
    44581 gtattcttga ttgcttcctg ctcttgttgt tgtacctgcg taaaaaacgc gatcgccgac
    44641 agaaaatttt tgcacttcgt cacctacagc aacaactttc ccgactccat caaaacctaa
    44701 gatacgtaaa gcatttctta tcccgttatc ttggcgaagt ttcgtatcga caggattaac
    44761 agatactgcg ttgactttta ccagaagatc ccgtgaactg ggtacaggta tctgttcttt
    44821 ttcatcaata aagctttttg gatcatcgat cggcaaccct tcataaaaac cgactacctt
    44881 cattaattcc ttttctgaca tacgaattcc tccttagctt catcaagttg gagcatccat
    44941 acagtgttca tttcaatata tatagtatgc tatttgttcc acctttttta ttcatcataa
    45001 ttattctctt acttaaagta aactatttgc acttttttgg ttaaaaaaga ggttgcgaca
    45061 agacttttgt cacaacctct tataaacggt gttctcctac caactcttcg gtcacaagtc
    45121 aaaatattca tcaaacatga gaagctgttt tttgaaattg ctccttgtga ctcagagtta
    45181 aacggcaggc tcacaacctc tgatataaac ggtgttcaat caactgaccc tcggtattag
    45241 ctcaaaaaac gacaaaatat gaagagctat ttttgtttgg ctgttcttat tacttgatac
    45301 aggacagctt tttcacgacc tctaattcac ggtgttcaag caagaaatca ttgttcactg
    45361 aaaagcgttg gatgttcttt ttcaaaggtc tctaagatct ctcgtacttc atcagtagat
    45421 ttcgtattca tcaactgaac tcgcaagtca tttgctcctg gaaatccttt gatatagatt
    45481 ttaaagaaac gatgtagtcc tgtgatggaa cgcgggatga tttctgaata atgatcttgc
    45541 agatctagtt gcagacgaag tagtccgatc agttcttttt ctgtatgttc cctcgggtct
    45601 ttttcaaatg cataaggatt cttgaaaatc cctcggccaa tcatgatgcc atcaacgcca
    45661 tatttctcgg ccaattcttc tcctgtctgg cgatcgggga tatctccatt gatcgtgatt
    45721 agtgtttgtg gagcgatttg gtcacgtaaa gcaatgattt taggaatgac ttcccaatgt
    45781 gcatctactt tactcatctc tttacgcgta cgcaaatgga cagaaagatt ggctatatct
    45841 tgatgaagta ggtgactgat ccattcttcc atctcagaca tttccgtata gccgatacgc
    45901 gtcttgacgc tgacaggcaa accgccagct ttagctgctt caatcaattg tgccgctact
    45961 tctggacgaa ggatcaagcc actgcctttt ccacgttctg ctacattagg gactgggcag
    46021 cccatgttga tatcgattcc ttggaagccc atttctgcca tagcaatgct catttctcta
    46081 aaaaattctg gcttgtctcc ccaaatatgg gcaaccatcg gctgttcatc ttctgtaaat
    46141 gttaatcttc ctcgtacact gtcttttcca tctggatgac agaaactgtc tgagtttgta
    46201 aactcagtaa agaagacatc cggtgctgct gcatgcttta cgacatggcg aaatacaaca
    46261 tctgttacat cttccattgg tgctaaaaca aaaaaaggct ttggcagctc tttccaaaaa
    46321 ttagtagtca tttatttgtt actctccatt ctatcaagca gtgaagtcgc aatctttctc
    46381 aaaaacattg tcattatact aggaaacaaa aaaagaagca aagtgaacag cttaaaagat
    46441 gtttctgatt gagcaaaaaa aagactgtga caaaaatctt atcacagtcc attttccgga
    46501 ataaatggtg ttcaaaagct tgcatttgcg gtaataagtc caaccaaacg aaaaatagga
    46561 ggagctattt ttgtttggct gttcttatta cttgatgcag aacagctttt tcacaacctc
    46621 tccactaaac ggtgttcaat cagctgaccc tcggtattcg ctcaaaaaac aacaaaatat
    46681 gaggaactat tttcccgttt gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    46741 tcttttctaa cttctcaatc ctcttccttt attgatcaaa taccaagtaa cggcgtacaa
    46801 aacaacgatg aataacacaa gtacgatcat cgatgttgct actggcacat cgatcgttcc
    46861 aaggaatccg taacggaaac cggagatcat ataaacgatc ggattgactt ttgaaacagc
    46921 ttgccaaaag ggcggtaaca tagagatggc atagaaaacg ccacctagat aagttaacgg
    46981 ctgcaagaca aatgtaggca cgatcgatac atcatcataa gactgagcaa aaatcccgtt
    47041 gagcagtcct gctaatgaaa acaagattgc cgtcatcaat aatgtaatga tgaccatgaa
    47101 ccatgaatac acatgaagtg gtacaaagaa caaggaaatg atcgttacga gagagccgac
    47161 gagaatactg cgacccacac caccaatcac aaagccccaa ataatcacat gcgtaggaac
    47221 aggtgctacc aagatttctt cgatattttt ctgaaatttt tgagagaaga aggacgaaga
    47281 aacattggca tacgaactag tgatagcaga catcataatc aaaccaggaa cgatgaattc
    47341 catatatgaa aaaccaccca tgtcaccgat ccgcccaccg atcatttttc cgaaaataac
    47401 aaaatacaag gatgtcgtga tcactggcgg aaccagtgtt tggacccata tacgcaaata
    47461 gcggttcgtt tccttgatag ctaaactttt taatgcagta aaataaagat taaacatttt
    47521 tctcctccgt atgattattt tcttctgtga ttttcaagaa cagttcttct aaacgattcg
    47581 acttattcct catagaaaga actttgattc cttgtcttgt aagctgatcg aagattccgt
    47641 tcaccccttg atttcgttca acttcgacag cgattgtctg ctcatcttca aatgtacaag
    47701 catatccttc aatcacaggt ttggtatcat atggcgctaa atcaaagata aatgtttcaa
    47761 attgcagttt tgacagaagc tctttcatac ttgtattctc gatcagttca cccgattgga
    47821 tgattccgat gttgcgacaa agcatttcag cttcttctag ataatgcgtc gtcaaaatga
    47881 tcgttgtacc attttcgttc aattctcgta gaaactccca catctcgcgt ctcaactcga
    47941 tgtcaactcc tgctgttggt tcatccaaga tcagcaaacg tggttcatgc atcaatgccc
    48001 tagcaatcat cagtcttcgc ttcattcccc cggaaagcat acgtgcacgg acatttctct
    48061 tttcccaaag attggactgc ttcaaatatt tttcgctgcg tttcatcgct tctttgcgag
    48121 agacaccgta gtagcctgct tgattcacga cgatttgctg gaccgtttca aaagggttaa
    48181 aattgaactc ttgtggaacc aatccaatct gttgctttgc tcgaactaga tccttgtcca
    48241 gatcataacc gaagacattc acttttccag atgttttatt gactaaagaa gtaataatcc
    48301 cgatcgttgt agacttccca gctccatttg gccctaatag cgcatagaaa tctccttctt
    48361 tcaccgaaag atcgattccc cgcaatgctt cgaccccact tccatagatc tttttcaaat
    48421 tatttatttc aagcgcattt gccatttatt tcctatctcc ttatagacat gattcttaat
    48481 tgtatcatgg aatacagcag atgaaaaaac aacttggctt ccacacaaaa acacacctgc
    48541 taacaaagag gagatgtgtt ttctagttca tgatcatctt cgatagaata actatctaaa
    48601 caactctgga acttttaaaa tcataaaagt accgacagca actactacga tgacatatag
    48661 aagatcctta taactgaggt tcttgtcttt tttattcatg ttaagctttc ctccgtcctt
    48721 ttatcgaaag cttttttctt ttattttaac cgaaataaat aaaatggaaa tagttatttt
    48781 caatttctgt tttcgctgtt tgttttacgg agaactttgc catggaacgt gcgtctcgtc
    48841 gctgatgcta agttttttcg ataaaagctg ctagaaggaa gacagcgtgg tttattttgt
    48901 atcgtctttt gctaaatctt ccaagatcat ctggacagca gactcgttcc cattgaattc
    48961 tgccactttt ttggcatcaa tgtacttctt ttgataatcg tctttttgga cccacgcatc
    49021 atgcttcgtt tgataaagtt aatcaaacgg ataccactga cgctgttcat cgaacaacaa
    49081 actttctttt tgggcctgtt caactatgga tttaggataa ttcaaaactt tcagcaaggc
    49141 aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac tgattccttt
    49201 tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta aaatctctgt
    49261 caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa gccaccgaac
    49321 gactgaagcg gaagaagcaa tccgttcgat cgtatttgtt ccttttaaaa tctcatctac
    49381 gaagcagtag cagcgttctt tggaagccac ttggtccagc aaacgtttga tcgatttgat
    49441 ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca tggaagtcaa
    49501 cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg tctgtgctag
    49561 aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat tggaacccgt
    49621 gactaatgta ttcttctgcc agttgactgg attaactacc gcttctttta acaatggatg
    49681 atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag tgatcggcat
    49741 ataggtgcga taattcaaaa cggctgctgc aacttccaat tctcccagca attcccacag
    49801 cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat aggagataaa
    49861 tggcagcatg aacatgatgt tgacatattc aaaaatcaat tctgcttcac tcccgctctt
    49921 cattcgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt gctgtttcag
    49981 ctctttttga agtggtgtcg tcatttttgc taactgattg gcggctgaaa tcgttgaaac
    50041 caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta gataatagac
    50101 cgtattgaat actaagccac cgattgctag atagattcct agtacttgcc ctaaaagaag
    50161 caaacctaca cccaccagcg gaagcaagcc aagaaaaata aataaaccta cgcgtccaat
    50221 ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt tttttcctaa
    50281 acgagcgaat tgatattgga ttctttctct cagttgagga ttttcctgat aaaaagcaat
    50341 tagtttttct aactgataat cttctccaaa atcaaagcta cgtaaacgct gatacaacgc
    50401 ttctgaacct acactagaat aggtactgtt gatcccttca aatacttcga acatatccaa
    50461 gtcataccaa gtaagatcgt cgatttcact atcccatgaa cggaattttt tctccgtgag
    50521 ccacgcatcc ttcaaacttt cttctttatc aaatctcggt tgataaggaa tttttcccca
    50581 attactacga actgtttcct ttactttcaa acgtgaaaaa atgtctaaaa caactaataa
    50641 aataacaact gcaataataa ttaaaacgat aaattctctt tggcccatat ccctctccgt
    50701 tccttgattt gttagtttta gtatagcact gttgcgctat ttgaaaaggg ggtacaaaaa
    50761 taactgtctt cgttacctaa ttcgtatcaa tcacaccaaa ataaaaacac cttccaaaaa
    50821 agaagatgtc tttaaaacga gatacaattt tttgtcattc acttcaggta aattagagcc
    50881 aattaaaccc tccgcaactt gacggagatg gagaagatgc ccccgtctaa tctagcggtc
    50941 atctctcctc ccattccttc caccaatttt tttgacaggt aaagcccgat tccgctagat
    51001 tccgtatttg tgcggctggt attttcggta taaaaacgct ggaagacttt ttcaacagag
    51061 gtttgcgctc ctggtttgat aggattggcc actgttaatc gaatgaatga atcctctttt
    51121 gaaaatgtca cctttgcttg tccatctgca taacgcaata aattctgtat gatattttgc
    51181 agcactcgca gcaacatgag cggatctgct aatacagttg tatttatcga gtgttcagcg
    51241 aacgctacct cgatattttt ctcagaaaat tgttcataaa aagagaaaaa tacttcttcc
    51301 acctctttag aaaaagaaag cgtatggaga tccatttctt tttgcttcgt ctctattcga
    51361 gcaagatcat aaaactgatc cgtcatactg agtaatcttg caacagattg tttgatgatc
    51421 gttaaatact tctcccgttt ctccgtatcc tctacctgtt ccagtaattg tacataaccg
    51481 ttaatagcag tcaaaggagt tctgagatca tgagaaaggc cgacgatcga tagtctgatg
    51541 ttttcttcca tttcttgaaa tgcttgtcct ttttgttcat tttcatccac ccatgtattc
    51601 agtgcatcta ctaggtccat tagattcttt tctcgaaatt cgactgaaag acgaccacca
    51661 tattcttgtc gttcagaaag agtggcgatt ttcttagtta atctcttgat ttccctttgc
    51721 aggaagaaaa taaaacttcc cataaagata acaacgagaa gcaacagcca cttaatcaaa
    51781 tctaaacccc actccccaca ccgtatgaat atacacagca tgtgcctttg ctagtttccc
    51841 tcgaagattg gaaatatgca tattcactgt tttctcacta tcaaaatatg gttcttgcca
    51901 aactgtttca taaagatttg ctcgcgaaaa tacttttttc ggatgttcta aaaaaagctt
    51961 cagcagatcg aattctcttc ctgtcaaatg gacagccaca ccattcacat ttacatccct
    52021 cgactctttg tccagtaaga tttccttgta atagatgcgt tgcgacagat cttttacttg
    52081 gacagaagtc tgatgacgaa gttggatgcc gattcgtaac aacagttctt cgatatcaaa
    52141 tggtttcgtc acatagtcat ccgctcctgc tgccaacaag gaaactttat ctctttgatc
    52201 gctttttgct gaaagaacga tgatcggaac attcgatgtc tgtcggattt ctgctaacaa
    52261 tgcttcacct gaaagccctg gaagcatgag atctagaagg atcaaatcga caggatatgt
    52321 gcttaacaat aattttcctt ccgtacctga ataagcatca agaagtttgt atctctgttc
    52381 aagaacctcc tccagtaaag aatgaacagc ttcatcgtct tcgataatca aaatagtttc
    52441 cagcaggaac acctacttaa tgtcagattt ttggaaaata aacattccta gagctgatga
    52501 taccacgatt gttactagac tgaccagaat cattttcggt gcttgcgaat aagttgcttg
    52561 ttgcgcaaag tccgtataca tgagccacgt ttccgtaaac cactccgtta gaaattccaa
    52621 gcctttgatt tttgcgaaaa caccgggaac gaaggccgtt cccatgatat aaagaaaaga
    52681 aagaagcaaa ctgacactgg tacttctagt gatgaatgtc aaagccactg gaacagataa
    52741 tgtcgctaat atcagcagaa gcaatacagg aaatactttt agaacataat ttccatagtt
    52801 tttgattccc tcaacttcac caaatccgaa agcaagtgtg taagcaatca tggctagccc
    52861 cgtaatcaaa accacgccaa gtgttgtgat caaaagcgta acgagaagtt tcgacacata
    52921 ataagtcatt cgattccgtc ctaaagataa cgtattgcga atcgtaccgt tttgaaattc
    52981 ttccccaata taaaagcacg caaatgctgc gaaagccaga agaatccatg cagccatttt
    53041 ttccaccatt aaaatccctg ttgcaccgtt gatgactgta tccgttgctt ggtttaattc
    53101 attgccgtat tttgtatgca tataccattc gttccaagaa taaaaaagcg gaagtacgac
    53161 catcagtcct agtgagaccc acattttacg gctcttccat acttttattt tttcagcagt
    53221 taatagatta aacatgtgga ttgcctccag ttaattgtaa gaaataatct tccagttttt
    53281 gcccaactag gtggatacca tccacaggaa cttttgcctc taccaatgct tgattgatgg
    53341 ctgctacttg gtcaattcct tcaaaaagct ggataacacg gctagattgg acaaaataat
    53401 ccctataacc aagagagtcg agaaccgtta ctgcttgttc cgtcgccgaa gtgtcaattt
    53461 ttataaattg acgagattct tgtgccaatt cttctttcga taattctttg atcaagtgtc
    53521 cttcatgcaa aataccgtag tgagtggcga tctgtgacaa ttcatctagc aagtggctag
    53581 aaagcaagac ggtgatgccc cgttctttta ccaaacgttg gatgatttct cgcatttctg
    53641 ccatccctgc tggatccatc ccattcgtcg gttcatccaa aatcaaaaat tctggattcg
    53701 tgatcagtgc atttgcaata gctaaacgct ggcgcatacc taaagagaaa ttctttgctt
    53761 tctttttccc tgtattttcc aatcgcatca ttttcagcag gtcttcgatt tctctatccg
    53821 acacaccgcc gttcgctgct tggatacgta ggttatctct tgcagttaat tctggataaa
    53881 gagcaggggt ttcaatcgat tgacccatac gtcgtcttgc tgcctgcaaa tccgtcgttc
    53941 caaataattc gatcgaacct tcatcaatcg agatcaaacc cataatagtt ctcataaagg
    54001 tagacttacc cgctccattc tccccaatca aaccatagat cattccacgt ttgatttcga
    54061 tcgatacttt atctaatgct ttggcagcac cgtatttttt ggttatacca gttgctttca
    54121 agaccgtttc atttccaaaa tccatactta tccttctttc ttattgataa gtgaagtata
    54181 gttgacgggt ataaagaaag cagccagaaa tgtataaaga aagtgtaaat tttccaattg
    54241 aatttcaaga aaaatcagac tgtctcgcag acgtggttga gttgtctgcc aatcaaagag
    54301 cgcaccaaac agtgctaatc caagtggact ggccaccatg gtaatcgttg ttatcgttga
    54361 catcagacgt ccttgtatat gcgttggaac ttctcgctga agccagacca tataaaaagc
    54421 aaaagaaaac attaatcctt aaaaacggac ttatgaaaaa taatacatgc cgtttgggat
    54481 ttacaattag ctaccaaata agaggttatg acaaaagact tttcacaatc ccttcttccg
    54541 aataaacggt gttctcctgc caactcctcg gtcacaagtc acaatattta tcaaacatga
    54601 gaagcaattt ttgtaaattc cttcttattt cttgaagctg accacttctg tcacaacctc
    54661 agaagcggat aaataatatt gtttgatgat actttatctt tttcccgttt gtttatagat
    54721 cgaatgatat tatagaccaa gaatcctacc tattaacata gctaaaaaca caaaaaaggc
    54781 actttccaaa ttgtggaaag tgccagaaaa gttaggatta aggtaactgc gctcagcatt
    54841 gcttcgccca gtacgattct cgtaactcgt gaactcgaac gataatcgca attaacgttt
    54901 tgagaactgt gaagctttac gggctttctt aagacctggt taaacaaaac tctattttat
    54961 tgcattttac aggaatgtat ttcatgcgaa attaataagt ctttgtttat agagttttac
    55021 tagggtataa aaagttgtat gttatcatta cctcaaaaca ttacctcagc ttggcgagtt
    55081 ggttgattga agaataaatc atttatctag atatctcaat aaaaaatgat ttctaagaaa
    55141 cttgtatttt cgtgttatta tttttttgcc ctatcaaaat ttctaattct tctagcgtaa
    55201 tatgattatg acacataatc gattcaatta cttcaatacc tatttttttc gagaaagatc
    55261 ccttttgttc actcacaaaa atactttcag aagacatata taaggaacga agttttggaa
    55321 ttagtatatc gtacaaatag accaccataa aatcgtcctt gcaaccattc tcaaaaagaa
    55381 ttaaatttgc tgattgtgtt acatatatca ctgaattaca ttcatggtca attccaaaaa
    55441 caatgtctac ttcattttct tttctaattc gcgcagttac atttttcaaa ctttcaccat
    55501 tacttataaa tgctttagtt actgtaagct ctgatgtttg ttcagatatt ccttttataa
    55561 tattttgttc atcagccaca ttccccataa atccgccgat tccttttaag taataattct
    55621 cacctaagag tcgcttgcat tcactgaatt gataaaaaaa ccagttaaaa aaatcttctg
    55681 gaaaaataaa ttctgatcga actttattta tatttttagc tcctattaat tttataactc
    55741 gattaatgtt cacttgctgc gatgaacata taactgaata aattgagttg tttaattcaa
    55801 aaaaacaact ttaacatatg catcattaat tcgttatttt ttaggtagat attgatcatt
    55861 gttatctctc tttttttgct tcccatactc caacttagaa attgccgtta taaaatttat
    55921 tttaaaggtg actttttccc ctgaagtatc ggtagtagga atacttaata cctcagcttc
    55981 tgaggaggta tcccaattat agttatactt tgcatcatca ccataaagaa caattccatt
    56041 ttttttatct tgtgccgtta gctttactgg ttcgcttact tttcttgtta atatcttgga
    56101 ctttacatca tcgatattgc ttattttatc aagcttaaat aatttacaaa ttaatttcgt
    56161 actagttttt atatttg
//
LOCUS       DANKEF010000015        55807 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000015 DANKEF010000000
VERSION     DANKEF010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55807
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.015"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            137..994
                     /locus_tag="PWZ48_000997"
     CDS             137..994
                     /locus_tag="PWZ48_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDL2260669.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="PWZ48_000998"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="PWZ48_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HDL2260670.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="PWZ48_000999"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="PWZ48_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDL2260671.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="PWZ48_001000"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="PWZ48_001000"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2260672.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /locus_tag="PWZ48_001001"
     CDS             3750..4508
                     /locus_tag="PWZ48_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HDL2260673.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="PWZ48_001002"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="PWZ48_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDL2260674.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..5735
                     /locus_tag="PWZ48_001003"
     CDS             5436..5735
                     /locus_tag="PWZ48_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260675.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKK"
     gene            5761..6468
                     /locus_tag="PWZ48_001004"
     CDS             5761..6468
                     /locus_tag="PWZ48_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2260676.1"
                     /translation="MSIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLL
                     NISILFVVIGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYE
                     ELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQV
                     GRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVL
                     SIPISVLFFLTVRRLKR"
     gene            6465..7232
                     /locus_tag="PWZ48_001005"
     CDS             6465..7232
                     /locus_tag="PWZ48_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HDL2260677.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7332..8900
                     /gene="liaX"
                     /locus_tag="PWZ48_001006"
     CDS             7332..8900
                     /gene="liaX"
                     /locus_tag="PWZ48_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HDL2260678.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9197..9517
                     /locus_tag="PWZ48_001007"
     CDS             9197..9517
                     /locus_tag="PWZ48_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HDL2260679.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9517..9867
                     /locus_tag="PWZ48_001008"
     CDS             9517..9867
                     /locus_tag="PWZ48_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2260680.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10034..10972
                     /locus_tag="PWZ48_001009"
     CDS             10034..10972
                     /locus_tag="PWZ48_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HDL2260681.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10987..11817
                     /locus_tag="PWZ48_001010"
     CDS             10987..11817
                     /locus_tag="PWZ48_001010"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2260682.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11861..12886
                     /locus_tag="PWZ48_001011"
     CDS             11861..12886
                     /locus_tag="PWZ48_001011"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDL2260683.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12901..13839
                     /gene="galU"
                     /locus_tag="PWZ48_001012"
     CDS             12901..13839
                     /gene="galU"
                     /locus_tag="PWZ48_001012"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDL2260684.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13964..14404
                     /locus_tag="PWZ48_001013"
     CDS             13964..14404
                     /locus_tag="PWZ48_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HDL2260685.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14407..15009
                     /locus_tag="PWZ48_001014"
     CDS             14407..15009
                     /locus_tag="PWZ48_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2260686.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15077..16183)
                     /locus_tag="PWZ48_001015"
     CDS             complement(15077..16183)
                     /locus_tag="PWZ48_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HDL2260687.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16393..17394
                     /gene="ccpA"
                     /locus_tag="PWZ48_001016"
     CDS             16393..17394
                     /gene="ccpA"
                     /locus_tag="PWZ48_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HDL2260688.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17730..20075)
                     /locus_tag="PWZ48_001017"
     CDS             complement(17730..20075)
                     /locus_tag="PWZ48_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2260689.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20354..21256
                     /locus_tag="PWZ48_001018"
     CDS             20354..21256
                     /locus_tag="PWZ48_001018"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDL2260690.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21469..23244
                     /locus_tag="PWZ48_001019"
     CDS             21469..23244
                     /locus_tag="PWZ48_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HDL2260691.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23501..24718
                     /locus_tag="PWZ48_001020"
     CDS             23501..24718
                     /locus_tag="PWZ48_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2260692.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     AHRLAQTHLKNQRQPKEVQSNFYLEMRDLARFYQEIEKKITRLRMDLHNCLQLTFPEL
                     EQFFLNRLTPYALILIRLFPHPDFVLASTRTKIKNKLINETRKKISANRAEQKADQII
                     HYAQCAYPAVEKDSIHCQKTIYYAELLQDLLEQKEALATQMIKKAEGSPCFLLYQTFP
                     GIGALTAALLLGELGDITRFKTHKQLNAFVGIDIRRYHSGKYTGQDRINKRGNPKARK
                     IIFFTIRNMIRQQRAAPNHIVDYYYKLKKQPIPKKDKVATVACMNKLLKCMHAMVRAH
                     TEYDYAYAVSVDH"
     gene            complement(25165..26751)
                     /locus_tag="PWZ48_001021"
     CDS             complement(25165..26751)
                     /locus_tag="PWZ48_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDL2260693.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
     gene            27002..27226
                     /locus_tag="PWZ48_001022"
     CDS             27002..27226
                     /locus_tag="PWZ48_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HDL2260694.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            27368..29119
                     /locus_tag="PWZ48_001023"
     CDS             27368..29119
                     /locus_tag="PWZ48_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260695.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            29119..30903
                     /locus_tag="PWZ48_001024"
     CDS             29119..30903
                     /locus_tag="PWZ48_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260696.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            31361..32212
                     /locus_tag="PWZ48_001025"
     CDS             31361..32212
                     /locus_tag="PWZ48_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2260697.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            32309..34195
                     /locus_tag="PWZ48_001026"
     CDS             32309..34195
                     /locus_tag="PWZ48_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2260698.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(34236..35138)
                     /locus_tag="PWZ48_001027"
     CDS             complement(34236..35138)
                     /locus_tag="PWZ48_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2260699.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            35368..36771
                     /locus_tag="PWZ48_001028"
     CDS             35368..36771
                     /locus_tag="PWZ48_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2260700.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            36768..38204
                     /locus_tag="PWZ48_001029"
     CDS             36768..38204
                     /locus_tag="PWZ48_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDL2260701.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            38349..39275
                     /gene="trxB"
                     /locus_tag="PWZ48_001030"
     CDS             38349..39275
                     /gene="trxB"
                     /locus_tag="PWZ48_001030"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDL2260702.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            39488..41215
                     /locus_tag="PWZ48_001031"
     CDS             39488..41215
                     /locus_tag="PWZ48_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HDL2260703.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            41231..41581
                     /locus_tag="PWZ48_001032"
     CDS             41231..41581
                     /locus_tag="PWZ48_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2260704.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(41665..42525)
                     /locus_tag="PWZ48_001033"
     CDS             complement(41665..42525)
                     /locus_tag="PWZ48_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HDL2260705.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            42617..43045
                     /locus_tag="PWZ48_001034"
     CDS             42617..43045
                     /locus_tag="PWZ48_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2260706.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            43207..43383
                     /locus_tag="PWZ48_001035"
     CDS             43207..43383
                     /locus_tag="PWZ48_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDL2260707.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            43410..43856
                     /locus_tag="PWZ48_001036"
     CDS             43410..43856
                     /locus_tag="PWZ48_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HDL2260708.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(44012..45391)
                     /gene="brnQ"
                     /locus_tag="PWZ48_001037"
     CDS             complement(44012..45391)
                     /gene="brnQ"
                     /locus_tag="PWZ48_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2260709.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            46097..47068
                     /locus_tag="PWZ48_001038"
     CDS             46097..47068
                     /locus_tag="PWZ48_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDL2260710.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            47092..49284
                     /locus_tag="PWZ48_001039"
     CDS             47092..49284
                     /locus_tag="PWZ48_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HDL2260711.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            49301..49777
                     /gene="ybeY"
                     /locus_tag="PWZ48_001040"
     CDS             49301..49777
                     /gene="ybeY"
                     /locus_tag="PWZ48_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDL2260712.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            49761..50156
                     /locus_tag="PWZ48_001041"
     CDS             49761..50156
                     /locus_tag="PWZ48_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HDL2260713.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            50171..51070
                     /gene="era"
                     /locus_tag="PWZ48_001042"
     CDS             50171..51070
                     /gene="era"
                     /locus_tag="PWZ48_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDL2260714.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            51213..52025
                     /gene="recO"
                     /locus_tag="PWZ48_001043"
     CDS             51213..52025
                     /gene="recO"
                     /locus_tag="PWZ48_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDL2260715.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            52409..53326
                     /gene="glyQ"
                     /locus_tag="PWZ48_001044"
     CDS             52409..53326
                     /gene="glyQ"
                     /locus_tag="PWZ48_001044"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDL2260716.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            53328..55403
                     /locus_tag="PWZ48_001045"
     CDS             53328..55403
                     /locus_tag="PWZ48_001045"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDL2260717.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        18079 a         9980 c        11845 g        15903 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa aatgatggct ggcttttctt cttcacaagt
     5761 atctccattg gcgccatgtt gcttattggc ggaaaacagg gtctatttgc tttaggaagt
     5821 ctcatcatca atatcgtttt acttttactt ttgttgctca tctttactaa aacagaaggg
     5881 atttctctat taaatatatc tatcctgttc gtcgttatag ggatgattat tagcatattg
     5941 gctttagatg gatggaatag aagcagtata atcaaaattt cagctgcagt tacgagtgtt
     6001 tttttagcat tttttatttg tcttttgaca atgaatcatt ggaaagacca aggactgcgc
     6061 tatgaggaac tgaattatct gacacgaccg tatcgttctg tttttctttc tagcgtactg
     6121 attggaacag ctggaggaac actcgatacc gttatcacag tcattgcgac attagaagaa
     6181 ttgactaagc gacaaccaga aataacagcg aaacagttat ggctttcagg aaggcaagtg
     6241 ggtcgagatg tgattggctc gatgaccaat atccttttgt tcgtctatat cagtgggtca
     6301 gttccatcgc ttcttttata tttaggaaat caatggtcgt tcaaagaaac gatagaacag
     6361 catttatcat tggaattttt acgcgtcatt gctggcagct taggaatagt attatcgatc
     6421 cctatctcgg tcctcttttt tctgactgta aggaggctga aacgatgaat gtacttatta
     6481 tacttgttgg gattttagca atcagcgtgc tacttattgg cggtacgcaa ggtatgtata
     6541 ttcttttagg cttattcatc aatttaggga tctttttcct gttgttattc ggttatcatc
     6601 agaagtggcc gatactggca ttgtcggtca taggattttt attgattgct gtggttatct
     6661 tattttttat caacggttat aatctaaaaa tgcgtgcagc ttttgtgagt atactgatct
     6721 ttttattttg tttcctacta ttgcttccta tcactgattt tttagcgatc caaggtttta
     6781 catcggtaga actagaagaa ttatctggct tagataagac tttagcgatt gattttagat
     6841 tacttacgag atcgttgctt ctgattagtc ttagtggtgc tgttttagat gctagtgtag
     6901 caatttctag tgggacattc gaagtatatc aggcaaatcc gcaattatct ttttaccaat
     6961 tgcgccattc tagtttcgct attgctaaaa aaatattggc gtcaacagta atgacacttc
     7021 tttttgcatt catgggaagt tctttagcgt tgatcctctg gtttattgat ttagacattc
     7081 cttttcagca agtgatcaat gaaaaatcat ttgttctaga atatacgatg gctattctta
     7141 ccacattgtc tgcactactt gttcttcctt taacctgcgt gactacagca tatctcttta
     7201 caaaaaagaa agaacaactc aagatggagt aaaagttaca acttaagtcc tatgacattt
     7261 ttactgaatc agtgcataat atggatgtaa ggaaaagctt gcaaacaatt ttgcttaagg
     7321 aggcaatttc catgaaagaa agagaacgta ttttagattt agtgaaaaaa ggaatccttt
     7381 cgactgaaga aggcttagat ttattagaaa gcatggcaac tgaaaaagac gaaaagcaaa
     7441 taaaaaaaga agcagacaag gtaactgcta gtcataaaga aaaagatcag gcaagtcaat
     7501 tgatcgataa acttgaaaac ggcgaagaag aaatttctga gccagttgat ccaaaggaaa
     7561 gagaaaggca agatcaagaa aacttagaaa agatcttaga tgaactagca actgaagcga
     7621 ataaaacttc ggcgcgtcta gatgaagtaa atgctcaatt cgctgacaat aaggcagcac
     7681 gcaatgagaa acaggaagca ttgatgcagc tgaatacaaa agaagaatta ggcgagttga
     7741 cagaagaaga attagcacag cgtcagacgt tagaagcaga aatcaaagaa ttagaagcta
     7801 gtggcgatac actgttagaa gaacaattga aattagaagc agaattaaaa gatatccgta
     7861 agaatcaatg gtctgagaaa aaagaaacgt ttactgataa gtttgaattg ccagatgatt
     7921 ggaaagatca agcaacagat acactgaatc aagtaggcga aaaaatgtca gaagctggat
     7981 cacagttagg caaattcttg aagaaaacat tccaaactgt ttcagaaaca gtgaatgaca
     8041 acatggaatg gaaagatgtc agcttacgtg tccctgggat tgcgacaacg aaatttgagc
     8101 atgaatttta ctatgaagct cctgcagcaa gtatccttga tatcaaggca gcaaacggaa
     8161 acgtgacgtt aaaaacgtgg gacagcaacg atgtgaaagt tgaagctaag atcaaacttt
     8221 acggaaaaat gggtgctgaa ccatttgaag cattctctga acggagccag attgaggtta
     8281 atgaagatca tatctccttc caaattccaa ataaacgtgt gcgtgcagac cttgttttct
     8341 acttacctaa acgagtatac gaccacgcag ctattaaact tctaaatggc aacatcatga
     8401 ttgaaacatt ggaagctaaa gatatttata caaaatctac gaatggtaac atcatcgtgg
     8461 accaattaac tgcaacgatg ctggaagtag aaggtgtaaa cggcaatatc gatattcgaa
     8521 acgggaacat cttagattct attatcgaaa cagtaaatgg tacagtgaca tttggtgcga
     8581 cagcagaaaa cctttctgtg tctttggtaa atggtgatgt acgtcttacg attaaagaag
     8641 ataatttgaa aaaagtagaa gcaagttcag tgaacggaaa tgtcaaagtc gctttaccag
     8701 atggcatcgg tttggaagga catgctaaaa caagtcttgg aagtatcaac agccgtctgt
     8761 ccaattacga agtagttcgt gagaaaaaag aacggacaaa ccaaatgttg caattccgtc
     8821 gtgtttcaga tggtgaaatc acgcaagtgc aactttcaac gacaacagga agtatctatt
     8881 taaaagatac agataaataa agaggttgtg aaagagatgt tcagccttga gcaataagaa
     8941 gcgattttgt aaaatagctt cccctatttt acaaaattgt gccttattgc cgataggcta
     9001 tctcttgaac accgtttacc aagagattat gaacctgcca attatcgccg agtcacaagg
     9061 aataatttca gaaaaagctc gtcacgtttt atgaatatgg taatttgtga ccgagaattt
     9121 tgcagaaaga caccgattaa gaagaaggtt gtcacaacga ttttcttcat ttaaaatagt
     9181 agaacagagg tgagacatga tgaaaaaaaa gttgacaaaa tccaataaaa atgttgtttt
     9241 gacagggact ttagcaggaa taggagaata catcgggatt gatccgactg ttatccgtgt
     9301 gctttatgta ttcctgagct tagttgcttt tggaagtcca atcattttat acattttatt
     9361 agctttgatt atcccatctg cgcgttctgc acgaacaaac tatggacata acaatgcata
     9421 ttatgaaaaa aacaattatc gtgaacaaac tcgtgaacaa acaaaaaaac gcaaagaagc
     9481 ggaaaaaatc gatgatgacg aatggagtga cttttaatgt cgtactttca acgtatttta
     9541 gttaatacgt taacattcat atcattggct gttctttttc ctgcgatggt ccatgtgagc
     9601 agtatttgga cggccgttct tgccagcttc gtattgtctg tgttgaatat gtttgtcaaa
     9661 ccgattttga cgatactgtc cctgcccttt acattagtca cattgggatt gtttacgttt
     9721 gttatcaatg cagcgattct taaaatgacc tccttttttg tgggagcaga acattttagt
     9781 ttttcttcat tcggtgcagc gttgttagta gctatcttga tgtcgcttgt caatgtaatt
     9841 gtcagtgaac ataacagtag aaaataaaag aaagctgtgc cgaactatcg gcgcagtttt
     9901 tttatttaga atcaggaatg cgttttctat tacattggta attaaataaa aacgtttttt
     9961 ttaattggta gtacttttgg ctagacaatg ataaaatgat agagaacact ctaatagaaa
    10021 tgaggtagca tgaatgtcag aagttgtaaa agttagccaa ttagtgaaaa aattatcttt
    10081 ggaaatcgtg acaggcgacg agaaaagctt agaccgtgtc atcgtaactg gggacatctc
    10141 acgcccagga ctagagttga ctggttattt taattattat tcccataatc gtttacagct
    10201 atttggcagt aaagaaatca gttttgcgga acggatgatc ccagaagagc gtttgatcat
    10261 catgagaaga ttatgtagtg aagatatgcc cgcatttatt atttcaagag gattaccggc
    10321 tccagaagaa atgatccagg cagcaaatga aaatgggata gctgtactgc gttctcccat
    10381 ctcaacttct cgtttattag gagaattatc cagttattta gatggtcgcc ttgcccctcg
    10441 aacaagtgtc cacggtgttt tagtggatgt ttacggatta ggtgtactga ttcaaggtga
    10501 cagtgggata ggaaaaagtg aaacagcgtt ggagttgatc aaacgcggtc atcgattgat
    10561 cgcagacgat cgagtagatg tttatcagca agatgaatta acggtgatcg gagaaccgcc
    10621 taaaatcctg gaacatctaa ttgaaatccg aggaatcggg atcattgatg taatgaatct
    10681 ttttggtgcg agtgcggtac gtggctctat gcaagtccag ttggctgttt atctggaagc
    10741 atgggcaaaa gataagaaat acgaccgtct gggctctgaa gacacgaccg ttgaaatcgc
    10801 ggaagtgggc attccacaaa taaaaatccc tgtcaaaaca ggacgaaacg ttgcaatcat
    10861 cattgaagta gccgcgatga atttccgtgc acgtacaatg ggctttgacg caacgaaaac
    10921 atttgaagaa agattaagtc gcttgattga agaaaactca ggcaacgact agcaaggagt
    10981 gacaatatgt tagcacaaat caatcgagtt gcttttgagc ttgtagggat tcctatctac
    11041 tggtatgcat tgatcatcgt atcaggtatc attatcgcca tgtggcttag cagcagagag
    11101 gctgttcggg taggattaaa agcagatgat gtgacagatt ttatgttgtg gggattgcct
    11161 atctccatta ttggtgctcg cttatattat gttttatttg atcttcccca gtatatagct
    11221 gatccaatcc aaatattcaa tatccgttca ggaggcttag ctatttatgg cggactgatt
    11281 gcaggcgcga tcacgctgta tttttttact aaatatcatt ttatttcttt gtggactttt
    11341 ttagatatcg ctgcaccaag tgtattatta gcacaggcaa tcggtcgttg gggaaatttc
    11401 atgaaccacg aagcatatgg accagacacg acgcgaagct tcttagaaag tctgcattta
    11461 ccgcaattca tcattgataa tatgtatatc gaagggatct atcgtcaacc aacatttctt
    11521 tatgaatcac tttggagttt gattggattt attttgcttt tgtttttacg aaagaaacca
    11581 caccttttga aaaaaggcga agtcctttta tgttatctaa tgtggtatag tttcgggcgt
    11641 ttctttattg aaggattacg tacagacagt ctttactttt taggtgtgat ccgtatttct
    11701 caattattat ctgctgtgtt atttgtcggt acattgattc tatttatttg gcgcagaaaa
    11761 aaaggaaatt taccggatta tgaccgctca ttaggaaaaa atcaacaaat tatctgattt
    11821 ttagagcgtt aaaaatgaaa aaaatttcag gagtggtaaa atgaattata gagtagcggt
    11881 tttaggacca ggctcttggg gtactgcttt ggcacaagtc ctcgccgaaa acggacatga
    11941 cgttcggatt tggggacatc gtgcttcaca agtagaagaa atcaacaccc aacacacgaa
    12001 tcaacgttat ttaccagatc attatctacc cgattccatc caagcattca ccaatatgga
    12061 agatgcagta aaagacgcag atgcggtgct attcgtcatt ccaacgaaag caatacgttc
    12121 tgttgcaaaa gaactagtgc ctaaattgaa tacaaagcca gtgatcatcc atgcaagtaa
    12181 aggattagaa cagggaacac ataaaaggat ctctgacatc ttgttagagg aaatcccagc
    12241 agaaaaaaga aaagctgttg ttgttctttc tggaccaagc catgcagaag aagtagcgaa
    12301 acatgacatc actacgatca cagctgcaag tacagatgca aaagcagcaa aatatgtaca
    12361 agagctgttc atgaataatt attttcggat ctataccaat cctgatgtga tcggtgtaga
    12421 gatgggggca gctttgaaaa atattattgc tattggtgct ggcgctattc acggcttagg
    12481 gtttggcgat aatgcaaagg cagcaatcat gacgcgcgga ttagctgaga tcagccgttt
    12541 aggtgtagca atgggtgcca acccgctgac ctttatcgga ttaagcggag taggcgatct
    12601 agtcgttact tgcacaagtg ttcattctag gaactggcgc gcaggcaact tgttaggaca
    12661 agggcagccc ctagaagagg tgctagccaa tatgggaatg gtcgttgaag gtgtgaatac
    12721 tactcaagca gcgatggaac tagcccaaat aatggaaatc gaaatgccca tcactcaaac
    12781 aatctatgaa gtgctgtatg aacaaaaaga aatcaaaaca gccgcaaaag aaatcatgtt
    12841 acgtgatggg aaaaaagaaa atgaattctc attaacaatt ttttgaagga gcgaatgtat
    12901 atgaaagtca aaaaagcagt tattccagcg gcaggattag gaacaagatt tttaccagca
    12961 acaaaagcta tggcaaagga aatgttgcca attgttgata aaccaacaat ccagttcatc
    13021 gttgaagaag cgttgaaatc aggaatcgaa gacatcttga tcgttacagg aaaagctaaa
    13081 cgaccaatcg aagatcactt tgattcaaat attgaattag aaacaaacct aagtgaaaaa
    13141 ggaaagaccg aattattgaa attagtagaa gaaacgacag atgtcaactt gcatttcatc
    13201 cgtcaatctc atccaaaggg tttaggacat gccgttcttc aagcaagagc ctttattgga
    13261 aatgaaccgt ttgtcgttat gctaggcgat gatatcatgg aagatgaagt accattgaca
    13321 cagcaattaa tcgatgatta cgaacgtact catgcttcta cgatcgccgt tatgcaagtt
    13381 cctcatgatg aaacttcaaa atacggaatt atcaatccag gagaagtatt ggaagatggg
    13441 ttatacaatg tcaaaaactt tgttgaaaaa ccagatccaa gcaaagcacc aagtgattta
    13501 gctattattg gacgttactt attgactcca gaaatttttg atgtattgtc cgagcaacaa
    13561 ccaggagccg gaaatgaaat ccaactaact gacgcaatcg atacattgaa taaaacacaa
    13621 cgcgtatttg cccgtaaatt cacaggtaaa cgttacgatg tcggtgacaa atttggtttt
    13681 atgaaaacaa gtattgaata tggtctaaca catccagaag taggtgcacc tttacgtgag
    13741 tatatcctgt ctttaggtaa aaaattagaa gcagaaggaa gcaagatcga tcataatcca
    13801 aaaggaataa cagaaatcaa agaagaaaag aaaaaataac ccttttttct aacgagcttc
    13861 agctgctaat tgaataaaaa gtagttgaag ctcgttttta tttgtttaaa aaacacgaaa
    13921 atgctatcct tatagaaaag agagacaaga agggagtttg atcatgtcag gaggagaaat
    13981 tgcaggattg atcgcagcaa tcgcctttgc gatacttgtc gtttttatcg ttcgtgtgtt
    14041 gttgcaagtt tctaaaactt tagaaaaagt agatcagact gtagcagaag caaatacgac
    14101 gatcgaaatc gtcacaaaag acgtagatct tttgtctaga caagtagaag gcttgctggt
    14161 caaaagtaat gaactgctct tggatatcaa tggaaaagtc gcaacgatcg atccattgtt
    14221 cacagcagtt gctgatttaa gtgaaagtgt atcagaactt aatcattcta gtaaaaacat
    14281 cgcgacgaaa gtcggcggaa tcggtaaaac gacagctaaa gcgacggttg ccggaaaagt
    14341 cggtgggaca gcgatgaaat tttttaaaaa caaaaaacat actgaaacga caggaggaaa
    14401 ataaaaatgg ctaaaaaagg tggattttta ttaggtgcag tagtcggagg aacagccgca
    14461 gcaatcgccg cgttattgtt agcaccaaaa tctggtaaag aacttcggga tgaattagct
    14521 gcccaagcgg atgatttgat ggatatggct tcagattaca cagatatggc gaaagaaaaa
    14581 tcgactgagt taacaggtat tgcaaaagaa aaagcgcaag atctttctca acaagcaggt
    14641 gatctagcag gtagtgtcaa acaaaaagta agtgaatcag cagatgaagc aggaagtgaa
    14701 tctgacgata tcttgttaaa tcttaaagaa caatcagctg acttatctaa tcgtttcaaa
    14761 aaaacggcag aagatatgaa aaaacctgca gatgaattag gaagtattgc taaagatact
    14821 tcagaagata tcatgattga tgtaaaagac gctgctggta aagcaggaga tactgtcaat
    14881 gaaggaatgt cagaagcaaa accagttgca gatgacacaa aagaaatgat gaaaatggca
    14941 aaagaagatg cgaaagaagc taaaaaagaa ataaaagata caatgacaga ttcatctaca
    15001 aatcactaaa atcactaaaa tcacaaattt atagaataaa aaggtgggag cggactagct
    15061 ctcacctttt ttactcttat ttttctaaaa ctagtaattc ttttggtgta gtcgtgaatg
    15121 gcacacagcc atcgcttgtc acatatacac agtcttcgat acgtacccct actttttcag
    15181 gaatatagat tcctggttca agagagaagc acatcccttc ttcgattact aagtcatttc
    15241 cttctacaag ggaagggaat tcgtggacgg ttgttccgat cccgtgacct aaacggtgat
    15301 tgaaatattc gccgtagcca tagctgttga tcacgtttcg tgcgatttga tcaagttcac
    15361 ctgctgtcac acctggacgg acggcttctt gtgcggcaag ctgtgcttca agagtgatgt
    15421 tgtatatttt ttcttggaag tcggttggtt tttgataagc aattgtacga gtagcatcac
    15481 tgcaatagcc attccaaaca acgccaagat cgaataacac aagttcattc ggtgaaatct
    15541 tcgtgtttcc tggtgtacca tgagggcttg cagcatttgc tcctgcaagg actaatgtgt
    15601 cgaaagacat ttgtgaaaca ccttgttttt tcaattggta ttcgatttct gcaatgatcg
    15661 cttgttcagt cacgccttct ttcactgctt taaagccaat ctcaaatgca acatcggccc
    15721 atttaccagc ttcgatcagt ttgtcttttt cctctagagt tttgatcagt tgtagacgtt
    15781 ggatgatgga agctagatca gtgtaagaat ccgttcccgt caagatatca gagatagcat
    15841 cgaagcgctc gacagtcaag gcgttctttt caatccctac attgccaggc tgaccatagc
    15901 gagcacgaag ttcttttccg atgatctccc aagggttttc gctgtctaaa tagccacgga
    15961 catcatacgc ccaactactg ttttgagcat cttcaacttc caaagcagga gtgaaaagga
    16021 aaggatcttg ttcaagaggg ataaaaagag ctagtacacg ctcatgagga tcactttcgt
    16081 atccggaaaa gtaagcgatg tggccagggt cgctgatata agccaattcg acttggtgtt
    16141 gtgccatcca ttgacgtagt tcattgattt ttacgttatt catttggcaa cctactttct
    16201 aaattaattt tgtattcaat aattgtacca ctgatttatt aaaagaccaa cgttctaaaa
    16261 actgaaaaaa gtaaaaattt tcatgaaaac agagataaaa cggttgcaat tcttgagatt
    16321 atttgatatt ctattttaga aatgtaagac gtattttcag aaaacatata aaatatgaat
    16381 aggagaacac aaatggaaaa gcaaacaatc actatctatg atgtagcgag agaggcaaat
    16441 gtttcgatgg ctactgtttc acgtgtcgta aatggcaatc caaacgtcaa accagcaaca
    16501 agaaaaaaag tcttggaagt tatcgatcgc ttggattatc gtccaaacgc agttgcgcgt
    16561 ggcttggcaa gtaagaaaac aacaacagtt ggggtaatca tccctgatgt cagcaatatg
    16621 ttctttgctt ctttagcacg tggtatcgat gatgtggcaa caatgtacaa atacaatatt
    16681 atcttagcaa attcagatgg taacgaccaa aaagaagtca atgtattgaa taacttactg
    16741 gctaagcaag tggacggcgt gatcttcatg ggtcaccata tcacagacga aatccgtgga
    16801 gaattttcac gttcaaaaac tcctgttgta ttagctggct ctattgatcc agatgaacaa
    16861 gtaggtagcg taaacattga ttatgtatca gcaacaaaag atgcagtaaa caaattggca
    16921 aaaagcggca ataaaaaaat cgcttttgtc agtggtgcgt tgatcgatcc tatcaatgga
    16981 caaaatcgtc tgaaaggcta taaagaagca ttaagcgaaa ataacctacc atatagcgaa
    17041 ggattgatct ttgaagcaca atacaacttc aaagatggtc tatctctagt cgatcggatc
    17101 cataatagcg gtgcaacagc tgtctatgtt tcagatgatg aattagccat cggtattttg
    17161 gatggtttat tagaccgtgg cgtgaaagta ccggaagaat tcgaaatcat cacaagtaac
    17221 aattcactac tgacagaagt agctcgtcct cgtttaacaa gtatcacaca accattatac
    17281 gatttagggg cggtatctat gcgcctattg acgaaaatga tgaataaaga agaagtcgaa
    17341 gaaaaaacga ttatcttgcc ttatggaatc gaagaaaaag gatctacaaa ataataaaag
    17401 gacaaccatt cagaggttgt gagcctgccg tttaactctg cgttacaagg agcaatttca
    17461 aaaaacagca tttcatgttt gatgaatatt gtgactcgtg attcggagtt ggcaggagaa
    17521 caccgtttat tcgggaaaag gggttatgac aaaattttgt ttaatccttt ttttttgtct
    17581 taaaaaagag agaaagtggt tgaaattaca tttatcgtat ataaaacaat ccgtgtatag
    17641 atgctgcatt atgttcacca ccatcaaaaa aataagggac tgtgacaata ctattgtcac
    17701 agtcccttat tactgtgtat tctattaaat caatcatctt tctcatctga ttctttggtt
    17761 gcttgattcg ctcttgcata agctgaagcg gtgttccagt aatctgtata gttttcatcg
    17821 gtaccgccaa cgccaaattt aaaggcgctt ttcttcggtc catttttcgc ccatagcgat
    17881 tcaactgttt ttcctggtgt ttggatggat tttttattga cagtaacttt tccaggtgtt
    17941 tgaccagtaa atccagatac ttttttcttc atcacatcag atgataattc gaatttctca
    18001 tcagtgccaa aaatgtcagg ttttgcttgg taagcttgat tgatcagatt ggcaagataa
    18061 ttagccgaac gtatccttgc tttgctgtcc attccagtat tgtcatcgtg ccctgcccag
    18121 ctactgatgg taatagacgg agtagagaca attagccatg aatctcggta ttcattggta
    18181 gaaccagttt ttccgaccca atctgctttt cctagatttc cgtttaagct tgagatggca
    18241 tctttgaatg gtgtagtgat ttttgcattg atcacactgc gcatcatgtc attcatgatc
    18301 gatgccgttg cttccgaata gatccggaca gagttgcttt cgtgtttata aattacattt
    18361 cctgcgttat ctgtgatgga atcgatgata tagccttctt catagacgcc gccgtttgct
    18421 aaagcctgaa atccattggt ctgttgaagc gtcgtgacat ctactgtacc taaaggagca
    18481 gattcaacgc cccaattgtc atttgccggg tagttcatct ttgccaagta gtgctcgtaa
    18541 gcatattgtt tcgatccacc ggaattcaac acatcttgat ataagtgata agccgggata
    18601 ttgttggacc attctagtga ctcacggacg gtttggaagg tgttcgatcc ttcgttagta
    18661 gcattgacga ttttctcgcc agcattttct ccttcttgcc aagttgttgc ataatctgat
    18721 acacgggatt ctgatccgat aagccccatg tcgattgcag gtccataaac aagaactggt
    18781 ttgattgatg agccagcttg acgttctgta tcgaaggcat ggttattttg attctgactg
    18841 taattccgtc cgccaacgaa tccgtagatg cggccagttc tgttatccat caatacattt
    18901 ccggtttcta cttgactgcc agcaccatca tctaacatgt atccataatt ttctacgcca
    18961 ttttgcaatg cagcatagac atctttatcg attgtcgaat ggattgtata tcctttattt
    19021 tggatcgttt gctgtgcctt ttgataataa gcgtcactga ccgatgaatc agacatatca
    19081 gcgttgtctt tttcagccaa ttgttgtgca atgattcttg ttgcttcatt catcacagta
    19141 taatacaaga attctctgtc agtctgttct gcgatttgtt gtggtagaaa gtcttttgta
    19201 aggtcatagg cttttgcttc ttcgtattgt tctttcgtaa tttggccttc acgatacata
    19261 ctgaataaaa caaaatcttt tctggcaagg ccggcagaaa gatcttcttt tagcgcacct
    19321 gtgttcgtgt atggactata cgtgatcggg ctttggggta atccagcgat ataagcagct
    19381 tgcggcagcg tcacatcttt tgcatctaca ccaaagattc ctcttgcagc ttcctggact
    19441 ccagcaatgt tttgtccctt attattacga ccaaatggtg agacattcaa atacgtagca
    19501 atgatctcat cttttgaaaa gtatttttct acttgtgcag aaagaatgat ctcgtttgct
    19561 ttacgtttga atgttgtttc atctgtaagg atctgctgtt tgactaattg ctgtgtcaaa
    19621 gttgatccac cagaagagcc aataccagta gcttcagata ctaatgctcg taaaacagct
    19681 ttaggaacgt aaccttggtg tttatcgaag tattcatcct ctgtgctgat aattgcagtt
    19741 ttcaataaag gtgaaatttt atcagatgaa atagtcgtgc gcatcagatc tgtctggatt
    19801 tttgatatct ctgtattatc tgcataaaca atctttgacg tttctgtaat gtttcctagt
    19861 tctgtctgca gtgctttttt agtcggcaat tgtgtgtctt cgacgagata ggcaaaatag
    19921 ccagcgccta ttccaatacc tagagctgcg aacatcaaaa gaagaacagt aataaaaaca
    19981 gtcaatgatt gaaaaactct aaaaataatc ttaggaacaa accatttttc tttttttctt
    20041 ctattctgtt tttttcgagt atttttgcgt ggcaaaaggg tcacctcaac taatggtttt
    20101 atttcgctaa ttatagcaaa taatagaaca aaaaaacaga caaggctgtt tttggcagga
    20161 atttcttttt taaaagagtt aaaacgtgag aaaatgatct tttttataaa agagttctta
    20221 agcttttctt tagagaaaat ttcagttact atggagaaca gggcaaagaa aaatcaatca
    20281 ttttctttgt atatcatctt accaatatat aggaattgtt agtaacaatt gcctttcaag
    20341 gaggaatcca tccatgttat taggctcaca cgtcagcatg agcggaaaga aaatgctgtt
    20401 aggagcagca gaagaagcag ccagttatgg atcttcgact tttatgatct atacaggcgc
    20461 ccctcaaaac actagaagaa aagccatcga agagatgaat attgatgccg gaaaagcgta
    20521 catggcagaa aacggtctaa caaatatcgt cgttcatgcc ccttatatca ttaatttggg
    20581 gaatacgatc aaaccagaaa tgtttccttt tgccgtccaa tttctgagag aagagattgt
    20641 tcgtgcggaa gctttaggcg ctactcaaat tacacttcac ccaggtgctc atgtaggggc
    20701 aggaacagaa gccggaattg ctagaattat tgaaggcttg aatgaagttt tgacaaaaga
    20761 acagacggca caaatcgctt tggagacaat ggcaggtaaa ggtacggaaa tcggtcgaac
    20821 atttgaggaa ttagcgcaaa tcatcgatgg agtaaaattg aatgaaaaat tatccgttac
    20881 ttttgacacc tgtcatacga atgatgccgg ttataatatt aaagaagact tttcaggtgt
    20941 aatggaagag tttgataaga tcatcgggtt agatcggctt aaagtactcc atatcaatga
    21001 ttctaaaaat ccgcaaggtt cgcataaaga ccggcacgcc aatattggtt ttggaacaat
    21061 cggattcgac acgctgaata agatcgtcca tgatccaatg tttgacaata tttcgaaaat
    21121 cttggaaact ccctatgtcg gcaaagataa aaagaaagct tacgcaccat atggaaaaga
    21181 aatcgccatg ttcaaagcac aaatattcga tccagaagtg tttcctgagt tattagcaga
    21241 agaagtgata ctctaagatt ttctatctga cagcagcaaa aaaggaccag gcacattgcc
    21301 ttgtcctttt ttgtttaaga aacaaaggag tgctttttgc agtctttttc atcatgcatc
    21361 ttctcccccc tctttttaaa tttagacaaa cagggtatac tggtagaaaa gtaaaaaaac
    21421 gaaaagtttc acaagcaagt ggcaaaacgt gattggaagg acgaaaagat gctgaacact
    21481 ttaaaaccct tgatcaacca actaaaaaag aaacatacat actttattct tggcatagcc
    21541 ctattattta caattattcg tttgtattta tatagtaaag caatctatgg aacagacatg
    21601 tcggcaacct atgatgacca gctgctgatt cgctatgctc aaaatatttt ggacggtaaa
    21661 tggctagggg agtatacgac tacaacactt tccaaaggaa tctcctacag cttattttta
    21721 gtacttgcac ataaactgca tctttcttat gcagttttgt tcggcatgtt cagtattttt
    21781 gccagtatat tggttgcact cgcgtttaaa ccgatcgtga agaacaagta tcttctttta
    21841 agtttctatt tttatttttt gttttctccg attagtttta cacatgaata ttccactcga
    21901 acgtatcgta atgccctcgt gattccggct gttgagatca ttatcgcttg tttactagct
    21961 atttattacc gtaaatatag ttccagcaaa aaaatgctgc cgtggttttt agggctagct
    22021 gtttcttttc ctttcttttg gttcattcgc gaagattcca tctggcttct tccttttact
    22081 gtagtcgcgt taggcatcag tttcgtccag atcttgctag gacaagactt ttctttcaaa
    22141 gaagcaaaga agttaaaaga acgaccattt ctcctttcga aacaagaatg gctgaaactt
    22201 tcattgtttt tactccctct agtcggtttc tccatcgtct atcagtggat cgaatataaa
    22261 aacgagacaa catatagtat tgccgccatc aatgaccgtt cgagtggcga atttggacgt
    22321 ttgatgaaac agctgatccg aatagatgac gaaacgaact taaataaaga aaacagtgag
    22381 atatgggtct cgcatgctgc attaaataaa gcattagacg catctcctac atttcatgct
    22441 gtttctactc gtattgacga attgtatacg actcatgcgt ggacccaagg tggaaaagta
    22501 aaagaattac caggagatat cattttttgg gcattgcgag atgtggtaag cgaatctggt
    22561 tattaccaaa acaatgcaaa agaaaccaat gatttttgga aaaaagtcaa tcaagaatta
    22621 gaagaagcct atcaaaaggg aactatcaaa aagaaaaaag aattctatct gatgggatca
    22681 ggggatggaa agacgatgaa tgatctgcca atcgtatttg cttttctgaa atcaggatat
    22741 gcctatgcat ggcgatacaa agatttccat caagggggga attatagttt cggtacgcaa
    22801 gaacaagtcg aacaggccca agaattattg aatcttccac taatggataa ttggatcgat
    22861 agcagtgatc cgagaacacc tgatttccag ttgacaaaaa cagcaaaagt cgcgaatgtc
    22921 atgataaaaa tttaccaagt tctggccatt cccgtgctga ttgcttcaat agcagggata
    22981 ttacttatga tactaggaac cgcatttgca aaagctgatc gtgcctatta tagttcctct
    23041 ttgattgtag tcaccggatt aatactgaca caattggttt ttcttttcgg cgtctcgtgg
    23101 ttttgttctt atgcgccaga aaaaaaggat tattttttaa gtgtctacac tggcgcaggc
    23161 gtgccattaa cgcaattcat cgtagtaaca agcatgctag ttttcagtaa attgaatctg
    23221 aaaaaagctt tatcttccaa ataaaaacat actaagatta gaggagaaca tcattgacga
    23281 atgatgttct cctctatttt ctatttttag attatagtga agagtgaaag aaagttcgaa
    23341 caaagccttg aggactatga gtccctatca aaaaccgaac ttctttactg tcattttaga
    23401 atcaggttta cgtatgttgg ggccatgagc ccgtgtatag gaaattggaa tcgaaatgaa
    23461 gcagtaaagt tctttcaaaa cttaaaaagg aggaaaacaa atgccttaca ttcttgcatt
    23521 agatgtttca atgggacaca gctacagtgt cctatacaaa gacgacacct gcttatttga
    23581 agacgagatc gaacacactc aaagaggatt tcatgcatta ctcgaagaga tccagcactt
    23641 gcctgaacgt ccattgattg tttttgaagc aacggggatt tattccagac cagtcgaaaa
    23701 gttctttcaa gatcatcact tttcttactg tttgttaaac ccgttagaag cgaaaaaaca
    23761 gatggaagaa aacaccttat gcagttggaa aacagataaa tcggatgccc atcggctcgc
    23821 acagactcat cttaaaaatc aacgtcagcc aaaagaagta caaagcaatt tttacttaga
    23881 gatgcgcgat ttagcccgtt tttatcaaga aattgagaag aaaatcactc gtctgcgcat
    23941 ggatttacac aactgccttc agttgacttt tccagagctt gaacagttct ttttaaacag
    24001 gctgacccct tacgcattga tattgatccg tctttttccc catccagatt ttgtattggc
    24061 atctactcgg acaaaaataa aaaataaact gatcaacgaa acacggaaaa aaatctccgc
    24121 gaatcgtgcc gagcaaaagg cagatcaaat catccactat gcacagtgtg cctatccagc
    24181 tgttgaaaaa gacagtatcc actgtcaaaa aacgatctat tacgctgaac ttctccaaga
    24241 tttactcgaa caaaaagaag cactggcgac acaaatgatc aaaaaagccg aaggctctcc
    24301 ttgctttttg ttgtaccaaa cgttccctgg gattggcgca ctcacagccg ctttattgct
    24361 cggtgaatta ggagatatca cacgatttaa aacccataaa caattgaatg cctttgtcgg
    24421 aatcgacatc cgacggtatc attcaggtaa gtatactggc caagaccgca tcaataagcg
    24481 tggtaatccc aaagcacgga aaattatttt cttcaccatt cgaaatatga ttcgccagca
    24541 acgcgcagct cctaatcata ttgtcgatta ttactacaaa ttaaaaaagc aacctatccc
    24601 taagaaagat aaggttgcca cagttgcttg tatgaacaag cttctgaaat gtatgcacgc
    24661 tatggttagg gcacatacag agtacgacta tgcgtacgcg gtctcagtgg accattaact
    24721 aaaccactta cagtatacca cttctttgcc cagatcgaaa attttttacg aacggggctt
    24781 tatttggtgt gccttgattt tgtcgctttc gtttatgagc tatttttctt acacctttta
    24841 cttgactaat cgtaggaaag ggaactgtga caaaagtctt gtcgcagttc ctttttccga
    24901 ataaacggtg ctcaaaagct tgcatctgcg gtaataagtc caaccaagtt aaaaacataa
    24961 aaagctgttt ttacttggct gttcttatta cttgatgcaa ggcagctttt tcacaacctc
    25021 tgatacacgg tgttccagaa gtaacttctt cggaaataag ccgaaatttc acaaaaattt
    25081 gaaaagcaat tttcttaaat tccttcttat ttcttgaagc tgcccacttc tgacacaacc
    25141 tcctttgtgt aaaagaatca gtttctactc gatttcttct atcgtttcct gcaaacgctg
    25201 ttcatatttt ttgtttcctt tatataaatc accaatcgtc catgccaaca ggagaaggac
    25261 aagaacaatg attacataag cgatttgatc agccaatgct agttgagctg aggtggcttg
    25321 ctcgccgaag aaactttcga tttgacctgg taatcctcgt aaattcaagt aagtcagact
    25381 aatcacagag aaccagccaa gtagttttac aacaaatgaa tttttgaatc ggctccccat
    25441 ctccgcttgg ctattggtca tcatcagtaa aggaatcatc gagaatggca atgcaaaagc
    25501 cagaaaaact tgggattcat tcatcaagtt attcaaagcg gtatgttctt cgatcgttcc
    25561 ttttgtactt gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca atcgtgtgac
    25621 tagtcttctt agccaaattg ggatacgcat atggatgaag ccttccatga tgacttgacc
    25681 ggtcaacgta ccagtaatag tagagttctg gccagaagca agaagtgcta cagcaaataa
    25741 agtagacaag ataccagatt ttgctacagc aatcaagata ccattgctca tagctgatgg
    25801 atcagataaa gcttcataca gaccaaaaaa ggaaggatct tggacagaac cggttttgaa
    25861 tacagccaca cccatgatca atagcagtgc attgactaga aaagccattg acaactggat
    25921 attagaatcc caagtagaaa agcgtaccgc ttgtgctaca tcttcttcgt cttggtgatc
    25981 gatcttccga gtttgagaaa ctgctgaatg aagatataag ttatgcggca tgactgttgc
    26041 accaataatc cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg gtgagtctga
    26101 gaatgtgtga ctgttaggga taaatccggc aaacaaactg tgccaatcag ggttagacaa
    26161 gccaacttga tagacgaaaa cgaataaaat gacgaaaatc aagcagacaa ccaaagcttc
    26221 gattttacga aacccgactt ttgtcagcaa taaaaggacg aaaacatcta acacagtgat
    26281 aaatacggca attaccaatg gaatatcaaa aagcaggtag agggcgattg ctgcaccaat
    26341 gacttctgcg atgtctgttg ccataatggc taattcagtc aagatccata aaatgacacc
    26401 caatgtctta cttgtccgtg ctcggattgc ttgggccaaa tccatttggg tcacgattcc
    26461 taattttgct gccatatatt gtaaaagcat agcaatcaga ctagacatca aaataactga
    26521 catcaataaa tattggaaat tttgtccacc agtgatcgaa gtggaccagt tacctggatc
    26581 catataacca actgctacta atgcacctgg tccagagtaa gcaagaagtg ttttaaaaaa
    26641 actttttccc ttgggtacat ccactgttcc atttacttca gctaaagata aaccatttgt
    26701 atgttcaata agtcgatgct tatgaaattg tttgttggat tgattattca agaaaaaacc
    26761 acctttccta tttatctacg attacattct acacctcaga aagaaaaaat taaagaaaaa
    26821 gatttggtat gcctaatttt ttttcgtggc gtgtcttgct ggttaatgaa agaaattttg
    26881 ttcttctcta aaaaaaccac tattttttgc atgaaactaa agaatacgcc tttctttttt
    26941 caataaactt tagtaaaata aatcattgag agcaattcgg aaatagaaag gagagattta
    27001 tatggtatca acaggtattg tcgtacttat tgctattatt gctttgctaa tcggcgcagt
    27061 aggtggcttt ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa atcctcccgt
    27121 taatgaggac atgttgcgat caatgatgat gtcaatgggg caaaaaccat ctgagaagaa
    27181 aattcgccag atgatgcagc aaatgaagaa ccaagggaaa aaataggttc tgacccaaag
    27241 actttttgcg ggctgatttt ttcagtccgt tttttatttc gtaatttgtt agactattca
    27301 gactattcag caaaaaagac aagcacagac aatcctatgc tcttgataaa gacaaaggag
    27361 gagtaaaatg tcgattttta gaaaattagg ttggtttttt cgacaggaaa agaaacatta
    27421 tttaattggg attttctccc tattcgcagt ggccttggta cagttagctc ctccaaaagt
    27481 gattgggatc atcattgatg agatcgccga taaaaattta tcaataaaga tcatttcctg
    27541 gtggatctta ttattgatcc ttgcagctct cttacaatat ctttttcgtt atatctggcg
    27601 gacgaatatt tggggaagtg cagctcggtt ggagaaagaa cttcggcagc agctattcaa
    27661 acattttaca gaaatggaca atctctttta ccaaaaatat cggactggag atttgatggc
    27721 tcatgcaacg aatgatttga atgcaatcca aaatgtagca ggagctggta tcttgacctt
    27781 tgcagattca gtgatcacgg gcggaacaac gatcattgcg atgattttgt ttattgattg
    27841 gcgcttaaca ctgattgcgc tgattcctct gcctttacta gccgttactt cacgagtgct
    27901 ggggtcaaaa ctccacgatg cttttcgtga ttcgcaagaa gctttttcta gtatcaatga
    27961 taagacacaa gaaagtatta ctgggatcaa agtcattaaa acatttggac aagaaaaaga
    28021 agatctcgcg gattttgaag aaaagattga agatgcaatt attaaaaaca aacgagtcaa
    28081 ttttttagac tccttatttg atccattcat taccttgatt atcggtctgt cttatgttgc
    28141 aacaattatt ttaggtggaa agtatgtctt ggaaggcacg atctcaatcg ggcagatggt
    28201 ctcatttatt agctatatcg gtatgcttgt ttggccgatg tttgcgattg gtcgattgtt
    28261 caacgtattg gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt tgcatgaaaa
    28321 gacgcatatc gtggaacaac tgggtgcagt gcagactccg gcaaaaggcg agctgaatgt
    28381 agctatttcc tcttttaact atccgaaaga agaatcacca gcattagaac aaatcaaatt
    28441 tgcgatccaa gaaggagaaa cattaggtat tgttgggaaa actggtgcgg gaaaaacgac
    28501 aatcatcaaa ttactgatgc gtgaatacga tgattatcaa ggaaaaattc aatttggcgg
    28561 atgttcgatc aaagaatatt cattgaatgc tttattagga tcaatcggct atgtgcctca
    28621 agatcacttc ttgttttcga tgaccattcg agataatatc cgtttttcta atccaaaact
    28681 ttcagaagaa gaagtagagc atgcggcttc tttagcattt atcaacaatg aaatcaagga
    28741 attaccaaaa ggttatgata ccttagttgg agaacgtgga gtctctttat ctggcggaca
    28801 gaaacaaagg atttcgattg ctcgagcatt gatcaaaaat cctgaattgc tgatcttaga
    28861 tgatgcactt tctgcagtag atgccaaaac agaagaagct atcttgcata atttaaaaga
    28921 aataagaaaa gagaaaacaa ccatcattgc agctcaccgt ttaagcagcg tgatgcatgc
    28981 aaaagaaatc attgttgtcg atcaagggaa aatcattgaa cgaggaaccc atcatgagct
    29041 gcttgcatta ggcggctggt atgaacgaat gtgggagaaa cagaaattag aagcgaagat
    29101 agaaggaggc gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa atcgattcca
    29161 cttaaagaac agaagaagat catcaaacga ctgatgaagt tcgccaaacc ttttagaaga
    29221 acgttcatcg ttgctatctt atttgcgttt gccctgtctg tgatcaatgt cctattgcca
    29281 aggatcatcc aaacttttat ggatgatcat ctggctaaac agtcggctac gacacaagtt
    29341 attttgtttt ttgctggtat gtacttgttt ggagtcatag tcaaaagtat catttggttt
    29401 ttccaatggt atttgtattc catggcttca ttgaagacct atcagtatgt tcgggtaaga
    29461 ttatttgaaa agctgcatac actaggtatg cgttattttg atcagacacc ggcaggatcg
    29521 actgtttctc gagtgacaaa tgacacagaa acgctatttg aattttggta tgtctttttg
    29581 atggtattga ctgggatctt tgcagtcgtt tcttccttca ttgccatgtt tcaaatcaat
    29641 ggagaaattg ccttgtggac tttggtgttc ttgccgattt taggaatcgt catctggtat
    29701 taccagaaat tcagttctcg tatttaccgg aatatgcggg aaaaattaag tcagctgaac
    29761 acgaaattga atgaatcgat ttccggcatg cgtattatcc agcagtttcg ccaagaagct
    29821 cgtttagcta aagaatttga acaagtgaac aacgagtatt tagacacgcg ctatgcgatg
    29881 atcaaaacaa attcgattct gttaagtccg atcatcaatt ttctatatgc attggcgatt
    29941 gcgttggcgt tgactttgtt tgggatcgat gcgctgcatt ctcccgtaga agttggactg
    30001 atctatgcat ttacgaccta tgtccaggga ttttttaatc caatgtctca aatgatggac
    30061 tttttgagca cctttacaga tggaatggtt gccggtagtc gtattttgaa aatcatggat
    30121 acagaagaat atgcaccgca acaaaatcct ggagcagatc aagcgattac ttcaggtaag
    30181 atcgagtttc gcaatgtaag cttttcttat gatggagagc acaatgtatt gaacaatatc
    30241 tcttttgtcg ctaatccggg agagaccgtt gcattagtgg ggcacaccgg aagcggaaaa
    30301 agctcgatca tcaacgtatt gatgcggttt tacgaatttt atgaaggcca gatcttgatt
    30361 gatgatcatg acattcgaga atatccaatg gaagagttac gtaagaaaat gggattggtc
    30421 ttgcaagatg ccttcttgtt ttatggagat atcgcaggaa atatccggat gatgaatcca
    30481 actatcacag acgaacagat tcgccatgca gctgagtttg ttcaagcaaa caagttcatc
    30541 gatgaattac caaaaggata tcactcaaaa gtgatcgaac acggtgccag ctattctagc
    30601 ggacaacgac agctgatcac ttttgcgcga acaatcgtga cagatcctaa aatactagta
    30661 ttggatgaag cgacagcaaa tatcgatact gaaacagaag gattgatcca agaaggattg
    30721 acaaaaatgc gacaaggacg gacaacgatt gcgattgccc atcggctgtc cacgattcgg
    30781 gatgcagaat tgattttagt tttagacaaa ggtcatatag tagaacgtgg gaatcatgaa
    30841 acacttctag cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag cagtggtgtg
    30901 taaaaagtgg ttgtgacagg gctccgttca gctttaggtg ataagaaaaa aattgtaaaa
    30961 aaggctttcc agatttttgc ataaatttgg cttatcaccg ataagctggt actggaacac
    31021 cgtatattag aagttgggac agtagcatag tagcattttg acctaagcgc tagagtgaaa
    31081 ataaaacaga gcttcccaga ttcttcatct gtgagcgtta gtgtcgtaat taccagattt
    31141 aaaaaaaatc acgacagcta gtcagaagtg cccaatagcc gctaagaaag cgttgacaaa
    31201 agtgcttgct ttcgataata taaagaaaac gatacaactt ggatagtgat tgcctgatgc
    31261 aagctaaacc tgatgagtct cccatggttc catggacggc tttttgggtt tttctttttg
    31321 ttttggatac agccctatat atcatggaaa ggagtaaaac atgctgattg agaaagtact
    31381 gaataacaac gtcgtaataa ccagggatga aagaaatcag gaaatgatcg tgatgggaaa
    31441 aggtttggca ttcaaaaaga aagtgggtga cccgattttt gactcatgca tcgacaaaaa
    31501 atttcagttg acaaatgacg gactttcaca acagttccaa gaattggcac aagcgattcc
    31561 tcttgagtac ttagaaattt cttgtgaaat cattgaatat acaaaagagg ctctacaagt
    31621 ccagttgaat gattcggtgt atattgcatt gacggatcat ttgtatacgg ctatcgaacg
    31681 tcacaaaatg ggaatatttg taccaaatgt attgctgtat gatatcaaac aactatttcc
    31741 taaagagtat gccatcggga aaaaaacagt gaagaaaatc tcggataaat accacatcaa
    31801 gttatcagaa gatgaagccg gatttatcgc attgcattta gtcaatgcac aaggcattca
    31861 aagccaacaa gacaatacct acctgtttac tacaacaata caggatattc taaatatcgt
    31921 ccgctattac tataagatta catttgatga agattcagtc catttttttc gattcgtcac
    31981 acacctacgt ttttttctga cacgcgtcct agatggagaa aaatcagaag tgggagaagt
    32041 ttctgaggaa ttgtaccagg ttattcaaaa aaaatataag gatacgtttg gttgcgttga
    32101 gaaaatagca gactttttag ccgagatatt caactaccag atgtccacgg acgaaaaaat
    32161 gtatttaacg atccatattg cgcgtttagt gcatgagaat cgttgtaaat aaataaaaaa
    32221 aggaggataa attaacaaga acgcatgaaa taagggtagt gacattcagt tggctaaacc
    32281 ttccactaat aaattggagg gaatagaaat gggaaaatat caagaattag cgagcaaaat
    32341 tgtagaaaat gtcggcggga aggaaaatat caatggtttg acacattgta tcacaaggct
    32401 tcgtttcaaa ttaaaaaacg aagaaaaagc aaatgatgag atcttgaaaa atatggacgg
    32461 tgttgtgacg gtaatgcgag cgggaggaca gtaccaagtt gtcatcggga atcatgtgcc
    32521 agtcgtgttt gaagaggtta taaaagcagg aaaccttact tttgatgagg ctgtttcaac
    32581 agaaaaaatg agaccgtttg atatgcttat tgatggaata tcgggctgtt tccagccatt
    32641 ccttgctatt ctagctgctg gtgggatgat tcgtggactg acagcattac ttgtcttctt
    32701 aggcgcattt gaccgcggtt ctggcacatt tgttatgttt gataatatcg gtgattctgt
    32761 attccaattt atgcctgtta ttatcggttt aacagctgcc cgtaaattta aagtaaatga
    32821 attcgtaggg atgttgcttg gggcagcatt aatgaatcct agcttgtcgc tggaagcttt
    32881 gagtggtgca gcagaagcac cactgactac catattttct ggcactattt ttgaagcacc
    32941 tatttatcaa acagtattcg gcattccctg gattgcgcgt aactacgctt cttctgtcat
    33001 tcccattatc tttattgtgt tgttagctag ccaaattcag aaaccaatta aaaagttagt
    33061 acctgagatg atcgcgaatt tcttcgttcc attcttcacc gttcttatta caatgccctt
    33121 aggtttttta ctcgttggac cagcattcac attcgcaact gatattttaa tggctggatt
    33181 tgaaacactt ttagcacttt cgccagttat ttatggagca atcgtaggct tcttctggca
    33241 aattctagtg atgtttggtc tccattgggc aatcgtacca atgggattga tgcaattttc
    33301 tgtgaatggt tggcaaaata tcatgacacc tgttgcagta gtgtcttttg gacaaactgc
    33361 agcacttact gcactctact ttaagcttag aaacccaaaa gacaaagcaa tcgcaatacc
    33421 agccattgtt tctggtattg tggggattac agagccagct atttatgggt ttacattgcc
    33481 acgtaaaaaa atatttattt ttacctgtgt cggtggtgcg ttagggggcg cgtatagtgg
    33541 attaatgaat ttaacttctt ggaatccagg tggattaggc atctttacaa ttcccaacta
    33601 tattagacca gatggtgatt taacggatgt gattaatgta ttgattggaa tcgccattgc
    33661 gatggtggta tcctttgcgc tgactttttt cttttggaag gaggaagcag gtgaaacaga
    33721 tgatagacag aaaagatcag gtaaagaaat tgtaaaaaca ccgatacaag gacaaatcgc
    33781 tccactgaat gcagcaaagg atgcagcttt tgcacaagga acactaggtc gaggaatctt
    33841 gatctaccct gaaaaaggag aagtacgtgc cccttttgac ggaacagtca tgaccctgtt
    33901 cccgacgaaa catgcaatcg gaatggtttc tgaagcaggt ctggaattgc tgattcatgt
    33961 cggattggat actgttcagt tggaagggaa atattttgaa agcttggtgc agcaaggaga
    34021 aagagtgaaa caaggcgatc tgttgctccg cttcgacaaa aagaaaatcg aagaggaagg
    34081 atacagttta gaaacacctg tgatcgtcac caattacacg gattatttgg atattttgga
    34141 aaatcaaggg gagacggtcg ggccagacga tgcacttatc accgcactga cctgaacggt
    34201 taggtgaaag aatggactag gcttaattat tctttttagt ccacttcttt attttcaaaa
    34261 ttgaaaggct ctttcatgat ggctgagctg atagggcgtt tatcaaattt ttgtcctaca
    34321 gaaattagat ggactaaatt ctgttgataa ttttctgcaa aaacacaact atataacacg
    34381 tctagaagat aacaaatcga tgtattgatc gtaaaattac caattttcga atacagtcgt
    34441 tcgcgtgtcg taagtcgcaa agcggctgta ctcattgaag atagcgttga atcaccgata
    34501 ctggtcaaag ctaaaattgg aacatgattt tgtttcaata tttctgcggt acgaaggatc
    34561 acattgtttt cacccgtata agaaatcaga atcccgcaca tatcactcgt gcaattgtac
    34621 gcttcataga acgattcacc aaaagtggtg acaatcgtga catccatttt gatgcgcttc
    34681 attttcagca tgaaatcttg agaaatcaaa gtattcgagt tactggcgaa aatcacgatt
    34741 ttttttgctt gaagcattag ttgtttggct ttttgcagtt ggtcatgatg aatgagagaa
    34801 agtgtatctt gaatcgtcat ctgttctaaa gaagccattt tatttgcgat cgtcataata
    34861 ccgtctgtgg tggaaaaagg aagattcgca tctacgtctt gaaaattccc ttcaagatag
    34921 gctgtttcag ctaaaaaagc attacgaaaa ctgacccagc catcaaaacc catttttttt
    34981 gctacacgga tgagtgtgga cgggtggaca taacatcgct cagcaatctc ttggatggtc
    35041 atttcgccaa gtgctggaga atcgcttaag agaaactgaa tcacttcatt ttctgaagga
    35101 gaaaaggaga tttctttgat tttttcacgt aataacatgt agataccttc ttcttgtttt
    35161 tttacgtaaa ttataacttt tttgaaaatg atttgtaaac ggatttatgt tatatagctt
    35221 aataaagaca ttttttgaca agatatttac aaaaatccag tagaaaacac taacttttgt
    35281 aaatgttact ggaaagaaac gaatttctat tgtttgtttt gtttacaaaa aagataataa
    35341 aagtgtaaaa agtaaaggag tgagaacatg gcaaaaggaa tcaaaatcgc tacgattggt
    35401 ggaggcagca gttacacccc cgagttgatg gaaggattta tcaaaagata tgaggaatta
    35461 ccgatacgag aaatctggct cgtagatgta gaagaaggaa aagaaaagct tgagatcgtc
    35521 agtcagatgt ctcagcgtat gtgggatgct tctccgtatg aagtcaaaat ctatgcaact
    35581 ctggatcggg aagaagcatt gaaagatgcg gattttgtta cgactcagtt tcgtgtcggt
    35641 caattagatg cacgaatcaa agacgagcgg atccctagtt attatgggat gattgggcaa
    35701 gaaacaaatg gtgctggcgg gatattcaaa gcttttcgta ccgttccagt ggttttggca
    35761 atcgttgaag acatgaagcg tttatgtcca aatgcttggc tgatcaattt cgcgaaccca
    35821 agcgggatgg tgacagaagc agttatcaga tatggcaaat gggataaagt gataggatta
    35881 tgcaatgtgc cagtaatggc gatgatgaca gaaccaacaa tgcttggcaa gaaaaaagaa
    35941 gagctgatct acaagtttgc cggtctaaat cacttccatt ggcacaaagt agcagataat
    36001 aaaggaaatg accgcacggc agaattgatt gatcgtttat acgatgaaga caatggattg
    36061 ccgaaaaata tctttgatat cccgtttttc aaagaacaat taaaacaaat gaacatgatc
    36121 ccttgtggct accaccgcta ttactaccgt gaagaagaaa tgctggaaca tgctttagaa
    36181 gaatatcaaa cgattggtac aagagcgcag caagtgaaag aaacagaagc agagctgttt
    36241 gaattataca aagatccaga attggatcat aaacctgaac aattgcaaaa acgaggtggt
    36301 gcttattatt cagatgctgc ctgtgaaacg attgcttcga tctatggaaa taaagaaaca
    36361 cagatcgtgg tatctacaaa gaatgatgga gctgttcctg atctgccggc agattgtgta
    36421 gtagaagtca ctgcctatgt tggcgcacaa ggcgcacgaa atgtagcgtt tggttccctt
    36481 cctgctgctg aaagaggctg gttgcaagta atgaagaata tggaattgct gacgatcgaa
    36541 gcagctgtga ctggtgatta tggaacagct ttacaagcgt ttacgatcaa tccgcttata
    36601 cgatcaggaa agacagcaaa acggatcatg aatgagttgt ttattgcaca tcagcagtat
    36661 ttaccgcaat tcaaggaaac aatcagccgg ttaaaagcag aaggaatcga agtccaagat
    36721 gaactggcaa gagaattatc aagcaataca catgtaatgg aggaagtatg atgggattca
    36781 gaaaagattt tttatggggc ggagctacag cagccaatca atgcgagggc ggttatgatg
    36841 aaggcggacg tgggttagcg aacgtagatg ttgtgccaat cggagaagac cgttttcctg
    36901 tcattactgg aaaaaagaaa atgtttgaat ttgatgaaaa tcatttttat ccagcaaaaa
    36961 ttgcgatcga tatgtatcat cattataagg aagatattgc tttatttgca gaaatggggt
    37021 tcaagactta tcggatgtcc attgcttgga gtcgtatttt tccaatgggc gatgaaaaag
    37081 agccaaatga atctggtttg aaattttatg aggatgtctt taaagaatgt caaaagtacg
    37141 ggatcgagcc gcttgtgacg attacacatt ttgattgtcc gatgcatttg atcaaagaat
    37201 atggtgcgtg gcgttccaga gaactcgttg gtttttatga aaatctgtgc cgagtcatct
    37261 tcaatcgcta taaaggactt gtcaaatatt ggttgacatt caatgagatc aatatgatcc
    37321 ttcatgcacc attcatgggc gcagggctgt attttgaaga aggagaaaat gaagaacaaa
    37381 tcaaatatca agcggcacat catgagttgg ttgcaagtgc catcgcaacg aagattgctc
    37441 atgaagtaga tccggagaac aaagtaggct gtatgctggc agcaggagca tattatccat
    37501 acacttgcaa tcctgaagat gtttgggctt ctagaaaatc agatcgagaa aattatttct
    37561 tcattgatgt ccagtcacga ggagaatatc ctgcttatgc attgaaagaa atggaacgaa
    37621 atggcattca gatcaaaatg gaacctgggg atgccgcatt gctgaaagaa catactgttg
    37681 attttatctc cttttcttat tattcttctc gagtttcgac tactgacaac gaactcttgg
    37741 aacagactgc aggaaatatc tttgcttctg tcaaaaatcc atatctggaa gcaagtgaat
    37801 ggggttggca aattgatcca ttaggcttga gaataacgat gaatgatatt tatgatcgtt
    37861 atcaaaaacc actatttatt gtagaaaacg gcttaggtgc agtcgatacg cccgatgaga
    37921 atggttatgt ggaagatgac tatcgaatcg actatctggc agcacatatc caggcaatga
    37981 aagatgcagt agaattagat ggagtcgatc tattaggcta tacgacttgg ggatgtattg
    38041 atttagtatc agctggtaca ggtgagatga agaaaaggta cggattcatt tatgtcgatc
    38101 gtgacaacga aggaaatggc acattagccc gttcgaagaa gaaatcattc aactggtata
    38161 aaaaagtaat tgattcaaat ggagaaaatc tttcgaatga ttgatcaatg atttataaaa
    38221 gtctattgtt tttcagcgga tttttcgagt ataataaagc tattcgagaa acgcaagcag
    38281 aacaatagac tttttttcgc attggctgta aagtatgctt acttgattaa gtaaaggaga
    38341 atctacatat gtatgatgta atcgttattg gcgcaggtcc tgctgggatg actgctgctt
    38401 tatatgcttc acgttcaaac ttatccgtat tgatgatcga acaaggcgcc cctggcggcc
    38461 aaatgaacaa tacggcagaa atcgaaaact atcctggttt cgattcaata atgggtcctg
    38521 atctagctta taagatgtat gaaggtgttt caagatttgg gacagaaaat gcttatggaa
    38581 tcgtgcaaga tatcaaggat cacggcagcc acaaagaagt tatttgtgaa gataaaactt
    38641 acgaaggaaa aacagtcatt atcgcaacag gctgtatcca ccgtaaatta ggcgtacctg
    38701 gcgaggaaga atttgctgga cgtggcgttt cttactgtgc agtttgtgat ggtgcgtttt
    38761 tccgtaacaa acgtctagta gtgatcggag gaggagactc agcagtagaa gaagccatct
    38821 atttgacaca atttgcttct gaagtagtca tcgtccaccg tagagatgaa ttacgtgcac
    38881 aaaagatcat ccaagaccgt gcatttgcga atgaaaaaat ttcctttatg tgggatacag
    38941 tcgttgacga aatcgttggg aatgaaatgg tagtgacagg tgtgaaagca cgtaatgtca
    39001 aaaccggcaa tgagtttgat ttggcagcag acggagtatt catctatgtt ggactggatc
    39061 cattaactgc accatttaga gaagcagggt tgacgaacga agctggctgg attccaacag
    39121 acaacgaaat gaagacgaat ataccaggcg tttttgccat cggagatgtt cgtgaaaaag
    39181 atttgcgaca aatcactact gctgttggtg atggtgggat cgctggacaa caagtgtata
    39241 aatacttgga atctttagaa gaaacaactg cataagttta aaaagagtgg aagaacggtt
    39301 taattgttct tttactcttt tttttcgcgt gaaatctact gaatccgatt tatgcgaata
    39361 cttgcaaccg tgcttgataa aagaatcctt tcttgattta agcgtttttt ggtcctcata
    39421 ctttcatatc taataagtga atgctatact tattaagaac catatttcag aaagaggtgt
    39481 tattttcatg tcttgggaac aagtgtatga acaatgggta catgaagaaa atttagaaga
    39541 aaacttaaaa aaacaattaa atgatctggg tgaagatcct gaaaaacttg aagatgcttt
    39601 ttatgcaccg ttggaattcg gtactgcagg catgcgggga atcttagggc ctgggatcaa
    39661 tcgaatgaac atctatacgg taagacaagc aacagaagga cttgcacgtt tcatggatac
    39721 acaaggcgaa gaaacaaaac gccgaggtgt cgctatcgct tatgactcac gccatcaatc
    39781 accagaattt gccatggaag cagccaaaac attagcacat cacaatatcc catcttatgt
    39841 attcgaaagt ctacgtccaa caccagagct ttcttttgca gtccgtcatt tgaagacgtt
    39901 tgcgggaatc atggtaaccg catcccacaa tccagcagct tacaacggat ataaagtcta
    39961 cggagaagat gggggacaaa tgcctcctgc agacgcagat gcattgacga aatatgtccg
    40021 tgaagtcagc aatccgttaa aagttgaagt attatctgac gaagaagctg aacatagcgg
    40081 attgatcacg atcatcggag aagaagtgga tgccgcttat ctagaagaaa tcaaagcagt
    40141 aacgattgat catgaattgg tggaaacaat ggggaaagat ttgaaacttg tgtacacacc
    40201 acttcacgga acaggaaaaa tgttgggcga acgtgcgttg aaacaagctg gattcgaaca
    40261 attcgtacta gttccagaac aagctgtcgc agacccagat ttcactacag tcaaatcacc
    40321 aaatccagaa gaacactctg cttttgagta tgcgatacga cttggcgaga aagaaggcgc
    40381 tgatttgctg attgcaacag atcctgatgc agaccgttta ggtgcagccg ttcgtttgcc
    40441 aaatggctcc tatgaagtat tgaccggaaa ccaaatcgga gcactgtttg ctcaatatat
    40501 tttggaagca caccaatcgc gaggcacttt acctgaaaac gcagcggtat tgaaatcgat
    40561 cgtttctagc gaattaccta cagcaattgc ggaacactat ggggctacca tgttcaatgt
    40621 attgacagga ttcaaattca ttgctgaaaa aattcaacaa tttgaagaag atcattccca
    40681 tacttttatg ttcggattcg aagaaagtta cggttattta gttaaacctt tcgttcgcga
    40741 caaagacgcg atccaagcat tgctcttatt agcagaagta gcagcttact ataaaaaaca
    40801 aggcaaaacg ttgtatgatg gcttgcaaac gatttttgaa acctatggct actacgcaga
    40861 aaaaacgatt tctgtgacaa tgagcggaca agaaggttct gcgaaaatct ctgctttgat
    40921 ggaaaaattc cgtgaagaag caccaacaga atttgccgga gcaaaagtca tccaaacaga
    40981 agacttcaaa gagttgacga gaacagaccg tgaaggaaac gtagaaaaac taacgacacc
    41041 gccatctgac gtattgaagt atacgctgga agaaaatggc tggatcgcta tccgtccttc
    41101 aggaactgag ccgaaaatca aattttatat cggtgtaaaa ggaaactctt cagaagacgc
    41161 agctgaaaaa gtagccgctt atgaaacgat tatccaaaag atgacagaag aataaaaaaa
    41221 cttgagactg atggaaaaag tggtaaactt tagaagtcag tcttttttca agttggaggt
    41281 gaatggattg ccagacacgc aaaaaacaaa agtagagaaa gtcagccaac tattcaaaat
    41341 gttgagtgac cccactcgtc tcaaaattct gctgtatctg aaagacgggg aacaaaatgt
    41401 gacagcaatc atccaagcag tggaaatgga acaatctgct gtatcgcatc aactgcgttt
    41461 attaagagaa aaccatgtcg tcaagtccca tcgtgaggga aaagcgattt tgtatagtct
    41521 tgatgattct cacgtactgg atattttgaa ccagacactc aaacatgtgg aacatcaata
    41581 acgataggtg caaatagaga caaactggac tgtgtcaacg aatcttcttc gctgacacag
    41641 tccagttttt ttaaagaaaa attgttagtc tgtttcttcg gtggtttcag ttccataata
    41701 actatggtct tctaggtcta aagcattcaa aataagcgaa gctgtttctt caatagataa
    41761 agaagcgaca ttgattacta cgcagcccaa tttttcataa agatcattcg caaaatcgag
    41821 ttcagctcgt attttttcga tatcggaata agcagtatca ggatttaatc cgtaagagcg
    41881 catccgttct ttacggatat tgtttaaaat atctgggtta ttcgtcagtc ccacgatttt
    41941 tttaggatcg atctcccaaa gctgtttggg aatatgtgct tgtgggatca aaggtagatt
    42001 ggcaactttt aaatttttgt tagctaaaaa caagctcaaa ggcgttttgg aagttcggga
    42061 tacgcctagc aaaacgacat ctgcttctag aaacccacga ggatctttcc cgtcatcgta
    42121 ttttacagca aattccattg ctttgatccg tttgaaatag ttctcattca ataaatgaag
    42181 ggcgccaggt tgccgaatgg gcttgatacc agttaagcgc tcgatttctg atatcggcgg
    42241 agtcatcaca tcaaaactaa ataattgatg ttcttcacaa aaagaacgcg tcatctcgat
    42301 caattcttga ttgatcaatg tgtgcaggac aagagctttt tcttctagtg catcttgcag
    42361 tgcctgcatc aatgttttct catctgtcac aaatgttctg cggaataaat tgaactcaac
    42421 gctaggatat tgggccatag tggcttgcgc tagttttgtc gcagtttctc cagcagagtc
    42481 ggagatgaca aaaaaagtaa cgatttctgt agaatcggaa gacatggata aaacctcact
    42541 tgcatttttt ttatgtttat tataccatac tctagaatgt gtacttcgta atcattttga
    42601 tttggaggaa taagtcatga aaaaacagac gttgatcgaa caatcactag aaacattgaa
    42661 aaataacggt tttaaatata ctaaaaaaag agaagcattg ttaacttatt tagtgaagcg
    42721 caatcgctac gtatccgcta aagaagtttt tgactatatg aatcaggaat tcaaaggagt
    42781 aagttacgat acgatttatc gaaatctcca tgattttgca gaaatcgatt tactggaaga
    42841 aaccgattta aatggagaga tgaagtttcg ttttcattgc tgtcaagggg aaatcggcca
    42901 tcaccatcac tttatctgta cggtgtgcgg aacaacgaaa gaaatccata tgtgtccaat
    42961 gaattatttc gaagaacagc tcggtggatg tacgatcgaa gggcatagat tcgagatttt
    43021 cggaagatgt gaaaattgtc aataaggatt taaataaaat aacatttgat aattttaaaa
    43081 aaaagcaaga tttccttgac ggtcaaaaca gcattagata taatatttac aggacagtct
    43141 tgttttttga gatatatttc aataacaatt acggttactt tttaagctcg gagggaggga
    43201 attaacatgt caaaaacagt cgttcgcaaa aacgaatcac ttgacgatgc tcttcgtcgc
    43261 ttcaaacgtt ccgtgtcaaa agcgggtact ttacaagaat ctcgtaaacg tgaattctac
    43321 gaaaaaccaa gtgtaagacg caagaaaaaa tctgaagcag ctagaaaacg caaaaaattc
    43381 tagttctgtg atgaattgga gttggtagtg tgtcactact aagtagatta aacgatgaca
    43441 tgaaaacagc gatgaaagcg aaagataagg aatctttaca agtcatccga atgatcaagt
    43501 catctataca aaacgagcag attaaagaag gtcacgactt gacagaagaa gaagaattaa
    43561 cggtgttgtc tcgcgaaatg aaacagcgac gtgattcttt gcatgagttt gaagaagcag
    43621 gacgtgacga tttagctgaa aaagtaaaaa gtgaaattgt gatagtcgaa aaatacatgc
    43681 cggaacaact ttccgacgaa gaaattcgac aacttgttca agaagcaatc acgcaaactg
    43741 gtgcttcatc aatgaaagaa tttggcaaag tcatgggtgc gatcatgcct aaagtcaaag
    43801 gaaaagcaga tggaaaccaa gtaaatgcca ttgtgaaaga actattacaa tcttaatttt
    43861 atcatttaaa ctgcggacaa tcattgtccg cagttttttt gtgctcgtaa aaaagagatc
    43921 taaaaaatag ctagacgaaa gaataggatt ctttcatcta gctgtttttt tcctgtttaa
    43981 tatttcttat gttaaattgt gtttgatctg gttattcttc tggtagttca tttgcttctt
    44041 gtttataccc tgataggcga taggcgtaat agaagagtaa gctgccagca tacccaagta
    44101 acgttgggac catccagcca agtcccaatg cagcaaaagg aacgtgatga tgataaaatt
    44161 cgagcatgct tgctacaact ctttgttgac tcactaaagc aggcgaattt gccagcatat
    44221 ccaatacggc gggaacagca gcaaacaaca tcgttacttg atagacaaat ggtgtctttt
    44281 ggaaaaattt tgctgttaag gaaagcaaaa tcaacgcaag cgacaatgga tacaaaagca
    44341 tcaatactgg taaagaccat tggatgatat tgtccaaacc agcatttgca acgacaaaag
    44401 agacgaaagt cgttaaccgc agccatgtca tataactgac tttagggaac agtttatgga
    44461 aatcttgtgc aaaagaaaca acgagtccca tagcggtagt gaagacacct aatgtgacca
    44521 tcactcctaa gaagctagcg ccgaagttac ctaaataaaa attagtgatc tgagaaagtg
    44581 cagttccgcc attttctgat aaagacatct tattcaaact catcgcaccg agaagaacaa
    44641 gaccaaaata gatcatgact tctagcgcaa cgctcaggct accagctttt gctgttaatt
    44701 ctgtgacttt tttatctttg aaaccaaggt cacggactgc atggacaaaa gtgacactta
    44761 aggcaagcaa ggctactgca tcaaccgtgt tataaccttc taggacacca tttgttaaag
    44821 cgtttgtttg gtaagtatct gcagcagatt gatttagatt tcccattggg tggaataaag
    44881 aaacgacaaa aatgattgct aataatgcaa gaaacaagga gtttagatac tttccgacgt
    44941 atttgaccaa ccgtgtggga tggacagtaa ggaaataagc aagtaaaaag aaaagagcag
    45001 agaataaaaa catacctaat gaattatatt tttcaggcaa aaatggttta gcagccattt
    45061 caaaagcagt agcagctgtc cgtggtgtac cgaaaaatgg tccgatcgtc aaatgaacaa
    45121 ggattaagaa caaggcagca taccattttc cgacaggttt agctaagtca tacagaccat
    45181 cactttttgt cactactaca gctaagatag ctagtaatgg gaaaagagcg cctgagatgg
    45241 caaatcctac gccagcactg acccagtttg cgccagccat ttgacctaag tggacaggaa
    45301 agattagatt acctgacccg aaaaacatcc cgaagataag tgaactgacc atcaatagtt
    45361 gtttccatga ataagttttt gtttcttcca taaatttttc ctccaataat tatcattttg
    45421 attgacgata taaacgttgc agcaattagt aatgccacta atatcagatt ccatgaaaac
    45481 caccacctta aaatataaaa aaaagccctt ttccacttgg gaaaagggct ctagattcta
    45541 gaacggtacc accttttttc gcttaaaaaa gcttcttcat tcacggacgc tttttgataa
    45601 agttatccgc tagcctgata atggaggcag ccacaatgat ctacgttttc gacattgact
    45661 cggaaatgat gttcgtctat tcgatttatt gtcatttctc acttacatga ctcactggga
    45721 ataaatcgtt gaatagccta ctgttttcct cgacgctttg ctgtatgttt atttgttgac
    45781 gtaataatac acctgttttt tttgaaatgc aagaatattt taactatttt gttttctttt
    45841 tttgaaacaa gaaaaatacc tagagaatac ctctgaatga aacggaatat tttgttctcc
    45901 actttcatat aacttacatt ctcagattct ttttatcaaa acgagacctt tagcgaatgg
    45961 aaacagctat tttttaacgt ttcttggtac gtatggttaa acgactcttt tacaaccttt
    46021 tcttatttgc tatatagttt cttctgagta tggtaaaata ggaaagaatg aatcgaaaaa
    46081 aaggggatgt ctatttttga cggaagagtc tagttcttta gagattagat tgacagctgc
    46141 tgatgatgtc agcatgcttt taggtgcaca cgacaagcat ataaaactga tcgaggaaac
    46201 aacggaaaca accatccata cacgtggaga aatcattcag atcattggtg aacaatccca
    46261 gatttccttg gctgcctcag tcattcggac attacaggaa ttgatcaaac gaggaatcca
    46321 cgtaagtaca ccagatgtcg tgacggcatt gaaaatgggg caaaaaggta cattagatta
    46381 ttttgttgag atgtatgagg aagagatcgt caaagaccgt aatggaaaac ctatacgggt
    46441 aaaaaacagc ggtcaaaaga aatacgttga atcggtagcc aaacatgaca tcgtatttgg
    46501 tgtagggcca gccggaacag ggaaaacgtt tcttgccgta gtattagcaa ttgctgccct
    46561 aaaaaaaggc gaagtgcaaa aaatcatttt gacgcggccc gctgtagagg caggggaaaa
    46621 cctaggcttt ctacccgggg atctgaaaga aaaagtggat ccttatctgc ggcctgttta
    46681 tgatgcttta taccaaattt ttggattgga tcatacgaac cgattgatgg aacgaggcgt
    46741 aatcgaaatc gcaccattag cttacatgcg ggggcggaca ttggatgatg cattcgttat
    46801 cttggacgaa gcacaaaaca cgacagtcgc tcagatgaaa atgttcttga cccgtctcgg
    46861 ttatcaatca aaaatgattg tcaatgggga tacaagtcag atagacttgc ctcgagggac
    46921 gacaagtggt cttgttcatg cagaacgaac actaaagcaa atcaaaaaaa tcgattttgt
    46981 taattttgaa gcaagtgacg ttgttcggca ccctgtagtt gccgaaatca tcaaagctta
    47041 tgaaaaggcg gatcttcatc aagaataagc tttgcttagg aggataagca catgttgaat
    47101 aaaacactgc agaaactaca agaaaaactc ggaaaggcag cattgccttt ccttctgttc
    47161 ttgtttttta tcattgtctg cggcgttaca tttagtagtg tcaaacaaaa ggccaatgat
    47221 ttcagagaag gccaagtagc agaagagagt atccgtgcga ataaaacgat cgagaacacg
    47281 gaagaaaccg aacaaagaag aaaactggca gcagaagcag tgactccaga atatacttat
    47341 caaaaagatc tggcagatga ccagaacaat cggatcaagc aattattcga attgatcgat
    47401 aagacgaatg ataccatcaa caaatcttat aatgacaagg tagataaggc gaaggaagat
    47461 gaaaatatcc cgaaacctac tacagaagag cggattgcta cactgaagaa aaattttgaa
    47521 aacgtgaatg cagaaaacgt tgcgttttac caaaaactgc cgaacaattt ttattcgacg
    47581 atatttgaaa tgactgaaac ggaaattcac acggttaggg atgaaagttt gaagctcatt
    47641 gacgaacaga tgagtaaaca agttcgtgaa agcgaattgg aagcttttaa acaagaagca
    47701 gaagatcaga ttcagtattt gaatgtcaca acagagcagc aacaaatgat tcgctactta
    47761 gtggatcaag gcattgtagt caatgatgta ttgaatgaga aaaagacaga agaattaaaa
    47821 caatctgcac gagaagctgt acaaccagtg atgatcttcc aaggagaaat cattgttcgc
    47881 gaaggaaatc aaattgatgc ttcagcgatg aaaaaattgg aattactagg attgacgaat
    47941 caaacgactt cgattttccc actcattgcc atggttttag ctgttttgct tcaaatcggc
    48001 gtactgattt ataattcact tcaattccaa aatgcaggag aaagaacaaa atacgtgctt
    48061 ttctatgtga cagctatgtc agtcagcata gtgctgatga aatttttcca attattccaa
    48121 acggaacaag ctacttatat cccgctgttt tatcccgctg cttttgcacc acttgtttta
    48181 agctttttct tgaaccgaag agcagggatc atggctggta ttttccaagc agtatctgca
    48241 cttttcattt tttatgattc gattggcacg aactttctaa cgattatctt gatgtcttat
    48301 gttttctctg ggctcatggc aacgattgtc agacgcaaac ggatctcaga acaaggatta
    48361 tctgctgcca tgtgggtcat tatttttcct gtgatgatgg atgtcatttt gatcgtctat
    48421 caaggaatga gctttggtga tgcaacgact tggttaatgc ttgtctgcgg gttcactggt
    48481 gcactcttct cattcttgct gacgatcggt ctacacccat atattgaatt gatggtcaat
    48541 gatgacagtg taattgtatt aaatgaactc agcaatccaa atcatccttt attgaaacag
    48601 ttgctggaag aagcgccagg tacttatcat catagtatga tggtagctaa tctaagtgct
    48661 aacgctgtag cagaaatcgg cggacgttct ttgctaactc gagtagcttg ctattatcat
    48721 gatatcggca agatcaaaca tgctagcttt ttcgttgaga acttgccttc aggagcggaa
    48781 aacccgcaca atttcttatt gccagaagac agtaaacata ttatctttgg acatgtgaca
    48841 gatggagcca aaatattgga agattataat atgccgcaaa tggtgatcga tatttgcaaa
    48901 cagcatcatg gaaccacgct aatgcgttat ttctacatca aagcgaaaga acgcaatccg
    48961 gaagtaacag aagagcaatt ccgttatcct ggaccaaaac cgcaaagtcg tgaagcaggg
    49021 atcgtcaata ttgctgacag ttgtgaggct gcagtacggg caatggatca tccgacgagc
    49081 gaaaaaatcg aaagttttgt ccataatctt atcgaagagc gtatttccga tggtcaatta
    49141 gacgatagtg gactgacctt gaaagaaatc cgaaaagtgg aaaaatcatt gatcagcggt
    49201 ttaagtagca ccttccattc tcggatcaaa tatcctaaaa tgaaaacaga agttgagaag
    49261 ataaaggaag aacaagaaga atagaaagaa gcgatgtata atggatatta cctttatgga
    49321 tgaaacaaac caagtctcag aagagaaaat gaaagaaatc gatgacttgc tgcaatttgc
    49381 agcatcttat ttaaagctgc cagaggaaac agaaatgtct gttactttca tggacaatgc
    49441 ggcaatccaa gtaatcaatc gtgattaccg tggaaaagat gcaccgacag acgtcatcag
    49501 ttttgctctg gaagaagaag gcgaagacga aatcccagtg atttttgaag atggagagaa
    49561 tcctttgcct cgtgtactag gagacatcat gatttctatt gaacgtgctg ctgaacaagc
    49621 agaagcttac ggccattcat atgatcggga acttggcttt ttagcggtac atggattctt
    49681 gcatatcaac gggtatgacc atatgacacc agaagatgag aaagaaatgt ttggtttaca
    49741 gaaagagatc ttggatgcct atggactcaa aagataaaaa cgtagagaaa aacaaacatt
    49801 tcctgatgtc tgtggaattt gctgtccaag gagtcaaaac cgtttttgag gaagaacgca
    49861 atatgaggaa acatgttgcg ttcggtatcc ttgctttatt tatgggcgtg attttccagc
    49921 ttgatcggat agagtggtta tggctgcttt tagctgtttt tctcgtttgg atagtagaga
    49981 tcatcaatac tgtttttgaa aatgtagtgg acatgtttac ggattttcat tttcatccga
    50041 ttgggaaaaa aatcaaagat atggcagctg gagcagtttt agtgaccgct tgttttgctg
    50101 tcatcattgg gttgatttta tttgtcccta aaatatatca tttgttgttt aattaaaaag
    50161 gagaacgtat atgccagaac ataaatcagg gtttgtcgca atcgtcggcc gcccgaatgt
    50221 aggaaaatcc acattattga accgaatcgt gggtcaaaaa attgccatta tgagtgacaa
    50281 ggcacagaca actagaaata aaatccaagg agtatatact acaccagaag ctcagctgat
    50341 ttttatcgat acaccaggaa tccataaacc aaagcatcgt ctaggagatt ttatggtaga
    50401 agcagcttac agtgcgttga aagaagtgga tgcagtctta tttatgatca gtgctgatca
    50461 aaaacgcggc cggggagacg attttattat tgaacgtcta aaaaacgtgc attctccagt
    50521 ttatctgatt atcaataaaa tcgataagat ccatccagac gagttgctgg gaatcatcga
    50581 agattattcg acgcagatgc cttttgcaca agtgattccg atctctgcta cagaaggaaa
    50641 caatgtcgaa cgcttaatgg atgtattagt cagtgaaatg ccagagggcc cacaatattt
    50701 tccagatgat caagtgactg atcatcctga atactttatc gtttctgaat tgatccgtga
    50761 gaaagtcttg tttttaacac gagacgaagt gcctcattct gtcgcagtcg ttgttgattc
    50821 gatgaagcga aacgagaatg ataaaatcca tatccaggcc acgatcatcg tcgaaagaga
    50881 tagccaaaaa ggaatcatta tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa
    50941 agcaagaaag gatatcgaaa atcttttagg agacaaagta tttctagaac tttgggtcaa
    51001 agtccaaaaa gactggcgag acaaaaaagt ctatctgcaa gactttggct accgaaaaga
    51061 cgaatactga tattctgaac ttcttatagg ttaagtatag aaataaactg gataaaaatc
    51121 cgcacgttga gaaaaacggc ggaagatcga aacataaata atctggatcg gcagaatctg
    51181 cttttccaga tttatttttt aggagggaga atatgagcca gcttgcagaa tcgaaaggct
    51241 tgattttatc tagccgtaat tacaaagaaa aagataaatt agtcaaaatc tttacggaat
    51301 cttctggcaa aatgatgttt tatatcaaag gaatccatcg gcaaaatcag ccattagctc
    51361 cggctttgct cccctttacc gaagcagtct atatcggtaa ttttcgaagt gaaggtttgt
    51421 cgtttttaag cagtgtcaaa gacgtccatg cgtttcggaa catccaagaa gatatctttt
    51481 tgagcggtta tggtacctat ttgctaaatc tagtagatgc agcaatcgaa gatcatcaat
    51541 atgacccgca tttattccaa tttacgcaac aagctctgca gcggatggat caaggagatg
    51601 atgcagaaat catcacgaat atctttgaag ttcaaatact gcaacgcttc ggaatcgcgc
    51661 caatttggac gcactgtgtc gtatgcgggg aaacaaaagg aaaatttgac tattcttcca
    51721 aatctggcgg tgtgatatgc gagaaacact ggccaatgga cgaacaccgt tatcatgcag
    51781 atcctcgcgc tgtttatttc gttcgaatgt tttcggctat ttcttatgat aagatcagtg
    51841 gaatccactt gaaagaagaa acgaagcagg caatccgtga cctcattgac cagttgtatg
    51901 atgaatatgt cggtattcat ctaaaaagca aaaagttcat tgatcaaatg aagtcttggg
    51961 cacacgtatt aaagacgcca gagaaaaata tgcctgaaaa agagatacct gaaaaagaag
    52021 aataaatgaa agatacaaca aaaaaatcaa aactatttga caaatacagg gacttccgtc
    52081 tattattaac cgtagattaa atagatcgtt cactgagagg atctattata taaacaagaa
    52141 aactgcaaaa tgaatacgta cattgaccaa gatcagtaaa aaacacgtgg tttagcgagt
    52201 tcgggagagt ggaagccgga cccaaacgat ttttgaagag gcacttgcga gtacgataaa
    52261 tgaagctgcc gaatctttat tcggaagtag ggtggaaccg cgaaattatt cgtccctatg
    52321 tctgtaatca gacataggga ctttttgtcg ttttctttcc tgtattctta ttgacagcaa
    52381 cgcttaaaaa ttgaaagggg caaaacagat gagtaaaaaa ttgaccgtcc aagatatgat
    52441 tttaacatta caaaaatttt ggtcatcaaa cggctgcatg ctgatgcaag cttatgacac
    52501 agaaaaagga gcaggtacga tgagcccata tacattctta cgggcaatcg gaccggaacc
    52561 atggaatgct gcttatgtag agccatcccg ccgaccagct gacgggcgtt atggagaaaa
    52621 cccaaaccgt ttgtatcagc atcatcaatt tcaagtcgta atgaaacctt cacctgaaaa
    52681 tatccaagag ctttatttag aaagcttgcg tcttttagga attgatccat tagaacacga
    52741 tattcgtttt gttgaagaca actgggagaa cccatcgatg gggtgtgccg gtgtgggttg
    52801 ggaagtatgg cttgatggga tggagatcac acaattcacg tacttccagc aagtcggagg
    52861 actaccttgt aaaccagtta cttctgagat cacttatgga ctagaacgtc ttgcttcata
    52921 tattcaagaa gttgaaagtg tctatgatct ggaatgggct gatggtgtga aatatggcga
    52981 gatcttcaaa caaccagaat atgaacattc caaatattct tttgaagtaa gtgaccaaga
    53041 cttgctacta agcaactttg accgttttga aaaagaagct aaacgctgta tcgatgaaaa
    53101 tcttgttcat ccagcgtatg attatatttt gaaatgcagc catacattca atttattaga
    53161 tgctcgcggt gcagtatccg taacagaacg agcaggttat ctttcccgta tccgcaacat
    53221 ggctcgtgct gtagccaaaa tctttgtggc agaacgcgaa aaattaggat tccctttatt
    53281 gaataaaaac acagcaaatc cagaaaacat gaaggaggct gaataagatg gcaaaagact
    53341 tattacttga aatcgggctg gaagaaatgc ctgctcatgt tgtcacacca agcatgaaac
    53401 aattagaaga aaaaacagca aaatttttag atgaaaacca attgacttat gatacgatcg
    53461 aaacgttttc tacaccgcgc cgcttagcaa tcaaagtaac aaacattcca gaacgtcaag
    53521 cagacagtga agaagaagtc aaaggaccag ccaaaaaaat tgctttggac gcagatggaa
    53581 actggacgaa agcagctgaa ggatttgtcc gcggacaagg tctgacgaca gatgctatca
    53641 cttttagaga attaaaaggc gtggaatatg tctatgtaac gaaatacact cacggcaaag
    53701 aagcgaaaga agtattgaca ggtttagcag atgtcattaa aagcctcact ttcccagtaa
    53761 cgatgcattg ggcagaatac gattttgaat atatccgccc tattcactgg atcgtggcat
    53821 tgttagatga cgaagtaatc ccgtttgaga tcttagatgt caaaacaggc agagcctctc
    53881 gaggacatcg tttcttaggc gaagatgtga catttgcgca tgcaaaagaa tatgaagaaa
    53941 aattgaatga acaattcgtt attgcttcac cgcaaaaaag acaagaaatg atcgttacac
    54001 aagcaaatga attagccgaa aaaaatcact gggttattga tctagacgaa gatttactag
    54061 aagaagtcaa caatttagtc gaatatccaa ctgtcttttc tggcgacttc gaagaaaaat
    54121 atctttctgt tcctgaagaa gtactagtca cttctatgaa agaacatcaa cgttatttcg
    54181 aggtacggaa cgatcaaggt atgctgcttc ctcattttat ttctgctcgt aatggtgatc
    54241 gggtaaaatt ggaaaatgtg gttcgaggca atcaaaaagt attgacggct cgtttagaag
    54301 atgcggaatt cttttataat gaagacaaga aagcttcaat cgaccaatac gttgaaaaac
    54361 taaaaactgt tactttccat gaaaaaatcg gtagtatcta tgagaaaatg cagcgggtac
    54421 aagtcattgc ccgtatcatt ggcgaacaag taggactatc tgaggaagaa ttgatggatc
    54481 tgcaacgagc tgctgaaatc tataaatttg atctagtaac caacatggta ggggaatttc
    54541 ctgaactaca agggatcatg ggcgaaaaat atgctctgct gaaaggcgag aaacctgctg
    54601 tagcaacagc gattcgtgag cattacatgc ctatatccag tgaaggagag ctacctcagt
    54661 ctacagtagg ttctgtttta gcgattgcag ataaattaga tagcattttt tctttctttg
    54721 cagttgggat gattccaagc gggtcaaacg atccttatgc cttacgtcgc caagcatacg
    54781 gtgtcgttcg gatcatcgaa tcgaaaaaat gggcctttcc tttgtctgtt ctgcaagaaa
    54841 cgattagtga tgttatcagt aaagatacgg atcgtttcgg cattggttta tcagaagggc
    54901 aacaacaggt gatcgacttt atcaaaggcc gtctgcgcca attattgaca acgaagaata
    54961 ttcgtcatga tgtcatcgaa gctgttttga atgcagaaca aaaagatctg acaaaagtat
    55021 ttgcagcagc acaattgttc aagcagcatc ttgcagatga agacttcaaa ccatcgatgg
    55081 aagctttgac acgtgtcgtg aacttagcga aaaaagcgga gcttgaaaaa caatcagaag
    55141 tagaccctga actgttcgaa aatgaagcgg agaaagaact gcacaaagca gtagaggcaa
    55201 tgaaaaaaag ctttgctgac caaacgatca acgaaaatta tgatcagctt gtaagccttc
    55261 gcccactgat cgaaaattat tttgatcaga cgatggtcat ggcagaagaa ccagcagtac
    55321 gtaagaaccg attaacgcaa ttaagtcaaa ttgctgccat ggctttgtcg ctagcaagtt
    55381 tagatcaaat catcacaaaa taatttctaa tatgtaaata aaccagagaa taggcacaaa
    55441 ataggctatt tctctggttt atttgttata aatataaata actatacccg tggtgctagt
    55501 taattttttc ctaggtatta tacaagtaat ttgagtcggt caatttcaac tgaattatct
    55561 aaactaactg atctactttt agataattca gttgaaattt attggtaaac tgtcgctttt
    55621 ctttttaaat ggcaaaaatt tttaataata tatgacaatt tagaaatcaa ctataagact
    55681 gattaattca aaatgatctt agaatcattg tatgaacact aaatactata ttgttttcta
    55741 ttaatcgtta tataaacgat tatgaattca aagctacttt ttatgacgtc aatcagagag
    55801 tgtaaaa
//
LOCUS       DANKEF010000016        52671 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000016 DANKEF010000000
VERSION     DANKEF010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.016"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(75..797)
                     /locus_tag="PWZ48_001046"
     CDS             complement(75..797)
                     /locus_tag="PWZ48_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HDL2260718.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQSYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKTVPIDHLLIETDGLHAAEWALGGIFSEKYYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(787..1524)
                     /locus_tag="PWZ48_001047"
     CDS             complement(787..1524)
                     /locus_tag="PWZ48_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260719.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYRNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(1536..2528)
                     /locus_tag="PWZ48_001048"
     CDS             complement(1536..2528)
                     /locus_tag="PWZ48_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2260720.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQETKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDITYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDNPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(2543..3286)
                     /locus_tag="PWZ48_001049"
     CDS             complement(2543..3286)
                     /locus_tag="PWZ48_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260721.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(3360..3464)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(3527..4849)
                     /locus_tag="PWZ48_001050"
     CDS             complement(3527..4849)
                     /locus_tag="PWZ48_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2260722.1"
                     /translation="MKTYDAIVIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYFVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANVVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRNPPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(4836..5801)
                     /locus_tag="PWZ48_001051"
     CDS             complement(4836..5801)
                     /locus_tag="PWZ48_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260723.1"
                     /translation="MTKLIEFKHVQKKYDRKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFNEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(5798..7336)
                     /locus_tag="PWZ48_001052"
     CDS             complement(5798..7336)
                     /locus_tag="PWZ48_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HDL2260724.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKVLNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(7752..8039)
                     /locus_tag="PWZ48_001053"
     CDS             complement(7752..8039)
                     /locus_tag="PWZ48_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260725.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            8173..8841
                     /locus_tag="PWZ48_001054"
     CDS             8173..8841
                     /locus_tag="PWZ48_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2260726.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(9127..9867)
                     /locus_tag="PWZ48_001055"
     CDS             complement(9127..9867)
                     /locus_tag="PWZ48_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260727.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKGNLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKMLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(9997..10944)
                     /locus_tag="PWZ48_001056"
     CDS             complement(9997..10944)
                     /locus_tag="PWZ48_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260728.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQIRSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(11073..11837)
                     /locus_tag="PWZ48_001057"
     CDS             complement(11073..11837)
                     /locus_tag="PWZ48_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2260729.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRGSEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(11841..12230)
                     /locus_tag="PWZ48_001058"
     CDS             complement(11841..12230)
                     /locus_tag="PWZ48_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260730.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            12364..12906
                     /locus_tag="PWZ48_001059"
     CDS             12364..12906
                     /locus_tag="PWZ48_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HDL2260731.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            13054..13686
                     /locus_tag="PWZ48_001060"
     CDS             13054..13686
                     /locus_tag="PWZ48_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260732.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(13820..13945)
                     /locus_tag="PWZ48_001061"
     CDS             complement(13820..13945)
                     /locus_tag="PWZ48_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260733.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYSILWEELAA"
     gene            complement(14079..14975)
                     /locus_tag="PWZ48_001062"
     CDS             complement(14079..14975)
                     /locus_tag="PWZ48_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HDL2260734.1"
                     /translation="MNYYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKK
                     EFSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLS
                     VDEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRATAGNEKYRFITLSSIEHF
                     PLTLANYFFVQKNQLPVTKAFKELAELIGDVNEAYFFDQIEVIVDRIRKNKYKNRRPS
                     IFHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEETAFLKRQIFSELS
                     KKFIPHAFSYIKEYRTVLVSKT"
     gene            complement(15157..16083)
                     /locus_tag="PWZ48_001063"
     CDS             complement(15157..16083)
                     /locus_tag="PWZ48_001063"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HDL2260735.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(16168..16929)
                     /gene="pflA"
                     /locus_tag="PWZ48_001064"
     CDS             complement(16168..16929)
                     /gene="pflA"
                     /locus_tag="PWZ48_001064"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HDL2260736.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGNKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(17173..19404)
                     /gene="pflB"
                     /locus_tag="PWZ48_001065"
     CDS             complement(17173..19404)
                     /gene="pflB"
                     /locus_tag="PWZ48_001065"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDL2260737.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(19681..22131)
                     /gene="parC"
                     /locus_tag="PWZ48_001066"
     CDS             complement(19681..22131)
                     /gene="parC"
                     /locus_tag="PWZ48_001066"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDL2260738.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     VTIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(22143..24188)
                     /gene="parE"
                     /locus_tag="PWZ48_001067"
     CDS             complement(22143..24188)
                     /gene="parE"
                     /locus_tag="PWZ48_001067"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDL2260739.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLTVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEVPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(24354..24788)
                     /locus_tag="PWZ48_001068"
     CDS             complement(24354..24788)
                     /locus_tag="PWZ48_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDL2260740.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEVADDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            24915..25553
                     /gene="plsY"
                     /locus_tag="PWZ48_001069"
     CDS             24915..25553
                     /gene="plsY"
                     /locus_tag="PWZ48_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDL2260741.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(25685..26560)
                     /locus_tag="PWZ48_001070"
     CDS             complement(25685..26560)
                     /locus_tag="PWZ48_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2260742.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNREIFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADAVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(26642..27433)
                     /gene="codY"
                     /locus_tag="PWZ48_001071"
     CDS             complement(26642..27433)
                     /gene="codY"
                     /locus_tag="PWZ48_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HDL2260743.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(27451..28851)
                     /gene="hslU"
                     /locus_tag="PWZ48_001072"
     CDS             complement(27451..28851)
                     /gene="hslU"
                     /locus_tag="PWZ48_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HDL2260744.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALIKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(28865..29413)
                     /gene="hslV"
                     /locus_tag="PWZ48_001073"
     CDS             complement(28865..29413)
                     /gene="hslV"
                     /locus_tag="PWZ48_001073"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HDL2260745.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(29450..30355)
                     /gene="xerC"
                     /locus_tag="PWZ48_001074"
     CDS             complement(29450..30355)
                     /gene="xerC"
                     /locus_tag="PWZ48_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDL2260746.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(30553..31929)
                     /gene="trmFO"
                     /locus_tag="PWZ48_001075"
     CDS             complement(30553..31929)
                     /gene="trmFO"
                     /locus_tag="PWZ48_001075"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HDL2260747.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLDSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(31898..33976)
                     /gene="topA"
                     /locus_tag="PWZ48_001076"
     CDS             complement(31898..33976)
                     /gene="topA"
                     /locus_tag="PWZ48_001076"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDL2260748.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDIVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(34098..34955)
                     /gene="dprA"
                     /locus_tag="PWZ48_001077"
     CDS             complement(34098..34955)
                     /gene="dprA"
                     /locus_tag="PWZ48_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDL2260749.1"
                     /translation="MYQIEENLLKLAYAKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITTHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVVEAGYVIVSGCAKGIDTYGQQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHRFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(35011..35778)
                     /locus_tag="PWZ48_001078"
     CDS             complement(35011..35778)
                     /locus_tag="PWZ48_001078"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDL2260750.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(35771..36631)
                     /gene="ylqF"
                     /locus_tag="PWZ48_001079"
     CDS             complement(35771..36631)
                     /gene="ylqF"
                     /locus_tag="PWZ48_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HDL2260751.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            36892..37290
                     /locus_tag="PWZ48_001080"
     CDS             36892..37290
                     /locus_tag="PWZ48_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2260752.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWVMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            37569..38069
                     /locus_tag="PWZ48_001081"
     CDS             37569..38069
                     /locus_tag="PWZ48_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HDL2260753.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRVIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(38152..38703)
                     /gene="lepB"
                     /locus_tag="PWZ48_001082"
     CDS             complement(38152..38703)
                     /gene="lepB"
                     /locus_tag="PWZ48_001082"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDL2260754.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(38855..39082)
                     /locus_tag="PWZ48_001083"
     CDS             complement(38855..39082)
                     /locus_tag="PWZ48_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HDL2260755.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(39079..39597)
                     /gene="msrA"
                     /locus_tag="PWZ48_001084"
     CDS             complement(39079..39597)
                     /gene="msrA"
                     /locus_tag="PWZ48_001084"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2260756.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(39617..40330)
                     /locus_tag="PWZ48_001085"
     CDS             complement(39617..40330)
                     /locus_tag="PWZ48_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HDL2260757.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(40333..41229)
                     /locus_tag="PWZ48_001086"
     CDS             complement(40333..41229)
                     /locus_tag="PWZ48_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2260758.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(41387..42229)
                     /locus_tag="PWZ48_001087"
     CDS             complement(41387..42229)
                     /locus_tag="PWZ48_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2260759.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEEILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            42361..43014
                     /locus_tag="PWZ48_001088"
     CDS             42361..43014
                     /locus_tag="PWZ48_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDL2260760.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(43078..43605)
                     /locus_tag="PWZ48_001089"
     CDS             complement(43078..43605)
                     /locus_tag="PWZ48_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2260761.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEIDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(43625..44572)
                     /locus_tag="PWZ48_001090"
     CDS             complement(43625..44572)
                     /locus_tag="PWZ48_001090"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2260762.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKEVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(44584..46470)
                     /locus_tag="PWZ48_001091"
     CDS             complement(44584..46470)
                     /locus_tag="PWZ48_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2260763.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSGDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(46635..48707)
                     /locus_tag="PWZ48_001092"
     CDS             complement(46635..48707)
                     /locus_tag="PWZ48_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HDL2260764.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRHADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            complement(48720..48995)
                     /locus_tag="PWZ48_001093"
     CDS             complement(48720..48995)
                     /locus_tag="PWZ48_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260765.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(49357..49812)
                     /locus_tag="PWZ48_001094"
     CDS             complement(49357..49812)
                     /locus_tag="PWZ48_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260766.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(49966..51174)
                     /locus_tag="PWZ48_001095"
     CDS             complement(49966..51174)
                     /locus_tag="PWZ48_001095"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HDL2260767.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPSLKEEGEALLRFAELPSVPLKEESQVWTLLISQLGLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(51286..51618)
                     /locus_tag="PWZ48_001096"
     CDS             complement(51286..51618)
                     /locus_tag="PWZ48_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HDL2260768.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            51766..52641
                     /locus_tag="PWZ48_001097"
     CDS             51766..52641
                     /locus_tag="PWZ48_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2260769.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
BASE COUNT        14428 a        11025 c         9019 g        18199 t
ORIGIN      
        1 attaatcgaa cggtttactt ttatacaaag aaagccaaaa agaggtgtga cacaactagt
       61 gtcacacctc ctttttattt acctaatcta atgaaacgtt ccaagttttg ataagtattt
      121 ttcttaactt cttcaactga tagctctttg atctcagcga ttgtctttaa ctgttcttca
      181 agcagtgaga cataatattt ttctgaaaaa atccccccta aagcccactc tgctgcatgt
      241 agtccatccg tttcaataag taagtggtcg attgggactg ttttagctag ttgccagact
      301 gctgggttct ttttcaaatc aagaccaatg ctgaaataac agcctaaatc aatatagtct
      361 tgttgatagt ccaaagaaga ataccaatga atcaaatagt gattagggta agattggata
      421 taatttaaga tctctttttc gcatcctttt gtatgcagta caacaggttt ctcatttatg
      481 gctgcaaaag caagctgtcg ctcaaatact tttttttgtg tagtcatcgg tacattcgtc
      541 caaatattat ctaaccctat ttcaccgatg atccttgctt ttttcaaaaa aggctcaact
      601 tgttcaaacg tatagctatc tgctttccaa ggatggatac cgtaacttaa ggcttgagtc
      661 ttacttgctg caagctgtct gttttccttc cattcttctg gcgaatcaca attgatgatc
      721 gtcaaaatcg tgtgttcctg ctgaaggagt gctaaactct gactcccatc taaatgacaa
      781 tgagcatcac ttaacatcta attcacctgc caataactga taaatcgttt gcttcaactc
      841 tgattgctgc agtagccatt cacgtgaccg tcgtttttca ggcacttgaa tctcagcaat
      901 cacatgtgcc ggacgggcgg aaagcacaag aatcctgtct gataaataaa ttgcttcttc
      961 gatatcatgc gtcacaagta aagtagtccg ctttgctttt ttaggcagct tcaacatcca
     1021 gtcttgcatc tgccctctgg tcatagcatc taacgctcca aatggttcat caagaagcaa
     1081 gtaagaagaa ggattcatga ttgttcgtaa caaggccgtt cgctgcttca tcccgccaga
     1141 taattgttcc ggcaaaaaat gttcatatcc gtttaaccca aagatatcca gataatccct
     1201 tacttgttct tcattgattg ttagatgatt gattttttga tataacgtaa cgtttcgata
     1261 gacgctcagc caaggaagta acatatcttc ttgcggcata taactaaatg ctgtatggag
     1321 accagttact ttttgatcgt ctactttgat cgttccagaa gtcataggct ggactcctgt
     1381 taaagttttc agcaatgttg acttaccggc tccgcttggc gcgacaatcg atgtaaaggt
     1441 atctttaggg atagtgaaat ggatgtcttc taatattttc aggttttcaa tgactacatt
     1501 caagtggctg atttggattg acatatctat cactcctatt ttataaactg attagtgaat
     1561 agttcttgcg aaggtacatc ttgcttgatc aatccgtttt cctgcataaa agctgtataa
     1621 ttgcgccaaa ctttttcttc catcaatccc caattctcag gattatccgt ataatttttt
     1681 gacaagaatg cttgtgattc ttctaaaaac gtccgatcat tttcttttgc atatttttgc
     1741 aacagtttag cactgtcatt tggatcctta atagcttcct gatagccttt tttcgtggca
     1801 tccataaaag actgtaccaa ttctggatca cttttgatca gttgatcatt cgtaataatt
     1861 accggagtat agtaatctaa ccgttcatct aattctttca atgggatata gtttagttca
     1921 atacccgctt cttttgcttt gatcatatcc caaccttcaa agtaccattg gatatctgag
     1981 gattttccta agctttctgg cccttctcca ttgcttccta ccattgtcag ttttgaaaag
     2041 tccccgccgt ctttttccat cacagctttt aagacagctt cttcacttgg actttgccaa
     2101 cctgcatatg tttttccttc aaaatctttt ggtgaatgga tgttactgtc tgatagggta
     2161 gcgaatccag aagtattatg tttgatcact gtagcaattg ctttgactgg tatattctgt
     2221 ccatctgcgt gagcataagt gatatcttct tgataactta caccaaattg tgctttgtcc
     2281 gcacctacta gaccaatact tgtcgaatta tctcctggtt cgatgatttg gacatttaaa
     2341 ccagcctctt tataatagcc tttttctttt gctaggtaga tgccagtatg attagtattc
     2401 ggtacataat ccaaaacaag tgtcactttt ttcaaatcct ttgtttcttg tttgctactt
     2461 gtttcttttg cagtgccaca agcggataaa agcaacagta gcggtaataa aagccataat
     2521 tttttcattt gatccccttc tcttaaaaaa tataaagcat acgataatac ccttttttca
     2581 ataatgaaca agcgccattc agcaataaac tcaatagaat gacacaaatg atcgcagcaa
     2641 aaactttatc gatctggtag ctgtttttta cgcgtatcat gtaataccct aagcctgctt
     2701 gagcacttag ccattctcca acgactgctc cgctgacaca ataagtagca gccactttta
     2761 aaccggaaaa gaagccggat aaacccgctg gtattttata tattcgataa atcttccctg
     2821 tatctgcccc catcgttttc aaaaagatga tttgctcttt tgatactttc ctcaatgtat
     2881 ccgaaaaaga aactacgatc ggaaaatagc ttatcaagac aacaaggatc acttttggaa
     2941 gcataccgaa accaaaccat agggtaagta atggccctaa caccatgatt ggcaacattt
     3001 gtgaaatcac tagtaacgga taaaaagaca tttctaaaaa cttccaataa tccattgcca
     3061 aggcagtaat aaaagaaaga acgctagcta ttaccaaacc aagcactgct tcatttaatg
     3121 tcgtgacaga atgggaaagt aatacttgtg catctgccgc taatgcagaa aatacagcag
     3181 caggtccggg taaaataaag gcaggtattt tccaaactat gacaacaatc tgccaaataa
     3241 ttaaaatgaa tgccatactg actgctggat aaagattttt cttcattttg ttacctcctt
     3301 tcttctaata tgaataacaa aaaacgcttg aaatgggcac gacatagtaa cccgcttcaa
     3361 gcgtctgcta tgtttctccc ttcaccagta ttaactgaca ggttcaatgg gtctaagttc
     3421 acaacttact ctcatgtcgt ctacgacatc cccagaaaca ggtattcatt ttttaaagtg
     3481 tagcacaaga aggaaaaagc aacaagcgct ttagttatca tgctgattat ttcaataaat
     3541 aggagagatc acttgccaat gtcggatacc ccatgatata gctatccaac tcttttttag
     3601 tcaatttttg attgatgact agtgtcaaat catcgatcaa ttcgtctgct tgctcgctaa
     3661 tcactgcagc accaatcaaa taattttgct ggtcaaaggt caattcagcc ttagcaactg
     3721 gttcattgac acgatgatag gtgaaccaac tggtaagatc catctctacc acttgatctg
     3781 acgaagaagc gtgacttttt gtaacaccta cttcagcaag tttaggactg gcgtagacga
     3841 tcgtaggtat aatcggatat ttgataggtt cgcttgttgc atccgttatc cgcttagcta
     3901 cataatttcc ttcaaaagtg gcgactggtg tcaatttggg ggggttcctt gaaacaatat
     3961 ctccacacgc aaaaattttt ggattgctcg tttgcagata atcattgact gcaattccgt
     4021 gtttatcaaa gacgacattc gcttgctcaa gtgctaaaga ttctgtattc ggctgtctcc
     4081 cagtcgcaca aaaaatcata tctgctacta attccgtctc ttttccgact aaacgatagc
     4141 gagtaccttc tgaaatgatc ttttgggttt caacaccaaa agcaaactga atgccagatg
     4201 cttccatttg atggacggct tcctctacca aagacgcctc aaattctttt aatggccgtt
     4261 gattatgatg gacaatggtt actttgctcc ctgcagcatt ggcaatcgta gctagctcaa
     4321 aagcaatata accaccaccg ataaaaatga tttctttagg cagtacaggc aatgataaaa
     4381 agtctgcact agttttcaag tattcctttc cctccactgg taagatcgta ggtctttgcc
     4441 cggttgccaa aacaaagtaa tccgcatgga agacttcttc gttgacttca atagatgaat
     4501 catccaaaaa acgagcggtt cctgaaagat gatcgatttc agcttctgct aattgttttt
     4561 ttcggctttc tgggactgga tccgtaaatg ttcgtttgaa tttttgaagt tcttcccagt
     4621 tggcagttgg gatctcgtta aaaccttttc cactcaattg ttttacccga ttacgtgcct
     4681 ctactgcact taacaagact tttttaggat cacatccacg gttaggacaa gtcccgcccc
     4741 atagatcttc ttcgaccact agtactgttt tacctgcttc tttcaatcca taagcagcac
     4801 ttaagccgct cacacctgaa ccgatgacga ttgcatcata tgttttcatt tccttcctcc
     4861 tctcgactta aaaaagcaaa gatttcttta cttgtcagcg acccaatact tctttgcttt
     4921 tctactatca caatgcttgg atacaccgaa agaagcgaat atacttcttt cataggcgta
     4981 tcaaagcaaa cttgttgacg agcattttct ggtatttcat ttaagaaata tccattgacg
     5041 actaaatctt ctactcgcca ctctttcgtc tgtttcggtt cattaaagaa atgccgaacg
     5101 tagtcgtttt taggattcat caataactgc tctggtgtgt cacattggat aatttctcca
     5161 tccttcataa atgcaatacg atcacctaac ttgattgctt cttccatatt gtgcgtaacg
     5221 aaaacgatgg tcgtgcccaa ttcttcgtgt aaggacaaaa ccaattcttg taatgagttt
     5281 cttgaaatcg gatctaatgc gctaaatggc tcgtccatca aaatcacatc tgggcttgca
     5341 gcaatagctc ggatgatccc gatccgttgc tgttctcctc cagacaactc tctaggcata
     5401 cgatctcgat aaatatcagg agataagcca actttttgaa gaagttcatc tacacggtct
     5461 gctcttttct gcttttccca acctaacatt tcagggatga cttcaatatt ttgcttgact
     5521 gtcatggtcg ggaacaaagc gatttgctgc agcacatatc ccatattcca ccgcattttc
     5581 tgcagattat attcatttaa cgattttcct ttgaagtaga tgtctcctgc tgatggttta
     5641 gataatccat tgatcatttt caaagtagtt gtttttccac taccggaagg accaaccaaa
     5701 acaaagattt ctcctttttt gattgccaga tttaggtcat cgatgacata ctttctatca
     5761 tacttttttt gcacatgttt gaactcgatt aatttagtca ctgatcttcc tcctttacta
     5821 atccttcatt tttcaggaag attcgtgcta cttctgtcgg ctttttcttt tcgacattga
     5881 cttggtaatt catatcaatc atttgatcct ctgttatttt tccgcttaat ttattcagta
     5941 ctttaacgat ttcaggatgt tctttagcaa aatctttttt catcagcggt gcgccttgat
     6001 aagctgggaa taaccctcga tcatcttcta gagtcactag atcgtactcc ttgagctggc
     6061 tatctgtgga ataggcatcc acaatattga catctccatt attgattgct tggtaacgca
     6121 aacttggctc catgctttgt acacttgaaa aattcaagcc atatgcttct tggattcctt
     6181 tatatccatc tgaacgatca ataaattcca gtgtgaatcc cgctttgatc tgatcagaga
     6241 tagctgccaa atcggaaatc tttttaagtt gatgttcgtt cgcaaaagat ctcttgactg
     6301 ccaaggcata cgtattttgg taagccattg gttttaataa tgtcatatcg aattgcttat
     6361 tcaataattc ttctgcttcg tcatatgttt tttcttttgt caaatcagca cttttcattg
     6421 cttcaggtac cttcaccaag ctttctagga cagtacctgt gaactcagga tagatatcga
     6481 tttgaccttg atccagtgca ttgaacaaaa aactcgtctt gccaaaattc ggttttaatt
     6541 caacttctgt ttttgtgttg tcttcaatca attctttata catttggatc aggatttcag
     6601 gttccgctcc taattttcca gcaatcgtga tggtttcatt tttaaacaag gcagactgat
     6661 ataaagaaac gccgccaaat aaacacacca ctgctgctaa agtaatgatg gaatatcgag
     6721 gtttcttatg ctgcaaccag cgaatcagtc cgctgaatat aattgcaagc aaggcagaac
     6781 tgatcgcacc aatcaatgtc aaaacaggtg tatttcggtc aatacctaac aaaataaatg
     6841 tccctaatcc acctgctccg atcaatgctg ccaaagtagc cgtaccgatg attaaaacta
     6901 aggcagttcg gatacctgag ataatgacag gtaatgcgat gggaagctct actttcagca
     6961 agcgtctcat tctggacata ccaaatgcta ccgctgcttc ttcaatagac gggtcaattt
     7021 cagctagacc aatatacgtg ttttggaaaa tcggaagcaa agcgtaaata acaagtgcaa
     7081 tcaatgctgg aacagttccg atccctacaa atgggataag taaacctaat aaggcaagtg
     7141 aaggaatcgt ttggagcaca cccgtgatct gcaataagac ttctgccatt tttttatgat
     7201 tggctgccca aatcgctaaa ggaatagcaa tcagtgctgc aatcagcaaa gaaatgagag
     7261 aaatggacag atgttgaaat gcagcttcca gcaactcgcc ttttctttca cttaatgttt
     7321 gtataaaatt attcatgcga ccttcctttc tgttattctt taagtataaa catttctttc
     7381 ctttttccca actaacttgt cttgccttaa ccttacagca cgaacaaaaa aaaagaaaaa
     7441 aatacctcta aatatgctga caaaaaagaa tccaagcaac aaagtcggat caactttttg
     7501 gttctcgttt tttcactacg aataccataa aaatctatac gaaattgtca cttggaatca
     7561 gctatatctt ttttacacac acaactttta ttttaataaa aagaaacagc caataaagta
     7621 gaaatctcgt gggggagaac acctttattg gcctgttcaa cgaatattat actgtatcat
     7681 gacacttttt tgccaaaaat gtaaaaaacg gttgttttga catcataaac gtcttgtttt
     7741 cttattttta cttatatcac cactttattt tctatctctt tcactccata aagcggaatg
     7801 agttcttctt ttgactgata ataaacagct ttctcacggt tcttcatcaa aaaaagaaag
     7861 atgtctttca tagcaagtgg tgctttgccg tgttcaaata aaaagataaa ttgatttgct
     7921 ttatttatct ggaccattgt cacaggcacc agtttttgtt cttgagccaa aaaaactcct
     7981 tgatactctg tttttttggg tgattttgta agaattgtca ataagtctgc tgctcgcatt
     8041 tttccactcc ttatttgata aataaaaaga aacagccaaa aataattttt tttcattttc
     8101 ctcttgaagt aagagtataa gtcagtataa taaagaagtt gctgaaaatt ttcaaaagaa
     8161 aagaggctag aaatgaaaaa attaaattta tttacagaat tgtgcggaat cattttaggc
     8221 gcaggaatct acggattagc tgtgacagga atcaatattc catctaagct ggcagatggt
     8281 ggggttactg gtattgcttt gctcttgcat aatctttttg gattcgctcc ttctgtcact
     8341 tcactgcttt ttaatttacc tttattgctt atctcattgt ttctttttgg aaaacacgct
     8401 tttctccgga cgatcgtcgg aacatttgcg ctagttttct ttttacattt atgggagagc
     8461 cttcatatac attttgctgt agatagtcta ctgatcaata gtttgatgac aggtattctt
     8521 tctggcattg gttgcggtct cgtctttcga ttcggcggaa gcacaggcgg aacagatatc
     8581 atttatcaag ccattgaaaa atattttcat attaagattg gcaaaagtct tttcttgatt
     8641 acttttggta ttttagttgt ctccttgctt tatttggatc tcacacattt tctgtataca
     8701 ttacttagct gttctattct atcttataca ttgaataaag tgaaatatct ggaaattgga
     8761 tctgttttgc cccaattgga agcaaaacag actacagaaa catttgaacc acttgaccct
     8821 cttgaacagt tagagcactg attcttattt ttcactactt tatttacaga aatatcatta
     8881 tgtaaactaa aatgatatta ttcagtaaaa tcttctttat aaaaaaataa actatttttg
     8941 caaacgatct gcgaaaatag tttgtttttt tattttgcca ttattttctt acttattggg
     9001 agaaaaaatt cgcttaagct caaaaaaatc tgaggatacg atataaactt ccggatagag
     9061 tataaaataa ggaaacgccg cagcgtttcc tttccaactg tagccgaata taaatttgta
     9121 tatcacttat ttaaaatagc tattttctcc caaagagttt ccttgatcat cgatcaatcc
     9181 ttgtaatgag cgttctaaaa taccatggct ttttttcgat tttttttctt ctgactgctt
     9241 tgtttcctct aacatttttg ttcgattgaa tgttcgtaca gacggctcac catttctttt
     9301 ttcttgatac ggcgtcggtt cagtatcgaa caacagataa gagtcatccg tttttttcat
     9361 cgattcgatt aaatgatcct cagaaatttt gctttctcta gggtctggga taatcgatgc
     9421 gggtacatat ttaggaacaa aataagaacg ctcccgcacc ttcgtgtcaa cagatgcgtt
     9481 tcgtgtagta tgcttggact ctgtccttgt ttcactttgt ctgacagaac gagaggaatc
     9541 accaaaaagt ttttttcgtc ctgtcgatgt gatttctgat tggtaatggc cggtataatc
     9601 cggtaatttt cctttatgct tttccaaatt tcccttacgc ggagctgact tcaatccatt
     9661 tgttgcacga cgcttgcgac ggattttctc ggtgttcgtg ttcgtgaaat gaaattcttt
     9721 catctgctca aacggggact gattctgttc agtcaaaaga tcttcttgtc cgtcaaataa
     9781 tactcgcttc ttgtcgttgg ttaattttac gcctaactca tcatcgtacg caggaaaacg
     9841 gaaatgtttt cgtctgattt ctttcattca aaccatcctt ctcaaaaatg gaacttttct
     9901 caataatagc acagattcat tgttttcagt agcaatgcgc tgattatttt aagaaaatag
     9961 cgatgaattt cataaatata tatatagtaa taagacttaa ttcgttgtct ctctgataac
    10021 tagttcagga aaaaaacgac gataaccttg actcgcccct ttttcttcta ttttttttaa
    10081 caacatttct ccacatactt cacccatacg tataatatct tgcttgatcg ttgtcaattt
    10141 aggtgacctg atttggtcta aaaatacccc atcaaatcca atcacaccaa attcttcggg
    10201 caagtgtcct ccagcattta taatccctct tactgctcct agtgctagcc gatctgaact
    10261 acatacaaaa caagttttat ctgtaaaatc tgcccagttt tctgtcatat atctttctac
    10321 ttctgtcgaa tgatttggaa accgcaagat tttactagga agatcaactt ccttcatcac
    10381 agataaataa ccagtttccc ttgaaatttc aaaaggctcg tccacttcga taccgatata
    10441 aataatcgtt tgataatctc tttctaaggc atattgcgtt gcttttgctg taccatattc
    10501 attatcgctg tctacataat catatccttc agaattttcc ccaaacaaga caactggttt
    10561 ttccaaacga tcgatccaat cgaagtcttt ttttctgaca cctgtgatga tgtatccatc
    10621 acaagaaccg acgtcgaagg catcatttgt aactaattgg agagaataat gcgctttacc
    10681 gatccgttta gcgattccca tcagcaaatt catgtaatag ggctcagttg tgtcgatttc
    10741 ttccagtata aataatttga tgatctgcga ccgattcgtg acaagtgctt ttgcagcaat
    10801 atttggacgg taatccagtt cagccattgc tgagaaaact aactctttca attcgtcagt
    10861 cacctgttcc ggatgattga tgactcgaga gaccgtcatt tttgaaacat tggcacgttt
    10921 tgcaacatct gctaaagtgg gcatgctctc ttcctttcta acatgttgtc attcttacta
    10981 tcaattatac caaattttcc taacagagtt tattatagcc caaaataaaa aacggaaaat
    11041 cctctatcgt catgagtgat tttccgtttc aattatttat acttttttat caaatttttt
    11101 gctaaaaaag tggaagcttc ttccattagt ggtatttgta ccatatgtcg ctgtgttttt
    11161 gaggaacgaa aaacaatttc ttttccgtaa gacttatgcc gatttttttc tacaaatcgt
    11221 tccgtttgta tataagggat tttttcgtca gcttgtccat gccaaaagaa cagaggacgt
    11281 ccagctaact tttccggctg caatgacaaa tcatagctct taacccatga aatcaaatca
    11341 aaatagtctt ttggtacgac ataattatat tccgatccgc gccttacgat ttcttttcca
    11401 tattccaaag gtgcagggga acccatcaaa cacacgcccg ctactatatg aggatgttta
    11461 gccaacaagg cgcatgttgt cattcccccc atcgaaaccc cgccaactgc aagctgatca
    11521 tgtagaagac ctcttttttc taaatgcttg ataagtgtgt caaattcaaa cagatttccg
    11581 tagatactat cccaaaatgt ataggatgga atagaagaaa ccggctgttt gcgctcccca
    11641 tgattcatcg catctggcag gatcacacga atgccctgct gcgcaatttt tcttccttgc
    11701 gtcaaaacta actccttact tgtctgccat ccgtgataat agatgacaac gggaatacgt
    11761 tcctctctta attctttcag aacaacttct aacactggaa tatgatggac agaacgtttc
    11821 aatataattg gattcaattg ttactcacct attttcatta aatttcctga tcgccatagc
    11881 aaccatgctc caacagttgt ctggaacagg ttcagtgtac caaataatat aaatgcagga
    11941 ctgaatggct cgggaaataa gcacagagaa caaagtccca ccactcctaa tgctagcgaa
    12001 aggagtggat aaatagaaaa attcaaaaaa ctggcaacgc taccgaaaat attaccaata
    12061 aacagtatca aaaagaaaaa aacgcttaaa attaaaccat ctgtcagcga catctgaaaa
    12121 acaatgttct ctacctcaat tggctgttga atcgaataga cacctaagta tatactgaga
    12181 aggagactta gcacgtggct ggcacaaaac acacgattga ccgttttcat tttaatcacc
    12241 taacctatct ttataattct attataatca ttttatcatt ttttgtatgt ataaagtttc
    12301 aatttttctt cacttacgat tttttttgat attgttgtta tataaatgaa aggagtttta
    12361 caaatgattg atccaaaaat gttggggatg atcttcctca ttaattttgc ttatatcact
    12421 ctgaacacgt tacgtttcat gttgacaatg aaaggctatc gactgattgc tccacttgtc
    12481 agtatggtcg agatcacgat ttatatcgtc ggcttatccc ttgttttaga tcgtttagat
    12541 aatccactga atttatttgt ctatgcgtta ggttacgcag taggtatcag tgtcggaatt
    12601 ttgattgaag acaaattggc attaggatac atcatggtca caacgatttt gccagcaaat
    12661 agcgcagaag ataaaagttt gccgggtata ctgagagaac acggttatgg tgtgacacag
    12721 acttctgcaa tggggctgga aggtgagcgg ctagttctcg aaatactttc tcctagaaaa
    12781 agcgaacgtg aactgtatcg cctgatcaaa gaaatcgaag agcgtgcttt tatcatttct
    12841 tatgagccaa aatatatttc aggtggtttc tggactaaaa aagtgagaaa acgacaagga
    12901 aaataactca caaacttaaa aactgtctaa tatctaaatt aaattttaaa tgttagacag
    12961 tttttattta ttattttgat taaaaaacat cgacatatac tcatcaattt gttattatac
    13021 tttatatact tatcaattta atttggagat attatggaaa ttcaacgtag aaccactaat
    13081 tttttacatt taggctcttt ttttgcaact ttgacgatct tattttttct atgttattca
    13141 taccttaaga cctcacatgc gccttctagt gcattaggct ggttgctttt ggatcattct
    13201 gatatcacag aagccgaaga aaatccgttt tttttgattt tgtcttctct ttgcggctta
    13261 ctgttttctg tttatttcgg tgcctatttt tttcatcgac atcctggtac gttaaacgaa
    13321 gcttcaagcg tattgattgc atttaaaacg ttttcttttt tagcaattct tttttattcg
    13381 tttaatcaca atacgctttt cttctttatc atgaatggtt tagtcgtgat tttatccatt
    13441 tttacttatt ttattttttc actcattttt agccaattga aatgtccact cttctttcga
    13501 ttagttcctg tcacctgttt tatttacagc ttgttttcct tttttgtcct tgctttaatg
    13561 ccactgacca acccttcgac gatccaagtc gcaggaaatc tgttggatat gctcttcatc
    13621 gtcagtctag gtgtatttat gtggcaaaga aaaaacaaac aaaataaaaa gcattttgaa
    13681 gcctgactat tacgacttca taaatgcttt tttagtttta aacacttcaa tctaaaagaa
    13741 taacccaaga ttggataaat tattaatgac tcgccattcg tttcttagtg attctggatc
    13801 atcaaatgga cttcctaatc taagcagcta gttcttccca taaaatgcta tacttgactg
    13861 aatcataata ttgattattt aaaaatctaa cttttcttat cactacttcc tttgtcattc
    13921 cacatttctc accatttttt tgcattcctt tgtttccaga ccatgtagtg aatccaatag
    13981 gtggtagata ggcaaaatac tcaaataatt cttttaacca taaaggaaga gaaaggctcc
    14041 tcctgcaaga gtcagtataa tttttgctta ctgctctctt aggttttact cactaaaact
    14101 gttctatatt ctttgatata agaaaatgca tgcggtataa atttcttgct cagctcagaa
    14161 aagatttgtc gtttaagaaa ggccgtttcc tccattgtca atgaaggatc caatgttggt
    14221 aattcaatct caaaattgtt tttttcgaca ataggtaatt ctaatgtgaa tgattcttct
    14281 tcctttatag tattcgtcac aagtagagac tgatggaaaa tactaggccg gcgattttta
    14341 tatttattct tacgtatcct gtcaacgata acttctattt gatcaaaaaa gtacgcttca
    14401 tttacgtctc caataagttc ggctaattct ttgaatgcct tcgttacagg aagctgattc
    14461 ttctgtacaa aaaagtaatt agccaaagta agcgggaagt gttctataga ggaaagtgtg
    14521 ataaaacggt acttctcatt tcctgcagtt gccctcagca attctgtctc tcggctgttt
    14581 cttactccat aaaaatacgc ttctttccgt tcatcttcga aacaatacgc ccaaataact
    14641 tctcccatcg ctaatttttg ttcatcaaca gagagtctct tcaattgttc agctatggtt
    14701 tcagcagccg aagtggcttg ttgcaagtaa tctttaggat caaatattgg aaagttcaaa
    14761 tgatatcttc gttcttccct cttgattaat cctgcatcga tcaatagatc aagaaaatgg
    14821 gaaaatttat tttgcgaaaa ttcttttttc aattctctta gaatcgtttc ttgttctcga
    14881 tgttcatata gataatcggt caatgaagaa aaaagcggat catcggtcag tttttgataa
    14941 aagttttcct tattttttga ataataatag ttcatatatg ttctcttttc tatccgtttt
    15001 attttactta aaaggctttt acgtaaaaag gaggaagaac aagagtcata attgactgtt
    15061 attctcctcc atttcttcgg cctttagtat gttctatgtt tctttttata tcaatcgctt
    15121 atccattcat tttgaatgct tcatactacc ctttgattag ctaaatacag ccgtcaattg
    15181 aggtacgact tgttttttac gtgaaacgac acctttaagc aaagcacggt tgttggtcaa
    15241 agttgtttgg aaagcttctt ctactttttc ttttccttta ccagcaacaa gtaattctga
    15301 atcactgtct aagatgtttg tgacaactaa tacgaacaga tcgtagccat ttgctttcat
    15361 ttcatcttcc attgctgctt ctagctctgc ttgtcgatcc atcacttcat taagatccac
    15421 tgtattgact tgagcgatac gaacgttgga actgcccatt gggaaacttt ttgcatccaa
    15481 atcaagaaga accgcagctg atttatcact tagatttgtt cctgctttca acatttcaag
    15541 tccataagtg tttagatcga cttcagcgat tgctgcaagt tcttttgcag ctgcttcatc
    15601 ttcaggcgta catgttggtg atttgaacaa caatgtatct gaaatgatag ctgagagcat
    15661 gattcctgcg atttctttag gaattgttac ttggttttct ttatatagtt ttaaaataat
    15721 cgtacttgta catccaactg gttctgcacg atagtacaat ggattagctg tttcaaagtt
    15781 tgcaatacga tggtggtcta ctacagctaa aatatttact tcgctgatat ctgaaatact
    15841 ttgttggaat tcattatgat ccacaagcat gacatctgca ttttctgctt tagctgactc
    15901 tactactcgt ggtgcagata gctgaaagtg gtctaatgcg tattgtgttt cttcattagg
    15961 tgtacctaga gcaactgctt ctgtttcttt acctaattca ttttgcaagt gtgaaaacgc
    16021 aatggctgcc ccgattgcat ctgtatctgg attttggtga ccaaaaacta aaatttttga
    16081 catgtcattc gctcctttgt gatttctcct atttaataat agcaaaaaaa cggaaagaag
    16141 caatacttat cttctttccg atgcgaccta tcgcgttaaa tattttgtat actcttctgt
    16201 atgaagcaat cgtttagcat tttcgacacg ttcttttgta ggtggttcga tcccttctaa
    16261 tggatatttc aaaccaagtg tttcccactt gtattttccc atcgtatgat aaggaagaat
    16321 ctctactctg tctacatttg acaaggtttg aataaagttg tctagtcgga tcaaatattc
    16381 atcataatca cttcgctcag gaactagtac atggcgaatc caaaccggtt tgttgatttt
    16441 tgacaaatat tgagccattt ctaaaatatt ttcattgtcc caatgcgtta gttttttatg
    16501 ctcttcatta tcgatgtgtt tgatgtcaaa taacagtaaa tctgtatact tcatcaattc
    16561 ttgaaaacgt gaaaagaaag gttcttcata cgtaaaaggt tttccgcatg tatccaatgt
    16621 agtatgcatc ttccgctctt ttgctttttt aaatagttcg atcaaaaaat cgatctgcaa
    16681 tagaggttct ccaccactaa cagtaatacc acctttattt ccccagtaag gtctgaagcg
    16741 ttctgcttta tctaaaagtt catctgctgt gtattctttc cctccgccaa tattccaagt
    16801 atctggattg tgacaaaatt ggcaacgcat gcggcatcct tgcataaaca cgacaaagcg
    16861 cagtccaggt ccgtctactg aaccgaaagt ttcgatagaa tgaacatagc cgattgtttt
    16921 ttcttccatt taagttcaca tcttttcttt gatttcattt taagtacaag ttagctgtcg
    16981 aagcgaagct ttctgaacaa aatttttcgc aattgccgct tcgttatgtg taaaatttac
    17041 agggacggaa catacaagtc ctgcccctgt aaatcgtttc tttcaaataa gcatactgat
    17101 tattcgattt tgatgcgatg gctattcaac gtcctttcga cgctaagtcg aaaaactttt
    17161 ttcacgaact ttttacattc tgtcgtgtga agttctcatc aagacatcta attgttgttc
    17221 tcttgtcaag tcacggaatt taactgcata accagatacc cggatcgtca agttcggata
    17281 tttcattgga ttttccattg catcacgcaa taaatcatta ctgaatacat tgacattcaa
    17341 atgataagct ccttttgaga agtatccatc taagacatta cgcaagtttt cgattcttgt
    17401 atcttgttct tttccaagac ctgaaggatt gattgtttgg gtatttgaaa ttccatccaa
    17461 tgcacagccg taattcattt tagctgttga gttcaggcta gctaataacc cactttgttc
    17521 cgcattgtaa cttggatttg ctcctggcgc aagtggtttt ccagcttcac ggccatcagg
    17581 agtatttcct gtatttttac cgtaaacaac atttgaagta atcgtcaata atgaagttgt
    17641 tggttttgag ttgcggtaag ttttatgacg tttgatttgt cccatgaagt attccaagat
    17701 ccatgaaact aattcatctg cacggtcatc atcatttccg tatttaggga agtcgccatc
    17761 cacttcaaaa tcaactgcta ttcctttttc attacggatt gcttttactt ttgcatattt
    17821 gatcgctgac aagctgtctg ccgcatgaga aatcccagca atacctgtag caaatgtgcg
    17881 ttgcaagtca gaatccatca aagccagctg agctgcttca taagaatatt tatcatgcat
    17941 ataatgaatg atgttcaacg tattgacata tagttctgct aaccaatcca tcatattcat
    18001 gaagttatgt ttcacttctt caaagtctag tatttctgat gtgattggac gatattctgg
    18061 tccaacttgg gcgccagtca cttcatcgac tccgccattg atggcatata atagtgtttt
    18121 agccaagtta gcacgagcac caaagaattg catgtctttt ccgacaactg ttcctgatac
    18181 acagcaagca atcgcacaat catcagaacc ccattgttca cgaagctgat tatcattttc
    18241 gaattgaatc gaagaacttt cgatagcgat tctgctagca tatgttctga atccttctgg
    18301 aaggtgttca gaatacaaaa cagtcaagtt tggttctggt gaaggtccca tatttgttaa
    18361 tgtatgaagc agacggaaat cattctttgt aactaatgaa cgaccatcta atcccatacc
    18421 tgcaatggat aaagtagccc agatagggaa tcctgagaat aattggttat attcaggtgt
    18481 acgcgcgaat ttgaccatac gtaatttcat tactaagtgg tcgatcaatt cttgtgcttc
    18541 tttttctgtg attactccat tatccaaatc acgttggata tagatatcaa ggaatgctga
    18601 gatacgtccg attgacatcg cagcaccatt ttgtgatttg attgctgcta aatatccgaa
    18661 gtatagccat tggacagctt cttttgcatt tgcagctggt ttagaaatat catagccata
    18721 tttcgcagcc atttctttca tttgcgctaa tgcacgatat tgttcagtga tttcttcacg
    18781 taaacgaatc acttcttcac tcattgtatg ataaccagtc atatcatgat cttttttctt
    18841 ttgttccatc aaatagtcga ttccatacaa tgccacacga cgatagtcac cgatgatacg
    18901 tccgcgaccg taagcgtctg gaagccctgt aatgattttg tttttacgag cagcacgcat
    18961 ttcagcagta taagcgtcaa atacaccttg attatgtgtt ttgcgccagt cagtgaaaat
    19021 atgtgtcaat tcatcgtttg gtgtatagcc attacttttt agcgcattgt tagccatatt
    19081 gattccgccg aacggcatga atgcttgttt caaaggtttg tctgtctgta gaccgacgat
    19141 tttttccaaa tctttatcca aatagcctgc atcgtgtgaa gtaatagttg atacgatttc
    19201 tgtgtccata tcatagacac cgttacgctc gaattggata tcatttaact tttgcaattc
    19261 atcccataat ttttctgttg cttctgttgg gccttccaaa aagctgtcat caccgcgata
    19321 ttcagtgtag ttattttgaa taaagtctct agtgtctacg ccttctttcc atttggtacc
    19381 tttaaatcca ttccattgct ccattgtatg gacctcctaa atttctgttt tatgtaacag
    19441 tttttacgtg attagtataa cacaggaccc tatctttgca agggttttct tgttttttat
    19501 ttaacaattt ttatgtttta tttacatata tttaaatcaa cgttgacata ccaacattta
    19561 agcattgtga acactttcat ctttttttgc aaacgtataa ggatttatta aaaatgaatg
    19621 aaaaagagac gccgcctgta ttaatacaga tggcgtctct tcattaaaat actacaacag
    19681 ttacgactcg atgaaaatct caggagcatc gataacttcg atgacttctc cacctttttt
    19741 ctcatccatc gcaaatgatc cattcgatgt tcgatcacct atagggaaat cagttgggtc
    19801 aatcgttaac tgctgccctt tttgattcat caaggttatt tcttttgatc ttccttccga
    19861 catatagata atacggtgcg gatttttttt cagctcacgc atgaccgtca ctccccgctt
    19921 cgcacgtgaa gtttggctga tttcttgagc aagcatgcgt ttaacagctc ctcgctgagt
    19981 aacgattacg atcggcgtat caccttctgc taacacaagc agcccgttga taatcctatc
    20041 tccatctttc aaattcatag acttcactcc agctgccttt gctccaacaa cgggaacatc
    20101 tgttaaagga tagcgcaaac ccattccttg atgcgttact aagaaaacat ctaagcctgg
    20161 ctgatcttct ttcagataca cagcaatcag ttcatcttct tccgccttta gcttcataca
    20221 gctgagcgga cggctcttat aggtgcgcca tggttcgaac tctgtcattc gtgtttgctt
    20281 gatcaatcca ttcttgctga tgaatacaaa tgttttctct gcttgaattt tttgatatgg
    20341 gaaaacagct aaaatcgatt cgtctacaga cagattggag attgtttgag aaatgtgctc
    20401 accagcatct ttccatttca aatcgggtag ttcatggaca gggcggtaga tcacatttgc
    20461 tttatttgtg atcagaagaa catggtccaa tgtactcaat tcacctgagt atagcaagta
    20521 gtcgccttct cgcatcccga tttcttctgg tttggaagca ctataagaac gaaggcttgt
    20581 tcgcttcaca tagccttctc gcgtcacact gacaattacg tcttcttgtg ccactaagac
    20641 ttgcgtatca attttgattt cttcgatttc gtcttcgatt gtcgttagac gagcggaaga
    20701 ataatttttt ttcacttctc gcaattcttt tttcataaca gagaaaagct ctttgtcatt
    20761 tgaaaggatt ttattcaact ctgtgatttg cgcaatcaag ctttcggatt ccttttgaag
    20821 ctctgtgata tccgtgttcg tcaaacggta taattgcaat gtgacaattg cttctgcttg
    20881 ttcttctgta aattgaaaga cgtcgactaa attctttttg gcgtcttttt tgtctttact
    20941 ttcacgaatc gttacgatta cttcatccag aatcgataaa gctttcatca atccttctac
    21001 gatatgctgt cttttttgag ctttggctaa atcgaattga ctacgttttg ttatgacttg
    21061 ctttcgatgc tcgatataac ttcttaaaat gtctttcagt ccaacttgat gcggagtcat
    21121 atggtcgata gcgaccatat tgaaattgta attgatctgt agctcagtgt ttttgaacaa
    21181 ataattcaaa attccttgag cattggcgtc ttttttcaat tcgacgacaa tttgcagacc
    21241 tgttctatca ctttcatctc gtacttcagc tattccatcg attttcttat tcaaacggat
    21301 ctcatccatt tttttcacca aggtcgcttt atttacttca tatgggattt ctgtgatcac
    21361 gatttgctgt ttattaccct tcattgcctc tatctttgtt ttcgaacgaa gaataacttt
    21421 tcctcgccct gtttcataag cttttttgat ttcatctttt ccttgcaaga tccctcctgt
    21481 tggaaagtca ggtccaggaa tatattccat caatttttca aggctcgctt gtggatgatc
    21541 gatcatatag attgtgccat caatcacttc agctaagtta tgagtcggga tttctgttgc
    21601 atatccagca gatatacctg tcgatccatt gacgagaagg tttgggtatc gagctggaag
    21661 gactgttggt tctttttctg tatcatcaaa attccacacg agatcgactg tttctttttc
    21721 gatatctgct agcatctctc cacttaactt ggataatctg gcttcagtat aacgcatcgc
    21781 agcaggtgga tcaccgtcca tactcccgtt attcccgtgc atttcgatca gtacttcccg
    21841 caacttccag tcttggctca tgcggaccat cgcttcataa atactgatgt caccatgagg
    21901 atgataattc cccatgatat ttccgacaga ttttgctgat ttacggaatc ctttgtcata
    21961 cgtattgcca tctttgttca tggcaaataa tatccggcgt tgcactggct tcaatccatc
    22021 acggatatca ggcagtgcac gttcttggat aatatatttg gaataacgtc cgaagcggtc
    22081 acccatcact tcttctaaag ttaattcttg tacttcttgt cgattttcca tcaggtgacc
    22141 tcctattcta cttcaaataa ctgattgttt tcatttttat ctgctcgttc ttcatctaat
    22201 aaatcattag aaactgaagg agagatttca gtatcctctt ttttatctaa gatactgcca
    22261 tcttcttcga gcgtaaattg cacgtgattt tcgatccatt ttcttcgagg ttctactttg
    22321 tcacccatca gtgtggttac tcgacgttca gcttgcgcag catcatcgat acggacgcgg
    22381 atcaacgtac gggaagtcgg atccatcgtt gtttcccaca attgttccgc attcatctct
    22441 ccaagtcctt tatatcgctg cagcatatat cctttgccga cttttttaat cattgctgcc
    22501 aattcgtcat ctgtccacgc atattcaatg acttgcttct ttccttgtcc tttggatact
    22561 ttatataaag gcggtaacgc gatatatact ttcccagctt caatcagcgg tttcatataa
    22621 cgataaaaga aagtaagcag taatacttga atatgggcgc cgtctgtatc cgcatcggtc
    22681 atgatgatta ctttgtcgta attacagtct tcgatagaaa actcaggacc gactcctgca
    22741 ccaatcgtat agatcatcgt attgatttct tcatttttca agatatcttg cattttagct
    22801 ttttcggtat tgatgacttt tcctcgtaaa ggtagaattg cttggaactt tctgtctctt
    22861 ccttgtttag cagatccgcc agcagaatcc ccttcgacta aatacaattc atttttctta
    22921 gggtttcggg attgtgcggg tgtcaatttt cctgaaagaa gagattcgcc ttttttacgc
    22981 ttcttcccat ttcggctttc ttctctggct ttacgtgctg cttctctcgc ttctctggct
    23041 ttgattgctt tacggaccaa catctggctc atttcgctat tttcctgcaa ataaaagccc
    23101 atttgttcac tgatgacgtt atctactacg gtacgagcaa ctggcgtacc aagtttttct
    23161 tttgtttgcc cttcaaattg cagaagattt tcaggtacac ggatagaaag tacagcagct
    23221 aacccttctc tgaaatcact accttccaga ttcttgtccc gttctttcag caaaccaact
    23281 tttctcgcat attcattata agatttcgtc attgccgctt tcattccagc ttcatgggtt
    23341 cccccatctt ttgtccgaac gttattgaca aaagataaga cattttctga atacccgtca
    23401 ttgtattgat aagcgacctc tacttcgata ccttcttttt ctcccgaaaa ataaacaact
    23461 ggtgtcaatg tatctttttc ttcattcaga taatcaacaa attctttgat tccttcttcg
    23521 taatgaaaaa tttccttgac tggtacttct ccacgaagat cagaaagaga gattttaacg
    23581 ccttttagaa gaaaggcaga ttctcttaac cgttcggcta aaatttcata tgaaaatttc
    23641 gttgtactga atattgtatc gtctggtaaa aaatggacag tcgtcccgtt tgcttttttt
    23701 gtttttccta tctttttcaa cgtaccgtca ggtttaccgc cgtttttaaa tttctgctga
    23761 tactctacgc catctctaac gatcgttaca gttagccatt tcgataaggc atttaccaca
    23821 ctggctccta caccgtgcag tccaccagaa gttttgtacc ctccttgacc aaactttccg
    23881 ccagcatgca gcacagtaaa aatcacttca actgttggta ttccggaagc atgcattcct
    23941 acaggcatcc cacgtccgct atctgcaaca gttatactat tgtcttcatg gatggtcaca
    24001 tcaatctcac tgccataacc tgataatgct tcgtcaactg cattatcgac gatttcatag
    24061 actaaatgat gcaagcctcg gctgtcagtg gatccaatgt acatccctgg tctttttcta
    24121 actgcctcta atccttccaa tacttggatg gacgagtcat tgtattcgtt gtttactttt
    24181 tttgccaagg caaacgctcc ttcaataata ctttgcggca aaaccgcacc acgattatca
    24241 tactagaaaa ggtcgtgaaa aaaaagaggg tagacgagaa atcaatttta attatctcct
    24301 aaccgctttt tcgacattcc cctctatcga tcatctcttc tttgtggtca tttttatttt
    24361 tggttttctt tcaacttacc aagttcaatc ttaatacaac gatccatcac gatatctgta
    24421 cgtcctgctt ttcttagcat ctcagctgct tctttgtttt caatccctaa ttgtgcccag
    24481 aacactttag catcagtttt caaaaaatca tctgctactt caggtaaaaa ttcactccgt
    24541 ctaaatacat ctacgatatc gattggatca tcgatttctg tcaaattcgc atagactttc
    24601 tcgcctagaa tttcttcacc agccaataat ggatttactg gtatgatttt atatccattg
    24661 tcttgcaata cctgtgcaac catgtaactt gttcgttctg gtttattgct caagcctacg
    24721 acggcaatat tttttgcttc ttccaaatag ttgaaaatcg tttcttgtgt tggatttttt
    24781 acagacattt ctgccacccc ttgcttttct ttttattcca tctattatac ttgtatcttt
    24841 cattttttca attgttcctg ttacctattc atgctaaaat aaaggatagt tttcaacttg
    24901 aaagagggta aaaaatgaaa ttgatcttat tacttatttt agcatatctt ttaggttcta
    24961 ttccttccgg tgtctggatt ggcaaattgt ttttcaaaaa agatatccga caacacggga
    25021 gcggtaatac tggaacgacc aatacatttc gtgtattagg caaaacagcc gggattgttg
    25081 tcctgcttat ggatatttta aaaggtacat tggctacttg tttgccgatg atcttccacc
    25141 tttctgggat taaccctctt tggtttgggg tatgcgccat tcttggccat acatttccga
    25201 tttttgctgg gttcaaagga ggaaaagctg ttgcgacaag tgcaggaatg cttttaggat
    25261 ttaatcctgt attttttgtt tattcttgta tcatctttat cgttagttta ttttgtacaa
    25321 gtatggtgag tttgagcagt atgatcagtg cagtattgat cactttatct acgatcgtcc
    25381 ttccttatgt agctcctgtg attttggcta agcccaattg gttactgacg atcattgcct
    25441 ttttagtttc ttcctttatc ttttatcggc ataaagacaa tatcaagcgt atcagaaacc
    25501 atactgaaag tcgaatcccg tttggtttga atagcagcaa gaaaaaatcc tgatactttt
    25561 tcattccaaa gagataaaac gggagtgtga caaatcgatg tcacactccc ttattactta
    25621 caggatagct tttttacaca ccttggtaaa cggtgttccc tgctaacttc tcggtcacaa
    25681 cctcttactt cactgtgatg gaatattttg tcttgaagat ttcatgggca cctagttgat
    25741 tgatccctac tttttccatc aagtttccac tactgttcac cgcgtcagcg attccgcacc
    25801 acggttcgat acaaacgaaa ggcgcttctt ttggataagg agaccagatc cctacataag
    25861 gcatatcttt atagcttaaa gttacgctat gggaagattt ttcacttcga atggcatacg
    25921 catttagacc ccttgtttca aaaaccaagg catcttgctc aaaaatttca cgattcaaag
    25981 ccagattcgt atttgtctga cctaacgtct tttgatccat atcgatgaat gggcctttca
    26041 atggtaggcg taatcttgat ttcaatggag aaaatgccag atagaaatct tcaaatgaca
    26101 atccttcttc aagaggtaca ttgaaagcag gatgtccacc gatagaaaag aacatttctt
    26161 tttcaccggt atttgccact tgataatgaa ccgtgatgcc gtctcctcct aattcatagc
    26221 tgatcactaa ttcaaaatca aatggatact tttctaatgt ctccggcgta ctccttaaga
    26281 tgaaactagc catttcttgt ccatgttcaa tgacttcaaa atccatatcc cgagcaaatc
    26341 cgtgttgccc catttgatat gttttccctt gataaacata ctgatcatct tttaaacgtc
    26401 caacaaaagg gaataaaacc ggtgcatgtc tgccccaata cgctggatca ccttgccaga
    26461 tatactctaa accatgttct tttgatttca gactgatcaa ttccgcaccg tgttcagcga
    26521 tttcggcgat cagctggtca ttttgaataa taacagtcat tgccattcct ccaacttcga
    26581 cttacttttt aaaatgggaa ggctggaaca aacatttcta tctgccccag tccccttact
    26641 tctaataact gtgttttgct aactcgtctt taaaacggga attcaatact tttaaataag
    26701 ttcccttcat gcctaacgaa cgagattcaa tgattccagc tgattccaat tttcgtaaag
    26761 cattgacgat cacagaacga gtgatgccga tactatcggc aatattagaa gcagtcagac
    26821 gaccttcatc tccatccaat gcttcaaaga tagcttgtac agccttcaat tcgctataag
    26881 ataatgtgtt gattgccatt tggacagccg ttgcactccg tacatctgct tcgatgcttc
    26941 gagacttctg ataaaggatc tgcataccga caaccgtagc tccatattct gctaagacta
    27001 gatcgtcatc tgcaaacgat tgttccatac gagataagat aatcgtaccc aaacgttcgc
    27061 cagctccaaa gatcggaacg acagtcgtca gaccaaatgg atatttctct cttgactcta
    27121 ccggaaatgc agtaatatca ctagtaattg ggatattagc ttcagttttc atcaactgtg
    27181 ccactgtatc agtataagat tcaggaaatt gtttctcaac aaacattgtt ttgattcgtt
    27241 cattgttcac atcgtgacgt tcattataac ctagtaatac tccgtcactt cctatgatat
    27301 aggtgttgct gtcaagaata ccacctagtg tgacagccat tttatcgtaa ggcaaatctg
    27361 cttgcatgtc aaaagtattc ttttgctgca aaagtgtgtt tatcttgcgg gttttcgtta
    27421 gcaagtctgt cattgtcgtc ctccgatttt ttataaaata tagcgactta aatcttcatt
    27481 tgccgcaatt gagccaagtt tgtcatccac atatgcttct gtgatcgtta tttctcccat
    27541 ttgcatatct ggtgcttcaa ataatagatc ttctagtagt ttttccaaaa tggtatgcag
    27601 tctgcgtgcg ccgatattat ctgtgtcacg gttgacatcg aaagcgattt gagcgatccg
    27661 ttcaattgct tcttttgtaa acgtaactga cacattttct gtaccaatca atgctacata
    27721 ttgttttatt aaagcattat tcggttcagt cagaattttc acaaaatctt cagctgtcaa
    27781 atcatctaat tctacccgta ttgggaaacg cccttggagt tctggaatca aatcactcgg
    27841 tttagataaa tggaacgctc ctgaagcaat gaataagaca tgatctgttt ggatgatccc
    27901 gtattttgta tttacttgtg aaccttcgac gattggcaat atgtctcttt ggacgccttc
    27961 tctggatact tctccagatt gctgtgattt agaagtgatc ttatcgattt catcgataaa
    28021 aataattcca ctttcttccg ctaatttgat tgcctcacta tggatatcag catccttgac
    28081 taattttgct gattcttcct tgatcaataa ctctaaagct tctttgactg ttaccgtccg
    28141 ttccactttt ttcttcggtg tcaaagcacc tagagtttca tttaggtcga tccccatttg
    28201 ctccaatcca ttgttcatca ttggtgcagg tttcttttgg tcttcaactt caatggttac
    28261 ttcacgacta tccagtaaac ccttttgtag ttgttccaag actgtacggc gattgattcg
    28321 aatatcttct gtcacttctt cttgcggttc agcggtattt tgctgactag cattaaaaag
    28381 attcatcatt tgttcaaatt gattagtgga ttgtttctgt tcttttttga taccaggtac
    28441 caagattttt accaaacgtt tttctgcatt ttttttagca cttgtgtata cacggctgta
    28501 ctgctctttt tctacgattt ggatcgcatt ttctactaaa tcccgaacca ttgattctac
    28561 atctcttccg acataaccga cttcagtaaa ttttgttgct tcgactttga caaatggtgc
    28621 attgacgatt tttgccaaac gtcgtgcgat ttctgtttta ccgacccctg taggtccgat
    28681 catcagcata ttttttggtg taatatcttg ttgcattttt tcatctaact gcaaacggcg
    28741 atagcggtta cgcaaagcaa cggcaacaga tttttttgct gcgtgctgtc ctacgatata
    28801 ttcatctaat tcacttacga tttgtcttgg tgtttgtttt aattcataca tatctactca
    28861 cctcttacag ttcttctacg ataatattat gattagtaaa gatacagata tctgcggcaa
    28921 tatttaacgc attttctgca atttctttcg cactcatgtc gttttgagca tagtttttca
    28981 ttgctcgggc tgcagccaac gcaaaattcc ctcctgatcc gatagctaag ataccatcat
    29041 ctggtgtgat aacttctcct gtacctgaaa caagaagcat ttctttatca ttcattacga
    29101 tcagcatcgc ttcaagtttt tgcatagatt gctgtgtccg ccattcttga gctaattcga
    29161 cagctgcgcg ttgtagattc ccgttatatt cgtttagttt gccttcaaat ttttcttcta
    29221 aagtaaacgc atctgcaaca cttccggcga aacctacaac aacttctcca ttgtagatac
    29281 gtcgtacttt ctttgcagtg cctttcatga caacagattc acccattgtt acttggccat
    29341 ctccagccat agcaaatttc ccgtcttttt cgacggcaca gatagtggtt gaatgaaatg
    29401 gtgattcgac catgtttgtt tcctcctaaa tttgttcttt tccttatttt tacgctcttg
    29461 gatggaaaga acgatagttt ttttgtaaac tttcttttgt tacatgggca tagatctggg
    29521 tagtcgataa attggcatgt cctagcaatt cttggactgt ccgcaaatct gccccattat
    29581 ttaataaatg agtagcaaat gtatggcgca gcatgtgagg atggatatca ctatccaaac
    29641 tgcttttttt gatcatttga ttcaagacat attcaattcc agtaggcgtg attggttttc
    29701 catgatgatt cacaaaaacg atatcatgct cttcgtgata ttttgtcatc aatacctggc
    29761 ggccttccct gaaatattct tgcagagcat cttgagcaaa agaacctaga ggagcatatc
    29821 tctctttgtt ccctttacca tggatcaaca tgacctctga atcaaaatca atgtctgata
    29881 ccgacaaact gctacactca cttaaacgga tgcctgaccc gtaaaggact tccagtaaag
    29941 cgcggttcct caaatccaaa ggactatctc ctgctacgct gtcaaaaagt gcttgcattt
    30001 cattttcata aaaaaaacgt ggcagtctca aatttttctt tttgagatga acgtaagaaa
    30061 atggattttc ttttatcact tcttctttga gaagatactg ataaaacgaa cgtaggctgg
    30121 ctatcttgcg actgatagaa tttctgctat agctaggacg atgatcagct aatgcactca
    30181 aatagacacg aaccgtaaaa tattccgcct tcagcagatc agcgtctcca gattcctcca
    30241 aaaaatgttt gaaatggaga atgtcttctt catatgcttc tttcgttttt tctgaatatc
    30301 ctctttcgac aatcaaataa cgcaaaaatt tttgttccca cgtggcttct gtcattttct
    30361 caccttcctt ttgtcccaga gaataattta gcacagcgtt tataaaaaga caaataaatt
    30421 gtcagaattt aaccaatatt tctaagtttg ttcacaattg gcgcaattcc tcacattttg
    30481 tttgagaaat tcttaagaaa aacgcaagaa aaagagcgag actatccgtc tcaccctctt
    30541 catggagttt ccttactcat ttgggattgc agttttttct aattctgttt ttattgcctc
    30601 attagctttt aaagctcgtt gtgcaattgc ttcatatctt gcttttttgt ctcgaatgcg
    30661 ctctggcagt tcaggaaaca gaccatagtt ggcattcatc ggttggaaat gttttccttc
    30721 cgcatgagta atgtagtatg ccatacttcc aatagctgtt tcttgtggaa actcgactgg
    30781 ttccatttct tttgctaagc gtgcggcgtt gattccagca agtagtccgc tcgctgcact
    30841 ttcaacatag ccttctacac cagtcatctg acctgcaaag aacagatctt cgcgtttcgt
    30901 tgattggtaa gtcggtttca gcaattctgg cgaattcata aagctgtttc ggtgcatcac
    30961 accatagcga acgaattcag cattttctaa cccaggaatc atccggaaaa cacgtttttg
    31021 ctcgccccat ttcaagtgag tctgaaagcc cacgatgttg tataaagaag ctgcagcatt
    31081 gtcttgacgc agctggataa cagcataagg acgtttgcca gttttaggat cttccaatcc
    31141 aactggtttc ataggtccga aaagcatcgt tttgaatcct cgctgtgcca ttacttcaat
    31201 tggcatacat ccttcaaaat atttttcttt ctcaaaagat ttaagttctg ccacttcggc
    31261 attgattagt gcctcatgga aagctttgaa ttcctcttct gtcatcggac agttcaagta
    31321 agctgcttct cctttgtcat agcgggactt caaatatact ttatccatat cgatcgtagc
    31381 tttgtctacg attggtgcag ctgcatcata gaaataaaaa ccatctgaac cattgaatgc
    31441 tttgatgctt tctgctaatg gatgagaagt caaaggtcct gttgctacta cggtgattcc
    31501 ttccggtatt tcagtaattt cttcgtttat cactgttact aatggatgct ctttgacttt
    31561 tcgggtaatt gtttcagaaa aagaatcgcg gtcaacagct aaggcaccac cagctggcac
    31621 tgctgtttca tctgccgaag tgattactac cgaatccaat cggcgcattt cttctttcaa
    31681 taaccctact gcattagcca aactgtttcc tcttaatgaa ttggaacata ctagttcagc
    31741 aaaattactt gtgtgatgcg cctcagtcga tttgactgga cgcatttcat ataaacggac
    31801 agggactcct gcttgtgcga tttgccaagc agcttcactt ccggcaagcc ctgctccaac
    31861 aacatttacc attttcaatg aaagaacctc ttttctatta tttttggatg tcttcttcgt
    31921 aatctccatt tacacagact acttgtttac cgcctttgac tttcttctcg accagatatc
    31981 cgtcacattt tggacaattt ctgcccactg gtttatccca agaagtgaat tcacattctg
    32041 ggaaacggga acaaccatag aatagtctgt ttttcttaga ttttcgttca atgacttgtc
    32101 cttcatgaca tactgggcaa gtcacaccga tttcttttac gattggtttc gtattccgac
    32161 aatcaggaaa attactgcaa gcataaaact tcccatattt ccctaatttt attaccatcg
    32221 gatgtccaca gacatcacaa tcgaatcctg ctggttcatc tttgatttga attttttcga
    32281 ttttttcttc cgcattcgtc agttcttttt caaacggttt atagaaacga tcaaccactt
    32341 tgacccacgc ttccgtgcct tcttcaattt tatccaaatc tccttccatt tctgctgtga
    32401 aatggatatc gacgatttga gggaagaatt cacagatcag tgaattgaca atttctccta
    32461 gttcagtcgg ctcaaatctt ttttgtgtca attttacata gtagcgtctt tggatcgtat
    32521 caagtgttgg tgcataagtg gaaggtcgcc ctaccccgtt ttcttctagt gcacgaatca
    32581 acgtagcctc actaaaacgt gctggtggtt gagtaaaatg ttgttttggt tcaatatcaa
    32641 cagattggac tatatcgcct tcttcaagtt ctggaagaat attttctttc tcttctttgc
    32701 cgtcatctct accttccaca tacacttgca tgaatccttt gaatttgatt tttgaaccgt
    32761 tcgcaatgaa gatgacacca ttttgttcta atgtcacttt cattgtatct aaaatagctg
    32821 gtgtcatctg actagccaca aaacgtgacc agatcaatgt ataaagtttc aattgatctt
    32881 tatctaaata ttgtttgagt tcatctggtg tacgaagcac actcgttgga cgcacagctt
    32941 catgggcatc ttgtgcgcct tgagcatttt tgatctttcg gtgatcatgt ctagaaaatt
    33001 cttttccata cgtatcttca ataaatgctg cggcctctgc ttttgctgta tcggcaatcc
    33061 gggtagaatc agtacgcatg tatgtaatca agccgactgt tccttgttta cccaaagtga
    33121 ttccttcata taattgctgt gctaccatca tggtttttct tgttcgaaag tttaacttac
    33181 gggcagcttc ttgttgcagg ctacttgtag tgaaaggatt cgcagggttt cttttgcgct
    33241 ctttcttttc tactttttta acttcgaagt ctgttccatc aattctagca gtgacttctt
    33301 ttacactttg tgcatctggt aatttctttt tcttaccgtc gattccccag aaatttgctt
    33361 tgaatttctt gcgagctttt ttaaaattac cgtcgatgct ccaatattct tctggaacaa
    33421 attctcggat ctctttttca cggtcaataa taatttttaa agcgacagat tggacacgtc
    33481 ctgcacttaa ccctttcttg accttacgcc agagaatagg actgatcgaa taaccaacga
    33541 ttctgtctaa ggtacgacga gcttgctgtg catctactaa atctacatcg atcgttcttg
    33601 gttccttaaa agctgctttg actgcgtctt ttgtgatttc attgaagacc acccgatttt
    33661 tttctttcag atccaaccca agtaggtacg ctaaatgcca agcaatcgct tctccttctc
    33721 tatccgggtc acttgccaag taaacttttt gagctttttt tgctgcggct ttaaggcttt
    33781 tgatgacatc gccttttccg cgtatagaaa tataatgggg ttcataattg ttttcaaaat
    33841 cgatacccat tttacttttc ggtaaatctc ttatatggcc aacactggcc attactttat
    33901 agtttcgtcc aagatatttc tcaattgtct tggcttttgc aggtgattct acaatcacta
    33961 aatatttata cgccattact gaatggactc ctttccattg tcgtgttcct actattaaaa
    34021 gaggattcat acaaatcgta gctaatcata tccattcaga aaacgtttgt caagaaaagc
    34081 aaatgaaaag tatggtgtta ttctttaaat tctgccaaga tatcttcacc cgaaaccgtg
    34141 caaactgcac cgtcttggat caaatgatga catccggtag aatgttctgt taaaatattt
    34201 cctggaacag aaaaaactgt tcttccaaga tccaatgctt gctgtgctgt tatcagagag
    34261 ccgctttttt tcttggcttc aaagacacat gttcctttag acaaagctgc gataatccga
    34321 tttctcattg gaaaacgatg ctttttcggg ccggattgag gaggatattc actcaaaaga
    34381 agatgatcat ttgcaatcac ctcttgcaag tcacgatttt cagcaggata gacttgctct
    34441 atccctgttc caatgactgc gattgtccgt ccaccatgag agattgctgc ttgttgaccg
    34501 tatgtatcta tccctttcgc gcagccactg acgatcacat atccagcttc taccactggt
    34561 tttagtaaat aataaactgt ttttttggct aaagcagaag tgtgtcttcc accaacaacg
    34621 cttaacatgt tacaagaaag taaagataga tcaccattat aaaacagtac aattggcgga
    34681 taggtcaagt gaagtagctg agggggatat ttgggatcaa agcaagtgat aaacgactgt
    34741 ttcgattgga ttttgcccca gttagcgtga atttcctgcc aacttttgcg aaacagctcc
    34801 cgatgtgtgg ttattttacc tatcttaatg atgtcttcaa atccgatttc tttataagga
    34861 tactgcatca tccagttagc aagtgcccat tttccttgac tactgattcc ttttgcataa
    34921 gcaagtttca ataaattctc ttctatttga tacatcataa aaccgccttt cgtctctctt
    34981 tctaataaga tacgtaaaag cggctgtttt ttattgtaaa tattttttta caggtgcaaa
    35041 acttttacga tggattggcg taattccttg tcgttcaagg ccttctaagt gttcctttgt
    35101 tccataacct gcattatgcg agaaaccata tccaggataa agcacgtcat attctttcat
    35161 caattggtct cgatatactt tagcaatgat acttgcagca gcaatcgaca cagatcgtgc
    35221 atcccctttg attatttttt cctgctggat aggcaaatcc aaggtcatcg catcgatcaa
    35281 caaatagctt ggtacaatgg acaatttttc tactgcttgg atcatcgcta atttacttgc
    35341 ttcataaata ttcacatcat cgattgtctg ctgatcaacg atcccgatcc cgattgccag
    35401 ggctttttct tgaacttgtt gataaagaag ttctcgcttt ttctctgata attgtttgga
    35461 gtcattcagc cctaatatga tctcattttt cggcaagata actgcagctg ccacgacagg
    35521 tccagctaat ggtcctcttc ctacttcatc aatgccggca atccattggt gaccttgtgc
    35581 gtacccttgc ttttcaaagc aaaacatttc ttcatatttt tccttaatta atgcttcctt
    35641 tttcaatcgt cgttcaaaag aagccagtgc ttgctgcacg cctttgcgtg gatcatttct
    35701 taatgattct agtgtaggat catctattct ctgtatgctt ttcaatcgtt ctttgatact
    35761 ttggatggat tcagtcatca cttttttgct ccatttcttc gcaacgatcc agtgtgtatc
    35821 gtccaagttt tccttggcga atatcatgca cgactaattc gctgccgcga tcatagtctt
    35881 ctttataccc acgttttgct gtaatatcca tcagtaattc cggtgccaat ttttcttctt
    35941 gctcgattga taaaccataa cgatcgctga ttcttcctga ataatatcgg gcaaaaaagt
    36001 ctaatccgta aatcgctaaa tcatctaaat ggacaagctg gtctttgatc gcgcctgtca
    36061 gcgctagttt tttgccgatt tcttcatctt caaatttggg ccataaaatc cctggtgtgt
    36121 ctaacaattc caactcatta cctaaacgaa gccactgctg accttttgtt acgccaggct
    36181 tatttcctgt ctgtgcaatt ttcttgccta ctagtcgatt catcaacgta gatttcccaa
    36241 catttggaat acctaagacc attgcccgaa tagcgcgagg cttgatcccc cgagcagcat
    36301 ctcttgcccg tttttctttc aatgcttctt tagccgcagg tactagacct ttaattcctt
    36361 tactttcttg tgccgtgata gctaatgcaa gatggccttg tttacgaaaa tattcttgcc
    36421 acagctttgt ctgttgtgga tctgccagat ccgctttatt caataaaaca aggcgaggtt
    36481 tttgctgaat gatctgatct agcattggat tacgagaaga taaaggtaat cgtgcgtcta
    36541 ccaattcaaa tacgatgtcc acatatttca ttttttcaga aacttctcgt cgggcttttg
    36601 ccatatgtcc cggaaaccat tggatggtca ttgttctctc cattcctttc ataccggaat
    36661 attccccggc tttacttgtt ttttttcttt ttcaatataa attcactttt ttattataaa
    36721 gaaattccat ctattagtta agtgaaagtg aaccagtcct cattctatca catcttcact
    36781 tttttgatga tgtaaatttt tattttggtt tctatatatg aatctaaatt tttgacaacg
    36841 tttcctaaat aagagatgat agacgaacaa aacagaaagg agacgaaaca aatgaatagt
    36901 atggtttttg ttaattttcc tgttgctgat gtcaatcgtt ccgttgcttt ttatgaaaag
    36961 ctgggtttta aaaaaaatga agaattttct actgaagaag ctggttcaat ggtatgggat
    37021 gacacttttt gggtaatgct tctctctcat gatttttata aaaaatttat aaaagataaa
    37081 gaaatagctg ataataaaaa aacaagtggt gctttgattt cgtttagtgt tgaaaacgcg
    37141 gatgaagtaa aaaaatttgc tcaaattgca aaagaaaatg gtggtacttt ccacgaagta
    37201 gatatgggaa tgcctgaaga agaaatgttt tcattagaag tccaagatct agacggaaat
    37261 acattagaac ccgtatggat gaagatgtaa gcagataact aatctgacgt tttttataac
    37321 aaaaaaacaa aggcggatga tacgataagt attctccgcc tctatttttt tcattcgtac
    37381 aatctctttt ttacagttta taaaagcagt cttctcaccc tttttttaat tgttagatgc
    37441 cagtgtatgt aaatcaccaa tgtgtcatga cacattggtg attttatggt atccttttat
    37501 tattaactga caaaggagag aatattttga acactttgat tgcaaaagac gtttttagat
    37561 tgcccgaaat gaatgcaaga ttgttgtcga cctctgctct tttattagcc cttgatgtca
    37621 tcttatacaa gatagcgatt ggtcctgctt attttcaaat cacattcgga tttttaagta
    37681 tggcgcttat gggttatcta tatggaccga tctgggctgg tttgcttgaa atgttgtcga
    37741 acactttaaa tttcacgata tttggttcag gaacgtttca agctgctttt cttatcacgg
    37801 ctttccttgg tggatttatt aatggcttat ttttatatca taaaaagata aacctgcctt
    37861 ccgtcatcgc tgcccaatta ttgattatga tcccagtcag cctttttatc aattcttggc
    37921 tgatgtctgt tttgtttggt gcagatttca aagtgatttt tctaggtaga gtcatcagga
    37981 atatgatcca aatccctatt caaataatgg ttgtttattc atttatcaaa actttagagg
    38041 ctagaaaagt gtttaaacga gctgtttgat tttacgtact aaggtagtaa actgattttg
    38101 gattaaacaa taaagcgtgc agtctcaaca gattgcacgc tttattgttt ctcactggat
    38161 ccattttatt tcatcaagtg gataatagac aaatcttgct ttacctagaa tatcagtttt
    38221 attgatcgtg ccaaacgaac gactatcttt tgaaactctt cgattatctc caagtacaaa
    38281 ataattatct tccggaagct tggattgtcc cgttagctct tgtagtgtaa agtccgtcgt
    38341 ataggatgca tttgcatgat ctgatttcag gtttttagtt agataaggtt ccttaactac
    38401 tttcccattg atcttcaatt gatcattttg gtagctgaca ttttctcctg gaagaccgat
    38461 tactcgtttg atataaatcg tgccatctgc taattgaaaa acaacaatat caaatcgtct
    38521 gatctccgaa aatttttcca tcacaaccat atcaccttgc ttcaacacgt tttccattga
    38581 attgccttcc actggtaccg ggatgaataa aaatccccgc aacatgaacg caaggaatac
    38641 agaaacaagt aagtatttga atacaagcca gaagcgatta atatagcgtt gtttttttgt
    38701 caatctctat caatccatcc ctcgtttttt cattctttct ttattataag aaaaatatca
    38761 aaaaaatgcg actagatagc tgaaataaaa ataattcttt caattaggct attggacaaa
    38821 aaactttttt gatcataaag tacaaaacta tctcttatat gtcatgatta ttttctaaat
    38881 atttttccca tgcttcgtca aagatatcca tggacggcaa atagtcgaca ttcagttcca
    38941 gataattaga aatttcttcg tagctagagc tgtgcttagg aaattggatg tctttaccgg
    39001 cttctgtagc gaaaacagat aaagcatcat gtgtgccacc gcctcgcagt gtcattaaat
    39061 aatgaaaaaa gcttcgattc acccttcgtt cctccaattt tcttcgataa aggcagcccg
    39121 gcgtgcatgg tttcttttaa aattttcagg atgcttttta taatactctt gatggtcttc
    39181 ttctgctaga taaaatggtt gggctggttc gatttttgta acgattggct gttcgaaacg
    39241 tccactttca gctaactgtt tcttactttt ttcagcaatg atccgctgtt cttctgtcaa
    39301 ataaaagatc accgggcgat aattgtcacc gcgatcctcg aattgtccga aagcgtcagt
    39361 tggatcagtt tgctgccagt aaagttctac taattgttca tatgaaatca atgttggatc
    39421 atagataatc tccactgctt ctgtatgtcc agtcgtttgg ctttttactt gttcataagt
    39481 cggatctggt acatgccctc cagtatatcc tgacatcact gtatggatac caggaagcgt
    39541 gtcgaaaggc tggatcatac accagaaaca gcctcctgca aatattgctc gttccattat
    39601 gattccccct ctaatactac tcatccgatg attttttatt ttcttttgtc ttgctttcag
    39661 tactgctggt gctagttttg ttggtatttg agttgtttga agcatcagag gtatcttttg
    39721 gcaaatatag actaaaccga atgtcatcat ttactaagtc gattttatct gctttaatat
    39781 acatcccgtt ttgcattcgg aattgatcca acctaagcat gatagtcaga tcatctgtat
    39841 tgacttcgat ccaatctggt aatttatagt ttctcttgat catattcatc acatctttga
    39901 ttggcaaact caatgttccg atagaaagac ttctagcttt tagttggaca tttccgtttt
    39961 ccatcacata cggatcaaaa tataaataga aatcgatagg aaaacctagt acttcgaaag
    40021 taccattgag tagtgcttca ttttccaaat aaaatttata ttcaatatca ctatcttttt
    40081 gatactcact aaggaaaaag ctgatgattt gattgacttt ttctttattt gaattgatcg
    40141 ttaagacagg atctccttgt ctttccgtaa gagctggtat ttctgggagt tctggttccc
    40201 gattagcaaa aatacggctg cctaaaaata cggtcgaacc aagtactacc ccaagaagga
    40261 taaggaaggc gaatttccag taatttgttt tctttgtttt gtttgaagat acgtcagatc
    40321 ttgttttcat gtttattgat catcctcctt ctctagccat tggttttgtg tctcgatcag
    40381 ctgatctctc actgcatttg ccatcaattg ataacctaaa ttatttggat ggaaatgatc
    40441 ttcttcatag agcacattat ttttgatgcc atttagattt tctgtcgtgc tagttgtttg
    40501 atctgttcct tcttctgtga cacctacttc ctgattcaat ccttgataaa gcaaatcatt
    40561 gattgggata aagtagcagt tcggcatttt ttttgtcacc gtttctgttc ctttgttcca
    40621 attgttaaca attgtctgca tgtcagtgat ctcaggaaag ttcaaataaa atggattgta
    40681 gatgcctaaa atgtaaatag gtgcggtttt attgagggat cgaaattctt ccaatagttc
    40741 tgtgacattt tcctgatagt ttttcagtgg ctttttaaag gaattgaccg ttaacccgaa
    40801 taaattattt tggataactt tcatcaaatc attaccgcca acagtcagtg tgataaaatc
    40861 agctgttttg atattgtttc gaatttcttc ttttgttttt actcgcttta agatctggtc
    40921 acttcgttca ccatttacac catagttttc tgtttcaacc gcggtcaaat tataacggtc
    40981 ttttagatca tttgcgacaa ggggaacgaa tcctccttgc ttggtttgat ctcctactcc
    41041 ttcagtcagc gaatctccga tagctatgta gtgaagtttt tcttttttat ttggatcttc
    41101 tctcacagta gttactggtt tcattagtgg gccagctttc ggtatagaag cagctaaacc
    41161 tgcatagatg atgatcgcaa ttaataatgg gaacaaaacg aggatcagat gtcgtttcgt
    41221 taatttcatt ttttcacctc ttgtattcga ttggttctaa tgttatcaaa aattttatca
    41281 aaaaggtagt ctttatgaaa aaagaaaaca attattttcc taattttata aagaaaaaga
    41341 gaataagctg caagcctttc aggctgaggg cttatcctct tttcgactaa tctacataat
    41401 acatgattgc aaacgcgtct ttaccagtat gcgtagcgat aaccggattc gtatgcagta
    41461 cagggatatg catgtccggg aataattctt gcaaaccctc tttgaattct tgcgccaatt
    41521 cttttgcatc cgcatgtgaa ataccgattt gtcgcacatt tggcaattct ttcagttcat
    41581 ttttcaaatt attaaaccat ttatcgaacg ttttctttcc tcgcccctta gttacaggga
    41641 ttaattcaga atgggcgaaa ttcatcacca cgcgtatgtt caaaacacta gacagcaaac
    41701 cagttgttcg actgattctt ccacctttta ccaagttatc tagtgtggat attccgatgt
    41761 ataattttgt attgtcttgt acttttttga cttcttcaag gatttcctct actgttttac
    41821 cttcttttgc taattcagct gctcgaatta cctgaaaaga taatccttgg tcagtggtat
    41881 cactgtcgat aaccgttact ttcgtttttg ttagattact ggcttgtctt gctgcttcta
    41941 ctgttccact caagcctttt gtcatatgga tcgaaatcac ttcactgccg tcttctccta
    42001 gctcatcata catcgagata aattctccaa tcggtggttg gcttgttttg ggcagatttt
    42061 ttgcttttgc catcatttcc ataaatcttt cgccgtctag ttggtcatca tcgggatata
    42121 ctgttccgtc gatcatgatg gatagcggca tgacacgaat gcctaaagca tcgcgtacat
    42181 ttttttccat cgtacatgaa gaatctgtta cgatttttac atttgtcatt attttctctc
    42241 tctctttctt taacaagatg ccttattcat gttaaactac atgtaagaca atttctattt
    42301 ttagtataac agacaaataa tgatttttca ttaattacac ctagtaaagg agccgttttc
    42361 atggaaaaaa cgaaatttaa tcgaacgtat gaaatattaa atgaagtgtt taatgcagtc
    42421 acccatggaa ttgggactgg attgagtatc gcgggactag tggttctttt agtaaaaggt
    42481 gcccacttgc attcctccct ccatattgtc tcttatgcta tctatggatc gatgatgatt
    42541 cttttatttt taacgtctac tttgtttcat tcattgattt ttacaaaagc aaaaaaggtg
    42601 ttccaagtat tcgatcatag ttcgattttt cttttgattg caggaagtta tactcctttt
    42661 tgtttgctga gtatccgagg atggctaggt tggactttat ttgtattaat ctggttgttt
    42721 gcgattcttg gcattatata taaagcgttg acgttgcata aacgggagag agtatcaaaa
    42781 gtatctacga tcatctatat ttttatgggg tggctctgtg tcgtggctgc tgttcctctt
    42841 tatcactctc ttggaatgac tggtgttatt ttgatggtag caggcgggct ttcttacacg
    42901 ataggcgcat tcttttatag cttgaattct atccgctata tgcatgttgt ttggcatttg
    42961 tttgttatgt taggtgcagg ttttatgtac ttctctgttt tattctttac ttaataaaaa
    43021 aaaataaagc tatggatggg acagctctaa ataacagcta gtctatccat agctttttta
    43081 tatcgtcaat tcgcctgtcc cgctttttac aggattctca ttacgctgat acgtttcaaa
    43141 ttgatgcgga taacgatttt tttcatctat ttctccttga ctagtattga tcagtgacca
    43201 atcagaccag tcaacaggtg gaaaataagc atctccatcg aatacatgat ggatgactgt
    43261 acggtataat atcgtacaaa atggcagaaa ttcttgataa acagctgacc ctccagcgat
    43321 gaaaaagata ccgtctgttt ctttttccag agcaagcacg tcttccacac tatgcatgac
    43381 atgcacgcct tctgctttat aggaagaatc tctcgtcaaa acaatcgtct gacgattcgg
    43441 caacggacgg ctacccatcc cttcgaaagt ttttcgtccc atcactagtg tatttgcttc
    43501 tgtcatttgt ttgaagaatt tcagatcatt tggcaaacgc caaggaagac ggtcttcttt
    43561 tccaatcaat ccgttttcat cctgtgccca aattgcagct aacattttct catcctttct
    43621 gattctaaac agcgatagga gctttgatcg ccggatgtgg gtcatatcct tctaattgga
    43681 tatcttccat ctcaaaatca aatactgact gcttttcctg attcaaaact aaagtcggaa
    43741 atgcgcgtat ttctcggctc aattgttctt tcatttgttc gatatgattg atataaagat
    43801 gagcgtctcc taatgtgtga acaaattcgc caaccgctaa acctgtttca tgtgcaatta
    43861 ggtgagtcag caacgcataa cttgcaatgt tgaacggaac gcctagaaac acatccccac
    43921 tgcgttgata caattggcag cttagccgtc cctcttgaac ataaaattga aacatcgtat
    43981 gacaaggagg caaagccatg ctaggaacat cttccggatt ccatgcagaa acgatcaatc
    44041 ttctagaatc aggagtcttt ttgattgctt cgatcacttc tttgatttga tcgataaagc
    44101 caccgtcttt tgtttcccag tgacgccatt gatagccgta gatgtgtcca agatcgccgt
    44161 atgtttcagc aaaggaatca tcggttagga tcttctcgca aaaaagcttg ctctgctttt
    44221 tatattcttc attgaattca ggatctgtca aacatctatg gccaaaatca gtcatatctg
    44281 gtccattata gtctggactt ttgatgtagc gttcaaatgc ccattcatcc caaatatggt
    44341 ttttattttg taatagatag cggatattcg tatctccttt taaaaaccaa agcaattcgc
    44401 ttttgatcaa tccaaaaggt actcgtttcg ttgttaacaa aggaaaccct ttttgcagat
    44461 caaaccgcat ttggtatccg aataagctta atgtgcctgt gcctgttctg tcttctttac
    44521 gatgcccttc ttccaacaat tttcttccta agtcaagata cgcttgttcc atcctttttc
    44581 ctcctaatct tctacccatt cactcaagta ttcccaccgt gccatttttt cttcaagttg
    44641 tgcttctgtt tcagatacgt cgttttgcaa ttcttgtaat cgagtgaaat tatctccttg
    44701 atgattcatc tcttcttgga gcaatgagat tttttcttca agttctgcaa tctcatcttc
    44761 aatcgtttcc cattcttttt gttccatgta agataatttc ttcttttcag caggttcctt
    44821 gctctgtatt tttggtttcg ctttttcagc aggctgctgg gtctcttttt gcttcagtaa
    44881 ataactggac atgtttccgt aataaagttc ttgttttccg tttccttgga atactaataa
    44941 cttatctgct accttgtcca agaaataacg gtcatgggaa acagtgatca cagcacctgg
    45001 aaaacttcta aaataatctt ccaaaatcgt caatgttgct atgtccaaat cattggtcgg
    45061 ttcatccaat aataaaacat taggttgctg gatcagcagt ttcaacaaaa ataaccggcg
    45121 tttttctccg cctgaaagtt tgcctatcag tgtcccatgc ataaatcttg gaaataaaaa
    45181 tcgttcaagt aattctgcta ctccgatttg actgccatct gctgtttttg cttcttcagc
    45241 agcttcttgt aagtaagcaa tcatccgttt atctggtgcc atttcttcat tttcttgtgt
    45301 ataatacgca agatgaaccg tttcaccgat agacattgta ccgctatcca ggggaattct
    45361 gccagctaga atattaagca atgtcgattt tcccgcacca tttttaccag taatacctag
    45421 tctttcccga gattgaatca gtaaatctaa atgttctagc agtgtttggt catttatacg
    45481 gtaactgcca tctttgattt ccagtacttt tttccctaaa cgttgtgtcg ctaggttcag
    45541 ctctaaatca tcttcttggt ttacttggta caagttctcc tttagatctt cgaaacggtt
    45601 gatccgtgct tgctgcttgg tgccacgtgc ctgtacgcca gccctcatcc aagctaattc
    45661 ctgtttataa agccgcttgc gcttttcttc ttgttctact tctacacgat cacgttctgc
    45721 tttttctaag acgtaagctt cataatttcc tttatactcg tataattttc caaatgaaag
    45781 ttcgaagatt cggtttgcta ctctatctag aaaataacga tcgtgggtaa ccatcaatag
    45841 tgcgccacgg tattgtttca aatagttttc caaccactcg atcgcttcat aatctaaatg
    45901 gtttgttggt tcgtctagta atagtaaatc gggttcatca atcaacactt gcgccagact
    45961 aactcgtttt ttctgaccac ctgataaagt actgattttt ttatctaatt cactgattcc
    46021 taatttttgc aaaataatct ttgcgttggt atctgtagtc caagcatctt ctttattcat
    46081 ttgttcttct gctttagcat aacgtttttg cacatcaggg tcttctccgt tttcccctag
    46141 ttcgatcaat gcttcctcat atgctcgaac agtttggatc aaggggctgt ttccttgaaa
    46201 gactgcttgg agaacagtta attcatcgga aaattcagct gcttgagaaa gataaccaat
    46261 acggtaatca gttggataaa acactgtttg ccgatcaccg tcgccgctat cgataccggc
    46321 aataatattt aagagacttg ttttacccgt cccgttcgtc ccaatcaacc cgatacggtc
    46381 tttttcgtgg accaaaaaag aaatctggtc aaacaaatct ttttcaccat aagttttttt
    46441 gaggtcagtt acttttaatt ctttcaacat gttccctcct tatcttggat attgtatcat
    46501 aaaaatagtg aacttcagga aaattttttt ggtatttgtt ctttttgtgt tctcaaaaaa
    46561 agaacaggac cgattcgatc ctgttctgat atgttttgtt ttttataaaa cttgaaaatt
    46621 tttacctttt tattttattg gactcgtttt tttgactttg ctcgaaacgc aaatcggaaa
    46681 gaaaagttat cagatggttt gttcatcgta taagattcta acaaattgat attcctgcga
    46741 tagctgttcg cagaaaacgt cgtgattttc tcagcaactt cgtattcatc aatgattttt
    46801 ctttcaagat aataacctct tagcattttt tttgtaaaca gactatcttg ctggatcatc
    46861 cacgaagaaa tcaaattccc cgagttcatt tttattcctt caagtttgcg ttgctggata
    46921 aaaaattgaa ccatcgtttc atcataaata tcagtcaagt tatctaaact ttcaataatc
    46981 aactgagtat ttttttgata tgtctctctg atttcttgga tatcttcatc actaataaat
    47041 gtctgacgat cagcatgtct tctttgccaa aacattttcg gatggagaac gattcgcatt
    47101 aacctacgga caacaatgaa ccaaaagccg ataaaagaca aaatctggct tgtgatagaa
    47161 tgttcgaatc gagaaagaaa acgggagtaa aaacgataac cattagaaga aagcttgccc
    47221 gaacgataac tttcatccaa tccatcccgt tcgatcgaca aaatcagtgc ttgtatttct
    47281 tgtacttctt gcttttcatc atcggttaaa tcttccaagt acagatcacg tatccgttct
    47341 tgataatttt ctatcactgc ttcagtggct aaaaattctt ttgtattacg atcaatttcc
    47401 tttatttctt ttcgcaaaac ttcaacgacc tcttctaaaa tcatcaaagc attcaaatcc
    47461 gtgcttttcg cttcctctgt ttccgtcagc ataggcaaga caatgatccc tatgaccaaa
    47521 gtcactaaga tgacacccgc cgtcagaaac aaaagcaaag accgctggta aaacatcata
    47581 ttattgattg aaaatggcaa aataaaaatc gtagccaaac tgactgttcc ctttacaccg
    47641 ccaaatgtta agatcaagat ctcgttcaac tgttttttga actttttact gccatctttg
    47701 aatacataaa aaagactgat agaaatgaat ctgatcacaa aaagcatgac agagattaat
    47761 acgataatcg ctaataaaag accgtttgga tataaaccgt ctccccatac tggcgagaac
    47821 acttgcgtca attcgattcc taaaaatata aatactaatg cattcagtgt aaaagcaata
    47881 gttgtccatg tactgtggga caagctcgac aattctgcat caaacacaga gattttccta
    47941 aaaccagaag cttggagaat acctgctgcc actgcagcaa tgattccaga tacatcaaat
    48001 gcctctgcca atacataggc tagaaaagga agcaaaagtt cgatcagcag ataccctgtg
    48061 acatcttgtg ctgatgcttt ttcgatcaaa tgaattattt tttgtttgaa ccagacaagc
    48121 aaaaagccga tcaaagctcc cccaatacta gaaactacta gactcatccc cgcgtttact
    48181 gctgaaaaag atccggtgat cagcgcacca agagcaaact ggaaggcagt aacaccagat
    48241 gcatcattca aaagaccttc tccttccaaa atatgcatcg cctttttagg gatattcact
    48301 cgtccagaaa gagaactgac tgctaccgca tctgtcggac ctaacgctgc tccaaaagca
    48361 aagcaggcag caagtgggac acttggcaac aggaatgata acgtcgcacc taccccgaca
    48421 agggtcaaaa tgactcctcc aaatgccaag aacaaaattg tcccgaaatt tttgagaatc
    48481 gaagaaatat ctgctttttc tccttctcta aataacaatg gtgcaataat cataactaag
    48541 aacaattcag gttcaaaatc gattgattgt ccccactctg tcagacctaa taccactcca
    48601 aggaaaattt ggatcagtgg agctggaatc attggcaaaa tgcggctagc tatgttcgat
    48661 aaggtgatcg ttactgcaaa tacaataact aactcagcaa attccatcgt ccatcccctt
    48721 tattttttat tttgtgtaag catttttaaa aattgttctt ttttctttaa aattttggtt
    48781 tcgactactt caacctgtgt ttggttaccc tctaattcac actgtacacg ttttgactcc
    48841 aaacgaagca gatcatgttt cagacgttcc aactgacgat acatcaatat atttctttta
    48901 cgcaacttac aaagtatttg acggcggtca tccatcgaaa gggcgccata gcgatcatgc
    48961 aacagttttt tttgttgttc aaaactctct ctcattttcc agatctccta tacacaaact
    49021 tccatcatta tagcaaagtt ctttttggat gaaaaatatt tcattgtttc atcgtttatt
    49081 gctagttaga aatttccata aatttttcaa cgcctcattc tattccaaat agtatcagat
    49141 atcaagtaaa acttaagaaa gataaaagtt tctgacaaaa gaatgtttac ttttccattc
    49201 ctgcctttct tgttctttta tcacaacaaa aaaatggggc tgcgacaaaa atcttgtcac
    49261 agtcccttat tctgaataaa cggtgtggaa gagacagcct atcagtagta ataagacaca
    49321 tctctttcac agcttcattt ttgcgtaaaa atcgatttat tcgaaaaatt ccccgtatac
    49381 atcttctaca cgttgatctg gattgatcac gatatacaag ttacctgttt tttcgctgat
    49441 ttttgttgat tgatattgat tgtctaatcc ttctgttcct ttatctagat atgggaagta
    49501 cacaagatca ggtgtgccat ttcggtaaag gatttccatt cgctcgatat cttcgtattt
    49561 caatgcgcga ttaaacatgc ccatttccag tccgcctagg ttgatatcat gtgtcgcaac
    49621 tgcgtcccct tcatggaaga tttcgatttt aaatccagca cacggatgga tttcaacaaa
    49681 ttcactgcca ttgattcgtc caaaacttgt tgtgatttgt ttgatccaaa gatcgccgat
    49741 atgacggcgg ttgatcgtcc aggtttctcc atttctaaaa taaaatttta gcgctgtcat
    49801 ttctttactc attaaaaaga ctcctttatg atgtaattta ttcttgtact acctcagttt
    49861 agcacaaatt tatgtaagtg gtcacatctt atgttttttc ttctaatgac gtatctgccc
    49921 actttaagta gccaacaaag ctgagcagat acagtctagt gttttttaaa tcaatttttc
    49981 tttcgataaa aaatccaaaa cctgtttttt atcatttttt attctttgtt ctacgacggc
    50041 tttttcagct aattgcaaag cttcactaac ccacttacca ggtttttttc cagtttcttc
    50101 tagcaagatt ttaccatcta ctgctaattc gcttaatgac ttgataggta aacgatcata
    50161 ggattctcct acggcatcca tctgaggtgt ttcgtcaaaa tagatcatca attcttctac
    50221 ttgcagtgca tgttttttgc ctaagaagta caattcgaat ggttccaaag ctccagtttc
    50281 tcgttgtcgt agtccttgga caagtgcttg aacgtcttta atcatttggt tagaacactt
    50341 ccacgatttc aaaaaggaac gaatctcatt ttcttttaga cccagttggc tgatcaataa
    50401 tgtccatact tggctctctt ctttcaatgg gacggatggt aattcagcaa aacgtaaaag
    50461 tgcttcgcct tcttctttca aactgggaca gcaaatgtag cactctgttt caataaatgc
    50521 atggattccc gcggcccgtt catttcccaa gagcatcttg atgaattcca catttatacg
    50581 ttctacagat attttaccta atagctcgtg gttttctttg atggcgtaga aggtttcttc
    50641 ctctaatttg aaccctagtt gactgacaaa acgcaacccg cgcatcattc gtaaggcatc
    50701 ttcatggaac cgctcatgag gattgcctac agctcgcaag atctgttctt ctaaatcttg
    50761 cagaccatcg aacaaatcga tgatttctcc atcttctttc aaagcaaacg cattgatcgt
    50821 aaaatctctt cttttcaaat cttctgccaa cgaacgaacg aattcgacat gatctggtct
    50881 gcggtagtcc tgataagttg attccgtccg aaatgtggtg atctcatatt gttcatcatt
    50941 ttctaatacc aacacagttc catgttcgat cccaatatct atcgtttttg gaaaaagctg
    51001 tttgatttct tctggaaatg cgcttgttgc gatatccaca tcatggattg gctggttcag
    51061 caagatatca cgtacacttc cacctacaaa ataagcttca tatcctgcat tctctaattt
    51121 tttcagaact ggaattgcct tttggtattc cagaggtaat tgttttaatt tcatttttcc
    51181 aacctttcct gcgtgtttct tgtactgaga taaagactca gttgcatggt gactgtttga
    51241 caagtccgtt tcagagtctt caaggccagg accacttcat cttgtttact cgtccttacg
    51301 ctcaaagcga ttatggtctc tttgattgaa tttttccatg tttttttcaa aaacttcagt
    51361 taagtcgata ttcaatgaat tagccatgat catcgtaacg aataaaacat caccaagttc
    51421 ttctgctacc gagttaactg gttcagaagc ctttttgctt ttttctccat aagtgtggtt
    51481 gacttctctt gccaattcac ctacttcttc agttaatcga gccatctgag ctaatggaga
    51541 gaaatacccc accttgaatt gctggatata ttcatccact tcctgctgca tactgtataa
    51601 agaacgcttt ttatccatct ttttccctcc aaaactatct taccaaaact ttaacgcaaa
    51661 tcctagataa gagcagttga gaaaatcgaa tttccatgct acatttagaa aggaatttct
    51721 aaatggaaat tctgtgaaaa tacataattg aaagctggaa ttcatatggt caaacgaaaa
    51781 acagtaaaag aagttctttt tatcattata ggaacaagta tttatgcatt tggtcttgtt
    51841 tacttgaaca tcgctaatca tttagcagaa gggggcgtct cagggatcac cttgatctta
    51901 agagctttat tcggaattga tccagcttat acgactttat taatcaacat cccattgatt
    51961 ttactaggag gaaagatcct cggtaaacgt tccttggcct atacagttct aggtacactt
    52021 tctttgtcat tctttttatg gatctggcaa agggttccac tagaaatcaa tttacagcat
    52081 gatctactga tcgtttctct acttgcgggt ttagttgcag gtgtaggaag cgggatcgtc
    52141 taccgcatgg gtggaacaac aggtggcagc gatattattg ctcggatttt agaacaaaat
    52201 tacggcatca ctatggggcg ttccctattg gcatttgata tcatcgtctt acttgcttca
    52261 ttaacctata tcgacttgaa acgtatgatg tatacgttga ttgtttcgta tgttttcagt
    52321 cgtgtgattg attcgatctt agatggcgga tattctgcaa aaggtctact tgtcgtctcc
    52381 aacaaaagcg aagagattgc accactactg atgaccggtc tgcaaagagg tgtgactttt
    52441 ctttctggag aaggcgcatt ctctggtaat tcaaaaaaaa tcatctatat ggtcgttagc
    52501 tctagtgaat taactgaagc taagaggatc gtccacgaga ttgacgaaaa agctttcctt
    52561 tcaatcatca atgtgcatga agtggaaggg caaggcttta cttatttgaa gccacagaca
    52621 agattactga aaaaactata agaaaaaaga caaagatagg ctatgaggaa a
//
LOCUS       DANKEF010000017        51120 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000017 DANKEF010000000
VERSION     DANKEF010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.017"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(18..293)
                     /locus_tag="PWZ48_001098"
     CDS             complement(18..293)
                     /locus_tag="PWZ48_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260770.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            587..1402
                     /gene="thiD"
                     /locus_tag="PWZ48_001099"
     CDS             587..1402
                     /gene="thiD"
                     /locus_tag="PWZ48_001099"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDL2260771.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKDAQQETVNDYVLLESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(1607..3058)
                     /gene="cls"
                     /locus_tag="PWZ48_001100"
     CDS             complement(1607..3058)
                     /gene="cls"
                     /locus_tag="PWZ48_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2260772.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQKALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            3400..6066
                     /locus_tag="PWZ48_001101"
     CDS             3400..6066
                     /locus_tag="PWZ48_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2260773.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNE
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            6430..7182
                     /locus_tag="PWZ48_001102"
     CDS             6430..7182
                     /locus_tag="PWZ48_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2260774.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(7204..8358)
                     /locus_tag="PWZ48_001103"
     CDS             complement(7204..8358)
                     /locus_tag="PWZ48_001103"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HDL2260775.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            8543..10984
                     /locus_tag="PWZ48_001104"
     CDS             8543..10984
                     /locus_tag="PWZ48_001104"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HDL2260776.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKPYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     KVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(11314..12018)
                     /locus_tag="PWZ48_001105"
     CDS             complement(11314..12018)
                     /locus_tag="PWZ48_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260777.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
     gene            complement(12205..12450)
                     /locus_tag="PWZ48_001106"
     CDS             complement(12205..12450)
                     /locus_tag="PWZ48_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260778.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            complement(12467..13318)
                     /locus_tag="PWZ48_001107"
     CDS             complement(12467..13318)
                     /locus_tag="PWZ48_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDL2260779.1"
                     /translation="MNSLTSTYRLLNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQPTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            13484..14488
                     /locus_tag="PWZ48_001108"
     CDS             13484..14488
                     /locus_tag="PWZ48_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDL2260780.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            complement(14729..15085)
                     /locus_tag="PWZ48_001109"
     CDS             complement(14729..15085)
                     /locus_tag="PWZ48_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2260781.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            15453..16640
                     /gene="tuf"
                     /locus_tag="PWZ48_001110"
     CDS             15453..16640
                     /gene="tuf"
                     /locus_tag="PWZ48_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2260782.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            complement(16828..19419)
                     /gene="mprF"
                     /locus_tag="PWZ48_001111"
     CDS             complement(16828..19419)
                     /gene="mprF"
                     /locus_tag="PWZ48_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2260783.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLVSVLPMIGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDP
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(19445..20206)
                     /locus_tag="PWZ48_001112"
     CDS             complement(19445..20206)
                     /locus_tag="PWZ48_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2260784.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            20373..21005
                     /locus_tag="PWZ48_001113"
     CDS             20373..21005
                     /locus_tag="PWZ48_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HDL2260785.1"
                     /translation="MKRKRLSIIRILFYMIILTVIGAVLFLGGRTYRSVKRVESYQIE
                     ITTAAKNYHMENYENLIKAIILTESKGKGTDLMRSSESLHGEAEIIDNPADSIDQGVK
                     YLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHGGENTVELAEAYSKDVLSPLLG
                     NEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKMNEKIIETYEKVFK"
     gene            21064..21924
                     /locus_tag="PWZ48_001114"
     CDS             21064..21924
                     /locus_tag="PWZ48_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HDL2260786.1"
                     /translation="MEKRQKMENQKVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            22109..24424
                     /locus_tag="PWZ48_001115"
     CDS             22109..24424
                     /locus_tag="PWZ48_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2260787.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            24606..26447
                     /locus_tag="PWZ48_001116"
     CDS             24606..26447
                     /locus_tag="PWZ48_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDL2260788.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            26471..27580
                     /gene="rpoD"
                     /locus_tag="PWZ48_001117"
     CDS             26471..27580
                     /gene="rpoD"
                     /locus_tag="PWZ48_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDL2260789.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            27797..29938
                     /locus_tag="PWZ48_001118"
     CDS             27797..29938
                     /locus_tag="PWZ48_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HDL2260790.1"
                     /translation="MMKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDA
                     AMQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIE
                     KNEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHRED
                     TKEEHAKELLKMISEKIAQENKVEPEHEAEKDTEKDISGVNAAGKESLKTNDLKTNDL
                     KTDIPEKTETLSEKQSEDEKSNGLPEADKKEMLEKADKKATESDGINNEADAETQSAS
                     EDSTALEAKIEKEENQPELIEAETEPITIVPNTEKTPQESNPEKKDLDKTQKKKSKKV
                     PYLALAAVLLLGGGGWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFI
                     RADMIHQDLSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDH
                     LADDIKLKADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVV
                     DSATRDQYKTAKEAVEKVKNTDLSAQLNEQLQQVDKMLTDKEKKEEEEKKKAEEAKKA
                     EKAKKAAEEQAKQAAQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARAS
                     DIADASNPAWTWAPGVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKS
                     ALMKGIGESALPFYIVTINCKTGWFGGNGSN"
     gene            30134..30997
                     /locus_tag="PWZ48_001119"
     CDS             30134..30997
                     /locus_tag="PWZ48_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDL2260791.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            31102..31575
                     /locus_tag="PWZ48_001120"
     CDS             31102..31575
                     /locus_tag="PWZ48_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2260792.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            31671..32558
                     /gene="xerD"
                     /locus_tag="PWZ48_001121"
     CDS             31671..32558
                     /gene="xerD"
                     /locus_tag="PWZ48_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDL2260793.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
     gene            32577..32948
                     /locus_tag="PWZ48_001122"
     CDS             32577..32948
                     /locus_tag="PWZ48_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2260794.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGVYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            32918..33715
                     /locus_tag="PWZ48_001123"
     CDS             32918..33715
                     /locus_tag="PWZ48_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HDL2260795.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            33699..34268
                     /gene="scpB"
                     /locus_tag="PWZ48_001124"
     CDS             33699..34268
                     /gene="scpB"
                     /locus_tag="PWZ48_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HDL2260796.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISFEEDVSPDLFFDGMNKGE"
     gene            34274..34990
                     /locus_tag="PWZ48_001125"
     CDS             34274..34990
                     /locus_tag="PWZ48_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2260797.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     regulatory      35072..35196
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            35319..35930
                     /locus_tag="PWZ48_001126"
     CDS             35319..35930
                     /locus_tag="PWZ48_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2260798.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIP
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     gene            36308..37831
                     /locus_tag="PWZ48_001127"
     CDS             36308..37831
                     /locus_tag="PWZ48_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HDL2260799.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            38068..39408
                     /locus_tag="PWZ48_001128"
     CDS             38068..39408
                     /locus_tag="PWZ48_001128"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HDL2260800.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            39562..40299
                     /locus_tag="PWZ48_001129"
     CDS             39562..40299
                     /locus_tag="PWZ48_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2260801.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGNSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(40464..40685)
                     /locus_tag="PWZ48_001130"
     CDS             complement(40464..40685)
                     /locus_tag="PWZ48_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HDL2260802.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            40716..41747
                     /locus_tag="PWZ48_001131"
     CDS             40716..41747
                     /locus_tag="PWZ48_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260803.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYERILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            41734..43137
                     /locus_tag="PWZ48_001132"
     CDS             41734..43137
                     /locus_tag="PWZ48_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDL2260804.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGFLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVIHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKHQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            43203..43838
                     /locus_tag="PWZ48_001133"
     CDS             43203..43838
                     /locus_tag="PWZ48_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2260805.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            43887..44567
                     /locus_tag="PWZ48_001134"
     CDS             43887..44567
                     /locus_tag="PWZ48_001134"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDL2260806.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAIQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            44702..45931
                     /gene="rpsA"
                     /locus_tag="PWZ48_001135"
     CDS             44702..45931
                     /gene="rpsA"
                     /locus_tag="PWZ48_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDL2260807.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            46113..47423
                     /gene="der"
                     /locus_tag="PWZ48_001136"
     CDS             46113..47423
                     /gene="der"
                     /locus_tag="PWZ48_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDL2260808.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            47666..47941
                     /locus_tag="PWZ48_001137"
     CDS             47666..47941
                     /locus_tag="PWZ48_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HDL2260809.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            48176..49222
                     /locus_tag="PWZ48_001138"
     CDS             48176..49222
                     /locus_tag="PWZ48_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260810.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQRPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            49224..50483
                     /locus_tag="PWZ48_001139"
     CDS             49224..50483
                     /locus_tag="PWZ48_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDL2260811.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIAPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            50489..51070
                     /locus_tag="PWZ48_001140"
     CDS             50489..51070
                     /locus_tag="PWZ48_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HDL2260812.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
BASE COUNT        17065 a         8836 c        10759 g        14460 t
ORIGIN      
        1 taaagaacag ttagaagtta aaattgctga ttcacataga gtttatgact tcttggaaat
       61 tctttttcaa tcagtttttt catctgttta tgaaattctt ttttttcagt aaacataaag
      121 gtttgggtat cgtaaccttc ttcatgtagg aaatagatta ttcctttttc tgtgttttgc
      181 gtataagctt ttatactcct gtcttttttg aaagaacaga tcgtatagcc atctatttgg
      241 tctggtagtc gtttgatttt cttcaaaatg acttttttat cctcaagttt catttttttc
      301 acctgacttt atttttttca tatggaaatc atagcgcctt tggttatatg aaaacaagaa
      361 ggatactgaa atatatataa atcaaatagt ggttatttat tattagaata gcgaatacaa
      421 acaaatgaca actattttct gatttattac aaatgttttt tcaaataaat ttgattacca
      481 agaaaaggta gtggtatatt tactgaggga tactttgaaa aacatatgat agaaacagta
      541 acagcattat gaaaaatctt tttataaaaa tggaggaatc gatttgatgg aagatcagta
      601 tgtacaagca atgacgatcg caggatcaga tagcgatgga agcgcaggga tgcaagcaga
      661 tatgcataca ttctttacaa gaaatgttta cggtgtttct gtcatgaccg catgtgttgc
      721 aggtaattct tacggaatcg gagcaagtgt gacattgcca acagatttta tcgacaaaga
      781 atttgaactg attgcgaagg actttcaaat acgtgcagct aaaacaggta tgctagcaga
      841 ttcaaagttg attgagacag ttgtaaaaaa ttaccaaaaa tatgattttg gaccattagt
      901 cgtggatccc gtaatcgtaa cgaaacatgg aaatctttta ctagaagaat ctgctttgca
      961 aagcttgaaa gaaaagcttg tccctttggc cgaagtactg acacctaatt tttatgaagc
     1021 agaaaagctg actggacgga cattagcagc tgaagaagat tttgtgcaag cagcaaaaga
     1081 actgcaagat atgggtgcaa aaaacgttat gatcaaagga catcatgcta aagatgcgca
     1141 gcaagaaaca gtcaatgact acgtattgct cgaatcagga aaatcatttt ggttaaatgc
     1201 accattttat cctaccgacc ggatcaacgg aactggcgat tcgctatctg catgtattgc
     1261 agctgagtta gcgaaaggaa aaacagttga acaagcgatc cgattggcaa aagactatgt
     1321 aaaccgtgca attcgtgaag aaataaatgt agggcacaaa tatggaccca tcaatcattg
     1381 ggcagctggt gaattagagt aaaagaaaga ggtcgtgagc ctgccgtcca gctccgagtc
     1441 acaaggaaca agttcaaaaa acagcttctc atgtttgttg aatattgtga cttgtgaccg
     1501 aggagttggc aggagaacac cgtttgttcg gaagaaggga ctgtgacaag atttttgtca
     1561 cagtcccttt aatcttagta tatttttctc tacttgaata aaaaaattat aggattggag
     1621 aaaataatcg agaaatttgt tgcttgaata atagccagtt agacatttcc ttgactgttt
     1681 cagttgtcat ggatgaacaa tgtgtctcat cttctttgaa ctgagtagca agttcttcta
     1741 aaaactcagg atcgtaaatg acagcacttg tttcaaaatt caatcggtag ctacgaatat
     1801 cttgatttgc tgaacctacc atgcagattt catcatccat tatcaatgtt tttgcatgga
     1861 ggaagccgtt ctcatagaca aggatttgta cattttcctt gatcagctgc cgagcataat
     1921 attgtgttgc tcgataaata aaaggatgat ccggtttgtt gggaatcatg attttcacat
     1981 ctacaccaga ggcagttgcg atttttaaag cagcaatgac actttcatca ggaacaaggt
     2041 aaggtgtctg tatccaaaca cgttttttag cagaagtaat caatttgata aatgaaagct
     2101 tgatttgttc cctttcgtta ttcggtccgc ttgaaacaag ctggatggat gtatcagcaa
     2161 gcttagattc atctcgatct gcttttttaa agaaataatt caaatgatag ccgacctttt
     2221 tttcttcagg taccgagacg ttccagtctg ttaagaagcg aagctggagc aaagaagaag
     2281 cggcaccgaa aatccgtata tgcgtatcgc gccaatagcc gaattttttt gttgtattta
     2341 catattgatt ggcaatattg aaaccaccgg tataactaat ctttccatcg atcacaacga
     2401 tttttcggtg atcatggtaa ttcaatcgga aacgaagtaa tgccttttgt gaggtaacaa
     2461 atgtatggac atgtccacca tttttgatta gacgattgaa atcttttgct tttgtaccat
     2521 gagagccaaa tgcatcatat aatattcgaa cttccacgcc ttctgcggct ttttcttcta
     2581 aaacatgtaa gacttgctgg ccgatattat ctgtcacaaa tgcgtaatat tcgatatgga
     2641 tcgaatgttt ggcttttttt agatcttgaa gcagtgaatc caatttctct tgtccgtctg
     2701 tgagaagagt gacagaattc attcttgtca gcggcatacg atttaatgaa gagaagaagt
     2761 cgacaaattg ctgtttgtct ttatcgccca tgtcaggatc gtaatgttca atggtatctc
     2821 ctctaaaaga ctgaaagttt tctaattctt tcaagtcact ttgtcggaga tagaattttt
     2881 tcttatccgt taatccacgt ccgaaaaata aatataatac aaagcccacc ccaggaaggg
     2941 caaacaatac taagagccat gcccaaatgg ctgctacatc tcggggtttg atcaaaatag
     3001 ccaccaaagc aataagtgca tttaatagat aaagggcggt tataatacta gataccacaa
     3061 aaatgttcct ccttacatac aataacatta gtatagcatg atagaagaaa gaagcctacc
     3121 aaggtgaata tacttttcac tcctttcatg aaaatgtagg taaatcgggc taaagtagtt
     3181 gctttttgta ggttaataag gtaggatatt agtacagttg aatatctcct taggggagta
     3241 accggcaacg tttgttgcga taatatcaac agtaagttaa ccaaatggtt ttctggtatt
     3301 gtatgaaatg gtgagaccta agacaaatgc gtatttgtct taggtctctt tttttttcag
     3361 acaggactag ggccaagatc aaaaaatggg ggatgaaatt tggaaaacaa ggaacaagca
     3421 caacaagcgg tctataaaga aacgatcgac tcgttgatgc agcaaacgca gagctcaacc
     3481 gatggattga gcaccacgca agcgaaggaa aaattagaaa gagatggctt gaaccagcta
     3541 aaagaaaagc cgaagaagcc aacttggaaa ttgttcttag aaactttcaa agatgcaatg
     3601 gttatcgtat tgctgattgt cgcactgatt cagatggtca tgggtgacta catcgaattc
     3661 ttagttattt ttgcagtatt gatgttgaat tctataatca gtgttgtcca aacaaaaaaa
     3721 gcagaaggtt ctttagaagc gttgaaaaac ctttcagcgc ccgatgctcg agtgattcgt
     3781 gatggtaagg aaaaaacgat tccggcaaac gaacttgtgg taggagatat cgtgttgtta
     3841 gacgcaggcg attatgtacc tgctgatgga cgtttactag atgcgggatc attaaaagta
     3901 gatgaaggta tgctgacagg agagtcgact tctgcagaaa aagaagtaac ggatatcaca
     3961 agtacggtac caatcggaga tcgtacaaat atggtattca gtggaacgat cgtaacttac
     4021 ggacgcggta aattcttagt gacttcaact gggaataata cggagatcgg acaagttgct
     4081 ggccttttgg aatcaacaac tgagcaagtc actccgttac aacgaggtct ggataaattc
     4141 agtaaaaaac taagtctggc tattttggca ttgtctttat tgattttagg tatccaactg
     4201 tttcgtattt atcttggaga aggtacagga gatatgactg cagatatcgt cagcgcagtg
     4261 atgtttgccg tagccgtagc cgtagctgct attccagaag cattacagtc tatcgtaaca
     4321 atcgtgttat ctttgggaac gaataaaatg gcaaaacgtc atgccattat tcggaagctt
     4381 ccagcagttg aaacacttgg ttcgacaagt atcatctgta cagataagac tggtacattg
     4441 acacaaaaca aaatgacagt tgttgactac tttttaggta atggcgatac tggggatttc
     4501 acaaatgacc cttcaaaatg gacagctgac gaacgtcgct tgatcgaaat cagtgtttta
     4561 gcgaatgatg catcgatcag tgaagacggt actaaattgg gcgaccctac ggaagtagcg
     4621 tttatcgact ttagtgaaaa attaaaccag ccttatcagg aaatccgaaa caactaccca
     4681 agacaagcag aacttccttt cgattctgat cgaaaattaa tgtctactgg acacacgatc
     4741 gataacgaaa attacttgtt tgtcaaaggc ggaccagata tcgtcttcca acgcagtaaa
     4801 aaagtattgc taaatggtga agtcgttccg atgacggatg aattgatgaa aaaattccaa
     4861 gagcaaaatg aagctttttc aacacgcgca ttacgtgtac tagcttttgc ttaccgtcca
     4921 cttgataatt ttgatttatc atttgaagat gaagacgatt tgatccttgt tggtttgctt
     4981 gctatgatcg atcctccacg tgctgaagtg actcaggcgg taacagaagc aagaagtgca
     5041 ggaatcaaaa caatcatgat tactggtgac cacaaaacaa cagctgttgc tatcgcaaaa
     5101 caaatcggga tttttgaaga aggaaacctg gctttgaccg ggacggaact agatgaattg
     5161 acagaagatc aactaaacga agttttagaa aaagtcactg tttatgcacg tgtttcacct
     5221 gagaataaaa ttcgtatcgt tcgtgcttgg caaaataaag gtcatatctc agctatgaca
     5281 ggtgatggtg taaatgacgc accagcattg aaacaagcag acatcggaat tgctatggga
     5341 acgggtacag atgtggcaaa agatgcagca gcgatggtat tgacggatga taactttgct
     5401 tctattgtca gcgcagtaga agtaggacgt aatgtgtttg ataatatcaa gaaagctatt
     5461 tcttatctat ttgcaggaaa tttaggtgcg atcattgcga tcatctttgc cttagttgct
     5521 gactggtcca atccatttac agcattacaa ctgttgttca tcaatttagt caatgattca
     5581 ctgccagcaa ttgcattagg tacagaaaaa gctgaacctg ctacgatgaa ccgcccgcca
     5641 agagatccaa attcaggaat ctttacggac aaaacattga tttctgtaac gtatcgtggt
     5701 gtgctgattg ggattgctgt tatctttgct caatggcttg gcatgcagca ttcacctgaa
     5761 ttcggtgtag ccatgtcatt ttcaacattg atttggtgtc ggacattgca aacattccca
     5821 gcacgttcaa acacacaaac agctatccaa gcagggttct tctctaacag aagtgtctta
     5881 ctagctgtag ttgtatgtag cgcgctttac tgcttaacaa tgatcccagg actgaaagaa
     5941 gtcttctcta tcccatcagc atttggattg acagaagtct ttacatgtat gggaatcgca
     6001 cttgtgacaa ttgttttgat ggaaatcact aaattgatca ttgtacgagt acaaggaaat
     6061 aaataaaacg aatcaacgag gttgtgagcc tgccgtttaa ttctgagtca caaggtgcaa
     6121 tttcaacaaa cagcttctca tgtttgttga atattgtgac ttgtgaccga agagttggca
     6181 ggagaacacc gtttaaggaa gaagttgtaa aatcagcatt ttgagctaat actgagggtc
     6241 atctgattgg acactgttta ttcggaaaga gggactttga cacgactttg gtcacagtcc
     6301 ctttttttat ttatgataga cggaaagcca atcattttat tattcatcaa aagagataga
     6361 atagataaaa gaggagaata atttattccg aaaagtgaaa acatataaca aaaaattaga
     6421 ggaaatcaaa tgaatgaaaa agaatgggat gaatttgctg aagaatatta tgaaatccag
     6481 caagaatcgc aagtaactat tgctgaagat gtaaaaaatt atctgaaaac ggagaaagtc
     6541 ctgcctgctg aaacagcagc agacgttgct ggaggatctg gcagatttct ttccttagca
     6601 aaagaaatta atcgctatga tttgatcgat ttttcttcag gaatgctaga aaatgcaaaa
     6661 aaagaagcag aaaaaaatga gttgccaaat gtttccttta tcaaacagtc gttagaagaa
     6721 tttatccaat caaatgagac atatgaaatg gtttttacag cagcaaatcc agcattggta
     6781 catcttattc aattagaaaa attgatgaaa aagtcaagca aatggtgcgt gataatcagg
     6841 gtagtggaat caatcgatga tgttttttat cctatcgaaa agcaactagg aatgatagaa
     6901 gaagatccta atacatcgcc agttgtaatg gatcaatttg aaacatactt aattgatgaa
     6961 ggattcaaag tgaagaaaaa agattttact tattttacac aggaatcaat tactgaagaa
     7021 ttgatgaagc tatactatga cgcgtacgca aaagatcctg ttgtgaccag gtacattcgc
     7081 aaactattca gtgaaaaacg agaaatcctt agtacgacta agctgaccta tagactgctc
     7141 ctttggtccg cagataaaaa aagaaccttt cacgactcgt gaagggttct ttttatgatt
     7201 ttattatatt ttgtagtaac gaatatcatt ttcgatcttt gtaatcgaaa aaggtacatc
     7261 tgaagtatat tctgcacatt ctccgtgttc aggaagcgtc tcactgaaaa tcgtttcata
     7321 ttcgtcaaca gtgatccgtg ttcgagaatc tagcatctct tgatgagatt gtgcgaagag
     7381 atattcttga tagttttctt ccagataacc tgtaaaaaat tcacttactg caccagaacc
     7441 gtagctgaat agaccgatgc gatctcctgg ctgtagtgat ttcgaatttt ccagtagaga
     7501 agttagcccc aggtataatg aaccagtgta aagattaccg attcgtcggc tgtaacggat
     7561 gctttcttca tagcgagcca taagacgttc ctgattttct tcgtctgttt ggtctaatac
     7621 gctttgcaat gccttttttc ccatcttagt atacggaata tggaaagcaa tcgcctgata
     7681 atcttcgagt cctcgacccg acaattcttt atgtcgattc caaacttttt ggaatgattc
     7741 gatatacgta gaattagata aaggaccatc aacaacagga aattcgctat aatctggacg
     7801 ccagaaatca tagatatctt ctgtcagaaa tacgctgtcg tcttcaatcg ataaaatacg
     7861 cgggttttga gtgatcatca tcgcaacagc accgacacct tgcgtcactt caccaccgct
     7921 tgccaagccg taacgagcaa tatcacttgc tatgactagt acttttcgtt ctggatgatt
     7981 tttgacatat tctttcgcca tgtgcaaagc tgcggtgcca ccgtagcaag cctcttttac
     8041 ttcaaaagaa cgagcaaaag attggatttt cagtaaatgg tggataatta ccgcactcgc
     8101 ttttgaatga tcaatcccgg attctgttcc gacgatgacc atgtcgatta gctcacggtc
     8161 tttttctgtt acgattttgc tggcagcatt tgctcctagt gtaatgatgt cttcatttgc
     8221 acgattgact gccatttggt cttgaccaat ccctatatgg tattttgcag gatcatcccc
     8281 acggctttct gccaactctg tcatatctaa atataaatta ggaataaaaa aggaaagacg
     8341 atcaatccct attttcattt caacacctct ttacttatga taaaaaactt actttactaa
     8401 ggtatcataa aaagattaaa agaaatataa aaaagagaat atttaaggat tgttaaaatt
     8461 taatcttaga aaaaaacaac tggaacaaga gctatttgtt tttacagcaa agaatggtat
     8521 atttttgtag gaggtggctt acttgaaaga agtcgttatg atagatgctg caagaacgcc
     8581 gataggaaag tatcgcggaa gtttaagtcc atttactgca gttgaattag gcacacttgt
     8641 gactaaagga ttattagata aaacaaaact gaaaaaagat aaaatcgatc aagtaatatt
     8701 cggaaatgtc ctgcaagctg gtaatggtca aaatgttgcc agacagattg ctttgaacag
     8761 cggtctccct gtagatgtcc cagcaatgac gatcaatgaa gtttgcggat ctggtatgaa
     8821 agcagtgatc ttagcacgcc agctgatcca attaggtgaa gcagaacttg tgatagccgg
     8881 cggaacagag agtatgtctc aagcacctat gctgaaaccg tatcagtcag aaacaaatga
     8941 atatggtgaa ccaatttcca gtatggtcaa cgacggattg actgacgcat tttcaaatgc
     9001 acatatggga ttaaccgcag agaaggttgc aacacaattt tctgtgagca gagaagaaca
     9061 ggatcgctat gccttgtcgt cccagttgaa agcagcacat gctgtcgaag ccggtgtatt
     9121 ttctgaggag atcatcccag tcaagatttc tgatgaagac gtgttatctg aggatgaagc
     9181 agttcgtgga aatagtacat tggaaaaact gggcacgtta cgtacagtat tctcagaaga
     9241 aggaactgta acagcaggaa atgcttcgcc attgaatgac ggtgcctctg tggtgatcct
     9301 tgcatccaaa gaatacgcag aaaataataa tctgccttat ttagcaacca tcaaagaagt
     9361 agcggaggtc ggtattgacc caagtatcat gggaatcgct ccaatcaaag ccattcaaaa
     9421 attgacagat agatcaggaa tgaatttatc aacaatcgat ctttttgaga tcaatgaagc
     9481 atttgcagct tcttctatcg tagttagcca agaactgcag ttagatgaag aaaaggtcaa
     9541 tatatatgga ggagcaatcg cattaggcca tccaattggt gcaagcggtg cacgtatctt
     9601 gactacatta gcttacggat tgcttcgtga acagaagaga tacggtattg cttcattatg
     9661 tattggtgga ggtttaggct tggcagtctt gttagaagcg aatatggagc aaacacataa
     9721 ggatgtgcaa aaaaaaaagt tttatcagct tacgccatct gaacggcgca gtcaattaat
     9781 agaaaaaaat gtattgacac aagaaactgc gttgattttt caggaacaaa cactttctga
     9841 agagctctcc gatcacatga tcgaaaacca agtgagtgaa gtggaaatcc caatgggcat
     9901 cgcgcagaat tttcaaatca atggtaagaa aaaatggatt ccgatggcaa cagaggaacc
     9961 atctgtcatt gcagctgcaa gtaatggtgc aaaaatctgt ggaaatatct gcgcagaaac
    10021 acctcagcgg ctgatgcgag ggcaaatcgt tctatcagga aaatcggagt atcaagcagt
    10081 gatcaatgca gttaatcatc gaaaagaaga gctgattctt tgtgcaaatg aaagttatcc
    10141 ttcaatcgtg aaacgcgggg gcggtgttca agatatctct actcgggaat tcatggggag
    10201 tttccatgca tacttatcta ttgatttttt agtggatgta aaagatgcaa tgggcgctaa
    10261 catgatcaac tcgattcttg agagtgtggc aaacaagtta cgggagtggt tccctgaaga
    10321 agagattcta ttcagtattt taagtaattt tgctacagaa tcattggctt ctgcctgttg
    10381 tgaaatccca ttcgaacgct tagggagaaa caaagaaatc ggtgaacaaa ttgccaaaaa
    10441 aatccaacag gcaggggaat atgcgaagct tgacccttac cgtgcagcaa cgcacaacaa
    10501 agggatcatg aatggtatag aagctgtagt ggcagctaca ggaaatgata cacgtgcggt
    10561 tagcgcttcg attcacgcct acgcggcaag aaatggatta taccaaggat taacagactg
    10621 gcagatcaaa ggggataaac tagttggaaa actaacggtg cctttggctg ttgccacagt
    10681 aggcggagct tccaatatcc tgccaaaagc aaaagcatct ttagctatgt tagatattga
    10741 ttcagccaag gaactagcac aagtaattgc tgcagtaggg ttagctcaaa acctggctgc
    10801 tttgcgtgca ttagttacag aaggaatcca aaaaggccac atgggacttc aagctcgttc
    10861 attagcgatc tcaattgggg ctattggtga ggaaatcgag caagttgcga aaaaattacg
    10921 tgaagcagaa aagatgaacc agcagacagc tatacaaata ttggaaaaaa tccgggaaaa
    10981 ataaaaaagc aatatcttta gaagaataaa gcaagattta cagttatttt atttactgca
    11041 aatcttgctt ttttctctat cagaaaatat gtttattatt agaatttttt atagttaagg
    11101 ttaggcatct taagaaatca ggcaactcca agatatcttc aggtagaaga gaagtcgcct
    11161 aacattgtag gatcataggt gatttgttgg acaggtcaag ctgatatttg agcctgatgt
    11221 tagaaggaat ttctttttac ttgctgtaga aattattata tgactaaaaa ggctgggatt
    11281 ttaaatccca gccttttatg acaaatagaa tttttatcga tcagtaatcg ctgtcgctgc
    11341 aaattgttcg atcaaaggaa tcaaagtcaa tttgactttg tatttccgtt cgagtgcttt
    11401 ttcgataacg aactcatttt cagtagtcat agaaatagtt gctaatttac gaggttcatc
    11461 agcatttttt tcattaataa ctacttcatg ttcattttgt tctgtgtaaa atttaggata
    11521 ctcagcaacg gcttttgaaa aatcagcaaa acttaaagcg gcattcgtat cagttgagcg
    11581 tgtattttca gttgtgtccg cttttggttt ttccgttttt tcttctggca cactgactgt
    11641 ttcttctgta actggtgtcg caacagcttg cgcagttggt tcatcgtttt tcaatcgttg
    11701 atcattccag taagacacga tttcaataag aagattgtca cggaatcgtt cgaaaatccc
    11761 gttttctcct gaacgaattc tctttaatac agtattgaag ttgtcatcga ttggcacgtt
    11821 gatccgtgta tcttcatttg ttttcccagc taatccgatc gttaagattt gacggttttt
    11881 gggatcaagc atgacttgta acggcaaacc atagcgtcgc aagctctctt cttctagaga
    11941 acggaataat tgtcctctta caccatccaa tgaaggacgg aattgattta ataatttttg
    12001 tttctgtttt ttcttcatat gttctctcct tcgctcatac cctttcgatt ataagaaaaa
    12061 aacagccaaa tgaaaaggag taatgcaaaa aaacttgcgt atacgcaaat tacaacagaa
    12121 aaatttgcgt attttcccgt tttttgaaag aaaaataaaa aatggaacgg attatgtata
    12181 tatacataat ccgttccatt ctttctattt tttcggtatc aaggcaaaaa caggtagttc
    12241 aggtgaataa atgacttcat gttcgtgcca gggtttgacc attagcgaat gatcgaaccg
    12301 ttttttcagt tcttcgatga tttgatcatg tgtccagttt cttgcaggaa aatgttggat
    12361 tttctctgca tcgatcaaaa agacaaaatc ggaatgaaaa gtctgggtaa cttcttccga
    12421 gagagaattc aagcgttcga ttggatccat aaaatcacct ttcgacttaa aattgggtag
    12481 tatctgggtc ttttgctttg ccgactacac catctaactg atcgatttgt ttcatttctt
    12541 cttctgttaa ttcaaaatca aataattcag tattttcttt gattctgctt ggtgtcactg
    12601 acttaggaag aggaaggaat tcgtgttgca gcgaccaacg caaagcaact tgtgcaatcg
    12661 tcttgtcatg tgcttcagcg atttgtttca tttcaggcac atcgaagatt ttaccagtgc
    12721 ctaatggact atatgcttcg aggatcatat catgtttttt tgcatactca actacagttg
    12781 gctgtaattc gcctggtgca aggaaaactt gattgaccat cggcataatt tttgctgttt
    12841 ttgctagtgt atctagatgg tggggtaaga aattgctcac accgattgct ttgattttac
    12901 ctgcttcata cagttcttca aatgctcgcc atgtatctgc atttgcttgt tcccaattat
    12961 cacgaaaagc aacagggttt ggccagtgga tcaaaaacag atctaaataa tctgtttgga
    13021 gcttcttcaa tgattcttca aaagaagaca tcacaagttc atagctgtgg tttgcattcc
    13081 ataattttgt tgtcaagaat atctcttctc taggaattcc actttcctta atcgcttggc
    13141 cgacactttc ttcattcttg tatccttggg cagtatcgat atggcgataa cctgcagcca
    13201 gcgcttcttt aacagaggaa acagcgacat ccccatcagg tgtttgccat gtgccgaatc
    13261 ctactaccgg gatttcgtaa ccatttaata gacgataagt gcttgttaat gaattcatac
    13321 tttcagctcc ttttttattc gttcaactat aagtgtagcg catttttcga aaaaaagaat
    13381 ccattttgtt tctactcttg aaatttttgt ttaaaagaaa atgattttga ttctggtatt
    13441 tttgattttt tttccgtaaa ctaagacaga agggaatgag aaaatggata tctatttaaa
    13501 aaaagcaatt ctgcatatta ttgacagaga aactggttct ccagtctttt cacaaaaaga
    13561 gctggatctg acaaaagaat atattcgtga ttttcttcaa aagaagatcc aaaaaatctc
    13621 ttctgctcaa acaaaaactg gacaactagc agaagaaagc atgatcgttg aatcacttaa
    13681 aatcgctcaa gaaaattttg tggaagcaag tgaagcaatc gttcagcggt ggttcgatat
    13741 ctaccaagaa agtgaagaag cacctagtgc agatgtattt gttgttttgt atgaattaga
    13801 tacagtgatg tacttatctt tattgaaagt caattatcgt gaggcttaca ctcactttgt
    13861 tgaagcagac gaagcaggga tcgacaaccg cttgattttg aatcgagcaa ttctaggggc
    13921 gaaaacacaa aaagcagatg aagcattcgc tgtgaatttg actgattgga cctacgaatt
    13981 aattgaaaaa aaatatgagt tttctggcaa aaaagagctt tatttttctg gaagagtgat
    14041 cgaaagtgtt cctgcacctt ctttagaaga aaatgtaaaa gtgatccaaa aagtagccaa
    14101 gcatcttgga aaaaaatttg agacagaaga atttgatatc atggctgatt tgaaagaagc
    14161 tgtctatgat acgatagaag aaaaaggtcg gttggatcat gagatgattg cggaaaaagt
    14221 cttcaaggaa aacatcacgg caaaaatggc ttttaaagaa gaagtacaag agcaaggatt
    14281 tgtcccagaa gcacctcctg taaaagaagt acaagaaatt tctgagaaaa aatttggaaa
    14341 acaaaaattc aagctatcaa acgggatcga actgatcgta ccagtagatg tatatcgcaa
    14401 cccagatttg attgaatttg tgaataatcc ggatggtacg atatccgtga tgatcaaaaa
    14461 tgtggatgaa gtattgaatc gtttataaaa atgactagaa cattaggtgt tcaagtacca
    14521 gtgatcagtt aaatgaattg tgtcaagaga aggatcggaa aagacgaata taacaaaaaa
    14581 agagcgtgag acaaaagtaa agaacacttt tgtctcacgc tctaaacacg tataaacggc
    14641 ggaagcagaa gcaactcctt cttatttctt ggagttaaac gcttctgtcc cagcctcttt
    14701 ttatctaata aaattttaag accatttact aattatcgat tttttctgcc gcatttttag
    14761 gcacttcaga agcctttatt tcttcccagc ttaatacacc tttacgagga ttgactttga
    14821 gtttaagata cgcattcatc tttaatggat gttctctttc ttcgcgaagc gtcacctgtt
    14881 ttttttcacc atctttatta taagccgctt gcttgtaggt ataagtcgtg aatttctctc
    14941 cgttatctgc ttgggtagct gacatttcgc cattcgtcgt gatttttgta taataagctt
    15001 cgccgccgta aaaataatga taagctgcaa aacttccgcc gagaaatatg cctaaaacga
    15061 caagtacgcc aattaatttt ttcatgtact tcacctcttt ccttattatt aacgaaatag
    15121 aagatttcgt ctattgaaag ttgttggcaa cagctgaatt ttaaactttt cctcattttg
    15181 ttcttattgc ttcaaaagaa agttaaggac tatcttgaag tttttctcaa aaaacacatt
    15241 tttttattta taaaaggtac ttttttcgcg tataagacaa gcgagtgctc ttagttgact
    15301 ttgaccaata ttgacgtata ataagcttag ttcgaatctt acaaaaagta agtcggacgt
    15361 attctttttg atttagtcaa aaaaagacta taataaaaag gtgagagctg aaatttttca
    15421 gtgatctata ttatttagga ggtttgtaaa ttatggcaaa agaacattat gacagaagta
    15481 aaccacacgt taacattggt acgattggac acgttgacca tgggaaaact actttaactg
    15541 ctgcgatcac tacagtatta ggcaaaaaag gcctagcaaa tcctcaagac tatgctagta
    15601 ttgatgcagc tccagaagaa cgtgaacgtg ggattacgat caataccgca cacgtagaat
    15661 atgaaacaga aaaacgccac tacgcgcata ttgatgcacc aggacacgcg gactatgtga
    15721 aaaacatgat tacaggggca gcacaaatgg atggtgcaat cttagttgtt tctgcaactg
    15781 acggtccgat gcctcaaaca cgtgaacaca ttttattgtc acgccaagtt ggtgtaaaat
    15841 acctgattgt tttcttgaac aaagttgatt tagtcgatga tgaagaattg atcgatttgg
    15901 tagaaatgga agttcgcgag ttattgagcg aatatggttt cccaggcgat gacactcctg
    15961 tgatcaaagg ttccgcatta aaagcattgc aaggcgatcc agatgctgaa gctgctatta
    16021 tggaattgat ggatacagta gatgaatata tcccaacacc agaacgtgat acagataaac
    16081 cattactatt gccagtggaa gacgtcttct caattacagg tcgaggaact gttgcctcag
    16141 gtcgtattga tcgtggtgct gttcgtgtcg gtgatgaggt agagatcgta gggatcaaac
    16201 ctgaaacaca aaaagcagtt gtaacaggtg tagaaatgtt ccgtaaaacg ttagattacg
    16261 gggaagctgg ggataacgta ggcgtgttgt tacgggggat ccaacgtgac gatatcgaac
    16321 gtggacaagt gcttgctaaa ccaggttcca ttactccaca tacaaaattc aaagcagaag
    16381 tgtacgtgtt gacaaaagaa gaaggtggac gtcatactcc attcttcaac aactatcgtc
    16441 cacaattcta cttccgcaca actgatgtta caggaacaat cacattgcca gaagatacag
    16501 aaatggtcat gcctggtgac aacgtgacga ttgatgttga cttgatccat ccaatcgctg
    16561 ttgaaaacgg aacgacattc tcaattcgtg aaggcggacg gacagtgggt tcaggtatcg
    16621 ttacagagat cgaagcataa gaaacgcgac tcgaatattt tggtaatgaa aaatgtttaa
    16681 aataaaagag cggtccaaac aggatttctg tttggaccgc tctttgttct tcaattgtat
    16741 aagaagagat tgtagaagca tgaactttcg ttttacttca gtctctttta ttcatcatga
    16801 gagtgttcat ctatcggtat gcagttttta atactttctt cgtatccatt ttttcaaacc
    16861 acttattcta ccttgtgtct gctcaactgg cgcattatca ataatcagca aagcaatcat
    16921 aacatatgca atccaactat ctctagaata tagagtataa cgtgagaccc agtttgtagc
    16981 gtacttgctt ttatattctc ttaatccatg gaaagaataa aaatgagaac caaactcgta
    17041 aaccaagtat gcaatccttt cctgtaaaaa actctttctg gaagttccga catttgaaag
    17101 aggggccatc cctaaattga accattggat atgttcttct ttcatatagg caaacaaatg
    17161 caagaacaag aaatccatac tgccagaagg tgctttttta ggatcatatc gcatcagatc
    17221 aatcgttcct actttattgt tcgtgtaagt aggcatgatc gttgcaaaag cgacaatttc
    17281 ttcttgggta tttttgacaa ctgcaatagg cgctctttgt agataatctt cagagaaaaa
    17341 gcctaaagaa aacccttttt cctttcgtga acccaaccaa ttatcagaga tatttttgaa
    17401 agtactcatt tgctcagtag aaaatggtgg ttgtaagaca tcaaatgtaa atccttctcg
    17461 ttcgatccga ttcaagacag cacgagtgcc cttcattttt ttccctgaag ttgtaaaact
    17521 attcaggtct acataagctt cttcccccat cttaatgaaa tcgtagccga attcatgcaa
    17581 aatcataaca atctcttcgc ttgtttcgta gaaaactggt aaatagcata gtcgatctgt
    17641 ttcttcgata aaagcttcga ttgcttctgg aaaatcttct ttcttaccag aaggatcacc
    17701 cattacaata catttattat tgaagcaagc gaattggaga agaacagttg gttcaccgtc
    17761 tttttcatag gcaaacatcc gcttatcttt taagaagatc aattgactat cggagtttcc
    17821 tccataagtt gtaaggatct tcagggcctt ttcttcatta aaggcttcgc caatctgttt
    17881 cttttctcct tgaaggaaat gaacaaatag aacgatcatg aaactaacag cgatgatggc
    17941 caacaatccc gaaaaccaaa tcttttcaga aggaaataag aaaaaggaaa taaaatggtg
    18001 acgccgatga gggaaatccg gcagattgta taccccaata acgatgtaaa gcaaggacag
    18061 tgcaccaata atgattccat cgatcgtacg ccactcccat gaatagatca attgttcccg
    18121 aaataattcg tttttagaag caagaatgat cagaagcaga atagacaaga aaaccaccgg
    18181 tgtgaaacta aaatcactta ataaaacata aagtaaagct agaacaatca agaagattgt
    18241 aggcaaatat gctcgtttca cacgtgctgc gatccctctg cccataatga ttaaaagaaa
    18301 tcccaatagt atactaggaa attggataat aaaatgaaat ttcaatgggt tcaaactatg
    18361 aagccagcga aactctgtaa aagcttgcgg gatagtggca gaaagtacta gcatgattcc
    18421 agaaaagtat aataagacaa ccacgatttt atgcgcaatt tctgttgcta attgtttagg
    18481 gataccagaa taacgctgat caaaagtgga accgatattt ttgaagaaaa agacgattcc
    18541 aatcaaaaac ggaatgatgt agtagaacag gcgatagagc aagatccaca caacgactat
    18601 ttctcttggc acaccaatag cggacaaccc gataatcatc attacatcga aactcccaat
    18661 ttctcctgga atcatagaca cgattcctat cactgaagca gcaatgaata acggaattgt
    18721 ttgccacaga tcaatccgga tttccattaa ataaccaaca gaaaggaaac taaacaggac
    18781 accggtccat tccaacaatg aaactagcaa tagctgtcct tgagttttaa gtgaggtacc
    18841 accaaggaat cctttctttt tgaaagtagt aaataaaaag acgacaggaa aatatagacc
    18901 gccgccgatc aaccatatcc aatattgctt caaaaaagga gcaactggcg tgaagactac
    18961 aagtaaaaaa gaaagcagac tgtaaataga aagtccagcc atcaaaaaca agaaaacttt
    19021 cgataatgct tggatcacgt ttctgctctc tttcttttgt ccatatagtt cagagcgaag
    19081 ccccacacta acaaacccgc cgaaaccggc aatattgttg attgtattga tcagccagct
    19141 tgtctcaaac aagtaacgag gattctgctt ttgttcaagc atccgattca aaataatatc
    19201 ataaccaatc ataggtaaca cagagacaag accaatcaac agcataagtc cgcttttcca
    19261 tacaggtata gtggcaaacg tttctcctaa ttgctggata gatagagttt taccgatgga
    19321 catcaattca ccgacaataa tgacaatgac agaaatcaaa aagattgttt tgaacagtcc
    19381 taggtggttt ttcagccatt gtatggtatt ttttaacaaa taaatcacct tctattccta
    19441 atagttatgt ttcagaaaga aattgaagaa cttctcgatt gaagcgttca ggatcttttc
    19501 tagctaattc atgtccttgt tctttcacaa gcacaaagga agcttttggt atagttttag
    19561 ctatatacaa agaatgtttt agcttgatta catctttttt tcccacgatg atcaaagtgg
    19621 gggagttgat tttttttaaa tcattttctg taagcccgat gtcatgcagc aaaagtttga
    19681 taactaaaag atttttgcga agacttgggc gaaataaaga gagtaaccat acccatgcat
    19741 aatgaatatt actgatgact ctggcagaaa aacgaacgcc tttaacaagc gtgtttccag
    19801 aatttaaaat caatcgatga acttttttag ggaagctgct ggcaaatacc agtgctagat
    19861 ttgctccatc actaaatcca agaaaatctg cctgatcgat tttttccagc aacatgatcg
    19921 tattcagatc ctcggccatt aactgaaaat tcaacataga tgcttcgttt gtcgaacgac
    19981 catgtcctcg gctatctact agatacacgg tatagtagcg ttcgagtaca gggacctgtt
    20041 ctgaaaaaaa gcggccgctg ccgtcattgc catgcagtaa aaagagaggg aagccttgac
    20101 cgctcttttc atagtaaatt tcgctcccat ctggcatttt tgcatatttc ttttcttttt
    20161 tcacataatc acctactgtg taaagtataa cgattatatc agtcattggg caattgtttt
    20221 tactgaaaaa caacgatcat tttcgtaaga tgacaaaaaa tacaggaaaa ttcatttttt
    20281 cttaaggcgt acacagaaat caaggaagaa atagccaaga agataaagaa atagtaagat
    20341 aaaaaaagat gtcaaaataa ctaggtgatg aaatgaagcg aaaacgatta tctattatcc
    20401 gtattctttt ttacatgatc attctgacgg ttattggtgc ggtgttgttt ttaggaggaa
    20461 gaacttatcg ttcggtaaaa cgagtggaaa gctatcaaat agaaataaca acagctgcaa
    20521 aaaattatca tatggaaaac tacgagaatt tgatcaaggc gataatattg accgaatcaa
    20581 aaggaaaagg gacagatctc atgcggagct cggaaagtct gcatggagaa gctgagatca
    20641 ttgataatcc agctgacagt attgatcaag gtgtcaaata tttggcggaa atgatagtag
    20701 aagcaaagaa acaaggatgc gatttatgga cagctgtcca agcatataat tttgggaaag
    20761 attatattgc ctatgtaaaa aatcatggcg gagaaaacac agtagaatta gcagaagcgt
    20821 attctaaaga tgtgctgagc ccacttttag gaaatgagga agggaaacaa taccgatatt
    20881 ggcggatcca gtccttgctt tacaatggcg gctatctcta tcataatgga ggtaacatgt
    20941 tttatcctga tattgtcaaa atgaatgaaa aaataataga gacctatgaa aaagttttta
    21001 agtaaaatag caggaaatgt tcaatcatga ataaaattaa atgaaagaag gagtgcgcaa
    21061 atagtggaaa aaaggcaaaa aatggaaaat caaaaagtgg ggtacacaac caaagactgg
    21121 atgttacggt ttgtgaaggg aatgtttatc ggttcaggat ttatcttgcc aggagtcagc
    21181 ggtggtgcat tagcagctgt ctttggcatg tatgagcggc tgatttcttt tttagcccac
    21241 cttacaaaaa atttcaaaga aaatgttttg ttttttctgc ctgttggtct aggcggagtg
    21301 acaggcatct ttctactttc ttttttagtc agttttttat taggtgcgta tgaaacaacg
    21361 atattatggt tttttgtcgg atgtatcgct ggaactgttc ctgcgttatg gaaagaagca
    21421 gggaaaaaag gaagagatac ttctgatgtg atcatcatgg ttgttacttt tgttctggct
    21481 tatttgtttt tattatacgg cgctcaatta tttgacgggc aagtcgatca gaattttgga
    21541 acatggctga ttgccggcgg attgattggt ttgggtatga tcgtcccagg attgagccct
    21601 tctaattttt tggtgtatat gggattgtat aaagcgatgg ctgacggatt taaagaagta
    21661 cgacttgaag tgattgtgcc gatcggaatt ggtgggattc tttgtgttct tggattgtca
    21721 aaaatcatgg attacatttt ctcaaaggca tatagcaagc tattccattt cattttaggt
    21781 gtagtttttg cttcaacgat tatgatcatt cctaccgatt acgcgggatt gggagttgct
    21841 ggggtactag cttgtcctgt tctttttata gccggtacag cactaggttg gtggatgagc
    21901 cgtttggagg aacaatataa gtaaagatag attgataata aaaacagcac acgggtgatt
    21961 caaacaaaaa ggttggcatc ttttttgtaa aatcattcgt gtgtttttct ataaatttta
    22021 taaaaacaag ttgtttttaa tcttggaaca cgctagactt aaacacgtga ttatttttga
    22081 aagttggcgg aaaatggaga aaaagaaaat gaatggactt acacagcaag aggtagttca
    22141 gaaaatggct tctggccagc aaaatgacta ccaggaagat gcatcaaaaa gtacaaaaca
    22201 aatattcagc gataatatat tgacgttatt caattttctg aactttggta ttgggatttg
    22261 tctgttattt gtaggtgcgt attccaacat ggcgtattta gcaattattg tcgtcaatat
    22321 cgttattgga atttatcaag aaatacatgc aagaaatctt gtacggaaat tatcgattgt
    22381 ttcccaatca gaagtactag tgatccggga cggtaaagaa caatcgattt ctactaaaga
    22441 gctggtatta ggagatttgg taaaaatcgg agcaggagaa cagataccag ccgatttaca
    22501 ggttatatct ggacgggcag aagcaaatga agctcttttg acaggcgaat ctgatttgat
    22561 tgtcaaagaa actggagctg aattgctttc tggaagcttt ttgacaagcg gttctgtcat
    22621 ggctgaagtt atccgagtag gtgcagataa ttatgctgtc cgcttgacta gcgaggcaaa
    22681 gactcacaaa gctattcgtt ctgaacttgt agattcgatt cgcaaagtat ccaaattcac
    22741 tagtttcgtg attattcctt tggggatcat actatttttg gaagcactgt tcatccgcga
    22801 tgcttctatc aaaatttctg ttattgcatc tgcagcagct ttgttaggca tgcttccaaa
    22861 gggtttggta ttgctaataa gtattgcatt aacgacaggg gtaaccaaac tggcaaaaaa
    22921 acgaatcctt gttcaagaca tgtattcaat tgaaacgctg gcccatgtcg atacattatg
    22981 cttggataaa acagggacca ttactgaagg aaaaatgagt atccagcggg tagaagcact
    23041 acatccgctg tacgagaaag ccatcccaga aattatgggg acgtatctgg cagaaagcag
    23101 tgataacaac atcactatgc gggctatgag agattatttt gagctttcta atcggtatga
    23161 tgtgacaaac aaagtcgcat tttcttctga tcgcaaatgg ggcgcgatgg aatttccaga
    23221 actgggagtc gtgtacgtag gagcgcctga acgtttggtg gaagaagatc ggcttccgga
    23281 cagtctaaaa acagcccaag cgaatggcta tcgcgtactt atgttaggga tcgctccaaa
    23341 tcagcaattg aacgaagaaa aaccagcatt tgttgagccg gttgctctgt ttgaaatcga
    23401 tgatccaatc cgaaaaaacg ctcaagaaac actagcttat cttcatgagg aaggtgtgga
    23461 tctgaaagtg atttctggag acaatccttt tactgtctcc aatatcgcta gacgtgcagg
    23521 attaccgaat tatgatgcgt atgtcgatct ttctcaaatt actgaagaaa tggaagtgcg
    23581 agaagctgct catcgctata cggtttttgg acgagtgagt cctcagcaaa agaaattgct
    23641 ggtaaatgaa ttgaaagaaa gcggcagaac ggttgcaatg acaggcgacg gtgtgaatga
    23701 tgttttggcg ttaagagaag cagattgtag cattgctatg gcagaaggtg atggagcaac
    23761 acgtcagatt gcgaatctgg tattgttgga ctctgatttt actacattgc cagaagtcct
    23821 gtttgaagga cgccgagtgg tcaacaatgt gacgaaagtc tcaggcatct tttttatcaa
    23881 aacgatttat tcatttattt tatcgattat atgtgccgtt acagcaattg gatttccgtt
    23941 tatccctatc caaatcacgc tgattgattt agcgattgaa gggtatcctt ccttcttttt
    24001 atcttttgaa caagataaaa gaaaaatcac ttaccgttat ttgcctacag cacttattaa
    24061 tgctttacca aatgccttgc ttgtagtagt gaatatcatt gcggtgtatc tacttggaaa
    24121 aacattcgca tggggaaatg ctgaaactac tactttgatg tattacttgc tgattgggat
    24181 cagttgcatg gcagttgtaa aagcttgtta tcccttcaac ccattgcgca tcttcctagc
    24241 agtaacgacg atcgtaggga tatatgtagc tgcaatgctg ttccatggaa ttttggaagt
    24301 aaacttaggg ttgggagaga gcgtaggata cttctttgta ttcatgctcg taaatattgt
    24361 tcttcgagta ttgtactgga aggctgatat tcaaaatcag ttactgaaaa gagtagcaaa
    24421 ttgatgctta atgagataga aatgtgttat acttgaaaaa gcaatgaaat atgatacaat
    24481 caaacatgta taattgtgtc ctttaaaatc gcactgattt cagttagtgc gattttttac
    24541 cggttattct cctgaaatat cttctgttaa acccaaaaga tatcaataga gcagggggtg
    24601 gaaaaatggc tcaacggatt cctcaagagg ttatcgaaac agtacgcagt caaacgaata
    24661 tcgttgaggt gattggacaa tatgtccagt tgaaaaagtc tggtaaaaat tacttggggc
    24721 tgtgtccttt tcatgaagaa cgtacaccgt ctttttctgt tgcagaagac aagcagattt
    24781 ttcattgctt tggttgtggt aagggcggaa acgtgtttac ttttcttcaa gaactagaag
    24841 gtctaagttt tccagaagca gtggtaaaag tggcagactt cgaacagata ccgttagaag
    24901 atcaatggcg tcagcgtact atagctgtcc aaaatcctga atctgctacg gggaaactga
    24961 ttgctgctca tgaaaaagca gcggaaatct atcatcacat gctattgcat acaaaagctg
    25021 gacagccagc tttagaatat ttgcaaagtc gtgggttgtc tttagaatta attgaagaat
    25081 tcaacatagg ttttgcacct aatgagcgtt cttttcttca gcaagtattc aagaatgaat
    25141 caatttcaaa tgaattgtta gaacgctctg gattgtttgt cacgcatgat gatggccgta
    25201 tgtctgatcg tttttatcaa agagtgatgt ttccacttcg taattctcaa ggccagacga
    25261 tcggtttttc aggacgctgg cttgcacctg aaaaagggca ggaaaaagat cagcccaaat
    25321 acttgaatag tccagaaaca gaactgttca ataaaagaca agttttattt aatcttgata
    25381 aagcaagaag cgatatccgg aaaaacggag aaattttgtt gttcgaaggg tttatggatg
    25441 tgattgctgc atggcaggct ggtatcaaaa ctggattagc ttctatgggg acaagtctaa
    25501 cagctcaaca gatccaacaa atggaaaagc tgacgaaaga attggtcttt tgctatgacg
    25561 gagacaacgc aggttttgaa gcaaccaatc gagggattga attgcttcgg caaaacagtc
    25621 gcttagcact ttctgtcgta gtcgttccag aaaagcttga tcctgatgaa tatctccgta
    25681 agtatggaga agcatctttc caagaattag tggctcatgg gcgcgaaaca gtctttactt
    25741 ttaaatcacg ttatttaaaa caagggaaaa atttagataa tgaaaaagac aagatcgaat
    25801 acctcgaaga attactagtt gaacttagcc gagttgtttc accaatcgaa caagatatgt
    25861 atctatctca actgtcaaca gagtttcaga tcggcagaga gacgatacaa aaacaactgc
    25921 gcgatcttcg aaggacaaat cagcaaaatc gtcaagaacc tcaggaaagt caacacactc
    25981 aggttacgag acaagaaact gcacggaaaa agagaccttt gacgcaagta gaaaaggcag
    26041 agcagattct attatatcgt gcatttaaag aagtttctgt ccgtaatatt ttaaaagaac
    26101 gagaatttca gtttattcat gatgcatatg cagaagttta ttttttattc gatgcatatg
    26161 tctcacaaca ttatgaattc aaccttgcag agtttttaga ctttttgcaa gatgaaaatt
    26221 taaggcggtt ggtggtggaa atcgaatatt taccggtggc agaagaaagt tcgccacgag
    26281 aaatccaaga cttgctagat gtgatcagca aatctggctt agctgatgaa ataacattaa
    26341 aaaaacaaat gcagcaagaa gccagacgaa ctggaaacaa acagcttgag cttgaattga
    26401 cgattgaaat catcaattta acgaaacagt taaaacaagc caaatagcta ctcaaggggg
    26461 gccttctttt atggcaaatg gaacagataa aaaaacgtat gaagcagcag tagcagcttt
    26521 catcaaagaa aacaaaccca aacgacaagt tgtatatgat gatttatcca atcagcttgc
    26581 tacaccattc actttgaatg cagatgaaat ggacaaattg atccaaaaga ttgaagacgc
    26641 aggtatcagt gtggtggatg aaaatggtga tccatcaatt cacagcttga agagtgctga
    26701 gaaaaaagct gaacaagcaa aaacagaaga tctatctgcg ccaaccggtg taaaaatcaa
    26761 tgacccggtt cgtatgtatc tgaaagaaat tggccgtgtc tcccttttaa ctgcggaaga
    26821 agaagtagcg ttagcattga aaatcgaaga aggcgaccaa gaagcgaaac agcgtctggc
    26881 agaagcaaac cttcgtttag tagtaagtat cgctaaacgt tatgtaggac gaggcatgca
    26941 gttccttgac ttgatccaag aaggaaacat gggactgatg aaggcagttg aaaaatttga
    27001 ctaccgcaaa ggatttaagt tttcaaccta tgctacatgg tggattcgtc aagccatcac
    27061 tcgagcaatc gctgaccaag cacgtacgat ccgtattcca gttcatatgg ttgaaacaat
    27121 taataaattg atacgtatcc aacgtcaatt gctgcaagac cttggaagag aacctacgcc
    27181 agaagaaatc ggtgcggaaa tggatcttcc aacagaaaaa gtccgagaaa tcttgaaaat
    27241 cgcgcaagaa cctgtttcac ttgaaacgcc aatcggtgaa gaagatgact ctcacttagg
    27301 tgatttcata gaagatcagg aagcaacaag tccagcagaa cacgcagctt atgagttact
    27361 aaaagaacaa ttggaagatg tactagatac attgactgac cgggaggaaa atgtccttcg
    27421 cttacgtttt ggtttagatg atggccggac gagaacattg gaagaagtag ggaaagtatt
    27481 tggtgttact cgggaacgta ttcgtcagat cgaggctaaa gcattaagaa aattgcgcca
    27541 tccttctcgt tcaaaacaat taaaagattt cttagaataa gcatgaacta ttgaccagaa
    27601 gagaaatcag taaaactgtt tggtttattt taaaagaaaa aaagcttgag actacgcagt
    27661 ctcaagcttt tttttctata ggaaggaaat tcattcatta tttttaagta aatcctaaaa
    27721 ataagctatt ggcgtgacgt atagaatatg atagattaaa tggtagcgta aacctagtaa
    27781 aggagccgat cgctaagtga tgaaaaaatg tccaaattgc ggatcgaaaa aaatggagca
    27841 gaatactatc tgtccagact gcggtcatca aataactgca gaagatcagc aaataaaaga
    27901 atcaattgaa gaaacaaata cagatgcagc tatgcaggat aaatcaactg aagatgcagt
    27961 ctttcctgat acagaattaa acgatccaat cgaatggtcg gaattgaaag atttacctct
    28021 agaatctgta atggaattat tcgaaaaagc agaaccagaa gacgaagaac cacctgctca
    28081 cgatcaagta actaatatag agaaaaatga acagaagaaa gcaactgttc atgagacttc
    28141 ggaagaatca ggaaagaatc aagaagaaac gcttgaatca aaacaaaaaa gaagagtagc
    28201 tgaactgaaa gaaatcgtcg ataatgagga agaaaattcg atactatccg cttatatcaa
    28261 agcacatcgt gaagatacaa aagaagagca cgcgaaagag ctgctcaaaa tgatcagtga
    28321 aaaaattgca caggaaaaca aagtagagcc agaacatgaa gctgaaaaag acactgaaaa
    28381 agacattagt ggagtaaacg ctgcaggtaa agaatcatta aaaacgaatg atctaaaaac
    28441 gaatgatcta aaaacggata tcccggaaaa aactgaaaca ttgtctgaaa aacaatcaga
    28501 agacgagaaa tcgaatgggt tgccggaagc agataaaaaa gaaatgctcg aaaaagcaga
    28561 caagaaggca acagagtcgg atggaataaa taatgaagcc gatgctgaaa ctcaatctgc
    28621 aagtgaagat agcactgctc ttgaagctaa aatagaaaaa gaagagaacc agcctgagct
    28681 gattgaagca gaaacagaac caataacaat cgtgccaaat actgaaaaaa cgcctcaaga
    28741 atcaaaccca gaaaaaaaag atttagataa gacacaaaag aaaaaatcaa aaaaagtgcc
    28801 gtatctcgct ttagcagcag ttcttctact aggtggggga ggctgggctt attatgacca
    28861 tcatcaaaaa gtagaagccg aagtagcagc agaagcaaaa agacagcaac aaatggaaga
    28921 acttcatgag aaacttgctg ccttttatac agatgatcac catcaattta ttcgtgcaga
    28981 tatgattcat caagatttat caaaactaaa aaatgattta tccagtctta agaatgaaaa
    29041 aggatattca gagttagaaa caacttacca agatatccaa tctaagatcc gagatatcca
    29101 aaaagtgaat gagtggttca tagcacctgc catcgtcgat gatcatctag cagatgacat
    29161 caagttgaaa gcagataaag agatccaagc aatcgaaacg gataacacag attttggaaa
    29221 attgattcaa caagcacaaa cagaagctaa aaatcaatat gctgaattgc aaacagctaa
    29281 agaaaaaaca gctatagtct ttaaagacgg aaaagtagtg gattcagcaa cacgcgacca
    29341 atacaagacg gcaaaagaag cagtagaaaa agtgaaaaat acagatctat ctgctcagtt
    29401 gaatgaacaa ttgcaacaag tagataagat gctcactgat aaagagaaaa aagaagaaga
    29461 ggaaaagaaa aaagcagaag aagcgaaaaa agcagaaaaa gcgaaaaaag cagctgaaga
    29521 acaagcaaaa caagccgctc aagcagcagc tgcagctcaa gccgctcaac aagctgcggt
    29581 cgcacaagca gcacagcaag aagcggcatc ctcgacccct tcctcttcaa actcagcgaa
    29641 tcagccaatc aaatctgcca gagcaagtga tattgccgat gcatctaatc cagcatggac
    29701 gtgggcacca ggtgtccaaa gcaaagtgat tgctacatgt atccaacgtg gatatatcgt
    29761 tgaaggagga tacagattag aaaaagccag aatcgaaaat ggggaaggct attacaatct
    29821 atatgccact tcaacaaaat ctgcattgat gaaaggcatt ggagaaagtg ctttgccatt
    29881 ttacatcgta accatcaact gtaaaactgg atggttcgga ggaaatggga gcaattaatt
    29941 ttaggcaaat gaaagaggct gtgagcctgc cgtttaacac cgtttattcg aaaaagggga
    30001 gtatgacaca actgttgtca tactcctttt ttcacctttc caaaccaagg ctcagctatt
    30061 caagtagata acattatgtt atgatagcaa agaaatgtat taagaaaacc tttcactata
    30121 gagggagaaa aaaatgaacg atttattagc aactatattt acaggtatta ttattgatga
    30181 aaatgaaaaa aactactttg tccaaaaaaa cggacaaacc tttggcttaa gcaaatcaga
    30241 aggtgaacac aaaatcggtg aagcagtgga aggattcggc taccaaaatc aaaaacatga
    30301 aaatcggatc actacacaaa ttcctaaaag ccgtatcggt cattacgctt ttggaatcgt
    30361 tactgctaca cgaaaagatt taggtgcgtt tgtcgatatc ggtcttccag ataaagactt
    30421 tgtcgtatct cttgatgaat tgccgacaat gacagagcta tggcctaaaa aaggggatcg
    30481 cttgatgatt tcccttagag ttgatgcaaa agaccgtatt tggggcagtt tagcagatga
    30541 aaaaatattc aaatctttaa gcaaatatgg aacagaagaa atgaaaaaca aagatgtgat
    30601 aggaacagca taccgcttga aattaacagg tacgtacgtt ttaacggaag atttctatct
    30661 tgggtttatc catccttctg aacgatataa tgaacctcgt ctaggcgagc aaataaaagg
    30721 tcgagtgatt ggtgtccgac cagacggtgt actgaatctt tcattgaagc caagagcata
    30781 tgaagctata tcagatgatg cacaaatgat tttaaccatt ttagaacgtt ctgcagatca
    30841 gcaaatcaac tttacagata aatcggatcc agaagaaatc atgaaagctt ttggtatcag
    30901 caaaggagca tttaagcgcg cattaggtaa tcttttgaaa caaggattga ttacacaaga
    30961 aaacggtgtg acaaaactag tcaaaaaatc taattgagaa tcccttgcat gtttttctta
    31021 actagcttat aataatttta attaacatag tgttatatct ttttttattt gtaattatta
    31081 taaattgttg gggttgagta aatggtcatg aatgctattt cggcgatgaa aaaaacaaaa
    31141 cagcagctac atgaagcggg gtttaagctg acaccgcaaa gagaagctac tctgcttgtg
    31201 cttttggaaa atgagaaaga gcatttatct gcagaagaaa tcttcttttt agtgaaaaaa
    31261 aagagtccgg aaattggact tgctactgtt tatcgaaccc ttgaaatctt gacagaattg
    31321 aaaattgtcg ataaagtaag ttttaatgat ggattagccc gttatgattt gagaaaagaa
    31381 ggcgccaaac atttccatca tcatttacta tgcttgaact gcggcacgat cgaggaagta
    31441 gaagaggatc ttttacttga agtggagcaa gtagtagaag aacgttatca ttttttagta
    31501 aaagatcatc ggctaacgtt ccatggaatc tgtcaggaat gctatgaaaa agaaaaaaga
    31561 aaccagtccg tctgagtgtc tagacggctt tttctttgct cagattatgg aaaaagacta
    31621 tcagaatgaa gcggtatctg atatgataga gaggcatgag ggggattatt atgaaagagc
    31681 aaatcaacga ttatctccac tacttgaata ttgaacgggg attgtccgcc aatacacgac
    31741 aaagctatga acgtgatttg gaacagtact tgcattttct acaggagcag aaaattgctt
    31801 catggcagga tgtcgatcgc tttttagtca tccgttttct ggagaagttg catgaagaaa
    31861 aaaaagcgac agcaactgtt actcggatga tctcaagtct cagaagattc catcagtttt
    31921 tacggcaaga gcgtatcact gatcatgatc cgatgcaaca tatcgatacg ccgaagaaag
    31981 cacagaaatt accaagtaca ttgactctga cagaagtaga aaagctgatt gaagcacctg
    32041 atacgacaaa aacgttaggg ataagagatc gtgcaatctt ggaagtcatg tatgctacag
    32101 ggatgcgcgt cagtgaattg attggattga aattgggaga tcttcatctt tctcttggac
    32161 tggtacagac agttggaaaa ggagataaag agcggatcat tcctttaggt gattatgcga
    32221 tccaatggct ggaacgctac ttagacgaag cacgtccatt acttgtaaag gacgcatctg
    32281 aaatgcatgt atttgtcaat aatcatggga aaggtctttc tcggcaagga atctggaaaa
    32341 atctgaagca gctagtacga gaggctggga tttacaaaga agtaacacca catacgctgc
    32401 ggcatagctt tgccacacat ctgctagaaa atggagctga tttgcggaca gtacaggaat
    32461 tgttaggtca cgcggatatc tctacgactc agatttatac acatatcacg aagaaaagaa
    32521 tgacggatgt ttataagcag cattttccaa gggcttagtc ataggaaggt gagaagatgt
    32581 taacacatta ccggaaagaa aaccgtaaaa ttgctatggg actattgagt ttccataaga
    32641 aactgacagg taaaacttct ttgttgaagg agattgatac gtacgaatct gaagacgatt
    32701 atgaactttt tttctttacg ccagaaggtt caaccaacat tcaagggatt attggtgttt
    32761 actgggcaac gaacgatcag ctgatcatcc atgacatttc tttgaatccg tcttatcgcg
    32821 gtgagaatat gggctttcaa atgttagatg aattacagga aaagtacccg gatgtctcca
    32881 tccgtgcaac tgaaatcaca gagccttaca ttggaaaatg gctagcaaag aaaaagaaga
    32941 aagagtgaat gagttgacgg agatcaatat taaattagat gtattcgaag gtcctctcga
    33001 tctgctgctt catctgattc agcaaatgga aatcgatata tatgatatac cgatcgctac
    33061 gatcacggag caatatatga attatatcca tacaatgaaa acactagagc tagctgttgc
    33121 aggagaatac ttagtgatgg ctgctacatt gatggcaatc aaaagcaaga cattgcttcc
    33181 tgtccaagaa ttcgtcacag atgatgaaga attatgggaa gaagatcctc gagaagaatt
    33241 ggtcactcag cttttagaat accgtaaata taaatatgca gctgaaagac tgacagaaaa
    33301 agcagaggaa cgaagtcttt attacacaaa agaacctatg aacatcgatg ggttagcgga
    33361 tgctgaaaag ccgttgaagc gaggacaagt caaaaaaatc gatttgatcc gtgccatgca
    33421 gaaaatctta gaacgtaaga aattttctca aaaagtggaa aaaacgatca cgcccgatga
    33481 cacaacgatc gaagaacgaa tggtatttat cgaagagcgt ataaaaagaa atgcgcatgg
    33541 ctgtactttc gattcttttt ttgagtcacc ttctaaaagt gaagttgtca ctacttttat
    33601 ggctttgcta gaattgatga aaaaagggca gatcattgcg caacaaaaag aaaactatga
    33661 atcaattatg ctttatccag cagtaggaga taccgaacat gacgaaatta agtgaacttg
    33721 aagcccttct ttttattgtt ggcgaggaag ggatcagtaa tgaagaatta agcggactgt
    33781 taaatctcag cgaagcagaa gtcacaagtt tagtgaaaga attaaaagaa tcttacactc
    33841 aaaactcgca ctcagctttg catatttttg aaacggaaag ccattatgta ttaacgacta
    33901 aaaaagagct ggctccctta cttaagcgat atgcgcaagg gacatcaaac acattatctc
    33961 aagccgctgt cgaaacctta gcaatcatcg cttataaaca gccaatcaca agagctgaaa
    34021 tcgagcagat tcgaggagtt caggtttcag gagcggtaca gcgtctagct gcccataaac
    34081 tgattgaaga aaaaggtaga gtagaagggc caggtcggcc gatattatat ggaacaactg
    34141 gctacttctt agattatttt ggtttgaaat cattagaaga actgcctgat acacaagaaa
    34201 tggaaatttc atttgaagaa gacgtgtcac cagatctatt ctttgatgga atgaataaag
    34261 gagaataatt gtaatggaaa gattgcagaa agtaattgcc cacgctggca tcacttctcg
    34321 tcgaaaagca gaagaattca tccttgcagg acgtgtaaaa gtaaacggac aagttgtaag
    34381 ggaattaggg ataaaagttg ggaaacatga cactgtggaa gtggatgaag tcccaattta
    34441 tcaagaagaa tacggttatt atttattgta caaacctaaa ggagttattt ctgctgttgc
    34501 agatgacaaa ggaagaaaag ttgttacgga tcttttgccg atggtaaaag aacgtatcta
    34561 tcctgtaggt cgactggatt acgatacttc ggggatcttg ctactgacaa atgatggaga
    34621 ttttgctcag cgcctgacac atccaaaaca tgaagtggat aaagtttacg ttgcaaaggt
    34681 aaaagggatc gctacaaaat cgatgcttgc acctttgaca aaaggaataa agatcgaagg
    34741 acgaaaaaca tctccagcta aattccaaat tttatcagtg gatcctaaga caaaccacag
    34801 tatcgtggaa ttgacgatcc acgaaggacg taatcatcaa gtgaaaaata tgcttcaagc
    34861 agttggtctt cctgtgcaaa aactaaaacg tgaaaaatac ggagatctga ctttacaagg
    34921 cctacgtcct ggagattacc gtgaactgaa caaaaaagaa gtcagcagtt tgctgaacca
    34981 ctcaaaatga acacttactt tttataagtg ttagatgttg cgttttttga atagatatgt
    35041 ataatgaata tgtaaacaaa aattatataa ggttcgtctt caggggcagg gtgaaattcc
    35101 cgaccggtgg ttatagtcca cgacctattt tgcaaaaaat agctgaatcg gtgaaattcc
    35161 gataccgaca gtatagtctg gataaagaag atagagctta tttgacgacg cttgatcaaa
    35221 taactctaac tctattttcc ctggaaaata gagttttttc atttggagag aaacgtcaaa
    35281 aagttcgctg aagaggagtc ctaacaggag gatttcaaat gaagaacacc cgtgtacaga
    35341 aaatggttgc agtagcgatg tttgctgcga tcgcgttagt tttacagtat tttgcttttc
    35401 cgatcatgcc ggcttttagt tttttgaaga tcgattttag tgatatcccg gtattgatca
    35461 gcatgttctt attcggtcca gcggccggag ttgcaacagc gttcttgcgt tcagtattac
    35521 atctgctgac tacaggtttt tctccggata atttggtagg agacactgct agtttcttat
    35581 caacaacgat ctttacattc ccgatttatt atttctttag tgtaaagaaa cagcataaag
    35641 gaagaaacaa atttttaggt gtcattacag gaaccatcgc tctaactatt tttatgagta
    35701 tcgcaaatta tttcgttatt acgcctcttt acttaatgtt tttaggactg aacgcaacac
    35761 agatgctagg catgtcttta gcaaactatg tactgattgg gatcgttcct ttcaacttga
    35821 tcaaaggatt catcgtgagc gctgtctttt tagtgctgca tgcgcaactt cttccatggt
    35881 taagtcgaaa acaacatcag ttcgaacaac gtcatacagc agtcaaataa ttatcaaaaa
    35941 gatcaagaaa tttcttggtc tttttttttg gaaaaaaata caagacatgc aataatgtaa
    36001 acgttttatt tgtttgtttt gagtattttt ttgatttatg cataaaaatc aatcaattct
    36061 ttgcaaatgt gaaattcttc tccttttaaa agacgaaaat gtttgtttat aaaaaaaagg
    36121 aaacgctaca atgaaaaaga agaaagcttt aaaaacctga aaaaagagga gggaataggg
    36181 aaaaacgaac tttttgaaca aaagttttac aaaatgcgtg ttattatttt tgttctgata
    36241 ctctttttga taaaataaca ttagagccaa aattgttgtc tctaacggaa aagaaaggaa
    36301 gaagaagatg gaacaaacaa aaaagaaaaa atttcaaatg ccttcagcgt atacggtctt
    36361 atttattatt attgcagtaa tcgctgtact tacttggttt ataccagctg ggcactatag
    36421 tgtagacgat gcaggcaaca tcgtcgctgg atcatatgaa cgagtacaac aaaatcctca
    36481 agggctgtgg gatattttca tggcgccaat caatgggatg ttaggtgtac cagcggggga
    36541 gctaactaaa gaaacacctg ctgcaatccc aatctctttc ttcattttgg ttgttggtgg
    36601 atttttaggt attatcaaca aaacaggggc actagatgcc gggattgctt cagttgtgaa
    36661 gaaattcaaa ggaaaagaaa aaatgcttat tccagtattg atgctgttat ttgcattagg
    36721 cggctctact tacggaatgg cagaagaaac gattccattc tatgctttac tgatcccagt
    36781 tatgatggct gtaggctttg atacagtagt agcagtagca atcgtattag tagggtctca
    36841 agtaggctgt ctagcttcaa cggtaaatcc ttttgcaaca ggcgtagctt cacaagctgt
    36901 tgggatttcg atgggtgaag gtattggtct tcgattcttg atgttcgtta ttttgttagc
    36961 tatttctatt gtatatgtgt atcgttatgc atcaaagatc gaaaaagatc caacaaaatc
    37021 attagtatac aaccaacgta aagaagatat gaagcatttc gatattgaag caatcggtcg
    37081 tcaagaagta atcacgaaaa aacaaaaaca tgttaatgta ttgttcttta ttacttttgg
    37141 tatcatgatc atcagtttga ttccttggac aaccttgaat gcgaatttca cgatttttga
    37201 atcattgaca aactggttaa caaaccttcc ggtactaggt actgttctag gtaaaagcat
    37261 gcttccatta ggtgactggt acttcccaga aattactatg ttattcttag taatgtccat
    37321 cgtagtagca atcacttatg gcatgaaaga gtctgaattt gtctctgaat ttttagctgg
    37381 tgcttctgat ttgatcggtg tagcaatcgt agtagctgtt gctcgtggta tccaagtggt
    37441 tatgaacaat ggattaatca cagacaccat cttacattgg ggcgaacaag gattaagttc
    37501 attatcttca agtgtattca tcattattac atttattttc tatatcccta tgtcattctt
    37561 gattccttca acttccggct tagctgctgc aacaatggga atcatgggac ctatgggtga
    37621 atttgccggc gtcggtaagg atttagtaat cactgcttat caatctgcat ctggtttagt
    37681 aaacttaatt acaccaacat ctgctatcgt aatgggtgct gtagcaattg ctcgtttcga
    37741 tgtttcagtg tggtggaaat ttaccggtaa attaattgcg atattatttg tagccatctg
    37801 tgtgatccta ggtgtagcag caatgttcta aacctagaca aatacaggta caaggtctat
    37861 cataagaatg aagaaaccga taaacggcta tcgataagca cgtggacaag tcaatagctc
    37921 cattcagtga gtgttttttc tttcatcatt tgttaagaac attctgcttg ctgatggagc
    37981 aggcttaaaa cgtcattatt tgagaagaag aggttgtgtc ggaggaattt gacatgaccc
    38041 tttttaacat attaggagga taatatcatg aaaaaatttg tgacagaaca acatcatgaa
    38101 caagcattag aatcattgag cgaattgatc cgaattcctt ccgtactaga tgaagcagat
    38161 tcaggacaag gacatccctt cggtaaaaaa gtgattgaag ctttggataa agtattggaa
    38221 atcagtgaaa agctaggatt tagaacattt aaagatccag aaggctatta cggttattca
    38281 gagattggct ctggagatga attattcggt atcttgtgtc atatggacgt tgttccagca
    38341 ggggacgaaa ataattggga aacaaaacca ttcgatccaa ctataaaaga cggctggtta
    38401 gttggtcgcg gatcccaaga tgacaaaggt ccttctattg cggcaatgta tgccgtcaaa
    38461 gctttgattg atgctggcgt ggaattcaag acacgtattc gttttatctt tggtacagat
    38521 gaagaaaatt tatggcgttg tttagaaaaa tataacgaaa aagaagaagg aattacgcaa
    38581 ggatttgcgc cagatgctga atttccttta atttatgcag aaaaaggctt attgcaagct
    38641 tacttgacag gaccaggaac gaacgagttt tctgtaaaag ctggaggagc gttgaatgtt
    38701 gttcctgatg cagctcctta ctctggagaa aagctttctg aagtaaaaga agcgttgaaa
    38761 aaacacgaat tcgatttcga agaacaaggt gaaggcatcg tcgttcttgg taaaagtatc
    38821 cacgcgaaag atgcagcaca aggagtgaat gctatttctc gtctggcaat tgctctttca
    38881 gaagtattcg attttggccc aatcaatttc ttaggaaaat tagttcaaga aaatgcaact
    38941 ggtgaagctg tcgtgggcaa aacagaagat gaacaatccg gcgagctaac gatgaacttt
    39001 gcaagtttgg aaatcacacc agaacaaaca aaaatcggtg tagatatgag aatccctgtt
    39061 acattcaaga aagatgattt agtagctaaa ttaactaaaa cagcagaaaa atatgggttg
    39121 acttatgaag aatttgactt ccttgattca ttatacgtgc cactagacag tgagttagtg
    39181 aaaaatctat tggggacata tcgcgatatt actggagaca tgactgaacc gtttgtatca
    39241 ggcggcgcta cttttgctcg tacgatgaac cagtgtgtgg cattcggtgc gatgttccct
    39301 gatactcctg actttatgca ccaggcgaat gaacgatggg aattaagtag tatgtataaa
    39361 gcaatggaaa tttatgcaga agctgtgtat cgtttatgtg ctaaataaaa ataaacatac
    39421 aaaaagggcg gatattcaaa tggatattcc gtcttttttt atttgatgct ggaaagtttg
    39481 catttctctc ttattttttt taaggtataa tcatacttga ttaagaaaaa ggggtcactc
    39541 agatagaaaa ggtgggagaa agtgaaaata tttagacgaa tactaattgg cttagtcatc
    39601 attttaggat tactcggtgg agccggttat tactatatac agcaaaatac gtacgaacca
    39661 tcgtcagaag cgcaagtgtt ggtaaaagaa ggaaaaaaca aagaagatta cttatttttt
    39721 gaaggagaga aaaagaatca tggaaattct ccattactta ttttttatcc tggtgctctc
    39781 gtagagccag aaagttatag tatttgggcg caagatttag caaaaaacgg cttttcagtc
    39841 gctattgtca aaatgccact tgatctagca gtgatgggcg gaaacaaagc ggataaggtc
    39901 aaagaagatt ttccaaattc cgattatgtg atcggtggcc attcattagg tggcgtaatg
    39961 gcaagtcgtt atgtcaataa gaatcattca gacaaacatt taaaaggggt attcttcatg
    40021 gcaagttatc ccgacaaaaa aggaacattg acagaatcag ctgttcctgt tttatcgatc
    40081 acggcgacaa aagataaagt attgaatcag gaagcttatc aagaagctaa gcagtattta
    40141 ccggaaagaa cagagtatgt atcaatcaat ggcggaaatc atggaggttt tggcagctat
    40201 ggtgagcaaa aaggagatgg agaggcagaa atttctaacg cagaccagca aactgtgatc
    40261 gtcaatacat tggaaaactg gctgaaaaag ttgaaataat ataaatgggt cctatagggt
    40321 ccgtgaaaaa actagtcaat taactataga tagcaaatga atctttcaaa ataagtcggg
    40381 atttcacgga acaagagaaa cagatcccgg cttattttca agtttagaga acttagtgta
    40441 cgtttttaaa gtttcgttta tatttatatt ttattttcta ataaaatcgc tcgaactgga
    40501 catttttgat acgctttttt tacatatgtt tcttcttccg gcatcacgaa ctgttgatga
    40561 gcctctggtt cttgggcaaa taaaacgatt ccttcttcat cataatcgaa aatatctggc
    40621 gcttcgattt gacaaagacc gcatgcaata cagcgttcag gtacaagttt acatagtagt
    40681 gacatttcta accatcctaa cttaaggaga ataatatgat tgtttttatt ttatcgctat
    40741 tttcatcagg acacaagctg cggattagtt ctctttacca actccttgtg ggaaaacgaa
    40801 cgacttcagt acttatttat ggatttaccc acgaactttt gtttatccat aacagttttc
    40861 cagaattgaa gcaggataag ttttatcaaa ttttgcaaaa aattgcgcag caaggttgga
    40921 tcgaaataaa tgaaaatgaa gcaaagctaa cgcctgcagg ggctgatatg ttgtccgaac
    40981 atcaaataga acatactggt ttgcgttttg accgctatgg cagaacaggg gaaactagtt
    41041 ggcggctcat taaatttgct gttcaagtaa tttctaatct ggcaacgggt attcaagact
    41101 atcttccagc agagacaagt cctttttata ctttccaact aaaaaaatgg cttagtgaaa
    41161 gccaattgcc acgtgaaatt ttgattgata ctgcgtatga gagtttggtg cagctgtttt
    41221 cggagatacc agaagaagca gcagattttt tagctaacca attatctgga aatgagcgaa
    41281 cagggctgct tccttatcag ctggcaaaaa acaatgatga gtcagcggtt tatctgcaac
    41341 aaagcagatg tatccacctt ttgttagcac aaatcgaaaa acggccagat tcgctttggc
    41401 acgctttgat cgattcatta ttacagcaaa atttcaatca aagcatgatg atcaccaagc
    41461 agatgtttat gaatggtcag acaattgacc agattatggc aatccgtcat ttaaaaagag
    41521 gaacagtaac agatcatctt attgagtggg ccttgttttt tgatgatttt ccatacgagc
    41581 ggattctctc tgcagaaaca atcgaacgtt tggaaccaaa taaggattcc gtgcgagagt
    41641 ggcgctttag cgaatggaat gtagatggac aattagatta tggagaattt cgtttgtatc
    41701 aaatctatct attgagaaag gaagcgatac agaatgttaa taaatgaatt aaaaaaatat
    41761 tttggatttg attcatttcg tcccggccaa gaagaaatca ttcaggcgtt gcttgatgga
    41821 gaagatactc tcgctatttt gcctacagga acgggaaaaa gcctttgcta tcaattgacg
    41881 gggtttttaa tggaaggtct ggtcatcatc gtgtcaccgc tgctttctct aatggaagat
    41941 caagtgacgc aattacaaaa acgaggggag aaacgagtag ctgctttcaa tagtttactt
    42001 tcgcaatctg aaagaagata cgtcctccgt catctatcgc aatataaatt tttgtttttg
    42061 agtcccgaaa tgttgactaa tccttctctt ttagagcatc tgaaaaaaca aaatattgcg
    42121 ttatatgtag tagatgaggc tcattgcgtg tctcaatggg gagttgattt ccgtccagaa
    42181 tatcaacagt taggaaaaat ccgaaagctt ttaggaaacc cggtcacact ggcactaacg
    42241 gcgacagcaa cagatttagt agcaagagat atacgtcatg tactatttga tcgaccacca
    42301 aaagagatca gacaatcagt caatcggcaa aatatctcgc tttttgttcg gaaaacgcag
    42361 caaaaagaac aagaacttga acacttcatg gaaagagcac aaggagcggc aatcatttat
    42421 tgtgcaacaa aaaaagaagt cgaacgattg tatcatttat tcagggaacg ttttacagtt
    42481 ggatactatc acggtggtct tgatgcagct caaagaagac aacttcaaca gcagtttgta
    42541 aaaaatcagc tgcagtttct cattgctacg aatgcatttg gtatggggat cgataaatct
    42601 gatatccgtt atgtgatcca ttatgatttg ccagacagtc tagaaaacta tgtacaagaa
    42661 atcggtcgag caggcagaga tcaggaagaa agtgcagcaa ttctttttta tcaaagtggg
    42721 gatgaacgta tccattattt ctttaatcaa ctttctagag aacagcgaca aagttttgag
    42781 ctttatttgg aatatgccac agagcaagct ccctttgatg agctgcaaaa aaaatggatg
    42841 gagctgatcc agcagtctga aaaaccagaa aattgggtgg aacggctcaa acatcaggaa
    42901 aaagaaaaag aattccgcct gcagcagatg cttcgctata ttaatgaaga aaactgccgc
    42961 agaaaattca tcttagctta ttttggcgaa aaactctccg aaaagccgca gaattgttgt
    43021 gatattgatg gagcaaaagc agaacttctg ccaaagagga agcttttttc taagactgaa
    43081 caacttcatt gggaaacgat tttactaaat ttatttaaaa aaaacatgga acattgattt
    43141 tttaggacaa gacacttttt cctatggtat aatacacacg atagagttta ggaggtaaaa
    43201 cagtgagcag aaaagataga catcaatcta cagaaaataa cgaaacacaa gaaccatggg
    43261 aacaaccaat ctacgataca gatgatgaag tttcatcaag aagttctcag agacaacaaa
    43321 agaaaggaaa tacattattt ctttcgctat ttttgatttt actggtctta tgtatcgcta
    43381 ttccagcagg tgcttatttc tggatcagaa atggatcaag caacaatgca acagcagcaa
    43441 cttcttctga aacttcgaca tcagtagtag aaagttctac aagtacaaca aaagaggagt
    43501 caactgagac ttcaacagta gaatctagta caatcgcgga agaatctact gtgtctcaag
    43561 atacaggtgt aacgtcagaa agcagtattg cagcagagac aacaccgact tcctcaagtg
    43621 aggcaactta tcaagcaggg gattcaagca gcaatgcagc agaaacaaca ccttcttcat
    43681 cagctgcagc cggttcaaca acgactgttc aagcaggcga aggtccaaaa caagtggctg
    43741 agcgtgcagg gatcaccact gatcaattgt ttgaattgaa tggcttagat ccaaacaact
    43801 ttatgcttta ccctggtcaa gaattaagaa ttaaataata atagccgcgc tttgcggctt
    43861 tttttttgag aaaaaggagt tatatcatga cacaaatcag tattgcaatc gacgggcctg
    43921 cttcatctgg gaaaagtacc gttgccaaga tattagcaaa agaattgaat tatatttata
    43981 cagataccgg tgccatgtat cgcgcagtaa cttatttagc tattacgaac aatgtatcct
    44041 tttcagatga agaagggcta gtttcgctga tcaaaaagta tcctatctct ttttcgcaac
    44101 aagaagatgg acagcatgta ttcatagcat caaaagatgt gactttagaa atccgtcaac
    44161 cagatgtcac aaaagctgtt tctgaagtct ctgcacatgg aaaagtcaga gaagaattag
    44221 ttgctatcca acgaaaaata ggggaacgcg gtggcgttgt catggacgga agagacatcg
    44281 ggacagctgt attaccgaat gcagaagtga aaatcttttt agtagcaagc gtaagtgagc
    44341 gtgcagaaag acgtttcaaa gagaaccaag aaaaaggaat tccgactgat cttgaaacac
    44401 tgacgaaaga gatacaggaa agagaccatt atgattcaac aagggacgtc tctccgctga
    44461 agcaagcaga ggatgctgtt cgtatcgata cgactggcaa atcgattcca gaagtcgttg
    44521 cagcaataaa agctgttgtt ttgaaaaaag gttatcttct tttttaaatt aagaaaaaaa
    44581 atgaaaaaca attgtttttt caaggaaaac gaacgaaatc gctttatttt ttttgaataa
    44641 tcaattaaac taaaaacagt accgtactta cggcagaatg ttggttagga ggacaagtgt
    44701 tatgacagaa tttgatcaaa atcaagagaa caaccagtca atggaagacg caatgaaaag
    44761 cgtacaagaa gtaaaagttg gcgatgttgt taagggcgaa gttttagtca ttgaagacaa
    44821 acaagtgatt gtcggaatcg aaggggctgg agttgaagga gtcgttccgg caaaagaatt
    44881 atcaactttg ccggtagaag atatcagcga agtagtaaaa gtcggtgatg tattagactt
    44941 agtcgttatc agtacgatcg gcaaagataa agaaaacggc agctacctac tttcaaaacg
    45001 ccgtttagat gctaaaaaag tttgggaaga tatcgaaaga gatttccaag agggcaaggt
    45061 gatcgaagca cctgttacaa atgttgtcaa aggcggttta gtcgttgacg tcggtgtacg
    45121 tggttttgtc cctgcatcaa tggtggaaga tcattttgtt gcagactttt cagactataa
    45181 aggcaaaaca ttgagcttta aaatcatcga aatcgaacca tcagaaaatc gtttgatctt
    45241 atcgcataaa gcggtagtgg aaaacgaaag agaagaaaag aaaaaagaaa ttctttcaac
    45301 tatccatgag ggagatgttc tcagtggacg tgttgcacgt cttactgatt ttggcgcatt
    45361 tgtcgaccta ggcggaattg acggtctagt ccatgtctca gagatttcac atagtcatgt
    45421 agcaaaacca agtgatgtat tggctgtcaa tgatgatgta aatgtcaaag tgttgtctat
    45481 caatcctgaa caagaacgtg tttccttgtc catcaaagac acacagcctg gaccttggtc
    45541 agaaatcgat acaaaagctc cagttggcac agtattagac ggaacagtta aacgtttgac
    45601 aagcttcggt gcctttgttg aagtgttccc tggagtagaa gggctagtcc atatctctca
    45661 aatttcacat aaacatattg cgacaccaca tgaagtgctt catgaaggtg acgaagtgaa
    45721 agtgaaagta ttagagatcc atccagaaga acatcgtatt gcattgagta tcaaagcttt
    45781 agaagaaaaa ccagcttcag aagaagaacc aaaaaatgtt gagtcttatg aattaccaga
    45841 agaaaatact ggatttacta tgggagacat tttaggtgat gcgttgaaag aagaagtaga
    45901 agaaaaagca accgatgatt ctgaaaaata attgattttg tatctgtgac tgagatgaaa
    45961 gcagctgctt ttgtctcagt ttttcttttg aaaagcgatt agtatttcaa taaggaataa
    46021 aaatacttcg tttaattgac aatctatgct tgaaagttgg caatgcattc gttaaactaa
    46081 gtgagattgg aaacttgtga ggaggaaata atatggcaaa tccaacaatt gcaatcgttg
    46141 gccgcccgaa cgtcggtaag tctacgattt ttaaccgcat cgctggtgag cgtatctcga
    46201 ttgtcgaaga tactcctgga gtaactcgtg atcgtattta tgcaaaaggt gaatggttag
    46261 gtcgagaatt cagcgtgatc gacacaggcg gaattgatct tggcgatgaa ccctttatgg
    46321 atcagatcaa gcatcaagca gaaatcgcga ttgaagaagc agatgtaatc atttgtgtag
    46381 tcagcggacg cgaaggcgtg acagacgcag atgagatggt tgcgaaaatt ttatacagaa
    46441 gtaataaacc agtgatttta gcagtcaata aagtagataa tccagaaatg agaaacgata
    46501 tctatgaatt ttattcctta gggctaggcg atccttatcc tgtttcagga agccatggac
    46561 tagggattgg tgatatttta gatgaagcag tgaagtattt ctctgaagag actgaagaag
    46621 aggacgacga cacgattaag tttagtttga ttggtcgccc aaatgttggt aaatcctctt
    46681 tgatcaatgc gattttagga gaagaacgtg taattgtttc ggatattgaa ggaacgaccc
    46741 gagacgcaat cgacacccac ttcgtttctg aaagtggtca gaaattctta atgatcgata
    46801 cagctggtat gcgtaaacgc ggaaaagtat acgaaaatac tgaaaaatac agcgtgatgc
    46861 gtgcaatgcg tgccattgac cgttctgata tcgttttgat ggtactgaat gctgaagaag
    46921 ggattcggga acaagacaag cgagtagcgg gttacgccca tgaagctggt cgtggaatta
    46981 ttatcgtcgt gaataaatgg gatcttgtaa agaaagaaac caatacaatg cgtgatttcg
    47041 aacaagagat tcgtgatgaa ttccggtatc ttgattacgc tccaatcgtt tttgtttctg
    47101 cagtgactaa acaacggttg gaacgtctgc ctgaaatgat cgaacaagta agtatgaacc
    47161 agaatcttcg tatctcttcg gctgtgttga atgatatcat tatggatgct gtagcaatca
    47221 atccaacacc aacagataaa ggaaaacgcc tgaagatttt ttatgctaca caagtcgctg
    47281 tcaaaccacc tacatttgtc gtttttgtca atgaagagga attgatgcat ttttcttatg
    47341 aacgtttctt agaaaatcaa atcagaaaag catttacttt tgaagggacg tccatccgaa
    47401 ttatccctcg taggagaaaa taggcatttt accatatcga cgtttttttt tcaaaagaaa
    47461 aagacagttt cgtttgttac gtttctatga ctaaaaaaag catgtgaaaa ttggtaaaaa
    47521 ccttgctatt aagcggttca tatgatatcg tgataacaga atattgaagt taatcaatat
    47581 ttctttttaa acttctggac cactcagaaa gttttaacta aatgatattt ctatcatatc
    47641 tcttgtggag gaggtgaatc tatccatggc aaacaaagca gaattaatcg aaaaagttgc
    47701 ttcagctact gatttaacta aaaaagatgc aactgcagca gtagatgctg tattttcaac
    47761 tattcaagat gctttagcta acggcgaaaa agttcaatta atcggttttg gtaactttga
    47821 agttcgcgaa cgcgcagctc gcaaaggtcg caacccacaa actggtgaag aaatcgaaat
    47881 tccagctagc aaagtacctg cattcaaacc aggtaaagca ttaaaagacg ctgttaaata
    47941 agacagttga aaaagcgctt gtggctctag ggctacaagt gcttttcttt ttatttccta
    48001 gcagaaaagg aataaataag agacagagat tccgaatccg tatgtcatac atagaaaaca
    48061 catgtatttt atttaatttt tatttatttc caagttatga ggtgagcttt gctcttatct
    48121 tttctagaat ttgcttacta atggtaaaat aaaaataaga agaggaggga tgcgtatgga
    48181 acttgattat caagtagaag atgcgttgac gaaagtgttt tacggagaaa cgctagtcag
    48241 ccagctaaga gaactttcca ttgatcagcc aatcttattt ttaacgaatc agcgctatta
    48301 tgatttgttt gcagataaga tcaacccgtt atttgcaaat caagcaaaca atgactggta
    48361 tatttgtgcc aacactcaat gcaatcattt aacagaatta aagaacctgc ttgatttcgt
    48421 caaacgctat cccgaaaatc aatccatgtt tatcatcgga tttggaaatg aagggatcat
    48481 ggatctggca ggtttttttc aaaaacatac aacccttgat tcatcactat ggatgatccc
    48541 tgtatcgatc cgttctctaa gccgagcatt aacaccgcag cggacgattt tgcagctacc
    48601 aaatcgtgta gtactgcaaa ccatcaatct gccagaacgc atcgtttatg atcaaacaat
    48661 cagcgagcgg caagtggacg gaaagcagat tgactttttg acttttatct gttgcggaat
    48721 gatttgtgat catcgttttt tgcaaaatct gtataaaaac tatccaaata aaaagcaatt
    48781 aacgactcgg ccgtttgccg ggatgatcga agaaatgatc tatttttatc aattagaggc
    48841 agaaaacctg tcaaattatg gtaaactgtt tgaacaagct ttttatctga ccgaaaatgg
    48901 ccatctactt tctgctagta tgaaaaaatt tttaggaatg atgtttcatt tcgtgtggaa
    48961 tctccaagca tatccttttg atttccaact aaaaaacttt tttgtgtggc tggacttgct
    49021 tggctatcca atccactggc tggaacagat ttcaaagatg gagtatttgg aaaaagttct
    49081 ttttttggca gaaaaaagca aaaaaatact tgttttagaa aaaataggta taatagacgg
    49141 ataccaacgt ccaaacgaaa aagaattatt gagaacaatg acggtatttc aacaaatcgt
    49201 cagtgagata agagggaatt gaaatgaata caaatagcga aaaaatgttg caagctttat
    49261 cagatgaaga cttgtcacaa gcacaaatct ttttagaaaa agcattaaaa gaagacccag
    49321 cagatatatt agctgaacta ggcgaagaat tgctggctat tggctttttg atcgaagcaa
    49381 aacagatttt tgaacaactg atcaaccaat acccagaaaa tgacggactg aacatcccgt
    49441 tagcagaaat tgcaatcgaa gataatgaga tcgacttagc ttttgaatac ctggaaaaag
    49501 tggataagca aagtgattac tatccgcaaa gcttattggc gatagctgat ttgtaccaag
    49561 ttatcggaat ccctgaagtg agtgaagcaa aattgaaaga agcagctggg ttgcttcctg
    49621 atgaaccatt gatccagttt gcgctagctg aattgtattt ttcagttgat cgtttcaaag
    49681 aggcagcagt ggtttatgaa acattgttgg caagcgggat aaatgaaatt tcaggaatct
    49741 cgatgcagga acgtttagga caatccttaa gtatgcaagg agaattcgaa gcagctatcg
    49801 cacctttaga agaagcactg aaagaagaaa ggacagatga tcgactgttc cagctagctt
    49861 ttacgaatct tcaattgaaa gaaaatcagc aggcaatcaa ctatctggaa gaacttcgag
    49921 aagttaatcc gcattatcaa tcgttatatc tatatctagg acaagctctt caagaagaag
    49981 aaatgattga agaagcacaa acagtgttag aagaaggaat caaagaaaat ccttaccaag
    50041 tcgaacttta tcacctggcc tcggaaaatg ctttccgtct gcatgataga cagcgagcag
    50101 aagaactttt actgaaagct ttagaagtag gagacaaaca agatgaaaca ttacttactt
    50161 taagcaatct ttacttggat gatgaaagat atgaagatgt gatacaagtg atcaatcaga
    50221 tggaagaaac tgctaatcct tatgcagaat ggaacctagc ccatgcttat aatgaattag
    50281 aagactttac cgttgcagct gtacactatg aacaggctta tcatgaacta tcacatgaac
    50341 cagatttctt gaaagagtat gcctttttct taagagaaga agggcagctg aaaagagcgc
    50401 aagaattatt gacttattat ttaacacttg aaccaggaga tatggaggca ctgtcattat
    50461 tagatgacct aacagagagg tgagaaaaat gctaattgat gtacgggaaa agaagaattt
    50521 tttaacgtgg atggtcaatc atgtatcttt tagccgaaga gaagtttttt ggatcttgaa
    50581 ttatcttgcc aatcatgaag cgattttaag caatgtccac tttgttgaag gggcgaattt
    50641 tgcggatcga ggactgcaga tcagtgatct atctgttgaa ggggaaccca tgaaattgtt
    50701 tttgcaggga aaagaattta cggacacaga ccagattttt catgaaatcc gtttgaattg
    50761 gaaaaagcct ttatatgtgg aatgtatatt cgaaaatgct tggcagacaa gagaatatct
    50821 atctatctta gaagacaatc cattttcttc ttgggattct tcagtcagtg aagaagatac
    50881 tgaattaatt gaacgatatt ttcaagaaaa agaagaagag gcaaaactaa aaatgcttta
    50941 tgctcaaatc gatcaagcac tagaagatgg agatagggat gcttttttga aattatctga
    51001 tgaagttaac cgaaagatcc ttttgcagac acaaaagaaa agtaaccggg taagtgaaca
    51061 taacaattag ttaaaaatag tatagtacta aaaaagacaa agatcagctg gatgtggaat
//
LOCUS       DANKEF010000018        47694 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000018 DANKEF010000000
VERSION     DANKEF010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47694
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.018"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            156..1862
                     /gene="efbA"
                     /locus_tag="PWZ48_001141"
     CDS             156..1862
                     /gene="efbA"
                     /locus_tag="PWZ48_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HDL2260813.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2020..2922)
                     /locus_tag="PWZ48_001142"
     CDS             complement(2020..2922)
                     /locus_tag="PWZ48_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2260814.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2919..3611)
                     /locus_tag="PWZ48_001143"
     CDS             complement(2919..3611)
                     /locus_tag="PWZ48_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HDL2260815.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3654..4295)
                     /locus_tag="PWZ48_001144"
     CDS             complement(3654..4295)
                     /locus_tag="PWZ48_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2260816.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4333..4647)
                     /locus_tag="PWZ48_001145"
     CDS             complement(4333..4647)
                     /locus_tag="PWZ48_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260817.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4635..5987)
                     /locus_tag="PWZ48_001146"
                     /pseudo
     CDS             complement(4635..5987)
                     /locus_tag="PWZ48_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5984..6916)
                     /locus_tag="PWZ48_001147"
     CDS             complement(5984..6916)
                     /locus_tag="PWZ48_001147"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HDL2260818.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6913..7689)
                     /locus_tag="PWZ48_001148"
     CDS             complement(6913..7689)
                     /locus_tag="PWZ48_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HDL2260819.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PWZ48_001149"
     CDS             complement(7743..10928)
                     /gene="carB"
                     /locus_tag="PWZ48_001149"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDL2260820.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PWZ48_001150"
     CDS             complement(10933..12009)
                     /gene="carA"
                     /locus_tag="PWZ48_001150"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDL2260821.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12014..13294)
                     /locus_tag="PWZ48_001151"
     CDS             complement(12014..13294)
                     /locus_tag="PWZ48_001151"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDL2260822.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13313..14239)
                     /locus_tag="PWZ48_001152"
     CDS             complement(13313..14239)
                     /locus_tag="PWZ48_001152"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HDL2260823.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14252..15535)
                     /locus_tag="PWZ48_001153"
     CDS             complement(14252..15535)
                     /locus_tag="PWZ48_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDL2260824.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PWZ48_001154"
     CDS             complement(15549..16088)
                     /gene="pyrR"
                     /locus_tag="PWZ48_001154"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HDL2260825.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16323..17234)
                     /locus_tag="PWZ48_001155"
     CDS             complement(16323..17234)
                     /locus_tag="PWZ48_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2260826.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17231..17695)
                     /locus_tag="PWZ48_001156"
     CDS             complement(17231..17695)
                     /locus_tag="PWZ48_001156"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDL2260827.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17700..18398)
                     /locus_tag="PWZ48_001157"
     CDS             complement(17700..18398)
                     /locus_tag="PWZ48_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2260828.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18385..18771)
                     /locus_tag="PWZ48_001158"
     CDS             complement(18385..18771)
                     /locus_tag="PWZ48_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260829.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18918..20588)
                     /locus_tag="PWZ48_001159"
     CDS             complement(18918..20588)
                     /locus_tag="PWZ48_001159"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HDL2260830.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20771..21004
                     /locus_tag="PWZ48_001160"
     CDS             20771..21004
                     /locus_tag="PWZ48_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HDL2260831.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21103..21534)
                     /locus_tag="PWZ48_001161"
     CDS             complement(21103..21534)
                     /locus_tag="PWZ48_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HDL2260832.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21613..22026
                     /locus_tag="PWZ48_001162"
     CDS             21613..22026
                     /locus_tag="PWZ48_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HDL2260833.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            complement(22064..23212)
                     /locus_tag="PWZ48_001163"
     CDS             complement(22064..23212)
                     /locus_tag="PWZ48_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2260834.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(23334..23930)
                     /locus_tag="PWZ48_001164"
     CDS             complement(23334..23930)
                     /locus_tag="PWZ48_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2260835.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PWZ48_001165"
     CDS             complement(24130..26772)
                     /gene="alaS"
                     /locus_tag="PWZ48_001165"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDL2260836.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            27160..27591
                     /locus_tag="PWZ48_001166"
     CDS             27160..27591
                     /locus_tag="PWZ48_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260837.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(27636..28985)
                     /locus_tag="PWZ48_001167"
     CDS             complement(27636..28985)
                     /locus_tag="PWZ48_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2260838.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(29055..30218)
                     /locus_tag="PWZ48_001168"
     CDS             complement(29055..30218)
                     /locus_tag="PWZ48_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2260839.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            30347..30670
                     /locus_tag="PWZ48_001169"
     CDS             30347..30670
                     /locus_tag="PWZ48_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260840.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(30853..32202)
                     /locus_tag="PWZ48_001170"
     CDS             complement(30853..32202)
                     /locus_tag="PWZ48_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2260841.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVIAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(32271..33224)
                     /locus_tag="PWZ48_001171"
     CDS             complement(32271..33224)
                     /locus_tag="PWZ48_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDL2260842.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(33326..34516)
                     /locus_tag="PWZ48_001172"
     CDS             complement(33326..34516)
                     /locus_tag="PWZ48_001172"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDL2260843.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            complement(35161..35844)
                     /locus_tag="PWZ48_001173"
     CDS             complement(35161..35844)
                     /locus_tag="PWZ48_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2260844.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            complement(35925..37421)
                     /locus_tag="PWZ48_001174"
     CDS             complement(35925..37421)
                     /locus_tag="PWZ48_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HDL2260845.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            complement(37467..38633)
                     /locus_tag="PWZ48_001175"
     CDS             complement(37467..38633)
                     /locus_tag="PWZ48_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HDL2260846.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            complement(38842..39209)
                     /gene="rnpB"
                     /locus_tag="PWZ48_001176"
     ncRNA           complement(38842..39209)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PWZ48_001176"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            complement(39231..39650)
                     /gene="gpsB"
                     /locus_tag="PWZ48_001177"
     CDS             complement(39231..39650)
                     /gene="gpsB"
                     /locus_tag="PWZ48_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HDL2260847.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            complement(39740..40278)
                     /locus_tag="PWZ48_001178"
                     /pseudo
     CDS             complement(39740..40278)
                     /locus_tag="PWZ48_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
     gene            40342..40989
                     /gene="recU"
                     /locus_tag="PWZ48_001179"
     CDS             40342..40989
                     /gene="recU"
                     /locus_tag="PWZ48_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HDL2260848.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            40979..43360
                     /locus_tag="PWZ48_001180"
     CDS             40979..43360
                     /locus_tag="PWZ48_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2260849.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            43657..44190
                     /locus_tag="PWZ48_001181"
     CDS             43657..44190
                     /locus_tag="PWZ48_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260850.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(44316..44993)
                     /gene="nth"
                     /locus_tag="PWZ48_001182"
     CDS             complement(44316..44993)
                     /gene="nth"
                     /locus_tag="PWZ48_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDL2260851.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(45000..45698)
                     /locus_tag="PWZ48_001183"
     CDS             complement(45000..45698)
                     /locus_tag="PWZ48_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2260852.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            45966..47150
                     /locus_tag="PWZ48_001184"
     CDS             45966..47150
                     /locus_tag="PWZ48_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HDL2260853.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(47156..47599)
                     /locus_tag="PWZ48_001185"
     CDS             complement(47156..47599)
                     /locus_tag="PWZ48_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2260854.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
BASE COUNT        13555 a        10270 c         8350 g        15519 t
ORIGIN      
        1 acctaaaaat ttggatttca acgatagaaa cactgttttt tatgtaatta gtccaagatt
       61 tttctagact tttgctttac tattcggcca aattggtttt tcttcttttt ttcgttataa
      121 tgaatatact tatgaaaaga aatgaggaat gaaccatgtc ctttgatggt gtatttaccc
      181 acgcgatggt caacgaattg cgtgaaacgt tactatctgg ccggatctcg aaaatccatc
      241 agccttatga aaatgaaatc gtgctggtga ttcgttcacg cggcaaaaac catcgattat
      301 tattatctgc gcacccaagt tatgctcgtg tacagattac ccaaatcgat tatcagaatc
      361 ctgataatcc gcctaacttc gtgatgatgc ttcgaaaata tttggatggc gctattttag
      421 aagacatcga acaaatcgaa aatgatcgag tgatccattt ccactttgct aaacgaaacg
      481 agttgggtga tctacaaaat atcatattga tcgttgaatt aatgggacgt catagtacga
      541 tcgtcttagt taatcgagaa actggaaaaa tactggatgc gatcaaacac attggaagct
      601 ctcaaaacac ttatcgttct ttgcttcctg gtgtagaata tgttgctccg ccaaaacaag
      661 aggtcttgaa cccatttagc tccgagaaag aaaaaatctt tcagcggctt tcccaaactg
      721 aactagatcc aaaagcaatc caacgtcagt ttcagggaat cggtttcgat atcgctcaag
      781 agctgaccaa aagattactg gaacgtccta atgaaaagat ggttgtatgg gacgaatttt
      841 tctcagccat cagccatcag cctattccta ctttttacga aacggaaaat aaagattttt
      901 tcacaccgat tgcttatcag gtattgtccg aacaggcatc tgctgtaaca gcttatccaa
      961 cactcagtca attattagat agctattatc acgaaaaagc agagaaagat cgggcaaaac
     1021 aacaaggcgg agaactgatt cgaaaaatcg aaaatgaact aaaacgaaat aaaaataaat
     1081 tgaaaaaacg tgaacaaaca ctcaaagaat ccgaaaacgc tgagaattac cgtagaaatg
     1141 gcgaactact tactactttt ctgacacaag taccacgcgg cgcaaaagaa gttgttttac
     1201 ctaattatta tgaagaagat cggccaatca aaatagcact agatccggca ttgacaccaa
     1261 atcaaaatgc acagaaatat ttccatcgat accaaaagct gaagaacgct gttaagctga
     1321 ttggcgaaca aatccaagaa gccaaagatg aaatccaata tcttgaatct gtcttatctc
     1381 aactggaaat tgccggccca atggatatcg aagcaattaa agaagaattg acttcagaag
     1441 gatacctgaa aaagaagaac ttaaaaaaac aaaaacgtaa gaaaccatct cagcctgatc
     1501 aatacttttc ttctgacgga acgctcattc ttgttggtaa aaacaatctt caaaatgacc
     1561 agctgacaat gaagacagct aaaaaaaccg actattggct tcatgcaaaa aatatacccg
     1621 gttcgcacgt catcattaaa agtgacaagc cctcagatga aacaattaca gaagctgccg
     1681 agttagctgc ctatttttca aaataccgat actctgccca agtgccggtt gatcttgtac
     1741 aggtaaagca tatccgaaaa ccaaatggcg ccaagcctgg ttatgttatt tatgaaaacc
     1801 aaaagacgat tatcgtgaca ccagaagaag aaaaaattac agctatgaaa caaaacaggt
     1861 aatcaatgag tgtttctaga atcatatttg cgtactttcc cacaacagca attagttata
     1921 aattgtttgc tacaaaaaga ggagttgtga caaaattctg tcacaacccc tcttttttga
     1981 tcaacggtgt tcaataaaca gcgttctcat ctgcttttct tagctctgct ggtccagtaa
     2041 ccgagtaagc tctttgataa atctttgagc gatttcactg agcggacgtt tcgccgaata
     2101 aatgatataa aattcattta gttcgccttc tgccaaaggg attaaccgca agtgctcgcg
     2161 aatccctcca atcacaatac cagatcctga agcgtaagca tcagtttcaa gaacgatatt
     2221 catcaaagta cctcgatcac tcgtataaat cactgtttct tgatcaggga tctcgataag
     2281 gtcttcataa aaatacggga aattatttcc atcctgtgta aaacgaactt gaggataaga
     2341 taacaattct tctttggtga tctctgcgtg ttcagctaat ggatgtcctt gccttaaaaa
     2401 aatatgcgtt ttgaattttc caatgctgcg gccacttagc tctccttgtt gtaaatatct
     2461 tctgatacca gcttgattat gctcattcat ataaataatg ccgatctcac tgtgctgctc
     2521 tttgacttct tcgatcactc tcgctgttgt cgtctccatg atccgaaatt gtttgatctc
     2581 gtctttgaat tcctcgagta gctgtgcggt caaaggacct aagaaatcat aatgctgggc
     2641 agcgatcgaa aaattgtctc tcttttcact cgttagatag tgagtttcta atgtatcaag
     2701 ctggaccaat attttctcgg cacgatttaa gaagtcttgc ccttcttctg ttaaatatgc
     2761 ccctcgattc gttcgagtaa acagcgtgat ccctaactcg gtttctaatt ctttgatacc
     2821 agctgaaaga cttggctggg agacaaacat tttctttgcc gcttctctga aactaccatt
     2881 gtttgctaca gcgaccacgt atttcatctg ctgaatattc attgctcaaa ctcctcgaaa
     2941 taataaattt ctcttccatt caagggctgg attggtcggt tgttcggtcg agcatactga
     3001 ccatcgaagg ggagaagaaa ctcttggttc agttttccta catgatccat tacaaaccat
     3061 tggattggcc cgctggtagg cggagttgtc aatgagccaa tataatggaa atggcttttt
     3121 tgctccggaa agaacacatc cggatttacc caatgctcat gtttttctaa gttccaactt
     3181 tccccatttt cttgattata cagccaacct tcctgtacca ttagctggta catgacaccc
     3241 acaactagat tctctttttt gctgttcata tgaacaaaat gaaattcaat atcttcctgt
     3301 tttccgttga tgatatgttc agaaggcaag tggaaatgaa tatctgtcaa ataatagcgt
     3361 tctttcttga actctacata actgagttgg tcaaaaggta ctaaatgaat cgtgtttttg
     3421 aattccttgt cagtaaaccg ttgcttttga taatgaaagg aaagcgtgtc tccttctatt
     3481 ttggtttctt cccccttggt caaatgaata ggcgactgca atggaaattc cgcacctctc
     3541 gcaaaccaat cacataaact cgcccaattt tccggacctt tgtctccaga atagccccat
     3601 tcgacatcca tattcttttt caactatact tccccctgca tctcatatca tttttatttt
     3661 tcttctttct ttacaatcgt tgagacggtt tctaaaaacg cctcttttcc ataaaattcc
     3721 tggttcaata gttccggaaa tatctcttta aagaaatact gaacagaagg ccgatgttca
     3781 aagcttttaa tcgtagtcac tgcctcaatg aacatctcct tataaacttt ttccgggtta
     3841 tttttgatga tttcatcaaa agattcataa agaaggtcaa ccttatctga aatggctaat
     3901 agttctcctt ctacggtttc atcttttcct tcgaacaacc gacggcgata aacttcttgg
     3961 aactcagctg gaatctcttg gttgatgaat tcgtccgtca tcttttcttc aactgtttgc
     4021 agcatccctc ttaactcttt gcttgcgtat ttcaccggcg ttttgatatc accaataaaa
     4081 cgctctgtgt agtcatgatt caatgatttt tcatagagca gtttccaatc gatcggttgt
     4141 ccattcgctt cttcaatgtc tcctagaact tgcgcaatag atgctacacg atacgaatgt
     4201 gctgcaactg tatgctctgt atatttaaaa aatccaggtg cccttgtgat cgtctctaaa
     4261 ttgttcaatc ctaaaataaa ttcattcaac cccataaaag ttacctccct ttgagatttc
     4321 tttttccgaa tatcatatca ttaaataact gatattcgca acatttccgc tctttaccat
     4381 ttttgaaaca gctcccttac caaaaaatag agaaaatcta tatataatag aaaaaagaga
     4441 agagattatt ctcttctttc tgctaaccag tcttagaaaa atgaatttta aaaaaatcaa
     4501 aaactgatca cgatagaagt ttcttttcat acctgatgaa aagatgcttc tcgcaatcag
     4561 tttttggatt tttgctattc caattgatga ttctggagac acaaatcgga tttctctatc
     4621 ttcatcaagc gcgcttaaat ctgccaattt tcaggatctt ttttccattc ttttagcaga
     4681 gttaattctt gctcatcgat ataacgatct tctaaagctg cttcaatcaa tacagaataa
     4741 ttggtcaacg tcataagagg gatttcggct ttttcgaaat ttgcttttcc ttttggcaac
     4801 tcatacgtaa agatagccgc aacaccaagg atatccgcac cttctctttt tgctgcttca
     4861 gctgcttcca agacacttcc gcctgtagaa atcaaatcct cgatcacaac catcttctga
     4921 ccttcaaata tacgtccttc gatttggtta cctttcccat gatctttcgc cttacttcga
     4981 atatagacca ttggcaaatc cagtatttcc gctacccatg cggcatgagg gattcctgct
     5041 gttgcagttc cagcaatcac ttgtacttca gggtaagctt ctttgatttt gcttgctaat
     5101 ccttttgcga tctcttttcg gacttttgga tagctcatcg tgattcggtt atcacagtaa
     5161 attgggcttt tgattccact tgcccatgta aaaggattac ttggatttaa aaatactgct
     5221 tcaatttcta ataagtcttt tgcgatactt tgttcaacat tcattttagg ttagctccat
     5281 tctgtttgaa tcgtttgata tgctttataa ggattagttg actgagtgat tggtcgacct
     5341 ataacgataa aagttgagcc gatctttttt gcctgagaag gtgtgactac tcttgtctga
     5401 tctcctgcga ctgttccttc tggtcttata cccggagtta agcaaacaaa ttcgtctcct
     5461 gccatttctt tgatcgcttc tacttcccaa gcagaagaaa ccacaccatc taatcctgct
     5521 tttttggcac agctagcata atggatcaca cttttttcta aagaaacttc gattagttga
     5581 tcggcatgca tttcttcttc acttgtagaa gtaagctgag tgatagctag aagtagcgga
     5641 cgttttccac cttctggagt cccctcttct aatcctcgca ttgccgcctc catcatacga
     5701 ataccgccag cggcatgaac acaggtgaga tcaacaccaa gctttgccaa tccccgcatt
     5761 gctttttcga ccgtattcgg aatatcgtga agtttcagat ctaaaaatac attatgacca
     5821 gcctctttta gataacgtac gatttcaggt ccttcttggt agaacaattc cattccgatt
     5881 ttcacaaaca atttttcttc tttcgggaaa aatcgaagaa agtcttctac ctcttgccta
     5941 gttgaaaaat ctaatgcaat aattggtcgt tggtctagtt gattcatcgt tcacgttcct
     6001 ctctcacttc tttgattaat ctttccagcg attcgatgcc tagttcgctc attcgcacag
     6061 ggagctcttt aattagttta ggacagatat acggatcggt gaaattcgcg gtccctatag
     6121 ctacagcact tgctccagcc ataaacatct caagaacatc atccactgtc tgcacgccgc
     6181 ccatcccaat gattggaagg ctggatatac ttgctacttg gtgaatcaaa cgaatagcga
     6241 caggcttgat tgctggtccg gagagtccac ctgtttgatt ggctaaaatc ggttttcttg
     6301 tcttaaggtc aatccgcata ccaagaagag tattgatcat cgtaaaccca tctgcccctc
     6361 cagcttcgat tgcctgagcg attggaacga tgtctgttac atttggggac aatttgacat
     6421 agacaggaac agaagaaact ttctttactg cttgtgttaa ttgaaaagca acatcagaat
     6481 ctgttccaaa agcaattccg ccatgtttga catttgggca agaaatgttc agctcgattg
     6541 ctttcacatt tggtgcttcg ccaattttgc tacaaacttc tacatagtct tcttcacaag
     6601 caccagcaac atttgcgatg atgggtaact cattgtactc ctctaattct ggaagaagtt
     6661 tatgcataac aacgtctaga ccaggatttt gcaaaccaat tgcattcaac atgccagatg
     6721 gtgtttccgc cactcttggc gtgggattgc caaagcgggc atttggagtc gttgccttga
     6781 ccatgatcga tccaagctga ttcaaatcat agtattttcc gtattctttg ccaaaaccga
     6841 aacagccgct tgccggcatg ataggatttt ttaattctag gccaggaagt ttgaccgcta
     6901 aaggattctc attcatataa ttacctctcc tattttaaaa atcggtcctt cgtcacaaac
     6961 tttcacactt tttgttcctg tttcatcttc cggcgtatga caaacgcaag catagcaagc
     7021 ccctatccca catgccatgc gggattctaa tgataactgg acattttgat gcgaagaaaa
     7081 cagctgttcc actgtcttca ataacccgtt tgaaccacat gcaaaaacag ctgtaggttc
     7141 cgtcttcgtt cctaacaata aattaccgac atttccgtgc ataccgtatg ttccatcgtc
     7201 agtagcgatt cttgtttctc ctagacgatt aaattctttt tcataataaa cgacttcatg
     7261 tgtggcaaaa cctaaaaaat gcgttggctt aattcctttt tcgactaact gttttgataa
     7321 ctcatataga ggagggacac cgattcctcc accgattata aatacttcgt ctcctgtatg
     7381 aagattggac aaatcaaaac cattgcctaa aggacccatg acatctaaat ggtctccggc
     7441 agaaagagta gagaagactc tagtcccctc accttccacc cgataaatga tacggcaagt
     7501 atttgtttcg tggtcgatat gattcaagct aatcggtcgg cgtaatagca agtctgcacg
     7561 cggcacgaga atatgcaaga actgtcctgg ttgctgcatt tcttttacta gatcaccttt
     7621 caatgtcatt tcaaaaatct ttggtgccag ctgtttttgc tgaacgattt ccatcatttc
     7681 ttgtttcatc tgtcccctcc tgcaacggtt tttcgtcttt ttgtcttaac atgtgctcgc
     7741 cgttaaatag ggttagttga aaaagcacgg gattccatca ctcgaacgat cgcatctgct
     7801 gtgtctaaag aagtaaacaa tggaatacca tgttcaactg cttctcttcg gatgatgaaa
     7861 ccgtctttag aagcatttcc ccgatcttta tcaatcgtat tgacaaccac ttgtgtttta
     7921 ccatctctta ttaagtcgac tacatttttt tctgctgctt cctcagaaat tttcgctact
     7981 gtttcaacga ccagaccatt tttttcgaaa taagcagctg tatgtttcgt tcctatcaga
     8041 ctatagccaa tttctgtaaa acgtttagct aattctagtg cttcttcttt tgtttcgtcc
     8101 gcaatcgtaa ataatacggc tccatattca ggcaagtgta atccagaagc ttcgaatgcc
     8161 ttgtacaaag ctttgtctaa ttgtctgtca gatcccatta cttctccagt cgatttcatt
     8221 tcaggcccta agtaagtatc aactttctgc aatttcgtaa aagaaaagac aggtgcttta
     8281 acatgaactt cttttgtttc tggataaagt ccgttttcat aaccaagtgt ctttagtttc
     8341 tctcccaaaa tagctttcgt cgcaatttgt gccattggaa ttcctgtcac cttacttaag
     8401 aatggaacag tccgacttgc tcgaggattg acttcgatca cataaacctc attttcatgg
     8461 atgacaaact ggatattcat catcccgaca caattcaaac ctaatgctaa gcggatcgtg
     8521 taatcttcga tcgttttctt gatcgattca gataatgttt gaggtggata cacagccatt
     8581 gaatcccctg aatggacacc agcacgctca atatgctcca tgatccctgg gatgagtact
     8641 gtttcaccgt cacaaatcgc atccacttcg cattcaccac caagcaagta acggtccact
     8701 agtactggat gttctggaga tgctttcaca gcatgttcca tgtaatcttc taaatctcgc
     8761 tggttttcaa cgatttccat cgcacgtccg cctaatacat agcttggacg aacaagaact
     8821 ggataaccaa ttctcgaagc gataaccacg gcttcttctt tacttgtagc agtatcccct
     8881 aaaggttgag gaatatccaa ctctttcagc gcttgctcga ataaatcacg attttccgca
     8941 cgatccaagt cttcgatcgt tgtccctaag atttttacac cagcagcagt caaaggttcg
     9001 gctaagttga ttgctgtctg accaccaaat tgaacgatta ctcctatcgg ctgttctagc
     9061 tcgatcacat tcatcacatc ttcgaaggtc aatggctcaa aatataattt atccgaaata
     9121 gaaaagtcag tcgaaactgt ttcaggattg ctgttcatga tgattgcttc gtatcctgcc
     9181 tgctggattg ctttgacaga atgcacagtc gcatagtcaa attcaacccc ttgtccgata
     9241 cggataggac cagatcccag tactagaatt gagggctttt ctgatcgtga actttcattt
     9301 tcatattcat aggaactata aaaataaggt gtttttgatt cgaattccgc tgcacaagtg
     9361 tcaaccatct tatatacagg aatcaaatca ttttcttttc tcatctggcg aacggcttct
     9421 tcgtttattt cccacagctc cgccactttt ttgtctgtaa aaccgttttg tttcgctgtt
     9481 ttcaatatct tgtcatccaa cggctgctgt ttcaactgct gttcgatttc tacgatatgc
     9541 aacagtttat ctaaaaagaa taaatcgatt tttgtcaaat ccgctaattc ttctatggtg
     9601 taccctcttc taatagcttc agctacataa aacaggcgat catcttgtgc tcgaaccact
     9661 ttttggatta ggtcatcgtc cgtgatgtct gccaactcag gaagttcgat atggtgtgta
     9721 ccgatttcta aagaacgcac agctttcaat aaagactctt cgatattccg accgatagcc
     9781 atcacttcac cagttgcttt catctgcgtg cctaaaagcc gttctccgtt ttcaaactta
     9841 tcaaacggcc agcgaggaat tttagctaca acataatcta atgcaggttc aaattctgca
     9901 aaagtcgttc ctgtaacagg atttttcatt tcgtctaatg ttaatccaag cgctatttta
     9961 gcagctagct tagcgatcgg atagccagtc gccttacttg ctaacgcaga tgaacgagaa
    10021 acacgcggat tgacttcaat cacataataa ttaaaactat gtggatcaag agcgagttgg
    10081 acattacagc ctccctcgat cttaagtgct cgaataatcg aaagagaagc atcacgaagc
    10141 atttgatatt catggtcaga caatgtttga ctaggggcaa atacgataga atcacctgta
    10201 tggataccca ccgggtcaaa attttccata ttgcagacaa cgattgcatt atcagccgaa
    10261 tctctcatca cttcatattc aatttcttta aaacctgcaa tacttcgttc aatcaaacat
    10321 tgggtagcgg gggaaagact taatccattt tctgatacga tacgtaattc ttcttcattt
    10381 tcacacatac ctccaccagt tccgcctaaa gtaaaggctg gacgaacgat gacaggatag
    10441 ccgattttat ttgcaaaatc tacagcttct tctactgtat tgacgatttc agactcgggg
    10501 attggctgat tcaattcttt catcaactgt ttgaatagat cacgatcttc tgcttgatcg
    10561 atcgcagaaa gctttgttcc tagaagttct atgttcaatt cttctaaaat acctgatgct
    10621 gccagctcca ttgccatatt gaggcctgtc tgtccgccta aagttggaag gagagcatct
    10681 ggacgctctt ttcgaagaat ccttgataca aattcaagtg tgatcggttc gatatatact
    10741 tgatctgcca cttctttatc cgtcatgatc gtcgcaggat tagaatttac taaaaccact
    10801 tcatatcctt cttcttttaa ggcaaggcag gcttgtgttc ctgcgtagtc aaattctgcg
    10861 gcttgaccaa tgatgatcgg accagatcca atcaccataa ttttcttgat atctgtacgt
    10921 ttcggcatct gtttactgct cctttcctgc gtccatcatt tccataaatt catcaaataa
    10981 atgtagtgca tcatgcggac caggggcagc atctggatga aactgcacac tgaaagcggg
    11041 ataatctctg tgtttgactc cttctactgt tccgtcattt acttctacat gagttacgat
    11101 gagtttttct ggatcaatcg tggcttcatc gactgcatag ccatgatttt gagaagtaaa
    11161 atcaattctt ccggtcgcta cttctcgtac cgggtgattt aatcctcgat gtccgaattt
    11221 cattttatag gtatctgcac cgttggctaa agcaaataac tggtgaccga gacaaatacc
    11281 aaaaatcggt acttttcctt ggattttttt gatcatttcg attgcttctg gcacatcttt
    11341 tggatcacca ggtccattcg tcagcatgac tccgtctggt gatagcgcta aaatcgtctc
    11401 ggcatctgtg ttatagggaa gtactgtgag gttacagttt cttttactca attctcgtaa
    11461 aatactatgt ttcaagccga agtcaatgac aatgactttc cgacctgttc ctggacttgg
    11521 ataaggtttg ttagttgaga cttgctgcac ttgattggtt gctagaacgg ttgctttcag
    11581 ctgatcaaat gcatgttcga aggaatctcc agcatcaacg atactagctt tcatcgttcc
    11641 gacttcacgg attttgcgag taagggcgcg cgtatcgata cctgatattc caggaattcc
    11701 tttttgtttc aaaaatgaat ctagcgtcat ttggtttcgc cagtttgaag ctacacgtgc
    11761 atgttctttt actactactc ctttgcatgt tggagaaatc gattcataat catctctatt
    11821 taccccataa ttacccacca taggataggt aaatgtaatg atttgaccgt taaaactttg
    11881 atcagtgata gtttcttgat aaccagtcat acttgttgta aaaacgattt ctccgaagac
    11941 actagcatct gcaccaaaag cctttccttc gaaaactgtt ccatcttcta atataagcaa
    12001 acgttccata cttttattct cccttctgcc acacaagttt tccgttgaca aaagtcattt
    12061 gtgtttcgcc ttttactttc cagccgataa aaggtgtatt ctcccctttt gataaaaagt
    12121 cttctttatc gatcgtgcaa gtttgtgtaa tatcaaaaac tgctacatca gcgggggcac
    12181 caaccgttag cgttcctgca ttcaaaccaa aaatctcagc cggtttaact gccaaccaat
    12241 tgatcacttg ttccaacgtg aatcgaccag tttctacaaa atgagtatag atcagttgaa
    12301 acgctgtttc gcttcctacg ataccaaatg gtgatttcat gaaactttgg cttttttctt
    12361 ctaatccatg gggggcatgg tctgtagcga tgcaatcgat cgtaccgtca agcaagcctt
    12421 cgatcaaagc ttctctgtcc tcccgtcctc ttagcggcgg gttcattttc caaaagccga
    12481 aatcttcggg aatatcttcg tcgatcaaaa taagatgatg gggtgaaact tctgctgtta
    12541 cgtggattcc tgcttttttt gcgtctctga ttacacggac actttcttta gtagaaacat
    12601 ggcaaacatg gtaatgaaca ccagttgctt cagctagcaa cagatctcta gcaatttgag
    12661 aagactcggt tacgcttaag atcccgggaa gaccaagttc ttctgccttc ttgccagcgt
    12721 gcataacgcc gccaaataat aaagattcat cttctgtatg ggctactagg gctttattgt
    12781 tttttgcagc ttctttcata gccagataca tcgtcccagc tgtttggaca cctacaccgt
    12841 catttgtgaa agcaaaagca ccttcttcaa tcagtgcttc ttgatcaact aatttctcac
    12901 ttcgcagatt ttctgtgatt ggcgcatact gcaatacctt cacaactgca tcttttctaa
    12961 tgatgtcata tacttgtctc agcttttctg ccgtatcagg tacaggattt aaattcggca
    13021 tcgcgcatac agtagtgaaa ccgccacgag cagctgagcg agtcccagct tcgatcgtct
    13081 ccttgtatgt aaatcctggc tctctcaaat gaacatggac gtccactaaa ccaggtgtga
    13141 tcaattgtcc ctttgcatca aacacttcat caaattcggc ttctgagaat cctgtaccga
    13201 ttgctttgat tcgcccatct tctatccaaa tttctgctgg tgtcgtcata ttttttcgtg
    13261 tattgatctg accgttttta atgagtgttt tcattttttc atcctcctat aattacgctt
    13321 ttccatttag cactgcttct aagattgcca ttctgacgaa tactccattg ctcatttgct
    13381 gaacgatccg cgactgtaat ccttccacta atgaatcagc taattctaca tcacggttaa
    13441 ctggtgctgg gtgcataatg attgcatgtt tttgcaactt cttcgcacgc tcaactgtta
    13501 acccatgttc ctgatggtag ccttcttttg agaaactttc ttttccatca tggcgttcgt
    13561 gttgcacccg aagcatcatc atgacatcaa cttgatctag tagttcatcc aatggcatat
    13621 aatgtccgta cacttcgaat tcatcgtcgt accattctct aggacctgag aagaatactt
    13681 gtgcgcctag acgttttaac atctgcatat tggatttagc tacgcgagag tgagtgatat
    13741 cacctacgat ggcaactttc agaccttcaa aatgaccaaa ttcttcataa atggtcatca
    13801 aatcaagcaa gcattgggtt ggatgctgtc cgcttccgtc tcctccattg ataatcgaac
    13861 actggattgt tttactttgt atcagttcgt cataatagtt ctcatcacca tgacggataa
    13921 ccgctacgtc tacgcctaat gctgacatcg tcagtactgt atcgtatagt gtttcccctt
    13981 tttggacaga gcttgtgctt gcttcaaatt caatcacttc tagccctagt tttttctccg
    14041 ctacatcgaa gcttttatgt gtgcgagtac tattttcgaa aaagaggttt gtagcaaagt
    14101 actggcgctt ttgcggagtc caattagcac cttgcttgaa ttcctgccct ctgcggatca
    14161 agcccataac ttcttgatca gtaagtgcct caacggtcaa taaatgcttt aagctgatac
    14221 gttctgattt gatgatcata atgtttcctc cttagttttc ttcgcttcgt gaagtttctg
    14281 gtaagataag atttaaaatg atgccaagta cagttgctaa tgccattgcc gacaatgtaa
    14341 atgtgcctac ttcaaagacg agtccgccaa ttcctactac taagataact gaagcaatca
    14401 acagattttt ctttttatca aaatcgattt tgttctcgat taaaatcttc attccacttg
    14461 cggcaattac tccaaataag ataaaactga ttcctgagat gactggtcca gggatactta
    14521 aaatcaaagc gcttaatttt cctacgaatc ctaaaatgac tgcaaatact gcagctccac
    14581 cgatcacaaa cacgctatgg acacgtgtga tagccagaac accaatgttt tcaccgtaac
    14641 ttgttacagg aggacctccg acaaaggctg ccacgatttg tgctaatcca tcacctgtca
    14701 acgtcttatg aagacccggt tcttcaaaga aattacgttt agttagttta ttcaatacca
    14761 taagatggcc gatatgctct gtcatcgtca caaaagcgat gggtgccatt gttgtgattg
    14821 ctccaagata tagctttggt tcatattgta caaagggaat ctcaaagtta ggaacagcaa
    14881 accaggcagc gtctacgatt ggcttagtat ccacgattcc aaacagcaag gctacgatgt
    14941 atccgctaat aatacctagt aaaatcggaa tcaatccgag aaaacctctt agccacatgt
    15001 tgaagaaaat tgttaatcct aatgtgatca gcgctacagc aatatacttg aaatcatacg
    15061 tgccctgatt atacatagca tttgttgctg catttgccgc aagtcccaac ccgataacca
    15121 tgatgactgg accgacgacg attggaggca agattttatc cagccatgct gaaccgatct
    15181 ttttgatgat cagcgcaaca atcaaataga ctaatccagt tgtgactgct ccttgtgcaa
    15241 tcgctggata accatctgtt ttcatcaaaa gctgcatcgc tgcaatgaaa gcgaagctgc
    15301 ttcctaagta cgcaggtatc tttcctttag tagtaaataa gtacaccaat gtacctagtc
    15361 ctgaactgac tagcgcaata cctggatcga tacctactaa gatggggact aacacggttg
    15421 ctccgaacat cgtgaacaag tgttgtaaac ttaatcctac ccaatgtcct ggggttgggc
    15481 gatcgtggat atctaacacc acgtcatcat tatgaaatcc ttttttctcc gacatgtttc
    15541 tctctcctct aatcttcctc tttcaaaatc atgatgcgat cttgtccatc aagctcttgc
    15601 atctctacta aaatctcttc cgcccgagat gttggaatgt tcttcccaac ataatctgca
    15661 cggattggca attctcgatg cccacgatcg accaggactg ctaatgaaat ttttcttggt
    15721 ctgccaaaat ccattactgc gtccattgct gctcgaatcg ttcgtcctgt atacaaaaca
    15781 tcatcaatca aaataacttc ttttccttct aaggaaactg ggatgtcaga agaatgaagt
    15841 tccggttctt ctggattttc ttttttgtca tctcggtata aagtgatatc cagttctcct
    15901 acaggaatat cgatatcttc tagctgcttc aggcgttcgg caattcttga tgcaatgtag
    15961 attcctcgtg ttttgatacc taccaatacg atatcttgga tactatggtt tctctcgata
    16021 atctcataag taattcgagt caatgctcgc ttcatggtta cctgatctac tacttctttt
    16081 gcttgcattt tcattccccc tacttttttg cataaaaaaa ctcctgccct aagaggacag
    16141 gaggaatgtt tagactgagc gtatactgat acactgatag ctatactatg tcaatgagca
    16201 atctacttat ggcgcaccat acgtatcctt gctcccaccc ttcttagcct cacgggacta
    16261 aacttaaagg aattctatta agttttatgc tttcgttgtt aattactata ccgttatcta
    16321 aattaaaatg caatgttttt tcttaatttt tctaaagttt tttcaaaaac ttctggaaga
    16381 ggtgcttcaa agatcatctt ttcgtttgta gtcgggtgcg taaaacctaa catttgcgca
    16441 tggagaaact gaccattgcc tttcaaagtt ttcttcggtc cgtatactgg atctccagct
    16501 aacggataac cgatatactt catatgcaca cggatctggt gtgttcttcc ggtttctagt
    16561 tttaattcaa ccagagtaaa tccttcgaaa cgttccaata cagtaaaatg tgtgacagcc
    16621 gacttcccat cttctcttat cgcctgcatt ttccggtttt ccttcgaacg gccgattggc
    16681 gcattgatgg ttcctttttc gtgaggaatc tctccatgga ctaaagcaac atatttgcgc
    16741 aaagaggtct tatcttttaa ttgctgagct aatgattcgt gtgcctggtc atttttggca
    16801 accattagca gtccggaagt atctttgtcg attcggtgaa caatacctgg acgaataacg
    16861 ccgttgatcg tagaaagatc cttgatatgg tacatcagcg cattgaccat tgtgccatgc
    16921 gtatgacctg ctgaaggatg aacgaccatt ccttgaggtt tgtttatcac cgctacatct
    16981 tcatcttcgt aaacgatcgt caacgggata ttttctggga ccacatctaa tgtttctggt
    17041 tcggggatca agacagtaat ctcgtctcct tgttttactt tgtaatttgc tttgactggc
    17101 tgtccattga cagaaaccgc tccatttttc agccacagct gaatttgcga acgactgtaa
    17161 tcggtgaata aattagtcaa tactttatcg atcctaccag attcatggtg aatcgtcgtt
    17221 ttaagttctg tcattttttt ctttgatcct tttcatctaa aataatataa attaacatac
    17281 aaatcactcc acatacaagt gtcacatctg ctacgttaaa aatcggaaag ctcataaaat
    17341 ctgtctggaa catatctact acatagccaa gacgaatacg gtcgataaaa ttgccaatcg
    17401 caccagctag aatcaaactc aatccaatca tgaaccagcg attacctttt tcacgatttt
    17461 taatcatcaa tgtgagaatc acaacgactg caatgagtgt cactatagta aagaaccaca
    17521 tcttcccttc tagcatactc catgcggctc cagtgtttcg aatatgattc agtgacaaaa
    17581 tacctggtat aagatttttc gtctctccaa gcgctagatt atcagtgatc caccatttga
    17641 ccaactgatc cactcctact agcaaagcgc tgaatatcca ataaataatt aacaaaaaat
    17701 caatcctttt ctatttgttt ataaatatca cgaggtcgta cgatcccgag cagattacca
    17761 tcgggattcc catctttagt gataagtacg gcttccaaag tcccttgctc acgaaacatc
    17821 gcttcaacct cgtaaatatg tgttttagct ggaacaaaat gataatttgt gtactttccg
    17881 ttttggatga tcaaatccaa cgctcgttta tttgttaaat caaccggacc tttttggctt
    17941 tcttttgcta gccaaaaccc gatattacgt aaagtaatca atgctacaaa ttttccttta
    18001 ttgtataacg gaaattgtga ataccttttt tctgctacta tctttaacag ttcaagtaac
    18061 ggaagatctt gttcaaaacc tgttacacgt tttgcaaatc gaggcagcac ggtttcaggc
    18121 tgaatcagtt cttgttcgat cagctggatt cgcttgactg tccaatcatt tggctctgca
    18181 atgacaaaat cgactgcgat ccgatcatgg acgatcgcat tccttaactg agcaagctgt
    18241 aaaagatcat cttcgtattt tctgatcatt aattgttttc tttgagctag gcgtctgaca
    18301 agttcggtaa atcccatatt tcgtgcattt cccatctcgt ctctcatcca tttttcgatt
    18361 cgattgaaac tgattaaaaa aagatcagag tttgccataa ttttcctcct tgatcaaata
    18421 tgcccggttt tttatataga aaaagaacgc aatcaacaca ataactgcaa aaaaactgaa
    18481 aaataaattt tgtaggaaaa cggcccccaa cccagttgct agaaataaaa acggatagat
    18541 ttgaagaatc aagtaattca ttgtgatcac ataagcaggc tttgcttctt tcactgtata
    18601 aacggggctt tttagccaat aaaaggtgac ctgcatcgta aagggcactt ccagatcaag
    18661 cgttatcttt tgttcatgac tgagaacact gcgtttttca ttgttgattc ttagctgaat
    18721 cgagctaccc ataccgtaaa aaccggtttt tcttataaat gtcgttttca tcataccctc
    18781 ctattatgct ttagtatagc tcaaaataca ataaagaaaa agaatgatcc tagtgtaaac
    18841 aaaattaatt ccctatcgtc taaaaaaaga agctgtagtc aaaatacagc tccttcgatg
    18901 gtagattata atcaagctta aaacaatccc aacacttctc cgtcatttgt cacgtccata
    18961 ttcaatgcag caggtttttt aggcaaacca ggcatcgtca taacatctcc agtcaaggct
    19021 actaaaaatc ctgcccctaa ttttgggatg atttcgcgga tcgtgatggt gaaatcttcc
    19081 ggtgcaccta gcagtgaagg atcatcagaa aatgaatact gtgtttttgc catacaaatc
    19141 ggcagtttgt cccatccatt cttcgtaaac tcttctattt gtttttgcgc ttttttgctg
    19201 aaaacaacac catttcctcc gtaaattttc ttgacgattg tttctgtctt ttcttgtatc
    19261 gtttcttcta ggcggtataa tggcttgtaa ttagcttctt ttctatcaat caatcgtact
    19321 acagcttttg ctaaatcaac gccgccttct gctccatctg cccatacact cgctctcttg
    19381 gcaagaatcc cctgattttc acacaagtgc tccaacagtg tgagttcatt gtctgtatct
    19441 gttacgaatt cattgattgc gacgatcaca ggtagctgat attgttccat attacgtata
    19501 tggcgcttta aatttgcaaa acctactttc aaagcatcaa gattctcatt tttaagttca
    19561 tcttttttca ttccgccatg cattttcaat gcacggacag tggcgacaat caccacagca
    19621 tccggcgctt ttttcaagtt aggtactttg atatctaaaa atttctctcc gccaagatct
    19681 gcaccaaatc ctgcctctgt gacaacgtaa tctgctaatc tcaatgctgt cttggtagct
    19741 aaaatactat tgcagccatg agcgatattg gcaaacggtc ccccgtggac aaaagctggt
    19801 gttccgtaaa ttgtttgtac aagattcggc ttgattgcat ctttcagcag taatgccaga
    19861 gcgccttctg ctttcaagtc tccagcagtc acaggctgac ggtcaaatgt atagccgaca
    19921 acgatccttg caagacgagc ctttaaatct tcaaggtctg ccgcaagaca caagatagcc
    19981 atgatttcgc tggcaactgt gatatcaaac ccatcttctc ttggaacacc ttggattggc
    20041 ccacccaagc caacaattac tttacgcagt tcacgatcat tgagatcgac tacccgtttc
    20101 caaatcactc ggcgggcatc gatgttcaat tcatttcctt gatgaatatg attatctaaa
    20161 agagcagata atgcattatt agctgttgtg atagcatgca tgtcaccagt aaaatgcaag
    20221 ttgatatctt ccataggcaa tacctgtgca tatcctccgc ctgcagcacc acctttgatt
    20281 cccatcacag gaccaagtga gggttctcgc aatgcaataa cagatttttt attgatttta
    20341 ttgagcgcgt ctcctaaccc tacagtgatc gttgactttc cttcgccagc tggtgttgga
    20401 ttgatggaag taactaagat caaatgcccc tcagcgttat cctttgatcg attgattcca
    20461 gaaaaattga ttttcgcctt atatttccca tacagttcca aatcgtcttc cttaagatct
    20521 aatttactag ccacttcaac aatcggttct agctctgttt cttgtgaaat ttgcagatca
    20581 ttcttcattt gattacccct tttcacgaac tttattcaac aaaatatctt ctttgttaag
    20641 atatgccaat tatacctttt tttattcaga aaaaaaacat aaaattacag gtttttaatt
    20701 tatttgacgt acttataaat actctttata atgaaggcta tgatcagact ttagaaaggt
    20761 cgtgagtgct ttgaccacag gaattgtaaa atggtttgac aacaaaaaag gatacggctt
    20821 tatcagttat gatgacacag aagaaatttt tgtacatttt actgcgattg aagaagaggg
    20881 attcaaaact ttggaagaaa atcaagtggt cgaatttgag atcatcgaag ggaatcgagg
    20941 aacacaagct gcccatgtca agaaaggcaa cgttcagact accgatacta gtatgcatga
    21001 atagataaac aaatagagtg agacgctaaa cgggatgaac cgtacgtctc actcttttct
    21061 tttcctgtaa aaattcgttt ttttaggttc tgatttttaa aattagcttt ttcttttttt
    21121 cgtataagga taaggaattg attctgattt gggtccgttt ttttcaaaat gatcaaaaag
    21181 gactacttga tctttttttc gcatcatgaa aacagatggg gctttctcat cttgcaacat
    21241 catttgcacc ccagtattag ttggcgtcaa agatcgaatc tgtccgacag atatttctcg
    21301 gtctttccaa aaagcagcat aacgtataat cacagagttt gattttaaaa taaaagaacg
    21361 ccgcaaacct agaaatagaa aaacaagaaa aatacctaaa acaaaattgc ttttccaata
    21421 aggacctgtg ttttctaaag aaagaatcag actaaaaaac agaatgatca aggtcaatga
    21481 ccaatagatg atcgtatgtg ccagttcagg ctgccatcga atattttttt tcattctcta
    21541 acctctcacc gattcagtaa tgatataatc ttaccataac attagtttga acaaaaagga
    21601 gaatctcttt ttatgttaaa agtatacatt gatgcctcta caaaaggcaa ccctggtcct
    21661 agtggaggtg gaatcttgat tgtctatcaa ggtaaacaag aacagctatc tatcccgctt
    21721 tctataggga caaaccatca agcagagttc gaagtctttt taaaaacttt agaatttcta
    21781 aagaaaaacg acttacagaa tgaaacgatt ttctgttttt cagacagcaa aacactagtt
    21841 tctacggtcg acaaaaacgc aacgaagaac gagctcttcc ttccctacct tcaaagaatc
    21901 caagaacttc tatccgaatt ttctttattg atcctgcaat ggttgcccga aaacaaaaat
    21961 aaaggagcag ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact
    22021 ctttaaatta aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat
    22081 tgtgatcaaa cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta
    22141 agattctttc tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt
    22201 gcagcgtttt tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt
    22261 caagaagaat ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca
    22321 ttggatccaa gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca
    22381 gatcaataat cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg
    22441 atgcaaagcg tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat
    22501 catcgcaagc atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc
    22561 ctaatagttc gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg
    22621 ctaaagcaaa acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg
    22681 aaagcaatac agccagtgta gtaaagacat cattataact atctgttgct gtagctctca
    22741 atgtttcaga atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca
    22801 aaaagatgga aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat
    22861 ggatgatttt ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg
    22921 aaacaaataa cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg
    22981 ctggctttgc agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata
    23041 gactgttcac tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct
    23101 tagagacaaa caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt
    23161 ttgtccgctg ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta
    23221 ccccttctag tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc
    23281 ctctcttcct ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct
    23341 ggtttcaacg gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca
    23401 cctattgctt ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga
    23461 gcagagtcta caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct
    23521 aacactaaga ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt
    23581 attgatttat ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt
    23641 actctgggaa ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt
    23701 ggcatggcat aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca
    23761 tcttttgggc ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta
    23821 atgaatccta atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct
    23881 aacaagatcg cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc
    23941 ctttttattt atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga
    24001 atccgaccat ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt
    24061 tgtcttcgcc cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac
    24121 gtaaacgccc tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc
    24181 agcaggattt ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac
    24241 tttcggtgcg attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt
    24301 cgtcattgcc gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc
    24361 tgaaatattt ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac
    24421 ttttacttgt gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc
    24481 accagcttgt tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct
    24541 tacttgctgt tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac
    24601 aattgttgct acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt
    24661 acttgttaca gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt
    24721 gaagattccg atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca
    24781 gccgccaata tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc
    24841 acccatgttt ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca
    24901 gattttttcg ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa
    24961 gtgggtaaag tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg
    25021 ctcgccaagt acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat
    25081 gatccgattg cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg
    25141 tgcttttact tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac
    25201 aactgctacg atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc
    25261 catttctgca tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc
    25321 attcaaaaga gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt
    25381 gtatccaatg aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat
    25441 tgatttttca gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc
    25501 aaaaccagca tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac
    25561 cgggaagcca tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt
    25621 ttcgtttttt acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc
    25681 attgaaacgt tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac
    25741 ttcaggataa taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc
    25801 ttgttcaata cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac
    25861 ataccctctc ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg
    25921 aatatgatct gcgattactt tgtaagaaat atctgttact gggttttcac catattttac
    25981 ttgtttgctt agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt
    26041 cgttgacgca tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt
    26101 tttatgagga agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa
    26161 gttccagatt tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc
    26221 tgctaaatcg ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg
    26281 gcctgcaccg atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc
    26341 aacttcttca tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag
    26401 tttttctgga tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat
    26461 ggcttcattt ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg
    26521 gtgccgtgct gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt
    26581 tgtgattctt ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac
    26641 accagagttg atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc
    26701 aatcgtatgt cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact
    26761 taattgtttc atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag
    26821 ccaaggacga aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta
    26881 aactcgataa cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg
    26941 attagtgact attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat
    27001 catatccttt tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat
    27061 ttttattttc atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga
    27121 atcaatacat ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg
    27181 gatgaatgga tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa
    27241 gggattcctg cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta
    27301 tttcttgatc aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc
    27361 ttcaagccgg aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag
    27421 ttgcttgaag ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta
    27481 gctgaaatga cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct
    27541 tttattgaag atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag
    27601 attgtgacac gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt
    27661 ttacgagcag tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct
    27721 gcaattgccc aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc
    27781 acaagaccaa tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta
    27841 ttacgatcgt atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg
    27901 cccaattttt ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc
    27961 gttcctttta agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct
    28021 tctggaattt ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct
    28081 aaatcagtcg ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt
    28141 ctctctcttg gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag
    28201 taatcggcaa tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga
    28261 tgaccaattg tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc
    28321 agccaatttt caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga
    28381 ttttccatgt attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact
    28441 aaaaactgca gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc
    28501 atatccaatg tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc
    28561 gcttgttcat tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt
    28621 tggtgatgca gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca
    28681 cggatctccg gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct
    28741 agttccctac ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt
    28801 tcaaacaaag gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg
    28861 atattttttc cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg
    28921 aagccctttg cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta
    28981 ttcattcgtg cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa
    29041 gcgtttttgc tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca
    29101 gtatatgtgt gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc
    29161 aagatattta aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca
    29221 ctcatctgca acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac
    29281 cagcttaaac cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa
    29341 gaaattcgtt cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc
    29401 catccattgg ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac
    29461 cagatatgag aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc
    29521 aaattaataa gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat
    29581 gagacagtct ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata
    29641 aatgtaaata acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa
    29701 tcattcacat caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc
    29761 gtcaattcaa aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc
    29821 cggaaataat atcttaaata aatgccaaat ggtatgagta ataaaacatt aaaagctggt
    29881 tccaaaaata cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat
    29941 accgtgttgt ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct
    30001 gtcatttggg caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac
    30061 aataaataaa aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac
    30121 tttcgatatt cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca
    30181 atcagcgcaa aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg
    30241 tatctctctt atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct
    30301 catttctttc aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta
    30361 ctttgttttt ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag
    30421 aaaaagaact acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct
    30481 agtcttcaat cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca
    30541 tgctttgtct aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc
    30601 tggttgcttg gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa
    30661 atcaaagtaa aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca
    30721 tcgtagacaa aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca
    30781 cgtactcctc atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt
    30841 cgaatgtttt agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa
    30901 tactatcccc atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt
    30961 ccctccaatg attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa
    31021 caaatcaggt tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat
    31081 ccaaatgaca aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc
    31141 aatagcgatg actagctcta agatacctgc ggctacagga actttagttt tatcatgaag
    31201 tttagcaaag agcttgctaa aaggcaggtt gttttctttt gcaataacat aagggagacg
    31261 cattccagta agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt
    31321 gaccagtctt ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct
    31381 gtttcctgcg actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata
    31441 aacgatcatg attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt
    31501 tttcaattct cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc
    31561 tagcaagccc gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa
    31621 cagttggaag tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg
    31681 aataagcttg cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa
    31741 attgatcaac aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga
    31801 aagaccaaat agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc
    31861 tggaaaataa atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat
    31921 ccgttcgata tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc
    31981 tgttagtcct gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact
    32041 ggcggatcct gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac
    32101 tgtgcccata acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc
    32161 tatttttact tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata
    32221 ccatagccaa tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc
    32281 tctaccaatt tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat
    32341 ttcggcgaaa agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat
    32401 tctgctattg actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt
    32461 ttttgaatag atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca
    32521 tttcgatgaa ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca
    32581 tctggcatat acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct
    32641 tctttgattg ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag
    32701 gttgcataag aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca
    32761 taataataat cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga
    32821 cgctgaagag tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt
    32881 gaacttggcg taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc
    32941 ccagtcacgt tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga
    33001 gcagccaaat gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg
    33061 taatccatta catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt
    33121 atatctgtcg caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct
    33181 gaaccaattt gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat
    33241 cttttttact actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt
    33301 aaatagacga aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat
    33361 aaactgctac tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga
    33421 tcctgcctgt cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt
    33481 cgatcagctt cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa
    33541 taggtgtttc agacataccg atgttatagc ctactttttt caattcttct ttaaaataat
    33601 tagcattttc ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag
    33661 ccaatgcagc tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc
    33721 tagctttcaa ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta
    33781 tagctttcga taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga
    33841 cggttcctgc accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca
    33901 gtccatattt ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg
    33961 agaaaacacc atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct
    34021 ttgctttttt ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg
    34081 ataagcggca accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt
    34141 cttttgtcat tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga
    34201 ttgctgcttc tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca
    34261 gcgaaccatt gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa
    34321 tacatgcttt tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat
    34381 tgatcatctt ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga
    34441 tcgtattgta taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta
    34501 gttctttgga agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac
    34561 atatatcgtg tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg
    34621 attatgagaa ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact
    34681 tatatagtat gaacaattac cttttagata atgatctatt ttactctacc tgggcagata
    34741 aatacaacac aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt
    34801 aatttattta ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca
    34861 atttttaatt ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag
    34921 gactgtgaca aaagtcttgt cacagtccta ttcttccgaa taaacggtgt tctcctgcca
    34981 attccgaatc acaagttaca atattcatca aacacgagaa gctatttttt gaaattgttc
    35041 cttgtgactc ggagctgaac ggcaggctca cgacctcttg gtaaacagtg ttccagaagt
    35101 aacttttacg gaaaatgatc cgaaatatct tggagctgac cacttctgtc acaacctcca
    35161 tcattttttg ttatttttga tccaattgtc tggagtaaaa ttttcatcgt caatagacca
    35221 ttccagaaaa aattcttcta agaattcacg catgaaggtt tctcttcgtt tggcttcttc
    35281 atatccgtaa tcagtattca ttttatcaga cagcaagaac agtttttcat aaaaatgatt
    35341 gatcactgtc gtgccttttc ggtaattttc tttgtccttg aatgtttgcg gatacagttc
    35401 aggatcaaaa atcgggtgcc catgtccacc gccgaaataa gcagtacgca aaatgccgat
    35461 tgctcctaat gcttcgatac gatcagcgtc ttgaacgatc ttaccttcca gagatagttc
    35521 ggccgcttca gagagatttt tagaaaagga catattttcg ataatggaaa aaatcatcga
    35581 gatctgttcc tctactattg gcaaagagcg aaggaataca cgaagctgtt gctttgcctc
    35641 gttctcattt ttaacgactt tatcatcaat cgtatcatgt aggtaagctg ctgcaagcgt
    35701 cacaaagcgg tctgcctgcg gttccgtctc caaaattttt tcagctaact tgacaactct
    35761 tttggtatga tcgctcccat gtcctgtttg atcatgcgct aattggtcca tactataatc
    35821 agcgatcact tgtaatttat ccattcgttc cacttctttc ttaagtaaat agctgaaaac
    35881 atctatagat tagatgaatt tcagctatct ttgtaaattg tattttattt tacctgatat
    35941 acattaaaat acaggtcttt catatagtcg atcaggtatt gcggattaag cggttcatgt
    36001 gttgcatcca tgattaattg atttggtttt ctagatgcac catattgatg gatatgctct
    36061 gacatccagt ttttgattgg agaataatcg tctgacgcta gaacattttc cacatctatt
    36121 tcttgctgca aagaatgaag taattgtgcc gcatacatgt accctaatgc ataagaggga
    36181 aaataaccaa agcttgcacc tgaccaatga acgtcttgta ggatgccttc cacatcattt
    36241 gttggtcgta ttcccagata ctcttcatat ttttgattcc atatttcagg tagttcagca
    36301 acatctaccc cttcattgaa aatcattttt tcgatttcat aacgaatgat gatatggaga
    36361 gggtaagtca aactgtctgc ctcgatacga accaaacttg attttgtatg gttcaatgaa
    36421 cgataaaatt tgtcaaaagg aatatcatcg aatgttcctt ctgcacattc ttgaaagaaa
    36481 ggatattgct ttttccagaa gctgcgacta cttccaataa tgatctcgtt aaacaacgat
    36541 tgtgattcat ggatacccat tgatgtacct tcataaaccg gtgtatacgc atatttatca
    36601 tcgatgtctt gttcatacat accatgacca gcctcgtgga tcactccaaa aacagccatt
    36661 ttgaaatctg tttcgttcca tcgtgtcgtg attcgtgcat cattcggatt aattccagtc
    36721 ataaatgggt ggacagtgtc atctaaacga ccacgactaa aatcataacc aagttgttcg
    36781 attactttca ctacaaatct cttctgttgt tttttcgtca ttctccgatt taaaaagtct
    36841 gtttcaggtt cagtcccttt ttcttggagc gtacggcgga tcgaaagaat tccttcttta
    36901 agctgatcaa ataccttatc aaggatttcg acagtcatcc cgggttcgta ttgattcagc
    36961 aaaacatcat aattcgtctt ttcatctttt ctccagtaag gtatcaactg ttttgttaat
    37021 tcaatatttt gattcaatgc gtgttgaaaa tcagaaaatt gttgtgtttc tctagctttt
    37081 tgccattgtc cgtgtgctga agaaacggca gctgtatatt ttctcataag atcttccggt
    37141 acgcgatgtt ccaaatcata ctcttctttt actttggcaa aaacggcagt tccaacttcc
    37201 gacaattctt ccggatgttc agaaaaatag cgaatggctt ctttgatttt tggaccgatt
    37261 ttttttgaaa aataaatgct gtaaagatag ctgtctactt cacttcggta tgcactagct
    37321 ttttcaggca tacctgtttg tgtatcccaa tcgaggattc ctaaagcttg ttgtagaaga
    37381 ttgatttctt ttacttcttg taaaaactct ttttctttca ttccctattc ctccaaacat
    37441 ccaattgaat gaatcaatta gtaagattat tcgtctttct ctttttttct aggcggacga
    37501 gttccccaat actggaatag atccgtacgc aatgcaccat tgtacaattt tctttttttg
    37561 gttgcttttt gtccgtaaaa ttcctcaaac gctaagtcgc tagtcaagat atatttactc
    37621 catgttttta atggacggaa tacatcgccc atttctttat ataatttccg aacagtttct
    37681 tcttctccta gacgttctcc gtaaggcgga ttagccacga tgaccccgta ttctttatcc
    37741 gttttgaagt ctttgactgc ttgttgctta aaggtaatcg aagagcctaa tccgatttct
    37801 tctgcattgg ctttggcaat ttcaatcatc cgtccattga tatctgaacc agtaatatct
    37861 aattcaatat cataatctgc tttttcttcg gctgctgatc gaacattttc catgatttct
    37921 tgactgaacc aatcccaaga ttcgcaagca aactcacgat tgaatcctgg tgcgatatta
    37981 tggcctatta aggctgcttc aatacaaagc gtacctgaac cacatacagg atcataaaat
    38041 ggacggtctt ttcgccaatt tgtcaacata ataagtgctg ctgccatgtt ttctttcaat
    38101 ggtgctccac ctttttctaa gcgataacca cgtttgaata aacttggtcc tgtcgtatcc
    38161 agagttacca tcacatggtc tttcagcaaa gctacttcca actgaaaatg tgcgccagtt
    38221 tccgtcagtg gtacagaagc tggacggtga taataggtac ggagacgttc aacgattgct
    38281 ttttttgtga tcgcttggca atcaggtgtg ctatatagtt tcgacttgat cgattttcca
    38341 gctacaggaa aagctgcatc tagtggaaga aaatcttccc aaggcaatgc ttttacgttt
    38401 tcaaacaatt catcaaacgt atacgcatcg aattccccaa ccacgatttt tactcgatct
    38461 gctgtacgta accataaatt agctgtagcg atcgtttcca ttgttccttc aaaacgtgca
    38521 cgtccgttct caacttggca ttcgattcca agatctctta attctttacc aaccagtgct
    38581 tctagcccgc ttgccgcagt tgcgacgagg ttgaacttcc tgtttttttc catacacaat
    38641 ctccttattt ataaaaaaag gaggtgggac atcagtccta ccccacactt ttactcaaat
    38701 tccgaataac cggtgataca aaagtttgtt ttgtaataag aacgattcat ctgaagtatt
    38761 gaaaaatagc tttcaaatgt ttttcttgtt tcttataacg cattactttt gtcacgacct
    38821 aattccctga tgtttgcggt tttctataag ccatgttctg tactttgctt ttccagggag
    38881 aaaaaacaaa gcggtaacca tctatctgca gttatactgc ctttttgtcc tgttcttctt
    38941 ccagacaaaa agcgccccta ccattgtttg ggttgctcgc tcgaggggtt taccgcgttc
    39001 caccggatgg gtttccccag ccgcttcgtc actgtggcac tttcaaggat acttaagcat
    39061 agccgaagcc ttagcttgtt ttcctgccgt tactccaaag agtaccttag cttatggttt
    39121 cgctaagcac gaacactaca ggcatcacag cctgtgcgag catggacttt cctcagcctg
    39181 atcaagtcag accgcgatta cccgaaaacc gcaaattcat taatcaaaaa ttaaaattga
    39241 cgtgtttttt cattatcatc taagcttgtg tctgcatttg tgtagttatt tggattaggt
    39301 tgtgctggtt tgactgcact tgcttgttga tcaagctttt taccaaatac ttcacgttca
    39361 agatttgaca agcgttttaa gatatcaaaa ttggtcactg ctgcgctctt tggtgcctct
    39421 gtttgttgca cgcgtgtttg agctgctcct tgagttttag ataattgagc tacttttgca
    39481 ctcaatcgat cgttttcttc ttgtaaagca aggagttctt tgctgtaagt ttcataatct
    39541 ttgatgatat tgtctaagaa ttcatcaact tcaacgggat catagccgcg catttttgtt
    39601 ttgaattctt gttgtaaaat gtctttagga ctataaacca aattcgccat atttacacct
    39661 ctagttttca tttacgtaat aacatttcta cctttttcat tgtataggaa aaaagttgat
    39721 aaatcaaaca caatatcgat tattcactaa aattttgaag atcgtccata ctgatcagcc
    39781 gaatttcgta gggatgatct gcttggtaat tctgtgcatc ttcaaaaaaa tacttcgttt
    39841 ttccagggaa ttcttcatca tagatcagca gacagcccac tgtatgctca agtataaaac
    39901 gagtgtgatt ctttagctga ctaggtgatt ggtagggttg atggctaaca gcttcgacat
    39961 aatctgctaa ttgttctgct aattgttttt ttcttggttc ccttcattcc agttattacc
    40021 aaaatcagaa aaaggataca aaataccaag agatagttcc ggatattctt ctttcaattc
    40081 cgctactatt tgcacagccc atagttcgac gcccaaattc ccactgacaa gtacccattc
    40141 cagcccttct tctgctaact gatggagttc tttttttaaa acatttttaa caacttggat
    40201 tttaggatca ttttctttaa aaacaccgag ttcgaaactt cgatacccag acacatagat
    40261 tacttttgac ttatccatct cagattcctt ttctttctat ccgctttttt gctataatgt
    40321 ggcctaggag tgtgattaaa gatggtattg cgctatccaa atggccaacc ttattataag
    40381 aatgcctctc ccgtcgaaaa aattcaagaa aaaagaaatc agatcactga attcggcaat
    40441 cgggggatgc gctttgaaga agcaatcaat gaaagtaata aatattatct ttcccgcggg
    40501 ttggcggtta tccataaaaa gcctacccct atccaaatcg tgaaagtaga ctatcctaag
    40561 agaagtgctg ctgtgatcaa ggaagcctac tttacccaag cctcaactac agactataat
    40621 ggcgtctatc gaggctttta cttggacttt gaagcaaaag aaaccaagaa caaaacatct
    40681 tttcccttta aaaacttcca tgcccatcaa atcgaacata tgaaagcatg cctcgaacaa
    40741 aatggggtct gctttgtctt gttatggttt tcgtctttga agcgctgttt ttatttgaat
    40801 agtgttcatt tgattgctta ctgggacgcc caaaaggaaa aaggacgcaa atcactacct
    40861 ttatctttga tcgaagaact aggaatagag atcaaaactg gtatttctcc gcggattcct
    40921 tatttggacg cggttgactt ctatttatca acattatcta ttgaaggaga aagcaagaat
    40981 ggcaaatgaa caaactagaa gctctagacg acagaaacag cctactccta aaaagagtgt
    41041 gaagaagaat tctggcaaag gttctggaaa gtcctctgga acacataaaa aaggattatt
    41101 tatcaagatc cttttaggaa tactctcatt tttctgtatc ctctttttag caggtgtagg
    41161 acttttctgg tattacgcaa aagatgcgcc cgaattgacg gataaaaaat tagacgctac
    41221 cgtatcttcc aaactatata cacaagatgg cgaactattc gaagatttag gcgcagaaaa
    41281 gcgtgaaaaa atttctgcga atgaattacc gaaaacatta gaagatgcga tcgtgtcggt
    41341 agaagatcgc cgtttttata aacacatcgg ggttgaccct attcggatca tcggttctgc
    41401 cctctctaac tttacctctg gcggactgca aggcggaagt actttgacac agcaattgat
    41461 caaactttcc ttcttctcca ccagtgccga ggatcagact ctgaaacgca aggcccaaga
    41521 agcgtggatg gctgtaagat tggaacaaaa aaaatcgaaa caagagatct tgacctacta
    41581 cgtaaataaa gtgtatatgt caaacggctt gtacgggatg gagacagctt ctgagatgta
    41641 ctttggtaag aaattatcag aactttcttt accgcaaaca gcattgcttg ctgggatgcc
    41701 tcaagcccct tccgcctatg atccttatgt ttatccagat caggcgaaga aaagacgaga
    41761 tactgttttg tatacgatgc ttcaaaacga aaaaatctct caaacagaat atgatcaagc
    41821 tgttaatgta ccggtaacgg atggtctgca agaactaact caatccgatg acaatactaa
    41881 gatcgtcgat aattatgtca aagaagtcat caatgaagta caagaaaaaa cagataagaa
    41941 tgtctacaca gacggtttag agatttatac gaatttggat ctggacgccc aaaagaaact
    42001 atatgatatc gtcaacacgg atcagtatgt ctcttaccca gacgacgaga tgcaagttgc
    42061 ctccaccctt atcgacacca ataccgggaa agtaaaagcc cagattggcg gacgtcacat
    42121 cgcagaagac gtaactttag gcaacaactt agcagtgaat acttcccgtg attttggttc
    42181 tacgatgaaa cctgtcacag attacggacc agcttttgaa tatttgaaat attctactgg
    42241 aaaaactatt actgacgcac cgtataacta tgaagggact tcgactcctg taggaaactg
    42301 ggataatcaa tatatgggca ccattacttt acgccaagca ctttacttat cccgtaatgt
    42361 tccagcagtc aaattattca atgaagtcgg ttcagataaa gtggcttctt tcttaaaaaa
    42421 tcttgggatc gaatacagta cgatccatca atcgaatgcg atctctagta atacggaaga
    42481 acaagatggg acaaaatacg gagcttcttc cttaaaaatg gcagcagcct atgcggcttt
    42541 cgccaatgga gggacttatt acaaacctca atatgttaat aaaatcgtct tccaagacgg
    42601 aacagaagaa acttatgagc cagatggaaa aacagccatg tctccagaaa cagcttatat
    42661 gatcacagac attttaaaag atacgatcac tgaaggaact ggtacgaatg cgcaaatcgc
    42721 tgggttatat caagccggta agaccggaac atccaattac acagatgacg agtatgcaaa
    42781 acttggtatt tcttctgggg tgtatccaga tatcctattt gcgggctata cgccaaatta
    42841 ttcgatttcc gtgtggacag gttacaataa aaaaatgaca cctgtcactt cagaatcgtc
    42901 acacgtagcc tctgatgttt atcgcgagct tatgcagtat gtttcagcta acgtgacaaa
    42961 tacagactgg gagatgccaa gcggattaat tcgtgtgggc ggtgaactgt actacaaaga
    43021 tcaatataca gctagatcga atgcaattac accatcaaca actattccat cttccagcta
    43081 tgtccagact cctggaagct caacaacaga gacaacgact caaagctcta gctctactag
    43141 tcaaagcgaa tcaactgctg agtcctcaaa agaaagcacg acagcagaaa caagcgagcc
    43201 agcgtcttca acaacggttc cttcttcttc atccgaagaa agctcgacac cgtcttcatc
    43261 tgctcctcct gcttcttcca gtgagccagc aactagcgga gcggatgctg caaatgacca
    43321 tacaccatcc agcagcacct cagcttctgg aaacagatag taaaaaagaa accacgtcta
    43381 caagcgtggt ttcttttttg tgtctatgta aaaaacagct acaaataaaa atcatgtacc
    43441 aagctgtatt acatgatttt ttataagact atccagcata tacatgtgtg atctaacatt
    43501 tagcttaaaa attagaaaat ccatttttct tttatcatta catatacttt atccatttac
    43561 ttgtttataa ttaaatcggc taaatacaca ctagtctttt atttagtgcc acttcttaat
    43621 aatcccattg ttattatcaa ataaaaaata caatatatgg ttttgaataa ttttttgaaa
    43681 gaaggcttca ttttgagtta tctgatagta tgcttaatta tcataatttg tctgcaccat
    43741 ctttttttat cgaaaacaat acctcgttat tccaagaata aaaaagctga aaaattttgt
    43801 ttattattaa ataaatttac tttaattgct cctatccttg catttattat tttttctgta
    43861 cttttaagca ctactcttaa aggaaaattt atggaaagaa gctctcatgc aatgattctc
    43921 acttttttat ggttgttgtt caccagaatc tatatttttc tgatgagcct gaaaccgccc
    43981 aaatccatta gtttatgttt agttataaat ggtatttttt tattatcact tatcattttt
    44041 attacacctt tagatagata cgtcacttat ttatataatc ctttggaata ctggacttat
    44101 tttataggta tattggaagg cattattttc tacatcgggt attttcccaa taaaaataac
    44161 aacttttatt tttatcgcaa taaattgtaa taagttacca tcctagctac tgagtatgta
    44221 catttacttt ttcatgcaaa aaagataaac gaaacagctt ttctccataa taacaagata
    44281 attcatacat ttatttgcaa ttaaagaaac cacgtctaca ggcgtggttt cttttttagc
    44341 gccttttctt ttagacgcat ccgatttttc ccatcttgac atattgatag caaaggacat
    44401 acctcgcact tgggatttct tgccgtgcaa tgatatctac caaaaaaaat cagtgtatga
    44461 tgtgtcttta cccataattc ttgtggtact ttccgcatca gtgtttcttc tacttccatg
    44521 accgtagcat ctagtttgca gatcctcagg cgttttgaga cacgttccac gtgggtatct
    44581 actgcgattg ctggtatccc gaatgcatcc cccaatacga catttgctgt ctttctgcct
    44641 acaccaggaa gagacatcaa ttcttctctt gacgttggca cttgcccatc gaaacgttca
    44701 atcagctgct gtgcacacgc tttgatgttt tttgctttat ttcgatacaa accaatcgtt
    44761 tttattttaa gaatgatttc atcgattgaa gcttctgcta acgcatcggg tgtcggaaaa
    44821 gaggcaaaca aatctggcgt tgctttattc actgatacat ctgtcgcctg ggcacttaag
    44881 atcactgcaa tcaataactc aaatggatta ttgtgcttga gttctccatg tgcttcggga
    44941 aacattccat acatcgtttc tagagcttcc attgtttttt gtttgcttaa catattccgc
    45001 tactcctcca accaattttt cagtgagact ttcggcaatt cttcttgttg agctgactgt
    45061 tctacttctc tttgaagaag ctgctgtttt cttcgttttt ggtcttcttc cacttgttgt
    45121 tttgtacgta gattttttct ttcccacgat agcaaaatgc ggtcaacgta attaaaacta
    45181 taagcctgat ttaatacggc ttcccgcagt gctagcttca aaatttccgg ctgataatgg
    45241 tcttcttcta gccattgtcc gatccgctgg aactcgatcg aagaaagcgg gcgtccaaat
    45301 tcttgttcaa ataattgata cactgactgg atttcttttt cttgtgtttt ttcttcgtat
    45361 tttttttctt gtgtttttaa atactcacct aatagatcat agatggggta aagattatat
    45421 cgatctgctt ttttaccgtt gacaatcgtc gtttctatct tgataaatcc atttgttacg
    45481 agacgatcca gtatttgaaa aatctgatct tgtttgattc ccatatccat agcaatcatc
    45541 tgcaaatcag ggaaagtatc tccttcaagc tgtgccatat gcaactgtag aacaaataaa
    45601 aattcttcag attgtagacc gatcctatga taatttttaa gtaaaagatt agatatgact
    45661 gtttgtccag cgcttaagta attttctaaa ttcaacatga tttctcctcc gctcaatcac
    45721 agtatacgta aaaaaaacat gggaagcaag aacaggtttc ttttctattt acttcactga
    45781 tcggtcagat ttacaatgtc gtctacttga tttttgtttg ttcatgtttt tgatgcgtat
    45841 cttcactttt cggaataaat aaattaagta gatttcgctg ttccttagaa tgttgacaag
    45901 gaaacaaaat tactatacac tatttttgtt tccaagttaa cattattgca taaaggagtt
    45961 tttacatgta cagtttcaca aataaacatt ctaaagtttt tggatacgtc tctgtcttat
    46021 tcactggatt aggatttact atcgtcagtc ctgtacttcc gtttttaacg ctgccttatt
    46081 ctcactccat acatcagcaa gctttctata tcactttgct catgtctgtt tatgctctag
    46141 ctgccttctt atctgccccc attctcggta gcttaagtga tcattttggg cgtcatccga
    46201 tccttattat cagtttatta ggatcaagta tcggatattt gctttttggt ttaggaaatt
    46261 cgatatggat gttgttcctt ggtcgtatca ttgaaggatt gacggcagga gaaatctcca
    46321 ccttatatgc ttactttgcc gacatcacgg aacctaatga aagaaccaaa gtttttggat
    46381 ggatgggtgc tttagtcggt ataggaacaa cgctgggtcc tattataggg ggtctattag
    46441 ctgagttggg gaattctgtt cctatattta ttggagcact ctttactttc ttaaatgccg
    46501 tttatggcta cacatttatg ctagaaagcc ttccaataaa aaaacgttca gtagatttaa
    46561 gtttctccca tgtaagacct tttcaccaat tacaacaatt attcaagatt tcatccgtta
    46621 ttcctttatt gacagcaggt tttgctgttt ggttggcagc tggctctctt caatctatct
    46681 tttctcaatt ttcgattgac gcctttcagt ggaaggctgg gttggttggt ttaagcttct
    46741 ctttgattgg aatcttggat actgtctcgc aattgttcat tatgcctcga ttgctcaaaa
    46801 aattttcaga acagcagatc acaagaatag ggatgttcag tgaaatcctc gcctatctat
    46861 tcatcacatt atctggaatt cttctactgc cgattttgtt tttatttggg attatctgtt
    46921 atggtttcgg tgattctatt tttactcctg tattcaatgg ccaactatcc aattctgtct
    46981 cagataacca gcaaggtctt gtaatgggcg gtacccaaag tatacaatct ctatcgcgcg
    47041 tcatcggccc gctaatcgca ggacagcttt atgctgcagc accatttctt ccgacggtat
    47101 tcggattttt gcttatgatt tttgcctatt atcactacaa taaaaaatag tagcttcatt
    47161 tgctacttcg tataaattct ctgtatcgac cgataaaacg aaatactgtg tcaaattctt
    47221 cttttgacat attttcaaat acagtagcat ctcgctgtac ccatttttga tggagttgct
    47281 cgtgtctatt ccttattttt tgtccgattg gcgtcaattg aaaatagatt tcttttttat
    47341 tctcttcgct ttgataagtg tttatgatcc ctctagctat cagttttttt gttagcttac
    47401 tgatggctcc tctagtcatg taactagcat tcgctaattt tgtcacgtta ggatcaggga
    47461 ttttcccgat atgttcaatc acttctagtt catttagagt aaatcctgca aattcttttt
    47521 ccatttctgg tttattacga tgaataatct cattagctaa atccagatat tcttctatta
    47581 gcagttgtct tttatccatt tctacacctt ctttttcttt cattatattg tttttttgtt
    47641 tcctattcaa caaagataaa aatattttgt aggaaaaccc aatctgaaac caag
//
LOCUS       DANKEF010000019        43405 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000019 DANKEF010000000
VERSION     DANKEF010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43405
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.019"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..210)
                     /locus_tag="PWZ48_001186"
     CDS             complement(<1..210)
                     /locus_tag="PWZ48_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2260855.1"
                     /translation="MDTITPTNFKENIGKVLQEVVHSNSEIEITRDSKNGLNDGVIMI
                     SKRKWELLQEELYLQRTGTWAYVSDL"
     gene            complement(309..623)
                     /locus_tag="PWZ48_001187"
     CDS             complement(309..623)
                     /locus_tag="PWZ48_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260856.1"
                     /translation="MAEENKVVPFETLVINKEEFEQLFLDFFIQYDMIYDFSSSLDLF
                     ELEAEIIENSREKNGKIIISVEKFNELFSQTFEIDPDYYQPQEVFREFRNKIIGFSQT
                     TK"
     gene            complement(691..1185)
                     /locus_tag="PWZ48_001188"
     CDS             complement(691..1185)
                     /locus_tag="PWZ48_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260857.1"
                     /translation="MFKVYFPFQWLVKGILLVLLGLILFGSYLTIKARHFPEEKPIKI
                     VTKDLKIGETYQADGVELTFKNIEVTEKRNEFAQHDRVLQVTYTLINDTENPIRPQQW
                     SIENKDGEELYEYDMILDNPEYIESGKEETVNYYYELPIERQDYQIVVRRENGYIVGL
                     PFTL"
     gene            complement(1205..1492)
                     /locus_tag="PWZ48_001189"
     CDS             complement(1205..1492)
                     /locus_tag="PWZ48_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260858.1"
                     /translation="MNYPYFKVSASEETKEIFNNFYNQNKGVFGSKANMFRVMVSNLP
                     VLASPSNNKFNDPESIKFEQKISELESMISNEVIEKLDDIDQKLSYSLKNK"
     gene            complement(1708..3099)
                     /locus_tag="PWZ48_001190"
     CDS             complement(1708..3099)
                     /locus_tag="PWZ48_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2260859.1"
                     /translation="MGATITSKGRKKNLSSLLQYAANPSKKEKCVAVSGINCSDDSEE
                     ALQEMNLVRTKMNKSKDIAQAFHVIHSFDKSTSKELSIDKMHEIAVEFAEKAFPNSQI
                     IVASHNDKDHFHSHLVINNINMETCKRLRIDPKDLELMRKINDQVLNEHGLESVNNEY
                     YENLEKRTNLYSNDPSKMKYGKINYQEFVQNAIEATLEKCEVNSFEDFSDHLAKEYNI
                     EVYQFSKTNNKLGYVLYKQGVNFYERRGELITLGRDKQSRKEKSQYVERTFSARKLGK
                     RYSFESVSEQLEKNLEESHIKNFFNMEREQVMSLISGVNYKTGELNVPFVELDSIAQK
                     QFSDREGDEKFIKYDDFLREVDKHFVNTMSLEDDQEYTIKLHTDVNPQTLRSAKSKTI
                     SIKKTDQKELFLPAKGFENCSHTPSAKQIHYQISRIMNDDVKMYRAKKDYEFTQQMIE
                     NQQKRQQNFEMEF"
     gene            complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="PWZ48_001191"
     CDS             complement(3084..3695)
                     /gene="mobC"
                     /locus_tag="PWZ48_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2260860.1"
                     /translation="MDDKQKMYEKNRKHSEALNFRVDKKTYLELTLIAKQRGISRADV
                     CRERLKGLKYEPPLLAEDDRVVLTKQLNQIGNNLNQAVKALNIIAKYFREESEDKKEK
                     SLHFSFDASRYNIFKDELANSIAKKLMISYPDDIFLESNRSAPSFFKLSEVEKEYFEY
                     WIEKQTRPGTKEEYSQKVDRNLIHSIQIIASLKDEVKQLWELL"
     gene            complement(3699..3896)
                     /locus_tag="PWZ48_001192"
     CDS             complement(3699..3896)
                     /locus_tag="PWZ48_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260861.1"
                     /translation="MQLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKNEIESNFKCCSLEKNVRG"
     gene            complement(3887..6565)
                     /locus_tag="PWZ48_001193"
     CDS             complement(3887..6565)
                     /locus_tag="PWZ48_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260862.1"
                     /translation="MKIIEKKIANKKINWLVVNFLVLIAMIIFTSNIAHASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSAVNTIVIGLMILSLMFVGYKMITGKGTVDLKSVGMNVVMSIALI
                     LLMPTMISSGIQFAKTFYKDSTTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNNLTRKNFQQTDLTQVLTDKVIDKLEKENPIADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNSGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSNGFNRAKGSGE
                     GEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERATKPFKSMRDI
                     ANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSVPTRLSDRKDGAKVEN
                     ADRIMSSSERMREAMKNNAESNNNPASAIQQKVQQDIEERKNAMHGQAKSAEELINQK
                     RHEAKYTPEAMNREEIMLRKRVEGRTGANIDGKEALTKERIQEAKNSNTGLEKLVKEN
                     PQNSTSSHGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNAQGTKTIDVRENLQSS
                     STSQPKTVDVRENVQNSTSSQGFKSVDVRENVQGSSASQPKTVDVRENVQSSTSSQGF
                     KTVDVRENIQKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKSR
                     RRFTYEDNELFRDTLNDPNPLFDNLLRNKRGGLSCN"
     gene            complement(6552..6770)
                     /locus_tag="PWZ48_001194"
     CDS             complement(6552..6770)
                     /locus_tag="PWZ48_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260863.1"
                     /translation="MIKNRLIDQVWCLDQNTDSLELIETICFNTIVLDLRAENDNCVT
                     HVIINQKMAQKLSESLRKWAKGGNDENN"
     gene            complement(6846..7898)
                     /locus_tag="PWZ48_001195"
     CDS             complement(6846..7898)
                     /locus_tag="PWZ48_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2260864.1"
                     /translation="MNEKEVESLVEKQGYSNSDIYAELYYQYPIFLNVNEKYKKMKLS
                     SKLVYMLLKDRNRLSLKNNLYDENQKIFLIFEIKNLVEISGMSKNTILSALDELQKVK
                     LLVKRKNGFNKSKMQMFPNFYYLLKPVLCKDDVYKINKITHQENRQKNLIVQNLNYGT
                     NQYSVDNSKFELCDTSMKTRDKNNFADSSKNELELYKSYKDSKDIKESHNDDLLESLS
                     TKNNKLDEELIETFIEENLLKQIWGESIVNCLKLASNSDYRQFDNWYKKFKYAIRSAE
                     KEQKIQLKIIYEICNNKYFVDHVREQLSMTLRDLIRRAKTDHRIKQKDDYIFASLKNK
                     ALELIELQISEGIDKQ"
     gene            complement(8051..8377)
                     /locus_tag="PWZ48_001196"
     CDS             complement(8051..8377)
                     /locus_tag="PWZ48_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260865.1"
                     /translation="MASWLIEEIENERRKIMDAGITVMLDKQQTNQLKNYVFEMTKEA
                     IDQARIDTGLERPFLKGKEMAKYLNVSYTTFLKFKRMGLPVILLEKMELFSKEECKKW
                     ILSHQI"
     gene            complement(8389..8589)
                     /locus_tag="PWZ48_001197"
     CDS             complement(8389..8589)
                     /locus_tag="PWZ48_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260866.1"
                     /translation="MITLNDQFIRSLRRHRADLILTKNDAAKLIGINRKTYVKIENGS
                     KESIRASTYQKLVNWLLNDLKI"
     gene            8712..9314
                     /locus_tag="PWZ48_001198"
     CDS             8712..9314
                     /locus_tag="PWZ48_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2260867.1"
                     /translation="MEFTIGGVTLNRIKFYIDSSKKTIKQISTETGINYTTLTNYYRG
                     ERNPRNTETWNILADYFHVPVSYLMGLDETNSIEQPNLDRIKTARLEKKDKIVKNIAS
                     LDSDYFTELSNLYFSMTSLLEVKLQEDDSDFLFLKSILEDSFEYTILFAKQKKFINKA
                     LNLNIDERKELESELFGNLTQVQVNNLKREITARLISKLQ"
     gene            9369..10535
                     /locus_tag="PWZ48_001199"
     CDS             9369..10535
                     /locus_tag="PWZ48_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2260868.1"
                     /translation="MSIQKYTTKSGARYSVRLYVRKDENGKQEYYVKKGFKTEKEAKL
                     HEARKKVEIEDTGYHKPSNDLFITVYEEWLPSYRNRVQETTYQRTKDLFRLHILPKFG
                     NKSISKITPVHCQNAVNEWALKFKNIKQLKSYSSQIFEYAIFAELLHRNPMANTNLPK
                     REKKENENYFSLEELKAFWDILNKEEPLKHILIFQLLITTGIRKGELAALHWSDIYFE
                     EQLMYVRKSYATIRNEEKNARRKTVRIQKNTKNISSERILPIDAQTIEMLKIWKKEQA
                     RELLQFGINTNTKNQLIFTYVNADGDINQPLHADYSNNILKRLEKKYKFKHVTIHGFR
                     HTHATLLLEGGASIKETQDRLGHKNAETTLNTYSHVTEKAQRNALEKFIAYTGL"
     gene            complement(10624..11016)
                     /gene="rpsI"
                     /locus_tag="PWZ48_001200"
     CDS             complement(10624..11016)
                     /gene="rpsI"
                     /locus_tag="PWZ48_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2260869.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(11032..11475)
                     /gene="rplM"
                     /locus_tag="PWZ48_001201"
     CDS             complement(11032..11475)
                     /gene="rplM"
                     /locus_tag="PWZ48_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDL2260870.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(11994..12461)
                     /locus_tag="PWZ48_001202"
     CDS             complement(11994..12461)
                     /locus_tag="PWZ48_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2260871.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(12581..14119)
                     /locus_tag="PWZ48_001203"
     CDS             complement(12581..14119)
                     /locus_tag="PWZ48_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDL2260872.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            14199..14885
                     /locus_tag="PWZ48_001204"
     CDS             14199..14885
                     /locus_tag="PWZ48_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDL2260873.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(15086..15613)
                     /gene="msrA"
                     /locus_tag="PWZ48_001205"
     CDS             complement(15086..15613)
                     /gene="msrA"
                     /locus_tag="PWZ48_001205"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDL2260874.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(15697..16374)
                     /gene="pgmB"
                     /locus_tag="PWZ48_001206"
     CDS             complement(15697..16374)
                     /gene="pgmB"
                     /locus_tag="PWZ48_001206"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDL2260875.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(16371..17501)
                     /locus_tag="PWZ48_001207"
     CDS             complement(16371..17501)
                     /locus_tag="PWZ48_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2260876.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(17501..19684)
                     /locus_tag="PWZ48_001208"
     CDS             complement(17501..19684)
                     /locus_tag="PWZ48_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HDL2260877.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(19681..20706)
                     /locus_tag="PWZ48_001209"
     CDS             complement(19681..20706)
                     /locus_tag="PWZ48_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2260878.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(20751..21578)
                     /locus_tag="PWZ48_001210"
     CDS             complement(20751..21578)
                     /locus_tag="PWZ48_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2260879.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(21575..22642)
                     /locus_tag="PWZ48_001211"
     CDS             complement(21575..22642)
                     /locus_tag="PWZ48_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDL2260880.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(22668..23504)
                     /locus_tag="PWZ48_001212"
     CDS             complement(22668..23504)
                     /locus_tag="PWZ48_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2260881.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(23501..24370)
                     /locus_tag="PWZ48_001213"
     CDS             complement(23501..24370)
                     /locus_tag="PWZ48_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2260882.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     SVVLLSRKKVDLK"
     gene            complement(24440..25723)
                     /locus_tag="PWZ48_001214"
     CDS             complement(24440..25723)
                     /locus_tag="PWZ48_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2260883.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(25737..27422)
                     /locus_tag="PWZ48_001215"
     CDS             complement(25737..27422)
                     /locus_tag="PWZ48_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HDL2260884.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            27697..28722
                     /locus_tag="PWZ48_001216"
     CDS             27697..28722
                     /locus_tag="PWZ48_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260885.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(29317..32991)
                     /gene="rpoC"
                     /locus_tag="PWZ48_001217"
     CDS             complement(29317..32991)
                     /gene="rpoC"
                     /locus_tag="PWZ48_001217"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDL2260886.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(33049..36666)
                     /gene="rpoB"
                     /locus_tag="PWZ48_001218"
     CDS             complement(33049..36666)
                     /gene="rpoB"
                     /locus_tag="PWZ48_001218"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2260887.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            37394..38362
                     /locus_tag="PWZ48_001219"
     CDS             37394..38362
                     /locus_tag="PWZ48_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2260888.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            38380..39285
                     /locus_tag="PWZ48_001220"
     CDS             38380..39285
                     /locus_tag="PWZ48_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2260889.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            39282..40088
                     /locus_tag="PWZ48_001221"
     CDS             39282..40088
                     /locus_tag="PWZ48_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2260890.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            40224..41213
                     /locus_tag="PWZ48_001222"
     CDS             40224..41213
                     /locus_tag="PWZ48_001222"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HDL2260891.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(41530..42054)
                     /locus_tag="PWZ48_001223"
     CDS             complement(41530..42054)
                     /locus_tag="PWZ48_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HDL2260892.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            42341..>43405
                     /gene="scm"
                     /locus_tag="PWZ48_001224"
     CDS             42341..>43405
                     /gene="scm"
                     /locus_tag="PWZ48_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2260893.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSS"
BASE COUNT        12445 a         9024 c         7631 g        14305 t
ORIGIN      
        1 taaatcagat acataagccc atgtacccgt tcgttgtaaa taaagttctt cttgtaacaa
       61 ctcccatttt ctcttagaaa tcataatgac gccatcgttc aatccatttt tagaatccct
      121 agtaatttct atttcagaat ttgaatgaac tacttcttgc aacactttac ctatattttc
      181 tttgaagttt gttggagtaa tagtatccaa aatgaatacc tctcttctct atacctctat
      241 tatgaagtgc ccataaaata aagcaagcgt tgaggaataa ttctatcaac acttgcttta
      301 ttaagctttc attttgtagt ttgactaaaa ccaataatct tatttctaaa ctctcgaaaa
      361 acttcttgtg gttgataata gtctggatct atttcaaatg tttgtgagaa taattcattg
      421 aatttttcta ctgaaataat gattttacca ttcttctctc gactgttttc tattatttct
      481 gcttctaatt caaataaatc tagtgacgaa ctaaaatcat aaatcatgtc atattgaata
      541 aaaaaatcaa gaaaaagttg ctcaaactct tccttattta tcactaatgt ttcaaacgga
      601 acaactttat tttcctcagc cattctccca gttccatttt caaaaatcac aaaactgata
      661 ttaccttaaa aactactaac aaaataaaac ttataaagta aatggcaacc caactatgta
      721 accattctct ctacgtacta cgatttggta gtcctgcctt tcaatcggta attcataata
      781 gtaatttact gtttcttctt tgccactttc aatatactct ggattatcca atatcatatc
      841 atactcataa agttcttcac catccttatt ttcaatcgac cattgttgtg gacgtatcgg
      901 attttctgta tcatttataa gtgtataggt cacttgtaat acacgatcat gttgtgcaaa
      961 ttcatttctt ttttcggtca cttcaatatt tttaaatgtt agttcaaccc catctgcctg
     1021 ataagtttca cctattttta aatcttttgt tacaattttt attggctttt cttctggaaa
     1081 atgtctggct ttaattgtta gataacttcc aaaaagaatc aatcctagta gcactaaaag
     1141 aattccttta acgagccact gaaacgggaa gtaaacttta aacatctttt ttctcctccg
     1201 ttttttattt gttttttaga gaatacgata acttttgatc aatgtcatcc agcttttcaa
     1261 ttacttcatt acttatcatt gattctagtt ctgaaatttt ttgttcaaat ttaatgcttt
     1321 caggatcatt aaatttatta tttgatggac tagctaacac tggtaaattc gaaaccataa
     1381 cacgaaacat atttgccttt gaaccaaaaa ctcctttgtt ttggttgtaa aaattgttga
     1441 atatttcttt agtttcttct gaagccgaga ctttaaaata tggatagttc atttttttct
     1501 tccacctttc tttttttggg agggagtctg tagggagctt gctccctcag agatttaccc
     1561 accatctgac ttgccagaaa gtagcctagc gtgagcgggg cggattgact ggcaagtaga
     1621 tgggtagctg gcgtatgtcc taaaaactgc ttctttccaa gcaactcttc ataggcaaaa
     1681 aactggaaag tccactaaca agcccattca gaattccatt tcaaaatttt gttgcctttt
     1741 ttgctgattt tcaatcattt gttgtgtgaa ttcataatcc tttttagcac gatacatttt
     1801 tacatcgtca ttcattatgc gactgatttg atagtgaatc tgtttagcag aaggtgtatg
     1861 agaacaattt tcaaaaccct ttgccggtag aaataactct ttctgatcag tctttttaat
     1921 agaaattgtt ttacttttcg cactacgtaa agtttgagga ttcacatccg tatgaagttt
     1981 aatcgtgtat tcttgatcat cttctaaaga cattgtattt acaaaatgct tgtctacctc
     2041 tcgcaagaag tcatcatatt taatgaattt ttcatcgcct tcacggtcgc taaattgctt
     2101 ttgtgcgatg gaatctaatt ccacaaacgg tacatttaac tcaccagttt tgtaatttac
     2161 tccactaatt agcgacataa cttgctctct ttccatatta aaaaaatttt ttatatgtga
     2221 ttcttctaaa ttcttttcaa gttgttcaga aacagattca aatgaatatc ttttccctaa
     2281 ctttcttgca ctaaaagtac gttcgacata ctgacttttt tcttttctag attgtttatc
     2341 tcttcctaaa gtaattaact cacctctacg ctcatagaaa tttacacctt gtttgtaaag
     2401 aacatagcca agtttgttat ttgtttttga aaattgataa acttcaatgt tatattcttt
     2461 tgccaaatga tccgaaaaat cttcaaaaga attcacttcg catttttcca acgttgcttc
     2521 gatcgcattt tgcacaaact cttgataatt aatttttcca tatttcatct tagaaggatc
     2581 gtttgaataa agattggttc tcttttctaa gttttcatag tactcattat tcactgattc
     2641 aagaccatgt tcattgagga cttgatcgtt aatttttcgc attagttcta aatctttagg
     2701 gtcaatacgc aaccttttac atgtttccat gttaatatta ttaatgacaa gatgactatg
     2761 aaaatgatct ttatcattgt gacttgcaac aatgatttgc gaatttggaa aagctttctc
     2821 tgcaaattcg acagctattt cgtgcatttt atcaatactt aactctttag aagtactctt
     2881 atcaaacgaa tgaataacat gaaacgcttg tgctatatct ttagatttat tcatcttggt
     2941 acgtactaaa ttcatttctt gcagtgcttc ctcactatca tcactacaat ttattcctga
     3001 gactgcaaca catttttctt tttttgaagg attagctgca tattgtaata gcgaggataa
     3061 atttttcttg cgtcctttcg atgttatagt agctcccaaa gttgtttcac ctcatctttt
     3121 aaacttgcaa taatctgaat tgagtgaatt aaattccgat caactttttg ggaatactct
     3181 tctttcgtac ccggtctagt ttgcttttct atccaatatt cgaaatattc tttttctact
     3241 tctgataatt taaaaaaaga gggggcactc cgattggact ccaaaaaaat gtcatctgga
     3301 taactaatca tcaacttttt tgctatgcta ttcgcaagtt catctttaaa aatgttgtaa
     3361 cggctagcat caaaactaaa atgtaggctc ttttcttttt tatcttcaga ttcttctcta
     3421 aaatatttag caatgatatt taatgccttt accgcttgat tcaaattgtt accaatttga
     3481 tttaactgtt tggttaaaac taccctatca tcttcagcta acaacggcgg ttcatatttg
     3541 agtcccttta accgctcacg gcaaacgtct gctctagaaa ttcccctttg ttttgcaatc
     3601 aatgtcagtt caagataagt ctttttatcg acacgaaaat ttaaggcctc actatgtttg
     3661 cgattttttt cgtacatttt ttgtttatca tccattgatt atcccctcac atttttttct
     3721 agagagcaac atttgaaatt gctctctatt tcatttttca tctttaattc ttcttgaatc
     3781 atcagttcaa tataattagt gaccgtgatt ttttcatttg ttgcgttctt tactgattca
     3841 cataattcgt caagttctaa gaaatcatgc gtactaatct ttagaattaa ttgcatgaca
     3901 atccccccct tttatttctt aataaattat cgaaaagtgg attaggatca ttaagtgtat
     3961 ctctgaataa ctcattgtct tcataagtga atcttcgtct tgattttgat cccttatcat
     4021 tttcacggat ttcactatca ataatcactg tttcatgatt tgaatcaaat tttttcgctg
     4081 actttctgtc ttcaacgata ttgatttttt gtgttttttc tttcatttct ccactatctt
     4141 tttgaatgtt ctcccgaaca tctactgttt tgaatccttg actacttgta gaactttgta
     4201 cattttctcg gacatctact gtttttggtt gacttgcact gctaccttgc acattctctc
     4261 ggacatccac tgatttaaac ccttgactac ttgtagaatt ttgtacattt tctcgaacat
     4321 ctactgtttt tggttgactt gtactgctac tttgcagatt ttctcgaaca tcaattgtct
     4381 tagtaccttg agcatttgaa gaactttgta aattctctct tacatcaaca ttccgtccaa
     4441 catgaccatt cagtgaacct tgaatatttt ctcggacatc tactgatcgg ccaccatgac
     4501 tacttgtaga attttgtgga ttttctttga ctaatttttc taatcctgta tttgaatttt
     4561 tagcttcttg aattctttct ttggtcaacg cttcttttcc gtcaatatta gcgcctgtcc
     4621 gcccttctac acgtttcctt aacattattt cctcgcgatt cattgcttca ggcgtgtatt
     4681 tcgcttcatg acgcttctga ttaattaatt cttcagcaga cttagcttga ccgtgcatag
     4741 catttttacg ttcttcaata tcttgttgga ctttttgctg aattgcgctt gcaggattat
     4801 tattcgattc agcattattt ttcattgctt cacgcatacg ttcagaacta gacataattc
     4861 tatcggcatt ttcaacttta gcaccatctt ttctatctga aagacgtgtt ggcacggaat
     4921 cagcatattt tccagtttca tcttctttat ttttagctaa cataggtttg ctatttttat
     4981 taatagcgga cactgtacca tcatctaaac cttctttaaa cttattcttg gtatcattag
     5041 ctatatcacg catactctta aatggttttg ttgctctttc actagccatt gtagctccat
     5101 ctgaagctaa accggataat ccacgttcat gggcatatcc caaagctctt ccagttttgc
     5161 cagctttttt tgatagagtt tcgccgaaat tcttttctgg ttttcgatct tctccttctc
     5221 cgcttccttt agcacgatta aatccatttg atccttttct aaacagcatt gcgcccattc
     5281 caaaagcaga agcaagttgc ccatagcctt ctttgagtcc aatatcaaca ccaaagtatc
     5341 tcatgactgt tccactgcct ttgattaaaa caaaaacggc acagatataa gcaattgttt
     5401 ttataaaaat atttgttgag ataccttgtc ctgagttcag aaaattgata aacatagcaa
     5461 acattgataa cccaaatcct tgaaacccaa cagttaagta acattgtaga atatctgata
     5521 gaacaacttt tgaacgctgt cctgtctcaa tatctgttgc aaaaacaaga acacctaaca
     5581 cacgtttaaa tgcaagttct agtattgctg taatgatcac aaacgcgctg aacacatagg
     5641 cgattgctaa cgctgtcaac ccaattgaaa tcccccatag atttgcttgg tatcggtaat
     5701 agcctgattt caagctatca gaaaatgttg ataagaagtt gtcagaaaac tttgtagcaa
     5761 caaattcatt attagaattt tcaactaatt cataacgaag attgtccgca attggatttt
     5821 ctttttccaa tttatcaatc actttatccg ttaacacttg tgtgagatcc gtctgttgaa
     5881 aattttttcg tgttaaatta tttcgatcct ccgttttatc aatgcttgag tactgatctg
     5941 ttttattaat atatgctaaa tctgttactc cttgttttat caatgaccaa gcgactccgt
     6001 catctgaatt tgtaatcgta gtagagtcct tataaaatgt tttagcaaat tgaataccac
     6061 tagaaatcat ggttggcatt aaaagtatta atgctataga catcactacg ttcattccaa
     6121 ccgattttaa atccactgtt ccttttccag taatcatttt gtaaccaaca aacatcaatg
     6181 aaaggatcat taatccaatg acaattgtat ttacggctga ttgataaaca ttatttagcc
     6241 ctgtactttc aatgaaattg aacaaatcca gcacatctgg aatcatattt tgaatcgaat
     6301 ctgtcacagt aaacatgcct tttaccacag cccatccgag ccaaatcccg aactgactaa
     6361 ggaaatctcc attgtacttt tttaggtaag gtctataaag agaattattc aaaaactctg
     6421 tttgactttt tgtgtcatca aaccaattga aaatactcgc atgagcaata ttacttgtaa
     6481 aaataatcat agctatcaga acaagaaaat taacaaccaa ccaatttatt tttttattgg
     6541 caatcttttt ttcaattatt ttcatcgtta cctcccttag cccattttct gaggctctca
     6601 gagagcttct gagccatttt ttgattaatt atgacatgag ttacacaatt atcattttca
     6661 gctcttaaat cgagaactat agtgttgaag caaatagtct cgattaattc gagactatct
     6721 gtattttgat ctagacacca aacttgatct attaaacgat ttttaatcaa aataacccct
     6781 cctatattca aagttctcaa atgaagtgga gatacccctc catttcattt gactcaattc
     6841 tattcttatt gcttatctat tccttcgctg atttgcaatt caattagttc taacgcttta
     6901 ttttttagag aagcaaagat ataatcgtct ttttgtttga tacgatgatc tgtttttgct
     6961 cttcgtatca agtcacgtaa agtcattgat agctgttctc taacatgatc tacaaaatac
     7021 ttgttattac aaatttcata gataattttc agttgaattt tttgttcttt ttcagcagat
     7081 ctaatcgcat atttgaattt tttgtaccag ttgtcgaatt gacgataatc actattgcta
     7141 gccagtttta agcaattcac aatactttct ccccatattt gtttaagaag attttcttca
     7201 ataaatgttt cgatcaattc ttcatctaat ttattatttt tagttgaaag tgattcaaga
     7261 agatcatcat tatgtgattc tttaatatct ttactatctt tataagactt atataattct
     7321 agttcatttt ttgaactatc agcaaaattg tttttgtctc ttgttttcat tgatgtgtcg
     7381 catagttcaa attttgaatt atcaacagaa tattgatttg ttccatagtt caaattttga
     7441 actatcaagt ttttttgtct attttcttga tgggttatct tattaatttt ataaacatca
     7501 tctttgcaca gaacaggctt taacaaataa taaaaatttg ggaacatttg cattttactt
     7561 ttattgaagc cattttttct tttcacaaga agtttaactt tttgaagttc atctagtgct
     7621 gataagattg tattttttga cattcccgaa atttctacga gatttttgat ttcaaatatc
     7681 aaaaaaattt tttgattctc atcatacaag ttatttttca atgaaagtct atttctatct
     7741 tttagtaaca tataaactaa cttagaactt agtttcattt tcttatattt ttcatttacg
     7801 tttaaaaaaa taggatattg atagtataat tctgcatata tatcagaatt agaatagcct
     7861 tgcttttcaa ctaagctttc aacttctttt tcattcattg tcacacctgc ttctgatttt
     7921 atttgctata atagattcaa ataaagtttt ctcagaactt tattattgag tgttcccccg
     7981 atctgcaaac tgacgggaac actcttttta attttttcta cttcatcaaa tttaaaaaac
     8041 gaggtgtttt ttaaatttga tgactcaata tccatttttt gcattcttct tttgaaaaca
     8101 actccatttt ttctaataaa ataactggca atcccattct tttaaatttc aaaaaagttg
     8161 tatatgatac attcaaatat ttagccatct cttttccttt tagaaatggt ctttctaatc
     8221 cggtatcaat tcttgcttga tcaatagcct cttttgtcat ttcaaaaaca taatttttca
     8281 attgattcgt ttgttgttta tctaacatta ctgtaattcc tgcatccatt atttttctcc
     8341 tctcattttc tatttcttca atcagccaac tcgccaagtt gtatttattt agattttcaa
     8401 atcattaagt aaccaattta ccaacttttg ataagtagat gctctaatac tttctttgct
     8461 cccattttcg attttgacat atgttttccg atttatacct atcaattttg ctgcatcgtt
     8521 tttagtaagt ataagatcgg ctctatgtct tcttaagctt ctaatgaatt gatcattaag
     8581 agttatcatt cagcgcctcc tttcaaatta ttcactttgt ggtttttatc ttaataagca
     8641 cattttaaac cacaaaacga gtaatatcaa ttaaaatatt actcaatttg tggttttcat
     8701 gctataatag tttggaattt actatagggg gcgttacttt gaatagaata aaattttata
     8761 ttgattccag taaaaagacc attaaacaaa ttagtacaga aactggtatt aactatacaa
     8821 ctcttacgaa ctattaccgt ggtgagcgaa atcctagaaa tactgaaaca tggaatatcc
     8881 ttgctgatta tttccatgtt cctgtaagtt atttgatggg attagacgaa acaaattcca
     8941 tagaacaacc taatcttgat agaataaaaa ctgctcgttt agaaaaaaag gacaaaatag
     9001 tcaagaatat tgccagtctt gactcagatt attttactga attatctaat ctatactttt
     9061 ccatgacttc tttacttgaa gtcaaacttc aagaagatga ctccgacttt ctttttttaa
     9121 aaagtattct agaggactcc tttgaataca cgattctatt tgccaaacaa aagaagttta
     9181 ttaataaagc tttaaactta aatatcgatg aaagaaaaga gttagaatct gagttatttg
     9241 gcaacttaac ccaagtacag gttaacaatc taaaaagaga gataacagct cgattaatat
     9301 caaaacttca ataaaactaa ttattcaatc agccaactcg ccaagttatc agattggaga
     9361 gattagttat gtcaatacaa aagtacacaa caaaaagcgg tgcacgatat agtgtccgct
     9421 tgtatgttcg taaagatgaa aatggaaagc aagaatacta tgtaaaaaaa gggtttaaaa
     9481 ctgaaaaaga agccaagtta cacgaagcaa gaaaaaaggt cgagattgaa gataccggct
     9541 atcataagcc ttctaatgac ttgtttataa cagtgtatga agaatggttg ccttcttata
     9601 ggaatcgtgt acaagaaacg acatatcaac gtacaaaaga ccttttccgc ttgcatatac
     9661 taccaaaatt cgggaataag tccatttcta agattactcc tgttcactgt cagaatgcag
     9721 tcaatgaatg ggctttaaaa ttcaagaata tcaaacagct taagtcttac agctctcaaa
     9781 tatttgagta tgccattttt gcagagctat tacaccgaaa tcctatggca aatacaaatc
     9841 taccaaagcg ggaaaagaaa gaaaatgaaa attacttttc attagaagaa ctaaaagctt
     9901 tttgggatat tctcaataaa gaagagcctt taaaacatat tttaatcttc cagcttttga
     9961 ttactactgg tatacgtaaa ggagagctag ctgctcttca ttggtctgac atttattttg
    10021 aagaacagct aatgtatgtt agaaagagct atgcaactat tcgaaatgaa gagaagaatg
    10081 caagaagaaa aactgtcagg attcaaaaaa atacaaaaaa tatatcttcc gaacgaatat
    10141 taccaattga tgcacaaact attgaaatgt taaaaatctg gaaaaaagaa caagctcgag
    10201 agcttttgca gtttggcata aatacaaata caaaaaatca acttatcttt acctatgtaa
    10261 atgcagacgg tgacattaat caacctttgc atgcggacta ttcaaataat atattgaaac
    10321 gtttagagaa gaaatataaa tttaagcacg ttactattca tggattccgg cacactcacg
    10381 ctactcttct actagaaggt ggagcaagta tcaaagaaac acaagatcga ttgggtcata
    10441 aaaatgctga aactaccctt aacacttatt ctcatgttac tgaaaaagct caacgaaatg
    10501 cgttagaaaa attcattgct tacactggtt tataatattg tcattacctt tctcattacc
    10561 agaacgaaaa aaagacacct tctcgcaatg agaaagtgtc tgaaatcgtt ggtataacaa
    10621 cgattaacgt tttgaaaact gtgaagcttt acgtgctttc ttaagacctg gttttttacg
    10681 ttcgaccata cgtgcgtcac gagtaagtaa gccagcacgt ttaagagcta aacggaagtc
    10741 aggatcaact tctagcaatg cacgagcgat accatgacgg atagctcctg attgtccagc
    10801 gtatcctccg ccgtttacgt taacaaatac gtcatatgcg ccttttgttt cagtaacagc
    10861 gaaaggttga ttgattactt cacgcaagtc agcatgtgga atgtattctt caacatcttt
    10921 tttatttaca gtaattttgc cagttcctgg tactagacgt acgcgagcaa ctgcgttttt
    10981 acgacggcct gtgccgatat attgaacttg tgccaatgaa attccctcct attagattaa
    11041 gtttgtgatg tctaaaactt ctggttgttg tgctgcatgt ggatgttctg ctccaccgta
    11101 tacgttcaat tttgtaaatt gtttgcggcc taaagtgttt ttaggaagca taccttttac
    11161 agaagtttcg atcaaacgac gagaattttt agcacgcaat tcgccagcag tgattgattt
    11221 caatcctcct gggtagttgc tgtgacggta ataaattttg tcagtcgctt ttttacctgt
    11281 taatttcact ttgtctgcat tgatgacgat gacgaaatcg ccagtatcca aatgaggtgt
    11341 gaaagttggt ttatttttac cacgtagtac agatgcaaca actgttgaaa gacgtcccaa
    11401 tggaacatca gttgcgtcta ctacatacca tttacgttct acttcgcctg gtttggccat
    11461 atatgttgta cgcacgttat tttcctccaa tttaagttct gatgtttgac atacacttcg
    11521 agtttccggg gctctttgtg gggcaaacaa taccatttaa catattaccc gctaatctag
    11581 tcaatgtcaa cgtttttctg cttgattgaa aaatttatat gaaactttac ttcttttctt
    11641 tcttttatat aaaagggtcg tttcctgtca aaatgaaaaa caaaaagagg ctgtgacaaa
    11701 agtcttgtca caacccctta ttttgaataa acggtgttct cctgccaact cctcggtcac
    11761 aagtcacaat atccataaaa cataagaagc tgttttttaa aattgtttct tgtgactcgg
    11821 agctgaacgg caggcttaca acctcttggt aaacggtgtt caatcagctg accctcggta
    11881 ttagctcaaa aaacggcaaa atatgaggaa ctattttgac gtttgtttct ctaatactca
    11941 ggtcaaaacg ctgatttcac aacctctttt tcggtcaagt tcaacaacag tttttacgcg
    12001 tcgatctctg gcgctttgcc caaatgagct tggatcatcc agatacgttt ttctgtggct
    12061 gttttgaatt cgataaagat atcttgtgtt ggatcgtcgc cttcttctcc agaaacttcg
    12121 attccttttt ggtaaagatc tgctagataa cgatagcctg caacaagtgt ttccaaacgt
    12181 tctggaatag tacgatccca gcggccaatt tcatcaggga tttttgtgtt atctgcaaat
    12241 tcacgcaatg tagaataagg tgaaccgtct aatgtgatca agcgttcgga tacttcatct
    12301 aactgagcgt tcaaatcatc catgaactca tccatcattg ggtgcaatgt aaggaaacct
    12361 ggtcctctca tgtaccaatg tgtttggtga acaacaacag acatttggct tagatctgca
    12421 actaattgat ttaatacttc tttggtttgt tggaatttca tctttcttgc ctcctaacat
    12481 tttcttacac ttaaaataat agtagacttt caatgaaaag tacaacaaaa tcatttacct
    12541 gagaacgatt ttcagttcgc ttttttatcc gtaaagccaa tcatttttca gaatcagcta
    12601 attcttctcg cagttgagca aattgctgat aagtctgtgc taaatgcttt tgtgtcgaaa
    12661 caaataatgg atagattttc tgatattttt tatgatcagt ttcttgtact ccaacttgct
    12721 ctttcaattc tatcaattcc tgtgcttcac tgatactttg gatctttccg atgcttctcc
    12781 agcctaaaat cacagctcct aatgcggatg cttctgtgac agtagggaaa acaagcgggt
    12841 aacccagtac atcagccatc atctgacgaa aaatcggaga ttgagcaaac ccgcctgtag
    12901 cacggatctc tgtgagctga ttttcctggc aaactccttt taaaatccga taaaggttga
    12961 gacagatccc ttctagaatc gccttcatca tttctttttc tgtatgttgc cgctgtaaac
    13021 cgataaaact gccaaacgca tctgcgtccc aaagcggtgc tcgctcacca agtaaatagg
    13081 gatgaaaaat cagaccattg gcacccgctg gcacaaaagc gatagccgaa agcagtgcat
    13141 catatggatt ttctcctttt tctttagcag attgaacgac ttcctgtaaa aatgtatgcc
    13201 ctgcccaatc aaagatgcca gcaccattgc tagtcgctcc tcccactacc caatgtttct
    13261 gatccagaac ataacaaaac gtctccattt ctggatggaa ctgaggctga gtagtaataa
    13321 aacgaagtgc tccgctcgtt cctacggtca aagcggcgat cgtatcacca aaagcaccta
    13381 accctagatt agaaagcggc ccgtctgccc ctcccaaaac aaatgatata tcatttggca
    13441 aacctagttc tgctgctgtt tttgatggta gacctgttac ttgataggtg gaaggaaaga
    13501 cttgcggcaa ttgctctatt cgaatgttca agtagtcaag cacttcttct gcccattgga
    13561 attcatggat atcaaaatat ccagttcctg aagctgttcc gtagtcgctg atcagttcgc
    13621 caaacaatct ataaaagata tagtctttga taccgacaaa ataagcagtt tttttgaata
    13681 attcaggttg gctgttttgc agccacttga ttttgttcaa tggtgtcatt ggatgaatcg
    13741 gcgtcccagt ctgccgataa aattctttgc caagcacggt atatttcagt ttttctgctt
    13801 cgtccgctgc tcggttatct gcccaagtga tcattcgaga taacggtgtt ttctcttcat
    13861 ccaacaatat caggccatgc atcgcagagg agaaggagac aaattgaatg attgtgtctg
    13921 aaggcaactg tttaattact tcttttataa cggtgcagac accttgaaag atcaattcct
    13981 gatcttgttc tgccatccct tgtgtttctt ggatcaaagg gtagcgaaaa taagcggatg
    14041 ctgcttcttg ggcttcatct gtgaaagcaa ctgctttggt ttgtgtggtt ccgatatcta
    14101 atccgattga aacgttcatt ttttcacctc aaattttatg tatgtacaaa aatttagttt
    14161 aagcttgcgt atcttggttt tgaaaagggg gagatggcgt gttactttat tttatcattg
    14221 gttgctgtat cggatcattt ttatgtttaa ccgcccagcg gattccgtta ggtcactcta
    14281 ttatttatcc tagatctcat tgtgtcaaat gctgtcgttc gctttcttgg tatgaattga
    14341 ttccgattct ctcgatcatc gtccaacgat ttcgctgccg ctattgcagg tgtcggttgc
    14401 ccttctacta tttgttggca gaagccttat gtggtggtct ttttgcctgg ttctttactt
    14461 tttcttccgc aagaaatttt tcgacgttca tatggatgct ttcggctttg ctattttcgt
    14521 tgatggattt attttacttc atggtccatt ctcacacgct attctgttct tgggcaattt
    14581 tatggatttt ttggttgcag gccggcgtct tccaatggca aagtgtgctg ttaacgttta
    14641 tcgtaggcgg tgtgtgcctt cgctatggtc aatcctatct cggagctggc gatactctgt
    14701 tgatgctttc ttggagtggt ggcttgtctc tagaagagtt gcttcagatc ttgtttcttg
    14761 ctagtctgtt tggaatatcc gttttttctg tttactttat ctgtcataaa aaaaagttgg
    14821 aaaagctgcc tttcattcct tttttgagca gtgcgttgct gattgtcctt catcttaggt
    14881 attgaaaaaa ggttgtgaca aacgacttgt cacaacctct gatatatagt gttctcttac
    14941 caaatccttg tgacttggag ctaggcgaca ggttcacaac ctctccacta aatggtgttc
    15001 aatcagctga ccctcagtat tagctcaaaa tgctggtttt acaacctctg atatacggtg
    15061 ttccataagt agctttttcg gcttcttatt tcttgacgcc ccaatgtcga cgaaggaaac
    15121 ctgcacgtcc agaaccttct cgataaagat tgtaatggag cttgtttttc ttgtaataat
    15181 cctgatggta atcttctgca ggataaaatt cgcttcttgg ttcgattttt gtcacgattg
    15241 gcttatcgaa tcgcccgctg tcttgcaatg cttgttttga acgctctgca atatttcgtt
    15301 cttcttcatc attatagtag atcactggac gataagaatc accgcgatca gcgaactgtc
    15361 ctaatgcgtc agtcggatcg gtttgctgcc aatagatttc caccaactga tcataagaaa
    15421 taacttctgg atcatattcg atctctactg cttctgtatg ccctgttgtg cctgttgtta
    15481 cttgttcata agttggattt ggtacatgcc cgccagtata accggatgtc acagaaatga
    15541 tccctggctg ctcttcaaat ggtttgacca tacaccaaaa gcatccgcct gcgaaaactg
    15601 ctttttttgt catataaaat cccccttttt tctcatcata ccttgatttc caaaaggagg
    15661 ataacgtttt gcttgtgaca gaaaaaacgg aaactattat atacgtgata cggcttctga
    15721 taaatgccaa aacgaacgta cttgatcaaa aacggttaaa tcaggaatca catattcagc
    15781 agaaggaaga tttatgtttt cacctattcc caatacatgc atgttaccag ccaaagctgc
    15841 ttgacaacca gcttctgaat cttcaatgac caagcatgca tttggcggta cattcaattg
    15901 ctgtgctcct ttgagaaaaa cttctggatc aggttttgct tttgatactt gcgtgccgtc
    15961 aataaggaca tcaaaataac ttgtcaaatt cgttttttct aaaatgaggc gtgcattttt
    16021 gcttgctgat cccaatccga tcttgatgtt tgtttttttc aagtactcaa gaacagctat
    16081 tgcaccaggc aaaacggatg tttcatccat tttttgaatc gcttgaagat atagattgtt
    16141 tttttgctca gcaaaggctg atttctcttt ttctgagaat ttctgttcca tttttccaaa
    16201 tgacagcaat cgctctagtg actcggttct ggatattcct ttcagttgct cgttttcttt
    16261 ctcagtgaat ggaatggaca gctggttagc taaaaccagc cacgcttcgt aatgataacg
    16321 agctgtgtct acaagaacgc catctaaatc gaatagtgct gctttgattt tcatcgatta
    16381 cctccttgaa aatgaacgac ttgaagtaaa gatgcgatgt ttttagctgt gcgatgaaga
    16441 ttatcagtgg cttgctgtaa agcttcttgt aaaggcacag ctgcaggaag gatcggaaaa
    16501 acggccgaaa tgccaacatc tgctgctttt tcgcttcctt taccaacact tccgcaaata
    16561 gctatcacag gaacgccctc tggtactctt ctagcaatcc caactggcgc ttttcctttt
    16621 agactttgtc catctagacg tccttcgcct acgattacga ggtctgcttg ctgtacccgt
    16681 ttatcgaact ccagtaaatc caatacgtag tcgatgccag agactatttt ccctgaagca
    16741 aatgctaaca gtcctgcacc cattccacca ccagcactac tgcccgatgc ctcgattact
    16801 tgaggggcaa atctcatata aaaatcatgc atggcttggt cagccactgc taattcattt
    16861 tctttcaagc ctttttgtgg tccgaaaaca aaagaagcgc cttcttttcc gcataaaaga
    16921 ttagtaacat cagtaatgac agtgattttg gctttaatca gctcaatagg cacgttcgtt
    16981 ttatcaactg tagcaatttt acctagattt tctcccagtg cttctacttt ttgattatct
    17041 tgatcgtaaa aagcataacc tagaccttct gccatgccga tgccgccatc gttggtacta
    17101 cttccgccaa cgccgatgat gatgtgtcga acaccttgtt gcaacaattg tcgtatcagt
    17161 tctcctactc ctcgcgtgga taacctaagt ggctgttgtt ggtttagtgg gacatctttc
    17221 aatccgacga tttctgccat ttcaaaaatc gcagtttgac cgtctgaagc gtactggatt
    17281 tctttgtttt tgccaaatgc attagtaacg actgctgttt gccgcttcaa ccttaaactg
    17341 tctgctaatg atgaaaccgt tccttctcct ccatcgccta taggtagcag atcatattca
    17401 gcctgaacaa aaacttcttg aaaacctgat tgaatggctt gtgctgcctc taaggctgtc
    17461 agactttctt tgaaagaatc tggcgcaatc agaatacgca ttattttccc ctccgttcca
    17521 agatcagttc tttttccagc tggtattggt ctccattgac aaaaataggt aatgtttctt
    17581 tcgtctcttt ttttattact atttgttttt ttgtcaaaac aaatagcaac ctgtgatttt
    17641 tccaaataaa aggaaaagaa attttttccc atgctgaagg aagtcttgga gaaatagaaa
    17701 gagttccatt cgttactgta atacctgaaa aaccaaatat aactgctagc catagtgctc
    17761 ctaatgaagc agcatgaatt ccgtcatctg atgaatgcgg atctggtcct aaatcaatca
    17821 ggcacgcttc ttggaataaa tgatacgccc attccatttc ttggatacgg caagcctcta
    17881 tggcgtgaat cgctttactc aacgatgagt cgtggattgt tttgttttca tagtaatgca
    17941 aattcttaga aacgattggt tgagaaaaat actcaggtaa taagtataac agcatgacaa
    18001 gatctgcttg cttgctgact tggagttcat ttacttcgct acgggcataa tcaagtaaaa
    18061 tagactggct gccaggacgt tctttgtaag tggtcaaatc aataactggc ttactgagat
    18121 acgtgtcatc ttgcggaatc agctgttctt tgttaggcac tggcaaatat aacttatcta
    18181 aaaaattttg acattctttc aaaaaaagcg gatcgccagc ttgatattta acagctaagg
    18241 ccacattata atgcgccaga tagttcgtat atgcattatt gtcgatatat tctgtgtatt
    18301 catctggacc aatgactctg tgtatctcta aatgatcatc gacttttaca gctctgctca
    18361 gccaaaaagc tgctgtttca tgaatgagcg tttttccctt tgtttctaaa aacgattgat
    18421 cctctgttgc ttggacatac tcttgaacgg cataagcaag gtctgcactg atatgatgtt
    18481 ctgcggcagc tgaagcaact tcttgtcttg ttccggtttt gatattcaat gcagcaaatt
    18541 tgggtgtctc ttcctgacca gtgtgtgcgc tttcccatgg gaaaagtgca ccttgatagt
    18601 gataatctct tgctttttgc cgtgcgcctt ctaatcgatc ataacggtac agcagcaaat
    18661 tctttgctac ttcaggaaac tgatataaaa agaaaggcag gataaaaatt tccgtatccc
    18721 agaaaacatg ccctttataa ccttcgccag ttagtccctt cgcccctaca ctaacacttg
    18781 gatcattgcg gggagtcata atctgtaaat ggtaagcagc gaagtccatc gctaactgat
    18841 cgaaagaaga atcgccttgg atcgttatcc gatgctgatg ccaaaaatct gtccaataag
    18901 acatcgactg ctctaaaatt tctgtataac taacgctttt ctgggcttgg gcaatacttt
    18961 tcttaaacgg gtctgtttgc tgaagagagg tagagagcac gatgatcttc tccagcgtaa
    19021 aggacgtatt ttttctgatc ttttctgaaa aggaagcggt gatccggcga ttttttgctt
    19081 ggaatacacc tgctttattg aatttagctg caatcccaat atcaatttta ctttctgttg
    19141 tctggtagga tgcactgatc aaatcgtcac caaatactcg cagttctttt tcaaccaact
    19201 gctgtgtacc aaaattggtt tgctgtccat cgatacccgt tttcacatct acttggcagt
    19261 cttcggtcaa cggcttgatg accactttca gggctgcctg atggagatta ttttgatcag
    19321 caacacgaca agtaatgact tccacttttt ttccttgagc agtttcaatg agcgcatagc
    19381 gtttaagctc tcccatttct aaatctagtg cccgctcgta tgcaagaact ttcatcgtct
    19441 caagagaaaa atgttcaccg tcgattttta tttcccatct gatgacatct gggagattca
    19501 ctaactcggc tgattctgct cctaaaggtc ggttatatac accagctgca aacattcccc
    19561 ttacttgatt ccgatagtct tcctcctggc ttcctctgat accaaaaaat ccatttccaa
    19621 tggtcatctg actcgcatac ttattgatat actctaaatc aaactgtttt tcttctactt
    19681 tcataaggta ataacttctc ctgtttctgc cgaacgatat aaattttcca ccagattctg
    19741 gatgacatat ccttgccagc catctgcaat catctgagtc tggtcaccga gcgcccgatt
    19801 cacaaaatga gccatactta gttcatgtgg gtcattaact ggcgtggtct tttgtaagga
    19861 aaccagcttt ccatctacat cttcgaatag ttcgcctgga tacagattag cacctgcctg
    19921 atcgccacaa aaagccacat tcagtttgtc ttctctgata ttcaaagcaa acgaagtatt
    19981 cagttggatg atcttgccgt tttctaattc cagtgtgcca aaaattgcat cttctactgt
    20041 gaatttgctt ggatcccatt tcccgaattt ccctgaattt tttttcgtac caattttttg
    20101 gaagctgtac gcccgaacgc tttttacttt aggaaatcca gtaatataca tcgctgtatc
    20161 aagcatgtga atgccataat cgatcaaagg cccgcctcct tgaagttctt tgtcgataaa
    20221 cgttccccag cctggaacac cagaccgacg aagcgcattc gcttctacgt aataaatatc
    20281 cccgagaaca tcaagatgat tcttgatcat tgttgcttcg tcagaaaaac gatgctggaa
    20341 tccataagcc aaaatctgcc cgtttttatc ggctgtctct gccatctttc ttgcttgatc
    20401 agctgtcata gctggtggtt tctcgcacat cactgaagca ttagcttcga gggcagccaa
    20461 tacatgttcg aagtgaaagc gattgggtgt acagacactc acgatatcgg gagaaatctc
    20521 attcagcatt tgtgtaactg atgtatacgc atgagaaaat cctgtacgtc ggcaaaagtc
    20581 ttctgctcgc tggctgactg gatcaaccac agcggccaat tctaagacat ctgatctttt
    20641 ttgataataa gcagcatgta ctttttctgc tacttgtcca gcgcctatga ttgctacttt
    20701 cttcatttat ttctctctcc tttgcttatt tacttattgt tattctttct ctattctttc
    20761 tctaaatcat ttaaatgacg cgctgtttcc agacagtttt tcaagtattg caatgattcc
    20821 tgataaattt tcacttcatt ttctcctcgc aaacgacatt catataccat ataaccttca
    20881 tactcatttt gaagtagcgt ttgaaaatgg cgagtaaaat caattgcgcc agatcctggt
    20941 tggtagcgat gattatctgc taaatggata tgacccacat actctttttg cgtagtcaat
    21001 gcttttgaaa tatcatcttc ttcaatattc atatgataaa aatcagcgat gatctgcaca
    21061 tgtttcaatt gattagagtc aatgtaatga cgcgcatccg ctaacaaatt gatcatatgg
    21121 tcttggtatc ggttcaaagg ctccaaataa ataaccgtac ctgtttcttc ggctacttta
    21181 tctaaataac gaagtgattc gctgactgct ttatagtctc cttctttgct tcggggtgaa
    21241 accatcggcg gtaatcgata agtgaacatt ccccaagctg ctgggataac gattcctttg
    21301 cctccaacct cgcttagtgc acgcaaaatc tcagcaattt gacgtacacc attaagacgc
    21361 cgttcttcga taaagtcacc gatccaccca tcgtagccgt tacaagctgt cactactggg
    21421 atccctgttt ctttgattgc ctgtttgact gtgtcgatat tttctattaa caacttaccg
    21481 tcgatttcgt attcttcaaa tccacagtct ttgataaatc taaatttttc taaaatgtct
    21541 cttgggaaaa atgcttgatc ttgcgtaccg aatttcattg ttctcctcct cggacttgaa
    21601 cgcccatttt gatactttct tctgggtgct ggtctacata acgcatgtaa gcttctgcgc
    21661 tttctgcaaa tggcacaact ggatcaatga tttcttcaca atccaagtaa ccgttcatca
    21721 ataattccca acatgtgtcc tcgattcgtt tacggttcca tcttgggtaa tctgggtttg
    21781 gttcactaga tgctcgagca aagacgattt ttgcattatt gaaatgtgct tcgcgaccaa
    21841 gattaaaccc agctggaaaa ggtttagcaa aggcaacata agaaatgatc ccgccatagc
    21901 cgatccctat taaagaagct tgtaatgcgg cagcactgcc actcgtttca ataactgaat
    21961 ctactccacg tttatctgtc aattccttta gacgaaaacc gacatctgct ttcataggat
    22021 ctagcgtgta atcggcaccg tgtttttctg ctaattctct acgctgcgct atgggatcga
    22081 tcccaacgat ggtagtagca cctgcttttt tagctaattg aatcgcaatt tgcccgatag
    22141 cgcctaaacc gaagattgct acagcatcac ctgctcgaac atgtgcttcc cggataccac
    22201 ctaaagcgaa ttgagcagga tcgtaacata ctgcattttt ccattgatcc aaccgatcaa
    22261 gtttacgtag tttataatta tcaacaccgt ttgtaatcag cgtttcacga atgggtccat
    22321 aagtgcagac taactctcct ttttgatact ctgtcaccaa actgcccgtt tcttcgatcg
    22381 taccgacaat catatttcct aattgaaatt cgccaaatac cacaccgcgt tggcttcctt
    22441 cttctcttgg caagaatagg cgccattctt ctgaaaattc ttcatcgata aatggactta
    22501 atcctctaaa gtcgacaatt tctgttcctg gttttggagt agcgaaaatt actcggatac
    22561 gtacttcgtt cttttcgatc tcacggtctt cgtaatcttt gaatccggct attcttggtt
    22621 ctaatgcaac taattttttc atcttttctt tcctctttct tttcttttta tccttttacg
    22681 ccgccttcga ctaaatcacc tttaataaat cgttcagata acgcatacat aatgatgact
    22741 ggtaaagcgg tcaccaatga agcggccatc attcgtcccc aaatgtagtc tggtgtactg
    22801 aataatgtgt tcaaaccgat tggtaaagta aatttttcgg aggtcgacag gaaaatcgaa
    22861 gcaaataaat aatcattcca cgaaaccata aagcaataaa cgaagacaga aatgattcca
    22921 gaaatagcca atggtataat gatccgaata atgatctgga tccggctaag accatcgatc
    22981 attgctgctt cttcaatatc agtcggaatc gtgtcaaaat aactttttag catgaatatc
    23041 gcagtaggaa gtgtttgaac gaccatcgtg ataatcagtg ctgtattggt atcatacaaa
    23101 cctagaccgg aaatgatttt aaataatgga acaacgatca aaataccgga aaacatataa
    23161 acggtgtaaa agctagcatt gattttcgag cgagctttga agcgtagttt tgacaaagcg
    23221 tatgccccgc tgatcccaat cactactgca gttacggagg ctatcaagga gatcactaaa
    23281 ctatttttga aatacatgat gaatggaaaa atctcagcat tgaaaatatc ctgataatgt
    23341 tcaaaagtcc attctttcgg aaaaatagtt tgtgtcgttg caattgcttc ttgtgaatcc
    23401 ttgaatgatg tcatcagcat gatcaaaaat ggaaacaaat tgacagctaa tagcaacaaa
    23461 atcgtcgcgt aaaataagat tttcctgctc tttttatttt tcatttcaag tccaccttct
    23521 ttcgtgagag caagacgacc gatgcaataa tgatgaacaa tagaatcgat atagctgctg
    23581 cttttccaag atcattgaac gcaaaggctg tctgatacaa ataaacgcct aagatattga
    23641 ctttattcgt cagtagatag acatctgtaa acatatagaa catccagata gatcgtaaaa
    23701 caatcacagt tgctagtact ggtttgattg ccggcagtgt cacgatcttg aacttctgcc
    23761 aagcatttgc accgtccatt tcagctgctt cataaagcgt tttgtcgact gtttgcaaaa
    23821 tagccaaaaa tgaaataaac gcatacggat agtatctcca gatagcaaac aaaacgacca
    23881 taaagaagct agcaactggc tggtcgaacc acagcggtgc ttcgttgaaa agctgcaacc
    23941 tatctactaa cagatcattg acgattccat aactattatt gaacatatat ttccaagcaa
    24001 aaatcaaaga aatagatggc gtaacataac ttaataaaat cagtgaacgc accgttttcc
    24061 taaatctgaa cttgcggtta agcaaaatag ctacggctaa tcctaaagct gtactgcctg
    24121 ctacaactaa gaatgtgtaa gcaactgtta aacctaaaga acgataaaat tcactatcac
    24181 ttaaaatttc aagataattg cttaatccga taaaaacggc gtctgagtta ggattcagcg
    24241 gcatatcgaa aaaactgatc tgaatattgg aaagcattgg aatcagcact aaaacagcta
    24301 ataaaagcat gcttggtgct aaaagtccca tggctaattt taagtcagaa tgtttccttg
    24361 gacgattcat tgaaacctcc ccctgataat ctgccaatag ctatctactt ttttcgaaca
    24421 ccattggcag aacttcttgt tatttcgtat ttttttgtgc gtcagctaaa gcttgatcga
    24481 catcttcgtt tccgacagtt acttggttca ctgcttttcc gatcacacct gagcttgtga
    24541 tatctcccat tttcgtgaaa ttcttttccc cgactagtcc aaagacttgt acttcatcaa
    24601 aagcactagc aatctcagat ggcagctcgc caaaagactt gatgacttca ttttctttgt
    24661 aaccatccag ttcgactact tgtttattca ctggttgagc accaccagga gacatcaaga
    24721 cccacttttc catgtttttc ggttgagata agaaagctgt gaatgcttca gcagcctctt
    24781 tttgtttctc gtctagacca gaagaaatcg tcaatccaga tactgtgcca tagacagctt
    24841 gatctttttg tgttgggata gcaaaaccga tattttctga gttccccgct tcataaacag
    24901 aaggcaaaat gtaagttgaa tagattgcca ttggtgctgt tccattcatg aaagcatctt
    24961 tgatttctgt cacatcatta gagcctggca tcgtgtattg ggataagttt tgataaaacg
    25021 acaatgcttc tctcatcttt tccgtatcga tcgttacctc gccttcatca tccaaaacat
    25081 ttgctccatt tgacaaagca aattgagaga acgcttgctc actcattgtt ccttcagcag
    25141 taggcatagc aattccatat tgtttattct ctttatctgt gaaatgttga gcaattttta
    25201 aaatatcatc ccaattttct ggttctgaaa aaccagcttc tgataatttt tctttgtcat
    25261 accaaatacc ttgaacccag ccgctaattg gtgcagcaat atagcttttt ccatcttctg
    25321 aacgaacaag gtttttggct ccgtcataat aattatcttc tccagcttcc tcgataacgt
    25381 tttggactgc cgtctgatca atcagttcat ctttatccat cactttagca aaatcctgac
    25441 tgacttccat tacttctggt aatttaccgg aacgagctaa cgtaacgatt tttgtattga
    25501 acgctgattc ttctaccggc acttgcttga ttttgatgtc tggattttct ttttcaaaat
    25561 cagcaaccag ttcattgatc acacttaatc gttcctgttc gacagaagag tgcatgaatt
    25621 gaatcgttgt ctgttgtgat tcgttagagc tttgactttc accacaagca gtaagaagaa
    25681 atccagtagc tagcagacag gtagtaacaa taattttttt catttttttc cacctcttat
    25741 tttattattt tcagccacat atattcatac ggctggaata tcaactttcg ttcttgactg
    25801 caatggataa tttgtccaga aaacaggtct aggtaatctc cttttggtaa aagataacct
    25861 gctgattgat tggaaaaatt atggataact aagactttct cttccttcgc ttcgcgaata
    25921 aaactgataa aagaatctgc tgagtccagt aagtaaatca aactattcgg atggaaggct
    25981 ttttcttttt ttcgaataga tagcatctgg gtcaattcac catatactgc tgttcttaat
    26041 ggatctgtct gcaactcttt ctcaatatct gctaacaaaa atttttgccg attgattttt
    26101 cgattctctc ctgttttttc tacgcctttg taatcatttc gacttcccag cacactttga
    26161 atataaattg ccggcacgcc tggtaacgtt accaaaaatg aatgagcagc taaaaagcgt
    26221 ttgcttctcc attccgtcgt atctgttgga gaacttaacg catctagata cgtagcgttt
    26281 atctcatatg gactggtcgt tccatctggg tttttcttgt agtttactcg ggcaccctct
    26341 ttcttcagct gttccactaa ttcaaggatc gttttttccg gcagaatacc tcgtaccgga
    26401 ttcaatccga taccgtcatg ggaggctaaa aaattaaaaa aagttgtttt tttcgatgcg
    26461 aaatcgatgg tttttaacca ttctttcaac ttacttgtat tacccgaacg aatcgcttga
    26521 agggtcaaag gtggtagcga aaactgatag accatctgtg cttcattctc gccattccca
    26581 aaataactga tattatcttt gtgtggcaca tttgtctcag tgatcaatac cgttcctgga
    26641 gctgcttcat cagcgattgc acggaataat tggatgattt tatgcgtttc tggcaggtgt
    26701 atacaagaag ttcctagttc tttccataaa aatcccacag cgtccaagcg aataaatgaa
    26761 gcgccttttt ctaagtaaaa caaaagaata tcgatcattt tcaataaaac ttctggattt
    26821 ttaaaattca agtctacttg atcttcgcta aaagtcgtcc atacgtgttt ttctttcccg
    26881 tttttgtcaa tgaacgttgt aagtaacggg ctggtccttg gtctagtcac gctgcttgta
    26941 tctgtatcca gtggcaaatc aataaaaaaa tctttgtacg tatcattcaa gtttaaatat
    27001 tcctgaaacc aagtactttc ggcagacata tgattgcaga caaagtcgaa cattaaccgt
    27061 gtttcgcttc tcatttcttc tatatcttcc catgtgccca aacggtcgtt gattgcctga
    27121 taatttacaa cggagaaacc atcatcagat gtatatggga aaaatggcaa gaaatgtaca
    27181 actctaaatg tatctgccaa atacttttga taaaatttct taaatgtacg caaagttggt
    27241 tgctgctctt caaaaaattg atccccataa gtaatcaaga aaatatcttc ttcgtcccaa
    27301 tgcagtttgc ctgcctggcc tttcttctgc tgataaacac tgactttttg ttcaatcata
    27361 gaggcggttt tctcagacaa atctgagtcg cgataaatcg cttgtaaaag ttttgtatac
    27421 atcggattcc cctttctttt ttgggagcgt tcccaacgat ttgatcatat tatggaaaac
    27481 gcttactgtc aatagcattt ttttaaataa ttttgatata cacaaaaata caaataagaa
    27541 agcttatgta tcaataatta atagcttctt aaaaaaatat ggtctctcat gatcatgcaa
    27601 gctattccta aattttcaaa atcatagaca aaaaagaatg tctattgtac tatggaatta
    27661 ttctaaaacg ctcccaaaaa ggaggtgcat ataaaattga aacctactat ttatgacatt
    27721 gcgaaaaaag ctaatgtctc aaaatcaact gtctccagag tattaaataa ccagccaaat
    27781 atctctgata aagcaaaagc aagtgtactt caagcaatca aagaattgaa ttatgaaccg
    27841 agcaaaattg caagaggact gtcaggaaat ggttttgacg cgattctagc attatctcat
    27901 cgtacttctc atacgaccta tggcaatcct tttttttcag atgttattca atccatttct
    27961 gccgtttctg aacaaaataa cttcgacttg attctacaaa cggcaaaaac accagaagaa
    28021 gagctagaaa aatgcgcgaa aaagatccaa gaaaaaatga tccgaggaat tattatttta
    28081 agctctacga tggatgaaac acttcttttt gaattggatc aatacaatat tcctattgtg
    28141 gtaatcggaa aagtcggaaa cgactacacg aacatctact cagtggacac ggataatttc
    28201 caggatagtt acgatttggt ggaaacactg atcaagaatg gacaccgtca tatcggctgt
    28261 ctccatgctc cgacagaagt ttatgtatcc aatgatcgtc tcaatggtta ccgtcaatgc
    28321 ttatttgatc accacctaga tatccgtaat gaatggatta tagacggcgg ctttcgctta
    28381 gaagatagca tggcagcaat gaatgattta atgaagctac gagaaagacc aactgcgatt
    28441 ttttctaccg atgcattaaa atcattgagt atttacaaag tggcagaaca atctggaaag
    28501 cagatacctg atgatctttc gatcgcagga ttcagtgatc ccttattgtc ctccctattt
    28561 tcgccacagt tgactcggat agacattccg acaaatcagt taggaatcaa agccacagaa
    28621 cttttattcc aactaatcca ccaccaatac cctgaagaaa aaagaatcat tctttcaacc
    28681 gagattactc tcacagattc gatccaacaa caagaaatgt agaacagaat tataaaagaa
    28741 cagaaacact gaggataagt cagttactct gttctttttt caatcactca tccctcgtta
    28801 ccccctcccg gtattttagc gaatttttct tattttccat ctgttttaga tagctgtgga
    28861 taactataat gtttgtgtta aactattttt tccacaggga agaaaaaact atttttaacc
    28921 acgttgtgta taagttttta tagttcgttt ttttaaacac atttaccctt atttcaatcc
    28981 atgttatttg gggatactta tctctaaaac ataaatagaa taacagacat gtctactggt
    29041 tttgttttat tcgaggaatt attcaataat agctagcaaa atattaaaaa taaccgctct
    29101 atcttcccat caatttattt tgcattcaaa aaatgatcta tctttacttt tgttgattga
    29161 cttctcttgg aagatgatcg ttccgatacc ttaattggat acgaaccgat gttgttaaga
    29221 tcctttccca cttttcttct agttagacaa attattttag acaaaccaaa aaagaggttg
    29281 tgacaataat ttgtcacaac ctctttttta aaatatctat tttgtttgat tgttcattgc
    29341 gtcttctgca gccatttgcg cttcaatatc tgaaatactg tagacatttt cgcttgctac
    29401 accgacttct tttggttcca tgtcacgata acgttgcata ccagtaccag ctgggatgat
    29461 cttacctata ataacatttt ctttcaaacc aagaagagga tctttcttac cgcggatcgc
    29521 agcatccgtc aagacacgag ttgtttcttg gaatgatgca gcagataaga aactatttgt
    29581 ttctaatgat gcttttgtga tacctagtaa gacaggtcga gaagttgcag gaacgcctcc
    29641 tgagactagc gtattgtagt tttgatcttt aaagtctgcg atgtctaata atgtacctgg
    29701 taagatatct gtatcacctg gatccattac acgaacttta cgcagcattt gacgaaccat
    29761 tacttcgata tgcttgtcgc cgatttctac cccttgcata cggtaaacac gttgtacttc
    29821 acgaagcaag tagttttcaa cagataatac atcgcgaact tgcaacaatt gttttggatc
    29881 gattgatcct tctgtcaatg gtgcaccacg gtggatcata tcgccttctg ctactttcat
    29941 acgtgctgta taaggaacag tatacgtacg tgtatctgtt tttcctttga ttgttacttc
    30001 tttggtacgt gttgcaggat cttctgagat atcgatgaca tcccctgtta cttctgtgat
    30061 cactgcttgc cctttcggat tacgtgcttc aaagatttct tggacacgag gaagaccttg
    30121 agtgatatcg tctccggcaa ccccacctgt atggaatgta cgcatcgtca actgagtacc
    30181 aggttcaccg attgattgag cggcgatcgt accaactgct tctccgactt caacgtcaga
    30241 gccagttgca aggttgcgtc cgtaacaatg tttgcagaca ccatgttttg tattacatgt
    30301 gaagacagaa cggattgtca ctgtttcgac acctgcatca acgatttgtt ttgcgatgtc
    30361 ttcggaaatc aattggtttc ctgcaatgat caattcattt gtttcaggat gacgtacttc
    30421 tttacgggtg taacgtccca ctaaacgatc ttctaatgat tcgatgattt catttccctc
    30481 gcggatggct tggatttcaa gacctcggtc agtaccgcaa tcttcttcac ggatgattac
    30541 atcttgggcc acgtcaacca gacgacgagt caagtaacct gaatcggcag tcttcaaggc
    30601 tgtatcggtc atccctttac gagccccgtg agttgagata aacatttcca agacggatag
    30661 tccttcacgg aagttcgaaa tgattggcaa ttccatgatt cgaccattag gagcggccat
    30721 caaaccacgc attccggcaa gctgtgtaaa gttggaaatg ttaccacgag ctccagaatc
    30781 agacatcatg aagattggat ttttcgcttc caaaccttcc atcagttttt gttggatgct
    30841 gtcttttgca gcattccaaa cagcaatgac acgttcataa cgttcgtcat ctgtgatcag
    30901 accacgacgg aattgttttg taatagtttc aacttgttta tgggcatttt caatgatttc
    30961 ttgtttttca ttcagtacac tgatgtcagc gatccctact gtgataccgg catgtgtcga
    31021 atgtttgtac cctaagtctt tcatacggtc aagcatctta gaagtttctg ttactttgaa
    31081 tcgtttgaag acttctgcga tgatgtttcc taagtttttc ttcttgaacg gtaatactaa
    31141 ttcttgctct ttgatatgtg caggaatatc agtaccagct tcaacaaagt atttgtccgg
    31201 tgtttggacg gtcaagttga agtctgttgg ttcattcaga taagggaatt caggaggcat
    31261 gatctcgttg aagatgatct taccaactgt tgtgatcatc gtacgttctt tttgccattc
    31321 agtaaatggt ttttcaccta atgagttagg gttcacaccg atacgtgaat gtaaatgaac
    31381 atatccgttg cgccatgcga taacggcttc gttttgatct ctgaagacca ttccttcgcc
    31441 ttcgcgtcct tcttcttcca ttgtcaagta gtagttacct agaaccatat cttgagaagg
    31501 agtaacaact ggtttaccat ctttagggtt caagatgttt tgagcagcta acatcaacat
    31561 acgtgcttct gcttgtgctt cttcattcaa tggaacgtgg acagccattt ggtctccatc
    31621 gaaatcggca ttataagctt cacatacaag tggatgcaaa cggattgcgc gtccttgtac
    31681 taaaactggt tcgaatgctt ggataccaag tcggtgaagc gtaggtgccc ggttcagcaa
    31741 tactgggtgt tccttgatta cgtcttctaa aacatcccat acttcgtctt cttgacgttc
    31801 gattttacgt ttcgcatttt tgatgtttga agctagttcg cgttgaacaa gctcacgcat
    31861 cacgaatggt ttgaataatt caatggccat ttcttttgga agtccacatt ggtacatctt
    31921 caagaaaggt ccaacaacga taacggaacg acctgagtag tcgacacgtt tacctagcaa
    31981 gttttgacgg aaacgacctt gtttcccttt caacatatga gaaagagatt tcaatgggcg
    32041 gttacctggt ccagtaactg gacgtccgcg acgaccgtta tcgatcaatg catctactgc
    32101 ttcttgcagc atacgttttt cattttgaac gatgattcca ggtgcattca agtctaacaa
    32161 acgtttcaaa cggttattac ggttgattac acgacggtaa agatcattca agtcacttgt
    32221 tgcaaaacgt ccaccttcta attgaaccat tgggcgtaaa tctggtggaa taacagggat
    32281 aacgtccata accatccagc ttggttgatt tcctgaagcg cggaatgctt ctaaaatatc
    32341 caaacggcgg attgcacgtg tacgtttttg tccttgtgca gatttcaatt cttctttcaa
    32401 ttcggctgct tctgcatcta gatcaacatt atcaagtaat tgtttaatcg cttctgcacc
    32461 catagcagct tggaattctt gtccatattg ttcacgtttt tcgcgatatt cgcgttctgt
    32521 caacaattgt tttttctcta aattcgtatt tcctggttcg atgactacat atgaagcaaa
    32581 ataaatgatt tcttccaaag cgcgtgggct catgtctaat acaagaccca tacgtgaagg
    32641 aatcccttta aagtaccaga tatgtgaaac aggagctgct aattcaatat gtcccatgcg
    32701 ttcacggcga actttcgaac gagtaacttc cactccgcat cgatcacaaa cgattccttt
    32761 ataacggata cgtttgtatt ttccgcaggc acattcccag tcttttgtag gaccaaaaat
    32821 ccgctcacag aacaaccctt cgcgttcagg ttttaaggta cgatagttaa tcgtctcagg
    32881 tttttttact tcaccgtaag accagcttct gattttttcg ggagaagcca aacctatttg
    32941 catactttcg aatttattta catcgatcaa aaggggttcc ctccatttca tttaatggca
    33001 aaccgcaaga cggctgatct tcgaccgcct tgcagctcat agaccggatt agtctaaatt
    33061 atcttctgcg gtttcaaagt tttggatgac atcgtctttt tcatcttcca cttgttcagc
    33121 agctttcttt tctaattctt tggcagtttg ctgttcggca aattttgtca aagcatcaac
    33181 agtgatcaaa tcgtcgtctt catcgtccat gtcgcgaagt tcgatctctg tttcttcgat
    33241 atccaagaca cgcatgtcca gacctagtga ttgcaattct ttcaccaata cgcggaatga
    33301 ttccggtaca cctggttttg gaattggttc acccttgacg attgcttcgt atgttttcac
    33361 acgtccgact acgtcatctg atttgtatgt caagatttct tgtaaagtat aagccgcacc
    33421 gtaagcttcc agtgcccata cttccatttc accaaaacgt tgtccaccaa attgagcttt
    33481 acctcccaat ggttgttgtg taaccaatga gtaaggtccg attgaacgtg catgcaattt
    33541 gtcatcgacc atgtgagcaa gtttgatcat gtacatcaca ccgacagaca cacgtccatc
    33601 aaatggttca cctgtacgtc cgtcgtacag aacagttttc gcatcgctgg ccataccggc
    33661 ttcacgaact gtttcccata cgtcttcatc acttgcacca tcaaatactg gcgttgctac
    33721 gtggatacct aactgacgtg cagccatacc caagtgtaat tccaatactt gcccgatgtt
    33781 catacgtgaa ggcaccccta aaggattcag catgatatcg attggtgttc catcaggtag
    33841 gaatggcata tcttcttccg gcataatacg ggatacaacc cctttatttc cgtgacgacc
    33901 ggccatttta tccccttcat ggattttacg tttttgaacg atataaacac gaactaacat
    33961 gtttacgcct ggtgacaatt catctccagc ttcacgagta aagattttca catcgtgaac
    34021 gataccgccg ccgccatgtg gtacacgtaa tgatgtatcg cggacttcac gagctttttc
    34081 gccaaagatc gcatgcaata gacgttcttc tgcagataat tctgttaccc cttttggcgt
    34141 gactttccct acaagcaaat cgccatcttg gacttctgca ccaatacgga tgatacccat
    34201 ttcgtctaaa tcttttaatg cgtcttcccc gacattaggg atttcgcgtg tgatttcttc
    34261 aggtcctaat tttgtatcgc gggcttctga ttcatattct tcaatatgga tagaagtgta
    34321 tacgtcatct tttaccaaac gacggctcat gatgatcgca tcttcatagt tgtacccttc
    34381 ccaagtcatg aaaccaacta aaacgttttg gcctagagcc atttcgcctt gttccataga
    34441 tggtccgtct gccagagtat cccctttttc gactttctca cccaaatgaa cgattggacg
    34501 ttggttgtag cttgttccgg agtttgaacg acggaatttt gttacatcgt atttatctaa
    34561 tgcaccgttg tcgcggcgta cgcggatttc agaagcatca acgtattcaa cgacaccgtc
    34621 atgtttacat aacaaagcag ctcctgagtc atgcgctgat ttgtattcca taccagtacc
    34681 aacccatggt gcttgaggat tgatcaacgg cactgcttga cgctgcatgt tggcacccat
    34741 caaggcacgg ttggagtcat cgttttccaa gaaaggaata catgctgtcg caacggcaac
    34801 tacttgttta ggtgatacgt ccatgtagtc tactttatca atagatactt ctaagttttc
    34861 actttgagca cgagccataa caacgtcttg agcaaatgtt ccatcttcat tcaatgggct
    34921 gtttgcttgg gctacgatgt aatggtcttc gatatcagct gtcaagtagt cgatttggtc
    34981 agtaacacga cctgtttcac ggtctacacg acgataaggt gtttcgataa atccgaattt
    35041 atttactttc gcataactgg acaaactatt gatcaacccg atgtttgggc cttcaggagt
    35101 ttcgatcgga cacatacggc catagtggga gtagtgaacg tcacgaactt cataaccggc
    35161 gcggtcacga gtcaatccgc caggtcctaa ggctgaaaga cgacgtttgt gtgtcaattc
    35221 gccaagaggg tttgtttgat ccatgaactg tgacaactga gaagaaccaa agaattcttt
    35281 gatacttgcc acaactggac ggatattgat caattgttga ggtgtcaatg tttcagtatc
    35341 ttggatcgac attctttcac gaaccacacg ttccatacgt gctagcccga tacggaattg
    35401 gttttgtaaa agttcaccga ctgaacggat acgacgattt cctaagtggt cgatatcatc
    35461 gacattgccg ataccttcca tcaagttcaa gaaatagctc attgaagcaa tgatgtcggc
    35521 tggacgaacc gtttttacag ctgcttctgg atagccgttt ccgatcacgt ttacttcacg
    35581 ttcagggtct cttggtgaaa atactttgat gacttggaca gtcattggat ctgtgaccac
    35641 accgtcttct gatggatagt aagtcactga atttaggcca ttatcaatga aaggagctag
    35701 tgtttccatg acttggtgag tcagaactgt tcctttttca acgatgatct cgcctgtttc
    35761 tggatcaact aatgtttcag ctaatgtcag attcaataga cgtgttttca aatctaattt
    35821 tttgtttact ttgtaacgac caacgtttgc taagtcgtaa cgttttggat cgaagaaacg
    35881 agcattcagc aagttacgtg agctgtctgc tgtttttggt tcgcctggac gcaaacgttc
    35941 gtaaacgtct ttcaaacctt cttctgttct tgagtcgctt gcgtttttat gcaagtcttt
    36001 ttcgactgta ttgcgtaatg tttcgctgtc gccgaagatt tcaaagatcg tatcatcgga
    36061 accaaatcct aacgcacgaa ctaatacggt taaaggaatc ttacgtgtac ggtcgatacg
    36121 tacataagag atgtcttttg catcagtttc catttctagc caagctccac ggttagggat
    36181 aacagtagaa ccgaatcctt cttttccgtt tttgtcgact tttccatgga aatagacacc
    36241 tggtgaacgg actaactgag aaacgattac tcgctctgcc ccgttgatga tgaatgttcc
    36301 ttgttctgtc attaatggga aatcaccgaa gaatacttct tgtgctttga tttcgccagt
    36361 ctcgcggttt gtcaaacgca atgttacatg taatggtgca gagtagtttg catcatgcgc
    36421 acgagcttct gctactgtat actttggttc tttcaattca taatctacaa attccaaaga
    36481 tagattgcca ttgaagtcat cgattggcaa aatgtcttca aacatttctc taagtccctc
    36541 atctaagaac cattggtaag agtctgtttg aatttcgatc aaatttggta attccaacac
    36601 ttcactgatt cttgcgaaac ttctacgttc gcgatgtttt ccgtatttta ctacgtgtcc
    36661 agccaagctc ttcacccctc taaaatgtca ttcaaaaaga ctacccgatc aattgttaca
    36721 ataatatttc aaatattatt tttaggtaac aacagacatt ttttgcggct tttaggcaaa
    36781 aaaaaaccaa aaatagaaca ttctcttgaa accttacttc gtgagcttct acttttgttt
    36841 ccttatatta aagccgaaac ggacaatatt tcgtttctgc cttttcttca tcagatagtt
    36901 tttgcaactt catatactac acttattttc tataaaagtc aacacttcct ggcgtttctt
    36961 ctgctttttc gcaacatttt cttaatgttt tcaattttat gttttcaatt atctgacaat
    37021 tcttaattga cagacctttt tatctcggtt atactttaga aaattcattt tgtggaggaa
    37081 cttctatgcg aaccaacatc aaccaaatcg caccaacaca actgaataac ttttacttta
    37141 gctactttag ataggtttgt attcatcatg atggatacaa acgttaaacg acagtttgta
    37201 tctatgatgg ctaaagaact ttgtattgca gcaaaaagcc acgtagacaa aatgctaagt
    37261 ggctgagcag tgaaaataca aagtaaaagt atcaagcatt ctactttttc aatttatttt
    37321 ccgacttttc aatccatttg gacattttgt ctgaatggat tttttgtttc ccaaaattaa
    37381 ggaggaaaaa gaaatgaaaa aaaatataat catcggatta ggcgctgccc tgctactagc
    37441 tttaggaggt tgcgcacaaa aagacaattc cgcagaggca tcagatggac aaaaaacaat
    37501 tggcgtattg caacttgtac aacataactc gttagatgct gcctataaag gattcaaaga
    37561 aggattagca gaagaagggt ataaagaagg cgataatcta acaatcgatt atcaaaatgc
    37621 ccaaaacaat caagacaacc tgaaaagtat gagtgagaaa ctcgtcaaag aagatcctga
    37681 tttgctactt ggaatcgcca ctccagcagc acaatcttta ttaaatgaaa caaccactat
    37741 cccgatgact gtcacagcgg tcacagactt ggaacaagcc catttagtca catccaatga
    37801 aaaacctggc ggcaatgtga ctggcacgac agatatggtg ccaatcgaaa aacaaatcgg
    37861 cttactgtta aatatcgtgc cagacgccaa aacaatcggc atcatgtata acaacggtga
    37921 agcaaactct aagatccaag ctgatttggc agaaaaagaa ttgaaaaaag cgggcgtcaa
    37981 agtcaaagta ctgacagcaa attccacaaa tgatgtccaa caagtgacga aaagtctagc
    38041 taaagatgta gatggaatct atattcctac tgataacacg ttcgcaagtg cggcaagtgt
    38101 tgtcggtgaa gtcgcaaaag aaacaaaaac acctgtcgtt gctggcgcag ttgatcaagt
    38161 caaagacggc ggtctagcaa cagtaggaat cgactacgaa agcttgggca aacaaacagg
    38221 gatcatggca gcaaaaatct tagacggcaa gaatccaagt gaacttcctg tcgaaacagc
    38281 gaatgatttg gaactgtatg tcaataaaga catggccaaa gcattaggca tcaatccaga
    38341 cagcatcaaa gaagctgaat aaaacacagg aggattctca tgacaaattt gctctctctt
    38401 ttcttatcat ctgcatcgca aggggtctta tgggccctac ttgcgattgg tgtatacgtc
    38461 actttccgta tcttggacat tgccgacttg actgctgaag gaagctttcc tttaggtgca
    38521 gggatcgcgg cagtaagtat tacaaatggc tatcatccat tgacagcttg tcttctaggc
    38581 tttttagggg gtgcggcagc agggcttgtt tccggactgc ttcacacaaa attgaaaatt
    38641 cccgcccttc ttgcagggat catcaccatg actggtttat attctgtcac atcccgtgtg
    38701 atgggtgcgc cgaacatctc tttattgggt cagccaacga tctttcattg ggtcgagtca
    38761 ttcggcataa caaaagacaa cgctgtactg atcgtcggtc tgctttttgc tttgatcgtt
    38821 gtatgtttac ttgttttgtt cttacgtaca gaaacaggac ttgctattcg tgcaactggc
    38881 gataatctgg caatgagcga agcaaacggg atcaacacag ataatatgaa gatcatcggt
    38941 tatatgattt ctaatggatg cattgctctt tctggctcct tacttgcgca aaacaacggc
    39001 tttgctgatt taaattctgg tattggaacg atcgtaattg gacttgcttc tattatcatc
    39061 gcagaagttt tgttacgtaa ccagccacta tttctccgtc tcttgactgt cgttttaggt
    39121 gcggtcattt atcgttttat tttagcgtta gtcttcgaac tgaatgttga accaagtgat
    39181 tcgaaacttg cttctgcttt cgtgctagtg atctgtcttt ctttcccgca gatccagcaa
    39241 aaattccatt tacctcaaaa aaagaaaggc ggtagtgtca aatgaatgac gtcttaacat
    39301 tggaaaatct ccatcatacc tttgaaaaag gaacgatcaa cgaaaatcat gtcctgaaag
    39361 gcattaatct ttccttagat cccggcgact tcgtgacgat catcggtgga aatggggctg
    39421 gtaaatctac tttgctgaac agcatcgccg gtacattttc tgtcgatcag ggtcaaattt
    39481 tattgaatgg aaaaaatatc acgaaaaaat ctgttgtcga acgttcgaaa tccatcagtc
    39541 gcgtattcca agatcctaaa ctagggacag ccgttcgttt aacggtagaa gaaaatcttg
    39601 ctcttgctat gaaacgagga aaaaaacgcg gcttcttccg tggtgtcaaa ccacaagacc
    39661 gaacattttt caaagaccag ctggttcggc taaatttggg gttggaaaat cgtttatcct
    39721 cagaaatcgg tttgctgtct ggaggacaac gtcaagctat tacgttattg atggccacat
    39781 tacagcgtcc agaactgatc ttattagatg aacacactgc agcgcttgac cctgctactt
    39841 ccaaaacggt gatggaattg acagaaaagc tgatcactga gcagaatctc actgctttta
    39901 tggtcactca tgacatggaa gacgcgatcc gctatgggag tcgactgatc atgctgcatc
    39961 aaggaaggat cgtcgtagat atccagcaag aggaaaaaca gcagctcaca gtgccccaat
    40021 tattagcgtt gttcaaacaa aacagcggca cggcattaaa agacgatcaa gtacttttat
    40081 cttcataatc agaaagagga tgggacaaaa aatgaacgcc caccctcttc ttttttctct
    40141 ttccttcttt cgtttttgct ttatgtatca accgttgttt cttcgaaaac tattttgttc
    40201 tctccttctt cctgatagaa tggatgggaa aggagcttga tgccatgaca acgaaagaca
    40261 aggtacttca attattgaaa aagacaccag attttctttc cggagaaaag ttggcacagc
    40321 aactggaagt ttcccggaca agtatttgga aagccatcaa agaattggaa aaggcaggct
    40381 atcgttttga gcacgggcct acagggtatc gttacctgcc ctctgacgta ctagatgcaa
    40441 aagaaatcca gcaagagcta cggtcgttta ttcctgattt gaaagtacaa acgatgttga
    40501 cttctgaatc tacgatgaaa gatgccaaac tggcagcagc tcaaggagat tctgtttcga
    40561 agttatttgt tgctgataca caagaacaag ctcgtggacg ttttggacgg ccttttttcg
    40621 ctgaacctgg acgaggaatc tacatgagtc tcttgctcca tccagacaaa cggtttgatg
    40681 aattgcctca atatacgatc atcgcagcca gtgcctgtgt gaaagccatt gaatctgtaa
    40741 ccggtcgttc tgcttcgatc aaatgggtca atgatatcta catggatgga aaaaaagtct
    40801 gtgggatttt atctgaagca gtcagtgata tggaaagcgg caggatcagt catatcatca
    40861 ttgggatcgg gcttaatttt tctgttcctc aaaatcattt tccagctgat ctccaagaaa
    40921 aagccatctc tgtctttccc gacggacaag ctagtgtcac aaggaatcag ctaatcagca
    40981 aaatctggca agaattgttc acttatcttt ctcacccaga aaaagcgttg gacttctata
    41041 aagaaaaatc attcgtattg ggaaaaaccg tgtcgttcaa tcaaaaagga acgacttatg
    41101 tcggtcttgc aaaagaaatc ggtgatcatg gcgaactgat cgtccaaaca aatgaacaga
    41161 agatgagttt gtcttctggc gaaatcagtc tcacatctat ccgtacgcaa taattatcag
    41221 atttcacaaa aggacacctg atctcataca gtgtcctttt ttctgaattc tcttcaaaag
    41281 actctcctta ttctatcagc tgacagtttc tctatcctct agaaaaaggt attctaaaaa
    41341 tatctataaa gcatggtaca ctttctttaa atgaatcaat gcaaaaaaca agagagtcat
    41401 cagtttacac gatcaaaaga gatagcaggc cgcttccttt ttcaatctta tagatagacc
    41461 atgggtaacg ataaaaacaa aaaagaactt gcgacttatt ttgccgtaag ttctttttta
    41521 gctagtgcct taatctctta atcccaacaa acgtctcgca tagatcacct gaccagagtg
    41581 catagcagca tcatcaataa tagaaacgag acgcattcct cgtgtgactg gtggtgtcca
    41641 gttctcgtca attacttcat ctagctcgga aggttccagc cttcttaagt aagaaatagc
    41701 aaaatcagca gctcctcgca agtatcctag cagatcttct atttgattag cttggatctg
    41761 ctttgcttgt tccagactat gcttccaatc ttgttcgtta tctgctatcg tcatcggaaa
    41821 ccggctttcc cagtcttgac tatgccaaat tggagaagtt cctgctaaat cagcaatctg
    41881 gaaatctaat tctcgagcgg tatgccaagc cagccaagtc attgatttga tctgcggggc
    41941 tctatcttcc actggaaaac gattggcctc ctcaatagag acgccttcaa acaaacgttc
    42001 aaaccgatcg tatgcccgat ccacactctc gcatagcata tctaaataat ccatttattt
    42061 tcctcctttt tatttatcct acttactttt tcctaattct aggataccta cttatatgtt
    42121 actacacacc cttatttttt acactttaac agtcaccatc atcaatagtt tggagattga
    42181 aagaaaaaga tagtgtcgaa ctatcaattg aacaagaagc aacaatatga acgtttcatt
    42241 aatattcttt aaataaacaa tgatttttca aacgaacagt ttaagactag gagaaaaaga
    42301 gtataatagg aaaatgtttg aaaggaaagg aaggggaaaa atgaaaaaaa gggcaagttt
    42361 actagtccta gttgctttgt tgttcaatat cttttcgtat ggtgtggccg tttcagcgga
    42421 aacatacatt cctaaagacc ctgggaatat ttataatgat ctaactggta actatggctt
    42481 gttaggaatt gctagtcaat tccacgtatt tgctaaaaat aagacaactg tgaatgcaca
    42541 tacagacgga aatatagcaa cacatgaact tgatgcaaaa aataattttg gtacagagct
    42601 tcatgaaggt ttattgaacc aagaaataaa ttatatacaa aaaactgatt ctttcgctaa
    42661 ttcttcaggc atacctacag gtagcacaag aatgaataaa tttgtcgttg gcagtaacgt
    42721 ggatgtagca cttcaagatg gtcatcctac agtcaatgga tccaggcttg atcacttaac
    42781 tccagatgat ttttataaag atcgtgctgg gcatgaatat attgatttcg agcaggaatt
    42841 tgctaaattg aatgtagcag caaatgatct tgcaacgatc actccagcta aaacatatac
    42901 tgctgcagat tttccagaca tgaacaatcg gacaatcgat ttaactgatt tatcagatac
    42961 cgggttttta ttggtaaata tcgatgcaga agtgttaacg atgaatacgc cacttcaaat
    43021 cattaatcca aatgatcaag tagttgtatt taatgtcatc aacagttctt ctgcattgaa
    43081 cgtacagtca ccaatcaaat acaatgatcg ttcaaatcat gaaacagaag atttttcgga
    43141 tgcgaatatc tcttggaatt ttggaaatga gatgactgat ttgacaatca gtgctccttt
    43201 tcaaggaaca atccttgcgc ctaatgctac tatccgcatt actcaaaacc aagacggaac
    43261 gattatagga acaaatgtga ttttagatgc agctaccaat cgttgggatc ctaacgaaat
    43321 atttatcagc gacaatggta cagatacgac agacacaagc gacagtacag atacatctac
    43381 cacaagtgat agctcagatt catca
//
LOCUS       DANKEF010000020        40874 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000020 DANKEF010000000
VERSION     DANKEF010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40874)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40874)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40874
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.020"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(21..251)
                     /locus_tag="PWZ48_001225"
     CDS             complement(21..251)
                     /locus_tag="PWZ48_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260894.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            complement(687..1253)
                     /locus_tag="PWZ48_001226"
     CDS             complement(687..1253)
                     /locus_tag="PWZ48_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2260895.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(1253..2209)
                     /locus_tag="PWZ48_001227"
     CDS             complement(1253..2209)
                     /locus_tag="PWZ48_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDL2260896.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(2209..2865)
                     /locus_tag="PWZ48_001228"
     CDS             complement(2209..2865)
                     /locus_tag="PWZ48_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2260897.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            3235..5253
                     /locus_tag="PWZ48_001229"
     CDS             3235..5253
                     /locus_tag="PWZ48_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2260898.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5323..5529
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5680..8094
                     /locus_tag="PWZ48_001230"
     CDS             5680..8094
                     /locus_tag="PWZ48_001230"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDL2260899.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8442..9995
                     /locus_tag="PWZ48_001231"
     CDS             8442..9995
                     /locus_tag="PWZ48_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2260900.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10376..12160)
                     /locus_tag="PWZ48_001232"
     CDS             complement(10376..12160)
                     /locus_tag="PWZ48_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260901.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(12175..13896)
                     /locus_tag="PWZ48_001233"
     CDS             complement(12175..13896)
                     /locus_tag="PWZ48_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260902.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13996..14757
                     /locus_tag="PWZ48_001234"
     CDS             13996..14757
                     /locus_tag="PWZ48_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2260903.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15724..15992
                     /locus_tag="PWZ48_001235"
                     /pseudo
     CDS             15724..15992
                     /locus_tag="PWZ48_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16664..20065
                     /locus_tag="PWZ48_001236"
     CDS             16664..20065
                     /locus_tag="PWZ48_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2260904.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGTYQLVETQAPIGYELDATPIEFEIERSQTAVVELTKE
                     NRLTPGGVVLTKIDDQSGEILQGAVFELQNREGETLQTGLTTGEDGKLAIDGLAPGAY
                     QLVETQAPTGYELDATPIEFEIERSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQG
                     AVFELQDANGTVLQSGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIE
                     RSQTAVVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADG
                     KLAIDGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIR
                     GNNPTSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(20489..20659)
                     /locus_tag="PWZ48_001237"
     CDS             complement(20489..20659)
                     /locus_tag="PWZ48_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2260905.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(20853..21032)
                     /locus_tag="PWZ48_001238"
     CDS             complement(20853..21032)
                     /locus_tag="PWZ48_001238"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260906.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            complement(21042..21266)
                     /locus_tag="PWZ48_001239"
     CDS             complement(21042..21266)
                     /locus_tag="PWZ48_001239"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260907.1"
                     /translation="MNGSIKKILRSIDKNLMITEVSYETLQKKKTLIVAAVLFPAPCV
                     CRNCGSAVVDGNRKLMVVKNKKKETIVHFE"
     gene            21419..22531
                     /locus_tag="PWZ48_001240"
     CDS             21419..22531
                     /locus_tag="PWZ48_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2260908.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(22609..22902)
                     /locus_tag="PWZ48_001241"
     CDS             complement(22609..22902)
                     /locus_tag="PWZ48_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDL2260909.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            23070..23384
                     /locus_tag="PWZ48_001242"
     CDS             23070..23384
                     /locus_tag="PWZ48_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HDL2260910.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            23405..25384
                     /locus_tag="PWZ48_001243"
     CDS             23405..25384
                     /locus_tag="PWZ48_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2260911.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            25397..25723
                     /locus_tag="PWZ48_001244"
     CDS             25397..25723
                     /locus_tag="PWZ48_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HDL2260912.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            25739..26257
                     /locus_tag="PWZ48_001245"
     CDS             25739..26257
                     /locus_tag="PWZ48_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260913.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            26257..27609
                     /gene="celB"
                     /locus_tag="PWZ48_001246"
     CDS             26257..27609
                     /gene="celB"
                     /locus_tag="PWZ48_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2260914.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(27693..29198)
                     /locus_tag="PWZ48_001247"
     CDS             complement(27693..29198)
                     /locus_tag="PWZ48_001247"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HDL2260915.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(29290..29880)
                     /locus_tag="PWZ48_001248"
     CDS             complement(29290..29880)
                     /locus_tag="PWZ48_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2260916.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            30059..31705
                     /locus_tag="PWZ48_001249"
     CDS             30059..31705
                     /locus_tag="PWZ48_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2260917.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            31754..32473
                     /locus_tag="PWZ48_001250"
     CDS             31754..32473
                     /locus_tag="PWZ48_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HDL2260918.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            32952..33899
                     /locus_tag="PWZ48_001251"
     CDS             32952..33899
                     /locus_tag="PWZ48_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2260919.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            33911..34831
                     /locus_tag="PWZ48_001252"
     CDS             33911..34831
                     /locus_tag="PWZ48_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2260920.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            34872..36329
                     /locus_tag="PWZ48_001253"
     CDS             34872..36329
                     /locus_tag="PWZ48_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2260921.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            36674..37288
                     /locus_tag="PWZ48_001254"
     CDS             36674..37288
                     /locus_tag="PWZ48_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2260922.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            37281..39005
                     /locus_tag="PWZ48_001255"
     CDS             37281..39005
                     /locus_tag="PWZ48_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2260923.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            39002..40453
                     /locus_tag="PWZ48_001256"
     CDS             39002..40453
                     /locus_tag="PWZ48_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2260924.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
BASE COUNT        13309 a         7097 c         8248 g        12220 t
ORIGIN      
        1 ctcattaaaa agcataacca ttactccgat ctgtttttaa aattagtttc tacctctaat
       61 atatatcgag tttttatttt gccttcagag aatactgttt ctttttttgc agttacaggt
      121 tgtttatttt cggaaaaagc taatatagct aagattgaaa catccatctg gaatttatct
      181 tttttactgc tttgatcata aaagtctgca tattcatcac tgatattttt tctaataaat
      241 tcttccatca taaaaatcac ctcaaaacga ttatacgaac ttacgttctt gttgtaaagc
      301 gatatttgag gggcaaaaaa ggggcataat ttgactgata ttaagttagc tattgatata
      361 tgaatgacat acaaaaatat aaaatctttt aaaaatgctt atatatcaat gtttttgcca
      421 ttcttttata tatctgttat atgtcaaaaa aatgtgttca atttcaattt gtatttgaat
      481 aaagaggttg tgaaaaagct gtcctgcatc aagtaataag aacagccaat caaaaacagc
      541 ttctcatgtt tttgatttgg ttggacttat taccgcagat ataagctttt gagcaccgtg
      601 aataaagagg ttatgaaatc aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca
      661 tatcatttat gttttgcgtt ttttatttat ctttttttct caatacaata taaaataggc
      721 ggctggttct tttggttgat aaaccgataa ttcagcacat tatatgtttc ctgtggcagt
      781 ttggaacaaa aagaatcaac catatccagt tctttttctc caccttcatg accatagtag
      841 acgacaagga tcaatcgtcc gcgaggcaca agtcgtttca atatttcttc catagctgtg
      901 cgtgtagtct ctggcatagt gatgattgct ttatcacttt ttgggagata ccctagattg
      961 aaaatccctg ctttgagatt ttgttgttca gctatcactt ctcctaacgt ttcgtggcct
     1021 tgtagaaaca atgagacacg ttcttctaac tgacgctgtt ctagcctttc acgagtagca
     1081 tcgattgctt gttgctggat atcaaaacta tagacatgac cagtttttcc gatgtgttct
     1141 gctagaaaaa ccgtatcgtg gccattgccc atcgttgcat ctacgacgtg atcccctggt
     1201 tgcaagattt cttgcagtaa tgtatggctg aaatgcaaag cagtttgcaa catcagacgc
     1261 tcaccttttt ccgtacatca tattttcctt ggaagctatc tctgcgaaga agttcttcat
     1321 caattgcatt caagacttcc cattttttca agctccacat tggtccaatc aaggaatccc
     1381 acggcgcatc ccctgtcaaa cgatggatga tgatctcttt tggtatcatt tccagctggt
     1441 cgcaaatgac attgacatag tcaggacggc tcattaattg aaggcgacct tcatgatagt
     1501 cccgcaacat tctcgttttg cgcatcagat gcatcaaatg gagcttgatt ccttggatat
     1561 ctgaatctaa aatggtgcgt ctgacatttt ctttcatcat ttctaaactt tcgcctggta
     1621 atccgttgat caaatgcgta caaacacgga tattatgttt gcgtaattta gccacgccat
     1681 ctaaataagt ttgataatca tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg
     1741 tttgcaatcc aagttctacc cataaataca tccgctcgtt cagttcagca aggtaatcaa
     1801 ctacttcatc tggcagacaa tccggtcgtg taccgactga taatcctacg acacctggta
     1861 aattgactac ttgctcaaat cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa
     1921 aattttggaa ataaacgatg tattgttgga catgaggcca tttttgatgc atcatgtcga
     1981 tttctttctg gaattggatt ggaagagggt cttcaggtgc aacgatcatg tctcccgaac
     2041 cagatacact gcaaaaagta caaccgccat gggcaacagt cccatctcga ttcggacagt
     2101 caaatcctcc atcgatcggc accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt
     2161 aattccaaga atgatatctt ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt
     2221 ctttctgtaa gagttatcta tttgttttct tttttctcta tacactggat aagtcaggca
     2281 cagccaagaa agactgagta agtagccgcc gatgatatca gtaggaaaat gaacgcccag
     2341 ataaatgcgg cttatcccga cacttactat aataaacatc atcagcaatt gaagcaatgt
     2401 gcgtaaggtt cttttttgaa caaaagctgg aagcagaaat agtaaagaac cgtaaagaat
     2461 catactgcct gctgcatgtc cactagggaa actgtaactt gtttcaacta ctaagtgcgc
     2521 caatgaagga cgctctcgca tgaaaaatag tttgatcacc gggttgatga ttccagcaat
     2581 tacaataaat ccagaaacga accaaaatgt ttctgccctt tttttcttat accacatcca
     2641 taaagccgca caagcaaata aaatgatgac ggtaagagga ttaccgaatt tagtcataaa
     2701 gagttggtag ctgttcagat caggataaga agctctgaca atgcctgtaa accactgatc
     2761 gaaaccagaa agccacgaca gatggaactt tacgatccag cccaaaatga aaaacaggac
     2821 aagaaagaag ctaccagcca cctgaaatga tgttttatat ttcatacaat tcctcctttg
     2881 aagcaatgac ttattatagc atttttggtc cagcgaaaaa aacggtaaag agataaaacc
     2941 aatgtatgat tcctctttga ataaccaata ttcttataaa aaaaataact gtaatcggaa
     3001 agacattttt tggtaatgca aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat
     3061 ccttttaaac aagaacgtag acgtaggaag aacttgaaaa tgaacagaag ttgttcatta
     3121 cggtaaatta caatatgatt acaaaaaaca acaaagctct taaataactt ttaataaata
     3181 ttaagagaag ttataattat taataataga taaattgaag ttggaggata gaagatggaa
     3241 aacattgcaa gaaaagaacg gagacgttta gaacagacca aacgttttcg aaaagcaaaa
     3301 cgaggggcag caatcgtggg aactgcgatg gtaggctgtt ctgtcgctgc cccattagta
     3361 cagccggttc ctgttcaagc ggatgaaaca cctgttcagt tcggcgcaag aatcaataca
     3421 gcggcattta tcacagaaat tgctacttat gctcaaccaa tcgctcaagc caacgatcta
     3481 tatgcatcag tgatgatcgc acaagcagtt gtagaaagcg gttggggcag tagtgcgtta
     3541 tctcaagcac cttattataa tctttttggg ataaaaggca gctatcaagg acaaaccgtc
     3601 tacatggata cgcttgaatt tttaaataat caatgggtaa ataaaaagga gcctttccga
     3661 caatatcctt cttttgctga atctttcaat gacaatgcct atgtacttag aaatacttca
     3721 tttggcaacg gttattacta tgccggtact tggaagagca acacgaaatc ctatacagat
     3781 gcgacagctt gtttgacagg gcgttatgcg actgatcctg gttacgcagg caaattaaat
     3841 aatatcatcg caacttacgg attgacccaa tatgatacac cagctagcgg aaacgcaggc
     3901 ggtggagcga ctacaggtgg caataatact ggaagtgcag gtagtactgg cggttcaagc
     3961 aattctggta atacaaactc aggttcagca acttcgacca cttatactgt ccaatctggt
     4021 gattctgttt ggggaatctc aaataaattc ggtatcacaa tggatcaatt gatccaatgg
     4081 aacaacatcc aaaacaattt catttatcca ggacaaaaac tgacaatcaa aggtagccag
     4141 gaaaatggct cttcgacgaa taattccggt aacaatacca actcttcagg aaatgctggt
     4201 acatcaaacg gaggacaaac aactggtgcg aaatacactg ttcaatctgg tgattctgtt
     4261 tggaagatct caaatgatca cggaattacg atggctcaat tgattgaatg gaacaatatc
     4321 aagaacaatt tcgtttatcc aggacaacag ctaatcgtat caaatggcgg ttcttctaat
     4381 gctggagcaa atactggctc aaatactggg aatacaagtg gctctagttc gtccagctca
     4441 agttcttcta atacagcttc aggaacgaaa tatacagtta aagccggaga ctctgtttgg
     4501 agcgtctcaa ataaatacgg aattacgatg gctcagctaa tccaatggaa caatatccaa
     4561 aataacttta tctacccagg acaacaactg actgtttcaa acagtggatc aacaaacagc
     4621 acgtcaggta atacaggttc gtcaaccaat acaaataaca gcagttcagt taatactggc
     4681 agcagttcgt ctcaaggcac ttataccgtg aaagcaggtg aatctgtttg gagcgtggca
     4741 aataaaaacg gcatcacgat ggatcagtta atcgaatgga ataatatcaa aaacaacttc
     4801 atttatccag gacagcaatt gatcgttaaa ggtggaactt cggcaaatac aaatacaggt
     4861 tcaactactt ctgcagcgaa accaaataca ccgaatacgt ctgctacgac aagtacatca
     4921 tccactggta atacgatgta taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac
     4981 aaacatcata tcacgatgga tcagttaatc gaatggaata atatcaaaaa taatttcatc
     5041 tatccaggac agcaagtgat tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca
     5101 gttactggtt cgaaaaccta tactgtgaaa tctggagaat ctgtttggag tgttgctgac
     5161 agtcatggaa ttacaatggc tcaactaatc gaatggaaca atattaagaa caactttatt
     5221 tatccaggtc aaacgttgat tgtagctaaa taaaatagct gttcgaagaa ataacgattg
     5281 aattccacaa aaatatttgt tactataagg ctacttaaat atgacaacac ttgaaaagga
     5341 agtagtaaaa agactgaagt ttcagagagc gcatggctgg tgagaatgtg cacggatgct
     5401 tttgaactcg cctttgagtt gcccttgctg aatacccgag taagcaagcg gcggtgacgg
     5461 ccgttacaac gttcgaggcc ttttcactaa ggtaaaatta ggtggtaccg cgtagattac
     5521 gtcctataaa aagagcaatc tttttatagg atttttttat ttcttttgtg aagtttgaga
     5581 aaacaaagca aaaggaaata aaaataaaca gattctgccg gttttttcgt tatatttata
     5641 atagaaacaa gtgttttcaa tagaacagga ggacagcaaa tgagttacaa tcataaagaa
     5701 attgagaaaa aatggcaaaa atactgggca aaaacgaata ctttcaacac ccatgatgat
     5761 cctgaaaagc caaaatttta cgcactggat atgtttccct atccatccgg tcaagggcta
     5821 catgtagggc atcctgaagg ttatacggca acggatatcc tttcacgtgt gaaaagaagt
     5881 caaggataca acgtgctgca tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa
     5941 tatgcgctag atacaggaaa cgatccagct gagtttaccc aaaaaaatat cgaaacattc
     6001 cgacgccaaa tcaattcttt aggatttagt tatgactgga atcgcgaagt gaatacaact
     6061 gatccggagt attataaatg gacacaatgg atctttacga agctttatga aaaaggtcta
     6121 gcttatgaag cagaagtagc ggtaaactgg gtacctgaat taggaacagt tatttcaaat
     6181 gaagaagtga tcgatgggaa aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg
     6241 cgccaatgga tgctgaaaat cactgcttat gctgatcgct tattggatga tctagaatta
     6301 gttgattggc cagaaagcat taaagaaatg caaagaaatt ggatcggtcg ttcagtagga
     6361 gcaaatattg aatttaaagt ggctggtacg gataaatcct ataccgtatt tacgacacga
     6421 ccagatactc tattcggtgc aacttactcg gttcttgcac ctgaactaga tcttgttaga
     6481 gaaattacga cccctgaaca aaaagctgct gtagaagctt atatcgaaga gacagctaaa
     6541 aaatcagatc taaagagaac ggatcttgca aaagaaaaaa caggagtatt tactggtgct
     6601 tatgcaatca atccagttaa tggcaaggaa atcccaatct ggatcgccga ttatgtatta
     6661 tcttcatacg gcacaggagc catcatggct gttccagcac atgatgaacg tgactttgaa
     6721 ttcgctcaaa ctttcggact agaaattctt ccagttatcg aaggtggaga tgttgaaaaa
     6781 gctgcttaca cagaagatgg tacgcacatc aactctgaat ttctagacgg catgaacaaa
     6841 caagaagcaa tcgataaaat gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa
     6901 gtcagctatc gcttacgtga ctggctattc tcccgtcaac gttactgggg tgaaccaatc
     6961 ccaatcatcc attgggagga cggaacagtc acagctgtcc cagaagaaga gctaccatta
     7021 cggttgccta agacaaaaaa catcaagcca agcggtactg gagaatctcc attagcaaat
     7081 atcgaggagt gggtcaatgt cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg
     7141 aatacaatgc cacaatgggc aggaagctca tggtattacc tgcgttatat cgaccctcat
     7201 aacaaaaaag aactagcaga ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac
     7261 attggtggag cagaacatgc ggtacttcat ttactatatg cacgattctg gcataaattc
     7321 ttatacgata tcggtgttgt tcctacaaaa gaaccattcc aaaaactata caatcaagga
     7381 atgatcttag gcgaaaacaa cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct
     7441 gatgatgtgg tcgaaactta cggtgcagat acactgcgca tgtacgaaat gttcatgggg
     7501 ccattagatg cttcgattgc ttggagcgaa aatggcttag aaggaagccg taaattcttg
     7561 gatcgtgtat ggcgtttgat cgttgatgag aacaataaaa tgcgtgaccg tatcactaca
     7621 ttgaatgatg gcaagcttga taaagtgtat catcaaacag tgaaaaaagt gacagaagac
     7681 tatgaaaatc ttcatttcaa tacagcaatc tcccaattga tggtctttat caatgaagcc
     7741 tataaagtgg acgctctgcc ttatgaatat atcgaaggat ttgttcaact actcgcaccg
     7801 attgctcctc acatgggtga agaactatgg gcaatccttg gaaatgatgg tggtatttct
     7861 tacgcaccat ggccaacata cgatgaagca gcacttgttg aagacgaagt agaagttgtt
     7921 ttccaagtaa acggtaaagt tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat
     7981 gagctagaaa aagcagcatt agccaatgaa atagtgcaag aatatattga aggcaaaaca
     8041 gtcagaaaag ttattgcggt accaaacaag ttagtcaata tcgttgctaa ttaattggct
     8101 ctttgtcaat aaggactgat gagttgtgca aaatcaaatc tgggccagaa tgaacttcat
     8161 tctgtctcag attttttgtt atttcaaatt gattaattga ttgatcaaaa aggttttgat
     8221 tttcatgttc tcaaacacgt tcaatgtctt tttcttctga atggtcttgt aagagatctc
     8281 tgtaatcatc aaatttttgt ctattaatct tagtattttt tgatgtataa actacctgaa
     8341 attgagagta cataccaaat tcataaattt tttattagtg ggactagtga ttgtatggat
     8401 tttgacgcat aatgaatgaa gtatgagaaa aggagagaca aatggatcaa gaactattta
     8461 ttatcgaagt attatcagct aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa
     8521 gaataatgtt caagctttta agcttagtat ttttctctgg tttgcttttt ttcggagctg
     8581 tatcgttcct atatcgccat atgagtgcag tagtccgttc agctgaaggg atgcaaaaat
     8641 taaataattt gtatcacttt tcgtggggag ttggcggaat aacaattgtt gtgcttattg
     8701 tttttcttct acttatttgg ctccgttacc atcaattgtc gcgtcatttc gatcaatggc
     8761 ttagtcaaag agcacaacag tttttacata ataaaccaac ttctcaagat gatgactatc
     8821 tttattttga gaagacttcg acgagtcaag cttttcggat acaaaaaagt gattgccagt
     8881 tattgctgag atcacttgat gatttaccta tctacttagg cgaaagcaaa tcaaaattcg
     8941 gaatgaacac attccatatt tttttactag aaaacgaacc cagatgggaa aaaagccagt
     9001 cttcgcctgg tggatacctg acaactaaga tgagtacatt agtcactggt tttttcctca
     9061 ttcttttagc tggaacaatc tattatggga gatacactca gccaaaaatg tcttctcata
     9121 taaatgctgc ttctcacatg acaatgaact caactaacga tacgcatata caaaagacaa
     9181 atgaagggtt gttagctgat cctaagcaaa aggtaaattt gacaacacaa aagataaatg
     9241 atctcaaact tgatacagag acgcactctc tctatatgac aacagatagt ggtgctaatt
     9301 gggtatttgt gccgattgat tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg
     9361 ggctgattcc tgaaggttat tggatggaca acacatatga tatttccgcc gatttctcat
     9421 ggtttatcta ttcttctggg gatgatgtac gtctgctgac ttcaagagat aatggtaaaa
     9481 catggcaaac gtctattatc acccaatcag ttcagccgat acgttatcga aaagcggtat
     9541 tttatggcaa cgatggtcta tttatctgta catcagccag tgggatgtct gcagaaactt
     9601 tgtacattta cactactatg gatggcggaa tcacttggat aaaaagtgga agtacaatga
     9661 tcaatcagcc agtacaaaat gtcagcttcg ttacaacttc tttaggtttt gtttcgacac
     9721 gtgaaagttt gttttataca aacaacggag gacgctcatt caaagaatca gttctgtcga
     9781 ttccggaaaa ctatcagaca ggggggatag atctttttca atcaccaaat gaagttactc
     9841 aaataagcgc gaatacgtta gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg
     9901 cagggaaaat gtttgcctgc ctcttccgtt caactgataa cggggaaaca tggcaattcg
     9961 ttcagcaact atcgcaagta gaacaaggag attaaagaag agagattgtg agcctgacgt
    10021 tcagcaccga gtcacaagaa acaaattcaa aaaacagctt ctcatatttg ttgaatagtg
    10081 taactcgtga ttcggagttg acaagagcaa caagaagcga ttttgaagta tagcttctcg
    10141 tattttacaa gatcgtgtct tattgccgat aggttgtctc ttgaacaccg tttattcgta
    10201 aaaagggatt gtgacaagtc ttttgtcact acttaccgaa cgaaactgaa ggagctatcc
    10261 aaaaagtatc attagtatta ttctttgtgt ttctagtggc accactagta catttaaaaa
    10321 agctatcgat catggactaa tcatacatga tcgatagctt tttagataat gtttgttacg
    10381 ctatctgaaa ttgactatta taaagactgg cataaagcgt tggtgattgg agtagttcag
    10441 aatgtgttcc tttttcgacg atatcgccat tcttcattac aaggatcatg tctgcatttt
    10501 caatcgttga caaacggtgt gcaatcacaa aacttgtcct atcttttgtc acagcttcca
    10561 ttgcttgctg aatcaatgct tctgttcgtg tatctacgct agaagttgct tcatctaaaa
    10621 taacgacagg aggattcgct agaatgactc gagcaattgt caataattgt tgctgtcctt
    10681 gagataacgt gccgttttcg ctagaaatga tcgtatcata tccgtctggc agtgttcgaa
    10741 taaagtgatc acattgtgcg atttcagccg cttcgataat ttgttctctt gtggcatcca
    10801 ttttcccgta ggcgatatta tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa
    10861 ccatcccaaa agaacgacgt agttctccac gtgaaagctt ggtaatatca agaccgtcga
    10921 attcgatctt tcctttattc agttcataaa aacgcatcag cagattgact aaagtcgttt
    10981 ttcctgcacc agttggacca acgatcgcta ccgtttgttt tggctttact gaaaaatcga
    11041 cttgattcat caatatttta tctgatgtgt aaccaaactg tacatttttg aaagctattt
    11101 ttcctttcgg ttgttctaaa agtcttgcat ctggaaggtc tggaatttca tctgtttcat
    11161 ctaatatctc aaatatccta tcgattgctg ccattgcgga ttggatcgag ttgatcacat
    11221 aagaagcggt agaaattggt tcagaaatct gattgatgta ctgaagataa gcttgtaaga
    11281 agccgatcgt cacaccacct gatagaacaa gcatagcacc gaataccgca cttatgatga
    11341 aggcgagttg attaatgaaa cgaatggccg gatagatagc acagttcata aattgagcac
    11401 gtttaaaagc gttgtattgc tttttattta cttcgtgtat agcagcttct gcatgagctt
    11461 gctgattgaa tgttttgacc actagattac cagctaagat ttcttccatt cgattattta
    11521 aaacacccag ttcatactga ttctgttcag aaatctgttt gtttttgttc gcaatcttag
    11581 ttgtaacaat tgaactgcca acgatcagca ataagacgag caaagtaagc ttgctttcga
    11641 tataaaacag catcagacca gcagcagcta ttgttacgac agaagtaaaa aattgattga
    11701 tacctgtcaa taacacttga gaaagttgat tgagaccggt agttgtccga ctaatgatgt
    11761 cacctacttg atgattgtca aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt
    11821 cttttcgcac cctcaatgtg atcttttcgc ttaacgctgc cattgtccgt tcctgaataa
    11881 atgaagtgat gctgcttaag actgaacagc cgatcagcaa taagacaggg aacaacaatg
    11941 cttgtttgat atcactgaaa gaaagctgtc caatacctgt ttttcttatt tctgataaga
    12001 gatcatcgat cccgattccc atgatcaagg gcatggctac gactaatagg ttcccgaata
    12061 aactgcatag aaacaatccg taaaaatatt tatgttcagg tttgatcatt ttccaaaaac
    12121 gtatcaaagc acgcgtgttc aacgtttttt tcttttccat cgtatccctt ctttctatat
    12181 tccttgtgat tttgcaaact cctgataagt ttggcaatta gacagtagtt cttcatgagt
    12241 accttgtcca acgatttttc cttcatctaa aacaatgatg ttatctgctt gttggattgt
    12301 actgaggcgt tgagcaacaa tcacgagagt tttctccgac atgtgtttat gaagggctgc
    12361 tcttaatgct gcatctgttt tatagtctaa agcggagaaa ctatcatcga atacatagat
    12421 ctctgctggt ttgatcaacg ctcgagcaat acatatccgc tgtttctggc cccctgaata
    12481 attagaaccg ccttgagcaa caaagctgtc caatccgtca ggaagggtgt ccactagttc
    12541 ttctaattga gcgatcttga tcgctttaga caactcttct gttgaggcat attcatttcc
    12601 catctgcaag ttgcttaaaa ttgtcccgct gaataagaaa gccttctgag ggacataact
    12661 gatgtgggag cggatctgtt cttgagtcat ttgagtaaga ggaataccac caagggttac
    12721 ttttcctatc gtagggtcac taagtctcaa gagaagtttc gccaaactac tttttccaga
    12781 tccggtcccg ccaacgatcg ctgttgtttt tcctttaggt attgtgaaat caatatcttc
    12841 tagaactgga tcagagattt cagtatattt aaatgtgaca tgttcaaaag cagctgcatt
    12901 tgtctgatca aaggttggtt cagctactgt tttgatcgtc tcttcctcaa tttcgatctc
    12961 tgcttgcagt atttcttcta tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa
    13021 taccatagct gcgatcatca agtaagcaag tgtcagcata gagtattcga ctacagcagt
    13081 cacgacaccg atttgtaatg ctccttctgc tacaagattt ccgccaaacc acaaaataga
    13141 agcataaacg attcccatga gcaaaaaagc aattggagtg acccaagcaa atattcggtt
    13201 cactttgatc atttgattag catagtcagc aaagctctta ttcgttcgct tttcttcata
    13261 tgattgatta tcaaaggcac ggatcatatt aatcccagta aagaattctc tcaatgtgat
    13321 ggtgatccga tccatttttg gctggatact caaagataaa ggtgcccctc gtttcatcag
    13381 tcgataaaca atccatacat aaaaaacaac agagatcaat gtaacccatg ttagtaatag
    13441 agaataacga aacatcatga gaatggtgaa aacgcaaata accggtgctg gtaagatcat
    13501 ttggcaaaat aaaacaatca tttgctggac attatcgaca tcattcgtca ttcttgtcaa
    13561 aagagatcca gaactaaaac gactgatatc cttcattgac agctgttgga atttacggaa
    13621 atatttctcc cgcgtttgga atccaaacct agcggaagcc aatgctgaaa agtaactaga
    13681 ggcaactgca gcgattgttc ctagaaacgc tacaagcagc atttcaactc caattttttt
    13741 tataactgag gcttcgccac ttgctatacc tgtgtcgatc agctttgcta ctaattttgg
    13801 tacacctaat gtaccggcga tttctaaaca gatcatcaca aaggtaagga gagcgagtcc
    13861 tttgtttttc tgtaagaatg tttttaagat agacatatta ttcctccttt tttcctgata
    13921 cttttcgatt ctataccttc aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa
    13981 aagaggtgag aaaaaatgaa aaaaatgtat acgattggcc aagtatcgga actatttgat
    14041 ttgccaaaat caacgatccg ctattgggat gaacaaggat taatccgttc aagtagacaa
    14101 gaagagaatg attatcgatt gtttgatatc gatgatattt ttatgcttta tgatatcgtt
    14161 ttttatagaa aattagatat accgatcaaa cagatgaaga atttatatgg aaaaacattg
    14221 acggagttat atgagacact tgatgaaaca gaaagaagaa tccataaaga actggtggtg
    14281 atgaaacaaa aacaaaaaga gataagggag caaaaaaaac agttgaaatt gatgatcgat
    14341 acaaacgagg aagaatttcc tgtagaagag atcccttttg actgtatgat ctcaacggag
    14401 tttgaagata ttgtggaaat caaaaaattt ctacctaact attcaagctt tgggatgatg
    14461 agtatgccag cttccagttc acagacgatg tatggatttt ttatcgaccc tagtgaagta
    14521 catttattta ctcaagacgt gatttgggag aaaaaagata cggctgttta tcgtcgattc
    14581 ttattgaaat ccgagatgaa ccatgcggaa cgtaataata ttttagaaat cagagaaaga
    14641 atgtatgaaa aaggatggaa aacaggagaa gtgatcggcc agtatctatt gacgaatacg
    14701 gatgaaaaca atatcagaac tgaatattat catgcatgga tagaaatgaa aaaataggaa
    14761 atatgtgttt aagataactc gaaaatgatg gttgttttag ttataaagaa aaattttctg
    14821 gttcgattac gggtttaatg tgggggatta ttagtcgtgg atagatagaa ttttagtttt
    14881 ttagaggaaa tatgtcttta aaaatggaat ggttgcaatt cttagcgata tatacgatgt
    14941 aattgtcctc ttaaaaaagt atggaaaaat aattgtattg ggtgttatgt ataattcatg
    15001 gtgaactgag actaatgaga agaaaaattc atattgttat gagtgagatt gtatcagatg
    15061 agaatgataa attaaatagg tgcataaaag cattacactt aaaatacatt gttgcttaca
    15121 ttattacaat atgttaataa ggcaaaaata aatagatgga ttgaataaac atatttttta
    15181 atcaaatagt ccctactgtg actttcctca gctatcggtg atactaccga tagcattttt
    15241 ttgcttataa aataattata agtatttaga gatttttctt ctaacattct tcaaacaaga
    15301 aaaatcacaa aggttattgt tatttttata tattcttttt aagaatgatt gtttttttat
    15361 agaaatttta aaaaataaaa cacgttattg atggatagtt agtttgttat ttggtaatat
    15421 tatagaagtt gactggtagt gatttatttt tcgtcgtttt ataaaaatgt tattttttgg
    15481 aaaaattcac aaatatgtaa cgaatgtttt tctggaattt gctgtgttaa aaagcagtat
    15541 gactaatata aatactattg tcatttatgc aattttttat ggatagtaat tttagagggg
    15601 aatcaatgaa ggaagaatag aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata
    15661 gcaatacatc ttcaagcttt aaaattcgag agaatcatct tggtagtgga atcaactaca
    15721 acaaaaggat taaaccaatt aatagataga attaccaatg gaaaaataga aaaggtagtt
    15781 attctataca aaagttgttt aattcgcttc ggttatgaac ttagtgaaac cctttgcaat
    15841 atatggtaca gaatatagaa attattgaca ttacagaaaa tgatgaagat ttaatacaga
    15901 ttgtcactat ttgtagatac cttttacaag gaaaccgagc caataaagct aagaagatga
    15961 ttaaggagtt gatggaagac gatacaagtt aagaaagtga aactacgccc aaataaagag
    16021 caaaaagagt aattggacgt ggaaaatgca agaaatgaac catcgattcg gtggacagtt
    16081 tattagtaat gacggtttga gaacatccat gacgagaatg aaggaacatc ctaaatgcgt
    16141 ttagttgaac caagtgccaa ataatatggc caaataagcg atcagttatc gatgaatccc
    16201 aagtattcta aactatatat tcattttgat gctaagcatt gatatatttt tttgggattg
    16261 atgaaggtta accaatacag ggactcactg atgaaattct agagtctgac cttggtatca
    16321 acgatttagt gattgtttct aatggaaaag tataaaagta tataagtaca ttaacaaaat
    16381 aactattagg gttacgctcc agcttgtgca aggcactggc aggaggaatc gtgaaatcat
    16441 aattatggtt aagccacacg gacaactaaa agtcatacaa agctatcgaa tcaatcaaca
    16501 gaagacaatc ggcaatcatt agctttgttt acagcttaca ttgcaacgct tgagttgaac
    16561 cgaaaatacg agggaggtaa cgcagccatt ttgagttggg gaatcttttt agaaaattat
    16621 cgggtacctt gttgtttagt caaaattata agggggagta atagtgagga acaaatttca
    16681 taaactgtta agtatgtttc tagttatgct tattgtgatc aatcaatttg ggaacataac
    16741 ggttctagct gagtctttag cagaacagcc agagcgttcg ctgacgatcg aagttgatca
    16801 agaggaggct gctttgggtg agcagttcca gttgcgagtc agaggaacac cagacctgct
    16861 aaatgcattg gtcattgata agagtgagga tttgattgaa ggggaagtga agcaactcaa
    16921 tgattacgaa cgtctgatta cttattcttc ccagcattca ggtaaatacg aattgaccgc
    16981 aagaagcggt gatattcaat caaatacggt ctcaatagaa atcagtgtac cagaagaaag
    17041 cacaagtgag tcttttaatg taccagaaga aagcacaagt gaatctttta atataccaga
    17101 agaaagctta agtgaatctt ttaatgaagc cagatcatct gagccatcta taacgcagga
    17161 atctcagatt gaaagacaag aggagctctt gaattatgac atcggcatct ctgctgagcg
    17221 ccccactttg ccgagcaggc atacacaaac gttaaaggga tacattcaag gaggcgctgg
    17281 cgatattctt gaagaatatg gggagatggt cgtacgagtt cctagagcat cgattggctt
    17341 aattgagttc attagagtgg aggtcgctga tgattcacct ttaaagccac tggcatttta
    17401 ccaagaaggc acagacgttg tttttccatt tacctttgat tatgaaggat taaatgaagc
    17461 cgctcagtcg tatacattca gtatcacgtt tgaagttgct ttctttcaat caggcggtac
    17521 tgagccaaat aatgactttc aagtgagtct tacttatgct gagcgagcag caaacacgga
    17581 attttcaatt acgcctattc cttcatcaag acccattttt tcgaaattat actatggctc
    17641 tagagaaatc gacagccaag gaaatcttgg gaatctgctg attgacgggc aagacgtgac
    17701 caacagtaat aaatttattt tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt
    17761 ggttaccgat gtactaccgc aaggaacaac tttggcaccg aatccgacta catggagttc
    17821 cactggaaat agagaaccta tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg
    17881 tgaagcaaac gttagaactc attatgtttc cgaggaattt gtggacaaag tatcttttaa
    17941 taaggaaacg aatacacttg ttgtggactt tggtaatcta gcagagggtc aatcgtattt
    18001 tattgaatat gctcttgaag tgaccgatgt cgacttgttc aatgaaggaa gacaagagga
    18061 tgggttttct cggaatcgag cgagtatgac ctacaataat caggtaatga gccgagacta
    18121 ccgcttgaga atccctgaaa gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa
    18181 aaatgttatc aatctcaatg aaaatgaact aacgtatact ctgcatgtct cgatgttttc
    18241 tggaacaaga aatacgcttg ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc
    18301 atttctatca gatattccct ctttcattga tgccgaagtc aataatggaa tagttcgttt
    18361 cagtgcgaat aatacgattg gtggtccgac aagttttacc ctctctttta aggtcaatgt
    18421 agaggcgtat gcggtcggcg atcaaatttt aaactttgca acgcttgtga atagtgacaa
    18481 tcgattgaat acgagtactg taagaacaag gaaaatcgat gggcgtatta aagtaattaa
    18541 aactgatgag aatggccaaa ggctggcagg agcaaaattt gaaattttat caagcgattc
    18601 acaagtgata caagaaggta taacggcaga aaacggagaa tttttaagtg aaccgcttcc
    18661 tgtaggtagc tatcaagtcc gagaaacaga agcaccggaa gggtatcttt tagatgcgac
    18721 ggtacatgat gtttttataa atgaatactc catagaagaa gtgacgttaa ccattgaaaa
    18781 catccttgat acgggtggtg ttgaactaat taaacatgct gaaggcagtg gtgaagtcct
    18841 tcaaggagct gtttttgagt tacagaaccg agaaggagaa acgctacaaa ccggtctaac
    18901 tacaggagaa gatggaaaac tggcaattga cggtttagca ccaggaacat atcagctggt
    18961 agaaacccaa gcgccaatag ggtatgaatt agatgcgaca ccaatcgagt ttgagatcga
    19021 aagaagtcag acagcggtgg tagaattgac gaaagaaaac cgacttacac caggcggtgt
    19081 cgtgttgaca aagatcgatg accaaagcgg ggaaatcctg caaggagctg tttttgagtt
    19141 acagaaccga gaaggagaaa cgctacaaac cggtctaact acaggagaag atggaaaact
    19201 ggcaattgac ggtttagcac caggagcata tcagctggta gaaactcaag cgccaacggg
    19261 gtatgaatta gatgcgacac cgatcgagtt tgagatcgaa agaagtcaga cagcggtggt
    19321 agaattgacg aaagaaaacc gacttacacc aggcggtgtc gtgttgacaa agatcgatga
    19381 ccaaagcggg gaagtcctgc aaggagctgt ttttgagtta caagatgcca atggaaccgt
    19441 tctgcagtca ggtctaacca caggagcaga tggaaaactg gcgattgacg gcttagcacc
    19501 aggagtgtat cagctggtag aaacccaagc gccaacgggg tatgaattgg atgcgacacc
    19561 aatcgagttt gagatcgaaa gaagtcagac agcggtggta gaattgacga aagaaaatcg
    19621 acttacacca ggcggtgtcg tgttgacaaa gatcgatgac caaagcggag aagtcctgca
    19681 aggagctgtt tttgagttac aagatgccaa tggaaccgtt ctgcagtcag gtctaaccac
    19741 aggagcagat ggaaaactgg cgatagatgg cttagcacca ggagcatatc agctggtaga
    19801 aactcaagcg ccaacggggt atgaattgga tgcgacacca gtaactttca agatcgaaaa
    19861 agaacaagaa gtggcgatct ttttaacaaa agagaataga aaagttgctg atagctcaga
    19921 tataagagga aacaatccaa caagcagtgg aaataagcat cttccaaaaa ctggagaaac
    19981 catcattagc cagtttatat tgtcaatatt gggtgtgcta cttgttttca tcagtataaa
    20041 atttagaaag aaacgaaata tatgagctaa aataaaaaat tgaataagca tagtttttaa
    20101 aatcaactag tacccatcaa ttgaatggaa actaccgatt ttagaagggg tatacaaaca
    20161 aatattagta tcggagtaga gaaggttgaa cccaaccagt aaataagtaa agacaatttt
    20221 gtgctgcgtt gagaagtgaa aaataaacct ttgtagtaat actagttaat aaaaaatata
    20281 caaaaacact gcgaaaatca ttcttgagtt tcgcagtgtt ttttgcctaa tgactcaagt
    20341 ttattgggta atactttgat tttctttcta caaataatca gcgatcagtt gtaatttttt
    20401 ctctagtttt aatggctctt tgtcaataag gagaaaaaca agtcgggtct tttgtctcgg
    20461 aaagcttcaa ccaactcgtg aataaaggtt acgcctcctt gaaaggcgtg gaaaaaccaa
    20521 gtaatcgatc aatcaccaat ttcgcatttg gtagaacatg tttggtgagc tgaaagtagg
    20581 tggcgttcat gtctgtaact aagaattcta tttctttagg attagtgcag ctaaggaaat
    20641 agtttgttaa tcgaggaatt ttttgtgtag gtaaattttc tcgattaatt tttctgtctc
    20701 gccatccgcg gaaataaaac tcagtttatc ctcaacggaa acgtgagaac gaaattcaca
    20761 accctcaaca ctcaagacag aattcttttg gaagactttg gtaaatagct tctcaactct
    20821 ttcaatgtgc gaataacggt tgtcaaagat acctaacaac ttttcgaaat aaaaaataac
    20881 gacacttttt cagtcagtaa agaagtgatt ttatgtctga catgatttga aatcgagtgc
    20941 cttggacgga taaaataact ttgagcgttc caatggcttc gacagttttt acaggtatag
    21001 cgctgctttt ttaggcgtat aaccattggc atatgattat attattcaaa gtggacaatg
    21061 gtttccttct tcttgttttt caccaccatt aatttcctat ttccatctac cacagcagaa
    21121 ccacaatttc tacagacaca tggagcaggg aagagaactg cagcaacgat caatgttttt
    21181 ttcttctgta aggtttcgta agagacctct gtaatcatca aatttttgtc tattgatctc
    21241 agaatttttt tgatagaacc attcataaag catatcgttc tctcagttgt ttattttgtg
    21301 gtgatttaat catacaagaa aacgatatgt ttttttataa aaacatgaat aggaaaaata
    21361 gttgacaaga aaatgacaac gccatatgat gttccatagt atttacaacc tgtcatagat
    21421 gactgtaaaa aataaaagta aaaagaaagg acgacaacag gtggattttt atcagtctct
    21481 acaactagat ccgtttattt taaaacaaaa aatacgagaa gcagcttcta aaaaagaaaa
    21541 gtgcatgtat atttgttcat tgtttttacg atcattattc atcgtattgt ttgccatttg
    21601 ttttatcatt ataattacaa cattatttga atcgaagcat aaaccatacg ctgtggtttt
    21661 attctgtatg ttgatgagta ttcgttttgt tgactttggt tataagattt cccattcgat
    21721 cataggttta gcaatcgtca tgttctcact attagtagca ccgtatgtcc agctgatcaa
    21781 atggtcagtt atgggcatgt tgattcattt tattttacta agcagcatat taatggcaac
    21841 cgctagtgat cccaaaatgg ggaatgcttc tttgtacggc ttttcatatc tctttattgt
    21901 ttattcgtta cctaaagatt cgttgaacaa gaatttcttt acacaaacaa gtagcttatt
    21961 atttttgttt ttttgttggt tttcagttct cctttacaga aaacatcgag aaaagaacaa
    22021 agacaaaagc ttgtttaaag agattttttt gaaaggcatg tattcgcagg aaaaaatttg
    22081 gatgctcatc tatgcatttg gcattagtct tttgatcgtt gcaggagagt atgtcccttt
    22141 tcagcgattg atgtgggcag ggtttgcatt ttcttctatt gtatctagct atggattgat
    22201 gtcgattggt ttcaaagaac gagcagtaga ccgaatcatt ggctcattga ttggatgtgt
    22261 tttatttatt ggtatcagcc aatttattcc ttttaactgg gtcggtatat taggtggtct
    22321 agctttaggt gtttgttcta cctatagata taaaacagtt tttaattctt ttggtgcttt
    22381 ggcaatcact gccagtttat tcggggttcc aggagcagta acgatccgca tttttgagaa
    22441 tatccttggg gtatgtttag gaatcatgta tattggggtg actgaaatac ttatccgaaa
    22501 aataagagaa aaacacggtt tgaatcatta gatgagatcg aataaaaaat aaggaaaaag
    22561 tgaagataca catggatgat aaattgctat cattccatgt gtatcttttt acggtaaatc
    22621 agtgaaacgt tcgactttca tttttaattt atacttttta cggagttcgt ttatttgagc
    22681 catcatttcg gatttgtgat gcttttcgat tgcttcttca tctttccaac ggtcgatcag
    22741 taaaagagat tctggatctt ctagtggaaa gtaatagtca tatttctcgt ttccttcttc
    22801 tgcacgaatc gctttgacaa tgtttctttc gaccatttct tctgcaaatt ttctggcttg
    22861 tccatttgta cttgtataca aaatattcac tgtgatactc actaaatccc ctcctgaaaa
    22921 aattctatca ctagtatatc acggtgccag aaattattat tcaagatatg atacacattt
    22981 tttccgcaat gctacggaaa tatatatttt gaatagaaaa cgcttacaag atatgcttgg
    23041 tttataaaaa taaaaggagt gattgattta tgaaaaaagc attgattatt tgtgcggcag
    23101 gaatgtcatc ctcgatgatg gcctcaaaga caacggattt ctttaagcaa aaaagagaag
    23161 aggtactcgt agatgcagtt tcagctacag aaggcgataa tatgattaag aatagtgatt
    23221 ttgatttatt tctgatcagc ccgcaaacga cgatgttttt ggataaattc aaaaaattag
    23281 gagaatctgt tggaaagcct gttgttagta tccctttcca agcgtatgtc ccaatcccta
    23341 caggcatcca gaagttagca gaggtgatcg aagagaacat atgatggaat aggagtggat
    23401 gataatgaaa cagaaggaaa aattgctgtt gagatatttg attgatcaca agaaggagta
    23461 tgtcaccagt caacgtttag cttctgaatt gttattatct gatcggacaa tccgtaatta
    23521 cttgcagcgg atcaaagagt tagtagaaaa aaacggaggt aagatcattg ctaaaccagg
    23581 ttatggctac caactgcata ttttacaaag actgacattt gacttgtttt tgagtcggca
    23641 agaaatagta cctggatatg ctagagaacc gcaaggattt tatgagtccg aagatcgaca
    23701 aaagtatata ttgaaccaat tgcttttgga agatgccatt ctttttatgg atgatttggc
    23761 agaagagctt tatatcagtc gttccagttt gacaaaggac atgcaagaaa tcaaggagcg
    23821 attgattcca tattcactaa aaatcgtttc taagcatggt caagggacat ggatcgaagg
    23881 ggaagaaaga aatcgacggc attttatcat ggatactttt tttggaaacc attatggaaa
    23941 ttcattgaaa gaatatttag ggcaaagccg ttttttccca gacatcagtt ttgaagagct
    24001 agtcattatc attctcgatg aaacaagaga agctaagtta aaagtttcgg attttatcat
    24061 acagaatctg actttacact tggctttggg gatcaaacgg ctccgagaag ggtttgaaat
    24121 caaggaatta ggcatcgaac aagaagtttc taagcgagta gagtatcaag tggcgcagcg
    24181 gatcgttcaa cgtattgagg taatcgcagg tgtatcgttt cccgaagaag aaattgctta
    24241 tttgacatta catctcatgg ctaagtcgaa tcacaaagaa aatcaaaatg atcaggaact
    24301 atcaaaggaa ttagcgaaag ttctgaaaag attgtctcag gaatttcatt tttcattagt
    24361 agaggatctc cagcttttca atggactact cgatcatttg tcaccgatga tgattcgatt
    24421 gagccgaggt attcgcattg agaacccatt gactgaggag ataaagaaag aaaatcccaa
    24481 agtctttgac gcagtaaaaa ggcatttcag caacatgccg gctttgaaaa actataccat
    24541 caatgaagat gaatgggcct atttggcttt gcatctcatg gcggcattgg aaaaagaaag
    24601 agctgcgcat aaactacatg cattgattat ctgtgcaacg ggttacggca gtgcccaact
    24661 attaaagaat cgcgtagtca gtgaattcgg taaaaatatt acagtggtaa gtgtaaaagg
    24721 ctattatgag atcaatgaaa caactttaaa aggaatcgac ctgattattt cttctattga
    24781 tctttcgaca atgtttttca aaattccagt tttacatgtc agtatttttt tgaatgaaga
    24841 tgatgttcgt aaaatacgaa aggtagttgg agaatctata ccctatcatt cctcagatag
    24901 accgatacca gcattttctc tacagcaaaa aaagcagatt tacaccgaac agttgtctga
    24961 aaaatttttc aagacttata cgtacgaacc gaaaaaggaa gaaattcttc atgatttgct
    25021 gcaaaagctg agcataaatg aagaagaagg atatataaga gaaatgcgaa agcagatcca
    25081 gcaacgtgaa aatatgggac aaatcatatt cagtgataca gtggcagtcc cacatcccgc
    25141 tgttccagtt ggaatcgaaa caaaggtagc cgttgcattg attccttctg ggatgcagtg
    25201 ggatcaatat caagaaattc attttgtttt tttagtttcg ccttcttatg tcgaaaatga
    25261 aggaattacg gtcgtgacta aagcaatcgt taggttagtt gaccagctaa aagtacagca
    25321 ggaaatctta gctgaaccta tttttgagaa tttcagcagt aaatttatca aactgatcga
    25381 ataaggagga aaacagatgg ctgaaaaaat aaacagtgaa acactacaag tgacttcttt
    25441 tgatatcatt ttccatagcg gaaatgcacg gacactgata catgaagcat ttcgctcgat
    25501 gcgtaaagag atgtttgaag aagtagaaga gaagttgcga caagcaaatg aagagattct
    25561 tcatgcgcat cagtcacaaa ctcaacttct gaaagaatat gccagcggga caaaaataga
    25621 aatggaaatt atcatggtcc atgcacaaga tcatctgatg acgacgatga cattattgga
    25681 agttgcacag gaaatgaatt atttgtatca aaaaaataaa tagaaaggaa ggatcattgt
    25741 gcaggataca gtaaaaagga aaatcgaatt agaaaaaaag caacttagtt tgaaaaacat
    25801 gtatttcaat cgttatctaa tcgtacgtta cctgactgcc tttttctttt tcatgaacat
    25861 gcaatggatg attctgcttc tatcagctaa aagtttagga agtagtttac cgatgattct
    25921 gattttagct attttgcctg cagtaggaga acaagtaaag ctttatcgaa aacaccaaac
    25981 gaacgttcca tggacaaagt ggtatttttt gttccagagt gtctttaatt tattagtgat
    26041 tccagtgttg tttacctcag gatttacatt cttgtatcct tttatggcaa ataacaatgg
    26101 aggacagtta tttgttttca ttcttgttgt aagcggtatt tttgccagtg catttataca
    26161 gtatcgttta aagaaaattt cactcaacca agaccaacag tatatccgta ttaaacaata
    26221 tgaaaaagcg ttatatttag gaaaggagaa taactaatgt ttgatttttt gcagaaatac
    26281 ctgatgggac ctatggggaa aattgcgcaa cttaaaatcg ttcgggctgt catggcagcg
    26341 gggatggctt ctattccttt cacgattgta ggctcgatgt ttttggtttt aaatattttg
    26401 ccattaccgt tcccattttt agaaggattt tttaatgcaa cttttttcaa agttagtgac
    26461 ttgtacatga tcgtcaatac aatgacgatg gggattttat ctatttactt tgccattgtg
    26521 tttgcttatg aattaacgtc aattgaacga gatgaacaaa aattaaatgt taatccgctc
    26581 acgggggcat tactatctgt ttttgcattc tttatgtgta tccctgaatt gatcatgact
    26641 gatggtaaaa tcagcttgat tgcttctatc aatgagacag aaactgtagt aaatggtatt
    26701 aggatgggct cttttgtcga acgtttagga acttcaggaa tctttacagc tattattatg
    26761 tcgattattg cggtcgaatt gtattgtatg tgtgtcaaga gaaattgggt cataaaaatg
    26821 ccagatgttg ttccgccagg agtttctcgt tctttcactg ctctgatccc aacatttgtc
    26881 attgcgtttg tggtgatgat catcaatgga ttattagttg cactaggaac ggatatcttc
    26941 aaaatgattg ctatcccatt tgggtttgtt acgcagttga cgaatacatg gcttggtgtc
    27001 atggtgattt atttcttgat ccatgcattg tggatcgtag ggatccatgg agcgaatatc
    27061 atcacttctt tcttgacccc gatcgtatta gctaatatgg cagctaacgc acaaggcgca
    27121 aattatccgt tggcaggaga attcaacaat tcttatgtaa ccgttggtgg atcaggtgcg
    27181 actttaggat tgatcatctt tattgcattc ctcgcgaaat ctgatcagct gaaagtgtta
    27241 ggaaaagcct cacttgtacc aggattattc aatatcaatg aaccaatcat tttcgggatg
    27301 ccaatcgtct acaatcccta tttagcggtt ccgtttttct tagctccgat ggcttctgct
    27361 acactcgctt attttgccat tcggctggaa atcgtcaaac cgatgcttgc acagatgccg
    27421 tggccttcac cagtcgggat cggagcattt gttggtagtg gtggtgattg gaaagctgct
    27481 gtgttagcag tcatttgtgc aatcatcgca ttcattatct ggttcccatt tatcaaattc
    27541 tatgataata aattattaaa tgaagaaaga gaaaaggcag cagaattagc tagtgaaaag
    27601 ttaaaatgat ttctaagaaa aaaggaactg tgacaaaaaa tcacagtccc tttttttctt
    27661 agaaatggga aaccactagt atataaatac cattattctt caatcaaccg cttcacgatt
    27721 gcataatccc cttcataagg ttcatctaca ccattgattt tttgataggg atccacgcct
    27781 tttcccgcaa taacgactga atcttgcgga gcgctttctg ctaaagcgcg tcgaatcgct
    27841 tcaggacgat ccatttcata atgaacgatc aaatctggat tagtaattgc ttcattgatt
    27901 tcttccgcga tttttttcgg atcttcaaat gctgggtcat ctgccgtcaa aaaggcgatg
    27961 tcagctgttt ctgatagcac ttttccaaaa tcatgtcgac gggagattgc tttgtttcct
    28021 gggctgccta atacaacaat tacacggcca tctggatgtt catttttggc aaatttcagc
    28081 aatgttttca agcttagata attatgtgca tagtccacat agacatgcgc tccattggat
    28141 tggatcaatt gatccattcg gcctggtaca cgagcttctt ttaatccgtc ttgtgcatct
    28201 tgactggagg cacctgttag tgctgcggca atgatcgcac tggctgcatt ttcttggttg
    28261 aatcctccag ccagcaaaat atcataagca gtatcgcaga tagctaagcg atcttcatga
    28321 gaagataaag taaaaccgtg agtaccttta tctgaacgga tcacttgata attgctttct
    28381 tccgaacgac cataagtgat cgttggaatc tggaatagat cggctgtttc cctaagtaaa
    28441 tcaaaatagt cactttcatg atttaaaaca actgtttttg aattcaagat caattgtcgc
    28501 ttgcaataga aatagtcatc aaatgtcgga tgttcgatcg gactgatatg gtcaggcgag
    28561 atattcaaga agattccgac atctaaagtc aagccgtata cgcgttgtgt tttgtaagct
    28621 tgtgaagaaa cttccatcac tagatgagtc ataccatttt caactgcttc tgacatcata
    28681 cgatataaat ctaatgactc aggcgttgtc aaatgggatt tgaaatatgt tttgccatct
    28741 aacgttgtat tcatagtaga aagcaatgct gtttttttat ttgttgtgtg atccaagatt
    28801 gcttttgtaa agtacgcagc agttgtttta ccttttgttc cagtgaatcc gatcactttc
    28861 aatttatttt gcggatagtc gtaaaaagcc atagcaagaa gcgccattgc tttacggata
    28921 tccgttacga tgattcctac tgctgaattt tcgtatggtt gttctgatac ataatattga
    28981 ataccctgtt cgattgctga atccaaatat tcttctttga aattcattcc tttacagaaa
    29041 aacaaagtag aagcatcagc ttttcttgaa tcataactta actgaagaaa ttcagctttt
    29101 tctggtacat caagatgcca gcctcttgtt gaaacaaatt cttttaatag attttctttt
    29161 aatagtaaat gacggatgtc ttctaaagag aagctcattg attgatccct cgctttattc
    29221 aatttcttgg tttattatag cacactcttt ttaatatatc gtttgtttcc gctatgacaa
    29281 aatccagctt tattcaaatt ttcttgacac aggggacaag cggatgagta ttttcataaa
    29341 caatgaactg actgtaatag ataggagaac gaatagtgtc tccaacaatc caaaaacata
    29401 ataattcaaa aagacgataa gactattggt aagtaaaagt ccttgcggca ttggaagccg
    29461 aaaaaatttg tggaaaataa tcggcggtat cgttataccg ccatttgagg ccccgactgt
    29521 gtacaatgtg taaaccccaa gactgaacaa aagacttcct gaaatcagtg aaaagaaatg
    29581 tgaggagatc atttccactt ttggaacaat ctctaaaaca acaggaaaaa gcagacttcc
    29641 cgctacagtc ttaaaaaata ctgattttcc taataggaca gcggctagaa gcagcatcat
    29701 aagattgatg ctccaaacag taactgagcg ttggatagaa aaggccgttt caactaaatg
    29761 tccgatggcg cccactccgg cggaagctat atcgtgaggg agcaagaacc aattcaatcc
    29821 aactgtgata aagaaaagac ctaaaaatat aacgggtagt ttttttattt cttgtgtcat
    29881 tgatatctcc tataaatgtt agccataaaa ggctgtatat ttctttactt taccaaaaaa
    29941 acgaaaccga attcaaaaaa acaattgttc ttataaaagt tatataaaat cgattgccag
    30001 tttctgtttt accaattaaa atgatagtaa tgttttgtag aaagttggaa tctgaccgat
    30061 ggataatcaa agaaaaccag cattagatca attaacaccg caagaaaaaa tggcacgagg
    30121 ctctgcttgg atgaccgcca gtaatatcat ttcaagattg ctaggcgctg tctatatcat
    30181 tccctggtat gcttggatgg gcgaaaatgc taaggctgcc aatggattat tcaatatggg
    30241 atacaatata tatgcattgt ttctactgat ctctacagca ggaatacctg ctgcgattgc
    30301 aaaacaaact gcacgctata attcattgaa tgaatatggc acttcacgac gattatttat
    30361 ccgtgcatta cagatgatgg gcgggttagg cctgttgttt gctttgttca tgtacatcgc
    30421 ctctccttgg ctagctcatg cttctggtgg cggagaagaa ctgattccaa cgatgcgttc
    30481 tttgagtatc gctgtattgg tttttccttg tatgagtgtg atacgagggt atttgcaagg
    30541 caatcaagaa atgatgcctt atgcgctgtc gcagattgta gaacaggtgg cacgtatttt
    30601 ttatatgcta ttggctacat tcatcatcat gaaagtcatg gatggtgact atgtgacggc
    30661 ggtcacgcaa tcaactttcg cggcatttat cggtatgcta gcaagtattc ttgtcctttt
    30721 gtattttttg aagaaacatc aagcgtatac atcggcgttt attcactaca gcgaaaacaa
    30781 aataaatatt gcaacgaagg aattgttgtt agataccgtt aaagaagcga ttccgtttat
    30841 tattgtgggt tcaggggtaa cgatcttcaa attagtcgat cagttcactt ttatgaagat
    30901 catgtctgca tccaccgagt attccaatgc acagttactg gatctttttt cgatttttag
    30961 tgccaatcca gataagttga cgatggtagt tattgcattg gcaacatcga tcgcgtctac
    31021 tgggttaccg ttgattacag aagctgttac gttgaaagat cgagtaggtc tggcaaaatt
    31081 gacaagcgga aatttacagt tgttttcatt ttttatgttt ccggcgacat ttggtgttat
    31141 gttacttgcc tatccgctaa atacattatt ttatacaccg gattctctag gaagtaatgt
    31201 actgatccaa gcaagttttg taggactctt cctaggacta tatatgctag tttccaacat
    31261 gctgcaagga atgtttgaaa acaaggcggc aattaattat ttggttattg ggtttctagt
    31321 gaaactcctt cttcaatacc cagccattcg tttatttgaa gtatacggcc ctttattggc
    31381 gacaatgatc ggatttgctg tgtcttgtac cttgatctta aagagaatcc atgaagtagc
    31441 ccgctttaaa ccaagttttg tctggcgcag gacattgctg atctttattc ttaccttgat
    31501 tatgcttttg gcttcgtgga tcacaaagat gatttttggt gcttttttaa acgaagatag
    31561 caaattccag tctctattgt tgattcttgt agtagcaggt ataggggctt tagtgtatgg
    31621 ttatttagct ttgaaaatcc gcttggcaga aagactacta ggaccaggaa tagcgcgttt
    31681 acgacataaa ttaaaaataa aataagtgaa tcagaagagc tagattgatg ctcttttgaa
    31741 atgggggaaa cgcatgcgtt tagataagtt tttagcagat atgggacttg gcagccgcaa
    31801 agaagtcaaa ggattaatca aaaaaggaac gatcgaagtc aatggacaag tggtgaaaag
    31861 cgataaagcg caagtaaatg aattcgaaga tgcggttact tatttaggag aaatcattac
    31921 gtatcaaaaa gatttttact acattttgca taaacctgca ggagtcattt ctgctactca
    31981 ggataatcat gatcaaaccg taatcgattt attggaagac caggattatc gggaagatct
    32041 ttttccagtc ggccgattag ataaagacac agagggcctc ttgattttaa cgaatgacgg
    32101 ggtgtttgcc catcagttac tatcacctaa aaaacatgtg gagaaagaat atctagctga
    32161 ggttagcggt ataatgacag aagaagatat ccgtttattt gcagaaggac tgattattga
    32221 tggcgatgag cagacattgc cggcaaaact ccaaattgat caagtagatc aagagacaga
    32281 aacttcaatt atccgtctga ttttgcatga aggaaagttt caccaagtta agcgaatggt
    32341 caaagcagta ggaaaagaag tcttatattt aaaaagaatc cgtatgggaa aattttggct
    32401 gcctaaagat ttgaataaag ggcaatatcg acccatgacc aaagaagagc tagaacaagt
    32461 gagagaaaaa taattgaatg gatagctaaa agaataagca gagtagttgt agcgccatct
    32521 gaaaagcaga cttgtccgtc tgactttcgg atggcttttt tgtgaaattt cggcttattt
    32581 ccgtagaagc tacttctgga acaccgtttg ttcggaataa gggactgtga caagactttt
    32641 gttacagtcc ccattgttcc ttgtgactca gagttagata gtaaggacac gatctcccta
    32701 atactcaggc caaaacgctg aattcacaac ctctgataaa cggtgttcaa tcagctgacc
    32761 ctcggtatta gctcaaaaaa cggcaaaata tgaggaacta ttttcccgtt tttttctcta
    32821 atactcaggc caaaacgctg atttcacaac ctctcctata ctttgtttca ttttccctat
    32881 actttgttta tttattctca ttattgaaag cgctataatt acgttgcgaa tgaatcgaaa
    32941 ggggttttta gatgaaaaaa cgaaaggggt tcaatcattt ttttatgaat ctatggcgtt
    33001 ataaagcact tgtcctgatg gctgtaccgg gtatgctttg gatgattttt ttcttttaca
    33061 tacctgtaat ggcgaatgtc gtagcattca aaaattttca tatttcagca gatgggttct
    33121 tggctagctt gagggaaagt ccttgggtgg gttgggaaaa tttcaaattt ttattttctt
    33181 cggatcaagc atttctcatc actaaaaata caattttata taatgtagca tttatccttt
    33241 tgaatctgct gatctcagtc gtatttgcaa tcattatgag cgagttgaga aataaacgac
    33301 tcgttaaagt ttatcaaacg atgtccttac taccttattt tctatcttgg gtcattatca
    33361 gttactttgt ttacgctttt ttaagtcctg acaaaggaat attcaatcag tggatcactg
    33421 gacatggagg agaagctgtc aattggtata atgaaccgaa atattggccg tttatcttgg
    33481 tgttcattgg tacatggaaa gggattggtt ataacagcat tatttacttt gcatctgtta
    33541 tgggaatcga tccgacttat tatgaagctg ctatggttga tggagctagc aaatggcagc
    33601 agatcaaaca tgtgacgata ccacaactta ttccactaat gacgatttta acgattttag
    33661 ccgtaggaaa cattttccga gctgattttg gcctgtttta taacattcct aggaattccg
    33721 gtgcattata tgatgtgact caagttttgg atacgtatat ctacaatggc ctgacatcca
    33781 caggagattt tggtatgacg gctgctgcag gactttacca atcagttgtc ggctttgtct
    33841 tgttgatgat cacaaatacg atcgcccgac gttttgatag cgattctgca ttattctaag
    33901 gaggaaaaaa atgaaaaaaa agaaagtgga gagagtccaa gtccgctctt tcaataagac
    33961 ggctaatttt ttctctaata taatgattgc actctttgct atttcctgta ttttgccatt
    34021 tatatttgtt atcgccatct cttttaccga tgagtccata ttgtcgactc acgggtacag
    34081 tttttggcca gaaaagttca gtatatttgg ttatacgtac ttgttcgacc agatgcagga
    34141 taaaattttc caagcgcttt tcgtcactgt acttgttact gttttaggta cactgatcaa
    34201 tagcacggct acttctttat atgcttatgc gatttcacgg tcaaactttc cattccgtcg
    34261 tttttttacc gttttttgtt tgattacgat gttgttctca ccagggatgg tggccaatta
    34321 tcttgttatg acaaatcttc ttcagttaaa agatacgatt tgggcattga tcttgccaat
    34381 ggctgtcagt cccttcaata ttattgtgat gcgcacattc tttaaacgtt cggtctctga
    34441 ttcgattatt gaatcggcac ggatcgatgg agctagcgaa ctgcgaatat ttgttcagat
    34501 cgttttaccg ttagctgttc caggtattgc gacaatcagt ttatttgccg cattgggtta
    34561 ctggaatgac tggttcaatg ctttactata catccaagat gataagctag ttccactaca
    34621 gtatcttttg atgaaaatcc aaagtaatat tcaatatctg acacaaaatt caggagcagg
    34681 aagccaattg acaggcggat tagcctcagt tccaggagaa tctgccagaa tggcgatcgt
    34741 agtcatctcg acgttgccta ttgcgatcag ctatccattt ttccagaagc attttgtcaa
    34801 aggtctaaca atcggcggag taaaagaata aagaaacaaa aatataaatc ataaaattag
    34861 gaggaagaaa aatgaagtta tggaagaaac tagcctttac aggtgtttca gcgttgttgt
    34921 tgggaacatt agcagcatgc ggaggttctg gttctaaaga ccaggcagaa gctagcaatt
    34981 cggacacttt gcagatgtat caaatcggag ataaaccaga taatttcgat caattgatgg
    35041 aagtagcaaa caaacgaatc gaaaaagaga tcggtgtaaa agtcaatatc aattacattg
    35101 gctggggcga ttacgaaaag aaaatgaacg tcatcatttc ttccggggaa aattatgata
    35161 tcgcctttgc gaacaactat gtttccaatg cccaaaaagg agcctttgct gatttgactg
    35221 aattagcgcc taagtacgct gaaaaagctt ataatgattt agatgaagca tatattaaag
    35281 gaaatctggt caatggaaaa ttatatgctt tcccagtcaa tggaaatgtc tttgctcaac
    35341 aagtacttac ttttaacaaa gctttactag ataaatacga tctttcaatt gatggaatcc
    35401 aatcttacgc tgatgcagaa aaagtcttga aggaattcca tgaaaaagaa ccaaacacgg
    35461 cagcttttgc cattggacaa ggcttcaaag tccaagggaa ctttgattat cctttaggga
    35521 atacgttacc atttgctatt gatctggatg gagatgattc gaaaatcatc aaccaatacg
    35581 ataatgaaac gtatatcgga ctgttacgta caatgcatag ctggtacaaa caaggactca
    35641 ttccaagtga cgctgcaacg agcaataagg attatccact agaaggaaat acttggctaa
    35701 tgcgtggaga aacacaagga ccgtatgatt atggcgatac aattttgaca aatgcagcaa
    35761 aacaagaact tgtctcaaaa gcaattactg ttccactaaa atctacagct caagcacaaa
    35821 tggcaaattt tgtcgtatca aatacttcaa aaaataaaga aaaatcagta gaacttctag
    35881 ggctgcttaa cagtgatcca gaattattga atgggcttgt ttgggggatc gagggagaag
    35941 catgggaaaa agtcgatggt tcagatgata aaatcaaatt gctagatggt tatcagccaa
    36001 atacacatat gtctgcttgg aatacaggaa acaacaagat tctatataca caagagtcta
    36061 ttacggatga catgattgcc aaacgtgatc aatcgattgc tgatgcagaa acttcaccga
    36121 ttttaggatt cagcttcaac actgacagtg tcaaaacaga attaagtaat atttctaatg
    36181 tcatgaatca atatttagat ggattgaata cagggactgt tgatccagat gaaacattac
    36241 cgaaattgaa agacgctttg gacaaggctg gctatgataa ggtattgaaa gaaatgcaaa
    36301 aacaatatga tgagttccga caagaataat aatgaataaa gacaaagggg ttgtgacaaa
    36361 agacgtgtca caacctcttg ttccgaataa acggtgttca aaagccagtt tctcggtatt
    36421 aagctaaata tatgtaaaaa tgtggaaagc catattcaca aaatttttct tactactcga
    36481 aactaagcgg ctatttcaca aactcttggt aaacggtgtt caatcagcta accctcggta
    36541 ttcgctcaca aaacgacaaa atatgaggaa ctatttttcc gtttgtttct ctaataatca
    36601 ggtcaaggtg ctgattgccc aacctttttt tgtataatga gaaagtataa acttaatgtt
    36661 tggaggagaa gatatgttag ggaaggtttt ggaagagtta tttatccgga tctgggtcat
    36721 tataaaacta accttgtatt tctggatata tacatttgcc ggcgggatca tctttggatt
    36781 aggtgcggca tggaaaacag tcaatgaact attttatttg tacggattcg agtataaaga
    36841 aatcacaata aaaagaggct ggaatatata taaaagaaat tttttaagag gcaatctgct
    36901 atttagttta tttttatctg gtactgcttt attgagttac aatttatatt tatctgttca
    36961 aatcaaaggg ctcatttttt tagctattga ttttatttta ctttttgcct tgctctgcct
    37021 atttgctact tatcaataca gtatgatttt agatagtgaa tatacgatct ctgttcctaa
    37081 tctgctaaag ctaagtttta tttcggtttt ttccagcttt tcgtcttttc taaaaataat
    37141 tattggtagc ggtataattt taggcgtgac ttggcaattc aaaggattga tcctgtttgg
    37201 tgtcattggt cttttgacgg tttggaatgg tacgatgaca acacattggc gtgaagaatt
    37261 agataaacag ttggaaagtt atgaataagt ttcggattct attttcaaaa ggaaccttac
    37321 taaatcgtct gttgcggaaa tacgcggtta tcatgatcgg tgtaacgatg actgctaccg
    37381 ttatttttag tatccatact tgggaacaaa atcaaaaaca ggcagaaaac atgacaagtg
    37441 atgccgttca aagcacaagc agaatgctga atgataaaac aaccttgagt aggatcatca
    37501 aaaaccagct tgtagggaat tccgaaaaga tcgaaaatgt gacgacctac ttaacaaaac
    37561 ccatagatca gtatctgatg tatgtatacg aacagcaaaa cagtaccgat gaattagttt
    37621 ctttcccaaa tcaaatcaaa gatctctacg caaattatga agaactaagt gcgatatata
    37681 tcgtgttgaa tcaactaccg gagtattatg aatcttctag agaaaacaaa ggtggggaaa
    37741 taaaaaaggg gacaccaaaa ttactagacg ccttttatat caagttgcct atcacacaag
    37801 gaggagctga gtcaggaagt atttttattg gttttcaaaa gaaagaacta gatatgattt
    37861 tagaaaatct aacgtcattc aatggactct ctttatacat gatttctgga acgacaaatc
    37921 gactatacac attccatgat aataaaatag ctaaagaaac gtttgaaaga caagaaatga
    37981 tcatcacaga gagtctgaag aaaaattcac aattgccgat tgacgatctg gaaaaaaaca
    38041 attttgttca acaccaagaa ctgtctgatg attatcgtat attagctgta ctagataaaa
    38101 aaagtgtaaa aatcgaaacg ggaaaaaatc ttgccccgct gatcattggt atcattattt
    38161 tagacaatat actgcttatc tttctttata aaacattcaa acgatattca cagcaagtat
    38221 ctatcgtcat gcaggccatg gatcaaacag cagcagggaa tttagagacg cggatcgaca
    38281 taacaaacac ggaatatgaa ctgaaggaac tttcaatcgg tatcaacgaa atgttggata
    38341 gcatcaatca gtttgtagaa gacatctaca aattagaaat caaacagcaa gacgcacata
    38401 tgcgggcgct tcaagcacag atcaatccac actttttata taatacatta gaatatatcc
    38461 gaatgtatgc aatcagcgaa ggaagtgaag aattagcaga tgttgtgtat gcgttttccg
    38521 ctttgcttcg aaacaatacg aatcaagaga aaacgatcac tttaaaagaa gaactggact
    38581 tttgcgaaaa atatgtctat ttgtatcaaa tgcgttatcc taatcgagta gcctatcatt
    38641 ttatgatcga tcctgatttg gaaaagatcg aagtacctaa atttgtgatc cagccattag
    38701 ttgaaaatta ttttaaacac ggtattgatt ttactcgatt cgataatgcg ctaagtgtca
    38761 aagtcttgca ggaaggcaaa cgggtaagga tcatcatcaa agataatgga aaagggatga
    38821 ctgaaaaacg gctaaaacag attgaagaaa aactgtctca tcctaaagtg gaacttcacg
    38881 gttctatcgg tctgcaaaac gtgaatgaac gtttacgtgc cagcttcggc agcagttatt
    38941 atatgtcgct tgagaataat gagacaggtg gactgaccgt ttctattaca ttcaaggagg
    39001 gatgagcgtg tatcaagtgt tattggtaga tgatgaatac atgattttga atgggttaaa
    39061 aaagataatt gattggcaaa gtttaggctt tcaaatagta gcaacagcag agaatgccaa
    39121 agaaggacta gctgtacttg aacagcgaca gattgattta gtagtaacgg atgtgacaat
    39181 gccagaaata aatggactgg aatttattga agcggctcaa aaagaacgtc ataattttga
    39241 atttatgatt ttatcaggat accaagaatt cgattatttg aaaggaggta tgcaattagg
    39301 cgccgtgaat tatttgatga aacccgtgaa taagttcgaa ttggttgaaa gcctcaagaa
    39361 aatcaaaaca cggttagatc agcaaaatga acaaaaaaat caacaagaaa tctatcaaga
    39421 actgttattc agtcaatggt tgaatgaaga attggatgaa ataagtgaag aagaaataat
    39481 aggacaaata agtgagaagc agcataggat agtattgatt caactggcaa gaactcatga
    39541 tactcttatc aatagatggt tgcaagaacg gcaacaaatc ttttactatc aacgaaatta
    39601 cggagaccgt gtattgatca cgttgttgtt ggaggcagat gaagttgatg gcttctgttg
    39661 ttttgttcaa gaaaactttc ctgatcagga atggctgatc agtatcgggg aagagacaga
    39721 ttctttggac aaaatccctg aaacattcca acaagcgaaa gacaatctac agttgcatca
    39781 attttatgga gataaagacg agcatgtttt ttacgcagta caagcagaaa caaaagatca
    39841 aaccatcgat ttttcaagtt tcagacgcgt gctgcaaaat aaacaattgg aagtcgctca
    39901 aaaaatgatc actgattttt ttgaacagtt tcagttagca gccatgatgc cggaagatat
    39961 acgatattcc agttttttac tgtttatgga gattcaacgg gaattgattg atctggaaga
    40021 tgaggaatat ttacaaggaa tcgaaaaaat ccatcaggca aaaacggtgc aagaacttca
    40081 gcagctactt ctgtcattca tcaaaaagca tcagagacag aaaaagtaca gtgacaatgt
    40141 ggaaaaagta atcgtaatcc tgcaccagca ttatcaagaa ccacttacac tcaaagaagt
    40201 gtcagaaaac ctgcatttaa atgtcatgta tctaggacag ctgttcaaaa aagaaacgaa
    40261 aaaaagtttt tcagcttatt tgaaccattt acgtatggaa aaagcgaaac aactgctttt
    40321 gcacagtaat caaaacatca atgagatagc aagtgagatt ggctacaaca acaccacgta
    40381 tttctctaaa ttattcaaga aaatcgtcgg cagatcccct aaagagtacc gagaaaacag
    40441 tggagaatca taaaagaaaa agccaatgta atgaaataac ttataaaaaa ttgtctagaa
    40501 atctaatttt gggctgttct tattacttga ggcaaggcag ctttttcatg acctctcgat
    40561 aaacggagtt caaaagccag tttctcggta ttaagccaaa tttatgcaaa aatgtggaaa
    40621 gccattttca caaaattttt cttaatactc gaaactaaac agctttttca cgacctctcc
    40681 actaaacggt gttcaatcag ctgaccctcg gtattagctc aaaaaatggc aaaatatgag
    40741 gaactatttt gccatttgtc tctctaatac tcaggtcaga acgctgattt cacaacctct
    40801 caataaacgg tgttcaaaag cttgcatctg cggtaataag accaaccaaa cgaaaaatat
    40861 gaggatctat tttt
//
LOCUS       DANKEF010000021        38023 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000021 DANKEF010000000
VERSION     DANKEF010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.021"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(451..1761)
                     /locus_tag="PWZ48_001257"
     CDS             complement(451..1761)
                     /locus_tag="PWZ48_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2260925.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="PWZ48_001258"
     CDS             complement(1773..3191)
                     /locus_tag="PWZ48_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2260926.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="PWZ48_001259"
     CDS             complement(3431..4999)
                     /locus_tag="PWZ48_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HDL2260927.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="PWZ48_001260"
     CDS             complement(5003..5413)
                     /locus_tag="PWZ48_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HDL2260928.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="PWZ48_001261"
     CDS             complement(5410..6333)
                     /locus_tag="PWZ48_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HDL2260929.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="PWZ48_001262"
     CDS             complement(6330..7208)
                     /locus_tag="PWZ48_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HDL2260930.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="PWZ48_001263"
     CDS             complement(7195..8532)
                     /locus_tag="PWZ48_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HDL2260931.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="PWZ48_001264"
     CDS             complement(8535..9638)
                     /locus_tag="PWZ48_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HDL2260932.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="PWZ48_001265"
     CDS             complement(9712..11220)
                     /locus_tag="PWZ48_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2260933.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="PWZ48_001266"
     CDS             complement(11186..11878)
                     /locus_tag="PWZ48_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HDL2260934.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="PWZ48_001267"
     CDS             complement(11900..12874)
                     /locus_tag="PWZ48_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2260935.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="PWZ48_001268"
     CDS             13174..14046
                     /locus_tag="PWZ48_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2260936.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="PWZ48_001269"
     CDS             complement(14504..16702)
                     /locus_tag="PWZ48_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2260937.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEIEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="PWZ48_001270"
     CDS             16934..17926
                     /locus_tag="PWZ48_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260938.1"
                     /translation="MVTIKDVAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="PWZ48_001271"
     CDS             17986..19407
                     /locus_tag="PWZ48_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDL2260939.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSLPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="PWZ48_001272"
     CDS             19400..20899
                     /locus_tag="PWZ48_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDL2260940.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="PWZ48_001273"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="PWZ48_001273"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2260941.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="PWZ48_001274"
     CDS             22491..23174
                     /locus_tag="PWZ48_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HDL2260942.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRPP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="PWZ48_001275"
     CDS             23176..24012
                     /locus_tag="PWZ48_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDL2260943.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="PWZ48_001276"
     CDS             24023..25777
                     /locus_tag="PWZ48_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2260944.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="PWZ48_001277"
     CDS             complement(26053..26394)
                     /locus_tag="PWZ48_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2260945.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="PWZ48_001278"
     CDS             complement(26462..28633)
                     /locus_tag="PWZ48_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HDL2260946.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="PWZ48_001279"
     CDS             28802..29701
                     /locus_tag="PWZ48_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2260947.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="PWZ48_001280"
     CDS             complement(29772..30629)
                     /locus_tag="PWZ48_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2260948.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="PWZ48_001281"
     CDS             complement(30614..33316)
                     /locus_tag="PWZ48_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HDL2260949.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="PWZ48_001282"
     CDS             complement(33329..34618)
                     /locus_tag="PWZ48_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HDL2260950.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="PWZ48_001283"
     CDS             34768..35814
                     /locus_tag="PWZ48_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2260951.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     SYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="PWZ48_001284"
     CDS             35816..37969
                     /locus_tag="PWZ48_001284"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HDL2260952.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
BASE COUNT        11224 a         7827 c         6751 g        12221 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgatct gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaatttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tgtcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcctccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtcc gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg gcgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgctt caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatagtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaa
//
LOCUS       DANKEF010000022        37385 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000022 DANKEF010000000
VERSION     DANKEF010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37385
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.022"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..615
                     /locus_tag="PWZ48_001285"
     CDS             <1..615
                     /locus_tag="PWZ48_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2260953.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            612..1385
                     /locus_tag="PWZ48_001286"
     CDS             612..1385
                     /locus_tag="PWZ48_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HDL2260954.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1382..1780
                     /locus_tag="PWZ48_001287"
     CDS             1382..1780
                     /locus_tag="PWZ48_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDL2260955.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1965..2636
                     /locus_tag="PWZ48_001288"
     CDS             1965..2636
                     /locus_tag="PWZ48_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260956.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2695..3135
                     /locus_tag="PWZ48_001289"
     CDS             2695..3135
                     /locus_tag="PWZ48_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260957.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3147..3815
                     /locus_tag="PWZ48_001290"
     CDS             3147..3815
                     /locus_tag="PWZ48_001290"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2260958.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3879..4502
                     /locus_tag="PWZ48_001291"
     CDS             3879..4502
                     /locus_tag="PWZ48_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HDL2260959.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4558..4944
                     /locus_tag="PWZ48_001292"
     CDS             4558..4944
                     /locus_tag="PWZ48_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HDL2260960.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5157..6548
                     /locus_tag="PWZ48_001293"
     CDS             5157..6548
                     /locus_tag="PWZ48_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HDL2260961.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7159..7527
                     /locus_tag="PWZ48_001294"
     CDS             7159..7527
                     /locus_tag="PWZ48_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDL2260962.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7707..8648
                     /locus_tag="PWZ48_001295"
     CDS             7707..8648
                     /locus_tag="PWZ48_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDL2260963.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8717..9448
                     /locus_tag="PWZ48_001296"
     CDS             8717..9448
                     /locus_tag="PWZ48_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDL2260964.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9592..11772
                     /locus_tag="PWZ48_001297"
     CDS             9592..11772
                     /locus_tag="PWZ48_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDL2260965.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11792..12274
                     /locus_tag="PWZ48_001298"
     CDS             11792..12274
                     /locus_tag="PWZ48_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HDL2260966.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12290..13051
                     /locus_tag="PWZ48_001299"
     CDS             12290..13051
                     /locus_tag="PWZ48_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2260967.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13182..14516
                     /locus_tag="PWZ48_001300"
     CDS             13182..14516
                     /locus_tag="PWZ48_001300"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDL2260968.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            14823..15308
                     /locus_tag="PWZ48_001301"
     CDS             14823..15308
                     /locus_tag="PWZ48_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HDL2260969.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            15301..15984
                     /locus_tag="PWZ48_001302"
     CDS             15301..15984
                     /locus_tag="PWZ48_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDL2260970.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            16110..18755
                     /gene="polA"
                     /locus_tag="PWZ48_001303"
     CDS             16110..18755
                     /gene="polA"
                     /locus_tag="PWZ48_001303"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDL2260971.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            18800..19636
                     /gene="mutM"
                     /locus_tag="PWZ48_001304"
     CDS             18800..19636
                     /gene="mutM"
                     /locus_tag="PWZ48_001304"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HDL2260972.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            19600..20229
                     /locus_tag="PWZ48_001305"
     CDS             19600..20229
                     /locus_tag="PWZ48_001305"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDL2260973.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            20281..20613
                     /locus_tag="PWZ48_001306"
     CDS             20281..20613
                     /locus_tag="PWZ48_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDL2260974.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            20925..21416
                     /gene="nrdR"
                     /locus_tag="PWZ48_001307"
     CDS             20925..21416
                     /gene="nrdR"
                     /locus_tag="PWZ48_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HDL2260975.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            21439..22812
                     /locus_tag="PWZ48_001308"
     CDS             21439..22812
                     /locus_tag="PWZ48_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HDL2260976.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            22812..23738
                     /gene="dnaI"
                     /locus_tag="PWZ48_001309"
     CDS             22812..23738
                     /gene="dnaI"
                     /locus_tag="PWZ48_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HDL2260977.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            23867..24307
                     /locus_tag="PWZ48_001310"
     CDS             23867..24307
                     /locus_tag="PWZ48_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2260978.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            24451..25152
                     /locus_tag="PWZ48_001311"
     CDS             24451..25152
                     /locus_tag="PWZ48_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HDL2260979.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            25149..26270
                     /locus_tag="PWZ48_001312"
     CDS             25149..26270
                     /locus_tag="PWZ48_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HDL2260980.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFEFWKQEENWDINFFVSETNTNPFQFN"
     gene            26285..27517
                     /gene="pepT"
                     /locus_tag="PWZ48_001313"
     CDS             26285..27517
                     /gene="pepT"
                     /locus_tag="PWZ48_001313"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDL2260981.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            27649..28557
                     /locus_tag="PWZ48_001314"
     CDS             27649..28557
                     /locus_tag="PWZ48_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HDL2260982.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            28592..28864
                     /locus_tag="PWZ48_001315"
     CDS             28592..28864
                     /locus_tag="PWZ48_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HDL2260983.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            28875..29921
                     /locus_tag="PWZ48_001316"
     CDS             28875..29921
                     /locus_tag="PWZ48_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HDL2260984.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            30172..32781
                     /gene="clpB"
                     /locus_tag="PWZ48_001317"
     CDS             30172..32781
                     /gene="clpB"
                     /locus_tag="PWZ48_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDL2260985.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(32932..33618)
                     /locus_tag="PWZ48_001318"
     CDS             complement(32932..33618)
                     /locus_tag="PWZ48_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2260986.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(33596..34090)
                     /locus_tag="PWZ48_001319"
     CDS             complement(33596..34090)
                     /locus_tag="PWZ48_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HDL2260987.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(34110..35330)
                     /locus_tag="PWZ48_001320"
     CDS             complement(34110..35330)
                     /locus_tag="PWZ48_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2260988.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            35477..37312
                     /gene="lepA"
                     /locus_tag="PWZ48_001321"
     CDS             35477..37312
                     /gene="lepA"
                     /locus_tag="PWZ48_001321"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDL2260989.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        12417 a         6549 c         8100 g        10319 t
ORIGIN      
        1 tttgtttttg caccgttaga aggagaagac ctcgcttcat ttctggttgg tgcggcaatc
       61 atgatgatcg gaatgacgct ctttttgttt ggagcagatt attctatgat ggaagtcgga
      121 gatctagtag ggaaatatat gatcaaaaag aaaagtttga ccgttttggt cagtttggga
      181 tttgctatcg ggatcgtgat cacgattgct gaaccttccg tgcaagtttt gggacaacaa
      241 gtatatgata tatctgacgg agagattggc agagtgttgc tgattgggat cgtcagcgtc
      301 ggcactggag tatttctagc ctttgcgctg cttcgggtcg tattcaagct atcctattat
      361 caattgatgg ctataggcta cataggcgtt ttgatcgcct catttttcac cagttccgaa
      421 tttatgccga ttgcttttga ttcgggggga gtgactactg gtccagtaac tgttccattt
      481 atcttggccc ttgccggagg gatgactagt atgatcaaac aaaagaaaaa tgaaaatgac
      541 agttttggaa tggtcggtat tgcttcgtta gggccgattc ttgccgtgat gattttgggg
      601 gtgatcttcc aatgagtttc tggcagcaag tgatagatgg aatcgaaatc gttaccatgg
      661 aagtcatttt ggcagtggca cctatcgtaa tcctatttgt tttaatgaat atcattagtt
      721 ttcgtatgcg caaaaagcgt ttctttgaca ttatgaaagg tttcttgata acgactattg
      781 ggttgatcct tttcctgcac ggagtcaata tggcttatgt accggtagga cagcatttag
      841 gcagtgctat tgcgggattg ccttacaatt atatactggt tccccttggc tttttaatgg
      901 ggttcttagt agggtttgct gagccggcga ttcatgtaat gatcaaacag gttgaagaat
      961 tgagcgaggg gagaatccga tcgaaaatca tgctgatggt aatttcaatt gggatcggcg
     1021 cagcagtagg attgtctatg tggcgtttgc tcgtcggatt ctctctttat tatttcttga
     1081 tcccaggata cgggctagca tttattttag gacgaaaagt ggacaagatc tttttagcaa
     1141 tggcttttga taacggtgga gtagcaacgg gcccgatgtg ttcgacattt attttatcaa
     1201 tgagcgtggc aatcgctgca caaattgaag gaagagatcc gatcattgac gggtttggtg
     1261 tggttgcaat gattgcattg acaccaatat tatccacttt agtactagga tatatttata
     1321 aacgaaaaga tgaaaaagac agaatcgaaa aagaaagatt gcaaaaattg ggggaaagca
     1381 aatgaaagat cttgtagcat tgaatcttca catgattgtg acgatcgttg acggaggaat
     1441 gggcgaagat gttatcgact tttcaaaaga agcaggtgca gcaggcggaa cgatcttaca
     1501 tggaagaggt tctggtgtac atgattcagg gaaattttta ggtttggaca tttcgccaga
     1561 aaaagatatt gtgttgacat tagtacccga tagtttgacg aataaagtaa tggatgcgat
     1621 tggtcaaggc atcaacatcg atattccagg taatggtatt tgttttagta ttgatgtaga
     1681 caaagtcatt ggtatcacta agattcatca atatcgagaa attatcggaa tgcaagaact
     1741 tttagatgaa aataacgata gagaaaagtc tgaatcatag acaagcgttt gaagaggaaa
     1801 taaacacaaa caagaaggcg gatgagcgtt atttccgctt ttttgtttgt atttttctcg
     1861 tgaatactta tgaaatagta aatttttatg aaattctact ctaagcatca gactttcttt
     1921 tcaaaatatg ctattttaaa aatgatgaaa aaagggggag aaaaatgaat tttttagttg
     1981 ttttaaaaga tactctgatt gagcgattta catttctata tgttcaattg caggaaaata
     2041 aaacattgct gcatttttca ccagagttcc atctagaggg gctaacatta ggagaaatct
     2101 atcaagcaga agggctgtcg gctgttttgc acttttttaa ggtagaattg gaattaccag
     2161 taaaagatta tatcgagctt tctcttgaat cttgggatcg agcagtggtt gaactgtttc
     2221 cagaaggttt aatgatcgat acaaacgatg ggaagatcca tctgactgca gcagaactgc
     2281 aaaaacaaat gcgttatcaa atagatgacg aaaattcttt ttctattttt agacgtcagc
     2341 aaaagattct aaaaagcttt ttaaaagtga tcagtaaaaa acggaatctg atcaaaacga
     2401 cacagctgtt gaaaaaatat ccaggtctgc ttcacacgtc tattcaattt cctcaattga
     2461 ttacgatgat ccatcgtttt atacgtatgc aacagctacc atctaagaag ctgtttcttc
     2521 cattgacgga cacatttcga gtagtcaaca gagaagataa aaagaaagtg attgtgattg
     2581 actttaccag aaatcggaat gtccttcacc aagttgcaat ggaaaaggcc aattaaggct
     2641 ttttcttttt ggattttttt gtcataataa atattagatg atggaggaga taatatgatg
     2701 caaacatggt tagaaaggct aatacgattt cagcagaact atcacaaact tatgcaggga
     2761 agatatgcga aatttgatca gttgaatcgc ggtctattga tcgctgcgct tgtctttagt
     2821 ttgctaagtc gttggttgcc ctatagaatc ggacaattgt tgttcatcgt ctttttcgga
     2881 tggatgatat ttcgtttcct atcaaaaaaa atctacccca gactaaatga gaatcaacgt
     2941 tatttgaaat atcttgatag agtcaaagaa aaatatcatc agataaaaaa caaaaagcaa
     3001 gtcaaaatgg atcgaaaaag ttatactttt tttgagtgcc cgaattgtca tcaaaagcag
     3061 cgtgcaccta aaggaaaagg acgaatacgt gtgacatgca aaacatgtgg tataaaattc
     3121 gaaacgaatg tttaagggag aaaagaatga aattagtatt attacgtcat ggcgaaagcg
     3181 aagcaaattt tgaaaattac tggacaggat ggctagatgt cgccttaaca gaaaaaggaa
     3241 aagaacaagc agcagttgcc ggtcaaaaaa tgcttgaagc cggacttcaa ttcgatgccg
     3301 tttatacctc tgttttgaaa cgtgcgattc ttaccggaca agtcgcacta gaagaaatgg
     3361 gtcagttatg gcttcctata ataaaaacat ggcgtctaaa tgaacggcat tatggggatc
     3421 ttgtgggtaa aaacaaagat aagatgaaag aaatatacgg aaaagatcaa gtaaaaacat
     3481 ggcgcagagg atatacggaa gttccgccgt taaccgcaga aaatagattt gaccgcagat
     3541 atgatcactt ggatccgcat ttgatcccat acggagaaag tctggaaatg acagtgaatc
     3601 ggatcattcc cttatggcaa gatcatctag caccaaaact aaaagatcgg caagatttac
     3661 tagttgtggg tcatggtaat agcatccgag cactgacaaa atatttagaa gatgttcctg
     3721 aagatcaaat ggatacgatc gatatcccaa atgcacaacc aatccagtat acattagcag
     3781 atgatttgac aattctggac aaaaaaatac tttaaagaac acctttatct aaaagtgaga
     3841 gatagaaaaa agaagcaaag gaatggaaca aaaggaacat gaaaaattat atatgggact
     3901 ttgatggtac attgtttgat acgtatcccg caatggtaga tggtgcatgg caagcactaa
     3961 aagattttgg tatcagtatg gacaaaaaag aaatctactt caaaatgaaa aagtattcta
     4021 ctagttattt gatcaatgaa tcaaacttga atgccagaga gtttaatgaa ttatttcacc
     4081 gatatgaaaa agaaagtacg gaagtttccc gtccatttcc agaaacgaaa caagtactgg
     4141 aaatgctaaa agacaacggc ggtcgtcatt tcatcctgac ccaccgtttg acagaatcaa
     4201 catggggcct gttaaaagaa catcgattgg cacatctgat tgaggaagta gtagggatcg
     4261 atcaggactt tcctcgtaaa cctgatccag catctttaaa ttatttgatc gatacttttc
     4321 atttagaaag aacagacacg atgatgatcg gcgatcgtcg tttagatatc gaagcaggga
     4381 aaaatgcagg agtcgctact tgtctttacg acatcgatca ttttctgggt gagatcccgg
     4441 cagattatgt ggtaggaaat ctgaatgaaa tccttacctt gcagagcatc attaaaaagt
     4501 agtatcccct ttcaaaggag atatgttata attttcataa aggagtgata aaattatatg
     4561 gaaatcagaa gacaaaaaga agttgtagaa gcatttcatt atgatatgag attgccgaac
     4621 caagaagtag aaacagattt gaaagtaggc ttctcgcctt taaaagaaac gatcgaaaac
     4681 tatccgaaag aacatagcat cgtaggagca cgcttagagt tccgtttagt gtttgataca
     4741 tatgttttat ctggaagtgt gagtcagatc aatcatatcc tcaatcggaa aattgaaggt
     4801 caaaatgatg tcacacaaga agaagtggac gagctagtag caccgttatt cgatatcgta
     4861 cgccgaatgt cttatgaggt gacagagatc gccttagatc agcctggggt aaaattgaat
     4921 tttcaatcag aaaaacaaga atagacagta aaaacatacc tgcgatagat taaagctatc
     4981 acaggtatgt ttttttatta ctaattgtaa gcaaaaagaa tgtcatttcg ataaaaatcg
     5041 tctaaaatat aaaagtcact gaaaaaaatt ttttcaggag gatggttgaa gaatcattct
     5101 cgggaacctt tacttccttc gatggacttg tcattaataa tatgaggagg agaaaaatga
     5161 caacactcat cacagtttta gtggttctag tttttgcggc attattatat gtgttctatc
     5221 gtatgcaaaa gcgccataca aaattttcaa cacgcgtctt tacagcatta ggagttggta
     5281 tcgtctttgg cggtctgctt caattgatct ttggtgtaga cgacaaagtc gttactcaat
     5341 caatggaatg gatcgggatt gtaggtcaag gatatgtatc attcttgcaa atgctagtta
     5401 tgcctttagt gtttatttcg atcattggtg cattcacaaa aatgaaagtt tctgaaaagc
     5461 tagggaaaat tagtgcgact gtattgactt ctttacttgg aacaacagct attgcagcat
     5521 tgatcggaat tggttcaaca atgctgttcg gactccaagg agctaaattc acacaaggag
     5581 ctgcagaaac agcacgaatc tctgaactgg caacacgtca ggaaacagtt gaaaacttat
     5641 cgattccaca gcagatttta tcctttatcc ctaaaaacgt ttttgctgat ttagcaggta
     5701 gtcgtgcaac aagtacgatt ggagtcgtta ttttcgcggc atttgtcgga atcgcttatc
     5761 taggcgtgaa aagaaaagcg ccaaaagaag cagcattctt ttcagattta gtcgacagtc
     5821 tgtataaaat cattatgcgg atcgtgacgc ttgttttgcg tttgacacct tatggagtat
     5881 ttgctttgat gacaaacgtt ttggcaacaa gtgattttgc agctatcgtc aatttaggta
     5941 aatttgtcgt tgcttcttac gtagcattgc ttgctgtttt gattgtccac tcactgattt
     6001 tgattggtgc aaaagtcaat ccaatcaact atttcaaaaa agcctttccg gtattgagct
     6061 ttgcgtttac ttcacgttca agtgctggag ctttgccatt gaatatcgag actcagacaa
     6121 aagctttagg agtagatgat gcaacagcta atttctcagc aagttttggt ttgtctattg
     6181 gacaaaacgg ctgtgctggc gtttatccag ctatgttggc aacgatcgtc gcaccagcag
     6241 ctggagtgaa tgtctttagt ttggaattta ttttgatgct tgtggcaatt gtaacaatca
     6301 gttcttttgg tgtagctgga gtcggtgggg gcgcaacatt tgcttcactg atcgttttag
     6361 gggcaatgaa cttgccagta gcaatcgttg ggttagttat ctctgttgaa ccattaatcg
     6421 atatggcacg tacagcagta aacgtcagcg gtagcatggt agctggtatc gtgacaagtg
     6481 cacgcatcaa agatttagat cgcgatgtca tcaatgatga ttctaaagtg atcgatgtga
     6541 atgcatagtc aaaaagaggt tgtgatagaa gtggtcagct ttaagaaata agaagaagtt
     6601 tcctaaaaat agtttctcaa atttttgtga aatttcggct tatttccgag aagctacttc
     6661 tgaaacaccg tgaataagag gttgtgaaaa agctgccctg catcaagtaa taagaacagc
     6721 caaacaaaaa tagttcctca tattttacgt ttggttgagc ttattaccgc agatgcaagc
     6781 ttttgaacac cgtttaagaa agaggtcgtg agcctgccgt ttcactctga gtcacaaaga
     6841 gcaatttcaa aaaatagctt ctcatgtttg ttgaatattg tgtcttgtga ccgaggagtt
     6901 gtcaggagaa caccgtttac cagaggttgt gacttgtttt tagtcacgac ctcttttttt
     6961 atttattcca tataaataca cattatttat tccgaaaagt gaaagcgcaa ataacaaaaa
     7021 caaccaactt atttattatt aaagaaaagc gtatttaagt aggacgattc tgttgagagc
     7081 tttcgtagat tttcccattt tcctatggta aactgacaaa gttgagaatg ttagaacaga
     7141 tcgtttagga ggaaacagat gtttacgaat gaaataaaaa tcatgcttta tgtggacgat
     7201 gtagaaaaaa atgcaacgtt ttggagagca gttggctttg cggaaaaaga tcgtcaagaa
     7261 atggatggga cgcttataat tgaagtagga gttgaggaca gtcaaacctc atttctgtta
     7321 tatgatcgag catttgttga gtcccattca cctggcacag ctactagtgc cccatctttg
     7381 atgttttaca gtaaagatat ctttggacta tataaaaaaa tgcagacgca atcagtcaca
     7441 ttaggtgata tggtacagct tggagaagaa tatgtcttta actttgctga tcccgatggc
     7501 aactactttg cggttacagg taaataatag aaataaagac tttgtttttc tgtgcgaaac
     7561 aggaaagcga agtcttttta attcaaataa taatattatg taaacaaaaa actttccatg
     7621 cgtaattata cacaagcaaa taaatcgtca aatgcgaacg ccttctttat actgtttagg
     7681 aacgaaaaga aggaaggaag aaatacatgg attatcaagt actgctttat tacaattata
     7741 caaaaatcga agaaccagag gaatttgcca aaaatcattt agccttttgc aaatctttgg
     7801 atcttaaagg tcgtattcta gtagctgaag aaggaataaa tggtactgtg tctggaaccg
     7861 ttgaaaacac gactgcctat atgaaagcaa tgaaagctga tcctcgattt gctgatacga
     7921 tgttcaagat cgatccaagc gaaggacatg cctttaagaa gatgtttgta agataccgtc
     7981 cagagcttgt aagtttaaag ctagaagagg acattgatcc aaaccaagtg acaggtgcct
     8041 atctttcacc gcaagaattt aaagaagcta ttttagatga acagacagtg gtgattgatg
     8101 cacggaatga ttatgaatat gacttgggac attttcgagg ggctgtacgc ccagatatac
     8161 gcagttttcg agagcttcca gactggatcc gcgagcacaa agaagaattt atggaaaaac
     8221 gggtagtgac ctactgtact ggtggcattc gttgcgagaa gttttctggt tggcttgtcc
     8281 gtgaaggatt caaagatgtc ggacagctgc atggcggtat tgctacttat ggtaaggatc
     8341 ctgaagtcca aggagaatta tgggacggca agatgtatgt gtttgatgaa cggatcgctg
     8401 tcgatatcaa ccaagtagat aaacaaatcg tcggtcgcga ctggtttgac ggaacaccat
     8461 gtgaacgata tatcaattgt agcaatcctg agtgcaatcg tcagattctt gcttccattg
     8521 aaaatgaaga aaaacattta ggagcatgta gcatagagtg tgcgaaacat ccgcgcaatc
     8581 gctatgttca agaccatgga atctctccag aagaagcaga actgcggttt acagctttaa
     8641 ttaaatagaa aacaatagtt ttgattgtcg accatttcca atatgcgcta tactagcgtt
     8701 gaggatggtg atccacatga aatcattgat ttatgctatt ggggatgtcc atggcaaaca
     8761 tgagcttttt gaaaaactta ttcactatta tgatccaaag atccatcagc ttgttttatt
     8821 aggggatctc aatgatcgag gtcctcgtac aaaagaatgt tttttattag gaaagcaatt
     8881 agtcgaagaa acaggagcca tttacctccg gggaaaccat gaagaatatt ttttgcaatt
     8941 tctgcaatca ccggaggact ggtatgcgcc ttatatgaga aatggtggaa aagaaacgtt
     9001 tgaaagcctg attcacccag gggcatcgga ggaatactcc ccaactgaaa tctcgatgat
     9061 gatccaatcg agatattcag aactgattaa atttttagtc cagcgcccct tgtattatga
     9121 atgggaaaat tatctttttg tccatgccgg tgtggatttg acaaagaaag actggcgtaa
     9181 gacaagtcta agagacttta tttggataag ggaaccattt catgaaggga aaaacaatac
     9241 aggtaagaca attgtatttg gacatacgat tacaccaatg ctccatggag atatgcagac
     9301 aacagacctc tggattcagg atcataaaat cggaatggac ggtggggctg tattcggagg
     9361 gtcattgcat ggggttgttt ttgacaagga aggtattctt caagatattg aatatcagaa
     9421 caacgaagga ccttggcatg gaatctgact aatggaaaga atgaaggagc tgtgactttg
     9481 ttgcggctct ttttattttt atagagaata ttgtttcttt cagtagttta acccaggata
     9541 aaaagtatga tagaataaag aagcaatttt tagcagaaaa gaggcatttt aatgacggaa
     9601 aatttgaacc agacgatcat tcagcttgta caaaaagaac tttcagacta tcgtcctaag
     9661 cagttgacta ctgtacttaa tttgttaaat gaaggaaaca ctgttccttt tatcgcgcgt
     9721 taccgaaaag aaatgactgg aagtcttgat gaagtccaga ttcgtgaaat cgaagagcgg
     9781 tatgcctatc ttgaaaactt agaaaaacgt aaaactgaag tgattcggct aatcgatgaa
     9841 caagggaaac tgactcccga gttagaagca gaaatcaccc aagccgtgaa aatgcaacaa
     9901 gtggaagatt tgtatcgtcc atacaaacaa aaacgacgga caaaagcaac gattgctaaa
     9961 gaaaaaggac ttgaaccact cgcgaagtgg ctgatgcagc ttacagatgg agaggtgcaa
    10021 tctgaagcag aaaaatatat tgacaaagaa aaagaagtct cttctgccga agaagcgttg
    10081 cacggtgctc atgagattat tgctgaacaa gtcagcgata atgcaaaatt tcggacttgg
    10141 attcggtctt atacgtataa caaaggtatg tatgtcagca atgtcaagga tgagcaagca
    10201 gatgaaaaag gcgtatatga aatgtattat gactttgcag aacccgtcca taaaatggtt
    10261 tctcatcgaa tattagctac taatcgtgga gaaaaagaag atattctgaa agtattcttt
    10321 caagtagatg aagcagcaat tttagcttac ttggatcgcc aaatagtcaa aaatcctgcc
    10381 tctccttctt cttcattcgt cagagaagcc tatcaggata gttataaacg ctttattcag
    10441 ccagcaatcg agcgagaact gcgcaatgaa ttgactgaaa aggcagatga gcaagcaatc
    10501 gctattttcg gcgaaaacct ccgtaatctt ttgttgcaac cgccactaaa aggtaaagtt
    10561 gtcctgggat ttgatccggc ttatcgtact ggatgtaaac tagcagtcgt agatgcgaca
    10621 gggaaagttt tagcaatcga agttatttat cctcataaac ctgcagcgca agcaaaaaga
    10681 gaagctgctg gaccagcttt tatccaatta attaataaat atcaagtaga tatggtagct
    10741 atcggaaatg gtacagccag tcgggaatct gaactttttg tggctgagca gttgaaatcc
    10801 gccgaccata aaacctatta cgctatcgtc aacgaagcag gcgcttctgt ttactccgct
    10861 agcgaaattg cacgaaaaga atttccgcat ctacaagttg aagaaagaag tgctgtttct
    10921 attgcccgtc gcctgcaaga tcctttagct gaattagtca aaatcgatcc taaagctgtc
    10981 ggagtaggcc agtaccaaca tgacgtttct caaaaacgtt tggctgaaca attggatttt
    11041 gttgtggaaa cagcggttaa ccaagtaggt gtggatgtga atacagcaag cccgcagctc
    11101 ttgcagcata tatcaggatt gaataaaaca actgcccaaa acattgtcat ttatcgtgaa
    11161 gaaaacggag aattcaccgc acgaactcaa ttgaaaaaag tgccacgctt aggaccaaaa
    11221 gcatatgaac aagcaatcgg attcttgcga gtccctggtg ggaaaaacat tttggacaac
    11281 acaggtatcc atcccgaaag ttattctatt gcaaaagaaa tattaacttc agtccaactt
    11341 tcagaaaaag agcttggtac agaagaagca gtagaaaaat tgacacgatt atccgtagag
    11401 aaactggctg aaagcctatc agtcggagaa gaaacgttag ccgatatttt agctgggctg
    11461 acacaacctg gacgagatat gcgtgatgag atgccagcgc ccttattacg tacagatgta
    11521 ttgagtatgg aagacttgaa accagggatg gaattgactg gaacggtccg taatgtcatt
    11581 gacttcggcg catttgttga tatcggtgtg aagcaagacg gacttgtcca tatatcgaaa
    11641 ctcagcaaaa aattcgtcaa gcatcctaca gatgtcgtgt cagtaggaga tatcgtaaca
    11701 gtttggatag aacaagtcga tacgaaaaaa ggcagaatca gtttgacgat gctttcccct
    11761 tatgaagagt aacaatgagg agtaacaaaa attgacacaa gaagaactgc agcagttagt
    11821 agaagagata tcattaaaaa gcttccagct tcctttcaag caccaagcaa tgttcaatcc
    11881 acgcttacgt acaactggcg ggcgctatca tttaaacgat catcatttag attttaatgc
    11941 gaagctattt gaagagtcag acgaagagac gattgcagga atcatcaaac atgaactttg
    12001 ccattatcat cttcatctag ctggaagagg gtatcagcat aaagatatgg atttcaagta
    12061 tcttttgaaa aaaacaggag ggttgcgcta tgcaccagcc ttgacgaaga aacagataaa
    12121 gatggagtat tatcgatgcc aagattgttc tgccacgatc tgccgcaaga gaagaatcga
    12181 tgtacgcaaa tatcgttgtg gaagatgtca tggagaaatc aaatggattg agacaaaaag
    12241 agaagacttt aatgaagcac aaaaaatcag atgaaaggat agaaggcctt tggaaaaaag
    12301 tgagcagagg aatctaaaag actggaatca gctattaaat tttttagccg cacctgaaga
    12361 gggaaatttt gaggaactga cggcacctac acttatttta gctggaaact gtcttcctat
    12421 cttagctgat aaagcagcac aaatgtattt aaatggccaa gtagatcaat tgtttttggt
    12481 cggaggggtg gggcatgcga ctagaatcct ttatgagaat tttgaaaagc aaggatttca
    12541 ttttgaagat ggcatgagtg aaagtgaaat ctgtcgtcag tatctaaaag aagtctatga
    12601 tttgcccgat aaggcttttc tgatagaatc gaagtctaca aattctggag agaacgccac
    12661 tttttcactg gagattcttc attccttagg agctgtacca gaaaaaatct tattgatgaa
    12721 tgatccgaca ctgcaacgca ggactcgagc aacttttgag aaagtatggc aaaatgaaca
    12781 aactattttc gcgaatgctg tgccatttgt gccggaaatc cttcactttt ctgaagagat
    12841 catctttaca gcaaaagaac tgaatcatca atggcctaag gaatatttcc atgcgctagt
    12901 attaggagaa atggaacgtc tccatgacga tgagaacggc tatggtccga aaggaaaaga
    12961 ttttattcct cacattgata tatcagagga agtttggtct tcttacacca gaattaaaga
    13021 gtcaataaaa acggattttt ctcgtacttg accattttag caagcgaatc ctcctttcca
    13081 aataaatttg gttgaaattt tcaatcgttt ataagataat gaggagcgtg ttgaatgtaa
    13141 tagctaaaag aatggacgct agataagaga agaggattca aatggaaaat caaaataaat
    13201 tgtatcattt tgttggtatc aaaggttcag gaatgagctc cttggctctt gttttacatg
    13261 aacaaggtct aaatgtccaa ggatcagaca ttgaaaaata tttctttaca caaagagatt
    13321 tagaaaaagc aaatatcacg attctgccat ttaacgcaga taatgtaaaa ccagggatga
    13381 cgatcattgc aggaaatgca tttcctgatt cacatgaaga aatccagcga gcaaaagaat
    13441 taggtttgga agtgattcgc tatcacgact ttattggtca ttttatccaa aattatacga
    13501 gtatcgctgt aacaggctct cacggaaaaa caagtacgac tggactgctc tctcatgtat
    13561 tgagtggtgt ccgtccaaca agttatctaa ttggagatgg aacaggacat ggtgatccgc
    13621 aagcggaatt cttttcattt gaggcctgtg aatatcgccg tcatttcctt gcctattcac
    13681 cagactatgc aatcatgacc aatatcgatt ttgatcatcc ggattattat acaagtatcg
    13741 acgatgtatt tacagctttc caaacaatgg ctggacaagt gaaaaaagct atttttgcgt
    13801 atggtgacga tgcttattta cgcaaattaa aagcaaatgt gccgatttat tattatggtg
    13861 tcacagaaaa cgatgatatc caagcacgaa acatcgaacg cacaacttct ggttctgctt
    13921 ttgatgtcta tcatggggat gaatttgttg ggcacttcac tgtaccagca ttcggtaaac
    13981 acaatatctt aaatgcttta ggtgttattg cagtagctta tttcgaaaaa ctggatctga
    14041 aagaagttgc agaagaaatg ctgactttcc caggagtaaa acgtcgattc agtgaaaaga
    14101 tcgtagcaga catgaccgta gtcgatgatt atgcgcatca ccctgcagag atcaaagcaa
    14161 cgattgatgg cgcacgccaa aaatatccag ataaagaaat aattgcagtt ttccaacccc
    14221 atacgtttac gcgtacgatt gcattgatgg atgaatttgc agaagcactt gatttagctg
    14281 ataaagttta cttatgcgat atttttggtt cggctcggga agaacaagga aacgttaaga
    14341 ttgaagacct tggagcgaaa atcaaaaaag gtggagaagt catcaaagaa aataatgtat
    14401 ctccattgtt agattatcat gatgcagtag taatcttcat gggtgctgga gatgttcaaa
    14461 aatttgaaca agcgtatgaa aaacttttaa gcagcacgac gaaaaatgta ctgtaataca
    14521 attactgcag tgtccttata aaaatagaaa gagggggagt ggcaaaaatc ttgccaagcc
    14581 cccttttttg caacttatga taaacggtgt tcaaaagctt gcatctgcgg taataagccc
    14641 aatcaaatca aaaacatgag aagctgtttt ttcttggctg ttcttattat ttgatgcagg
    14701 acagcttttt cacgacctct gtaaactata ggaatctgtt aaatcaaggg agcagaactt
    14761 gccttttacg ctacttttgg tacaataatg tatgattaaa aataaactgg agggtttcta
    14821 tcttgaacat aggtaagccg agaaaactag gaaaaacaat aaatgaaatc gaggaaggcg
    14881 actcgttatc cttaacagaa tctattgaag ataaccagct tttgctgtat ttgggattga
    14941 cgaatgatgc aaatcctttg tatatccaac atgactttgc acaaaaaaca gaatatcaac
    15001 agccaatcgt tccatcagtg atgctgatgg ggatcatcac aagtgcaatt tcaaagcatc
    15061 ttcctggacc aggatcacat gttgtcaatt tttctgtcaa ttttattgaa ccggttttcc
    15121 attatgaaac actgactttt cagtttgagg tcataaaagt agataagatg aaggatgtgg
    15181 tgacgatttc tgtagaagcg gagaatacga acgaagatcg tgtgttggat gctgtcgtta
    15241 tggtccaacc gccacaagtt cctgaattgg aaatagcaat cgaaagtgaa ggtgaattga
    15301 atgaataatc caagtacatc aatgtcaggt atcaatcgtt tttatgcaaa agtttatgga
    15361 tttttaggta tgggacttgc ccttagtgca gtcacttctt acttggctct ttgggtattt
    15421 cctttgcaat tagcaacttt tatcaataat tttccactag gctttgtggg tctgtggatc
    15481 gttgaactga ttttagtaat ggttctaggc gtaaaagcac aaaagaatcc gagcttagca
    15541 atcggtggat ttattgccta ctctcttttg aatggcataa cattagcggt tactcttgca
    15601 tactacgata ttggaacagt aacgcaagct ttcttgacag ctactttgat gttcatgggg
    15661 atgtctgttg tcggagtaac aacaaaaaga gatcttagcg gggttggaag agcaggacag
    15721 gcagctttaa tcggagtgat cattgcacta gtcttgaaca tcttcatttt gagaagtagc
    15781 gtagttgagc tgttcatctc cctactgtta gtcgtcatct ttgcagggat tacagcttat
    15841 gataaccaaa gcatccgtaa tatctatgtc cagacaaacg gaacagcaca tagtggaatt
    15901 gctgtattca tggcattgaa gctgtatttg gactttatca acttgttctt ggcgtttctg
    15961 agaatctttg gacggaacaa ctaaaaaaga aaaaaattca tcagttgggg ctataccgaa
    16021 ttatttggta tggtcccgct ttttcttttc gcattccgca ggattctttg ttagaatgaa
    16081 taagaacttt acagtaagga gttaagaaga tgacaaaaaa taaattatta ctcgttgatg
    16141 ggaacagtgt ggcttttcgc gcattctttg cgcttcacaa ttctcttgaa cgtttcaaaa
    16201 atagaaacgg tcttcataca aatgcaatct atgcattcaa tacgatgttt gaaaatgtta
    16261 tgcaaaaaga gcaaccgact catgttctgg ttgcgttcga tgcaggaaaa acgacttttc
    16321 ggacagaaat gtatgcggag tacaaaggtg gaagatccaa aacacctggc gaattcaaag
    16381 aacaaatgcc atatatacgt gatttgttga caggactagg ggtacagtat tacgaattgc
    16441 ccaattatga agcagatgat attatcggta ctttggcaga aaaagtagac aaagatcaat
    16501 ttgatgtcgt cgtgctttca ggtgatcggg atttgactca attagctagt aaagaagtaa
    16561 aagtggatat cactgtaaaa ggtgtcagcg atatcgaaag ctatacacca gaacatgtag
    16621 cggaaaaata tgatgggttg acgccaaaac agattattga catgaaggga ctagccggag
    16681 atgcctctga caatatcccc ggtgtgacta aaatcggtga aaaaacagct atcaagctat
    16741 tgaaacaata tggctcagtc gaaggaattt atgaacatat cgatgaaatg aagcaaagca
    16801 aaatgaaaga aaatctgata aatgacaaag agcaggcttt cttatcgaaa aaattggcga
    16861 ccatcaatac ttcagcgcct gtggatgtct ctatcgactc attgaagtat gaagggaaaa
    16921 atctcgataa actcgttccg ttttataaag aaatggattt caatcagttc ctttctaaat
    16981 tgaatatcgt agaagaagaa gtaaaaatgg atgatatttt gtttgaagtc gttcatgaat
    17041 tcaaagagga aatgtttact acggatatgg cgttgtacgt ggaaatgatg ggcgacaatt
    17101 atcatacaga ggaaatcgtc ggagtagcgt gggggacaga aaagaaaatt tatgtcacga
    17161 acgatctatc tgtattcgaa aaccgagcgt tccattcttg gatcactgat ccgactcggc
    17221 tgaaaaaagt atatgatgcg aaacgaactt atgttgcttt aaaccgctat actggaaaaa
    17281 cgaaaggaat cgattttgat gtcttgctag gtgcatacct tctcgatacg aatgaaaaaa
    17341 gcacagatat cgaaggaata gctgctcatt acggatacaa cgatatccaa tcagatgaag
    17401 ctgtatacgg caaaggggcg aaaaaaggtt taccagaaga agaggaactc ttttttgctc
    17461 atttagcgcg aaaagtagct gcaatcaatg cgttgacacc aaaattgagc caagaactgg
    17521 cagaaaaaaa ccaagaagat cttttccgga aaatggaact gccattgtct atcattttgg
    17581 cagagatgga aataagcggg atcaaagtag atgcgactcg cttacaagaa atgaagggcg
    17641 agttctctgc tcgcttaaga gagatcgaac aaaaaatcta cgaagaagcc ggcgaagaat
    17701 tcaatttgaa ttcaccaaag caattaggag tgatcttatt tgaaaaaatg ggattacccg
    17761 ttatcaaaaa aacaaaaact ggatattcca cagccgtaga tgtactggaa cagcttcgtg
    17821 aacaagcacc aatcgtagag gatatcctca cctatcgtca aattgcaaaa attcaatcta
    17881 cctatgtcga gggactgctg aaagtgatcc aatcagatgg aaaagtgcat acccgttatg
    17941 tccagacatt gacccaaaca ggacgattaa gctctgttga tcctaatttg cagaacatcc
    18001 cgattcgatt agatgaaggg agaaagatac gtcaggcatt tgttcctaga gaaaaagact
    18061 ggctgattta ttcttctgac tattcacaaa tcgagttacg cgtacttgcg catatctcaa
    18121 atgatgaaca tttgaaagct gccttcctag aaggtcaaga tatccatgcc agcacagcga
    18181 tgcgtgtatt tggaatcgaa aaggcagagg atgtcacacc aaatatgcgt aggcaggcaa
    18241 aagcagtgaa ttttggtatc gtgtatggca tctctgatta cggactttca caaaatctag
    18301 gaatcagccg aaaagcagca cagcaataca tcgatacgta ctttgaaaaa tacccaggtg
    18361 tcaaagaata tatggaaaga atcgtacgcg aagcaaaaga ccaaggctac gtcgaaacgc
    18421 tgtatcaccg ccgtcgatat ctgccagaca tcaattcaag aaattacaat atccgttctt
    18481 ttgcggaacg aacagctatc aatacaccaa tccaaggaag tgctgctgat attttgaaaa
    18541 tcgcgatgat cgaattggat aaacgattaa aagaaacagg tcttcaagct acgatgctgt
    18601 tacaagttca tgatgaactt gtcttcgagg taccggaaaa agaacttgaa tcattagata
    18661 aattggtgaa ggaagttatg gaacaagctg tttctcttca tgtaccactg atcactgaca
    18721 gcagttgggg aaaaacgtgg tacgaagcaa aataaacatt gtttaagaca gagcaatata
    18781 atgaaggtga aaagaaaaaa tgccagaatt accagaagta gaaaccgttc gtaaaggatt
    18841 ggaacggtta gtagtaggaa aaacgattca aaaagttcag gtcttgtggc ccaagatcat
    18901 cgagcaacca gagacaccca tctttgaggc ttctcttgtt ggcgaaacca tccaatccat
    18961 tggacgtaga gggaagtttt taatttttca tctcgatcat tgtgaactga tctcacactt
    19021 gcgaatggaa ggaaaatatc aatttacgaa agaaaataca ccaatcgata aacatacgca
    19081 tgtacttttc ttttttgagg atggaagcca acttcgctac aacgatgtac gaaaatttgg
    19141 tcgtatgaca atcgtcgaaa aaggagcaag tgcaacttac cgaggaatca tgaaattggg
    19201 acctgagcct ttgccagata gcttcttatt agcagatttc gcaaatggac tgaaaaagtc
    19261 ccacaaagca atcaagccat tgctgctaga ccaacgtcta gtgactggtc taggtaatat
    19321 ttatgtagat gaagcattat gggaagcgaa gatccatcca gaacaaccgg cgaacacact
    19381 gcgccccaaa gaggtagaac aattacgtct atcgatcatt gatgttttgg atcgtgcaat
    19441 agaagccgga ggtaccacga ttcgcagtta tttgaatgca ttaggagaat caggcggatt
    19501 tcaagtagca ctgcatgtct atcagcaaac tggcaaacca tgtatacgtt gtggaacgcc
    19561 gatcgtgaaa acaaaagtgg cacaaagggg gacgcactat tgtccgaatt gtcaaaggct
    19621 aaaaatcgca aagtaggatt tgttttaggt ttgacgggtg gaatagctac cggtaaaagt
    19681 acggcagcca aagtctttca gtctcatgga tttcctttga tcgatggcga tgtgattgcc
    19741 agagaaactg ttgagcctgg aacgcttgct ctgaggaaaa ttgtatcagt gtttggccaa
    19801 gaggtattgc agcctgatgg gcaactagat cgagggaaac ttgggatgat tgtttttcca
    19861 tcaaaggaaa aacggcaaaa acttgatcag ctattagatc cgtttatccg aaaagcgata
    19921 aaagatcaaa tcgcctctct aagttccaat catcctttag taattgtaga tatcccattg
    19981 ctctacgaag gtcattatga ccactatatg gatgcagtag ctgtcgttta tactacgcca
    20041 gaaactcagc ttcagcgttt gatgaaacgt aatcaactga cgctagaaca agcgcagcag
    20101 agaattagta gtcaacttcc aatcgaagaa aaaaagcagc gtgcggatat tttgttcgac
    20161 aacaatggaa caaaagaaaa acttgtggga caaatcgaaa attggctttt tgctaatcgc
    20221 tttctttgag tatctgtgtc aattttgcta tcctgagtat atagaaagtg agggatagcg
    20281 atgggatcat taaaaaatca aaaggataaa gtcgtaggta acgcaaaaga aaaaatcggc
    20341 gagatgctca ataaagaaaa tctaatcgat gaaggtcgta cgcaatcaga aacagcagaa
    20401 gaaaaagaaa agcagtacca agcaagaatc gatgaaatag aagaccagca ggcagaagaa
    20461 gaagcgaagc ggcaagattt gactcaagaa cgaaaaaatc cgacacctga tcgtagtaaa
    20521 acaccgctcc cagaaagtga ccacattccc gatgagcaaa aaatcgagga acagcgaatg
    20581 aaacactata caggaatcga agagaagtta taaaatagta aaaaatcgag atgtgacttg
    20641 aagcagatga gaaggtgttc atctgcttca agttttttgt gctagatgac taaatcagct
    20701 tttgtgtaag ttatttctca gtaatcaagg tgatcagtat tggaaaattg aaatttagta
    20761 aggacagggg acacaattac gcatgaaagt agaaaaaatt gttcttatat ccgtgttttt
    20821 tgggtaggac agtcgcgttt gtgatttgtt tcgtgttata atacaactag ttgtaaagaa
    20881 agacacatcc tttatataaa atgcgatgca agaggtgaga caaaatgaga tgtcccagat
    20941 gtcaacataa taattcacga gtaatcgaca gccgtcaagc agatgatgga cgtgcgatcc
    21001 gtcgccgtcg cgaatgtgag aattgttcgt tccgattcac tacatttgaa cgaattgaag
    21061 ctgctccgtt attagtaatc aagaaaaacg gggaacgcga agagttcaat cgcgataaaa
    21121 ttttaagagg actcatccgt tcagcagaaa aaagacctgt agctatggaa caaatggaac
    21181 aaatcgtcaa ccatgtcgaa aaccgcgtac gcagtctcgg agaaaacgaa gtatcgacga
    21241 atctaattgg tgaatatgtg atggaagatt tagttgattt agacgaaatc gcttatattc
    21301 gttttgcaag tgtttatcgc cagttcaaag atatgtctgt tttcttaaaa gagttgcaag
    21361 atatcgtgga taaagcaaaa gaaaataata aagagaataa taaagaaaac aattaagaaa
    21421 caaaaaggag gcatttcctt ggaaaatgct tggaaagagc ttcaaccaaa aaatatttac
    21481 caaggaagaa aagcggctcc gctgagagaa gacagcaaga acgccttgct gtatctttat
    21541 cagccactaa tcggcggaga agccttgagt atctatttca ccctgttatc agaaatctct
    21601 caagaatctg gcaaaggtcc tgaagggatg catgcagatc tgcttagtag tctaggctgc
    21661 ggtatcccgc aattttatga tgcaagaaaa aagctagaag gaatcggctt actggatgtt
    21721 tattttaaag aagatagtga tctgggacct cgttttattt atgagctgtt agaaccactc
    21781 attcctaaag catttttcaa agactctgtt ctggcttttc ttcttctgga aaaagttggt
    21841 gaacgacgat tccagaagct tacccaattc tttgaaccta aaaggcatac agcagaggga
    21901 tatcaaaaaa tcacaaaaaa atttttagat gtttatcgct tcaatgaagc agtgtacgct
    21961 gctaatcaag aaaacatcga atcgctgaat aaatcttttg aagaatctga atcagtagca
    22021 ttacttgaag cagattcgac attcgattgg cagtttttga atgattggct ggcaaaaaaa
    22081 catattgcaa gtcttgatga tccaatcatc aaacaagtcg acatgtatca tcagctttac
    22141 ggtttcgatg aattgacact tggcgaactg atcagtcaat cttatgactt tagtgaacaa
    22201 aaagtatctg aaaaagaact gcaaaaagcc attttggcaa tgcagagacg tccggtgaag
    22261 caaacaaaag aaacagttgc ggtttctgaa aacagtaatg aaccctcgtc aaacgaacat
    22321 ttgacaaacg cagcgcttca gctgataaaa gaagcacaga gcattccacc gatgaaatat
    22381 ctggaagcaa tcaagtcaga aaaaggcgga tatgtttcta aacaagaaaa ctggttactg
    22441 caagatcttg tttctcaatc aggtctttcc agtagcgtga tcaatgtttt gctgaattat
    22501 gtactagtga tcaagaataa tgctagctta aatgccagtt atgtaaatac agtggcaaat
    22561 gaatgggcac aaaagaaaat cgccacagca gaagaagcga tccagcacat tcggcaaata
    22621 agtaatcaag caaaacaatc aaaacaaaag aagcaaacaa attatcaaac tggcaagcga
    22681 aatgttcgac gggaaaagct tcctgattgg gtcaaccagc caaaagatga aaaacagatc
    22741 agcagcgaga aaaaagcaga gattgaccgc cgttttaaag aatacctggc aaaaaaggag
    22801 ggtgatatct agtggaagac gtaggcaaag aattgaagag gatcatcagc aaacgcaatt
    22861 atcaagaaaa gtatgctgaa atgatcgctg aagtcttaaa tgatgcggat gttcaagctt
    22921 ttctaagcgc acaccaagaa gaattgacac aagcggatat tgaaaaaagt tacgctaaat
    22981 tgtatgaatt cgtgcaagaa aagcgtaaat atcaaaaaaa cgatccaacg atgatcgctc
    23041 ccggctatga gccgaagctg acgttgaatt ttcattatgt ggatgtcact tatgtaccaa
    23101 cggaagcatt gttagcacgg caaagacaag aagaaatcag aagccgagta aaagcaatgg
    23161 atatgccaaa agatattcaa tcggcttctt ttgaaaactt tgaacgtaca gatggacgag
    23221 gatatgcgag cttagaagcc ctagattttg tagagaaata tactgctgat ccaaaagggt
    23281 atcataaagg actgtatcta gtaggaagtt ttggtattgg aaaaacatat ttattaggag
    23341 caacagcgag agaattagcg attgctggat tcaccacgac gctagtccat tttccgacat
    23401 ttgccgttga gatgaaacag gcaatcggaa aagatcaagt ggcagaaaag ttagatgcag
    23461 tgaaacgttc acctattttg atgatcgatg acattggtgc agatgcgatg agtagttgga
    23521 tacgagacga agtattcggt gttatccttc aatatcggat gcaggaacag ttaccaacct
    23581 tcttctcatc gaattttaca atggatgaat tggaacagca tttgtccgtc actcaacgtg
    23641 gagatgaaga gccgctaaaa gcgaaacgga taatggaacg catacgttat ttgacaaaag
    23701 aaatcgaaat gactggtcgc aaccgacgca atggttgaac agttgacaag tccaactgac
    23761 atttgaccta gtagtaaaag caggtcagcg aatcattata tggacagaaa tgaccggaga
    23821 caatcaccgc aacaagtaat gcagaaactt aaacggagga ttttttatga gggctatttt
    23881 ttctagtata tctgtagttg gtctattttt tcttttagca ggctgcggta gcgcagatag
    23941 caacacaggt tctgttgatt cgattgatgc gggtcaaaca gtaaaaacaa gcagtacgaa
    24001 aacggagaga caaagcatat ttgtcggaac gattgaaagt atcactcaag tagaagcaga
    24061 agaagaaacg cttcagttga aactcgtcga tgtcatggcc aaagaagatc cagcgaatat
    24121 gggaacgtca ttcagcaatg acggagtgat cttaaatgct actgctgaaa aaataagcgt
    24181 ggacataaat gaattgaaaa tcggtgacaa agtccaattt acattagttg agatgccgat
    24241 catgacgatg tctatcccac cgcaagttcc tggagattcg attattgatg tttcattgaa
    24301 caaataacca ataacatttt ttcaaaaata agaaaatgtt gttttataga acgaaaaggt
    24361 atatttttta aggagatatg ggaatttttt tattcctagt ctccttattt tatttggaaa
    24421 atgctataat tatctagaag gagtgaagct atgaaccatt tagatttatc tcagcgctta
    24481 gccgcagtgg gtgactttgt ccctgaatcc gctcgtttag cagacattgg atcagatcac
    24541 gcttacttac ccgttgcatt gatattaaga ggaaaaatcg aatatgcagt tgctggtgaa
    24601 gtcgttcaag gtccttatga atcagcacgt aagcaagtca aaaaagatgg attagaagac
    24661 cgtatcaccg tacgccttgc aaatggtcta gatgctatcc agccacaaga tggtattaca
    24721 gctgtcacga ttgcgggtat gggaggttca ctgatccgag atatcctaga agctggcaag
    24781 ttgaatcaac gcttaagcgg acaagaacgt ttgatccttc agccaaacat cggagagcgt
    24841 acattacgat cttggctgca agaaaatcag tataaaatcc aatcagaaaa aatacttgaa
    24901 gaaaataaaa aaatatatga aatcattgta gccgaaaaag aacaaaaacc agttacttac
    24961 aatgaaatgg agctgatgtt cgggccattt ctgcttgaag agaaaagtga gatcttcaaa
    25021 tcaaaatgga caagagagct tcaacaaaga aaagctatcc gtgaacagct caaacaagca
    25081 gctaatcaag aggcacgtca aaaacaaatc gagcaggaaa tccaaatgat tgaggaggtt
    25141 ttaaagccat gacaatcagc ggacgcactt tcatccaaaa gtttgaaagc tattgtcctc
    25201 agtggctagc tgaagaagga gacccagtcg gactgcatat cggaacattg gataagccaa
    25261 tccaacgagt aatgatgaca ttagacgttc gtccagaagt ggtcgaagaa gcaatcaaga
    25321 aaaaaatcga tttgctgatt gctaaacatc ctccaatctt tcgtcctgta aaacggctgg
    25381 ttactgatca gccacaagaa aaaatgtatg cagatttgtt gaaacatgat attgccgtct
    25441 atgcagcaca cacgaacatg gatattattt gggatggact gaatgactgg ttttgtgaac
    25501 ttctcggtat tgaagtggag agttatttag taaagactca tgagattcat tataaaaaac
    25561 tggccgttta tgtacctgtg gatcatgcac agaaaatgcg tgaagtatta gctgcagctg
    25621 gtgctggtac acaaggtgat tataccggta caagttttac ctcgattgga cacggtcgat
    25681 ttactccgga agctggtgca caaccagcga ttgggaaagt agggaagaca gaacaagttc
    25741 aagaggcaaa agttgaggtt attttgccgg aaactatcga aaaacaagtg attcaagcca
    25801 tgcggtcagc tcatccttat gaggaacctg cttatgatct tttcgctata gatgaaccag
    25861 tcgaaatgtt tgggttagga cgagtaggtg aattgccgca agaaatttct atagaggcat
    25921 ttgtagaaca agtaaaagaa gcgttccaac tagatggact gagaatagtc cagccaaaaa
    25981 atgcaaaatc ctctgtgaag cgcattgcga tttgcggtgg aagcggtgaa aaattttatc
    26041 cgcaagcaat cgcccaaaga gcagatgtct atatcacggg agatatctat tatcatacgg
    26101 ctcatgatat gcaaagcgct gggttgattg caattgatcc cggtcattat attgaatcgt
    26161 tatgcaagca acgatttatc gagaaatttg aattttggaa acaagaagaa aattgggata
    26221 ttaacttttt cgtttctgaa acaaatacaa acccatttca atttaattaa aaggagagaa
    26281 tactatgtac gaaaatcttt tgccacgttt tttacgatat gtcaaaacag aaacacgctc
    26341 cgatgcaaca agcaaaacga ctccatcgac tcagacacaa gttgcttttg cgcaagaatt
    26401 gaaaaaagaa ttagaagagt taggcttagc tgatgttttc tataatgaag ataacggatt
    26461 cgttatggga acattaccaa gcaatgttga tcatgaagtg cgttccattg gatttatttc
    26521 ccacatggat acagcagatt ttaatgcagt aggagtcaat ccacaaatca ttgaaaacta
    26581 tgatggcgag tctacgatcg tactagatgc ggaagaacgc tatacattga atacaaaaga
    26641 ttttccaaat ttgaaaaatt atgaaggcca cacattgatc acaacagatg gcagtacatt
    26701 actgggagca gatgacaaat cagggattgc ggaaatcatg actgcaatgg agatcttgat
    26761 caataatcct tctatccctc acggtaccat caaagtggct tttggtcctg atgaagaaat
    26821 cggtgtcgga gcggataaat tcgatgttga gcaattcgat gtagattttg cttatacagt
    26881 cgatggtggt cctttaggtg agttgcagta tgaaacgttt agtgctgctc aagcaaatat
    26941 tacgatccaa gggaaaaatg tacatccagg gacggcaaaa gatacaatga tcaatgctct
    27001 acagttagca atcgattttc ataatcagct tccagccgat gaagttccag aaaaaacgga
    27061 aggatacgaa ggtttcttcc atttgatggc attggatggg aatcctgaag aagcttctat
    27121 gagttatatc attcgcgatc atgatcgtga aaaattcgaa gcacgcaaag cacaaatcac
    27181 acatatccaa gaagagttga atcagcgatt tgatcaagaa cgaatcaaag tagatatgta
    27241 tgatcaatat tacaacatga aagaaatcat tgaaaaagat atgagcatcg tggaattagc
    27301 gaaacaagca atgatcgagc ttggtatctc tccagtgatc gaaccagttc gtggaggaac
    27361 agatggttca aaaatctctt acatgggtat tccgacgcca aatctatttg ccggaggaga
    27421 aaatatgcac ggacgattcg aatttgtttc tttacaagta atggaaaaag caacagacgt
    27481 gattgtaaaa attgcagaat tgaacacaaa gagttaaaat cgaaaaagaa cagcttatga
    27541 caaggcgttc gtcccaatga tggaatgtca tctgtctaag gctaaagggc aggacaatgg
    27601 aattggttgt cccgccctct tttttgaaga gtaaatagga ggtaaagaat ggaaaagctg
    27661 ctagtttttt ataacaaaag ttcagggaaa gatgagggtg agaaattagc acgctggttt
    27721 caagattatg taaaagaaaa gcagccagaa ttatatgtag aactagctga aactggccca
    27781 tcgattgatg atgcggaatt agtcaagaaa gcagaagcca tagaagctga tactttagta
    27841 attataggtg gagacggcac gattcatcat atcgtccaag cgttccaaaa aacattatct
    27901 caataccatg tcggattact tccaggtgga actgtcaaca accttgcacg tgttcttgag
    27961 attcccctca aaaaagaaga agctgctgac gtgatcctgg aaggcaatgt acggaaaata
    28021 gactatggtc aagtcaatga tgaagtcatt atctctacac tgacaatcgg aatcttagca
    28081 gatacagcag tttggatcag tcaagaagaa aaacagcgtt acggctcgtg gatctttatc
    28141 aagaagtttt ttaagttatt gctgaagaag aaaaaatatc atttggattt agaaacagac
    28201 gaggagcatt ggaaaggaaa atcccaactg ttgacagtca cgatgtctaa ctccgtgggc
    28261 ggttttacaa attttgacga ctcagcgacc ctagatgatg gaaaatttca tgtaacgatc
    28321 gtaccttcct tagatatttt ccgatttatt ttttatttgc ctagaatcat gagaggcaaa
    28381 ttctatacga tccccggcat tacttatttc acagccaaac gcatgaaaat ccaagcgcag
    28441 gaaaaatctg tgggcactcg aacggatgga gacacgacag atgatcttcc agttgaatta
    28501 tctgttgtat caaaaggatt aacagtttat tcaccaaaga aagaagaaaa agcttagaaa
    28561 tagtagttaa cagaaagttg gaagaagagt aatggatgta ttgttacaca atcctttgtg
    28621 gttttacctt ttcattgtga atttttactt attttgtctg atggggtatg ataaacatca
    28681 ggctagaact agcggctggc gtgttcctga gaataatttg atatttgtca gcctgttagg
    28741 cggtggtttg ggaggattga tctctcgaaa agttttccat cataaaactc ggaaaaagaa
    28801 gtttactata ggattcgctg caggtgtact tgttgatttg attctcattt attttttcca
    28861 ttaaggagca ggagatgtcg tctaaaggaa cgaaaactaa aatcattctg aatctgcttt
    28921 tgctgattgt gatttttgcc attatttatt atttaatcaa tcaatcattt gcagatatct
    28981 ttgccgaatt gctgtctacc agtgttgttg ttttggcagc ggctgttata ttagggacag
    29041 tttaccaaat cgctgaaggg cgctcaatca aagaaattgc tgaacctttc gcgccgaatt
    29101 ttacaacgaa agatgggttc ttgacctcta gctatattgc gttttaccga atcgtttcgt
    29161 ttggtacagg aacgctgatc tcggagattt atttttataa caaaaaagga atgaagctat
    29221 cccagggagc aggggtgaca gcactccata tgattatgta caaactggca gtgatcactt
    29281 atgcattagc tggcttggtc atccaattct ctctttttta ttctaatggt ccatcaatga
    29341 tctggtttat catcgctggt atccttttga cgatcgtgat tgttgccttt ttattggcga
    29401 tgtccgtcag cttgaatgtt caaatatttt ttgtcagctt atcgaataag ttatttaaaa
    29461 gtgagcgttt gcggaaatgg gtggatacat gcaatcagca gatctactct ttaagggaaa
    29521 cggtccaaac cattatccaa gacaggactg cgctattgcg gatttatcta tggaatatgt
    29581 taaaacttgt attttggtat gtacttcctt atttgattct gattgaaaac catcctaaaa
    29641 tagattttct actgaccatt tcgtttatca gctttgcagt agtgctttca ggggttattc
    29701 ctacgcctgc tggtattggt tcctttgaat ttgtctattt gttattattc agaccagttg
    29761 ttggaacagt tgacgcagtt tcttcgcttt tactgtatcg tttcagttcc tttatcttgc
    29821 catttctcta tggatttgtg tatgtgattg ctgaacggcg aagagtgatc aaagaagaaa
    29881 taatagaagt aaaaaaagag aataaagaaa atcaagggta ggtcttttat aagatctacc
    29941 ctttttatca aatcaaagaa aaacgttatg gataagataa ttttacgttt gtttggctct
    30001 ttttttatat acatacaatc aattttgttt ttttaagatt tatataagtc gagaaaaaaa
    30061 gtcaaaaaac tatgtgtata ttttgaccaa aattgaccaa agcgatataa tagtttttgt
    30121 aaagatcaaa gaaataagtg atcttacctt taattaggag ggtatacctg tatgaatatt
    30181 gagaaaatga ctacgacgct tcaagaagcg attgctgaag ctcaacagat tgcggtcact
    30241 cgaaaacatc aagatatcga cattgcccat gtatggaaaa tctttttaca atctaatcat
    30301 tttggtcgaa atttctatac ggatgccgga ttggatgtcg aatcttttga acatgaaatt
    30361 gatcgtctac tggatgagta tccagttgtt tctggtggaa acgtccaata tggacaaaac
    30421 ctcagccaga atttctttcg attattgaat gaagcagacc aaatacgtga aagcttcggc
    30481 gacgagtttc tttcaacgga agtcgttatt ttagcgttga tgaaattaaa aaattatccg
    30541 ttaactgttt atttgaataa aaacggatta tctgaaaaag aattgcgtaa aaacatcgaa
    30601 gagatgagag gaggagaaag agtgacctca caaaaccaag aagaacaata caaagcatta
    30661 gaaaaatacg gcgttgattt agtacagcaa gtcaaaagcg gaaaaatgga tccaatcatc
    30721 ggtcgtgatg aagaaatccg tgatgtcatc cgtattcttt cacgaaaaac aaaaaataat
    30781 cctgttctga taggagaacc cggtgtaggg aaaacggcaa tcgttgaagg gcttgctcaa
    30841 cgtatcgtac gcaaagatgt gcctgaaaat ttaaaagata aaacgatttt ttcattagat
    30901 atgggtgcat tgatcgctgg agcaaaattc cgtggtgaat ttgaagaaag actaaaagca
    30961 gtcttgaaag aagtcaaaaa aagtgatgga cgtatcctct tgttcatcga tgaaatccac
    31021 aatatcgtag gggcaggaaa aacagaagga agtatggatg ccggaaatct gttgaaacca
    31081 atgcttgctc gcggtgaatt acatttgatc ggtgccacaa ctttagatga gtaccgtcaa
    31141 tatatggaaa aagacaaagc gttggaacgt cgctttcaaa aggtattagt caaagaacca
    31201 acagtggaag atacgatcag tattctgcgt ggattaaaag aacgttttga gatccaccat
    31261 ggtgttaaca tccatgacaa cgcattagta gcagcagcta ctttatctga ccgttatatt
    31321 actgatcggt tcttaccgga caaagcaatc gatttgatcg atgaagccag tgcaacgatt
    31381 cgggtagaaa tgaactctat gccaacggaa ttagatcaag ttactagacg tttgatgcag
    31441 ttggaaatcg aagaagctgc actgaaaaaa gaatcagatg atgcaagtaa aaaacgcttg
    31501 aaaaatttgc aagaagaatt agctgaactt cgtgaagaag caaatgcgat gaagatgcag
    31561 tgggaaacag aaaaagaaga agtcaattct gtttcagcaa aacgtgctga aatcgataaa
    31621 gcaaaacacg aattagaaga tgcagaaaat aactatgatt tagaacgcgc tgctgtattg
    31681 cgtcatggta cgatacctca attagaaaaa gaattgaagg aattagaagc taaagcaaaa
    31741 gacagcgaga ttaagatggt tcaagagtcc gttaccgaaa atgagattgc tcaagtcgtt
    31801 ggcagattaa cgggtatccc cgtaacgaaa cttgtagaag gtgaacgaga aaaactaatt
    31861 aaattgaatg aaacacttca taaacgagtg attggtcaag atgaagcagt tgatgcggtc
    31921 agtgacgcgg taattcgttc acgggctgga ttgcaagacc caaatcgtcc tctaggttct
    31981 ttccttttcc taggaccaac tggtgtcgga aaaaccgaat tagcgaaagc gttagctgag
    32041 aacttgtttg actcagaaga tcatatggta cggatcgata tgagtgaata tatggaaaaa
    32101 catgctgtgt ctcgtctagt cggagctcct ccaggctatg taggttacga agaaggtggt
    32161 caattaacgg aagctgtcag acgaaatcct tatacaatcg tcttgttgga tgaaatcgaa
    32221 aaagcgcatc ccgatgtctt caatatcttg ttacaagtcc tagatgacgg acgcttgact
    32281 gattcaaaag ggcgagtagt agatttcaaa aatacggttc tgatcatgac cagcaatatc
    32341 ggttcacagt tactgctgga aggcgtgact gctgatggaa cgattccaga agcagtggct
    32401 gaacaagtca acacgctatt acgtggaaac ttcaaaccag aattcttgaa ccgaatcgac
    32461 gacacgattt tatttacacc attgagccta gacaacgtga aaggaatcgt tgacaaaatg
    32521 gtggcgcaat tggcacaacg tctggaacat caagaaatcc tgctgacaat cagtgatgaa
    32581 gccaaaacgt ggattgctga aaatgcttat gaaccagcat atggtgcaag accattgaaa
    32641 cgatttatca ctaaggaagt agaaacacca ctagctaaag aaatcgttgc aggacatgtt
    32701 atgcctaaat caaaagtaac gattacttta cttgatggac aacttcattt caaaacagaa
    32761 gagctagaag aaatcgtcta atcttccatt gagactataa aagaaaaaag gggtctgaca
    32821 caactagtgt cagacctcct tttttcaaat aaacagtgtt ccagaagtag tttcttcgaa
    32881 aaataagccg aaatttcttg aagctgcccg cttctgtcac aacctctttt attaggtatt
    32941 tttcactttt tctaatttat aactttcaaa gtaacgctca tcttttttac tttgagacca
    33001 tgcaaacaat cccattaagg cagcacctat cgtatttgtc agcaagtcgc ccatcgtatc
    33061 catcaatgct gctcgtccga caaacagtgt gccatcagaa gcagcaaaac gttgaagatt
    33121 catgcctaag aactggtcgc ataagaattc ccagaactcc caaaacacgc cgcaaagtcc
    33181 agcaaacgca aagccgaata agaggaacaa ccaaggactg gtttttgata tctcagcatc
    33241 tttcatcaag taaccaatca atccatagcc aagagctgtc aggaccattg gactcaccgc
    33301 atgtaagatt ttgtcccaaa aagagatgat actgatcaaa tgcatccctg tgcctaaaaa
    33361 gacagagatg aacaagaaaa accagtaaaa caaaacgatt gctgctggta ttttaagttt
    33421 gaaaactttc aaaatgactt gtggcatgaa aatcaatgca atccctaata aagattccac
    33481 gataaatgtg attccttgag aagtcgaata tttttgtgtg ataaattcat aaaaattata
    33541 gcagacaacg aatacaccaa aagcaagtaa agctatttgc acctttttga tttctttaat
    33601 cactgaaagt ccctccatct aaaaatcttt ttacttctga aagcaactca atttctgaag
    33661 aaaccacttt tccgttcaat ttgatcaaac ctattgtata taagttcagg tagtgaaatt
    33721 gattttcagc tgtttcttgt agtgcagcga tttttttcgg gtgggttccg ccttgctgtc
    33781 gagtatcagt atataagccg atcactggga tgcctttcgc ataagccaca ccgatttccg
    33841 atgctacccc ggcatcgatc gtcagcccat ctagtaaagc aatcattaaa tcactagcca
    33901 acactttttc tgtgtcagca agagcaatca ttcgactgtc tgcataagca gttttgtcat
    33961 tgattgcttc attttcttga ggaagataga tcgaaagctc agaagagagc tctctgattt
    34021 ctttaactaa ctgttggtta tataacaaat cacttttagc aaacattggt gcggcaaaat
    34081 aaattttcat ctaaacatca tcctttttat caaaaataat cgctaaagta agcttccatg
    34141 tttggaatga tacgctctag tgacttcagt gtttcttcat ctgtttctaa ccgttcgatt
    34201 cttgccaaat aagaagcacg gcgataattc atcatcaagc aagtcaatgt ttggatatcc
    34261 agtcgaacgg attttcctat aggttcattt aagacacgga cttgtccctg ttcatcccat
    34321 gttaaagcga agacaccatt attccaagga gcgacaggat cttcaatgat gaaatgaaaa
    34381 gcatcagctg ttccaacaaa cggaaaccgt tgaaggaatt cttttacatc aacgatccga
    34441 gccataaaat atggttcgat ctgctctttg atctgactat cttcaatcaa aaatgctaaa
    34501 ggctcatttt tataaatatc tccatgtacc cagtagatca tggaaaaatg tgcggaaata
    34561 aagttccata agccatttcg agcttcttga tcaagataga acatttcttt tacgtgaaat
    34621 atttcttcag ctacccagta gaacagaaca cctcttggtt catggttgct atcgtagtag
    34681 acagcggctg tgcgctcatc ttcattttcg aaacgccaat actcttccca atgaaaatca
    34741 ctgcggaata aagcaccatg gttttgttgg gcaaatttgt cataaacgga aaatacgtct
    34801 ggatgatcaa ctggtaatcg ctcaaccata ccacttactg ggatttgttt aggtagctgt
    34861 gtatctctga ttttaaagga aagtttatca gacatgattt cccatccttt acgccgatag
    34921 taggggatac tgtaaggata caaataagag atccattgct tattcttgcg catattatcc
    34981 aatgcaacga taattagatc ttgcatcaat ccatggttag catattctgg ataagttcca
    35041 actccagtca caccacccat tttataacgt gcaccgtgaa tattgacttc acaaggatag
    35101 atagcgattt gagagatcag tttattttcg tggaaccatc cgaacacttt cgatacttcc
    35161 aaaatcggtt ttttggaacg gataaaagct cgtttattct caaacccgct ttcctcgatg
    35221 tctgcttcag tcacttggaa cacataggaa agcaattcat tgaattgatc gatatgttct
    35281 tgagtgaccg gttttaattc aagattttct ctaaaatctt tttgatccat tttttcatct
    35341 ccatttatta gcttgataac agtatagcaa aattctacaa aaaaaacttc tatagctgac
    35401 taattaaaaa ctctttaaat tactgctttt cgttggtata atggaaagtg ctgattatat
    35461 gaaaagagga gacgctatgg atataaagaa aatgaaagaa cgccaagagc agattcgtaa
    35521 tttttcgatc atcgctcata tcgaccatgg gaaatctaca ttggccgatc gtattttaga
    35581 aatgacgcac actgtgacgt ctcgcgaaat gcaagatcag ctgttagact cgatggattt
    35641 ggaacgtgaa cgtggaatca cgattaaatt aaatgctgta gaactcaatt acacagcgaa
    35701 aaatggagaa acatatatct ttcacttgat tgacacccca gggcacgtcg acttcaccta
    35761 tgaagtctct cgaagccttg ctgcctgtga gggtgccgtt ttagtggtcg atgcagcaca
    35821 aggaatcgaa gcacaaacat tggccaatgt ttatttagcg ttagacaatg atttggagat
    35881 cctgccagtc atcaataaaa tcgacctacc cgcagcagat cccgaacgtg tccgtcaaga
    35941 aatcgaagac gtaatcggaa tcgatgctag tgaagctgta ttagccagtg ccaaatcagg
    36001 gattgggatt gaagatattc tcgagcagat cgtggaatat gttcctgcac caagtggaga
    36061 cttagatgca ccgctaaaag ctttgatctt tgactctatt tatgatagtt accgcggtgt
    36121 tgttctaaat gtccggatca cggacggtat ggtcaaaccc ggagacaaaa tcaaactgat
    36181 gagtaatggc aaaacatttg atgttacaga ggtcggggtc ttctcgccaa aagcagttgc
    36241 tagagacttt ttgatggtcg gtgatgtcgg ttatatcaca gcaagtatca aaactgtcca
    36301 agatacacga gtaggggata cagtaacgtt agcagacaat cctgccgctg aagctttacc
    36361 aggttaccga aaaatgaatc cgatggttta ttgtggtctt tatccaattg atacttctcg
    36421 ttacaacgat ttgcgggaag ctttagaaaa actgcaattg aatgatgctg ctttacaatt
    36481 cgaaccagaa acctctcaag cattaggttt tgggttccgc tgtggattcc tcggacttct
    36541 tcatatggat gtcgtccaag aacgcttgga acgggaattc aatctagaat tgatcacgac
    36601 tgcaccatcc gttatttacc atgttaataa aacggatggt acgactgcta cagtagataa
    36661 cccggcagat tttcctgaac ctgtaacgat tcaggatgta gaagaaccat tcgtcaaagc
    36721 acagatcatg gtcccaaatg attttgtcgg tgcagtaatg gaactttctc aacgaaaacg
    36781 gggagaattc attacgatgg attatttaga tgattaccga gtaaatgtcg tctataatat
    36841 tccgctttct gaaattgtct ttgacttttt cgacaaattg aaatcaagca cgaaaggcta
    36901 tgcctcattg gattatgaaa tgtctggtta tcaaaaaagc aagttagtga agatggacat
    36961 cttgctgaat ggagaaaaag tcgatgcatt aagctttatc gttcaccgag actttgccta
    37021 tgaacgtgga aaagcaatcg ttgaaaaact gaaaaaacta atcccacgcc aacaatttga
    37081 ggtgccaatc caagcagcta ttggacaaaa aatcgttgcc cgttcagaca tcaaagcctt
    37141 gcgtaaaaac gtcttggcta aatgttatgg tggagatgtt tctcgtaaac gtaaactctt
    37201 agagaaacaa aaagaaggga agaagcggat gaaacaaatc ggctccgtcg aagttccgca
    37261 agaagccttc atggccgttc tgaaaatgga cgaggacgaa ccgaagaaat agcaagggtt
    37321 tgtttgaaaa cagtgtaaaa aataaaccat gtttacatgg atttgccacc ggtttgccac
    37381 cggtg
//
LOCUS       DANKEF010000023        36567 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000023 DANKEF010000000
VERSION     DANKEF010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36567
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.023"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(25..1521)
                     /gene="lysS"
                     /locus_tag="PWZ48_001322"
     CDS             complement(25..1521)
                     /gene="lysS"
                     /locus_tag="PWZ48_001322"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDL2260990.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKTGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="PWZ48_001323"
     CDS             complement(1632..2636)
                     /gene="dusB"
                     /locus_tag="PWZ48_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDL2260991.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="PWZ48_001324"
     CDS             complement(2637..3524)
                     /gene="hslO"
                     /locus_tag="PWZ48_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDL2260992.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="PWZ48_001325"
     CDS             complement(3741..5852)
                     /gene="ftsH"
                     /locus_tag="PWZ48_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDL2260993.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="PWZ48_001326"
     CDS             complement(5942..6487)
                     /gene="hpt"
                     /locus_tag="PWZ48_001326"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDL2260994.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="PWZ48_001327"
     CDS             complement(6487..7932)
                     /gene="tilS"
                     /locus_tag="PWZ48_001327"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDL2260995.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8052..8522)
                     /locus_tag="PWZ48_001328"
     CDS             complement(8052..8522)
                     /locus_tag="PWZ48_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HDL2260996.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8568..8993)
                     /locus_tag="PWZ48_001329"
     CDS             complement(8568..8993)
                     /locus_tag="PWZ48_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HDL2260997.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9060..9329)
                     /locus_tag="PWZ48_001330"
     CDS             complement(9060..9329)
                     /locus_tag="PWZ48_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HDL2260998.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9340..10935)
                     /locus_tag="PWZ48_001331"
     CDS             complement(9340..10935)
                     /locus_tag="PWZ48_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2260999.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="PWZ48_001332"
     CDS             complement(10950..14471)
                     /gene="mfd"
                     /locus_tag="PWZ48_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDL2261000.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14488..15048)
                     /locus_tag="PWZ48_001333"
     CDS             complement(14488..15048)
                     /locus_tag="PWZ48_001333"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDL2261001.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15401..16375
                     /locus_tag="PWZ48_001334"
     CDS             15401..16375
                     /locus_tag="PWZ48_001334"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2261002.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16545..17945
                     /locus_tag="PWZ48_001335"
     CDS             16545..17945
                     /locus_tag="PWZ48_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HDL2261003.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18023..18745)
                     /locus_tag="PWZ48_001336"
     CDS             complement(18023..18745)
                     /locus_tag="PWZ48_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2261004.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18824..19330)
                     /locus_tag="PWZ48_001337"
     CDS             complement(18824..19330)
                     /locus_tag="PWZ48_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2261005.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSIDHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19453..21252
                     /locus_tag="PWZ48_001338"
     CDS             19453..21252
                     /locus_tag="PWZ48_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HDL2261006.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21320..22894)
                     /locus_tag="PWZ48_001339"
     CDS             complement(21320..22894)
                     /locus_tag="PWZ48_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2261007.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="PWZ48_001340"
     CDS             complement(23149..23658)
                     /gene="mreD"
                     /locus_tag="PWZ48_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDL2261008.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="PWZ48_001341"
     CDS             complement(23662..24516)
                     /gene="mreC"
                     /locus_tag="PWZ48_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDL2261009.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24671..25309)
                     /locus_tag="PWZ48_001342"
     CDS             complement(24671..25309)
                     /locus_tag="PWZ48_001342"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HDL2261010.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25310..25960)
                     /locus_tag="PWZ48_001343"
     CDS             complement(25310..25960)
                     /locus_tag="PWZ48_001343"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2261011.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGQFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="PWZ48_001344"
     CDS             complement(25973..26872)
                     /gene="rsgA"
                     /locus_tag="PWZ48_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HDL2261012.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="PWZ48_001345"
     CDS             complement(27037..29106)
                     /gene="pknB"
                     /locus_tag="PWZ48_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HDL2261013.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29103..29843)
                     /locus_tag="PWZ48_001346"
     CDS             complement(29103..29843)
                     /locus_tag="PWZ48_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HDL2261014.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="PWZ48_001347"
     CDS             complement(29856..31214)
                     /gene="rsmB"
                     /locus_tag="PWZ48_001347"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDL2261015.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKTLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31214..32152)
                     /locus_tag="PWZ48_001348"
     CDS             complement(31214..32152)
                     /locus_tag="PWZ48_001348"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDL2261016.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKEAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32145..32636)
                     /gene="def"
                     /locus_tag="PWZ48_001349"
     CDS             complement(32145..32636)
                     /gene="def"
                     /locus_tag="PWZ48_001349"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2261017.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32651..35059)
                     /gene="priA"
                     /locus_tag="PWZ48_001350"
     CDS             complement(32651..35059)
                     /gene="priA"
                     /locus_tag="PWZ48_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDL2261018.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35312..35617)
                     /locus_tag="PWZ48_001351"
     CDS             complement(35312..35617)
                     /locus_tag="PWZ48_001351"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDL2261019.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="PWZ48_001352"
     CDS             complement(35622..36236)
                     /gene="gmk"
                     /locus_tag="PWZ48_001352"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDL2261020.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYINKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEERMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
BASE COUNT         9885 a         7988 c         6706 g        11988 t
ORIGIN      
        1 attccttaga cagcttttat tcgattatct cattgttggg aatagcaaaa catcccgaat
       61 tgattgtgca tcagttagca acattactaa tctgtcgatc ccgatgccta gaccaccagt
      121 tggcggcata ccgtattcta aggcttccaa gaagtcttcg tctacaccgt gagcttcgtc
      181 atttcctagt tcacgttctt tttcttgcgc ttcgaatctt tctctttgat caattggatc
      241 gttcagttct gtaaaggcat tagcaaattc gcgccccact atgaatagtt cgaatcgatc
      301 tgtgaatcga ggatcttctg gatttttctt cgctaaaggc gaaacttcga ctggatgtcc
      361 gtatacaaac gtcggttgtg tcaatgtttc ttcaacaaat gtttcaaaga attcattgat
      421 aacatgtccg acacccatag cttcggttac ttctacattg tgtttttcgg ctaaagctaa
      481 tgcttcttcc aatgtcattt cttgccagaa gtctacacca gtttgttctt tgattgcatc
      541 taccatatgg atgcgtttaa agtcactttc taagtcgaca gcttgtccat catatgtgat
      601 ttttgctgtt cctagtactt tttcagctgc gttacggata ataccttctg tcagattcat
      661 tacatcattg aaatcagtat atgctgtgta tgcttctaac atcgtaaatt ctggattatg
      721 tgtagtatca attccttcat tacggaaaac gcggccgatt tcataaactt tttccattcc
      781 gccaacaatt agtcgtttta aatgaagttc tagcgcaata cgcaaatata aatcaatgtc
      841 cagtgcatta tgatgcgtga taaatgggcg tgcggcagct ccacctgcct cattgtgcaa
      901 tactggtgtt tctacttcaa tatagccatt gccatctaaa tagcgacgga tttcgctgat
      961 aatttggctt cgtttcataa atcggtcaaa gctttcgcga ttactgatta aatccaagta
     1021 gcgttgtctg tagcgttgtt caatattcgt taagccatgg tatttatctg gcagtggacg
     1081 aagtgctttt gacaaaatcg tgatttctga agctttgata gaaacttcgc ctgtgtctgt
     1141 tttcatgatt tgtccagtta caccaaagaa atctccaagg tctgcatgtt tgaatacttc
     1201 ataggcctca tcacctactt ggtcttttct tacataaatt tgaatttggc cttcacgatc
     1261 ttgtagatgc gcaaatccag ttttcccttt tcctcggcga gtcatcatac gaccagctac
     1321 actagcggtc aatcccattt ctgctagttc ttcttttgtt cttggatcaa atagttcatg
     1381 caattcttct gagttgtgcg tgcgttcaaa tcgttttcca aatggatcga ttccatcttc
     1441 acgcaattgt tccatttttt gacgacgtac aagcatttga tcatttaaat cttctacttg
     1501 atgttgttgt tcctgtgtca caaaagttcc tccattctca tgttaatctc tttcctataa
     1561 tgccaatgaa acacaaaaaa agcaagccga acttggaatt tcggcttgaa aattcacgaa
     1621 aaacttagaa atcacaacgt ttgtttcaat agtacttttt ccgctttttc tacaaattga
     1681 tccagcaggt cattgatttc ttgctgtttt tctgtctgat tgattgctac tttcactttc
     1741 gctgctcgag gaatcccttt taaataataa gccgcatgtg ttctgaattc tctacaagct
     1801 actttttctc ctttgagatc tgctaagcgt tgtaaatgaa gtttagctgt agcaattttt
     1861 tcggctggtg ttggttcagg aatcagttct cctgtttcga gataatgctg tgtgcggtga
     1921 atcatccatg gattgcccag cgctgcacga ccaatcatca caccgtctgc accgacatag
     1981 tcaagcatcc attttgcatc ttctggcgtt ttgacatccc cgttccccat aaatgggatc
     2041 gtcaaatgtt ttttcacttc tttcaatact tcccagtccg cttttccttc atacatctga
     2101 acccgtgtac gtccatgcat agcgatggca cttgctccag ctgattgtgc tgctaatgca
     2161 ttttctacag caaagacgtg ctcttcatcc caaccaatac gcatcttgat tgttactggg
     2221 atatttactg ctgaagaaac agcttggacc atttcgtata ctttctcagg atctaaaagc
     2281 cattttgctc ctgcttctgc tttgattact ttatttactg gacagcccat attgatatct
     2341 attatatcgg cagctgtgtt ttcttcgaca aattgagcag cttcaactag actatcttta
     2401 tttccgccaa atatctgtaa acttaatgga tgttctgttt cttcaatgtg gagcatctct
     2461 aaagtttttt tgtttctgaa atgaatacct tggtcactga tcatttcaca tacgactaag
     2521 cccgcaccaa attctttgac agtgactcga aatgctgaat ttgttattcc agccatcggt
     2581 gcaacaacga cacgattagg gatttccaca ttgccaattt tccatgtgtg ttccacttaa
     2641 atccctccta aggcttcttt cttcaatcca tctaattctt cttcgctata cttgtatttg
     2701 ttattacaga aagaacaaac cgcttctgca ccgtgatctt cgtctatcat tgcttggatt
     2761 tcttctgcac ctaacgcgat aattgcttct gcaaattttt cttttgaaca gtcacatttg
     2821 aattgaacag gcattttttc caaaatgttc aattcttctg taccaagcaa tcctttcaat
     2881 atttcttctg gtgtctgtcc ttcatctaag agggtagaga ttcttggtgt cgtttttaat
     2941 tgttcttcga tcttgctgat tgtttcttca tcggcacctg gcatgatttg gatcatgaat
     3001 cctcccgccg tcttgatgct gtcgtcagta ttgaccaata cactcagtcc aacagctgaa
     3061 gggatttgtt ctgagatagc aagatagtaa gtaaaatctt cagcgatttc tccagaaaca
     3121 atcggcgttt gcccagaaaa aggttctttt aatcctaagt cttttgtgac agtaaacatt
     3181 ccttgtgttc caactgctcc ccgcacatcg attttacctt cactatttag aggaagactg
     3241 agatgcggat ttttgatgta acctttcaca tctccctttc catttgcatc tactacaatt
     3301 cctgttgctg gaccatcgcc ttggattcga acagttagtt tgtcttctcc tttgagcgtc
     3361 gcaccaagta gcagactacc gaccaatgtc cgtcccaaag cagctgagga ggtgtgccaa
     3421 gtatcatgac gtctttgtgc ctccgctacg gtatttgttg cttgaacggc gtatgctcgg
     3481 acgaatccgt cgtgagcaag tgccttaacc aaataatctt tcatttgtta tgtactcctt
     3541 tttccgtttt taaactcata gtgaatcata ctagcaacta ctgctttttt caactttgga
     3601 atgcttcttt taaaaataat tcagaatcat ctagaaaaca ggacatcacc ctatttacaa
     3661 cttaaataaa gaaatctccc gaaaaccggt ttttgttccg gttttcggga gtcatatcag
     3721 atcaattgac ctatattttt ttatttgtat tgatcgtctg aatgattatc ttctgattgt
     3781 tgagaatctg aatcatgttc tgctttattt tcctcattca gttcatgttt tgcttcttca
     3841 aaatcacgtt tttcttccgc ttgtttttca gcgtcttttt cttccaaagc acgttttgct
     3901 tcttcaaaag ttttcgcttc tttttcgctt ggataatcat ttgactcagt accttcaggc
     3961 atcacaccgt gttcaaacaa tgacttgatt gctttggcat ccaatgtttc gaattccaaa
     4021 agtttttcag caatcagttt atgctgtgca cgatgagctt cgatgatttc tcgagcttta
     4081 tcatgagctt ccatcaagat ttttcgtact tcttgatcaa tctcgaaagc gacttgttcc
     4141 gagtaagctt ttgtttgacc atagtcgcgg ccaacaaaca cttgatggtt tccttcatat
     4201 tgaacaggac ctaaacgatc actcattcca tattctgtta ccatgcttcg tgctagagct
     4261 gtcgcttgct caaagtcatt ggaagcacca gttgattgga cattgaagat gatttcttca
     4321 gctgtacgtc caccaagtaa gcctacgatt tgttcaaaca tatcttcttt cgtcatcaag
     4381 aactgatctt cttttggtaa agcgatcata tatccgccgg cgcgtccgcg agggatgatc
     4441 gttactttgt ggacgacccg agcgcgactc aatactaatc cgacaatcgt atgaccagct
     4501 tcgtgataag caaccatttc gcgttctttt ttgctgatta cgcgatcttt tttagctgga
     4561 cctgcaatca cgcgatcttc cgcttcatcg atatcagaag catcgatttt ctttttattt
     4621 cttctagcag ctactaacgc tgcttcattc aagacatttt ctaaatctgc tccggcaaaa
     4681 cctggtgtct gttgagccac aactttcaag tcaacgtcat ctgccatcgg tttattccgt
     4741 gcatggactc gcaagattgc ttcacgacct tttacatctg gacgtccaac taaaatctgg
     4801 cggtcaaaac ggcctggacg tagtaacgct gggtctagta catcagaacg gttggttgca
     4861 gcaatgacga ttactccttc atttccgtcg aagccatcca tttctacaag caattggttc
     4921 aatgtttgtt cgcgttcatc atgtccgccg cccataccgg caccacgttg acgacctacc
     4981 gcatcaattt catcaatgaa aatgatagct ggtgcgtttt tcttcgctgt ttcaaacaaa
     5041 tcacgaacac gacttgctcc aacaccgaca aacatttcta cgaaatcaga acccgagatc
     5101 gagtaaaacg gtactccagc ttctccggca acagcttttg ctaataaggt tttacctgtt
     5161 cctggaggtc cttctaaaag tacacctgcc gggatacggg cacctaattc aacaaatcgg
     5221 cgaggatctt tcagaaattc aacaacttca actagttctt gtttttcttc ttccgcaccc
     5281 gctacatcag aaaaacgtac gcgattagct tttttgtctg cttctttggc ttttgattta
     5341 ccaaagttca tgacacggcc gccaccgcca ccgccgccgc cttgttgtcc catcatcatg
     5401 taaaagaaaa agatgatgat tacgattggc aagaagctaa gcaataggga aacccatata
     5461 ccgctgcttg attcttcttt tactgttgta gcaactttgt tttcttttgc cagttctgtt
     5521 acttggctca atgttgtatc atttggcaaa atgattgttg tgaaatgggt agtagtcgta
     5581 tctgttgaac ctaagatagc taacccaccg ctgttagcga cggtctgctg ttctttgtat
     5641 tcaccagtga tcttatatac accatttgca ggctgcactt caaattcttt gatcttgcct
     5701 gcttgtagtt gttctgtgaa ttttgagtac tcgatatctg acgatcgctg gccgttatta
     5761 ccaaaaatga aataaacgac cattaccatc gctaagatta cgaggacata atacaaggca
     5821 tttttcatca tgccgttgtt ctttttgttc atagttgccc tccctaagcg tcaatttaat
     5881 ttaacagttt tttatcaatc ctgaccattc tattgtacca taatttaatt gagatacagc
     5941 attaatttga ttgataaaca ctaggtttca atacaccgat gtacggaagg ttacgatatg
     6001 cttctgcata atccaagcca taaccgacca caaattcatt tggtacgttg aagccaacat
     6061 aatctgcttc gatatttact actcttcctt caggtttgtc aagtagtgta acaattttga
     6121 ctgattttgc tttgcggtat ttgaaaagat ccactaaata agcaagtgtt cttccgctgt
     6181 caatgatatc ttcaacgatc aacagatcgc gaccttctac attcgtatct aagtctttga
     6241 cgatttttac ttcccctgac gagactgttg cattcccgta gcttgaaaca tccatgaaat
     6301 ccaattccaa atacgtatcg atagaacgaa cgatatctgc catgaatggt accgctcctt
     6361 ttaagacacc tactactagg ggattggtgt tttggtaaat ttctgttaac tctttgccta
     6421 gttctttgca acgaacttgt atctcttctt gtgagatcaa aattctctca atatcctttt
     6481 ccaacatcag gttctccttc caattgcttc tttttgatat ttgtaaagaa gtatgtagtg
     6541 tattttatca gtttcttcac gaatactcaa atatgaaaag gcaaaaggta tcaagctata
     6601 tatatttctt ttggcatctg taatgatcca agcatgctgc ctttgtgaat taggtacttt
     6661 ctgatcgata aaatatctag atactttttt tgtgagattc tctgttaatt taatgcggtc
     6721 tcctgctttt cttttttcga catacagttg cacatcagca ggaatccaaa tgtcatgaga
     6781 aaatagagac catgaatctg ttgctttgat gtcccctgca ttttcaggtt tgaccagacc
     6841 aatccattct gtttcattca aatatattcc ttgttctgga atcagcaaca agcgctggtc
     6901 ttctgtctgg ctagttgctt gtttttcttt tttagagagt acaacaaaat gatactctct
     6961 tttcagtatc caattattgc caatactgag ttgccattgg ctttttttct gttgccactg
     7021 gtctaaaatc gactccattt gttgttcttt caaaataatt cctgtttttg agaatgcttt
     7081 gtcgaaaaag gagtaaaaaa aatagtatcg ttcagcatct gtcatttctt caatcatttc
     7141 tgcagaaaat cgaaattgtt cctcccactg ttcgacttcc ttttcgatca actgtcccat
     7201 atatttttga atgacttggt ctgcccattg gatctgttgc gaaaattgct ggaaatgtct
     7261 caacgcctgt gtattttctt gtttcaattg aggaactacc aaatgacgaa gccgatttct
     7321 ctggatatct aacaattggt ttgtctgatc ctcaaagtaa acaagctgcg attccgctgc
     7381 ataagtatat aggtcttctt tgctaaaact cagcaacgga cgaatcagtc tgcccgtagc
     7441 aaatggttgt acttctttga ttccagaaaa agtatttaac tgtcccccac gaacgatttt
     7501 cattaacacc gtctcgattt gatcatctcc gtgatgagca gtcatcaacg tgtcatattg
     7561 tccttcttcc atcacgcgag tgaattcttg ataacgaaag ttacgagctt ctgtttctat
     7621 ggcggatttg gctggatctt cccaaatact tacatataaa gggagattct tctcttcaca
     7681 atatgagcga agataggcag cttcttctgc tgattcttct cttaattggt gattgatgtg
     7741 gacgatacct aaagaaaaac cgatttggcg ttgcacttgt aaaagtaaat ggagcaaaac
     7801 catcgaatca acaccacctg ataccgctgc taaaattctc gatccttgtt gccagaatcc
     7861 ttgttcattc ccgtgccgaa taaatgactg ctgtaatagc ggtgcttttt tcccgccttt
     7921 ttcaacgttc atcctttcca caccctttct tcctaaaaaa aagagagcgg gaaaacgtct
     7981 aaaacgacaa ttaacctgcc ctctgttgat actatgaccc aaatagtcgc actatcatta
     8041 tttgcggctt tttagctgcg acgtccgcca cggccaccgc gctttccttc tgtgtttcgc
     8101 ttcaatgaag ataagcggtc atcgctatct ttcaaaaatg aactcatcaa tgaatcgaag
     8161 tcttcttttt gagctgaagg ttgactgcga gaaaaagatt ttttctgtgc tggacgattt
     8221 cggttgtcac ggggatcttt ttgttgatat tttttaggac ggctttcttc tccagattca
     8281 gcaggttgtt cctgcgcttt ccggatagat agtccgattt ttccatcatc atttactgaa
     8341 ataactttaa ctgttacttc atctcctaca cttaaaacat catggatgtc ttttacatag
     8401 ccatttgaca cttcgctaat atgaactaga cccgtttttc cagcacctag atcaataaac
     8461 gcaccaaaat ttgtgattcc tgatactttt ccttgcagtt ttgctcctac ctcgattgac
     8521 ataaaaaaaa tgttcctcct aattatttcc cttttttata ttcctgctta ttttgcagtt
     8581 gtagaactag ttgattgatc tgtttcattt gtcgagctgt ctgtatttga ttctaatata
     8641 ggataaactt tttcaccgtc ttttgagtaa aagaatttgc ttctggcaag tttagctaca
     8701 tactcatcat ctttcagcaa tgaaacatct tggtgcagct gatcaacgtc tgcgttgact
     8761 tctttcattt cagtatttgc ttcgactctc aattcgttca attgatgcat cctttgcatg
     8821 tcatggaaaa tctgcacacc tattatcgcg aaaatgacca aagcaccaac aaaaattgca
     8881 gccagtctgc gtcttcgaaa aatcagttga cgttgttgtt gctgaaattc agcatacttt
     8941 ttctttgcat attccgtatc tagcgcagcg attttattgc tttttttcgt cacttggatt
     9001 cgctccaatc tttttgattg tctttttcat tataccaata tcgtgaaagc ttgtctaatc
     9061 tatttctcta gtgtttctgt gcgattttca gaaataattt cgtacatttt ttgtgcatct
     9121 tcttttttgg ttgattcatg taattccgta attttcactt ccaacgtttt gttgccaaac
     9181 tggactttca aaatatcgcc tactttgaca tcactggaag atttcgccaa tttaccgttg
     9241 atttgaattc ttcctttatc agctacttct tttgctactg atcgtctttt gatgattcgg
     9301 gaaattttta aaaatttatc taatcgcata tcttattcct cactttttaa tttttttatt
     9361 taatcgcagg attttcttac caaaaggcaa cattagccat tctcgtactg taaacagacg
     9421 aagacgaatc actgcataaa tgaacacaga agccccgatc ccaactccta tcacgctgac
     9481 aaaaaaagca gtggaacgat gagttacagg accaaatact atattcaata caccataata
     9541 cagcagcagt actactgtca tacaaagaag gctgaacatc aacttccgtc caaaatgccg
     9601 ttctttccaa aaagcattta gctcttttgt ttcaattcga acaaaatacc aaagcgtcac
     9661 acctaatcct aaaagggtag acaggcttgc acctgctgtt cctaataaac cggtcagaaa
     9721 agaagtagtc aatcctttta ccaatagtcc ccatcctgcg ccttttaaag aagggcgaaa
     9781 cgagttcttg ctttgagcaa tactttgata agcttggatg actgcagtga aagcaatgga
     9841 aaaaacaaat aatacaagcg ctgcatttcc cgcataatct ttaaacaacg cataattgat
     9901 ataagggagc agcaaggcta atcccaaaga cgcagctaat gctaaagcgg tcgtcaatct
     9961 aagatagatt ttggctgtct gtaaaaactg acgatagatc cggttggtca aatgacgagt
    10021 caacgcaggc aggaatgtcg cacttaatgc agtggcaatc actaaaccta attgtactaa
    10081 aggctgacct cgatcataga ccccttttgc tatcttcgca ccgtattctg acagtccgta
    10141 aacttccaaa gcatttttta caaagaaaga gtcgatcagc tggaaaaaga taagcaatcc
    10201 actataaatc gaaaccagtc cgccttctac cagaaaacgc cggatcaagg gctttgacgg
    10261 ctgtttgaac aatggaaacc gtcggaagct taacgctcct ccatgaatct tttgttcata
    10321 gtagtgaagg atcccatatg cgcataaacc gccaaaaaca gctccactca ttgctgccgt
    10381 ccctgtttgg tacaccgtcc atccaagctt tctaaaagca aacgcactac agacaatcac
    10441 aaaaacacga acgaactgct caactacttg agaaacagca gttggttcca tcaagaatct
    10501 tccttggaaa ttccctcgat aaaaagatag accaggcatc agcaaaaagg taaaggaaac
    10561 tacttggatc agcggtttca attgtatgtc tcccatcatc caagcaatcc agctgctaaa
    10621 tgcaaagaca aacaaccaca aaaagacacc tgtccaaaat actaagggat aaagattttg
    10681 caaagcttgc tttcgctcga aaggattctt tctttctgca acgtactttg atatgaactg
    10741 cggaagaccg gataaagcta aagtcattgc tatcccataa attggataaa cttgctgata
    10801 aacataaaaa ccttcgtcgc cgactaagtt ttggagggga attcggtaaa gggcacttaa
    10861 tacttttgct atgaaagaag caatcgtcaa tacaaatgct ccttgcatga cgcgccgcat
    10921 ttctttatga tccaaatcat tgctccttcc tacgctgaca gatatttttg ctcacgcaac
    10981 gctttgacaa acttttggat ttcctgaagc catactgctt cttccatttg gttggggatc
    11041 gtcaagcgta tcaccatctg ttctttttca acgcctagac tggcttttag ttttgtcgct
    11101 gtcagtgctt cgaataactg ttccacacta tagcgttttg ttccaacttt acttagagta
    11161 aagactatat gcgcctcctg tttccttatc gtttctagga gcgctcgatc gccatccatt
    11221 ttgatctgcc caattgtcaa gagatgtgct acttcttctg gatattctcc aaaacggtcg
    11281 agcagatcat cttctagttc atctagcatt tcttgcgaat caagttcacg aattcgttta
    11341 tagatctcga ttttctgccg ttgatctgcc acgtatgttt caggtagata cgcatcgaca
    11401 ccgaggtcga tctcaacagt ggtcttctcc acttgactat tctttccttg tttacgagaa
    11461 acagcttcgc ttagcatttg agaatacata tcgaaaccga ctgcatcaat aaatccatgc
    11521 tgctgagcac ctaagagatt acctgcaccg cgaatggaaa gatcccgcat cgcaatcttg
    11581 aatccagatc ccagttctgt aaaatctttg attgcctgaa gacgtttttc actgacttcg
    11641 ttaaggatct tctgctgctc atacataaag taagcatatg cgacacggct gcttcttcct
    11701 acccgccctc gcaattggta taacgtggat aatcccatat aatcagcgtt ctcaacgaac
    11761 aacgtatttg cgttcgggat atctactcct gtttcaatga tggttgtagt gacaagaaca
    11821 tcatattgac cttcaataaa ttcaaaaagc gtattttcta attgcgcctc tgtcatttgc
    11881 ccatgagcat aaccgatccg tgcttccgga acgagttctt ggatttcttc tactttttgt
    11941 tcgatcgttt ctacccgatt atataaatag aatacttgtc cacctcgtcc catttctcga
    12001 tggattgctt cgcgaatagc cccaggattt ttttccataa catacgtttg gatcggataa
    12061 cgattcgcag gcggtgtttc gattactgaa agatcccgca cgcctaacat cgacatatgc
    12121 aaagttctcg gaatcggtgt tgcagtcaga gtcaaaacat ccacttgtgc acgtaactgt
    12181 ttcaatcgtt ccttatgttt tacaccaaaa cgttgttctt cgtcaatgat cagtagaccc
    12241 aaatcgctaa attcaatatc ttttgacaaa attcggtggg ttcctacaac aatatcaact
    12301 tgacctgtac gcagttgttc gatggtttct ttttgttgct ttcttgttct gaaacgactt
    12361 aatagcccga tatttacggg gaaaccttca aatctttcca gcatcgtttc atagtgctgc
    12421 tgtgccaaga tcgttgttgg aactaaaaag gctacttgtt tgctttcctt tatcgcttta
    12481 aaggccgctc gaagcgctac ttctgttttc ccataaccaa catcacctac gagtagtcga
    12541 tccattggtt tttctttttc catatcccgt ttgatctccg ctgcacttct caactggtca
    12601 tcagtttcag agtaggggaa ggcattttcg aattcttttt ggtaggcgtc atcaggtcca
    12661 aaagcatatc ctttttctga ctctcttgcg gcatataatt tgattagatc gtcagcgata
    12721 tcctcgattt ttgaagagac ttttcgcttg gttttcgtcc attcacttcc gcctagctta
    12781 ttgatgcgag gcgctttggc ttcagaggcg acatattttt ggatcagatt cagttgagta
    12841 acaggaataa acagtttgtc gtcgttttga tacaaaatag tcatatagtc ttggtgaacg
    12901 ccatctactt ctagcgtttc catcccaatg tatttcccga taccatgatt ggcatggaca
    12961 acgtaatctc ctgcttttaa ctcattatag cttttcaatc gctctgcgtt tgttactgtt
    13021 tgacgtctcg tttgtttttt cgtcgttttt tggaaaattt ctttttctgt gatagctact
    13081 attttttctt gaggaagttc aaaaccagcc tgcaaagctc cttcaactaa ttgtacctgt
    13141 cctctgatca atgcattttt ttctgtttct actactaata tgtcttcatc tcgaagcatt
    13201 tgttctgctt tgcgtattct gtcctttgtc ggaataaaga tcacaacagt ctgtttttgc
    13261 ttgcgccaac ggtccatttc tgttttcagc aatggcattt gtccgaagaa ctgttgcatc
    13321 gtccgatatt gaaaattatg gattgcttgg aaacgaagat ttcccatccc tttttggaag
    13381 agtgagaaaa aggttgtggt gaacttttcc ttctgcacga ttttttgtat atctgctgaa
    13441 aatgtctgct cagaaaacac ccgcaattcg ctgatttttt gagtctgcca ttctgccgct
    13501 tccctttcga tttcccgatt tgtttccatg attcgctgat aatcatcaac gactagtaaa
    13561 ctatttgaag ggaaataatc caagatcgtc acagattctt ggtaaagaag atctgagtag
    13621 aattttgctt cttctgtcgg gacacctgct tgccagcttg aagtcagctg accgaagtaa
    13681 tcttgcaaaa actcgcggtc tgcttcttct gtggtcgcag caagccgttt ttctagcatt
    13741 ttttgcattt gctctattcc atgttctaga tcctctggtc cgtagacttg ttcactcgtt
    13801 ggggaaaccc agatttcttc cgttttttcg attgagcgtt gtgtttctgc ttcaaaatac
    13861 cgcatagaat ctatctctac atcaaatagt tctacacgaa caggataggt tgtcgttaac
    13921 gggtaaatat cgataatgct tcctcgaata ctgaattctc ccggttttgc gaccatctct
    13981 tctcgtacat agcccatcaa agtcaattgg cgaggaagat tttccacgtc tatctcatca
    14041 ccaatatgcc aattcaattg cgcctgtagc caagttttct tattcggcag tcgtttgtgc
    14101 aaagatgcga ctggcaacaa atagatacct gctttttttt cttgaagtgc gttaagcgtt
    14161 gcaactctct cgcctcgcgc ttctggagaa gaaaaggcca tttcagcaga aagcacttca
    14221 tccactggaa ataaatggat ctgatcttgc ggaatcacat gttgcagatc ttctgctaac
    14281 tgattcgcat aatacaaatt tggcatcaga ataatcatgt ttttatttgt tttcttaaac
    14341 gctcgagcaa atagaagtgt tttcgctgag cccgctaatc ctgtgattag ttgtctaggc
    14401 attttttctt ctaaattgct gacccattct tttacttgcg gatcttttcc gactaattga
    14461 ataatatcca ttccttttcc tccttcctca attgaatcga ttcatcgcat ccacgaacga
    14521 atgcccttct atgagatatt cgactgcttt aacagattga cacatgcttt cttctatcaa
    14581 aggctgattt tctttgctga aaggactaag cacatgctgg actactgttt ttttcccttc
    14641 tggcctgccg attcccacct tgattcgatc aaagacattc gtattcaaat gggaaataat
    14701 acttttgata ccgttatgcc cgccggcact tcccttttga cggagccgga tttttcccac
    14761 tgccagatcc aaatcatcgt agattacgac taattcctct ggataaatcc caaaataagt
    14821 cattagtggg ccgactgcac gaccagattc attcatgaat gtttgtggtt tgactaaaag
    14881 gatcttttct ccattatgaa aaaattcggc aacttctgct tcgaacggat tttttttgaa
    14941 agtagcttga tgctcgcgtg caattttatc cacgaccata aagccgatat tgtgttttgt
    15001 atattggtat ttggtacctg ggtttcctaa cccgacgatc attttcattt ttgttttcca
    15061 cctaattcta aaaagcagca caaaaggctg gactgctaat tagcggtcca gtccatcgag
    15121 cttttttctt ttttatactt gtcaatgcat agtataacac aaaacaaggc gtttcggcta
    15181 aaagagaatc gttaataaat gataaagaaa ttaaaaattt cttcaaagtt taatgaaata
    15241 attgatatat aaaaatagtg aaataacgca catgtaagct tttacatata atacaacctt
    15301 gttatctgtt atattttata gccaaatagc gtgacatggg gcttgagaaa tgttagcata
    15361 agcatgggaa aaaacaaatc aatggaagga ttgatactaa atgactgcag ctgcagaaaa
    15421 aaaagatcat caaaaagtta ttcttgttgg agacggcgcc gtaggttcta gctatgcctt
    15481 tgctctagtt actcaaaata tcgctcaaga agtaggtatt gtggatatca atactgctaa
    15541 aactgaagga gacgcaattg acttgtctca cgctttggca tttacttcac ctaaaaaaat
    15601 ctattctgct acttacgcag acgcacatga tgctgactta gttgttatta cagctggcgc
    15661 accacaaaaa ccaggtgaaa ctcgtttaga cttagtaaac aaaaacctaa aaatcaaccg
    15721 cgatgttgtc acacaaatcg ttgaatcagg atttaacggt atctttttag ttgccgctaa
    15781 cccagtagat attttgactt actcaacttg gaaattctca ggtttcccta aagaacgcgt
    15841 aatcggttca ggtacctctc ttgactcagc tcgtttccgt caagcacttg cggaattagt
    15901 tgacgttgat gcacgtaacg tccatgctta catcttaggt gaacacggag attcagaatt
    15961 cccagtttgg tcacatgcaa atgttgccgg cttgcaaatc tatgaatggg taaaaaacaa
    16021 cccagaaatc gatgaagaag ctatggttaa cctattcttt ggcgtacgtg acgctgctta
    16081 cacaattatc gagaaaaaag gcgctacttt ctatggtatc gcagtagcat tagcacgtat
    16141 cactcgtgcc atcttagatg atgaaaatgc tgttcttcct ttatcagtat tcatgaatgg
    16201 cgaatatgga ttgaacgaca tctacattgg tgcacctgct gttatcaacc gtcaaggtat
    16261 ccaaaaagta atcgaaattc ctttaagtga cagcgagcaa gaccgtatgg ctgcttctgc
    16321 taagcaatta aaagaaattc tagatgaagc ttttgctaaa ctagacgctg aataatcttt
    16381 gaacatgacg gaaaacatct attcggtgtt ttccgttttt ttattttatc acccttatgg
    16441 tttctaaatt ttttcataaa gtagagataa ccttcgtctt ttcctctaaa ttatgctatc
    16501 atgagaccgt tccaccctaa acttttactt aaggagttgt cgatatgaca agatcttcgc
    16561 atcgaaaaaa atcgaaacat gttgctttgc tttcagttct tggtgttctc ttagtgctga
    16621 tcagctttta tacctatcgc agtacatact acacaaagca ttttttgccg aatacagaaa
    16681 taaatggaat aaatgtcagc aatctaacag tcgaaaaagc aaatgaaaaa ttaaaagaag
    16741 cttattctga taaaaaactg gctattaaag aagatggaaa agtctggcag gaagtaccaa
    16801 aatccgagct cggctacaaa gatgatttca cttcggagct ttctccgatc ctaaatgaac
    16861 aaaacggatg gacttgggga atgacttatg tctcagcggc agaaaaacaa gaaatagatc
    16921 ctgtcagtca aaaccaccaa aaactagaca ctaccgttca gacattgaca actaagctga
    16981 ctgatttgaa caaagaccgg acacctacta ctgatgcaac gatcgaaaaa gcaggtgact
    17041 ctttcgttat caagcctgaa gtcaatggaa atacgatcga cgtcgatgct gcggtcaaac
    17101 aattaaaaag tgcggtaaac tctggtaaag atacaattga attgactgaa ttcaaagaaa
    17161 aaccaaaagt aacatctgaa gacagctcac tgaaagaaca actcgcatcc atgaatgcta
    17221 tcgcaaatgt aaaagctact tacagtataa acggtgagac tttccagatc ccatcaagtg
    17281 atattatgag ttggctgacc tacaatgatg gaaaagtcga tttagataca gaacaagtac
    17341 gtcaatatgt aacagattta ggcacaaagt ataacacaag tacgaatgac acaaaattta
    17401 aaagtacaaa aagaggagaa gttacggtcc cagttggcac ttacagttgg acgatccaaa
    17461 cagacagtga gacagaagca ttgaaaaaag cgattctggc aggccaagat tttactcgtt
    17521 cacccattgt gcaaggtggc acaaccgctg atcatccatt gatcgaagat acgtatatcg
    17581 aagttgatct ggagaaccag catatgtggt attacaaaga cggaaaagtg gcccttgaaa
    17641 cagatatcgt ttccggaaag ccaactacac ctacacctgc tggagtcttc tatgtttgga
    17701 ataaagaaga agatgcaact ttaaaaggaa caaatgacga tggaacacct tacgagagtc
    17761 cggtaaacta ctggatgccg attgactgga caggtgttgg tatccacgat tcagactggc
    17821 aacctgaata cggtggagac ttatggaaaa cccgcggttc acacggctgc atcaacaccc
    17881 caccaagtgt aatgaaggaa ttattcggga tggtcgaaaa aggaacgcct gtattggtct
    17941 tctaacaaaa ataatatata aaaagaaagc acccgtacga ttggtacagg tgctttcttt
    18001 ttattatcta acaatgtgct acttacattt cagcagcagt aaagacttct gaaacatcat
    18061 catcgtcttc tagcttatct actagacgtt ccaattgctc tttttgttcc tcatttaaat
    18121 caatcgtcgt ttgtggaatc atcgtcaatt ctgcttgcgc caaagtgaac tctttttcca
    18181 actcatctcg tacagcagca aaatcttctg gtgcagtata aatctcgaat acttcaggag
    18241 aagtttgcag atcttctgcc cccgcctcta atacatcctc aaacatcgtc tcttcatcaa
    18301 cagtcaaccc atcgcgttca atagcgatat atcctttgcg gtcaaacaag taactgactg
    18361 aacccgtttc tcccagtgat ccaccgtttc gagtaaaagc tactcggaca tttgtcgctg
    18421 tccgattacg gttatctgtc aatgcatgga ctaagattcc tactccaccc ggaccatatc
    18481 cttcatacgt gatttcatcg taattcgttt catctacggc actcgtagcc tttttaatcg
    18541 cccgttccac attgtcgtta ggcatatttg cagcttttgc tttgtccaca acaagccgca
    18601 aagctggatt catagatgga tcaggaccgc ctgctttagc tgccatataa atttcacgag
    18661 aaagtttctg aaagactttc cctcgttttg catcttgggc attttttctt ccttggatat
    18721 tatgccattt actatgtcct gacatgattt ttcctccctt gttcttttga gaatacactt
    18781 ctattctagt actatttcac aaaaaaacaa agccacagcc aatctataaa cgcttcttga
    18841 atgccgcaaa aagcacacat aatatcacag cagtcagagc accgatcgaa tccaacatga
    18901 catcttggaa cataggtgtt cgtccaccgg tcatcatctg atggaattcg tccaaacctg
    18961 cgtaccctgt tgctgaaagc caagcaagga tcgctgtcag ccaccaaatt tttagcttgg
    19021 ggaaaacacc taaaaacaag ctgcctccca aaataaaata ggtaccgaaa tgtgcgccct
    19081 tccttaaaaa gaactctaaa aatttggcgt agcccagatg gtcgatactc accatacttc
    19141 caccataatt aaaacgtata cccgctaatg tgtctttaaa tggctgatta ggcagccagc
    19201 tttctaatcg cgaaacttgt gattgctgtc cgtaagtttg agaagaactg taaaataaga
    19261 ttataacgac tatcagtgcg attactaaat aaatatttcc gtttttcatt tgtttttttg
    19321 aagaattcat aagtcacctc tgtattttat cttagacttt ttttgaagaa acaacaatca
    19381 ttttcataga gacttcttcc ataaaaaagt gctatactta attttgtatt acaatatgaa
    19441 gggagttttt tcatgagtta ttcattaaat tggaatctag attctatttt ttccggaggc
    19501 agtcattctg atgctttgaa ccaacggatg aagcaattgg aagaccaaat gaacgagtat
    19561 tatcaccgcg taacaaaatg gtcacctagt tctgacaatg cagagcagtt aaacgctatt
    19621 cttcaattgc aagaaacgat tacgaatggt tttacccaat gcagcagcta tattactgca
    19681 ttgctttctg ccaatgtcaa tgattcagat gcaaaggtct tatcaggcaa gctttacgca
    19741 atgctgcctc gtctgcaatc agccgaaact gtattgtcaa agaaatttgc agaaatatct
    19801 gacaatgatt ggaatcagat gctttctcaa tcttcgtttg aaacaattgc ttttcgttta
    19861 aacgagattc gtcgtgatgg ctcacaatta ttatcagaag ctgaagaaaa cattataaat
    19921 acgctttctt tagatggtct aaatgcttgg agcagtcatt atgatacgat tgtagccagc
    19981 atttcgattc ccttcgaaca agatggacaa gtagtcgaat tatctgctgg acaagccttc
    20041 aataagatga tgggcgatcc agatccaaaa gtcagagaaa cacttttcgc agcatgggaa
    20101 gaagcctgga aagaaaaggc acccctcttc acggatactt tgaatcattt agatggtttt
    20161 agactttctg actatgaact tcatggtgtc acagattttc tacaaaaacc attagagtac
    20221 aatcggttga agaaagaaac attgtctgtc atgtgggata caatccagaa aaacaaacaa
    20281 ccccttgtta attacttgac tagaaaagct aatcttttcg gtaaagaaaa aatggaatgg
    20341 caagatcaag atgcacctat tattttaggc gatttgaaag aaaaaacctt tagttttgac
    20401 gaagcagcag catttattat cgaaaacttt aacaaattca gtccaaaaat ggctaaattt
    20461 gcccaatctg cttttgaaaa gagctggatc gaagcagaag atcgtcctgg gaaacgtcct
    20521 ggtggttatt gtactgagtt accagaaaca aaagaatcaa ggatctttat gacctacagt
    20581 aattctgtta atgaggttgc gacacttgct catgaactag gacatgcttt ccatagcagc
    20641 acgatgtggg atttaccttc tttaaatcgc gaatatgcca tgaatgtcgc agaaacagca
    20701 agtacattcg cagaactgat cgtagccgat gctacattaa aagtcgcaaa aacaaaagaa
    20761 gaaaaaatca atttgctaga tacaaaactg caaaatgctt tagctatgtt catgaatatc
    20821 cattcccgat tcatttttga aaatcgattc tatgaagcac ggcaagaagg attagtctca
    20881 gaagataaga tcacagagat gatggttgag gcgcaaaaag aaggctatca aggagcttta
    20941 gctacttacc atccatattt ctgggctgca aaattgcatt tctttattga tgacgtaccg
    21001 ttttataact tcccatacac atttggctac ctctttagtt tgggcatcta tgcttacgcc
    21061 aaccaaaagg gagcaaattt tgaagaagaa tatatccatt tactacgcga tactgcctca
    21121 atgacgacgg aagatcttgc gaagaaacat ttgaatgtcg atcttaccca accagatttt
    21181 tggcaagcag gtatcgacat ggtgactgaa gatatccaaa cgttcatgga attgacagaa
    21241 gaatttatct gatttctatt ttatcacaat aaaaaaagag ttagtccaga atgggctaac
    21301 tctttttgat taatcattct tacatgctga cagcaaagtc aggggcaaac cattgaactg
    21361 aaccaacttt tacgctttcg cctggttgag gagcatggat atattgtcct ccgcctaaag
    21421 caatcgctac gtggtaagtt ccgccttgtg acccccagaa taacaagtct ccagcttttg
    21481 cttgagaaac tgaaattttt gttccagctg attcttgagg aactgtccaa ccaccgatgt
    21541 cacgaccagt tacttgcatg taaacatagc gtgtgaaccc tgagcagtca aagccacttg
    21601 gatctttacc gccccaaaca tatggagtac caatatattt gtaagcttct gctacgattg
    21661 ctgcaccatt cacacttcca gaaggagctg gagcaggtgt tacaggtttt ggttgttcag
    21721 gtgttggtgt tggcgttgga gccggagtac tacttcctgt gccgtttcct gtatcaacac
    21781 tttgatctgt tgatggtgtt gtaggcgcag gtgttgttga ttcttctgtt gttgaagatt
    21841 caggcactgt tgttgattct tctgttgctg aagattcagg cactgttgtt gattcttctg
    21901 ttgctgaaga ttcaggcgct gttgttgatt cttctgttgc tgaagattca ggcgctgttg
    21961 attcttctgt tgaagattca ggtgttgttg attcttctgt tgctgaagat tgtgtagctg
    22021 atgatgattc ttcagtcatt gaagattgtg tagctgatga ttcttctgtt gctgagcttt
    22081 gtgcagtaga tgaagcttga gtagcttgtg cttcagcctc tgcttgttcg cgtgcttctt
    22141 tttcagcttt ttcttgtgca gcttgttgac gagcttgttc tgctagacgt tgttgttcac
    22201 gaatacgagc ttgctctgct tcagcttctg ctttttgacg gttcaagtca gctttcttgt
    22261 cttcagcagt tgcttgttca gcagctaatg aagttttcag tacgttcaaa tcagcttgtt
    22321 tagataaaag atcgcctttt tgagattcca aagcagcttg gttttcagct aattctttta
    22381 gtttagcatc gttttcagct tttttgtctt ctacagcttt tttatcttgt ttttgttgtt
    22441 ccatcaagtc gttgtttgct tttaccatcg ttgtcattgc ttgaacgcgt ccaatagcat
    22501 ctgctaatga atcagcattc aatacagcat cgatgtagtt tgagcttgtg ttgcttactt
    22561 gtgcttcgcg tgcttgtttt tggattgtat cttcacgttt ttcaatacgt tcttgtaaat
    22621 ctgcaatgtc ttttactaat tgtgcagatt cttgacgcaa tgtatcttgt tttgctaata
    22681 ggtcttgtgc ttgtgtgttg atttcagaaa cttggctttc taaggcatcg atttgtgatt
    22741 gtgcatctgc ttgttgattt ttcaagtctg caatcttttg atcttgctgt tgtatctgag
    22801 aatcaaaatc gtccgccgcg gcagcgatcg gtgatgctac ggcagtcaag gtcattgaac
    22861 ataccattac tgctgatatt aaactctttt tcactcttca ttcctccgac tggcttaatt
    22921 taataaatat ctaaatatat ttaagtcttt cttaacgata taaaaattgt accatagcag
    22981 tgtgtcaacg atataatagt tatgttacaa aaatgtttca aaacaacttt taaaaatgac
    23041 aaaactgttc gtttcagaaa atgatcatga aaaagccgcg ttaagtctag catttcttaa
    23101 gataacaacg attctttttc gctttcgacc taaaaataaa aaaagaaatt attcatcact
    23161 gaataatttc ttaaagggaa aaatgaataa tacaaatata atcatgttca acagcagtgt
    23221 aggtcctaaa aaccttgtaa taaaatacgt gttatttact actgctaatt taaaaatcaa
    23281 ctggaccacc atcgtaaaca attcataacc cgtgacaaag atgatcatac tgaaaaattc
    23341 agtaaagatg ttgacatgga taacgtcttt cattacatac atcaaccata caattaaagg
    23401 gagagctact gcatagatac cgataacgcc aatatagtaa gcgtcataga tagccccgag
    23461 aacaatcgtg gtaatcagta aataacgttt ttcaaacgcc atactgcaac ataataatgc
    23521 caatatcaga aagtgggcat ttgacatata ggtgcctttc gaccactccc ctagcattct
    23581 tgttaaatga ccatctatca acatcaaaaa gaacaaaacg attggcagat agtacttcat
    23641 tgtttctttc ttcaacatgg actaatcctc cgctagccgt tggacaatcg tcacaactga
    23701 cacgtcatac atttctgcat aaggtttcac ataaacttcc cgatccagcc cataactatc
    23761 tggtttcgtt ttaataaccg taccgatcgg caagttagct ggagaattcc ctccaagacc
    23821 ggatgtctgg acaacatccc cttctttgat atccgtatca ccagttaatt gggtcaccac
    23881 taaggcatgg agcttttcat catagttttt aagcaaacca aacgcttcgc cattagccga
    23941 agatacccgt actggaaaat gattggagct ttcattagag gatgacagta attcgatttt
    24001 agacgaagcc gtattgacct cgatcactcg gccaatcaat cctttttgcg acatgactgc
    24061 catatttgct tcgataccgt ctttagaacc cttgtctacg atcagcatgt cctgccaact
    24121 gtcaggcgaa cgagtaatga catttgccgt tactttttcg taactagtca gcgtctgatt
    24181 caactcaagt tcttttttca acgcttcgat ttctctctcg taattcttgt tttgctgtga
    24241 aacgtcatca tagctatcga ttttttcttt tagccgctca ttttcggcat aagttgaaaa
    24301 aagattatgg acagaatcta ctccatttcg tacccaccgt actggggcag agattgtttt
    24361 atcaaccatt gcaacactgt cattaacgat ggattggaag aaattgggct ttccttcatt
    24421 cgctcgctgc gcagcggtca cacttagtat cgtcaccacg atgataacaa taaccaaggt
    24481 aataataata tttttattag gattgaactt cttcacaaag acacctcgaa ttaattagac
    24541 tcttgtgttc aattttatca ctaaatgccg tgaaacaaaa ggtaagttgc tcctcttaag
    24601 ctttttttta gcaaataata gattctgtca tttttagcct gaaagacaaa tttttttatt
    24661 tgtttatgga tcaatctttg ctttgaataa ctgcaatcat accatctgaa aaagaaaatt
    24721 ctgcggtatc tgaaagaaat tcgttacttg cataagatgt cggatattct acttctttat
    24781 tttccaatac atacttgctg ccttttaatg taagattcga gacgggagtc aagcagcaat
    24841 aagctaaata cttcatatct ggtatctgtc ttactatatg ctctccaggc aaataataag
    24901 acaaataatt ttgacaatct ctgataataa gctgatgggc aaagggtttg aagcgaggtt
    24961 ccatacctaa ccagagattg gctaaaagat gatccaatct tccgcccgta gctccaatca
    25021 aagtgacttc cgcttgcggg taacgttcaa atgtttttaa gatacctaac tgtgtatctg
    25081 tatcgtcttt ttctgcaggg gaacgggtat actctttggc ttttgcaaat acttccttct
    25141 gctcttcgtt cgacaatgaa tcaaaatcgc ccactgccag atctactggg tagcctgctt
    25201 gaagcaggta cagcgcacca cggtctatcc cgacataggc atcgtatttg gacagcagag
    25261 actgcggcca gttttgcgga ttacctccag cagctaataa aattttcatt tagtctaatg
    25321 cctcttttaa tcgttcgatt tgtccgactg gattttcggc tccatagata tatgatcctg
    25381 caacgaagac atccgcccct gcatctttac agatttttgc tgtttctgga acgattccgc
    25441 catctacttc gattgcataa gtccaattgt tctctctacg tagatcagcg agttccgtaa
    25501 ttttttctac tgtttcttca ataaagcttt gtccgccgaa cccaggattg acagtcatga
    25561 ctagtacttg atcagccaaa ggcaaaacat gtttgatcat ggagactggt gttcctggat
    25621 tgataactac tccagcttta actcctaagc tttttgtcat ttgaagtgcc cgatgaatat
    25681 gaggagttgc ttcgacgtgg acagtaatga tatctgctcc agcttttgca aatgctggaa
    25741 tgtagttttc tggatcaacg atcatcagat ggacgtctaa cggtaattta gtaacaggac
    25801 ggattgcctg aacaatattt ggccctaatg tgatatttgg gacaaattgg ccatccatta
    25861 catctacgtg gatgtaatct gcgcctcctt tttccactaa ctcaatgtct ctttgtaaat
    25921 tggcaaaatc tgcactcaaa atggatggtg ctatcttcat tcttgtttcc tcctattttc
    25981 gtttaccttc tttttttgca tacattggtt ttcttttttc gatttcttgt aagaattgca
    26041 aataattgtc gtaacgtgtc tgtgcgatac cacctgtttc tactctatgt tttacttcgc
    26101 agccaggttc tttagcatgc atgcactctc taaatttgca atggacagaa gcttctacaa
    26161 attcaggaaa ttgtctcggc agttctcctg cttcaattgt caaaaagtca atcgagctaa
    26221 atcctggtgt atctgctact aacccgccat agataggaat cagttccaca tgacgtgttg
    26281 tatgccgtcc tcttccaagt gcatcagaaa tctctcctgt tgctaaattc agttccggtg
    26341 atatttgatt cagtagcgtc gattttcctg ctccagactg acccataaag accgatagcc
    26401 gttctgggaa gtaacggatc agttctgtcg tatcacccgt atgattgggt acaatcaccg
    26461 gatagcctat tttagtatag gtttgcttga tctcttcgat tttttctggg tcgtctgaca
    26521 agtcggtctt tgtcaaaaaa ataatcggtt cgatcgactc gtattccaag gtaactagaa
    26581 agcggtcaag tagattataa gaaaatgcag gctctacgat acttgtgaca atgattccaa
    26641 gatcaacatt tgtgactggt gggcgaacta attgattttt tctcggcaag atttccagaa
    26701 gatagccgtc tgtttgattt tcactctcaa agatcacctg atctccgaca agcggcgtga
    26761 ttttccgatt ccggaaattt cctcttcctc ttgtttgata ggtctctccg tttgcgtata
    26821 catagtaaaa accgctgatt gcttttctga tttgtccttt gataagtgcc acctccgttt
    26881 ttgtttattt taccatacga agaggaaagt aggtgttatt ccatgcaaac ttctcaaagt
    26941 atatggcttg aaaattgatc aaaagagata gaaacacaaa aaagagaatg caacaaatca
    27001 atgtcgcatt cccttctgat atattttatt cagcacttat tttgaacttt ctgaggtgct
    27061 agctgatgaa cttgtggagc tggaagattc ttcttttgaa gagttgcttg tgctactgga
    27121 gctacttgag ctgctagaac tgtctgaaga gtctttttca gaactagaag acgctggatc
    27181 tggtccttta gagtaaatta cgttcacaac cattcctttg gtaattgtcg ttcctgcccc
    27241 tggtactgta cgaatcactt tatctttatc caccgtgttt gaatattcgg aagtctcatg
    27301 cccgatatat tctgcaccga cactagctaa ataactttgt gcttcgcttt ttgtatagcc
    27361 ggagatatca ttcaatttag gctctgttcc ttggcttacg acaaaattga tgtaagtttc
    27421 ttctgctaca acttcacccg aggctggatc ctgactgatg atcgaacctg atggtacagt
    27481 gtcgctatag tcataagtag cactaatatt attgatattg aatccttgtg taacaagcca
    27541 attacgaaca gtttcgtagt tttctcctac atgactactc aaagtcactg tttctggtcc
    27601 tttgctgact gtgaaagtaa tcgtgtctgt tttaggatcg acttctgtat cttctgatgg
    27661 agattgcgaa atgatcattc cgctttctac ctctgaactg aattcctctt ctttttgaat
    27721 caagtcagca gaaaacccta attctttcaa acttgcttcg gcatcttcat aacttttacc
    27781 actgtaatca tctaatttga tttttttcgt gccgctacta atatacagag tgacttcgcg
    27841 tttttgcttg actgtcgttc ccgctgccgg gtctgtcttc actacattac cttcttcgat
    27901 tttgtcatct gcaatttctt ctgtttttgt tgctacttct aatttcgctt ttgcgagtgt
    27961 ttctctagca gaagcttcag aaagatcaga aacatcagga acaacaactt ctcctcgccc
    28021 gccagaagca aagaatacag ctgttccgat tcctaaaaca gcaagaatag ctaataggat
    28081 cagccaccat tttttctgtc ctttcttttt aggatcaggc agaacttctt cctcctctgg
    28141 caaggtttcg ttttctggct tgtcttttgg cagaggcata gatttgaatg aatcaggcat
    28201 tggtgcatct tccgtaaggg gtgtgataac ttttgtttca ttatccatca catgaggctg
    28261 ccatttttct tcgtttgcac gagctggtga taatactgta gaaagatcat cagacatttc
    28321 ctcggctgac ttataacggt ctgcaggttc tttagccgtt gcccgtaaaa tcacgttttc
    28381 cagtgcttgt ggcacatttg gatcaagtgc ttttatagat ggaagatcat cttgaaaatg
    28441 ttttaaagca attgttacag cagattcacc atcaaacggt acgctacccg tcaacatctc
    28501 ataaaggatg atccctaaag cataaatatc tgactgcttc gttgccatac ttcctctggc
    28561 ctgctcaggg gacagataat gaacagatcc cagcatcgta ttcgtctgcg tgatcgatgt
    28621 ttcagataaa gcgatagcaa tcccgaaatc cgtgattttt acaacacctt cattatcgat
    28681 cagtacgttt tggggtttca aatctcggtg gatgatttga tggttatgag ctaaagaaat
    28741 agcagaaagg atctgctgca taatatttac tgctgtttca taaggaacag gataatgtgt
    28801 ttggatatat cgtttcaagt ccattccttt gacgtattcc atcaccagat actgcatgtt
    28861 gtcttcttca ccaacatcgt aaacactaac aatgtttgga tgaacgagtt ctgttgctgc
    28921 taaagcttct ctctgaaatc gtcgaatcgc agtctggtca ttttgaaagt caaatcgtaa
    28981 gactttaact gctacatcac gatccaagat taaatcgtgc gccaaaaata catttgccat
    29041 accaccgcta ccgatatttc cagtgatttg ataacggcca tttaattttt tgcccaactc
    29101 gatcatacac tgtcctcctt atattcgata agaagaacgg tgatattatc cgctccgcct
    29161 gcttcattgg cacgatcaat cagctcagtt actttatgag acaatgggcc ttcttgattg
    29221 atgatcgttc ctatttcttc ttcagacagc atgttcgtca agccatctga acataacagc
    29281 agtcgatctt ttagttgcca aagatgagta gaaacatcta cttcgactgt tccaggcatg
    29341 ccgacagaac gtgttaaaat atttttcctt ggatggttca ctgccatttc ttcgctgatt
    29401 tctcctgatt tgactagttc attgactaaa gaatgatcct ccgtcaattg catgatttta
    29461 tcttcacgaa tcaaataagc tctgctatcc ccaacattgg caatcgtgaa ccagtcatct
    29521 gacagagcgg ccgctacgat tgttgttccc ataccgttat attccggctg ttcttgtcca
    29581 cgctgataaa tcagcatatt ttcttcttga atcgattgaa tgaaccattg ggcgattttt
    29641 tcatcgtcaa cagcttcttg tttttcccaa gcttcgccta gattcgttac cgccatctga
    29701 ctggcaatgt ctccggcacg atgaccaccc atcccgtctg ctaatacagc aagcttgaga
    29761 cctgaacggc tggtaaatac actggcatag tcttgatttg tgttacgtcg tcttcctaca
    29821 tctgattgat attcaatctg cattttttca cctcattact ttttgcgcaa acaacaaatg
    29881 aaaaatccat ctgtgtaaaa ctgatgcgga tacaaagtca acattttctc ttcgattgat
    29941 gattgtagca taggattggt caaaacatca atcctctcaa aatcagggtg tctttccaag
    30001 aaagcagaaa caacgtcttg gttttcttct tttaagatcg tacaagtgct ataagtcaga
    30061 ataccggaag actttaaagt tggtgcgcag ctttctaaaa tttccaattg gatttttggt
    30121 aaattcgcta attcatccgg ctgtttttta taacgaatat ctggttttcg ccgaagcagc
    30181 ccaagacctg aacaaggcgc gtctactaaa atccgatcaa actgctctgc ttcgaactgt
    30241 ttactgactt ctctagcatc catcttttga gcgtagacag cagaagagac acctagacgt
    30301 gctgcattat cttcaatgag tttgactttg tgttcgtgta catctaaagc gaccacttct
    30361 ccaccgcatg acggatctaa aaaagaagca atatgcgtcg tcttacctcc gggtgctgca
    30421 caggcatcta agacatgatg ttcaggctcg atttgcatag acggcgctac aagcatcgaa
    30481 ctttcatctt ggatcgtcat gacgccatcg tggaacaatg agctagaagc taaatgccct
    30541 tttttcgcta ctataccgta tggactgact tggctttttt cagctgtgat ctcttcttct
    30601 tgcaaaacag tgatggcctg atctctggaa agtcggcgtg tatctactcg ggcactagca
    30661 tggctaggct gatatagcga taaaccgagc ttttcagttt cttcgtatcc gatttgatcc
    30721 accagtcgtt tcgtcatcca tagaggcagg ctgattttga cagaaagtct ttcgatagga
    30781 tcagcaatgc cttgcagatc tggtgctcct tgacgctgat aattcctcaa gacaccattt
    30841 acaaatttcc ctgtcccagg atttcctttt acttttgcga tttcgaccgc ctcgtttaaa
    30901 atagcatgtg cagggacttt atctaaaaat tctaattggt atacagaaag ataaagaagc
    30961 gtcttgaccc agtcatccac tttttttgct ttagcgatca atggtgcaac gtaaaattcc
    31021 aatagtcgct gatggctaat cgtaccatag atcaattctg taaataaacc cacatctttt
    31081 tgatttagct cgctttgttt gatcatctca ttcaacaaaa gattcgagta ggcgccaccc
    31141 ttgttcactc gctctaatgt cagtaatgcc atatatcgta ctgatttttt cattttttta
    31201 gggatttttt tcattatttc acctgctgac caatctgaac ttgctgaccg cttccgttaa
    31261 ggaaatcatg tacggcctgt tttcctttac cagctggctg taattcattg atggctaaga
    31321 cggtctgttt accgcaagca atccatagat tttttttgtc tttccggatg atcgtaccag
    31381 gtgctttgtc tgtagtttct gccaatactt caaccgataa taatttccag cgtatgccct
    31441 cataggtcgt gaatgcaatt ggccaaggac gcattccgcg tacttggttg tcaatttctt
    31501 ctgcagtttt atgccaatcg attgcttctt gttcccgtgt gatgtttggc gaaaaagttg
    31561 cttctgactc gtcttgaggt actggttttt gtccatccaa aatattgggc aatgtttcta
    31621 gcaatagctt tcttcctaaa aggctaagct tttcaaacat cgtccctaca tcatcttgtt
    31681 tagtgattgg tatgctttct tgcgcataga ttccgcctgc atccattttt ttgatcattt
    31741 ccatgatcgt tacaccagtt tctttctccc cattcatgat ggcataatga acaggcgcac
    31801 ctccccgata tttaggaagt aaagaagcat ggacattgat tgcccctaat tttggtactt
    31861 gcaataactt ttcaggcaag aattggccaa atgccgcagt gacgatcaca tcaggcacca
    31921 attcaatgat ttcttccatt tctggcgaac cggagatttt ctctggctgc agcacacgga
    31981 taccgtgctt cagagccgct tcttttaccg gtgtcggcgt aattactcgt ttccgcccaa
    32041 ctggacggtc gggctgtgtt accactgcct ggatctcgta acctgattct atcagccctt
    32101 caagaatcgg cacagaaaaa gcaggtgtgc ccataaatac tagcttagtc atcaagatgt
    32161 tcctccatat attcttcaag ttcttcttcc gggatctgct gtatcatttt ctcaatgaac
    32221 aagataccgt ctaagtgatc gatttcatgc tggaaggcac gggctaaata gccaaaagcc
    32281 gtcacttcta tctcttctcc gtctcgatca taataacgca cggtcacttc atctgctcgc
    32341 ttgaccgttc catagacatg cggaatactg agacaacctt ctacgtcaag gctttcacct
    32401 tttgcttcga tgatttctgg gttaatcagt tcaaatttct cgccttcatc tacttcgatc
    32461 acagcaattc tcttgttttg tcctacttga ggagcagcga tccctattcc gtcattcgca
    32521 atcattgtct catataaatt gtctaataaa gcgattgtct cgtccgtgat tacatcgatt
    32581 ggctgagcag ttctttttaa tttatcatta gggtggatca atataggata gcgcataatt
    32641 tggccctttc ttaaataaag tttaatggtt cgttgtcaat tgccacaaaa agaccttgtc
    32701 gctgttcttt ctggcttcca ttcaaaattt catccaaaac atgaggaaga ttcggttcat
    32761 gtttatattt gatgattagt tgataataat atcggttttt cactctcaaa atcatgctgg
    32821 gcgttggccc taatagcaag ctttgagggc ttaacacttc ttttagctga ttggcaatct
    32881 gaaagatttt ctttgccgct acttgttctt caggatggct ggcggttatt ttcacagtaa
    32941 agtaataagg aggatagcct ccacgatgcc gtacatgcat ttcctgctga taaaactgct
    33001 cataattttg ttttttagct aattctatgg cgtaatgttg aggattgaat gtttggatca
    33061 cgacttcgcc tgctttttcc gctcgccctg ctcgtcctga aacttgggta agcaattgga
    33121 atgtatgctc actagaacga aaatccggta aattcaggga ggtatcagca ttcaacaccc
    33181 caactaatgt cacttctgga aagtccaaac ctttagcaat catttgtgtc cctaataata
    33241 tatctgcttc ttttgcaccg aatttttgta aaatctgttc gtgtgcccct tttcttcgag
    33301 tggtatctac atccatacgc aaaatcctag cttgcggata aagttctcgg agttcctctt
    33361 ctactttttg agtacctgtt ccatagtagc ggattttatt gccgccacaa ttaggacagc
    33421 gattgggaat aggttcttca tgtccgcaat aatggcagcg cattgaacgt gtatccatat
    33481 gcagtgtaag agagatatcg caattaggac atggaagaac aaatccacag tccctacaca
    33541 tcacaaaaga cgaatatcct cgacgattga gcagtaggac agtttgttct tttttgtcta
    33601 atcgattttg tattttttcc tgcaaattag cagaaaaagt agaggtccga tgattctgaa
    33661 actcttcacg catatctacg atctcaatag ctggaagctg tgcagctgct ttggctcggt
    33721 ggtttaactg caataactga taaacgcctt tttgagctcg tgctcttgat tctaaagagg
    33781 gcgtcgcgct tcctagaact attgggcaat gatgatattt actgcgccaa attgctagat
    33841 ctctcgcatg ataacgcggt gtctcatctt gtttgtaact tgattcatgt tcttcatcga
    33901 taatgatcag cccgatattt ttcagcgggg caaaaatagc agatcgtgcg cctactacta
    33961 cttgcgcttc ttcccgctca atcttgcgcc attcatcata tttttcacct tgggaaagtc
    34021 cgctatgtaa gaccgcaact gattctccga agcgtccttt gaatcgctcg accatttgcg
    34081 gagtcaatgc gatttcaggc actaacataa tagccgtttt attttctgac aaaacatcag
    34141 cgattgcctg aagatatact tctgttttcc cgctgccagt gattccctcc agcaaaaaga
    34201 cctgatcttt ttgctgctga cctgctgtga tgatttgttc gacagcattt ttttgttctg
    34261 cattcagttc caatgccgtc gtttggtcaa atactcggtc tttataaggg tcgcgataac
    34321 gttctgattc tataaactcc agccagccct tttcttttcc ttgattcaag acagcagtac
    34381 tgattccttt atttttgaaa taagcaattg gttcttcttg gctcagttca gataaacaca
    34441 aaagaagttc tttttgtttc ttggcatttt gccgtaattt tgcccattct tcttcgattt
    34501 gcttttcagt taacaaagaa cggaccaaac gaatcgtttt tactttattt cgtgtatgga
    34561 cttcatattt gatatctact aattgctctt ggcgccagcg aatcaattga gaaaggctgc
    34621 cttcttcttc tgcttccttc caggaaatca cttctcgctg atcaaacaat acctgaactt
    34681 ctggtgtgtc tgccaaagga tagatcaatt tatcatattc tgctttcatc acactcggaa
    34741 gcatcgtttg tagacaagtg attttgaatg cgtaagtaat ttttttcata taatcagcta
    34801 atgaaagtaa ttcttcattg accaccggag caaggtctag caaacgaata actgccttca
    34861 aagatggatt agctgattct tctgattttt ggatagccat aacaaatcct tggacatgac
    34921 gattgccatt accaaaaggg acttctactc gcatcccgac ttgcaaagct gtttccattt
    34981 cagagggaac caaataagtg aatggctgat cggtctgcat agtaggtacg tccacaatga
    35041 cttcagctgt taatgtcatg gttcgtttcc tccttttttt gtcttttctt atcaagcgac
    35101 aaaaagttcc ttgcctattt tactagagat cgatggattt gtcttatctt ctgccaaatc
    35161 ctaacaaaaa attaacagca gataagaaaa aaaagaactg tgtcaaaaag ttcagacaca
    35221 gccttttcta ccagacaata agtggcgaat gtttgaaaag gaaacagctg ggtttgcttt
    35281 tctttttaga tgttcgctct atttttcttt attacatttt ttgctcttca cggatacgtg
    35341 attccaattc gtgttgttca cgctcttttt gtgcttgacg ttcctcttct tccatcttca
    35401 aacgttcacg ttttaattca ggatgtggat cattgactac atttcctgca tcgatttctt
    35461 ctaaagcttg accgacgctt tttactgaat caaaagaatc tagagtaggc tgtgcacttg
    35521 catccaattc atgcgcgcgt ttacttgcta aaatcactag agaatatttt gaatttacac
    35581 gatctaatag tgagtcaatt gaaggtttta acatcataag actacatctc ctttaacatt
    35641 ttaatatatt taccgattac gcgatctact cgaaaatgtt cactggcaat aatgtttttg
    35701 attcgttcaa ctgctagcgg cacttcatca tttacaacag catagtcgta aagagccatc
    35761 atttcgattt cttcttttgc aactttcatt cgttcttcga ttacttcatc tgcatctgtt
    35821 cctcggccta cgatccgtga ttttaattcc gctaaatctg gcggagtcaa aaagatgaat
    35881 acaccatcgg gcactttttc tttgacttgt tttgctcctt ggacttcaat ctccaagaaa
    35941 acatcttttc cttcatccaa tgtcttgttg atatatgaca aaggagttcc ataataattc
    36001 ccgacatatt ctgcgtattc cagcatctct ccagcttcga tcattgattc aaactcttct
    36061 ttgcttcgga agaaataatc aacgccttcg acctcccctt cacgcatttt acgggtagtc
    36121 attgagatcg aatactgaaa atcattatcc tcactctcaa aaattgcttt tcgtactgtg
    36181 ccttttccta caccggatgg accagacaac acgattaata atccacgctc tgacatgacg
    36241 acgtcctttc aactagtctt tttgtactta tcgtttattt tgcacttttt ttctgcaact
    36301 ttcaaggtta tctttaatta tagctagaaa ttggtaaaaa agctaccctg taccaagagg
    36361 tcgtaaaaaa gctgtcctgc atcaagtaat aagaacagcc aaacataaac agcttctcat
    36421 gtttttcgtt tggttggggt tattatcgca gatgcaagct tttgaacgcc gtgtataaga
    36481 ggtcgtgaag aagccgatta gtttcgagta tgaagaaaaa ttttgtgaaa atagtttctc
    36541 acatttttgc ataaatttgg cttaata
//
LOCUS       DANKEF010000024        34770 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000024 DANKEF010000000
VERSION     DANKEF010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34770
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.024"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(226..1113)
                     /locus_tag="PWZ48_001353"
     CDS             complement(226..1113)
                     /locus_tag="PWZ48_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2261021.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1110..1979)
                     /locus_tag="PWZ48_001354"
     CDS             complement(1110..1979)
                     /locus_tag="PWZ48_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2261022.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2116..3402)
                     /locus_tag="PWZ48_001355"
     CDS             complement(2116..3402)
                     /locus_tag="PWZ48_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2261023.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3675..4253
                     /locus_tag="PWZ48_001356"
     CDS             3675..4253
                     /locus_tag="PWZ48_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2261024.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4253..5182
                     /locus_tag="PWZ48_001357"
     CDS             4253..5182
                     /locus_tag="PWZ48_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2261025.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5179..6915
                     /locus_tag="PWZ48_001358"
     CDS             5179..6915
                     /locus_tag="PWZ48_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HDL2261026.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="PWZ48_001359"
     CDS             complement(6978..7814)
                     /gene="pheA"
                     /locus_tag="PWZ48_001359"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HDL2261027.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7811..8320)
                     /locus_tag="PWZ48_001360"
     CDS             complement(7811..8320)
                     /locus_tag="PWZ48_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HDL2261028.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="PWZ48_001361"
     CDS             complement(8313..9608)
                     /gene="aroA"
                     /locus_tag="PWZ48_001361"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDL2261029.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(9621..10712)
                     /locus_tag="PWZ48_001362"
     CDS             complement(9621..10712)
                     /locus_tag="PWZ48_001362"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HDL2261030.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="PWZ48_001363"
     CDS             complement(10729..11895)
                     /gene="aroC"
                     /locus_tag="PWZ48_001363"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDL2261031.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="PWZ48_001364"
     CDS             complement(11897..12961)
                     /gene="aroB"
                     /locus_tag="PWZ48_001364"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDL2261032.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="PWZ48_001365"
     CDS             complement(12994..14016)
                     /gene="aroF"
                     /locus_tag="PWZ48_001365"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HDL2261033.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14675..15649)
                     /locus_tag="PWZ48_001366"
     CDS             complement(14675..15649)
                     /locus_tag="PWZ48_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HDL2261034.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15630..17513)
                     /locus_tag="PWZ48_001367"
     CDS             complement(15630..17513)
                     /locus_tag="PWZ48_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HDL2261035.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17531..19756)
                     /locus_tag="PWZ48_001368"
     CDS             complement(17531..19756)
                     /locus_tag="PWZ48_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2261036.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19871..20905
                     /locus_tag="PWZ48_001369"
     CDS             19871..20905
                     /locus_tag="PWZ48_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2261037.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(20972..21985)
                     /locus_tag="PWZ48_001370"
     CDS             complement(20972..21985)
                     /locus_tag="PWZ48_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2261038.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(22063..22251)
                     /locus_tag="PWZ48_001371"
     CDS             complement(22063..22251)
                     /locus_tag="PWZ48_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDL2261039.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(22691..22819)
                     /locus_tag="PWZ48_001372"
     CDS             complement(22691..22819)
                     /locus_tag="PWZ48_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDL2261040.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="PWZ48_001373"
     CDS             complement(22822..24195)
                     /gene="rlmD"
                     /locus_tag="PWZ48_001373"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDL2261041.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(24306..25361)
                     /locus_tag="PWZ48_001374"
     CDS             complement(24306..25361)
                     /locus_tag="PWZ48_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2261042.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="PWZ48_001375"
     CDS             complement(25371..26801)
                     /gene="gatB"
                     /locus_tag="PWZ48_001375"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HDL2261043.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="PWZ48_001376"
     CDS             complement(26798..28270)
                     /gene="gatA"
                     /locus_tag="PWZ48_001376"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HDL2261044.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="PWZ48_001377"
     CDS             complement(28270..28575)
                     /gene="gatC"
                     /locus_tag="PWZ48_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HDL2261045.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="PWZ48_001378"
     CDS             complement(28681..30717)
                     /gene="ligA"
                     /locus_tag="PWZ48_001378"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDL2261046.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="PWZ48_001379"
     CDS             complement(30734..32968)
                     /gene="pcrA"
                     /locus_tag="PWZ48_001379"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HDL2261047.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            33123..34682
                     /locus_tag="PWZ48_001380"
     CDS             33123..34682
                     /locus_tag="PWZ48_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HDL2261048.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
BASE COUNT         9518 a         7494 c         6335 g        11423 t
ORIGIN      
        1 acaatcagga cagtcatcgg caatatagca gcaatcactt ctttaaaatt ttttagcatg
       61 tatcctcctc ctttactctt taagtttacc ttaaaatgaa agagttttcg agcacaaact
      121 taaaacgtta tcatttaatt tttctcaaca taaaataaca cacgtcccaa aagtatcttt
      181 ttacttggaa aaatgacttt gaaggaacgc gcttattgtg tttttttatt ttacagagcc
      241 gatcattcct tgaacgaatt gcttttgcaa aacaaaaaat acgatggcag ttggtaaagt
      301 agcgatgact aatccagcca tgatcattcc atagtctggt gtataggaag cacccatagc
      361 agataaaaca agcggaaccg tccgtttatc aggagattgc aaagctacta agggccataa
      421 ataattgttc cagctactca tgaatgtgat gattgcagcc gcggcatagg tatttttagc
      481 cgtcggcata tacactctaa aaaaaatact tagttctttt agaccgtcta atcgagcggc
      541 ttctactaga tcttttggaa aagatttaac attttgtcga aagaagaaga tcaaaaaagc
      601 agtgctaaca gatggcagga tgacaaccaa gaaactgttg attcccaaaa ccgttccgtt
      661 caattgtgaa aacattcgat ataaagggat catcaatgcc gcaaaaggaa ccatcatcga
      721 tagcagtaga atattgaaaa ttaggtcttt tcgtttactt tgataaatct caaatccata
      781 tcctgccatt gacgaaatca gcaatgcaaa cacagtggtc accaaagcga tcatcgctga
      841 attccaaagg gaacgagtaa aattcagctc attggaaaac aaattttgaa aattgatgat
      901 caagttactg ccaatcttta tttttcccga tgtgatatcc acgggtgtat tcgtcattcc
      961 caaaaccatc cagacaaaag ggaaaatgga aataaatgat aggatgctta atgctgtata
     1021 ctttaatact gacccaattg tcgaacgaag ttctaatgct tgttttttga cgttggggcg
     1081 ttcgattgtt tctttttcta ccatattttt catcgacttt ctcctcctac tttcgtttgg
     1141 attatcgaca atatcacaat aaaggaaaca ataacaaagg aaactgctgc cgcatatcca
     1201 aaattaggcg tgaatttata actcaaatta tagatgtatt gagatagggt ggtcgttgca
     1261 tttgctggtc ccccgccagt aatattttgt acttcatcaa aaagctgcaa tgtcccgatc
     1321 gtcgaagtaa tggaagtaaa caaaatgatt ggttttaaat ttgggatcgt cactttgata
     1381 aactgctgcc atttagaagc accgtcaata gatgctgctt cataaatctc cgaatcaata
     1441 ttttgcatgc ctgaaaggaa aaaaatcata ttgtatccag tccaacgcca agtgatcgaa
     1501 agaatgatca gaactctcgc ccaaattgga tgagtcaacc agccaatcgg tgtgtcaatc
     1561 ataccaatgc ccaacaacaa gttgtttaca aggccagatt gagaaaatag acttttcatg
     1621 atcagagaat aactgaccat cgaagtgata catggaagaa agatagcggt tcgaaacaac
     1681 cctttatatt tcaacttttt atcatttaaa aggttggaaa taaaaagtgc tagaaacaac
     1741 atgatcggta cttgtacaag taaatacagg aatgtattaa aaaaagcttt tttaaaagtt
     1801 gcatctgtca acatccgctg ataattacct aacccgttaa atgtcatatt gacaggggga
     1861 ccactttgaa aagacgtgat caaagccaaa accatcggaa taaaaaccat gattgatatc
     1921 aaaatgactg gcaagattaa aaaagcatgc ccgtttttga ataaatcttt ctgcttcatt
     1981 cttttccctc caattctatc attgattatc tacatgaaaa gacaagaaag acaatatctg
     2041 taaggcagac gtgatctttt ttgtcttttc gtttgtctga tgaaaaagcg aagaaattca
     2101 cttttttacc accgatcaat tagccaattg tgcttctact tgtttttgtg tatctgctag
     2161 taccttgtct gtctcttctc cattgatgat tcgatgaaga gcttcagcta cgactgcttc
     2221 aattgcatag gtctcatgtc cgtaattcac ttctggtatt tcagatgtcc atgttgaaaa
     2281 atcgtcaaag atcttttgac cactgtaaaa ttcagaagct tcttgataag cttcttgttc
     2341 attcgcgcta agcatcgtag atactagtcc gatttctttc gcaagagtgc ccatcagttc
     2401 ttcattcgaa gcaaacgttg cttctaggaa atctttcgct gaatcttctc cagctactcc
     2461 tttgatcaca taccagccag cgccgccaag gttagaagct tgcgcttttt gcagattttc
     2521 tggaagtgca ggtattggtg caatcttcca ttttccagat tggttttctg ctccttggat
     2581 cgttgaagaa taccaagcgc cagttgggct agaagctact gctcctgatt gtatcgcagt
     2641 tactccagca ttccagtcag aggtttgttc gactagatct tctttcaaaa gagtggcaaa
     2701 caattcaaga ccatacttta aagatgcatt gtcttggatc gtgactgtct ccccatcttc
     2761 agatgtatac cattctcctg cctgttgcat aatcatcctt actcgaccaa ggtcagaagg
     2821 attgacttca gcgattttct ttcccgtctt ttctttgaca tctcgcgcta cttgaatata
     2881 ctcgtcccac ttcagattct ccatatcctc ttctgtataa cctgcttctg ccatcagatc
     2941 cgtacgatag aagtttgctg ttacgcccga atcgaaagga acaccgtata ttttatctcc
     3001 tactttgttt accgcaaatt tatacggtgc aaaatgatct tcctttacaa tagcagacaa
     3061 attagcgaat gcatctggat aagaagtcaa atagccttgt atcctataat cttcaatcaa
     3121 gacgatattg ggcaatcctt ctgtattccc gctcgctaaa gcggtgttca gtttctgaac
     3181 gatatcatcc tgagacatcg taacaacttc tatctctgtt tcttcgttct cataaacttt
     3241 ttttgcttca ttcactgctt tgatattgaa tgtctcatcc cacgcccaga cagtgacttt
     3301 ttcttctttg tctgctgtac tactagcact tccgcatcct gttaagaaac ctgatgctgc
     3361 cgctaaacaa acgactccag caattaactt tctgatgttc atttttatcc gctccttttc
     3421 catccattag ttgtttacgt ttgctacatt catcatacca aaatttgaaa acggtttctt
     3481 tattattcag atacttcaaa aatttctcct aaaataataa aaaaatacaa atcaactatg
     3541 acaaaaggaa taatcttagc atctagacca aagatcgaaa tggtgaggtt tctttaaaat
     3601 aaaaatatcc ttgcattttc tcctagaaat aagtattttt aaattacaag actattctta
     3661 aggcgggtaa aaaaatgcca atacgcgtaa ttcttatcga tgatgaacag acgattttag
     3721 atggaatgaa acagttgatc aattggtctg agcacgggtt tcaattaata gggacttttt
     3781 ccagtccgct gaaagcgctt gatttttctt tggtacatgc aatcgatatc gtggtcacag
     3841 atgtaatgat gcctgaatta aatggaatcg aattatcgcg gctattaaaa cagcaacaac
     3901 ctgaaacaca gatccttatc ctctctagct atgatgaatt tgatatggtt aaagattctt
     3961 ttaaagaagg cgtcgctgat tatcttttaa agcctcgatt gagtcctgat ttttttctaa
     4021 attccttaac tgccgtttca caaaaaatca aaaaaaagcc caacaaaagc ccaatcacca
     4081 atcgttatga gcagatctgc gagaatcttt cacagcagat tgctggacaa actcgtccat
     4141 tgcttgaccc tcttcttttt gaacacacct tgtttttctg ttatataccg aaaaacggcc
     4201 attcagttca aaacaaggct ctaaacaaaa acagtcgatc acccattcat tgatggatag
     4261 cgacgattcg tttactattt atcctgtttc taccttttcg gaagaatccg gctatgtaat
     4321 caatactgat tccaaagata cacttaggaa gtttgttcaa aagtttcgtc aagaagtttt
     4381 tccaggtgat ttttttgttg tttctgattc ttttgcaatc caagatatca atgaaatatt
     4441 ccaagagatc agaaattgca ccaaaggaca acgtttctat cataagggaa agaaccttgc
     4501 ttatcagcat gaacttcttc cgttattaac caaccacgag cagcaagcaa gtagttattt
     4561 aacaaaaatc gttcataaag actacgcttt agctgtcaaa gaacttgaga atttcttcac
     4621 gacgatattg gaacagccta ttgatccagc ttatttgaaa catgaggcaa tcaatcgttt
     4681 ctatgcatta ctcaacgctt tggctgacga gcatgttggg aatgaagaac tttctttgct
     4741 caagatcgcc cttccttctt tattagccga tgctgagtat ttcgaagatt tttctattct
     4801 tgtcaaagaa acacttgaga aactcaccca cttttctgca aatgcaaaaa aagacaattt
     4861 cgatatcttg actttgatct acgagttcgt ccaggaaaat tatgagaaag aaatcaattt
     4921 acaactttta tccgaaaaat atcatttttc atatagctat ctctcagcca tctttactga
     4981 aaaatttggt atcaactttt caaaatatct aaaaaaagtc cgtatcagta aagccaagct
     5041 ctttttgacg gaaacacggc ttactttgac ggaaatctgt gagaaagtcg gctataccga
     5101 actgggttat ttctccagag tttttaaaga agagacagga atgacacctt ctcaataccg
     5161 taaaggaact tttgtaaaat gagaaaaaaa atcaaagagc atgaactctt tgccaaactt
     5221 ctgttcattt tgttcatcgg tctttttctg caagccatcg tcatttcttt gtttatttat
     5281 catcgatcaa gggatgccta tatccagctg ttcaatcaat cgaatgatgt ggtactaaaa
     5341 aagattcaat ctgaatttga aagtttgaat gatacaatcg agaatacatt agccgctttt
     5401 gattctaatc cagctgtcaa aagctatttt tccaatgatc cagcccaaca tatggaacag
     5461 cttcagcaac taagaacaat ccaaaaaatg aatgactctc tttcaaaaat ccatccaatg
     5521 atcgattacg atgtattgat ctttggagaa aatggaagaa cttttgtcgg aaacgacatg
     5581 ctcactgccg tatctgccga ttcttttttt caatctgcga ttgctcagag agtgaatgaa
     5641 cgtgctgctg atacacaaat gctttttgca caccacggat tgactcttcg cgataagaaa
     5701 gcacccagcg tcttctttgt caaaaaactg aaaaatacat tgaatcacgt ttacggatat
     5761 gctgttttat cgatctcatc caagcaacta gcgaatcttt ttcaatctgt agtaaatcct
     5821 gagatatcga agatctcctt catgaacgaa gaacagacga tcatcgcatc aaatgaagaa
     5881 aatacaattg ggagccgatc aatgattttc aaaaaactaa cagtaggtga aacaacgatt
     5941 tcaaatggac agcagttgac ccgtcttcct ctgtaccgcc aggattgtac attactcagt
     6001 caagtcgatc ttcattcttt atctaaccaa atgggcgtga tttttccgat tattttgtat
     6061 aatatcgtga cacttgcttt tgctggctca ttggtattta tgtatttgga tcgacacaca
     6121 aaatcgatct accgcttgat tgattcgcta aaacggctag aggaagatgg cttaaaagct
     6181 actgttcctg tacaaggaag ccatgaagtc caattgctag ggaatacgat caatcaattg
     6241 ctaagtaatg ctcagcatca ttatgaacag acgatccaag acgaaaagaa gaaacgttcg
     6301 cttgaaatcc aaactatgca agcacagata cagccccatt ttatttataa tacgctgact
     6361 agcttgaaat ttttgatttg gcagaaaaaa acagatgaag cagttcaagg aatcgacagt
     6421 ttcattactt tactaagaag tacgattggg aaaaaagaag aagtgattcc agtcaaggaa
     6481 gaaatcaaaa gcgtacaaag ttacatcgat cttttgagta tgcgatatgg cgaaagaatc
     6541 tcggctaaaa taatgattcc tgatgaactg cagtcattat ctatgccgaa tatgatcctt
     6601 caaccaatta tcgaaaacgc ctatcttcat gcttttcaca ataaaacgca aggattcatc
     6661 acggtgtacg gcaaaacaaa aaatcagctt cttttattcg aagtgattga taacggcgat
     6721 ggcttcgata ctgaaaatac caaacaaaaa aatgatcatt tttcaggaat cggcatcagc
     6781 catgtagatg agcggatacg cctaatgtat ggcgatgcat atggcttaac gatccaatca
     6841 gtgattggga caggaacgac tgtaacgatt tgtctaccgc ttcttcctaa aaatcaatca
     6901 gaatatcaaa aataaagaat agccaagcgc tgattttttt attttaagtt aaataaatca
     6961 atcagtattc ttgactttca tctacgcgtc tcttctactg tcagcactgg ataacagccc
     7021 aatatttgac tttctcctcc gagcatcttg atttcttgga gcgcattctc tactaatgtc
     7081 attggacggt ccaacaacaa atcgatcacg aaaaaatatt ctcccaagct agttttcaat
     7141 ggtcgggatt caatcttact taagttgatt tcccgccagc caaaagctgc cagcatttta
     7201 tgaagcatcc ctggccgatt agcaggtagt gtcaagatga cactcatttt tactggagct
     7261 gactgctggc tggtcatctt tcggtcaccg acgatccaaa aacgtgtctg gttcaactca
     7321 ttatcttgga tattctcagc taagatctcc aatccaacat gctgtgctgt ttctaatgaa
     7381 gcaatcgctg ctgcgtcttc tttcggatgt tctgctacat acattgctgc agcagttgtt
     7441 gattctgttg ctactaaagg aacattagga taatgcgttt ctaaaaactg ttgtgattgt
     7501 gctagtgctt gtggatgtga aaggattttt gtaatctttg ttgctggatt gcctaaaagc
     7561 tgctgcttga taggaagaac gatttcactt ttaacttcga cttctggatg tttggaaaga
     7621 agatcgattg tgtgatgaac agctccttcc aatgaattct ctacaggaac gacagccaga
     7681 tcgacttgtt tgcgaaacaa agcgcgcaaa caaacaggga tggaagcata cgcagtcaat
     7741 tgttctgtag gaaatagctg acaagccgcc tgataagtga atgaactttc tggccctaga
     7801 tagctgacgt tcatatgctt ttgatccttt ctataatggt atcgatgatc ttttctggtg
     7861 tttgattggt cgtgttgatt cgatgtgtcg cactttcttc ataccaagaa agtcgttgtg
     7921 ccaataatgc ttttatttct tctcttgttt ttccaagagc taaaggtcga atagacgttt
     7981 catcttgttg gatacgatca actagacagt ccgcttctgc ctctaagtaa agaacaatcg
     8041 gctgtgtttt taaaaaacgc cgattttctg cactttggac aatccctcca ccagtcgcga
     8101 taattttgtc ttcatttgac agctttctta aaatatctga ctccactttt cgaaagcttt
     8161 tttcaccgta atagtgaaaa tattcggcaa tcgacaattg gatatgttcc tcaatctttg
     8221 tatctaaatc tacatactct ttttgtaaac attgagctaa ccctttaccg attgttgttt
     8281 ttccagcgcc catgaaacca atcagcaaaa tgctagccat gagcttctcc tcctgacaca
     8341 agttctgcta aatcgtcaaa aaatgccgga taagagattg aaacagcttc agctccttct
     8401 agttcgcaag gtccatcagc tatcaaagca gctacttgca gcatcatacc gattcgatga
     8461 tcgcctctgc tggacacttt tgcgccatgc agaggtgtcg gcccatggat gatcagaccg
     8521 tctgctgtcg cttttatctt tgctcccatt ttctgtaatt cttctgccac cgcatcaatt
     8581 ctgttggttt cttttacttt taattcttct gcgtctttga tgatcgtgat tccttctgct
     8641 tgagtagcca ctaaagctag aatgggcaat tcatcaatca gacgcggaat gatttctcca
     8701 ccaatcgttg ctttcttcaa atgactcgtc ttgacactta aatcagccga ttggttctgt
     8761 tcattgtgat tcgtctgggt aatcgctgcg cccatctcct ccaacacatc taagatacca
     8821 gttcttgttg gattgacccc gacatttttt aacaacagtt ggctttccgg aacaagaagt
     8881 cctgctgcta gaaagaaagc agctgatgaa atatcacctg gaacagttat ctgttgacca
     8941 gttaattttt gcggtccggt cactatgatc gttttatctt ccactgtgat ccttccgcca
     9001 aattgacgaa tcatttcttc cgtatggtta cgggaacgtt ctttttcaac gattacggat
     9061 gtgccttctg cttgtaatgc cgcaaacagc aaagcagatt tgacttgcgc actggctaca
     9121 ggcatattgt atcggatagg tgataaagag cgggttcctg tcacagtgat tggtagatac
     9181 tggtcatttt cttctccttg caagtcagcg cccatctcgc gtaaaggttc catcactcgc
     9241 cccattggac gtttagacaa cgaagcatcc ccgaacagag tagtcgtaaa aggctgtcca
     9301 gccaaaatac ccatcaataa acgtatggtc gtaccggagt ttcccatgtc aattgattcg
     9361 gttggttgga caaatgagtg gctaccgcga ccatgaatct tgatcacctc ttcctcttct
     9421 tcgatctcga cacctaattg ttggaaggca tgtaaagtac tcagacaatc ttctgcgcgc
     9481 aaaaaatttt gtatagtcgt cgttccttca gcaattgctc caaacatgat gctgcgatgg
     9541 gaaattgatt tatctgctgg tatcctaaca gtccctctta atccatgtat ttgttgtaat
     9601 aattgcattt atccagcctc ctatttgatc cagcaatgat aactggtatt agacataatg
     9661 attctttttg cttgtaacaa atcctgttgg tttttaaaac tgatttgcag tattcctaaa
     9721 atatcttccc tggtctcttg gatcttcaaa ttaatcaaag aaactcccgc ttttccgatc
     9781 agtccagtta cttctgcaat aacgccaggt tcatctggca catcgataaa taaatcaaaa
     9841 aaggcaggaa tcgcgccttg cttatggaca ggcaactggt ctcgactttc ttttgcccga
     9901 ttgaaaaaag aaaagatttc cgattggttc tcagtcatga tccattctgc tatttgtgtc
     9961 atttcttgtt gccaatctgc caatcgttcc aataatatct gcttattgct caacaaaata
    10021 tctgtccaca tgacaggatc tgaggaagcg atcctagtga tatctcgaaa gcctcctgct
    10081 gccagttgtt tggctcgcgg atactcttga ttgaattgtt ctgcctgatt gactaagcca
    10141 gctgccagaa tatgtggaaa atgacttagc attcctgtta tacggtcatg ttctcttgct
    10201 gtaagagtta catattttgc tttggttccg caaaatagtt cttgcagttt ttcaacatct
    10261 tttttcgtct tttccaaagg tgtaaagatg taataggcat tttcaaatag tttagcatct
    10321 gctgcctgta tacctgattt atgagaacca gccattggat gtccaccgac aaaccgaaga
    10381 tttttctgct cagcggatgt cacgacttgc tgcttcgtac tgctgacatc cgtaatgatg
    10441 acgtgatcag acaagttgat tctttcaagg acatcaagat aagctaaggt tgtacggacg
    10501 ggaacggcta ataagataac gtctgcttgt tctgcacctg tttcaaaatc acttgcaacg
    10561 taatcaacga tctgctgttc tactgcttgc tcacgggttt ttgactgact atcccaacca
    10621 actactatca gttctggatt ggtctgacga atacaaagtg ccatagaagc accaatcagt
    10681 ccaagcccca cgataaaaac atgttgtgcc atcttatttg cccccttttt agtaatgttt
    10741 caagtagtca cggtactcct tcactgcagc tttcaactca acaagcgaat cacttgggaa
    10801 ttttgacaat agctctgtcg ctaccacagt ggctacaaca ttttccgcaa cgacgctagc
    10861 tgcaggaact gctgtactat ccgaacgttc tacacttgct ttgtaaggtt cttttgtatc
    10921 gatatcaaca ctttgaagtg gcttgtacaa tgtaggaatg ggtttcatga ccccgcgaat
    10981 gacaagtggt tcgccattgg tcattccgcc ttcgaagccg cctagattat tggtacgtcg
    11041 gaaatagcct ctttcttctt cccataggat ctcatccatc acttctgagc ctggtaatga
    11101 accagaagtg aatccgaccc caaattctac gcctttaaag gcattgatac taaccacagc
    11161 ttgtgcgatt ttggcatcta gcttcgtgtc ccattgtacg tagcttccta atccagctgg
    11221 aacgcctcca acgagcactt ctacaattcc tccgatcgta tctccatttt tctttgtctg
    11281 atcgatcaaa tcttttattc tttgctcaac tgctggatca acaactcgta cttctgacgc
    11341 ttcagaaatt tctttgattt cttgaatcga gatatttcca ggtatttctg cttggattcc
    11401 accaagacaa atcacgtgtc ctgctacgat gatgtcaagt tcttccagaa ttttcttggc
    11461 aattgcgccg attgctacac gcatggtcgt ctctcttgca gaggaccgtt caagcacatt
    11521 ccgaagatct cgatgatggt atttcatacc gcctaccaaa tccgcgtgtc ctggacgcgg
    11581 acgtgcaact cgccgcagtg attggtcttt ttcaggcact tctgctacag acatgacagt
    11641 tgtccaattt ttccaatctt tattttctac cactaaagtg acaggtgaac caattgtttt
    11701 gccgtgacgg atgccagacg tgatccgaac acgatctttt tcgatcttca tccttcctcc
    11761 tctgccgtaa ccagcttgtc tgcgagcgag ttcgtgattg atttcttctt ctgacagagg
    11821 taatcctgct ggtagtcctt caataatggc tgttaattct ggtccatggg attctcccgc
    11881 agtcatataa cgcatcttac agtcctccta agtaatcttt gaatgtctct gtgggaacag
    11941 gaacgatttt tgcttgccca atcttttcta gcaagatgat attcaattgg cttcctcttg
    12001 ctttcttatc atgggttatc gcgtggaata attcatctgg atgacttaga tcgtacgtaa
    12061 tcggcagatg gaatttttga agtatccttt ctaaagcagc tgtcgatcca gttggagaca
    12121 agccgatttt ttctgcatga gaagtgatgg ctaccattcc aatagctact ccttctccat
    12181 gactgactac cccatatcct gctgtatttt caattgcatg gccaattgta tgaccaaaat
    12241 ttaaaataag tcgatttcct tggtcaaaga catcttcttc cactactttt cgtttgattt
    12301 ccaacgtagc agtaatgatt ggaacagcat gttccagaag atctttttcg tctttcagct
    12361 cgttcagctg tttccaaagc gttgcatctg ctatagctgc tgatttgatg atttcagcga
    12421 ttccttctcg taaacgacgt accggcaaag tttttagaac ctcaggatca atcaatacac
    12481 cagctggctg gctaaatgtt ccgacaaggt ttttcgcttt gtttgtattg actgctgtct
    12541 tcccgccaat actagaatct acttgtgcaa gtagcgtagt cggaatttga acgaaatgga
    12601 tcccacgcat gaacgtcgag gccacgaatg ctgctagatc acccaccatt cctccaccaa
    12661 gtgcaagtat cccatcgctg cgggtaaaat cattatctgc taaaaaatcg tataactcag
    12721 ctgcttgttc taaagattta ctggtctcac ccgccggtac gattgaaagg actggttcaa
    12781 aattagcttg tctcaattgg aacatgattt tttctgcata tagtggagca acgtttgtat
    12841 cagtgatcac agctactttt tgaggtctcc aaatcgtttg aagccatgat ccagcctgtt
    12901 ctagtgcgcc tttttcaata tgaatatcgt atttttggtt atcaacatgt acagtaagca
    12961 ttcgtttcac ttctttcttt taaaaagctg gaattaatcg agtgtctgaa tcttttccat
    13021 tgcttctttc atgatcgcaa cttctttcat catgcgtgag taagtctttt cattcaacga
    13081 ttggggaccg tctgaccaag catttgcagg atcaggatgg atctctacga tcatgccatc
    13141 tgctccgctt gccacacccg cacgagccat tggtggtaca agttcccaaa ttcctgtacc
    13201 atgacttgga tcaacaatca cagggaaatg agttaatttt ttcatcaatg ggacagcact
    13261 taaatcaaac gtattacgtg tcgctgtttc gtaagtccga atcccacgtt cgataaaaat
    13321 gacaggagat ttgtcttcga ctgcaatgta ttctgcagca ttaagccatt cattgatcgt
    13381 accggaaatt ccacgtttca atccaacagg tttacctgtt ttaccgacag cagatagcag
    13441 tttaaaattt tgcatattac gtgcgccaat ctgtaggata tcgctgtact cagccaccat
    13501 atcgatatgc ccttcatcca tcacttcagt aatcactttc attccaaatt catcagccgc
    13561 ttgacggatg tatttcaatc cttcttcttc taaaccttgg aaagcatatg gagaagtacg
    13621 tggtttgaag gcaccacctc gcagaatttt tgcgcccccg gctttggcca ttcgcgcaca
    13681 ttctctgatt tggtctaatc cttcgataga acaaggacca gccatcgtca cgaaactgcc
    13741 gtcaccaatt tttactccgt ctacatccac gactgtattg ccaggatgga attctcgaga
    13801 agtcaatttg tatgtgttta aaatacgcac agcattttct actccttcat agcgtgtaaa
    13861 tgctccttct tgtaaacttc gcgtatcacc ttttagtcca atcactactc gttctgttcc
    13921 ttgatcgatt gagactttga atccttcttt ttcgatatga tcgacgactt tttttatttg
    13981 ttcttccgtt gcttcttttt tcataattac tatcattgtt tttcccgctt tcttttgttt
    14041 acttatctat cttggctgat tttctctact ttttcaaggt ggttttacat taattcctac
    14101 taaatgaccg tgtcctgttg ttctcatttt cgctccttct ttcctactta tgacccagat
    14161 aatcaacaaa aaaagatgtc cctgtaggaa attgcctcta cagtgaacat cttcaacgag
    14221 ccttaaaaag cgctgcaaaa atataaaata gccactatag atgaatttct ctacgtggct
    14281 gtaagtcgtg tttaaaaatc tttagccatc atagatacaa acctggtttg tttgtaccca
    14341 cgaagtcatg aatacaaacc tatctaaaat agctaaagta atacgtattc agttttgtaa
    14401 gttttctaga tttcataaaa aatcgctcca aactgatgta agaattgtac ctagtataga
    14461 cataaaaaaa tcgactgtca atcattttta tgaaactttt tttaattttc tgatagttaa
    14521 ctttgttttt cagaaaaata acaattggtt ttattgaaga aaatagtcat gaacaaaagt
    14581 atctgagaac ttttttactc tcttgttcat gacatataga ataaagcagc tttgtcttgt
    14641 aaactccgtt tttcaaataa taatctaaag attgctactg aataataaag ccgatttcat
    14701 ggtcctcttc gctgctgatt cggtaaggtg actcaacatc cgctccccag ctattgatac
    14761 ccccgactcc tcttactttt gcaaaaatcg taagaaccgt tcgtctttct ggcggtagtt
    14821 cttctatatg tgttgcctgt tccagttctt ctgctttgaa tggtaaagcg gaaaaagcaa
    14881 atggcgtatc ctgctgttca aatcgcaacg aaaaatcttc ttgatcttta tccgtattat
    14941 ccaacgtttg atttcttgtt agttctaccc atttcgtttc catatgcatg ccgcattctt
    15001 ggggaacaag gtatggggaa acggggagtc ctgccacttt ataaatacct tcgacaccac
    15061 cagccatccg atccggatat gtttcccctg ataggccttc atactcaaaa aattttgctt
    15121 tcgttggcat aatgaacctt aaacccaatg cgggtaattc cggtaatcct ttttgtccat
    15181 aataatgcat attgacaagc attttaccct ctccagcgat ttggtaggaa attgtcactt
    15241 gggtttttgg tactgtgttg atttccagtg taaaaatgat ttcaaccgtt tcagcgtatt
    15301 cattacttga atattgatta tttactggag caatcggcaa atcgatcttt ttattgtcca
    15361 ccagtacttc tatttctttg acttttggat acaatccggc tcctaaccac ataccggatt
    15421 taaatccaaa tccgctacct ctgtcattat cggtcgttgc tcgccaaaaa actggaagtg
    15481 tttcccggta tagccattct tttccgtttt tattgagcga ttccagtccg cctcttgtat
    15541 aagaaaaaat ataatgcgta ttccccgatt tgacacctaa tgtacagtcg ccgaagacga
    15601 ttgcaacttt ttccgtatcc atatgacttt tatttactgt atttttcata ataatatctt
    15661 acctcctgca tggcgggttt ttcttgtcca tctgcaaaaa cgatcccatt ccctgagaat
    15721 tcatagtcac ttggtttatc gtcaaagtca ccgccatagc gcatcacttc tctgcctgtg
    15781 attggatcag tcacttggat cgcctgatct ttaaaatccc atatatagcc tccttggtac
    15841 atttcataac gatcaagcaa cgtcatgtaa gaatccattc cacctaacga attccccatg
    15901 tcatgcatat attcacataa gatgaacggt ttttccgctt tattttcaag gtagtcaatg
    15961 atctcttctg gtggcgcata catgcagctt tttacatctg aaatcgaatc ttcgaatgct
    16021 ggatttggaa aattcccttc ataatggaca agtcgatcgg ggtctacttt tttgaagtaa
    16081 tcgttcattt gtcgaatgtt ttcttccgca taagattcat ttccaagaga ccaaaaaaga
    16141 atacttggat ggttttttaa tgtttcgaaa ttcgtttttg ctcgatcaag aacagccgcc
    16201 tgccactgag gtactgcacc tggtacgttg taagaaggct caaatgcgcc cattttttgc
    16261 catgagccat gggattcgag gttcgtttcc gccatcacat ggattcctgc ttggtcgcat
    16321 aaataatacc aagtgatctg gttggggtaa tgagatgtcc gtacgccgtt gatctttgca
    16381 ttttttatga tagccaaatc tttttccata tcttctatag tgatggcgcg tccttttttc
    16441 ggatgccact catgtcgatt taccccgttg ataatcatcc gttttccatt gaagtagatg
    16501 attttatctt tgatttcgat tttacgaaaa ccgaaggaat agtcaatata ctccgttact
    16561 cttttttctt cgtctatcgt ctccaattca agcttatata aattgggctg aaaatgcccc
    16621 cacagtgcca cattttcaaa tccatcgatg cgaatcgtca tttgctcttt taacggatat
    16681 tgctgttgat agatttcctt cccatcagga tctagtatcg ctacattcag ctgactgttt
    16741 ttggctactt gctcaaaaga caaatctact tcaagtttgc cattttgaca tttgtcatct
    16801 aagcgaggtt tgatccaaag atcttctaaa tggttttgtc ctcgagcaac taagaagaca
    16861 tctctgaaga tcccaaaaaa tcggaaaaag tcctgatctt ccaagaatgc agctgtactt
    16921 cttttgtaaa cctccacagc tagaatattt tcttcttcca aataaggtgt taaatcaaat
    16981 tcagacggag tgaacgaatc ttcggcatac ccgataaaat gaccattcaa ccaaataaac
    17041 attgcttgtt ctacaccagc aaaatgaatc gagatttgtt ttccctttag accttcttct
    17101 aatcgaaaag tcgtcaaata cgaaccaacc ggattgtctt tagcagctga aaagatccct
    17161 tccgcctctt tctcagggct tgtcgtgaaa gggggacggc gaaatacatg tccctcccac
    17221 ggatacatcg tattgatgta atggatcttg tcataatttg ctaattcaat atgactagga
    17281 acagtgattt catcaaaaga acaggcatca aaatcttttt tataaaatgt ttttggtcgt
    17341 accgaaggat ttggcgaata acaaaaacgc catgatccat ttaaagactg tttaagggag
    17401 gatgtggttt ctacttcttc ccttgttgca taacaaacat gatcgctatg tgcatttgat
    17461 tggttcactc taaaaacttg tggatcatct aaccatttta tatttgcttt catacttttt
    17521 tccttctctc tcataacttt ttgaaatgaa gcagttctgt ttggaaatca ccttgaaaat
    17581 cggggaagta aagtccaact gtcatcaatt cggcccccga aaatatccgc ttcgaggtct
    17641 gttcctgata aagtgcctca tcatctaacc ccatcagata tacttcatgg aacgctggtt
    17701 gagctgaagc tagtatcctc cctaaaaaca caatagcttc atcttgttta ggagacacaa
    17761 acatccatgc ggcaatattt tcttcaaaag gactcgccaa acgataataa tggccatact
    17821 ggatcaccgg acgaatcgtt tgataggata taatttggtc ttttacttgc tttttttctt
    17881 caggagacag ctttgtcaaa tccagttcat aaccaaaaac agcgctcata gctgtttctc
    17941 ctcttgtttt caacgaagta gatctgtcgg tctgatgatt tggtacagct gatacatgag
    18001 ctgtgatgga cgaaatcgga taagcaaggc ttgttccata ttggattttc atacgctcca
    18061 ctgcatccgt gttatcactt gtccatgact gcggcatata ataaataaac ccggcatcaa
    18121 accgtccacc tccaccagaa cagccttccc ataagatttc cggataatca gttgtcaaat
    18181 gttcaagtag ttcataaagc ccgagcatat aacgatgcgc tacttctcct tgtctctgtg
    18241 ggtcaagggt aatcgaatag acatctgaaa gcgaacggtt catatcccat ttgatgtagt
    18301 caagaggaat cgtatctaaa atctttctca tttgtttctc aatcgtctgc cgaacatcta
    18361 accttgtaaa atccagtaca tgttgtgatc tggaagcaga aggcattcta cctggctcct
    18421 gcataagaaa atcaggatgt gttcgataca gttcagaatc aaccgagatc atttcaggtt
    18481 caaaccataa accaaatttc aatcctttgc tatgagcata atctgctatt tcacgtaatc
    18541 catttgtcaa cttgccttca tactcgaacc agtctcctaa tgaagaattg tccgcatctc
    18601 tttttccaaa ccagccatca tctaatacga acatttcgat gcctagctcc tttgcttcat
    18661 caatgatcgc cttgattttt tcactattga agtcaaagta agtggcttcc cagttattga
    18721 ccagaatcgg ccgttcagca aattgatgac gacctcttgc gattctttca cgtagcaact
    18781 catggtggat ctgactcatt ttgttcaatc cctgacttga gtaagaaagg atcatttcag
    18841 gtgtttgaaa agactggcct gcattcaatt cccacgaaaa acgatggctg ttgatcccac
    18901 cgacgactct caattgattg atctgatctt tttcaagttc gaaagaatgg ttgccggaat
    18961 aaacaaattg aagtccgatc gcttctccag tattttctgt cgtatgatga tgaactaaag
    19021 caataaagtt attcatatgg tgactgctcg ttcctcgacg gctttcaaat acttttgttc
    19081 cagataaaac agattgccgt gaaatctgtc tttcattgac atgtgctcct ggcaaagcaa
    19141 tgacttcatc aaatcgccta gtatgggcaa agtctagctg gaaactagcc gctttttcta
    19201 gtttgattgt ttctccggtt tcatttctta tctttacaga tcgtgtgatc actgctctat
    19261 cagtaaatat ggtatagaac aaatcgaaat aaatcgctgt ttctctatcc ttaagtgaaa
    19321 tcaccaacgt ttgtgcctct gacttgtcca tcacataagt ttgaggcaag ccttcgatct
    19381 ctggcttccc atctgtaata taatggctgt catatctcag atccagtaag tctgatccat
    19441 tcttgcgctg aatgatcgat gcgggcgttc gaaagtccat gctgccatga gaagagtatt
    19501 cttgcggtag tgtatctttc gacagagacc ggtcaggatt taagggaaca tttcctgaaa
    19561 atccacgatc tcttcgggga taatttttat tgtctttgta ctgcttgact ggtttgccaa
    19621 aataaatatg cgccaataca ttgctttctt ctatctttat cagataagaa agaaatgtat
    19681 ttcgtaaatg gaagactcgg ctcttttcat caaataagat gaaattttta tctgtttttc
    19741 tatgcgtaat atccatcatt tcactcccaa caaaacggaa accgtttact tttttttata
    19801 attcctgtat actgtttact aaacaatttt gtcaataaaa attttttatt tttttgagga
    19861 gtgtgtaaaa atggttggaa ttagagatgt cgcacgaaag gcaaatgtct ctcctgctac
    19921 agtttcccgt gtgttgaata acgatcagac gatccatgtg gctcagacta ctcggcaacg
    19981 gatagtagag gcagctgctg cgttggatta tgacatcagt actcgtaaat atatcaaaaa
    20041 gagaatgcct tcgattggtg tgatctctac gatcggcaaa gcgagcgaag aacaggatat
    20101 ctattacaaa catttgcgag tagggatgga agaagaagct cgacgtctgc atttagggat
    20161 gaatcgaatc tataatctat ctgataatcc taaacaatgg gaagatctag atcagttagg
    20221 tgcggtgatc gttgttggga ctgttacgaa agaatcgatt accgccttgt taaaacaaaa
    20281 catgcaaata gtcgtcgtgg ataaccctga catccagcaa gaggtcgata tggtgtatgc
    20341 agatcttgaa cggatgacta aaaccgtact ggaattgttc ttagaaaatg gtcataaaag
    20401 aatagcctat atcgggggat ataaagtcga tatggatgaa ttcggtggga agcagatttc
    20461 tataaatgaa aagaggatgc gtgcctatca gcattttatg atcgatcatc acttagaaaa
    20521 atatatgtct tatcatctag gtggctggac agaaaaacaa ggtgaggtac ttgcgactaa
    20581 tctattagaa aatgaatccc ctcttcctac tgccattctt gtcgggagcg atccaatggc
    20641 aattggtgtc tatcgggcat tgaaacaaaa aaatatccaa attggagaag agattgttgt
    20701 agcaagcttt gataatatcg agacaatcgc aaacctctcc cctggcttga ccagtgttca
    20761 aatcaatgcg aaatcaattg ggcaagcagc tgttcgaatg gcattggagc gaattgacgg
    20821 tatacgggac gaaccaatca ttttgactta tcctgcacga ttgattgtta gggaaagttt
    20881 tatcccaaga acaaaaaggt attgaaagag aggttgtgaa aagccgcttc gtttcttcat
    20941 actcaaaacg aaacggcttt ttcacaacct cttacttata cttattcaca aaatccaatg
    21001 tctgctggta acaattttgc gattctttta gcgtgtaatc aaattggtat tcatgggtaa
    21061 tctctttttt tgtatctgta taaaacaagg attgcacggg tacctttaat tctgtcaacc
    21121 gattttctaa tgcttgacct tgctcctgga atgagtatgc atttccgtca gttatatacg
    21181 ttggcggaaa ttccttagaa acatggtcag ccacactagc ttctttcaat tgctcactcg
    21241 ttttccaatc ttttgttcca atcaacgacc acgcaacggt tttgacgaaa aacttcataa
    21301 atcgatcatc cgaatgctgc tgtgctgtta gcttcaaatc aacaggtccg caataagaaa
    21361 tggttccttt cagactatct tttggaagta gctgtttgat tttcatttgt ttagcatacg
    21421 catcattcgt ttgtatagct gcatattgca atgcgatttg tgaacctgca ctatctccac
    21481 caaaaaataa ttgttccatg tctagcaatt tgtcgtcttt ttcttcagct aataaatctt
    21541 tgatcagttc attcacttga agaacttgat tagggtattc tgaatcaggc gctaattcat
    21601 aattcatcgc aaccactgcg atgttggcgt ctgaaactaa ttttgtcgca aattctttga
    21661 cacccgattt atctccgccg acgaatcctc caccatgtac ccaaaataga acaggaacag
    21721 gccccttgct gttttttgga tagtagatgt cataagtatt ctctttatac ttggactcat
    21781 acgtcaaatc tgtttttttc gccacgtttt tttctgcttg atcgaacgtt tttttatcag
    21841 tgatttgaac agtatttgaa aacatatggt tgatcaagaa tgctccaggt ctgggggaaa
    21901 gctggaaggc aagaaagatt gcaaacgcga tcccaagaat gattccgaag aaccatccga
    21961 taaatttaag aattttcttt ttcataaggc tccctcagta gttagtatta agcattctga
    22021 ttataccata gtgttatttc ctatcaagct ctcctagatg atttaatgat tttccccaaa
    22081 cttcttctgc aatgcttttg acaaaagaaa ttttttccat tgttcttcaa acaaaacatt
    22141 ttcttcttct gttgaagcaa atccacattg aggacttaag cacaaacgat ccaaaggcag
    22201 atatcgtgtc gcttcgtaga tccggtcaat cacttgctgg tgctcttcca tttcgccatt
    22261 tttcgttaca gtcaaaatct aagacaacta atttatcttc agagactttc tctaaaagaa
    22321 caaaactccc agcacgttca ctatcgtatt ctaaataata ggcacggaca ttttcttgag
    22381 taaaataatg ggtctgctac gtcgtcatcc cccctgatgc cgccaaaatc gaataataat
    22441 ttccgcgtca aacatgtata tttagtgttg aatcatttga gaatatgaaa ataaaaatct
    22501 ttttgatcaa tcagttaatc aaattgaaat aacgaaaaat ctgagacaga atgacgttca
    22561 ttctgaccca gatttgattt tgcacaactc aacagtcctt attggcaaat cgccaaaata
    22621 atgtagacta actaaaaaga ctaagaaaca ataacaaaag ttaacacttc ttagtctttc
    22681 tttagcacgt ctatttatta aagattttag ggccttttct catgttttta cgatcattct
    22741 taggtgtttc catttgtttg gatcggcctc caccttgttt ctttttgatc aattctttca
    22801 ttttttcttt ttggtccatt tttattctcc tttaacgaat gaagcgacta attcgatatg
    22861 atgggtttgc gggaaaagat ctacaggctg aactttcttc acggcataac ctgcttctgc
    22921 aaataactta gcatctcttg caaaagttgc cggattacat gagatataga cgattcgttc
    22981 tggtgacatt tcgattgctg attcaataaa atgatgatct aatccttttc gtggagggtc
    23041 aactacgatc acagatggtt tgatcccttt ttcttgccat tttttcataa ctttttctgc
    23101 ttttccgact tcataatgaa cattttcgat tttattattt aaagcattga actgtgcatc
    23161 ttcaattgct tctggaataa cttccatccc atacacctta tccactctgt ctgctaaaga
    23221 caagccaatc gtacctattc ctgaataagc gtcgatcaca atgtcttttt tatctaactc
    23281 ggcaaactcg atggctgttt ggtacagata ctctgcttgt tgagtattta cttgataaaa
    23341 tgactttgct gagattcgat aggtttttcc taatagctga tcttcgatat agtctctgcc
    23401 atataaaact ttttctttgt ctcctaaaat cacatttgtt ttttcttcat tgatattttg
    23461 gatgaccgaa accacttctg gcaattcttc atgaatagct tcagcaattt ccttggattt
    23521 aaagaatttt tctttacgag taactaaaac gaccatcatt tcatgggaat aatgaccacg
    23581 acggataatg atgtgacgaa gctgtccttc atttttttct tcattatatc ctcgtacttg
    23641 gaaccgttgc aagatatctc tgactacttt gattgcttga tcgatgacag gatcttggat
    23701 ataaaaatct tcgataggga ctagatcatg gctattcttt ttgtaaaaac cggtggctaa
    23761 ctgtccatcg atacggcgaa cagggatctg tgctttatta cggtagccga atggttctgc
    23821 cattccgacc gttggaagta caggaacttc tggcatctta gcaatcttcg ccattacatg
    23881 ctctacttgt ttttgtttga agacaagctg ttgttcatac ttcaaatggg ctaaaggtgc
    23941 gatacctatg cgaagtaatg cattgttttc tacaggctga cgatcaggtg attgtgtaat
    24001 aaatgtttcg acttttccat atccaaactt attgcctgct tttactacta gtatttctac
    24061 ttcttctcca gggagtgcat tttcgataaa taatggataa ccatcgattt ttgctacccc
    24121 cattccttca taactcaagt cgacgatttc gactgtatat cgttcgtttt tctttactat
    24181 ttgctgttct gtcatatttt gctcctttaa gcctattttt caatcctact gaactctact
    24241 ataaatcagc taattacaca actaaaaccg gcactgacct tactacgttc agcaccggct
    24301 tttgattaat tcttttgatt aggtgtttcg tctgctattt ttccgtcacc gtcgatatct
    24361 tcatccgtta aacgttcgac ttcttttacg aattctttgc tgacaacctc taattcttct
    24421 tcatctgtac ctgtgattgc ttcatcagga atctgatcgg tattggcaaa gaactcaatg
    24481 tgttgatgaa gattttggaa atgcatcggt gcttccccgc cgtattcacc atctaagttg
    24541 atcattactg gtcgattgat atcatcttct gtttcaacga acaaactgct tgttttggta
    24601 tagattattc ttggatcatc gacatgtcga ccgccattca gcatgagtgc tgcaatatgc
    24661 aagatctcaa agatatttgc tgtcttcaca atgattaaag aaaacttccc atcatccaat
    24721 ttggcatctg gcgcgatctg ttcgaatcct ccaacagagt tcgtcagccc taggaaaaac
    24781 atcgaagcat ttccgatata ttccccttca tcatacttca agcgcatttt gattggcttt
    24841 actcgaggga gcatctctgc tcctttcgca agataagcaa gataaccaaa aatactcttt
    24901 agttctgatg gaacttcata agtcaattct gtcaaatagc cacctgcagc gatattgatg
    24961 aagtaactgt tatcagaaag gccgatgtcc atttttacgg tttggtgttt ttttatcact
    25021 tctgctgctg ctttaatatt atctctagga atttttaatg ctcgagcata gtcgttcgtt
    25081 gtaccagctg ggatgatggc cattttagga cggtgttcaa gcggagcgat tccattaacc
    25141 acttcgttga ttgtgccatc gccaccagct gccactacta gatcaaaacc tagttctgcg
    25201 actctacatg cttcattctt ggctgaatct ggttctggtg tagttgcata ggcgctggct
    25261 tcaaatcctg cttcttctaa tgcctgtagg atatccgcca aatttttttt caataattct
    25321 tttcccgagg tggggttata aattacgcgt gcttttttca ttttcagcct ctatcgtttc
    25381 gctaattcat ctttaagtag cttattcacg acaccaggat ttgcttgtcc tttagttgct
    25441 ttcatgattt gaccgactag gaagccaacc gcacgatctt ttccattttt gaaatcatcg
    25501 acagattgtt ggttgttatc caaaatttca ttaatgattg gcagtagttt gttcggatca
    25561 gataattgca cccaaccatt tttttcaact acttcttggg cttctccgcc atttgtgatc
    25621 aattcacgga agactttttt ggcgattttt gagctgatcg ttccatcggc gatcaaacga
    25681 atcattcctg ctaagttctc aggtgtcaat tttgtctcag ctaattccag tttctcactg
    25741 ttcaagtaag cagaaacttc acccatcaac cagttagatg cttgcttggc atctgcacca
    25801 ttttccaaaa cagcttcaaa aaaatcagac atttcttttg taagtgtcaa taccattgcg
    25861 tcatactctg gaagtccaaa ctcacggata tagcgttcgc gacgagaagc tggcatctca
    25921 ggcaaactag cttttacttt ttgaatccat tcgtcatcga tcacgaattt cgggatatca
    25981 ggctctggga agtaacgata atcactagac ccttctttga cacgcatcaa aactgtttta
    26041 ttcgttgttt cgtcaaaacg gcgtgtttct tgttggattt ctcctcctga aagaagaact
    26101 tttgcttgac gtttttcttc aaaagctaaa ccttttttca cgaaagataa agagttcaag
    26161 tttttcaatt ctgctttcgt accgaactct tcttgtccat aagggcgaag agaaatattg
    26221 gcatcacagc gcatagagcc ttcttccatt ttcacatcgc tgacttctgt aaattggata
    26281 attgatcgca atgcttctag ataagcgtat gcttcttctg gtgaacgcat atcagcttca
    26341 gaaacgattt cgatcaaagg cgtgccttgt cggttcaaat caacgtaaga ataaccgcca
    26401 ataccatgca tgttttttcc agcatcttct tctaaatgga cacgttcgat ccggattttt
    26461 ttctttttac cttctacttc gatttcgatc catccgtcat gaccgattgg ttgatcaaat
    26521 tgggaaattt gatatgcttt tggattatct gggtaaaagt aatttttccg gtcaaaatga
    26581 gtatctttag agatttcaca gtttaatgcc aaagctgctt tcatcccgaa ttccagcgca
    26641 gctttattca ttactggcag cacgcctgga taaccccagt cgatcacgtt tgtattgctg
    26701 tttggttctg caccaaaatg agctggcgct ggagagaaaa tttttgagtt ggtttttaac
    26761 tcgacgtgga cttctagtcc aatgactgtt tcaaaattca ttcgtctttc cctcctaaaa
    26821 tagctggttg ttgtttatgg aattctgttg cttgttcaaa cgcatatgcc gcttgataca
    26881 tcgttttttc atcaaaataa ttaccgatga tctgcaatcc aactggtaat ccttctgata
    26941 aacctgccgg tacagacata cctggaagtc cagccaagtt gacagggatc gttaagatgt
    27001 cactcatgta cattgtgatt ggatcgttaa tgttctcacc gattccgaat gcaactgttg
    27061 gtgaagatgg cccgatgatc aagtcataat ctgcaaagac tttttcaaaa tctcgtttga
    27121 tcaatgtacg gacttgtcct gcttttttga agtaagcgtc ataataaccc gcgcttaagg
    27181 agaatgtacc taacatgata cggcgtttta cttcttcacc gaatccttct gaacgtgagt
    27241 tgacatatac atcatctaat gatttcacat tttcagagcg ataaccgtag cggataccat
    27301 cgaatcgttg caaattggaa cttgcttctg atgaggcaat gatataataa acagccacac
    27361 cgtattttga atgaggcaag ctgacttctt ccacaacagc acctaatgaa cggaatgttt
    27421 ctgctgcttt caatacagct tcttttacgc caggggccac accttcgcct aagtattctt
    27481 taggtaaagc aatcttcatt cctttgatgt cgcctgtcag tccctctgca aaatcaggaa
    27541 ctgaaacgcc tgaagaagta ccgtcttttt cgtcataacc gctgatagct gtcaatgcta
    27601 atgcattatc tttcaccgta cgggtcattg gtccgatttg gtcaagagat gaagcaaagg
    27661 caatcaaacc aaaacgtgag acacggccat aagtaggttt catccctacg atgccgttaa
    27721 aagctgctgg ttggcggata cttcccccag tatcactacc taaagaaact ggaatttgtc
    27781 cagctgcaac tgctgctgca gaaccaccag aagagccacc aggtactttt gttgggtccc
    27841 aagcattttt tgttttcttg aaataagaag tttctgtact tccacccata gcaaattcat
    27901 ccatgtttag cttaccaaca gggatcatat ctgcttgata gattttatcc atgaccgttg
    27961 catcataaat tggttcaaaa ttgtaaagga tcttagatgc agcggttgtc aagatatctt
    28021 ttgttacgat attatccttg ataccaattg gaataccagc aaggacgttg ctttcatcaa
    28081 tccctttttc atctaaagct tttgcctgtt ccaatgcttt ttcttcgttc aatgtaatga
    28141 atgcctcgac ctcaggctct gtttctttga ttcgttcaaa tgttgttttt gtcaaatctt
    28201 gtacagtgat ttctttcgat acaagcatat gatgcagttc ttctagagaa cgattgtgta
    28261 attctgtcat tatgcgccag cctccccatt atcgatgatt gcgggtactt tgataaagcc
    28321 gtcttctgtt tccggcacat ttttcaataa ttcatcgcgg ttccatccag cttgcgcccg
    28381 atcttgtcgc atcacgttta ctgtttcaac cacattcgac gtaaatggaa caccttccgt
    28441 atctacttct tccagcattt caaccatatc gatgatttta cctaattggt cggtaaaact
    28501 tgcaagttct tcatctgcga acgcaagttt tgctaatttg gcaacgtgtt tcacttgttc
    28561 ttcactaatc gccatactac cccttctttc taaacgagtt aaaaactttg ctctctattt
    28621 taacacaaga gtgccgtgtc tgaaattgtt tattgaaatt tgtctgaaaa aactcatttt
    28681 tcattcttct actgagcgac ttttagccaa tgcatctgcc atttgatctt ctgtccaaac
    28741 ttcgattcct aattcttgtg ctttggtcag cttgctgcca gcatcttctc ctgctaccac
    28801 gatatccgtt tttttcgata cgctaccagt cactttcccg ccaagatttt cgatcgtctc
    28861 ttttgcttct tcacgagtaa atcgtgttag tttaccagta aggactactg ttttttcttt
    28921 aaaaggagac tcgacttctt gcaattgggc atttcgaagc cctttgtatt cgaaattgac
    28981 gcctgcttgt tttaattcat tcatcaattc atggacttct tcgttactga aataagtgac
    29041 tacactgtcg gcaatgatat caccgatcgt atcaagcgca ataatcgact catagtcact
    29101 tttactcaat gtttctagat caccgaaatg ttctgctaaa atttttgctg ctttcgctcc
    29161 gacatgtcgg attcctaacc cgaaaatcaa ccgctcgacc gaattatctt tgctgttgtc
    29221 gattgctgtc aaaatattat ttgctgattt ttcttttatt ttgtccaacg tcaataactc
    29281 ttcttctgtc aatttgtaaa gatcagcaac atctgctact agttttttat catacatctg
    29341 ttctagtacg cgcggcccca acccatcgat attcatcgca tttcttgaaa caaagtggtt
    29401 caaaccttct ttcatttgag cagggcattt aggattgatg caccgtaagg ctacttcttc
    29461 atccaaatgg acaagttcac ttccacaaac tggacaatgt gtaggcaatt ggtattcttc
    29521 gctgtcttta ggtcgtttat ctaacacgac ttgcgaaact tctgggatga tgtctcccgc
    29581 tttataaatc agaactgtat ctttcaagcg gatatccttc aacttaatat aatcaccgtt
    29641 atgcaggctg gcacggctta ctgtcgttcc tgctactcgg actggtgtca tgatagctgt
    29701 tggcgttact acccctgttc gaccgactgt ccattcgatg ttttcgatta gcgtttcaac
    29761 ttcttctggc ggaaacttgt aagctgctgc ccatctcggt gctttgatag taaaaccaag
    29821 ttgatcttgt agcgagaatt cattcacttt gatgacgatt ccatcgattt catagggcaa
    29881 atccacacgt ttgtcatgat attcttcaat gtatgaccac acttcatcaa tcgaatgaca
    29941 tagacgtttt tcccgattcg tgtggaatcc aattttttct agctcttcta gcgcatcata
    30001 ctgggttttt gcttgcatag gaccgaaatc agctactgtg tataaaaaag tgtccagatt
    30061 tcttttcgct gtgattttag aatctaactg acgcaaagaa ccagctgcgg cgttccgagg
    30121 attcgcaaag atatcttttc cctctgcttc tctgtcttgg ttcaattgga caaatgaccg
    30181 ttttggcatg aaacattcgc ctcttacttc gatgttcatt ggttctttta atttgattgg
    30241 gaccgaccgg acagttttta ggttttcagt gatgttttct cctacagttc catccccgcg
    30301 agtcgcacct ctcacgaaat ttccatcttc ataacgcaaa gaaacggaaa gtccatctat
    30361 ttttagttca cagcaatagt caactacacg tcccactgct ttttgtacac gttgatcaaa
    30421 agctatcagc tcttctttgc tgaaaacgtc gttcaaactg taaagcggaa tatcatgagt
    30481 gactttttca aaaccttcta atacttttcc gcctactcgt tgagtgggag aatcagacgt
    30541 gatcaaatca ggatattgtt cctcgattgc tactaattct gcgtattctt tatcataaac
    30601 atagtcttcc actgttggtt tatcttctac gtagtattcg cgagaccatt ggttcagtct
    30661 tgtacgaagt tcttctgctc gtttagcagc ttcgtcaatc gtcatttcat gttccatcat
    30721 ttaccctcca ctactaagct ttttcaattg gcgcaaaggc agccaaaaga cgttttactc
    30781 cttgacttgg gaaagccaca tctagttcta gatccttcga cgtcccgctt actcggacga
    30841 ctgttcctag accccatttt ttgtgtttga ctttatctcc aggtttccag ctgttatttt
    30901 cccctccagt agcactttta gagggaacaa ccgctgtctc tggctgttta tagctatatg
    30961 ctgttttttt agcgaataca ggcgtttttt taggcgtagg acgcatgcct tcgatttcca
    31021 gtaactcctg atcgatttct tctacaaaac gagaaggtcg gttatattgc gtccgtccat
    31081 ataatgttct ggagaaagca tttgttaaat acaacgcttc ttctgctcgc gtgatcccca
    31141 catatgcgag acggcgttct tcctccagtt cgctttcttc catcaatgct cgagataaag
    31201 gaaaaacccc ctcttctaga ccaatcagga atacaactgg aaattctaat cctttggctg
    31261 catgcaaagt catcaatgtc acttgggaag catcttcttc taaattgtca atgtcggaaa
    31321 caagtgccag atcatttaag aagaccgtta gtttttcttc tggtgcatca gcgtcttctt
    31381 cattttgctg ttcgaattgt ttgtcgaatt cttgtgtgac cgttaaaaat tcctccaagt
    31441 tttcgatgcg tgcttgtgct tctaatgtat tttggatttt taagtcttct agatatccgc
    31501 tacgatccaa aacctctttc gtcaattctg tcactgtcaa gtatgggatc atctgagtga
    31561 cttcttggat catctctcca aatgcaccta attgctgtcc tgcttttcca ccgatatttg
    31621 ccagatccac attttgcgct gcttcaagca gtggccattc atgaagagaa gcaaaactgc
    31681 gtagtttttc gatagatcct ggtccgatgc ctcgtttggg cgaattaacg atacgctcaa
    31741 aactgattga atcttgagga tttaccagca cattcaaata cgctaaaata tctttgattt
    31801 ctttacgatc gtagaacttg tgccctccaa ccattttata aggaatattt gctttcaaca
    31861 gagtttcttc cattacacga gattgtgcgt tcgtccggta caagatggca aagtcgccgt
    31921 aattccgatg attgcttcga atctcttctt gcatccggtc tacgataaaa cgggtttcgt
    31981 ctcgttcatt atctgcacgg tagtaagtga tcttgtttcc ttctttgttt tctgtccaca
    32041 agttttttgg ttttcgattg ctattgtttt caatgacttg gtttgctgcg ctaaggatgt
    32101 ttttcgtgga gcgatagttt tgttctagca aaattactgc agcatctgga taatcttttt
    32161 caaaatccaa gatgttctgc atatctgcac cgcgccagcc gtaaatactt tgatctgcat
    32221 ctcccacgac gcacaggttc ctaaatctac ctgccaataa attgactagc gtatactgtg
    32281 cgtgatttgt atcttggtac tcatctacat gaatataatg gaatttgttt tgataatacg
    32341 tgagtgaatc aggatgttct tcaaataatc gaatcgtatt catgatcagg tcgtcaaagt
    32401 ccatacattg attatattga agctcttttt ggtaagcagc ataacatttt gccgcaatct
    32461 cttcgtaata actgccttgc atttttgcaa aatcctgagg ggtctgcagg ctgttttttg
    32521 cttgagagat tgttcctaat atacttcgag gatcatattt tttcggatca atgttcagtt
    32581 cttttagtat ccgtttcatc aaggtcagct gttcagaaga atcgatgatt gtaaagttcc
    32641 ggttataccc gataaaatca acatctcttc ttaaaatacg cacacacatc gagtgaaaag
    32701 tcgataccca gacatcttcg cctccagatg cgagaatcgc attgacacgt tctttcattt
    32761 cttttgcagc cttattggta aaagtaatcg ctaggatgtt ccaaggattg acttcttttt
    32821 cctcaatcaa ataggcaatg cggtgtgtca gcactctcgt ctttccactt cctgcacctg
    32881 ccattagcag taatggtccg tcagtgtgaa gaacagcttc tttctgacgg gggttcatcc
    32941 cgtttaataa ttcagctttg gatgacatta acaacatcct ttcttttatc aatcttgtta
    33001 attatagcat tttcttggct ttcattaaac tagctagttc aaaatcattt gtttcttttt
    33061 atggattttt ccttgtcaat tcgttgattt cgcgctaaat taaaagaaag cgaggcgttt
    33121 gaatggaaaa aaaaggaaaa atcaagaaat tagataagac tcagatatat tttattctaa
    33181 aaggaatatt tgttggactg ctgacaggaa tcgtcgttag cctgtttcga ttatctatcg
    33241 aaaagctaag cgatttagtt cgaagtatct atgaaatgag tcgggaacag ccgatttact
    33301 taattggcct agctatttgc tgcatccttg cagcattttt cgtgggttat ctggtcaaaa
    33361 atgaaccgga tatcaaggga agcgggattc cacaagtaga gggacagctt cgcggggaat
    33421 tatcaatgaa ctggttctct gttttatgga aaaagttcat tggcggtgta ctttcagttg
    33481 gtgcgggcct ttttctaggt agagaaggac cttctataca gcttggcgca tccattggac
    33541 aaggagctgg acaattgttt cggagtccat cttcagaaga aaagatttta atttctagcg
    33601 gtgcaagtag cggacttgca gcagcattca atgctcctat cgcaggatta ctctttgttt
    33661 tagaagaaat ccatcactcc ttttctccac ttgtttggct gacatcattt gcttcagcaa
    33721 tcactgctaa cttcgtctct ctttactttt tcggcttgag acctgtttta tatctaggtg
    33781 accttccttc tttgccgttg aaatattatg gttccttagt gatcttagga gtacttctag
    33841 gcatcttagg ttttatttat cagaatgtct tgctggcttt gccaaaattt tacaaccgat
    33901 tgcctttacc agcttatttt cacgggttcg ttcctttctt gctgattttg ccaattggtt
    33961 atttatggcc tagtactcta ggcggaggca atcaaatcgt tttagctttt ggacatgctt
    34021 ccttgccaat cattatgatt atcggtttgt ttgttcttcg attcgttttt tccatgattt
    34081 cttacggtgg gggtcttcca ggggggattt tccttccgat attgacattg ggggctttaa
    34141 tcggtagctt ttatggaaac attatggttg atttcgggat ggatccaatc tacatcaagg
    34201 attttgtcgt tattgccatg gctggttatt tcacagcgat tggaaaagca ccgctgacag
    34261 caattatttt agtgacagaa atggttggct ctcttaatca tctgatgcct ttaggattgg
    34321 ctgctcttgt tgcttatatc gtcaatgacc ttctcggcgg taatccaatt tacgaatcat
    34381 tattggaacg tctacttgga aatactcaga ccgcctcact aaaaggtgca aggacaactt
    34441 ttgattttcc agtaacggca gagagttctt tagacggatt aatggtacga gacttcaatt
    34501 ggcctaaaaa tatgctgcta atctctatac gcagaggaaa tcaggaaatc ttgactcacg
    34561 gtgatacgat catgaatgta ggagatattt tggttatctt gactgatgaa gcaaacttgc
    34621 caagaatcaa gcaggaaatc gaggctaaat cttctatcac aaatttgaag caatcatttt
    34681 aacgaacatg gaaaaactat tttacaacaa ctattcatct acactacttg attctatatg
    34741 attctataaa aaaagaagag gctgggaaaa
//
LOCUS       DANKEF010000025        34071 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000025 DANKEF010000000
VERSION     DANKEF010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.025"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            23..646
                     /locus_tag="PWZ48_001381"
     CDS             23..646
                     /locus_tag="PWZ48_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261049.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="PWZ48_001382"
     CDS             1096..2145
                     /locus_tag="PWZ48_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2261050.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="PWZ48_001383"
     CDS             2142..3023
                     /locus_tag="PWZ48_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261051.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="PWZ48_001384"
     CDS             3026..3796
                     /locus_tag="PWZ48_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2261052.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="PWZ48_001385"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="PWZ48_001385"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDL2261053.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="PWZ48_001386"
     CDS             5107..6270
                     /locus_tag="PWZ48_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2261054.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="PWZ48_001387"
     CDS             6387..6797
                     /locus_tag="PWZ48_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2261055.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="PWZ48_001388"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="PWZ48_001388"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2261056.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="PWZ48_001389"
     CDS             9370..9654
                     /locus_tag="PWZ48_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261057.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="PWZ48_001390"
     CDS             9814..10050
                     /locus_tag="PWZ48_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2261058.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="PWZ48_001391"
     CDS             10047..10445
                     /locus_tag="PWZ48_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2261059.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="PWZ48_001392"
     CDS             complement(11112..12056)
                     /locus_tag="PWZ48_001392"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HDL2261060.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="PWZ48_001393"
     CDS             complement(12352..13296)
                     /locus_tag="PWZ48_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261061.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="PWZ48_001394"
     CDS             13517..14731
                     /locus_tag="PWZ48_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2261062.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="PWZ48_001395"
     CDS             14753..16768
                     /locus_tag="PWZ48_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HDL2261063.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="PWZ48_001396"
     CDS             complement(16851..18224)
                     /locus_tag="PWZ48_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2261064.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="PWZ48_001397"
     CDS             complement(18250..18534)
                     /locus_tag="PWZ48_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2261065.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="PWZ48_001398"
     CDS             complement(18536..20551)
                     /locus_tag="PWZ48_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HDL2261066.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="PWZ48_001399"
     CDS             complement(20730..21035)
                     /locus_tag="PWZ48_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HDL2261067.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="PWZ48_001400"
     CDS             complement(21124..22155)
                     /locus_tag="PWZ48_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2261068.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="PWZ48_001401"
     CDS             22362..23237
                     /locus_tag="PWZ48_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2261069.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="PWZ48_001402"
     CDS             complement(23389..24231)
                     /locus_tag="PWZ48_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2261070.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="PWZ48_001403"
     CDS             complement(24323..26200)
                     /locus_tag="PWZ48_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2261071.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="PWZ48_001404"
     CDS             complement(26197..27618)
                     /locus_tag="PWZ48_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2261072.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="PWZ48_001405"
     CDS             complement(27621..31010)
                     /locus_tag="PWZ48_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2261073.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="PWZ48_001406"
     CDS             31267..32658
                     /locus_tag="PWZ48_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261074.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="PWZ48_001407"
     CDS             complement(32731..33378)
                     /locus_tag="PWZ48_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDL2261075.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34054)
                     /locus_tag="PWZ48_001408"
     CDS             complement(33386..34054)
                     /locus_tag="PWZ48_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2261076.1"
                     /translation="MKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSGSGKSTF
                     LTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLTVKEQFE
                     LVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNEPSLILA
                     DEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDGYLTEEE
                     KKKE"
BASE COUNT        10479 a         6187 c         6500 g        10905 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga attaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc a
//
LOCUS       DANKEF010000026        33263 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000026 DANKEF010000000
VERSION     DANKEF010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.026"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(41..229)
                     /locus_tag="PWZ48_001409"
     CDS             complement(41..229)
                     /locus_tag="PWZ48_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDL2261077.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="PWZ48_001410"
     CDS             499..861
                     /locus_tag="PWZ48_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2261078.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="PWZ48_001411"
     CDS             913..2595
                     /locus_tag="PWZ48_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HDL2261079.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="PWZ48_001412"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="PWZ48_001412"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDL2261080.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="PWZ48_001413"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="PWZ48_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDL2261081.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="PWZ48_001414"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="PWZ48_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDL2261082.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="PWZ48_001415"
     CDS             6430..7434
                     /locus_tag="PWZ48_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2261083.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="PWZ48_001416"
     CDS             7435..8379
                     /locus_tag="PWZ48_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2261084.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /locus_tag="PWZ48_001417"
     CDS             8379..9341
                     /locus_tag="PWZ48_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261085.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="PWZ48_001418"
     CDS             9368..10282
                     /locus_tag="PWZ48_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261086.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="PWZ48_001419"
     CDS             10308..12089
                     /locus_tag="PWZ48_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2261087.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /locus_tag="PWZ48_001420"
     CDS             12437..13123
                     /locus_tag="PWZ48_001420"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDL2261088.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="PWZ48_001421"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="PWZ48_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HDL2261089.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="PWZ48_001422"
     CDS             16733..17542
                     /locus_tag="PWZ48_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2261090.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="PWZ48_001423"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="PWZ48_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDL2261091.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="PWZ48_001424"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="PWZ48_001424"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HDL2261092.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="PWZ48_001425"
     CDS             complement(21094..21546)
                     /locus_tag="PWZ48_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HDL2261093.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="PWZ48_001426"
     CDS             21696..22646
                     /locus_tag="PWZ48_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2261094.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="PWZ48_001427"
     CDS             22639..23598
                     /locus_tag="PWZ48_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HDL2261095.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="PWZ48_001428"
     CDS             23595..24350
                     /locus_tag="PWZ48_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2261096.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="PWZ48_001429"
     CDS             24373..25326
                     /locus_tag="PWZ48_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2261097.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="PWZ48_001430"
     CDS             complement(25970..26266)
                     /locus_tag="PWZ48_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HDL2261098.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="PWZ48_001431"
     CDS             26621..26977
                     /locus_tag="PWZ48_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2261099.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="PWZ48_001432"
     CDS             26987..27886
                     /locus_tag="PWZ48_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2261100.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGCYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28403..29134
                     /locus_tag="PWZ48_001433"
     CDS             28403..29134
                     /locus_tag="PWZ48_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HDL2261101.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="PWZ48_001434"
     CDS             29279..31306
                     /locus_tag="PWZ48_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2261102.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="PWZ48_001435"
     CDS             31414..32511
                     /locus_tag="PWZ48_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HDL2261103.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32818..>33263
                     /locus_tag="PWZ48_001436"
     CDS             32818..>33263
                     /locus_tag="PWZ48_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261104.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11138 a         5762 c         7122 g         9241 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtacttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggga accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagcatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctgttacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa
    32581 tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt
    32641 gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg
    32701 tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat
    32761 ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg
    32821 aattatgagt tttttttaag caaagaagat cacagaagtt attcattact taaatattta
    32881 gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg
    32941 acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta
    33001 gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa
    33061 tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta
    33121 ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg
    33181 agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa
    33241 attttttttg aaaagagaaa gtt
//
LOCUS       DANKEF010000027        32908 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000027 DANKEF010000000
VERSION     DANKEF010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32908
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.027"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            131..874
                     /locus_tag="PWZ48_001437"
     CDS             131..874
                     /locus_tag="PWZ48_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261105.1"
                     /translation="MKEISANEAHIPQKNQVGIYKVLHGEGIYKIQLLTGYNNGANGK
                     WLKATSIRELRSEVEKIRLGLTSEEIAKLKGNNSFVIEKEKEKHLLYRELKIEEYYEI
                     DLPEKLRIIDQRSEEKWKTEIYPSLNDYKTSGKPMYNYTGFLTYTQYKYAFRKLKGIK
                     QAEIALYEMKKVDRLKLIQDLVQNTWNQAIVELEKENGKILTNKYLIVNEIEELIGLF
                     SCEKGEVFLKSSPTYSILDSPYQFIQARR"
     gene            880..1244
                     /locus_tag="PWZ48_001438"
                     /pseudo
     CDS             880..1244
                     /locus_tag="PWZ48_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1260..1790
                     /locus_tag="PWZ48_001439"
     CDS             1260..1790
                     /locus_tag="PWZ48_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261106.1"
                     /translation="MSQNISAVKQLIEEDLETTVQRLAERIHFANYQTISNWSSRNRN
                     PEEIPMKFFYRIGKEYGVDPIKIFEKTMIYYYHGTPNEVLEHLLEEAKEKSSILELSQ
                     NDKVIQVKCVDPTGKIIVGFDIERKSKIDFITQLKKINNLDILTLRPDHEQLFMSFKK
                     QLKEIQKRYNKVFPNA"
     gene            1952..2260
                     /locus_tag="PWZ48_001440"
     CDS             1952..2260
                     /locus_tag="PWZ48_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261107.1"
                     /translation="MWNNRLKTGLLLIVISCAMMIGMRIQREQSYFEVSANNVIEKCY
                     YGQHYWSEEVRENIDREYVQRIVWDAYSIKDYPKSLTSRLFYSEKDNQKLSDLMMKKV
                     "
     gene            2256..2675
                     /locus_tag="PWZ48_001441"
                     /pseudo
     CDS             2256..2675
                     /locus_tag="PWZ48_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352869.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2812..3612
                     /locus_tag="PWZ48_001442"
     CDS             2812..3612
                     /locus_tag="PWZ48_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261108.1"
                     /translation="MSEDLRELKLRKKQYDQYFSGLLNKHIEEGLDHDFGKDEYCLYC
                     ERYNEKMSEYLQLKKEIQKLEKKVHAWKYKDSMSGYVESQNKPRKKKQAYVLKMVNGE
                     ELIVLAYSEEEVRELVETPVRKKVETINEIDDSQYNSIYIETSHGGYKKLATFLRSSK
                     VGIISKKDISAVTDSFCTLSVPKAYFQYDSKRFERLEKFCRPYTVLKTKKFNKTKRVS
                     IQLPTGQISLGEKDYLIKVGKELIIKVPEHLFKENFEIRETLVWKEEK"
     gene            3649..3831
                     /locus_tag="PWZ48_001443"
     CDS             3649..3831
                     /locus_tag="PWZ48_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261109.1"
                     /translation="MAVNKILQVLIIDEFGGGEWFDVQSIDFEEEVVVIENIYGEVYS
                     YSLEGENIEDLRLIGE"
     gene            3942..4259
                     /locus_tag="PWZ48_001444"
     CDS             3942..4259
                     /locus_tag="PWZ48_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261110.1"
                     /translation="MKREEYKQRLNELLEEDETLTHGSPDEILYMIDNMVIFGGYELG
                     NRSVDHNILEFDDVSWEEILDWGILAVPETKTYISDTMVPFFEELDYKRLPKNENHIL
                     GGN"
     gene            4262..4494
                     /locus_tag="PWZ48_001445"
                     /pseudo
     CDS             4262..4494
                     /locus_tag="PWZ48_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321015.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4609..5088)
                     /locus_tag="PWZ48_001446"
     CDS             complement(4609..5088)
                     /locus_tag="PWZ48_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261111.1"
                     /translation="MTDKTSDLPKLAPKTSRVKSKKDGKTKNAVKKKSVKETSSLLLD
                     IPMSSFDDNTDETSVKIATHVGMNNQNDSFYNLLNSLRELEDKKNQVIASKAPDLTTD
                     LDAEIIQIKQDERSVKSLLKTYFDGFDKELAPYSQAILTKPIQAPHISISLQKKSDD"
     gene            5208..6545
                     /locus_tag="PWZ48_001447"
     CDS             5208..6545
                     /locus_tag="PWZ48_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HDL2261112.1"
                     /translation="MLMVQFTGIAMSVITINPQVIHADGIATYDAQFNCTTEGSGSGT
                     DSDGNDTGSGAVTGDVAKNIEDIYKYMNGEYGLSGASIAGILGNWKQESGIDPRAVEG
                     YYGDGQREVDFIKQFTDGLMGIGYGQWTAGRHTALVDWSKKNNGGSWWTTEAQLDFMF
                     KGDGGFVGILKDYALNASDDVVENAVTFHKNWEISADSESAVIAVRGENAKEIWKYMK
                     SKGMDGAKDESKINKISGGSSTGDGSNNSSSSASGTVEDACATTDDANSGSGSIGKEG
                     KINGKKGQVIANMTYEEALKKYGKAITLPKFKEPDWSTSPFNGIYQGQCTELTWAYMS
                     QLYDGNQPTTGNGGYVWESYKSAGAKITNKPIVGYGFSASDGWLWAVGDAGHTGVVIA
                     VFEDGSFLCANFNVPPTQAPTRGVSVTLIDGVDGSDNVHFFSGVGKAKFEIEK"
     gene            6568..7095
                     /locus_tag="PWZ48_001448"
     CDS             6568..7095
                     /locus_tag="PWZ48_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261113.1"
                     /translation="MNSKVKDTIIISLLSGMLLALLVGILFFNKQNQDIEAEKHSVHI
                     EDKLTFFKEVKPNEVDGLRKDLENDIQEMLNGSVQNKFADQSGEEFLRKLFIVTGKPP
                     ITDKSSEKEKIEYYKPFTFKLTNFGIKTSSSGLYEALTDFEVQYNGKKVYDTTLTITL
                     NNDKKVTGGAFYAKS"
     gene            7082..7645
                     /locus_tag="PWZ48_001449"
     CDS             7082..7645
                     /locus_tag="PWZ48_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261114.1"
                     /translation="MQNHEITTAKKAIGILSIIVILCCVVLFQKKNAYDNNVKELEDL
                     KSKQIEVVKNNQKVKAMQEAQYKELGNAEIKEQSRKFFNLFYNWNSWEKYTKNMKEIQ
                     KEFPQIVKSSPVDISGTMVGTGNSPISSYTADYYTTNQKGKMVELITQTRNTPTTATT
                     TIWRGVTSIGENQLFYLEDLDSYVRAE"
     gene            8067..8804
                     /locus_tag="PWZ48_001450"
     CDS             8067..8804
                     /locus_tag="PWZ48_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261115.1"
                     /translation="MADHKLEKLLEETWNPKEFSEFFMENFETDLAVIVKDALREQGY
                     PETANYININFTLYTENKGTWDFWATLANKELSDKSDTGIRNFFESNRDDYMYANNQN
                     KLNFRVEFDETPEEFIERQPPKENVAKVLEDRWNSDEIVSTISELGGQYEPLVEAVRE
                     ELRLNKFPDVQNIDVSQIEINVKITNKLDYGSWADIALEKYIYSTLKEFIENRMDIMY
                     LQHPQYLNFGVEIATPLEEWKMEQGLD"
     gene            complement(8821..9237)
                     /locus_tag="PWZ48_001451"
     CDS             complement(8821..9237)
                     /locus_tag="PWZ48_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261116.1"
                     /translation="MNKKRKLSVLITLVSILTILSVILSLKIFNQINTSSNAKDGIVV
                     ENTSNSKETGTTTKKDKSAEITTKNYLIKTVYFDERKTAILKDEEGSELKLPITVSDK
                     FNGTFSLQTAEGSPDNLKVGQTIILGKNDELYSFVK"
     gene            9630..10877
                     /locus_tag="PWZ48_001452"
     CDS             9630..10877
                     /locus_tag="PWZ48_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2261117.1"
                     /translation="MNMKRNSLGMVLLASAVLAGLSVPVTSHADEWTANTPDQIQITT
                     GQSSYAFKKGDTIWSTVEKLKSLGYKTTVAEFAELNNVDLAKGEQRTIPIGRTFNISK
                     DTLTVKEADGEVVSQTVQKDKPDPIENTEQENKKSETTKESEPTANTSDKTETSASSV
                     DGASSSNTNNSTSEKEASQGTSESSTTEADTGTSTNNSQSTSNDANESTSTSSSDQSQ
                     TGSSDETDSSTSPSKPEQPTNVVLGVPFISQGNTMLCEGTSLLEALHYKGVATNQDLM
                     TFVKSMPLSPDNNPNNGFSGEWRHNVDGTYQGMNPAPVVSWGVANGGNVVDISGSSVQ
                     GLKDEIVNGNPVVAWVTYAFEPAQHKPMFWGDAIWNRHVVAVDGFKDGFYHVVDPVFG
                     ASWINAGSFETAYNVSQLAVSVR"
     gene            11145..13535
                     /locus_tag="PWZ48_001453"
     CDS             11145..13535
                     /locus_tag="PWZ48_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-Gln thioester bond-forming surface protein"
                     /protein_id="HDL2261118.1"
                     /translation="MKHEKTIILKSKIIYCFATLVMMLSTLIMPFSAIRAEADVTKPT
                     SAKFIETGVKTDGYIRVNMPNHPNEWMISSQFKDSHGNIGYCMDSELPSPTGSGAGSL
                     KYKGAGSDEFYRMFKGGFPSKTAKELGAGNDTEAWYATQLVSWVLAGNFKVSQIVWSH
                     PNHTAAETARVKKAFEKIYDYAKNGKDTPNTEFSITASKTADEGKYHTFTYKTASNKT
                     GNAKLTFTSAKPAGMKIYDADGKEITNNTVKLNSSFTIKVPVTTPSGTLSFKGTANVS
                     TTNPFTFDGRGVYQDAVVMITTSETKDSKSLSAKWTRAVGQVTVKKVDQDGNALKGAE
                     FTLTDNNNAKSVKTTGDDGLATFDLQQGLTYSLEETKVPEGYQGSFKKDGITVNTDGQ
                     EFSYTATNTIKKGKVKVKKVDQDGKPLANAEFTLTDDSSQTQAEKTDENGLASFDIVA
                     NKTYSLEETGVPTGYTGVFKQEGITVKDDGQTFEYTATNTLKKGLVKVHKVDQDGKPL
                     ANAEFTLTDEKGATQVEKTDENGLASFNIVANKTYSLEETGVPVGYTGAFKQEGITVK
                     DDGQTFEYTATNNLKKGVVKVHKVDQDGKPLADAEFTLTDEKGVKEVKKTDATGLATF
                     NIIANKTYNLEETKVPTGYTGAFKQEGITVKDDGQVFEYTAKNTLKKGVIKVHKVDDQ
                     GKALANAEFTLTDDKGVKVVQKTDRNGLATFNIVANKTYKIEETQNPNGYTGKWSKEG
                     ITVADDGQTFEYTVINHLVPETVRTGGIGTSAFLLVFLIAGLSMVLLYYREQHKAK"
     gene            13584..15419
                     /locus_tag="PWZ48_001454"
     CDS             13584..15419
                     /locus_tag="PWZ48_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2261119.1"
                     /translation="MKSKKIFSNLMLFGTLLGVGLGASSISSSLGLGGQVAYAQQNYN
                     YQGTGTIHIHKVTNTNGDGNPVSDIDNKGKEIALPNGVKPLEGVEFSYVKLTKDEAKS
                     LGWTDSDTTKGQHIDDAKARLLLNKKYSEVSRMKTDSQGNIDIPIQVNTDDLTDNLYL
                     IVETGTVKGVVEEAYPMIVNVPELNGSDDQYFNYDVHVYPKNYFQLNSAELDKKDEDG
                     KALAGTVFELRKDDGSDQVVGTYKTDSTGKLTVDGLELGDYYFLETDLGTDANRTTYL
                     LDGKKFKFSVKRGETAKVVLDVTNYKRPVITKVIDKETVDTGEDVQYTLKPSIPGNIG
                     NYEKYVVKDVLGAELTYDSSEVIEGMTLDTDYSIERDGQTTNYVFTESGMDKLQKQFD
                     AGDRFFTILMNAHTNSKAKMDTPLKNDVSTIYNNGYEPEDETPVDHAYTIIGDHQFIK
                     TDGDTSKALSGAKFVVKNKIDPNKVTYLKQDPTTKAVTWIADKSKATEFVSGSDGKFQ
                     IEGLQYTYDVDFDKQGNPTITDKKINDYALEEVVAPSGYTPTRNDVPFVVSNDDASSE
                     VTDIKNLKTPETVRTGGIGTVVFLVVAGVAGTGLYVVYKREQHKA"
     gene            15488..16351
                     /locus_tag="PWZ48_001455"
     CDS             15488..16351
                     /locus_tag="PWZ48_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2261120.1"
                     /translation="MSMKKKNIKLIILKIGMLLCLGVGLYPVITQAYTYYQTQQTLNS
                     YKEKYDEASEKENEEVFKRYSEVNRKRKDTEQTSVTIPEDNNNIPSNGNLQKDKLGEL
                     VGEILIPKINVDLPIYDGVNDFQLSAGAGILTGTDYPTGGIGNHSVISAHSGIPNKKY
                     FTDLELLKLGDKFFIDISGKKLAYQVDRIKTILPTEFEDLLPVAGKDYVTLMTCTPVG
                     DNSHRLLVRGVSIPYQKSIVEKEKKKEDLKVKAQMNWYPIVVVGVLLIIAVIVIIKKI
                     VAKRRGMKNEE"
     gene            16341..17450
                     /locus_tag="PWZ48_001456"
     CDS             16341..17450
                     /locus_tag="PWZ48_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2261121.1"
                     /translation="MKNKILLFLTLICTLVLTIYPANQLYHQFKTKENVAEIQEKIEK
                     KQTPSKEEVAKKVEQIKSNSGNQGLDNTQKTDKGVDNTQETDGGSFDIREAEGKVNPY
                     TGDVFSKNQTKDFEPSTYSGAVGYVYIPKINVLELLFLGASQEHLYRGVAQMSATSLP
                     LSGKGHQTVIAGHRGGYTSPQFLYVDQLVAGDHIYIDYLGEKAVYTVFRTDVIRNYGQ
                     NSTLTDIPDLDMLTLYTCHPYPQNYDRLLVRSLRDEKAKYITPQVTVGNTAYADAIDS
                     ESVSDKTETVTSSNDLFEDVDRANKELDGNNDLNIPEQGSVEMQDRTFAEKASSIFTG
                     ITNKHQKMLLVIWTVIGALASLKVLILLIKEFRKA"
     gene            17789..18589
                     /locus_tag="PWZ48_001457"
     CDS             17789..18589
                     /locus_tag="PWZ48_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261122.1"
                     /translation="MVGISKTALDSLAKLFSSRFDGAEFVEYLSQNESGKTLIETMKK
                     DYLNENDFPTWTNIEDATWDVVCTIYSPWVTIADFVLDKIGGDTYPDAKSLATSDFKT
                     FYGYLIGLIGNGIDLKVTPKQTPEEIEKEKGEVQMAYANDREKVKLLEGKLSEEELGK
                     MVQEINEDSNYFPELKVYQNDEDFFNEMFSNSPWEAVSKTNLGEWNFQDDLVAFSNDG
                     YLRSYSESEYRAELKTYETDILMSYLNLLESGQGELRDKVEELLKEEL"
     gene            18677..19483
                     /locus_tag="PWZ48_001458"
     CDS             18677..19483
                     /locus_tag="PWZ48_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261123.1"
                     /translation="MRGDQTMSATTDMALEAVIKDNTGNVENVEDFSDIAINLSAWIS
                     AVGEENIEFEIKNMDDLKAKFPFIASKLSQVAEDKSWNELVSFSEKIYDITQEDECVS
                     WEMNNQYKKIDAFVKSRIEGTAFEFEGIDTADNWLNKESFIKSIADDLEKQWSKEDYA
                     EYLKTERDTEVKEKLTEVCLDEHIDPNIVKQEDYEVVIDKVPTDFEEVVKYLVEEALS
                     QYSSDEEVDEETVRTRVWQLVFDDKDYKYLSDQNSVNWSVRLLIDQNDIE"
     gene            19715..20584
                     /locus_tag="PWZ48_001459"
     CDS             19715..20584
                     /locus_tag="PWZ48_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HDL2261124.1"
                     /translation="MKKKKFSRSLLGILVLVAVGLSGKGYFQNNSTQEHDFQIKSSVQ
                     IGNKADSESKEFLGIRYDPNKYPNNIVNINDGKYTLTKEDHEALSEKGSQTFWVNYAD
                     LDQLGRAGDVEALITYDAVNSHSSKNVERPSFKSSVHIAGEYKDGVFDQERDTWVGKD
                     SNNEITQLEGYKGYLYNKSHSLAWSLGGDMEVHNLTLGTRAQNVGKNDQNGGMAYSET
                     IVRDAIYKNKNEKVLYKVDPIYKGNELVPRGSHVEAFSVNDNGKTVNLNVYVFNNQKG
                     ININYQNGTWVRD"
     gene            20604..20951
                     /locus_tag="PWZ48_001460"
     CDS             20604..20951
                     /locus_tag="PWZ48_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261125.1"
                     /translation="METISINGLDKSVANRIKKGNGYYLLKKGKYSVARELETMSIVY
                     VAKNHFDVEQAIFDPDSEIGKCVELSEKTVSTKGAMVIEREMLDEIRVIIPKDTQMCE
                     DSISDDEPKSERK"
     gene            20964..22226
                     /locus_tag="PWZ48_001461"
     CDS             20964..22226
                     /locus_tag="PWZ48_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261126.1"
                     /translation="MSEKSIELKEYKRSDGEKYYPVASFSKSVYLVGRALVVGAIGIY
                     ILHWVTKVVQLLVDTLVSILVSLNDVNGTDDLGLKEMFKELLNAFFPNYLSYSYNVQS
                     FFAKLHLVDISILVLEAGTVFFVGYLVTLMFRHHYGEQRPFFDDMESRRLKREALEAM
                     DVKYDPNKIIGDETNNKKRKRNKTQLESDVLSTLKNIKISVHTRRDLSVNEDIREYIV
                     KIPQPKGKKVRETVLRQIQDLGSILTRATSGRAKFGEKEDEVSGDYFIFRASVNIERE
                     RKERVSTKNKETERATSSIAVSNSQFSFPINLFADNSKNIQNQTEKARKYAETQKQSL
                     ELYLSSADVPASLTDMHVGNTSIEYLFTLSRTRKIQLSSMKEEIETYLHISGVIVRIN
                     AGRIQITLPLGENDRIPIDVPSMINSVF"
     gene            22306..23682
                     /locus_tag="PWZ48_001462"
     CDS             22306..23682
                     /locus_tag="PWZ48_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HDL2261127.1"
                     /translation="MAMKLLEKVKKRAEKAESILLDKEPNIKLNGKFSVNMLNTKAKD
                     PTDAIFGIKPDGTIAHFCISEMPHMLVAGTTGSGKSVLLNEILVTVMCHSTPDELKIG
                     IVDPKRVEFGRYKKLPFMLADPITDMDEAYDFFEYLVILMHERYKLMEKAGLQNITLY
                     NEYAEKNGLDRFPYVILLVDEYSQLVGTHKEVEGLIVQLGQMARAAGIHVILATQSPR
                     STVVTGIIKANFPSRVCLMVASDLESRIVLDEGGGESLSPKGDMLIKLVDGSMVRAQG
                     AYISNKEIETIFNHLRNTMPEPEYVDYRAIVAETRGEGGEDEDFGGIGKAPVRKSAKR
                     NLNTPLANPSSKEPSIEKKSAFAIKLEKNKEEKANQGAKSLEKLQQEKLEKLEKALSS
                     STSETQSSKGIDNGQSITTKNKPKNSNSNLSVNVVITTDKSEKKQVNGTTSPIMQKMI
                     KAKKQVQK"
     gene            23679..24308
                     /locus_tag="PWZ48_001463"
     CDS             23679..24308
                     /locus_tag="PWZ48_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261128.1"
                     /translation="MIIISSFLRWLTPYKYTDEIVEKRAKKKVKKTMLTPKEMLDRGI
                     LGVKVLEVNQPFELDNARDRLLRAEVDVAYFYHDKSKHQIAIKFNPKSAWRYQARDDL
                     KRRKLKPMLYPEGKEAFDRTHIVPIGFHGTESDNRLLVGWNSSQNRVQINKFEKKVRK
                     FNDRKIILWFANIQKQPDGTAKWEAVIFDEDGKKVMSEVWHDKAKFVWQ"
     gene            24320..24790
                     /locus_tag="PWZ48_001464"
     CDS             24320..24790
                     /locus_tag="PWZ48_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261129.1"
                     /translation="MLTNLKLYREGFNEKVSIGESIEYLGKKYIIIRILEIKKLYFSK
                     YNNLAVRVLVQKVGNPSDYRSDYHNYKTESSVIKRYNQSKNDERIFKVGEIIISKYGI
                     AYEIVSIKKFYYEFVDLVVEYSVRLIVPWSKEEINEALKHERKSTFKVLEGEKK"
     gene            24787..25269
                     /locus_tag="PWZ48_001465"
     CDS             24787..25269
                     /locus_tag="PWZ48_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261130.1"
                     /translation="MKTDLTRQAEKCLWHETNRMGVFGCFEVTIGWDGSERVDYITYS
                     VDNTVRCYEIKVTLADLKSKAKQTFIGDYNYLVVTSDLWEKMQQDDKLMRKYYYQGVY
                     VFNEKYPSIISVKKARKQKVSLGTRSTVLESMVRSMNREVKKLYKVKPFWEIAEVEKG
                     "
     gene            25266..25928
                     /locus_tag="PWZ48_001466"
     CDS             25266..25928
                     /locus_tag="PWZ48_001466"
                     /inference="COORDINATES: protein motif:HMM:NF019467.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290663.1,RefSeq:WP_010747081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2261131.1"
                     /translation="MIQTFAKMSRSAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVELDEPKKSVDVPENVAELLDYYRNSSDVDLLALILTFHDWYYCQNEDTQHDKHD
                     KAVDWLIENPEKFMRAWLDGYDSKKKPLESNEETYLTAEQYDYARKTGDWESGKTFNH
                     RKDQPKISFSDIAAIIEGIYYGQADEEIFSIQDLLEFLNKNKDSFVELTDYEEDTANG
                     KL"
     gene            25915..26283
                     /locus_tag="PWZ48_001467"
     CDS             25915..26283
                     /locus_tag="PWZ48_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HDL2261132.1"
                     /translation="MENYDPNMRWGTHTLKVSFQRWDYKGFVTFRKAGNCKGLDVLAL
                     DEDYLYDHPLTDNPIGFGLLPEDDEGNEWFKMILTNDKGDQLFVEDTWSYLSEYIVSI
                     KIIDFVADKEKEIGEGKSNY"
     gene            26364..26816
                     /locus_tag="PWZ48_001468"
     CDS             26364..26816
                     /locus_tag="PWZ48_001468"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261133.1"
                     /translation="MIVSAVTIYWLGNQQNSLIVDLGELKVGLRMTRWLLLAQTVGLA
                     ILTWICVEKITSNNNQEIVESTNLRIRITEQAIEKQKDKEKKEIMSIYLSSGASFFHE
                     IDSMKLSSQEIIDRWKNMPLGGELIVEDSDGWSAYKKDEIVAITILFE"
     gene            27468..27521
                     /locus_tag="PWZ48_001469"
                     /pseudo
     CDS             27468..27521
                     /locus_tag="PWZ48_001469"
                     /inference="COORDINATES: protein motif:HMM:NF019230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27525..28124
                     /locus_tag="PWZ48_001470"
     CDS             27525..28124
                     /locus_tag="PWZ48_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDL2261134.1"
                     /translation="MREKNKNSSRYLVATVVFVCVLIFLFDFLQIGKSIIGNTLSAKE
                     MVALGGLYEGHLFPNIFISIVSHINLYHLISNLLFLVVLEEALNDQYEWLTLLIVLII
                     SGLLGSLVPFLLQSNIVSMGLSGGIFGWIALFAIAYIRFDSKEHLLLSVFILLIIGIL
                     LVSTFESGETNVLSHVIGFVCGMILGFILKNISLIKQKN"
     gene            28140..28616
                     /locus_tag="PWZ48_001471"
     CDS             28140..28616
                     /locus_tag="PWZ48_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261135.1"
                     /translation="MPKKKEKKIPVFLSVSEMLSLQILLRKKIETIDEEVRNKRAMYE
                     QREALRERIHYFDDIHTKIYEARTNNEDESKLGLAQQRIFEWLNTNTEQEPLRAISNL
                     FEFGTKHWFNAVEKDADLQEAFDTLSKDSKSMRDLLITYLNEHEFEQTKTKKKNKE"
     gene            28633..29535
                     /locus_tag="PWZ48_001472"
     CDS             28633..29535
                     /locus_tag="PWZ48_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261136.1"
                     /translation="MLLTLLGYLCLILAFFGAIAIIIGQFQTNNPIMRNIGIGIVIVT
                     FIVFNATKIFGSNDKQFGISGVNNSYAPDSLGRVVIDGTFENVTSYKNVSLTVKEDGK
                     KASDDVLATIKPSQSQFEVFYTVKENSPVHKLTLVFNVDGEEYYKDVKINKTSTKKII
                     ESAKKEVATEESTTESSSTVEAELPDKIDFDPEKVKSFESVSGSMADRFNTEVFKAIK
                     TVKLDTEKQMINVTLFNSVESMDQDKKVTVARELGKRVIGLYRGTAKPDSDGADLPVH
                     VVYEDGKVFGDSTANEALTIKLAK"
     gene            29608..29799
                     /locus_tag="PWZ48_001473"
     CDS             29608..29799
                     /locus_tag="PWZ48_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261137.1"
                     /translation="MVVNKEKVRKYVTLKIASVKYLEKRAKEEERARGKKVTVSELLE
                     ELIENDQTIRRAFTNRSIR"
     gene            29963..30550
                     /locus_tag="PWZ48_001474"
     CDS             29963..30550
                     /locus_tag="PWZ48_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261138.1"
                     /translation="MENNNDIRHFSIGERVTTVSELSKDYRDKEGTIISLNKNRRGVW
                     QYVVDLGDGVTVECKDSQLIRNNGSHSIEKAILVYPKVSSKIMNDWLLGKFGEYNKKE
                     IAEVIGKSYPTVLGYLKKITSKEAKIEDVLTVRELQSLIEYRNKQITNPEYLRKEDGL
                     LKQLEQVNKIRKMQKVAPLSLDTFKKIYLKEIKKD"
     gene            30550..31449
                     /locus_tag="PWZ48_001475"
     CDS             30550..31449
                     /locus_tag="PWZ48_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDL2261139.1"
                     /translation="MWTVTLPFQPVSAPRPSARRNAADETNDVAEKRISTYYDKKYLD
                     YLKKIKSFIEDQDLLDDEFYSIVNDPMGAIMEVDFYYQIPKSYKKVYNIMKTTAPDLD
                     NLVKSAMDGIFNISAIRDSHITGLIAFKYNVANEGKTVVRIKRYSEFEWDTSEGLNGD
                     IWEATFDFKPVATPRPKSKFRGNGIITYYPKEYNDYLENIKNTLKEKDLVNDQLKKVM
                     NAPMGVIAQIDYFYQVRKDKKKLDSLMRTSQPDIDNLVKGTLDGIFNKEIGIKDSRVV
                     GLIAFKYNTFEKSKTNVRLQEMG"
     gene            31449..32363
                     /locus_tag="PWZ48_001476"
     CDS             31449..32363
                     /locus_tag="PWZ48_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261140.1"
                     /translation="MNNNEKILHVLDSFEIIQEELKKYRDVLEQRYDFVNQQKSNHMD
                     FILNMNDLKKKLVERKEQEKLIKAYFELGEKEVKNAMELNEERRVLDQLLEQLLVMFQ
                     KGRIDEDLIEEGLRKYPSNSGIGIVLKAIDEEEIEAFIPPEDFESAMEYIKYYSQGIT
                     AFREFDPEDVIHDLNNLKEWCESYGVDDSGLDYLISIMEIEEEMPDKPDPTDILELIH
                     EARNPIAYISRGYTVLEYYKPYISAMNHLRRVLREKREYRSVLNATNRLEKAVSELDA
                     YYREHYLQAGGMPRNTKANISRYIKKAE"
     gene            32415..32630
                     /locus_tag="PWZ48_001477"
     CDS             32415..32630
                     /locus_tag="PWZ48_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261141.1"
                     /translation="MKRDVFELYVLERLEDGMMAQEIIDEIDDMISRFGQLELNDGNK
                     NSVVLKQFWLNNFSYKDFIKEKANTLY"
BASE COUNT        12443 a         4451 c         6662 g         9352 t
ORIGIN      
        1 atctttaggg cgtaagccct atttttttaa aattaagtga taacaaataa atgatttctg
       61 aaacaaacgt tcttactcaa ctactcgaaa cacacaagct aattaactag attataaagg
      121 agaatgcgta atgaaagaaa tcagtgcaaa tgaagctcat attccgcaaa agaatcaggt
      181 tggtatatat aaggtgcttc atggtgaagg aatttataaa atacagttac ttaccggtta
      241 caataatggt gcaaatggga agtggttaaa ggcaacaagt attcgtgaac ttagaagtga
      301 agtagaaaaa attaggcttg gtctcacaag tgaagaaata gctaaactga agggaaataa
      361 ttcttttgta attgaaaaag aaaaggaaaa acatctactt tatcgtgagc tgaaaattga
      421 agaatactat gaaattgatt tacctgaaaa attaagaatt attgatcaac gttcagaaga
      481 aaaatggaaa actgaaatat atccaagctt aaatgactac aagacatcag gaaagcccat
      541 gtataactac acaggctttt taacctacac acaatacaaa tatgcctttc gcaaattaaa
      601 aggcattaaa caagctgaaa ttgccctgta tgagatgaaa aaagtggata gattgaaatt
      661 gattcaagat ttagtgcaaa atacatggaa tcaagcgatt gtcgagctag aaaaagaaaa
      721 tgggaaaatt ttaactaata aatatctgat cgtaaatgaa attgaagaat taattggttt
      781 attttcatgt gaaaaaggtg aagtcttctt gaagtcatca ccaacctatt caattttgga
      841 cagtccgtat cagtttatac aggcaaggag atgagttaga tgtctagctt gaaagatgac
      901 tatatccaag caaaacaaga gtatgaaaaa atacagatgt tgaaatctga gaaagtcgaa
      961 ttatttttaa cacaaaaatt agcagaggat catattgcta acttagatgc taaaacttta
     1021 gaggaatatg atcggcagtt taatcagata catcacaata tcattttgct tgttgagcag
     1081 gcaaagcgaa ggtttgaaaa agcggaatat gatctattag atcaaggaat tgtatgcttg
     1141 gataagggat tgcgagatat tatggtgcag agttaaaaaa gcaaaatgta gatagaaatg
     1201 cactaaaaca tgaaatctta gaattgataa tgcaacgaat ttgaagggat agaaagcaaa
     1261 tgtcacaaaa cattagtgct gtaaaacaat taatagaaga agatttagag accacagttc
     1321 agagattagc ggaacgaatc cattttgcaa attatcaaac catctctaac tggtcaagca
     1381 ggaacagaaa tccggaagaa attccaatga aattttttta tcgaatcgga aaagaatatg
     1441 gtgttgatcc gataaaaatt ttcgagaaga ccatgattta ttactatcat ggaacaccta
     1501 acgaagtgtt agaacatctt ttggaagagg caaaagaaaa atcatcaata ttagagctat
     1561 cgcaaaatga caaagttatt caagtgaaat gcgttgatcc aacaggaaag attattgtcg
     1621 gttttgatat tgaaagaaaa tccaaaatag attttattac acaattaaaa aagataaata
     1681 atttagatat tctaacgctt agaccagatc atgaacagtt gtttatgagt tttaaaaaac
     1741 aattgaaaga aatacaaaaa agatataaca aggtgtttcc taatgcgtga aaagctcaaa
     1801 gaaatcagag ttttttggat aattaacaaa gaatgtatca tcttatatac tcttggattt
     1861 cttagcatga tcttgttttg gtggttatat ttaaaacagt aattggagag ggtcaagagg
     1921 atttttatca agataaggat gtggaaactg catgtggaat aatagattaa aaacaggatt
     1981 gttattaata gttatttcct gcgctatgat gattggtatg aggatacaaa gggaacaatc
     2041 ctattttgaa gtttctgcta acaacgtaat agaaaaatgc tattacggtc aacattattg
     2101 gagtgaggaa gtcagagaaa atatagatcg tgaatatgtt caaagaattg tttgggatgc
     2161 atattctatc aaagattatc ctaaaagttt aacgagtaga ttattttaca gtgaaaaaga
     2221 taatcaaaaa ctctcagatt tgatgatgaa aaaagtatga ctattattac gaaagagaaa
     2281 cagagtgcta tattcgaggg gaaaaagcct acaacaaaag tgactattgg ggtgacaagg
     2341 cgaagcgatt aactgcaaat tttagagtgt atgaatcaga gtttataaac ttgacctttc
     2401 ttaatagtgt ttttattgat tatgtaatta ctacgaaacg cattggaagt tattcaagta
     2461 cctacgcatc cttagttcca atgttaaact cgatgagccg tttcttaaaa gaaagagaaa
     2521 agaaagagct acagtatatc aaagaaatca tgccggattt tgaaatgcac gaatggcaaa
     2581 ttgctttatc agattggaaa ttggcgaata aggttcgtag aataacacct tttcaagcca
     2641 agcgcttttt gagagctatg aaatttgtgc aataaaaaaa gccaccagac aagttgatgg
     2701 agactaacac aattaccaac agttgtaatt gtgttagctt acaaattaat tatggtgttt
     2761 agtcaatccc aaattgtgaa atggcaccaa taattggaaa ggaaaaatga aatgtctgaa
     2821 gatttacgag agctgaagtt acgaaagaag cagtatgatc agtatttttc cggtttgttg
     2881 aataaacata ttgaagaagg acttgatcat gattttggaa aagatgaata ttgtttatat
     2941 tgcgaacgtt acaatgaaaa aatgtctgaa tatctacagc tgaaaaaaga gatacagaaa
     3001 ttagagaaaa aagtacatgc ttggaagtat aaagattcca tgtctggtta tgttgaatct
     3061 caaaataagc caagaaaaaa gaagcaagcg tatgttctaa agatggtaaa tggggaagag
     3121 ttaattgtat tagcgtacag tgaagaagag gttcgagaac tcgttgaaac acctgtcagg
     3181 aaaaaggttg aaacaataaa tgagatagac gacagccaat acaatagcat ctatattgaa
     3241 acaagtcatg gtggatataa gaaattagca actttcctta gaagttctaa agttgggata
     3301 atttctaaaa aagacataag tgctgttacc gatagttttt gtactttatc tgttcctaaa
     3361 gcttatttcc aatacgatag taaaagattt gaacgcttag agaaattctg tagaccgtat
     3421 actgtattga aaactaaaaa atttaataaa actaaaagag tttctataca gttaccgact
     3481 ggtcaaatct cattaggtga aaaagactat ctgataaaag taggaaaaga gttaattata
     3541 aaagtaccgg aacacttatt taaagaaaat tttgagatac gtgaaacctt ggtatggaaa
     3601 gaagaaaaat agtttggaag acatagaatg gtataagggg aaattgaaat ggcagtaaat
     3661 aaaatattgc aggtactaat catagacgaa tttggcggag gagaatggtt tgatgtgcag
     3721 tccatagact ttgaagaaga agtcgtagtt atagaaaata tttacggcga agtatattca
     3781 tattcgcttg aaggtgagaa tattgaggat ttacggttga ttggtgagta gaaaaaaaca
     3841 gaaatatcaa aatggagata aaaattaata gcatgtaaag atagtcatgc ttgggttaaa
     3901 aatgttaaaa ttcaaaaaaa tgagtcagaa gggataaaag tatgaaaaga gaggaataca
     3961 aacaaagatt aaacgaactg cttgaagagg acgaaacatt aactcatggt tcgcctgatg
     4021 aaattctata catgattgat aatatggtga tctttggtgg atatgaactt ggtaataggt
     4081 cagttgatca taatatttta gaatttgatg atgtatcatg ggaagaaatt cttgattggg
     4141 gaattttggc ggtacctgaa actaaaacct atattagtga cactatggtt cctttttttg
     4201 aggaattgga ttataaaaga cttcccaaaa atgaaaatca tatactggga ggaaactaac
     4261 aatgttaagt gacttattta ttttactgat ctatctaggt gtaagtgaaa ttattgcgta
     4321 ttttttgttt gtcattgcta attatggtat ttccagtgca atcgttcaaa tgtttagatt
     4381 accagtaagt gaactaaaaa aatgatgaga ttatcagcat atcttttaat gtttttgaat
     4441 gtggtcacgt taatctttgt agtaatcggt gtgactttcg atttaatgga atagtgggaa
     4501 atctataatg gaaaaaagag acacgaatga acatattttt tgaatcaata ttgttgatgg
     4561 tttttgcgat cagcgcttac cagaaatcta aaaaagagta atgattgttt aatcatcact
     4621 ctttttttgt agggaaattg aaatatgtgg agcttgtatt ggtttagtta ggattgcttg
     4681 gctataagga gctagttctt tatcaaaacc atcaaagtag gttttcagta gcgatttaac
     4741 tgatcgctca tcttgtttga tttggataat ttcagcatcc aaatcggttg tgagatcagg
     4801 agcttttgat gcgattacct gatttttttt atcttccaac tctcttaggc tgtttaacaa
     4861 gttgtaaaaa gaatcatttt gattgttcat tccaacgtga gttgcaattt taacagaagt
     4921 ttcgtccgta ttgtcatcaa aagaactcat tggaatatca agtagtaacg aggatgtttc
     4981 ttttactgac tttttcttta cagcattttt tgtttttcca tcttttttgc tctttactcg
     5041 agatgttttt ggagctagtt taggtaaatc acttgtttta tcagtcattt attagctttt
     5101 cctttctatt aatttatgtt attatattat cataataaca taaaaaagta cactttttgg
     5161 gggcgtagtt attgaaaaaa tctaagagaa agattgccac attgcttatg ttaatggtgc
     5221 aatttacagg gattgcaatg agtgtaatca caataaatcc gcaagtgata catgctgatg
     5281 ggatagctac ttatgatgca caatttaact gtactactga aggtagtggg tcaggaacag
     5341 atagcgatgg aaacgatact ggaagtggcg ctgtaacagg tgatgtagcc aaaaatattg
     5401 aagatattta caaatacatg aatggagaat atggcttatc aggtgctagt attgcaggta
     5461 ttttaggtaa ctggaaacaa gaatcaggta ttgatcctcg agcagtagaa gggtattacg
     5521 gagatggaca gagagaagtt gacttcatca aacaatttac tgatggtctg atgggtattg
     5581 gctatggtca atggacagct gggcgacata cagcattagt tgattggtct aagaaaaata
     5641 atggcggttc ttggtggact acagaagccc aattggactt tatgttcaaa ggtgatgggg
     5701 gattcgtagg aatattaaaa gattatgctt taaatgcttc tgatgatgtc gtagaaaatg
     5761 cagtaacttt tcataaaaac tgggaaattt cagctgattc cgaatcagct gtaatagctg
     5821 tacgtggtga aaatgcaaaa gaaatctgga agtacatgaa aagcaagggt atggacggtg
     5881 caaaagatga aagtaagatc aataaaatat ccggaggttc aagtaccgga gatggatcga
     5941 ataattcatc aagttcagca tcaggaacag tagaagatgc ctgtgcaact acggatgacg
     6001 ctaattctgg ttctggatca attgggaaag aaggaaaaat taacggtaaa aaaggtcaag
     6061 taatagcgaa tatgacttat gaagaagctt tgaaaaaata tgggaaagcg atcacgctac
     6121 caaaattcaa agaaccagat tggtcaactt caccttttaa cggaatatat caaggtcagt
     6181 gtacagagtt gacgtgggct tatatgagcc aattatacga tgggaatcaa ccgactactg
     6241 gaaatggtgg ttatgtatgg gaaagctaca aatcggcagg agcaaaaata acgaacaaac
     6301 caatcgtagg ttatgggttt agtgcttcgg atggatggtt atgggctgtt ggagatgcag
     6361 ggcatactgg tgtagttata gcggtgtttg aagatggatc atttctatgt gctaacttta
     6421 atgttccacc aacccaagct ccaacaaggg gagtttcggt aacgttgatt gatggagtgg
     6481 acggatcaga taatgtgcat ttctttagcg gtgtaggaaa agctaaattt gaaatcgaaa
     6541 aataaattgg aatgaaggac gatatagatg aatagtaaag tgaaagatac aattatcatt
     6601 tctttgcttt ctggtatgtt attagcactg cttgttggaa tcctattctt caataagcag
     6661 aatcaggaca tagaagctga aaaacacagt gtgcatattg aagataagct gacattcttt
     6721 aaagaggtta aaccaaatga ggtggatgga ttacggaaag atttagaaaa tgatattcaa
     6781 gaaatgttga atggatcagt tcaaaataag tttgcagatc aatcaggtga agaatttttg
     6841 agaaaactat ttattgtaac aggtaaacca ccaattactg ataagagtag tgagaaagaa
     6901 aaaatcgagt actataagcc ttttactttt aagctaacaa actttggaat taagacatct
     6961 agtagtgggt tgtatgaagc attgaccgat tttgaagttc aatacaatgg aaaaaaagtg
     7021 tatgatacca cattaaccat cacactaaat aatgataaga aagtgactgg gggtgctttt
     7081 tatgcaaaat catgaaatca ctactgctaa aaaggcgatc ggaatacttt ccataattgt
     7141 gatcctttgt tgtgtggtac tatttcaaaa gaaaaatgct tatgataata atgtcaaaga
     7201 attggaagac cttaaaagta aacaaatcga agtagtcaag aataaccaga aagtgaaagc
     7261 tatgcaggaa gctcaataca aagaattggg aaatgctgaa attaaggaac aaagtaggaa
     7321 attctttaat ttattttaca actggaattc atgggaaaaa tatacgaaga atatgaagga
     7381 aatacaaaaa gaatttcctc agatagtaaa gtctagtcct gttgatattt ctggcacgat
     7441 ggtaggcact ggtaatagtc caatatctag ctatactgct gattactata caacaaatca
     7501 aaaaggtaaa atggttgaat tgattacaca gacaagaaat actccaacaa cagcaacaac
     7561 aacaatttgg cgtggagtaa caagcatagg agaaaatcaa ttgttttatt tggaggattt
     7621 agatagctat gtaagagcag agtaaaataa aaaaagagct aacggtacca acgttagctc
     7681 tttgtgctga cgatgataac aaaacctacc gaaaaaggat tgtttcatca caaacacaaa
     7741 cagaaaagaa ttgataaaaa acccgaacct cagaattttt attaatcaat tcatttagat
     7801 tataataaat tgagctgaac attacaatac gtatatgttg taaaacgtaa aaaaagtcaa
     7861 aagtaaatac tttcttaaaa agtcaaataa aactattaaa gacaatatat atggtttatt
     7921 tgacataaga aaatggtcta gatgataatt gtaatataga attgtagact taaataaagc
     7981 ataaaatacc ttttatgata aggtatagct cttaatttta agcagtttta gatataagat
     8041 tgaaagtaaa gtgggggaga ataataatgg cagatcataa attagaaaag ttattagaag
     8101 aaacgtggaa tccaaaagaa tttagtgaat tttttatgga gaattttgaa actgacttag
     8161 cagtgattgt taaagatgct cttagagagc aagggtatcc tgaaactgca aactatataa
     8221 acattaattt tactttgtat actgaaaata aaggaacttg ggatttttgg gctactttag
     8281 caaataagga gttaagtgat aaatcagaca caggaattcg taactttttt gaatcaaaca
     8341 gagatgatta tatgtatgct aacaatcaaa ataaattgaa ctttagagta gagtttgatg
     8401 aaacacctga agaatttata gaaagacaac cgcctaaaga aaatgtagca aaggtattgg
     8461 aagatcgttg gaattcagat gaaatagttt caacaattag cgaactaggc ggacaatatg
     8521 aaccgctagt agaagcggtt agagaagagc taagactgaa taagtttcct gatgttcaaa
     8581 atattgatgt ttctcaaatt gaaataaacg tcaagatcac gaataaactt gattatggta
     8641 gttgggcaga tatagcatta gaaaaataca tctactcaac gttaaaagag tttatcgaaa
     8701 atagaatgga tatcatgtat ctacagcatc cacagtattt aaactttggt gttgaaatag
     8761 ctacacctct tgaagaatgg aaaatggagc aagggcttga ctagcctttg ctccattttt
     8821 ttattttaca aatgaataca gttcgtcgtt tttaccaaga atgattgttt gcccaacttt
     8881 gagattgtca ggtgatcctt cagcggtttg taaggagaac gttccattga acttatcgga
     8941 taccgttata ggtagcttta attctgatcc ttcctcatct ttgagaattg ctgtttttcg
     9001 ttcatcaaaa taaacagtct ttattaaata atttttcgta gtgatctcag cagatttgtc
     9061 tttttttgta gtagtaccag tttctttaga attagaggta ttttctacga ctattccatc
     9121 tttggcattt gagcttgtgt ttatttgatt gaatattttc agcgacaaga taacgctaag
     9181 aatagtcaat atagaaacaa gtgtaattaa tactgataat ttcctttttt tgttcatttt
     9241 ttcttaacat ccttttttgt tttgattagt tagatattcc ttatctaaca agccttttta
     9301 ggctattaaa tgaagataaa ccccaaaaaa atattgcaat tatgacagtg caatggtact
     9361 attattatac aataaatttt gcgaaagggg taggtctaaa tcgcaaacaa aaagaaattg
     9421 aacctagaca aatacaactt gtataccgca agggaagcaa gtttgatttt gggtaaaggt
     9481 agaaattatg ttacaaacat aatcaactat tatccaaatc gtttgcctaa aggttcgttc
     9541 aagaaagtgg gtgacaaaac ctatattata acggaagaag gaatagaagc attgcgatca
     9601 caaacagaaa ataaaaacaa aaagaaggaa tgaatatgaa gagaaattcg cttggtatgg
     9661 ttttattggc atcagctgta cttgcggggt tatcagtacc agtaacctca catgctgatg
     9721 aatggactgc aaacacaccg gatcaaatcc aaattacaac aggacaatca agttatgcgt
     9781 tcaaaaaagg agatactatt tggtcaactg tagagaagct taaaagctta ggttacaaga
     9841 ctactgttgc tgagtttgcc gaattgaaca atgttgattt ggcaaaagga gaacaacgga
     9901 ctatcccgat tggacgaact ttcaacatca gtaaagatac attaacagtt aaggaagcag
     9961 atggcgaagt agttagtcaa actgttcaaa aagataaacc tgatcctatt gaaaatacgg
    10021 agcaagagaa caaaaaatcc gagactacaa aagaatcgga accaacagca aacacttctg
    10081 ataagacaga aacaagtgct tcaagtgttg atggagcaag ttcatctaat acaaataatt
    10141 ctacaagtga gaaagaagca tcacagggaa caagtgaaag tagcactacg gaagctgata
    10201 ctggaacatc aacaaataac tcacaatcaa catcaaatga tgcaaatgaa tcaaccagta
    10261 catcttctag tgatcaatca caaacaggtt cgagtgatga aactgacagt tctacttcac
    10321 caagtaaacc cgaacaacca actaatgttg tgctaggcgt tccatttatt agtcaaggta
    10381 acacaatgct ttgcgaagga acatctttgc tagaagcgtt acattataaa ggtgttgcaa
    10441 caaatcaaga tttaatgact tttgttaaat caatgccact atctcctgac aataatccaa
    10501 ataatggttt ttctggtgaa tggagacaca atgtggatgg aacttatcaa ggtatgaatc
    10561 cggctcctgt agtttcatgg ggtgttgcta atggcggaaa tgttgtagat atttctggtt
    10621 catcggtaca aggattaaaa gatgagattg taaatggaaa tcctgtagta gcgtgggtaa
    10681 cttatgcctt tgaacctgct caacataaac caatgttttg gggcgatgct atttggaatc
    10741 gacatgtagt agccgttgat ggatttaaag acggttttta ccatgttgtc gatcctgtgt
    10801 ttggggcaag ttggattaat gcaggatcat ttgaaacagc atataacgta tctcaattag
    10861 cggtttcggt acgttaataa aagatatcgt ttatatacta agtagttata tagtctttat
    10921 ttgtccttgc ttaactactt agaaaacttg ttgctcatgg tgggtagaac taaaaggggt
    10981 gatgctatga atactagagc gttgaaacag cttgctttca acgaaaaaaa tgattaatag
    11041 gtgtggtgat tttaacttag gaatgggcgc gaaggtcatt accacaccga catatcgtgg
    11101 ctcattttct tcgcctaact ggtataaaga aggagtaacg agagatgaag catgaaaaaa
    11161 cgataatttt aaaatcaaaa ataatttatt gttttgcgac tttagtaatg atgttatcta
    11221 cgcttataat gcctttttca gcaataagag ctgaagcaga tgtcacaaaa ccgacaagtg
    11281 caaaatttat agaaactggc gtaaaaacag acggttatat tagggttaat atgccgaatc
    11341 atccaaatga atggatgatt tcaagtcaat ttaaagattc tcatggaaat attggatatt
    11401 gtatggattc agaattgcca tccccaacag gaagtggagc cggaagcttg aagtacaagg
    11461 gagctggcag tgatgagttc tatcgtatgt ttaaaggtgg ttttccatca aaaacagcaa
    11521 aggaactggg tgcaggaaat gatacagaag catggtatgc aacacaatta gtttcatggg
    11581 ttttagccgg aaactttaaa gtttcacaga ttgtatggtc acatccaaac cataccgccg
    11641 cagaaacagc tcgtgtaaaa aaagcatttg aaaaaattta tgactatgct aagaatggaa
    11701 aagatacgcc caacactgag ttttctatca cagcttctaa gacagctgac gaaggaaaat
    11761 atcacacttt cacatataaa acagcatcaa ataagactgg taatgcgaag ctcactttta
    11821 catcagcaaa accagctggt atgaagattt atgatgcaga tggtaaagaa attactaaca
    11881 acactgtgaa attaaattca agttttacta ttaaagtacc tgtgactaca ccaagtggta
    11941 ctttgtcatt taaaggtact gctaatgtta gcacaaccaa tccttttact tttgatggac
    12001 gaggtgtgta tcaagatgcc gtagttatga tcactacttc agaaacaaag gactctaaat
    12061 ctttgagtgc taaatggaca agagctgttg gtcaggtaac agttaagaaa gttgatcaag
    12121 atggaaatgc tttaaaagga gcggagttta ctctaactga caataacaat gctaaatctg
    12181 ttaagacaac cggagatgat gggttagcta cgtttgattt gcaacaaggc ttaacataca
    12241 gcttagaaga aacaaaagtt cctgaaggtt atcaaggttc atttaaaaag gatggtatta
    12301 ctgttaatac cgatggacaa gaatttagtt acactgctac gaacacaatt aaaaaaggta
    12361 aagtaaaagt taaaaaggtt gaccaagatg gaaaaccatt agctaatgct gaatttacct
    12421 tgacggatga ttcatcccaa acacaagcag aaaaaacaga tgaaaacgga cttgcaagct
    12481 tcgatattgt agcaaacaag acctacagcc ttgaagagac cggagtacca actggttata
    12541 caggcgtatt caagcaagaa ggaattaccg ttaaagatga tggacaaact tttgaatata
    12601 cagctacaaa cactttgaaa aaaggtttag taaaagttca taaagtcgat caagatggaa
    12661 aacctttggc aaatgcagag ttcactttaa ccgatgaaaa aggtgcaaca caagtagaaa
    12721 aaacagatga aaatggactt gcaagcttca atattgtagc aaacaagacg tatagcctag
    12781 aagaaaccgg agtaccagta ggttacacag gtgcatttaa gcaagagggt atcaccgtta
    12841 aagacgatgg gcaaactttt gaatatacag ctacaaataa tttgaaaaaa ggtgtagtaa
    12901 aagttcataa agtcgatcaa gatggaaaac ctttagcaga tgcagaattc accttaacgg
    12961 atgaaaaagg tgtaaaagaa gttaaaaaga cggatgcaac tggtctggct acgttcaaca
    13021 ttattgcgaa caaaacttac aacttagaag aaacaaaagt accaacaggt tacacaggcg
    13081 cattcaaaca agaaggaatt accgtcaaag atgatggaca agtctttgaa tatacagcta
    13141 aaaacacttt gaaaaaaggt gtgatcaaag ttcataaagt cgatgatcaa gggaaagcat
    13201 tagcaaacgc agaattcact ttgacggacg acaaaggtgt aaaagttgta caaaaaacgg
    13261 acagaaatgg cttggcaaca tttaatattg ttgccaacaa aacgtacaaa atcgaagaaa
    13321 cacaaaaccc taatggttat acagggaaat ggtctaaaga aggcatcacc gttgctgacg
    13381 atggacaaac ctttgaatat acagtaatta atcaccttgt acctgaaaca gtaagaacag
    13441 gcggaatcgg tacatcagcc ttccttcttg tatttctaat tgcaggattg agtatggtgc
    13501 tactgtatta tcgagaacag cacaaagcaa aataaaaaag tgttaatgga aaagaagtaa
    13561 atatgaaaaa agaaggctga cgtatgaaat caaaaaaaat tttttcaaat ttaatgctat
    13621 ttggtacttt gctaggagtg ggattgggcg caagctcaat ctcttctagc cttggactag
    13681 gggggcaagt agcatatgca caacaaaact ataattatca aggaactgga acgattcata
    13741 tccataaagt aacaaatact aatggggatg ggaaccctgt ctcagatatt gataacaaag
    13801 ggaaagaaat agctttacca aatggtgtaa aacctcttga aggcgttgag ttctcttatg
    13861 tgaaacttac taaagatgaa gctaaatcct tgggatggac ggatagtgat actacaaaag
    13921 gtcaacatat tgatgatgct aaagcacgat tattactaaa caaaaaatac agtgaagtaa
    13981 gtagaatgaa gactgattca caagggaata tagacatacc tatacaagta aatacagacg
    14041 atttaacaga taacttgtat ttgattgttg aaacaggaac agtcaaaggt gtagttgaag
    14101 aagcgtatcc tatgattgtt aacgtacctg aactaaacgg atcagatgat caatacttta
    14161 attatgatgt acatgtctat cctaaaaact atttccaatt aaactcagca gagcttgata
    14221 aaaaagacga agatggaaag gctcttgccg gaactgtttt tgaattgcga aaagatgatg
    14281 gatctgatca ggttgttgga acttataaaa cagacagcac aggtaaatta actgtggatg
    14341 gtttggaatt aggtgactat tatttcctag aaaccgattt gggaacagat gctaatagaa
    14401 caacctattt actagatggt aaaaaattca aatttagcgt taaacgtggg gagactgcta
    14461 aagtagttct tgatgttacc aactacaaac gacctgtaat tactaaggta attgataaag
    14521 aaaccgttga tactggcgaa gatgttcaat atacattgaa accatcaatt cctggtaata
    14581 ttggtaatta cgaaaaatat gttgtcaaag atgttctagg tgcagagtta acttatgatt
    14641 catcagaagt gatcgaagga atgactttag atactgatta cagtatcgaa cgtgacggac
    14701 aaacgacaaa ctatgtcttt actgaatcag gtatggataa gcttcagaaa caatttgatg
    14761 caggggatcg cttctttaca attttgatga acgctcatac taatagtaaa gctaaaatgg
    14821 atacaccact taagaatgat gtttcaacga tctacaacaa tggatatgaa cccgaagacg
    14881 aaacaccagt tgatcacgct tatacaatca ttggtgatca ccaattcatt aaaacagacg
    14941 gagatacaag caaagcattg agtggagcaa aatttgtagt gaagaacaaa attgatccga
    15001 ataaagttac ttacctaaaa caagatccaa caacgaaagc tgttacttgg attgcagata
    15061 aatctaaagc tactgaattc gtttccggat cagacggtaa gttccaaatt gaaggtttgc
    15121 aatataccta tgatgtagat tttgataaac aaggtaatcc aacaatcaca gataaaaaaa
    15181 tcaatgatta tgctcttgaa gaagttgttg ctccttcagg atatacacca actagaaacg
    15241 atgttccatt tgtggtatcg aatgatgatg cttctagcga agtaacagac atcaagaacc
    15301 ttaaaacacc tgaaacagtt cgtacaggtg gaattggaac agtagtattt ctagtggttg
    15361 caggtgtggc aggaacaggg ctatatgttg tctacaaacg agaacaacac aaagcttaat
    15421 gaaataaaat tccctagagt gtttttggct ctagggtttc tttttaaaaa gtgtgtgaaa
    15481 agtaggtatg agcatgaaaa agaagaatat caagttgatc atcttaaaaa ttgggatgtt
    15541 gctatgttta ggagtaggtc tatatcctgt aattactcag gcttatacct actaccaaac
    15601 tcaacaaaca ctaaactcct ataaggaaaa atatgatgaa gcttctgaaa aggagaatga
    15661 agaagtcttc aagagatatt cagaagttaa taggaaacga aaagacacag aacaaacctc
    15721 agtcacgatt cctgaagata ataacaatat tccgtcaaat ggaaatcttc agaaggataa
    15781 attaggtgaa ttagtgggtg aaattcttat accaaagatt aatgtggatt tgccaattta
    15841 tgatggtgta aacgatttcc aactatcagc aggagcagga atattaaccg gaactgatta
    15901 tccaactggt gggattggaa accattctgt tatatcagct cattcaggca ttcctaataa
    15961 aaaatatttt acagatttag agttgctcaa acttggtgat aaatttttta ttgatatttc
    16021 agggaaaaaa cttgcttatc aagtagatcg gataaaaaca attctaccaa ccgaatttga
    16081 ggatttgctt cctgtggcag gaaaagatta tgtaacactt atgacatgta cacctgttgg
    16141 agataattct cataggttac tcgttagagg ggttagcata ccttaccaaa aaagtattgt
    16201 tgagaaagaa aagaagaaag aagatttaaa ggtaaaggcg cagatgaact ggtatcctat
    16261 cgtagttgta ggggttttat taataatagc tgtgatagtc attattaaaa aaatagtagc
    16321 aaagagacga ggaatgaaaa atgaagaata aaatactact ttttttgact cttatttgca
    16381 ctttggtttt aacaatttat cctgcaaatc agctctatca tcaattcaaa acaaaagaga
    16441 atgtggcgga aatccaagag aaaattgaga aaaaacaaac tccttcaaaa gaggaagtgg
    16501 caaaaaaagt cgaacaaata aaatcgaaca gcggtaacca aggattagac aatactcaga
    16561 aaacagataa aggagtagac aatactcagg aaacagacgg tggatcattt gatattcgtg
    16621 aagctgaagg aaaggtcaat ccatacacag gagatgtttt ttcaaaaaat caaacgaaag
    16681 attttgaacc atctacctat tcaggagcgg ttggttatgt ctatattcca aaaataaatg
    16741 tgctagagct tttgtttcta ggcgcttcac aggagcattt atatagaggt gtggcacaaa
    16801 tgagtgctac tagtctacct ttatcaggta aaggtcatca aacagtaata gcaggtcatc
    16861 gtggcggtta tacgtcacca caatttcttt atgttgatca attagtagcc ggagaccaca
    16921 tttatataga ttatcttggt gaaaaagcag tttatacggt tttccgaaca gacgttatta
    16981 gaaattacgg tcagaattca acactgactg atattcctga tttagatatg ttaacacttt
    17041 atacttgtca tccatatcct cagaactacg atcgtttatt agttagaagt ctaagagatg
    17101 aaaaagcaaa atacatcacg ccacaagtaa cagttggcaa taccgcatac gctgatgcca
    17161 ttgatagcga gtccgtttca gataagacag aaactgttac atcttctaat gatttatttg
    17221 aggatgttga tcgtgccaac aaggaattag atggaaataa tgatcttaat attccggaac
    17281 aaggtagcgt agagatgcaa gacagaacgt ttgctgaaaa ggcaagttca atatttacag
    17341 gaattaccaa taaacatcag aaaatgcttt tagtcatttg gactgtaata ggagcattag
    17401 caagtctaaa ggttcttatt cttttaatca aagagtttcg aaaagcataa tgaagattat
    17461 aaaaattgat agagtacagt aaaaagggat tgatactatt attgatctct tttttatttt
    17521 gaatagagct agattcactt ttaaataaaa tcaaaactga tcttaattct aaagaaaaag
    17581 tcaagaaaaa aatacaacaa atttttttat cagaatattt atcagaatat tattagaaaa
    17641 gataaactca aaagcttaat ttaatagggt tattggttgt atttagaaat aatgtatgga
    17701 aaagctataa atcaatagat aagatgtaga aaatcttatt tgtgaatgaa aaacaagtca
    17761 tcgtaaaata ttaataggag gtgtagtaat ggtgggaata tcaaaaacag ctttagactc
    17821 attagctaaa ctatttagta gcaggtttga tggtgcagaa tttgttgaat atctaagcca
    17881 aaacgaaagt ggaaaaactc tgatcgaaac aatgaaaaaa gactatttga atgaaaatga
    17941 ctttccgaca tggacgaata ttgaagatgc tacatgggat gtagtatgta cgatatattc
    18001 tccttgggta acgattgctg attttgtttt agataaaatt ggaggggata cataccctga
    18061 tgccaaatct cttgctacaa gtgactttaa aacgttctat ggttatctaa taggtctaat
    18121 aggcaatgga atagatttga aagttacgcc aaaacaaaca cctgaagaaa ttgaaaaaga
    18181 aaaaggagag gttcaaatgg cttatgcaaa tgacagagaa aaagtaaaat tattggaagg
    18241 taaattatct gaagaagaac ttggaaaaat ggtgcaagaa atcaatgaag acagcaacta
    18301 ttttcctgaa ttgaaggtat atcaaaatga cgaagatttc tttaatgaaa tgtttagtaa
    18361 tagtccatgg gaagcagtga gtaaaacaaa tttaggagaa tggaactttc aagatgatct
    18421 agttgcattt agcaatgatg ggtatttacg atcatattca gaaagtgagt atagagctga
    18481 attaaaaacg tatgaaacgg atattctgat gagctattta aatctattag agagtggtca
    18541 gggagagtta agagataaag ttgaagaact attaaaagaa gagctatagg gattatttca
    18601 acataaacac aagcagaaaa ctactaaaca aaacgtacca tgcaaaaagt cggttaaacc
    18661 ggctttttgc tctattatga gaggagatca aaccatgagt gcaacaaccg atatggcttt
    18721 agaagcagta ataaaagata atacaggaaa tgtcgaaaac gtagaagact ttagcgatat
    18781 tgcaatcaat ctttcagcat ggatttcagc tgtaggagaa gaaaatatcg aatttgagat
    18841 caagaatatg gatgacttaa aagcaaaatt tccttttatc gcatcgaaac taagtcaagt
    18901 tgccgaggat aaatcgtgga atgaattagt tagtttttcc gaaaaaattt atgatattac
    18961 gcaagaagat gagtgtgttt catgggaaat gaataatcaa tacaagaaaa ttgacgcttt
    19021 cgtcaaatca cgtatagaag gaacagcttt tgagtttgaa ggtatagata ccgcagataa
    19081 ttggttaaat aaagaatcct ttattaaaag tatagcggat gatcttgaaa aacaatggtc
    19141 taaggaagac tatgcagagt acctaaaaac agaaagagat acagaggtga aagaaaaact
    19201 cactgaggta tgtttggatg aacatattga tcccaatatc gtaaaacaag aagactatga
    19261 agtggttatt gataaagttc ctactgattt tgaggaagtt gtaaagtatc tagttgaaga
    19321 agcattatcg caatactctt cagatgagga agtggacgaa gaaactgttc gtaccagagt
    19381 ttggcaactg gtttttgatg acaaagatta taaatatttg agtgatcaaa actcggttaa
    19441 ttggagtgta cgattattaa ttgatcaaaa tgatattgaa taaaatagag ctgaaaagct
    19501 ctattttttt tgttaaaaaa actagctatt ttattaaaaa taatttggtt caaattttat
    19561 ttttttaact tgataatttc ttgccatcct tttttaaatt ggctatatat caaggtttat
    19621 agtgggaata aataaaaata catttgacaa aatttacaat atagtttatt atatatttat
    19681 cttattatga taacacaata aaaggaatgg tggtatgaaa aagaagaagt ttagtcgatc
    19741 gttgttagga attttggttt tagttgcggt tggtcttagt ggcaagggtt actttcagaa
    19801 taacagcact caggaacatg attttcagat caaaagtagt gttcagatag gtaataaagc
    19861 agacagtgaa tcaaaagaat ttttagggat acgttatgat ccaaacaaat atccaaataa
    19921 tatcgtaaac attaatgatg ggaaatatac actgacaaaa gaagaccatg aagcattatc
    19981 agaaaaaggc agtcaaacct tttgggtaaa ttatgcagat ttagatcaac ttggaagagc
    20041 aggagatgtt gaagcgttga tcacctatga tgctgtaaat tctcactcat ctaaaaatgt
    20101 ggaacgtcca tctttcaaaa gttctgtcca tattgcagga gaatacaagg atggagtatt
    20161 tgatcaagag agagatactt gggtcggcaa agattcaaac aatgaaatca ctcagctaga
    20221 aggctataaa ggttatctct ataacaaaag tcattcatta gcatggtcgc taggaggaga
    20281 tatggaggta cacaatttaa cacttggaac gagagcgcaa aatgttggga aaaatgatca
    20341 gaatggaggg atggcttact cagaaacgat tgtacgtgat gcaatctata aaaataagaa
    20401 tgaaaaagta ttgtataaag ttgatccaat ttataaaggg aatgaattag ttcctcgtgg
    20461 ttctcatgtc gaagctttct cagttaacga taatggaaaa acggtaaacc ttaatgttta
    20521 tgtatttaac aatcaaaaag gaatcaacat caactatcaa aatggtactt gggtaagaga
    20581 ttagaaattg agaaggtgaa gaattggaaa caataagcat caatggatta gataaatcag
    20641 ttgcaaatcg aataaaaaaa ggaaatggct actatcttct gaagaagggt aaatattcag
    20701 ttgctagaga gttagaaaca atgagtatcg tgtatgtagc taagaatcat tttgacgtag
    20761 aacaggctat ttttgatcca gattctgaaa tagggaaatg tgttgaatta tcagagaaaa
    20821 ctgttagtac aaaaggggcg atggtcattg agagagaaat gttagacgaa atcagagtaa
    20881 tcattcctaa agatacgcag atgtgtgagg acagcataag tgacgatgaa ccaaaatcag
    20941 aaagaaaata aggagatatt cagatgagcg aaaaatccat tgaattaaag gaatataagc
    21001 gaagcgatgg agaaaaatat tatcctgtag ccagtttttc taaatcagta tatctggttg
    21061 gacgtgcatt ggtagttggt gcaattggca tatatatttt gcattgggta acaaaggttg
    21121 tacaactgct tgtagatacc ttagtaagta ttcttgtctc gttaaatgat gttaacggaa
    21181 cagatgattt aggattaaaa gagatgttta aagagttact caatgcgttt tttcccaatt
    21241 atttaagtta ttcatataat gtacaaagct tttttgcaaa gctacacttg gtagacatct
    21301 ctattttagt attagaagca ggaacggtat tctttgttgg atatttggtt actttgatgt
    21361 tcaggcatca ctatggagaa caaagaccat tttttgatga tatggaaagt agaaggttga
    21421 aaagagaagc tctagaagct atggatgtga agtatgatcc aaacaagatt ataggagatg
    21481 aaacgaacaa caaaaaaaga aaacgaaaca aaacgcaatt agaatccgat gtgctatcaa
    21541 cgcttaaaaa tatcaaaatt tctgttcata caagacgtga tctaagtgtc aatgaagata
    21601 ttcgagaata tatcgtaaaa atcccgcaac caaaagggaa gaaagttaga gaaacagttt
    21661 tacgacaaat tcaagattta gggtcgatcc tgacaagagc aacaagcgga cgagccaaat
    21721 ttggtgaaaa agaagatgaa gtttcaggcg attatttcat tttccgagca tcagtaaata
    21781 ttgaaagaga acgtaaggaa agagtaagta ccaaaaacaa agaaacggaa agagcaacat
    21841 cttcaatcgc agtttcaaat tcacaatttt cttttccaat caatctcttt gccgataatt
    21901 ccaaaaatat ccaaaaccaa accgaaaaag ctagaaaata tgccgaaact caaaagcaat
    21961 ctttagaact ttatctatct tctgctgacg tacctgcaag tctaacagat atgcacgttg
    22021 ggaatacatc tattgaatat ttgtttactt tatctcgtac cagaaaaatt caactaagct
    22081 caatgaaaga agaaattgaa acttacttgc atatatcagg tgtaatcgtg cggatcaacg
    22141 cagggcgtat tcaaattact ttaccattag gagaaaatga tcgtattcca attgatgtac
    22201 catcaatgat caatagcgtg ttttagaaga catcacaaat agaaacagaa aatacaaact
    22261 accagttcac aaacagaaaa gtaccgagaa aagtgaggaa tagaaatggc tatgaaatta
    22321 ttagagaaag tcaaaaagag agccgaaaaa gctgaatcta ttttgctaga taaagaacct
    22381 aatataaagc ttaatggaaa attcagtgtc aatatgttga acacaaaggc taaagaccca
    22441 acagatgcta tttttggaat taagcctgat ggcacaattg ctcatttttg catcagcgaa
    22501 atgccacaca tgttggttgc aggaacgaca ggatcaggga aatcagtatt actgaatgaa
    22561 attcttgtta cagtaatgtg tcactcaacg cctgatgaat taaaaatagg aattgtagac
    22621 ccaaaaaggg ttgaatttgg aagatacaaa aaattgccgt tcatgttggc tgatccaatt
    22681 acagatatgg atgaagctta tgattttttt gaatatcttg tgatcttgat gcatgaacgt
    22741 tacaaactga tggaaaaagc agggttacaa aacatcacgc tatataacga atatgccgaa
    22801 aaaaatggat tagatagatt tccctatgtg atcttattag ttgatgaata ttcacagttg
    22861 gttggaacac ataaagaagt agaaggtttg attgttcaat taggacaaat ggcaagggca
    22921 gcaggtattc atgttatctt agcaacacaa tctcctcgat caacagttgt cactggtatt
    22981 atcaaagcca attttccgtc acgtgtttgc cttatggtag caagtgattt agaaagtagg
    23041 attgtattag atgaaggcgg tggagaaagt ctaagtccaa agggagatat gcttattaag
    23101 cttgttgatg gaagtatggt tagagcgcag ggagcttaca tcagtaacaa agaaattgaa
    23161 acaatcttca atcaccttag aaatactatg ccagaaccag aatatgttga ttaccgagca
    23221 atcgtagctg aaactcgtgg tgagggcggt gaagacgagg attttggtgg aattggtaaa
    23281 gctcctgtaa gaaaatctgc taaaagaaat ttgaacacac ctttagcaaa tccatcatct
    23341 aaagagccat caattgaaaa gaagagtgcg tttgctatta agcttgaaaa aaataaagaa
    23401 gaaaaagcta atcagggagc taagtcacta gaaaaacttc agcaagaaaa attagaaaaa
    23461 ctagaaaaag cattatcatc atcaacttct gaaactcaat cttcaaaggg tatagataat
    23521 ggacaatcaa ttacaactaa aaacaagcct aaaaatagta attcaaatct ctcggtaaat
    23581 gttgttatca caactgataa atctgaaaaa aaacaagtga acggaactac ttcacccatc
    23641 atgcaaaaaa tgataaaggc aaaaaaacag gtgcagaaat gattatcatt agttcctttt
    23701 tgagatggct aacaccatat aaatatacag atgaaatagt tgagaagcga gcgaagaaaa
    23761 aagttaaaaa aacgatgtta actcctaaag aaatgctaga tcgtggaata ttgggagtga
    23821 aggttttaga agtaaaccaa ccgtttgaat tggataatgc gagagatagg ttgcttagag
    23881 ctgaagttga tgtcgcatac ttttatcatg ataaaagcaa gcatcaaata gctatcaaat
    23941 tcaatccaaa atcggcatgg cgataccaag caagagatga cctaaagcgt aggaagctga
    24001 aaccgatgct ctatcctgaa ggaaaagagg catttgatag aacccatatt gttcccattg
    24061 gatttcatgg tacagagtca gataaccgcc tattggtagg gtggaacagc tcacaaaata
    24121 gagttcagat caataaattt gagaagaagg taagaaaatt taacgatcgg aaaataatac
    24181 tttggtttgc caatattcag aagcaaccag atgggacagc aaagtgggaa gcggtcatat
    24241 ttgatgaaga tggcaaaaaa gtaatgtctg aagtatggca tgataaagct aaatttgttt
    24301 ggcagtagag gtgaaaattg tgttaactaa cctaaaacta tatagagagg gatttaacga
    24361 aaaagtaagt atcggtgaat ctattgaata tttaggaaag aagtacatta ttattcgtat
    24421 tcttgaaatt aaaaaacttt attttagtaa atataataat ttagcagtta gagttctcgt
    24481 ccaaaaggtt ggaaatccaa gtgactatag aagtgattac cataactata aaacagaatc
    24541 atctgtaatt aagcgttata atcaatcaaa aaatgatgag agaatattca aagtaggaga
    24601 aattattatt tctaagtatg ggatagcata cgaaatagtt tctattaaaa aattttatta
    24661 tgaatttgtt gatttggttg tagaatattc tgttcgattg atcgttccgt ggagtaaaga
    24721 agaaattaat gaggcattaa aacatgaacg aaaaagtaca ttcaaagttt tagaaggtga
    24781 aaaaaagtga aaacagacct tactaggcaa gctgagaagt gcttatggca tgaaacaaat
    24841 cgcatgggtg tatttggttg tttcgaagtc actatcggtt gggatggcag tgaaagagta
    24901 gattatataa cttattcagt agataacaca gttcgatgtt atgagatcaa agttacttta
    24961 gctgatttaa aaagcaaggc gaaacagact tttatcggtg attataacta tttagttgtt
    25021 actagtgatt tgtgggagaa aatgcaacaa gacgataaac ttatgcggaa atattactat
    25081 caaggcgtat acgtgttcaa tgaaaaatat cctagcataa taagtgttaa gaaagcaagg
    25141 aaacaaaaag tatcgctagg tacaagatca actgtattag aaagcatggt tcgttctatg
    25201 aatcgtgaag ttaagaaact ctataaagta aaaccatttt gggagatagc agaggtggaa
    25261 aaaggatgat tcaaacattt gcaaaaatga gtagaagtgc ttggattgaa aagctggaaa
    25321 atgatttgag tgttatgcga ttagctccaa agaaattcaa acgagagatt gcagaaaaag
    25381 aagcaatttt gtcggtattg aatgcggtag aactggacga accgaaaaag tcagttgatg
    25441 tacctgaaaa tgtagcagag ttactagatt attatcgtaa ttcgtcagat gtcgatttgt
    25501 tagctctgat attaacattt cacgattggt attactgtca aaatgaagat actcagcatg
    25561 ataaacatga taaagcagtt gactggttaa tagagaatcc agaaaagttc atgcgtgctt
    25621 ggctagatgg ctatgatagc aagaaaaagc cactggaaag taatgaggaa acgtatttaa
    25681 cggcagaaca atatgactat gctagaaaaa caggagattg ggaatctggt aagactttca
    25741 atcacagaaa agatcaacca aaaattagtt tttcagatat agccgcaatt atcgaaggta
    25801 tctactatgg acaagccgat gaagagatct tttctataca agatttatta gaatttttga
    25861 acaaaaataa ggatagcttt gtagaactaa ctgattatga ggaggataca gcaaatggaa
    25921 aattatgatc caaatatgcg atggggtaca catacgctta aagtttcttt tcaacgttgg
    25981 gattacaagg gatttgtgac ttttagaaaa gctggaaatt gtaaaggatt agatgtctta
    26041 gcgttggatg aagactatct atatgaccat ccattgacgg ataatccaat cggatttgga
    26101 ttgcttcctg aagatgatga gggcaatgaa tggttcaaaa tgattcttac aaacgataaa
    26161 ggtgatcaat tatttgtaga agatacttgg tcatatttga gtgaatatat tgttagcatt
    26221 aaaatcattg attttgtagc agataaagaa aaagagattg gagaaggtaa atctaattac
    26281 tagctaatga atagcaactt tggaggataa gtcaaaagaa taaaaaaaga atactgtgtg
    26341 tcattgcagg gatgtatctt tgcatgatag tatctgcggt taccatttat tggttaggaa
    26401 atcagcaaaa cagtttaatt gttgatctgg gggagttaaa ggttggatta cgaatgacta
    26461 gatggctttt attagctcaa acagttggtt tagccatatt gacttggata tgtgtggaaa
    26521 aaataacatc taataataat caagagattg ttgaatcaac aaacctaaga ataagaataa
    26581 ctgaacaagc aattgaaaag caaaaagaca aagaaaaaaa ggaaataatg tcaatttatt
    26641 tatctagtgg agcatcattt tttcatgaaa tagattcgat gaaactgagt agtcaagaaa
    26701 tcattgatag atggaaaaac atgcctctag gtggtgagtt aatcgttgaa gatagtgatg
    26761 gttggtcagc ttataagaag gacgagattg tagctatcac gattttgttt gaatagtaat
    26821 atattaccac tagataatct gaatatgttg atctgctagt gacgtaccaa atgcgtttaa
    26881 tagaaccttg gtcacaggca gaaattgata aagcagtaaa attgaaccga ttatcaaaat
    26941 ttaacgtatt aggaaatgcc ttttaaaaat cgcaatacac aaacagaaaa ataccaaaaa
    27001 attagcaatg aacgtaggta agaacatgac aataaataat aataacccta acaaatataa
    27061 tgctcttgtc caatgctata tcgaaaacaa aattacaatg caagaagtcg ctgatgaatt
    27121 aggcaaatca aaaagcctag tatcacacga ctttaagcac aatgtttttc cgaacccgat
    27181 tgagctgagg aaaaaatttc aaaagagagt ttatcaagaa atagggaagt ggattgatga
    27241 agggaatgca attgaccctt taattgaaaa atatcctttt ttaaaaagta aaaataaaat
    27301 tcaaaggtta attcgtcaag gcgatattca agctaaagaa cctgttttga caaccaatga
    27361 tttggctaaa ataaatcttc ataaacagat tattgaagac tttgaaaaaa atctttcaac
    27421 aactgaaatt ttaaaaaaat acaatattcc gagaggaaag ctaacatcga ctctttttag
    27481 agaatatgga gaatcggcag ttactaaagc aaaggaagta gcgaatgaga gaaaaaaaca
    27541 aaaatagtag tcgataccta gtagcgactg tagtttttgt ttgtgtattg attttcctgt
    27601 ttgatttttt gcagattgga aaaagcataa ttggaaatac attgagtgca aaggaaatgg
    27661 ttgctttagg tggtttgtat gaaggacatt tgttcccgaa tatattcatt tctatcgttt
    27721 ctcatattaa cttgtaccat ttaatttcta atttattgtt cttagtagtt cttgaagaag
    27781 cactgaatga tcaatatgag tggttaacac tattgattgt tttaatcata tcaggacttc
    27841 taggaagctt agttcctttt ctgttacaaa gcaacatcgt aagtatgggg cttagtggag
    27901 gtatttttgg atggattgct ttatttgcga ttgcatacat ccgctttgat agtaaggaac
    27961 atttattact aagtgttttt atattattga taatcggcat tttactggta tctacttttg
    28021 aatcaggcga gacaaacgtc ttgtcacatg taatcggatt tgtctgtggg atgatcttgg
    28081 gatttattct aaagaacatt tcattgatca agcaaaagaa ttaaaaggga gtgctaaaaa
    28141 tgccaaaaaa aaaagaaaag aaaattccag tgtttttgtc tgtctcagaa atgttatcac
    28201 ttcaaatact gttaagaaaa aagattgaaa caatagacga agaagttaga aataaaaggg
    28261 ctatgtacga acaacgtgaa gcgctaagag aacgaatcca ctattttgat gatatacaca
    28321 ctaaaatcta tgaagcacgt acaaataacg aagacgaaag caaattagga ttagctcaac
    28381 aacgaatatt tgaatggcta aatacgaata ctgagcaaga accgttaaga gcaattagta
    28441 atctttttga atttggaacg aagcattggt ttaacgcagt agaaaaggat gctgacttac
    28501 aagaagcctt tgacacgcta agtaaggata gcaaatccat gcgagattta ttgattactt
    28561 atctaaatga gcatgagttt gaacaaacga aaaccaaaaa gaaaaataaa gaatagaaag
    28621 ttgtgatgaa tcatgctatt aaccctgtta ggttatttat gtttaattct tgcttttttt
    28681 ggggcaatcg ctataattat tggtcaattt caaacaaata atccgattat gcgtaacata
    28741 ggcattggaa ttgtgattgt aacctttatt gtgtttaatg ccactaagat ttttggaagt
    28801 aacgataaac aatttggtat atcgggagtt aataatagtt atgctccaga ttctttggga
    28861 agggtagtaa tagatggaac atttgaaaat gtaacgagtt ataaaaatgt ttcgctgaca
    28921 gtgaaggaag atggcaagaa agcatcagac gacgttctcg ctactattaa gccgtctcaa
    28981 tcacaatttg aagtgtttta tacagttaaa gagaatagcc ctgtacacaa gctgacattg
    29041 gtttttaacg ttgatggaga agaatactat aaagatgtaa aaataaataa aacatcaacg
    29101 aaaaagatta tcgaatcagc gaaaaaagaa gtagctacag aagaaagtac aactgaatca
    29161 agttctactg tcgaagcgga actaccagat aaaattgatt ttgatcctga aaaagtcaaa
    29221 tcgtttgaat ctgtttctgg ttctatggct gatcggttta acactgaagt cttcaaagct
    29281 atcaaaactg ttaaattgga tactgaaaag caaatgatta atgttacctt attcaattca
    29341 gttgaatcta tggatcagga taaaaaagtt acggttgcaa gagaactagg aaaaagagta
    29401 attggtttgt atcgtggtac tgctaaacca gattcagacg gtgcagattt gccagttcat
    29461 gtagtttatg aagacggtaa agtttttgga gattctacag caaacgaagc attaacgatt
    29521 aaattggcta aatagccaga aagttataat aaacaaatgg tgtcacacca ataatatata
    29581 ggtgtgacac cttggagatg atagctgatg gtagtaaata aagaaaaagt tagaaagtat
    29641 gtgactttaa aaatagcttc tgttaaatat ttagaaaagc gagctaaaga agaagaaaga
    29701 gcaagaggta agaaagtaac agtttcagag ttgttagaag aattgatcga gaatgatcaa
    29761 acaataagac gtgcatttac taataggtct atcagataac aatataatta ataaaaaaga
    29821 aagagtgatt tggtcttaga ctaaatcact ctttttatat aaagtttctt tacactcatt
    29881 ccgaataatg ttaaaattct ttatatgata tatagttcct tgtcagagga agagataacc
    29941 agcaatattg gagggataat aagtggaaaa caataatgat attagacatt ttagcattgg
    30001 ggaaagagtt acaacagtaa gtgaattatc aaaggattat agagataaag agggaacgat
    30061 tatttctctt aataagaatc gaagaggcgt ttggcaatat gttgttgatt taggtgatgg
    30121 cgttacagtt gagtgtaaag atagtcaact gatcagaaat aatggcagtc attccattga
    30181 aaaagcaatt cttgtatacc caaaagtatc aagtaagatc atgaatgact ggttgcttgg
    30241 aaaatttggt gagtacaata aaaaagaaat cgcagaagtg atcggcaaat cttatccaac
    30301 agtattaggg tatctaaaaa agataacgag taaagaagca aaaattgagg atgttttaac
    30361 agtaagagag cttcaatcat taatcgagta tcggaataaa caaataacaa atcctgagta
    30421 ccttagaaaa gaagatggtc tattgaaaca attggaacaa gtaaataaaa tccggaagat
    30481 gcaaaaggta gctcctttaa gccttgatac ctttaaaaaa atctatttga aagagatcaa
    30541 aaaagactaa tgtggactgt aactttaccg ttccaacctg tatctgcacc aagaccaagt
    30601 gcaagaagga atgctgctga tgaaactaat gacgtagcag agaaaaggat ttcaacctac
    30661 tatgataaaa agtatcttga ttatttgaaa aaaataaaga gctttatcga agatcaagat
    30721 ttattagatg atgaatttta ctcgattgta aatgatccta tgggcgcaat tatggaagtg
    30781 gacttttact accagatacc aaaaagctat aaaaaagtct ataacattat gaagacaaca
    30841 gcgccagatt tagataattt agtaaaaagt gctatggatg gtatttttaa catctcagct
    30901 atacgagata gccatatcac aggcttaatt gcttttaaat ataatgtggc gaacgaagga
    30961 aaaacagttg tgaggatcaa acgatattca gaatttgagt gggatacttc tgaaggattg
    31021 aacggagata tatgggaagc tacatttgat tttaaaccag tagcaacgcc aagaccaaag
    31081 tctaaattta gaggaaatgg gattatcact tactatccaa aagagtacaa cgactatttg
    31141 gaaaatatca agaatacgct taaagagaaa gatttggtaa atgatcaact gaaaaaggta
    31201 atgaatgctc ctatgggggt tatagctcag atagattatt tttaccaagt gagaaaagat
    31261 aagaaaaagc ttgattcact gatgcgtact tcacagccgg atattgataa tttagtcaaa
    31321 ggaacattgg acggaatttt taacaaggaa ataggcatca aagatagcag ggttgtaggg
    31381 ttaattgctt ttaaatacaa tacttttgaa aagtcgaaaa caaacgtgag actacaggag
    31441 atgggctgat gaataataat gaaaaaattc ttcatgtact tgatagcttt gaaataattc
    31501 aagaagaact aaaaaaatat cgggatgttt tggaacaaag atatgatttt gtcaatcagc
    31561 aaaagagtaa tcacatggat ttcattctca atatgaatga cttgaagaag aaattagttg
    31621 aacgaaaaga acaagaaaag ttgatcaaag cttactttga actcggtgaa aaagaagtta
    31681 aaaatgcaat ggaactgaat gaagagcgca gggttttaga tcagctgtta gaacaattat
    31741 tggtgatgtt tcaaaaagga cgaatagatg aggatttaat tgaagaagga ttgagaaaat
    31801 atccttcaaa ctcaggtatc ggaatagtgc tgaaagcgat tgatgaagaa gaaattgaag
    31861 ctttcatacc gccagaggat tttgaatcgg caatggaata tataaaatat tatagccaag
    31921 ggatcactgc ttttagagag tttgatcctg aagatgttat tcatgacttg aacaacctaa
    31981 aagaatggtg tgaaagttat ggagtcgatg attccggatt ggactacttg atcagtatta
    32041 tggaaatcga agaagagatg ccggataagc ctgatccaac agatatttta gaattgatcc
    32101 atgaagctag aaatccaatt gcctatattt ctagaggata tactgtattg gaatactata
    32161 agccttatat atcagcgatg aaccatttaa gacgtgtatt aagggaaaaa cgtgaatacc
    32221 gctcagtgtt gaatgcaacg aatagattag aaaaagctgt ttcagagcta gatgcttatt
    32281 atcgtgaaca ttacctgcaa gcaggtggta tgccaagaaa tacgaaagca aatatcagta
    32341 ggtatataaa aaaagctgag taggcggagt tagcccataa aatggcaaaa ggtaacctga
    32401 aaggggcatt ttgaatgaaa agggatgttt ttgaattgta cgtattagaa cgtctagaag
    32461 acggcatgat ggcacaagag ataattgatg aaattgatga tatgatcagc agatttggtc
    32521 aattggagtt gaatgatgga aacaaaaatt cggtagtgct aaaacaattt tggttgaaca
    32581 atttttcgta taaggatttt atcaaagaaa aggcaaatac attgtactga ctaaattcac
    32641 gaggagcagt gatgagagct tattgctaag agcagttagg ctcttttttg tttgtgtaaa
    32701 attatttgtt ttataataca catttataca caaaaaaata ttgacagata gtacacaaat
    32761 gtggtaggat atatttgtaa gcaagttaga ttaacaagtt aaaggaagag cagttatgaa
    32821 aaaagaagaa ctaatagata tatttcaaat tgttgaacga gcaaataata tgggaattat
    32881 gttttttgat cgtatttctt taaagatg
//
LOCUS       DANKEF010000028        32533 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000028 DANKEF010000000
VERSION     DANKEF010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32533
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.028"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            131..505
                     /locus_tag="PWZ48_001478"
     CDS             131..505
                     /locus_tag="PWZ48_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2261142.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="PWZ48_001479"
     CDS             complement(619..1248)
                     /locus_tag="PWZ48_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2261143.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="PWZ48_001480"
     tRNA            complement(1382..1454)
                     /locus_tag="PWZ48_001480"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="PWZ48_001481"
     tRNA            1668..1741
                     /locus_tag="PWZ48_001481"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="PWZ48_001482"
     CDS             complement(1879..2043)
                     /locus_tag="PWZ48_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261144.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="PWZ48_001483"
     CDS             2338..3666
                     /locus_tag="PWZ48_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HDL2261145.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="PWZ48_001484"
     CDS             3868..4701
                     /locus_tag="PWZ48_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDL2261146.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="PWZ48_001485"
     CDS             4717..5454
                     /locus_tag="PWZ48_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HDL2261147.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="PWZ48_001486"
     CDS             complement(5609..6577)
                     /locus_tag="PWZ48_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDL2261148.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="PWZ48_001487"
     CDS             6809..7642
                     /locus_tag="PWZ48_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDL2261149.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="PWZ48_001488"
     CDS             7676..8515
                     /locus_tag="PWZ48_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2261150.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="PWZ48_001489"
     CDS             8541..9101
                     /locus_tag="PWZ48_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2261151.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="PWZ48_001490"
     CDS             9300..11021
                     /locus_tag="PWZ48_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HDL2261152.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="PWZ48_001491"
     CDS             11186..12328
                     /locus_tag="PWZ48_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDL2261153.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="PWZ48_001492"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="PWZ48_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDL2261154.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14775
                     /locus_tag="PWZ48_001493"
     CDS             14128..14775
                     /locus_tag="PWZ48_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HDL2261155.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    14904..15120
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15168..17813
                     /locus_tag="PWZ48_001494"
     CDS             15168..17813
                     /locus_tag="PWZ48_001494"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDL2261156.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18123..19436
                     /locus_tag="PWZ48_001495"
     CDS             18123..19436
                     /locus_tag="PWZ48_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDL2261157.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19426..20079
                     /locus_tag="PWZ48_001496"
     CDS             19426..20079
                     /locus_tag="PWZ48_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2261158.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20134..20826
                     /gene="radC"
                     /locus_tag="PWZ48_001497"
     CDS             20134..20826
                     /gene="radC"
                     /locus_tag="PWZ48_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDL2261159.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20976..21176
                     /locus_tag="PWZ48_001498"
     CDS             20976..21176
                     /locus_tag="PWZ48_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2261160.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(21430..23109)
                     /locus_tag="PWZ48_001499"
     CDS             complement(21430..23109)
                     /locus_tag="PWZ48_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HDL2261161.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(23111..23323)
                     /locus_tag="PWZ48_001500"
     CDS             complement(23111..23323)
                     /locus_tag="PWZ48_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HDL2261162.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            23716..25446
                     /gene="efrA"
                     /locus_tag="PWZ48_001501"
     CDS             23716..25446
                     /gene="efrA"
                     /locus_tag="PWZ48_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HDL2261163.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            25443..27203
                     /gene="efrB"
                     /locus_tag="PWZ48_001502"
     CDS             25443..27203
                     /gene="efrB"
                     /locus_tag="PWZ48_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HDL2261164.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            27298..27495
                     /locus_tag="PWZ48_001503"
     CDS             27298..27495
                     /locus_tag="PWZ48_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261165.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            27648..28133
                     /locus_tag="PWZ48_001504"
     CDS             27648..28133
                     /locus_tag="PWZ48_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDL2261166.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="PWZ48_001505"
     CDS             complement(28237..28830)
                     /gene="clpP"
                     /locus_tag="PWZ48_001505"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDL2261167.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            29010..29537
                     /locus_tag="PWZ48_001506"
     CDS             29010..29537
                     /locus_tag="PWZ48_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261168.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            29628..30365
                     /locus_tag="PWZ48_001507"
     CDS             29628..30365
                     /locus_tag="PWZ48_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2261169.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            30369..31286
                     /gene="miaA"
                     /locus_tag="PWZ48_001508"
     CDS             30369..31286
                     /gene="miaA"
                     /locus_tag="PWZ48_001508"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDL2261170.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            31288..32517
                     /gene="hflX"
                     /locus_tag="PWZ48_001509"
     CDS             31288..32517
                     /gene="hflX"
                     /locus_tag="PWZ48_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDL2261171.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT        10772 a         5522 c         6874 g         9365 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cggggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggctatga tgtccctttt
    14341 cttatcgatg tattcaataa tatccttaat actaaagaag aaaaacgaat cgcattagta
    14401 ggatatggaa atcttggcaa agcattgaaa cacaacaatt ttagaagaaa tgaaaatcta
    14461 aatatcgtgt gcgtctttga taatgatcca gcactgatca atcgagtaat tgatggagaa
    14521 atgatctatc cgattgatcg tttcgcggag atagccaaag caaaaaatgt gacagtagca
    14581 atctctacgg taccaagtaa atattcacaa tctgctattg atgaaattgt caaaggaaat
    14641 gtaaccgcta tacttaattt tgcgcctgac agagtaactg tcccagctta tgtcaatgtt
    14701 caatacattg atttgacaac cgagttgcag acgcttattt actttgatga gaattactca
    14761 gaagtttttt cttaggaaaa tttgagtagg cttattgatc ttgcgagaac tcgtcgtaat
    14821 tctttcaaaa attttttagt actaaatgaa gtggtttttt cacgaccact tgttgactaa
    14881 aaaaaaaaac cattgtagaa tgaagcataa cgctgaacaa gaggagtaac tggcacattc
    14941 attcttagag agagaactat ctgctgaaag gttctcaatg acaaccagtg aaggtagctt
    15001 gggagtttct gtcttgaatg gagtagagat agacgagtcg tgatcgttaa ctcacttgag
    15061 aggcaataat gattgattat tgcaatttag gtggtaccgc gtaaatcata cgtcctagaa
    15121 tacgaaagta ttttaggacg ttttttagtt taaaggagga aaacagcatg acggaagaaa
    15181 ataacctatc aacaaaatat aatccgcaag aagttgaagc tggacgttat cagcattggt
    15241 tagaacaaga tttgtttaaa ccgagcggag ataaaaaagc aaaaccttat tcaatcgtaa
    15301 tcccgccccc aaatgttact ggaaaacttc atttagggca tgcttgggat acgaccttgc
    15361 aagatatgct gattcgtcaa aagagaatgc aaggatatga tactttatgg ctgcctggta
    15421 tggaccatgc aggaatcgcg acacaagcaa aagtagaagc gaagcttaga gagcaaggga
    15481 tttctcgtta tgatctaggt cgtgaaaagt tcatcgatca agtgtgggaa tggaaagaag
    15541 agtatgcgag ccacatccgc gaacagtggg ctaaaatggg cttatcttta gattacagta
    15601 gggaacgttt tacattagat gatggattat ctgaagcagt acgtaaagtt tttgttacct
    15661 tatacgaaaa aggtttgatt tatcgtggtg aatatatcat caactgggat cctcaagcac
    15721 gaacagctct atctgatatc gaagttattc ataaagatat cgaaggcgcc ttttatcata
    15781 tgagctatga actagcagat ggatcaggcg tagtagaaat tgcgacaacc cgtccagaaa
    15841 cgatgttggg agatacggcg attgctgttc atccagaaga cgagcgttat caagcgctca
    15901 tcggcaaaaa agtgattcta cctttagtca ataaacaaat cccaatcatt gcagatgaat
    15961 atgtagatat ggaattcggg acaggtgtag taaaaatcac tccagcacat gatccaaacg
    16021 actttgaggt aggtaatcgt catgatcttc cacgcgtaaa tgttatggat gatgatggta
    16081 cgatgaatga actagcagga aaatatgctg gaatggaacg ttttgctgct cgtaaagcaa
    16141 tcgtgcaaga tctgaaagaa atcggtcgat tgattaaaat cgatacgatg atccacagtg
    16201 tgggtcattc cgagcggaca ggtgtcgttg ttgaacctcg gctgtctact caatggtttg
    16261 taaaaatggc gccgttagcg aaaaaagcca ttgataacca agaaacagat caagcagtgg
    16321 aattcttccc tccaagattc aatcaaacgt ttttacgttg gatggaaaat gtccatgatt
    16381 gggtaatctc acgacaatta tggtggggac atcaaattcc tgcatggtat cacaaagaaa
    16441 caggtgaaat gtatgtaggg atggaagcac cagcggatag tgaaaactgg gtacaagatc
    16501 ctgatgtttt agacacttgg tttagttcag ctttgtggcc tttctcaaca atgggctggc
    16561 cagatacgga aagcgaagat tataagcgat acttccctac tagcacttta gtaaccggtt
    16621 atgacatcat tttcttctgg gtaagcagaa tgattttcca aagcttggaa tttactggcg
    16681 aacgtccatt tgagaatgtc ttgattcatg gattgattcg tgacgaacaa gggcggaaga
    16741 tgagtaaatc attaggtaat ggaatcgatc cgatggaagt aatcgaaaag tatggagcag
    16801 atgctctccg ctggttctta tcgaatggtt ctgcaccagg acaagatgtc cgcttcagct
    16861 atgaaaaaat ggatgcttct tggaacttta tcaacaaaat ctggaatgct tcacgtttcg
    16921 ttatcatgaa tatcgaagga atgaccgtag aagagatcga tttatctgga gaaaaatcag
    16981 tggctgaccg gtggatcctt actcgtttga atgaaacgat cgaacgagta actgagctat
    17041 ttgatcgttt tgaatttggt gaagcaggtc gacaactgta taactttatc tgggatgatt
    17101 tttgtgactg gtacatcgaa atgagtaaag aagtacttta tggagatgat gctgtttcaa
    17161 aacaaaccac ccgtagcatc ttagtccata cacttgatca gatcctacgt ttattgcatc
    17221 caatcatgcc attcgttaca gaagaaatat gggaacatat tcctcatcag ggaaattctc
    17281 tagtagtggc agaatatcct gttgtacgtc cagagtttga tgatgaaaca gcttctaaag
    17341 ggatggaagt attgaaagaa ttgattcgag ccgttcgaaa tattcgttca gaagtgaata
    17401 caccattgtc taaaccaatt acgttgctaa ttaaaacaaa cgatgctaaa atcgatcaat
    17461 ttttagttga aaatacaagc tatatcgaac gcttctgtaa tcccgaagag ttgacgatat
    17521 caagtgagat cactgcgcca gatctagcga tgtcagctgt tctgacaggt gcagaaatct
    17581 tcttgccgtt agcaggattg atcaatattg aagaagaaat caaacgatta gaaaaagaac
    17641 tggaaaagtg gacaagcgaa gtaaaacgtg tccaaggaaa actttctaat gaacgttttg
    17701 tttctaacgc gccggaagaa gtagtagagg cagagcgtgc aaaagaaaaa gattatttag
    17761 agaagcaaac ggctgtcaaa gaacgaatcg aaagtttgcg tactattcaa taagaataga
    17821 aaaagagagt gtgaaatcag ctagctatta ctaaaagcta gctgattgaa catcgttaca
    17881 ggaaagagtt tgagcctcac tcttagcttc gattcataag gagatgtgac ataaattttg
    17941 tcgcatctct tttttagtct actcttatta tgtaggagaa ggagggaggt ggatcatgta
    18001 tcaagagtca ataaagtagt tgataaaatc gttcctctct ctgtttcttt tctctttgtc
    18061 atacttttat ttttcttatg tgttctattt gttataataa aagagaaatg ggggaattgt
    18121 tgatgacgat tgaagaagca atagaatgga tccatagtcg attaccattt ggttcacgtc
    18181 ctggccttga ccgagtcgaa gctttattag aagtggtagg gcatccagag agaaaagtaa
    18241 aaacgatcca tatcgctgga acaaacggaa aaggttctac tgtagcttat ttgagaagcc
    18301 tattagaaga ggctgggctg acagtaggta catttacttc accttatatc gaaacgttca
    18361 atgaacggat cgcaatcaat ggcaaaagca ttccagacaa tgatttgatc cagcttgtaa
    18421 aaaaatatca gccactgatc aaacaacttg atcaatttga agcagtagct ggtattactg
    18481 aattcgagac attaacagca atggcatttg attatttttt gatgaagaaa gtggacgtag
    18541 cgattatcga agttggctta ggcggcttat tggattctac caatgtagct gtaccaatgc
    18601 tgacgggaat aacaacgatt ggacttgatc ataccgaaat acttggagat acaattgaag
    18661 aaatcgctgc acaaaaagct ggaatcatca aacaaggcgt tccagtagta acaggaaata
    18721 ttgccccaga agcttttact gtcatccaac aaacagctaa agcaaaaaat gcaccgcttt
    18781 atgtatacgg ggaaaattat aaaataaact atctgcatcc tgatgagatg tggggagaag
    18841 tatttgaatt ttttaatgaa ataggaaaga tccctcaatt aaccactccg cttttagggc
    18901 aacatcaagc tgaaaatgca ggagtagcca tcgaattgta ttttgtttac tgtcaattgg
    18961 aaaagctgcc tttccgagaa aaagatgttt tgaagggatt gaagaaatct cgatggccag
    19021 gaagaatgga gcgtttgagt aacgagccat tagttgtttt agatggtgcc cataatacgc
    19081 atgcaatgaa ccggcttgtt gagaatttga aaaaagaatt caaaggatat caaatcaaca
    19141 ttttgttttc cgccttacga actaaaaatg ttagagaaat gctagaacga ttgcagcaag
    19201 taccaaatgt ccatatttat ttgacgacct ttgaacatcc aaaagccatc gaattaagtg
    19261 aagtatcaga tttagcaagt gagagagtat caatcgtttc attgtggcaa ttcggattag
    19321 cagatatatt ggaaaagatg acaaatgaag acgtattagt tgtgaccggt tcactttatt
    19381 tcatttcaga agttcgccat ttattaatac aattaggagg agatcatgaa gtataatgca
    19441 gtgatttttg acatggatgg tttgctgttc gacacagagt tagtgtatta cgaagccaca
    19501 caagtagtag cagacaagat gggttttcct tatgataaag acctgtattt aaaattctta
    19561 ggtgtttcag atgaagaagt atgggcgaat tatcatatga ttttctcgga atttggcaaa
    19621 acaagtgtac aaagattcat agatgattca taccaggaaa cgcttgaacg tttttcgcaa
    19681 ggagcggtac aaatgaagcc cggtgtgttg gaactcctct cttttttaga agaacatcag
    19741 attcccaaag ttgtcgcttc cagtaaccaa cgagcagtca ttgaactttt attgaagaag
    19801 aacaatctgt ttgaacgatt tgagacgatt gtttctgcag aaaatgttca acgagcaaaa
    19861 ccagacccag aaattttctt gttagctaat cagaagctag gtacagcgaa gaaagaaaca
    19921 ttgattttag aagattcaca aaatggtatt ttagcagctt ctgcggctga gatcccggta
    19981 gtcatggtac cagatctgct tccaccatcc caagaattga tcaaaaaaac agctggggtt
    20041 gtttcttctt tacatgaaat ccctgcattg ttaaaataaa aatccatctc ttttcgtatc
    20101 ttattttaga taagatagaa aggagatgtt tttttgggaa aacgattgat caaagaagta
    20161 cctactagct ctttaccaag ggaacgtatg gaaatttatg gcgcggaagc tttatcggat
    20221 caggagcttt tggcaatctt actgcgtacc ggtcaacacc cttatagtgt gatgtcgatt
    20281 gctggaaatc tgttaaaaac gtttggagga ctggcctctt tacgacaggc tactttgcat
    20341 gagttagaag agattcgagg gataggacgc gtgaaagcaa tcgaaatcaa agccttgatc
    20401 gaattaggtc gacgaattca gacggatcat gaaagagcgg ctccaccggt tcgtaccagc
    20461 tacgaattgg cccagcaatt gattctggaa atgaaagatc acaaacaaga acatttggtt
    20521 tgtatttatt tggacacgaa gaatcaagtg atcttaaaaa aaacagtgtt catcggttcg
    20581 ctgaatcagt cagtcgctca tcctagagaa attttccatt atgctgtacg ttattgtgca
    20641 gcaaggatcg ttttagcgca taatcatcct tctggtaatg ttattccatc gcagcaggat
    20701 atgaatttta ccaaaaggat acaaaagtgt ggagaaatga tggggatcac tgtactggac
    20761 cacttgatca tcggacgcaa acgatatttt agtttgagag aagaaggaat gatggaagag
    20821 aagtaagttg ctttcttgca aaaaaaatga agcaagtgta caattatatt gtaattttgt
    20881 ttgggaatag aagttgaaac atctgttgca cctctatcgt acacgagcaa tgttacaatt
    20941 taaattagtg cttttggcac gaggaggata ttattatgga aaacggaaca gtaaaatggt
    21001 tcaacgctga aaaaggattt ggatttatct cacgtgaaga tggaagcgac gtattcgttc
    21061 acttctcagc tatccaaggt gacggattca aaacattaga agaaggtcaa gcagtgactt
    21121 tcgacgttga agaatcagac cgcggcccac aagctgcaaa cgttgtaaaa gcttaatttt
    21181 atctgtaaat caaattgatg gtagacatca atttttacga gagaacaaag cgagtaacaa
    21241 aaatacctgc cacaagagac aatttctctt gtggcaggta tttttttgta tgaaaaagta
    21301 aataaattta agaggctatg aaagagccac cttgtatcaa ggaataagaa caaacaaaaa
    21361 tggcttctca tgtttttcat ttgttttctc ttatattcag gacaaggtgc tgatttcata
    21421 acctcttatt tattcttcat ctggtgtcat taccattggc aaaatcattg gatgtctttc
    21481 tgtacgttcg aataagaaag gctgaagcgc gccagtcatc gcttctttca atttatattc
    21541 tgaacagtca ggtccattca aacattctct taaagcgtgg aataagattc gctgagcttc
    21601 attgatcatt tctcctgatt ctcgcatgta gacaaatcca cgagagagaa tatctggacc
    21661 agccaagatt tcttttttct tgatatccac tgtcgcgaca gctaatacaa gaccttcttc
    21721 tgaaagcagg tgacggtcac gtaaaacgac attaccaata tcaccgatcc cgctaccatc
    21781 gatatacaca tctccagcca caaaatgccc agcaggacgc gcagaatcgc tagttaacgc
    21841 taaaacatcg ccattttcca tgatgaagca gttttctgca ggaacaccta catcttgtgc
    21901 tagagaagca tgtactttca acatgcggaa ttctccgtgg acaggaataa aatattttgg
    21961 cttcatcaaa cgaagcatta atttttgttc ttcttgtcca ccgtgaccgg aagtatggat
    22021 gttattgatt tttccgtgaa tcacttctgc accagcttca gacagcaaat tgatcaaatg
    22081 attgacactt gttgtattac caggaatagg tgaacttgaa aagatcactg tatcgtctgg
    22141 ctggatttgg atttgacggt gagtaccatt cgcgatgcgg ctcaacgcgg ccatcgcttc
    22201 tccttgagaa cctgtacata ggatcatcgt ttcattggca ggcaagcgat tgatttcgtg
    22261 tgcatctaca aatgtgccgt ctggaacttt gatatatcct aaacgttgac cgttgacgat
    22321 ggcattttcc atgcttcgtc cgaaaacagc gattttacga ccagttgcca cagcggcatc
    22381 tgctgcttgc tgcaaacgga agatgttaga agcaaagctg gcaaaaatga tacgtccttc
    22441 gatttttgaa aagatcttca aaatagaatg cccaatcgtt ttttctgatt tagtaaatgt
    22501 tggaatctca gcatttgtac tatcagataa aaggcacaag actccttctt ccccgatacg
    22561 agccatttta tgcaagtttg ctggttcgcc aacaggagtg aaatcgaatt tgaagtctcc
    22621 agtcgctacg atatttccag aaggtgtttt caccacgatc cctaatgcat ctgggatact
    22681 atgggtcgtt ctgaaaaagc tgacagaagt ctttctaaaa cggatgatcg tatcttcatt
    22741 gatctcatgg agctctgcat ctcgaagcaa gccatgctca tctaatttgt ttgtgatcaa
    22801 cgctaatgcg agaggaccag catagatagg aatatttgct tgtttcagca agtagggtat
    22861 tccgccgatg tggtcttcgt gcccgtgagt aatcaccaag gcttttactt tgtgtaagtt
    22921 ttgtatgatg tagctgtagt cgggaatgac atagtcaatt ccaagtaaat cgtcttccgg
    22981 gaatttgatc ccggcatcaa tcaaaatgat ttcatcttgg aattgtaccc catacgtatt
    23041 tttcccgatt tcacctaatc caccaatagc aaagacgcct gtctcgttat ttttgatgga
    23101 aagtttcata ttaaaactcc actaatgaga aatcagcatt ttctttttcg tactctagat
    23161 gacttccttc tagaagttga acgaattcaa tattgtatgg ggtattttct tccacacgtt
    23221 ggcgagcgtc tacatcgctt tttgcctcaa tgtaaagaga ttttgtttct tcccgtttag
    23281 ggttgcgtac ttttgtttct tgataataaa ctttatagat cattgttttg tctcctttat
    23341 ctgcgtaaag cagtgattta gctagttgat ttttttagac aacacaaaac gaggggacaa
    23401 gccacctggt ttttcatatg ctttgactag aatactggat tcgagtcaaa gagacaagcc
    23461 tcgtttttgc ttatctgttc ggatattttt tcaaccgtac cttctgtata aaaaatatac
    23521 aacaattaat tgttattcta gcataaaaca gcaaataagt atagaaatca actagattga
    23581 aagaaaaaat caatgtttat catcccgcta taatcataat gactttttta tgagagaagt
    23641 gttctattaa taggctcgtt gtgctatcat ttttagtaat gaaaatgtaa caaaagatta
    23701 aaaggagatg agaaagtgaa actaatgtgg cattatacca tgcgttataa gaaattcttg
    23761 tttttcaatt tcatatgcgt atttggtttt attttgattg aattaggact tcctacgatt
    23821 ttagcacgta tgatcgatgt aggaatccgc aatgatgatt ttgattatgt gaagcaacaa
    23881 gggatattta tgatcgttat tactgtaatc ggtattattt tgaatatctt gttaggttat
    23941 tttggttcga ggattacgac taatatcgta gcagatattc gagatgattt gttcgaaaag
    24001 atccaaagtt attcgcatca ggaatacgaa aatctaggcg tatcttcatt gatcactcgt
    24061 acaacaaacg atgcttatca gatcatgttg ttcctgcaaa atattttacg tatcggtttt
    24121 atggcaccaa tgatgttcat tgtcagtttg tacatggtca tgcgtacaag tgtgacactc
    24181 agtttgtatg tagtaggtgc attaccttta ctattattgg cagtcgtagc gattgctaag
    24241 gtgtctgaac cgttatctaa aaagcaacaa aaaaacttag ataaaatcaa cagtatcttg
    24301 cgggaaaatc tttcaggatt gcgtgtgatt cgtgcattcg tcaatgaaaa gtttgaagaa
    24361 aaacgttttg ctgtagtcaa tgagaattat gcttctagtt caaaaagctt attccgtttg
    24421 atggctgttg cacaaccggg tttttatttc ttatttaata tcgtaatggt attgatcatc
    24481 tggagcgggg cagtgcaaat cgatgcaggc gagcttcgtg taggagattt gattgcgttt
    24541 atcgagtata tattccatgc cttgttctca ttcatgttat ttgctagtgt gttcatgatg
    24601 tatccaagag cagctgtttc agctggacgt atccaagaag ccctagatgc caaaccttcg
    24661 attgtagaag atccaaatgg tatatctgat acaaaaacta aagggtatat cgaatttcaa
    24721 aatgtaacat ttgcttatcc aggccattcg caagagccag tgatacgaaa tgtcagtttt
    24781 acagcctctc caggagagac cgttgctttt attggaagta ccggtagcgg aaaatctacg
    24841 ttgatccaat tgatccctcg cttttatgat gtgagtgaag gaaagatctt gatcgatgga
    24901 gtagacgtga gagattatcg gctaagcaag ttgcgagata agattgggtt tattccgcaa
    24961 aaagcgctgc tatttactgg aacgattgca gataatttgc gatacgggaa agaagatgct
    25021 acgcaagaag aaatggaacg tgcagctgag attgctcaag cttcagaatt catttctcga
    25081 aaaccagatg gatttaatga acatttatca gaaggtggtt ccaatttttc aggtggacaa
    25141 aaacaacgat tggcaatagc cagagccatc atacgccgtc cggaaatcta tatttttgat
    25201 gacagctttt ctgcattaga ttatcaaacg gatgcgaagt tacgtgctcg tctgaaaaaa
    25261 gaaacgacag aatctactgt attgatcgta gcacaacgtg tgggaacgat catgcatgca
    25321 gataagatca tcgtattaaa tgaaggagaa gtcgtcggta tggggacgca tcgggagttg
    25381 ttggaaaaat gcccgattta ttatgatatt gccgcttctc aattatcaaa ggaggaatta
    25441 gcatgaaaaa aaccttttct tccttaaaac gtttagctcg atatatcaga ccgtatcgtg
    25501 agacttttct tttagtgatc ctatttacag ttctgacagt ggctttcaac gtggctttgc
    25561 catatgttac aggacttcct actacagaaa tcagccgtaa tattgccaat ggcgaatcaa
    25621 tcaatttttc ttatatattc aaatgtctca tttggatcac agttgttgga atagggtatt
    25681 gtatttcgca attactttct ggtgttttga tgacaaatgt cgttcaaagt gccatgcatg
    25741 atttgagaag agacatagaa gaaaaaatca atcgtcttcc ggtttcatat ttcgataaaa
    25801 accagcaagg aaatattttg tctcgtgtga cgaatgatgt agatgcagtc agcggtgctt
    25861 tacaacaagc ttttattggt atcgtaaatg cgatattggg aattacgttg gctgcagcaa
    25921 tgatgtttta tatccagccg atgatggcgc tgatctctat gatcatgatt ccaacatcga
    25981 tctggatctc aaaacgagtg atcaatgctt cgcagaaata ttttcaaagc atgcagaatt
    26041 ctcttggtga gctaaacggc tacgtccaag aaaatatgac aggttttagt gtattgaaag
    26101 tatatggacg cgaaaaagaa acattggaag gattcaaaaa agttaatcac agcttgaaat
    26161 actttgggtt tcgtgcagca ttcatttctg gcttgatgat gcctctagtt caattaactg
    26221 cctatgctac ttatattggt atggctgttc taggaagctt ttatgttatt actggggcga
    26281 tcgttgtcgg tcaactacaa gcattcatcc aatatatttg gcaaatcagt cagccaatgg
    26341 ggaacgtgac acaattgtct tcagttttgc aaagtgcatc agcggcaacg aaacgtgtat
    26401 tcgagatttt agatgagcca gaagaaaaag aaaacaaagt agatgcttca ctgccagaaa
    26461 cgattttagg agatgtgaca ttcgatcatg tcgattttgc atatgatccg aagaaaccat
    26521 taatccaaga tttgaatttt gaagtgaaag ctggtcaaac cgtagctgtg gtcggtccga
    26581 ccggtgccgg caagacgaca ttgatcaatc tattgatgcg tttttatgac gtagacaaag
    26641 gagcaataag gattgatgga atcgacacca aatcaatgag tcgcagtgat gtgcgttcgc
    26701 tatttggtat ggtcttgcaa gatgcatggc tttatcaagg aacgattgca gataatatcc
    26761 gcttcggaaa gcttgatgcg acagattatg aagtagttga tgctgcgaaa acagcaaacg
    26821 tagatcattt tatccgaacg atgccagatg gatatgatat ggccatcaat tctgagggag
    26881 acaatgtgtc tttaggacaa aaacagttgc tgacgattgc ccgagcagtg gtctccgatc
    26941 ctaaaatctt gattttagat gaagcaacaa gttcagtaga cacccgttta gaagcgttga
    27001 ttcaaaaagc aatggatcgc gtgatggagg gacggacaag tttcgtcatc gctcaccgtt
    27061 tatcgacaat ccgtgatgca gatctgattc tagtgatgga tcaaggtcag atcattgaaa
    27121 aaggaacgca tgagagttta ttggcacaag gtggatttta tgaaaaactg tataacagcc
    27181 aatttgctga ggaagcagaa taagtcatat aagaataaaa cagtttaaaa cttatttttt
    27241 gttctctatt cttgataatg gtaaagtaaa gttatcaaga aaagtgagga cacggagatg
    27301 gaacagtttt taaatgaaaa aattactgaa gccaaaagag aagttgatcg tttaacgcac
    27361 gtctgtcaga cgaagccttt tgattcagct gatttttttg aaaatgagtt tcaaatcgaa
    27421 cgcttggcag cacaaataga agtgtacgaa ttaattttaa aaaaagtaat agaagaaaat
    27481 atagatgtac aataaaaaca ggtaaaacgt cagttaccag atactgtaaa tagacatctg
    27541 gcctgagcag gttttacctg ttttttttct tatgaaataa atatatgcta tgataccttt
    27601 attcaatagg attagtttct acaaaaaatg aaaggagaaa aataaatatg gaacggttta
    27661 tagcatcaaa tgccacagtg attggtgatg tgacattgtc agaagatgtg acgatctggt
    27721 atcaagctgt tttacgcggg gacagtaatt ggataaaaat aggacaaaga acgaatatcc
    27781 aagatggtac aatcatccat gtcgatcatg atgcacctgt agatattgcg gagaatgtga
    27841 cggtaggaca tcagtgcatg ctacatggct gtacaatcga aaaaggggcg ctgatcggga
    27901 tggggaccac gatattgaat catgcggtta ttggtgagaa tagtttgatt ggtgctggat
    27961 cattagtaac agaaggaaaa gttattcctc ctaatgtctt ggcttttggt cgcccagcta
    28021 aagtgatccg tcctttgaca aaagaagaaa tacaaaaaaa caaagaaaac atccagcatt
    28081 atgtcgagat aggtcggatg catgcgcaag gaaaattcaa ggagcaaaga taggcagtag
    28141 catccataaa ataaaaaagg atggtcagtt tgagttgacc atcctttttt atttggttta
    28201 gataagagag cttaaaaaag cttcctttga aacagattaa tttaatgaag aactgttttc
    28261 catgacttca tcgatcaatc catatgcttt tgcttgttca gctgtcatat agttgtcacg
    28321 atctgtatct ttttcaatca cttcaagcgg ttgtccagtg cgttcagcta aaattttgtt
    28381 taagcgttcg cgtgtttgca aaatatggcg tgccgcaatt tcgatttcag ttgcttgccc
    28441 ttgagcgccg ccaagaggtt gatggatcat gatctcagca tttggtaaag caaatcgttt
    28501 accttttgta ccagctgtca gcaagaagct tcccattgaa gcagccatac ccataacaat
    28561 tgtttggaca tctgctttga caaagttcat tgtgtcgtaa atagccatac ctgctgtaac
    28621 tgaaccacca ggagaattga tataaagata gatatctttt tcagaatctt gagcgtctaa
    28681 gaaaagcaat tgggcgatga tcgaattagc aagatcatct gttacttggc cgcttaacat
    28741 gatgatacgg tctttcaata aacgtgagta gatatcgtaa gctctttcac ctcgtgaaga
    28801 ttgttcaatg actgtaggaa ttaaattcat aatagagttc ctccttgatt tcgtttagaa
    28861 atagtgtacc ataatttagt ttctaatacc taaaatgtag tatacatttt tggtcaaaaa
    28921 aggtcaaata aaaaacttag gcaaattagt cgcataaacc cgctttatgg tatgataagc
    28981 agagaaacaa aacaaggagg aacaacagat tgatagttgg tattactggc cttatcggag
    29041 tgctgacagg tgctgtagtt gtctatatcg gcatgatact gcaaatgcgt cacttggaaa
    29101 ataaagaaca gcgtagacgt gaattggaat tgaaagtcaa agaaatcgag acgctgaatc
    29161 aattaaataa aaagatcaac gagatcttgc aaaaacggac aacgctgatg gaagaatatg
    29221 tcagctttga tgcatttgat gattgttata tcacgattga cgattttgcg tatctccaat
    29281 cattttcagc gcaaaatagt ttttatctgc caaattatat tttagacgag ctatttaaga
    29341 atatctccca tcgaaaagtg attttatctc cagatgaaac aatgaagatt ggtggatatg
    29401 cttataaagg cggacgcgtg attttagaga attttacgga tagtctgact gaaatgatca
    29461 atgaaaaaaa agctcagttg aagactatga cgcgccagcc gattggtttc ttctcgaaag
    29521 atgatgtttg gagctgatat tttgaagcat tgccgttgca catgagagcg tggtaatgct
    29581 ttcttttttt cttaatgata caatagaact gaacggagtg aagaaagatg ttacaaatct
    29641 atgcccatcg cggaagcagc ggcacccacc cagaaaatac gcttcctgca tttgcagaag
    29701 ctgtacgagt aggtgcagat ggtatcgaac tagatgtcca tctaagcaaa gatggttatt
    29761 tgatcgttat gcacgacgaa gaagtggacc ggacgactaa cggtaaaggg ctgattcgag
    29821 aaaaaacatt ggaagaactc aaaaaattga atgcaggggg caaattcagc aaagaatttc
    29881 cagcagctaa agttcctgaa ttgagagaag tgttaggact gctcctccaa aaaaattacc
    29941 gagggatgct aatgattgaa attaaaactg atcagtatga atatcccggt atcgaagaaa
    30001 aattagccga aacattaaaa agtagagatt ggccttttca acatgcatat tgcagctttc
    30061 atttggattc tttggaaaaa atcgcattac tggaaccgga gatacagctg gacttattga
    30121 tgagtacttc tgataaaaag atccaactag cagcagaaac cccttatata gaaggagtac
    30181 atccaaaaaa tacttggttg ttcgaacatc cagtccaggc agctgatttt ccaaaagcaa
    30241 ttcgtgcatg gacgattaac gatgaagaag aaatggccaa atgtcaagaa ctcaaaatag
    30301 atggaataat aactgatttt ccggagaaag ctcttcggtg gaaacaatca acgaaaagta
    30361 ggtaaaatat ggaaaaagta ttagtgatcg tcggtccgac agcagtcggt aaaacagcgc
    30421 taagtgtgga actcgcacaa ctatttggag gagagatcat cagtggtgat tcattgcaaa
    30481 tctataaaaa tctggatatc ggtacagcaa aaatcaaaga agaagagatg aataatgttc
    30541 cgcatcattt gattgatgtg atcgaaccta ctcaacgtta ctcagccgct gattttcagc
    30601 attcaggtcg acaattgatt accgagattg cgaaccgagg gcacctacct atcatagctg
    30661 gtggtacggg tctctatatc caatcccttc tatatgatta tcagttagga gcgacagaag
    30721 aagcgaatcc tgagattaga aaaaagtatg aaacgttagc tgaaacaatt ggttcccaag
    30781 cattatggaa tcagttgaaa gaaaaagacc cggcagctgc tgagaaaatc cactggaaca
    30841 atcaacgcaa agtgataaga gctctggaag ttttagagac gacaggacat agtatcacgg
    30901 cacctaaaga agagccaaag caattgtatg attacctcat gattggatta gatacgaagc
    30961 gtagtattct ttatgaacga atcaatcaaa gagtcgattt gatgctagca gaaggtttag
    31021 tagcagaagc gcgtaaggtg tatgagcttg gagaagtcca ggccagtcaa ggtattggat
    31081 ataaagaatt tttcccttat tttgaaggga aagaaacatt ggaagaagca acagaacaag
    31141 tcaaactaca ttcgagaaga tacgcgaaac gtcagttgac gtggtttcgt aaccgtttgg
    31201 atgcacagtg gtttgatctt ataagtgagc caaatcaaaa aagtcagatt gagcaaacaa
    31261 tcaaaaaatg gctggaggcg gaataaaatg gaagaaaaag tgatcatcgt aggggtagaa
    31321 accgaagcaa accagcgtta cttttcagag tcaatggaag agctagttca gctgacgaat
    31381 accgcatcag gagaagtcgt attcacaatt acacaaaaga gaccacaagt ggatcggcaa
    31441 acgattatcg gtaaagggaa attacaagaa ttagttcaac aagcggatgc gcatgaagcg
    31501 gatttgatca tctttaatca tgagctaact ccgagacaga gccaactgat taccgatgct
    31561 gttggtcttc cggtaattga tcgcgtgcag cttatcttag atatttttgc gatgcgtgct
    31621 cgttcgaaag aagggaagct gcaggttgaa cttgctcagt tagaatacct gctgcctcgt
    31681 ttagtagggc aagggaaaac attatctcgt cttggcggtg ggatcggaac aagagggcca
    31741 ggtgaaacaa agttagaaac agaccgccga catatacgga ataaaatttt tgctgttaag
    31801 aaagaattga aagaagtaga agctcatcga gagcgcaatc gccaaaaaag aaaaaatagc
    31861 gagatttttc agattggact tattggctac acgaatgccg ggaagtcaac tatcttgaat
    31921 ctattgacac aagcggatac ttattcgaaa gatcaattgt ttgcaacgtt agatccattg
    31981 actaagagat ggcgttttgc tgaaggattt gagattactg taacggatac cgtcgggttc
    32041 atccaagacc tgcctacgca actgatcgat gctttccatt ctacattaga agaaagtcaa
    32101 aatatggatc ttttgctaca tgtagtcgat gctagttctc cagatcgtat tttacaggag
    32161 cagactgtct taaaattgat ggatgaactg aatatgaaag aaatgccgat actgactgtt
    32221 tacaataagg cagaccagat cgacccagcg atgttcacgc ctacgctatt cccaaatgta
    32281 ttgatttcta ctcagacgaa agaaggtaag atggcgttga tcgaagcaat caaacgtcag
    32341 ctgatggaac taatgacccc ttatacactt tctgtgccaa gcaacgaagg acagaaatta
    32401 agcgagttga gaagacaaac aatggttttg gacgaagcgt acatggaaga aacaaacgaa
    32461 tatgaagtta aagggtttgc aaaaaccaat tcaaaatggt taagaaaaaa ccaataaaat
    32521 tttatttttt taa
//
LOCUS       DANKEF010000029        31896 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000029 DANKEF010000000
VERSION     DANKEF010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.029"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(26..601)
                     /locus_tag="PWZ48_001510"
     CDS             complement(26..601)
                     /locus_tag="PWZ48_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2261172.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(606..1580)
                     /locus_tag="PWZ48_001511"
     CDS             complement(606..1580)
                     /locus_tag="PWZ48_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HDL2261173.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1604..1648)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1863..2831
                     /locus_tag="PWZ48_001512"
     CDS             1863..2831
                     /locus_tag="PWZ48_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2261174.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2928..3221
                     /locus_tag="PWZ48_001513"
     CDS             2928..3221
                     /locus_tag="PWZ48_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDL2261175.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3339..3575
                     /gene="secG"
                     /locus_tag="PWZ48_001514"
     CDS             3339..3575
                     /gene="secG"
                     /locus_tag="PWZ48_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDL2261176.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3678..4433
                     /locus_tag="PWZ48_001515"
     CDS             3678..4433
                     /locus_tag="PWZ48_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2261177.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4436..6793
                     /gene="rnr"
                     /locus_tag="PWZ48_001516"
     CDS             4436..6793
                     /gene="rnr"
                     /locus_tag="PWZ48_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDL2261178.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6815..7279
                     /gene="smpB"
                     /locus_tag="PWZ48_001517"
     CDS             6815..7279
                     /gene="smpB"
                     /locus_tag="PWZ48_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDL2261179.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7581..8300
                     /gene="atpB"
                     /locus_tag="PWZ48_001518"
     CDS             7581..8300
                     /gene="atpB"
                     /locus_tag="PWZ48_001518"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDL2261180.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8353..8568
                     /gene="atpE"
                     /locus_tag="PWZ48_001519"
     CDS             8353..8568
                     /gene="atpE"
                     /locus_tag="PWZ48_001519"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HDL2261181.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8659..9183
                     /gene="atpF"
                     /locus_tag="PWZ48_001520"
     CDS             8659..9183
                     /gene="atpF"
                     /locus_tag="PWZ48_001520"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDL2261182.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9170..9712
                     /locus_tag="PWZ48_001521"
     CDS             9170..9712
                     /locus_tag="PWZ48_001521"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDL2261183.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9735..11291
                     /locus_tag="PWZ48_001522"
     CDS             9735..11291
                     /locus_tag="PWZ48_001522"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDL2261184.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11305..12207
                     /locus_tag="PWZ48_001523"
     CDS             11305..12207
                     /locus_tag="PWZ48_001523"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDL2261185.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12230..13636
                     /gene="atpD"
                     /locus_tag="PWZ48_001524"
     CDS             12230..13636
                     /gene="atpD"
                     /locus_tag="PWZ48_001524"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDL2261186.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13650..14072
                     /locus_tag="PWZ48_001525"
     CDS             13650..14072
                     /locus_tag="PWZ48_001525"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDL2261187.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14541..14771
                     /locus_tag="PWZ48_001526"
     CDS             14541..14771
                     /locus_tag="PWZ48_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HDL2261188.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14868..16169
                     /gene="murA"
                     /locus_tag="PWZ48_001527"
     CDS             14868..16169
                     /gene="murA"
                     /locus_tag="PWZ48_001527"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDL2261189.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16182..16358
                     /locus_tag="PWZ48_001528"
     CDS             16182..16358
                     /locus_tag="PWZ48_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDL2261190.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16493..16789
                     /gene="yidD"
                     /locus_tag="PWZ48_001529"
     CDS             16493..16789
                     /gene="yidD"
                     /locus_tag="PWZ48_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDL2261191.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16853..17320
                     /locus_tag="PWZ48_001530"
     CDS             16853..17320
                     /locus_tag="PWZ48_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2261192.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17405..18289
                     /locus_tag="PWZ48_001531"
     CDS             17405..18289
                     /locus_tag="PWZ48_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2261193.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18403..18639
                     /locus_tag="PWZ48_001532"
     CDS             18403..18639
                     /locus_tag="PWZ48_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HDL2261194.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18727..18807
                     /locus_tag="PWZ48_001533"
     tRNA            18727..18807
                     /locus_tag="PWZ48_001533"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18761..18763,aa:Tyr,seq:gta)
     gene            18814..18885
                     /locus_tag="PWZ48_001534"
     tRNA            18814..18885
                     /locus_tag="PWZ48_001534"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18846..18848,aa:Gln,seq:ttg)
     gene            19011..19181
                     /locus_tag="PWZ48_001535"
     CDS             19011..19181
                     /locus_tag="PWZ48_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261195.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19401..20108
                     /locus_tag="PWZ48_001536"
     CDS             19401..20108
                     /locus_tag="PWZ48_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2261196.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20167..20811)
                     /locus_tag="PWZ48_001537"
     CDS             complement(20167..20811)
                     /locus_tag="PWZ48_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HDL2261197.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20938..21225
                     /locus_tag="PWZ48_001538"
     CDS             20938..21225
                     /locus_tag="PWZ48_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261198.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21333..21653
                     /locus_tag="PWZ48_001539"
     CDS             21333..21653
                     /locus_tag="PWZ48_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2261199.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21971..23047
                     /locus_tag="PWZ48_001540"
     CDS             21971..23047
                     /locus_tag="PWZ48_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2261200.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23044..23763
                     /locus_tag="PWZ48_001541"
     CDS             23044..23763
                     /locus_tag="PWZ48_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HDL2261201.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23891..24442
                     /gene="raiA"
                     /locus_tag="PWZ48_001542"
     CDS             23891..24442
                     /gene="raiA"
                     /locus_tag="PWZ48_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDL2261202.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            24664..27198
                     /gene="secA"
                     /locus_tag="PWZ48_001543"
     CDS             24664..27198
                     /gene="secA"
                     /locus_tag="PWZ48_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDL2261203.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            27324..28425
                     /gene="prfB"
                     /locus_tag="PWZ48_001544"
     CDS             join(27324..27395,27397..28425)
                     /gene="prfB"
                     /locus_tag="PWZ48_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDL2261204.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            28530..29216
                     /gene="ftsE"
                     /locus_tag="PWZ48_001545"
     CDS             28530..29216
                     /gene="ftsE"
                     /locus_tag="PWZ48_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDL2261205.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            29200..30093
                     /locus_tag="PWZ48_001546"
     CDS             29200..30093
                     /locus_tag="PWZ48_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261206.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            30245..31693
                     /gene="cls"
                     /locus_tag="PWZ48_001547"
     CDS             30245..31693
                     /gene="cls"
                     /locus_tag="PWZ48_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDL2261207.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
BASE COUNT        10607 a         5565 c         6908 g         8816 t
ORIGIN      
        1 attttgctaa tccagctctg tttattcagc ctatttttct ttttgaatat atcaagcgtt
       61 gtggcaacat agtgatgact aatgaaacaa cgacgataga caacaaacga tcaaggtagt
      121 ctgtacctgc ctgtaccaag atcacacttg ttgtctgact taaacctaac ccatgtaata
      181 gctgaacgat catactggat cctgaggagg tgatcccgcc aaataactga acggtaatga
      241 tcgaagaaac gatcgttcca ggtaccgaca aaatcaaagc gagtaataac agtttcaagc
      301 tgtttttatc tttgccgaga tatcttggca aaatcaatcc agcaagcaag cctgtgagaa
      361 tttggattgg cgaataatac agagaaaaaa tatctgtagt cactccactt aaaataccgc
      421 tggacaatcc tgtcagcatt ccaggtacag gcccaagcaa tgcaccagca aaaatcgttc
      481 cgatagaatc gagatagatt ggcaaacgaa gccataaagc aatcgttcct ccaatataat
      541 tgatcccaat acacaaagcc atcactgtaa gtacgcgagt tgccattttg tttttattca
      601 ttttgttagc ccatctttaa cctttccaaa tctttcataa tcgtttcttc ttgtgcattt
      661 aaaagaacgg ttaaaaactt tcggaaaaaa agacgagtat ctacttttgt cataatttta
      721 ctgttagcag gtttctgcca aaattcataa cgatcaacca atgtttgtcc acgacttatt
      781 ccttgagttt ccacagcagt gaagcttttg aaaccttcac agatggcctc ctcgataaaa
      841 taagcaacag ctaacggatc attgatgaca catcctaaaa ttctttcttg tttccagtgg
      901 aagtcaaaat aaaaacgagt gatcgctttt agatactctc cctcttcagg attcatttga
      961 cagcagtatt cgaggatagt cggtgtcaag acgatttctc ttgtcacatc taacccaacc
     1021 atttctatcg tttgtttgag attctcgaat acataagaag ctgcttcagg atcacaccag
     1081 taattgtatt cagcaactgg cgaacagtta ccatggcttt tgtaagttcc acccatcgag
     1141 acaaaacgtt ccatatgttt cccaatattt gggttgactt tcaaagcaga agcgatattg
     1201 gtcaaaggac ccaaagcaat gactgatgta tttgtctttt ctttgaaata atcagccaaa
     1261 aaatcaacgg catggagagg ttctgcctgt tttctcaaga tcatcgggaa atttgtttcc
     1321 cctaaaccat ccataccatg agtgtcttgt gcactgatga aaggttgctt caaaggtttt
     1381 tccattcctt gataaacagg aatgtctaaa cgatcacagc gttccaggca tttcagcaca
     1441 ttctgcgtac ctatatctgc agggacattc ccacaaacta cggtaatagc aactacttcg
     1501 atatcagggg atttgacagc taatgataaa gctaaagtgt cgtcgatccc tggatcgcaa
     1561 tcaataatta cttttcgcat tatttccaat ctcctttctt tagagatact tagtttttta
     1621 ttctgggaag tttccgaacc agtccaagac gagcgtcttg attataatgc acgaaattag
     1681 tatactggct tttattgcga atgtaaatgc tctcttacaa gtaaatctat tgaggagaac
     1741 tttaagtaaa tgatacaatg aaaaagaaat ggaatttacg tgtgccgaaa ataaagaatc
     1801 ctatggatcg gtacattgga taatagatag attatagttt ggcagaaagc ggcgggtgta
     1861 caatggttat tttttatttc attcttattc tgataattat agggatcagt taccattttc
     1921 tttcttattt ttatgcaaag aaattagtaa gagcgggagt acagagagga tatgactggt
     1981 atgaagacac tggtcatcgt ttgatggacc ctgctgcagt cacgtcagta gaaaaaaaac
     2041 gacaaaaatt agaagaagag tgtgaaagct tttgggagca gggaacaccc aaaacaatca
     2101 aaagttatga cgggttgaaa ctagctggtc agatgttcat ccatccaaat aagcagaata
     2161 aatgggtcat ttgcgtacat gattaccgaa gcacagggaa aagagacatg tctcatatag
     2221 gtaaaagata tgcagagaaa ggattcaacg ttcttgtacc tgatttacgt gcgcacggag
     2281 aaagtgaagg agaaatcatc ggaatgggat ggctagatcg gctagattta attgcctgga
     2341 tccaactcat cttagatgaa cagccgaatg cgtccattat tttacacggc ggatcaatgg
     2401 gagcttctac gatcatgatg gcaagtggtg aaaaacttcc atcagcggtc aaaggattta
     2461 ttttagacag cggatatgtt tctgtttatg cagaattccg atatatgtta agtaaaatca
     2521 ctgtctttcc taaaaaaatg gtcatgagat atgcgaatca ttatgcccag aaatacgccg
     2581 gttactcctt aaagcaagca tcagctacga gacagcttgg cagcaatcat ctgcctttat
     2641 tagtcatcca tggtgaaaga gatcattttg tccctacaga agcagcgtac acgattcaaa
     2701 atgctacggc aggggataaa gcactgctgc ttgttccgga agcagaacac ttggaagcat
     2761 cgatgaaaga tccgaaaacc tattggacag tgattttttc atttattgaa caaagaataa
     2821 cgatgaatta attttctctt cttttttgta agtaattcga ggataaataa ttgtttatag
     2881 atcaggagac tgctaaaata aggtaggaat aaaaaaatat cagttatttg aggagtgcgg
     2941 catatggaaa tcaaagaaga aaaaatcgtc ttgctttatt caatgatgaa caacaagaga
     3001 tcggagaaat gacatggtca gatgcgggcc cagacatcat gatcgttgac catacatttg
     3061 tagatccagc atatcgtggt caaaagttag ctgaaaagct agtgtatact ggagtagaat
     3121 tagcaagacg agacgggaaa aagatcattc ctttatgccc ttatgcaaaa aaagagtttg
     3181 aaaagaaacc agaatatcaa gacgtcttac gtcagtcata agaataacag ctgaaagttt
     3241 tgaactttca gctgttattc catttatata gagttgtcac ggacttttat ctatgataaa
     3301 gttaaagaga cgatatcttt tgggaaggag gctgcgatat gtataattta attttgggaa
     3361 tcgtgatcgt tttatcgatt gttatggtca ttgcgattat gatgcagcca agcaagcaaa
     3421 atagtgcagc gagtgcattt accggcggtg cggatcaact attcggcaaa caaaaagctc
     3481 gcggctttga agctgtcatg cagcgatcaa ctgcagttat gggagctatc tggatgatct
     3541 tattatttgt cctagcatat ttatcttcga agtagttgaa taagcctctc gcaaaacggc
     3601 gggcggcttt ttttattgca aagagaacgc ttaagaagaa gttatgaaag aaactatggt
     3661 aaaatgaggc gaaagaggtg agacgagtga gacaattacc aaaacccctt tatgcaaaac
     3721 atggaaagcg agcggttttg ctgcttcacg cttactcagg aagtccaaac gatgttcgaa
     3781 tgctcgcacg atttttggaa aaagcagatt atacagttta tgcgccgctt tttaaaggac
     3841 acggaacaat ggagccatat gatatcttgc aagaaaaagc agaaagctgg tgggcagaca
     3901 ctaaaaaagc catccatttt ttacagtctg agcagttttc tgatattgcc gtattaggat
     3961 tatcaatggg cggcatcttt gctgtacgag cactagaaga agaatcagtg attggcggcg
     4021 gttttttctg ttcgcctttg tctcctgtaa aaacaaacgt gcctgaaaac tttgaaaaat
     4081 acgtacgtca agtgctgaag acagcaggta aatcagagaa agaaatcaat gaaaaagcag
     4141 ttgcttaccg ctcgttagct gaacaacaat taatggatat acaagatcag gcagcaatcg
     4201 tagaaagtag gttgtcagat atccaacagc cgatattttt agctcaagct gggaaagatg
     4261 aaatgatcga ccctaatggc gtatttgaaa ctgcacgcaa gttgagccgt cagcgagtga
     4321 cacttcaatg gtatcctgaa agtggtcatg tgattacagt tggtacagca agacgcgaat
     4381 tagaaaaaga cgtcttggaa tttttagaaa aattaccttg gaatgaggaa taaatatgac
     4441 aaaagaaacg ataaaagaga aaattctctt tttcatggaa agcagtcgca aacgcagttt
     4501 ttctatggaa gaaatcgctg aaggtttagg gttccaaaaa agcgatgatt ttaaattact
     4561 agtacaaacg attgctcaaa tggaaagaga acagagtata gttttcaata aaaaaggaaa
     4621 agtgaaactt cctttaaaac caatcctgat cgaaggaaca tttcgagcaa atgaacgagg
     4681 atttggattt gtaacgattg atcccgaaga agatgacatc tatatcccaa aagaagcgac
     4741 tggttttgca atggatggag acaccgtagc aatcgatatc atgaagcgag caaatacaga
     4801 tatggatcgc ggagcagagg ggaaagtagt cgagattcga gaccgggcga ctactcagtt
     4861 agcaggggaa ttcgttgctt attcagaaga agaaatacaa gaaacagatc tttacggcgt
     4921 tgttatcccg aaagatagaa agctcaacca atttaaagta tatgcagcag cagagggagt
     4981 tcgtccagtt gacggaagta ttgtgctgat cgaactgacc cattaccctg aaaaagggta
     5041 tgcgactagt ttagaaggga taatcaagca agtgattggt cataagaatg accctggcat
     5101 ggatatcctc tcaattgtcg tggctaatgg tatccctacg aaatttcctg atgatgtatt
     5161 gtctgaagca gatgaagttc cagatcaaat cagcgaaaag gatataactg gacgtagaga
     5221 tttaagaaat cagctcattg ttacgattga tggagaagat gcaaaagatc tagatgatgc
     5281 agttacggtt aaaaaattag ataacggtaa ttattttctt ggagtacaca ttgcagatgt
     5341 ttcttactat gtgacacaag gcagtcaatt ggacagagaa gcatatgaaa gagggacaag
     5401 tgtatatttg actgatcgag taattccgat gatcccgcaa cgtttatcga atggtatctg
     5461 ttcactgaat ccccatgttc caaggctgac catgagttgc gaaatggaga ttgatccaaa
     5521 tggacaagta gtctctcatg atattttccc aagtgtgatc caaacaacag aacggatgac
     5581 ctacacagct gtcaatcaaa tcttagaaga tcaagacgaa gaaacgatgc aacgctatgc
     5641 tcagctcgta cctatgttcc aagaaatggg tgaattgcac cgaatccttg aaacgatgcg
     5701 aatcaaacgt ggcgcgattt cttttgagga tcgcgaagcg aaaatccttg ttgatgctga
     5761 aggacaccca aatgacatcc agctaagaag cagaggaatt ggtgaacgct tgatcgaatc
     5821 tttcatgctt gcggccaatg aaacggtagc tgaacattat aataaattaa atctgccgtt
     5881 tatttaccga atccatgaac agccaaaaga agaaaaaatg cagcgcttct ttgattttgc
     5941 ttcagctttg ggcattctgg tccgaggaac gaaaggaacg atcacaccaa aagatttaca
     6001 gcatgtgatc gaaggagtag aagacaaacc agaagctgca gttatcaata ctatgttgct
     6061 aagaagcatg caacaagcac gctattcaga agacaatttt gggcattatg gcttagcggc
     6121 agattactac acacacttta catctcctat tcgacgctat cccgatctaa tcgtccatcg
     6181 tctgatccgt tcttacagtg aagataaaag tgaaagcaat caaagttcgt gggctgcgga
     6241 acttcctgaa attgcagatc atagttctaa gatggaacgt cgtgcagtag aaacagaacg
     6301 tgaagtagat tccatgaaga aagcagaatt catgatggac aaagtgggag aagaattcga
     6361 tgggatcatc agctctgttg tgaaattcgg cctgtttatc gaactgccga atacagtaga
     6421 gggattgatc catatcaatg aattgaaaca ggactacttc cattttgttg aaaatcattt
     6481 agctcttgta ggtgaaagaa ctggtctgac ttttaaaatc ggacagaaag tgcgagtaaa
     6541 agtagtcaaa gctgatccag atgaacgagc agtagatttt gaacttgttt ccgcagaaga
     6601 ggttacagcg ttggaacggc caaaaacacg aaatggaaaa tcaggaaaac gcgaaggaaa
     6661 acaagcagga aaacgcccag aaagaaatgg caagaagaac aaacgatata gcgatagtaa
     6721 gcaaacgttc ggtaagaaaa aagggaagaa gccattttac aaaggtgtta aaaagaagaa
     6781 aaagaaaaaa taaaatgagt tggaggaaac agctatgcca aaaggcgagg gaaaattaat
     6841 tgcacaaaac aagaaagctc gccatgatta ttcgatcatc gacacgatgg aagcagggat
     6901 ggtcttgcaa ggaaccgaga tcaagtcgat acgaaacagc cggatcaatc taaaagatgg
     6961 atttattcgc gtccgcaacg gagaagcttt cttgcataat gttcatatca gtccttatga
     7021 acaaggaaat atttttaatc atgatccgtt gcgcacgaga aagttattat tacacaaaaa
     7081 acaaatcatc cggcttgaaa atgaattgaa aaatactgga atcactgttg ttcctttaaa
     7141 agtctatatt cgtaacggct atgccaaggt attgattggt ctggcgaaag ggaaaaaatc
     7201 ttatgataaa cgggaagatt tgaaacgaaa agatatcgat cgacaaattg atcgaacatt
     7261 aaaaaatttc tctagataat ttcattatcc tttttcgcat attcggtgct tttgctttta
     7321 cttaatgata aaatttgtta gcatttaatc tgataaaaca tgattatcat aggttttttc
     7381 ttagatcagg agcaccgtgt ataaagaaaa attcgcttcg tttttgactg aaataaaaaa
     7441 gtgtctaatt ttcatgaaaa tgttttgaat tttctgtttc atggtggtat gataccgtgt
     7501 ggttagaaac cgattggaaa aaaccagata cgcacatgaa ttgattttat gtgaatttcg
     7561 gggaaaagag gtgaaatgga ttggatgaac gctcgctaac gttccacatt ggaccaattt
     7621 ggtttgatgg tacagtttgt ataatggtgt tactgacctg tttgattgtt ttcttcttag
     7681 cttacttttt tacaagaaat cttaagttga aaccaacagg caaacaaaat gcgatagaat
     7741 gggtcattga ttttagtcga ggaatcgtaa cagataatct gcctagaaaa gaattgagca
     7801 atttccattt gttggcattt acattgtttt tatttgtttt tgttgcaaac aacatcggtt
     7861 tagtaacaaa aatcgtttta cctaacgaaa caacactttg gaaaagcccg acagcagacc
     7921 cgtttgttac tttaactttg gcatttataa tgatcttact tactcactta ttcggggtga
     7981 aaaagttagg cttcaaagga tatttcgtaa attcgttctt gaaaccctat agttttatgt
     8041 ttccgatgaa actgattgaa gaatttacaa acttattaac acttgcttta cgtctttatg
     8101 ggaatattta tgcgggggaa gttttgctaa ccttgattgc aaaaatgatg acaagcttag
     8161 gctggttctc attaccatta gcaattccat tagagatggt ctggatcgcg ttctccttgt
     8221 ttattgggag catccaagca tttgtctttg tcactttgtc aatggtttat atgagccata
     8281 agattgaagt agaagaataa tactcggtta aaaaacaaac tactatttat ctattttagg
     8341 aggaaaacaa ttatgaatta tatcgcagca gcaatcgcaa tcatgggagc agcaatcgga
     8401 gccggttatg gtaacggtca agttatctct aaaacaatcg aatcaatggc tcgccaacct
     8461 gaaatgtctg gtcaattccg tacaacaatg tttatcgggg tagctctagt tgaagctgtt
     8521 cctatcttag gggtagttat cgccttgatt ctagtttttg ccgtttaatt gaatcagtat
     8581 taaggcagaa acaacatcgt ttgttactgc ttttttacat ggaaactaga aatactccaa
     8641 agaaaggaag gctcgcctat gctgaatcaa ttggcaattg cagaagttgg caatgcgatg
     8701 ttaggtaata tcatcgttgt cagcggctca tttttgatac tactggctct cttgaagcac
     8761 tttgcttggg gaccaatcag cgatattttg aaaaaacgtg aagacaagat cgccaatgat
     8821 ttagattctg cagaacaatc tcgcatcaac tcagcgaaaa tggaacaaga acgcgaacaa
     8881 caattgttag cctctcgttc tgatgcagct gatatcatca aaaatgcgaa agaaagtgga
     8941 gaattaagcc gccaaaatat tttgaaggat gctcaagaag aagcagctcg tctaaaaagc
     9001 aaagcccaag ctgatatcac tgtagaacgt gattcagcgc tgaactctgt aaaagacgac
     9061 gttgcagaac tctctcttca aatcgcggaa aaaatcttga acaaagaatt atctccagaa
     9121 atgcatgaat cattaatcaa tcaatacatt gaaggtctag gttcttcaaa tgaaactaga
     9181 taaatataca gtaggaagac gttacgggaa agctttattt gaactggcga tcgattcaaa
     9241 tcaagcagaa gaaatctatc aagatctcgt aagtttacgt caaatctatg aacaagtacc
     9301 tggattaggg aatatgttaa gtgatgttcg tctagaacct catgaaaaac gagaaatcat
     9361 ggacaagctt gtcagcggtt atgatgggat cgttaaaaac tttttagaag ttgtttatga
     9421 gtataaccga atggatgacc ttttgttcat gatcgaagaa tatgaacgtc gatatgatga
     9481 acatcaaggg ttactattgg gaagtgtcac aacagcagtc ccactttcag acgaacaacg
     9541 aatacaatta gaaaagaatg ttgcaaaaac aatggactat caatcagttg aattaaaaca
     9601 aatcgttgat tcttctatta ttggtggcgc gattgttgaa gccaaccatc gagtaatcga
     9661 cggaagcatt cgcacgcaat tagaaaaaat gcgtaatcaa ttaaacagat agtacaaaga
     9721 ggtaggtgaa tcgaatggcc atcaaagcag aagaaattag tgccttgatt aaagaacaaa
     9781 ttgaaaatta tcaaaacgtg ctttcagtcg aagaaatcgg taccgtaact tatgtaggtg
     9841 acgggatcgc tcgtgcacat ggtttagaaa acgcgatgag tggtgagttg cttgaatttt
     9901 caaacggctc atacggaatg gcgcaaaact tagagtcaaa tgatgtaggt atcattatcc
     9961 ttggcgattt cgagtccatt cgtgaaggag ataaagtaaa acgaacaggt aagatcatgg
    10021 aagttccagt tggagaggcc ttgatcggtc gggtagtcaa tccgctaggt caaccaatcg
    10081 acggactagg tgaaatcgtt actgacaaag ctcgtccagt agaagcgatg gcaccaggtg
    10141 ttatgcaacg taaatctgtt aacgaaccaa tgcaaacagg gctaaaagcg atcgacgccc
    10201 ttgtgccaat cggacgcgga caacgtgaat tagtcatcgg tgaccgtaaa acagggaaaa
    10261 cttctattgc gatcgatacg atcatcaacc aaaaaggtca agatatgatc tgtatctatg
    10321 tagcaatcgg acaaaaagat tctacagttc gtacacaagt tgaaacattg aaaaaatatg
    10381 gcgcaatgga ttacacaatc gttgtgaatg ccggtgcgtc tcaaccagca ccattgcttt
    10441 atatcgcacc atatgctggt actgcaatgg gtgaagaatt catgtacaac ggtaaacatg
    10501 tattgatcat ttttgatgat ctttcaaaac aagctgttgc ttatcgtgaa ctttccttgc
    10561 tacttcgccg tccaccaggt cgtgaagctt atcctgggga tgttttctac ttgcactcac
    10621 gtttattaga acgtgcagca aaattaagtg atgatttagg tggcggttca atgactgcct
    10681 taccatttgt tgaaacacaa gctggagata tctctgctta tatcccaaca aacgttattt
    10741 cgatcactga tggacaaatc ttcttagaaa gcgacttgtt ctacgcaggt actcgtccag
    10801 cggttgatgc cggactatct gtatctcgtg taggaggttc tgcgcaaatc aaagcaatga
    10861 aaaaagttgc tgggacttta cgcctagact tagcaagtta ccgtgaatta gaagcattta
    10921 cacaattcgg ttctgacttg gatgcagcaa cacaagcaaa actaaatcgt ggacgccgta
    10981 cagtagaaat cttgaaacaa aaattgcatg caccgctagc tgttgaaaaa caagtagtga
    11041 ttttgtatgc tttgacacat ggattcttag atagtatccc tgtagacagt attttagact
    11101 tcgaacatga attattcgaa tatctggata ccaatcattc agatatcttt gaaacaatcc
    11161 gtacaacaaa agatcttcca gaagaagaag cgttgaacag tgcgattcag gaatataaag
    11221 acatgttctt agctacaaaa ggaagtaatt cctctacaga agataaattg aaatccattc
    11281 aaaatgcata gtgaggtgag atgaatgggt gcttcattaa atgaaatcaa gacacgtatc
    11341 gcttcaacga aaaaaacgag ccaaatcacg cgggcaatgc agatggtgtc tgcttctaag
    11401 ttgacaaaat ctgaagcttc ttcacagaag tttcaggttt atgcaacaaa agtaagagaa
    11461 atcgttacgc atttgactgc tactcagtta aatgatattg cctcagaaaa tcctagaggg
    11521 gatatcaatt acaacagcat gctgctcagc cgtcctgtga agaaaactgg ttatatcgtc
    11581 atcactgcag atggcggttt agtcggtgga tataacagct cgatattgaa acaaacgatg
    11641 tctattttag aagaagacca ccaatcaccc gatgattacg tgatgatcgc aattggcgga
    11701 actggtgcag acttctttaa agcacgcggt atcaatctag catatgagct gcgcaatcta
    11761 tctgatcagc caagctttga tgaagtccgc aaaattgtaa atatggcaac aactatgttc
    11821 caaaacgaag tattcgatga attatacgta tgttataacc atcatatcaa ttcgctaact
    11881 agtcagtttc gtgtagaaaa aatgctgcca atctcagact tagatccaga agaagcaacg
    11941 actttcgaac aagaatatat ttttgaacct tcaaaagaag aaatcttgtc tcagttattg
    12001 cctcagtatg ctgaaagttt gatctacggt gcgatcgttg acgccaaaac agcagaacat
    12061 gctgctggta tgacagcaat gaaaacagca actgataatg ctgctaatat cattgatgag
    12121 ctgacggttt cttataaccg tgcaagacaa ggggctatta cccaagaaat taccgaaatt
    12181 gttgccggag cttcggcact agaatagtat tgagattgga ggaaaaaaga tgagttcagg
    12241 caagattgtt caagtaatcg gtcccgttgt cgacgtggaa ttttctttag accaatcctt
    12301 accagatatc aacaacgcat tagttgttta taaaaatgac gaaaataaat caaaagttgt
    12361 tcttgaagca gcgttagaat taggggacgg agtgatccgg accattgcga tggaatcaac
    12421 agatggttta caaagaggaa tggaagtcat tgatacaggc aaagcaatct ctgttcctgt
    12481 aggtaaagaa acattaggtc gcgtattcaa cgtactagga gatacgatcg atttagaaac
    12541 acctttccca gaagatgcgg aaagaagcga aattcataaa aaagcaccag cctttgacga
    12601 attaagtaca agtacagaaa ttttggaaac agggatcaaa gttatcgatt tgcttgcccc
    12661 atatttaaaa ggtgggaaag tcggactatt cggtggtgcc ggtgttggta aaaccgtact
    12721 gatccaagaa ctgatccata atatcgccca agaacatggt ggtatttctg tatttaccgg
    12781 tgtaggtgaa cgtactcgtg aaggtaatga cttgtattat gaaatgaaag attcaggagt
    12841 tatcgaaaaa acagccatgg tgttcggaca aatgaacgaa ccaccaggtg cacgtatgcg
    12901 tgttgctttg actggtttga cgattgcgga atatttccgt gatgtagaag gtcaagatgt
    12961 actgttgttt atcgacaaca ttttccgttt cactcaagct ggatctgaag tatcagcctt
    13021 gttaggacgg atgccttctg cggttggtta tcaaccaaca ttggcaacag aaatgggtca
    13081 attgcaagaa cgtatcacat ctacgaaaaa aggttcgatc acatcgatcc aagcaatcta
    13141 tgtgccagcc gatgactaca ctgacccggc gccggcaaca gcattcgccc atttggatgc
    13201 gacaaccaac ttggaacgta aattaacaga acaagggatt tatccagcgg tagatccatt
    13261 ggcttcttct tcaagtgctt tagcacctga aatcgtggga gaagaacatt ataaagtagc
    13321 aacagaagtc caacacgtat tgcaacgata tcgtgaatta caagatatca ttgctatctt
    13381 aggtatggat gaattatccg atcaagaaaa agtgttagtt tctcgtgccc gcagagtcca
    13441 attcttcttg tcacaaaact ttaacgtggc tgaacaattt actggtcttc caggttcata
    13501 tgtccctgta gaagaaacag tgaaaggatt ccgcgaaatc ttggaaggca aatacgatga
    13561 tcttcctgaa gaagctttcc gaagtgttgg ccgaatcgaa gacgtcgtag aaaaagcgaa
    13621 aacactaggc tactagaaag gggtgcccca tggatcaata tttaacagta aatgtggtaa
    13681 ctcctgccgg tttggtctat gatcaccatg cagctatcgt cgttgcacgt acgacagctg
    13741 gtgagcttgg gattttgcct aaacatgcac caatcatcgt tccattgacg attgatgaag
    13801 ttcgtgtaaa acgaacagat tcagataccc acgtcgattg gatcgctgtt aatggcggaa
    13861 tcatggaagt tcgtgataat gttgtttcaa tcgttgctga tagtgcggaa cgtgaacgtg
    13921 atatcgatgt atctcgtgcg gaacgtgcaa aacaacgcgc agaacgccaa atcgaagaag
    13981 caaaagcaaa acaagatacg aacgaattga aacgggcaac tgttgcgttg caccgagcaa
    14041 tcaatcgtat caaagtatct aagcactcat aaaatgagat gcggatgttt atccgtgttt
    14101 aagaacagta aaaagaggtt atgacagaag tggtcagctt caagacataa gaaggaattt
    14161 acgaaaattg cttctcaaat ttttgtgaaa tctcagctta tttttcgaag aagctacttc
    14221 tggaacaccg tatatcagag gttgtgaaat cagcgttttg tcctgagtat tagagaaaca
    14281 aatggaaaat agttcctcat attttgccgt tttttgagct aataccgagg gtcagatgat
    14341 tgaacaccgt ttatttggaa taagggactg cgacaagact tttgtctcag tccctttttt
    14401 tgcatatttt tagcatttat ttcctaaaaa atgtaagggg attcctatat ttttaagtat
    14461 acttgttttt ccttttatac attttatggt attatcattt tattagttat tataaaacaa
    14521 tcgttagaaa ggacgtcatt atgcaagtat acggaattga tgcggtgctt cgaatcgtga
    14581 gtcatttggc gtttatctac ttagccttct ggtctttacg ttcgttacgc attgaaactt
    14641 ttttcagacc aatgcataat acgcaaatta gaatggcaat cgtacttttt tcaatttttt
    14701 tagggtacac tgcaagtaat tttttcttag aggttatcgc tttatgccga aatcttttcg
    14761 ttagcctgta aattttaaaa aaaggtacta ttttaaaagc aaggatgtta taatagaaaa
    14821 gattttgagt aagtgtatgc tcattggaat tttttggagg gaacaacatg gaagagatca
    14881 tcgtaagagg tggcaatcaa ttaaatggaa cagtaagaat tgaaggagct aaaaatgctg
    14941 ttttgcctat tttagctgct agtttgttag cagaagaagg tattacgaca ttagacaacg
    15001 ttccgattct ttctgatgtc ttcactatga atcaagtgat tcgtcactta aacgtagatg
    15061 tagattttga tgaacaaaag aatcaagtaa caatcgacgc ttcaagacaa ttagaaatcg
    15121 aagcgcctta tgagtatgtt agtcaaatgc gtgcttcaat cgttgtgatg ggtccgctac
    15181 ttgccagaaa tggacatgca aaagtagcta tgcctggtgg atgtgcgatt ggtaaacgac
    15241 caatcgattt gcatttaaaa ggatttcaag ctttaggagc aaaaattatt caaaaaaacg
    15301 gctatatcga agcaattgcg gatgaattga tcggtaacac gatttattta gatttcccta
    15361 gtgtaggtgc tactcaaaat atcatgatgg ctgccgtaaa agcaaaagga acaaccatca
    15421 ttgaaaacgt agctcgcgaa ccagaaatcg ttgatttagc aaacattttg aataaaatgg
    15481 gcgctcaagt ctatggagct ggtactgaaa caatgcgtat tgaaggcgtg gatcatttac
    15541 atgcagtcaa ccactcaatc gtacaagatc gtatcgaagc aggtaccttt atggttgctg
    15601 ctgcaatgac acaaggcaat gtgctgattg cagatgctat ctctgaacat aaccgcccat
    15661 tgatttctaa attgatcgaa atgggtgcag aaatcattga agaagaaggc ggagtccgtg
    15721 tcatcggacc aaaacatatc ttacctacag atgtaaaaac gatgcctcat cctggtttcc
    15781 ctacagatat gcaagctcaa atgacagcta tccaattagt tgcagaaggt acaagtgtgg
    15841 tgactgaaac agtgtttgaa aatcgtttcc aacatttaga agagatgcgc cgtatgaacg
    15901 ctcatgtcaa aatcgacgga aatgtagcaa tcatggatgg aaaccatgaa ttgcaaggtg
    15961 cagaagtata tgcaacagac ttgcgggcag cagcagcatt agttttagct ggcttgaaag
    16021 caaatggaat cacacgtgtg cgtaacttga attatctaga ccgtggatat tataatttcc
    16081 atatcaaact acagcaatta ggtgcagacg tcgaacgtgt agacatggat caaacttctg
    16141 ctgaaaaaac agcacaaacg attgcttaag gaagtggctg aatgagtaac caacgatata
    16201 tcttattaac tttgataaag attttagtgg tgatcttatt gcttatcctt ctttttgtgg
    16261 ccggaacgat gatcggatat ggtgtcatcg gtggcggcaa tccatttaaa gtattccagc
    16321 caagtttgtg gattcatatt cgagactttt ttcattgata aataagaatc acagtggttc
    16381 tagtcgatat gaagtgagcc atgaagcaga acatgataaa agaaaacaga agggaacgtt
    16441 gacgttagtc gcgatccctt tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa
    16501 gatattgatt tatttagtta gaggatatca gcgctttatt tcgcctgcat ttccacctag
    16561 ctgccgttat tacccaacct gttccaatta tatgataaaa gcaattcaaa cccatggtgc
    16621 gctaaaaggg acggtaatgg ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg
    16681 cggtatcgat tatgtaccat tgacattccg attaacgcga aataagaaag ataaaaaaga
    16741 accagattat aaaaaggttg tgaaaaatac gggtagcact gagcgataaa gaaaaagagg
    16801 aaatttattg tcaaagtaac aactccgcaa ctacttatat gggggacaaa aaatgatcga
    16861 agaactaaac aagaaagatt taccatggtc cttgttatta gatgcagatc ctgataaaga
    16921 aaaagttcaa gtatatgttt ccagagggag tggctggata tggaaagaaa aagaaaaaac
    16981 aataggtgta ctgatctatg tggcaaggga aaaagaatac gaaatcgtca atgtagcagt
    17041 aaccccaacg tatcaaggaa aggggattgg cggaaaatta cttgagacag cttttcagaa
    17101 gctttcacag ctagcttcaa accaaactcg gatcattata cgtactggca ctactagtag
    17161 cgctgcgtta cacctctatc aaaaaatggg ctatgtggag attggtcgag tcaaagacta
    17221 ttttatccat cattactctg agcccatttt tgaaaatgga gatcaattaa gggatcaagt
    17281 gattttagca attcacttac ctaaaaatag ctttaactaa taaactttca attcgagaaa
    17341 atgctttcaa atgctaaata gatacattac aatagcttta gcaaacaaaa aggagaatat
    17401 ttttatggga atattagttt ttgattttgg tggatcggct gtcaaatatg gttgttggga
    17461 tggcaaagag attaaaggta agggacaatt tgcaacgcct gaatcttggg aagaaatgaa
    17521 aacgcaatta tttcaagttt ataaaaaaat actcgtttct tttgaagtcg aaggaattgg
    17581 aatcagctct cctggagtag tgaataatga gaaacaggtg attgagggaa tcagtgcgat
    17641 tccttatatt catggattta atattttccg agatttggaa actctgtttc aactcccagt
    17701 aacgattgaa aatgacgcca actgtgccgg aatggctgaa ttttatgaag gtgctgcccg
    17761 tgattatcaa gatgttgctt ttgttgtggc aggaactggg attggcgggg cgttgttcac
    17821 gaaaggacaa ataaataaag gggcacatct ttacggtgga gaatttgggt tgatgttctt
    17881 agatggcgat aagacattca gtaaacttgg tacagcggta cagatggctt ggcgttattg
    17941 tgaacgcaaa ggtctcgata aaaatgctta taccggaaaa gatgtctttg aattagcaga
    18001 aactggagat gcaatagcaa aagaagaagt tgagagtttt tatacgtatc ttacaaaggg
    18061 attattcagt attcaatttt cgtttgatcc agaagtgatc gtattaggcg gcggggtttc
    18121 tgcaaaagaa gggctcattg atgagatcaa aagccgaatg aagaagctga cagaacaatt
    18181 cgatctgcat gatttcgaac cgcagatttt attatgtgag tacagaaacg atgctaatct
    18241 tataggtgca gcagcaaact atctcgctgt acgtcaagga gagaattaag agctttagag
    18301 aaggtgcagc ttgcataaaa gaagagaatg aaatgaatag gagattttta cctaaggaat
    18361 gttttatttg ctgcagtatg ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat
    18421 aaagatattg aagtgcgtat agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa
    18481 gtcactcagt tatttatagg gaaaaaaatg attggtgaga tcttagccta tgggccaaaa
    18541 gaatatgaaa ttttctttgg tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg
    18601 atagaaacag tgattcgaca ctggaatctg cacgaataaa aaaatatcaa aaaaactttc
    18661 aataaagggt tgcaaaaatc tggaaaatta ggtattataa ctaagtcagg ttgatacaaa
    18721 cctttcggag gagtagcgaa gtggctaaac gcgacggact gtaaatccgt tccttagggt
    18781 tcagtggttc gaatccactc tcctccattt cattggggta tagccaagcg gtaaggcaag
    18841 ggactttgac tccctcatgc gttggttcga atccagctac cccagtatac cacaatcgta
    18901 agctctcgtt agttcgagag ctcttttttt ttgctttgct accttgttcc ttgaagattc
    18961 tgctgaatta agatagagtt aagttaacta caagaattgg agggatccac atgaaatgga
    19021 aagaaaaagt agacgaagca gctgaaaagt tatatgattt ggtcaaatca gaaaaataca
    19081 atattgaagt gaatattccg aaaaaaggcg gaaaagcggt ccaggtaaaa tcaaaacgcc
    19141 caacgaatca tacaaaaaaa tggatggcta aaaaccgcta ataataacaa aagatccata
    19201 agtcggcatt ttcatcgatt ttagggtctt ttgttatttt ctaggttgaa catatctaaa
    19261 aaattgtttc tgacaatagg agtagggaga acgatgtgtg tctaaatcct ctgttgatgg
    19321 atagaaacct ttatcttttt cagttgtgaa tcaggtatat accagttata atagaagaaa
    19381 cgaaaggacg ggtgtacgtt atgtcgaatc aattacctgt atatatacag attcatgacc
    19441 aattaaaaaa tgaaatcgaa caaggtatat ggaaagtcgg agaccgtctt ccttcagaaa
    19501 gagaattagc agtgaaattc aatgtcagcc gtatgacgtt gagacaagca attcaaaact
    19561 tggcagatga aggaatctta gaaagaaaga tcggttcggg tacgtatgtt gcgcgagaaa
    19621 aagtacaaga aaaaatgtct ggtgcaacaa gttttacaga aattatggaa tcacaaaatc
    19681 gagtaccgtc tagtcggaca atctcttatt tcttgacttc gccaagctca agtgaaatgg
    19741 aaaaactaaa cttgagtaaa gatgacacga tcttgcgaat ggaacgtatt cgtttcgcag
    19801 atgatgtccc tatttgtttt gaagttgcca gtgttccaca aaagctgatc gatggctaca
    19861 gcaaagcaga gattactgaa tcgttgtatc gcacgctaga agaaaaaggc gggaaaaaga
    19921 tcggtccagc gaatcaaacg attactgctg tgttggcttc tgagaaaatt gctgactatt
    19981 tagaggtaaa acgaagagat gcagttcttc gtttgcgcca gatttcttat tttgaagacg
    20041 gtacgccgtt tgagtatgtg cgtacgcaat acgtaggaaa tcgttttgag ttttatttgg
    20101 aaaaataaaa aacaggtgat ccctgattcc ttattagaaa taaaagagaa tcagaatcac
    20161 ctgtttttat atatgatcag ttatcagttg ctcgtgataa gaaacagaac cctcttcaaa
    20221 ttggacttgt ccgttcagca gctcaatgat ggattcttta aagtggttag cacttttttc
    20281 atctacagca cagatgacgc taacttgatc agtatattgg atttctttta taaaaaaatg
    20341 ttggagttca agataattct gtaattttcc tagttgcgga tacgtgatca tcacagagat
    20401 ctcctgttgc aaggtacctt ctacgattcc aatgtcagac aatgcttgag agaccgcatg
    20461 agtgtatgct cgtattaatc cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac
    20521 tactgcagct acattgatca gttgattttt tttgaggact tccaacatcg gtacgcccgc
    20581 cgttccactt ggttctccat catcgctgct tcgctggatt tcattttttt ctccaatgat
    20641 gaaggcgctg cagttatggt tcgctttcca atgttctttt tttatttctt gaatgaaatt
    20701 ttttgcttgg tcctctgatt ctattcgtct aagagaacag ataaagcgtg attttttgat
    20761 ctcaatctca ctttgtccat tttcttttat tgtacgatag cgctcgatca tcttgtcacc
    20821 tctttttttc attttcttta ttcagtataa ataaagttct aggttgtttc aacactgtaa
    20881 cggttgcgca attgctgtag tatgcgataa taaagacaaa ggcaggtgga tctggggatg
    20941 caggaacgaa tgaaaaagta cgatgcaatt acgcattatt tgaagaacaa tggcggctct
    21001 caagtaacgt tgacctttac ccaatttgac gaactgctgt ttcccagtaa cggacttccc
    21061 aaaacagcaa gggaaagtac agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt
    21121 gcctatggct ggataaatgc aggctatgaa gtagttgtca tcaatttgga caaagaatat
    21181 gtcgtgttca ataaattagt gaaatctagt tggctatttg attaaataaa ccgcgtttag
    21241 aaaacgttga caaatttctt tctttttata taataaagta acagacgcga agaaaacgtt
    21301 tgcgtagttt gttttaaaaa ggagagaaaa atatggctaa aaaaacaatc atgcttgtat
    21361 gttcggctgg tatgagcaca agtttactag taacaaaaat gcaaaaagct gctgaagcaa
    21421 aaggaatcga ctctgatatt ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa
    21481 ataaagacgt ggatgtgttg ttattaggcc cacaagttcg ctttatgaaa gctgattttg
    21541 agaaacgtct ggaaccaaaa ggcattcctt tagatgtcat caatatggct gattatggta
    21601 tgatgaatgg cgaaaaagtg ttagatcaag caatcgcgct cataggtgaa taaatagaac
    21661 agtataacta tcgaacaagc gatccaaaaa atagatcgct tgttttttcg tatatttttt
    21721 tagggcgaat aatatgaatc atttttgggg tagaaggctt ctgacgagag atattaaagc
    21781 aatagactgt gaggaggtaa atttaccaaa ttatgagaag atcgcagcag tggaacgttt
    21841 gggcaactgg attctttgtc atcgctgtgg aatgaggacg tccgtcattg ttggacagct
    21901 tgcagattac ggttattttt gtacacattg tctatattta gggtgatgcg acactcaaca
    21961 agatttatat ttgtttgatc agcctgaagc aaaacctcga gaagtcgtct ttttttggac
    22021 aggacagctt actgaaaaac aaacggaaat cgcaaagaga atactttgcc attcagaaaa
    22081 aagacatcat cttatttggg ctgtaacagg tgcagggaaa acagagatgc tttatcctat
    22141 tttagtggaa actttaaaag ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt
    22201 ttgtaatgaa ttgtttttac gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct
    22261 ccatgggaat acagcaacgg cttatacctt tacttctttt gttatctgta cgattcatca
    22321 gctataccgt ttttatcggt cctttgattt aatagtgatc gatgagatcg atgcgtttcc
    22381 ttataacggg gatgctgggc tagctcacgc tgtgcaaaca gcaatcagat caaacggccg
    22441 attcatctac ttaagtgcca cgcctgatga gaagttacta aaggaaatca agcagacatt
    22501 tgagctccac aaacttgcct tgcgctttca tcggcgcttg cttcctgaac ccgtgcttgt
    22561 tttcatgaat aattggcgac aaaaatgtgg aaacaaaaag aaaatcaact ctttggtgcg
    22621 gatcattcag caactaatca aagaaaaccg tatcctttta ttttgtccaa gtattacgat
    22681 gatgcatcgt ttaaaaatca cactgcaaga agcttttcca gaatatgaga taacagatgt
    22741 ttcttcacaa gacgatcaac gggcaaagaa agttcagaaa atgcgagaag caatatacga
    22801 gatactgtta accacaatga tcctagaaag aggagtcaca tttgaaagaa tctctgtcat
    22861 cgttttagga gcagatcatc cagtttttac taaatcctct ttggtgcaaa tcgcaggaag
    22921 agctgatcgt aaaggtcctt atacgaacag tcgagtctat tttttctatg aagaaaaaac
    22981 ttcagctatt cgaaaagcct gtcaggaaat caaagaaatg aacgaagaag ggaagcagat
    23041 gctgtgagat gtagttgctg cggtaaagac atcattcaaa atttaacatt cagcgagatc
    23101 atcggagtgt cgaaatctag acttcctcag acatgttcag tttgccgcgc acaattccat
    23161 ttattagaag caaaaggttg tgcaggttgt caaaaaccaa tccatttgaa atacgaggag
    23221 acgaaaaaga aagcaaatac tagtttcgtt tactgcaacg actgtcaaga atggaagaag
    23281 aaatatcctg catatgattt tcaacaccgc gcctttttta cgtacgatga aaagatgcag
    23341 gcttggatca atcggtacaa gtttatggga gatatccgat tagcagggac ttttgcatat
    23401 aaatggactc agataaaaaa agattacccg gcatatattt attgtccaat acctttatct
    23461 gaaaaaaggt ggctagaaag aggattcaat caagtaagcg agatgttgaa agcagcaaaa
    23521 attccttttc agatgctgct caaaagagag caacatctgc ttgctcaagc aaaaaacacg
    23581 cgaaaggaaa gattggagat gcctcagcct tttgagcttg ccgtacctct ttctaagata
    23641 caacataaaa aaatattgat tattgacgat gtctatacaa caggacagac gctttttcat
    23701 gcagcagatt gcttgcagaa cgcacagccg aaaatcatcc gaaccatttc tttggcaaga
    23761 tgaatcagat gtggaaattt gatagcgatt taatttgcaa ataacctaga gttcgatata
    23821 atagaattaa gaagagggaa agagtggtcc ttctccttct tgattgtggt agacgaaagg
    23881 ggtaattttt atgtttagat ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat
    23941 tcgcgactac gttgagaaaa aagtaggcaa actagaacgc tatttcagtg acgcacccga
    24001 tgcaacggcg catgtgaact taaaagttta tacagaaaaa acagcaaaag tggaagtaac
    24061 gatcccatta ccttatcttg tattacgtgc cgaagaaact tctccagatt tatacgcaag
    24121 tatcgatttg gttgttgaca aattggaacg ccaaatccgc aaattcaaaa caaaaattaa
    24181 tcgtaaatcc cgtgaaacgg gcatgaatac tgcagatgct gctgctttat tcaatgaaga
    24241 ggacgaaaac gaaaatgatt ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa
    24301 accaatggat agtgaagaag cagtcttaca aatgaacatg ctgggacata acttctttat
    24361 ttttgaagat gcagaaacaa atggtacaag tatcgtttac cgccgtaaag atggaaaata
    24421 cggactgatt gaaacagact gatcagcctt acttaaataa caacgaaaac ttgcagcagc
    24481 tactgacagc tgccgcaagt ttttttgagt ttcctcctct tttagatgaa atatgaataa
    24541 attatgaagt tcttacttta atacgcttaa gatatttcaa ttatctataa ctaatgataa
    24601 aatgaaaagt aaagtctaca tagtctggat aatacacaga aggaaaagga gattacacaa
    24661 cgcatggcca atttgattcg ttcacttatc gaaaacgata agaaagaact aaaacgttta
    24721 gatagcattg caaaaaaagt tgaagcatac gctgaccaaa tggcagcatt aactgatgaa
    24781 caattacagg caaaaactgc tgaattcaag ggcagatacc aaaaaggaga aacattagat
    24841 caattacttc cggaagcttt tgcagtcgtc cgagaagcag caaaacgtgt attaggttta
    24901 tatccatacc acgtacaatt gatgggtgga atcgtgcttc acgatggaaa tatccctgaa
    24961 atgcgtactg gtgaaggtaa aaccttgacc gcaacgatgc cagtatatct aaatgctctg
    25021 actggcgaag gggtacacgt agttaccgtc aatgaatatt tagcaacccg tgactcgacg
    25081 gaaatgggcg aactttataa tttcttagga ttaacagtgg ggttgaatat caactcaaaa
    25141 tcttcggatg aaaaacgcga ggcttataat tgtgatatca cttatagtac gaataatgaa
    25201 ttaggatttg actacttgag agacaatatg gtcgtttacc gccatcaaat ggtacaacgt
    25261 ccgctgaatt atgccattgt cgatgaagtt gactcaatct tgattgatga agcgagaacg
    25321 ccgctgatca tttcagggca agcagaaaaa tccacagctt tatatacaag agcagacaac
    25381 ttcgtgaaac gtttgaaaga agaagaagat tataaaatcg atgtacaatc aaaaacaatc
    25441 agtttaaccg aagcagggat cgaaaaagca gaagaaacat tcggtttgga taacttatac
    25501 gacattgaaa atactgcatt gacccatcac ttggatcaag cattgagagc caactatatc
    25561 atgcttttag atatcgatta tgttgtccaa gacggaaaag tactgattgt tgaccagttc
    25621 actggtcgga tcatggatgg acgccgttat tcagatggac ttcaccaagc aatcgaagct
    25681 aaagaaggcg tagagatcga agacgaaacg aaaaccatgg cgacgatcac tttccagaac
    25741 tatttccgta tgtacaagaa acttgcggga atgactggta cagctaaaac agaagaagaa
    25801 gaattcagag aaatttataa catccaagta taccagattc cgacaaatcg tccggttatc
    25861 cgtgatgatc gtccagattt gctttaccca acattacaaa gcaagttcca cgctgtcgtg
    25921 caagatatca aagatcgcta ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt
    25981 gaaacatctg agttattatc agatatgctg aatcaagaac gtattcctca tgaagtgctg
    26041 aatgcgaaaa atcactttaa agaagcagag atcatcatga atgctggtca aaaaggtgct
    26101 gtgacgattg ctaccaacat ggctggtcgg ggaacagata tcaagttagg acttggggta
    26161 agagaacttg gtggtctagc agttatcggg acagaacgac atgaatctcg tcgtatcgat
    26221 aaccagcttc gaggacgtgc aggacgtcaa ggagaccctg gtatgtcaca gttctacttg
    26281 tcattggaag atgatttgat gaaacgattc ggttcagaac gaatcaaagc tttcttagat
    26341 cggatgaaga tcggtgagga agatgctgtt atccaaagca agatgctgac gaaacaagta
    26401 gaatctgcac aaaaacgtgt agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa
    26461 tacgatgatg tgatgcgtga acaacgtgaa gtaatctata aacagcgaca agaagtaatc
    26521 atggaagaaa aaagcttatc caagccacta atgaacatgg tcaaacggac aatcagtcgt
    26581 gtagtagatg cgcatacaca attagagaaa gaaaattgga atttggaaag tatcatcgat
    26641 tttgccggca gtactcttgt ccatgaagat agcattactc ttgcagatat cgaaggaaaa
    26701 acagctcagg agatcaaaga gtatctaaac gaacgagcac aagaagtatt taataccaaa
    26761 gcggctcaac taaatggtcc agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt
    26821 gttgtggact ctaaatggac agaccatatt gatgcaatgg atcaattacg tcagtcaatc
    26881 ggcttacgtg cttatggaca gaacaatcca ttagtcgaat atcaaacaga aggctataag
    26941 atgtttgaag atatggtagg cgccatcgag tatgaagtca ctcgaatctt tatgaaagct
    27001 gagatccgtc aaaacgtaca gcgtgaacag gtttctcaag gaacagcttc tcactcagaa
    27061 gacggagatg ccaaagaaaa tagtaatact agcgcgaaaa aacagccaat ccatgtgaat
    27121 aaggtcggaa gaaatgatcc ttgtccttgt ggaagcggga aaaaatataa gaactgtcat
    27181 ggaaaaaatg caaactaatt tctgacagca ggattggtga gacaaattct ttttttaaga
    27241 attcgtctca ccttctttct gttatttgct gattctgtta gactagatat aatgaaaaaa
    27301 gtatagtgaa agaaggaaaa aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa
    27361 tgcaggaaaa ggtcacaagt ttcaggaggt ctctttgact tagatcagct ggaagaagac
    27421 atcgcggaag cagaagcaaa aatggctgaa cctggttttt gggatgacag cgaagccgca
    27481 caaaaagtga tcagcgaaaa taacgctaat aaagaaacct atgaccaatt ccattctctt
    27541 gccgaagaac tagaagaaat cgaattgatg gtggagatgc tgcaagaaga acctgatgca
    27601 gcaatgcaag aagaagcaga aacacgtatc aatgcattag atgaaaaaat gaagacgtat
    27661 gaattatcta tgctgttaga cggaccttat gatcgaaaca atgcgatcgt cgaattgcat
    27721 ccaggggctg gcggaacgga gtcccaagac tggggcagca tgcttttacg tatgtataca
    27781 cgttgggcag agcagcatgg ctatcaagta gaaacactag attatcaagc aggtgatgaa
    27841 gcagggatca agagtgtgac cctgctgatt aaaggacata acgcttttgg ttatctaaaa
    27901 tcagaaaaag gtgttcatcg ccttgtacgg atttcgcctt ttgactcagc gaaacgacgt
    27961 catacttctt tttgttcggt agatgtgatg ccagagcttg atgaaacgat cgatatagaa
    28021 atcaatccag atgatttgaa aatcgatacg taccgagcaa gtggtgcagg tggtcagcac
    28081 atcaataaaa cagaatctgc tgtacggatc actcacttgc ctacaggaac agtagtagct
    28141 agtcaggcac aacgctcaca attgaaaaac cgtgagcaag cgatggggat gttaaaagct
    28201 aagctttatc aattagaagt ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa
    28261 caaaaagaaa tcggctgggg ttcacagatc cgttcttatg ttttccatcc ctattctatg
    28321 gtaaaagacc atcggacaaa cgtagagaca ggaaatgtcc aagcagttat ggacggtgac
    28381 cttgatttat ttattgacgg ctatttaaaa atgcatctac aataagaacc cattgttaaa
    28441 ttgaaataaa attgtaagat tttgcaagca tgttgaaaca ttgccatgct ataatagcta
    28501 aagactgaaa aagatatgga gagaacgcca tgattgaaat gaaggatgta atgaagaagt
    28561 actcaaatgg tacgaccgct atccgcaatc tctcgattgt catcgaacaa ggagagtttg
    28621 tctatgtggt tggtccgtct ggagccggta aatctacatt catcaaattg atgtatcgag
    28681 aagaaaaagc ttcaaaagga actttgaacg tcgcaggtca tgatctaata gcaatcaaaa
    28741 accgcgaagt tccttatcta cgcagagaaa tcggcgtggt gttccaagac tataaattat
    28801 taccaagaaa aacagtatat gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac
    28861 ctcgagatat caaaaaacga gtaatggaag tgctggacct tgttggtttg aaacacaaag
    28921 ttcgagtctt tcctagcgaa ttatccggtg gggaacagca acgtgtctct attgctcggg
    28981 caatcgtgaa tacgcctaaa gtacttatcg cggatgaacc gacaggaaat ctggatccag
    29041 aaaactcatg ggaaatcatg aaattattag atcggatcaa tgcccaagga actactgtcg
    29101 tgatggctac acataacagt acgatcgtaa atacgattcg gcaccgagta attgctatcg
    29161 aaaacggccg cattattcga gatcaagcgg aaggggaata tggatacgat gattagaacc
    29221 tttttccgcc atttattaga aagtatcaag agtttgaaac gtaacggctg gatgacagtt
    29281 gcttcagtca gtgcggtaac gatcacgttg actttagtcg gtgtattcat ggcagttatc
    29341 atgaatgcaa ctaaattagc tcaggatata gaaggaaacg ttgatgtatc tgtattcgta
    29401 gatatcggta caaaagacga agagatgaaa aagctagaaa aagaattgaa tgacttagat
    29461 catgttaaaa aagttgaatt ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa
    29521 atgggtgatt cctggaaatt gtttgaaggt gacaataacc cgttatatga tgtgtatgta
    29581 gtaagtgcta ctgatccttc ttatacaaaa caagtagcag aagaagcagc agatttgaag
    29641 aatgtttctc gttctgatta cggtggagct tcctcagatc ggattttcca aatcgctggt
    29701 gcagttcgga catggggatt agttgcagcg gcattgctgc tgttcgtagc gatgtttctc
    29761 atttcgaaca cgattcggat cacgattttg tctagacagc gtgaaattca aatcatgcgt
    29821 ttagtcggtg caaaaaatgg atatattcgc tggccgtttt tccttgaagg tggctggatc
    29881 ggtctgatcg gtgcaattct tccaattttg atcatcacat ttggttatgc atggtttttc
    29941 caattgatca atccatcatt attacgttcc cattattcat tgattcatcc acaaaatatc
    30001 gtttggaaaa tcaatctttt aatggtcggc attggtgtga tcattggttc acttggctcg
    30061 atcatttcta tgagacgttt cttgaaattc taataccaca atataaataa aatgagaaag
    30121 ttggacagtt tcgttccagc tttctttttt tattggctag gagaatagaa ggaagcggct
    30181 tttttccctt tactttaaaa aacaacttgg gtattcttaa gttggtagaa gaagaggtga
    30241 agccgtgaaa attatcgttg acaatttttt tacgatcctt ttgattatga atattttgct
    30301 ttcctttatc atcgtttttc gagaaagaaa ggaaactgct caaacatggg catggctgct
    30361 cgttttgatg ttcattccag ttgtaggatt tattttatat atttttttag gaagagggat
    30421 ttccaaagag aaaatctttg atttgaagat ccaagggaaa atcggtaaga acctggaaat
    30481 tgaagaagac aaacaggcat tgatgcgagg gctttatcct catccgccta ctggacaagt
    30541 agatgtgaaa caattgatct atatgctcac ggtatttgaa agtacgctct atacgactaa
    30601 aaatgaaatc actttattta cagatggtcg tgaaaaattc gatgcgctga ttgaagatat
    30661 caagcaggca gaagaccata tccacttcca gtattatatt tatcgcagtg atgcattagg
    30721 tgaagaagtt cgagatgcat tgacagaggc ggcaagacga ggggtaaaag tcagagtcct
    30781 tcttgatgca tggggttcca cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc
    30841 aggcggagaa gtcgcatttt tctttcctct atttgttccg tatattaatc ctcggatcaa
    30901 ttatcgcaat catcgtaaaa tcgtagttat tgatggggaa atcggatata caggtgggtt
    30961 caatgttgga aatgaatatc taggattggt aaagaaattt ggttattggc gagacaatca
    31021 tttgcgtatt tacggagaag cggtgtatac cttgcaaaac cgttttttga tggactggaa
    31081 ttctcagcat acaaaagaag taggctattc tcccaaattt tttccgtcca ttcacactac
    31141 tggtgatata gctgttcaaa tcgtgacaag cggaccagat accgaacatg aacaaatcaa
    31201 aatgacttat ttgaaaatga tttcgttagc caaacgggag atattgatcc aaacgcctta
    31261 ttacataccg gatggatcga ttcatgaagc actgaagttg gctcttcttt caggcgtaaa
    31321 agttcatatc caaataccta acaaaccaga tcatcttctc gtttactggg caacgtattc
    31381 tttcgctgca gagctgatcg agtatggagc aaggatcgaa acatatgaga atggatttat
    31441 tcatgcaaag acgatgatta tcgatggtgg cattgtttca gttggctcag caaatatcga
    31501 tgttcgttca tttcgcttgg actttgaggt caatacactg gtttatgatg aacgaatggc
    31561 ttcacgtgta agaaaagctt tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga
    31621 aatgtatgaa aaccgaggaa taatcatcaa gatgaaagaa ggattagcaa gattgatttc
    31681 gccgctcctt taaaacataa aaaggacatg agaaccatca acagatggag atctcgtgtc
    31741 cttttttaac ggggccattt ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag
    31801 cgaaattaac tttggggtat ctcttgtttt ttggggaaag caagaaataa gctaatatat
    31861 gctattaaaa gagacacatt tcaaagccag tatagc
//
LOCUS       DANKEF010000030        30858 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000030 DANKEF010000000
VERSION     DANKEF010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 30858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.030"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(118..663)
                     /locus_tag="PWZ48_001548"
     CDS             complement(118..663)
                     /locus_tag="PWZ48_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2261208.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(675..1367)
                     /locus_tag="PWZ48_001549"
     CDS             complement(675..1367)
                     /locus_tag="PWZ48_001549"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDL2261209.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1386..1670)
                     /locus_tag="PWZ48_001550"
     CDS             complement(1386..1670)
                     /locus_tag="PWZ48_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261210.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1683..2246)
                     /locus_tag="PWZ48_001551"
     CDS             complement(1683..2246)
                     /locus_tag="PWZ48_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HDL2261211.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2474..3136
                     /locus_tag="PWZ48_001552"
     CDS             2474..3136
                     /locus_tag="PWZ48_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HDL2261212.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3260..4456
                     /locus_tag="PWZ48_001553"
     CDS             3260..4456
                     /locus_tag="PWZ48_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HDL2261213.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4557..5933)
                     /locus_tag="PWZ48_001554"
     CDS             complement(4557..5933)
                     /locus_tag="PWZ48_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2261214.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5970..6836)
                     /locus_tag="PWZ48_001555"
     CDS             complement(5970..6836)
                     /locus_tag="PWZ48_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HDL2261215.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6925..7950)
                     /locus_tag="PWZ48_001556"
     CDS             complement(6925..7950)
                     /locus_tag="PWZ48_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HDL2261216.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7968..8642)
                     /locus_tag="PWZ48_001557"
     CDS             complement(7968..8642)
                     /locus_tag="PWZ48_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2261217.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIKGYIETKVG
                     QGYIVP"
     gene            complement(8826..9719)
                     /locus_tag="PWZ48_001558"
     CDS             complement(8826..9719)
                     /locus_tag="PWZ48_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2261218.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9793..11490)
                     /locus_tag="PWZ48_001559"
     CDS             complement(9793..11490)
                     /locus_tag="PWZ48_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2261219.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(11483..12796)
                     /locus_tag="PWZ48_001560"
     CDS             complement(11483..12796)
                     /locus_tag="PWZ48_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2261220.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(12796..16833)
                     /locus_tag="PWZ48_001561"
     CDS             complement(12796..16833)
                     /locus_tag="PWZ48_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDL2261221.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FHHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(16846..17304)
                     /locus_tag="PWZ48_001562"
     CDS             complement(16846..17304)
                     /locus_tag="PWZ48_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2261222.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(17602..18120)
                     /locus_tag="PWZ48_001563"
     CDS             complement(17602..18120)
                     /locus_tag="PWZ48_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HDL2261223.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(18122..18739)
                     /locus_tag="PWZ48_001564"
     CDS             complement(18122..18739)
                     /locus_tag="PWZ48_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HDL2261224.1"
                     /translation="MMNRSIGKKANDYFLEIIEWIPRLIHLQLLWFLCVLPVFTLGTA
                     SRTVLYMIYHYKKNEEKKLGSLFWKKFRENFSLYGKQDSLASVYFLLLLIDYQIFRYW
                     GGGIGYTLMYTTLFLLLLSALLFSYKILLQLEQKEASWITAFFLFFNDFKSALFYLAG
                     TLILLVAFWFAGPIYFALLGFSFLFYFQVLLFIHRTQEKVLKKEE"
     gene            complement(18736..19590)
                     /locus_tag="PWZ48_001565"
     CDS             complement(18736..19590)
                     /locus_tag="PWZ48_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2261225.1"
                     /translation="MMDYFKKTSVNRQTKKILSYVLMTVIGIILIIPLLWMVFTSLKP
                     MEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFIAYG
                     FAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGNAFN
                     IFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWNDFL
                     GPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFIEGM
                     DLTGGSKG"
     gene            complement(19587..20498)
                     /locus_tag="PWZ48_001566"
     CDS             complement(19587..20498)
                     /locus_tag="PWZ48_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2261226.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(20539..21873)
                     /locus_tag="PWZ48_001567"
     CDS             complement(20539..21873)
                     /locus_tag="PWZ48_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDL2261227.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKLFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            22081..22911
                     /locus_tag="PWZ48_001568"
     CDS             22081..22911
                     /locus_tag="PWZ48_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HDL2261228.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWSGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(23155..23473)
                     /locus_tag="PWZ48_001569"
                     /pseudo
     CDS             complement(23155..23473)
                     /locus_tag="PWZ48_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(23595..23789)
                     /locus_tag="PWZ48_001570"
     CDS             complement(23595..23789)
                     /locus_tag="PWZ48_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261229.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(24294..24479)
                     /locus_tag="PWZ48_001571"
     CDS             complement(24294..24479)
                     /locus_tag="PWZ48_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261230.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(25329..25670)
                     /locus_tag="PWZ48_001572"
     CDS             complement(25329..25670)
                     /locus_tag="PWZ48_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2261231.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(25670..25900)
                     /locus_tag="PWZ48_001573"
     CDS             complement(25670..25900)
                     /locus_tag="PWZ48_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2261232.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            26187..26525
                     /locus_tag="PWZ48_001574"
     CDS             26187..26525
                     /locus_tag="PWZ48_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261233.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(26730..26969)
                     /locus_tag="PWZ48_001575"
     CDS             complement(26730..26969)
                     /locus_tag="PWZ48_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2261234.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(26945..27292)
                     /locus_tag="PWZ48_001576"
     CDS             complement(26945..27292)
                     /locus_tag="PWZ48_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HDL2261235.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(28024..28605)
                     /locus_tag="PWZ48_001577"
     CDS             complement(28024..28605)
                     /locus_tag="PWZ48_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2261236.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(28788..29414)
                     /locus_tag="PWZ48_001578"
     CDS             complement(28788..29414)
                     /locus_tag="PWZ48_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2261237.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(29436..30767)
                     /locus_tag="PWZ48_001579"
     CDS             complement(29436..30767)
                     /locus_tag="PWZ48_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HDL2261238.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
BASE COUNT         9100 a         6596 c         5239 g         9923 t
ORIGIN      
        1 aaaaagaata agttttttta aaataacgta taaaaaagag cataaaacaa agtcgtttgt
       61 tttatgctct tcttttatta ttttgtgctt tttattttat caaatcactt tgttttttta
      121 taaaatttct gcgcctagtg tggactcaaa atgtctcaat gcccattcat gtccaacggg
      181 atcgaaactt gccacggaat ctttgaaaat atggagtttg tatcctagat tatacgcatc
      241 gactgctgta tgcaacacac agatatctgt acatacgccg gtcaaataaa tatctgtgat
      301 ctggcgttct cgcaaacgga tgtctagatc tgttccacta aaagcggaat aatgccgttt
      361 gtctatccaa taaacatttt cctcttgctc atagtcttgg tataaacttt ttaaagagcc
      421 gtacaattcc cgtccacccg ttcctatcac attatgcgga ggaaataagc ggttctccgg
      481 atgaaaggaa tcttttggat catgcgcatc aatggcaaaa acgacaaaat ccttttgatc
      541 aataaacttc tttgtatgag cagtcagctt tttttcaatt acttgtcctg ctgtcccagt
      601 agtcagcttt ccatcctctg caacaaaatc atatgtgtag tcaatcgaga tcaatgcgtt
      661 catcttagtc ctccttatac gagatgtttg acaaattcga tcaccatttc tgctgatcgt
      721 ttgcctgcgt cttcgataaa ttcgtcaaag ctttgtgtcg ccgagtgatc tgctgtgtcg
      781 ctcattgcac gtacgatcaa aaatgggata ttgaactgac gagctgtttg tccaacagcg
      841 gctccttcca tttcgcaagc taatgcttca gggaaattcg ttaagatttc tttgatttta
      901 tctggcgagt cgacaaatgt atcacctgta acgatcaatc cttcttttgc gttcaaattc
      961 gttgcttttg ctgctttaac catttcactg cgcaaatatg tgcttgcttc ataatataga
     1021 ggcattcctg gtaattgtcc tgggttgtag ccaaatcctg ttgcatctgc atcaaaataa
     1081 gctactttgt ctgatataac gatgtcacct acttgtaagc cttcaccgat tccgccagct
     1141 gaacctgtat tgatcaccat gttcacgccg tattgttgga tcaacagact tgtggtaata
     1201 gatgctaaga cttttcctat accggaacgt acgacgatca cttcgtggtt acctaatgaa
     1261 cctgaaataa ataaagcgcc tgcgcgttcc catgataatg gttcgcttag gttctctctc
     1321 aaaattttta cttcttcttc cattgcgccg ataataccga ttttcatctt tttaattcct
     1381 tctttctata taaatgcgat tgctaaaaaa accacacata acattaggat aacaataata
     1441 agagatttca tcaaaaaaga gttccatttg gtcgtctttt cccgttcgct gatccttgtt
     1501 ttcgtgattg gtttattctt tttgctttct ttacgataaa agctggcaat ctttttttct
     1561 tcttgctgat aagctgtttc tgcttttcgc tgttttttta atgactcgct tgcttgtgct
     1621 tgttgtcgct tgcgaagttc gcttcgcgta ataagcggtc ctttggccat aagccgatcc
     1681 ctctactttc ctttggtcaa tagttcccag tattgaatcg cataaatcgt ctttgcatca
     1741 caaatcaact gatctttcat cgcttgttgc gcctcttcaa gagttagatg atacaactcc
     1801 agtacttcgt cttcatcttg tgctaaagga ttctcgacct tctctacccc ttgtgcatga
     1861 taaatatgaa ggacttcatt tgcaaaacct ggtgaaagat acatcgaagt aagatggctc
     1921 agcgacttcg cacgatagcc agtttcttct tctaattctc tagctgctgt catttcggga
     1981 ttttgtcctt ctcctggatc gatttttcca gctggaatct ccaaaatcac tttttctaaa
     2041 ggttttcgaa attgtttgac aagaagcaga cgatcttgtt cgtcaaaagc aatgatcccg
     2101 acaccgccag gatgaaatac cagttctctc ttcgaagttc ctccatctgg caaacgaact
     2161 tcgtctacag caacatcaat aatttttccg ctatagattt ctttgcgact gattgttttt
     2221 tcttcaaatt gctcattgtt cagcatatct tctcctgctt tctttgtaat cataactgct
     2281 tttttttcat ttttcaattt ttctcaaaaa ctccttatta gtttaccata gataaacctt
     2341 agattttctc taaatataga aaatccttct ttagtccgat actctttcta gtagtccgta
     2401 tgctaaaata agtatgtaat cgaataacga aaagaattca atactcaacg ataaatgaca
     2461 tggagggttt attatgataa aacgaaattg gccgtggctt ctagcattgg tcttgctatt
     2521 tctctttgtc cgaaacaata gtttttcttt tgttctagga cttttattga taggtgtagt
     2581 tttagttttg tggggactct ttggttcgcg caggaaaaag ggaaaacaag aagaaattcc
     2641 tgcactgact gacgaacttg aaactcacta tgaaaaaagc ggtatgacag cgaatgaaat
     2701 ttcatttttt cgacaaacaa tgaatcaaac aaaaaatgag atcaatcaat tgcaaatgaa
     2761 tatgcagcag acagccaaac ttaaagcaat cgatctccgc catgatccag tcaaagcagc
     2821 gaaagcttta ttcaaagaat tggtgaaaga accaaaccga ctgcatgaag caagtcagtt
     2881 tctttatacg catctgccga atctagttga tttgacaaat aagtataatg agatcaatga
     2941 ccatgagatc aaaaacaaac agacctatga aaaactagaa gaaagcgcgc agatcatcga
     3001 ccagctgtca gctttaatcg cacaagatta tcaaaaattc gttgctgagg atctagatga
     3061 tatcgacgta gagatatctg ttgcgaaaca gagcttaaag cgtgataata aatatgaacc
     3121 agaacaaaag gattaactct tttgttcata aaagaagact cggaacattc agatgatgtc
     3181 tggctgtttc gagtactttt tctaaagtag agacattcat gtaagaatcg agaacagatt
     3241 caacaaggag gaatttttta tggatcataa accgactgaa acaaaagatg ttacaccagt
     3301 taacgataca cttgaagatt tattgaacaa cccgttttca actccaacag atacgctgac
     3361 cacttcacaa caagcggaga ttgatcagct ccaagagcaa cagacagctg ctcgtttaat
     3421 tgacaaacta ccggcggaac gccaagaaca agctaaacag ctagcagcaa aaatcgatgc
     3481 taatgatgca cagtctgtca tcagctacgg atcggctgcc caagcaaaat taagtgaatt
     3541 ctcacaatcg atgttgaatc atgtgcaagc acaagatatt ggtcctgtag gagattcctt
     3601 aactgaactg atgtatcgtc tccaagaagc caatcctgac gaactgcgtg ccggagaagg
     3661 gaacatcttc cagcgtgtct tcgggaaggt caaacaatcg atctatgaag tgacagcaaa
     3721 atatcaaaag attggtgcac aaattgacaa gatttcagtg aaattagata aagaaaaaga
     3781 cggtctgcta aaagataatc tgatgttaga acagctttac cagaaaaaca aagactactt
     3841 tgatgcactg aatatttata tcgctgctgg agaattgaaa atggaagaac ttcaaacaac
     3901 gatcattcca gaagcgatga aaagagcaga agaaactggc gatcagatgg atgttcagat
     3961 tgccaatgat tacacgcaat tcctagatcg tctggataaa cgtacgcatg atcttcgctt
     4021 agctagacag atcacgatcc agcaagcacc acaaatccgt ttgatccaaa acacaaatca
     4081 ggccttggct gaaaagatcc aagcatctat cgctacagcc attccgctgt ggaaaaacca
     4141 agtcgtgatc gcattgacct tgcttcgtca aaaagatgca gtaactgctc aacgccaagt
     4201 ctctgaaaca acaaacgact tattgaagaa aaattcagaa atgctgaaaa tctcagccat
     4261 cgaaacagcg aaagaaaatg aacgtggaat cgtggatatc gagacccttc aaaccactca
     4321 aaatgatttg atcgaaacaa tccaagagac attgcgtatc caaaaagaag gaaaagaaaa
     4381 acgtcgtcat gcagaagtag agcttggaca catggaagaa gatctgaaac aaaaactgtt
     4441 ggatttaacc caatagatca cctttcgctt gttactaaaa caaacgccca acaacagttt
     4501 attacaactg ttgatgggcg tttttcttat ctttcttcta acttctccat tggagtctat
     4561 ttctcattca tcttgctgtg tccatagcca tagccaaaat aaatgatcag tccaataacc
     4621 attacgatac cgaacacgat ccatgtcaca gtttgcagac gaagcatcaa tacaatacaa
     4681 gtcaagatgg agacgattgg caatagcgga acgaatggca ctttgaattc tccagctttt
     4741 ggtttgccaa acatttttct caacttcagt aagcctagac taacaagcat caaataggcg
     4801 agcgccatga tattcgttag ttccgctaat acattcaatg ggaaaaatcc ggcaaagaat
     4861 acagctgcca caccagcggc ataagtcgct tttttcggtg tacgatgctt ttgatcgatt
     4921 tctgtcataa acttaggaag taagccatcc ttgctgacag caaacagtaa acgagcaagt
     4981 gaatacatca tcgagatcgt taccgtcagt agcgttaaaa ttgcccctac agcgatcacc
     5041 gcgcccactg ttccatgtcc tacatacctc attgcaaaag ccactgggtc attgactcct
     5101 agttcagtat acggaacgat tcctgtcaag atcagcgtca cgatgatgta aaggacagta
     5161 gcaatcagta tcgaaccaat gattcctctt ggcacatctt tttgcgggtt tttgacttcc
     5221 tcggctgcca ttgcaacggc atcaaatccc aagaaagcaa aaaacacaag agctgcacca
     5281 tctagcacac cggaaaaacc aaaaggcatg aatggctgcc agttatcagg tttaacgaaa
     5341 aatattccta caatgataaa caacgcgatg ataccaaatt tgacatatac catggcatta
     5401 ttcaaacgta atgctttctt ggcttcaagc gacacgatgt aagtgacgaa tatcactacg
     5461 agcaccgcaa tcaagtcaat gtatgtacca ttttccggat tgtacccgcc agttaatgct
     5521 tgtggaagtt cggcccctaa acttttcaaa aaaccttgca tatagccaga ccatccggaa
     5581 gcaacagagg aaaccgctag taaaaattcc ccgatcaaca accaccctgt cagccaggca
     5641 gcaaattcac caaatacaac atacagatag gcatatggcc caccaatcac tggcactcgc
     5701 gaagcaaatt ctgcgaaact tagtccagac aaaatgacaa cgattgcagc taagacaaat
     5761 gaaagtgaca gtgctggacc agcattttga ttggccgcaa cccctgtcac cacaaatatt
     5821 cccgtaccga tgatcgcacc aattccaagc atgatcaaat cagacgtttt tagttctttt
     5881 ttcatctcac tcgtatgatt gagattgatc tctttcttac gaaacaaatc cattgaagtt
     5941 cctccccatt ttaggttgga aaacagcgat taaactgttt tcatataccc taaaaggata
     6001 ccacctaaaa tgactaaaag gcaaccaaca ataacataaa tgagctcttt tttcgttttt
     6061 ctttcgccaa ggatgaaaat cccacctaaa gtagagataa tgatccccat ttgtgaaagt
     6121 gagaagccaa cagctaaacc aagtgatttg actgttagaa gcatgcagat gtttcctaag
     6181 ccccataata atccacagat catgtttttc cacacaaact gatcgacttt aactttacgg
     6241 aaagcaaaga agcttgcgcc taagatcatt ccgatacttt gaggtaagat aattgacatc
     6301 gcatccaaat tcgcccagtt gacgatgatg gtatatacac cataaccaat cgtagagaag
     6361 atcaatgctc taaaaccctt accaaaatca agcattttat tatctgtatc tatttttcct
     6421 tcgtcatctt gtcgggcagt caaatacgca ccgcttacaa gaagagctaa agcaataata
     6481 ccgtatacat aatctttcgt ttgtgtccat tcatggaaga aaacggctcc cgcaatcgta
     6541 ttcaaaatta attgaaaacc agtagacaat ggtagcccta cagaaacacc catgtacttc
     6601 attccatgga actgtccatt ttgtccaaca ctccaagcta agccggataa aaatccgatc
     6661 aagaaaatcc aaggggtgat tacaggacta acaacgaaaa agacaactaa agaaaacaga
     6721 aatgcaccga tcgtcattcc tagtgtttgt tgattggcat cgccaccaat tttaccactt
     6781 actaaaccaa tactacccca tgccaacata gggactaatg ctattaaaat ttccataacc
     6841 aatctccttt tatttcacat gataagaaac ttatcagcga aaacgttaac aacacaagca
     6901 ctttttttcg tttctaacga ttttctaacg tttttcatta taaaaattta agattgggaa
     6961 aaataaagtc attgttgtcc cttttccttc agttgattca gccgtcaaat ggatacctaa
     7021 tttttcggcc agattttttg ctaaataaag tcctaatcct gttgaatgtg ttttgctaag
     7081 tcgaccattt ctcccagtaa atcccttatc aaagatccgt ttcaagtctt ccgctgggat
     7141 accgatccct gaatctttaa ctgaaaggct gactccttgc gcttcctttt ctatgctcac
     7201 aatgatgttt ccgtgatccg gcgtatattt gatggcatta ctaagcaatt ggttgaagat
     7261 aaaagcgatc catttagcat cggtcaaaac agtatgatcc tcaccaacga tctcgtagcg
     7321 aatatttttt tggataaaat aatttgcctg actgcgtatc acaggctgaa tgattccttt
     7381 tagtccatat tcttggatga ggtagtcccg cgagaaactg tcgagtcttg cataatacaa
     7441 aacttgttct acgtattcat cgatttttga taattcattt tctagtaatg tatacttttg
     7501 atcgtcaata tcgaactcga tcgaacgaac tagcaattta gaagcggaaa gtggcacttt
     7561 gatttcatgg acccatgagt caatataatc tttttggtct tgttgattac tgattgctga
     7621 ttgcattact tcttggtgtt cacggatcag ctgattgata tattcttcta ccagtttttc
     7681 ttcttctgtc ctcccaccac tcaaataatt ttgcaaaggg gaagtttttt gtatgtcggc
     7741 taattttccc caccatcttc tcttgctcaa gtaaccaccc agcagataaa aaatcagaaa
     7801 gacgccctca atcaaagcta aataagcaat tgtgctcaaa tcaactttga tgtttggcgc
     7861 cagccacatc acaaagatcg tcaatgcaat aaaaaacagc catcccaaga tcaatgtcca
     7921 ctgatctttc aaatatttaa aaaaattcat cttaaccgct catcctttca gggaacgata
     7981 tatccctgtc ctacttttgt ttcgatatat cctttgatac ctgcctgttc gatttttcgg
     8041 cgtaaccgat tgatgttgac tgtcaatgta ttgtcatcta caaatcgttc gtcatcccac
     8101 agtgctcgca gcaatttttc ccgacttaag atctttccat tgtgcttcat caaaataaat
     8161 aatagacgat attcattttt gctcaaatca atgatctctc cgttgatttc catacttccg
     8221 ttgtcaacat tcaacgtaat gccattgtgg ctcatcattt cactagataa ttctgtgtag
     8281 ttgtaagaac ggcgaagtaa tgcattgatt tttgcaatca gtacatcgat ttggaaatgt
     8341 tttgtcacaa aatcatctgc ccccatgttc atagccatga tcatatccat attcgtattc
     8401 ctgctggaga taaaaatgat cggtactttc gatacttcac gtattttttg acaccaataa
     8461 tagccatcca aaacgggaag attaatatct agtaatacaa gctgtggttc ttctctttca
     8521 aagtctgaga acacttgatt gaaatcggtc gttccaaagg tttcaaattg ccatttctca
     8581 agttcctcca tgatcaattg tcgaatgatc tcttcgtctt cgactaccat gatttttgcc
     8641 ataaaaaatc cctccatctt tcttcttgct actacaatat caaagaagcc cttgtgaaac
     8701 aagttggctt acctggaaaa tgtcatttta gcaaacgtta aaaaaaactg gaaatatgcg
     8761 atttaatcgc gtttgtctct tccaagcagc ttaaatttat tatgttattc ttaataattg
     8821 gtctattatc tgttattttt ctttcgttgt ttttgtcttt taaattttcg ataacaccaa
     8881 taaccgaaaa gcgttagcac tagcaatata cctagaagga gtataagtaa tcgataaaga
     8941 tggtattttt ttgtctgttc tatctttgaa gagacttctt ctgtaaaggg aactcgtctt
     9001 cctgtcacta gtaatcggtg agtgttcacg gtgtaaggtg tacaagtgat aagtgtgact
     9061 agatcctgct tttctctaat tgctaatgta tttgtttttg ttggaaggac aacaattttc
     9121 tcaacgactt cataagcgag catccgacca tacacttcaa gatagaatcg gtcatcttgc
     9181 tccatttctt caagatccgt aaagagtttt ttctcagcaa taccgctatg ggcggtaatc
     9241 acggaatgtg tactctctcc tccagttgga aaagaggtgc cttgcaggac tgtcgctcct
     9301 ctatctaaca aaagatcatt cgtttcatca aataaaggca agctaacatt gattttcgga
     9361 atatagatgg ctccgatcat atgttgttcg taatattctt ttcctggatt ttggacattt
     9421 ctaaccgctt gctcaaaagg atctttgact tgtcccattc ctgggattac tgatgtatga
     9481 gcaagcgtct gatttttctt ttcatgcact gctaattgtt gctcatgttt ttcttcttta
     9541 tcgcgttcaa gctgcttttg ataattctcg atccttcttt gatccaaata attgctgaga
     9601 gcatctgcta aaaatggata tgtaaaaact gcagctccgg caagaaacat cactgccata
     9661 aataattgta ataaaagact gatttgggtt tgaggtctat tacctatttt ctttttcatt
     9721 ttcttcacct tcttatttaa aaaaactgca gcaaccttta taaaaccgat tgctgcagct
     9781 tatttttgtt gcttatactt cagacttcat tttagattta cggtaccagc tgtacgctcc
     9841 aatcatcaaa cttattccga ttagtaagaa tgccaagagc ccgttcccac cagtagcagg
     9901 aagtgacccg cgtttcatat tcggtactct ttctggccct ggattttcta ctcctacgat
     9961 ctgaatttct tcattatagc ttccataaga gatgataaat tcagtaaagg ctgcctcacc
    10021 tgttggaagt acataacctt ctggcgccat ggtctctttc atttgatagg ttccataatc
    10081 taacccttga acctcaaagt atccatttcc atctgcgaca aattctgttg cttgtccgct
    10141 tgttgcccac tcaattctat ctgctcctga ttcataaaca gctaaaggcg actccccttt
    10201 ataaaatatt gcgtattcag aacggtcttc attccacaat tcgaatcttg catctttaag
    10261 caattcactg cttgatgcat catatttttc aaactttctt ccacctgttg tgactttctc
    10321 tggcggctct actccttctt cataatcaaa ttcatcattt cgtccgattt caataaccgc
    10381 tgtgttgtta tggaaatcat ccgcttctag ttctgtggaa accttcaaat tatacgtgat
    10441 cgttaaatct cctccacgtg catctgctaa tctacctaaa gtatctactt tggctactcc
    10501 attttcttct gtcaaaatcg tcacgacaaa cccgttgctt actacttcta ctatataatc
    10561 ttgtcctttt gttaaaatcg tctcgccggc tcgcacttct atttcctgat cataataagt
    10621 tagaccaact gtaggcgtat ctgtgatttt gtaatttttg ataacagcgc ttccgttttc
    10681 aaggacagaa tcgatgaaat aaggaatagg tgctgtgatc gtgtaagaag ccgtttttcc
    10741 tctttctaga tcaatataag aaatcgtttc ttgttctcca gcttcattaa taatcgttac
    10801 ttgtctaagg tcgctttcct ctacattcat ctgtttatct gcttctctga tttcgttttt
    10861 cggatacaaa tgcacatctg tattgtatgt gtctttccat gtagaaccat cccacacgcc
    10921 atccaccata tcaggcatca tcactggcat cataacaacc attggagacg cgattctgga
    10981 aatatgagct ggcgaataag tttctaagaa aagatagact ttatctctag aagaaggtgc
    11041 ttcttccgtc acttggacgg tagagaatac cgcttctcca gcttcattcg ttgtttgttt
    11101 gtcaatactt gtcagtcgat attttaaaaa ccaactttct gtgtgccgtt tttgaatcca
    11161 ttcaatcgct tcggcagaat taagaccgtc ttctcgttcc ggtgttaaag gattatctcg
    11221 aatcaaatcg taatatactt ccgttacgtc aaacaaatca aaatcgactc ctggaatatt
    11281 ttcttcacca aaatccgtag aaaccaatcc gctattcggt cgttcttccg gaacagatga
    11341 gaaacctttt ttgtgtaatg taatcgtcac ttcacttggt ttttgtgcga cttcttcggc
    11401 tttgactagt tttccgttcc cgaaaatccc tattcccgca gtaacgagta acagtacact
    11461 cattcccgtg acaatcattt ttttattcat ttttttctcc ccgttctttt tttattaata
    11521 aaacagcacc taacaataaa agaattccta gtaaagaaat caacgttctt gcttcattcg
    11581 tttgcgggaa tacggaccat tttcctgatt gacctggatt gcttggacca ctcggctctt
    11641 tcggtttgtc cgttgtttct tttacggtct cgttataaac acgtggtgtt gcggattcat
    11701 aggcagagac tggaactttc ccggattcta attcagccat ctcctgtccg ttaactacta
    11761 catattgtgg ttggcctttt gcatccaccc aaaattcagg gatgatgacc tcgattctct
    11821 tacttgcttc atccatctcg taaccttcca caccttttaa ttcttcaaaa taataggtgc
    11881 cagaaggcaa gaaacgttgt cctgtcgtaa caaggccctc tttatccgaa atgaatttgc
    11941 ttacattttt gtcattcaaa ggatcattgt ctgctggttt gatccattga tttttcaagt
    12001 cattagcagg agccatatct aaataatatt tccccccttc tatcatctga tatagagcaa
    12061 aaacagcacc ttctaatggt tttccttttt cagttgttcc tctttgacgt ccatacttga
    12121 agaagtatgg atctcttaga taaccgacat ttttaggata gatatgaatc tcttgcagtt
    12181 cctcggttga ttctgtcggg tgataaattg gcaaaatagc agcaattggg atagctgtct
    12241 gatcgatatc aacatccaat ccaacttctg gatcaatcga ttcttcgaaa atcaaataaa
    12301 ctgcatcccg atcttggttt ttcttaggtg ctttgatacg tgcaatcccg tctcctaatc
    12361 cgccaaccgt atctttccca gttgttaccg aagtccctat ttggacaagc ctttcttctg
    12421 tgattagctg acggatattg tttctgttcg tttcagcgat ttgctggacg aattctttag
    12481 gagtcatgtt ttcagcagct tttttttcat gataataatc ggtcatgtcg taaatggcaa
    12541 atgtcgctcc atttagtggc accgtctgtg aaatgatatc tgctgtttcc cctgcttcct
    12601 cagagatagc caagccatca ttttcatggt attcaaattg atcgttataa tcgatatctc
    12661 gaatcatccg tttatgcaac acgaattcca cgacatcttc ccctgattct tctgccgaca
    12721 tcgcatgtgg aagaaggact gctgcaagcg gtagtaaaac aagtaattga agcatcaaac
    12781 acaacagctt tttcattacc ttgctccctc ctttttagat tgtctgacaa acaaaccata
    12841 ggctgctcct gcaagcgcca tgaagaatgc agcaagcagg atatagaata atcgtccgga
    12901 tcctcctgta gcaggcaacg gattttcagg atcgttcccg acactaagct gtaatctgat
    12961 tcgatttgtc tccaaaagtt catattctaa ctcttgctgt agatcaggcg tctctgcttc
    13021 ttcgtttgga taatacgaaa tctcaacttt tagtgtaccc gcttcttttc ctggaactac
    13081 ccgatcattt tctatgtcag gttcttctcg ataactaatt tctaaaataa agtaacctgg
    13141 aagttttcta tagctgtcag gtgacttcac ttccttgatc cgatagaccc cttccaacaa
    13201 gtctgaagca ttgaataggg aagaatcgtt tggatctggt gccaagcctc cctcgatgat
    13261 ttcatactct cctgcactat tttgtttctc aattgaaaat tctgcgcctt caagacgtcg
    13321 gctattcata ctgtcaattt tcaaaagttc caaatcgatt ggcgctagtt catcttctaa
    13381 ggttacttgt agtacaccat cgatgatttc atagccttcc ggcaagggag cagtttcgtc
    13441 ttcgatgacc gtatcttcaa tgataaactt accttcttca ttcagtgcaa actggaacac
    13501 ttgatcagat agccgatacc catttggtgc gacagattcc gagacctcat attcaccaat
    13561 agacagatgc agaggttggt cgtaatttaa atcgacatac ttttctactc ctacggcacg
    13621 agaatagaag tatccgatcc caggcaaacg tttcgattct tctggttcta acggattacc
    13681 agtgtatgct cgatacagcg ggaaggaaga atcccccact ttcctgatat cgaaacctgc
    13741 accatctaat gtatggcctt catggtctaa ttttctaact cggatctccc gataatcaaa
    13801 cgtattgtaa gcagtgaatg ctacccataa acgatcctct tcatctgttg tatacgtgag
    13861 atattgccaa ttttcttcac ctgtttgctt gagttggata gtccctatag cattatcgta
    13921 aagtcgcaag atccatgtgt cattttcatc aggttctttg aaaccttctg ggttctggct
    13981 ctcaatgatt ttgtattctg catagttggt agtttcttca ctatccttat aaacaaatga
    14041 atctggctct ccgattggat gaataacttt ctcaccatcc acgattacat atttcccgtc
    14101 ttcatctact gaatagaacg tgagtctccc tttctcttcc tctacttcgt tcacaccaga
    14161 aagccctttc gctactggat atccgatttc cagtccgtta ccatctgttt cataaagttc
    14221 aaactctgcg ccattgattg gcatattact gccttcaatt agtttattga tattcatgtg
    14281 gattggcttg aattcatttc gcatttccag tttgacatac aactcatcat actctgtgcc
    14341 ttctatgtta ggctcttgta caacagaaag cttgtgatat tctccttcat aataggaatt
    14401 cggatcttta ccttccggtc cgccgactct cgaaccagat gtatattcat ctacaagctc
    14461 gtagtgacct aacatctcat cttcgttggc tgcatatttt ttgatttgga attgatacgt
    14521 ctcatctact aactgataac gtggtggtcc agcgatctct ctgatccaaa atatgtcgct
    14581 gactgctcca gtttcatctc ctaaaatcgg catcttcgtt tcaaagatcg ctattccgtc
    14641 cgtggtagtt ttacttgttt cgattggttc ggcttcccca ctctctggat tttggtcata
    14701 taatgcaaat tcgactccag ccaacggctc gccattttga cccttcttgc tgacttggaa
    14761 attacggcct ttatgttcgt aacgaatgga agaagatggg aaatacatcc gtccagtagt
    14821 gatatcattc tcataatcct ccaggttctg aatataggcc atattgttca ctgtgcaaaa
    14881 atttccgtct tgagctcccg catcaagttt tacttggtac ttgatcctga ccatttcccc
    14941 ttcacctaaa gaaatcggag aaaatttata agcttggttt tcccgtccga catacgttgt
    15001 cggagtcgcc actacttctg gtggggcttg tccatgacgt tcttcatccc atatttctgg
    15061 ctcttggtac ttcatgccgt tttctcctgg atctagatgg taaaactgaa tatctgccga
    15121 tctgccagct ccaaatggtg agacattaaa accagtttca tcataaagtg tggcttgctg
    15181 gatcgttctc ttgaaggtag aagagatatc ctccaaggct gtaacaatgt cattcttgtt
    15241 gttcgcattg taatatcgcg gtgttacgcc atcttcttct aaatcagcaa tatttttcaa
    15301 tacatttcgt ccaatagcat ctagtcgttg tctcccgcta acgtttccat cgattcctaa
    15361 accaatggag aagatacttg gcgggttctc ttgatacttg tttttcagcc aatgatgata
    15421 agcaactgtg ttgatcgcag atgacttttc ttgcgaagta ggactgccat tcgtattgtt
    15481 attagaagaa ccatcaccaa cccagtgagt cgctttatta tctccttctt ttacctctcc
    15541 ataacgatag tgtacattta cttcatcttc tggacggtta aagtcagatg cataggtaac
    15601 tggatgagta aaccctgcat tagaagtatt cccagaaaac tcctcaaaat ttctgaacaa
    15661 aacgtcatta tcttcagcaa tcttatcttc catcacagtc cagttgctcc aagctccatt
    15721 agtgctcgta cgataacttg actgaatcgg tgcataactg aaggtcggtg tcccatctcc
    15781 cacaacgatt aggatttttt ctggatttct attttctccg ccattgtctt tatacaatgt
    15841 ttcatagcca tttttaagcc ctagtgtaag cggagttcct ccgattggtg tacgatttaa
    15901 tgctgtattc tcacttcccg tcaaacgatt gatatcattt gtcaatggaa agatgtccga
    15961 actgatttgc tcatggttat tgatagctgt actattatgg tgaaaattca ccataccgat
    16021 tcgaatatcc agactcgggt cagagagcag atctttactc atttcgttta ctgcctcaat
    16081 caacgcagca tttttagttt gactattggt cccagcggtt aattcactca tactcgctga
    16141 cttatctaga acaaaaacga tatcgattgg actgatttcc tttttctcgc ccccgatgac
    16201 atctagatag acattgtaaa gggttgttgc atcttcagga tgtagatcag atgctgtgct
    16261 gcttggcaga acaggttcta cccatttttt gatgtacgcc ccgttataat ccaagtaact
    16321 gttttcaaaa ttccgttcat ttccccaaag ctgaacggtt tgagctggaa cgtttctatc
    16381 ttcttcgcct gcagttgatc cattattagt agcatagtcg taatttcgcc agttcgctcc
    16441 cccgtttgta taatcactat catcgatcaa gttctctccg tcatatggcg agcctttgtc
    16501 tccttgatag cgttcattgc tttgtgtcca atttcctaac acttttgtat agtcattcgt
    16561 tgtaaacttc gggtagcgat gctgttcgtt ttcaacttct ccgctaggat agatatattc
    16621 ataggggtct tgcgtagctg ttggcccaag acgctccaaa ataccgttga ataactggcg
    16681 ttgaatgcgc tggagtgctt gaccgctggg tacgattggt tcgtccagct tttccatctc
    16741 attttcttcg ctacgttgaa tttcagcgga atcttctgta tcagtttcat tttcttgtaa
    16801 ttcttggtta tctattcttt catcaaccac cacttgaaga ggtagttacc cttcgttcac
    16861 atcggggatc ttttctgttt caaaaatcaa tgtgccggtt tctgcttctt ttgaataaac
    16921 ttttccgaca taccattctt gaccatcgat cgtctcgact tttaggtctt ctccatcttc
    16981 atcataggag gatagacctg ttccacgaac gttttttacg gtaccaatcc cggcggctgc
    17041 cgcatcgatt gctaaacgta ttctaccttc attggcactt tccttttttt caaaagccag
    17101 atgccattca agggtttctt ctttttcaat atacttccct tcaatttttg catagtcctc
    17161 atctaagaat acctcatcct cctttgtttc tgttgactca gcagctacat ccagggtcgt
    17221 catatgatag actgactgga aatatggtcc aaatagcaaa ctgacagtta ctaactgcaa
    17281 aagcttcaat tttaaccctt tcacatcctt atactccttt caaaaatatt tgtcttcttt
    17341 cacaacaatc aacaaaacaa tgcccatcta attataattt aatttaattt taattttaaa
    17401 tcaaatgata tcctttttag ttaaaaaaca ccaattatac aacatttatt attaaaatta
    17461 aattataaaa ttttataacg ttctcattta acaaaactta agttattaag acaaatgaat
    17521 aaacaactac gctttcctcg aaacaaaaaa ataaagcaca aatgcgcatt tacgcactca
    17581 tgctttattc gtacaattaa ttcagataac ggcagcgcat aggatgccaa taacacttcc
    17641 taaaatagct cctcctaaaa tgtcagtagg ataatgtaaa agtaaaaaaa tcctcgaata
    17701 agcgatcaaa caagcaaata aaaaggctaa tattcctgca actggactta attcgaacaa
    17761 cgcagctact gataaaaagg ctagcgctgt atgtcctgaa ggaaaagaag aagtcttcgg
    17821 tttacttgtg taaaggacaa gccgacgatt tttttcataa ggtcttttcc gtcttgtcgt
    17881 ttttttgata aaaagcaaac caacaaaaaa atagatcaaa ctgccgataa gaatggctag
    17941 accatattct ctagtatcag gtatcaagac taaaacagca gcaaggagca accaaataaa
    18001 accaccatta gagatattac tgataaattt caaaaaattg atttttcgac tatccgtttt
    18061 ccgttgcttg gcgaacttgt tgataatact atcatcgatt ttagcaatca aacgcaacat
    18121 gttattcctc ctttttaaga accttttctt gcgtacgatg gataaaaagc aggacttgaa
    18181 aataaaacaa gaaactgaaa cctaataaag caaaatagat tggtcctgca aaccagaatg
    18241 cgacaagaag gatcaacgtg cctgccaaat agaaaagcgc gcttttgaaa tcattaaaaa
    18301 acaagaaaaa tgcagtaatc caagaagctt ctttttgttc cagttgaagc aagattttgt
    18361 aactaaagag aagcgcgctt aatagaagca aaaacaacgt agtatacatc aatgtatagc
    18421 caattccccc accccaatag cggaaaatct gataatcgat caatagaaga agaaaataaa
    18481 cacttgctaa actatcttgc tttccataaa gagagaagtt ctcacgaaac tttttccaga
    18541 atagtgaacc gagctttttc tcttcatttt ttttgtaatg ataaatcata tacagaactg
    18601 ttcgacttgc agttcctaat gtgaaaacag gcaagacgca caggaaccat agtagctgta
    18661 aatggataag acgtggaatc cattcaatga tttctaagaa ataatcattg gcttttttac
    18721 caatcgatcg attcatcatc cttttgaacc tcctgtcaaa tccatccctt caatgaaata
    18781 acgttgtgca aaaaagaaca gcaaaatcgt cgggatcaag acaagtgttg ctcctgccat
    18841 cagataattc cattgtgtcg ttgcttggtt ctgaaaaact tgcaagccaa tctgtaacgt
    18901 gtatttttct tgatcttgga tatataacag aggacctaag aaatcattcc aagctgcgtt
    18961 gaaggaattg atcgcaatgg taatcaaggc aggtttcgtc aatggaagca tcaaatgact
    19021 ccaaatatac aaatgattcg ctccgtcgat ttcagcagct tcgatcaatt cattattgat
    19081 cgataagtaa aattgacgca tcagaaatat attgaacgca ttgccgaaga acgatggaac
    19141 gatcaggggc aaataagtac ctacccagcc gatcttggag aataatacat attgagggat
    19201 catcgttaca aaccccggga tcatcatcgt agaaagaacc aaagcaaaca tcagcttttt
    19261 cccaggaaaa tcaagcttcg caaatccata agcaataaaa gaatttgaaa ggacattacc
    19321 gatcactact agcacagtga tgaataatgt atttcttgca tagcgccaaa aaggaaaggc
    19381 ggcaatcgta tccagatagt tttcccatac gattttttct gggaaaaagg tcggaggata
    19441 acgaacgatt tcttccattg gtttcaatga agtaaacacc atccaaagca atggaatgat
    19501 caaaatgatt cctattactg tcatgaggac ataacttagt attttttttg tttgccgatt
    19561 cacactggtc tttttgaaat aatccatcat ttgccgtcac ctccagaata atagacccac
    19621 ttaggtgcaa gcttcaaatt gacaaacgtc agaaccaaca cgatcagaaa caggatcatt
    19681 gacatagcca tcgcataacc catatcaaaa gctacgaacg ccttgttcca catatgcaga
    19741 ttataaaaaa gcaaagaatt tgcaggtccg cctgttccat ttttcgtcat gacataagct
    19801 tcttgaaaaa tttggaaaga accgatcgta gaggtgatca catcgtaaaa aataatagga
    19861 gtgattaatg gcaaagtaat acttttgaac tttccccaaa cgcctgctcc atcgatttct
    19921 gctgattcat ataaagatct aggaacattt tgtaatgtag ctaagtaaag tagcattgct
    19981 cctccagaac tccatatttt catcaaaatc aacgcaggtt tcgtccattg tggatcaaac
    20041 agccaagctg gtccagggat atggaaccag cccagaaatg tgttgatcaa tcctgcagat
    20101 ggagaaagca gctgcatcca taagatgtag actgctactc ctgataagac tgccggtaaa
    20161 tagaaagctg tacggaatac actgattcct ttgatttttt gattgagtac tgtggctaaa
    20221 aacacactga aaatcgtcgt cagcggtaca ttgaagatga cgaaataaac ggtgttgtat
    20281 aacgatttcc aaaataaatc atcgtgaacg aacattcttt tgaaatttgc tagaccaatg
    20341 aaattcatct tggatgtcaa attataatcg gtgaaactac caataactga aaaaagcata
    20401 ggccctagca taaataccaa gaacccgata ataaaaggcg agataaataa ataggcccat
    20461 cttgcttctt ttttctttcg gatagataaa cgcttcaaat tttcttgcct cctctgtacc
    20521 tagacagtca aaaatggatt attgattttg cttgactaag ttttctactg ctttttgtgc
    20581 atctgccaat ccttcttttg gtgattttgt tcctaaaaga atctggtcaa tctgttcatt
    20641 cactaatgtc gtgtaatccg gagcatctaa aggaactggt gtgatcaccg tatttttaaa
    20701 gttttcgtcc gccatttgat agatcatttt tcctttatcc gttaggttgt cattattcac
    20761 taaattctca ttggctgtct tgctcgccat gatatcaaaa cttttttcgc cgaatttttc
    20821 ttgtacttct ggagaagaaa gatatttgat gaattcataa gaggcctcag gatcttttgc
    20881 tccgtatggt acttccaaga cgaatccgcc accccacgac caatggcctg aaccttcttc
    20941 tttttctggg agctgcatca cgccaaaatc aaaatcatcc ggtgcatttt ccatcaaact
    21001 agagtaataa ttgatatttt gggcacgcat ggctactaat ccggaaataa atggatcaga
    21061 tacaccagac ccaaattcgg cttctaattt attgatcgta tttttcccat aataatcttg
    21121 ccagtcttgg atccactcca aggcttccac tttattttta gtatcaatct tgactttttc
    21181 atctttgtca aaccaactag tcccgttatc tgcgtttagc gcccatacat cagcccctaa
    21241 attccataat ggataaaaac cgatgcgtgt aaattctttt ccttcttttt tatctagttt
    21301 acgtgctgtt tcttctaatt catcccaagt tgtcggtaga tcttgttcac tgattccagc
    21361 ttctttaaat aactttttat tataaaaaat gacttgtgta tctgtattga atgggatcgc
    21421 ataaggatca ccttcatatt ctactgtatc ccaaagctgc ggataaaaat ccttagaagt
    21481 gtctttttct acatatttgg agagattagt tgcttgctgt gcttctgctc tttgggcaac
    21541 ggaatttatg tcctgtacaa taacatcggg tgcatttcca gctgttattg cagataaaga
    21601 tttagtccag atgtcacccc atggctgata cttataattt acatggatct cgttttgtga
    21661 ttgattgaaa tcctcaatga tttcttcgat agcttctttt cgtgcaccgg atccccaaaa
    21721 ggaccagaaa tcaatatttt ttcttccatc acttgttttt tcagaagaat ttgctgcact
    21781 tgaactggca tcgcttgcat tacttgatcc gcatcctcct aataaaaaca atgctgaact
    21841 tgccagcaac cctgtaaaca ataatttttt catattttac ctccaaattt taatgtaacc
    21901 gatgccggtt ttaatataac gatatcattt tgttctgaaa ataacaattt ataaggtatg
    21961 attctaaaaa aattattaat gataccaata ctttatataa ttcgcgagcg ctttctttga
    22021 attttttaat ctttcgtagc agaatgagaa tgtattaaaa atagattagg aggaattttg
    22081 atgtttagac accagaaaga attgcagttt gaagcaaaac cagatcgtcc gaatccatta
    22141 atcgcgaaat atctgcagga gctgatcggc ggccaatatg gcgagatgag tgtagctatg
    22201 cagtacctgt tccaaggctg gagctgccga ggagaagaaa aatataaaga tatgctgatg
    22261 gacattgcaa cagaagaaat cggccacgtg gaaatgttgg cgacaatggt tgctcgttta
    22321 ttagagggtg cgcctgttga agatcaagaa gcagcgatgc aaaaagatcc aacaatcggt
    22381 gccattatct ctggaatgaa tccacagcac gcgatcgtag ctggcttagg acctcgtcct
    22441 gctgacagcg tgggcaatcc atggagcggt gcctatatca ttgcgagtgg caacttgtta
    22501 gcagacttta gagctaattt aaatgccgaa tcacaaggaa gacttcaagt agcacgtatt
    22561 tacgaaatga tcgatgatcg tggcgtaaaa gatatgctgt ctgttctcct tgcaagagat
    22621 agctatcatc aaaatctatg ggaatgtgca gtgaaagaat tagaagaaaa agaaggttca
    22681 atcttagtcc caagtacttt cccgcgtgac aaagaacgaa aagacattgc ttatgacttc
    22741 tataacttct cagagggaga ccaaagtagt gaaggttctt gggctagtgg caaagcactg
    22801 gatggcgaag gcgaatatca atggttgaaa gaacctaaga tcgaaggcgc tgcaccaaaa
    22861 ttagaaccag taacaccaaa aatgtatggg acacctccat tcaagaaata aatatctacc
    22921 cgtagaaaca cccgtttttt gttccacacg ctttgtgagc aaaactgact aaagtcatta
    22981 atcataacca cccttcaaaa gggtggtttt ctctagagct ataagctcaa atcaccggcc
    23041 agcgcctaaa gacgctggct ttcatatttt atgttcaagc catattgcct ttgccagttt
    23101 tgcccacctc ttaaaaggtt tatgtactac tccttagccc ttaaaaggat cgtcttactc
    23161 tcgtacactc agcttatcta acgcactatc atgtttttct tgttcttgaa tgtacttttt
    23221 gatggtcgct tcattttgtc ctacttttgt cacatagtat ccttccaccc aaaagtgtcg
    23281 atacccaaat ttatatttta gatttgcaca atctcttgat gatctcctga atattggctc
    23341 gatattgatt ataaattatt tttcttctat actttggtgt gaatacaatg tggtatttac
    23401 acatctactt tgtacgggct aaactatttc cctttattgc tccacccgca tagcaagtgg
    23461 ttttgtattt caatcgctcg gcgcgctcaa ttaggtctaa agacaataaa tctaaaaacc
    23521 cgctgcttca ctgacagaaa caacgggttt tcagaaaaaa atgaaatgaa aaagatctct
    23581 tacggcaatc tattttattt ttttgatttt ttataaatgg aaaatgcttc aagaagtcca
    23641 agaaataaag ccgtataaaa acaagaacct atgaaatctt tatttattag atattcaaca
    23701 gaaatcccga taacactagc caacaatata tttatcagaa gtgtaaaata aggatgcttt
    23761 ttatagaata gaactagtgt tgttttcata gactctcctt tcttcatatt tccaattaat
    23821 cacaaattca cggcattact agaaaaatta aaggggcgca gaagtggaaa aatactcttc
    23881 gactttccct acaaaccagg ccatcttgtg tttaataact ataccaaatc taaggtacct
    23941 ctaaatctaa attccatttg tggcggtgac ctccaatttt taactcttct atctcccatc
    24001 cagtatacat aaccttgata cttcgaccac ttatatatcg aacacacaaa tacctatcca
    24061 ttgcaggagt actacctaac ttagttgcta cttgcatcta aataacagct gaaaagatag
    24121 gacttactct tagtaaagta tctgaactat taatttttaa actatcattt ttatcctcaa
    24181 ctacatgcac atctgttgca acttcacttt ctgtctcatc cagataaatg attaaggaat
    24241 cgggaattgc aatatcttca gctgacgctg gaatagttga gctaaattag tcatcatatc
    24301 tttattataa aataaccaac agcaaaaaat acaatgaaaa acaaacttgg aactaatgta
    24361 tcattaataa atgttgaaaa attcagcaac tcattactgt atgattttag cagcccaacc
    24421 aacatatacc ctataaaagc taataagatg gttaggaatc ctttagaatt tttactcatt
    24481 catagacact tccttaaata acttttatta tagaaggtat tgtaaataat aatattgaca
    24541 aaaagtgact caaaataaaa aaaaccttac attatactgg aatgaactgt attgatagtg
    24601 tacctaaaag ctctactgac ataagatcgt gtaatcggta aagtaatttt attaattata
    24661 cgggatgtaa tcaattagag aataaactaa tcataaaagg tacttttaca ttcataattg
    24721 tcgctttgag attctacttt ggttcactat ctgacccaat tgcacatttt cgatcattac
    24781 aagtattggt gtcgttagaa caactaaata aaaactagcg gtagattttt cagaatctat
    24841 gctaatttct gtatggatgt tccagtgatg gcaaatttct cttttcattg tttttcttgt
    24901 cgcatgatat gcctttttgt gttattcagt taaactatag aaattctttc ctagacagat
    24961 cttgatccca aattttacgt ttttgagcat cgatatagtt taaaatgaca ttactcatat
    25021 ctaaagagtt tagatttttt aggactaaaa ctgtcagttg ttctttttct tctttctgtt
    25081 aaagtattgt cgcgactttt cgtatacgtt aagatatgtc gtgaaaaaac aagttttgtg
    25141 acacaaaaaa acgaatgact tgaggaaatc acctcaatca ttcgtttaaa atgctatata
    25201 ttctttagtt taagatacgg aaattaatgc catcgaccac actagtagaa tgaacgacat
    25261 ttacttggcc tttttttaat ttcgttatgt ttagcatata aaaatattat agagtttact
    25321 tacaatgctt aaaacatata ctgtataatc tgatcaattt tagccatgtc ttgcaatggt
    25381 aagttttcta tttttttgag ttttcgttcc ttaaaatcaa gagattttaa ttgtgtaata
    25441 attacttgtc cttttatatc tagctcttca ggtaatgagt aacgtgtcgg taaattcttt
    25501 atagtggaag taattggaca tatgaccgaa aacattgttt tacggttaaa ttcataacga
    25561 gagacgacta gtcctggtcg tcttttttga atttcttttc ctacggaggg atcaaaatca
    25621 atccatacaa tatcaccttt tttaggaata taaattcttt cttctgacac tataaaattt
    25681 ctctcccttc aggagttaaa tcttcccatt cgtctttctc ataatactcg gcctcttccc
    25741 caccactaaa tggatcttca atttttggaa ctaacgtaat tgttccatca tcagaataca
    25801 caacgatata ttcttggttt tctgaaggtt tctttccgtt atcagaaggt aaagttacca
    25861 ctacagaact tccttgtaat ctagattttg taacaagcat tatcatcact cccttaataa
    25921 aagtatacta tataacacta agtaacgcaa aattcccctt tgtaaactat ctagaaaatt
    25981 gttttgttta aagtatatct tattggatat aaatagaaat aaattgtagc cagttcattt
    26041 tttctttaac taaaaaaata atccaagaaa cattgaacct atggatccaa aagctaccca
    26101 aatactatag ctaacccccc gctaccttca attttgcaga taaaacgctt tgggagaaaa
    26161 tttcaaagac aacaggccat cctatcatgc ccgattataa acaaaaagct ggctattcct
    26221 tttgctctga caaatttcaa ttgggaaagt tcacgaaact agcaggtgaa gaagtttcaa
    26281 ctgttcggat caaagaatgc ccgcttcaaa tagaaacatt ggttcaacga atttcaacta
    26341 gacaagactt tgctattgta gaacgtgaga tcgtagctat ttttgtagaa gaagggattt
    26401 tacatgatcc aactcatatc aatgtggata aatggcaacc tcttatctat aaattcagag
    26461 aatatacaac tgccaaccag tcgttaactt tgaattttag attccaagaa ttcaaagagt
    26521 cataatgata aacatcgtta ggtataaata aaaaagactc gcagcacgag taaaaaacaa
    26581 atagaagcta tacattagaa aggtcgactg tgaccctata gatgcagtat atgcagctta
    26641 tgagcatttc ttctgttctc tttgtttttg ggatctgtgc tgaagtcttt ttcgtttttt
    26701 gccgctcttt ctttgaaaaa tcgattatgt tataccttct ccattactaa ttcaggcatc
    26761 tgctgagtaa tgatttttcg cgcaaaagaa atatctgtta cccaaggttc gatttgttct
    26821 tcagtcaaga caagcggcat tcggtcatgg attggtgcga ctgtgcggtt aggttcagta
    26881 gtcattatga ttgattctgc cattttttca tcctgatggc tacgataaaa accgccaata
    26941 tacactattt tttccttatt tgaaaacata aatttgtctt tttcttcatt ccactcattg
    27001 ggaaaacaca acgattttgc aaaaatggtt gatgaaaggt ctttttttct tctactgtct
    27061 ctgcacgggc attaatcatc aattgtcctt ttttaaatcc ggtaaatccc cacttcatga
    27121 tttccggagt gaggatgttt tcttcctctt ttcttaacgt cacaacaaga tttgacggaa
    27181 aaacttctcc agctgctaat tctgctgcct gcttcggttg tttcgtctgg gcttctcgat
    27241 aataattctg aaggagctct gactctagat caaaaaaata acgaccacac atcctaatcc
    27301 ctcccaatct ttttttctat catacactgc aacaagaagt tcgcaccaac aaaacgcttt
    27361 caatttttgt agaactaaaa aagttgtgac aagtgacaaa ctctccagtg aatggtattc
    27421 tcctgccaac tcctcggtca caagtcacaa tattcaacaa acatgagaag ctgctttttg
    27481 aaattgttcc ttgtgactcg gagtgaaacg gcaggcttac gacctctggg taaacggtgt
    27541 tcaaaagcca gtttctcggt attaagccaa atttatgcaa aaatgtagaa agctattttc
    27601 acaaaatttt tcttaatact cgaaactaag cgactttttc acaacctctt ccttaaacgg
    27661 tgttcaaaag cttgcatctg cggtaataag cccaaccaaa cgaaaaatat gagcagctat
    27721 ttttatttgg ctgttcttat tacttgatgc agggcagctt tttcacaacc tctccactaa
    27781 acggtgttca atcagctaac cttcggtatt agctcaaaaa acggcaaaat atgaggaact
    27841 attttgccgt ttgtttctct aatactcagg tcaaagcgct gatttcacaa cctctaatac
    27901 acggtgttcc agaagtagct tcttcgaaaa ataagccgaa atttcacaaa aatttgagaa
    27961 gcaattttcg taaattcctt cttatttctt gaagctgacc acttctgtca caacctctaa
    28021 ttattaaaga accttttgcg aagttaacaa tctttctttg atcaattgaa aattctgcct
    28081 aagtagtgat agatcctgtg tgactttcaa caataagata cttccctgga tttcagcaat
    28141 caacgctttg gcttgacggt ctgcttcttc atcggacagt ttttcttcag aaagtgccag
    28201 gatattttgt ttcgtatact tgacccattg attgactaat tctagtagca acagacggta
    28261 ttcttcatcc actgtactca actcaataat caaattacct acaggacagc catgataagt
    28321 gatctgtgtc tcatgatcag cgaaaatcca gtccaacatt tcttctaatg cttcttctgg
    28381 ctgttttcca gcatttaaga tgtcttcgaa caattctgtc tgccatacgt ccatatgcgc
    28441 atggatcacc gctagacaaa cttctttttt tgaagaaaag taataataaa attgaccttt
    28501 tcctgtatca gaagctgcta gaatatctcg gattgacgtt tcatgatagc cttttttata
    28561 aataagatct ttggtcgctt ccaagatctt ttcctttgtt ttcaatgata ttctcctttt
    28621 tcatttctca tagtcctata ttatacagta aattcatcat tttttcttct taatcgttta
    28681 aaaaagactg tgccaaatta tctgacacag ccctcactat aaaactgttc tcatatagct
    28741 agatgttcat tgattcaaga actgcgagga caaaagcaat tcctatctta tgatagttgc
    28801 tctaatactt cctctgttgt catcacttga tctgcaataa agccaaagtt cgtcaacgct
    28861 gcatccattg cttcttggcc aggagcacca attgcatctg tcgctaccac aacattgaat
    28921 ccactctcta ccaattcacg catatgggaa tcgctacaca agtttccgtt cattccacca
    28981 agaattactg tgtcgattcc attttttctc aattggagcg ccaaatcatt gctttctggt
    29041 ccaaagattt tatgaggaga gctgatgatc gtattttcat ccaaataagg tttcaattct
    29101 tcgataaaat cggctcctga accttcttcc actgattcat attgactttt tctagcaaac
    29161 atctgctgct caagcatcat atgttctcct gcaccgccaa attgccattt atggtcatgt
    29221 gggtaaaaat aatgaggaga aataaagagt ttgtatcctt tttcttttgc ttgttttacc
    29281 atttcaatca tattttcgat cgtattgtat ttttctaaga tatcttttgt caaaccaaag
    29341 ccaccgcctg ctggtttcaa gaactcgact tgcgggtcag tgagaactac tgctgtattt
    29401 ttattattga acatggtgga ttctccattc gttatttatt ttaatacatc atttagagct
    29461 tcactcattg tcggatgaga atagatttga tctctcaata acgtataagg aagttttcca
    29521 ttcatagcta gcgaaatcaa gttgatgact tcatgagaat cttccgcata gatacttgcc
    29581 cctagaatca agtcagtttc agggtcgact aaaactttga atacaccttt aggatcttct
    29641 aatacttgtg ctttagggac accgcttgtc atatacttga ataatttgta attgacgcct
    29701 tgtgctttgg cttgtttttc tgttaaacct acattggata aagtcggagt gataaataca
    29761 ctgtaaggca cagctttccg gttattcgtt gttcgtttat tctcgccctt caactgatcc
    29821 ataatgattc gataatcatc tagagagata taagtgaact gcaatccgcc tttgacgtct
    29881 ccaatcgccc aaatgttgtc tgcatctgta cgaaggtatt catccacagc gattgctccg
    29941 cgatctgtta gtttaacatc tgtattttct aatccaagtg atgctgtact tggttttctt
    30001 ccagttgcag ccaaaacttt tgacgcttgg attgttactt cttggccatc ttctgtaaag
    30061 gtgacggcag ctgttgattc ttggtctgct acttgatcta cagatacacc tagatggaat
    30121 tcgattcccg catttgtcat gtcttctaaa atcatttgcg cgatatcttc atcttcacgt
    30181 ggaagaaatt cagaatgggc atcaagaacg acaactttag atccgtattc attgaacatt
    30241 gatgcaaatt ccaagccaat atatccagcc ccgatgatca ctaatttttc cggtaattgt
    30301 tcctgatcca tcgcttgcgt agaatcaatc aagtagcgac ttgtattcaa tcctttgatt
    30361 ggtaaaatga ttggttcaga accagtattg atgaaaatcc gttcgccttc aacaatcact
    30421 ttttcaccat tattcgtcac ttcgatttga tggttagaaa gaaaacgtgc tgtaccgtcc
    30481 atcactgtcc cagttgcctc atcgctgatc atatgataat ttttggcgcg aagcatgccc
    30541 gtcaattttt ctttccgagc aacagcctct gtaaagtcga ttcctctttc tccattgaaa
    30601 atcaatgatt tagaaggaat acagccgatg ttgatacatg ttccgccata catacgtgtt
    30661 gacttttcaa cgactaaaac cgattcacct tttgaagcta acgccttcgc taaagtctta
    30721 ccgccctttc caaaaccgat cacaatattt tgatattttt ccatcatttt tccctcgctt
    30781 cttttttacc taccgattgg tacaaaaata tgataaccga taattgtcta ttctccaaat
    30841 catctgcttt atttgtca
//
LOCUS       DANKEF010000031        27335 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000031 DANKEF010000000
VERSION     DANKEF010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27335
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.031"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1143
                     /locus_tag="PWZ48_001580"
     CDS             <1..1143
                     /locus_tag="PWZ48_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDL2261239.1"
                     /translation="NHEVKTIDADKKTVTVTSASDNETKEYPYDKLILSSGVKPNNLP
                     VPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVGAGYIGIEAAEASRKAGKEVVL
                     LDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKIEAFTGNGKVEAIKTSEKEIPA
                     DLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTNLPDVYAVGDATLAYSIPARKK
                     MPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSALSVFDYHFAASGLNSFTAAKA
                     GVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMTHQLLGGAVLSTYDVTGQGNVL
                     ALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQALGEEPFVE"
     gene            1424..2221
                     /locus_tag="PWZ48_001581"
                     /pseudo
     CDS             1424..2221
                     /locus_tag="PWZ48_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2410..3078
                     /locus_tag="PWZ48_001582"
     CDS             2410..3078
                     /locus_tag="PWZ48_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDL2261240.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(3278..3508)
                     /locus_tag="PWZ48_001583"
     CDS             complement(3278..3508)
                     /locus_tag="PWZ48_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261241.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(3635..5017)
                     /locus_tag="PWZ48_001584"
     CDS             complement(3635..5017)
                     /locus_tag="PWZ48_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2261242.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(5173..6360)
                     /locus_tag="PWZ48_001585"
     CDS             complement(5173..6360)
                     /locus_tag="PWZ48_001585"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDL2261243.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(6367..7398)
                     /locus_tag="PWZ48_001586"
     CDS             complement(6367..7398)
                     /locus_tag="PWZ48_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HDL2261244.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(7444..8868)
                     /locus_tag="PWZ48_001587"
     CDS             complement(7444..8868)
                     /locus_tag="PWZ48_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2261245.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMED"
     gene            9043..9948
                     /locus_tag="PWZ48_001588"
     CDS             9043..9948
                     /locus_tag="PWZ48_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDL2261246.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            10039..10716
                     /locus_tag="PWZ48_001589"
     CDS             10039..10716
                     /locus_tag="PWZ48_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2261247.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            10713..10883
                     /locus_tag="PWZ48_001590"
     CDS             10713..10883
                     /locus_tag="PWZ48_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261248.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            10997..11893
                     /locus_tag="PWZ48_001591"
     CDS             10997..11893
                     /locus_tag="PWZ48_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2261249.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(11948..13318)
                     /locus_tag="PWZ48_001592"
     CDS             complement(11948..13318)
                     /locus_tag="PWZ48_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2261250.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            13556..14302
                     /locus_tag="PWZ48_001593"
     CDS             13556..14302
                     /locus_tag="PWZ48_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2261251.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            14299..15138
                     /locus_tag="PWZ48_001594"
     CDS             14299..15138
                     /locus_tag="PWZ48_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2261252.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            15139..16089
                     /locus_tag="PWZ48_001595"
     CDS             15139..16089
                     /locus_tag="PWZ48_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HDL2261253.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            16230..16982
                     /locus_tag="PWZ48_001596"
     CDS             16230..16982
                     /locus_tag="PWZ48_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261254.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            17001..18152
                     /gene="queG"
                     /locus_tag="PWZ48_001597"
     CDS             17001..18152
                     /gene="queG"
                     /locus_tag="PWZ48_001597"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDL2261255.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            18536..19144
                     /locus_tag="PWZ48_001598"
     CDS             18536..19144
                     /locus_tag="PWZ48_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2261256.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            19312..20949
                     /locus_tag="PWZ48_001599"
     CDS             19312..20949
                     /locus_tag="PWZ48_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDL2261257.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(21021..22133)
                     /locus_tag="PWZ48_001600"
     CDS             complement(21021..22133)
                     /locus_tag="PWZ48_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDL2261258.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(22208..23581)
                     /locus_tag="PWZ48_001601"
     CDS             complement(22208..23581)
                     /locus_tag="PWZ48_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2261259.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            23802..24419
                     /locus_tag="PWZ48_001602"
     CDS             23802..24419
                     /locus_tag="PWZ48_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HDL2261260.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            24517..24972
                     /gene="argR"
                     /locus_tag="PWZ48_001603"
     CDS             24517..24972
                     /gene="argR"
                     /locus_tag="PWZ48_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HDL2261261.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            24990..26492
                     /locus_tag="PWZ48_001604"
     CDS             24990..26492
                     /locus_tag="PWZ48_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HDL2261262.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            26587..26793
                     /locus_tag="PWZ48_001605"
     CDS             26587..26793
                     /locus_tag="PWZ48_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261263.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            26879..27280
                     /locus_tag="PWZ48_001606"
     CDS             26879..27280
                     /locus_tag="PWZ48_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2261264.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
BASE COUNT         9122 a         5068 c         5564 g         7581 t
ORIGIN      
        1 aatcacgaag taaaaacgat cgatgccgat aaaaaaacag taacggttac tagcgcatca
       61 gataacgaaa caaaagaata tccttatgac aaattgattt tgagttctgg tgtcaaacca
      121 aacaatctgc cagtaccagg agcagatcta gaaaatgtct acttgatgcg tggatacaat
      181 tgggcaacca aaatcaaaga aaaattaaat gatccggcta ttaaaaaaat cgctgtcgta
      241 ggtgcgggtt acattgggat cgaagcagca gaagcaagcc gaaaagctgg gaaagaagtc
      301 gtattgcttg atgtgatcga tagaccttta ggcacttatt tagatgctga gatgacagat
      361 attttagaac aacacctaaa tgaaaacggt attgaagttt tgacaaatgc aaaaatcgaa
      421 gcttttactg gtaacggaaa agttgaggca atcaaaacaa gtgaaaaaga aatcccagca
      481 gatctagtca ttcaagctgc cggtgtgaag gcaaatacag aatggttaaa agggatcgtc
      541 gacttagatg aacgtggctg gatccaaaca aatgaatact tgcaaacgaa tcttccagat
      601 gtgtatgcag taggagatgc tacacttgct tattctatcc cagcacgcaa aaaaatgccg
      661 atcgctttag cgactgttgc acgtagagaa gcacgttatg tagtaaaaca tctttttgaa
      721 gaagtaccaa acaaaccgtt cggcggagtc gtaggttcct ctgcattaag tgtctttgac
      781 tatcattttg cagcaagcgg attgaatagc tttacagcag caaaagcagg agtagatgta
      841 cgagtgtctt attatgaaga tacgttacga ccaaaatatg taccagaaga acaaggaaat
      901 ccaaaagtgt ccatccaatt attctcggat ccaatgaccc atcaattgct aggaggagca
      961 gtattatcta cctatgatgt tacaggtcaa ggaaatgtat tggcactagc gatccaaaac
     1021 aaactgactt tagaagaact agcagaagct gacttcttct tccagccagg ctttgaccga
     1081 caatggagca tgttgaatct agccgctcaa caagcgttag gagaagaacc atttgtagaa
     1141 taagaaaacg aaatatttga aaagaggttg tgaaaaagct gccttgcatc aagtaataag
     1201 aacagccaag taaaaacagc ttcttatgtt tttaacttgg ctggacttat taccgcagat
     1261 gcaagctttt gaacaccgtt tattttcaaa aaagggactg tgacacgatt tgtgtaccga
     1321 cccccaaaag ttagaccaaa aaatctaact tttggaggtc ggtatttttt atggttaaat
     1381 atacttttga acttaaatta aagatcgttc atgattattt ggatggcaaa ggcgattgga
     1441 ccgtaaaaat ccagatcaag agattgaagc ggaaatgctt aaaattcggg agaagcacaa
     1501 ggattatggc tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa
     1561 aaagaaagtt cagcgcttga tcaagaagct aggaataaaa gtaacggcat acacgcgtaa
     1621 atcacgtcgc tacaattctt atcgaggaaa agttggagca gtagccaaaa atcggatcca
     1681 tcgtcgtttc tacaccagta tctgccatca gaaaatcacg acggatacaa ctgaatttaa
     1741 ataccatgaa gtggatgcca aaggcattat ccgtcaaaag aaattgtatc tagatccttt
     1801 tatggatatg ttcaatagtg agattttatc ctatcgtatc tctgaaaagc ccaatgcatt
     1861 agcggtaatg gaaggtttgg aggaagctat ccagaccact aacgattgtc cctatcgtcg
     1921 tacgtttcac tcagatcaag gttgggcgta tcaaatgaaa gcttatagaa acaaattgaa
     1981 ggagtacaaa atctttcaaa gcatgtctcg gaaagggaac tgcttagata attcacttat
     2041 ggagaatttt tttggcttat taaaacaaga gatttttcac gggaaagttt ataactgttt
     2101 cgaggaatta aaatcagcca tcgatagcta tatctattac tacaataatg aacggataaa
     2161 acagaaatta aactggcaaa gtctcgtcca atttcgtaaa acaaccgtaa cggttgtgta
     2221 aaaatatcat tttagagcat aaaaaaaagg agtggatttc tccactccaa aaaagtctaa
     2281 cttttggggg tcactacagt gtcacagtcc tttttttagt aaaaggatac atttcttcct
     2341 tgtatattcc atgcgtgttc gtgagaaagc atatataatt taattaacag atagttaggg
     2401 ggagaaaaaa tgattttttc aattacaatg aaactggtgt tcgggttgat cggtttatta
     2461 ttagtggtac gtttgcttgg gaagaaatcc atgtcagaaa tcacaccgtt tgatttagtc
     2521 tatacgcttg ttttaggtgg gatcttagaa gaatcaacct atgatgacaa tgtccatgta
     2581 gggcatgtcc tatttgccat tgcgctttgg gcagtaatga tttatggtat cgaacgtatc
     2641 gtccaaaaaa atgaaaaggt caatcgttgg gtcaaaggcg agccatctgt tctaatcaaa
     2701 gacggcgtca tcaacatgac agagttgaca aataaccata tcgagatgga gcagttgcga
     2761 gcaatcttaa gacagcaaga atgctttttt cttgaaaatg ccaaacatgt tatcctagag
     2821 aatgccggtc aaatgagtgt attgaaaaaa tcagatgaag acaaagcttt atcgatcctg
     2881 cttgtagatg aagggcaaat tcagcataaa gtgcttcagt ccaatcaatt gactgaagca
     2941 tggcttatgg agaacttgaa gaaagaaggc tatgcagatg taaaacagct gatttatgtt
     3001 gaatggtcag aagaaaaagg gctctacata ctaaataaag aagacgtcat gcatgaatct
     3061 taccgaatag atggatagat agacagaggt tgtgaaatca gcgccttggc ttgttactca
     3121 aaactaagcg actttttcac aacctctgat atctaatata cgttgttcca gaagttgctt
     3181 cttcgaaaaa taagccaaaa ttccacaaaa atttaagaag caattttcga aaaatccttt
     3241 cttatttttt gaagttgatc acttctgtca caacctctca tgtcacaaac catttttttg
     3301 tttcctttaa ctcaatatac cgtacgtctt cccacaatat cggctgatgg tcaatcataa
     3361 agtaatcttc ataggcttca ccaatgaagt gcccttcgac ctcatccaag agtcttccaa
     3421 attcatcttt taaattgagc tgaatgatta ctgctttacg agataaaaag gcttctgtca
     3481 gtatactatc gatttcttgc aatgtcattt gttgtttagg aagaggctgt ttgcgtgctt
     3541 cttcttcatt tttttgatac cgttgactag ttcttccatc gcaaaagcag tcccccattt
     3601 taatccaaat ggccgatctc gatactcgtt atagtcaatg aatttttttg ctgtccgtct
     3661 catcttttcc accttctaat ccactcatac cacctgcatg cccgccaacg agagaactac
     3721 gtgcgatggc gcgtcctcct tgcaagagac tatgcgcatg gatgattgat tgaaagccgt
     3781 atttttttct aatcgtatcg acaacaaaat caactttcgc ttcatttatc tgcttttcag
     3841 gatcttcaaa aaggttaagc tgtaaacctg tcgcgtatac gaggtcggaa cagttcacgc
     3901 ctacacgccg aatatcctgg tagtggtaat gctggtggaa aatggtcaat aaagcatctg
     3961 caagatctct gctagcattt gtcgcttcga ttttcatttg ctggtgaaac ccggtcttgc
     4021 catttggatc aagatagcct aaagagtatc ctacccataa gctgactact tgtgtttttg
     4081 ctgcagatcg tctcaagcga gtgccgactt ggtcggccat ttctttgata acgatctcga
     4141 tttcttctcg tcttgtataa tctctatcta ggatttgact atttccgata ctttttgatt
     4201 ttggtgtata ttcttgtccc aaaaaacttc gatcgatgcc ccaagcatgg gcataaagct
     4261 gagtaccgat gacccctagc ttgcttttca agtgataata atccgcgtga gctaaatcat
     4321 aaacagaatg tatacccatt tgtttcagac gttttgctgt tcgtttgccg attccccaaa
     4381 agtcagtgat atctgagatt ttccataact tttctggtac atcttcataa cgccattcag
     4441 caaacatttg ctttccgtct tttgagccat tgtctaaggc tagtttagca agtagcgggt
     4501 tatcgccaat tcctatagtc gtgtagattc cggtctgctc aaacacatct ttatggatca
     4561 gtttagccat gtcgtacgca tttgttttac caaagagtgc catgctattt gttacatcaa
     4621 ggaagctttc atcgacggaa tagacatgat ggttcgcttc gtctgcatat tttttataga
     4681 tagaattgat ttctctgttc ttttccatat aataggccat ccttggggca acaatatata
     4741 aatcacttgg ataggggaag ggaagatctc tggcacggct gatattggtg atgccgtaag
     4801 ctttttttgc tgcaggactg gaagccaaga ttaatccgct accccgtgtt tctgtcgaat
     4861 cggacggata ggacatgact actaatttag ttgtaagggg atcaagatcc ttttcaacgc
     4921 actctacgga ggcgtaaaaa gatttgcagt caatacataa aatatctctt gaaggttctt
     4981 tttcataaac aaataccgga ttttgattcg cccacataac aatcacctct tcaacacaat
     5041 tagaacacac gttctctttc cgtgtcaata aaaaacagca attttcaaat acgagcaaaa
     5101 ggctcacatt tggaaattgc tgagaggtta tttttatcta tttgttttta gctgagatcg
     5161 ttgatttttt tatcaaacat gaacagcttc tggatcgatc tttgttgtcc catttccaat
     5221 gtgtgcaaca taaaattccg gagcatagcc gacaactttc atgtattcat catagacatt
     5281 gtttttgaag gcaggtatat tttcttcttt gacaagagca atcgcacagc caccaaatcc
     5341 agctcctgtc attcgtgcac ctaagacacc ttcttgtttt tgagcagcat ctactagtgt
     5401 atctaattcg atcccagtta cttcataatc atagcgaaga gagtggtgag aatcatttaa
     5461 cagttgacca aatgcttcta agttcccagc ttctaattca gcttttgcct tcaaagtccg
     5521 ctgattttca gaaacggcat ggcgagctct gcgtatcaat gtatcatcgc cgatttggtc
     5581 aatagttgat tcaaattctt cttcatctaa gtctcctaaa gaagagatag gcaaagtttt
     5641 ttgcaaacgt gccaaggctt cttcgcattc gctacggcgt tcattgtatt ttgaatctgc
     5701 caattcccgc cgcttattcg tattcatgat gacgattgca taaccatcta gtttcactgg
     5761 aaccatttcg tatttcagcg tatttgtatc taataagatt gcatgatcta gctcaccgaa
     5821 gccgatggca aactgatcca tgatacctga attgacaccg atgaattcat tttctacttt
     5881 tttacctgtt tggactagtt ccaagcgatc tgtttttaaa tcaaacagat cttctaatac
     5941 aacacctact aataattcta atgaagcgct actggaaagt cctgcaccat tagggattgt
     6001 tccttccact aataactcaa acccatgatc gattgagtgt ccagcttctt ttaatttaag
     6061 gaccattcct tttacgtaat ttgcccagtt tgctttttta tcatattcta gctcatctaa
     6121 agtaaaacta atgacaccga tatcgtcaaa gtttgtggaa taaactaata ttttattatc
     6181 gtctcgaggt gctgctacac cttgcgttcc ataagtgatg gaagcgggga agacatggcc
     6241 tccattgtaa tctgtgtgtt caccaatcaa attgatgcgt cctggtgaga agtattgtgt
     6301 agttttttta ggtccgaatg tttcggcaaa ttttgtgttt aagcttcttt tgatttccat
     6361 gatgagttat tcctttcttg ttttgatttt gaattctgtt actgtttcaa atactgatcc
     6421 ggcttttaaa tgaatcgaac caaatgctga gaagcgctct gcacctggag ctgtttgagt
     6481 ctcaaatgtg atgccaccgt gatgtgccag ttttcggttg cgcatttctg gtgtttctgt
     6541 gccgaaattt gcagtgaaaa taaccacact agaagcatct gtagcaatat ctacttggat
     6601 tcttttatcg gggctagtca agcgagcggc ttctttaccc aagccggttt cttttaagaa
     6661 aaatggatga tcgattccgt caacaagatt tttttgatcc aaatcactag caaaaacgga
     6721 cgctaatttt ttaggtattt gaaaatcgaa agcacttcct gcagcatttt cttttacccc
     6781 tataggaata ctatctgttc gcaaaggagc ataagcttcg ctattgagcc aaagctcatg
     6841 ttgatcgatt gactgagaag catctcccgt caagttgaag taaacatgat ttgtcggatt
     6901 gaataaagta tcttggtcac tggtgccgcg agaggtcact cgccagatat tatcttttgt
     6961 caaagtatag cggatttcca catccattgt acctggaaaa ccatgttctc cgtcgggact
     7021 tgtagtagta aaaatgacag aagcttcatt ttcgccattc aaaatgacat aagaccattt
     7081 ttttaattca aatccaggag caccaccatg cagattatgt ccagtttttg gatctgtggc
     7141 taattggtaa gtttttccat tgagagaaaa gcggccattt tcgattctcc ctgcggtgcg
     7201 accgatagaa gcaccgatat acggatcttt ttcgatatat tcttcagcgg tgtcaaatcc
     7261 caaaactaat tctcgatcat ttgattttaa actgacgatt ctagcaccta aatcagagat
     7321 agaaagtgct agcttgtttt tgttctccaa ggttatcaag tgataacctt ggccaaaatc
     7381 tttttctgta atcttcattt tcaagatcct ttcttaatgg gaaggaacta ctgcatcgtg
     7441 aagttaatct tccattgtat cagctggagc caatttgttt tccaatttct caacgatttg
     7501 tgcatgtttt ttctctgtca atgtaatctt ccaagcaaaa ataatagcgg agaaaatcat
     7561 caaagcagca ggagcataga atgcataagc tttgaaagtc gttacgccat gagcagtgat
     7621 atcagcagca gtagcattac cagtcatccc agaagcgata gcgacaaacc ctacgatccc
     7681 gttagacaaa gcaccagcga ttttatctag taaagggcga attgatagag taacagcttc
     7741 gttacggata ccatttttcc attgaccata ttcaacagag tctgtgatcg tcatcaaagc
     7801 ggataagaaa atcaattgtt gagggaagta gaacaatact agcccgatga taacgattgg
     7861 caaacttgaa cctgcgatcg taaagaaaaa gtaagaagaa atcatcattg cgatccctcc
     7921 aaggtaaacg tagcgacgag agatgacgcg tgtaagcatt gggaatagtg gaacagctac
     7981 taaaccaagt aaggcagcaa ctagaccaac gatccaaaat tcagttggtt ggccaagtac
     8041 atatttgaag aagtagaata atacggcagt tgttgctgca ttggcaatcg cataagcgat
     8101 ataagcaagg gcaagccaca tcaactgatc attttgagca actgctttga acacatcttt
     8161 gatcccagct ttttcatttt gctgacggat cgcagtatcg acttcttttg tgcccaaggc
     8221 agtgataatc gcagtaccgc ctgaaataac agcagcaatc acaccgaacc agaaccagcc
     8281 tgaagcgcct tcaatatgtt tatgagtaac aagataggta aagaaagtaa cgatagggac
     8341 aacgactaga gtagtaccat ttgcacctag agaagagccg aaacgagcaa acgtagcaaa
     8401 tttttcacgt tcctttgttt ccgtactcaa agctggaatc attgaccaaa aagcgatatc
     8461 cttaaatgaa taaaaactat ccaataagat gaaaacgatt gcaaataata tgataaataa
     8521 agtcgtatta tgagtagcta agccaaagaa attggtaaag attgctacca agagtacgga
     8581 actaacaacg ccgccgataa ctaaccatgg tttgaattta ccgtattttg tacgagtatt
     8641 atcgatgatg ccaccaatca aaggatcaaa aataatttca actaagcgga tacctacaac
     8701 taagcttgta actgtagcaa tcatttttgc attcgttgcc ttatctgttc ctgcgaacat
     8761 ctggcttgtc acgaatacca taaaataagt gcttaatgta acatagaatg tgtcgtgtcc
     8821 aaaagcgcct aaagcgtagg acacacgttg gatcattttg cttttcataa tctgtattcc
     8881 tctcctgttc aatatataat actaagtaaa ccggttacaa cgaataacaa agatctttta
     8941 atgacttaaa cttaacaaga aatcgcttcc tttttaatat atttctgtga cttttattta
     9001 actttttgtt gcattatact aaaggagtaa ggagggatag acgtggagca atcgattttt
     9061 ttaaaaaagg aacatcaaca agtagaaaaa gaattgtatt ttgatttttg tggtttctct
     9121 aaaacgatgc cgtttcattc ttttggtcct gctattcgca atgattacat cctgcatgtc
     9181 gtgatggaag gcaaaggatc ttatcatgtg aaaaatcaac aattccaatt gaaaaaaggt
     9241 gatctgttct tgattcgtcc aggagactcg acgttttact tatcagatgg agaagatccg
     9301 tggatgtatt gctggctttc atttggtggg agtgctgcag aagaaatcat tcggcagtcg
     9361 cttttcagag aagaccaata cacgatggtc tcttctgaca tacaaaaata tatcgacatt
     9421 attttagaat gcatgaaaga tacggaagaa accttggaaa atgaattaca gttaacagca
     9481 ttaacctacc gttttttatc agtcctttta aaagatggcg gaaaggtcag cggtggtgta
     9541 caaaaggttc attcctcttt agctgttgaa acagtcaaat acatcgaggc acattatgct
     9601 gaaaaattgt ctgttgagga aatcgcccag catttagcgg tcaatcggag ccatttgtcg
     9661 cgagtattca aaaatcatat gggtaccagt atcaaggaat atttgatcgg ggtacggatc
     9721 aatcgagggg cgtttcttct ttctctgacg gatgaatccg tggaaagtat tgcctatcaa
     9781 gtagggttca atagtctagt cgtatttagc cgaatgttca aaaagagtac cggagaaacg
     9841 gctacgaact atcgtaaacg tatgaaaaac gaaaatttta agaatattgc tttggatacc
     9901 ttgaaaaaac agttggaaaa acaaaccgca gtttcttgga caacctgatt agtctggtta
     9961 aaatgacaag catcggcaaa agaaacttgt ggcggttatg ctacactgta aagcgaagac
    10021 taaaaaggag gaaaagggat gcgtacactt atcatcatta gccatccaac gatcgaagaa
    10081 tctttaaatc agcaattttt caaagaagct tctgctggtt taccagctac ttggcatcat
    10141 ttggaatcaa gttatccaga cggaaaaata gatatagaga ctgaactaca gctcttggcc
    10201 cagcatgatc ggatcatttt ccagtttccg ttttactggt atagttctcc tgctcattta
    10261 aaaatttggc aagataatgt cttggaacga gcagcacatg tattaaaagg gaaagaacta
    10321 ggaatcgtat tgacaacagg tgtggcggaa aaagaatacc aagctggagg aaaagaagaa
    10381 tatacgattt ccgaattttt gaggccgtat cagcgaatcg caaataaatt ccatatgacc
    10441 tatcttccgc cattcgtttt agcacaattt atgtatttat cccaggaaaa acgttgggag
    10501 aagctaattg cttatcagca gtatctctca ttggaaggca aaccatcgct gactcagcgg
    10561 attgactggt ttattcaaag agtacaggaa aatcagaaaa tgcaggaaga agaatctgaa
    10621 aaacaagcat atatcgtaga agcactgact gaggcaaaag agcagatcga agacttgacc
    10681 tttacactgc aagaaatgaa aggaacttca ttatgacaaa tgattggctg atccaacaat
    10741 tagatgaatt aagcgaaaaa tcagaatatc aagaccgtgc gctcttatat gaaataaaga
    10801 aactgatggc tgagatagac gaacgaacaa tgcagctgca aggagaaatc gacggacaat
    10861 cttggaatca tctcaactgg tgaaattttc ttataaataa caaagataaa aatgtgcaca
    10921 gcgacaatta tttgctacaa taagaatatg aagcgtttga taacgctatt atacataaaa
    10981 attggtggtg aaaaaaatgt atgatgaaaa tgagtgggga gaaaatgttc ggatgacccg
    11041 aagaagacag caaaaacagg cagctcgtaa gcggatgatc atgattgggg cagcagttgc
    11101 cgtgtttttg atcatagcag gaggagttgc gggtctatat tttcatggaa aaaattcgtc
    11161 agaagcgaaa agtacgacca taacggaaac aacgacaaag aaaacagaga cgaaacaaac
    11221 aagtgagtca tcaactaaaa caaaagagac acaaacttct gaagaaaaaa cagcaaatgg
    11281 tttgccagag caacttcaag caatcgtaaa tgattattcg aaaaagatcg aagaaaagac
    11341 gcctgcattg attgaagaat accagacaga aattcaggga aatcaagagg gaatagctgg
    11401 tttatcagca gttgctaacc aaaaagcacg tgagcttcaa gcaatctccg acgaagggat
    11461 cagaaaatta cgtgcagcgt atcaggcagc agaaaataaa gacggtgtag acttagatac
    11521 tttgatcaat caattatcgg ctaattatac gaatcatgtc gctaagattt ccgatattta
    11581 tttgcagact agcgcctctc ttcaagcaga gtcaacaagc acacaagaca caacatcttc
    11641 tgattctgag acagcagagt cggtagaagc ttctgaaatg caagctcgaa caacacaaga
    11701 cacaacagat tcctctgaaa ctaataccaa cagcgcgact tcacaggctg cgacaaccgt
    11761 agttttggaa ggagaaggtc caaatcaaat agccgaacga actggcgtac cagtagaaac
    11821 gatcttgtcg ctgaatggga tgagcatgga gaattatttc ctgactcccg gtgagacgct
    11881 aaaattgaat tgataaaagc aataaaaaag aacgaagctt ccactaaggt gaagcttcgt
    11941 tcttttttta ttttccggtt cttttttcgc gtacaaagta atggatcgtc atgtaaagca
    12001 agaaaacgag tccaataatc aatgagattc tcgtttcagg attgataaac atgaaaatca
    12061 atacgatagc taaaaaagca agtgtcacat aattcgtata aggagatagt ggcattttaa
    12121 aaggatgatc atgcatttta tctgcgtgct tctttttgaa gcgaatctcg ctgatcaaaa
    12181 tcactaccca aggaaccata ccaggcaaaa tacttgagct gtatacgaaa acgaaaatat
    12241 tttctgactg gttgatgaac attggtagaa gataatttaa gatcagacca attaagattc
    12301 cacctgagat tgctacgaca ggataaaaag gcgcaccgtg acgagatagt ttcagaaatt
    12361 ttttagacat ctttccattt atggcaagtg tatagaccat acgacttgcg ctgaaaatcc
    12421 ctgaattgca acctgataat gccgctgtaa tcacgacaaa attaatcagt cctgcagcaa
    12481 atgtaatgcc gactttagaa aaagtttgta caaaaggtga accaatctca ctcaattgat
    12541 tccacggata gatactcaca atgataaaaa tagcaccaat ataaaaaatc aagattcgac
    12601 cgatagtgga ctggacagct tcaatgatcg tctttttagg attctctgct tcaccagcag
    12661 tcattccgat cagttcgatt ccttgatagg aagcaacaac gatcgataat gcaaagaaaa
    12721 atcctttgac gcctccagtg aagaatccac catttttcca aagatttgaa atccctactg
    12781 cctgaccatg attaccaaat ccaaataaga ttaccccaag acctgcaatg atcatcaaaa
    12841 tgatcgttat gactttgatg atcgaaaacc aaaattctaa ttcgccaaac gccttgacag
    12901 aaataagatt tgccagcata agaaacaaga cgacaatgat tccgggaata attgccggca
    12961 aatcaggcca ccaataattc atataagatc ctacagcaat cacttcactt attcctacga
    13021 caatatattg aaaaatatta ctccaagcgg tcaagtagcc agccacagga tggatgtact
    13081 cgctcgcgta atttgcaaaa gaaccagtcg aaggatcaac gtaaagcatt tcgcctaaag
    13141 cacgcataat caaatacaaa aagaatcctg caatcgcata ggctcccatc actgagggcc
    13201 ctgtccaacg tatggttgag gaggcaccca taaacagacc gacaccgatc gttccaccta
    13261 gtgcgatcat ttggatatga cgggcactaa gacttcgagt taacttttct tcttccatac
    13321 aaaaactcct ttcgttgttg agtcaacaag aaaaaataat caaacgtttc aacacatgtt
    13381 tttcattatt aggttagcct tattgaactc ttgatgtgat tctacagaaa attttcataa
    13441 aatgcgagga aaattcgaaa aaaacttttt catcattcta cttgaaaatg ctttccattt
    13501 tagttaaact atatttaaat aaaaagttag gtaaacctaa aaaggagtgt aagatttgga
    13561 acgtacggcc attgtaattc aagacctttc agccgcttat caagggaaaa ctgttttgaa
    13621 aactatcaac ttgacgataa atccacaaaa aattaccggt attatcggac caaacggtgc
    13681 aggaaaatca acatttatga aatctttgct agagttgata ccagctacta ctggtaaagt
    13741 caccttttct ggacagtcgg taaactcagt ccgtaaaaaa atagcctacg tggaacagcg
    13801 aagtgaattg gatctttcct ttccaattga tgtattaggc gttgtacttt taggaacata
    13861 tccatcttta cgaattggac aaagacctgg gaaaaaagaa aaagaacgtg caagacaagc
    13921 tttgaaaaaa gtagggatgg aagaatatgc caaaagacag atcagcgaac tatcgggtgg
    13981 acagcttcag agagttttta ttgcaagagc tctagcccaa ggagcagaat ggatcttttt
    14041 agatgaacca ttcgtaggga ttgatgcgtt aagtgaacga aagatctttg acatcttgca
    14101 ggaattgaag aattcaggaa aaacgatttt gatcgtccat catgatcttc ataaagtaga
    14161 agaatatttc gatgaggtta ttcttttaaa taaacagctg atcgcttccg gtccagtaca
    14221 agaagtcttt acatcgaaga accttcaatt ggcctatggt gagatcatca gacatttagt
    14281 aaaaggagga gagaaaaaat gatccaatcc tttattgatg gattaatgaa ttatcaattc
    14341 ctgcaatacg ctttggtcac ttccatgctt gtaggacttg cttctggagt catcggttct
    14401 tttattattt tacgagggat gtctttgatg ggagacgcta tctctcacgc agttcttcca
    14461 ggtgtagcca tttcttatat gtttggtttt agttacatct ttggagccac tgcttttggg
    14521 atgctaacag cagcagtgat tggtttcgtg actcagcgga gccgattgaa aaatgataca
    14581 gcgattggaa ttgtctttag ctcctttttt gcattaggga ttatcttgat ttcatatgcc
    14641 cagagtgcga cagatttgta tcatatttta tttgggaatg tcttagctgt tagggaaagc
    14701 gatttgttgc tgacagcact cgttagtgga atcgtcttga tatttgtctt tttcttttat
    14761 aaagaattga agatcacatc attcgatccg acaatggcaa aagcttatgg gctgaacaca
    14821 tcgctgatcc attatctttt gatgttcttt ttgacattag tggctgtagt cagtttacag
    14881 acagtaggaa caatcttggt gattgccatg ttgatcacac cagccgccac ggcttacttg
    14941 ctaacgaacc atttactgaa aatgatcatt acagctgcag gaatcggtat gctaagtgca
    15001 gttgtcggtg tgtttttcag ttatagttac aattggccat caggagctac gatcgtgtta
    15061 gcatgtaccg cattttttat ccttgctttt ttattttctc caacaaaagg aattttattt
    15121 cagaaaggaa gcagatagat gaaaacaaaa aaatcactct ttttaatctt ggcagttagt
    15181 ttcttggtat tagccggttg cggaaagcag gcttctgacc aagcggatga aggaagtaaa
    15241 gaaaagttgt cagtcgtggc taccaattcg atcttggcgg acatggcaaa agaggtcgga
    15301 acagatcaaa tagatatcca cagtatcgta ccagtcggaa cagatccgca tgaatatgaa
    15361 gtactaccag aagacatcaa aaaggcaagc gatgcagatg ttattttata caacggtttg
    15421 aatcttgaaa caggtaacag ctggttcgat aacttgatgg aaacggctaa aaaagaagaa
    15481 ggaaaagatt attttgcagt tagcaaaaat gtagaacctc tttatttaac tagcggtgaa
    15541 gaacatacaa aagcagatcc ccatgcttgg ctagacctat ctaacggaat aaaatatgtg
    15601 gaggaaatcg cacgtatatt ctctgaaaaa gatgcagaaa atgcgacact ctataaaaaa
    15661 aatgcagaag catatgtgga aaaactaaaa gaattagata cacaagcgaa ggaaagtttt
    15721 gcttctatcg aagagaacaa aaaattatta gtaacaagtg aaggggcatt caagtatttt
    15781 tcacgagcat atgatttgcc agcagcttat atatgggaga tcaatacaga aagtcaaggt
    15841 acgcctgatc aaatgaaagc gatcattgat cagataagag caaaagaagt accggtttta
    15901 ttcgtggaaa ccagtgtaga ctcaagaagc atggaacggg tagcaaaaga aacgggtttg
    15961 aaaatctatg ataaactgtt cactgattcc atagcaaaag aaggagaaca aggagattct
    16021 tactatcaga tgatgaaatg gaatattgaa acgatccatg aaggtttgtc acaaacaaaa
    16081 gaaagctgaa aagtgcaagg tctagcttga ggagcagacc ttgcattttt tataagagat
    16141 atgaaaaaaa attatgctgt ttttcgtcgt acagatacct tttttgaaga aattccgtta
    16201 tagttagagt aaggaaggaa ggtgtggcta tgaattttac acgtaaagat aaatatacga
    16261 ttaaagaaga agtctttcga ttgatcagcc agcaggaaaa taccgacaag gatcaagacc
    16321 ttcaatttct tgaagcagaa ttagctaaat tattcatagc aaatcaaaat gagacaacag
    16381 aaccagatga agagctgtac ataggcaaat ggttgtttga tgaatttcag cctttagatg
    16441 aaaataccgg tatcggttat tttgtgttcc tttctccagt cggcttcaca cctggcgtat
    16501 tagttaaaca aaacgacgaa ataaccgtca aagcatggcc tgcattattt tcagtcatca
    16561 aagcagaagg agtaatcaaa aagatccagt cggcctatta taaaagtcaa tttattgcca
    16621 acagcaaatt aatgaatatg ttttataatc gtctagtaga aattgctagg cctgggaaaa
    16681 tcccgcaaac attcacgtac acacagcaaa aaaatctcta tgaagaagta ttgtattccc
    16741 gcgatctgtc tgagttttcg ccatgggaag aggtaaggct ccatgaactg ttaagtacaa
    16801 aaccagtttc attgaatttt tttaatgcat tagataacaa aagaaaatta atttcaacaa
    16861 attattggga agtatttgct gcaagaaaaa aatggatgga agaaaagaag attgccgatc
    16921 agcagatcca gtttgtccag ctgaaaaaag aaaaactata ttatgggtta tacagccgtt
    16981 aacagcaagc gaggaagacc atgacattaa aagaagaaat catacaagaa agcaaacgat
    17041 taggaattga caaaatcggg ttcaccacgg cggaaccttt tgattcatta aaagaatcgt
    17101 tggaagaaca aaaagcagct ggccatactt ctggctttga acatccaaat atcgatgagc
    17161 gtctttatcc agaaatgaca tttgatcagc cgaaatcgat catttccatt gcacttgcgt
    17221 atcctactag gatccatgaa gaagtgccta gggatgaaaa acgcgggcag tttgctcgtg
    17281 cttcttgggg gatcgattac catgatatac tcagagaccg tttgaaccga ttgatcgaat
    17341 tcatccaaac aagagcagaa aaatggcaaa aagaagagga atggcgtcta gctccgcaag
    17401 tagatactgg agaactgatc gatgtcgctg tagcacagcg agctggttta ggatttattg
    17461 gacgaaacgg attgttgatc actgaagagt tcggttcatt tgtctatctt ggtgagatcg
    17521 ttactaatat tgtatttgag cctgataaac caggagaatt tggttgcggt gactgtatgc
    17581 gctgcgtgac tgcttgtccg acaaaagcat tgcttggtga cggaagaatg aatgcccagc
    17641 gatgtttgtc ttatcaaact cagacaaaag ggatgatgcc ggaagaatat cgtaaaaaaa
    17701 tgcgaaatgt catttatgga tgtgatatct gccagttagt ctgcccttac aatcaaggaa
    17761 aagattttca ttttcatgaa gagatggaac caaaagtaga cgaaatatat ccaaaattaa
    17821 agccaatgct ttcgatgtct aataaagact tcaaacagca atttgggcat ttagctggtt
    17881 catggcgtgg gaagaaacct ttgcagagaa acgcattgat tgctttagct aatctaggtg
    17941 atcgaacggc attgcctgag atttcagaat gtttaacaga tgtccgccct gtcatcagag
    18001 gaacagcagc ttgggctatt ggacgtctag gaacaaaaga accggaaaaa tggcttgatg
    18061 tattagatga aatattagct aaagaaacag aagaagtcgt tataaacgaa ttggaacatg
    18121 cccagaaatt attgcggaga aagttaaaat aaaaaaatag ttaacattat tcaaagtttt
    18181 tgtccagtaa ttgttgtaac aattactggt ttttttgtat tttttacatt tttttaagag
    18241 aatttgataa aaatcaaaaa tttttttgct tttttttatg gattattgca gtgaatgtta
    18301 cagaatctgc gtttttcatt caaaaataaa aaaacataga aaaacgatta ctttgcttat
    18361 acaacaaggc ttttatggtt ttgtatcaaa aaaatatttg tttaaatgtt acaaatatta
    18421 aataattcgt tattttgtca ttaaatgtca tgtttctttt acctatgaaa tatttctaaa
    18481 tgataaagtt aatagcgtcg aaagaataaa aaacggagaa aaaaaggagt ttaatatgaa
    18541 ttcatttaag aaaattgttt taggtacaac ttttgctgct ggtgctactg caatgtttgt
    18601 aggaactaca aatgctcacg ctgatgaagt ttatacagta aaatcaggtg attcattatc
    18661 taagatctca caaaaatttg caggagacaa ctcaatgatc gatgcaattg ctgaaaaaaa
    18721 ttcgatcgca aacatcaacc gcatttatgt tggtgaacaa ttaacaatcc caacttcaaa
    18781 tgattcttca gcaacaacag aaaacaaaac aacagaaaat acagcatcta caactgaaac
    18841 agcaacgcaa gaacatacat acgttgcacc tgttgaaaca gttgaagtag ctcctgctgc
    18901 cccagctgct gcaacagcgc caacttcaag ctctgcaaaa gaatggatcg cacaaaaaga
    18961 atcaggcggt tcatacacag ctactaacgg acgttacatc ggccgttatc aattagatgc
    19021 ttcatactta aacggcgact attcagctgc caatcaagaa cgtgtagcag aacaatatgt
    19081 aacatctcgt tatggttcat gggatgctgc taaaacattc tggttagcaa acggctggta
    19141 ctaagccgat aaaaggaaaa tgatgaaacc gagaaacttg tttattcaag ttctcggttt
    19201 ttttgtgtga aaaataactc gtttgcttcc cacagcgcag cagtacaaat aaaaactcga
    19261 ataatgtata ataaagaaaa cgtttttttg tttagggaat gaggaggagc catgagtaca
    19321 aaagagagtc tcgaattaaa cgaaatcaca agtggaaatc cactgttatc cagtattcga
    19381 tcgattgtag aaacgacatt ttacggtaat aatgttcatg aagtttttga taggaagaca
    19441 gcttatcaac tagcaaaagg gagtccagga acgatcatca cagatttaac aatctcacat
    19501 gctgaagaac tagatttacc agctgatgtt aggacactag tattcaatga tggaagcatt
    19561 gtgggaagaa cagcaagtgc tcggcggatc tttgaggact tagacaagga acaaagcaaa
    19621 tacgagaaaa ttttaagaga agctgtgtat caaagtcgca agcgccagtt ttaccataca
    19681 aaagtaatcg ttggcctatc tgaagaattt tctgtgcaaa gtcatttgct gattccagaa
    19741 ggatttgaga acaatttgta ttcttatttg ctgaattttc agccagcgaa ttcgaagatc
    19801 ttagagactt atcaaaagtc tgtttccttt gatgagggag atctttatat cttttgtgat
    19861 cccgaatgga cacatgaaac atttccaaat ggactcattt tgtttgatgc tctccataat
    19921 acagcggctg tgctaggatt acgttacttt ggagaattaa aaaaagcgac tttgacgctt
    19981 gcttgggcat tgggaaatcg caatggcttt attgcctgcc acggtgggat gaaacaatat
    20041 gctagtaatg accagaccta tacgatggct gtatttgggt tgtccggttc tggaaaatca
    20101 acgattacct tagcaaaaca tacggaaggt cactctgttt cgatcttgca tgacgatgct
    20161 ttcgttattt ctcgaaaaaa tggttttgct actgccttgg aaccttctta ctttgataaa
    20221 acacaagact atccgtctga tgatccggcc atttcttctt tccttacttg tcagaatgtg
    20281 ggcgtgacat gtacaagtaa aggagaaaaa gtcctagtaa caggagatat acgaaacgga
    20341 aatgggcgga cagtcaaatc acgttatgcg acacccgacc gagtcgatca tcttagcgaa
    20401 aagatcgatg ctgttttttg gatcatgaaa gatgattcgc ttccgccaat tatcaagatc
    20461 aatgacccag ttttagcagc gtcttttggt gcaacattag caacaaaacg atcagacgcg
    20521 gaaaatatca ctacaaaaag acagctagat gagctagtca ttgaaccatt tgctaatcct
    20581 ttccgtgtct atcctcttag ggaagactat caagcttttc gagagttgtt tttgaaacag
    20641 caaacagcct gctatgtcct gaatactgga tatttcgatg ataaaaaaat caaacctgaa
    20701 acgacattgt caatcgttga aagaatcgtg caacaaacgg cagaattcaa gtcgtttggt
    20761 ccatttgaag acatccacta tttacctata ccagattttc ctgtttcttt tgaggatacg
    20821 tcctataaaa atctggtgaa acaacgactt gatattcgtt tacgattcat tgaagctcag
    20881 ataggaaaag atctcttgcc agatgaagca aaagaaagtt tggagaattt gattcagaaa
    20941 ttgaactaaa ataacagctt tttcaagaag taatccatgc gcaatatcgc tcgggatcct
    21001 tacttgaaaa agctgttttt ctatgatgga tttttcgtaa tataggtgga gatgtccttt
    21061 tgataatctt ccgtaggcca atgaccagta tttaagaagt tggctaatag atagccgata
    21121 taaggacctg tggtcaagcc agaagagcct agaccgctag cagccagtaa gcttggatca
    21181 tctggcagta cgccgaaaaa tggggaaaaa tctgacgtat aagcacgggt acctacacgg
    21241 aactcgtatg gccagttttt tatctgatct ggatcagtca agaactgttc tgtactctta
    21301 gtcaatccct tataagcttc gctagtagga gccaaatccc agccttgatc gttttcatgg
    21361 gtagcaccta aaaggatctt tccatccatg aaaggaatga aatcagcttc tccgtcaagc
    21421 atagcaacag gccagtcttg actcatctta taagatgtct gaaatgctaa gagctgccct
    21481 ttttgcggac gtatatctgc agcatagcct aattcttgta ataaaggctt aagtgccggg
    21541 ccaggtgtca aaattacacg atcagctgtg tattctccaa acggtccctt gatgagccaa
    21601 gaatcgctct tcttatgcaa agtgactttt gcctggatct gctccatttg tttttgtgct
    21661 aaacgctgac caagatgttc taaatatgct tttccatcca gacgaccacc accactgata
    21721 cgcagtgaaa ctgctggttt caataacgga agtgcttgag atgtctgttc aggtgaaagc
    21781 cattcgatgt ttccaatttc tggcgcttct tcttttcgtt tctcggcaag aacagccaat
    21841 tcagctaaat ctttttcagg tcgaagcaaa agtgtccctg attgtgcgta aatcgtttga
    21901 tcaagttgga aatcttttat aagtttaggg aaaaaagcag cgccgtcttt agccagacga
    21961 taccattgtt tgtttctgcg tttcgacaac caaggggaga taatcccagc gctagcttta
    22021 gttgcctgtc ctgtcccttc atcaaataaa gcaacattga attttgatgt atctaagtaa
    22081 ctggctaggg tcataccaat gatcccgcca ccgataatgg caattttttc cataaaaaag
    22141 cctcctttta caaacaacca ttttcacaca aaaaaacggc agatgcaaca cctgccgtat
    22201 atggaatcta cgctttttta gcaaagaaca gataataggc gaatcgagca aagcttacga
    22261 gaagcaacag caacaagacg cctaatgctg cttttgatcc taatgtagta gacacttcat
    22321 agttatggga agtatgatgc tggacgtaat tgataatgac cgctactaat gaagtagcca
    22381 cagcgccaga aaactgctgc aaggtattga acattgcgtt cccgtctcct tgtagttctt
    22441 cattcagtgc attcattccg gtggtcatca tattgctgta agataatccg ataccgatca
    22501 tataaaacac atgacaagca atcaatacac ccactgaagc gtgtcctatg agtaggaata
    22561 atgctagcca accaatcaaa gaaagaatca gcccagcaaa aattggcttc ttcggaccat
    22621 attgatccag cattttcccg gaaaaaggag aaagcaaagc accaaccgct gcgccgggaa
    22681 gcatcgctaa tccagcgaca aaaggactac ttccttgaac gatctgaagg aaattcggca
    22741 agacaaaaga tacaccaagt aataaaaact gataaactaa gaagccaatc aaaaacaagc
    22801 ggaatgtttt atttgaaaga atagacagtc ggatcagtgg atgctccact tttttacttc
    22861 gatagtaaaa aaggaaccag ccaataccgc cgatgattaa agcaatccag ctggaaactg
    22921 ttcccaacgt actcaaaaat gttagacttc cactaaacaa aagacaaatt cctaaaacgc
    22981 ttatccaatc caaataacca cttttttgta ctgccatctc gggaatagca aaccagccga
    23041 taatcaaaga aagaaacaaa acgggcacaa gaaacaggaa gatatgattc catgtaaaat
    23101 gtgaagtcat aatgcctcca taagtggggc caattgctgg agcaatggcc gttgtcaaag
    23161 taccgacacc catcatcgcg cctcgtcgat tgaggggggc aaatgtcaag ataatatgga
    23221 acatcaacgg aagagcgata cctgtcgctg caccttggag caatcgacca aacaatagga
    23281 ccatgaaggt aggagagaag aaatcgatca ttatacctgc caagaaaagc agatttgaag
    23341 ctaaaaagag ttttttgaga ctaaaattgc gattcaaata agtagacagc ggaacaatga
    23401 tcgaaatcat caaaagataa atcgttgtta cccattggac ttgtgcagtc gttaaatcaa
    23461 actgtctgat aagagttggg aaagttacat tcatggccgt ctcgatcaat acgcccgcaa
    23521 aagacatcat ccctgttgca attactgcag gtactaaaag cagctgtttt tgttttgtca
    23581 tttacatcac ctctaaaaaa tttcgtaaaa aaagagagtg atttctatta taaaaataga
    23641 aaccgcctct ctctcgacac gccgaaacgt gttatctgta cgatttattt acttcactta
    23701 ttctagcaca aactgaaaaa tagtgtcaac acgattagcc aagtcactca aaaagaggta
    23761 tgataataaa taactaaatg gaatttcgcg gaggaagaaa aatggaaaaa attgaatggc
    23821 gtaaagataa gagctatagt atgaaaaaaa gcccgcaaat actagaggtt cctaaaaaat
    23881 cattctttag catcagaggg atcggcgacc cgaatcagga agactttaaa cgaagagtag
    23941 cctgtctata tgcagtcagc tatgcaatac gcatgtcgcc taaaaaaaat tggctgattc
    24001 ctgattatca gatgtatact gtctatccac tggaaggaca atggggactg caagaaaagt
    24061 atttgcatga accagtgatg aaaaaagaac atttttctta tcaattgatg atccgacagc
    24121 ctgactttgt tacagaagcg atagcacaag aagccattca gcgttctagc aaacttccgg
    24181 aagacttgaa agaacaagta gtattcaaaa agatggaaga aggtctttgt gcccaaatct
    24241 tacacgtcgg gccctatgcc gaagaaccag caacgtttga gaaattagaa gcattcatcg
    24301 caaaagaagg ctatgaaaga gcttctaaag aacacaaaga gatttacatc agcgatccaa
    24361 gaaaatcagc acctgaaaaa atgaagacga ttttgcgtgt taaaatagaa aaaggttagc
    24421 aacctgaatg aaaacaaaaa ataaaagtaa aaaataccaa aatatgaaga aatattactt
    24481 atcttttgct ataatcaaca ttgagaggtg tctaatatga aaaagaaaca gcgacaagca
    24541 ctgatccgcc agatcattac agaacaacct attgggacac aggaagagct gcttgcgcgg
    24601 ctgcatgaag caggcgccga tgtgactcaa gcaacgattt ctcgagacat tcgagaaatg
    24661 aagttagtaa aaagtcaaaa tgaaaacaaa atagtacgct atacattatt taaccagcca
    24721 tctgtttctt taaatgaaga acgtttgcgt acagcaatta gacgcgaggt tttgcgtatc
    24781 cagtctgttc agttcatggt tatcgtattg actgaacgaa atggtgccga tgtggtaacg
    24841 aattggctag atgaagtggc ttaccctgaa gttgtcggaa cgatggccgg tgtagataca
    24901 ttcattatta tttgccgctc agaagaagaa gcacagcgat ttgcagaaaa gctggaaaaa
    24961 atgagggaat aaatgaggag gacttttata tgaaagatat gaaagcgctg caaaatggat
    25021 cggatattcg aggaatcgca ttaccagttg aaggatatga agtaaacttg acgacagaca
    25081 tggtaaaaaa gatcggctgg ggactagcaa actggataaa gaaaaagaag aaatcaaccg
    25141 atccaaataa aaaatggacg attgggattg gtcatgacag taggataagc ggaccagcac
    25201 tgaaagaggc gttgatccaa agtctgaaag aacaagggat acatgtcatc gatttcgaat
    25261 tagctactac gcctgctatg tttatgtcca ctcaatttga acagttctcg tgcgatgctg
    25321 cggttatgct gactgctagc catcttccct attattataa tggaataaaa ttctttacct
    25381 cagaaggagg agcagaaaaa gaagatattg cttttatcct atcacagcag caaaaaataa
    25441 aaagcgaaca aactggaagc gtagaaaaag cagacctttt gacaccttat gcgcaagatt
    25501 tagtcacaaa aattaaaaag gggattggaa cactagaaag caaaccacta aatggttgga
    25561 aaatcattgt tgatgccgga aatggtgctg gtggattctt tgctgaaaaa gtattgaata
    25621 gcttaggtgc ggattcaaca ggttcccaat ttttggaacc agatggccga tttcctaatc
    25681 atatcccaaa tcctgataat aaagaagcga tggaaagtat caagcaggct gttttagcaa
    25741 atgaagcaga tcttgggatc attttcgata cagatgtgga tcgttctgca gttgtcagtg
    25801 cttctggaac agtgatcaat cgaaataatt tgatcgcagt attaagtaca atcgtattaa
    25861 aagagcatcc aggaacaaga atcgtgacga attcccctac ttcttctcat ctacaaagat
    25921 tcattgaaga aaaaggaggc atccaagttc gttatatttc cggctatcga aatgtaatca
    25981 ataaagcatt agaacttaac cgtcttggga aagatacaca gctagccatc gaaacaagcg
    26041 gacacgctgc attcaaggaa aactattttc tggatgatgg tgcatatgtc atcgcaaaga
    26101 tcctgatgct tttgccagaa ttaaaaagaa aaggtatcac attagatgaa caaatcaaag
    26161 agttgaaaca gcccgtagaa gtacaagaaa tccgattaaa attgttcagc gatgaaccac
    26221 gcgaacaagg aaacaagata ttagaagaaa tgaagcgact gttggaagaa gaaaaaggga
    26281 tgaacttcga cccggaaaat gaagaaggta ttcgatatga tctagaagag ccatacggaa
    26341 acggctggtt tttgatccgt ttaagcttac atgagccgtt actagtatta cagatagaaa
    26401 atgatgaatt cggaaagaac aagttggcgt tagtaaaatt agcaaaatta ttcggacatt
    26461 tttctgatct tgaatcatta acagcagaat aaaccagtct ttttgaaagg ctagttgctt
    26521 ggacggcaaa aaaagtggta tggttaaatc gagtgctggt agggagacca gcaaaaggag
    26581 gaaaaaatgg taacgaatat ggttattttt ttaacagtat tggtttatgg ggtcaacttt
    26641 ctggtatata tgtatcttaa aatcaatcat ataaaagatt atgtagaaaa gattgctatt
    26701 tattttggaa caaatatgac gctgttgttc acgagtagta tttttttgtt ttttggaaaa
    26761 atagtagaag acggcattct ggtattcgaa taagcagaat ttttgtagaa agcgccttac
    26821 ttgtcataca ctataattat agccaagtag caaatggtca atcgaaagga gcagtacaat
    26881 gaaaaaagca tacgaaacag caatgatcaa ccatggcggg cgcaatggag aagtcgaggc
    26941 accaaatggc tcgatgcata tgaaaatcac tcctccagga atccatgcag aaggaacaaa
    27001 tccagaacaa ttatttgcag caggttatgc ttcttgtttc aatggggcgg tacaacatat
    27061 gatcaaagaa gcaggaatga cagcagagtc cacagtaaaa gcacgtgtct ctctttataa
    27121 tttagatgat ggcggctatc agatcggtgt agtattagaa gtgcatatcg atggcatcag
    27181 cgaagaagaa gcccaaaaaa ttgcagaaga tgcgcatgaa ttctgccctt actccaaagc
    27241 aactagagga aatatcgatg tagaagtaac gattgtttag aagaataaag gaaatcgaac
    27301 cagactaaaa acgtgaaagg accgcttttt gtctg
//
LOCUS       DANKEF010000032        27272 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000032 DANKEF010000000
VERSION     DANKEF010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.032"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(69..437)
                     /gene="rplL"
                     /locus_tag="PWZ48_001607"
     CDS             complement(69..437)
                     /gene="rplL"
                     /locus_tag="PWZ48_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDL2261265.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(490..993)
                     /locus_tag="PWZ48_001608"
     CDS             complement(490..993)
                     /locus_tag="PWZ48_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDL2261266.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(1197..1324)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(1403..2092)
                     /gene="rplA"
                     /locus_tag="PWZ48_001609"
     CDS             complement(1403..2092)
                     /gene="rplA"
                     /locus_tag="PWZ48_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDL2261267.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(2196..2618)
                     /gene="rplK"
                     /locus_tag="PWZ48_001610"
     CDS             complement(2196..2618)
                     /gene="rplK"
                     /locus_tag="PWZ48_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDL2261268.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            2961..3629
                     /gene="sdaAB"
                     /locus_tag="PWZ48_001611"
     CDS             2961..3629
                     /gene="sdaAB"
                     /locus_tag="PWZ48_001611"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HDL2261269.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            3642..4514
                     /gene="sdaAA"
                     /locus_tag="PWZ48_001612"
     CDS             3642..4514
                     /gene="sdaAA"
                     /locus_tag="PWZ48_001612"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HDL2261270.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(4605..5624)
                     /locus_tag="PWZ48_001613"
     CDS             complement(4605..5624)
                     /locus_tag="PWZ48_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HDL2261271.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(5797..6702)
                     /locus_tag="PWZ48_001614"
     CDS             complement(5797..6702)
                     /locus_tag="PWZ48_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HDL2261272.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(6761..7306)
                     /gene="nusG"
                     /locus_tag="PWZ48_001615"
     CDS             complement(6761..7306)
                     /gene="nusG"
                     /locus_tag="PWZ48_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDL2261273.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(7407..7577)
                     /gene="secE"
                     /locus_tag="PWZ48_001616"
     CDS             complement(7407..7577)
                     /gene="secE"
                     /locus_tag="PWZ48_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDL2261274.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(7601..7753)
                     /gene="rpmG"
                     /locus_tag="PWZ48_001617"
     CDS             complement(7601..7753)
                     /gene="rpmG"
                     /locus_tag="PWZ48_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDL2261275.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            7915..8559
                     /locus_tag="PWZ48_001618"
     CDS             7915..8559
                     /locus_tag="PWZ48_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2261276.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(8615..9118)
                     /locus_tag="PWZ48_001619"
     CDS             complement(8615..9118)
                     /locus_tag="PWZ48_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2261277.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(9131..9177)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            9715..10680
                     /locus_tag="PWZ48_001620"
     CDS             9715..10680
                     /locus_tag="PWZ48_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HDL2261278.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(10779..11951)
                     /locus_tag="PWZ48_001621"
     CDS             complement(10779..11951)
                     /locus_tag="PWZ48_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261279.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(11938..13083)
                     /locus_tag="PWZ48_001622"
     CDS             complement(11938..13083)
                     /locus_tag="PWZ48_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261280.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(13097..14029)
                     /locus_tag="PWZ48_001623"
     CDS             complement(13097..14029)
                     /locus_tag="PWZ48_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2261281.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            14146..15225
                     /locus_tag="PWZ48_001624"
     CDS             14146..15225
                     /locus_tag="PWZ48_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HDL2261282.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            15222..15851
                     /locus_tag="PWZ48_001625"
     CDS             15222..15851
                     /locus_tag="PWZ48_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2261283.1"
                     /translation="MITVVIIDDDPFVTKSLQTILESTKEIRVLGIGHCAKDALVLYE
                     THRPDVLLTDIRMPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYL
                     IKQNLQAIIPSVKSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKE
                     VAAGKNNKEIADALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(15961..16188)
                     /locus_tag="PWZ48_001626"
     CDS             complement(15961..16188)
                     /locus_tag="PWZ48_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2261284.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(16291..18879)
                     /locus_tag="PWZ48_001627"
     CDS             complement(16291..18879)
                     /locus_tag="PWZ48_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HDL2261285.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(18899..19558)
                     /locus_tag="PWZ48_001628"
     CDS             complement(18899..19558)
                     /locus_tag="PWZ48_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2261286.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(19607..20110)
                     /gene="trmL"
                     /locus_tag="PWZ48_001629"
     CDS             complement(19607..20110)
                     /gene="trmL"
                     /locus_tag="PWZ48_001629"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDL2261287.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(20331..21197)
                     /locus_tag="PWZ48_001630"
     CDS             complement(20331..21197)
                     /locus_tag="PWZ48_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDL2261288.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            21306..21947
                     /locus_tag="PWZ48_001631"
     CDS             21306..21947
                     /locus_tag="PWZ48_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDL2261289.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(22009..23370)
                     /gene="mgtE"
                     /locus_tag="PWZ48_001632"
     CDS             complement(22009..23370)
                     /gene="mgtE"
                     /locus_tag="PWZ48_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HDL2261290.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(23388..24287)
                     /locus_tag="PWZ48_001633"
     CDS             complement(23388..24287)
                     /locus_tag="PWZ48_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HDL2261291.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(24289..25086)
                     /locus_tag="PWZ48_001634"
     CDS             complement(24289..25086)
                     /locus_tag="PWZ48_001634"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HDL2261292.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(25064..25768)
                     /locus_tag="PWZ48_001635"
     CDS             complement(25064..25768)
                     /locus_tag="PWZ48_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HDL2261293.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            25899..26474
                     /locus_tag="PWZ48_001636"
     CDS             25899..26474
                     /locus_tag="PWZ48_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HDL2261294.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            26592..27269
                     /locus_tag="PWZ48_001637"
     CDS             26592..27269
                     /locus_tag="PWZ48_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2261295.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         7952 a         5755 c         4911 g         8654 t
ORIGIN      
        1 agtttaacat aaaaacaaaa aagcaccgaa atccaacata ggatctcagt gcttttaaat
       61 agtaaaaatt atttaacagt tacagatgcg ccaacttctt caagtttagc tttcaattct
      121 tcagcttctt ctttagatac gccttcttta actggtgcag gagcgccatc aactacagct
      181 ttagcttctt tcaagcctaa accagttgct tcacgaactg ctttgataac tttaactttt
      241 tggtctccag ctgcagttaa ttctacagtg aattctgttt gttcttcagc agcagcagcg
      301 ccaccagcag cagctacagc tacaggagca gcagcagata cgccaaattc ttcttcaata
      361 gctttaacta ggtcgttaag ttctaggata gttgcttctt ttaactcagc aacgatgttt
      421 tcaatgttca atgccattat aatttcctcc gtttaattgt tcttttttat ttaatgcttt
      481 atgcggcaat taagctgcat ctccatcttc tttgtctgcc actgctttga cagcgtaagc
      541 cacgttgcgg actggcgctt gtagtacaga tagcaacata gatagaagtc cttcgcgatt
      601 tggcaatttt gccaatgctg tgatttcttc caatgaagag actttacctt cgatgatacc
      661 gcctttgatt tctaatgctt ttgcttcttt agcaaattcg tccatgattt tcgctggtgc
      721 gactacatct tcgttactga atgcaacagc tgtaggtcca gtgaatactt cgtccatgcc
      781 ttcaaggcca gcttttttag ctgcacgtga aagaatagaa tttttgataa ctttcatttc
      841 tacgttagca tcacgaagtt gtttacgtaa acgtgttact tcatcaactg ttaatccgcg
      901 gtagtcaacg actactactg atgcagctgc tgtaaatttt tcgcttactt catcaacgat
      961 tgctgctttt tttgcgataa ttgcttcact catttttgag tttcacctcc tgaattttga
     1021 tggaagagtt ttttgcactg aaaaggcaag tcacttttat agttactttg ctcaaccagt
     1081 attattcatc acctattcac ttcgttcata gtgattctac tcgctttgct cgaaaggaaa
     1141 ctcctttcgc tttgctcaac cagtattgtt catcatctct tcacttcgtt catagtgatt
     1201 cacaataaaa aactctatgc caccgcagac atagagggac tattgaatat attcaataaa
     1261 cgtcctcggt aggaaattaa ggcttgatgc cacctactgt cttcggtaca agttaattat
     1321 taacctcaac tagcttagca tgctaaacta gttgagtcaa gaattatttt tcagttggat
     1381 caatcaacat tctaatattt aattagaaag aagcttggtc aacatggata ccaggtccaa
     1441 atgttgttgt gatcgttaca tttttcatgt attgaccttt agctgctgat ggtttagctt
     1501 ttagcaatac atcatggatt gctgcaaagt tttcaactaa tttttcatcg tcaaatgaaa
     1561 cttttccgat tggtacatgg atatttccag ctttgtccac acggtaagtt actttaccag
     1621 ctttcacttc gtttactgct tttgttacgt ccattgtaac tgtaccagtt ttagggtttg
     1681 gcattaagcc ttttggtcct aatactcgtc ctagacgacc tacagtagcc atcatatcag
     1741 gtgttgcaac aactacatca aagtcaaacc atccgttttg gattttttga accatgtcgt
     1801 cgtcacctac gaaatctgca ccagctgctt cagcttcttt tgctttgtct cctttagcaa
     1861 aaactaaaac agtttgtgtt ttaccagttc cgtttggtaa tacaacagca ccacggattt
     1921 gttggtctgc ttttttaggg tctacgttca aacggtatgc aacttcaact gttgcatcaa
     1981 attttgcaat gtttgtttct tttgctaaag ctactgcttc ggcaacagga taaactttac
     2041 ttgaatcaac tttttttaat gcatcttgca tttttttgct ctttttagcc attttggttc
     2101 ctccttgatt gtggttataa cggttgtacc tcccacgtgt tctatatctt tcaatataga
     2161 gccaactgag aagctgctgt cttagggtgt atagattatt ctacagtgat ccccatgctt
     2221 cgtgcagttc cttcaaccat gcgcattgct gcttcaacgt tagctgcgtt taggtcttcc
     2281 atttttaatt cagcgatttc tcttacttga tcgctagaaa ctttcgcaac tttatttttg
     2341 tttggttcgc ctgaaccttt ttcgatttta gctgcttttt tcaatagaac agcagctggc
     2401 ggtgtttttg taacgaatgt gaatgaacgg tcttcgtata cagaaataac aaccggaatg
     2461 atcaaacctg cttgatcagc tgtacgagcg ttgaattctt ttgtgaatcc catgatattg
     2521 atacccgctt gacctagtgc aggacctact ggtggagctg gagttgcttt acctgcagga
     2581 atttgcaatt taacgatttt ttctactttc tttgccacga gacatacctc cttaagtccg
     2641 tgatgtggtt aattggagat gcatatttct cctcccacgt tttatctgat ctgaaaaaat
     2701 ccagacaaat aatgtgcaaa acaatgcaca ctggtacagt atagcaaatt tatttgtttt
     2761 ttcaagattt ttttctgcta tttttacatt ttgcttgaat aaacgctaaa tttattctaa
     2821 aaaaacaaca atttttcgtt ctcatttctt cttttatgtt acacttgcgc tagcatggtt
     2881 caacagatga aagcaaacaa taacacggtt ttttagaccc ttgtacaaag attcctacat
     2941 aacaaaatga ggtggagaaa atgaaagatt taaggtatag aagtgtcttt gatattattg
     3001 gacctgtgat gattggtcca agtagctctc acactgccgg agctgctaga atcgggaaaa
     3061 tcatacgtag tatatttggc gaacagcctg attctgtaga tatttatcta tatgaatcat
     3121 ttgcaaaaac ctatcggggg cacggaacag atattgcatt agttggcggc ctgttaggga
     3181 tggaacccga tgatgaacgt ttagctgatt ctttgaaatt ggcatatgaa gccggtatgg
     3241 aagtcttgtt tgttcctaag agtgaaaaag ccgaacatcc aaactctgta aagatgcttc
     3301 taaaaaaagg aacacgaaag ctttcagtca ctggcatttc gatcggcggt ggcaatatcc
     3361 agatttctga attgaatgga ttcaaaattt ctttaagcat gggtactcct accttgatcg
     3421 ttgtccacca agatgttcca ggtatgattg ctaaagtgac tagtatactt tctgagacca
     3481 atacgaatat cggcactatg acagtgacac gggaatcaaa aggtgaaaat gccattatga
     3541 taattgaagt agatgatcct caagtagaag aaacggtaaa aagactcaaa cagctgccaa
     3601 atatcgacag cgtaaattat ttcgattaag gagctaaaaa aatgttttta tcgattgaag
     3661 aattagtaga tcaagcaaaa gcttatccaa gtgtagcaga attgatgatt gcagttgaaa
     3721 tggaaatgag tggccgttcc cgtagtcaca tcatcgagat catggaaaaa aacctctcag
     3781 taatggaaca atcaatcaac gagggaaaag aaggtgttac ctccgtcaca ggaattaccg
     3841 gtggtgatgc aaaaaaatta gatgattatt taaatgaggc agacttttta agtggagaaa
     3901 caatcttaaa agctgtacgc aatgctgtag ctgttaatga agtcaatgca aaaatggggt
     3961 tgatttgtgc tacaccgact gccggaagcg cgggtgttgt tcctggtgtg ctgatggctg
     4021 tccgagatcg gcttgatttg acacatgaac agcaattgga attcttattt accgctggcg
     4081 ctttcgggtt agtcatcgca aacaacgctt ctatcagtgg tgcagaaggg ggatgtcaag
     4141 cagaagttgg ttctgcaagt gcaatggcag cagcagcttt ggtttgtgca aaaaatggga
     4201 ctcctcatca agcagcacaa gcagtagcca ttacattgaa aaatatgatg ggactgattt
     4261 gcgatcccgt tgcaggctta gttgaagtcc cgtgtgtcaa acgtaatgct ttaggctctt
     4321 ctcaagcttt tatctctgct gatatggcat tagcgggtat cgaaagtgtc attccgccgg
     4381 atgaagtcgt tgctgcaatg taccaagtcg gacgtcaaat gccgtcgatt ttcaaagaaa
     4441 cagcagaagg tggcttagct gttacgccaa ctgctaaacg acttgcggaa gagattttaa
     4501 ataagagggc gtgataaagc tccttaattt cgagtattaa gagattagtt atcttcataa
     4561 caaaaaacga agagaggact aattgccctt ctcttcgttt tttattaatc catcaatttc
     4621 gtatttttta cttcatcgat cgtttttgtt cctgcaagtt gcatcgtgat cgacaattct
     4681 ttgttcaggt gttcaaaaac agatgttact ccttctgccc cacctagatt caacccataa
     4741 atcactggtc ggccaatcgc aactaagtca gcaccacttg ccagagcttt gaagacatgc
     4801 tctcctcgac ggacaccaga gtcgaaaatc actggtacac gtttgttgac tacttcggca
     4861 atttttggca atacttcaaa ggacgcagga cctccatcta attgacgtcc accatgattg
     4921 gatacccaga taccatctgc ccctgcagag atagcgatta ctgcatcttc tggcgattgt
     4981 attcctttta cgataactgg aagatgggtg atttctttga ttgttttgat gtcatctggt
     5041 gtcaagcctt gttttgcagc tgcataaatt tctgcgattc cttttccttc gccattacca
     5101 cttgcacttt gttcgctata cgcagctaag ttaggcattg gtaatggaaa ttggaattga
     5161 ttgatgacgt cttcttcacg gtaaccaccg agcgttgaat ctgccgttaa aatgatagct
     5221 ttagcccctg cttctactgc tttgttcaaa atgaactcat tgaatctgtc gtctttactc
     5281 atatataatt ggaaaaactg cggtgcgcct ggcgctgcat cggctgcgtc tttgattgtc
     5341 gtatttgcat aagtgctgat ggaaaagatt gatccagcag cagctactcc tttagctgta
     5401 tcagcttccc ctttttcatg tgccaatcct tgggccgctg atggcgcttg gataatcggt
     5461 gtatctaatt caattccgaa aacagatgta tgcaaatcgg ctgagtcgat tccgcgtaat
     5521 atccgtggca tgattttctt tgtattaaaa gaagtcgtat tttctttcat cgtccactca
     5581 tcttcagaac caccgcgaat atagccaaaa gctcctgctt ccatgcgctc ttttacacga
     5641 ccttctagtt aggcaatgtt tacaatatcg attggatgtt cttcattact tgtatgataa
     5701 gtcatagtag atccttcttt catcttcttg attacatata aatcatacta aacttacaaa
     5761 aagtaagcaa acatttactc tcagatactt ttatcgctat tctttccaaa taaatcgact
     5821 tacaagctga tctacttctt ggttttcatg aagcatacta tgagaggcat tttcacctgt
     5881 cactactttt tcctctattt catttccgcg ttgcttcaac aacgcataga ccgctaatgc
     5941 actgtttaac ggtactgttc catcactcaa gtctgtttca tctagttgac cagcaatcaa
     6001 gaaaaatcga gtggttatag gaatattacc gatcatttgc tggtaatctt gatagcggtt
     6061 agaaacgaca gcaggcccct ttgctaactc atctgtcaga gactgcgcac tatcatcgac
     6121 aaaatcatta aaaggcgcgc caattgctgt taatgtctga acttgtggca atgacgtatc
     6181 ctgtccatac gttcctaaat agcgtagtgc actaactcct cccatggaat gccccactat
     6241 gttgacttta tctatgccgt agttttcttt cagatacaaa agacaagttt tgatccattc
     6301 tgcttgattc cactcgttat ttttattatc ttcaaacaat acttggacca tcggattgtc
     6361 tgcttttcca gacaaatctc ctgttgcttg tatttgtcca tctgttccta ctgttaaaac
     6421 caattctttt actgcttgct gatttttttc caaacgccgc aacatccctt taaaggaatt
     6481 ttctgtcccg ctgtagccgt ggaaaaaaag tgttggaata gtggtttttt ctatcacttt
     6541 actggattct gccgtttttt ctttatcaga aacttcagta gtatttatct tcgtcgtctg
     6601 gcaaccactt aacgcaaaca aactgataag gactaaagct gctgcagttg cacgtttgca
     6661 taaatttttg attggttctg tcatacacct cttcctttcc atatagaaaa aagaaagact
     6721 tgaaaggcca atcatatgat cgacatttca agtctcttta ttaatcgatt ttatctactt
     6781 gatcgaaatc aagttctgta ctggtttcac gtccaaacat atcgatgttg actttcaatt
     6841 tttctttttc tccgtcaact tctgttactt gtccttctaa tccagcaaat gcgccttcga
     6901 tgattttcac gacttcgcca acttctactt ctagatcatt ttgtcgcagg ctcataccga
     6961 tagaacgcaa gatatgattg acttcttctg gaagtaatgg tgctggtttg cttccggctc
     7021 cgtgtgaacc aacgaatcct gtcacaccag gtgtgttgcg gacaacatac catgaagcgt
     7081 ctgtcatgat catttcaaca agtacgtatc ctgggaatgt tttatgaacg atttcttttt
     7141 ctttcccatt tttgacttca gtttcttttt cttcaggaac aacgacacgg aaaataaagt
     7201 cggccatttt catactttgc gcacgagatt cgatatttgc ttttactttg ttttcatatc
     7261 ctgaatatgt atgcaagaca taccagtttt tttcaaaaga ttccattgaa ggctcctcat
     7321 ttcattgtaa aataaaaaaa cctcagtaca cccgaagttt ttctttcgct tcattatagc
     7381 actaaaagta cgttgtggct agcgagttat tttaaaatcc agccaaataa gctttggatg
     7441 gctgtatcca ttacgtaaaa catcaatccg aaaataatag aagtttcgat gactactaac
     7501 gtatctttac ggagttgttt tctgcttggc caagttactt gtttgatttc atcttttacg
     7561 ctttttagaa atttcatgag acaacctccc ttgctactgt ttatttcgtt tctttatgta
     7621 atgtatattg attacaatat ttacaaaact tattgatttc caaacgttcg ccgctttttc
     7681 cttcactgac tgattttgtg tagttgcgag acccacaaac ggaacaagcg agtgctgctt
     7741 ttttctttgc catttacttc tgcccccttg ctgagaatca gacttatact tcataacagt
     7801 atcacgtaac tttttacttg tcaatttgtg aaataaacta tctattgttt tggataaaat
     7861 tatgatatga ttaatttgaa attaagctaa aatgagacaa ttggaggtat agcgatgcta
     7921 ttaaaaacac ttgtatataa gaaaaaagat ctgacaaccg ttacagagac tagtacatta
     7981 gaagaagcat taaaaatttt agaaacttcc ggataccgtt gtgtgccgat cttagattct
     8041 tcaggtaata ttttccgtgg aaacatttac aaaatgcata tctatcgtca taaagcaaat
     8101 ggcggtgata tgacgcttcc tgtcactcat cttttgaaaa acgctacaaa gttcatttac
     8161 ctggatactt ctttcttcaa agtattcttt acgatcaaag agcttcctta tattgctgtc
     8221 cttgacagtg aaaatcattt ttatggtatt ttgacacata gtacgttgct taatatgctc
     8281 tctcaatctt ggagcgtaga acaaggaagc tatgtactga caattgcttc cacaggaaag
     8341 caaggagatt tggctgctat tgcaaaaatc atttcaaaac acagcagcat tgccagctgt
     8401 attactcttg atatcgaaaa agacgaatac attcgacgta cattgatcac gctaccggct
     8461 aacactgaag cgaaagtatg tgaaacgatc gtcaaccact tagagaaaaa gaattataaa
     8521 gtggtagaaa ttgaaaattt gcaaaaccaa gaagcataat caaaaagcga aaaaagaaaa
     8581 cagctaatct gctttctttt ttcgcttttt ataattagac acgcttttca aaatcaagcg
     8641 cgcgatctaa ataatacatc aatggtgctg aaacagccat gacgataaac tcacttaatg
     8701 caagcgtgcc ataggttgtc caaaatgctt ggccacctgt tggaactaac atataagcaa
     8761 tcagtcccat gctgatggta aagaagatcg tgttcaatac taaacgcatc tttacatttt
     8821 tcactttgtt ttgcaataaa gctgtcaggc ctaaagaaag caatgtttgg ccgccaccaa
     8881 aaaccacatc catgatacca aaaccaaaga aggcattata gacgatgacc ccgcctaaaa
     8941 ctccccaaag caatttacgg ttaaagacaa ctaaatgatt caaactttct gaaatacgaa
     9001 actggatcgg ccctgaggat acaggcgcaa ctaaaatagt taacgctaga taaagagcca
     9061 tgatgatgcc atttacaacg attacggata accgttgtct gcttttcgtt ttttgcattt
     9121 tctctcctct tttctcctag tttttttaac gcgggatggt tgatgaaccg cggttatgtg
     9181 aaacaaacaa taattataac agacttttca tttatttcaa tccgtcttat ctgcttttaa
     9241 aaaagcaaaa gcttaaacaa tgtaaacgtt tgctaaaaat cgtgggatat ggtataaagt
     9301 aagtgtaagg ggtcataaga attcccagta aagcagacga agtaggatct gcaagaacag
     9361 aattgttgtg aaacttaaat aaataaaaaa ggatgtggga atttgagcaa cctcttacta
     9421 gagaacggtc tagcaagcag ctgaagatct aagtggacat gccgaacctt ggaacttctc
     9481 cttacgtaag taatggatca tgcgcttgct aggctttttt gtgtttttcg gtcttcggac
     9541 ctttttcatt ttcctagact tctgctataa taaccaagaa ttaattttgt aacagatttt
     9601 tttgaaaaaa caagtttctt tcattcaaaa aatagaaaaa gcttgctaat tattggttaa
     9661 ccagcaaaca ggagtattaa aatatcttgt tttattgaat agcaggagga aatcatgaaa
     9721 ttccattatc atatattgat caatccatcg gcaggcagcg gaaatggcta taaagtagca
     9781 gaaagaattt taccggtatt aaaaaataag catattgact atacacttca ttatagtgaa
     9841 tacaaaggac acgatgctga gattgctgaa actttggcaa aagaaacatt gattccttgg
     9901 atagaagaag aacagaaaac aatcgatact ttcccccttt tgatcatcgt aggaggtgac
     9961 ggcaccttac atcaggtact tgatactttc tatcagatgg aagtagaatt cccagttgct
    10021 tatattccag caggttctgg aaatgatttt gctcgagggg caggccttcc aaaaaatccc
    10081 aaaaaaggac ttcagctgat tttagcagct caatcaccag aaaaagtcca cataatggct
    10141 tatgaagaaa aaataagtga aaaaaaagga atagctgtta ataatttcgg tataggatta
    10201 gatgcagcta tcgtccatgc gacaaatcat tcttccacaa aaaagcgatt gaacaaatac
    10261 aacttaggat cattttcgta tttattctct attctccgtg ctttattcac tcaaaaagga
    10321 tttccaattc tggtagatgc aggaggaaaa caattaagtt tttcaaacgc atttttatgt
    10381 acagcaacga agcatccttt ttttggcgga ggaatcgcga ttgttccaac tgctgatgcg
    10441 tctaaaccag tcattgattt tgtcgtggtc gaacgaatca atttctttaa aatcttgtgg
    10501 ctcattcttt tacttataca gaaaaaacaa ttgaaatcga aatatttcca tcactacaca
    10561 acaaacagat tacgtatcgt ttcaacaaca ccgcaacatg gtcaagaaga tggagaagaa
    10621 atggaaaaac gaccttttga tatcacaata accacaacta aacagttgtt ttggtgttaa
    10681 taaaagaggt tttgtcagaa gctacatctg gaacaccgtt tattcggaaa aaaggactgt
    10741 gacacgactt ttgtcacagt ccttttttta ctatgcagtt aattctttgc ccaattgggt
    10801 tgccatcgat agtttctttc tttacccaaa ataaattgga tcattgaaaa caccgcagca
    10861 aaagcaacta agacacaacc attgaaccaa aaagattctg caaatgtttg attgtaactg
    10921 agtgtttcac tgatcaagtt attattttga acaaaccaat atgttggtgt gaatgccgct
    10981 attttgctaa ccatttctgg caagtattct gctggtacaa acaccccacc gataaagcaa
    11041 ctgcctgtga taaaaatatc tttgatccca ccgctggttt ctgagttttt taccaatgaa
    11101 gtgatacaag ccgaaaagct aatgatcggc aataaaaaca agaaagtatt taaaatatga
    11161 taccataccg ttatatccca cgaggtgtgg atcactaaca gaaccattag aagtaagaag
    11221 ccactgaaca acagcagcga gtaactaatc aaactaaagg ttactttgcg attcaagttt
    11281 cgtttggtaa tgggcgcact ctggtttcgt ttactgatct ctttgcgatt aaacgctaga
    11341 ttgatgacgc tgaaaccgct aaaaatggat agaaacaacc cataagacaa gaaattaaag
    11401 gttgtgcccg tcaacagctt tttcatccgc tgactatatc ccgcacttag ctcaacttct
    11461 ccagtaaccg agagtgccat ttgtgtctgt tccatagctg catcgattgt tttatcttct
    11521 tcttgaaaca gaatcagtgt ttgcaaaaag gtgttcactt gctgtgtaac aactgtttta
    11581 gaaaaggcat ctgggcgact ctgtatcttg atcgtcgcca tctttcctac ttcgacttgt
    11641 tcggtaaagt ctgcaggcag atgtaagata atgtttacac gattaaaata aagagcatca
    11701 tcgatttctt tttgtgaggt tccatccaat gtaacgactg tttccttgtc attcaaatat
    11761 ttacgaaggc catcgactac aggcgactct tcttcactaa tcaaggcgat tttcgcatct
    11821 tgcagagagg tattttcaac atcaatattt tgcgcaaaaa agaatgtggc aacaacagtg
    11881 ataattacac ctatcagtaa attcgattta tttgctttaa gcaatgtcca aatcgtttta
    11941 taaagcgtca taacgcaccc ctctttccat ccaagcattt cctaacgcaa aaaggaaaat
    12001 aaatccgatc aaccatagaa gattggtata aaaactagcc aaatcagtgt agaaataaag
    12061 tttatacaaa ctttcgctga tcaagttgac gagatttaat tgaccaacaa tcggaatatg
    12121 aagatcaatc caatacttca cttgttccga ccccatcata ccagcaaaaa agctcatcgc
    12181 aatcgtcact gaaatcaaga tgctatcctt ttgcaccaga ttcatttttg gtatcaaatt
    12241 accaaccaat gtacctaaca aaatcgcaca caacgacccc agagcggtaa caatcatcaa
    12301 ccattgccaa cgttcaccaa agtctacttg atagataaaa cgaaagacag ctaacataat
    12361 ataaatctct gtcaaaacaa tcgtaaagct tgctgccaaa ttagcgattg aaactaacga
    12421 ttgctttcta ggaatcaagg acaagcgaat accatttgac gattgatttg cttgctgatc
    12481 ctgtacattg cggacccccc acatcgtccc gtagagaatc gccattccaa tcagagtgaa
    12541 gaaataaaaa cttttgaaac tcatattacg acctgcatga acttcttgaa aagatacttc
    12601 ttgttcaaaa acagtcatga tgggcgcgag atcaacgttc tccacttctg ccactttact
    12661 cgaaattata ccaatacgct gtaaaaattg atccatgacg tttttcatca acgtctgact
    12721 cattcctgct tgacccacat ggagcgaaac agatgaacca tcaatgagat aaaaaccagc
    12781 caaatcatct gcatttagct tttcattcgc ttctacttcc gatagttcct gtacttcaaa
    12841 taatggttta tcattgactt cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc
    12901 tgtttgatcc gctttgatta caattccagt attaatcgtt tcaagattgc caccttgatc
    12961 aatctcgcca aaggccgcca taaacatcaa tcctaaaaga attggaaagg ccaatgtcca
    13021 aaacaacatg gatttatttc gcagcagtat ttttgtgcga tatttataaa aatgccaaaa
    13081 cattatttcc cctcctctag tctctcaaat ccttgccagt aatctctaaa aagacatcat
    13141 tcaaggacgc ttctttggtt gctatttttc cgtaactgat cttttcttct tgtaagattg
    13201 taagaatcgg caacaatact gtatttagtt cagtagttgt tacttggagc tgaccttttt
    13261 tataagcgat tccatcagaa gctattgttc gctgaatcgc tgacaacaca ttttctggga
    13321 gtaatgggat gtcgatcgtc aaacgatccg ccgtccgtac catatctttt agagattctt
    13381 tggttccttt agcaatcacc tgtccttgat ccatgatcac gatctggtcg caaagaattt
    13441 ctacttcttc catgtaatgt gttgtataga caatggttgc tccttgtcga ttcagctcct
    13501 tgatactttc taaaatttta ttccgacttt gggggtctac agctactgtc ggctcatcta
    13561 aaaaaattaa ccgcggcttg tgggcaatcc cacatgcgat gttcaaccga cgtttcaagc
    13621 caccactaag ttctcctgga cgaaacgaaa catatttttc taatcccact aattgaatca
    13681 cctcttcaat caaggctttc cttttctctt tttcggaaac atataaaccg caaaaataat
    13741 cgatattttc ttgcacactt agttcgtcaa agaccgcaac gtcttgagga acgaccccaa
    13801 tcattgcctt gacagcataa ttttcaggag tcattgttgt cccgaacaat ttaatggttc
    13861 ctttgtcata atttaacaaa gaaagcatac agttgatggc agtcgatttc ccactgccgt
    13921 ttggacctaa cagacctaag acttctcctt cttgtacttg cagatcaaaa tgattcaatg
    13981 ctacaaaatc tccatagcgt ttgactaatt tatcaatttc gataatcatc atgaaaaact
    14041 ccttccaaat ttcttgttga tctgactata ataaagtgca agaaaagaaa ctagtgacga
    14101 ttggctaaac cacacatgac atttgtcaca atgaaggagt ctgctatgca taaatctcgg
    14161 ctactcaacg aaactagctt tctactggtt atctcattgc ttttgctggt aagtcaagga
    14221 ttcgttgcta tgcaagttat ctatttattg atcgccgttt tattcactgg tgtcagttat
    14281 ttactgccta caaagccaaa ttggattttc caagttattc tatttctttt atgtgccttt
    14341 ttacctgatc ttgtcctgtt tctgccagcg acgatgcgag tgatcgcccc tgaagaaagc
    14401 caccttgatt acttgttttt gttaggtttt ttgttgattt cgacggttgg taatcatccc
    14461 cccatcaagg tcgcctttgt cgtaattttg ctgttttttt ccttttactt acgaaaaaaa
    14521 gatcaacgaa cgaccgatct tgagaaaaag tacattgtct ccaaagatac tcattgggaa
    14581 aaacaacaag agttatcgga acaaaataaa gcattggccc tgtcacaaga aacacaatta
    14641 gccttgcaaa ttgtggaaga acggaaccga attgcccgag acatccatga caatgtcggt
    14701 catctgctgt caagtgcgat tttacaaatt ggtgcgcttg aaatgatcaa tcaagaacca
    14761 aaactaaaag aaccattaga aaatttaagt gccaccgttc acactgggat ggatcggatt
    14821 cgtcaaagtg tccatgacct ccatgagaca tcgatctctt ttcaacactc cttagagttt
    14881 ctgatcgctg attttccttc ttctgttacg attgaaggaa acttgtttga aggattgaat
    14941 gaaacgcagc aaaactgctt cttaatggtc atcaaagaag ccttaaccaa cagtatcaaa
    15001 catagtcatg ccaaaaccgt tgttttacat tttcgaacgt tgccggcctt ttatcgcttg
    15061 caaatcacga atgatggcac cagcccacaa aaaaattctg ctcccggtat tggtttgaaa
    15121 acgatgcgtc aacgtgttca agaacaaaag ggacaagtcc atattttcca agaaaaccag
    15181 caatttcagt taacgatcat tttaccaagg gagggacacc aatgataaca gtagtcatta
    15241 ttgatgatga tccatttgtg acgaaatcgt tacaaaccat tttagaaagc acgaaagaga
    15301 tccgtgtgtt aggcattggt cattgtgcca aggatgcgct agtcttatac gaaacccatc
    15361 gtcccgatgt tttgctgacg gacatccgca tgcctaaaca aacgggcatc gatgctgcca
    15421 aagaaatttt ggctaaattt cccaaagcca tccttttgct tttgacgaca ttcaaagatg
    15481 aagaatacat ccgcgaagca ttttccatcg gtgtcaaagg gtatttaatc aaacagaact
    15541 tgcaagcgat tattccttct gtcaaatccg cttataatgg acaggccgta tttggtaatg
    15601 aaatcatcga gactttcact caaatgatga agaataaacc gacgattgat agtcatgctt
    15661 atgcttcttt tagtgaacgt gagcttgcaa tcatcaaaga agtagctgcc gggaaaaaca
    15721 ataaagaaat tgcagatgcc ttgtacttga gtgacggcac tgttcgcaat tacatcagcc
    15781 aattactaga aaagctagat ttacgggatc gcacgcaatt ggctatctat ttttatcagc
    15841 atttgcaata agtcttatct aacacaaaaa aacctgaatg atgctccttc gcatcattca
    15901 ggtttttttc acatcttcta ttcaattgtc ccgcaaacca tttcctcacg attgaaagaa
    15961 tcaaggattc gcagggctgc caggattcgt tggattgttc ggcgtgttag gcggaaaaat
    16021 aggcgccttg tctaacgttt tttctagttt tgattcttca cgatcgtctc gttctacatc
    16081 aggactttca ttcaatttga attctttttc catctcatct aactttttaa agtcattgcc
    16141 agattctttt gctaatttcg ctaatttatc ttcggtttct ttagccataa agtaacgccc
    16201 cccttttgat ttcagcttag catatcacac tgaaaaaaca aaagaaaacg ctaaccaacg
    16261 atcattttta gctatttctc atctttaaaa tcaagcattt tttgccggta taaaatcgta
    16321 gggagaactg ttttccatac caatcatcgg atctactaac tggttatcga tcacttctgc
    16381 tgtaagaacg aaatctttag aaacagcagg ttctacctct tctttctttt cttctccaac
    16441 aaatgaagaa aacagatctg gttccactgt ttccattact tggtttttcg tttcattcgt
    16501 cccactgtca tcttctacag gtagcttttc ttttacaacc cgtggctctt ttgtatagtt
    16561 cccatcttca aatggatcat ttccgaccaa agaatcttca taagctgcta gtttggtccg
    16621 gagagtgata ttcattttat cattatccat gatccttgat ttcaacgtag tcatccggct
    16681 tgctgattca ttcgctacac aagtgacata ggcttgctct tcccctaaca aaatcaacag
    16741 ttccttactg cgagtcactg ccgtgtagag caaattacgc tgaagcatcc gctgatattg
    16801 atggaccatg ggcaaaataa ccatcttgaa ttcactgcct tgtgatttat ggattgagca
    16861 acaataagaa agagtgattt tattccattc gtttctttta taagtgacct catttgcatc
    16921 aaactggata actaattcat ccactttatc ttcagaatct tttgccaaga tgatacccgt
    16981 gatttctccc atatctccat tgaatacatt caattcggga gtattgacta attggaggac
    17041 tttgtctcct atccgataaa cggtgtcgtt ccaatgtact tccttttttc caggtttccc
    17101 tggattgaat atctcctgca tcattttatt taacgcatcg atccctgcag gtccccgata
    17161 cattggtgct aatacttgga tatcttgtgc acggaatccc ttatttttcg ctttttccac
    17221 aattttagag atcaagggtt caatttgata agcatcactt cggaaatagg aacgatcttt
    17281 ttgattcttt gtgaaatctt ttggcagacg tccttctttg atctcatggg caagaggaat
    17341 aatactagaa ccatcacctt gccgataaat ctgattgagt tccattttgg gaatttgttc
    17401 gatttccaaa agatcatgga gtacttgacc aggaccgaca gagggaagct gatctttgtc
    17461 tcctactaag ataacttgca tattcgtcgg aatcgctttg aataatgtgt tagccagcca
    17521 tgtatcgacc atagacatct catcaacaat caaaagtccg ccttctaatt ctttcgtact
    17581 gactgaaggt gttttttccc gtccattcag ccccagaaga cgatggatcg tactggcagg
    17641 cagacctgtc gtttcgttca ttcgtttagc tgctcggcca gttggtgctg caagtagaat
    17701 aggaaaaatt tcttgggtat attttgaagg atccaattcc agtccgttca actctgcaaa
    17761 aagattgacg attccgttga tcacagtagt tttccccgta cctggtcctc ctgttaaaat
    17821 aaatagaggg gaacggattg cttcagcgat agcttgttct tgcgaaggtc catactgtat
    17881 gccgaatatt ttttcgatac gccgaatatt cttctgcact tctttttcag gataatggat
    17941 ttctttttta cgactgagaa ggcgctggat cgaattaccg atcccccatt cagaaaagta
    18001 caaactgttt tcgtaaagat tagtctcttc ttgttgtatc tttccttctt caacaagttg
    18061 gataattaca gtagcaacct gatcaggctg gatttcaatt gggcgacttg tttctaaaat
    18121 acggatcgtt tgctgaagca atgtctccgc cggtacgtat gtatctcctg tttgaactgc
    18181 atgctgaaaa atctgatgca agatagctgc tcgaattctt ctcggtgaat ctgctgcaat
    18241 accgatttgt tcggctagat tatctgcacg tttgaacccg attccttcta tgtcttcgac
    18301 tagctggtac ggattttctt cgatgatatc caaagactcg ttcttatagg cctggtagat
    18361 actgaaagct aactgactac cgaacccgta acgattcagt cccatgatca cttgatccat
    18421 cccgtggttt agccgaatcg tctcagcaag catttctctt tttggcttcg tcaaaccgga
    18481 gatttgtttt aataccgaag gatcagcaat gattttttcg atagcatctt ctcccagcaa
    18541 ttctacaatt ttttctgctg ttttcttccc gattcctggg aatttttcgc tggaaagata
    18601 cgctacgatc ccatttgcag ttgttggctt ttcttgctca tatctttcga gtttaagctg
    18661 ccttccgtat ttaggatgat ccactaattc tccataaaaa cgataagtct cattttcttg
    18721 aatatcgccg aaacttccgg tgactacaat ctctttttca cggtaatcag ctgtggtttc
    18781 actgatgtgt accaaaagca ctttgtaaaa attactagga ttttgaaaaa aaatcgcggc
    18841 aactgtacca acgaaataag gcatttcttc tggctgcatc ggaagccgcc tccttcttct
    18901 atagtaaagg ttctggtgct gtttcagttc ctaaaaaatc tgtttggttc catactttca
    18961 gttcatatgg caataatctg caaggctctt cagtctctaa aatcgtcaaa ctactattga
    19021 acaatccacc catttcacgc aactcactta gctcttttcc tgtcatccat tgaatggctg
    19081 cagttaaaga agctccatgt cctacgaata aaagtgggcc atctttatgt cgggcaaccg
    19141 catctgctac aacagtttcg atacgtgtga ttgccgattc aatcgtttct ccgtgaaaag
    19201 gagaaggatc atacagatct aaatgattac gcaaattcgg cagattatcc ggatatttct
    19261 ggtacatttc atcgatcgac tgaccttcta aagtacccag ccctagttct cttagcttat
    19321 ctgtataaaa aatctctacc ggacgagcaa gctctttgtt gataccttct gctgtatcac
    19381 gtgctcgctt tgctgtactg gaataaattg ctttaaaagg aacatatttt acctttttcc
    19441 caaatgcttt gatttcttcg taactttgtg gcaaaagcgg agaatcgcca ttgctccctt
    19501 gaaaacgtct ttcctgattc cattgtgtct tcccatgtcg ggtaaaatat agttccatac
    19561 gcagcaaact cctgtctttc ttttatttta ctctataata aactgtctaa tccagcttgt
    19621 cattttcata gtgatgcgtc aattctaaat tagggaatcc ttgctggcga agtgcttcat
    19681 acactatcat tgcagctgta ttcgataaat tcaatgagcg gacatgctca tcattcatag
    19741 ggatgcgtaa acacttttct tcgttttcac gcataaatgt ttctggcaaa ccggttgttt
    19801 cttttccaaa taaaaagaag tgctcttctc catcagcaaa attttcgtca ctatatactt
    19861 tatgggcaaa ttttgagatc aaatgtagtt tacgattacc taaaaatgtt aaaaaagctt
    19921 ctaaattact gtggtaagta atatttacat cattccaata atccaaccct gcccgtttta
    19981 gatgtttgtc atcagttgaa aaacctagtg gctcgattaa atgaagcgga gtattcgtgg
    20041 ctgcacacgt acgggcaata tttccagtgt ttgcagggat taatggttca aataaaacga
    20101 tatgattcat caggatcatt cctctcatat ttatgttcgt ttctattttc tgacaatttg
    20161 atttttgcat aaaaaaaaac gtatcaactc ggtgtcagtc actgcgagtc gttacgttag
    20221 tgaagctctt ctcactaact tttatcagaa caggaacctg ataaaaacca atgaaaaaac
    20281 aacttgttgc taatataaca ccctcaactt ttttttgcaa cctctactca ttaaaaactg
    20341 ctagaaaaac gttttctctt agttcctttt aataattcta tgtcaattgt aatcttcttc
    20401 aaaggattct tatataagtc tgctttgatt cgctcttcta cctgccattc taaaggtgac
    20461 atggccaaaa gatctttttg attttcctct ggtatctcaa attcataggt tacacgctga
    20521 cgttcagtta atttcattgc ttcagcaaac ttggctacta cacgttcatt ggaataactc
    20581 tgtttttcag gttgattcgg ataaaaagcc tgacgtagtt cctttaaata attttcttgt
    20641 ggcaccacct tcaacacttc tccatcttct gagagtacac gttggaattc ttggtaatga
    20701 gaaggcgaaa agatatttaa aaccgcgttg aatgtttgat cagcaaacgg caaattggtc
    20761 aaatcagcta cacaccaaaa tgcttcggca gattgttctg tagctgcgta gactccttct
    20821 tttgaaatat caaatccaac agcattttga atctttttca attttgaaag ttcattcaag
    20881 aagctacctt ctccgcagcc aacatctaat agcgagactt cttcaggtaa atgctgcgca
    20941 atagtctcaa taacgggctg atacatgcca gattggatca ttctacgcct tggaacgaac
    21001 attccttgat cgtattctgt tttaaccgca tgggataaaa aataaagtgt tccttttttg
    21061 gaaaggtcaa aacggtgatt atttttgcat atcaagccat tatcagactt ttttacttct
    21121 tcttggcaaa ccggacagcg gaacagctga tgattttctg ccaaaaactg gcgtcctcgg
    21181 tcaatttttt ttaacatagt tccccctcat ttctgtttta tcttaccgtt agatgctcac
    21241 tttggcaact gctaaaaata tgatacaatg cctagaagaa agtgaaagat aaaggagaag
    21301 attagatgat caaagaattt tgcgctgaaa actacacatc tattcctcta gcaattgcta
    21361 gaggagccga tcggatcgaa ttgtgtgaca acttagcggt cggtggcaca accccgagta
    21421 ctggtgtgat cgaagaaacg ttaagttatg ctggggaaaa agatatcccc gtaatgacga
    21481 ttattcgtcc tagagggggc gacttcgtct ataacgatat cgaattgaaa atcatgcata
    21541 ctgatttgat tgaagcaaag aaattaggga cggatggcgt agtatttggt tgtcttactc
    21601 cttcaggctg gctagacgaa gaggccttag aactattgat cgaaacagca gaagggttgc
    21661 agatcacctt ccatatggct tttgacagta ttcctgaaga tcggcaattc gaagcaatcg
    21721 attggctggt agaacatggt gttgaccgta tcttgactca tggcggtcct gctgacaaaa
    21781 ctattgtaga aaatttccca cggcttaaag aattgattgc ctatacagat ggccgcttaa
    21841 tcattttgcc tggtggtggt gtcactgcag agaatgcagc acatgtagct aaagaattga
    21901 acgtaagcga agtacacgga accaaaatcg tggatcttca gttataatct tataaacaaa
    21961 aaaacatttg caacaggcat aatgcttatt gcaaatgttt ttgttttttt acataaaata
    22021 gcccataaat aaagaagcaa tattgaaata aatcagtaca ctcgtcaaat cgctcaaagt
    22081 cgtgataaag ggaccgcttg ctactgctgg atcaaatcct agacgatcca ttagcatcgg
    22141 gattaaacta cctgcaagat tcgccactgt gattgcaaat agcatagcca tccctatgac
    22201 aaaccccaag ataaaattat gtttccaaaa acctacgatt agaaaaatcg tgatccccgt
    22261 aacagctcct gtgacaacgc cggtcaagac ttcagccaaa acaagtcgaa caaatccatg
    22321 atctttctca tcagaaatcg caagtcgacg aacagccaca gctagcgact gggttccagc
    22381 attaccggca gttcctgtaa tcagcgaaat gaagactgct aaaatactcg cttcacttac
    22441 caagtcttca tagtgactga ttaaagaagc ggtcgacata cctaaaaaca acagagtgat
    22501 cagccaaggc aaacgtcgag acgctgcctt tactggattt tcattaattt cttccacatt
    22561 gaccccggca agtcctgaat aatcgctagc agcttcatca tcaataacgt cgatgatatc
    22621 atcgactgtg acgattccta acaaatgatc atcataatcg gtaacaggaa gagctaagaa
    22681 gtcataatct ctaaatgttt gtgccacatc ttcttggtcg tcgcctacat gaactgaaag
    22741 cactcgttcg ctcatcagat cagaaatcat tgcgtcgtca tcattcacga ttaggtcgcg
    22801 cagagaaata acccctacta gctgaccttc ttgattaacg acatagatgt aatagattgt
    22861 ttcagctaca tctgcttcgt tcttcagcac gtacatcgca gagcgaacag tttgattggc
    22921 tacgatcgaa acaaattcag ttgtcatgat cgcgccagcc gtttcatcct cgtaatggag
    22981 aagttccttg atttcactgg catcgtctgc cgacatcaaa cttaaatatt tagcaatttg
    23041 ctttttatcc agcgtattaa gcaagtcaac agcattatct gtatacatct cagaaagcat
    23101 atcagctgca tagctaggac gcatttcatc taggtattct ttcatatgtt cgttgtcttc
    23161 ttcaatcaca tcgaacatat ccgctaattc tttaggcgac agataggaat agatcagctg
    23221 cctgttctct tctgtaagag attggtagaa ttgcccctgc tcatagatat gcatctctaa
    23281 aaaactgttt ctgaatgcct gaatattatt ttcccttaaa tcttgagtca actgggcaaa
    23341 tctttcctct aattcttgat tttcatccaa accaatctca cctcgcctta ttcttcaaac
    23401 cgtctcacaa ctgcctccat gtctgcggct aaaggctgct tgaactccag caactcttct
    23461 gtaaatggat ggtaaaaacg cagatcataa caatgaaggg cttgtctttt tattcccaga
    23521 tccattcgcc cgccatacat atcatcaccg agcagggcac agccaattgc ggaaaaatga
    23581 actcttattt gatgtgtcct tcctgtatgc agctggatat caactaacgc gatttctgtt
    23641 agtcgcttct ctatccagta ctcagtctgt gccgaacgtc cttcatcaga gaccattcgc
    23701 ttcaatagcg aagaataatc tcgggcgatc ggcaaatcga tatgtccatg tgccgataaa
    23761 tctgaaagcc ttccactgac taaggcctga tattttttca caaacttctt ttcacgaagt
    23821 tgcatatcta attttgcgtg cgcaaatcca tgctttgcaa aaagcatcaa gccagatgta
    23881 tcccgatcta gtcgcgtcac cacatggatt acttggtttt cgtaaccttg ttttttataa
    23941 taagccttta cacgattagc cattgtccca tttggatggt attgggcagg aatagacgaa
    24001 acgcctgctg gtttatttac taccagaacg tgttcatctt catacacgat atcgatcggt
    24061 gtttcatcca aaagtacggt ttcatgttct ccttcagcag gaatcacaat ggttacctta
    24121 tcatccttag ctagagaaaa caagacattt tctacttgat cattaacttt gatttgaccg
    24181 ccttgaaatt ttattttcgc taacagaccc tttgagatcc ctttttcttt taaaaaatat
    24241 tttacttgct gagaatgatc tttctcataa tcccatgtaa actgcatatc aatcctcact
    24301 aataaaggca tctttcacac gatgccaaaa atgcatatgt cgataagaag caaaatggat
    24361 ccgttcttct gctattcgat aataaattga gcggatattt gcttttgcga cattcaattg
    24421 atcaaccgtt acaagataat cttctgtatt ttccaatttt atttctagcc attcatcatg
    24481 ggcaatcacg atcggagaac ccaaagtacg aaacactcga ttattcagtg aagcaatctc
    24541 tgccaactga aaggcattga tactaggatg gatcacagca ccgccgacac ttttattata
    24601 tgctgtcgaa ccagtaggcg tagaaatcgt caatccatct cctctaaaac tttcaaacag
    24661 ctcgtttttg atgtatacat cggctaccat cgtacgattg gcccgtttaa tcgttgattc
    24721 attcagtgct aaaaaatgct ggtccggtgt ttcatccaaa taactgatcc gaacatccag
    24781 caagggataa ctaacacttt gttcccggtt cgttcgtaag ctgtctacta gttcttccag
    24841 ctcgtaatcc cgccaatccg tgtaaaaccc taaatgacct gtatgcacgc ccaagaaacg
    24901 cacatcattc agacaatgac tgtagtgatg gaacgccgac aataatgtgc cgtctccgcc
    24961 gactgagatg accaattctg gctcatgatc atctcgttga atccctgctt gttctaaaag
    25021 tgcgttcaaa cgttctgtca cttctatagt ctttgcttct ttattatgaa cgatcgctac
    25081 tttcatcccc tgcctcctct ctttcgcttt ctttcttcaa aagcgttcga tagtagacat
    25141 catctgattt tcctcttcca tgggagaata accgctgtgc ttcttggatt tcttcacgga
    25201 tactggacat ctcttcatcc aactgatagg cagcttctgc cgcacgctga agtcgttcac
    25261 tcatttcttc tggaaaaacg ccttgatatt tataattcag cgaatgctca atcgttgccc
    25321 agaaattcat cgcaagagtc cgaatctgga tttccgccaa aattttcttc tcaccgctga
    25381 tcagctgtac aggatactcg atcaccagat gataagaacg gtagccactt gcttttttat
    25441 ttgtgatgta atctcgttct tggagaactt tcatgtcttt tcttttacgg atgatctcaa
    25501 caacttgatg gatatcttct acaaactgac acatgatcct taacccggca atatcagaca
    25561 tttcttcttc taaacgtccc attggaatat gccgtaattg tgattttgtt acaatactat
    25621 cgactggctt gactctccca gtgacaaatt caatcgggac atgtcgattt tgttcacgga
    25681 actgtttgcg gatcccccgc agtttcactt tcaattctga tacagtttgt tcatatggtg
    25741 ctaaaaatgc ttcccaatct cgttccatag ctgatacctc tacttcatta acattcattg
    25801 tttattctac catatatgca aagttttttc gtactcttga tttatgcatt gaccaaaaaa
    25861 agaaaattag gcaaaataga caaaaaggag tcgatcaaat gacacaaatg cttgaaatcg
    25921 aatataaatc tatgctgaca aagcaagagt acgagatatt catcgctcac tatcaattaa
    25981 cagataaaga tttccgtgta cagactaata tttatttcga taccgttgat gaacagctga
    26041 agaaacaaaa ttgtggccta cggatccgtt tcgttggtca tcaagctgaa tatacattaa
    26101 aaacacctgc tgaaaaaggg cgtctcgaaa caacggatac ctttataaca gaagaagcag
    26161 aagcatttat cttcgaaaaa cgtctgcctc gttcaggcgc agtttttcaa aaactttctg
    26221 atttgaatat cgatcctgct tcattaatcc aaaccggaaa gcttacaaca aaacgagctg
    26281 aattccctat tgaagaagga cttttagcta ttgatgaaag ctggggagac aatctacacg
    26341 attttgaact ggaactagag gtaggggatg catctgtggg aaaaattgct tttataaatt
    26401 ttttaaaacg tttttcttta ccttatcgtc ctgctaagaa caagatccaa cgtatgcttg
    26461 aagctaaaaa ttagaaacct ttcattttca caaggcactt gagttcattt ttttactttt
    26521 ttctgttaag ttataggatg tgaaagaatc tagtcactag ttgtatagaa tctattttgg
    26581 gggagattca tatgatcgaa atctatttat ttgttaatcc tttaggggaa tactgtttcg
    26641 aaatggaaca acagttatta caatttatcg aagaagagta cgggacacca tcaaaagaaa
    26701 agatgcaatt tcgctttctt cctttggtca atttgcagac aattggggat gtcatgcaac
    26761 aaaaaggaat ttctcagaac aatcttgagg aacgtaatca cttattctca atgacttatt
    26821 ctgcagcatt ggactgcaaa gcggcgcagt ttcaaggaaa gaaaaaaggc cgccgttttt
    26881 tgatcaaatt gcaagaagca gtcggctgta atgacatacc atattctcaa gaacttgctg
    26941 aatccatatt tgaagaaatc ggcggcgatt tgaatatgtt taaagaagat cgtcagtctg
    27001 acttcgttaa agaagtattc ttttctgatc aaaaaattgc tcgggaaatg ggcatcacaa
    27061 aacacccttc tgcagtcgtt tacaattatg cctgcgaccg tgattttggt gttttagtag
    27121 aagtaaaagg tacaagtagc ctttggagaa tcacagagct gtgtaaaaca acagataact
    27181 catatcaagt ttttaatggt gaacatttac atacaaatga ttctcagttc cattcaagaa
    27241 ctagccattt acacttgctt tctaattaaa aa
//
LOCUS       DANKEF010000033        26813 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000033 DANKEF010000000
VERSION     DANKEF010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26813
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.033"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..411
                     /locus_tag="PWZ48_001638"
     CDS             <1..411
                     /locus_tag="PWZ48_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2261296.1"
                     /translation="GLYYSPVSTPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAP
                     QEYLSRAYNDAPKYCANIVNTQLKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLA
                     ELKGYQVTTGEPVCQTRRLNSRIDKVRYMFNKIT"
     gene            509..697
                     /locus_tag="PWZ48_001639"
     CDS             509..697
                     /locus_tag="PWZ48_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL2261297.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(755..1108)
                     /locus_tag="PWZ48_001640"
     CDS             complement(755..1108)
                     /locus_tag="PWZ48_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261298.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1639..2061
                     /locus_tag="PWZ48_001641"
     CDS             1639..2061
                     /locus_tag="PWZ48_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2261299.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2058..2288
                     /locus_tag="PWZ48_001642"
     CDS             2058..2288
                     /locus_tag="PWZ48_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261300.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2786..2986
                     /locus_tag="PWZ48_001643"
     CDS             2786..2986
                     /locus_tag="PWZ48_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2261301.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3013..4206
                     /locus_tag="PWZ48_001644"
     CDS             3013..4206
                     /locus_tag="PWZ48_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2261302.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="PWZ48_001645"
     CDS             complement(4274..5839)
                     /gene="guaA"
                     /locus_tag="PWZ48_001645"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDL2261303.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5898..6821)
                     /locus_tag="PWZ48_001646"
     CDS             complement(5898..6821)
                     /locus_tag="PWZ48_001646"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDL2261304.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            7017..7622
                     /locus_tag="PWZ48_001647"
     CDS             7017..7622
                     /locus_tag="PWZ48_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2261305.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7930..8946
                     /locus_tag="PWZ48_001648"
     CDS             7930..8946
                     /locus_tag="PWZ48_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2261306.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            9087..10388
                     /locus_tag="PWZ48_001649"
     CDS             9087..10388
                     /locus_tag="PWZ48_001649"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HDL2261307.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            10434..11096
                     /gene="deoC"
                     /locus_tag="PWZ48_001650"
     CDS             10434..11096
                     /gene="deoC"
                     /locus_tag="PWZ48_001650"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDL2261308.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            11117..11515
                     /locus_tag="PWZ48_001651"
     CDS             11117..11515
                     /locus_tag="PWZ48_001651"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDL2261309.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11581..12663
                     /locus_tag="PWZ48_001652"
     CDS             11581..12663
                     /locus_tag="PWZ48_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2261310.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12875..14437
                     /locus_tag="PWZ48_001653"
     CDS             12875..14437
                     /locus_tag="PWZ48_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2261311.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            14430..15563
                     /locus_tag="PWZ48_001654"
     CDS             14430..15563
                     /locus_tag="PWZ48_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261312.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15563..16519
                     /locus_tag="PWZ48_001655"
     CDS             15563..16519
                     /locus_tag="PWZ48_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261313.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16618..17784
                     /gene="deoB"
                     /locus_tag="PWZ48_001656"
     CDS             16618..17784
                     /gene="deoB"
                     /locus_tag="PWZ48_001656"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDL2261314.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17808..18626
                     /locus_tag="PWZ48_001657"
     CDS             17808..18626
                     /locus_tag="PWZ48_001657"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2261315.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18643..19350
                     /gene="deoD"
                     /locus_tag="PWZ48_001658"
     CDS             18643..19350
                     /gene="deoD"
                     /locus_tag="PWZ48_001658"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDL2261316.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="PWZ48_001659"
     CDS             complement(19648..20334)
                     /gene="gpmA"
                     /locus_tag="PWZ48_001659"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDL2261317.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20506..21087)
                     /locus_tag="PWZ48_001660"
     CDS             complement(20506..21087)
                     /locus_tag="PWZ48_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2261318.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="PWZ48_001661"
     CDS             complement(21220..21903)
                     /gene="rpiA"
                     /locus_tag="PWZ48_001661"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDL2261319.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            22222..22635
                     /gene="rpsL"
                     /locus_tag="PWZ48_001662"
     CDS             22222..22635
                     /gene="rpsL"
                     /locus_tag="PWZ48_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDL2261320.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22719..23189
                     /gene="rpsG"
                     /locus_tag="PWZ48_001663"
     CDS             22719..23189
                     /gene="rpsG"
                     /locus_tag="PWZ48_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDL2261321.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            23283..25370
                     /gene="fusA"
                     /locus_tag="PWZ48_001664"
     CDS             23283..25370
                     /gene="fusA"
                     /locus_tag="PWZ48_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2261322.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25542..26729
                     /gene="tuf"
                     /locus_tag="PWZ48_001665"
     CDS             25542..26729
                     /gene="tuf"
                     /locus_tag="PWZ48_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDL2261323.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
BASE COUNT         8434 a         4951 c         5684 g         7744 t
ORIGIN      
        1 ggtttatact atagccctgt tagtactcca gcagattttc ggatgcttac tcctattgta
       61 ctggagcaag cctttagaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 gtttatgcac cacaggaata tctttcacgg gcatataacg atgctcccaa atattgtgca
      181 aatatcgtaa atactcaact gaaaaataat gaggtcatta ttattggaga aattcctgct
      241 cgatgtattc aagattatcg caatgattta acttttttta caaatgggct tagtgtttgt
      301 ttagcagagc taaaaggata tcaggttacc actggcgaac ctgtttgcca gacccgtcgt
      361 ctaaatagtc ggatagataa agtaagatat atgttcaata aaataactta gtgcgtttta
      421 tgttgttata taaatatggt ttcttattaa ataagatgaa atattcttta atatagattt
      481 gaattaaagt ggaaaggagg agattgttat tataaactac aagtggatat tgtgtcctat
      541 ttgtggaaat aaaacaagac tacgaatacg agtggatact atacttaaaa atttcccttt
      601 atactgcccc aaatgtaaga acgaaacttt aattaatgtt caaaaaatga atataataac
      661 aatcaaagag ccagacgcca agacgcagag ccgataattt gagaaatgaa actctcatct
      721 tatcggctct ttttgtttat ctgaatttta ctgactagcc ttcaatattt cttgcttcat
      781 tgataccttt tgctagtgct tccattaagg atagttcttt gtctgtaaag ctatccatgt
      841 atttttctac ctgtaacctt cgagtgcttt taatcaaatt atctgtaggt aagaaaaatt
      901 catcaataga tacatggagt aatgatacaa gatcatacag cacttgtgta cttgggtgtt
      961 gtcctttgtt ttctatattt gttaaataac gtgggtcaat ttcaatcatt gttcccactt
     1021 gttcacgagt gagaccccgt ttcattcgag cctctttaat cgctaaacca acggctctaa
     1081 aatcgtattt atcttctttt ttacgcatac taaatcacct ctatacattt tactgttcct
     1141 atataaccat aaacaggtat agaaaaacat gttatatagt ttatgagttc ctatagaaca
     1201 ctgtggaatt tattaatgcg atgaatccaa tttcaaatag aaaggtcgct gattttattt
     1261 acctatacaa ctttataaaa tttagtcata ctcctagaaa gtagataaaa ttggcgactt
     1321 aatggaggga aaagtcgcca tgagaaagat aatctgtcat ctagttctct taattaagtt
     1381 cttatcaaaa aaacttatcc acctgtactg gatagatatg accatcaatg tggttttgca
     1441 gattacatag atagaaaaat caaagcatat gaacagagaa aacaatctgt ttatgtgctt
     1501 ttttggtcac ttaataaata tcttttagga tttctcttca tttatgcaac aaatcccccg
     1561 ttcgtgtcgg ggtttagtat gaagggagat aaagaagcac atgccactcc ctttcacgac
     1621 agaaaggggg tgaaaagcat gaaaccatct tcttttcaag aaaccataga aaatcagttt
     1681 gactatatct gcaaacaagt tattgaagat gaacgaaaag attacttcaa acatctagac
     1741 agactatcag caaaagaagt ctcgttttct gatatagata actaccttgt caactctttt
     1801 tctactgttg atacgtacgt cactgatttc caactattta aattatttgg attaagaatc
     1861 aatgttgaaa gtgatttatt aagtgaagcc ctgcgtcatc tttcagagaa aaaacggaac
     1921 attattttac ttcattactt tatggatatg agtgatgtgg agattgcgga gttactaaat
     1981 ttgaatcgtt cgactgttta tcgtcatcga ataagtggac tagccatgat taaagaattc
     2041 atgaaggagt gtaaagaatg aaaacagctt atccaagggt tcccttcccc ctcattgtaa
     2101 aggcgacaga tggcgacgtg gaagcaatta atcagattgt taaacattat cgagggtata
     2161 cctcaaaacg ttcattacgt cgcatgacag acgaatatgg aaattctcat atggttattg
     2221 atgaaaccct acgtggcaga atggaaacga aactcatctc taagatttta tcgttcgaaa
     2281 ttaaatagtc ccattttttc gtggaagcgt gtcgaatcgt atccacgctt cccgatttgg
     2341 gaggtttgtt attccatcaa agcatattgt gtgttcagta ggttttgata ggctaacaag
     2401 ccaaacgttc tttgacaacc aaataaaggc aatcgaatac gtttcggtaa gaaaagagct
     2461 gatgaactaa cacgccatga cctacaaaat tagtgagcga ttcatgttag taattcgaaa
     2521 gtaatcttta gcaggattgc tgccatgact ttcttatcgt gataatgata ctcccgtata
     2581 gccatagtcc gagcgttaga agcgtcacag gcaatgggta cggctacatg agaaccatgc
     2641 agaagtgaaa ttcctgtgag ctttgctaga gctgttcgat tgctaagata ttcttctctt
     2701 gtcagtgtat tattataaaa ctatttgata taattatagg tgaatattgg agttttccta
     2761 gcctataatt gggaaaggag taataatgat aacggtcaca aaaaaggatt tagttgagct
     2821 aggatatgga acttcttttt cagcagacat cataaaaaaa gcaaaagaat tgatgattac
     2881 aaaagggcat atctactatc aatctcgaaa gctagataga gtccctcgtg aagctgttga
     2941 agaaattcta ggaattcggt tttcaaatga aaacaggtct gagtagtttt tgcacagtaa
     3001 ggagtaaaaa ctatggttaa agacccaata aaaaaagcaa agaatggaac ctattatttt
     3061 cgtgcaaatc taggttatga ttctaaaggg aaaaaaattc aaaaatatcg tagtggattc
     3121 actactaaaa aagaagcaag agaagaatat tcgaaactgc tattgatgaa accagaagaa
     3181 ttaagtgaga ataaggataa aatgacgttt aaacattata tctttgaaat tttccttcca
     3241 tggtataaga cacaggtaaa gctcagaaca tatgaaaatc gcttaccaac tattaagaag
     3301 catttctctt attttgataa aatggctgtt tccgatattg agcctattga tgtgcaaaat
     3361 tggcaactaa aattatcaaa gaagtgtaag tcttcttatg taagagctgt tcaaggacta
     3421 ttttcagttg ctatggatcg agcaattgtc cttgggataa ccaccacaaa tccatctaag
     3481 attattggca atgtgaaaaa gcaaaagtct aaaattgaat tttggacaaa agaagaattt
     3541 gaaaaagtaa tctctctaat ttacaaagaa gattactatc aacacttttt atttatctct
     3601 ctgtggtttt tatttatgac tggaatgcga attggtgaag ctactgccat tcaatgggaa
     3661 gatattgatt ttgactcagg agtactaacc atcaacaaaa cactctatta caagaatcaa
     3721 tcgaattata gatttgttga accaaaaaca aaggcgagcg ttcgtcatat tgtattagat
     3781 aaatgcacat taacttattt atccgaatgg aaggaagtac aacaaaatct aattcagaca
     3841 gactttgtaa tgagctataa cggtattcct acccaaaaac atacactatc atatgctatt
     3901 gaacgctatg ctaagctggc tggtatccat cgaatcagaa tccatgggtt acggcattct
     3961 catgcttcct tgttaatcag tatgggagaa aatccactta tcattaaaga tcgtctagga
     4021 cacgaagata ttgagacgac tttaggaacg tatggacatc tttatcctaa cagtaatttc
     4081 gaggtagcta acaaattaaa tggagccatt tcctttaaag aagcaaaaca aaattatgat
     4141 acttcaccaa agaatcaatt tactgtagac tacttgcgaa acaaagaaat gaaaaaagtg
     4201 caataaaagt gcagtaaaaa gataagaaga gccagaaacc cttggtataa gaggatttct
     4261 agctctccta caactattcc cactccactg ttgctggtgg tttagaagtg atgtcataca
     4321 cgatacggtt gacgtgggct acttcgttta cgatacgtac agagattttt tgcaagactt
     4381 cccatgggat acgggcaaag tcagcggtca ttccgtcgat tgaagttaca gcacggatac
     4441 cgactgtgta gtcgtaagta cgtccgtcac ccataacacc gacagagcgg atccctggaa
     4501 gaacagtgaa atattgccag atatcacggt caagtccagc agctgcgatt tcttcacgca
     4561 agatagcatc agattcgcgt acgatttcta gcttgtcttc agtgatttcg ccaagaacac
     4621 gaataccaag accaggacca gggaatggct gacgccaaac gattgaatca ggcatgccta
     4681 actgagtccc taattcacgg acttcgtctt tgaataatgt gtttaatggt tcgatcaatt
     4741 caaattgcat atcttctggc agtccaccta cattatggtg agatttgatc gtctgtgctg
     4801 tttctgtacc agattcgata acgtcagtat acaacgttcc ttgtgctagg aaagagacgc
     4861 cttcttcacc agcaagtttt gttgcttcgt catcaaaaac atatacgaat tcattcccaa
     4921 tgattttacg tttttgttct gggtcagata cacctttcaa cttgtctaag aagcgatctc
     4981 ttgcatcgac tttgatgatg ttcaagccga attttccgcc tagactttcc attacttgtt
     5041 ccgcttcgcc tttgcgtagc agaccgtggt caacaaagat agatgtcaat tggtcaccga
     5101 tcgctttttg taaaagaacc ccaacgacac tagaatcaac gccacctgaa agaccaagca
     5161 agactttttt gtcaccaact tgttcacgga ttttttctac ttgcatatcg ataaagttat
     5221 ccattgtcca gttaccttca cagtgacaaa catcaaaggc aaagtggcgc agcaattcga
     5281 ttccgtattc ggaatgacgt acttctgcat ggaattggat accataaaaa ttacgtgttg
     5341 tgtcttggat agaagcaatc ggacaatctt tgcttgttcc gactgtttca aagccttcag
     5401 gaactttagt taccaagtca ccatggctca tccatactgt ttgttgtttt ggtgtttctt
     5461 taaacaagac agcttgatcg cttgttactt ctaacaaggc tttcccatat tcgcggtttt
     5521 tagcaggctc tacggaaccg ccaagtttgt acgtgatcaa ttgcatacca tagcaaatcc
     5581 ctaatacagg aatacctaaa ttgaagatct caggatcgat cccaaatgca ttctcatcat
     5641 atacactgtt tggtccgcca gaaagaatga ttccctttgg attcatcgct tttacttctt
     5701 ctgcagtaat gcggtggctc aataattcgg aaaagactcc aagatcacga atacgtcttg
     5761 tgatcaattg gttatactga ctaccgtagt ctaatacgat aatcttttca acgttcgtca
     5821 tatcggcaac gtttgtcaaa tttattaccc ctatcccttt aaattttatc tcaaaatagt
     5881 caaactattt taaaatgcta atattttcgc atgaaaatcg aatcgatgat atgattttca
     5941 gttttatgga gaattatatc ggctcttcct cgtgtaggca agatatattc ctgtaaattt
     6001 ttcaaattca catttttcca tacatctttt gccattttaa aggcttcttc acgatctccg
     6061 atagcaaaac gataataata attctctgga ttttggaaag ccgtatccag taacgcacca
     6121 aaacgatcca aataccattt ttcgatcagc acgggatcgg catcgacata gacagaaaaa
     6181 tcaaaaaagt cgctgacgta gatttgctga ttcgcaggta attgaagcgt attgatcccc
     6241 tcaacgatca agatatcagg ctgctggatc aattcgtact tgtctggaat gatatcatac
     6301 acatcatgtg agtaaacagg tacttttatc tcttcctgac cagattttac acggttcaag
     6361 aaatcgatca gcatctccat atcataactt tcaggaaatc ctttacggtc catgattcca
     6421 cgctctttta atatacgatt tggataaaga aatccgtcag tcgtgatcag ctgaacgttt
     6481 cttcgtttga aggtacgaga caaaatcgtc tgcagcagtc tggctgttgt actttttcca
     6541 acggctacac ttccggcgat cccgataata aaaggcggta cttctacgta tttatgaaga
     6601 aataaacctt tactcaagaa caaagattca aattctttca tataaagata aatcagatga
     6661 gcaagaggta catagatatc tcgaacatcc tgcatggaaa tgcggtcatt caaacttttg
     6721 atattatcta gttcatcttg tgtcaaaggt gcttgcccat tacgataaaa ttctcgccat
     6781 tcttctcttg ccacacggta gtagttcatt ggatcattca tattagcctc ctgcgttctc
     6841 ccatcaatgg aactatttta acacaaaata aacgcaaacc ccaccactta agagagattg
     6901 ccgatctttt tgtataatct ttataataga aagaaacgga gtcttattct tgacttgcgc
     6961 acggatacat aaaaggatag actgttgtca tgaaagaaat gagaaggatg gaagacatga
     7021 caaatcatta ctattctgaa cgcccagata cggcgcacga ttttgaacaa tgggcttttg
     7081 agttaaaagg cagaaccttt caatttgtaa cagattctgg tgtattttca cgtgaaacag
     7141 tggattatgg ttcacgtgtc ctgattgatg ctttcgattg ggaagagtta cctgatgaag
     7201 gaaagatctt agatgtggga tgcgggtatg gaccgattgg gttagctttg gcatttgcga
     7261 cacagcggtt tgttgaaatg gtcgacatta atgaacgagc ggtaagtcta gcgcaaggaa
     7321 acgcaaagcg taaccaaatc gaacatgttg atatccatca atcgaatatc tatgaagcag
     7381 ttcatgaaga aacctatgca gctatcgtaa gcaatccgcc gatccgtgct ggaaaaaaag
     7441 ttgtacatga aattttgaca ggcgcatatc ctcggttgaa gaagggcggt acactgacaa
     7501 ttgtgatcca gaagaaacaa ggagcgccga gtgcgcaaaa gaaaatggaa gaaacatttg
     7561 gtaatgcaga aatcgtcaca aaagataaag gatattatat catcaaaagt gtgaaagaat
     7621 aactagctga aaaatagtgg agcgacgaaa gagtggaact gggcgtgcat gtacgggtac
     7681 gtatcagttc tttttttgtt gagaagtttt taaaaacaaa gaactatttt ttcttcaaaa
     7741 agaaatagta ggaacagagt cgttgttttt tacgataaaa taaataagat acagaacttg
     7801 tatagaaggc tgccaagtcg gtagataaag gaggttttta tcaaaaaaca tactttcctt
     7861 ttttctctat tcttttcttg ggggttttgg tacactgata gggaatttag atagaaggga
     7921 gccaatagga tgaagctgac catcagagat atcgcagaaa tggcaggcgt atcagtaaca
     7981 actgtctcac agattttgaa taacaaagga agcagattta gtgataagac aagaaaaaaa
     8041 gtgttgtcga tcgtgaatga acatcattat aagcccgatt atttcgcttc aaatgtgatc
     8101 aatcgacatt caaagaccat cggaatgatc gtccctgatg tcactgattt cttcttctcc
     8161 aaactgatcg aaggtgtaga gagctatttg aatcctttgg gctatgtgat catgttatgc
     8221 aattctcgtc atagccaaga aaatgagatc aagtatttac aagagctttc ccaccgctca
     8281 gtggacggaa tcttgctggc cacaccaaat attttacctg agaaatatgc attagacagc
     8341 ggtttttatc aaaaaatgcc aatcatttta atcgatcgcg gattgaatcg tcgggataac
     8401 ggacggctta tcgtaaagga atatgagggc gcctatcaag ctgtttcgct gtttattgaa
     8461 aatggccata cgaagatcgg catgctgaag gaaaccactg gatactatca attagaagaa
     8521 cgtttcaacg gatatcgtca tgcgctgaaa gatcatggcc tgccttttaa tgggaaattt
     8581 gttgaacatg gagagttgac tgtccaaggt ggatatactg catcaaaaaa attactcaaa
     8641 cataaagata tcacggctat tttttgcggg aatgatgcca tggcgatcgg atgctatcag
     8701 gcgatcgatg aattgggcaa aaaaatccca gaagatatct cagtcatagg attcgatgga
     8761 ttaaagctgt cagaatacat gattccgcaa ttgacgacag tgcagcagcc aagttttgat
     8821 attggatttt atgctgcacg tttcctgatc gatacgatcg aatttcctca aagaaaagtt
     8881 ccgaacaaag tgttcgaaac aaaattaatt attcgtgaga gcgtaaaagt attgacaaag
     8941 ggctaactcg gcggtatatt gcttatgtaa gcatttacaa aggctagtta gtgctttttt
     9001 tatttttagt aaaccagttt actaaagtgg tttacctttc ctaaaaataa tgtataatac
     9061 cgaatgaaga caaggaggat caggcaatgc gaatggtgga tctgattgaa aaaaagaggg
     9121 atggaaacga gctgtcgaaa gaggaaatcg agtacatcgt cacgaactat acgaatggaa
     9181 agattccaga ttatcaagtc agtgcattat tgatggcaat tttttatcaa gacatgacaa
     9241 atgaagaaat cacgaactta acattggcga ttgctaattc tggtgatgtc atcgatttga
     9301 gttctcttga aggaataaag gtagataagc attcgacagg tggcgttggt gacacgacca
     9361 cattgatttt agccccccta gtagctagtg tcggagtgac agtggctaaa atgtctggac
     9421 gcggtctagg ttacacaggt ggtacactag acaagctaga agcaattccc ggatttcaga
     9481 tcgagctttc ggatgaagaa tttgtacgca ttgttaatga aagtaaagta gcggtaattg
     9541 gtcaatcagg aaacttggca ccggcggaca aaaaattgta tgccttgcgt gacgtcacag
     9601 cgaccgttga ttctcttccg ctgattgcca gttcgattat gagtaaaaaa atcgcggcag
     9661 gagcagatgc aatcgttttg gatgtgacca ctggagacgg tgctttcatg aagaacatcg
     9721 aagatgcaag acgtctggca aagaccatga cgagcattgg gaaactggct aaccgagaaa
     9781 cagtggcggt tatctcagat atgtcagaac ctcttggcga agctatcgga aatagtctag
     9841 aagttgtcga ggcaatcgaa acattacaag gaaatggtcc agaagatcta gtagaaatgt
     9901 gctacgcctt aggaagccaa atggttgttc ttgctggcaa agctaagaca accgatgaag
     9961 caagaacgtt gcttcaagaa gctctggaat caggtaaagc gttagcaaaa ttcaaagaaa
    10021 tgatccaaaa tcaaggggga gacccagcga tcgtggaaca tccagaacga attttgactg
    10081 cacgctacac aatggagctt cctgctaaac aaagcggagt tgtgtcaaaa atcgtggcaa
    10141 atgaattagg aattgctgca atgatgctag gtgctgggcg taaaaccaaa gaagaagata
    10201 ttgatcacgc tgttggattg aaactgcata aaaaaattgg tgacactgtg acaaaaggtg
    10261 agtcactatt gacgatctat agtaacgacg aagaaatcac ttctgtcatt gaattattat
    10321 ataaaaatat tgaaattggt gaatcggcaa tgaagccaac gttgattcat gacatcatca
    10381 cagaataaag gaaaaaagcg gactttaccg ttttaaagaa aaggggaaaa gaaatggaac
    10441 taaaccgaat gatcgaccat actattttga aagcagacgc atctaaagaa gctgtgatgc
    10501 aaattatcga agaagcaaaa aaataccatt tttattctgt ctgcatcaat ccagcatggg
    10561 ttgcgctagc gaaagaacaa ttacaaggtg agccagtagc cgtatgtaca gtaatcgggt
    10621 ttcctctagg agcaaacaca cccgagacaa aagcatttga aacaacagat gccatcaata
    10681 acggtgcaga tgaagtcgat atggtcatca atatcggtgc tttgaaatca aaggatgatc
    10741 aacaagtcca aaaagatatc gaagcagttg ttgaagcagc aaaagatcgt gcgcttgtca
    10801 aagttattat tgaaacatcg ttattagata gagaagaaat catccgagct tgcgaaatag
    10861 caaaagctgc aggcgctgat tttgtcaaaa catcaactgg tttctcaaca ggcggagcaa
    10921 aggtagagga tgtccgtttg atgcgtgaaa ctgtaggacc agaaatgggt gtcaaagctt
    10981 ccggcggtat ccataacgaa gaagaagcaa tggcaatgat cgaagctggt gctacaagaa
    11041 tcggaacaag cgcaggagta gcaatcgtat ctggttcaac tggtgaaggc tattaatttt
    11101 ttcaaaggag aagctcatgg cagaactcaa acaagaatgg atcaacacag ctattgaagc
    11161 gttagataaa gcctatgttc cttattccca ttttccggta ggcgcttgtc tcgtaacgga
    11221 atcaggcaag atttatcaag gcatcaacat tgagaatgca tcatttggat taacgaattg
    11281 tgccgagcgc acagcttttt tcaaagcagt atctgaagga gaaaaaacat ttacgcatct
    11341 agtcgtagcg gggcacaccc ccgatcctat ctctccttgt ggtgcatgtc gacaagtaat
    11401 ggcagaattc tgttcccctg atatgccggt tacgctagta ggagacaatg gtgtgacgaa
    11461 ggcaacgacc gttcgtgcac tgctgcctta tgcatttact gaaaaagatc tataactcat
    11521 gttcattcag tcagctgact gttgaaaaat aaaacaatta ttttaggagg ctttacagaa
    11581 atgaaaaaag caaaattatt tggtttaggt gctgtcgcac tagcagcagg gttattttta
    11641 ggtgcatgcg gcaacaacgg ctctactgat tcatccggtg gcaaaagctc atcagacaca
    11701 acaactgcag cattgatcac agacacaggt ggtgtagacg accgttcatt caaccagtca
    11761 gcttgggaag gactagaaaa atgggggaaa gatcaaggac tttcacgagg aaatgatggc
    11821 ttccaatatt tccaatcttc aaatgaatca gattacatcc caaatattga ccaagcattg
    11881 aatgctggat tcaaaacgat ttttggtatt ggttataaac taaaaccagc gatccaagaa
    11941 caagcaacaa acaatacagg tacgaacttt gttattatcg atgatgtcat cgatggatta
    12001 gataacgttg tatctgcaac tttcaaagac aacgaagctt cttatcttgc aggtgttgca
    12061 gcagcttata cgactgaaac aaacgtagtc ggctttatcg gcggtgtaaa aggtgaagta
    12121 atcgatcgtt ttgatgctgg attcaaagcc ggtgttgatg caggtgcgaa agaattagga
    12181 aaagaaatca aagtattgaa tcaatatgct ggtgacttct ctgctccaga taaaggacgt
    12241 tcaattgctc aaggtatgta tgcacaaaac gcagatatca tcttccatgc ttcaggcggt
    12301 acaggtaacg gtgtattcca agaagcaaaa tcattgaacg aatcaggcga caaaaaagtt
    12361 tgggttatcg gtgttgaccg cgaccaatca gacgaaggcg aatacacatt aaacggcgaa
    12421 aagaaaaact tcacattgac atctacactg aaagcagttg gaacagtagt agaagatttg
    12481 gcacaaaaat cagcggacgg taaattccct ggcggcgaac acactgttta tggattgaaa
    12541 gaagacggtg ttggtctaac agaaggacaa ctttctgacg aagcgaaaaa agcagttgat
    12601 gaagcaaaag aaaaaatcat ttcaggtgac gtaaaagttc cagaaactcc tgaagaaaac
    12661 taaaaccatt tctatagcct tgttagacac atgtttgaca aggctatggt ttgattagaa
    12721 caagaattgt tcatttatcc aagctgcctt gtgacagttt ggataaggga acaactccct
    12781 gaagtaatgt ttgtttcaaa agaagaacgc tcactaagcg tgaatcttag tcaatcgttg
    12841 ccctagatag aaatcgttag gatgtgaaaa aaaggtggtt caagaaaata ttgtcataga
    12901 aatgcagaat attaccaaag aatttggaac attcaaggca aatgacaaca tcaatttaca
    12961 agtaaaggca ggagaaattc atgcgttgct tggagaaaac ggtgctggca aatctacatt
    13021 gatgaacgtg ctttccggat tattagagcc gacatcaggg aaaattttga tgcgtgggaa
    13081 agaagtaaag atcacaagtc cgaccaaagc caaccaatta gggattggga tggtccatca
    13141 gcactttatg cttgttgatg cctttactgt aacagaaaac atcgtgttgg gaagcgagcc
    13201 tagtcgcgca gggatgcttg accataaaaa agcacgaaaa gagatccaaa aagtttctga
    13261 acagtatgga ttgtcagtca acccagatgc ttacgttcgt gatatttcag ttggaatgga
    13321 acaacgggta gaaattttaa aaacacttta ccgaggagca gatgtactga tttttgatga
    13381 gccgacagct gtattgaccc ctcaagaaat tgatgaatta atcgtgatca tgaaggaatt
    13441 agtcaaagaa ggcaagtcga tcattttgat tacgcataag ttagatgaaa tcaaagcagt
    13501 agctgaccgc tgtacagtta tccgccgtgg aaaaggaatc ggtacagtca acgttaaaga
    13561 cgtaacctca caacaattag ccgatatgat ggttggaaga acggtttcat tcaaaacgat
    13621 gaaaaaagaa gcgaagcctc aagaagtcgt tttgtctatt gaaaatctag tggtgaaaga
    13681 aaatcgtgga ttagaagccg tgaaaaactt gaacttagag gttcgtgctg gcgaagtact
    13741 tggtatcgct ggaatcgatg gaaacgggca gtcggagttg atccaagctt tgactggttt
    13801 gcgaaaggca gaaagcggac atatcaagct aaaaggggaa gacatcacta ataaaaaacc
    13861 ccgaaaaatc actgaacatg gtgtaggaca cgtgccagaa gaccgtcata aatacgggtt
    13921 agtcctagat atgacattat ctgaaaatat cgcactacaa acctatcatc aaaaacctta
    13981 cagtaaaaac ggcatgctga attattcagt aatgaatgaa catgccagag aattgatcga
    14041 agaatatgat gttcgaacaa cgaatgaact tgttcccgca aaagctttat caggcggaaa
    14101 tcagcaaaaa gcaattatcg ctcgtgaagt cgaccgagat cctgatctgt tgattgttgc
    14161 aaatccaact cgtggactgg atgtaggtgc gatcgaattt attcataaac gtctgatcga
    14221 acaaagggac aaatacaaag cagtgttact gatcagtttc gaattagaag aaattttaaa
    14281 tgtttcggat cgtattgctg ttatccatga aggagaaatt gtcggtatcg ttgatccgaa
    14341 agaaacatct gaaaatgaat taggtctatt gatggcgggc tataccttag aagaagcacg
    14401 aaaagagtta gaaaaggggg ctggcgcaca tgaatgatcg cagcgaaaaa ttccgtaatt
    14461 tattagtgcc tgtactttca gttgttttag gattgatctt aggcgcgata ttgatggctg
    14521 cttttggttt tcagcccatc caaggttatt cttctatgct agatgcagcc cttggttcgc
    14581 aaagagggat aggtgaaacg ttaaggcaag caacgccgtt gatttttaca gcattaggtt
    14641 tttctgtagc taactcagct gggtttttca atattgggct ttctggacaa gcactttgcg
    14701 ggtggatcgt cagtgtctgg gtggcacttg cgtttccaga tctgccaaaa gttgttttgc
    14761 ttccgttatg cgtgatctta ggtgccttag ctggtgcgat ggcagcggct gttccaggtt
    14821 tacttcgagc attttttgga acaagtgaag tgatcgtaac aatcatgatg aactatatct
    14881 tattatacgt aagcacattt gctttacatg aagtgatgcc tgaaagctat cgctcaagtt
    14941 tggactcttc caatatgatc agtcaaaatg cctcactgcg gttaccatgg ttaacacaag
    15001 cttttggcgg ctctcgcgta aactcaggat tatttttagc aatcatcgcg cttattttcg
    15061 tttggctgct gatgaagaaa acgactttag gatttgaaat ccgttctgtt ggattgaatc
    15121 cttttgcttc tgagtatgca ggaatgagta gtaaacgtac gatcgttttg tcgatggtca
    15181 tttctggcgc tttggcaggg cttggcggtg tggttgaagg attaggaacg tatcaaaact
    15241 tctttgttca aacgacttcg ctatctattg gatttgacgg aatggctgtt tccttgcttg
    15301 gttctggatc agcaatcggt attcttttat cagcgatctt gttcagtatt ttgaagatcg
    15361 gtggacttgg catgcagaca ggagcaggtg ttccgtttga gatcgtcaat gtatcgattg
    15421 ctttgattat cttctttgtc gggatcaact tcctgattcg tttcgtcatg gctaaagtct
    15481 tccaagggaa aaaacaagaa gagatcgttg cgacaataga atcgaaacca acgaatcaaa
    15541 accatgaagg gggcgaacta taatgtcgac tatcgaatta attgccttga tccttacttc
    15601 tacactggtt tattcaacac cgctaattct aacgtcacta ggcggaactt tttctgaacg
    15661 aagcgggatc gtaaacgtag ggctagaggg aatcatggtc atgggtgctt ttagttccat
    15721 cgttttcaac ttgacgatga gtccatcact tggttctatg acaccatgga tcgctatttt
    15781 agtgggtggc gtcgtaggta tcctattttc tctgattcac gcagtagcaa cagtcagttt
    15841 gcgtgctgac catattatca gcgggacagt aattaatctg atggcacctg ctttaggtgt
    15901 tttcttgatc aaagcatggt atggtaaagg tcaaactgat aacatcacgc agaatctggg
    15961 gtatttttct ttcccaggat tagcagatat tccggtcatt ggaccaattt tctttaaaaa
    16021 tacattctta cctgcttatt gtgctatttt gattgctgtc ctagcttggg tgatcatctt
    16081 caagacgaaa ttcggcttgc gcctccgttc agttggtgaa aatcctcaag ctgccgacac
    16141 attaggtatc aatgtatatg ctatgcgtta ttcaggcgta ctgatttctg gcttgttagg
    16201 cggtatgggc ggtgctgttt ttgctcagtc gatttccggt aacttttcag ctggtacgat
    16261 cgttggacaa gggttcatct ccatggctgc tatgatcttt ggtaagtgga atcctttagg
    16321 ggcaatgggt gcctcattgt tctttggttt tgctcaaagc ttgagtatca ttggcgcaca
    16381 attaccagtt atttcttcga ttccgccagt attgctgcaa attgctccat atttgctgac
    16441 aatcattgtg ctggttgttt tcttagggaa agcttctggt ccaaaagcaa acgggaagaa
    16501 ctatatcaaa tcaaaataat cttttagcga ataaaaaggg tgggttacgg gaagtgtttt
    16561 tcgtctcgcc ctttttgttt taaaggcaat aaaaataaaa agaaagaagg aataatcatg
    16621 tttaaacgcg tacatctgat cgtcatggat tcagtaggaa ttggagaagc accagatgcc
    16681 gataagttcg gtgatgttgg ttctgataca ttaggacaca ttgcgaaaga agcaggattg
    16741 aacgttccac atttagagca gcttggttta ggaacgatcc gtcctcttga aggtgtggaa
    16801 tcagtgaaag atcatcaagg ctatgcgacg aaattagaag aagtttctgt agggaaagat
    16861 acgatgacag gccactggga aatcatggga ttgaatatca aaactccatt tcgtgtgttt
    16921 cctaatggat tccctgatga tctgatcaaa caaatcgaag aattttctgg ccgtaaagtc
    16981 gtatgtaaca aaccttatag cggaacagct gttattgacg attacggtga acaccaaatg
    17041 aaaacaggcg atttaattgt atatacttct gccgatcctg tccttcagat cgcggcacat
    17101 gaagaaatca ttcctttaga agaattatat cggatttgcc aattcgttcg tgatataaca
    17161 aaagatgatc cgtatatgat tggccgaatc attgcacgtc cgtacgtggg tgaaccagga
    17221 gacttcacac gaacaagcaa tcgccatgat tatgcgttga atccattcgg acacacagtc
    17281 cttgattctt tgaaagaagc aggaaaggac gtgattgctg ttggaaagat caacgatatt
    17341 ttcaacggac aaggaatcac tgaatctgtc cgcacgaaaa gcaatatgga tggcgttgac
    17401 cagctgctga aagttatgaa aaacgatttt tcaggaatca gctttaccaa tttagtagat
    17461 tttgatgcat tatacggtca tcgtcgggat gtagtcggct atgcgcatgc gattgaagaa
    17521 tttgacttac gaattccaga attgcttgaa gcgatggaag aagacgactt gctaatgatt
    17581 acagccgatc acggaaacga tccgacgttt actggtaccg atcatacgcg agaatatgta
    17641 ccgttattgg tttacagtaa aaaaatgaaa gaacaagggg cattgcctca aggatattat
    17701 tcagatattt cagcaacgat tgctgagaac tttagtgttt cttcgactga aaatggcgaa
    17761 agcttcttga aattattaaa ataatctttt taaataggag aaagcaaatg accaagttaa
    17821 gcgagatgtt acaagaaacg acccagttta tccttgataa aggagtaaaa gaaattgatt
    17881 ttggtttgat tctaggttca ggattaggtg aattagcaga tgaaatcgaa gatgctgtaa
    17941 tgatttctta tgatgagatc ccgttctttc ctgtttcgac agtagttggg catgcaggaa
    18001 agctagttta tgggtctctg gcaggcaaga aagtgttagc catgcaaggg agattccact
    18061 actatgaagg tcattccatg caaacagtga cctatccagt acgtgtgatg gccggactag
    18121 gggcccattc agtcattgtg actaatgcat gtggtggtgt caacgaatca tttgtaccag
    18181 gagatttgat gctgattacc gatcatatca attttactgg tgaaaatcct ttgatcggtc
    18241 cgaatgaaga tgcaatgggc cctagatttc cagatatgag tcaagcttat acgcctgact
    18301 atcaagcaac agcaaaagca gcagcagaaa aactaggttt ggatcttaaa gaaggcgtat
    18361 atatgggcta ttctggacca acttatgaaa cgcctgcgga gatccgaatg tctcggacaa
    18421 taggagccga tgcagtaggg atgtcgactg ttccagaagt catcgtagca gctcacagcg
    18481 gattaaaagt gttaggaatc tcttgtatta ccaacttagc agcaggtatg caagcgaatc
    18541 tgagccatga agaagttgta gaaacaactc agagagtcaa acaatctttt aaagcattgg
    18601 ttaaagaaac attgacatta ttgtaatagg aggaacaata aaatgagtgt gcatattgaa
    18661 gcgaaagaag gcgagatcgc cgataagatt ttacttccgg gagatccttt gcgagctaaa
    18721 tatattgcgg agactttttt ggaagatcca gtttgttaca atcaggtacg cggtatgtta
    18781 ggctttaccg gcaaatataa aggagaacgt atttctgttc aaggaacagg aatgggaatg
    18841 ccatcagcca ccatctatgc ccatgaattg atccagtcgt atggtgtaaa gaaattgatt
    18901 cgagtaggta catgcggtgc attgtcaaaa gacgtccatg ttcgtgattt agtactagca
    18961 caaggtgcag cgaccagttc ttcgatgatc gaaaaaaatt tccaagcctt tcattttcct
    19021 ccaatcagtg atttcaatct attactgaaa gcatatgaga tagctaaaga aaagggctat
    19081 acggttcatg taggaaatgt tctttcggaa gattctttct acaaagatga tttgacagag
    19141 acgttccaat tagctgaact tggcgtttta ggagtggaaa tggaagcagc ggctctttat
    19201 tacttaggcg cgaaatacca tgtgcagaca ttaagtttga tgaccgttag cgaccatttg
    19261 atcaccggtg aagaaacaac agctgcagaa cgtcaatcta cgttcaatga aatgatcgaa
    19321 gtaggattgg aaacagcgat agcagaataa gaaggaaatg aaaaagctgc cctgcattaa
    19381 gtaataagaa catccaagca aaaatagctc ctcatgtttt tgacttggct ggacttatta
    19441 ttgcaaatgt aagcttttga acaccgttta ccaagaggtt gtgaatctgc cgtttaactc
    19501 tgagtcacaa ggaacaattt caaaaaacag cttttcatgt ttgatgaata ttgtgacttg
    19561 tgaccgagaa gttggcagga gaaaaccgtt tatacgaaaa aagggactgt gacaaatcgt
    19621 gtcacagtcc cttttttata gtgtttttta taggtagtat tttttagcaa ctgttaagtc
    19681 atcatttaat tcataaacaa gtggttgacc tgttggaatt tcaagatcca tgatgtcttc
    19741 gtcagagata ccttcgatat gtttagctag ggcgcgtaat gagttaccat gtgcagctac
    19801 taaaacagtt ttgttgtcta gtaatgctgg agcaatttca tcttgccaga atggcaaagc
    19861 gcgttctaat gtcactttca agttttctcc accaggaata tcgcgtttgt ctaacattgc
    19921 gtaacgacgg tcatttgctg cagaaccttc gtcagtttct tccattaatg gaggaagagt
    19981 atcgtaagaa cgacgccaga tgtgcacttg ttcgtcgccg tatttttcag ctgtttcttt
    20041 tttgttcaaa ccttgtaatt taccatagtg acgttcgttt aagcgccatg atttgatttg
    20101 tggtacccaa agttgatctg aatattctaa gatcaagtta catgttttga ttgcacgagt
    20161 taaaacagaa gtgtaagcaa catcaaattc gattccagct tctttgattt tgcgtccgcc
    20221 ttcttttgct tcttcgattc cttctggtgc tagatttaca tcagcccaac cagtaaattg
    20281 gttcaatgca ttccattcac taagaccgtg acgagaaaaa actaattttg gcatgtttag
    20341 ctctcctttt aagataggtt tatatttctg aaattttcag ttctgtttta ttgtacaatt
    20401 ttttggaaga gatttccatc gaaaaattag ataagattct atgagaaaac aagtagagaa
    20461 tataaaaaaa cgaacagtgc ccacgcaact gttcgttttt aggtattaga tgcataattg
    20521 ctcgatttct ttttcaggta aattgatata tttataacat gttccaactg aaacaccgca
    20581 ttttgaagaa ataaattgga tcgtttcttt tttttcataa tagagacgtc ggattttttt
    20641 gatcgttttt gcatcgattt ttggtcttcc gccgattttt ccttttttac gagcttcatc
    20701 taatccaact aatgtacgtt ctttgatcaa tgcgcactcc atgtttgcta ggttttccat
    20761 taattggaaa tagatttttc ccatttcgcg cgtatcgatt ccttcatcaa gactaatcaa
    20821 gtgcagcccg cgatctttga ataattgcgt gagttctgtt aattgccgcg tagatttgcc
    20881 taatcgatga agttggcaaa tgactagtgc atcgccttgt tgcatgtcag cgatcactga
    20941 ctccaaatga acagcagtag aatcattttg ttccacttca tatgtctcat ggaaaatttc
    21001 ttgacaacca aagtcagata attgatcaac ctgagttgtt aaatcaacgt catttatggt
    21061 tgtgcgtgca taaccaataa ttttcatggt gattcctttc tagcagtgat tttctaactg
    21121 gatataacag taaaaatgta gcataaagta cgtcaaaaag atagtttttt cgcaaatttt
    21181 ccgctgtctt tttttattgg atgatcaagc agtttttctt tatttacgac ggattgcgtc
    21241 aagaatctca acgccttttt ctgagccgac aactacttta gaaaccatgt ttaggaaaag
    21301 cccatgttct actaccccaa caagttcatc taaataggta gctaagcttt ctggctgttc
    21361 gatgacttct agatgcagat caatgatgta atgaccacca tctgtggtta agacttctcc
    21421 gttttcgtct gtacgcaaga cgggttggaa tcttttttct tcaaaaatat gaagtagctg
    21481 ttgggagcca taagggatca cttcaactgg gagtgggaac ttcccaagtt tatcgactaa
    21541 tttagaagaa tcaacgatcc aaatcgtttc ttttgagtac gtagcgacta ttttctcgaa
    21601 aagcaaagca gcgccgccgc ctttgatccc ttgaaaatca gcactgattt catcagcacc
    21661 gtctattgtc aaatcgacta cggggacttc atcaatactc tttaacggta ttcctaattt
    21721 ttccgcttgt tcttttgtcg catttgaagt agtaactcct gtgatacgta aaccttcttc
    21781 acgcatacgg cgaccaatct cttctaccat ataataggcg gttgaaccgg tcccgagacc
    21841 tacgatcatc ccgtccttta cgaatttcgc ggcttcaatg ccagctaatt gttttaaatt
    21901 catctttgcg tccacctttt tttagtttga gcatcgtgct ttcctcacta ttttagcgtg
    21961 atttacggta aaaagatacc attctttttt attccaataa agctttttca aaaaaaattg
    22021 catctcttgt tgacatgttt tttcattcgt gatattctaa caaaggtgct ttatctacag
    22081 gtttctgtga agaaacgtgc tgactgttta tcaaagcata tttgataagg atgcagacat
    22141 tgcatttatt ttttatcaca aaaaaagaac cacctggatg tgtggaacta gacgcaaatc
    22201 gtgggaagga ggaaacaagg aatgcctaca attaaccaat tagtacgtaa acctcgtaaa
    22261 tcaaaggtgg aaaaatctaa ttcacctgct ttgaacaaag gatataacag ctttaagaaa
    22321 actcaaacta acgtaaactc acctcaaaaa cgtggggtag caactcgtgt gggtacaatg
    22381 acacctaaaa aaccgaactc agctttacgt aaatatgcac gtgttcgttt gtcaaactta
    22441 attgaagtta ctgcttatat cccagggatt ggtcataact tacaagaaca cagtgttgta
    22501 ttattacgcg gtggtcgtgt aaaagactta ccaggggtac gttaccatat cgttcgcggt
    22561 gctttagata ctgctggtgt taacgaccgt aaacaaagcc gttctaaata cggaactaaa
    22621 aaacctaaag cttaatttta attattaaac aaatgaaact tgcttctact atatagaagt
    22681 aattactatg aacaatattt cggaaggagg agttacggat gccacgtaaa ggtcctgttg
    22741 caaaacgtga tgttttacca gatcctattt ataactcaaa attagtaact cgcttgatca
    22801 accgtgtaat ggttgacgga aaacgcggga ttgcagctaa cattatctat aatgcctttg
    22861 acttgatcaa agaatctaca ggtaacgatc cattagaagt gtttgaacaa gcaatgaaaa
    22921 acgttcaacc tgttcttgaa gttaaagcac gtcgtgttgg gggttcaaac taccaagtac
    22981 cagttgaagt tcgccctgaa cgccgcgtta ctctaggtct tcgttgggta gtaaactacg
    23041 ctcgcttgcg cggtgaacac acaatggaag aacgcttagc aaaagaaatc atggatgctg
    23101 caaacaacac aggtgcttct gttaaaaaac gtgaagacac tcataaaatg gctgatgcca
    23161 accgtgcgtt tgctcactat cgctggtaag attctctctg tttggtcggc ttttataagt
    23221 cgacctcaga tagtgtcctt atttttagca actagatagt aaagagagga gaaacaagga
    23281 gaatgaccag agaattttct ttacaaaaca ctcgtaatat cggtatcatg gcccacatcg
    23341 atgctggtaa aacgactgct actgaacgta tcttgtacta tactggtaaa atccataaaa
    23401 ttggtgaaac tcatgaagga gcttcacaaa tggactggat ggaacaagaa caagaacgtg
    23461 gtattacgat cacttctgct gctactacag cacaatggaa gggtcaccga atcaacatca
    23521 tcgacacacc aggacacgta gacttcacag ttgaagttga acgttcattg cgtgtacttg
    23581 atggtgctgt aactgttctt gatgcacaat caggtgttga acctcaaact gaaacagttt
    23641 ggcgtcaagc aacaacttat ggtgtacctc gtatcgtatt tgcaaacaaa atggacaaga
    23701 tcggtgctga cttcttatat tcagtatcaa ctttacatga ccgtttgcaa gcaaacgctc
    23761 acccaatcca attaccaatt ggtgcggaag acgacttcac aggaattatc gaccttgtaa
    23821 caatgaaggc tgaaatgtat acaaacgatc ttggaacaga gatcgaagaa acagaaattc
    23881 ctgaagaata ccgcgaatta gctgaagaat ggcgcgaaaa attggttgaa gctgtagctg
    23941 aaactgatga agaacttact ttgaaatact tagaaggtga agaaatcact gaagctgagt
    24001 taaaagaagg tatccgccgc gcaacagtta acgtagaatt ctacccagta ctatgtggtt
    24061 cagcatttaa gaacaaaggg gttcaattac tattggacgc tgttcttgat tacttgccat
    24121 caccgttaga tatcccagct atcaaaggga ttgaccctaa aactgatgaa gaagtagaac
    24181 gtcctgcaga cgacagcgct cctttctcag ctttagcatt taaagttatg actgacccat
    24241 tcgtaggtcg tttaactttc ttccgtgtgt actctggtgt tttacaatca ggttcatacg
    24301 tacaaaacgc aacaaaaggc aaacgtgaac gtgttggtcg tatcctacag atgcatgcca
    24361 actctcgttc agaaatctct gaagtttacg ctggagatat cgctgctgct gttggtttga
    24421 aagacacaac tactggggac acactatgtg atgaaaagag tctagttatt cttgaatcca
    24481 tggaattccc agaaccagtt atccaagtcg ctattgaacc taaatcaaaa gctgaccaag
    24541 ataaaatggg tgtggcttta caaaaactat ctgaagaaga tcctactttc cgtgctgaaa
    24601 caaacgtgga aacaggcgaa acaatcatcg ctggtatggg tgaacttcac ttagatatca
    24661 tcgttgaccg tatgcgtcgt gaattcaaag ttgaagctaa cgtaggtgcg cctcaagtat
    24721 cttaccgtga aactttccgt gctggcactc aagccgaagg taagtttgta cgtcagtctg
    24781 gtggtaaagg tcaatacggt cacgtatgga ttgaatttac accaaatgaa gaaggtgctg
    24841 gtttcgaatt tgaaaatgcg atcgtcggtg gggttgttcc tcgtgaatac attccagctg
    24901 ttgaaactgg tttgaaagat gctatggaaa atggtgtctt agctggctat ccattagtag
    24961 atatcaaagc taaactttac gatggttcat accatgatgt cgattcaaat gaaactgcct
    25021 tccgtgttgc tgcttctatg gcattacgtg cagcagctaa gaaagctaat ccagtaatcc
    25081 ttgaaccaat catggctgta gaagttgtta tccctgaaga ttatttaggt gatgttatgg
    25141 gtcacgtaac tgctcgtcgt ggacgtgttg aaggtatgga agctcgcgct ggcggccaac
    25201 aagttgttcg tgcgatggtt ccacttgctg aaatgtttgg ttatgctaca actcttcgtt
    25261 ctgctactca aggtcgtggt acatttacaa tgacatttga ccactacgaa gatgtaccaa
    25321 aatcagtaca agaagaaatc attaagaaaa acggcggaaa agctgaataa tttcttaaaa
    25381 ccggatagtc ctgcaaagag attatccaaa gagactatat ttttaatata gtttcgtgta
    25441 aaataattat cgatgatatg ggcatgtggt gtaccttgtg tacatcatat acctatcaga
    25501 caaataaaaa atatattgta tttttaggag gacattttaa aatggcaaaa gaaaaatttg
    25561 accgttctaa accacacgtt aacattggta ctatcggaca cgttgaccat ggtaaaacta
    25621 cattaacagc tgcaatcaca actgtactat ctaagaaaaa cggcggccaa gctatggctt
    25681 acgatcaaat cgatggtgct ccagaagaac gcgaacgcgg aatcacaatc tcaacagctc
    25741 acgttgagta tgaaacagat acacgccact acgctcacgt tgactgccca ggacacgcgg
    25801 attacgttaa aaacatgatc actggtgctg ctcaaatgga cggagctatc ttggtagttt
    25861 ctgctgctga cggcccaatg cctcaaactc gtgaacacat cctattgtct cgtcaagttg
    25921 gtgttcctta catcgttgta ttcttgaaca aagtagacat ggttgatgac gaagaattac
    25981 tagaattagt tgaaatggaa gttcgtgacc tattaacaga atacgaattc cctggtgacg
    26041 atgttcctgt agttgctgga tcagctttga aagctctaga aggcgacgct tcatacgaag
    26101 aaaaaattct tgaattaatg gctgcagttg acgaatacat cccaactcca gaacgtgaca
    26161 acgacaaacc attcatgatg ccagttgaag acgtgttctc aattactgga cgtggtactg
    26221 ttgctacagg tcgtgttgaa cgtggacaag ttcgcgttgg tgacgaagtt gaagttgttg
    26281 gtattgctga agaaacttca aaaacaacag ttactggtgt tgaaatgttc cgtaaattgt
    26341 tagactacgc tgaagctgga gacaacattg gtgctttact acgtggtgtt gcacgtgaag
    26401 acatccaacg tggacaagtt ttagctaaac caggtacaat cacacctcat acaaaattct
    26461 ctgcagaagt atacgtgttg acaaaagaag aaggtggacg tcatactcca ttcttcacta
    26521 actaccgtcc acaattctac ttccgtacaa ctgacgtaac aggtgttgtt gaattaccag
    26581 aaggaactga aatggttatg cctggcgaca acgtaacaat ggaagttgaa ttaatccacc
    26641 caatcgctat cgaaaacggt actaaattct caatccgtga aggcggacgt acagttggtg
    26701 ccggtgtcgt tacagaaatc aaagcttaat tcacttaagt tttaaagtaa agcaacattt
    26761 attcggacgt aatcgctcgg aacgaaggtc ttaggatctt tgttccgagc ttt
//
LOCUS       DANKEF010000034        26764 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000034 DANKEF010000000
VERSION     DANKEF010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26764
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.034"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(23..1954)
                     /locus_tag="PWZ48_001666"
     CDS             complement(23..1954)
                     /locus_tag="PWZ48_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2261324.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(1972..2886)
                     /gene="pfkB"
                     /locus_tag="PWZ48_001667"
     CDS             complement(1972..2886)
                     /gene="pfkB"
                     /locus_tag="PWZ48_001667"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2261325.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(2883..3635)
                     /locus_tag="PWZ48_001668"
     CDS             complement(2883..3635)
                     /locus_tag="PWZ48_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2261326.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(3795..4004)
                     /locus_tag="PWZ48_001669"
     CDS             complement(3795..4004)
                     /locus_tag="PWZ48_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261327.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(4015..5342)
                     /locus_tag="PWZ48_001670"
                     /pseudo
     CDS             complement(4015..5342)
                     /locus_tag="PWZ48_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5478..5633
                     /locus_tag="PWZ48_001671"
     CDS             5478..5633
                     /locus_tag="PWZ48_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261328.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(5707..6294)
                     /locus_tag="PWZ48_001672"
     CDS             complement(5707..6294)
                     /locus_tag="PWZ48_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDL2261329.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(6377..7627)
                     /gene="clpX"
                     /locus_tag="PWZ48_001673"
     CDS             complement(6377..7627)
                     /gene="clpX"
                     /locus_tag="PWZ48_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HDL2261330.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(7874..9163)
                     /locus_tag="PWZ48_001674"
     CDS             complement(7874..9163)
                     /locus_tag="PWZ48_001674"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDL2261331.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(9317..10249)
                     /locus_tag="PWZ48_001675"
     CDS             complement(9317..10249)
                     /locus_tag="PWZ48_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261332.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(10406..11065)
                     /locus_tag="PWZ48_001676"
     CDS             complement(10406..11065)
                     /locus_tag="PWZ48_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HDL2261333.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(11068..12093)
                     /gene="sppA"
                     /locus_tag="PWZ48_001677"
     CDS             complement(11068..12093)
                     /gene="sppA"
                     /locus_tag="PWZ48_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDL2261334.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(12106..12396)
                     /locus_tag="PWZ48_001678"
     CDS             complement(12106..12396)
                     /locus_tag="PWZ48_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261335.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            12805..14610
                     /gene="glmS"
                     /locus_tag="PWZ48_001679"
     CDS             12805..14610
                     /gene="glmS"
                     /locus_tag="PWZ48_001679"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDL2261336.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(14682..16037)
                     /locus_tag="PWZ48_001680"
     CDS             complement(14682..16037)
                     /locus_tag="PWZ48_001680"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDL2261337.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(16061..17230)
                     /locus_tag="PWZ48_001681"
     CDS             complement(16061..17230)
                     /locus_tag="PWZ48_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261338.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(17227..18114)
                     /locus_tag="PWZ48_001682"
     CDS             complement(17227..18114)
                     /locus_tag="PWZ48_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HDL2261339.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(18303..21995)
                     /gene="nifJ"
                     /locus_tag="PWZ48_001683"
     CDS             complement(18303..21995)
                     /gene="nifJ"
                     /locus_tag="PWZ48_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDL2261340.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            22194..22715
                     /locus_tag="PWZ48_001684"
     CDS             22194..22715
                     /locus_tag="PWZ48_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HDL2261341.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(23414..23971)
                     /locus_tag="PWZ48_001685"
     CDS             complement(23414..23971)
                     /locus_tag="PWZ48_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261342.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(24150..24761)
                     /locus_tag="PWZ48_001686"
     CDS             complement(24150..24761)
                     /locus_tag="PWZ48_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261343.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(24874..25131)
                     /locus_tag="PWZ48_001687"
     CDS             complement(24874..25131)
                     /locus_tag="PWZ48_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261344.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(25728..26006)
                     /locus_tag="PWZ48_001688"
     CDS             complement(25728..26006)
                     /locus_tag="PWZ48_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261345.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(26040..26291)
                     /locus_tag="PWZ48_001689"
     CDS             complement(26040..26291)
                     /locus_tag="PWZ48_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261346.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT         7555 a         5767 c         4584 g         8858 t
ORIGIN      
        1 cccagcctct tcttttttat ttctatgctt ctgctactgc aggagctttt ctcaataaac
       61 cataaatcac accagatacg actgctccga tagcaataaa caccaaatac atgaccgggt
      121 gactcagtaa gaatacaacg aaaatcccgc cgtgaggagc cagtaatttg attccgaaag
      181 ctccgactaa tccgccagtt aaagcagaac caataataaa acttggaatg gcacgaatcg
      241 ggtcagctgc tgcaaatgga atcgatcctt ccgtgacaaa cgatagtccc atcacgatgt
      301 tcgtcaaacc agcatcttga tctgttttgc tgaatttatt cttaaacaaa cgtgtagcaa
      361 cgaaaattgc caatggaggt accattcctc cagccataac agaagccatc acgacacttc
      421 cgccagttgc aacagtcgta gctaaagttg ctgtaccaaa cacataagcg gctttgttga
      481 ttggaccgcc taaatctgcc gccatcattc cagctaataa tgcaccaagt aaagcagcat
      541 tcgaaccact catacttgtc aagaaaccgt tcaatgcatc attgattgct ttcataggga
      601 cattgaccag caacatcaag aaaccagtaa tcagtaagcc aaacactgga taaaagagga
      661 tcgttttaat tccatccaat gattttggta agttcttgaa taatttacgt aaaaatacga
      721 tgacataacc agctaagaat ccaccaatca gtgcacctaa gaatcctgca ccgcctgtat
      781 tagctaatgc accagcagca aatccaacga tcaaaccagg gcggtcaccg atacttgaag
      841 caataaatcc tgctaatacc ggcagcatga aaccaaatgc agcttgtccg atttggttga
      901 accagctagc agcttgattg taattcccta gatttgataa ttgatcatgt ggcacgccaa
      961 taaattggtc gatcatgaaa gaaagcgcaa tagcgattcc tccaccaata acgaaaggta
     1021 acatgtgtga tacgccgttc atcaaatctt tgtaaattct ttgtccgata gtaccatccg
     1081 catttccttc ttctgaactg ctttcttttc catcaccatg gaatactggt gcagtaccac
     1141 taagggctag attgatcaac tcttctggtt tacggatacc atcactgaca ggacgattga
     1201 tcagttcttt tccgtcaaag cgattcatct ctactttttt gtctgctgca acgattacac
     1261 cttctgcacg ggcgatgtct tcagcagtca atcggttttt gattccttct gaaccgttcg
     1321 tttcgacttt gatctccaca cccatttctt tcgctttttt cttcaaagca tcttctgcca
     1381 tgtaagtgtg agcgatccct gttggacaag ctgtaacggc aacaacgaat tttctttctg
     1441 gatgagcaga agcttctgca gcttgtgttt cttgttctgc tttttcttct gcttcttttt
     1501 gctcttcagc tgtttggaac aagtcttgga cttcttctgg tgtatttgct tcttttaatt
     1561 ttcctacgaa atctgcatca atcagcaatc gagatagtgc tgctaatgct tgcaaatgtg
     1621 tgtcgtttgc gccttctgga gctgcgatca tgaaaaacag ataagttggc tgtccgtcta
     1681 aggcttcata atctactcct ttttggcttt ttgcgaataa aactgttgct tctttaactg
     1741 cactgttttt cgcgtgaggc atggcgattc catcaccaag acctgtagaa gtttgtgctt
     1801 cgcgggctag aattccttct ttatatgttt cgatatcgga aatccggccg gcatcaaaca
     1861 ttttttggac catttcatcg attgcgcctt ttttatcagt cgcttgcaaa tccatgatca
     1921 tcgcttcttt gaccattaaa tccttgatat tcatgattat cctccttgaa actaatcaat
     1981 ttttgtgatt tcaacttctg gcagcaactc atcaatcaat tcgcgagctg ccaagtcatc
     2041 agaaaaagct gttgcgcttc cgcaagcaac tccccattta aaggcttcga ttggatcttt
     2101 cgtacggctg taagccccaa taaagccagc aatcattgaa tcccctgcac ctactgaatt
     2161 tttcaatggg cgaactaata cattcgtttt ataagttcct tgttccgtga agagcaaagc
     2221 accgtctcca gccattgaga tcagcacatt ctgtgcccct tcagctagta attttttccc
     2281 atacggaatg atttcttcta cagattggaa ggttgtgttg taaagctcag ctaattcgtg
     2341 attgtttggt ttgatcaaca acggtttctt aacaagtgca ttttttaggt catcacctgt
     2401 tgtatcaatc acgaaatctg cacctttttc tcgaatgata tcaatcagtt cttgataaaa
     2461 tccttttcgc aggctagctg gcgcgcttcc tgacaatacg acgatatctc cttgtcctac
     2521 tgttcctaat gcttgcttca actcatcgat ttcttcatct gaaatagatg ggcctaagcc
     2581 attgatctca gtttcagtgt ctgattttag tttgatattg attcttgtat cttcttttac
     2641 ggttgtgaac aaagactgga tttgttcttt ttcaagccac ttcgaaataa attcgccagt
     2701 gaaccctcct aaaaacccta gtgctgtaga ttgagaatcg atccgtttca aaatacggga
     2761 aacattgatc ccttttcctc caggtaactt gaagtcttcc ttcattcggt tcaaatcgcc
     2821 tagttttaac ccatctacgc gtacgatata atcaattgat ggattaagtg ttactgtgta
     2881 aatcattgta ttgcctcctt aattgttgtc ttttcacgga attcattaac gaattctaat
     2941 ggacaacgct cggttatgat cgtggctgct tccaaaggtg caacagaggt gaaggacaac
     3001 tggttgaatt tggtatgatc aatcaataca aatgtctctt cggaattttt catggctaaa
     3061 cgtttaacag ctgcctcttc tggatccgga gtcgtgaatc cttgttcaag atgggcaccg
     3121 ttcattccca taaatgcttt attaaaatga aaatttttta gttgttctat cgcattagat
     3181 cctaaaacag cattagttgc cagctttatc gttccaccta aaataatggt agcaagctcc
     3241 aaatcaacta aatttgccgc atgttgaacg gaatttgtca ctactgtgat ctgttttcct
     3301 tgcagaaaag gaatcatttc taacgtagta gagccagcgt ctaaataaat gacatcttcg
     3361 tcattaatgg tttcagctgc cgatttggcg atttgctgtt tttcgtgaac gtttttgagt
     3421 gatttttcac tcatattctg ctcaaaattc aataattgct ctttcttagc tccgccatga
     3481 actctttcta gaaacccact attctccagc tcttgtaaat cacgtcttat cgttgattca
     3541 gaagcattca aaaggtttac aagatcttgt gatttcacga ttttctgctg ttcaagcaag
     3601 tctaggattt tttgtctgcg ttcctctgta agcattttca cgatcacctc aatacaaata
     3661 atagcatatt ttttttctac tagcaatata aatcagtcaa aaacgttcat aaaaattata
     3721 aatcagtcaa aaaaaaaata aaaaaccagt cagaaagact ggttttttat ttttttgtct
     3781 atcatcaagc cgttttatcg ttttttattt tcgcttcttg tttttttcat ataaacagaa
     3841 gcaggttttc tttccttatg aagaagataa taataaagtc ccatcccgcc aaacatgacc
     3901 aaaaaaccga tgactgatcg aataggaaaa ttaggaacaa aaaagagaca gacggataat
     3961 aatgatgccg gaaccattat tcgataatag agttgtttat ccattttctt cacctcatat
     4021 tttttgtaaa gtaatcaatt gttctaattc tcggtaatct aaaaattgca cacgcagatg
     4081 ctgctcacct ttcttgacat cgaattggat acttcgccgc tttttcttta ttcgtttgaa
     4141 ggtaacatca gagagtctat gtttgatttc ctttaacggt aattctgaca gggattctga
     4201 aggaacatag attcttaagg ataaaagata ctcttgatat ttttcatctg ttacctctac
     4261 ttctagatat tccctaatat aataacaata agcttcgatt ccattatcca aagcaacagt
     4321 cggatcgata actttatagc acatcatgcg tgatcgattc tcgtcaaccc aaggaaaata
     4381 cgcctgagca tttagtgttc cgcgaacacg cctttcttta taatctcctt gttggatatc
     4441 ataacttttt gttacaaaaa atgaagcatt ctttttagga acaggttgca tttgtgcatt
     4501 ctgatactgg acagattgga tcgtatggtc agtgtaaatg tattcaacga atggcggttt
     4561 caataaaccg acaccattgt cccaagcaag atgatcaaac ccaagctggt ccctaaaata
     4621 ggattgaaac atttgattgt ttggcttttg ccagttaaag gggtctcgat aaagctggat
     4681 acatatgact tcgttttcta attccttcac tttttctact tgttggatgt cgagaaaaat
     4741 cggtttaggg atgatttgat gataatagcg actataatac atttcccaac acgaagtgaa
     4801 acataaacct tggtaaaaaa gatcgtatcc tggaaattgg ttgcaatcga ctactacttc
     4861 cccgttttgt tgtttcattt taggtaaatg ttcgatttca tcctttttct caaacaatag
     4921 ccgttgagag atctgttttg tattgtgata gataaactcg ctataagctc gcatataggc
     4981 gaacacccca tgagcaatca tttttttctg aatgaagtct cgaatcaaaa attgcttttc
     5041 tttgaaaaca gagatagaca atacattttt tatcaataat tgattatctt ccaagcgaaa
     5101 actacattcg ttctcgtcat ttcctacaac taaccaactt tcgtctctta ccgatgctag
     5161 cattattttt tcttgaagct ttcgaagatc atacggatcc gcttgttgtg tgaaagagcg
     5221 gataaatgtt ggctggatca acagacgtgt caaaatagcc caatcgctta tttcctgttt
     5281 ttttcttctt catttaagtc tgtgagctgg atagtcatca ataatttatc ttcgccaatc
     5341 attttctcac ctctttcaaa tctttttata atttaattat aaccaatgat aaagaaaaac
     5401 taaaacttta tgtcttagaa accaatttgg acgattacat gatagactaa gtaaaaagaa
     5461 acaaagaggt gtttacaatg gggttgaaat ttgaacgtac agataattta gataaacttt
     5521 tcgaatcatt tgctgtcgat ccggaaaaaa cagttgcacc aaaagaagag atcgatcgat
     5581 ttctaaaacc acagccacaa aatgaaaaga aaaaagacaa gaaacaaaag taagttaagc
     5641 tcaaaaaaag acttaccagc caaactttta tgaaagagtt tgactggtaa gtctttttta
     5701 ttctttttat gattcaattg cttgttcgat tgctgcccac gcttcttctt ttccctgttt
     5761 tgtaacggaa gaaaagagaa taaacgtgtc attgcgatca aaatccaacg cttttttgat
     5821 ttcgctctca tgcttgttcc atttaccacg agggatctta tctgccttag tggcaacaac
     5881 aattacaggg atatcataat acttcaaaaa ttgatacatc tggatatctt cttgagaagg
     5941 cttatgacgc aaatctacta aagaaacgac cgcacgtagc tggtctctag tcgttaaata
     6001 tgtctcgatc atctttcccc attttgctcg ctcagttttg gatacttttg catacccata
     6061 tcccggaaca tcgacaaaat gtaatgcgtt ctcgatcaaa taaaaattca atgtctgggt
     6121 ctttcctggc ttaccggaag tacgggctaa attctttcga tcaatcaatg tgttgataaa
     6181 agaagatttc cctacatttg atcgtcctgc cagagctatt tctggcaatt gtgtatctgg
     6241 atattgtttt ggtgccactg cacttatgat aatttcagct tgatggactt tcatggtctc
     6301 tctccttaac aaggtgatga ataaagcaag cggctgtcgc aaaagccaaa atgactttta
     6361 cggcagcctc ttttcatcaa cccgcttttt tctctgaatc tttatagatt actgttggct
     6421 caccagacaa ttcagctgct tcttttgtga taatcacttt ttcgatgttt tcatttgaag
     6481 ggatatcaaa catcacatcc atcatgattt cttcaatgat ggaacgaagt cctcgagcac
     6541 cggtattgcg ttcgattgct tttttcgcaa tggctctcaa cgcttcaggt tcgaattcaa
     6601 gttcggtatt atctagagaa agaagttttt gatattgttt gactagcgca tttttcggct
     6661 cagtcaagat ccgtaccaga tcatctgttg tcaatttctc taaagctgcc ataacaggta
     6721 atcgtccgat aaactcaggg atcaatccga attttagtaa atcttcagga atgatatgct
     6781 gcatgacact ttcgtcttca gaaagttttt ggttttgtgt accaaatcca atcgtctttt
     6841 cgcccatacg attcttcaca atcgtttcga tgccatcaaa tgcaccacct acgatgaaca
     6901 atatgttcgt cgtatcaatc tgaataaatt cttgatgtgg atgtttacgt ccgccctgag
     6961 gtggcacact tgcaacagtt ccttctagaa ttttcagcaa ggcttgttgg acaccttcgc
     7021 cggaaacgtc acgagtaata gatacatttt cgcttttacg cgcaatttta tcgatctcat
     7081 caatatagat gatccctttt tctgcaagtt ccacattgta atcagcagat tgaagcaatt
     7141 taagcaaaat gttttctaca tcttccccca cataaccagc ttctgtcaaa cttgtcgcat
     7201 ctgcgattgc aaaaggtaca ttcaatgttt ttgctaatgt ttgtgctaaa aatgttttac
     7261 cagaccctgt aggtccgatc aagcagatat tgcttttttg cagttcaaca tcatctgtgt
     7321 tttcttgtgc ttgctgattg acacgtttat agtgattgta tacggcaaca gctaacgagc
     7381 gttttgcgcg atcttgtcca atcacgtagt tattcaatac ttcaagaatt tctagtggtt
     7441 tagggacttc agtgaattcg cgtacagctt cttcataaaa ttcttcatca atgatttctt
     7501 tacataggtc gatacattca ttacagatgt agacccctgg accagctacg atctttttca
     7561 cttcttcttg ggttttcccg caaaaagaac agcgtacggt ctcattgcta cttggattgt
     7621 catacatggt cttcacccct taacttgcaa aaagtatgca agaaactatc aaagacttta
     7681 cagtctttta ctatcataac atagtttagt aaaaagaaaa agtgtttaac aaaggatcaa
     7741 ctgacctata cctttgcatt ttttactctt aacaaaaaat aaggaactgt gacaaaatcg
     7801 tgtcacagtc ccttatttcg aataaacaat tttctagaag tagcttcttc agaaatgatt
     7861 tattacttga tttctattct ttcgcttctg tcgctgtacc tgtgatcaat tcgattgctt
     7921 ttttcattgt cacatcatgt tccagcatat cttctgtcaa cacgcgtttg atttgttcga
     7981 ttggcatgtt gtatgtttct gataattctt ggatttctgc attgatttct tcttctgttg
     8041 cttgaagatt ttcagcagct gcaattgctt cgatcacaag atttgttctt gtgcgcattt
     8101 ctgcttcacc ttcgaattgt ttatgcaagt cttcttctgt agaaccagtt aattgatagt
     8161 acatttcagg agagatacct tgacgttgca tgttgttcaa gaattcgtcc attgaacgat
     8221 gtacttcatc atgaaccatc acgtgtggca attcaacgat ttctgcgttt tcaacagcca
     8281 tacggatcgc tgcttcatct tttgcatcgt ccgctgcttt ttcttttgtt tctactaatt
     8341 ctttgcgata tttttctttc aattcgtcta atgttgatac ttcgtcatca acatcttttg
     8401 cgaattcgtc atctaattca ggcacttctt ttgcttttac ttcatggatt gtcactttga
     8461 agactgcttc ttttccagca agatcttctg cttgataatc ttctgggaaa gttacattta
     8521 catctagact ttcacctgat ttatgaccaa ctaattgatc ttcaaaacct ggaatgaatg
     8581 agcctgaacc taattcaagt gagtaatttt cgccttttcc gccatcaaac gcttcgccat
     8641 ctacaaagcc ttcgaagtcg attactactg tatcgccatt ttcagctgca gcatcttctt
     8701 tgataactaa ttcagcttgt gcttcttgtt cacgtttgat acgtgcatcc acgtcagcat
     8761 ctgttacttc acggtcttgt ttttcaacaa ctagatcttt atattgtcct aattttactt
     8821 ctggtttcac tgttacttca gcagtgatta cccagtcttg acctttttcc atgctttcaa
     8881 catcgatttt tggttgagaa acaggatcga tgccagcttc tttgacagca gcttcataag
     8941 cttctggtaa tacagcattc aatgcatctt catataaagc ttcttcaccg tacatacggt
     9001 tgaatacttg acgagaaact ttccctttac ggaatccagg tacgttcaag ctttttttca
     9061 ctttattaaa cgcagttgtt aatccttttt ggatttcgtc ttgcgcaatc gtaaatttta
     9121 aaacaccatc attcgtgcct tttttttcaa atgttgcagt catttaattc cctccgagtt
     9181 tagaaattca ttttatacat caaaaacgtt tcatgcatac ctttaaatag tacactaacc
     9241 ctttcaaatt gtaaacatat tgaatggata gaatccttgt ttttcataac atttttatct
     9301 ttttcactga taaaatctaa tggaagaaga tcatttgctg caaaattttt tctctttttt
     9361 ttaaaaaatc ttgtttttca tcttctgtac cttcatacat cgtttttcct gtacacgcct
     9421 ctacagtcag ttgaaaccag cgcttcgggt cttcgatgta tgtatcatag acaggataaa
     9481 caattgcaga ttgcaacgtg aattcttctt ttaacacgga actaagcagc gcatcttcat
     9541 tcccgtaacg atctgaaaat acacataatt gctggtaact tgcctgctgc tgcggctttg
     9601 gcagatcttt tggtatgact ttgactaact tttcttcaat cgtcaaatag aaaacaggat
     9661 ggcattcgtt caattgccta agtgtctcca gtaattttgc ccttgctaaa atatggacct
     9721 ctggcgatac aataagttta ctcgctgttt ggataaactc atgataagga agctgttcga
     9781 tctcatcaat agacgcaagc tgacgtgcag gttccaacga aggaagttcc tccgatttat
     9841 tgtggagtgc cttgatcttc tgcctctcca tttgtccgga taattcttct gcaaattgga
     9901 ttttttcaat gacccgctgc tcgaaagctt cataaaaatc tgtacttgct atcaattttc
     9961 tagcatataa aaaatcttgt gtactgatta acaagtcaaa ataaaattca gcaaactcct
    10021 cattttgaag atactgtttt tcatgcaaga gtgcttgctt caatgcttct tttttctcgt
    10081 ccaactcgta aagacaaaaa acaagcaggc tattagcctc aaagctttct ttcaactcgt
    10141 aagctcgttg aaataattct tttgcctgct gatattgctg gtttgctagt gcttgttgtg
    10201 ccactcgcat taaattctca tatttttctg gaaaatcaat cggcttcacg cagtgtcctc
    10261 ctcttgctca tattcagctc atgttcaata gtagcaaaac aaaaaaagca tgacaattaa
    10321 aaataactga agcaccctct aaaagctaga ttttagatct gacttttaga aggtgctttc
    10381 aactgttact gctttttatt gctcatcagc tgttgacttc ctggttgaac gctttgatca
    10441 gattgatcgt aacaacactt gtattagtga aaaaatcgcc cagatgttgc tttttctgat
    10501 taaagactgc tggaagatac cctaacatca gcaaaggaaa ttttagaatg aaccggcaag
    10561 caccttctct aactaaaacg gttgaccagc taagttcttc ttcatcaaag ctgatgactc
    10621 gaataccaaa gatcattttc ccaatcgttt ggccatgatt gaatttcgtc aataaaataa
    10681 aatacgctag ataaataagt aaggctgtga atccgtagag actcagataa gaatctgaaa
    10741 aattaagcca acctagattt ttggcaatac caagtgttaa acgcgtaata gctccgatac
    10801 acaaaagatc gaccaagaag gcaaacattc ggataaaaaa acctgcatag aaatacttag
    10861 gaaaatcatt tgcttttggc ggttcatttc ttgtgtccgt atactttttc catttacgtt
    10921 gtttcttttt gatttcttcg ggcgtcaaag gctctctttc aaaagagctc agtacctttt
    10981 gggaaaagtt ggagtcaggt cctacagcag gctgctgttg cttatctttt tcttgttcct
    11041 tattttcatt tgaaagctct gacatgctta ctcacctccg taataataca ttgcttttgg
    11101 tgattccgct gtccctagat tctcaagaac ggataagatc tgactagtct cagaaggctt
    11161 caatccctgg aattctgcta gtttacttcc taaccatgta ctagcaaacc cagatggcgt
    11221 ggaagaatat tctactagtt ccgcatcttc aagctgttgt tcctttcgta tagcttttaa
    11281 agcatcttca ggaaatccca attcatcgac taaacctact tcttttgctt ggacaccatc
    11341 ataaatcctt ccatcagcaa tttttttgac ttcttcttca gatttattcc gtccctcgct
    11401 gacgattgct acaaaacgac tatatgcgct gtcgatatat gcttgaagca ccgcttcttc
    11461 ttcttttgtc tctggtcgcg ttcctgaacc catatctttc aacgctccgc ttttaactgt
    11521 tgtatcttcc acacccagtt tttctagaag tcctgaataa ttcaatcctg acatgatcac
    11581 accaattgag ccggtcactg tttcttctgt tgcaaagatt ttatctgctt gagcagaaat
    11641 atagtagcca ccgctagctg ccatgttttt catgctgaca tacattggga tatcatgttc
    11701 tttacgaatc gtatccaatt tcttagcgat ttccgcactt tcgtagacac cgccaccagg
    11761 agaatttact tctaaaaaga tacctttgac tgttggatct tcttcaatcg ccttcagttg
    11821 ttccataaaa tttttatgat tgtaaccttc agaagcaaat aaacctgaag accctcccgc
    11881 agcaatcgtt ccatctactg ataattttac gattttttgc gtactgtctc cctcttccaa
    11941 cacatttgga gctagttctt catccccata taaccaactg ttcaatccct caatttgatc
    12001 gttttcctga ggcttagtca aactagctga aactaacgaa accacaaata atcctgcagc
    12061 aatcagaact gctgcccaac gtcttttatt cattctttct cctttctaat acaagttttt
    12121 tatgacgatc acagtttctg actgctcaga agtgtcgaac cgctctgctt tttcttcccg
    12181 cgcatacccg gaaaacgact cttttgttaa ttcatcttct accgtaaact cacgagcttg
    12241 agcaacttcc aaggcatcag gtgcgatttc ttggatgcta tcccagccag tatctaaaaa
    12301 tattttcgta tcatctgata caccagctga tgcttcgaat ttggacaatt gtttttttaa
    12361 cattcccagt gtcatgatct caatttttga atccattttt actcctcctc tatccgatta
    12421 ctttctattg taccgaactc ctgaaaaaga tgatagataa aaacccatta ttttttgaca
    12481 taaaaaggac atttgacaaa gaatttgata tataccaatt tattttttat tctttttttg
    12541 ttgacaagaa cattcgcaaa aggttatgat tttaaatgtt tccagataaa aaacaaaaag
    12601 gaggatagtt atacctttta ggatagcgcc aggtctgtgt aaacagatga cgaggagagg
    12661 gtttatcgaa agattcggcg gatggcccta gggactgcac tctacagaaa atccacaaaa
    12721 aatgtctgca aagataaaac aaaaggattt tcctgcagct ggaagcattc atacgaattc
    12781 attatagaaa gtaggatgtt ttttatgtgc ggaattgttg gaatgattgg attaaaaaat
    12841 gtaacaccag gattgattga cggtctagaa aaattagaat accgtggtta tgattcagct
    12901 ggtatctttg tctcagatgg cacaactgat tatctggtca aagcacaagg cagaattcaa
    12961 aatttgaaaa acaaaataaa tattgatact acaggaaata taggtattgg tcatacacga
    13021 tgggcaactc acgggcaacc ttctgaagaa aacgcccatc cacatacttc ccaaagtgga
    13081 cgcttcgtgc tcgttcacaa cggcgtaatc gaaaactttg aagaacttaa aattgcttat
    13141 ttagcaaatg accactttat tggtgagacc gatactgaga ttatcgctca tctaattgaa
    13201 actttcgcga aggatactac aacgcaagag gcgtttttaa aagctttgcg ggtcatcaaa
    13261 ggttcttacg ctttcgcttt gattgatcgg acagcacctg atgttattta cgtcgctaaa
    13321 aataaaagtc cactattaat cggactcggc gatggattca atgtgattgc cagcgatgca
    13381 atggctatgc ttgctcatac gaaggaattc gttgagattg aagatgaaga gatggttacc
    13441 gtcacatcag aaaaagttat tattcaaaat tttgcaggtg acatcgtaga acgttcttca
    13501 ttcgaagcac aagtagatgc ttcagatatc gagaaaggta cctatccttt ttatatgctg
    13561 aaagaaatcg acgaacagcc tattatcatg cgtcgtatcg ctcaaaaata cgtaacagaa
    13621 gacaatcgtg tctctcttga tcaaaagctg gtcgatacat tgtctgatag cgatcgaatc
    13681 tatatcgttg cttgcggaac tagttaccat gcggggttag caggtaaaca gactttggaa
    13741 aaattgacac agattcctgt tgaggttcat ttagccagtg aatttggcta taacacacct
    13801 cttctttcaa aaaaaccatt ctttattttc ttgagtcaaa gtggcgaaac agcagacagc
    13861 cgccaagtgc tggtaaaaat caaccgtctc ggctaccctt cacttacgat tacaaatgtt
    13921 gctggttcta ctctttcgcg ggaagcatcc tttacactgc tattgcatgc tggacccgaa
    13981 atcgcagtag catcaacgaa agcttatact gcacaaatag ctgtactaac tttattagcc
    14041 aaagctatcg gtgataaaaa agaatttgaa acttctcttg tattcgatat cactcatgaa
    14101 ttgagtttag ttgctaatgc gatggaatcg gtcatcgccc aaaaagatta tttagaagag
    14161 ttggcagaag tatatctcag cgatacaaga aatgcctttt acattggacg tggtgctgac
    14221 tacaacgttt ctcttgaagc tgctttgaaa ttgaaagaaa tcagttatat ccaggcagaa
    14281 ggttttgccg ctggcgaatt gaaacatgga acgattgctt tgatcgaaga aggaacacca
    14341 gtgatcggaa tcatcagtga agaagtgact ggtgcgcata ctcgaggaaa cctgaaagaa
    14401 gtcgaaagcc gtggcgctaa aacactagtt attgtctcag agggattaga aaaggaaagt
    14461 gaccagctgg tgctaccaac tgtccaccct tatctaacta ctctaaccac agtcgtaccg
    14521 actcaattac ttgcatatta cgcaacgcta ttacggggat tcgacgttga caaaccacgt
    14581 aatttagcga aatcagtgac agtagaataa taaaagaaca aggaccagtg tcagacatct
    14641 gacactggtc cttgttcttt tattttttat gaaaatatct cttaatccag tccgatttct
    14701 tctttgacta ctgccgcgat tttatcgaca tagtaattaa ctttctcatc agtaggcgct
    14761 tctgccatca ctcgaagtaa tggttctgta ccggaaggac gaactaggat tcttccttct
    14821 gatcccattt cagcttctgc ctcttcaatc acttttttga ttgccggaac atccatcgca
    14881 ccgtttttat cacttacgcg gatattcact aatttttgcg gatagattgt tacctctgct
    14941 gcaagctctg ataatttctt acctgtctgt ttcatgatat tcagtaactg aattcctgaa
    15001 agcatcccat ctccagttgt gttaaaatct aagaaaatca tgtgcccaga ctgctcgccg
    15061 ccaaagttat aatcgttttt gcgcatttct tctacaacgt aacgatcacc aacttgtgta
    15121 acgacatctt tcaaaccgat tccttccacc gctttgtgga atcctaagtt actcatgaca
    15181 gtcgtaacga tcgtatcttt tttcaatcga tttttttctg ccaagtattt agcacagata
    15241 aacatgattt tatcaccgtc gacaatgttt ccaagttcat ccaccgcaat aatacgatcg
    15301 ccatcgccat cgaaagctag tccagcatct gctccttttt caacgaccat ctcagctaaa
    15361 cgttctggat gggtagagcc tacgccgtca ttgatattta acccgtctgg atttgtgccc
    15421 attgtataaa aatctgtttc aagatcggca aacaaacgat taacacttgt tgaagttgct
    15481 ccgtttgctg catcgacaca aatggtcaaa ccggaaaggt caccgttgat cgtttgtaca
    15541 aggaattgag aatatttcaa taagccttct ggaaattctt ctacagttcc caatccttca
    15601 gctgatggac gaggcaattc atccacttca gcatctaata atgcttcaat ttctagctct
    15661 tggtcatcta ctagtttaaa accatctgca ccgaaaaatt tgattccatt atcttgtgct
    15721 ggattatgag aagcagaaat catgacccct gcgcttgctt tttgcaaacg tgtcagataa
    15781 gcaacacctg gtgtagagat cacacctagc tggaacactt cgatacccac cgataaaagt
    15841 cctgaaatca gcgcttcttc taacatttga cctgagattc ttgtatcccg ccctactaac
    15901 acgcgtgggc gttttccttc ttcttcatgc tggcttagga cataacctcc gcaccgtccc
    15961 agcttaaatg caagttctgg cgtaagttcc ttgttggctt caccgcgtac gccgtctgtt
    16021 ccaaaatatt tacccatagt tgtaaaatta ctcctttaac ttaattttct gaggaatttg
    16081 ttctgctcat ttgtgttgat tcgcttgttt ctgatgtttc ttctgtactt gtggtcgagc
    16141 tgcttgtccc agattccgaa ctggaagaaa tggttgttgt cgatggttcc gttcctgaat
    16201 cagcagatga aatacttcta ctcgtacttg tattttcttg tgtccgactg ctcgtcgaag
    16261 cagagctact gcttgacgtt tgcaacacgg gtgtaatctc cacactaact gattcgggct
    16321 tgatacttaa cccctcttct actggtattt caattttacg agttgttcga tgattgatac
    16381 ctgagacatt gatcggtaca gcaatactgt caccgatcga tgcaagcaaa tcgctagagc
    16441 cgcttacttc tgcttccagt tttgaaaaac tgaaatcata gctttctacc ccggcagctg
    16501 gtgttccttg ctgtatccca taaagtcgta ccgacttgct tggcttgatc gaatcagctc
    16561 tcactgttac ttgcactgga tcagagacga tacttaatac ttcccctgct gcattcagcg
    16621 cctgtattgg gacctcacta ttaatcccat catctgtgac catcgaagcg tccacatcag
    16681 ctactactcg tttgatttct gccaatgttt tgtcacctgt agtgatagag acttttttag
    16741 gactaacgga agtatcatca agttcatatc cagatggtgt gaacgttgaa ggaatcaacg
    16801 tttccacatc gaaatctttt attacctttt tctcaaccgt tactgtaata gtcgatggtt
    16861 cgatctttgc tgtgacagca ctgctcaaat tttgaatttt tagcggaact tcatgtgttc
    16921 cttcgcccag cttagtgata tctgctgtga cgcggaacat ccgtgtatct tcgtttgcct
    16981 ctgcattcaa ctgaactcgg tttgcactgg aaagtttgac agtcactgca ttttcatatc
    17041 catggatata gtagcgcttt gaatcataag aaatcttgat cggcaccccc gtaactgttt
    17101 gattatacgc ttcatttcca gaaagcgtat ttggaacgga ctggccattt gcattgaagt
    17161 acagcgcaat actgaacagc aatgcaataa aactgtaaag aatgcttgtc cgtttctctt
    17221 ttgagatcat ttgcgatccc ctcccttagt cataccgtct aggaaagatt gaatcacgtt
    17281 tttcttgttt tttgttttct cttcagcaat tagttctcta cgcaagattg ccaaatattc
    17341 ttgctgggat aagccagcca tcagttcatt atctaacgtg atgctgacgt ccccggtttc
    17401 ttcagagaca acgatggtaa tcgcgtcact gacttcgctt ataccgacag ctgcccggtg
    17461 tcgagtcccg aattctttag ggatcaacat actttctgat agaggaagat aggcgcttgc
    17521 tactgcgatc tttccttctt ttacaataac tgcaccgtca tgcagcggtg tattaggaat
    17581 aaagatattg atcagcagct ccccagtgat atctgcatct aaagcaatcc ctgtctcaat
    17641 atactcatct aatccagtat gacgttcgat agtgatcaac gcaccgattt ttcgcttgga
    17701 catatattgg atggctttgt caaaagacaa gatcatcttc tcatcttcct gctgttcgct
    17761 ttttgattgt ttgaaaagcg aactgcgtcc aagatgttcc agtcctctcc ggatttccgg
    17821 ctgaaaaatc acgatagcag ctatcacccc gtacgtaatg acttggttca tcagccaaga
    17881 caaggtgtgc agaccaatga tttccgctac gattcggata acaataaaaa tagctacacc
    17941 tttcagaagc tgaatcgctt ttgtgcctcg cagcagttgg atcagtttgt atacaagata
    18001 ccagacaacc agaatatcaa gtatgttcac caaaaaattc agagagaaca agtcaccgga
    18061 aaacatttgc tgccaatagt ccaaattaaa aagttccccg atttgaaatg acataaaaag
    18121 cctctcttct cttttgagat ttcctctcta gtatatcata tgttttaatt ttagaggctg
    18181 atatgctcgt atttttcttg agaaaaacgc ataattttca agaaaattgc aagtatgcgt
    18241 tttatttttc tctgcaataa atatttttaa acttcagaga attattcatt atattttttc
    18301 tgttattttt cctttttttc ttttgtagct tgttccattt ttttacgtcg ttctgctctg
    18361 gcagctcttc tagcagctct tctttcttct gcttcaaggt ccgtcgtttt ttcttttcta
    18421 gtcttaacgg gtacatcgat agattcctct ggttcaagtc gtttcttgat tttttctaat
    18481 tctcccgtca gtgcagcata gttgtaaaaa cgtgttttag cgtcgtggac agtcttttca
    18541 aataaagctt ttgcaagatt tggatttgcg ttatctagcg aggaaaagcg aacttgtttc
    18601 ctcatgaaat caggcatagc agagaaatca ggttttttgt agtccaaagt cattgggttc
    18661 ttgttttttt cgaccattgt agggttatat cgatataatg accaataacc agaagaaaca
    18721 gcttcttttg cttctttaag cgattctttc attccgccaa gcaagccatg tgtgatacaa
    18781 ggcgtatagg caataatcag agaaggacca ggatactttt ctgcttcttc aaatgccttg
    18841 atcgtttgca tttgattggc acctgaagca atttgcgcca cataaacatt tccatatgtc
    18901 atcgccatca ttcctagatc tttctttgaa gcatactttc cactagccga aaatttagcg
    18961 attgccgaag ctggtgttgc ttttgaggtt tgaccgccag tattggaata gacttcgtta
    19021 tccaaaacaa ggatattcac gtctgctccg ctggcaagta catgatcgat cccgccaaaa
    19081 ccaatgtcgt aagcccatcc gtctccacca atcatccatt ggctgttttt tacgaataga
    19141 tcctttctct tgtatatatc ctccagttca gggaaaattg atcgttcgct ttcgatcgca
    19201 gcttgcagtt ttgctgctct ttgctgcgtt ccttcactct caaacatgtg tactaaccag
    19261 tcttccatca attctcgtag ttcttctgat acttttggca atacttcttt gattgaactt
    19321 gccaaagcct cgcgacgagt ctgtgccgct agaagcatac cataaccaaa ttcagcatta
    19381 tcttccaata aagaatttga ccatgcaggc ccttgaccac attcattggt cgtataagga
    19441 gaagcaggag aagcaccgcc ccaaatagaa gaacagcctg tcgcattcgc gatcatcatt
    19501 cgatcaccga acatttgtgt caataatttt acataaggtg tttcgccaca gcctgagcaa
    19561 gcgcccgaga attcaagaag cggttgattg aattgtgtag acaagacagt attctttcct
    19621 ttgactggat ttgctttttg tcttaatgtc attgcaaaag cccaattcac agcttgttct
    19681 ttctgttctt cataaggctt catcactaaa gctttttctt ttgcgggaca agcctctaca
    19741 cacagaccac aacctgtaca gtcttccact gaaacttgga tacggtattt tacgccgtct
    19801 gcgcctcgca tttctctcac gataaatcct tctggcgctt cttccatttc ctcttcatct
    19861 gctaaaaatg gacggatggc agcatgagga caaacaaagg cacattcatt acacatcgta
    19921 cagcgatcac taatccactc aggtacttct aaagcgacgc cccgcttttc catagaagcc
    19981 gttcctaaag gcatttcgcc agttgtcatt ccatttgcta caagtgtacc aacagagagt
    20041 tgatttcctt cctgtgcatt cactggttca agtatttctt gtatataacc agtgttcctt
    20101 ttattgattt tttcaggcat ctccaacgtt ctccaatggt ctgggatcgc gacttgatga
    20161 agcagttcgg tcgtccgatc gattgcttgt atgttttttt ctacgatagc cattgattta
    20221 tgtccatagc ttttcagggc ttcttctttt agcagcggaa gcacctcatc aaaaggaatg
    20281 atgtcagcta atttaaaaaa agccgtttcc attgccgtat tgatccggcg tcccaaaccg
    20341 acttcttggg ccaatctcat ggcattgatc gtatagaatc taatgttatt ttcagccaag
    20401 taacgtttta attttaaggg cagatgcgtt tctaattgtt cgtcgctcca aagagtattc
    20461 aataaaaaaa tgccacctgg cttcaatcct ttgaccaagt catagctatg caggtaagct
    20521 ggtgtgtgac aggcaacaag gtccgcatgc tcgacaagat acgctgaacg tatcggcgta
    20581 tctccaaaac gaagatgaga gacagtcaga ccgccagatt ttttcgaatc ataataaaaa
    20641 tagccttgcg catatttatc ggtgtgatct ccaataatct tgatagctga tttatttgct
    20701 ccaactgttc cgtcagatcc aaatccccaa aacttggcct ggaatgtttg cggttctgtc
    20761 aaatccagac tttcctttgg ttccaaagat tgataagtca catcatccac gatccctatg
    20821 gtaaaccgtt ttttgcgtat gcttgggtct tttttcaatt catcaaaaac tgctgagatt
    20881 tggtcgggag tcacatcttt agaccccaat ccgtagcgac caccaataac agccgggcga
    20941 agttcagaat catataacgc actttgaacg tctaataaga gaggttctcc acctgcccct
    21001 ggttctttac ttcgatcaag aacagcaatt gtttttactg tatgaggcaa cttttgcacg
    21061 aatgtttcca ctggaaaagg acgataaaga tggatattca aaaaacctgt tttccgtccg
    21121 tttgcattca agtaatcaat cgtttgttca atcgtctgtg caactgatcc catagaaaca
    21181 atgacttctt ccgcatcgtc tgctccgtaa taagtcacca agtcataaga agttcctcgt
    21241 aatcggttga tctctttcat atatttttga acgatttcag gtaatttatc ataatagggg
    21301 ttgatcgttt cccgttgttg aaaatggatg tctggattct ggttcgtacc actaacagtc
    21361 ggatgattgg gattcatccc tcgtttacga aatgaagcta atttctcctg attgatcaga
    21421 ggagctaatt cttcatattc caaaacttcg attttttgaa tctcgtgact cgtacggaat
    21481 ccatcaaaga aattcaaaaa agggacttct ccttcaatag aagcaagatg ggcaacaggt
    21541 gctaaatcca tcacttcttg tacactgctt tcagctaaca tagcaaatcc tgtttgtctt
    21601 gcagccatca cgtctccgtg atctccgaaa atattcaacg catttgtcgt cactgcccga
    21661 ctagcaacgt ggaaaacact tggcagtagt tccccagcaa tcttgtacat attcggtatc
    21721 atcaaaagca gcccttgtga agcggtatat gtagttgtca gtgcaccagt tttcaatgat
    21781 ccatggacca caccagctgc tcctgcttcg gattgcattt ctactacttt tactggttga
    21841 ccgaatacgt tttcttttcc tgctgctgac cattgatcta ctaattcagc catggtcgaa
    21901 ctgggtgtta ttggatatat tgcagcaatc tcggtaaatg cataagaaat atatgcggct
    21961 gccgtattgc catccattgt tttcattttt cgcatgattc atcatccttg ttcatattct
    22021 ctcttattat ctgttccatt ataacgtaaa acatgccatt tttttctttt atttataaaa
    22081 atattttaca tgtatctatt tgaattggtc atctaatttt tagacaaaca gtctaaatcg
    22141 ttgccttttt ttgtcagaca tgtataaact ataagcgaaa gaggtgtttt aacatggata
    22201 tatcagtgat tgatgcaacg aaaacaaata cacaaaaagg aatcctttat ggttcatcga
    22261 atgctcctaa aaaaatggtc gaattcatca acttagcttg tccgtattgc cgacaatggt
    22321 ttgaagaatc ttatgagcta ttagaagaag cagtccaaag cgggcaattg cagcgagtga
    22381 tcaaattgtt cgataaagaa aaagaaagct tacagcgagg gaacgtgatg caccggtatc
    22441 tcacaatcag cgatggccaa aaagcgatca aagagatcaa acaaatattc gatactcaag
    22501 acgagtggaa acatctttct ctccaagaag tagcggattt tgcagtagat aaactaaagc
    22561 tgacagaaca aaaagacgaa cagctgagcc aagctgtcat caacgaagct gagcaagcac
    22621 atattcgatt tgttccaact gtcatcttgg gtaaagaaat ctttgatgaa tctatttcaa
    22681 tagaggaatt aaaagaattg atccaaacaa aataaaaaaa taaggagcgt gggacaaaag
    22741 taaagaacac ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc
    22801 cttcttattt cttggagtta aacgcttctg tcccagcctc cttattccga ataaatggtg
    22861 tccaaaagct tgcatctgcc agattatctc atttcttatg acaagctaag caaattatct
    22921 ccaccaaata atttattcat atttttgact tgcgcatact aactaattct taaattttca
    22981 tacaaaattt ctcaatttcc cttaacgagt aatatttatg tacggtgatt ctcactagta
    23041 tcccaatcac tttttaagtt atcccgattt caattctact tcaaaaatag ttgcttttat
    23101 gtatgctgtt agttgattag atggtcattt gttgagctag gtcgtttatt ttaaacaagt
    23161 tgtttcttta gcttatacct atctcgtcta ccaaacgcaa gatggcaaac acaaatgcat
    23221 acgcatctgt atttgccact ttgattcgta taaagctaac aactattaac tcctctcagc
    23281 taatagttgc tctctttttt attcagattc atttttgctg atcacagcct agtatttcaa
    23341 tggttttgaa cactaaacca aattgttcca ttacaacatt cactggctga tgaaatggat
    23401 tagtagattt cgattattca aagcgatttt ctattggtgg atcgtgttcc caactaataa
    23461 gacggattcc aaacctatcg tacagatctt ctaaaccact tataaatttt gcacctattt
    23521 caggtgtttt attgcgtgga tattctattg ttacgccatt aaatggatac caattaggat
    23581 taggctctct ttttttctgt acatctccca tagaatagtt agcactttct gggacataat
    23641 atattcttcg accatcctct aattcatcta ataagatatt tcgtgatcgt ctatcacttg
    23701 ctaaatgtag acgccacgtg cgataccatg ccatgctacc tgcatctgca gaaaatccat
    23761 caaataatgc aacaattgta tcttctatca caatcctttc cccatctact tcatattcta
    23821 atctaaaatc aaactcccct cgggtaattt ctggtcttgg tggcttagga cccaatatta
    23881 ttctacttaa aaccgcattc gttcccagat gaaacacagc tataacaaca ataatgatta
    23941 atattttgtt tctatttgat aaatttctca ttattcgcac atcacctcta cattctcaca
    24001 tattagttcg taatactcct ttaatatcaa aatatctact ggtcgtaata atattcgcta
    24061 tgaattccta ctactcgtat aagaaaggcc aaatcatcag cttttacttg ctgatgattt
    24121 ggcttgtttc tatttatcgt aattgttttc taatcaccaa aattgttaac aatcggttca
    24181 gatatttccc agttgattaa ggtaattccg tattgtgtca aaacttcttg cgcttctccg
    24241 aaattaagta cagttcttat gtatccactt tctcctaata taaagtctac ctctctttta
    24301 aaaccttcct cgagatttct aagggctata tgtggtttta atgacattga ttgtacattt
    24361 tcccccatat aatattgcgc acttcctcga ctaaaataga ttcctagatc gtccccaaaa
    24421 ggtagatctt ctctaccact agctaatcta cttctccatc ttcgttctct tttcatagaa
    24481 cctgcgctga actcacaccc gttaaactca gctatgacag tatcttcaat tatgttaatc
    24541 tgtccatcta gttcgtattc aatcctaaat ggaaattctc cataggtgat ttccggttcg
    24601 ggtattttag gtgagattaa tgcaaagaaa ggaaatatta agcttggcaa catcaaaaaa
    24661 actcctccta tgatcacaat tcccgatgta atccaaaata taatttgtac aattctcatt
    24721 gatccattgt attccatttt attcccccta ttactattca tatccgccta tctatatcga
    24781 tcttccaatg cagagataat tgcttccata ccttgatttt ttactgagtt acctatgcga
    24841 gatatcattc tccactaagg tgcatcatat tgtctatcta acatgattaa ttctccaata
    24901 ggtgtatact cctccaaaaa caacatttaa tgcatctccc tcgacaacat aagctaccat
    24961 gtttgctgta tatgtgtttg agtctaattc ataagcagat aaatttactg tcgctggatt
    25021 aaatataatc gcattcgtat ttatagctac tgaatttgct gcttcagcac caccttttga
    25081 atgtccgatc attgtgattt caatagtagg atttacgtgg acagccctca ccgtttaccc
    25141 tgaccattat agaaatcgct tacttttttg aactacgcaa caaaggatac aatgctccta
    25201 acttcactgt ctccattttt gtccactaag gaatataaaa aataggcatt aattcattta
    25261 attcggtcac aacacgttac cttttaaatc attatactgt atctcataca ttattatcaa
    25321 taaactatta taaaaatgca aaaaatgctt tatcaatcta ttttgtaatt ggtcataata
    25381 tcattgaaat cataccctgt atcgattttt cctatgaatc tcgtctattt gtgattgacg
    25441 aaaaagaggc aagacagaca ctggaatggt aattttaccc actattttgc tccaactatt
    25501 tgtaatcgta cccacttttg aaataccgag atgatcattt taattgatat gctgattccc
    25561 aagcaaaacc ccaagaaatt aaatgctcta tcgcatctag cttcttgggt tttgcttata
    25621 taaattccaa catttaaatt ttttatgctg ttcggtctca gaaatgccgt ttttgcttat
    25681 atcacaatat caagatgtca aattattcgt gttgccgatg actttgatta atttttttca
    25741 aattgtttat taagactaat gctgtctttt tgcagatacc aatgttcgaa ctttttaaca
    25801 ttatcatatg atcctgcaag ttttaacttc ttaccattag atgttatcaa agttataatt
    25861 aaccttgcct ttttcccatt aggatctttt attttatgaa ttttctgcag gtaatctctc
    25921 ctaatggcag ctatttttac ctcatcagtg ctgataaacc attgatcatc cacatacata
    25981 ctgcctgtta tgttgtgttt ttccatttct tccgagaacg aaaagtttaa ggctcgttct
    26041 tacttccaaa gttgaatgag tccatggatt ggacttagtg tgagaattga gaacaacaat
    26101 aagaatccat atcctcccgc aaacatccct aaggctatac tgtctctcca tccataagta
    26161 ggattagaag agaaaagagt caccgtttgt aataatatga aaaggaataa tccagcacaa
    26221 acaccaaaga taacaactcc aaacattgaa tttttataca atgaccattt atttataggc
    26281 ttttctttca ttttacttcc ccctactgtt ctgttcacac ggaatgaatt caaagttcat
    26341 aagccctcct ctttgaacat atgaatacga cttgctaact atactttaaa aaagcttgtt
    26401 ttaagcggtt ataggcgagt tgtaatctct attcatctca ttagcaaata acttgccgaa
    26461 aagtctactg aaattaaagc agatagtgga agaattattt agccaggata gacactattt
    26521 gtgaaacaca tgtctttact tttgaaattc tatgatgaaa agccttggaa tacagtagct
    26581 cttaattggt aatacttttt ccattataaa taacctcttt tcattttata tttatcccct
    26641 ctaaaaataa tatctaatct aatattttaa acttatattt ccctcgctgt gctaatagag
    26701 cattactaaa taaatagaaa ctagaaaaaa gcattacgcc tgccagaata aaccaattat
    26761 ttgt
//
LOCUS       DANKEF010000035        24380 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000035 DANKEF010000000
VERSION     DANKEF010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.035"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(60..1613)
                     /locus_tag="PWZ48_001690"
     CDS             complement(60..1613)
                     /locus_tag="PWZ48_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2261347.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            1761..3050
                     /locus_tag="PWZ48_001691"
     CDS             1761..3050
                     /locus_tag="PWZ48_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2261348.1"
                     /translation="MRYSTILISTFAALSLSLTINQTVHTEEIETAKWTETKWANVNR
                     ITFQDEFSSFNSGVAKLRKGQTATIKAKIDYSGDLAPILQSAVTFENVDPALSIGYDE
                     NTLTIQHKQAIFTFTVTLNQDLLKPALFTVKVSDGLPNDHHHLTPYSQRQTIEQDSAI
                     DSGGDLVEEPTDKPENENKPEVPPTENPDGEQKPEIEPGEEPDTETQPEPDNESKPEI
                     TPGEKPDVDPEEKPDVTPEPDTDSGNQTVPETNPDTDNETENPEKPEVAPEEKPDVTP
                     EPDTDSGNQTVPETNPDTDNETENPEKLEVDPEEKPDVTPEPDTDARDQGIPEKINKK
                     TIQEDGKKESKKSNLAILKINEEQLNKKSRIFDSAQSAETLKSSKDTTFASPETKNKQ
                     LPKSGESQNKVIMWSGIILLSIATMLSAKRFKQNRSL"
     gene            complement(3176..4255)
                     /locus_tag="PWZ48_001692"
     CDS             complement(3176..4255)
                     /locus_tag="PWZ48_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2261349.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVIDFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVIALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(4364..4804)
                     /locus_tag="PWZ48_001693"
     CDS             complement(4364..4804)
                     /locus_tag="PWZ48_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2261350.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTSLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTPQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(4861..5271)
                     /locus_tag="PWZ48_001694"
     CDS             complement(4861..5271)
                     /locus_tag="PWZ48_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HDL2261351.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(5452..5886)
                     /locus_tag="PWZ48_001695"
     CDS             complement(5452..5886)
                     /locus_tag="PWZ48_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2261352.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVSFEERKQLFEKYM"
     gene            complement(5986..6834)
                     /locus_tag="PWZ48_001696"
     CDS             complement(5986..6834)
                     /locus_tag="PWZ48_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HDL2261353.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAVLGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLFFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFVFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            7223..8989
                     /locus_tag="PWZ48_001697"
     CDS             7223..8989
                     /locus_tag="PWZ48_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HDL2261354.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETRRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVRDHLDYLTDLGVNGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVYDREDKLYFTRELEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            9091..10350
                     /locus_tag="PWZ48_001698"
     CDS             9091..10350
                     /locus_tag="PWZ48_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2261355.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            10497..11798
                     /locus_tag="PWZ48_001699"
     CDS             10497..11798
                     /locus_tag="PWZ48_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2261356.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            11801..12652
                     /locus_tag="PWZ48_001700"
     CDS             11801..12652
                     /locus_tag="PWZ48_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2261357.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            12665..13480
                     /locus_tag="PWZ48_001701"
     CDS             12665..13480
                     /locus_tag="PWZ48_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HDL2261358.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            13631..14797
                     /locus_tag="PWZ48_001702"
     CDS             13631..14797
                     /locus_tag="PWZ48_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2261359.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            15027..15674
                     /locus_tag="PWZ48_001703"
     CDS             15027..15674
                     /locus_tag="PWZ48_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2261360.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            15758..16702
                     /locus_tag="PWZ48_001704"
     CDS             15758..16702
                     /locus_tag="PWZ48_001704"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HDL2261361.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            16837..18288
                     /locus_tag="PWZ48_001705"
     CDS             16837..18288
                     /locus_tag="PWZ48_001705"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HDL2261362.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            18309..19145
                     /locus_tag="PWZ48_001706"
     CDS             18309..19145
                     /locus_tag="PWZ48_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2261363.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            19145..19804
                     /locus_tag="PWZ48_001707"
     CDS             19145..19804
                     /locus_tag="PWZ48_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2261364.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSIHQS
                     I"
     gene            19930..20619
                     /locus_tag="PWZ48_001708"
     CDS             19930..20619
                     /locus_tag="PWZ48_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261365.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PKDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            20625..21581
                     /locus_tag="PWZ48_001709"
     CDS             20625..21581
                     /locus_tag="PWZ48_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HDL2261366.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            21625..22137
                     /locus_tag="PWZ48_001710"
     CDS             21625..22137
                     /locus_tag="PWZ48_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2261367.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSGVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(22393..22941)
                     /locus_tag="PWZ48_001711"
     CDS             complement(22393..22941)
                     /locus_tag="PWZ48_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2261368.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(23030..24211)
                     /locus_tag="PWZ48_001712"
     CDS             complement(23030..24211)
                     /locus_tag="PWZ48_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDL2261369.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
BASE COUNT         8222 a         4365 c         4636 g         7157 t
ORIGIN      
        1 cttgatacat aaatccggag gttgttaata agcattcgga tttatgtatc ttatttggtc
       61 taggaagtaa cttgattttc ttttttcgat tgatccatga gtgcttcttc ttttaattca
      121 aagatatatt ctgagatcct cataatcact tttttccagt cttctggtgt gatgattcct
      181 tttaaaatat agattttttt gatatgagaa ggcaattgtt tcaaagggca gtcgctgata
      241 acgatgttgc agttctccat aaaatcattt tgtattttga tcgattcatt tgaaaaaatt
      301 tgttctatct taacgatcag atctttactg atataaaaat cacgcgtata gttcacacca
      361 atcacaattc gcggctgttt tttgtagcga tcatacaaat gtactaggtc ttcaaccatc
      421 cattgcacat tttccttttc taaaataact ggtaacaagt gttgattttg ttctctgaaa
      481 tccatataaa attgttcaat ggcggtttgg acttctttca acacgctatg ttcttgataa
      541 agcatgaaag taggaagggg cgtatttggt tgattgaagt taagaaatcc attataaagt
      601 ttatttcgtg cgaatgccaa tgagtaaaga atcttttctt tttcaggtat aaacaaatca
      661 aatacagtac aaaaggactc caccatttct cttgaataaa gaacatgagg aacgtttgct
      721 ttttttaaaa gtttgtattg ctccaagctt gtttcttccg attcgatttt cgtgatagaa
      781 agtcgagcta aaatattcac taagatgcgt tgttcgcgac aaattggcag attaggtcgc
      841 aaaatatcta cagagggatc aataaaaaag tcaatatatt catactctgt gaattctttt
      901 tgtacgtctt tcacagaagt tgcaggaaaa ttctctttac atatttttaa tagaaaatat
      961 aatttatcta tcataccgct atttagtcgg tcaagtactt cttttttaaa aaaagtattc
     1021 atcataaaag ccactacttg cggatcttca tcataaaatt ctgtagcagt acttgaaaag
     1081 atgttccaat aaatatctaa tgctgcatat tgaatttgta cctcttttcc agaaaaagac
     1141 tttctgccat tattagaaaa ggagatagac aaaccgtaca atgctaaaaa tgcattaatc
     1201 tttttcattt tcgtatacaa ataagaggca ctatagtttg tttgctgact taaactgata
     1261 atcgaatttg tctgacagct gaacaaagaa gtcaagatct gataactgac tgattcgttg
     1321 cagtatcttc caaatagacg ataatagcag taagccggat caattccttc aaaattcaac
     1381 tttaaaaaag gtttttgctt atcaatatgc agttctgtta gattgagacg ctcaaagtct
     1441 tgttctaatt ctttgatgta tagtaaaaaa tttgattcag acatatggaa atgatctgct
     1501 aaatattgtg tagtaataaa gaaacgatgt tcagatagga aaagaaaaat atctaatttt
     1561 gtattgcttt ttttcttcaa gagaaacgtt ttaatcgctt cttgagtttg catgaaagag
     1621 gctccttttt acttaaattg ttaaaacttg aaaatattac tgtttttttc ataaatttag
     1681 acaaaaaaac agtaaacata taggtagaat ctctactatc ttatttcttg catacaaaaa
     1741 aatcaaggag tgagagagtt atgagatatt caaccatctt aatatcaaca tttgcagcct
     1801 taagcttatc gcttactata aatcagacag tccacacaga ggagatagaa acagccaaat
     1861 ggacagaaac caagtgggcc aacgtcaatc gaattacctt tcaggatgaa ttttcatcct
     1921 tcaattcagg agtcgcaaaa ttaagaaaag gacaaacagc gaccatcaaa gcaaaaattg
     1981 actattcagg agacttagca cctatactac aatcagctgt tacatttgag aatgttgatc
     2041 ccgctttgtc gattggctat gacgagaaca ctttgactat ccaacacaag caagcgatat
     2101 ttaccttcac cgttacatta aatcaggact tgttaaaacc agcgcttttt actgtcaagg
     2161 tatcagatgg actaccgaac gatcatcatc atttaacgcc gtatagtcag cggcagacaa
     2221 ttgagcaaga ttcagctatc gactcaggag gagatcttgt tgaagaacca acagataaac
     2281 cagaaaatga aaataagcca gaagtaccgc ctacagaaaa tccagacgga gaacagaaac
     2341 cggaaataga acctggagaa gaacccgata cagaaactca accagagcca gacaatgaat
     2401 caaaacctga aataacccct ggagaaaaac cagatgtaga cccagaagaa aaaccggatg
     2461 tgactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2521 cagataatga aactgaaaat ccagaaaaac cagaggtagc cccagaagaa aaaccagatg
     2581 taactccaga accagatacg gattctggaa accaaacagt accggaaaca aatccagaca
     2641 cagataatga aactgaaaat ccagaaaaac tagaggtaga tccagaagaa aaaccagatg
     2701 tgactccaga accagatacg gacgcaagag accaaggaat accagaaaaa ataaacaaaa
     2761 aaacaattca agaggatgga aaaaaagaga gtaaaaaatc aaatctagca attctaaaaa
     2821 taaatgaaga gcagctgaat aaaaaatcac gaatatttga tagcgcccaa tcagctgaaa
     2881 ccttaaaaag tagcaaggat acaacctttg ctagtcctga aacgaaaaat aaacagctac
     2941 caaaatccgg agagtcacaa aacaaagtta taatgtggag cggaatcatt ctattaagta
     3001 tagcaacaat gctttctgca aaaagattta aacaaaacag atctctctaa catttgaata
     3061 atgtctgtga acgcattttt caaataaaaa gaaatatcca gaaaacgacc ccaaaatacc
     3121 agattgaaat ctgatatttg gggtcgttca tactttatct ttcttcatat ttattttaag
     3181 ggatatctgt ttcttttgga agatggttcg ggatcttttt cactttcaat acatctaaaa
     3241 agaaggtaaa gatcggaatg ccgacaatca agccccaaac gccaaagaac cgttcgctaa
     3301 caagtaagac cacaaatgta taaaaaatcg gcagttctgt tttgctggac atcaacttcg
     3361 gattcagcac ataagactca attagatgga caatcaaaat cgtaatcagg atataaacaa
     3421 catctcggat gcctccttgg gaataagcta tgaaagatag aggaatacaa gaaatgataa
     3481 cacctgctac tgggatcaga cttaaaatga aaatcatgat cgctaaactc agcagctgat
     3541 gaaagccaaa tacagccaag gcgattgtag tgagcaacgt gtttaaaaga gcgataacaa
     3601 attgagcttc taataccaaa ccaaatgtat tgacaaactt atcggcaaaa tagtaaatat
     3661 cttgaaagaa ccaagaaaat tcacttttta aaaacaatct tgagaataca attgtttttt
     3721 tcttttcaat cataaaaaag aaacttaaga tgaatgacat agcaaacgcc actgccattt
     3781 ttcctatatc ctgtaaatag ccaagcgcaa tcgaagcgcc attttgaagc tgttcaaaaa
     3841 aatcgtttcg ctcaatataa gtatggatat attgtaaaac aggatctgca tcttttggct
     3901 gatgctcgta aaaatcaata acggactgga tcatatcaaa tgtttgatga ataagcatcg
     3961 ggatatactt tgtgaatgcc agatagataa ggtaaacgat cagtgtataa atcatgatag
     4021 ctaaaacacc tgtagggatt cggacaaatc gctgaatgat ttgaacaaga tgaatcatca
     4081 aataagtaaa gataaatgtg agcaaaatgg ttgtaatcat acttctaact aagaaaagaa
     4141 caaaaacgac aaataataaa acagtgagac ggcgtatttt ttcattcgcc aaaaaacgct
     4201 gataaacggt caaatcgaca acctccaaat aacaaaaaat aaacacgtta ttcattatat
     4261 gatggaatat gaaaataaga aagagaaaga gcaaaaagaa aaaccgctaa atgacgttat
     4321 cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc tttttctggt
     4381 cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc aattcttcct
     4441 gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt ttttgcaacg
     4501 cagcaccttg aggagtcagc gtaacattta cgattctttc atcacccgtt tcacgctgac
     4561 gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta ccagaatcca
     4621 gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat aaaactaaca
     4681 tcacaagata ctgggtatag gtaagattca gcggcttgag gagggaagtg tagtgtctaa
     4741 tgatttcctt agaggcggca tataaaggaa aacatacttg attaactaaa tatagatctt
     4801 ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac acaaaaaagt
     4861 ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt ttgaatacgg
     4921 acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa gaccttcgat
     4981 agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat aaagggaaac
     5041 ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca attctaatgc
     5101 actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg ttgcattttc
     5161 gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat tatgaacttc
     5221 tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca taaaattacc
     5281 acctttaatt atattttata caattgaatt ttacacaata taattgtgga aagcaaagaa
     5341 atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt tctcgaatag
     5401 caaacatata caaaaaaata tcagagacaa aaactaatct catcataaac attacatata
     5461 tttctcaaaa agttgttttc tttcttcaaa acttacttca cgatggacct gatgaagcat
     5521 ccataaataa ccaaaaggat ctctgaacat tgagttagtc acaccaaagt ccatcatttc
     5581 tgtcatttct tgaatctcta cacagccatt tcgtaatgct ttattatgtg tgtcctcgat
     5641 atcagagacg atgatattga accagatcgt atttggatgt tcagcagtag gagcagtcaa
     5701 accaagagac gtgttttcat ccagcatatg gaatctcgtg ccatacaagg taaaaatgac
     5761 ttcattattg cctctagggt aatctgttgc ctcaatcagt tgtatttcat caaagattgt
     5821 ttgatacagt tctagtgctt tcaaactatc atttaataca aaatctattt ccgttccttt
     5881 atgcataaag atctcctctc cttttcagtc gaacagttac tagattcttt tattatacca
     5941 gttaaagaaa acgcattcaa aaaaatagcg gatttactta ccaactcatt cgattggatg
     6001 ataagtaaat ccgcgtccca ttacttctgc tacgctgctg acaatgacaa aggcacttgg
     6061 atcgatttct tctatcaatg gtttcattcg ggaaaattca tgatcagtca caacgatcaa
     6121 taaaacctct tttgactttc gattataccc gccttttgcc tctagcaggg taaggccacg
     6181 tccaatctca gcacttaagc gaaggcggat gtcttccaaa taatgttcac tcatgaccat
     6241 aacagatttc tttcgattga tacctgtttc gatataagtc atgacaattg aagtgattgc
     6301 aatagataaa atagcataaa gaaactcttc tattccaaaa acaaacagat taagagaaac
     6361 aacgaatgcg tctgtaaaaa ataagccaat cgaagtattc aagtggaaat attttttaaa
     6421 aatgagggga gggatcgtcg taccgccgct agaagagttg tttttatata aaatcgcaac
     6481 tcctaaagca aaaatcgcac ttccaaaaat gacagacaac aaccgatctg aagtaatcat
     6541 tgtttctgga actattgcaa tgattaaagg aaaaaacaag cttccgaaaa gtactttaaa
     6601 aaagggtttt ttacctaaaa atagaagagc tagtaccaac ataacgatat tcaacactaa
     6661 aataactata gctcggtcaa atcctaaaac agcttccagt aatattccta gaccgctgac
     6721 acctccagca gcaatatgat gaggccctaa aaacatattg atagcgactc ctaatatgaa
     6781 gagggcaaaa ataatcttaa tgtatgtcca tataaattgt ctcgtttctt tcatttatcc
     6841 acttccttaa ttcattaata atactcttat cttattataa cttacttttc accttaagtt
     6901 aactttaatt aataaaaaaa ttaggctagt ctaaattatt atgtttcatt cttctagaat
     6961 atacttctac gttaaaaact gttaaaaaac ttttacttta gaaaattgga gaaattgatc
     7021 gcttaagata aattcaggac actaaatagt acaggaagta gattaaaact tttaaaagtt
     7081 ttaagtctat ctatttggca agtttaaaaa ataaaaattc ggtatcaaaa aactgttacc
     7141 gataacattt ttgaaagcgt tgtcttttgt ttctctatca cttatagtga tgacgtcata
     7201 ttaaaacaca aggaggaaat caatggatac agctgcaatt tatcatcgac cggaaagtga
     7261 atatgcattt ctatatacga atacaagatt tcgtattcgg cttcgtacta ggaaaaatga
     7321 tatcaaaaaa gtatatgtgc tttgtggaga tccatatacc ataacaactg aaaaatggta
     7381 tcaaaaacaa cgaccaatga aaaaatgtct aagtaccaat gttcatgatt attgggaaat
     7441 tgaagtgacg agcgaaacaa ggcgcttaca gtatgcgttc catgttgttg gggaagataa
     7501 cacggactgt ttttacggtg atcaaggaat ttttccttat cgagaaaatg tgattactga
     7561 accaaatttt tattttcgaa taccttattt tcatcaaatc gatcgattca ctattccaga
     7621 atgggtaaaa gaaacagtat ggtatcaaat ttttcccgaa agatttgcaa acggggacaa
     7681 agcaaatgac cccgaacaca ctttaccttg gggaagtaaa gatccagacc gagaagactt
     7741 cttcggggga gatttgcaag gtgtgaggga tcatttggat tacttgacgg accttggcgt
     7801 caatggcatc tatttttgtc ccatatttaa agccacatcc aatcataaat acgatacgat
     7861 tgattattat gaggtggatc ccgcttttgg cgataaaaaa cttttaaaaa acttgattga
     7921 tgaagcacat aaaagaggga tacgtatcat gttcgatgct gtattcaatc atatgggtgt
     7981 ccattcacca cagtggcaag acgttctcaa aaacggagaa aaatcgatct ataaagactg
     8041 gttccacatc cgttttttcc cagtagattc ttatcagatg actgacttac cagaaactgc
     8101 tgaaaatatc ccatatgata cttttgcttt tactccattc atgccgaaac taaatacagc
     8161 aaatccagaa gtgcaaaaat atttgctgga tatcgcaact tattggataa aagaattcga
     8221 tattgatgga tggcgtcttg atgttgctaa tgaagtcgat catcattttt ggaaaaaatt
     8281 cagagaagca gttacagaaa tcaaaccaga catctatatc cttggagaga tttggcattc
     8341 ctctcaagct tggctgcaag gggatgaatt ccatgcagtg atgaattatg cattcacaga
     8401 ttcgattaaa gattattttg caaaaaagaa aatcacagct tcacaaatgg tcagtggaat
     8461 gaatcatcag caaatgcttt acagagatca ggtgaacgaa ggaacattca acttattaga
     8521 ttctcatgat actgccagga tcttgaccct ttgccaaggt aacaaagaat tgatgaagtc
     8581 tgtgatggcc tttatgtttc ttcaaaaagg gtctccttgt atctattacg ggacagaaat
     8641 agggatgact ggtgaagatg acccggattg ccgcaaatgt atgatatggg agaaggagga
     8701 acaagatcta gagctattcg gttttataaa agaacttgtt tctctaagga aacagttgtc
     8761 taagattatt tctgaaggtt ctactcagtg gctaatagta tatgatcgtg aagataagct
     8821 ctatttcaca cgggaactag agggacagat catctatgtt tactttaatc agtcaaaaga
     8881 accttgggta gtagaacagg aaaatgaggt gattcttagt caaaattgtc agttgttaga
     8941 agacgggaaa gcagagatac gacaatacgg atttttgatc atgtgttgaa aactgttacc
     9001 ggtaacaaca agaaagcgtt tgcaaatgaa gatttgtgcc cattataata agggtgtaac
     9061 agcacgttga atacacagga ggaaatgaag atgaaaaaaa gaagtgtgaa acagttgggt
     9121 ttagggttaa tctctttagg aatcttaggc ggattagcgg cttgtggcaa tggtaactct
     9181 tcagcagaag gggaagccga tgaaggaaaa acgttaacag tttccgttga ttctggatat
     9241 aaagattata taaacgaaat aaaaaacact ttcgaaaaag aaaacgatgt gaaaatcaag
     9301 ctagtagaaa aggacatgtt cgatcagttg gaatcattag cgttagatgg gcctgctgga
     9361 aagggacctg acgttatgat ggcagcatat gaccggatcg gtgcgttagg acagcaagga
     9421 catctggcgg aagtaaaact gggaaatgaa gcagcttacg atgaaacaga caaagcccaa
     9481 gtgacttatg atggaaaaat ctacggagaa cctgcagtaa tcgagaccct tgttttgtac
     9541 tataacaaag atttagtcga tacagcacca tcaacgttta aagatttgga gaatctatcc
     9601 aaagacagtc gttttgcttt tgagtcagaa gcaggaaaaa atacaggatt tttagctaaa
     9661 tggactgact tttattattc ctatggttta attgcaggtt acggtggcta tgtatttggt
     9721 gatgatggca caaatccttc agatatcggt ttgaataatg caggtgctgt agaaggaatc
     9781 tcttatgcaa cagattggtt caaaaatgtt tggccaaagg gaatgcaaga cataaaaagt
     9841 gcaggagact tcgccagcca gcaattcatg tccaataaaa ctgcagcaat cattgacggt
     9901 ccatggcaag cgcaaacata caaagaaaac aatatcaatt atggtgtagc caaaattcct
     9961 actctaaata atggacagcc ttatcaaccg tttggcggag gaaaaggatg ggtagtcagc
    10021 aattatgcta aaaacaaaga cctctctcaa aaatggcttg attatgtaac gaatcaggaa
    10081 aatcaagaaa aattctttga aatggtaaat gaaatccctg caaaccaaca agctagggaa
    10141 acagcaaagg ctaaaaacga tgaattaaca acagctgtga ttgaacaata tgaaacagct
    10201 caagcgatgc caaacatccc agaaatgggc gaagtttgga caggtgcaga aaacttgatg
    10261 ttcgatgcag catcaggttc aaaaacacca aaagaatccg cagatgaagc agtaaaaaca
    10321 atcagtgaag ccatagaaca aaaatactga ttaaaatgat aaccgtccat ttttataaaa
    10381 aatggaacag gtgcttcaga taaaagtggc aagagcgaga tggaaaatta tcttgctctt
    10441 gttgccatta tcttaaaagc agtttgcggt ttagcaagtt tagagggggc attcagatgc
    10501 agcaagataa aacaagcaat atcaacgaaa aaaaaatcac gctttgttca cttattccag
    10561 ggatggggca gtttgtaaat ggccaagctt ttaaaggtat cgtttttttc gccatattcg
    10621 tcctattcat tggtcagatg cttctgggcg gatttcatgc attggtagga ttggttactt
    10681 tgggaagtat accaatggaa gatcattcat tatttatttt gattgaaggc actttgcaat
    10741 tcatcattac aatcattttc ttgatttttt acggtttgaa tcttttagac gcttatcgag
    10801 tagcgaagat gcgtcaagag ggcaaaaaaa tcaatcggac gatgaaggaa gtcatacata
    10861 atataggtga tgaaggtttt ccttatctat tgacacttcc cgcttatctt ctcatgattt
    10921 ttacgatcat tttcccagtc ctagttacat tgttcactgc atttactaat tatgacttca
    10981 atcatattcc accagccagt ttaattgatt gggtaggggc taaaaacttt tttagtatct
    11041 tctttttaag ctcatatcgt aatacttttt ttgcagtctt ttcgtggacc gtgatttgga
    11101 ctttatgtgc aacaactttg caaatcaccc ttggtattct cacagcggtt gttgctcacc
    11161 agaaatttat taaattcaaa cgtattttcg gtatcatttt cttattacca tgggcggtcc
    11221 cagcttttat tactatcatg agtttttcga atatattcaa cgatagcgta ggtgccatca
    11281 atactcaagt gattcctttc ataaaccagc ttccttttgt agatatcagt gcaatctctt
    11341 ggaaaacgga cccgaactgg acgaaagtag caattatctt agtgcagggc tggttaggat
    11401 ttccctatat ctatgtgatg gttacaggaa tcttacaatc gatttcagaa gacttatatg
    11461 aagcagcaaa aatcgacgga gcaaatgcat ggcagcgctt ttcaaacatc acgcttcctg
    11521 ctgtcttcct agtggcagcg ccaacattta ttacacagta tacggggaat ttcaataatt
    11581 tctccatgat ctatctgttt aacaatggtg gtcccggaag cgtaggcgga ggagctggtt
    11641 ctactgatat tcttatttcc tggatctata aacttacgac tggaacttct ccgcagtatt
    11701 ctatggcttc agcaatcacg ttgattatct ccgtcatcgt tatctgtgtg tcattgctgg
    11761 tatttaaaaa gacaaatgcg ttcaatatgg gggattaaca atgaaaaaat ttcattcgag
    11821 tgctcgattc caaaaacgac tttctcactt ttttacttac ttttttcttt taatactgtc
    11881 agtcattatc atctatccgc ttttgatcac agcaagtact gccttcaagt caggaaatat
    11941 tgcagctttt agtttagatc tttcaagtga ctggacttta ggaaacttta ctcggctatt
    12001 cagtgaaaca ctttacggga cttggtataa aaatacattg atcatttctg tcctaacaat
    12061 gatcgtacaa gtgactgtag taactttggc gggctatact tatagtcgct atcgttttat
    12121 cggccgcaaa tcaagtttga tgttcttttt agtgattcag atggtaccga ctatggcagc
    12181 attgactgcc ttttatgtca tggctttctt attagaagcg ctcgataagt actggttctt
    12241 gacaatgatc tatataggcg gtggaattcc aatgaatact tggctgatga aaggatactt
    12301 tgacactgta ccaatagatt tagatgaatc tgctaaactt gatggggcag ggcatttcag
    12361 gatttttttc cagattgtat taccgcttgt caaaccgatg ctcgccgttc aagcattatg
    12421 ggcttttatg ggaccttttg gggatttcat gttagctaag ttcctgcttc gaacacctga
    12481 aaatcagact gttgctgtcg gcttacaaac atttatttct gatgtgacaa accaaagagt
    12541 ctctcttttt gctgctgggg caattttgat tgcactaccg atttgtctat tattcttctt
    12601 tttacaaaag aactttgtta ccggcctgac atctggcgga acaaaaggat aggagcagag
    12661 tatcatgcaa acatttccgt taaactattt ttccgcatta cgtacgccaa ctcaagtgtt
    12721 tgcgggacgc aaactattat catggcctaa atttttcttg atatttgtat ttttagtttc
    12781 cttgatggtc atgcctgtga ctctttttta tgccaatcag atccaagcga ttccattaga
    12841 acaatttcta tccgttcatt cgctgatcga tgaacaagga acccaaaaat tttctgaact
    12901 tgaattatct gaaactgggt tacaagcatc gcaacaaacg atagctgtga cccctgaaat
    12961 tttggtaggt gtgtctttat ctgagaaaca acaatccgat catggaactt ttattgattt
    13021 tgagaaggaa caatgggtga tccaacaaaa ggataaatct ggtatacgcc gctatacgat
    13081 gaattatagc ccatctttcc aaccagattc tgtacgtacg cccgaagatt tccaacgatt
    13141 tttagaaaga gaattttatg ccagtaatcg tccaacaatc attttatctt atagtttaag
    13201 tcttggtctt gtcctatttg tcatgacgag tctcatttta ttcggggctt cctttttctt
    13261 gtggatgaca agaaaaagcc aactttcatc cattcataca ttcaaagaga gtgcaaattt
    13321 gatgctgaat gttatgggaa taggttccat ggcagcagca gttgtagggt ttatccactt
    13381 tgactttatc ttgatgctaa gtgtccaaac aacgataact gtcttgctgc ttttatgggt
    13441 ttttgtcaaa acaagattca aagataagcg tgtcgaatga caagtttaag cgatttatgc
    13501 gacaatgaat gtgaaaaaaa tgaaaaataa agtagttttt gaaaaatttc tgaaaaaatt
    13561 aaacttctta gttgattctt ttctttttta gtattaacgt aatagggtag tttttttaag
    13621 gaggaaaatc atgttagaga aaactttata caatcaatta ttttcacgtt ctttctcgct
    13681 ccctgtcgaa gtaaattatt gggacggtac aacgaaacaa tacggagata cggacactcc
    13741 tccccaaatc aaaatcaata tccatgaaga aatcccaatg aaagagatta caagtaacgc
    13801 ttctttggca ctaggagaag cctatatgga tcatcgaatc gagatcgagg gaagtatcca
    13861 agcattgatc aacgatgctt atcagcaagc aaacagcttt ttacgaggaa aagattatct
    13921 aaaatggctg ccaaagaaag aaaaacatac gaaacaaaaa aacaaagaag atatccatgc
    13981 gcattatgac ttaggtaacg acttttataa actttggcta gatccaactt tgacttattc
    14041 ttgtgcttat ttcaatacac ctgaagatac gttggaacaa gcacaaatca ataaagtcca
    14101 tcatatctta gacaaactct ttatcaaaga aggcgaaacg ttgcttgata tcggttgtgg
    14161 ctggggaaca ttgatgttca ctgctaccaa agagtataat gtcaaggcaa ctgggatcac
    14221 actaagtgaa gaacaatacg atttgattac caaaagaata aaagaagaac atttagaaga
    14281 taaatgccgt gtattattaa tggattaccg cgaactaaaa ggcgaaacgt ttgatcatat
    14341 cacaagtgta ggtatgttcg aacatgtagg ttctgaaaat cttgaaggtt atttcaaagt
    14401 ggtaaaagat ttattgaaac caaaaggaac agctttgatc catggaatca gccgacaaca
    14461 aggcggtgcg acaaatgctt ggatcaataa atatattttc cctggcggtt atgtccctgg
    14521 tgttactgaa ttagttgata atattacaaa aaacgatttg caactgattg atttggaaag
    14581 cttacgtcgt gactaccaat tgacattaga acattggaca aagaattttc atcaagtcca
    14641 agatcaagtc acgaaagaaa tgggtgattc cttctatcga atgtgggatc tatatttaca
    14701 agcttgtgca gcttcatttg aatcaagtaa tatcgatgtg attcaatatt tgcttgttca
    14761 acctggaaac aatgacatcc caatgcatcg tgcataattg aggcaaaagt actgtttaaa
    14821 gactatttaa aaagaagcta agttttatga tgctccagaa gtgaactttt ttgtctaact
    14881 tctaggattg tctctaaagc ttagtttctt tttctttata ttttcataat aaaagtttgg
    14941 ttctttaatt tttaaacatg acagaaaatt ttttcttcgt tatacttgta atagaatact
    15001 attttataac aaaggggatc gaatcaatga aaaaaactgt tatttatact agtttatttg
    15061 cagctttgtt tttagctgct tgtcaatcaa ataccaatca aggcacatct gggaatacgt
    15121 cttccagtag cgaacagcgc tcttcacagg tatcggactc ctcaacaaaa gcaagcacat
    15181 cttcgtctag ccagacaaaa agttctagtg aagcaagtac tcagtcagat atcaaagtgt
    15241 ctgcagctcg tgcgatcgaa ttgttccaag ataaataccc agatactgca attactagtt
    15301 tggaattaga tagtgattgg ggatcttact tctacaagat agaaggtgtc gatgatcaaa
    15361 aagaatacga agcaagaatt gatgctcaaa atgaaaaaat ggagacagag aatccagaaa
    15421 ctctagatcg agatgaacaa aatggtcaaa agaaaaaaga agatggtctg gatgttgata
    15481 aattgatcac gattgaggaa gcagcaaaaa tcgccgtcga caaagtagga agcggtacgg
    15541 caacagattg ggatttggat aaagatttag gtactactta ttgggaagtc aaagtaaaag
    15601 acggcaatca aagtacatca gtcaagatca atagccaaac aggcgaagta ctgtcaactg
    15661 aacaagatga ttaagaattt cttcggaaaa caagagttga tttttctttt agtcatgata
    15721 cactagacaa aataacttgt ttgaaggagc gatttacttg agtttagaaa cgacctttgc
    15781 caatcatatc tttgccaatc ctttgatgaa tgcatctggt gtccactgca tgactaccca
    15841 ggaattagat gaactggctc attcagaagc aggagcattt atcactaaga gctgtacgat
    15901 caatgaacga aaaggaaatc ccgaaccacg ctattttgat gttcctttag ggagtatcaa
    15961 ttctatggga cttccaaact taggtttttc ttattatcta gagtatgctc ttgcgtatga
    16021 aaaagtgcag gaaaaccaaa atcaaccgtt atttttttcc attgccggga tgagtgtcca
    16081 ggaaaacttg gaaatgcttg agaaaataga aaaaagtggg tttaatggga tcactgaatt
    16141 aaatttatct tgtcccaatg tgcctggaaa accacaacta gcttacgatt ttgaggccac
    16201 ttatgaaaca ctgaaagaag tattcagcat cttttctaag ccgctaggaa tcaaattgcc
    16261 tccttatttt gattttgctc attttgatca gatggctgat atcttgaatc agtttccatt
    16321 gacctatgtc aatgctatca acagtgtggg caatggtttg tatatcgata cagaacaaga
    16381 agcagtagtc atcaaaccaa aagaagggtt cggcggcatt ggcggagagt atatcaagcc
    16441 gactgctttg gcgaatgttc gcgcttttta tacacgtttg aaaccagaaa tccaaatcat
    16501 tggtacaggt gggattcgga caggacaaga tgcatttgaa catctgctat gtggcgcaag
    16561 catgttacag attggtacag agcttcataa agaagggccg gaaatttttt ctagaatcat
    16621 aaaagaatta acgcagatta tgtcagaaaa aggatatacg tcaattgacg aatttaaagg
    16681 caaattacgc accatttcat agcaaagaga cgaaggtcat gattgatctt cgtctttttt
    16741 tataaaaaac actcgtctgt tcattcctgt gcaagcgtac tcattgtatg ttagacgaac
    16801 ttgtgggaaa ataacagagg caaaaaggag gataatatga atgatgcaac agaattagca
    16861 aaagagctta aagaaaaaga atttagtttt caagaactag ttacggatat acagaaaaaa
    16921 ataaagaagc aaaatcatga attgaatgcg tttgtcgcgc ttgaaccatt cgagagtaat
    16981 tcccacgagc tattgattcc ccagtcagaa aaagggccat tggctggtat cccatttcct
    17041 ttaaaaatgc tcggtcaaga aaaaaaggga tggctagcta cttcaggatc tcgtttattt
    17101 gaaacacacc gagcttctca tacatctaat tatgtgcggc aagcagaagc gataggtcta
    17161 gttccttttg gacaaaccaa tgcgccagag tttggtttca aaaatattac agacccagtc
    17221 atctatggcc cagcaagaaa cccttggaac cttgaatgta caccgggcgg atcaagtgga
    17281 ggagctgctg ctgcagtttc atcaggaatc gttcctttag ctggtgctag tgatggcggt
    17341 ggttctatta gaatacccgc ttcgttttgc ggattgattg ggctgaaacc ttcgcgaggg
    17401 acgatgccag ttggtccata tgcttggaga ggatggcaag gagctgctat tgatttcggc
    17461 ttgacagttt ctatgagaga cacggaggct ttattttatg ggatgagaag tatccattcc
    17521 ggtgcgccat atcaagtatc gccagttgag tggcaaacac atccaagaaa aaaacggttg
    17581 aagatagctt tttgtgttga ttcaccgatc cactcacaga ttagtgaaga ggcagtactt
    17641 gctgtaaaaa aagctgtaga tttccttgga aaagaagggc atgatttgac agagattcct
    17701 tacccactag atggtcgtaa actgatcacg tcttattatc agatgaatgg cgcagaaaca
    17761 gcagcaatga tccgctcgat tgaaacaggc atccagcgag ctgtcagaaa agaagaactt
    17821 gaacccattt cttggacttt gttccaatat ggtcagaagc tctctgccgc tacttacatc
    17881 gattctctgc atgtatggga tgaagcagca attgtcatgg aaaacctaca tcaagaatac
    17941 gatctattcc tttcaccaac gactgcattt accgccccta aaatcagcga agatttgcaa
    18001 agtgatgcga taagagcaca gatggaacac atcagtgatt gttctgaagt agaaggttta
    18061 gacgttgttt cttccatgtt tgaggagagc ttgaagatca caccttatac acagttggct
    18121 aatctgactg gacaaccagc aatcagcttg cctactcatt tgacaaaaga aaacatgcct
    18181 ctaggaatcc aatttatggc tgccagagga agagaagatt tgctatttca aatcggctat
    18241 ctatttgaag caaatcatca gttccattta ccaaatccat ggaaatagca gaaacaagga
    18301 gaagaagaat gaagaaaaaa agattgtttg ctctaataac tagtgccttt tttattttaa
    18361 gcgcttgcgg tacagctgcc gctaatcagg aagataacag ctggagtaat gtagaaaaaa
    18421 agaaagaatt agtagtagcg acttctggca cactgtttcc cgcatcttat tataatgatc
    18481 aaaatgaatt agttggttat gatgtagatt tggcaaaaga ggtggctaaa cggttaaatg
    18541 tcaaaatctc ctttaaagaa tacaacgtgg atggacaaat cacttctgtt tcaaaaagag
    18601 aatcagattt tgctgcgaac gattttggta tcacaaaaga aagagaaaaa aaattctccc
    18661 tttcagagac tttcaaatat tcatttgaca gtatgatcgt acgaaaaaaa gatgattcag
    18721 gaatccactc acttgaagat cttaaaggga aaaaagcagc tggcgaaccc aatactagct
    18781 atatgcgttt agcggaaaaa cacggagcta aattagtcac atatgacaat gccacgaatg
    18841 atcagtattt aagcgatgta gcaaatggtc gaacagatgt tattctaaat gattattatc
    18901 tgcagaaaat ggccgtatct gctttaccag atatccctgt aaaaatactg gagaacgttt
    18961 atttcaatga aaacgaaagt ggttttttat tgaaagaagg aaatgatgct ctgaaaaaga
    19021 aaatcgatgg cgtattaaaa gagatgaaag aagatgggac gacaaagaaa atatctgaaa
    19081 aatattttca aactgatgtt tcagtcaaac cgaaagaaac gatggctgag tcggtgagtg
    19141 agtgatgtac tttcctctaa ttgatttttc cctcatgtgg acttcacttc ctttcgtact
    19201 gcaaggcttg gtatacacat tagggattgg cttcgtgtcg tttcttttag gaaatttggt
    19261 agggttgctt ttgacagttt taggtctttt ggattggctg cctctgaatg tatttattcg
    19321 tttttattta tcgttcttta gaggcattcc cgcactagtc ctgctgtttc tgttatattt
    19381 cgggttacct tatcaattaa gtgccttaac ggcttcagtc atctgcttta cgattaccag
    19441 cagtgcattt attggtgaaa tctatcgagg atcactagca ggcgtttctt caggtcagtg
    19501 ggacgctggt ttagcttctg gtttttcttt ctttggggta ttgaaatata tcgttttgcc
    19561 gcaagctttt cgaatctcag taccggcact cagtaacgta gctatggatc tagtgaaagg
    19621 tacttcgtta gcagcaacaa tcacagtgcc agaaatattt caaaaagcaa agatcatagg
    19681 cggtcggaca ttcgattatt tttctctcta tattttagtt gcactgattt attgggtcat
    19741 ttgtatcggg atcgaaaaag tgcaacagta tttagaaaaa cgttctatcc atcagtcaat
    19801 ctgaaaaata gaaatggtgc tagcgtggct ggcaccattt ctattttttg tttcttgtat
    19861 attctgccta gtattttaaa accatttcat gtaaaatgta aatcgaaaat atgttcggag
    19921 gtagaaaaca tgaagacccc gctaaccatc gaaatagaat cctcgttata ccaatattgc
    19981 atcgagcaag gcggcttggt tgtagaagaa gtcacgatgc ctgatgacca aggaattgta
    20041 gatacgctta gctgcttgac aaaatttgat ggaagccgag aatggcgatg ctatgagctg
    20101 aaagtgacaa aagcagattt tcgctctaca gccaaactgt catttgttgg acattataat
    20161 tattttgttt taccaagtaa actatatgaa gaagtaaaaa ctgaaatccc tacaggaata
    20221 ggtgttttag tgtatcgtcc gtacacggtt gaagaggaga tgcctgcctc tggtacattt
    20281 ctgattgcga aaaaagcagc aaagaaagag cttgctgtac cggaagaagc tttgacaaac
    20341 cgcttcatgg cttctttatt cagggaagta agaaaagcaa agcaaatgga gcgaggcgta
    20401 aaatattttc caaaagatca attgtacaaa gaattgaaaa gacgttcaga aaatcaagat
    20461 ccattttcta acgaacgata ttatgaacgg tttttagagg aaatagctag tcaggcaatc
    20521 aatgagttgc aagaagaagt tatcgcgctt cagcatgatt atgaatattt gaggcaacaa
    20581 cagcagatcc gtcggttgcc tacagagcca ttggagtgaa aaatatgtac tatccttatt
    20641 taagaggaaa acaatttgac ctattagcat taaaagaagc actgtcgcga ggattgctct
    20701 ccaataaaat ccaaccagtg atcgaaccag tacgtgattc cgctacgctt aaaaatgtga
    20761 tcgaattatt tcaaaaaaag caccatccaa tcgctgtgat ccaaaatccc caagtaggac
    20821 aattcaaatt attcgatcag catgtgcaca gttggacagt caaagaaaat agctctgtcg
    20881 tccctgcaca gatcttaacg ccagaaaatc tcagtgaagt actggattca cctccagcgt
    20941 ttcttgtttt cgatggacag cactacccaa aagatacaga ggtgtggaaa cagttggcag
    21001 gagttgattc gaaatttttg ataccagata ctagtcgctt tcgcatctgg ctgcccgaaa
    21061 acaaaattgt gatccgtgac tcttttcaaa cgagaaagca tgttgaaagt tatgcagata
    21121 aaacagatga cttttttagt gatgactatc tttttttcca cgcagacgga tatattggat
    21181 tttctgattt cacgatcgaa ggtagccgtt atttcgataa agggttccct agccgagcgc
    21241 ttgctatcca tctaacgtat attgatgctt acggcaatat acgcgtgaaa cattttgtct
    21301 ctgacagtaa cgactctgct aaagaccaag cattgaagtt tcttgaagca ggagataaaa
    21361 tgaaaaaatg gatcatgcgt caccatcatc aactcttgat tacttcagga atgattgaat
    21421 tattggctct ttaccagcaa caaaaattcc ctggactggg agtgttgaaa aagtggtcac
    21481 ttatgcatca tttggaactt attagtcacc tgctagatca tccaaaagat tggcttaaat
    21541 attattcaaa agttcaggaa ctatatgaag tgatccaatg attagataat gtaaatgaaa
    21601 aaggaataga aaagaggaaa ctggatgtat attcggcaaa caaggaaaga agaagtagaa
    21661 gaagtggtag aaatcattaa tgccgcagcg gatttcattg cttcgcaagg ctctcctcaa
    21721 tggcaaaacg ggcagaaacc aacaaaagaa acgattttga acgatattga aagaaaagaa
    21781 agctatgtat taatagtgga agatcaaatt gctggaacag ctgcattagt ctcaggcgtg
    21841 gatcccgttt acacaaaaat agatggtagt tggaaagaat caactggtcc ttatttatcg
    21901 attcatcgtg tcgctttagc agaaagtact cgaggaaaaa aactagcaaa accgttctta
    21961 gaacatttgt tgacgattgc agccagcaaa aatatccaag atatccgtat cgatacttat
    22021 ccagctaatg aaccgatgga aaagaccatc tattctgctg gattcactta tcaagggatg
    22081 attgaatttc cttttgaaca cggcgaaaga aaagcatatc aaataatcat caattgacta
    22141 taaaatcatc tgagcaaaga taaagacatt aacactactg aaaaaaacaa tcatatgata
    22201 gccaagaggt tgtaacagaa gtggccagct tcaaaaaaca agaaagaatt tccgaaaatg
    22261 cttctcaaat ttttgtggaa ttttagcgta ttttacgaaa tagctgcttt ctgaaactcc
    22321 cttcatcgag aggtcgtgac atagcttttg tcacgatctc tcggcttatc ctacgagtat
    22381 tattcgatac atttatatca ataatccttt ttttatttta tctgcagctt cttggaacgc
    22441 tccatcgttt gacacgacta aaagtgaggg gatagctttc aaacgtttca actgtgctaa
    22501 atcttctaca taaaccgcca gtctggctga aatctgttca gatgaatctc cacgtttttt
    22561 caggcgcgat tgcaatgttt ctttagagat cgtaataaag ataggacgga ctaccgtccc
    22621 gaaatactca tataaatgcc aaaaaccatt tgctgtcaaa acggcgtaac aatttcctac
    22681 tgtcagtttc tttttaattt ctgctttact ggaaccatat ttatgttgag catattggtc
    22741 ccactctgca aactcatttc ccttgatcat ctgattgaat gtattgtcag aaacaaaata
    22801 ataatcctct tgatcttttt ccccaggtct tggtgatcga ttcgtgtaag taatgatctt
    22861 ttgttccggc tgaaaaatag cttctgcgag tgaagttttt ccagaaccag aaggaccgat
    22921 aaatacatag caataattca ttgtcatttc tcctttgatg attgaacaag tgaaaaagct
    22981 ttggaaaata tttccaaagc ttttaaaagt aactttgctt ttttgtattt tataccagca
    23041 agccagtcac aatagcactt aaaaagctga ctaaagtcgc tgttagcaaa attttcaatc
    23101 cgtgttttgc gacaagattt cctttttctt catttaatcc cttcatcgca ccagaaatga
    23161 tcccgataga agagaagttg gcaaatgaaa cgaggaagac agatataatt gctgttgtac
    23221 gctcagtaaa atggaaatta ccatttgcta attctgtcat agcaacaaat tcatttgtaa
    23281 ccagtttagt cgccatgata ctaccggcat ccactgcttc tttccatggg attccgctga
    23341 tgaaggcaag aggggcaaag atgtaaccta ggatctcttg gaaagtgatg ccgaatatcc
    23401 cgtggaagat agcattgatc atggcaatca aagcaacaaa accgataagc atagcagcaa
    23461 ccgtgatcgc cacatggaaa ccatcaagaa tgtattctcc caacatttgg aaaaatgtct
    23521 gttctttgtt ttcttcaatg atcagctcat cttctttttc ttctaataca tagggattta
    23581 caattgaagc cagaatataa ccgccaaata agttcaaaac aagggcagtg atcacatatt
    23641 tagaatcgat cagtgccata taagatccga cgatcgacat agaaactgta gacatagccg
    23701 atacactcat ggtgaacaaa cgtttctcag ataggaaggg taattccttt tttatggaaa
    23761 taaaaacttc cgattgtcct aaaatagcag aagctactcc attataagat tccaatttgc
    23821 ccatcccgtt gattttactt aatcctagac caacagcttt cataaataat ggaagaatct
    23881 tgatatagcg tagaataccg ataatagcag aaataaatac aattggcatc aatacggaaa
    23941 gaaagaaagg tgcacttcct ttatttgcta caccggcaaa aacaaagtca acaccttgcc
    24001 cagcaaaagc aagaagatga tcaaacattt ttgccaatac agaaacaacc gctgtaccaa
    24061 aagttgtacg taataatacg aaaccaagaa caagctgtaa aacgaacatc acaattagtg
    24121 gtttcaattt gattgctttt ttgttactgc tgatcaaaaa agcaagccct aatgtgacaa
    24181 ataaaccaat aatacctaat aaataactca aataatcact cctattccaa attccgtttt
    24241 attttacctg ttttgcaaat aaaatactaa tctttagtga aattttcatt acttttttag
    24301 agtgaaacgc attcattttc cattagaaaa taaagcgaac aaaataaaag gtttaaaaag
    24361 tgtaacgtct tagtaaagtc
//
LOCUS       DANKEF010000036        24270 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000036 DANKEF010000000
VERSION     DANKEF010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.036"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..571
                     /locus_tag="PWZ48_001713"
     CDS             <1..571
                     /locus_tag="PWZ48_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2261370.1"
                     /translation="ASRIAIMSEGRVIQFDTPENILRHPANDFVEELIGEDRLLQAKP
                     NATRVGEVMLNTAITITPEKSLQEAIKLMREKRVDTLLVTDNSNVLKGFIDVETLNKK
                     RGKVSSVGDILNKHVFYVKESAYLRDTLQRILKRGLKYVPVVDEQNKVVGILTRASLV
                     DIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            575..1216
                     /locus_tag="PWZ48_001714"
     CDS             575..1216
                     /locus_tag="PWZ48_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261371.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            1213..2130
                     /locus_tag="PWZ48_001715"
     CDS             1213..2130
                     /locus_tag="PWZ48_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2261372.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            2127..2798
                     /locus_tag="PWZ48_001716"
     CDS             2127..2798
                     /locus_tag="PWZ48_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261373.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(3339..3827)
                     /locus_tag="PWZ48_001717"
     CDS             complement(3339..3827)
                     /locus_tag="PWZ48_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HDL2261374.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(4028..4294)
                     /locus_tag="PWZ48_001718"
     CDS             complement(4028..4294)
                     /locus_tag="PWZ48_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2261375.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(4294..4557)
                     /locus_tag="PWZ48_001719"
     CDS             complement(4294..4557)
                     /locus_tag="PWZ48_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2261376.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            5033..5218
                     /locus_tag="PWZ48_001720"
     CDS             5033..5218
                     /locus_tag="PWZ48_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2261377.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(5360..7183)
                     /locus_tag="PWZ48_001721"
     CDS             complement(5360..7183)
                     /locus_tag="PWZ48_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HDL2261378.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(7781..10486)
                     /locus_tag="PWZ48_001722"
     CDS             complement(7781..10486)
                     /locus_tag="PWZ48_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HDL2261379.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(10792..11739)
                     /locus_tag="PWZ48_001723"
     CDS             complement(10792..11739)
                     /locus_tag="PWZ48_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HDL2261380.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            11929..12198
                     /gene="rpsN"
                     /locus_tag="PWZ48_001724"
     CDS             11929..12198
                     /gene="rpsN"
                     /locus_tag="PWZ48_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDL2261381.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(12374..12736)
                     /locus_tag="PWZ48_001725"
     CDS             complement(12374..12736)
                     /locus_tag="PWZ48_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2261382.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(12747..13868)
                     /locus_tag="PWZ48_001726"
     CDS             complement(12747..13868)
                     /locus_tag="PWZ48_001726"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDL2261383.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(13884..14234)
                     /locus_tag="PWZ48_001727"
     CDS             complement(13884..14234)
                     /locus_tag="PWZ48_001727"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDL2261384.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(14364..15875)
                     /locus_tag="PWZ48_001728"
     CDS             complement(14364..15875)
                     /locus_tag="PWZ48_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDL2261385.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(16197..17549)
                     /locus_tag="PWZ48_001729"
     CDS             complement(16197..17549)
                     /locus_tag="PWZ48_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDL2261386.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLAATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(17702..18778)
                     /locus_tag="PWZ48_001730"
     CDS             complement(17702..18778)
                     /locus_tag="PWZ48_001730"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDL2261387.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            18974..20719
                     /locus_tag="PWZ48_001731"
     CDS             18974..20719
                     /locus_tag="PWZ48_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HDL2261388.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(20999..21436)
                     /locus_tag="PWZ48_001732"
     CDS             complement(20999..21436)
                     /locus_tag="PWZ48_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDL2261389.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(21420..22793)
                     /locus_tag="PWZ48_001733"
     CDS             complement(21420..22793)
                     /locus_tag="PWZ48_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261390.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(23115..23603)
                     /locus_tag="PWZ48_001734"
     CDS             complement(23115..23603)
                     /locus_tag="PWZ48_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2261391.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(23625..24227)
                     /locus_tag="PWZ48_001735"
     CDS             complement(23625..24227)
                     /locus_tag="PWZ48_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2261392.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         6959 a         5163 c         4488 g         7660 t
ORIGIN      
        1 agcaagccgt atcgccatca tgagtgaagg ccgtgtgatc caatttgata caccagaaaa
       61 tattttacgt catcctgcaa acgatttcgt agaagagttg attggagaag atcgcctact
      121 acaagccaag ccaaatgcga caagagtcgg cgaagttatg ctaaatacag caatcacgat
      181 tacaccggaa aaatcattac aagaagcaat caaactgatg agggaaaaac gtgtagatac
      241 gttgttagtt accgataatt cgaatgtatt aaaaggattt atcgatgtag aaacgttgaa
      301 taaaaaacga ggaaaagtat ccagtgtcgg cgacattttg aataaacatg ttttttacgt
      361 aaaagaatcc gcctatcttc gtgatacctt gcaacgcatt ttaaaacgcg ggctgaaata
      421 cgtgccggtc gttgatgaac agaacaaagt cgtaggtatc ctgactcgtg catcattagt
      481 ggatatcgtc tacgatgtga tttggggaga agatcccgca actactgatg ttgcagagtc
      541 agctactcct gaagaatcta aggaggtctg acctatgcag cagttttttc atcagcacgg
      601 ttcagaaatt ctctcaaaag gttttgaaca tctttatatt tcaggacttg ctttgatcat
      661 tggtatcatc attgctgtac cgcttggtgt cttactgaca cgttttccaa aagctgccac
      721 gattgtgatt ggcttaacca gtgccttgca gacaatccct tctcttgctt tattggcatt
      781 gatgatcccg ctatttggcg taggtaaatt acctgcgatt atcgcactct ttatttattc
      841 attattgccg attttacgta atacgtacat tgggatgaaa aatgtcgatt ggaattatcg
      901 ggatgtggca aaaggcatgg ggatgactga gttccaatcc attttttccg ttgaattgcc
      961 cattgctatg ccaacgatca tggctggtat tcgtttagct gccgtttacg tgattgcatg
     1021 ggctacactt gcctcttata tcggcgctgg tggtttaggc gatttgattt ttagtggact
     1081 gaataattac caaccagatc tcattttggc aggtacgatc cctgtaacaa ttttagcact
     1141 attggctgat tggcttctag gtctcttgga acaccggctg acaccgatcg cacttagaga
     1201 ggagaatgaa gaatgaaacg tatactaaaa atgaccatgg ttcttttctc tcttcttata
     1261 cttgcgagtt gttccttccc tggtcttgcc agcaatacag atgaagacac catttcgatc
     1321 acaggtggaa tcacttcaga agcacagatc ttagcaagtc tagttgccgg aatggttgaa
     1381 cattatacag ataaaaatac cgcaatcatc aacaatctgg ctactacaac tatcaaccat
     1441 caagcgatga tgaacggaga tgcttcaatt tcagcggctc gctacacagg aacagattta
     1501 acgactacat tgaaccttcc tcctgaaaaa gatccgaaaa aagcgtttgc taccgtgaaa
     1561 gatgaatttg agaaaagata cggtcaaacg tggttcccgt cctatggttt tgaaaatacg
     1621 tatgtctttc ttgttcgaaa agacaccgca caaaaatacc atctatctaa agtcagcgat
     1681 ctgaaaaatg tagcggatga attagtagct ggtgtagata cttcttggat caatcgtaaa
     1741 ggagacggtt atgacggctt ccaagaaact tatggcttct ccttcaattc gatattacct
     1801 atgcagattg gacttgttta tgacgcagtc gaagcaggaa aaatggatat tgttcttggc
     1861 tactctacgg atggacggat cgccagttat gatctagtca tgttggaaga tgatcgtcaa
     1921 ttcttcccac catatgatgc tgctccagtt gtagacaaca agatattagc tgatacgcct
     1981 catttaaaag aagccttaaa taagttaggt cataccatca gtacggaaaa aatgcaggaa
     2041 ttgaactacg aagcagataa taatctagtg gaaccatcag tagtagcaca acgtttctta
     2101 caagaaaacc actattttga agggaagtga agcaattgga taaaatgaat gtctttcaac
     2161 aatttttcta ttatttccag gaaaatggct cctatatctt tgcacaattt atccgtcact
     2221 tcctgatttc tatctatggt gtcctctttg cagcagttgt cgggattccg gttgggatca
     2281 tgatttctag gagacgtaaa ttagcaaact gggtgattcg tttggcgaat atcatccaga
     2341 cgatcccttc tctagccatg atctccattt taatcattgg attaggcctg ggagtcaatg
     2401 tcgtgattgt tactgtattt ttgtattccc tcttaccgat catcaagaat acgtataccg
     2461 ggatgatcca agtagacaaa aacatccttg atgtcggcaa aggcatgggg atgactgcta
     2521 gacaacgact attcatggta gagttaccac tctctgtttc tgttatcatg gcaggcataa
     2581 gaaacgcgtt agttgttgcc atcgggataa ccgcgatcgg tgcattcgtc ggtgctggtg
     2641 gtcttggaga tatcattatc cgaggaacca atgcaacaga gggcacatcg attattttag
     2701 caggtgcatt gccaactgca ttaatggcga tcatcacgga ctggctttta ggtattttgg
     2761 aaagacggct agatccagcc agccgtacat cacgctgatg ttattgtaaa aaacaaaagc
     2821 ttctgcgacc atttgttggt cgcaaaggct tttttagttc tgtcattttt aaggcaataa
     2881 tccactctga ttcttccctc cttatgggca aagagttgct tttcttagct atttatccat
     2941 tccgtatact ccaacttcat actgccaacg ggttcatatc tgcatccacc gactaagttt
     3001 ttttcttatc cgctaaggca ttttgccaat ccattttccc atcactcgtc atggaattca
     3061 taccacatga tctctaaata ctcgtctgct tgttctttga attgggcatc aattcttaca
     3121 tcctttggcg tggctttcat ttaagccttt tccatcagtc ctcgattgtt cgatgccttc
     3181 agctgcatca tcaaaactgg tgactgccgt atcggtaact tcgatcatct tttatacaat
     3241 cgggaacttg ccatcctcat ccttggctaa aaacatatct agccattcca gcttctcgcc
     3301 atgggaggtt tgcggttcct aaaaccggta cacgattctt atggactgca tccgtcgcaa
     3361 ctagacttgg cgatacgatg atccttcacc agaagagcac ccccgtaaac caattgatcg
     3421 atatattgcc aataagaaaa gacattggcg taaaagatat cgatccctcg tgatgcattg
     3481 ccgttgatac tgctattcat ggccgaaagc gccacgactt taacttcttc cgtttgagtg
     3541 acattcatgt tcggcaatgt cgctttggat acccgctctg ctaaaggaac gacactggta
     3601 ttgtatttcg tatctgtatc ttttccaaaa gaccatgcca acgaatcctc tgggaaccaa
     3661 taagacgact ccggttgatt gtccattgct tttttcaaca tacttttggc tgtttctcga
     3721 taaacgacat ttccaattgt cttcgcctca ctttggcgat aatcgatggt cgttgccact
     3781 gccaacacta acaacgatgc tccaatttta ctgattttcc ttgtcacact tcttcctcct
     3841 tgttttacat aaacccgtca acattccatt cctaaactca aacaatgacc cttcaaaggt
     3901 ggagatatag gaacaaatta aagaaatcat gcaataaatc caagaaggtt ggttttagaa
     3961 caaaaaaaga gtctcctttt tggggagact ctttgttaac tatctaaaaa ggttggctct
     4021 tctcacttca ctcataatgt gtccaggcag aataaatttc tacaacttta ttttcctcat
     4081 caactttgta aaccactcga tgctgtctat tcagcctgcg tgagtatctg ccttcatgtg
     4141 taggtctcag cttttcaaaa gactgcgtgg gcatatatgg atcttccttc aatgtctgaa
     4201 tcacttcttc aaattgtttc tttaaattcg tttgcttgat ttttcttaaa tccactttcg
     4261 cagagttttt gattgcgact gtatagttac tcatcaaaga gcttcccaat caatatcatc
     4321 gatattcgta aagctactgt cgtctttttc gcggtctcgt actttatcta aagtacctgt
     4381 ttgctcaaga aaaagggttt cttgaatcgc tctccagtct tccagcccaa tcaatacagc
     4441 attgtttcca ctacgatcac taataatctc gatttcagta tgattttcat ttacctcttt
     4501 caataactga taaaaatttt tacgagcagt gctaggattc atcgttgtat tagccattgt
     4561 ctctcctcct ttttctgata taattatagc gtacttgatt aagtacgtca aaaaaataca
     4621 ataaagcact taaattcagc aaacttttcc tcttttttac atagagaaaa agaccccttt
     4681 ttagggagga tctttgtcca cgctcacttc ttactccttt acgactagcg gtagccaaac
     4741 gtaaagtaat tggtaacaac gtatcatctt tagcaaaggt cgaatccaaa gcctgtgagt
     4801 ctatgcaaca acaccaatac acgaaggttc ctcgttgcag tgtgacttga ttgatatctt
     4861 gaataacctg aggatacgca cgaaagattt gtaacggtta ttcaaaactg attttggccg
     4921 tcaagtcttc tactgcctca aaaatgattg cctctttcgt ctcctgtcat tcatgttcac
     4981 tatcgatttg atacttgctt agaccgttgg gtttgtgaat caccaattaa aaatggcact
     5041 gagttatggt aacaaaaaat ggtcacttga cgaggctgag attcctactt atgaagacac
     5101 atggcactta agagtcgagg taaaggaaga agctgaaaat ataggaggct gtacattatt
     5161 tggcaaagga ttcggaaatt atgatcaaga catcaaaatc aaaatgtttt acagctaaat
     5221 aggaagaata ataacatttc atacgctgtt cccatcttta atggcttata gagggcacct
     5281 ttagctgaga ataagacaaa acagaccctc tagcttcaat taagagctag agggtctgtt
     5341 tttgatttta aaacttggtt tatttcttca aatggtagct ataggtgctg acaacgatat
     5401 tttcacgcca agacaatcgc aatcttaacc gcaaacttgt ttggttatgg agaatattct
     5461 gccagcgtct gtttggtaca aattgaggaa tcaacacagt agttgtatag ttttgtttcg
     5521 ctgcattttt acttacgata tctacataac ggataattgg attttcaatc gagcgataag
     5581 aagagtggac gattgaaaaa cggacatctg ggaaatgctt tttaaattct gcttcgattt
     5641 ctttttcttt ttcgacatct tcgtctagcg aaacatgcat cgcaacgaca taatcaccga
     5701 tcgaacgtgc gtaattcaac gcaccgatat ttacccgtgt cacattcccg actaatacaa
     5761 gaacggtatt cccgtcataa tcatgtagtt ttgcatcatt ttctaaacga agctgttctg
     5821 ctactttttt ataatgatca tggatcttat aaaaaatgaa cataacgatt ggcataatga
     5881 tgaagaaagg ccaaatgtcc cctaaccgat agacaaacaa aatcgcaatg attgcatatg
     5941 agatgaacgc acccgtaata ttggcaatcg atttgcttaa ccatcttttt ccttcttttt
     6001 tccatttgac gaccatccct gtttgagata aagcaaatgg aatgaacacg ccgatagagt
     6061 ataacggaat caagcgttct gttgagccat ggaaaataaa cagcaatacg atcgaaccta
     6121 gcgctagtgt gataatccca ttggaatagc ctagtcgatc cccacgatct tgatacatgt
     6181 gaggcatgaa tttatctttt gccagattgt atgcaagaac tggaaaagca gaaaaaccgg
     6241 tgttagcagc aactgctaaa atcagcgctg tagcaaattg aaggacgtaa tacaaaatac
     6301 catgtccaaa gacagcttta ccaatttgag acaaaacagt gacttctttt tcaggaacga
     6361 tcccatacca atagttgata aatgtgatcc ccacgaagaa gaaacctaag atcaatgcca
     6421 tcatcgtcaa tgtagcagcg gcattttttg ctcttggctt cttaaagaat ggtacagcat
     6481 tactaatcgc ctccacccca gttaaagagg aagaaccaga agaaaatgct cgtaaaatca
     6541 aagcgataga aacacccggc acagccgctc ctggaagtgc agtagcttgc aatggcacag
     6601 cacctgtcac gatattatat aaaccaacaa cgatcagtaa cgagatcaca aggataaatg
     6661 tgtacaccgg aaataatagg aacgatgcag attccctcag cccacgaaga ttcagcatca
     6721 tcagtagcaa aacgattgtc acagaaatag cgacttgatg accgtagagt gctggcaccg
     6781 ctgatgtgat cgcttcagca cctgctgaga cagatactgc tactgtcaac atgtagtcga
     6841 tcaataaaga gccaccggaa atcaagccag cattctttcc aagattctca ctgctgacga
     6901 catatgctcc cccgccatga ggataagcat ggatgatttg acgatatgat agcgttaatg
     6961 aaatcaataa aataataaca aaagcggcaa tgggtagcga ataccagatg gctgcagcag
     7021 acaaagcgac taaaacgaca acgatttgtt ccgtaccata tgcaatagat gataacgcat
     7081 cagacgacaa taatgccaaa gcagcaaatc gggttaattt gtgctcatca ttttcagcag
     7141 attttaaagg cttacctact aacaaccttt ttaagtaatc caatttctaa actcctttac
     7201 acacaatttt acttcaagtt ttatagtcga aaatttatag taaacaagat gttatagtac
     7261 gctcactgac cttaaaagtc aataaacttt aagaaaataa ttggaaaaaa acagaaaaat
     7321 atcaagcgct ttttcaaacg ctttaataca gcaaaaatct aacatttttt tatgggcttt
     7381 ttctttgaat tctataataa aaggatagaa cttgacttag aaaatcaagt tctatccttt
     7441 ttgatatctt aacttttgga agagagatgt aaggctgctt tctacccatc attcttacta
     7501 ctcgaagcat agcgcagttg tcacaacctc ttgataaacg gtgtttaaaa gcttgcatct
     7561 gcggtaataa gctcaaccaa attaaaaaca taagaagctg tttttatttg gtcgttctta
     7621 ttacttgatg cagagcagct tttttacaac ctcttggtac acggtgttca atcagctgac
     7681 cctcggtatt agctcaaaaa aacggcaaaa tatgaggaac tatttttcca tttttttctc
     7741 taatactcag gtcaaaacac tgatttcaca acctcttttt ttaatgaaaa atatggctct
     7801 catgcgccgc ttctttgaat ttcgtcattt tttcttcgat gaacggatag attgccgttc
     7861 ctacgattcc aaagacaaca aacaaaccga tcatgatcca gattgctttc gtagcatttg
     7921 gccaataaat tcctcctgca gattcccgta acaagtttac cgcatgagta aatggcaaga
     7981 acggattgat catttggaag aatttccctg agacttggat cggatagttc ccgcctccgc
     8041 cagagatcga cagtactaag atgataatag cgatcccttt accgacatta tcgaataatg
     8101 cagccaagac atagacgatc atcatgaacg ctaaggcgat caagacagca aatgacacgc
     8161 tgtagaccgg attttggaca tctactccta gtaagaaaat attccctaat gtaacgatca
     8221 agctttgcgc aatcgccatt gtcaaaaatg tcagcatacg agcaacgaat tgttctcgtt
     8281 tcgtaaaatt gtttcggtct ttctttccta aatagtattc tgtcgtagca acacttgaaa
     8341 gaagaacagc gccgacccac agacatagtg cagtataaaa cggggtgcta gctgaaccgt
     8401 tatttgcgat tggataaagc gcattggttt gtagctcaac aggttgtgta aagaagtcac
     8461 tttcttttgt tgcgtctagt ttcaataatt tgatgacttc gcctagatcg acttcttgtt
     8521 cgccttttcg gatcgcgtct gctgcttttt ggataccagc tttgatattt ggccagtcat
     8581 tgttgatcaa gtccgcagca gcgttcacag ctgtttcgac ttctggcatt ttttcattta
     8641 ctgttgtcaa tgtaccggtg atttcatttt ttagtgctgg ccaatcattt tggataaaat
     8701 cagcggccaa tcctaatttt tgctgaatga tcggtaattc attgttgtat aggtctgccc
     8761 cttcattgat gccattgacg atggtatcca tatttccatt cagcattgta ttggcatcat
     8821 ggatttcttg tccaattgca ggcatttctt tttgatattt ttctaaaatt gccactgcat
     8881 tcgttacagt tgctttcgtt gagctgagaa gcgaatcaaa gtcgatctgg tccgctttgc
     8941 tcagtgcgcc ttgtgcagtc ccaatcgtat cgatgacttg gtctagtaga gaagtgacag
     9001 tggaactgat ttgacctgta ttgataccag caatgatgcc attgatatta ttaacaacgg
     9061 catcaatttg atctagcaat tcatcaatag ccttcgtgtc ttcattttga acagctgtgt
     9121 tcaaagcaga taggcggttt tgcagattgc ttagcaaggg ttttaattga tttagctgat
     9181 caataattgg ctgtaatttc gtatttccag cacttgtttg caatttagtc aggaactcga
     9241 ccagtttatc gattgcattt tgttgttgag atattccgtc aataaagccg ttcaatacat
     9301 tgctgatgtt tttcttttca tcatcagtca gcttaccatc ttcaatagct gttcttacag
     9361 tatctattaa cgaacctgtc gttgttgcca cttgtccaat tgacttcaat gtaacatcta
     9421 cactgtttgt gatgcttggt atcgcatctt tcagctgaga ggcacctgat tttgtttggt
     9481 ttgctagatc acttgcctga ttttctaaat ttttcacatc tggcaagata tcttgtactt
     9541 gttggatgat cgtcaagcct tgttttgctt catcgattcc ttggttcatt gtctcttgga
     9601 tcgaagcaaa atcgccgtcg atctctgcta gttgcttacc ggcattttgg atctctggga
     9661 ttttttcttg caaatcaagg atgatttttg cttgatcttt caactcaggc attttgtcat
     9721 tcaatgcaac gactttttca cccatctcat ctactttagg gatgtaatcc acgaattcat
     9781 ttgctttatt cagtttttcc ttgatttcag gtagttgact ttgtaattcg aggacttgtt
     9841 ttgtgtagcc gtcgatcgtg tccaaatttt catctgttga taaaatcata tctttaactt
     9901 tattgatact tactaggttc gtatcgatgt cgtaacctat ctcattgaat actttcaata
     9961 aagtcgaact ggctgttttg ataaattcat ttgtgatttg cgattggatc gaacttgctc
    10021 ctttatcggt gattttcggt gcaatcgcat tgattttttc atttaccgta tattcgattt
    10081 ttggtttctt gatatcacca ctagtaaaac tcagcagatc ttctgagaaa tcctttggca
    10141 agtaaatacc tgcgtaatac tttccggaac ggactccgtc ttctaattgt tcttttgaat
    10201 caacgaattg ccatcctaat tgtttatttt tatgaagcga ttcgatgact tgctccccaa
    10261 tggccacttc tttaccttga aactctgctg gcttgtctgc actgtaaaca gcaattggca
    10321 gctctccagt atttccatat ggatcccata atgctttgat gttaaaccac gcatatagcg
    10381 aagggatgat catgatcgcg atgatcagaa gtgttgcaat cgggtttttg aaaatccgtt
    10441 tccagtcaag caaatataat cgaagcgtat ttttgattga ctgcatagtt ctcccccttc
    10501 ttcttttttc agctgaatta ctttaccatg tatttcatac ttttttcaat cggctttttt
    10561 caaaaaagtc gaatttttgg acatatcacg gcaattgtct cattgtaatc tcttacatat
    10621 tgagaaataa ttaaggatta cagcctttac tttatcatga aataaaaggg aaagataatt
    10681 ttatgccaaa gtttttatct attatacgta tacgcaaaaa agctaaaaca ttttttgtct
    10741 ttgcccctac aaaaatgttt cagctttttt cgttttttat tcctgtattt cttatttttc
    10801 gaatgacagg atgattttcc cagcaggagg atgtccttca actagctgat gtgcttggat
    10861 gacatgctta agtgaagcag acagattcat tgaaataaaa acatgaaagg ctcctttttg
    10921 gtaggctttg attatgccag caaaggcttc tgcatctaga tattcctttc ttggaacaaa
    10981 cgattcataa aagccttcct ttttttgccg caaatctaga tctgggaggt catttaatgc
    11041 aacatacctg ccgcctggtt tcatgatctg gatgccggtt tctcctttga tacttccttt
    11101 cgtcgcatcg attacaaggt ctgcctgatc agcaaattgc agacctggat ttgttttatc
    11161 ataagcagca aatgctgaag ctcctaattt ttttaccttt tcctcatttt tactggatgc
    11221 actggttaag attcggattc ccttttcatg gagaagctgg atcagacttg acccgaccgc
    11281 acctgatgct cctagtatca ttacagtatc tgtcggctgg atttccgtca aatggttgat
    11341 tagattataa gccgtaattc ctggcgtgac cattccggct gcttcttccc aactcatttt
    11401 atctggaatt tttgctactt ttgctgatgg caacacgact tcttctccgt aagtcccgcc
    11461 gactgcatgg acagctactc gatcacctac atgaacagtt gtgacatctg aaccaatctc
    11521 tgtcacaata ccagcgccat cattacctgg tacataaggg aatttcaatg ttcgtatctc
    11581 tttcatttct cctaaacgca aggcaacatc ataaggattc acactgaatg cttttatttc
    11641 tacacgaaca tgtccagaac cgacttctct gggatgagca tcgatttctt caaaaacatc
    11701 ttgtgcttct ccgtaaccgt taattgcaaa tcttcgcatg gtcaccatct ccctttttct
    11761 ttaagtttac gtttatgtct ttattttgac aaaaaaaatg tcttttcgtt ttggcgcatg
    11821 attcaaaaca aaatagttgt cttttttcgc gtaacgtgtt aaattattta tctaaaagcg
    11881 taaagattac gatttacgct aataaaaaat aattggagga atttgttgat ggctaaaaaa
    11941 tcaaagattg ccaaagcaaa aaaacagcaa gaaacgattg aacgctatgc tgctttacgc
    12001 caggaattaa aagcgcaaaa agattacgaa ggcttgcgta aacttccttt agatgctcgt
    12061 ccagaacgat tgaaaaatcg tgatttaatc gatggacgcc ctcgcggata catgcgtaaa
    12121 ttcgggatgt cccgtataaa attccgtgaa ttagcacata aaggtttgat tccaggagta
    12181 aaaaaagcca gctggtaatc caagtctggc tgtaatgtaa aggaggaggg cgcatggacc
    12241 actccttgga tcatcaaaaa agatacgttc gcttcttcta gcagaaatgc tggtactaga
    12301 agcagacgta tcttttttta cgcacagctg gtcctttttt atatgtaaag gctgcatggt
    12361 tacttcgcta tatttattta gtaacaggag tcagtgcttc gatacccaca ctaattttca
    12421 atgctcggtc aacttctgcc ataatgggtc gatccaaatg gcagaccttt tctttcaatc
    12481 ttgatttatc gatcgttctg atctgctcca acaggataac ggagtctctt tctattcctg
    12541 ttccagtagc tttgatccca atgtgagtcg gaagcttagg tttagccatt tttgccgtta
    12601 tggccgcaac aatgatcgtc gggctaaaat ggttacctag attattttga ataaccaaaa
    12661 ctgggcgtgt tccgccttgc tccgatccca cgactggaga aagatcagca aaataaatat
    12721 ctcctctttt taccatagga tcctccttat tcttgatatt ctcttggtat ccgctgtcct
    12781 aatccgcaag ccacttcata gtggatcgta cctaattgat ctgctatatc ctgcatagtg
    12841 atttctttgt ctgcattttt gccgataagc gtcacttttg ttccgacagg aaattcttga
    12901 ggcaatcgga tcatcagttg atccatacat acacgcccaa tcgtttcaca gtagttccct
    12961 tctaccaata gagaaaaacc ctgcatcttt cgtggccagc catctgcata accgatcggt
    13021 atcgttccga tccactctgc ttcaggagtg atatatgttt ctccatagcc gattccttca
    13081 cctgctggga gttttttgac ttgaatcaat tcagaaacca gctcaagtgc tggctgcaat
    13141 ggataaactt ccggtagtgc gtgcccagag ggattaagtc cgtacattgc tactccataa
    13201 cgaatcatat tgccgattgt ttcatcatgc cataaagccg ttgcactatt gctgacatgt
    13261 acatatcgag gaagttcggg aagttttttc agcacttcta taaagcgttc tttttgcaga
    13321 ttccaatatg tatcatctgc ctgatctgct gtagaaaaat gggtaaataa accttcccat
    13381 tctaatacac gactttcttt gatgaatgca gctgcctctt ttacttcttc aggtgtacaa
    13441 aatccgatac gacccatccc agtgtctgct tttagatgaa tcgaaagagg cgtctctagc
    13501 ttacttttct ctaaaacagc agctgctgct tgcaaccatt cccgtgtacc tgcagttact
    13561 gataaatcat gagcaacagc tacagtcagt gcgtcgtaag gaaccatatt caagataagg
    13621 atgggctgag taaatccagc ttctctcaac tcgactcctt catctaaatt tgaaacacaa
    13681 aagcccgaag ctccacctgc aactgctgcc tcagctgttt caatagctcc atgaccgtat
    13741 ccgttcgctt ttactactgc aaacaattct tttccttgcg gaagtcttgc aacttcattt
    13801 gctacatttt cagttatagc ttttttatgt atcactgctt tcgttgggcg gtgccaagcg
    13861 acaaccatcg tttttccttc tttctagtct tccaaaataa tttgtgcaaa agctgtttca
    13921 tttgtatgag taatactgac ccatacattg ccttcatgag gtgaacgcgt cacaacaggc
    13981 tgaccatttt cattttttaa aatctcgaca tcttgaaagg taacttttcc gatcccagtt
    14041 ccccaagctt tagagaaagc ctctttgcaa gcgtaacgcc cgcctaaaaa ttctacttgg
    14101 cgatgaaaag gcaaggactg gaatagtttc atttcatcag aggtcaagat tcgtgcaatg
    14161 aattttggtt tttcttcgat cagccgagtg atcctaggaa gttcgactgc atcgatccca
    14221 attcctttta tcatatgctt ttctcttttc aataaattta gaacactctc attctaccaa
    14281 attataacca ttttgccaca aataagtcca caaataacaa aaatgaaagt ttctcttaaa
    14341 aaagagaaac tttcatttta tgtttaatcg ttatttgaac ggattacaaa tccgcgtttt
    14401 ttatcattgt gacgtttacc gccatctttt tggctgttat tatttttgct ataaccgcct
    14461 tttgatttgt ttccacgata attaccgccg tcacgacgat tacggttgtt tccgccacca
    14521 cgtttgccat ttttattgta tccttttttc tgcattggca atgggcgttc tggtgtgatc
    14581 ttcactggca ctgcatcagc tggatctttt gctgtcgttt ttagcaaaag tgcaactaaa
    14641 tcttgtgcag aatattcttc taataattta tctgctgttt gcaggtattt atctagacca
    14701 ttttctgcaa gttttgtttc gatttgttca actgcagcac ctaattgacc tttgaatgct
    14761 tctttttcgg ttggtggacg taaagtcgtc atccgttttt tcgtcaagtt ttcaatcaca
    14821 tgcaagtagt ccatttcatt tggtgtaacg aaagtcacag acataccgcc ttttccggca
    14881 cggcctgtac gtccgatacg gtgaacgtag ctttctggat cttgagggat atcatagttg
    14941 tacacatgag tgacacctga aatatctaat ccacgtgcag caacgtctgt cgctacaagg
    15001 atatctaaat gaccgctctt aaatgaacgc aatacgctca ttcgtttttg ttgagacaaa
    15061 tcgccatgga ttccttctgc acggtaaccg cgtgcttcta atccgcgtgc caattcgtct
    15121 acacggcgtt ttgtacgtcc gaagacgatt gttaattcag gtgtttgaac atcaaacaag
    15181 cgagtcatga tatcgaattt ttcgtattct tttgcgcgga catagtattg atcgatcaag
    15241 tcagctgtca tttctttcgc tttgatcttc acatgatgtg gatttttcat gaatttaacg
    15301 ccaatattct tgatagctgg cggcatcgtt gctgagaaaa gtaatgtttg tctttgatca
    15361 ggaacttgtg aaatgatttt ttcgatatct tccaagaagc ccatattcaa catttcatct
    15421 gcttcatcca aaactaatgt ttgaacggtt ccaagtttca atgtgtggcg gttaatgtgg
    15481 tcaagcatac gtcctggtgt acccacaacg atatgtggac gatctttcaa gccacggatc
    15541 tgacggccaa tatctgctcc gccgtaaacg gcttgtacac gtactttttt gtcgcgccct
    15601 aaacgatata attcttcttg tgtttggata gctaattcac gtgttggtgc gataactaaa
    15661 ccttgtaatt cgtgacgatc tggatcgatt ttttctaaca ttggtaatcc aaatgcagct
    15721 gttttccctg tacctgtctg tgcttgtcca ataacgtctt ttccagctaa tgctagtgga
    15781 atcgtttctg cttggatagg cgttgcttct tcaaaaccag cgcgttcgac tgatttcaac
    15841 aattctggtg ataattctag ttctttaaat ttcaaatgaa tcctcctata tggttgttaa
    15901 ctgaggatgt agcttttttg ctaccccgtc gatttgttcg gtcaaagtgt attgtttctt
    15961 tttgacagtg gccacgcacg tttttatcca ttcttagccc ctgtggcacc accgaaaaat
    16021 cactttgcga agtttaggca ttagaaaagc caaacgggtc gcgttcagct ttatcattat
    16081 tatgacatct ggcttcaaca agttgtttct atggcaaaac cagtccaatt tctcttcatc
    16141 tttcggcaat aggttattct aacatagttt ttcgttttct gcaagtttac agatttttat
    16201 ttcatttgct gaagttgttc taccacttca aataagccca tgctgttgct gccttttagc
    16261 ataatactat ctgttgctgc aagattttgt ttgatagcat caatcaattt attcttttct
    16321 ttttcaaaat agaacacagg tagttggggg tatttttggt tcaattcttc ttttagtgca
    16381 tgcatttgtt ccccataaag gaaaatcatg gcgaatttct caggatcgat atgttcactc
    16441 atttgcgcat gcatatttaa agactctttc cctaattcca gcatatctgc taacacagcg
    16501 attctgcgtc cttttgttgg cagattttta aaagtatcta ataccagtcc catggctgtt
    16561 ggatttgcat tgtagacatc gctcaaaata gctgcaccat ttcctgccgt tagccattcg
    16621 gttctgtttt gagtgataga aacatgggcg agcccttggc gtatctcagg tatggacagt
    16681 ccaaagaagc gcccgatcgt atacgcaatc aaagcattcg ttacattata acttccaggc
    16741 agcgggatcg taaatgtttc tccttctact acaaaatcgg tttgtgcttt tccttttgct
    16801 gtcacttccg catgaacatc gccatttcca ataccgaatg ttgtgactgt ttgtactaat
    16861 gttgccgtta aaggtgtcag caacggttca tctgcgggca caacgagtaa tccgtctgat
    16921 acaagtccgt cagtgatttc cattttggct ttagcgattc cttctcgcga cccaagattt
    16981 tctatgtgtg cttcaccgat cattgtgatt gctgcagctt ttggttgagc cataagtgaa
    17041 agttccgtga tttcattggc gtgatccatg cccatttcca aaacgatctt ttctgtatct
    17101 tggggcatgt gcaaaatcgt gtaaggaaga ccaatattat tgttgtaatt tccttgcgtc
    17161 ttatatgttt tgtatttttc agacaagaca gacgcggtta gatctttcgt ggtggtttta
    17221 ccattgcttc ctgtaattgc aacgacttct gcgcctactt tttccaagta ataggttgcc
    17281 aattgctgca tggctttcaa aggatctgct acaggaataa ctggaaaatt ttttggagct
    17341 tgttcaacag gcaagctcca aaatgaagca gatgctccgt ttgtgatcgc ttgctggata
    17401 aattcatgtc catctcgcgt accagcaaga ggaacgaata aacttccttc ggttatcttc
    17461 cggctgtcaa attcgacatt ggtaatcttt gtcagtgcag agcattcgct gcccacggct
    17521 tgtgcaattt cttgtattgt tagattcatt gagatctctc ctaaaaaagg ttgtgaaaaa
    17581 agcgtttggc ctaaacggcg ggttcgcatg actctcactt ccaagtaaat ttctctattc
    17641 gtatttagaa gctagaatga acgcggttac gcaatcactc cagcctcttc tttatttctc
    17701 tttattcatt tttttcaaaa aaagattgac gctgatggta tcgattcatt cctaactgga
    17761 tcagttcttc aatcaaatca ccgtatttca agcccatatt ttcccataag agtgggtaca
    17821 tactgaactc cgtaaatcct ggcatagagt ttaattcatt caggaataat tcatttttat
    17881 ttgtcaaaaa gaaatcgcac cggctcaatc cgcttccacc taacatcgtg taagctaact
    17941 tcgcgtactc ttgcgctttt tgataaactt cttctggcac ttcggctgga atctgcattt
    18001 cgattttatt attgatatat tttgcttcat aatcatagaa tgctacgtct tttacgactt
    18061 caccaggcaa agtcgtccga acgtcttcat ttcctaatac agcaacttcg atttcgcgcg
    18121 cttcaattcc ttgttcaacg attgctcgag aatcatactg ataggctgtt gctaaagcat
    18181 tttgcagctc ttctcggttt tctgcttttg taatgccgac actagaaccc atattcgccg
    18241 gtttgacaaa catcggataa agcaaagaac cttcacattg atcaaatact tttttaggat
    18301 tttctttcca ttgattctta agtactggta cataaggaac ttgcggcaca ccagcagctt
    18361 gtaaaatata cttggtcatg attttatcca tggcacatgc actggtcaat acgcctgcgc
    18421 cgacataagg catattcaat gtctctaaga agccttggat cgttccatct tccccgtttg
    18481 gcccatgtaa aactggaaaa acgatggctc cttcttcttt gatttcgccc ggattgatca
    18541 cttttcctgt aaagccttcc tctgtctgtc cacttgggtc ccatgaaaga tgcaagacat
    18601 ctttgctggc aggtttttct gttagtaatg gacctttgac ccattgtcct tcttttgtaa
    18661 taaatacgag ttgaacttgg taataattat aataaatggc atttaaaact gaaaatgcgg
    18721 aaagaatgga cacttcatgc tctgcgctgc gtccgccata tagtaaagta atcttcaaaa
    18781 gattgtcctc ctgtattcta aatcgttcag tgatttactc tcatatttta gcacaaaaca
    18841 aaaataaacg aataacttat ttataaaaaa acgagccagc ttctaaaaga acgagcgtca
    18901 tttatttttt gtcagctaga ttatttgtcc cgttttttat aaaaagttat gataaagaaa
    18961 aggagggatg atgatggcgc aaacaaatcc acgcagccgc ttggcagaaa tcattcgagt
    19021 gttcatcaat tacaatgtag tgccaaactt tgtacagcaa aaaaatcccg aacaggtaaa
    19081 aaaagccttt gaagaactag gccccacctt catcaagata ggacagatgc tttctgttcg
    19141 agaagatttg ctttcttctg ccttcactca aacattcaaa acattacaag atagtgtacc
    19201 tagcgacaca ttttcaacgg tcaaaaagac gatcgaaaca gaactttctc tttcattatc
    19261 agatatattc gatgattttt caaaatcgcc ttttgcctct gcttctatgg ggcaggctca
    19321 tcgtgccaag ctgaagaacg gtgacgcggt agtcgttaag atccaacacc ctaacatcgc
    19381 cgaagagatc cgacttgatc ttcaactatt tgaacgagcg atccctttga tcaaatacat
    19441 tcctgaaact agcgtagttg atctaaaagg ggtactccaa gaagtaaaac gttccttgat
    19501 caatgaaatg gacttcttaa aagaaagtca aaatggcgaa caattctatc aaaagaacaa
    19561 tggctggaaa gagatccgca gccccaagat ttatgacgct ttttgttcga aaaaagtgat
    19621 cgtgatggaa gaaatgtctg gtaaaaactt gaatcattta atgaacgccg aaaacaaaac
    19681 tgaaactttt atcactggaa ttcaaaacaa gcagctgaaa caagaagttg caaagctttt
    19741 agttgagaac tttatgaaac aggtgtttga tgatggtttt ttccatgctg atcctcatcc
    19801 agggaatctg ctattccatg tactaacaaa agaagaacag acacaagcta gccgaaaaac
    19861 tgaaacagtc catgaaaaag agttcggttc ctttgctttt cgagcctcca cttctgcaga
    19921 agaccctgtt gcgccatata ccatcaacta catcgatttt ggtatgatgg ggcatctttc
    19981 tgcaggactt aggcaaaaac tcacacaagc cgttttagcg ctgtatacga aagatgctta
    20041 tcgaatcgaa aaagcggtcc ttcgactttg tcagcaagaa ggctcgtttg atgaaagtcg
    20101 gttccaccaa gaactgacaa gttttcttga acagtattat gattctccta ttgatgaaat
    20161 caacttgcaa gaagtattca cacatgtcgt gacgatttgt catcaaaata atctgcagtt
    20221 cgatcgtgat atcacgcttt tgctgaaagc atttgggaca ttagaagggg tgatccgcgt
    20281 cctcgatcct gaagtttccc tcatggaagt cgcttcgcct tttgctcaac attattttct
    20341 cactcatctg gatgtagaag atacattgaa acaaagcgga ttagacttat tggaagggat
    20401 gaaggctgcg cctaagatcc ctcaacagct ccaccatcta ttagaaatgt ggacttcggg
    20461 acaaggaaaa gtcaatttgg aattgaaaaa gcaagacaaa ttattgagca ggatcgaaag
    20521 tatgatcaat cggcttgttt tcggtatgat tttagctgca ttgatcgtag ggtcctccct
    20581 gcttgtccaa gcagcacctg tagaaaacgc agaggtcgtt tccttattag ggatatttac
    20641 gtatgctatt gccgcattcg tcattatttt tttggcaatc gatgcattga tccaaatgta
    20701 taagaagagg aaaaagtaga aaaaggttgt gacagaaaga atcagcttca agaaataaga
    20761 aggggtttta cataaattgt ttctcaaatt tttgtgaaaa ttcggcttat ttttcgaaga
    20821 ggctacttct gaaacaccga gaataaaaaa tgtgaaaaag ctgttctgca cccagtaata
    20881 agaacaatca aaaagggaac atggaacaca tttatcctgt ttccctgttc cctttttgat
    20941 gtattcgagc ataaataaaa taccttcgtt acctactatc ttctgtactt ggtaaacctt
    21001 agtcaattgc cttttttaac gcttgacgaa aagacatatg atctttctcc gtaaagggtt
    21061 ttggaccctt tgtccttatg ccagcttttc ttaactttcc actatgataa cgttgttcta
    21121 gaagcgtgcc gatattcgct tcgtcaaatt cttttccaga gtgatggaac actcgcacac
    21181 ccttcggcaa tacaagtgaa gctaaaccat aatcttgcgt gatcaaaaag tcattcttct
    21241 taaccatacc gacgatttta taatccgcac tatcagctcc gcggtctacg tagacgattt
    21301 ggacatgatt agcgtattct ttggtcgtat aatggtccac actggtcacg ataaccacgt
    21361 caagaccata ggattctcct aattttatga catcttcttt tactggagaa ccatctccat
    21421 caatgaatag cctcattctt tttcgccttc ttccataaat gataaacagt tactaatccg
    21481 ctcagtaaaa gaccaacatt gatccatttt gccaagataa gcagttgtac actttctatc
    21541 ggcactgatt catggttcag cgtgatcaat aatagatgga ttggctctcc catttctttt
    21601 gctaaaacaa acaaaagcgt catcaataaa aactggtgga tcacactccc tttattttga
    21661 gtagtatact tgacaacgat ccggcgatcc attggaatag ctgtatcagt ttgataatat
    21721 tccttattta gccagctgcc tgttactacg tgccagaaac taagcagaaa gacacctatc
    21781 gcaaaaccat ttggaatcaa gaggaccaat aaagaagaac tcagtccaag caattgcata
    21841 gccaatggag accatgttaa acgtttcaaa atccatggtc cgactatttt tgctgcaatc
    21901 aagcctgctg cataaggcaa atacataaaa atcgaaagtt tttctaatcc gtacagtatg
    21961 ccaacataaa aactgctaaa taaaaaaagg aaattcccta gcattccatt tagaaaagta
    22021 aacagattta gccagcttgg caacttccat tccttcttct ggtgtcctaa aacaaacacg
    22081 aacaagatga acagcagcaa gaaaccataa atagcaagat caaaaagcct ttcatcgaat
    22141 aacaatcggg catttctcaa aagaaacaat aagccaaaga atatcaaaaa taaaacaagc
    22201 tctcgcgtag ctaccagatt tcttttgatg tctttgaaat ccagttcgta acgcggataa
    22261 tgactaaccg tatggtaggc catgacgtaa aaaagtgtat aaatagtaaa gcttgctgca
    22321 atctgagtcg gtgcaggcaa ctcaatcgct tgatacagga gaaatagcca gatagctgca
    22381 aataagtact ttttcccatc catattgata aaaccttgcg tcttttcgtg aaaattgacc
    22441 gtggtattag caggtacaag ccatgctgca ctcagcccta gcaaaacact tgaaaaataa
    22501 aataaaggaa aataaaatac gcctaatatt ccaagcaatg aaccagcacc accaatcagc
    22561 agagcgatca tcagcaacgt gtaactatct agaccaaatc gtagactgtt gactaaaaat
    22621 agtccagtca tccgaaaggc ataaaacagc gtaaatggca aaatcgtcag ccaattatgc
    22681 gagtctgtta gtcccaaaaa taaaagataa ggaataaaaa taaccgtgtt acttaagata
    22741 aagggcgttg tttgtgtaaa attacgccca aatcgtttga gtttttcgaa catgacaatg
    22801 gctccttaaa cagtcttata actaatctgc tcttcgattt tagcattaaa ggggaaataa
    22861 accaatttta cagaatatat tcttgtaaaa aaaaatacag aaagaagcat aagcttcttt
    22921 ctgcattttt acggtacttg attactcaag cagacaagcc cttctccaca aaagtcttgc
    22981 ccgacacccc tgcatccggc aggtgataaa gatagaaatc aattatcctg tgcctccaaa
    23041 atcaattagg agctctgtaa accagagtcc ttctcgaccc gtcgcataaa tttactttaa
    23101 cacagttcct atgtttatgc gagagctttt ttcattttat aatcgaccat ttgaaatcct
    23161 tgaccgatat ctgaaataag ttcttctgta atctcaaatc cttcatgctt ataaacctcg
    23221 attgcttgat gattcccctg attcactcgc aaaaagatct ctctttcctt tgtttgcgca
    23281 gcaagttctt cataccagcg aaaaactgaa gaagtcaatc cttgaccacg tactttattt
    23341 agcaaatata atttacttaa ataaattttt ccatttatct cttcataagc agtgtaaccg
    23401 acagtctttt cttcgctttt taataaaaag taggacacac cttctgtcag ttctttttga
    23461 atctgttttt ttgactgata agttttcagc atgtaatcca cttgtttttg accaatgatc
    23521 ggcacaaaag cttccggcca tacttcttgt atgactgcca tcagctcatc cagttcattt
    23581 tctgttactt ttattttttc catacaccca cgtccttttt tacattataa atgaccttgc
    23641 gcttgatgga ataaaaagag catgatcatg ctatacacac ccaccgacca tggtttcaag
    23701 aaaatcacaa tctgcataaa ttccctaaaa gataatttcg tcaaaccagc tactagacag
    23761 atcaagtcgt ctggcgcaaa gggaagcagc aaggtaacaa ctagtacttt tttaatatct
    23821 ttcgtttttc ttgtcagcaa ttcatcaaat ttctgatact tttttggcga taaaataagc
    23881 tggacaaagg ctacgccata gtaacggaca agcaaaaatc cgattgcttc cccaatcact
    23941 aggccaatac aactatataa aagcccaaat ccattgccaa acatcagcat gcccgctact
    24001 gaggagatcc ctcccggcaa aataggaatg atcacttgga tcacttgcaa cagaacaaat
    24061 atcatcactg catacttgcc aaattgctgg ataaacgctt gcaaagacgt gctcgatcga
    24121 aaaatcccat gatgataagc ataaagaatc aacacaaaga aaaaaaacag accaataatg
    24181 ggcatgatct gaagacattt ttgtaaaact gatccttttt tagacatcta tattccccac
    24241 atcttttttc atttattaaa actgcctcct
//
LOCUS       DANKEF010000037        23828 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000037 DANKEF010000000
VERSION     DANKEF010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.037"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(53..787)
                     /locus_tag="PWZ48_001736"
     CDS             complement(53..787)
                     /locus_tag="PWZ48_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HDL2261393.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="PWZ48_001737"
     CDS             1114..1524
                     /locus_tag="PWZ48_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDL2261394.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="PWZ48_001738"
     CDS             1517..2218
                     /locus_tag="PWZ48_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HDL2261395.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="PWZ48_001739"
     CDS             2218..3804
                     /locus_tag="PWZ48_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261396.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="PWZ48_001740"
     CDS             3823..4044
                     /locus_tag="PWZ48_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HDL2261397.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="PWZ48_001741"
     CDS             4041..4802
                     /locus_tag="PWZ48_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HDL2261398.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="PWZ48_001742"
     CDS             5182..5691
                     /locus_tag="PWZ48_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HDL2261399.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="PWZ48_001743"
     CDS             complement(5766..6305)
                     /locus_tag="PWZ48_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HDL2261400.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PWZ48_001744"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="PWZ48_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDL2261401.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="PWZ48_001745"
     CDS             7318..8169
                     /locus_tag="PWZ48_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2261402.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="PWZ48_001746"
     CDS             8197..8832
                     /locus_tag="PWZ48_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HDL2261403.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="PWZ48_001747"
     CDS             8852..9625
                     /locus_tag="PWZ48_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2261404.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="PWZ48_001748"
     CDS             10033..10830
                     /locus_tag="PWZ48_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HDL2261405.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="PWZ48_001749"
     CDS             10808..11221
                     /locus_tag="PWZ48_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HDL2261406.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="PWZ48_001750"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="PWZ48_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDL2261407.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="PWZ48_001751"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="PWZ48_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDL2261408.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="PWZ48_001752"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="PWZ48_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HDL2261409.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PWZ48_001753"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="PWZ48_001753"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HDL2261410.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="PWZ48_001754"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="PWZ48_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HDL2261411.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="PWZ48_001755"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="PWZ48_001755"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDL2261412.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="PWZ48_001756"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="PWZ48_001756"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDL2261413.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="PWZ48_001757"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="PWZ48_001757"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDL2261414.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="PWZ48_001758"
     CDS             21905..22483
                     /locus_tag="PWZ48_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2261415.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="PWZ48_001759"
     CDS             22535..23017
                     /locus_tag="PWZ48_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HDL2261416.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="PWZ48_001760"
     CDS             23134..23508
                     /locus_tag="PWZ48_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261417.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6646 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagttt
//
LOCUS       DANKEF010000038        23653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000038 DANKEF010000000
VERSION     DANKEF010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.038"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..506)
                     /locus_tag="PWZ48_001761"
     CDS             complement(<1..506)
                     /locus_tag="PWZ48_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2261418.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="PWZ48_001762"
     CDS             779..3250
                     /locus_tag="PWZ48_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2261419.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="PWZ48_001763"
     CDS             complement(3353..5089)
                     /locus_tag="PWZ48_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HDL2261420.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="PWZ48_001764"
     CDS             complement(5086..5790)
                     /locus_tag="PWZ48_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2261421.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="PWZ48_001765"
     CDS             5921..6424
                     /locus_tag="PWZ48_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HDL2261422.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="PWZ48_001766"
     CDS             6384..6722
                     /locus_tag="PWZ48_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HDL2261423.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="PWZ48_001767"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="PWZ48_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDL2261424.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="PWZ48_001768"
     CDS             8273..8851
                     /locus_tag="PWZ48_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDL2261425.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="PWZ48_001769"
     CDS             8855..9376
                     /locus_tag="PWZ48_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2261426.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="PWZ48_001770"
     CDS             complement(9455..10009)
                     /locus_tag="PWZ48_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HDL2261427.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="PWZ48_001771"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="PWZ48_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDL2261428.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="PWZ48_001772"
     CDS             10549..10800
                     /locus_tag="PWZ48_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HDL2261429.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="PWZ48_001773"
     CDS             10925..11716
                     /locus_tag="PWZ48_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261430.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="PWZ48_001774"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="PWZ48_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDL2261431.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="PWZ48_001775"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="PWZ48_001775"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDL2261432.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="PWZ48_001776"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="PWZ48_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDL2261433.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            14014..14676
                     /locus_tag="PWZ48_001777"
     CDS             14014..14676
                     /locus_tag="PWZ48_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261434.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            14755..17982
                     /gene="fss3"
                     /locus_tag="PWZ48_001778"
     CDS             14755..17982
                     /gene="fss3"
                     /locus_tag="PWZ48_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HDL2261435.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQLVITPNANSKESGELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTIT
                     EKSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSL
                     AGNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTF
                     KPVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTL
                     FTAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGF
                     NDLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     EATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYE
                     NKKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDT
                     SKYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVP
                     SDSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            18096..18410
                     /locus_tag="PWZ48_001779"
     CDS             18096..18410
                     /locus_tag="PWZ48_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2261436.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            18423..18797
                     /locus_tag="PWZ48_001780"
     CDS             18423..18797
                     /locus_tag="PWZ48_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2261437.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            18807..19151
                     /locus_tag="PWZ48_001781"
     CDS             18807..19151
                     /locus_tag="PWZ48_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2261438.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            19222..20574
                     /locus_tag="PWZ48_001782"
     CDS             19222..20574
                     /locus_tag="PWZ48_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HDL2261439.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            20634..21776
                     /locus_tag="PWZ48_001783"
     CDS             20634..21776
                     /locus_tag="PWZ48_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDL2261440.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            21801..22055
                     /locus_tag="PWZ48_001784"
     CDS             21801..22055
                     /locus_tag="PWZ48_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261441.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            22311..23495
                     /locus_tag="PWZ48_001785"
     CDS             22311..23495
                     /locus_tag="PWZ48_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2261442.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            23492..23629
                     /locus_tag="PWZ48_001786"
     CDS             23492..23629
                     /locus_tag="PWZ48_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HDL2261443.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
BASE COUNT         7832 a         4218 c         5073 g         6530 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 ctggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tcagaagata aacacggaaa acttccttgg aaatctgaaa tttgtgaaaa
    13681 gtccttgtat atcaagggct ttttcttttt tctctatcac agttttaagc catttttggc
    13741 tgttttggac catttttcaa tcatattccc ttaaaaattg tcctagattg tccgacattg
    13801 actatcagtg tcgtcaaaat agggcaaaat ttagtagtca gcttgacgac aaatataata
    13861 cgaaaaatac caaaacattt accgaattta gcaacttttg aacccaagtt tctcaaaggg
    13921 tcagctatta gcaaaaaaga caaaacatgt acttttctac taaaaagttg atgtgatagg
    13981 cttctatcta aataactatt aggagttttt atcgtgaagc aaaatgatgg acgtatcatt
    14041 tggaaaaatc aaagtgagtt aaaactgatt ttaacaatta atgaattcat cgaaaaacac
    14101 ggaattacat ctagccgtca ataccagaaa aagctatcag aaaatccaaa ttctgcacca
    14161 agtatgtggt tcattaacaa aaaatatgga tcatgggaga atttattgat tagtatagga
    14221 cgagaaaata ctgggtatgg aaaatgggct agaatgtcag aacaagaatt attggaaata
    14281 gtagaagcct ttattaagtg cgagaagata acctctcaac gaatgtacga acaaaaatca
    14341 gtagggaaga atattccttc acttagtacc attaaaaaga tgttaggaga tatacgtcca
    14401 ctttttaaag aaaaaaatga tggatctcga tttactgatt tcgaactgct gttagaacta
    14461 aaaaatgaaa taatccgatt aaaactacag gatgatttat caatgacaaa gtttcgaaaa
    14521 ctagttcaat ccccaaaact accatcagtt gatacaatta tgaaaagaac gaacaaaaat
    14581 tgggaggaat tgatggcaga gattggattt gattatcgaa gaatcaaaat ttataagcag
    14641 agaaacaacc tatccaagac aaagaaaact aaatagtatc actctaaatc agacgttcaa
    14701 aattaaaatt tggacgtctt ttttattacc caaaaacgaa aggagattta ttttatgaaa
    14761 aagccaagat ttaagaattg gcgtttgctc gcaacactga gtctattatg ccaaacaatt
    14821 ggtggatcgc ttggcccaac gattgccttt gctgatgaaa ttactcatcc acaaacggta
    14881 actgtggaat tggatttggc tcaccagtat gctgtcgaag gtacatttag cgatggtcgt
    14941 cccatgtcag aagttaccgt tccgcactat gcagtttaca atggtgtgaa gcaagatatt
    15001 ttctgtattg agccaggggt gccgatttac aatgagttca ctcctggtta tgagaaaaat
    15061 ccattgcctg atatgtcgga gaaagcgaaa ttagtttccg ttttatggaa ggaagctggt
    15121 acggatgtgg atacacatat tgtggcccag aagatgattt ggcaagaagt taatggctac
    15181 acgcttcact caattaaacg ttctgatggt agtgcagtaa atatcgcagc cattgagtca
    15241 aaaatcaatc aagccattgc ggattatcaa aagaaaccaa gcttccataa ctccacagtg
    15301 aaaaccgtgt taggtcaatc gactactgtg acagatacga acggcttgaa tttatcggag
    15361 tttgatgaag ttgtcgagaa tatcgcaaat attgattatc gtgtgaacgg caatcaacta
    15421 gtgattactc caaatgccaa ttcaaaagag agtggtgaat taaccctaaa gaagtcagct
    15481 aatacaggaa caccggtagc ttataaaatg gctggtcaac aaactttgat ggcgggggca
    15541 attgataagc ccaataccta cacaatcaaa attgatgtgg aaactgaggg ttctttgaag
    15601 attaaaaaag tcgataaaga aacaggaaat gttgtaccgg gaaccgtttt ccatttagac
    15661 tttggaaaaa cattacctgc aaaagacgtg accaccgata aagaaggcat tgcgacattg
    15721 gatgagattc ctcatggcgc gaaagtaacc attactgaaa aatcagtgcc agctccttat
    15781 acgattgaca ctacaccaat caccgcaacc attaaatctg gtgagactat ttctgtgact
    15841 tcaaaaaata ctcgcgaaaa aggtcaaatc attttgaata aaatgggggt ggaaacagga
    15901 actgatcttt ggaacgacaa atactcatta gccggaaata cgtttgccat tcgcaaagac
    15961 agtccaaccg gtgaaattgt ccaagaaatg accacggatg aaaaaggtca tgcggaaaca
    16021 ccaaaagaga ttgccaatgc gttggaattg ggaacctatt atgtgaccga aaccaaagct
    16081 agtaatggct ttgtgaatac ctttaaacca gtaaaagtcg aattgaacta tgtcaatcaa
    16141 accgtggctc ttgttaccag taacgtaaaa gggcaaaacc aagaaatcac tggtgaaacc
    16201 actttgacaa aagaagacaa agataccggt aatgaaactc aaggtaaagc tgagtttaaa
    16261 ggagctgaat atactctctt tactgcaaaa gatggtcaag cggtcaaatg gagtgaggct
    16321 tttaaaccag agttagtgaa gggaacgaaa gcttctgatg aaacggtgac tttgacttta
    16381 gatgaaaaga atcaagttgc tgttaaacac ttagcgatta atgagtatta ctggcaagaa
    16441 accaaagcac ctgaaggata taccttggat gaaacaaagt accctgtttc catcaaaaag
    16501 gtggatgata acgaaaaaaa tgccgtgatt acccgagatg ttacggcaaa agaacaagtc
    16561 attcgctttg gctttgattt ctttaaattt gctggatcag ctgctgggac tgccgaaact
    16621 ggcttcaatg atctgacctt taaagtatcg ccattggaag gaaccagtga gattacaggt
    16681 gccgaagatg aagcaattac agcttataac gagcaactag gttttgatgg ctatggcaag
    16741 tttgaaaatc ttccttatgg ggattattta cttgaagaag tagaggctcc agaaggattt
    16801 caaaaaatta cgccattaga aatccgttct acttttaaag aaaataaagc ggattacact
    16861 aagagtgaat acatttttac gattaccgaa aaaggacaaa aacaaccaat taaaacggtg
    16921 accgttcctt acgagaaact gacgaataaa gcattttctg ttagcttgaa tcgtttgatg
    16981 ctctatgatt tacctgaaga agaagatagc ttaacttctc ttgggacttg gaaagacgga
    17041 ggcaaagaat tgacagacct tgattctacc gagctagttg ataaattgag ctataacttg
    17101 catgaaatca aagaagactg gtatgtggta gcgcaagcca ttgatgtgga agcgacaaaa
    17161 gctgcccaag aaaaagatga aaaagccaaa ccagtggtaa ttgccgaaac aaccgcaacg
    17221 ttggcgaata aagagaaaac aggaacttgg aaaattttgc ataaattaac cgcagaacaa
    17281 gttttggata aaaccatcgt cttattcaac tatgtgtatg aaaacaagaa accctatgaa
    17341 gcaggtgatg atccagtagc gaaagatgct agcttgaaca atcaagccca aactgtcaat
    17401 tgtacggtgg aacgccatgt ttccatccaa acaaaagccc acctagaaga tggttcgcaa
    17461 acctttactc atggggatgt ggtggatatg ttcgatgatg tgtcgattac ccatgatgta
    17521 ctggatggct caaaagaagc ttttgaaaca attctgtatg cattacttcc ggataatacg
    17581 accaaagaaa tttggaaatc tggcaaaatt gattatgaag tgaatgacaa agaattcact
    17641 aaaaccgtac ttgcggaaaa agtggacaca agtaaatatc cagaaggtac aagttttacc
    17701 ttcaaagaaa ttaactatgg caaagacgga aacatcaatg ggaaacacaa tgaggatcta
    17761 aaagaaaaaa ctcaaacttt aacacctaaa aaagttccga caacaccaag tgcaccagaa
    17821 caaccggaaa taccaactgt tccaagtgac tctcaagaat ctagtcctac agtaaagaca
    17881 ttcccacaaa caggggagaa aaattctaat gttctgctgt ttattgggtt taccttgatc
    17941 tttgcgactg ctgggtatta tttctggaat cgccgaaact aaggtgatgt gatgaaacaa
    18001 ctaaatagga aaaaccaacg tgcgcgaccg cctcctaaaa aaagtggtcg cgctattttt
    18061 gtatccaaaa ctaaaaatga aaagaggaaa agaagatgga attgaaattt gttgtgccag
    18121 atatggccga aacctttgga aagataaggt atgccggcga aggcgaagtt ttaactgaag
    18181 gatatgggcg gaatactaca gtaattggtc gtagttatca tttgtattcc agtaagcaac
    18241 gagccgatga tattgaagtg gtggtagctg cggaagccgg cgaaaaggat tttgatcaag
    18301 atcaaccact aaaagccgtg aatccccatt tggttgccaa aggctatgag attgagaatc
    18361 gtgggtttac cgactacgtg ttgtatgtcg atgatttagt gaaagcatag gaggaaaaga
    18421 agatgagatt aacagaaggt attgttgtag attcaggact aacgtttggg aaattacgat
    18481 tttccgcatt acgccgagaa gtacggaagc aaaatgagga tggaacggtt agtaacgagg
    18541 taaaagaacg gacttataat ttgaaatcct ctgcgcaagg tcgaatgatc caagttagta
    18601 tcccagcaaa tgtaccctta cgcgagtttg cctatgacgc agaggtagag ttggtcaatc
    18661 caatcatgga tacggtcgcc aattatgttt tccgagaagg aactactgtc aattggttca
    18721 ttaaggccga tgatttagtc ttgaaacgac aaccagatca gggaaattct acaaaccaaa
    18781 atgaaggaaa gaaataggtg aagcgtatgg aaattaatgt tgaacatatt ttagattgtc
    18841 tcgatcaata tggaaaaggt gagctaacgg aggaacaact cactaaagca ttaacctatg
    18901 atgaaaaaat gtttctgatt atgcatcaag ggttactgga agtaggtaac gatgtcgaag
    18961 aagattttga tgtactgaat tggttagggg aagaagagtc cttctttatg gttatcgaag
    19021 taaatgaaag tctatgtcga gaagccgaaa ccgtattgga agaaattggt gtggaaatgc
    19081 cggatgccat tgaagttttt ttaaagcaac tagtggaaac gaaacaactc cctatagcag
    19141 taaatgacta gcataactgc ttgaaattgg atcaatgaaa tcctatgaag cttttttcga
    19201 ttggaggtgg aaaccataaa aatgtacaaa gggcatcgta tacgggcagg agatcagcac
    19261 ttggtttatc actttgttct tggctggcta ctcgctctgt tcatcggttg gatgagcgtc
    19321 ttttattttc aagagcttag acaatttgat atttctaagc tatcactttc tactatagaa
    19381 atcgttcgtt ctataaaaga tttaatctat ttgctaggaa gtctcgttct ttcaggaagc
    19441 acgatgttgc tttatataca cttttttcag gatcattggc gcagtttgtg gcatcgacaa
    19501 aagctagccc ggatgattct agaaaatcat tggtacgaag taaaacaaac tcagagtgaa
    19561 ggatttttca aagacctcaa tagtagtcga accaaagaga caatcagtta cttccccaaa
    19621 atctattacc gtatgaagga tggtttactt tctattcgcg ttcaaatttc actgggaaaa
    19681 tatcaagatc agcttttaaa gctggaaaag aagttagaaa gtggtctata ttgtgaacta
    19741 gtagaaaagg aactcaaaga ctcctatgtg gagtacactt tactgtatga tataattgcc
    19801 aatcgaattg ggatagacga agtagtggca gaaagtgggg ctttgcgatt gatgaaaaat
    19861 caagtatggg cttatgattc tttacctcat atgttaatcg ctggcggcac aggtggtggg
    19921 aagacttact ttttactcac tatcatcgag gctttattaa aatctgatgc cgagcttttt
    19981 gttcttgatc ccaaaaatgc ggacttagcc gatttaggta cggtaatgcc tcatgtctat
    20041 tcccaaaagg aggaaatttc tgcttgtgtg gaagatttct atgagcgcat gatgactcgt
    20101 agcaaggcaa tgaaagaaat gcccaactac aaaacaggag agaattatgc gtttcttgga
    20161 cttccaccaa actttttaat ctttgatgaa tacgtggttt atatggaaat gttaacgacg
    20221 aaggaaagtg cggtgatttt gaataagctc aaacaaatcg tgatgttagg tcgtcaatct
    20281 ggtttctttc tgattctagc ttgtcaaaga ccggatgcga aatatttagg agacgggatt
    20341 cgcgatcaat ttaatttccg ggtggctttg ggtcgtatga gtgaactggg atattccatg
    20401 atgtttggcg aggttgataa aaaattcttt atgaagcgta tcaaaggtcg aggctatgta
    20461 gatacgggag ggagtgtgat tagtgagttt tatacgccac ttgtaccaaa aggatatgat
    20521 ttcttaaggg aaattagcga cattggtgga ttaaacgtta atacggaagg ataaaataat
    20581 agtatgtagc ctaggagtgt caagaatcct gggctttttt tacgaggtga tttatgacat
    20641 ttttagattt attcgctggg attggtggct tttgcttagg tatggaacaa gcgggtcacc
    20701 agtgtatcgg cttttgtgaa attgatgatt ttgctcgcca gagttataag gcaatccatg
    20761 atacaagtaa ggaggtggaa atgcatgaca tcacaagtgt atcagacgag tttattcaat
    20821 ctctcggacc agtggatatc ctttgcggtg gatttccgtg ccaagctttt tcaattgcgg
    20881 gaaagcggca aggattttcc gatactcgag gtactttatt ctttgaaatc gctcggttcg
    20941 ccgctattct ccaacctaag tttttattcc ttgagaacgt ccggggatta ctcaatcacg
    21001 aaggaggggc tacgttcgag acgatcctcc gaacgctgga tggattgggg tatgatgtgg
    21061 aatggcaagt gcttaactca aaggcctacg ttccccaaaa ccgtgagcgg atcttcctta
    21121 tcggacattc tcgagacgca tgtaccgaac aagtatttcc tatcattgga tcgtctccaa
    21181 catctgatca aaacatcaga aacttattaa atatcaaccc ttccaatcga ggaatggggg
    21241 gacaagtcta tgggtcagat gacgtagcgc ctaccttgac cggtgatgaa ggaatcaaaa
    21301 tcgcattgcc ggtagatgat ggaatttctg tagcgggtat gttactggga aattttgaac
    21361 aaggcaatcg ggtttatgag ataactggaa cagcgcctac tttgtcaacg aaacaaggcg
    21421 gaacgaagat catgcttcgg aaaaacgaaa cagaatatca agaggtgaag ccagggaaca
    21481 gtgtgaacct ggcttttcct aattccacaa gtcgtcgagg ccgtctggga aaacaaagtg
    21541 tgcatacctt attaacagga gaccaacaag cagtcgtcac ggatcagtat caaattcgaa
    21601 agttaacacc tagggagtgc tggcggcttc agggctttcc ggactgggct tttgatcgag
    21661 cttctcaagt caattcagat agtcagttgt ataagcaggc tggaaattct gtaacagttc
    21721 cggtcatttt tgatattgct agaagattaa aggaggtaaa aacagatgat ttatgatgag
    21781 ttgattgggg aatctactgg gtgattggca agattcagtc agatccggaa ttggaagaag
    21841 aactgtaccg gctcaatttt gatattcgca aaaacggggt gaaagtccct gatgatccgt
    21901 atgtaattga tgaggaaacg gatgctcgaa tggagctgaa tcaagtcatc agtgaattgg
    21961 aacgaattgc ggactttgct aaagaacctg atatccgcca atacttgttt gagataaaag
    22021 cagagttgga aattgagggt attaccgcgg agtaacggcc aaataaacca ctgataggca
    22081 ctgaccgcgg gggatttgcg aagcagaaat cctccgtggt gctgtgcctg tttatggacg
    22141 gcgttagccg gccatgatgt ctcacccccc gtatctaaca ggggggtaca aaaacacaaa
    22201 aaataagcaa gcaacgataa ggaatggctg tcctatcaag gataataagc aatttgtgta
    22261 gatgttaact gatttgaaaa agttaacacc ttaaatttgg aggtgagaac ttggcacaaa
    22321 ggaatttaga ttatcggcta ttaaaagacc ggcgtaatga atatggcgtt tcacaaaata
    22381 aattagctac gacttgtgga cttagtcgcc cgtacttaaa tcagatagaa aatggtggcg
    22441 taaccgcatc gacaaaaacc atgaggaaaa tttttgacca actggaaagt ttcaatcccg
    22501 agttaccttt aacgttactg tttgactatg taaggattcg ctttcccaca acggatgcga
    22561 ggaaaatcat tcaagagatt ctccatttga aatttgatta tatgctccac gaagattacg
    22621 ccttttactc ctatcaagaa caatatgtta tgggagatat tgtggtgatg ttgtcacatg
    22681 aagaagataa aggcgtcctt ttagaattaa agggtcgtgg ttgtcggcag tttgaaactt
    22741 ttttactcgc ccaaaagcgt agctggtatg actttttcga agattgtcta aaagccggtg
    22801 gcgtgatgaa acgcttagac ctggcaatca atgatcgagt tggcctgtta gatattcctg
    22861 atttaacgaa aaaatgtcag aaggaagaat gtatctcctt gtttcgtacc ttcaaaagct
    22921 atcgttccgg ggaactgttg aaacctgatg ataaagatgg tatgggcaat actttgtata
    22981 ttggcagtct taagagtgag gtctatttct gtttgtacga aaaagactat gagcaatata
    23041 tcaagttagg gattccttta gaccagaccg aaacgaaaaa tcgttttgaa atccgactga
    23101 aaaatgatcg agcttatcat gcaattcaag atttgttaaa aggtcgcagt attgaaagta
    23161 ccacattttc cattattaac cggtacttgc gatttgcgga taaggtggaa ggcaaaaggc
    23221 gaactaactg gcccttgaat gaacaatggg ggcgcttcat tggacggaat cagaaggaaa
    23281 ttcaattaac ttctgaaccg aagccttata ccattgaacg aacgttgaac tggcttggac
    23341 gacaagtcgc ccctacgtgg aagatggcca aggagttgga tcggttgaat cagacaacgt
    23401 acattcagga tatggtaaat aatactcgac tatcagaccg gcacaaaaag attttggagc
    23461 aacaaagtat ggcaattgaa aatttgatta tatgaaaaga ggaatcgtga tggaaattgt
    23521 gaaagatatt ttattggtgt ttggtggcag tatgcttggt gttactgtga tgtgcttgat
    23581 gcatgcaagt gcagcggcgg accgagcaat ggaaaagaaa ggaaagtgat gaaatgaatt
    23641 tcggtcaaaa ttt
//
LOCUS       DANKEF010000039        23218 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000039 DANKEF010000000
VERSION     DANKEF010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.039"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(83..283)
                     /locus_tag="PWZ48_001787"
     CDS             complement(83..283)
                     /locus_tag="PWZ48_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2261444.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(736..1056)
                     /locus_tag="PWZ48_001788"
     CDS             complement(736..1056)
                     /locus_tag="PWZ48_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261445.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1559..2677)
                     /locus_tag="PWZ48_001789"
     CDS             complement(1559..2677)
                     /locus_tag="PWZ48_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261446.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            3148..4455
                     /locus_tag="PWZ48_001790"
     CDS             3148..4455
                     /locus_tag="PWZ48_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2261447.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4576..5775
                     /locus_tag="PWZ48_001791"
     CDS             4576..5775
                     /locus_tag="PWZ48_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDL2261448.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5802..7070)
                     /locus_tag="PWZ48_001792"
     CDS             complement(5802..7070)
                     /locus_tag="PWZ48_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HDL2261449.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(7240..7881)
                     /locus_tag="PWZ48_001793"
     CDS             complement(7240..7881)
                     /locus_tag="PWZ48_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HDL2261450.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(8290..8793)
                     /locus_tag="PWZ48_001794"
     CDS             complement(8290..8793)
                     /locus_tag="PWZ48_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HDL2261451.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8916..9680)
                     /locus_tag="PWZ48_001795"
     CDS             complement(8916..9680)
                     /locus_tag="PWZ48_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HDL2261452.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9788..10063
                     /locus_tag="PWZ48_001796"
     CDS             9788..10063
                     /locus_tag="PWZ48_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDL2261453.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10623..11573
                     /gene="yidC"
                     /locus_tag="PWZ48_001797"
     CDS             10623..11573
                     /gene="yidC"
                     /locus_tag="PWZ48_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDL2261454.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11619..12020)
                     /locus_tag="PWZ48_001798"
     CDS             complement(11619..12020)
                     /locus_tag="PWZ48_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2261455.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(12034..12843)
                     /locus_tag="PWZ48_001799"
     CDS             complement(12034..12843)
                     /locus_tag="PWZ48_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2261456.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFVALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12830..13720)
                     /locus_tag="PWZ48_001800"
     CDS             complement(12830..13720)
                     /locus_tag="PWZ48_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2261457.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(13733..14212)
                     /locus_tag="PWZ48_001801"
     CDS             complement(13733..14212)
                     /locus_tag="PWZ48_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2261458.1"
                     /translation="MAIIGVRIDGRLIHGQVANLWTTKLNISRIMVVDDEVATNTIEK
                     SGLKLATPAGVKLSILPIEKAATNILAGKYDSQRVLIVAKKPDRILSLVEKGVPIKEV
                     NVGNMSQTNETRSITKSINVVDADIEVFKKLDQLGIHLIAQMVPNDKAEDFMNLLIK"
     gene            complement(14388..15329)
                     /locus_tag="PWZ48_001802"
     CDS             complement(14388..15329)
                     /locus_tag="PWZ48_001802"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HDL2261459.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(15326..16321)
                     /gene="lacD"
                     /locus_tag="PWZ48_001803"
     CDS             complement(15326..16321)
                     /gene="lacD"
                     /locus_tag="PWZ48_001803"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2261460.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(16314..17510)
                     /locus_tag="PWZ48_001804"
     CDS             complement(16314..17510)
                     /locus_tag="PWZ48_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2261461.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(17528..18256)
                     /locus_tag="PWZ48_001805"
     CDS             complement(17528..18256)
                     /locus_tag="PWZ48_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2261462.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(18697..19671)
                     /gene="lacD"
                     /locus_tag="PWZ48_001806"
     CDS             complement(18697..19671)
                     /gene="lacD"
                     /locus_tag="PWZ48_001806"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2261463.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(19668..20126)
                     /locus_tag="PWZ48_001807"
     CDS             complement(19668..20126)
                     /locus_tag="PWZ48_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2261464.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(20123..21586)
                     /locus_tag="PWZ48_001808"
     CDS             complement(20123..21586)
                     /locus_tag="PWZ48_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2261465.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(21568..22500)
                     /gene="pfkB"
                     /locus_tag="PWZ48_001809"
     CDS             complement(21568..22500)
                     /gene="pfkB"
                     /locus_tag="PWZ48_001809"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDL2261466.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            22642..>23218
                     /locus_tag="PWZ48_001810"
     CDS             22642..>23218
                     /locus_tag="PWZ48_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2261467.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         6976 a         4972 c         3900 g         7370 t
ORIGIN      
        1 actctcatcg ctttttaaag aaaatagaca aaaacgccca gacacgcaaa tgtctgggcg
       61 tttatttttt agtataggtt aattaagctt tagtaacgtt tgttgcttga ggtccgcgtt
      121 ggccttcttc aacatcgaaa gttactgctt ggccttcttc taaagttttg aatccgtcgc
      181 cttggatagc tgagaaatga acgaatacat cgtttccgtt ttctgcagtg atgaatccaa
      241 atcctttttc tgagttaaac cattttactg tacctgtttc cataataaaa atcctccttg
      301 tgtatcaaca catattttgc aattacttga atggtgtata tctggaagat gtttatttga
      361 aacaagctac cacagattac cgaacaaaaa ctactcctat agtatatcat accgtttaca
      421 aaaaagctag agtttttcga ctttttttat taaaaaaagt tttctcagcg attacataaa
      481 gtgaaaaacc gaactttttt ttaaaataaa cgacatattg ttcagttggt gtttttttgc
      541 tctgatgttc ttttttaaac taaaaaaatt attctttctg actacttatc ggttctgttt
      601 caacggtcga ggctggggaa gattctttag taggtctgat gttctcatgg tatttctgat
      661 agcctatgac agttaccaga agaacaaggc aaaccaatac gaccaacgag attagttttt
      721 cttttttgct catatctata cctcataaat aacaggatga tgtccatcat atttctgagt
      781 atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa tgccagcaac tttcataatc
      841 acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc aaagaccgtt cttctgtttc
      901 aggaatcgca gaatcaagat ctggtgtctg tacataaaca tttttgtaca gacgtgtgcc
      961 tgtaatagga aaccgctgtg tttcttcagc aaaaactggc tgacttgtta aacttaggga
     1021 tagagacagc aaacaccata acagtctttt tttcataaag acctccttat cgataagttt
     1081 ttcccaatta aaatcatatc tcaagaacgc ccgttattca cattttgttc ataaatgatg
     1141 ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg tcacaacctc ttttttaaac
     1201 ggtgttccag aagtagcttc ttcggaaata agccgaaatt tcacaaaaat ttgagaaata
     1261 atttttgtca atctcttctt atttcttgaa gctgaccact tctgtcacaa cctcttcctt
     1321 aaacggtgtt caaaagcttg catctgcggt aataagccca ccaaacaaaa aatatgaaga
     1381 gctatttttg cttgctgttc ttattacttg atgcagaaca gctttttcac aacctctcat
     1441 acacggtgtt taatcagctg accttcggta ttagctcaaa aaacggcaaa atatgtgaaa
     1501 ctatttttcc gtttgtttct ctaatactca ggtcaaaaca ctgatttcac aacctctttt
     1561 aataacgaag aatcgctgtc agactctttt catctggcgc atctttttga tccaaaataa
     1621 acacttttcc gccattaatg atcacatcat ctgctaatgc attcaacact tgacgccgat
     1681 catattctgt ttcaggatct tcaccgaaac catcagctaa gtttgacgta gcgataaata
     1741 aatgtgacac ttttccttct ttcgcagctg gaacgatatc aactaactga tcgatgaatt
     1801 tcttatctaa gagtttattg tattcttttg attcttgagc tgcgagttca cttgacagct
     1861 tcttcgcccc ttcttcgata tctctaaagg atagctgcgc aggagaagca gaaatcgcaa
     1921 cgtctgcaca ataataggga tttttagcaa tttttttgaa catcgtctga ttttctggta
     1981 gagcaaataa ataaatcggc cattgttctg gattttcgaa agaatccttc aagtacgtat
     2041 caactgcttg gtaatagttg acccaatcga tctctacttc ttcatctttt ggatttacgc
     2101 catggaaagt tactccatct cgggctccgc ctcctccatt agaagagaag ttcaaaccgc
     2161 cacctgtcag ttcagatccc aatgcagtaa cgacatcaac cggtgcccct tctggtagag
     2221 atacttcttt tacttgtttg ttgctgactt catacaattt catggagtcg cggttcaatc
     2281 ccaataaata ataagaatag ttgaactgcc catttttgat aatagccagt aaataaggaa
     2341 gatcacttac atagtattga tcgtctaccg ggatactcaa acgatggata taggtttggt
     2401 cagcagttat aatgaccgct acactcgtag ttgcactacg ccaaaacgaa ggatctccta
     2461 acaaattttc aattttttct tggaacgggg accagtcatg ttcggaatat tttttctcaa
     2521 aacgtttctt tgcttctttc gcaaaatttt tgaatttgat ctgatctttt tccacatctt
     2581 gatgggcgac atgcgtattc agcatgatcg tgacgaatgg tcctttagcg ccttctgaag
     2641 tcaattgtga caataattct ttatcctttt ttgccataaa cgtacccctc ctaaaaaaat
     2701 cattcctttg tttctatcta aagtctacca caacactatt tttccataca ataaaacgta
     2761 tctaaaaaaa taaaacggtt tctacggttt taaatttagc tattttttta agttatcttc
     2821 ataatttcct caattattta gccccagtct tcaactattc ttcatgaaat gctgataaac
     2881 taagaaaaag aataaaagag gtggatctta tgcatttgat tatgattttc agtttattaa
     2941 gtattatcag tttatttgct ttcagcgctt cccgcttatt agatgcggaa acacagccta
     3001 ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat aacgtgcgtt cttatcctaa
     3061 agttttttca gaaacattga aactctctcc cctctctgat aaaattttaa tttgtagcga
     3121 aacaaattaa ttacaaggag atcggatatg tccataaaaa aaacacgtct agtcctatta
     3181 ttggctagtc tcattctttg ccttgcttct tttctaccag ctgttgctgt gcaagctgaa
     3241 gatactttca aagtaaatgc aaaagcagcc tttgctgtag atgcgcaaag cggtaagatt
     3301 ctttatgatc aagatggaga aaaaaccatg gggatcgctt caatcacaaa aatcatcggt
     3361 ctttacatcg tccttgatca agtaaaagaa gggaaactat catgggatga taaagtttca
     3421 atttcagatt atgcagaaaa tttgagtgtc accccagatt tatcaaatgt tcctttgcat
     3481 aaagaaaaca cctacaccgt taaagaatta ttcgactctg caatcatcca gtcagccaac
     3541 gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg aagcaaaatt cactgaacga
     3601 atgaaagaac aattgaaaga ttggggtatc aaagatgcaa cgatcgttaa tgcatcagga
     3661 ctgaataact cttatcttgg cgaaaaccga ccagaaggta ctggagaaaa cgacgaaaat
     3721 caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt tgatcttgga ttttccagaa
     3781 atactggatg tatctagcac tacgacacaa atgtttggcg aaaacacgca atcaccagta
     3841 gaaatggtca actggaactg gatgctccca ggattcgtga attataaaga aggcgtggac
     3901 ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt tcgtcggtac gatcactaaa
     3961 aacgatcaac gaatcatcac tgtcgtattg aatgcagacg gccatgcgga aaacccaagt
     4021 gcccgtttca atgaaacagc tcgcttaatg gattattgct acgacaattg gtcagaaaaa
     4081 gagcttggaa aagccaatgc aagtatccct agtctaaaaa ccattgatgt aaaagacgga
     4141 aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa aagtatgggt acggaacgat
     4201 atggatactg gaaaattaac aatcacacca actcttgaca aaaaacaagt cactgacaat
     4261 gagatccaag cacctatgga taaaggaaca aaaatcggta ccgcaacgat caccttggca
     4321 gaagataaat taggttatct tgaagacaac aacgcgccat ccacagaaat catcacggat
     4381 aaaaccgtag aaaaagcaaa tattttcgtt attggctggc gacgcgtcgc agaatttttc
     4441 ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt tccatgtgga agatgttttt
     4501 ttgctttttc tagtttaaat aacagctttt ttcatctatt ttcgttatac tgtataagaa
     4561 aaaggagttg aatttatgaa tctaaccaga gatctgattt ttttggttct ctatcttatt
     4621 ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg atccttcctt gttcttttac
     4681 agcagtacat ggacgtttgc ggtaatcttg acttattttg tcgtgttgga aattttttat
     4741 tcgacctttt attttttgat tcccttagtg tttttcagta tctttgcctt ttcctatttt
     4801 tcagaaaagc gaaggctgtt gaatggtctt ttattcaata ttttcctcat tagttttggt
     4861 atctacttgt ttgtcttgct atacgaaaca caaaatattt ttcttggcgg actgattgcc
     4921 ttgatcacga tcccgttact gcttgtcttt ctattcggca tttatggatt gatcgttttt
     4981 cttttttgga acggtgtcac agttttacgt agagagtcgc attcattagc taatctttta
     5041 acgttgatac tagccatttt tcttacattg tttcttgttt ttgacttttt cttgttaaaa
     5101 tatttaccac aatggataaa tgctttgttt ttctgcatcc cattgatctt gatctatcta
     5161 tttatcgtat tctacaactt tttgactgtc tcattcttgt atcagttcaa ccgccctcga
     5221 tacaaccaag acttcatcgt tgtgttaggc gccgggttga tcaatggaga aacagtttct
     5281 cctcttcttg ccaagcgaat caataaggcg attgcttttt attgggcaca aagccgtgcg
     5341 acattaaatc cgcctatcct attaatgtcg ggtggacagg gagcagacga aaaagtacca
     5401 gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa ttcctgaaag agatattctt
     5461 gtcgaaacca attcgacaac cactttggag aatatgcgct attcaaagga aatcatggat
     5521 cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca acaattacca cattttcaga
     5581 gctggtctct atgcacgaca agcgaaactg aaggcaaatg gcattggggc aaaaacagct
     5641 ttctattact tgcctaacgc gtttttaaga gaatacatcg ccatcttgat gatgaacaaa
     5701 aaaagacata tgatcatttg cggattgctt ttcgcaggtt ccgcattctt atcactgatc
     5761 actttacttt tctaaaaaaa caaggtgtga caaaacatat gtcacacctt gtttttttct
     5821 tttattctct tgacgattgg caagaatagt attccttttt tgacggctac ttgctgtgca
     5881 gaataaatac cgctgtttcc tgaacacata aagctgatca agcagatcat aaagaagtaa
     5941 gcagcagctt gtgaaccaaa taattcgatt cccatcacaa aacaggcaat cggtgtattt
     6001 gtcgcaccag aaaatacccc gataaaacct aatccagcaa gaaaaggaac agaaacatgg
     6061 agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct caaatagtgg tgtcacttct
     6121 cccccttggt accccgcacc taaagacaag accgtgaaga ataatttccc aataaaatca
     6181 taagcatgtg cctgtccatt aaatgattcc tgcagtaacg gaagactcaa cccaaggtaa
     6241 cgttgtgttt gcaaaatcaa cgctgcggct acaactactg ctccacctat cgtatttcgc
     6301 caaacaggat gaggaatcca ttgcgcatac cgcgctttta aaaagacgat cgaacggctg
     6361 aatacccaac caatcaaacc aaaagcaatc ccagcaacaa aaactttgaa gaacagcaac
     6421 atactccatt caggaatcac acccatttgg taatgtagat gagaaacacc atagctttct
     6481 gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa atattgcttc tgttcggact
     6541 ttaccgattg ccaacacttc taatccaaat aatgttccag ctaaaggtgt gccaaataca
     6601 gaactaaaac ccgcactgat tccactgata accaaaatct cccgttctgc tttatccaat
     6661 cggaataaat gcgctatatt atcagctaat gctcccccca tttgaactgc tgttccttca
     6721 cgaccaacag atcccccaaa taaatgggta gtgatggtcc caaacaaggt caatggaatg
     6781 agccgcaaag ggatcttctc ttctcctcca ttcccttgat cgatcactaa gttattccca
     6841 cgactagaaa gaccgccatg atacgcataa agataagcaa aaagcgcgcc gctgactggc
     6901 aatagataaa gtagccaagg atgtgctagt cggatatctg tcacgaacgt caggctattt
     6961 aagaaaaaag cagacaagct ccccatgaat actccgagaa tcgctgtgac caccagccac
     7021 ttcaacatgt acatcaaaat acgcactgga ctcggtaatt gtttttccat aaaaaattcc
     7081 tcctcaaata aaaaaacgcc tactaaagaa aggggctggc cccagacttt agtaggcgtc
     7141 attagctatc ttacgttagc atttaaaggc gaacaccatc acctattcac ttccattaaa
     7201 agatagcata tttttctgta aaaaacaata aaattatcgt tatttcttta cacgttcagc
     7261 aaattcatag aaaggagcca atttcgttaa ctgttgatta cattgatcac tatcatgaca
     7321 aatataattc ccctttttcg tatacgtgcc atcagaaccg gctttcgttg tagaaagaaa
     7381 gagcgagaca ttcgatgttt tctgacagat cgcacagatt cccttattaa tcgttggtga
     7441 taaagtaccg ctgatccctt ttaattttcc atcttcgtaa taaaccatga attttttttg
     7501 cgtccctgga tcattccaac cgatgtacgt atgtgcttgc aagtcgattt cattccaagc
     7561 gggttgtttg agttttttca cttttcggaa taatttctcg atttgtggtt tgctcggctg
     7621 cataaacggc tggacgattt gcttcagctc ctccaatgct cgttctgctt gcgtttttgt
     7681 cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt tctgatggtt cgtaacctaa
     7741 tacatcgcga atcttttcag ctgctagagc ctgaaccgtt tgtaccgttt ttgagtcatt
     7801 gacagatgag tacgcattta ataactgctc tacttctctt tttataaaaa aatattgata
     7861 tggtttcatt gtttgttcca tgattttaaa gtcctttctt ctaaccaaag tgcatacttt
     7921 gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga agatcccgga ctcttttaaa
     7981 taagatacgt tttgcatctt atcatcaccc cctttaatgt tcattcttct atgtacatga
     8041 ttcattcgat aaataaaagt aaaccgtatc ttgagcagat agtcaactgc aaacagaaaa
     8101 aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa acaaacggtg ttccagaagt
     8161 agcttcttca aaaaataagc cggaatttca caaaaatttg agaagcaatt ttcgtaaatt
     8221 ccttcttatt tcttgaagct gacccacttc tgtcacaacc tcaatatggt ctattattgg
     8281 ttttacctgt taaatcgagg attcttttcc gaagtaacgt tgccatctct tacgcatact
     8341 tgctgacata ggctgagcgg cttctttgac tgcgcagtat ttgtctacga atgttacgat
     8401 atatccttct ttgtacttag gcggtgcaat cgttgcgccc cacatgtgct tcaaaatgat
     8461 atctcgctca agatccgaca aatcggtgat cttttctgca ttctttacag cgattcttgg
     8521 atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta cgccaatcat agtagaacag
     8581 atcatgcaaa agtccagcac gcgctgttgc tttagcattg ccaccccatt tttttgcgag
     8641 cagataacta tagtaagaaa cactgatgga atgctccaaa cgagtggaat gaacatgctg
     8701 tgtgtaattc gctaattttt tcactgcttc tgtttctaaa agatcttcta cataagacat
     8761 atattcttcg tcttcacgcc atttttctgt cataattatc gatctgccac ctttttgaat
     8821 ttcattttct tctgaaattg atctaagtat accaccagtg ttcggattat tctagtcgcc
     8881 ttggataaaa tagccaaagc ttaatagttc cttaatcata aagaaaaatg ataaagaaca
     8941 atccctgccg caaccccaac attcaacgat tccgcatttc ctttcatcgg gatatatacg
     9001 ttctgatcag tcaaagacaa gatttcctga cttaccccct gaccttcgtt ccccatgatt
     9061 attgcgaagt tatctgattt ttctactttg tctaaggcgt gtgcttcttc atttaattct
     9121 gtgccataca ctgtaaaccc tttgtctttc attaaaggaa tcacttctgt caaatctttt
     9181 tgcaacacag gcaaatgata attgcttccc tgcatgctgc gcagcacctt tgtactataa
     9241 atatctgccg ttcctttgcc taataaaaca ccagccatat caaatgcatc tgccgtacgg
     9301 atcatcgtcc ctacatttcc aggatcttgt acattatcta ataacagcca tcctcctgaa
     9361 aaatctggcg tgccttcagc cgttaacgcc atcactgcta agattccttg tggtgtaggt
     9421 agatccgata cagcatccat gacctcatcc gaaactagaa aagtagaaat ctctttgttt
     9481 gcggccagcc agtctcccca ttctttcatg cctcgctgtg tcacaaaaag ttcttttatt
     9541 tctgcatcag acgacgcagc ttcttcaatc agatggaacc cttccaaaag atattcttcc
     9601 ttttcttccc gatatttctt tttatgtagt ttttttaatt ctttgattct tggatttttt
     9661 gctgattgga tctctttcac attcctcacc tccccatgat tatatcatgg aataggtgct
     9721 tgagcttgtt tcatcctaaa aaaatcggta tcattaattt ataaaatagg aaagaaaggt
     9781 gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc gtccaaggcg taggatttcg
     9841 ctacatgacc aagatggtcg ctgatcaact aggcgtgact ggaacggtaa aaaacgaaga
     9901 tgacggctca gtcacgatcg aagcaatcgg caatgacgac atcatccaga aatttattga
     9961 agaagtcaag aaatctccga gcccaagtgg acgagtgcag tatgtggatg tccaagaaga
    10021 cccgatgatt gaggaacgaa agaaatttga tgtggtggga taaggttgtg aaaaagctgg
    10081 tctgcatcaa gcattaagga caggttgtga aaaatagctc tccatatttt tcaacaggct
    10141 ggacttaatg ccgtagatgc aagcttttga acaccgtcaa taaggttgtg agaaagtcgc
    10201 ttagttttga gtagtaagga gaattttgtg aaaatggctt tccacatttt tgcataaaat
    10261 ttgacttaat accgagaaac tgacttttga acaccgtcga taaggttgtt tttgagacgc
    10321 tttgcctcaa gtattaaaga taggtagtaa aaaagctgat ctataacttg caataaattt
    10381 gcttaaccca tattttccag acagaacaaa aaagttttta gttttttaaa taagaaagaa
    10441 acacttggaa tcactctttt ctttccaagt atttctttct tgattttttg tctttttttc
    10501 aaaatttttc gctacgtaaa agaacgaata tttatatgaa atttctatga agttgattga
    10561 attccatgcg cttttttagt atagttaagt ttgtgtaaaa attatagaag aggaagtatt
    10621 ctatgaataa atggaaaaac tggttattgg gatccggact tgtcagtatc ctgctctttt
    10681 tatctggctg tgtgcggatg gattcaaacg gaaaccctga cacttcggga atcgtttatc
    10741 gagtactcgt ccatcctatg ggccaagcga ttacatatct agttgagaat ttcaattggt
    10801 cttatggctg ggcagttatt gcaatgactg tgatcgtccg tatcatcatc ttgccactag
    10861 gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa aatgcaagcg ttaaaaccac
    10921 aagttgaagc agcccaacaa aaattgaaag ctgcaacaac acgtgaagaa caaatggcag
    10981 cacaagcaga aatgcagcaa gtataccgcg aaaacggatt gagtatgacc ggcggaatcg
    11041 gctgtctgcc tctattgatc caaatgccga tcttctctgc cttgtacttt acggcacgtt
    11101 atacagaagg tattcgggaa tcatctttct acggcatcga tttaggctca ccaagtatgg
    11161 tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg atacatctca acgatcggta
    11221 ttcctgaaga acagaaaaag acgatgcgta cgatgctaat cgtctctcct gctatgatcg
    11281 tcttcatgtc gatcagtgcg ccagccggtg ttacacttta ctgggtagtc ggtggtgtgt
    11341 tcagctgtct gcaaacattc atcacaaacg ttatgatgaa accacgtttg aaagcacaag
    11401 tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt cactccaaga aaagacgtga
    11461 cagaatcagc aaagccagct aacaacaaac aagcaacaaa acaaatcaaa caaccaaaaa
    11521 acagtacaaa caacaaaggc cgcaacgcag gaaagcagca gaagcggaaa taaaaacata
    11581 ggacaaaaaa gatgagagct aagccctcat cttttttgtt actcttcctc ttctgctatt
    11641 ccattaatta attcgttgac agagacaatg ctttcacatc ctgcacgaac aaactcgggc
    11701 tctgaaccaa tcatttgtga gttgataaat tcaatgacca tcggaagatt catcccagca
    11761 taaagagtaa tctgttgtcc ttccattatt agtttagaaa tgatatttgc aggtgtacca
    11821 cctaaaagat cgcatagtac cacaaaatca tttaacgaat ctgtgacagt gagaaacttt
    11881 tgtttgtatg tttcagtgcc atcttctggt aataaaggta acgtgtagat atcctcttga
    11941 ggccccatga tcatttctgt actttttttc aattcctcac aaaattttcc gtgactaatc
    12001 aataccaatt gtctactcat ttttcgctct cccttatttc gaaatcacac ccaatgcaga
    12061 taaagcaaca cacacaatta atacgataaa aatcgctcga gtaggagtca tgtttttctt
    12121 tcctaataac caataaacac caccgacgat cccagcaggt aaaattttag gtaagatcat
    12181 atcgatatag ttttgcatat tcaatgtcac atcgccaatc gttggtgccc atgaaaattt
    12241 aacattgatc attgtcgcaa ctaatgcccc taccatgaat acccctaata aagtggctgc
    12301 gtccgttaat gcagttaagc gagattgcat agtagtaact aaggaaattc cttctttgta
    12361 tgcaaattca agttgcttcc aacgaaaaac catgacaacg atctgagcaa aaatccatat
    12421 aaacacacct atcggatttc catcagttgc catattcgcc gccaaagccc caaaaatcgt
    12481 tggaatcaaa gcaacaaata cggaatctcc aatcgctgca aaggaaccca tcaatccagc
    12541 tttcagcccc gatacggttt cctttgaagc aattccctct ttttcttcca ttgcaagatc
    12601 aatcccagta atgattgtat taaaaaaatt agaagtatta aaaaattgcg tatgtgtttt
    12661 catcatctct tttaattctg gtgtattgtc tccatatatt tttcttagtt gtggcaagat
    12721 agtatataaa tagccagaac cctgcattct ctcatagttc caacctaatt gaaaagtgaa
    12781 caaacttctt cgattgattt gcttaaaatc ttcttttgtt aatttgtaat tagatttcgt
    12841 catcttcgat ttccccctca acagaatgtg atggctcact agctaatggt tgatggtctt
    12901 tgttttgatc attcttataa ctgatcattg ctagtgctag accaatcaat gctacagcaa
    12961 gcatcggcag cgaattgaaa gttgccggaa gatctttaac aacgcttgcc acacttgttc
    13021 ctaataattg aacattagag aataacgttg ctaataatgc cgtgatcgta aatcctaaaa
    13081 taagataaga tagatgtttt tttactggta aatagcgcag aagaatggca aacccaacag
    13141 ctggtaaaac agctcctgct actgaaagtc catcgcctag ccattttaga ttcccgttta
    13201 aaacagaaac aacattttca actaatccac caccaaatgc taaagctaaa aatacaggaa
    13261 tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa attgcgttcg attgcttgat
    13321 aattcatgtc ttctacgtga cggtcaatac gatgggcaaa gtatgtgtta gcgaatctag
    13381 ctaaaatatc caattggatc attaagcttg ctacaggcac tgcaatagca gcaatcgctt
    13441 gttctggatt cattccaaga gaaatcgaga aggcagttgc taaaatcgtc ccagagttgg
    13501 catctatttt tgatgcgcct ccaaacgtcc ccactcctaa aactgtcaac tgcatactgc
    13561 caccaataat caaacctgcg tttaaatcac ccattactaa acctgcatat aaccctgcaa
    13621 acactggcgc actcattgaa gaatagatct gtaattcatc taaaatttga taacctgcat
    13681 ataatgttaa taataagatc tgccaccatt gaatatccat ggatcattcc tcctatttga
    13741 ttaaaagatt cataaagtct tctgctttat cgtttggtac catttgagca attaaatgta
    13801 taccaagctg atctaatttt ttaaatactt cgatatctgc atctaccaca ttgattgatt
    13861 ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc cacatttact tctttgatag
    13921 gaactccttt ttctactaaa gataagattc gatctggctt ttttgctacg atcaatactc
    13981 gttgagagtc atatttgcca gctaaaatat ttgttgctgc tttttcgatt ggcaaaatgc
    14041 ttaattttac ccctgctgga gtcgctaact tcaatccact tttttcaatt gtgtttgtgg
    14101 ctacctcatc atccacgacc atgattcgac taatattcag tttcgttgtc caaagatttg
    14161 ctacttgtcc atgtatcaaa cgtccatcga ttcttacacc aataattgcc atcatgcatt
    14221 ctccttatct attttttcta tttattgttc aattaaaact gtgacatcat caaatatccc
    14281 ttctgtttca aagataacgt tttcattttt tctttcttat atttcatttt taagctaaga
    14341 acaaattcct tcccaactaa agaaaacgct ttcttttata tttaagttca tttgattttc
    14401 ttaacttgaa tgtgtttcat caacatcgta actttcatag ggtcgatcca gccagttttc
    14461 tcttgttgcg cgtttgccat accacaagcc atagaaaatg cgagaatctc ttcgattgat
    14521 tgcctttttg ataacgcatg ggccgatcct gcaagggtcg cgtcacctga accaacagga
    14581 ttaacaactg atattttagg aataatgact tcgtaaaaac actgagcaac ttttgccaca
    14641 gcacctctct ctcctaaaga aacaacaacc catggcactc cttcaaatat tggatgagct
    14701 aataaggact gaattttttc taattgaatc gaagaacaga tacgttggcc aatcagttct
    14761 cctaattctt ccagattggg cttgataagt agtggcttct ccttaccttc caatacttca
    14821 agcaaaaatt taccagaagt atctagtaat atgtctttcc cttttttatc agctaaagca
    14881 accaattgct tatagtaatc aagagggcat ccttggggta aactacctga gatcgtcata
    14941 atatctgata tttcgaggag ctgctcgaaa tgatcggtaa atgtctgttg ttctttcttt
    15001 gttatgaagg ggcctcgttc caaaagctct gtttgtctcg aactatgcag gatagcaata
    15061 gaatcccttg tctctccttt aattggaaca aactgatgca tgatccctaa ctgatctagc
    15121 tggttcttta gatattcacc gtgctggcca cctattaaac cagtagctat cacctcttct
    15181 cctaataaat ttagaaccct cgccacattc actccttttc ctcccgctgt tttattgact
    15241 gtttcaactc gattgacttt gtcgaggttt aattcttcaa ttgtatacgc gatatcgatg
    15301 gaaggattca aggtaactgt cgtaatcatt tcatcttcac ctttttgctt tttgttttat
    15361 tgaaccaagg ggtagcggtt tcatttatta ctttatttaa agtctcgatg ttttctctcc
    15421 catttgttct taaccaatga atagctccgt cttttccatt ttcgacgtat gctttgaccc
    15481 catctttcca tgtcgcacgt ccacacaata caccattgaa ggtggaaccc gattcatgag
    15541 caaaaatcag tgtttgttga aataattctg ttgaaacacc cccactcaaa aaaatgaatg
    15601 gtaatggagt cgcttcactt tgctccttaa agaaggcagc agcctcttct ttgctgtgaa
    15661 taaattcacc tgtggaatag ccattcacat aattcatatt gactggtact tctactttca
    15721 acacatcaac tttatatttt ggatcagaaa attctttcat catctcaata actttgtggg
    15781 gcttgacttt cgcatactct ggagaagaac tatcgcttat ttgtgcgtca taagaaacta
    15841 actccaaata gaagggacga ttttctcctt cgcactcact acctaaacgt tcgatgaaca
    15901 catgcttcaa atgattgatt tcgggtgctt catcaacatc gtagtaaagt aagaatttta
    15961 ctgcatccgc ttgctgttct ttcaatctga gagaagacca atccgctaat aaatcaggta
    16021 gacgaccagg cgtagttgcg tcatagccgg ttttctcata agctaacaat aaccccgtct
    16081 cttttgctct gacttttgct gctggcaatc catattccgg atccaataaa atagcagaag
    16141 catagggtgt cagctcagct gatactagtt ctttgaattc actgatttgg tcttcacttg
    16201 gctcttttcc caacgatttg atcatttttt ttagagcacc acgctgatcg atcgctaatg
    16261 cactaataat cccatctttt gttgatagtc tctccattgc cctatttttt tcttcattca
    16321 tctgtcattt ctccttcggt ttcatactta tgaataatga cgcccttgac tactcgattg
    16381 accgtgcctg ttggagatgg tgtatctggt ttattagcta ctttgattga agtatagagt
    16441 gcaatgattt gtgcagtcat aatcatcgct aatgcaagat aggcatcggg caatttactc
    16501 accgagtctt ccaatagcac agtcatacct gagaaatcaa tagtacccgg ttgcgcaatc
    16561 gcaatgacat tggcagtgac ccgatcttct tttatttcct ctaaaatatc taagtcataa
    16621 tttcgcgtat atggttgatt gctcacaaaa cctacaacga gtgtctcgct atttaaaaaa
    16681 gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa ttgtagcaac tttacctgct
    16741 gttaattcta aaatttttaa ttgtgcttct cttgtcgcac ctgccaaact tcctgaaccc
    16801 agataagcga tccgttcaaa aaaatcaggt aataactttt gaatttcttg atctcttgaa
    16861 agaagatctt gaccgatttt aactaattgt tcaagatatc tttctttttt ctccattgct
    16921 gtttgatcaa agatccataa agcactcaat aacatacacg taaaacttcc tgtcatagca
    16981 aaacctgcat cgtttgctct ctctggcatc aataacagat aattctgttt atcatcctgt
    17041 gcgttttttg ccaacatccc ctctttagca caagtaatcg tcaaatgaaa caatgtttca
    17101 acttgttcat ttgcaatttg cacagccgct acactttctg gactgttgcc actgcgagca
    17161 aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt cttttggtga agctacaata
    17221 tctgtcgtgc caaaactgtg gaagataaaa aactgattgt ctccatgttc atttagatag
    17281 ggtaagagcg tatccccaat atattgagac gttcctgcac ccgtaaaaat aacacgtatt
    17341 cttcttccgt ttgcttcttt ttttatttga tcaaaaaata gagctaaatt ttcttggctt
    17401 tgcatcaatc tatcgattgt ttctttccag agatctggtt gttgttttat ttcttgtgtc
    17461 gtgattgctg cacctatttg ttttaatttg ttcttcgata gctcaaacat gttgttctcc
    17521 ctctcaatca ttttcggata tgccgaattt tataatgaaa ctggtccgct cttgctacac
    17581 tatctgtata ttcgataatc tcatttttca cgttataggt tatcctcgtc agatgtaaga
    17641 cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt tttttgcacg atacttgcat
    17701 aaaactcttc atcagctaat tgaacttgtt gttcataatc ctgtgaaaac acatcataca
    17761 acggtttact ttcaagtaac ggcaaatcaa gtcccataaa tagtttcgcc ggtagataag
    17821 aacgctcaac catcaatggc tcttcatctg ccaaacgcaa tcttttaagt ttaaatacag
    17881 tttcacctaa atggatgttc aaattcttag caattctctt attagcttcc atttgtctaa
    17941 attctaaaat gaccgtctta gggacgcgcc ctatttcgcg catttcttct gtaaagctat
    18001 atgctccagc taagttcgat gacttatcag acaaatcaga gacataggta ccttttccat
    18061 gtcttcggta aatataacct tgattttcca attcctgtaa agccagacgt acggtggtac
    18121 gactcacccc atagtgttga caaagttctc tttcagataa cagcttatca tgcggagtca
    18181 tctgtgtttc gatacgatcc ttcaataaat caactagttg ataatacaat gattttcctt
    18241 tatttgagct ctccataaaa atcacctctt ctttactggt tataaccaca acacaagaat
    18301 agtgaattat taatcatttg tcaaaggttt tttagaccaa aaaagctatt ataacagctc
    18361 tttaaagctt tatatcttga atagacaaag atacctcaaa aataccagtt accaataaaa
    18421 gaacagaaaa aaagacgaac cacataagcg atccgtcaaa atggtctaac ttatcgagca
    18481 gcgtgatcca cattaatccc tatgatattc ctacaactat cgttttatta cttccaatat
    18541 catttacaac aactaaaaat agcttattaa tgacaaatgt tattattagt tttagataac
    18601 ttttgcagtt ttatactttt tttctataaa atttgacaaa cattttttta aataactaat
    18661 tattttttga atctgtcact cagacaatcc tttctcttac ttgttaacat aaatagaagt
    18721 tgctgtttct tctaatagtg tacttaattc tttaatattt tgttttcctt cttctaacat
    18781 ccaactactc gctttttctt cgccttcggt aacaaaaggt gctactccct tcgcccatgt
    18841 tgctcgccca cataagacac cattgaatgt cgaacctgat tgttttgcaa aacgcaatgt
    18901 atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa ataaacggtt gttcagtcac
    18961 tttcgattgt tcatagaaat acgcggatgc ctctgcttga ctataacaag tatcttgccc
    19021 atatccttcc acaaatctca tattcactgg tacttctact ttcaatacat ccacttgata
    19081 acgttctttt gaaaattctc gcatggcttc aatcacttta tgcggtttta cttttgcata
    19141 gtcaagagaa gacggatcgc caatagttgc atcgtaagaa aggagctcta aaaagaaagg
    19201 tatgccttct gcttcgcatt ctgaaccaat ccgttctata actatttgtt tttgcacatt
    19261 aatctctact ggttcatcaa cgtcatagta caataaaaat ttacaagcat ccgcacccac
    19321 ttcttttaac cttttcactg accaactatt cagtaaatct ggtagtcggc ctggcatttt
    19381 cgcatcatag ccggtttttt cataagctag caacaaacca cattctttct ctcgaagcgc
    19441 tgctgcaggc agaccatatt ctggatcgag taaaatagcg gaagcatacg gagtcaggta
    19501 ctgagaaacc atctgtttaa attggatgat atcttctgat gtagcttctt tatattggtt
    19561 gatcattttc ttcaaagcac cacgttggtc gatagctagc gcgctaataa ttccagcttc
    19621 atccgtcaat cgcttcaaac gttcctgttt ttctatactc attttcttca tatatcttca
    19681 cctatttctt tagttaatat tgtagctatt tctatttttg attttgcagc tctcaactgt
    19741 tctgtaacct cttttcgcat caatgatcgt gcaacgagag acaataattt caaatgagtc
    19801 gttcctgctt ctgaatctgg gataaataac gcaaaaataa aatcaactga ctgtctatct
    19861 aaactgttcc attcaattcc ttttttcagt gtgacgataa tcaaactagg ttcattgatt
    19921 gtttgagact tcgcatgagg gatagcaaaa cctgacatta ttcctgtagt accttctttt
    19981 tctctttcca gaagtttttg atatacttct ttttcgtcat gagcgattcc taaacagaat
    20041 gtttgctttg ctaaaaactt aaatgcttct tcttgattat ctaatggctg ttgtaaaaaa
    20101 atgttctctt catgaatgat tgtcatgata aacctcctgc tcttccttct tcgataactg
    20161 tacgatttgt tgctttatta tccctcttct tgtctttatc agttactctt ttcttttgga
    20221 tcaaacctag tatcactcca ccaacgatcg aaccaataag aatcgctgta acccattgga
    20281 tttttcccgt cactacaggt aacactaaaa aaccgccatg tggggctggt acttggacac
    20341 caaacagata ggtcaagaca gcagcaattg atgagcctaa catcatcgtt ggtaatactc
    20401 ggatcgggtc tttcgcagca aacggaatag ctccttccgt aatatgagtt gatcctaaaa
    20461 taaaattaac caaacctgca ttacgatcat tagtatcaaa atatttacca aacaataaag
    20521 tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc tgacgcacca gccataaaag
    20581 tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata tgccgcctta ttaaccggtc
    20641 ctcccatatc aaacgcacac atacagccaa caatcacgcc aagaacaagt ggattcgaat
    20701 attcaaatcc agctaaaaag ctcatcattc cttcatttat agccttcatt ggacctgcca
    20761 gcaaagacat cacgatgcca ataaatgcta caccaataac tggatataag acgattgcct
    20821 tcaagccatc tagttgttta ggtaaaatta tcagtagctt ttgcaaaaat aagataaaat
    20881 aacctgctaa gaacccagct aaaatacctc ctaaaaatcc agtaccgcca ttcatggcta
    20941 cgattccacc aacaaatcca acgactaacc ctggacgttt cgcgatccct tctgcaatat
    21001 aagctgctaa aactggaacc atcattccca ttgctgcatc gtcaactgct tttaccattg
    21061 ccgctgtagc attgtattgt gcatgatctg gatcagaaga atagattccc caaacggcaa
    21121 atgaaatagc aatcaatact ccgccagcta ccacgaaagg caacatatga gagacaccgt
    21181 tcatcaaatt tttatagatg ctacgaccaa aattagcacc acttatcgct tctggttcta
    21241 aagtatcagc agcttcatta tctgctaaga cagtaccttt tcctgccaat gcttcgtcaa
    21301 tcagccgttg tgcatccttg attcctgtac tgacagaaac atcaatgact cgttttccca
    21361 caaatctttc tgaatggaca tctttgtctg cggcaataat gacagcatct gcctgtttga
    21421 tttcttcagc cgttaattca ttctcgatcc caatttgtcc atgggtctcc actttgatcg
    21481 tgatgccttt tttcctagct gcttgctcta atgcctcttg cgccatgtag gtgtgcgcaa
    21541 ttcctgtcgg acaaccggtt gctgcaatta gttgatattt tcccatcttt cgaacctctc
    21601 ttccttgatt ttgatttgtt tacttaattc ctccacatcc aaaaaatcag tcagtccttt
    21661 acgaaaagca gtagagctgc ctgctgctat gcttttacgt aacgtttcat caagcgatcg
    21721 tcgattcatg tatcctgcta aaaaagtgcc taacatggca tcacctgcac atgctgtatt
    21781 gacaactttt ccttttggcg agtttcctcg aatcaccctg tctttcgtaa aaagaatcgc
    21841 tcctttctca ccgagggata acaaaacatt ttctgcgcct gactctacta gtcgttgtcc
    21901 ataaacaaag taatcttcat ctgtaaccat ttttcgtccg aaccaagaag aaagttcttc
    21961 ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa tccatgactt cttgatcact
    22021 actatcaata atcaaacaaa tccgactgat ttctattaaa acctttggag acagccctct
    22081 tggtaaactc ccagaaacac aaagataatc ccctgcttgt aatgctctta tttgatttaa
    22141 aaaattatga acagctgtct gcggtatttc aggtccttga ttcactaatt tgtattcttc
    22201 tcccgtttga ttgacttgtg taaatacatt gatacgcgtc ataccaggca cttcaataaa
    22261 ctctgtttgg atttgtttct gctgtaaata gtcttctata tactttcctg tgaacccagc
    22321 agaaaaacct aatgccgtac ttgggaaatc tagcatcttt aatactagtg acacattcac
    22381 cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg tttaccataa aaggatgcat
    22441 ctcatctgtt tcaataaata gatcaattgc caaattcatc gtgcatgtgt agatagacat
    22501 aaccaacgac ctccttgatt ctattatgaa atacgcgaaa tagaaaatgt tttcttaatc
    22561 gtcccaacac aaagaaaacc tttacattta taaactcgtc gtgctttttt taactaactt
    22621 ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg aaacagagca gtacctctgg
    22681 aactttatcg aacacctcat cctagagatc cctaactatt ccatcgtgaa actaagtgaa
    22741 caagcaaatg tctcaacagc caccattgtg cggacaatga aaaagaaagg ctatgaaggg
    22801 tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca agaacatcaa tttttcattt
    22861 ttagataaag tagacaaagg aatccgccga gccattctaa aaaatgaaca agaagtggta
    22921 cgtacaatca atatgttgga aatcgggaac attgaagatg caatacaaag aatcaaattt
    22981 gctgaccggg tttttgtctt tgcccgagga ttttcagaaa tgattggcca agaaatgctg
    23041 gtaaaacttc aattaacggg aaaaaattgt cagatgcata ctgatccaga aattatcaaa
    23101 agcatcagtc ggaaactaac taagaaagac agcgttattt ttgtttcttt gaacggtgag
    23161 aaaacaaata aaaacatcaa aaataagatt tatgtcttta tttttcaatt ttttaaat
//
LOCUS       DANKEF010000040        22712 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000040 DANKEF010000000
VERSION     DANKEF010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.040"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            21..257
                     /locus_tag="PWZ48_001811"
                     /pseudo
     CDS             21..257
                     /locus_tag="PWZ48_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            472..1224
                     /locus_tag="PWZ48_001812"
     CDS             472..1224
                     /locus_tag="PWZ48_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2261468.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
     gene            complement(1840..3282)
                     /locus_tag="PWZ48_001813"
     CDS             complement(1840..3282)
                     /locus_tag="PWZ48_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
                     /protein_id="HDL2261469.1"
                     /translation="MNTIIDIANKVFTPLINLGAAPLMTIVLTVIALFFKVKPSRALE
                     GGLKLGIALTGIGAIIGILTTAFSDAMTAFVERTGISLNITDVGWAPLATITWGSPYT
                     LYFLLIMVIVNIVMLAMKKTNTLDVDIFDIWHLAIVGLFAIFCGANLFIASALVIFIG
                     VLKIWNSDLMKPTFNDLLNAPDSNPMTTTHMNYMMNPIIMVFDKIFDKIFPWLDKYDF
                     DAAKLNSKIGFWGSKFAIGIYLGIFVGLLAGQTPTQIFSLAFTAAVCLELFSLIGAWF
                     IAAVEPLSQGITDFANKRLKGRTINIGLDWPFIAGRAEIWAAANVLAPIMLLEAIILP
                     GNKLLPLGGIIAMGVTPALLVVTRGKLIRMIVIGAIELPLFLWSGTLIAPFVTQTAKA
                     VGAFPAGLNGSAMISHTTMEGPIEKFLGYLVGNASQGQIEFVIYAALALGAYLLIFIW
                     YARQMKKRNAAYAAEKEENASPATAHNRII"
     gene            complement(3304..3606)
                     /locus_tag="PWZ48_001814"
     CDS             complement(3304..3606)
                     /locus_tag="PWZ48_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDL2261470.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            complement(3663..4142)
                     /locus_tag="PWZ48_001815"
     CDS             complement(3663..4142)
                     /locus_tag="PWZ48_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2261471.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            4307..5080
                     /locus_tag="PWZ48_001816"
     CDS             4307..5080
                     /locus_tag="PWZ48_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2261472.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            5100..5528
                     /gene="lacA"
                     /locus_tag="PWZ48_001817"
     CDS             5100..5528
                     /gene="lacA"
                     /locus_tag="PWZ48_001817"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDL2261473.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTREHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            5547..6062
                     /gene="lacB"
                     /locus_tag="PWZ48_001818"
     CDS             5547..6062
                     /gene="lacB"
                     /locus_tag="PWZ48_001818"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDL2261474.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            6075..7001
                     /gene="lacC"
                     /locus_tag="PWZ48_001819"
     CDS             6075..7001
                     /gene="lacC"
                     /locus_tag="PWZ48_001819"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDL2261475.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            7014..8000
                     /gene="lacD"
                     /locus_tag="PWZ48_001820"
     CDS             7014..8000
                     /gene="lacD"
                     /locus_tag="PWZ48_001820"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2261476.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            8456..9124
                     /locus_tag="PWZ48_001821"
     CDS             8456..9124
                     /locus_tag="PWZ48_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2261477.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            9115..10248
                     /locus_tag="PWZ48_001822"
     CDS             9115..10248
                     /locus_tag="PWZ48_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2261478.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            10707..11279
                     /locus_tag="PWZ48_001823"
     CDS             10707..11279
                     /locus_tag="PWZ48_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003706982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HDL2261479.1"
                     /translation="MTENKATQERTPQERLQEEQEHKEHVHHTKINAAAIADHLLGNM
                     HTLHVKLHQYHWYVKGANFFTLHEKFEELYNENEKWFDKLAERLITSGHKPASTTVEF
                     EKYSMLSEDAVNKYAKAEDMVENIIEDFRSTRDLTIRAIRLAQDEGDDALEDTLIAFK
                     NDLDKNIWMLQAFIGKEALEDDDALDEDED"
     gene            11424..12128
                     /locus_tag="PWZ48_001824"
     CDS             11424..12128
                     /locus_tag="PWZ48_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011476586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HDL2261480.1"
                     /translation="MTKHTHHEIGDHAACIRLVPIFNHLEDSAMNYIAEKAHTKQYQK
                     GEFLFRSQEKEDTLYIINRGKIRIYRLADSGKEQLVRILNPGDFTGEWTLFNPDAVHE
                     EYAEATRDTVVCMIQQKDVQDFLEQYPAISLKLLAEMSKRLESSERQTTQVAVESVIT
                     RLVLFLAENVEPEMGNSPTITLPMAKKDIASYLGTTPETISRKFGELEDEGLIQQLSG
                     KRIKIQDLDDLLLYSE"
     gene            12357..12695
                     /locus_tag="PWZ48_001825"
     CDS             12357..12695
                     /locus_tag="PWZ48_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261481.1"
                     /translation="MHCFWVIVAITLSAAGAFFVVPFTKNTIGGEAKTSNVVEADRLA
                     MEKEEEVVKGAGVIGHGKVAYYEASDQGDKSVFTVWFPWSLAGILFSITMTRMTRKIE
                     EMEQIEWDSY"
     gene            12680..13534
                     /locus_tag="PWZ48_001826"
     CDS             12680..13534
                     /locus_tag="PWZ48_001826"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDL2261482.1"
                     /translation="MGFVLGVLNPHVVEIGSVVIHWYGVIIAAGILAALQLSTKEAKK
                     KGLEEDTIIDMALWAIPIGLLGARLYYVLFELGYYLQNPGQILAIWNGGIAIYGGLIA
                     GGGTLYWYAKKKGTSLALVLDILAPNVLLAQSIGRWGNFMNQEAHGGPVTRQFLENLY
                     LPEFIIEQMNMNGTYYHPTFLYESLWSLLGFVLIVTIRNRKQLLRQGEVALSYVLWYS
                     VGRFFIEGMRTDSLWIGEWIRVSQALSLALFIGAIVVWFIRRQDYPPISYYSDGNKGQ
                     KKTIKMVN"
     gene            13681..13914
                     /locus_tag="PWZ48_001827"
     CDS             13681..13914
                     /locus_tag="PWZ48_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HDL2261483.1"
                     /translation="MEKAILQLETLSCPSCMQKIEGAVKSVNGIDKDSIKVLFNSSKV
                     KTNFDSAVTSIEEIQKAIENVGYPVLKAKVKAV"
     gene            14112..16016
                     /locus_tag="PWZ48_001828"
     CDS             14112..16016
                     /locus_tag="PWZ48_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2261484.1"
                     /translation="MQQYILSKKNVITVISGLLIVLGFFSHFVLENVGLSEWSLIIAS
                     VFGITPIAIQAFQAMKVKVISIDVLVSIAAIGALFIQNYEESAIVTFLFLFGHYLEQR
                     TLNQTRSAIKELTEMAPESALKQMDNGEFEEVEVDDIDEGDILLVKTGAKVPVDGIVL
                     TGEGHINEASITGEAIPVNKLADAEVFAGTILENGTIQIRADRVGEDTTFGKIIELVE
                     EAQDSKSEAERFIDRFSKYYTPAVLVLAIVVWAFSQNIELAITILVLGCPGALVIGVP
                     VSNVAGIGNGARNGVLLKGSEVIQDFSNVDTIVFDKTGTLTVGNPTVAATELYEKDPA
                     ETLGYLASVERESDHPLAKAVLNQIGETNFYPVEGTEVVKGGGIVSSVAGHRVAVGNV
                     ALMEKENVTLSKKVQKDVKRFEQQGNSLVLTAVDGELKVLMGIRDQVRPGVKANLQEL
                     KDLGVKNLVVLSGDNQGTVDVVAGELGLTEAHGYMLPEDKSAYIGKLQQRGQIVAFVG
                     DGVNDSPSLALADIGIAMGSGTDVAIETSDVVLMNSNFSNLPHALGLVKATANNMKQN
                     IVISIGVVLVLLTSVFFSEWMNMSIGMLVHEGSILVVIFNGMRLMKYKLKGRKEQKEV
                     SAPAEKVKIS"
     gene            16326..16688
                     /locus_tag="PWZ48_001829"
     CDS             16326..16688
                     /locus_tag="PWZ48_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2261485.1"
                     /translation="MEKVCQVTIVHKEKVNVIKKKLDQKDFSNLLVLGKCFSDSSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVFLKLSENLSMKS"
     gene            16705..17316
                     /locus_tag="PWZ48_001830"
     CDS             16705..17316
                     /locus_tag="PWZ48_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HDL2261486.1"
                     /translation="MLQSIFSAIAVYISTSIDYLFILLIIFSQSHTKKGIRHIFWGQY
                     LGTGILVAVSLFAAYVLNFIPQDWIIGLLGLIPIFLGIRVAFVGEEEEEEEEVVEKLE
                     SRGTNRLFWTVALITIASGGDNLGIYIPYFTSLAFSEIIIALIVFAISVAVLCYISYK
                     LAKISFVSETLEKYERIIVPIMFIALGIFIMIENGTIQTLLGF"
     gene            complement(17418..18326)
                     /locus_tag="PWZ48_001831"
     CDS             complement(17418..18326)
                     /locus_tag="PWZ48_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HDL2261487.1"
                     /translation="MSKSDKDHNHSNQNQPKKNIKIAFFINLIFSISEFFFGFLFNSV
                     SIMSDAVHDLGDSISIGFAWFFQGYSEKKQDERFTFGYNRFSLLGALITGVVLISGSF
                     FMIYRSVPRLIDPQPVNYSGMFWLSLVAIALNGYAAWILSKGTSKNESMLNLHMLEDV
                     LGWIGVLIVSILMNFTEAYRLDPILSILIALYILYKTVPEFFTTMKILLNGSPENVNV
                     KSLADSIKNVPAVKDLSHFHIWSLDGEENALIITVLIDPSDIDKAREIKEEIAEVAHK
                     SSVENHITIEITTSKEELEKGYSHNT"
     gene            complement(18368..18682)
                     /locus_tag="PWZ48_001832"
     CDS             complement(18368..18682)
                     /locus_tag="PWZ48_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDL2261488.1"
                     /translation="MDIKTTSPIDKGSILSISKLFKVISDPTRLSVLFLLQKKECSVG
                     NIAISLDMEQSAISHQLKTLKDARLVKSRREGKSMLYSLDDLHVFSILEQVLTHVNEL
                     EK"
     gene            18981..20912
                     /locus_tag="PWZ48_001833"
     CDS             18981..20912
                     /locus_tag="PWZ48_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HDL2261489.1"
                     /translation="METIQEQQSQEKHNHNHNHDHDHDHGKMPVVLYFVGLALAVIAL
                     FLNEDYQLVQNVLFSIATISAGYHVILLEGLGETIENSKAQKKFIPNSHILMGLAAIG
                     ASLMGNFWEGTLLILIFSGAHFLEDYAEGRSKREITKLLEMNPTTARLILPDGSTKNV
                     DVSELKVGDQLQVLNGDQVPIDGVILSGSTSIDESSITGESIPKEKSKGDGVFGSTIN
                     GTGTFTMKVTKENKDTVFSKILQLVNQNQDNQTKAASIIQKFEPKYVTVVLIAIPLFM
                     LLAPFLLDWTWSQSVYRGLVLLVAASPCALAAATVSATLSTTSNLAKKGVLSKGSSYL
                     SQLADIKAIAFDKTGTLTKGKPEVTNYYFADSVNEENMIDIVVALEKESNHPLADAIL
                     RKFEQKNKLTIEVENQIGKGLTGDYKGRNYRIGKPTSFDDVSDEYSRLNNEWASEGKT
                     VVYVAVDEEVIGLIALMDVPSEYAKETIEYFRKQGIHTTLITGDSEMTGKAVAKQLGI
                     DEVIANVMPEDKSRIIDEQKEKYGVTAMVGDGVNDAPALVNADVGIAMGDGTDVAVEV
                     SDLVLMQNNLSKLVQSHNISSKMNRVIWQNIIFSMAVVAFLIVVSFLGLTDIAISVII
                     HEGSTLVVILNGLRLLKAV"
     gene            21205..21756
                     /locus_tag="PWZ48_001834"
     CDS             21205..21756
                     /locus_tag="PWZ48_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2261490.1"
                     /translation="MGYNVQPLRSQQEINDFLFCLRRNKNAERDVFLFLIGINSGLRM
                     SDIVKLKKKDVITSKNPRIVEQKTGKTRILYLSSLQDLIHGYTAPLDPEAYLFPSTKG
                     GHLEVNTVYQMFQKVAKLLGRDDIGTHTLRKTFGYHYYKKTKDVATLMEIFGHSSEKI
                     TKRYIGINEDEISETLLNFRLGF"
     gene            22069..22611
                     /locus_tag="PWZ48_001835"
     CDS             22069..22611
                     /locus_tag="PWZ48_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261491.1"
                     /translation="MSIKETTQITRQLNAIYKAAHLLQEHFVDKKVSFVGEVSTVAII
                     FSTTNFMHLCGIDYRRGTHLFFQDALDRKINLQDIQIKTDGTTFQKLQVIGSLDLLLG
                     KHISIVGRGVYSSLRYDAAIRTRKKILALSLKQNGLIYIPISLLNLSSKEIGPGQKVT
                     GIFSEDLTSGELKMIMEVID"
BASE COUNT         7394 a         3819 c         4853 g         6646 t
ORIGIN      
        1 gcttaatttg acaaatcaga aaagaaaaaa tgtatcaagt ttttgaagaa atgggttatc
       61 caattaaaac ggtcaccaga aaactagaag taatcaaagc ggatgatacg atcagcgatt
      121 tgttagatgt ggaagaagga gatccaatgt tctatttcca tacgcagggt ttttcgctag
      181 aacgtctgcc cgttgagtat tcgatttcga aatatcgagg agacattaac tactttgttt
      241 ttgaacttgc acaataactt cagattaaat aaaatgattt attaaagata aaagtcgaat
      301 gaacaaggct gtcactatga cagtcatgct cgttcggttt tattttttag aatagcagct
      361 tgcaaattct gaaaaatttt gctttccttc ctgcagaagg cttcttaaga aaacttgttc
      421 tagcactttt cttaagaatt tgatagtctt aaaaagaaaa gaggcgatga aatgttaaaa
      481 acggatcgat tccaagcgat catgtcgtta ttagaggtta acggtacagt acgagtcagc
      541 gatattatgg agaagttttc ggtttcggat atgacagtcc gacgtgattt ggacgaaatg
      601 gagaaacaag gattattaga acgcgtccat ggcggagcga aattgaagga tttttatcgg
      661 catgtggagc tttctcatag agaaaaacag attatgctgc aagaagaaaa gcagcggatt
      721 gtggaaaagg cgaatgaaat gatcaatgat ggtgatacga tatttcttgg accaggaact
      781 acaatcgagc tcatgtcaaa aatcattcaa ggggattcac tgcggattgt tacaaactgt
      841 ttgcctgttt ttgaagaatt aaataagcga aaagggaatc gaaaaatcta tctattaggt
      901 ggcgagatgc gggaaacgac tcaatgcttt gttggtgaaa tcacgaataa atcgctacag
      961 gatatgaatt ttcataaagc atttttcagc tgtaatgctt taaatgaaca gaaaattatg
     1021 acttcgactt ttgaagaggg ccaaactcaa cagatcgctc tgaataattc tgttgaacgc
     1081 tttttattga ttgatcattc gaaaattggg aaaagagatt tttcagtata ttataatctg
     1141 aatgatgtaa cagtagttat aacggatgat gatgaacaaa agcactatga aaaaatcgaa
     1201 aaagaagaaa aatatgttgt gtaaatgaga aactgccaaa ctctagataa ttagaacttg
     1261 gtagtttttt ggtagccttg aatttaagcg aattatgtta tttttagggt gcatcattac
     1321 taattcctcc gaatacttga atacaccatt tgaaaaaaga tagtcgattt ttttgggatg
     1381 ttatgtttat gtgaaggaaa aaatagcagt atcaatcacc gctttattaa ggaggaaaag
     1441 ggatatcaaa acaaccatgg acactaagtc agctgaagct ctttgacatg aaacgagtta
     1501 gatcagaaaa agcaatgatg aaatcgcacg cataaccgcg ggatgcgaag cgattcagga
     1561 aaaaagaaat caaggaattc gcaattaaac aacaaaaagt caaaaagcac aaaacaaaca
     1621 agaacacaaa aagaatcgtt gaggaatacc aacaaaggca agcttaaagg agttaaaaaa
     1681 agcataaacg ttgataattt ctttattggg tgtggcaaga gatgggctat gctttttgaa
     1741 atggatttta catctattta ttttttatag gtaaaataat accccgaagt ctaaagacat
     1801 tcggggtgtt tttagcttat ttagcttcgg ctgcataagt catattatgc gattatgcgc
     1861 cgttgccgga gatgcattct cttctttttc agctgcgtat gcagcgttgc gttttttcat
     1921 ttgacgagcg taccaaatga agattaaaag gtatgcacct aatgctaagg cagcgtatat
     1981 cacaaattcg atttgtcctt gagaagcatt accgactaaa taacctagga atttttcaat
     2041 cgggccttcc atcgttgtat gagagatcat ggcggacccg ttcaagccgg ctggaaaggc
     2101 tccgactgct ttagctgttt gagtaacgaa cggggcgatc aaggtgccgg accataagaa
     2161 taatggtaat tcgatcgcgc cgattacgat catacggatt aattttccac gagttacgac
     2221 caatagtgct ggggtaacac ccattgcgat gattccgcct agtggcaata atttatttcc
     2281 tggtaagatg atggcttcta gcaacatgat tggtgctaat acgttagcgg cagcccagat
     2341 ttcagcgcgg cctgctatga aaggccagtc taaacctatg ttgatggtac gtccttttag
     2401 tcgtttattg gcaaaatcgg taatcccttg tgataacggt tctacggctg cgatgaacca
     2461 agcgcctatc aaagagaaca attccaaaca gacggcagcg gtaaaggcta gcgagaaaat
     2521 ttgcgttggt gtttgtcctg ctagtaatcc aacaaaaatc cccaaataaa ttccaatcgc
     2581 gaattttgat ccccagaagc caattttact attcaatttg gcagcatcga aatcatattt
     2641 atccaaccaa gggaagattt tgtcgaaaat tttatcgaaa accataataa tcggattcat
     2701 catgtagttc atatgagtcg tggtcatcgg gttgctgtct ggcgcattta acaagtcatt
     2761 gaaggttggt ttcattaagt cggagttcca aatttttaat acaccaatga aaataactaa
     2821 tgcagaagca ataaacagat tagcaccgca gaagatcgcg aacaaaccaa caattgctaa
     2881 atgccaaata tcgaaaatat ccacatctaa tgtgttggtt tttttcattg ctaacatgac
     2941 aatattgacg atcaccataa ttagtaaaaa ataaagcgtg tatggcgacc cccatgtgat
     3001 ggttgctagt ggggcccaac cgacatctgt aatgtttaat gaaatacctg tgcgttcaac
     3061 aaaagctgtc atagcgtctg aaaaagcagt agttaaaatt ccaataatag ctccgatccc
     3121 agtcagggcg atccctaatt tgagcccgcc ttcaagagcg cgtgacggtt ttactttaaa
     3181 aaataaagca ataacagtta atacgatggt cattaacggt gccgcaccta aattaattag
     3241 gggggtaaat actttattgg caatatcaat aattgtattc atatgctttt cctttcttta
     3301 gtcctatttg tttaatgatt ggattgcgct ttccatactt tggtaaacag gctcagccat
     3361 agcaggaata cgataaagaa tagcacctgc atcaacgaca gggatgttaa tgtcaaaacc
     3421 caagtcggtg ttggcaattt gtgcgaaaat gtcataatga gacagcattt cttcattgat
     3481 atctttgatc ataacggcgt cacaggtgac ttgatagcca cgatttttca attcagtttc
     3541 caaagcgctt ttgatttgat ggcttgaatt aacgcctgct ccacatgctg ctaaaatttt
     3601 gatcataata attctcccct taaaataagt ttatttttta tcctgcggtt aggcagagac
     3661 ttctatatat aaaaatgttc ttctaaaaaa tgataaatcg cttctgtgtc ctctaatttg
     3721 aaaaacgaaa acagtttttc ttcgtcaaca gaattaatga aatccatgat attagccaac
     3781 atgccagcct gtgcttcacc attttcgttc aagatcatga aaagaaatga agcggaaatc
     3841 tgatcgcttg gcgagatcat gttgtagaaa gggatttcat tttttacttt gattggaatg
     3901 atacgggtgg ttcgcacaaa ctcactttcg gtatgaggga tagcgatatc tggcaatatt
     3961 ttagagacag gtgagagatc taatcccgtc ggataattac gctcccgttc taaaaggttc
     4021 tccaaaaaat cttcggttac caattctttc tttaacagat cttgatagac ttcctcgaac
     4081 acttcttcct gcgactgctt gtcagaaaca tagacagtgt ctttgaaaaa gaattcattc
     4141 acacaaaaca gctcctttat tagttcgttt atgtttattt atctgtttac aagttgagta
     4201 taaccgctta caaaattatt gtcaacgcat aaacgaaaat aaacgaacaa tatgtgttga
     4261 aaaatgttcg taaatgtttt attataaggt cgtggaggta cagaatatgc tgaaaacaga
     4321 acgacagcgt tcgatcttag cgatgtgtga tcgctatggg acgataacag tcaaatttat
     4381 tcaggaagta ctgaatgttt cagatatgac gatccgccga gatctagagg aactcgcgca
     4441 gaagaaaaag cttttacgcg ttcatggcgg tgcacaaaaa atcgattatc tggaaatgtc
     4501 gacttctata gaagcaaatg agctttctca tactgaaaaa aagaagctgc atacaaaaga
     4561 gaaggaatac atcgctaaaa atgcggcagc tcatatcaat caagaagacg acacgatttt
     4621 tcttggatcg ggtacaacga tcgaattaat gacacgatac atcaagccgt catctctccg
     4681 aatcgttacc aatagtctgc cggttttcaa tctattaagt gaaataaaaa gatttgaact
     4741 gtatttgatc ggtgggctat atcgggaaaa gactggagcc tttgtcggtt caattgccaa
     4801 tgaagctgtt caaaaaatcg gtattaagaa ggcttttgtc ggagtgaatg gattgaaggg
     4861 ttcggaggtt tcgaatttca gtattgaaga aggtaccttg caaaaaattg ttttagataa
     4921 tgcggcagaa aaatgtttgg tttcagatgc aaacaaattc gaccatagcg atttttataa
     4981 tttctatgat ttgcgcaaga tcgacaaatt atttacggat tctactcttt cagccgagct
     5041 gaagagttat tatcagcaat tcactaaaat cattcagtaa aaaataggag gaaacaaaaa
     5101 tgcgtgtagt aattggttca gacagtgacg gcatggaatt aaaggaatta atcaagaaaa
     5161 aattgatcaa agaaggtatt acagtagtag ataagagtga aacggcttca gaagattttg
     5221 tggaatcaac tttggcgatc gcaaatgatc tgaatgaaaa cgaaggtagt ttaggaatcg
     5281 cggttgatgg ctacggcgct ggcagcttta tcacggcaac aaaggttaaa ggaatggtag
     5341 cagcagaagt ttcggatgag cgcagtgcgt atatgacccg tgaacataat aattctcgga
     5401 tgatcacact tggatcaaaa attgttggcg atgagttggc gttgaatatt gttaaggaat
     5461 ttctacaagc aaattatgat ggcggtcgtc atcaagtacg tgtcgacatg ttaaataaaa
     5521 tggcgtaatt aggaaggatg aatgaaatga aaatcgcaat tggctgtgac cacatcgtca
     5581 cagatacaaa aatggcagta tcagatttct taaaatcaaa aggacatgaa gtgttggata
     5641 tggggaccta tgactttacc agaacccatt atccgatcta cggaaaaaaa gtcggagagg
     5701 ccgtgactag cggagaagca gatctaggag tttgtttgtg tggaacaggt gttgggatta
     5761 acaacgctgt caataaagta cctggtatcc gcagtgcgct ggttcgcgat atgacgactg
     5821 ctatctatgc gaaggaacaa ctaaatgcca atgtgatcgg ctttggcgct cggattacgg
     5881 gagaattttt gatctgtgac atattagagg ccttcatcaa tgccgattat caagaaactg
     5941 aagaaaacaa aaaattgatc gccaaaatcg atcacgtcga aaaagccagt gacaagcagt
     6001 ctgatccgca tttcttcgat gaatttatcg agaaatggga tcgtggcgaa taccacgatt
     6061 aagcgggtgg acaaatgatt ttaacaataa cgatgaatcc atcgattgat atttcttatc
     6121 cattagaaac cttcaagctc gacacagtca atcgagtaag aaatgttcgc aaaacagctg
     6181 gcggtaaggg cctaaatgtg acacgtatct tgaagcaatt agatacggag gtgattgcta
     6241 gtggtttaat cggcggtttt ttaggtgaag agattaaaaa ggacctgact caaaatgaga
     6301 ttgctcatga ctttttacct atcagcggag agacacgtaa ttgcatcgct gttttacatg
     6361 acggaaaaca gacggaaatc ctagaaaacg gacctgttat tactgaacaa gaagcaaggg
     6421 cgttcttgca acatttcgaa aaattagctg ttaaagccga aatcatttct ttttcaggaa
     6481 gcttgccaga aggattgcct gctgattttt atcgacaaat gctgcaagtt tgtcagccaa
     6541 agcctgtcgt tttagattgt tcaggtacag cattaaggga agtgttgcga ggcagtgtaa
     6601 agcctaagct aataaaaccc aataccgaag agctggctgg tctgctagga cgagaaatct
     6661 caacagaatt atcagattta aaagccgcct tgtcagattc gatgtttgat gaaattgaat
     6721 ggatagtggt ttcaatgggg ggcgacggag cctttgccaa acatcacgag actttttatt
     6781 gcgtgacaat tccaaagatt gatgtcgtca atccagttgg ctctggtgat gcgaccgttg
     6841 ccggattgac cgctgcgatt caacaaggtc tttcggatga agccgtgtta aaacagggta
     6901 atgttttagg aatgctgaat gctcaggaag cgacgactgg ctatgtccaa ttagccaatt
     6961 atcaaaaact atatgatcaa atcttagtgg agaaagtgta ggaggatacg gaaatgaacg
     7021 aagaaaaacg tcaatatatc aagcaactat gcaatgaaga tggagtgatt ggggccttag
     7081 caattgatca acgcggggct ttgaagaaga tgatcacaaa attcaagggc agcgaagcag
     7141 aagaatcaga aatcgttgaa tttaaagaaa ttgtttcttc acaattaacg aaatatgcct
     7201 catcgatttt attagatccc gaatatggct tgccagcagc tgacgttaga gcaaaaaatg
     7261 ccggcttgct gttagcttat gaaaaaactg gttacgatgc gtcaactccc ggccgtttac
     7321 cagatttatt atcgatctgg tcagttaaac gtttaaaaga agcaggcgcc gatgcttgta
     7381 agttcctact ttactatgat gtcgatgaag cagaagaaat caataatcaa aaacatgcct
     7441 ttattgaacg aatcggctca gaatgtcagg ctgaaaactt acccttcttc ttggagcttg
     7501 tttcttatga cgcgaacatc tctgatgcga cttcgaaaga atacgccatt aagaaacctc
     7561 ataaagtcaa cgatatgatg aaggaatttt ctgatccacg ttatggcgta gatgtgttaa
     7621 aggtggaagt acccgtcaat atgaattatg tggaaggcta tgggacagac gaagtcgtgt
     7681 acacaaaggc acaagcggct aacttcttca aggaacaggc tgaagccact aacctgccat
     7741 ttattttcct aagcgccggt gtaaaagcag agttgttcca agaaacactg cgcttcgcga
     7801 aaaatgccgg ctcaaccttc aatggtgtac tttgcggccg tgcgacttgg gcagatggtg
     7861 tagaaagcta tatcgttgat ggaaaagaag cagcgatgga gtggatgaat acgcaagggc
     7921 gtaaaaatat tgaagaatta aatgccgtat tgaaagaaac tgctacaccg gtggaagtgt
     7981 taacaagctc actatcataa gaaatcagac tgtgaagcac aattgcttgc agtctttttt
     8041 tatacataaa gcgtaaaatc atgctgcatt attaggggaa cgaattctaa aatagtaagt
     8101 gagttaaaac gaaggggttt ttctttagag agattttctt acatgtggta cttaatgact
     8161 tgatggcaat tttcagtatt ctaacgaggc ttattggtca taaaccaacg aactttgaaa
     8221 agtatataca agaaaaccaa gcaaatagaa agtagtgaga ctggaggaaa ggttccagtc
     8281 tttttctatt ctaggtattt tcaaacagta aaatcattct taattatgtg ttttttaaca
     8341 gaatgtatga atttcttcaa atcttcacaa aaagaacgtg gactttgaag aagagcgtgt
     8401 actatgctaa aaagcataag tgtaattact aaatccgtat gaggagtgaa tatacatgcg
     8461 tgtactggtg gttgaggaca atcgtgagct ggttttatcc gtaaaaaagg gcctagagca
     8521 ggagaatatt tctgtagatg tagcttatga tggcgagtca ggagaagaga aggccttcat
     8581 caattcatac gatgggatac tactagattt aaatctgcct aaaaaagatg gattatctgt
     8641 tttacaaagt ctccgtgctg aagaagtcga aagtccagtt atcattatta ctgcacgtga
     8701 tgagattgaa gaacgagcaa aaggcttgga ttttggagca gatgattatt tagtcaaacc
     8761 ctttgagtta ctagaattaa aagcacgaat acgcgcggtt attcgtcgtt atcatggacg
     8821 tgcgctgccg cagatttcaa ttggtgcttt gaagattgat ccattaggtc gaagggtcag
     8881 ctatcaaaag acagaggtgg tcctgaaacc caaggaattt gatatcttac tgtgcatcgc
     8941 tcagcgtcat ccagcggttg tttcgtctga ggaaatttct gaacatgtct acgatgaaga
     9001 ttttgatcct ttttcgtcgg ttctgcgagt gcatctggct cgtctaaaaa agaaactggc
     9061 tgaagcagct gaaaaagagg tcttaaaaac cattcgagca aaggggtacc agctatgcta
     9121 ttaaatttta aaatgacgtt aacgatgctg gctttttcac tgttggtgac aggcgtgatt
     9181 ttagtgagtt tcttctttct gagaaataat ttggagggaa tcagtatcgc atctacctta
     9241 ggtgaagatg tttattttat ggaagagggt aagacggttc aagcagagac gcagatggaa
     9301 gcttcgaaat cttccaacaa tcaatttatg actatttcga tgatagataa agcttaccag
     9361 gacgaactat ttaaatattt accagcgatg gtgctggtta tttgttctgc cgtcatgtta
     9421 ttaacgttta ttctttggtt ggttttacgc aaaatttaca gtaatcaaat cttagcgatt
     9481 acgcgtgatt taacgtttat tgatcaagtt cctaaaaata ctgtgaagga cgaggcatta
     9541 aatgcggctt ttaataagct aaaggataag ttcgatggtc atttggagga ttatcgacga
     9601 ttaaatagtt atctgaccca cgagcagaaa aatgccatcg ctattttgcg tacgaatctg
     9661 gaaatagaac ggcaacatgc cgatcaagga aatttaatgc ttttagatcg gatgaccgac
     9721 agtattgacg atattttgac attatccaat aactcagagg atcttagcac ggaagtcgta
     9781 gacgtttcac tgatctgtgc ggaagtttgt gatgcctaca aaatgaatta tccgaatctt
     9841 ttgtttgcgt ttactgaaga tcaaccaacc ctgattcatg gacgagatcg ttggatttat
     9901 cgggcggttt cgaatttagt tgacaatgca atcaaatatg gtgaaggaaa actggtaatt
     9961 gtcaccattt caaaccaaaa gggtagcgtg attattgaag taaaagatca gggaattggc
    10021 attcctcagg agaaacaggc atcaattttt tctcatcagt accgagtgca tgggctcaag
    10081 caggatggct tacctaaaac aatggacttt agggacacgg atgaaacctt tattcaatcc
    10141 gttgcctcga aaagaaacca tattccaaga aaatcattaa attatcgaac accattggaa
    10201 gtatttttga gctgcataga taaagacata ttgtctagct taatttgaca aatcagaaaa
    10261 taaaaaaatt cttttcaatt tttatttatg gtgtgacata aataaagtaa agttattctt
    10321 ctaggttaat cttattaatg ctctttctca ctagtgttgc atgcaacact agtgagaaag
    10381 agtattaaat atactgcaag gtaagacatt tggtaattga agaataacaa gcaaaaaaat
    10441 agcagtcagg tctcttgaca aagtatagtt gctattcttt ttttaggccc tactttaatg
    10501 aaatttttgt ttggagggga cgacttcatt cctttgatta tttcagttga atttaataga
    10561 gaagcctggc gtctattaag aaatactttt cttgatgagc gtcaattttt ttaacggaaa
    10621 tctatcctat actaaattca gtaaagaaaa aggaggaatc aaagaaagct attttaaaca
    10681 aaccaaaatt ataggaggaa taaatcatga ctgaaaataa agctactcaa gaaagaacac
    10741 cccaagaaag attgcaagaa gaacaagaac acaaggaaca tgttcatcat acgaaaatta
    10801 atgcagcggc cattgcggat catcttttag gcaacatgca tacattacat gtgaaactgc
    10861 accaatatca ctggtatgta aaaggagcca atttctttac gttgcatgaa aaatttgaag
    10921 agttgtataa cgaaaatgaa aaatggttcg acaaacttgc ggagcgtcta attacttctg
    10981 ggcataaacc tgcttcgaca acggttgaat ttgaaaaata ttcgatgctt tcggaagatg
    11041 cagttaataa atacgctaaa gcagaagaca tggttgaaaa tattatcgag gacttcagaa
    11101 gcactcgtga cctaactatt cgcgcgattc gtttagctca agacgagggt gacgatgctt
    11161 tagaagatac attgattgct tttaaaaatg acttagacaa gaacatttgg atgctgcaag
    11221 cgttcattgg taaagaagca ttagaagatg atgacgctct tgatgaggat gaagattaag
    11281 gtaaaagtag aagctgattt agggattgga attctttcca gtcctttttc cattttcttg
    11341 atgtgcatca atttttttgt agtaagagac ttatataata aaagcagcga atataaacaa
    11401 aataaataaa gggggagaat gaattgacta aacataccca tcatgaaatt ggcgaccacg
    11461 cagcctgtat ccgcttggtt ccaatattca atcacttaga ggatagcgcg atgaattaca
    11521 ttgctgaaaa agcccacacg aaacagtatc aaaaaggaga gtttctgttt cgttcacaag
    11581 aaaaagagga tactttatat attatcaatc gtgggaaaat ccgtatttat cgattggcag
    11641 actctggcaa agagcagcta gtgcggatct tgaaccctgg tgatttcacc ggagaatgga
    11701 ccttgttcaa cccggatgca gtacacgagg aatatgccga agcaacccga gataccgttg
    11761 tctgtatgat tcagcaaaaa gatgtccaag attttctgga acaataccca gctatttctt
    11821 taaaactatt ggcggaaatg tctaaacggt tagaaagttc agaacgtcaa acgacacaag
    11881 tagccgtgga gagtgtcatt acccgtttag ttttgttttt ggcagaaaat gtggaacctg
    11941 aaatgggcaa ctctcccacc atcaccctgc cgatggcaaa aaaggacatt gcttcctatt
    12001 taggaacgac acctgagacc atcagtcgca aatttggaga attggaggac gagggattga
    12061 ttcaacagct gtctgggaaa aggatcaaga ttcaggattt agatgattta ttgctttaca
    12121 gcgaataatc ggacacactt ttttctacac gttggtgttc tgtcgctctt cattaacaac
    12181 gggacagaac tcagtgcttt tgcttccatc tgtccgtttt tgactttttt aagacagaac
    12241 aaccttcatt cattggaaaa ataaaagcac aaacatgata accctcaagt ttttttgttt
    12301 tatttttctt taagataggg ttgtcgaaca aaacgtaaaa ataagtaggg gtgaatgtgc
    12361 attgtttttg ggtgatagtt gccattacgt tgtcagcagc gggtgcattc tttgtcgtcc
    12421 ctttcaccaa gaacacgatt ggcggcgaag caaaaacgtc taacgttgtt gaggcagata
    12481 ggttagctat ggaaaaagaa gaggaagttg taaaaggagc cggcgtaatc ggacacggaa
    12541 aagtggcgta ttacgaagcg agtgaccagg gagataaaag tgtattcact gtttggtttc
    12601 catggagctt agccggaatc ttgttcagca tcaccatgac tagaatgacg cgaaaaattg
    12661 aagaaatgga gcaaatagaa tgggattcgt attaggcgtt ttgaatcccc atgtggtgga
    12721 gattggttct gttgtgattc attggtatgg agtcattatt gcagcgggca tattagcggc
    12781 tctccagttg agcaccaagg aagcaaagaa aaaaggactg gaagaggata ccattatcga
    12841 tatggcctta tgggcaattc cgattggact cctcggcgct cggctttatt atgtgttatt
    12901 tgaacttggt tattatctgc aaaatccagg acaaatcctt gcgatttgga atggcggcat
    12961 tgcgatttat gggggattga ttgcaggagg gggtacgcta tattggtatg caaagaaaaa
    13021 aggtacttct ttggcactcg ttcttgatat tttagcgccc aatgtattgt tagcccagtc
    13081 cattggtcgt tggggcaatt ttatgaatca ggaagctcat ggtgggccgg ttactcgtca
    13141 attcttggaa aatctttatt tacctgaatt tatcattgaa caaatgaata tgaatggtac
    13201 ctactaccat ccgacttttt tatacgagtc attgtggagt ttgttgggct ttgtcctcat
    13261 cgtcactata cggaaccgaa aacagctttt gcgtcaagga gaagtcgctt tgagttacgt
    13321 tttgtggtac tcagtgggtc ggttcttcat tgaaggtatg cgaacggaca gtttatggat
    13381 tggcgagtgg atccgcgttt cgcaagcctt gtctctggcc ctgtttattg gcgcgattgt
    13441 agtatggttt atacgcagac aagattatcc accaatttct tattattctg atggcaataa
    13501 ggggcagaaa aagactatta agatggtgaa ttgagcaaaa agataaaaag aagtgcaaaa
    13561 cttgatatgc gtcaattttc gtttgctaaa agtgagctat actacagttg taaagaggaa
    13621 aaggcagtgt tcattaatct gctgccgata aaataaaaac acataaagga gataggaatt
    13681 atggaaaaag ctatattgca attagaaacg ttgtcttgtc caagttgtat gcaaaaaatt
    13741 gaaggtgccg tgaaatcagt taacggtatt gataaagaca gcattaaagt attattcaac
    13801 tccagcaaag tcaaaaccaa ttttgattca gctgtcactt cgattgaaga gattcaaaaa
    13861 gctatcgaaa acgtaggcta tccggttctt aaagcaaaag tcaaggccgt ttaacagatt
    13921 aaatgaacta ggcaattctg gaagaaaagc tatttttcca ggattgcttt ttttgttgca
    13981 atccgattcg aggtgcttag aaacgataaa agagaacttt ctaaacttga tgtcagtcaa
    14041 ttaattactt aggcaattat cttatactgt gtttatcaat aaaagagaaa ttaaatttgg
    14101 agggaaaaag catgcaacaa tatatattaa gcaagaaaaa tgttatcacc gtcatcagtg
    14161 gattgttaat cgtactcggt ttcttcagtc actttgtttt agaaaacgta ggactttcag
    14221 agtggtcttt gatcatcgcc tctgtctttg ggattacgcc aattgccatt caagcgtttc
    14281 aagcaatgaa agtcaaagtc atcagtattg atgtcttagt cagtattgca gcgattggtg
    14341 cgctttttat tcaaaattat gaagaatcgg ctattgtcac gttcttattc ttattcggac
    14401 actacttgga acaacgaaca ttgaaccaaa cgcgatctgc tatcaaagaa ttgactgaaa
    14461 tggcacccga aagcgccttg aaacaaatgg ataatggcga atttgaagaa gtagaagtgg
    14521 atgatataga tgaaggggat atcttgctcg ttaaaacggg tgcgaaagtt ccagtagatg
    14581 gcatagtcct tacgggtgaa gggcatatca atgaagctag cattacaggt gaagctattc
    14641 cggtcaacaa gctagctgat gcagaagttt ttgcaggaac cattttagaa aacggaacga
    14701 ttcaaatcag agctgaccga gttggtgagg acaccacatt tggaaaaatt attgaactcg
    14761 tggaagaagc acaagattct aaatccgaag cggaacggtt cattgatcgc ttttctaaat
    14821 actacacacc agctgtcttg gttctggcaa ttgttgtatg ggcgttcagt caaaatattg
    14881 agttagcaat caccatcttg gttctaggtt gcccaggcgc attagttatc ggagtgcctg
    14941 tctcaaacgt tgccggtatt gggaatggtg ctcgtaacgg tgttctttta aaagggagtg
    15001 aagtcattca agacttcagt aatgtggata caattgtatt tgataagaca ggtactttaa
    15061 ctgtcggaaa cccaaccgtt gcagcgactg aactgtatga aaaagatcct gctgaaacgc
    15121 ttggctatct ggccagtgtg gaacgggaat cagatcatcc attagcaaaa gcggtcttaa
    15181 atcaaattgg agaaacaaac ttttatcctg ttgaaggaac tgaagttgtg aaaggcggcg
    15241 gaatcgtttc aagtgtagct ggacatagag tggctgttgg gaatgtggcc ttgatggaaa
    15301 aagaaaatgt cactctcagc aaaaaagtgc aaaaagatgt caaacggttt gaacaacaag
    15361 ggaactctct cgttttgaca gcggtcgacg gtgaattaaa agtactgatg ggcattcgcg
    15421 atcaagtccg tccgggtgtg aaagctaatt tgcaggaatt aaaagacttg ggcgtgaaaa
    15481 atctagtcgt tctttcagga gataaccaag gaaccgttga tgtggtcgcc ggcgaacttg
    15541 gtttgaccga agctcacggc tacatgttgc cggaagacaa atcggcttat atcggaaaac
    15601 ttcaacaacg tggtcaaatt gtagcctttg ttggagatgg cgttaacgat agtccgtcct
    15661 tagctttagc agacattgga atcgcaatgg gaagcggaac ggacgtagcg attgaaacat
    15721 ccgatgtcgt tttaatgaac tcgaacttca gcaacttgcc tcacgcatta ggattagtaa
    15781 aagccaccgc aaataatatg aaacaaaata tcgtgatttc aattggagta gtgttggtct
    15841 tgttgaccag cgtcttcttc agcgaatgga tgaatatgtc tatcggaatg ttggttcatg
    15901 aaggtagtat cttggtggta attttcaatg gcatgagatt aatgaagtat aagttgaaag
    15961 gtcgaaaaga acaaaaagaa gtatcagcac ctgcagagaa agtaaaaata tcttaagaat
    16021 aaattagaag aggctgagac aatagtccat ccttgaaatg acaaaagcgg aaactcagat
    16081 ttttaaatct gggattttcc gcttttttga gttcttgaaa aaaaataaga gtatagagta
    16141 tgttctttat aaaattaatt ttcgtgtaaa tgattaaaaa atttaataat aaaatttaaa
    16201 ttcctattaa gttgtaacac atgaaattgc ttataatagt gtattgaaat taaggtagat
    16261 tgacaaacga gtgatcaaat agatatacta ccttcaaacg accgtttgaa tgtgaggtga
    16321 gatgaatgga aaaagtttgc caagtgacta tcgtacataa agaaaaagta aatgtaataa
    16381 aaaaaaaatt ggatcaaaaa gatttttcaa acttacttgt gttagggaaa tgtttcagcg
    16441 attcttctag gattaaaatt ttctatgctt tagaaaccta taaggaaatg tgtgtctgtg
    16501 atttagcaga aatacttact gctagtgtcg ctacaacgtc acaccattta cgttttttaa
    16561 aaaaacatgg aatggcaaaa tcacgtcaag atggaaaagt agtttattat tctttagcga
    16621 atgaagacat cctttccgct attcgtgttt ttttaaagtt atcagaaaat ctatctatga
    16681 aatcttagtt tggaggaagt tattttgtta caaagtattt tttcagctat cgctgtttat
    16741 atttctacca gtattgatta tttgtttatc ttgctgatta tcttttctca aagccacaca
    16801 aaaaaaggta ttcgtcacat tttttggggt caatatcttg gaacaggtat tttggtagcc
    16861 gtaagtttgt ttgctgctta tgtgctaaat tttattccgc aagattggat cattggtctt
    16921 ctcggtctaa ttccgatttt cttaggaatc agagtcgctt ttgttggcga agaggaggaa
    16981 gaagaagagg aagtcgttga aaaactcgaa tctagaggaa ctaatcgctt attctggaca
    17041 gttgccttaa taacgattgc ttctggaggc gataatttag gtatttatat tccatacttt
    17101 acctcgttag ctttttcaga aatcattatt gctttaatcg tattcgctat ctctgttgcg
    17161 gttctctgct atatcagtta caaattagcc aagatttctt ttgtttccga aacgttagaa
    17221 aaatacgaac gaatcattgt ccctataatg tttattgcgt taggaatttt tattatgata
    17281 gagaacggga cgatacaaac gctactaggt ttttaaaaat cataatcacg aaagataaaa
    17341 agagaggcag cgactaatca aaatcgctgc cttttttgct tttcaaataa tgaagtattt
    17401 tttagcgtag aactatctta cgtgttgtgg gagtatccct tttcaagctc ttctttgctt
    17461 gtagttattt ctattgtgat atgattctct acgcttgatt tgtgtgcgac ttctgcaatt
    17521 tcttctttta tttccctagc cttgtctatg tctgaagggt ctataagaac cgtgatgata
    17581 agggcgtttt cttccccgtc cagtgaccaa atgtgaaaat gtgagaggtc ttttacagca
    17641 ggaacatttt taatggagtc cgccaagctc tttacgttta cattttcagg cgaaccattc
    17701 aataaaattt tcattgtggt gaaaaattca ggaacggttt tgtagaggat ataaagtgca
    17761 atcaaaatcg ataagatagg atccagacga tatgcctcgg taaagttcat tagaatactc
    17821 acaatcaaga ctccaatcca ccctaatacg tcttccagca tatggaggtt caacatgctc
    17881 tcgtttttgg atgtgccttt gcttaaaatc cacgcagcat atccattcaa tgcaatagct
    17941 acgagagaaa gccagaacat gccactataa tttacgggtt gtgggtcaat caaacgtggc
    18001 acactacgat agatcataaa aaacgagcca ctaattagca caacgccagt tatcagcgcg
    18061 cccagtaaag aaaagcggtt atagccaaaa gtgaaccgtt catcttgttt tttctctgaa
    18121 taaccttgaa aaaaccaagc gaatccaatt gaaatagagt cccctaaatc atgaactgcg
    18181 tcggacataa tggacacact attgaaaaga aagccaaaga aaaattcgct tatagagaaa
    18241 ataagattta tgaagaaagc aattttaata ttctttttcg gttgattctg attcgagtga
    18301 ttatggtctt tatctgattt tgacatttaa aaacctccta agtttgtatg ctacatacta
    18361 attggtttta tttttctaat tcattaacat gagttaagac ttgttcgaga atactaaaaa
    18421 catgaaggtc atctaaactg taaagcatac tttttccttc tcttcttgat ttcactaagc
    18481 gtgcgtcttt caacgttttt aattgatgag aaatagcgga ctgttccatg tccagagaaa
    18541 tagcgatatt tccaacacta cactcttttt tctgtaaaag aaagagaacc gacaatcgtg
    18601 taggatcact aatcacctta aatagtttgc ttattgacag aatagatcct ttatcgattg
    18661 gagaagttgt ttttatatcc atagtgtttt tattcctttc atatgacctt ccgttcatat
    18721 ctatattacc atatgagcga aacaatgcta atcttttgtt tataaaagag aagtggcgtt
    18781 ctctttatct tgacaaaatg tatatagcga tataaaatga gtatacaaac atatgattaa
    18841 ttagtcatat gtaaggtgga ttcattacga tttacttttg gaataaacga aaggagtcag
    18901 aaatagcaga aatttatgtg attcaagaag taaatgatgg aaaacaaagg aattataaat
    18961 tatataggga ggcaatagaa gtggaaacta tccaagaaca acagtctcaa gaaaagcata
    19021 atcataatca caaccacgat cacgaccatg atcatgggaa aatgccagtc gttttgtact
    19081 ttgttggttt agcattagca gtgattgctc tatttttgaa tgaagattat cagttggtac
    19141 aaaatgtttt gttctcaatc gctacaatca gtgccgggta tcacgttatt cttcttgaag
    19201 gccttggaga aacaattgag aattcaaaag cacaaaaaaa gttcatacct aattctcata
    19261 ttttaatggg attagcggca attggagctt ctttgatggg gaatttttgg gaaggaacat
    19321 tattgattct tattttttct ggcgcacatt ttttggaaga ttatgcagaa ggaagaagca
    19381 aacgagaaat aacgaaatta ctcgaaatga atccaacgac tgcccggtta atcctgcctg
    19441 atggtagtac aaaaaatgtg gatgtcagtg aattaaaagt gggagatcaa cttcaagtgt
    19501 tgaatggcga tcaagtacct attgatggcg tcattttgtc tggatctaca tccattgatg
    19561 agtcgtctat tactggagaa agtatcccga aagagaaatc caaaggagat ggcgtcttcg
    19621 ggagtacaat caatggaaca ggtaccttca cgatgaaagt caccaaagaa aacaaagata
    19681 cagtattttc aaaaatttta caattagtga atcagaacca agataaccaa acaaaagctg
    19741 cgagtattat ccaaaaattt gaaccgaaat atgtcacagt tgttttaatt gccattcctc
    19801 tattcatgtt attggctcct tttctacttg attggacatg gtcacaaagt gtctatagag
    19861 gattggtcct tttagttgca gcttcaccgt gtgctttagc agcagctact gtctcggcaa
    19921 cgctatcgac gacttctaat ttagccaaaa aaggagtcct ttcaaaaggt agttcttacc
    19981 tgtctcaatt agccgatatt aaagcaattg catttgataa gacgggcacc ttgaccaaag
    20041 gaaaacctga agtaaccaat tattattttg ctgattccgt gaacgaagaa aacatgattg
    20101 atatcgtagt agctcttgaa aaagaatcca atcacccctt agcagatgct attctaagaa
    20161 agtttgagca aaaaaacaaa ctaactattg aagtagaaaa ccaaattggt aaaggattga
    20221 cgggagatta caaagggaga aattatcgta taggaaaacc gacttcgttt gatgatgttt
    20281 cagatgaata tagtcgttta aataatgaat gggcgtcaga aggtaagaca gtcgtatacg
    20341 tagcagtaga tgaagaggtt ataggcctta ttgcccttat ggacgttccg agtgaatatg
    20401 ctaaagaaac cattgaatat tttaggaaac aaggtataca caccacatta attactggtg
    20461 actcagaaat gacgggaaaa gcggttgcta aacaattagg aatagatgaa gtgattgcga
    20521 atgtcatgcc ggaagacaaa tccagaatta tagacgaaca aaaagaaaaa tacggcgtga
    20581 ccgccatggt tggagacggt gtaaatgatg ctcctgctct tgttaatgca gatgtgggaa
    20641 tcgctatggg agatgggact gatgtggcag tagaggtatc tgatttagtt ttaatgcaaa
    20701 acaatttatc taaattggta caatctcata atatttcttc gaaaatgaac cgtgttattt
    20761 ggcagaatat tattttttca atggccgttg ttgctttttt aattgtcgtt agtttcttag
    20821 gcttaacgga tattgcaatc agtgtcatta ttcatgaagg aagtactttg gttgttattt
    20881 tgaatggact tcgattgcta aaggcagtct aaagaatgat tgcacaactt caaattaatc
    20941 gagtaataga gataattaag gattacacta cattttttat ctctattact tgtttatatt
    21001 tatctagatg ttcaaagtca aaatgatata tcaaccatac tcttgtggcg cgggttgaat
    21061 ataagataca tgaaattgga taaaatcatg tatcaagaat cctatcctta tagggttctt
    21121 tttttttact ccccctaaac acacgatttc gtgttgtaat aactataatc atgtattcga
    21181 acagactaaa aaggagggat tgtcatgggc tataatgttc agccactacg gtcgcaacag
    21241 gagattaatg attttttatt ttgcctgcga cggaataaaa atgcggaacg tgacgttttc
    21301 ttgtttctaa ttggaattaa cagcggtctg cgcatgtccg acatcgttaa actcaagaaa
    21361 aaagacgtga ttacctcaaa aaacccgcgc atcgtggagc aaaagacagg gaaaacacgc
    21421 attttatact taagcagctt acaggacttg attcacggct ataccgcacc cttagatccg
    21481 gaggcttact tgtttcccag tactaagggc ggccatttag aagtcaatac ggtctaccag
    21541 atgtttcaaa aggtcgcgaa gctgttagga agggacgata tcggcaccca caccctacga
    21601 aaaacctttg gttaccacta ttacaagaaa accaaagacg tggccacctt aatggaaatc
    21661 tttggtcaca gcagcgagaa gattaccaaa cgctacatcg gaattaatga agatgaaatc
    21721 agtgaaacgt tattaaattt tcggttaggt ttttgattgt attatataat agataagcac
    21781 agtaagacgg ttataatcac ccacagtatt ggacaaatca accttttttt ggtatattat
    21841 atatacaaat gagagcaggc tcatagtcaa cttgatataa atccggaaca taagggtccg
    21901 gcgggtggcg agcaccaaaa ctaaactcga tacaaatccg gaacgtaagg gtccggcggg
    21961 tggcaagcac caaaactaaa cctgtacaaa aggctgctct tttgagcagc tttttttatt
    22021 gggaaaaagg agcattcttc gcacgcggca aaaaaagaat acaaacgcat gtctataaaa
    22081 gagacaaccc aaattaccag acagttaaac gcgatttata aggctgctca tctcttacaa
    22141 gaacattttg tagataaaaa agtatccttt gtcggagaag tctcaacggt agcgattatt
    22201 ttttcgacaa ctaattttat gcacctctgt ggcattgatt atcgcagagg cacacacttg
    22261 ttttttcaag atgccttaga caggaaaata aatttacagg atattcaaat caaaacggat
    22321 gggacgacct ttcagaaatt acaagtaata ggtagccttg atttattatt ggggaagcat
    22381 atttctattg ttggcagagg tgtttactct tctcttcgct atgatgcagc tattcgaaca
    22441 aggaaaaaaa tattggctct gtctttgaaa caaaacggat taatctatat ccctatctct
    22501 ttgttaaacc tatcatcaaa ggaaattgga cctggacaaa aagttactgg catttttagc
    22561 gaggatttaa catccgggga acttaaaatg attatggaag tcatcgatta atcattaata
    22621 gtgatatgcg acgtcatttt gaagtaatta ttctattttg ggttataaaa taaagcattt
    22681 taagacgatt tcaaatcatt catgtgggtt at
//
LOCUS       DANKEF010000041        22464 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000041 DANKEF010000000
VERSION     DANKEF010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22464
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.041"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            144..542
                     /locus_tag="PWZ48_001836"
     CDS             144..542
                     /locus_tag="PWZ48_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDL2261492.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
     gene            526..1220
                     /locus_tag="PWZ48_001837"
                     /pseudo
     CDS             526..1220
                     /locus_tag="PWZ48_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(1814..2701)
                     /locus_tag="PWZ48_001838"
     CDS             complement(1814..2701)
                     /locus_tag="PWZ48_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDL2261493.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            2886..3725
                     /locus_tag="PWZ48_001839"
     CDS             2886..3725
                     /locus_tag="PWZ48_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2261494.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            3921..4895
                     /gene="sgrA"
                     /locus_tag="PWZ48_001840"
     CDS             3921..4895
                     /gene="sgrA"
                     /locus_tag="PWZ48_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HDL2261495.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            5193..5549
                     /locus_tag="PWZ48_001841"
     CDS             5193..5549
                     /locus_tag="PWZ48_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261496.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            5552..6709
                     /locus_tag="PWZ48_001842"
     CDS             5552..6709
                     /locus_tag="PWZ48_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261497.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(6735..7175)
                     /locus_tag="PWZ48_001843"
     CDS             complement(6735..7175)
                     /locus_tag="PWZ48_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2261498.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            7335..7841
                     /locus_tag="PWZ48_001844"
     CDS             7335..7841
                     /locus_tag="PWZ48_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2261499.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            8009..9922
                     /locus_tag="PWZ48_001845"
     CDS             8009..9922
                     /locus_tag="PWZ48_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HDL2261500.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            9927..10928
                     /locus_tag="PWZ48_001846"
     CDS             9927..10928
                     /locus_tag="PWZ48_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2261501.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(11130..11336)
                     /locus_tag="PWZ48_001847"
     CDS             complement(11130..11336)
                     /locus_tag="PWZ48_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HDL2261502.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(11982..12830)
                     /locus_tag="PWZ48_001848"
     CDS             complement(11982..12830)
                     /locus_tag="PWZ48_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2261503.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            12829..13896
                     /gene="gnd"
                     /locus_tag="PWZ48_001849"
     CDS             12829..13896
                     /gene="gnd"
                     /locus_tag="PWZ48_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HDL2261504.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            13898..14899
                     /locus_tag="PWZ48_001850"
     CDS             13898..14899
                     /locus_tag="PWZ48_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HDL2261505.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            14896..15180
                     /locus_tag="PWZ48_001851"
     CDS             14896..15180
                     /locus_tag="PWZ48_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDL2261506.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            15213..16010
                     /locus_tag="PWZ48_001852"
     CDS             15213..16010
                     /locus_tag="PWZ48_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2261507.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            16024..16857
                     /locus_tag="PWZ48_001853"
     CDS             16024..16857
                     /locus_tag="PWZ48_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2261508.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            16875..17732
                     /locus_tag="PWZ48_001854"
     CDS             16875..17732
                     /locus_tag="PWZ48_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2261509.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            17754..18263
                     /locus_tag="PWZ48_001855"
     CDS             17754..18263
                     /locus_tag="PWZ48_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2261510.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            18300..18701
                     /locus_tag="PWZ48_001856"
     CDS             18300..18701
                     /locus_tag="PWZ48_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261511.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            18813..19166
                     /locus_tag="PWZ48_001857"
     CDS             18813..19166
                     /locus_tag="PWZ48_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261512.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            19200..19643
                     /locus_tag="PWZ48_001858"
     CDS             19200..19643
                     /locus_tag="PWZ48_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2261513.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            19660..20745
                     /gene="uxuA"
                     /locus_tag="PWZ48_001859"
     CDS             19660..20745
                     /gene="uxuA"
                     /locus_tag="PWZ48_001859"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2261514.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            20738..21451
                     /locus_tag="PWZ48_001860"
     CDS             20738..21451
                     /locus_tag="PWZ48_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2261515.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(21658..21807)
                     /locus_tag="PWZ48_001861"
     CDS             complement(21658..21807)
                     /locus_tag="PWZ48_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261516.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            22203..22403
                     /locus_tag="PWZ48_001862"
     CDS             22203..22403
                     /locus_tag="PWZ48_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDL2261517.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         7746 a         3451 c         4641 g         6626 t
ORIGIN      
        1 tccatacatc cattgtactc tgagaaatca cacaatatcg aaacaaattc cgttaggaat
       61 gtgttattca ataaatttta taatctagaa acattttgaa taaaaactat tagacaaatg
      121 atattttacg aggaggaaca gagatgacga tctttgaaac ggttgtaaca gatgttggta
      181 tagaagcaga agtattcagg aaagaaaaaa taatgatttt atttggagat gaagcaccgg
      241 aaacattaac agattattgt tataagattg aattgaatca ggtaagaaaa gaaataactt
      301 gtgatatgaa gctctgtttt gataaacaaa tgtatgtaat cacagcagta ggagatgttg
      361 tccaaactaa tttgaatgaa ttaggacata tcacgataaa atttgatgga tctcatgagc
      421 cggaacttcc aggaacaatg tatgtagaag ataaggaaat gccgcattta agaaagggga
      481 caatcattaa aatcgatcaa gaggatcaga atgggggaaa gctatatgga aatcatgctt
      541 gatacagcaa atacaaaaag tatttattct tgtcaacagt ttttgaattt aacaggcgta
      601 acaacaaatc ctacaattct gaaaaaagaa ggagcgtcaa acttcttctc gcatatgaaa
      661 gaaatcaaaa aaataatcgg acagattcct atccatattc agacaatagg aagaacggaa
      721 gaagaaatga tagaggatgc tcgagttatc atagaagaac tgggaaaaga tacgtttatc
      781 aaggtgccgg tgaatcaagc aggccttcaa gcgatcaaaa agttgaaaaa agaaggttat
      841 ttgattaccg gtacagctat ttacacagaa ttacaagggt atttagctat taataatgga
      901 gcagattata ttgcacctta ttataacagg atgcaaaatc aaggcattga tgctatgaaa
      961 gtgattgatt cgttgcggac tgagatcgaa cgtacgcaaa gaccaacaaa aatattagca
     1021 gctagtttca aaaattgtga acaagtcact acagccattc gatctggagc tcagcgataa
     1081 cgttaggtaa agagctacta gatcaagtat tagagatgcc ctcgattgat aaagcagtga
     1141 ggggcttcca agaagattgg gtgcaaactt tccaagcaga aacgattggt gaactaaaaa
     1201 aggtcgctga ttacttctga atcaatttct ccataaaata aacgatagcc ttctcgaaaa
     1261 ctcgaggagg ctatctgacg attcaatatt cattaattta aagtttgggt gactattact
     1321 aactattact tatttctcat ttaaacgatg tattccctaa cctctaatac atccttgtaa
     1381 ctctgaagtt acgccagttt tgaataaatt tcataaaaac aaatgaaaag tgtcgcataa
     1441 aaatatcttt cgtgtttctc tagtataaca gaataagaag aacaagtcca gaagggatat
     1501 ttttagtctt ttttaaaata acttttcagt cgagtttgag tttatgatag ttgtttttga
     1561 agcgatacgg caagtgaaaa aatagggaag catgaaagaa tctacttaat ggcgcattga
     1621 ttcatgtgat agaaataaaa aactttttta aattatatcc tcatgattgt aacaaatcca
     1681 gtgatttgtt ttggtacaaa taaaaaaagg ccccgatgaa gttgtggcaa aagtggtttg
     1741 cctcaaaaat aagaaggaat ttccaaaaat cacttctcaa attttgccac atccccaatt
     1801 gggtcaaatt tgtttatttg aatatatttt ttacataagg atctttaata gttacattgt
     1861 cttttttcat cactttacgg attgttttat ccatgaattc cgtatcaaga gtttttgctt
     1921 cggtagcaat tttttctagc tcagatttgt atttgtcttt attagaacct ttatcctgtt
     1981 tttttaccat ttttactaga taatagcttt gctgataagt cgttggatct gttacaggaa
     2041 tgatatcaga aacttccccg ttttttagtt taaatgctgc tttttttact tcagtaggaa
     2101 tctctggatt ggttgaatca aaattcattt ttccatcagt ttcctttaat gttttatttt
     2161 ttccataagc ttgaacaagc ttggagaaat tttctccttc atttgcttct ttttttgctt
     2221 tatctgcttc ttctttagtc gaaaatagaa tgatctgtgt actaacttct ggatagaatt
     2281 ctttccaagc agtgtctaga tctttatctg ttaattttac attggctttc aaaccgtatt
     2341 ggaaagccag catctgttta aagagtttct tatatgtctc ttctgtttgg ccaactgctt
     2401 ttaattgttc cttgtagttt ggaccgagtt ttttgatttg ttcattatat tcttttgtta
     2461 cttcctttga agaaatctct tttccgtata catcatcaaa tgctttgaag acaatcattt
     2521 tttttaatag tgtttgatta tttgatttaa cttttgggtt atcataaaaa tcctgaactg
     2581 taagtgttcc acctttaaac gttgcaatcg tttcattgga cttgctacaa cctgagaata
     2641 gtaaaattgc acatgctgaa aggcacataa ttagtagttg ttttttcaaa aaatttccca
     2701 tcctttcctt attaaaaaca gtctaacaga agaattaggg agacagtctt tcattttcgt
     2761 aaggttgctg tagatattct tagttcgtaa aaatctattc tctctatgat ggagaacatt
     2821 tacatagttt tcacgagaca agaaagaaaa gggttgttaa acttaatata aaggagggat
     2881 gaaagatgat caggattatt cggttgattg gatctgtttt aatagtcttt gttggttttg
     2941 ttttaggaat tcttgtcaat aatgcacttg cggatatgtt tattggaaat agtgaatgga
     3001 ttggagcaat tgccggtacg gtcggacatt tagctgtttt tttactagct ttatttcttg
     3061 ctagtaaggt cgaaggaaaa agaatagaag attatggtgt ttctgttcga ggaaaagact
     3121 gggggtattt agcgggaggt ctcgttgttg gaattggagt gtttctgctt attactagtc
     3181 cattatattt gacgggggcg tatcggttag attcaggaaa cgcaaacatt gtgccgctga
     3241 taacgagttt tattttattt attgcagtgg gggcatcaga agagctttta ttccgtggct
     3301 tttttcagca tcagatgctt agatttggtc cgttaatagc aatgattgga tctgctgcat
     3361 tatttgctct tttgcacgga ttgaatccta acatgacatt tctggcagtt ttcaatatct
     3421 ttttagcagg ttgctttttc agcgcactta tctacagtac aggaagttta tttacggcta
     3481 ttggtgctca tattacttgg aattggacac aaggagctat tttgggtata ccagtcagcg
     3541 gaacgcaaga acaaggtttt ttctccactc tgatccaatc gcaaaataca ataattactg
     3601 ggggaaactt tggggctgaa gcatctatat caactacatt attcttgtta gtcttgattg
     3661 ttggtttgtt actctatgca tataaaacaa agcggatgaa gaaatacact gctgtttatc
     3721 aataaaagat gaagaaagca aggctgatat cggtcttgct tttttaagtc tgtcattgat
     3781 taaacgaaaa agtaaacaaa ttcaagcgaa tacacgacat cttttgttag attttcacaa
     3841 ctgactgtcg tgttctcatc attttcattt tatataattc ctttatcaaa tagaaggtaa
     3901 ttatatggag agtgatagaa atgaagaaaa cagcgaccgt attatttgtt attcttgggc
     3961 tgagtataat tgcagcaatg attggaatca gtacttctgc aaatgaacgg gcaaatgaga
     4021 atctttcatt tactgtgaaa actgatcgga ttgtttatga catgacacaa caagtcatta
     4081 ctattccagt caaaccaaac aaaagcgtga atgctagtga tgtacatgct gttctaacat
     4141 atggttggga tggtaatgga agttcagaaa aagttattgg ggaagtttat ttaaaagacg
     4201 tacaatggac cgctggtatt gagtatacaa taatgatttc tgcagaacta tcgattgatg
     4261 agatcaagag taaagataaa gttgatttga ttgtttttta tgatggacag atgacgatca
     4321 cggagaattt gaaaccaagc agttggacag ttgttggacc gccaagcagt acggatagca
     4381 caagtagtga aagcagtaca gaaaacacga gcggtgaaag cagtacagag agtacgagca
     4441 gtgaaattag cacagaaaac acgagcagcg aaagcagtac ggagagtacg agtagtgaaa
     4501 gtagcacagg aagcacgagc agcgaaagca gtacggagag tacgagtagt gaaagtagca
     4561 caggaagcat gagtagtgag agtagcacag gaagcacgag cagcgaaagc agcacaggaa
     4621 aagtaaataa tgaacttgat ttggtaccta tcataccaac taaggaacaa aagagaaatg
     4681 cagggagcaa cctaaaaagt gtgctaaaac agcctgattt tgtttctcgt ggaattgatc
     4741 atcaagaaaa agcgaaagaa acagataata gaaaaagcga tttaccagaa actggtagta
     4801 gaaccttgaa caagctaatt acatggatgg gtgtgttact cattttgatt gtaggagcaa
     4861 gctattttcg ttctctcttc catcgagtga aataaagaat aaaagcaaca gatctaaaat
     4921 tgaataaaac gatagatttt tgagtgaata tttcattcga gagtgctgta ttgcctggcg
     4981 aagttgtagc tttgccagat ctatttttgt ttactaacat atttgagaaa caggaaataa
     5041 caggaacaca aatggaaaat aagagcaaga ataattgaca atgcacactg cgcatggtag
     5101 tatctctgaa aaatcagtaa cgaatctatt tgtacgttat tcatgtagct gtcggaataa
     5161 gtctaaataa tagataaaaa ggagtatgta ctttgtttga agaatttatt gatatcaatg
     5221 aaaggcaagt atatcaattt ttgaactatt gttatgaaag agacgagaaa ttatatgtgg
     5281 tgaaggatat cgctttagat ctaaactaca ccctggctaa gatgaattcg gtgattcaac
     5341 aagcagaatc gttttgcgaa aggtatccag aatataaatt atcttttctt tcagaaaaca
     5401 agatgataaa agtcgagttt tcttctcagt ttttactgag taaagtttat tctatacttt
     5461 tagagggaac gattggttat attttactgg attcacttta caaaggtact tatcaatcct
     5521 tagaaaatct tagtcaaaaa attatataag tttgcgtacg attcaaagaa aattaagaga
     5581 acttaatact attttgaaaa actatcattt atcttgcagc ttgaaaaaag gtaatccgat
     5641 agaaggagaa gaataccgta tccgatactt ttttcacttg atgtattggc agtcattcga
     5701 cgaaaatcat tctgatatat tatcaagtat aaaaaataaa cgtcaactat tagaaaaatt
     5761 tgcgaaacat gcgccatata cgcgtagtat agatagaaag aaatggatca gcctactaag
     5821 aatttcgttg caacgaatga aacaaggcca ctcgattacg tatgtcccag aagagatgaa
     5881 caaatttgtg catccattga taacaaaaga agagtttacg cattacatac tagagccttt
     5941 ttttgaagca aacttctggt cgatgaatca catcactcca acagagcaag cttaccttta
     6001 ttttatgttt ggtgtaatga acacctattt ggaagaagat ttgttggata ctccagataa
     6061 agtacaagaa agttggggct ggattgaatc gtctattgta gaaaattttg aaaaaaactt
     6121 caagatttgt tttacaactt cagaaaaaaa ttatctatat gtcaatttgg caatgatcca
     6181 tttgtattca agggtttttg ggacaaaaaa gaaggtggat gcttttggaa aatcagcaga
     6241 agacagagat tttcagcata tatttccagt gatgtatcca atcatgcaac agttttttag
     6301 tggggtagct cgcagaattc cagagcttaa tacttattat gaaaagaaca gacggttgat
     6361 ttttcaatac tgtatgttag tcagggatat ttttataaaa cacgaacaac ctgtcatatt
     6421 ctatattcaa tcaaagtatg gtaaaacaca ggaattatgg gcaaagaaaa ggatattatc
     6481 tatgactgaa cgcccaataa aatatagtgc atccgctgat cagttacctg atattgtcat
     6541 tagcgattat cctatcgaca aaaaggcata tgatcaaacg aatacacaga tattttactg
     6601 gaatggacaa cctacaaaaa atgattgggc gcgtctgaaa gctgaaatca taaaaatcag
     6661 gaatgagaaa aatagatcca tattggaaaa attggatttc gttaattaag aaaagcagat
     6721 tgtttatgcc aaaattaaga cattgcaaaa agattatttt ctttaaaatc gacaagatga
     6781 ccagaaagca agagttcgtt gagctcttgc tttcttttag ttttagaaag gaacatcctg
     6841 attttatctt cgctaaatcc agtcaaaagt tttgcagatt ggtcgtagat aatcggtttt
     6901 ttcaataaag aaggggatgt acgaatctca atatatagtt cactcaaact gagttcatct
     6961 attctgtgaa caagattttt ataactaata gaatgagtag agagcatttc ggtcacgccg
     7021 ttgtttgtca attttagtaa gtggataaat tcatttttgg ttagtggttg acgaattaag
     7081 ttctgttcga taaacggtat atcatggacg ataagccaac gttttgcgcg tcttgaagac
     7141 gagcagccag aacctgtata caatttaatc atcatacatt cctccaatag ctaagtattt
     7201 atgaaataat aacatgaaga aatagtcgaa aagtgccatt tagaggtgta aaataagaaa
     7261 atgttgtcac gtataatgga actagctaga taagaaatac tattaaatca agaaaaaaga
     7321 aagaaggcac aagtatgtca aacacagccg acgcaatagt agtgatcgat ttacaaaaag
     7381 gggtatgttt tgattcttcg ccgatacacg aattagaaac agtgacagca ttagtgaata
     7441 aaagaatcaa aacatacgaa acgattggga aaccagttat tttcgttcaa cacgaagatg
     7501 ctcaattagt gccacaaaca aaaccatggg agctattgtc ggaattatct attcctgagc
     7561 acagttattt tgtgagaaaa acccatgcga attcatttta taagactaat ttgagtgttt
     7621 tattaactga attggactgt cattcgatcg aattatgtgg ggcacaagca gagtattgta
     7681 tagatacgac tgtgaagttt gctcatggat taggatacca gcttatgatg tgtagaggag
     7741 caacgacaac ttatgataat ccttttatga aagcaaagga aacaatcgct ttttacgaga
     7801 aaatttggga taaacgattt ctgacatttt tggagcaata agttgtttct tcgtttaaaa
     7861 aaagcagaat ggatactggt ttatccattc tgctttatta cgtaaaatga gaaaataatc
     7921 ctgttctatt tttatgggat aaaccattta ttttttgtat aaaaaatgtt tgcgtttaca
     7981 tataattctc ctagaaacat aaaaaggagt gaatagcttg agaacaaaac aagtattaaa
     8041 gggagcgttg gtcatcttat ttgctgtaag tacaagcgtc ccgcaatgga cagtatatgg
     8101 agagagcatt tcttccgaat cggaatctcg ggaggtagaa accacagaaa ctactgatgc
     8161 tggaaatgaa acgaaatcga gtacttcaca aacagaaaca aatactgaaa caacggttga
     8221 gacatctgtt actagcacag atcaagaaac gaccgctacc acagataacc aacaaacaga
     8281 agaagcaaca gctacaaaaa tgtcaaacga cgaatcctct tctgctaaag taagtgacaa
     8341 agagaatggg acagaaaatg atgatatcgg taaaggtgaa aatttcaatg gtaaagaata
     8401 tttgaattac ttcggtaaac agatgacaga aatacttaat ggaccaacag aagacatcaa
     8461 gtcaaaatta atagagttat tagtcactga gcttgctgtt aatgattatg gtgtagataa
     8521 agagattatc aaacatctga ttgctactta tctattgtac gatatcgctt ttgcaaaaaa
     8581 cagtgtgttg atgtcgatgc ttggaggtac agatgatggg acaacgccta cagaaaacac
     8641 aggcttctat ccaaatacaa aacaacttca tcaaactgta ctaaacaaac aagatggcaa
     8701 aatgatggat gtatatgcat attatgtcga tcaaggttca gataaaacag tcatgatcca
     8761 tggcggattt cgagggaatt ggaataacgg gatcgttaca gaagaataca atgattttta
     8821 taaggccggg tataatttat tatttgttga ttccagagcg actggtaata gcggaggcga
     8881 ttatgtgacc tatggacaat atgagtcaga tgacgtcctt tactggataa atcaagaagt
     8941 tcgagagcgg ccatctcaaa agattttact ttatggtggt tcgatgggag cagctacgat
     9001 gatgtctgta ttagcgaaag atattcctgt caatgtcaaa ggaattattg aaaattgcgg
     9061 atttgcatca attgatgaac aactacgttt tacgtacagc cagacagtag cccccgcact
     9121 accggatgcc ataaaaaatc agttagacat cattggggat caagagcatg aagatttatt
     9181 tatgggcctt ttgaaacaat actattttga tcaggagatg catttggata caaaagctgc
     9241 tctacctacg attggaatgt cagattcatt gcctaaattg attatccatg gaacagcaga
     9301 tgatgtagta cctgtgtcta acgcgcaaaa attatatgag ttatcaggag ggtataaaga
     9361 tcttttatta gtagaaggag cagggcatgg aaaggcacag gaagttgacc atgctgccta
     9421 cacgaaacat gtaacggact ttttgaaagt agtgttccat gatcagataa acgtaaagta
     9481 tgtggatgaa aacaataaaa gtctactcaa agaccaggac gaaatccagc tttacggagc
     9541 ttatggagaa aattatatga cagagcaaaa gacattcgaa ggatacgaat tagcaaatgt
     9601 tgaaggaccg actgaaggga tttttaatga gacgatacca actattattt tctattacaa
     9661 aaaaatccca gtagctccac caaaaaagca agatccgaca gagaatgcag acaataaggg
     9721 aaagaatgaa cctcgagagg atcaatcaag tgatgatacg caaccgattc agatgacagt
     9781 gaaagtacaa aaaaagacag atgcaaaaaa aagaatgttg ccgaaaactg gagaaaagaa
     9841 acattcgctt ctcatttatt caggtggatt tttatctgtt agcacgatta tcacatggat
     9901 ttggcgaaga aagagagggt aaatgaatga aatggttcaa gcgaatagtg attgcagtac
     9961 cagttatcat cgtagttatt ttagtcactg tatttttgat taatcaaatc acaccaaagc
    10021 cgattagctt gcttgttaga aaacaatttg acacaagtga aaaagaacaa gtatatgccc
    10081 gaccagatga tttccagcaa aaaatcgaac aaatcgatat aaaaaaagaa atatcctatc
    10141 cttcaaagta caaaaataat aatatggata tctacacacc gaaagtggaa aagaaaaaat
    10201 taccgattct tttctggatg catggaggcg catatgtagg aggggataaa aatgattgtc
    10261 gtgattattt ggaatatcta tgttcagaca cgcagcaaat catcgtaaat atagattatg
    10321 aacgatcacc ggaagcaaaa catcctacac cagttatcca attgaacgaa gccattcaag
    10381 cagctaaaaa agagataaaa gatcaagcag attggtcgaa tatttcgatt ggtggaaatt
    10441 cggcaggtgc acaaattagt ggagaatatt tgttagcact tcaaaatgaa gaaattaaaa
    10501 aagctgatca tcttgatccg actttggaga acaagcagat tacgaagttt atttctttgt
    10561 ctggtctgtt ggatccttct gcacttactc aagtaagtga taaaatctct agctttcttt
    10621 atgaaaaatg tggatgggct tactttgata ataaggactt tgaacaatca gagaaggtca
    10681 aaagtttagc cttggtcaga catgcggatc gttggacgca aaacacattc ttaacggatg
    10741 gaaatacaaa tacgtttacg aaacaaatgg aagaagtaac ttctgtattt gagaaagcgg
    10801 gggtgaaagt cacaaaagta gattatgcgg agaaaaatgc tgttttgaac catgaatatc
    10861 agtttgattt cagcaataaa caagcgcaag aaacatatca aaagttagta atgtttttca
    10921 aggagtaaag tcttatgatg caaaagaggt tgtaatctgt cacttagctc caagtcacaa
    10981 ggagcaattt caataaacaa gcttttcatg gtttatgaat attgtaactt gtgactgagg
    11041 agttagagat ggcaaggggc accttttatt cgaaatttgg gaatatcaca cgattattgt
    11101 cacgatctct tttacgtaaa ttttataagt cagatttgtt gttttgatcg tttaaaaaaa
    11161 gcaacaaatc cgaagcctgc ggaggcaaga agggacaaaa agccgagtgg taagcaaaag
    11221 aatgctggtt ccactaatat tccgttgtta ttgatataag gctgggaaca aagatgaata
    11281 gcaaataaaa tcaatgcact agcgaaaaaa agcaaagaaa tatggatagc tttcataagc
    11341 agcacctcct tttttataat tgtatgataa atagattaag aatctataaa tgtgacaagt
    11401 ttgttaggat gtgaataaaa tgatcatcta gcgaacttga aacaaagatg gctaatgata
    11461 agcaattaat gattcagcaa tcagataggt acaaagttta ccatcatacg tctagaccaa
    11521 taatagaaaa tatgggaata taatagaaaa gatttaggat acccccaaaa gaatttcccc
    11581 aaaaattcga tgtatcctaa acttttttat tttacataac aatcatatca actgtgaaat
    11641 aaaattacaa atgataacct aaaatgaggc caaaagccag tgttatcaag gaaatactta
    11701 attcaccaaa atttttttta attttttaga aaataagaaa caaaaataat aaaaaaaaga
    11761 aaagataaat tttttactac tgtatactaa ctttgttatc gcatctaaca agtaactcga
    11821 ataataccaa tacaaccttc tccaaagatg cgatgacata cgttctaacc caccgactat
    11881 tgatgttctg taccaccctt gcagaaccaa aaaagccact accaaatatt tggtagtggc
    11941 tttttttgtt ttatggattt ttcgcttttt ctgttatggt gctattcttt gatttcagaa
    12001 atagtatgtc gaactttagc gatagaacga cgtgattctt tttcgttgta aaacatcaaa
    12061 atcacatata gagagtccaa aatgcttagt tgagaaatac gggaagctaa agcttcagat
    12121 cggaattctg tttcttcaga aatagaaata aaaacgacat cgccaagttt agcaagggaa
    12181 gaattagctt ggctagtaat aacgattgtt tttgctccag tttttcttac ggtttcagca
    12241 atatggattg tttcttttga ttgccctgtg tgagaaatca aaacagcaca gtctttttcc
    12301 gtcaataaag aagcttccat caattgaata tggtaatccg tgctgtgaga agttgcgata
    12361 ggggaacgca aaaatttatg ataagcgtca gttgctacaa tctctgaacc gccaacaccg
    12421 aaaaaataga cacgtttaga ttcattgata atagtagccg ctttttttag atccttttgt
    12481 tgtaacaact tttttgtgtc atttaaagtt gaaatactag actcgaacac cttttgcgcc
    12541 atagtgagct cagtatctgt tttctgaata ttttcgtgga tagaaatagc agatagatca
    12601 ttttcttgga tcaacatggc cattttaaaa tctttaaatc cattataacc aagttttttt
    12661 gtaaattgaa aaaaagtaga gtcagcaatg cccagttcac ttgctaaatt gctaatagga
    12721 ctatgactta ctttgcttgg attttctaaa atataatcgg cgatagcctg ttcttttggg
    12781 ctgaagtcgt tgtacattgt tttgatttgt agtcttatgg agacgctcat gagaattcct
    12841 cattttcata ataagattat cattttttcc gttctcatta atgttaaaga tacaaggaga
    12901 gtaggaaaaa atcaaacaaa aaatattttt gtaaaatcta tttacaaaaa aatttttttg
    12961 tataatgagg gtataagaaa tggaggcgct atcatggaaa ttggtgtaat tggtttggga
    13021 aaaatgggat tgaatttagc tttaaactta aaagagcacc atcacaaagt acaaggactc
    13081 gacatttcaa gtacagctgt tagtcaggca agagaaaatg acattcctgc ttatcaagag
    13141 atctctgaat ttctagctac ttttgagcag aaaaaaatca tctggttaat gcttcctgct
    13201 ggagagataa ccgaaaacat gttaagaaaa ctttttccaa aactcggacc aggagacatt
    13261 attattgaag gcggaaactc ttattataaa gattcaatcc gtcgagcaga agagtttcag
    13321 aaaaatgaaa tcggttactt ggattgtggg acttctggag gaatcgaagg agcacgacat
    13381 caagcctgct taatgattgg aggagataaa gaaacttttc tatccgtgga acaattattc
    13441 aaagatgtag caattgaaag aggatatctg tatgctggtg catccggaag cggacacttc
    13501 ttgaaaatga tccataatgg aatcgaatat ggaatgatgc aagcaatcgg tgaaggattc
    13561 caacttgtac aaaaaagtga gtatgctttt gacttggaac aagtcgcacg tgtttggaat
    13621 catggttcgg tgattcgcgg ctggctgatg gaaatcatgg aagatcagtt aaacgaacat
    13681 cccaatttaa gtaattatcg gggaatcgtt tctgcatcag gtgaagcaaa atggacggtt
    13741 gaaacagcgc tagaaatggc tgttccagtt ccagtaattg caactagttt gttcatgagg
    13801 aatctttcac aggaagaaga tagtttttca gcaaaagtag tttcagcact aagaaatggg
    13861 tttggcggac atgaaatagt tgaaaagggt gagtaaaatg ccagattatc atatagcaat
    13921 cgttaattcc agcagtttcg gaagagtctt tccacaccac ttggaaagat taaagtcagt
    13981 tggagacgtt catttcttta ctgttgatca agagattcca ggaaaagaac tagcggagtt
    14041 acttaaagga ttcaatatca ttattgcaag tgttacgcca ttttttcgac gagatttttt
    14101 tgaaaacaaa gatgaactgc tattgatttc ccgacatggt attggttaca acaatattga
    14161 tttaaaagca gcaaaagaac accagacaat cgtatctatc atacctgctt taattgaacg
    14221 aaatgctgta gcagaaaata atataactaa tttgttgtcc atgcttcgac gcacgacaca
    14281 agcacaagca agagtcaagg acgatcgatg ggaagatcgg gcggagttcg tcggtaggac
    14341 gctgtttaac aaaacagttg gtgtgattgg tgcaggaaat accggaagcg gagtaatcga
    14401 aattttgcgt aacggttttc gctgtgatgt tctagcttat gatccttaca aatcagattt
    14461 atacatcgaa agctttggtg caaaaaaagt agaacttcaa gaattactgg aacaatcaga
    14521 tatcatttgt ttatgtgcga atttgacaga agagaactat cacatgattt ctgataaaga
    14581 agtcgatcag atgaaagaaa atgtttatct atctaatagt gcaagaggcg ctctagttga
    14641 tgaagaagcg atgattaatg gtctgaaaag cggaaagatc gcaggatatg ccacagatgt
    14701 tttagaagaa gaaccgggac gtcaagatca tccgtattta gcgtttgaac aagtagtgat
    14761 tacgccacat atatcggcct acacaaggga atgtttagaa gcaatgggtg aaaaatgtgt
    14821 cagagatgtc gaacagatca gtcagcacct tcttcctgaa cgttctgtcc agacaaagag
    14881 caactatata aaaggatgat gaagaaaatg gtgaacggtt taaaagtgaa aacaggccct
    14941 caattttatt tatatgaaga aggtggtatt tcaaaagttt ctgatctctt gaaatcatac
    15001 ggcgcgaaaa gagtactggt cacccacgga acggtatcat gggaaaaggc gctacctaaa
    15061 ttagtatttt tgaatgacga aacgattcaa tttttttatc atcgttattc tggagaatgt
    15121 agttatgctg aagccagacg aatagctaca atcatcaaaa aaatgaaatc gatttcgtag
    15181 ttggcgttgg cggaggaaaa ctgaccgatc tagtgggtta tagtgccaat cttgcaagaa
    15241 tcgatttcgg agtcatccct actttagcta gtaattgtgc tccatggaca cctcttgctg
    15301 tcatgtataa ggagaatgga caagcagaag ggaaaacaga acacttttac agacaagctg
    15361 ctttttttat aacagatccc caattagtga ttgattcccc tgttccatat ttcattgccg
    15421 ggttagcaga tacactagca aaatggtatg aatctgagct catcttagaa caagattttt
    15481 tgcaagatga atcatttttg aagctagcac aagatactgc gaagatctgc aaagaagaga
    15541 ttttgaatca ttctgctaaa gcaatccaag atatggcaca aagaaaactg acatcggagt
    15601 tcaaacagct ttcagagatt atctttgctg ttgctggttt agttggagga ttaggagata
    15661 aatacgcaag aaatgcggca gcacatgcta tgcatgacgc tatcagtaaa tatattccgg
    15721 aaagccatcg ttttctacat ggagaaaaag tagcgtatgg gattttctat caactggcac
    15781 tagaaggtcg ctgggaaaaa atagacgagt tatatccttt gtatcgggaa ttagaactgc
    15841 cggtttctct agaacaaatg ggattatttc cgaaagataa acagataata gataaaatgg
    15901 ctgaatttat tgattcgaaa gagaaagtgc atttgattcc tatagaggta acacagaaat
    15961 cactgataca gtcaatgatg gaactggaag gttatatcag aaagatctga aggaggagga
    16021 cacatggaaa cattaagtgt agtacaaagt ttgttgattg ctttatgggt agccgcggtt
    16081 atgtcccgct ggttaggtgg aggagccacg ttgaccttac gtttttctcc tttgatgact
    16141 ggattagtcg tcgggttgat catgggggaa gttgcacaag cgatgattgt tacagccgct
    16201 ttgcaaatga tttatatggg ggttttttct ccaggaggat caatgccagc tgaaccatct
    16261 attgcagcag ctatcgcagt accagtcgca ttattagggg atctgtcgcc agaagcagcg
    16321 attgccgttg cggtgccagt cggattgctc ggaagttact tgtatcagtt tcgttttttc
    16381 atcaatactt ttttaggtaa atatacagac aaagctgtag aagatttgaa tacaaaagct
    16441 atggcccgat caattattct ttatccaacg gttgcctcat ttttattatt cgttccgctt
    16501 gttttcattg ctttatattt aggggcgcca ttgatcgctg atgtaatcaa agctttagag
    16561 ggaacagtcg ttatccacat tcttgaagta gtaggtggag ggttagcggc aatcggtatc
    16621 gcgacaactg tatacgttat cggtcgaaaa gattatctgg tctttttctt ccttgcttat
    16681 tttatgagtg ttgttttgaa atctttagat attacgatgg ttacttatgc catttttggg
    16741 attattatcg ctttgatctt tgtccagtcg caaaaaggga aacaagtaca gcctgccaca
    16801 agcaataaca attcagcatc ttttgatgac gaagatgact atgatgacgg attttaattt
    16861 aggaggaatg ggaaatggct gaaataatgg ataatcaaac aggtgctcct gaagaaatca
    16921 cgaaaaaaga tgtaacaaaa gcctatctga gatggcattt tgcaaacgaa attcctcact
    16981 cttttgaacg ttacttggcg ccatcactac tatacggaat gatgccaatc ttgaaaaaac
    17041 tttataaaga tgaagactct ttaaaagcag catataaacg gcagttactg ttcttcaaca
    17101 ctcaattaag ctggggtggc ggtgtgatta ctggcctaat gtcttctatg gaacagcaaa
    17161 gagcagaaga agagtatgaa gaaaaagaga tcttgatgca ggatgacttg atgtataaca
    17221 cgaaagcggg attgatgggg gcactagcag gaatcggaga tgcaatcgat tcaggaaccg
    17281 tccaatatat ttttatcgca atcgcggtac cttgggcaca acagggaagt cctttaggcg
    17341 cactttttcc ttttatctgc tttgggttgt atcaggtttt actaggggta tttttcgcaa
    17401 gacaagcttt cactatgggc cgtaatgcta caggcctaat gcaaaacact ggtgttcaga
    17461 cagcgattga aatgctatca gttttagggt tgtttatgat gggggtgctt gcgggaaact
    17521 atgtaaaagt agaatcttca ttgaaatttg cgatatccgg acgtgagttt gttatccagg
    17581 aaattcttga tcaaatcgtg ccagggatat tacctttagc tgtcgtaatg ggtgtgtact
    17641 tgttctatac gaaaaaagga ttgaaagtca cacaagcatt attatggctg actggaatat
    17701 taattgtgct agcaggaatc ggtattttat aaaaagaagg gaacgtgaat aagatgggtg
    17761 tagtcaattt agcgcgtgtg gatgagcgtt tgatccatgg gcaagtaatg gtaacgttat
    17821 ctcaaaaaag tggagtgaat tccatttttg ttgtagacga agttgtagca aaagataaat
    17881 ttatgagaga tctttacaaa aatgcaggaa gccgtacagg acaaaaaaca attgtcatta
    17941 caccagagaa agctaaattc tattgggatg aatatcaatt taaagaatac aattgtattt
    18001 tgatcgcaaa aacagtccaa acgatttacg acttagtaaa acacggaatc cccatggaag
    18061 aattgaatat tggagggatt gctcaaaaaa atcctgatac agatctttta gtgacgaaat
    18121 ctgtttattt aaataaatca gatgctgaaa aattgaaaga aatgaatgaa caatatggcg
    18181 taaagaatat ttattttcaa gccactccat ctgcacctaa aacaggatta aaagatgtat
    18241 tgaacaagtt tgatttgtca taaacaaatg cttagaaaaa agataaagag ggcgaaaaaa
    18301 tgaagaagaa cgagctgttt cgtgattggg aatttcgtta tcgctatatt taccgtaagc
    18361 gccgtacaaa aaagagtaaa cagcgttttc tttcagcatt agtatcagat atttacagta
    18421 tgagaacaga tgtgacagtc attgcttacg atacgctagc ctaccgatca aaaaatattt
    18481 atgttggaga tatcgaaaaa gcagagaaag ttatctgtac ttattacgat acacccgttc
    18541 atgcattagg aagttatttc atgtttgatt ggaaagacca aaggaaaaaa acaatctact
    18601 ctatattatt gtccttcatt ttgttgttca gtttaggttg gtggggaatg atgatatata
    18661 acaaaaatcc tcatcacgta ttcgatctgc tatccgtata aacaggtatc actgtactag
    18721 catttgggag ctactttttc ttattgggaa aagcagctcg cggatggcct agcagacaaa
    18781 cttttattcg aaatacttca tccatattaa cgatgctaga aatgatccgt acgattgatg
    18841 atccgagtgt agcgtatgcg tttgttgatg aaggctgtta tggagaaaag ggcttagaca
    18901 gcgtgagatc gggtatgaaa aaagaggcta ttctttttta tctagactct gttggggcag
    18961 atacaccgct acagttttcg ggaaattatt tttcaaataa agaacagtgg ctgaaacaag
    19021 ttgataagtt gaaagaaaaa aacgtcaatt atatttttag tgcaagaaaa aagcaagcgc
    19081 agttcttcta tttgaccaaa acagatttac gagggaaaac atttaattgg caaaatgcca
    19141 atcagattat tgctcttttt cgataagtaa aaagtgcaaa acaaggagtg agaagactaa
    19201 tgttggaaat catgatcgtt acacatggta agttgagcga aggactgaaa gatgcggcgg
    19261 aagtaattgt cgggacatcg agccatattg aaacaatgtg cttagagtct ggggaggaca
    19321 tccaaacatt cggagagaag gtcaaagaaa aattaactat gatcaacaca gcagaaccca
    19381 tgctagtatt tgttgatcta attagcgcaa gtccgtacaa tcaagcgctc ttggcagtca
    19441 atgaactgac tgaagaaaag aaagcagcca cttatgtcat agggggcgtg aatctgccaa
    19501 tgttgttaga agcaatcaat catcagatgc ttcacacacc gatagaagaa gcggtggaac
    19561 aaattgtcaa acaaggaaaa gataatttaa ctgtttggca tgcttcagcg gaagaaaaaa
    19621 aattagatga ggacgatttt tagaaggaag gctgaaaaga tgaagtgggg atttagatgg
    19681 tatggtgaaa aaggggattc gatcccatta aaatatgttc gccaaatacc gggaataaat
    19741 gggataattg gaactttgct gactaaaatg cctggagatg tttgggagat ttcagaaatt
    19801 gaggagttga aagcatctgt tgagagccaa ggactggaac tgcttggaat tgaaagtgta
    19861 gcgatccacg atgcaatcaa ggcagggaca gatgaacgag attactatat tgataactat
    19921 atacgaacaa tcaaaaactt gtctcgatgt ggggttcgat tgatttgtta tagcttcaaa
    19981 ccaatttttg gatgggcgaa aactgatttg tttaatgaaa acaacgatgg aagctattct
    20041 cttttatacg atcagaaaat cgtcgatcag atggagccag gagaaatgta tcaattgata
    20101 cacagccagt caaagggatt caaattgccg ggttgggaag aagaacggtt gaaaaaattc
    20161 gctcagctgg aaaaactgta tgaaggaatg accgaagaag aattatttga aaatctgact
    20221 tattttttag aaaaagtgat tccagtgtgc gaaaaagcag atgtaaaaat ggctatccat
    20281 ccagatgatc cgccatggga actttttggt tttcctagag tgacgaaaaa tcttggcgac
    20341 ttgaagaaaa ttctaaatgc agtagattct ccatataatg gactaacgct gtgcacagga
    20401 tctttagggg caaatccgga aaacgattta gttgaaatca tccatgaggt aggttcgcgc
    20461 atccattttg tccacttcag aaacgtggag tttttaggag aaagaaagtt caaagaaacg
    20521 gcacatccct ctacagaagg ctcccttgat atgtatcata ttatgaaagc gttggtgcaa
    20581 gaagggttcg acggtcctat acgccctgat catggtagaa ccgtgtgggg tgaagtggct
    20641 atgcctggat atggcctgta tgatcgtgca atgggactca cttacatgca aggtctatat
    20701 gaggcgataa gtaaggaaaa accactggga gagcattatg aataaaatca agctcttcat
    20761 ctttgatatg gatggccttt tattagaaac tggacgttta gcttatcgtg cttatgtaaa
    20821 atcagcacaa aagtatgatt atgaaatgcg taaggaagtc tattatttac taactggcca
    20881 aacagaaatg gcgataagag aacaaatggg cattttgtac ggagaagatg ttccatatat
    20941 caaatggcga gaagcaatca atcaatataa agaaaaaatc gtgaaagaag ataagagagt
    21001 atatacgaaa aaaggagcag aagaaatttt atcttttgca aaagaaagag gaatccatac
    21061 aatcgtggct tcgtctaata cacgggtaaa agtagaaatg tatttaaaaa tggaaaatct
    21121 atatgccttg tttgatcaaa tcatttcagg ggatgatgta aaaaaaggaa agccggaacc
    21181 agagatcttt ttgaaagctt gttcaaaaat gaatattcct ccaagtgaag cacttgtatt
    21241 tgaagactcg attgcaggga tcgaagcagc aaggcgagcg ggcattttgt cttttctcat
    21301 caaagatcat ttgatagggt tgcccgatca taatgggaag cataagttga aagtagatgt
    21361 aagtttatat gaatccaata aagcagacta tgaattttat gacctgcatc aagcaaaacg
    21421 atttttagaa agtaaagggt tgtatctcta ataaagagga ctattttcta gtcctcttta
    21481 tttattgtcc tgcacgactg agggaacaga tgaaaaagaa gtaatttttt attttgaagt
    21541 atctacctta tttgtagtga acaatttaga acaaacacaa aacgcatcta ctgttatcta
    21601 ccagtagatg cgtttaaaga gaagaacgag aatcgtccat atgagggttt ccactcctta
    21661 tgcagatttt tttaattctt gcactgcttg ttcgatcgta acacctgtcg ctacatgatt
    21721 ggtattttct ttgtcaaaaa cgacaaatag attcaaatct gtatccaagg ttactcgata
    21781 ttgtatatct ttgttataga atgtcatagc cattctcctt tctttaaata cttgctgaaa
    21841 ggcaacgaac gcgttgataa cgaaattcca tttataagta taccaccaat tgaaaggttt
    21901 tcatagttaa gaaattttaa agtcaaaagt tgcagataaa agcaaataaa caagaaaaca
    21961 aaaagtgaat caatcgcggt tattcaactt tttcttcgat tcgaagacaa tagaagacaa
    22021 tgaattttat atttcattgc attatccttg tgttattaga aaaagcatga tatattaaat
    22081 gagtaatcta ttttgaaacg taatttgagc gatatatcta catagataaa actccttttg
    22141 cctatcaaat atttattgca acgaaaaaac gtacattgat acgtatcagg aggaatttat
    22201 ttatgaataa cggtacagta aaatggttca acgcagacaa aggttttggc tttatcactg
    22261 gtgaagatgg aaatgatgta tttgcacatt tttcagctat ccaaggtgac ggcttcaaaa
    22321 ctttagaaga aggccaagca gtgtcttacg atgttgaaga tggtcaacgt ggccctcaag
    22381 caacaaacat tgttaaagct taataaaaat tgacttttaa ccgtcttttc gaaagaaaag
    22441 acggtttttt tgtacgaaaa aatt
//
LOCUS       DANKEF010000042        22432 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000042 DANKEF010000000
VERSION     DANKEF010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22432
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.042"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(754..1989)
                     /locus_tag="PWZ48_001863"
     CDS             complement(754..1989)
                     /locus_tag="PWZ48_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HDL2261518.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            2556..3384
                     /locus_tag="PWZ48_001864"
                     /pseudo
     CDS             2556..3384
                     /locus_tag="PWZ48_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(3657..4427)
                     /locus_tag="PWZ48_001865"
     CDS             complement(3657..4427)
                     /locus_tag="PWZ48_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2261519.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            4645..5712
                     /gene="ulaG"
                     /locus_tag="PWZ48_001866"
     CDS             4645..5712
                     /gene="ulaG"
                     /locus_tag="PWZ48_001866"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDL2261520.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADSKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            5730..6191
                     /locus_tag="PWZ48_001867"
     CDS             5730..6191
                     /locus_tag="PWZ48_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2261521.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGEDEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            6211..7695
                     /locus_tag="PWZ48_001868"
     CDS             6211..7695
                     /locus_tag="PWZ48_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDL2261522.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQVLIFMGLILGCYWAVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPEGYFLIVEDYDTYAEKQRV"
     gene            7738..8037
                     /locus_tag="PWZ48_001869"
     CDS             7738..8037
                     /locus_tag="PWZ48_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2261523.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKEVTVIGIKNVMSAKEISEKFAETKWYQKNK"
     gene            8052..8690
                     /locus_tag="PWZ48_001870"
     CDS             8052..8690
                     /locus_tag="PWZ48_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDL2261524.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            8695..9555
                     /locus_tag="PWZ48_001871"
     CDS             8695..9555
                     /locus_tag="PWZ48_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDL2261525.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            9545..10255
                     /locus_tag="PWZ48_001872"
     CDS             9545..10255
                     /locus_tag="PWZ48_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDL2261526.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            10487..10921
                     /locus_tag="PWZ48_001873"
     CDS             10487..10921
                     /locus_tag="PWZ48_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2261527.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(11018..11317)
                     /locus_tag="PWZ48_001874"
     CDS             complement(11018..11317)
                     /locus_tag="PWZ48_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261528.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(11586..11780)
                     /locus_tag="PWZ48_001875"
     CDS             complement(11586..11780)
                     /locus_tag="PWZ48_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261529.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            complement(12008..12769)
                     /locus_tag="PWZ48_001876"
     CDS             complement(12008..12769)
                     /locus_tag="PWZ48_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDL2261530.1"
                     /translation="MHTPEEIMELSIHLGTKKIHKPILAKLILGFIGGAMISLGYLAY
                     VRVSASIPESMASLTSLVGASVFPIGLLVILLGGGELITGNMMAVAIAFMDKKVTFSQ
                     LVKNWSVITFANIIGAIFVAYFFGHFVGLTHSGVYLDQVIHIADHKIAVSWWQALGSG
                     IGCNWFVGLALWLSYGAKDAAGKVLTIWFPVMIFVAIGFQHSVANCFVIPAAIFEGQA
                     TWLEFLSNFIPVYIGNIIGGAIFVSGFYYVSYKHH"
     gene            13089..13334
                     /locus_tag="PWZ48_001877"
     CDS             13089..13334
                     /locus_tag="PWZ48_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261531.1"
                     /translation="MSKSKKNITIIGLGLTVYLFTEFVYRHVNLFAEPLFVLYESNSS
                     IVSELANLMIALLFTPALRVITILVCLVLLFFVNKKK"
     gene            complement(13518..14267)
                     /locus_tag="PWZ48_001878"
     CDS             complement(13518..14267)
                     /locus_tag="PWZ48_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010743713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDL2261532.1"
                     /translation="MLKLERHQKILDLLNAEQKVIASDLSQRFNVSEDTIRRDLKDLD
                     SQGLLKRVHSGALRVGPPITNFNYRETVASEQKTALAHKALPFLKEESVIIIDGSTTN
                     LALAKEIPQDFRATIITNSPPIAIALAQHPNLEIINIGGTFYKQSMINIGVQAYKQLQ
                     NIRADLYIMGIYNIDLDVGTSVPTMQESDIKREMIRISTEVLGMITNDKFGTISNHIV
                     GPIEDLTYLISEEIPEKVRKEYNKQKIVLID"
     gene            14453..15157
                     /locus_tag="PWZ48_001879"
     CDS             14453..15157
                     /locus_tag="PWZ48_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDL2261533.1"
                     /translation="MEKKIILSPSLMCGDLANLEGSIQELESIGMDSLHIDVIDGSFS
                     PSMPLGIETIKRIREITSMNFDVHIMAKDNDNEFFIGEMLKIGVESITFHYETSLHID
                     RYIQLIKNSGTKVGIALNPATPLNVLQYILPEIDRICLMLINPGFATNKSEKQVSYAL
                     KKITDLCQLVNEKELAVDIQVDGRVSLETIPGLLDAGATNLVLGSTSLFLKGNTLAEN
                     QKLLYQAMKQSVRKNQ"
     gene            15197..15754
                     /locus_tag="PWZ48_001880"
     CDS             15197..15754
                     /locus_tag="PWZ48_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2261534.1"
                     /translation="MSYKAAKQNIVREITETLDRISSKDVEQLIKKIEKAERVFFVGV
                     GRVLLALEGMAKRLGHLGIQTVVVGQITEPAITDKDLLIVGSGSGESAFPLTIAKKAK
                     EFGSQVAHIGSNPDSTMKEYSDLFIRIPVQTKLALPEEISSIQPMTSLFEQSVLLLGD
                     SIALVMIEEKAIDMSKLWQYHANLE"
     gene            15762..16901
                     /locus_tag="PWZ48_001881"
     CDS             15762..16901
                     /locus_tag="PWZ48_001881"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDL2261535.1"
                     /translation="MKAVHFGAGNIGRGLIGQILHQNGFDLCFIDTNPELIHQLNQDK
                     GYTIEIIDEMNRSDFIDHVVALNSLSELEEVITATKNADILTTSVGAINLVKIAPIIA
                     QALSKRFEQKHSINIVANENVINASDVLKKEIYSLLSEKEQENFDEYAYFVNTAIDRQ
                     SLGKVLGGKMISVVEPYYEWIMDRQQLDPTTSFDMKNVNLVDDMEPYIERKLYIVNAA
                     HAAVAYLGALKGYKTIQEAIQNMEIFNLVKQFLMENIEYFVQKYHFSREELTSFVEKT
                     LNRHGNQKLSDEITRVGSSPIRKLGPNDRLIAPIVKLDSYHLPYDAGIKVIAAGYHYR
                     NLADPEAEQLRRMIVNDGLKATIKKISRLEGKLLNEVVAAYESQI"
     gene            16913..17344
                     /locus_tag="PWZ48_001882"
     CDS             16913..17344
                     /locus_tag="PWZ48_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2261536.1"
                     /translation="MLKEQLSGNTMFMDAIDSWQEAIRVSARPLIGKGIIEESYVQAM
                     IQNVLDNGNYIILLPLVAMPHARPEFGSKGVGMSFLCLDKPVMFPSEEPVKFFFTFSS
                     DSPKGHLDLMANLGELLSDEKMYQKLFDVKTEKALLDLIKK"
     gene            17358..18803
                     /locus_tag="PWZ48_001883"
     CDS             17358..18803
                     /locus_tag="PWZ48_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDL2261537.1"
                     /translation="MEVANKKKRVSMRAKVQAIGGFLTNMVLPNIGAFIAWGIVTALF
                     IPTGWFPNESLNELVSPTVSYLLPLLLAYTGGRMVGGTRGAVLGTIGTIGLIIGADIP
                     MFMGAMIMGPLGGWMMKKTDDFLDGKVPAGFEMVVNNFSIGIIGFILMCLSYLVVGPI
                     IQSANQFVTSAIEALVSTGFLPILSLINEPAKVLFLNNVIDQGVYYPLGMQQTLEAGR
                     SIYFMVASNPGPGLGLLLAYSLFGKKAAKQTAPGAIIIHFFGGIHEIYFPYVLMKPLT
                     ILSMIAGGMTGIITFELFDAGLVAGPSPGSIFSYLALTPRGNFLGVMAGVVTATLVSF
                     FVTSVILKADKSTDEDRNFELSVQKSKTMKSEGRKLLKEENAREICTEKKEMNKISFA
                     CDAGMGSSAMGATIFKKKLQKAGITDVEIKNYRIEEVPEDSDLVIVHQDLEERTRKAH
                     PNMKIVTLKNYLQDPVINGLAEEIIGLKTED"
     gene            19089..20714
                     /locus_tag="PWZ48_001884"
     CDS             19089..20714
                     /locus_tag="PWZ48_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDL2261538.1"
                     /translation="MKKIYSKEIDCFRGIAVLAVIFYHLPNSLLPGGLQGVTIFFVVS
                     GFLMAKNTIRDEKNRQFSFLHFYYKRIKRIYPFLLFSLVVFIFILGISNIRLIGNIRS
                     ELPSLIFGYNNWWQLNQGVSYFDSYFNASLFKHYWSLSVELQFYLIWPFLFIVIKRMR
                     RKQVFYLIYTLIFVSILFSLFLPSTKAYYHTIAKLFPFLLGVWGYFNRITIGRFFEQN
                     TFSKIWLLLLASLCLILFPIFPYTLNELLISICFALLLASVDDMNIAKKTKIASTKLS
                     FVGKISYELYLVHFPVLIALTNLFKHHHWKYLDIAGLFTVFLCTLLMITCFRKGVQKM
                     RSPKIWTAMALMIIGVSVLVLRAPASRLTADQNQLQSLLKENSSKANAGENLLSQEKK
                     ILFIGDSVMLGAYQELKETFKKEALVDAKESRQITALPEILKEHKDLVSYQQIVIGLG
                     TNGVITDEAIEQTMSLLKDKKVYWVNIKAPTGWQDTINATLATLPNQYSNITMIDWYS
                     ESKNHPEFFYDDETHLNESGRTAYAKYIASVIQ"
     gene            21346..21699
                     /locus_tag="PWZ48_001885"
     CDS             21346..21699
                     /locus_tag="PWZ48_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261539.1"
                     /translation="MNSFKKKFVSRFLNMKNFFEKKGDYDAYNYVLNLEKQTYYTLRK
                     NDDNDFWTMLPKMLSIDSKLSLLEEFINLDIEEFCGGKELINMVESDYHTYYKESCGY
                     KVDEQCPQSIIFYIE"
BASE COUNT         7545 a         3401 c         4415 g         7071 t
ORIGIN      
        1 aaaaatgaaa attttatcag caatggatta agaattagtc gatctactac caaagtgatc
       61 aaggcaaata tagatttggg agcttacaac catatagtag gggctgcagt atggtgtatg
      121 aatggtaata tgtatgatga gagtgtttat tcaaatcatg aaacgtgtat agaaataatt
      181 gcgctgggaa tagcaatcag tcatggtgaa cgtgactttt agtttatcgt tgctgacgac
      241 atagaacatt ctgacatgat ctactcagca tgtggggatt gctggcagtt tatataggag
      301 tatgcatcgg attgcgagat cattgtctca acagaagagg gaggttgatt atcaaagata
      361 aagtggtaaa tcctacattt gatttagtaa ttgttgaagg gactagcagc acaaatctct
      421 acaaagcgat gcttctagat ctatatgaca cattttaaga aaatgtattt gcctaattat
      481 tatggtcttt cttttgaaga aacgttgaga tgatccaacc agagaaaggt gtcaaagaat
      541 ttgactaaga ataaatggca gacgctacct atgctcattt ggattgcgtc agatctttta
      601 ttttaaggat gattttgtgt tttttagaca ccgtaattta agtaactcta acttttcaat
      661 ccactattta ctttttttgt attttcactg tctgggtctc gaatagcgaa attactgatg
      721 agtctctatt aaaaaagctt tttctacttg aaactatatt gggtggttgc tcaggaaggt
      781 ttcgatttct tgaattgctt gttgtggacc atttattgga aaaacggctt gcagcgtgcg
      841 tgcctgctga cgaatcttgg gtgaagaaac ttcgcgcaga gcaatctgca gttttctttc
      901 agtcactggt tttgtgaggg cgattgcgtt tccgtatttt acacaatagt caatattaca
      961 gctttgctca aattgcatcc ccacaccgat aaacggtttg ccggattcac aggcagtttg
     1021 gacggtccct tccccgccgt ggataaaaga aaaatcaatg aattcatgca aaagatgact
     1081 agacagatat tctgttatta aaagatcttc tgagtcagat tgaatgtcat caagcagata
     1141 gtcaataggt gcaataaccg ctagatccgg ttgtttttgt aagaagaaca aaattttgcg
     1201 taacatacgc ggattgcctg aactacccat tgcgaaatag ataatacgtt ttctttcttg
     1261 ttgctttcgc tgtataaaag ttagcacttc ttcaggcaag gaagtactga gcttagcaaa
     1321 aattggacca acgattcgat aattttccgg cagctgttct tcttccaaaa atagtttagg
     1381 cgttgtgatg agatttagat cagcatccat catttgtaaa cttgtttttg gtaaaggaag
     1441 catttctttt tgtgcagcgt gtttgaaggc attggggatc catcgggtat ttactatacg
     1501 cttttttgtc cagtgccaaa ggggattgct taaagggccg aagaaacgta actcgtgggg
     1561 catcagcgca tgctctgatt gaaaaaaagg ttcactcaaa gcatagggtt taacatatac
     1621 taaaggaatc tttttattgc gggccgataa aaaaattgtc acattcgaac ctgtcacaat
     1681 cagtcgaggc tcaaacttag caatcagact ctgttcacta gtgatacgag aggcaaccat
     1741 ttctttggta aaagggtttt taagtgtacg caactggtca aattttatga tttgttcttc
     1801 ttgttttttc gtaaggatcg gcgctagtaa atgatactca aagccacttt ctgtgatcaa
     1861 aggagcaaaa gttttagaaa agccaaagaa tacacagtaa taacggtctt tcatagcttt
     1921 ggcaacctca atcattcgtg ttgtctcagc taaattgaat actgcaggag caaaaagaat
     1981 tgtcttcatg aaatcctcct tattggaatg agtctatccc ttcgtttctt actagtatac
     2041 taaaaaacac agcgttcgag tagcctatgt atattttgaa tattaaactg tctctcgctg
     2101 ttttcacaac ttctaaattt aaattttgta atcaagttga aactccaatt ttcaaaaaaa
     2161 cacgcaacaa ttttttgatc gatttatatg ttgtgccaga taaatacgag aagttgtgtc
     2221 attaaaaata aacatgagaa cataaaaaat aaaataatta tattaattga taggtgtgct
     2281 gtcgtcatta tacacacgta agaaagaaat agttatgttt aaaaatattc ttagactttc
     2341 tcgccatgac aattttcact atgtgttata actgtaaacg ataattattc agataacaca
     2401 aaaagaaatc aaggagcaga tacgccagtt gtttaatttg aatattattt taaaattaaa
     2461 atttttattc tgttctaaac atttttggtt gtttagaact tttttttgtg tatgatataa
     2521 aggagaaata attttttaaa gggaaggaaa ttgttttgat taaagtagat ggagcattac
     2581 taaaagaata ccgaaagaaa aaggggttaa cacaagtaca attagcagaa ggcatttgtg
     2641 aacaagcaac tgtcagcaat atcgaaaaca agaatatgtc gccaggcatc aatatcttaa
     2701 gtgcgctttg caaaaagctg aatgtcgaga tctccgatat attagtagaa acagaagaag
     2761 acaaaatcag gaaaaaaata aatgagattc aatcattgaa ttctgtatca aaataccaag
     2821 aatcttataa aattttacaa acgatcgatc aggaaaaagt aagtaattct gatcttttaa
     2881 atcaaatttt attttatcaa ggactgcttg atcatcttta tgcgaaaaaa accagatgca
     2941 gcattatttt attttaatca agtgttggaa caaacgaccg aaacagatat ccatcactta
     3001 caagcagctg caaacgtggc aatgatctac ttgtcaaaag gagaattaga ttttgcgaaa
     3061 gtttatgtgg agcgtacact taaaatattg agtgagactg attttgataa tctgatggtc
     3121 tgcattgtat attatgatat cgccacctat taccgtaaaa tcgaggatta tgataaagca
     3181 atccaattat gtgagaaggg tattgagtat aataagaaac ataaatcgac ttatgcattg
     3241 gaatacttgc tttatgaaat tgcttcttgc cataagcaat taggagaaga tgattattta
     3301 gaaagatata tggacgcaaa aaaaatagcg cgtttcaacg gaaatgatta cgcggtaaag
     3361 gtgattgaaa acgacctgaa ataaagtggt tgtgaaatca gtgttctgat ttgaataccg
     3421 tttattcgaa aaaaggaggt gtgacttttg tcatacctcc ttttttcgat attgtttgat
     3481 tcctatttag gcaacatata acggttataa aagaagcaat agattatgat ggtatcgatt
     3541 ggatggacac atcttgggat tcctttatca atttgagcgt agcgtgtttt tcatttatcg
     3601 ataaatttgt tattcatttt tttgataatc ctatttacat atttacgtcc tggttcttaa
     3661 aatgaatcta cttgaatggt ttttatcccc gtatttctga tctcctctaa tactttttca
     3721 gaacaaaatt tatcagtgat caaataatca aagactgaaa tatcagcaat cttgaacttt
     3781 gatgtcagtc cgatttttcg atgatcagcg actaaaatct tacatttgat tgtcttgttg
     3841 accatcattt cgttgatcgt tgattcattt aatatctttg ttgtgacccc atgtactaca
     3901 tcgactccgc tacagccaat gatacaaatg tctgcattga ctgaagaaat cgtatttaat
     3961 gcgatgtcgc ccactagtgc ttcttttgga aagcgcatct ctccaccagt taaaatataa
     4021 ctacaattat ctttgtgcgg agagtaattg attttcacgt tgtttgtaat gactgtcaaa
     4081 tggttagatt gaagaaaatc cagtgttttc aacgcagtcg agctagaatt gataaacagc
     4141 gtatcataat cgttaatgta tttactagct tctctggcaa tcgcttgttt aatcctttca
     4201 ggaccagatt gatcaaaatc agtctggttt ttgtaattat aagaacaata accgtaattg
     4261 cgtatgatct cgtttttttc ttctaataaa tttaaatctc ttcgtatcgt actaatagat
     4321 acatcaaggg attcagctaa ttcgaaagtt gatagttgtt cagtctgctg aagcagttcc
     4381 aaaatttctt tttgacgttt ttcaatattg acaagtgagt ttttcatgac ttttgcctcc
     4441 tgagggacat cttgatccat ggatttattt gactagttgt tcatttaaac attcatatct
     4501 gtctttatta tactgaaaca atcattgatt actgaaagaa aatcatataa aaaacaaacg
     4561 gtcatataaa agggtttcca tggatgctga tacacattaa tatttacttg taagttggaa
     4621 caacgatatg gggggagcaa gcaaatgagt aaaactgtca atgaagtaac aaaagaaaag
     4681 tggttagtag aaacatttcc tgagtggggc acatacttga atgaagaaat cgaaacagaa
     4741 gtagtggatc ctggaacttt tgctatgtgg tggctgggct gtacgggaat ttggctgaaa
     4801 agtcatgaag gaacgaatat tttatgtgat ctatggtgtg ggacgggaaa acgtagtcat
     4861 gggaatggca tgatgaaaaa aggccatcag atgatgcgta tgagtggcgt tcaaaatatg
     4921 cagcctaatt tacgtactca gcctttcgta atcgatcctt ttgcgataaa aaatgtagac
     4981 gctttaatcg ttacacatat ccattccgat catctagata tccatacagc cgcagcagtt
     5041 catcaaaatt gtcctgaagc aacattcatt gggccaaaag aagtggttcg cacgtggcaa
     5101 aaatggggaa ttcctgaaga aaaaacgatt acagtaaagc caggtgatgc agtcaaaatc
     5161 aaagacatca atgtagtagc tttagaagcg tttgatcgga cagcgttggt cacttgtgaa
     5221 gatccagaag aggtattaaa aggaaaaatg cctcaggaga tggatcaaat tgcagtaaac
     5281 tatttattcg aaacatcagg agggaatctt tatcatgcag gagattcaca ttactccaat
     5341 ttatttgcca aacatggtaa tgaacacaaa atcgatgtct gtttaggggc ctatggtgaa
     5401 aacccacgag ggataacaga caaagttact tctgttgata tgctgcgcat ggcggagtca
     5461 ttgaatgcaa aagtagtgat tcctgtccac tacgatattt ggtctaattt tatggctgat
     5521 tcaaaagaga tcactgaaat ttggaaattc aaaaaggatc gcctgaagta tggatttaaa
     5581 ccttatatct ggcaagtagg cgggaaattc atctatccga caaacaaaga tgatcttgag
     5641 tttaattttt atcgaggatt tgaagatgtc ttctcgattg aaaatgatac accgtttccg
     5701 tcgtttttat aagaaagaag gggagtttct tgctgaaata cttttttgac catcaattga
     5761 ttcattaccc agataaagaa atcacggatt ggcgtgaagc tatcgccgaa agctgtcttg
     5821 tattgatcga taaggaaatt attgatgaaa cgtatgtgga ggagatcatc cattgtgtag
     5881 aaaaatacgg tccttatatt gtgatcgcac ctcaagtagc aatgccgcat tcttcagaaa
     5941 aaagtgatgg tgtttttgga acagctattt catttaccaa gttgaaacag ccaatccgtt
     6001 ttgaaagtga aggggaggat gaggagaaaa cggctagttt gttttttaca cttgcagcaa
     6061 aagatccaga agaacatcta gaaaatatcc aaaaattatc cgaattatta atgacagatg
     6121 gattggttga gcgtctcatc tctacgaact ccatcgatga ttaccaagtt gtgatggcag
     6181 aatataatta attgatagag gagaaaaaag atgggggaat ttttgttaag catttggacg
     6241 tattttgcaa caaatatctt gacgcagcca gcatacctga ttggttttat cgtgttatta
     6301 ggctatattt tgctgaaacg cccgcttcat gaatgcatcg ctggattctt aaaggcaacg
     6361 gtcggttact ttattttgac tgttggctct ggtggtttgg tcaataattt tcggccgatt
     6421 ttggttggac taaaggaacg gttcaatttg gatgccatgg ttacagatcc ttattttgga
     6481 caaaatgctg tggatgctgg attaatggag acatttggcc gtacctttgg agatgtgatg
     6541 attttattac tgattgcatt tgttttaaat atcttattag tccgttttca gaaatatacg
     6601 aaactgcgtt ccgttttcac taccggaaac gttcaggttc aacaagcagc taccgctttt
     6661 tggattttac tgttttgctt tcctgatcta ggtaggatac aagtattgat ttttatggga
     6721 ctgattttag gttgttattg ggcagtagct tctaacttaa cagtaggaat cacgcaggaa
     6781 ttgacagaag gtgccggatt tgcagtggca catcagcaaa tgttcggaat ctttatattt
     6841 gcaaaattag ctgagtggat gaaaaagcgg gatgaaaaga aaaatagatc aatcaagcaa
     6901 gacaaaaaaa tcgaagatat caaattacca ggattcttgt cgattttcaa tgagaatatg
     6961 gttgctactt cgttactcat gctatttttc tttgggatca ttctgattgt actaggcaaa
     7021 gattatttga ttcaagcgca attcatgcaa gaaggacaga gtttcttatt ctacattatg
     7081 acgacttctt taaattttgc ggtatatctg gcgatcttgc aattaggtgt tcggacattc
     7141 gtcgatgaac taactcaatc tttccaagga atctcaaaca caattttacc aggagctgtt
     7201 ccaggaattg acgtagctgc tacttttggc tttggttcgc caaatgcggt aacgattggg
     7261 ttcttgtttg gtgctttggg acagtttttg atgattggtt tactgatttt attgaaatca
     7321 ccaacgatcg tcatagccgg tttcattccg ttgttctttg ataatgccgt tatagcagtt
     7381 tatgccaata accgaggtgg attcaaagcc gcttgcctgt tcccgtttct ttcaggaatc
     7441 atacaagtag gcggttcagc attattcgct acatggattg ggatgtcaac atatggtggt
     7501 tatatcggga tgttcgactg ggcaacagct tggccattgt ttacagtaat catgaaagtc
     7561 ctaggttatg ctggaattgc cgtagttgtc attggtttat tggctattcc gcagttacaa
     7621 tatcggaaaa atcctgaggg ttattttttg attgtagaag actatgacac atatgcagaa
     7681 aagcagagag tataaaagaa aatctgatta gacaacactc ataatggagg gattaagatg
     7741 agaattttag tatcgtgtgc aaatggatca ggaacaagct tgatgatgat gagaagtgtg
     7801 gaaaaagcaa tgaaagaact aaacgtacca attaccaaaa tccatcattg tgccatttca
     7861 gagggaaaaa gttcagctgg ccaatacgat gtagttttta cgccagtcaa ttttttgaat
     7921 atgtttactc aggcagagaa aaaagaagtt acagttatcg gtatcaaaaa tgtgatgtca
     7981 gccaaagaaa tcagtgaaaa atttgctgaa acaaagtggt accaaaaaaa caagtaaagg
     8041 aaggaactaa gatgagcagg ccgaatttgc aaattgcgtt agatcacaat gacttagaac
     8101 atgcattagg agatgttatg aaagttggtg atgtagtaga tattattgaa gtgggcacaa
     8161 ttctttgctt acaggaagga caaaaagcga ttcgctgtat tcgcagtatg ttcccagata
     8221 aaaaactagt cgctgatacg aaatgtgcag acgctggagg aacagtggcg agtaatgttg
     8281 caaaagcagg agcagactgg atgacagtca tttgttgtgc aacgattcca actatggagg
     8341 cagcacaaaa agaaataggt gaattgcaag tagaactcta cggcaattgg acttttgaac
     8401 aagccatgga ttggcataac atcggtatcc ggcaggtaat ctaccatcaa agccgtgatg
     8461 cgctgttatc tggtgaaact tggggtgaaa aagatttatc gaagatcaaa aaactgattg
     8521 aactaggctt taatgtttct gtaactggtg gattaaatcc acacacatta cacttatttg
     8581 aagggatcga tgtgtatacc tttatcactg gtcgtggtat tacagcagca aatgatccga
     8641 tgaaggccgc acaaaacttc aaagatgaga tcattcgtat ctgggggtaa ggaaatggct
     8701 caaattggga tttatgaaaa agcattaccc aaagatattt catggaaaga acgtttttcc
     8761 cttgtaaaag aaatggactt cgattttatc gagatgtcga ttgatgagac ggatgagcgg
     8821 ttagcacgat tagattggtc tgaagaaaag atagctgagc tgagagaaga gatgttcagc
     8881 tccggtgtac gtatccattc gatttgttta agtgcgcatc ggcgatttcc gtttggttct
     8941 gctgatccag aaaagaaaaa agcagcgaaa caactaatga aaaaagcaat ccatttggcg
     9001 cataatctaa gcgtcaaagt aatccaaata gctggatacg atgtttatta tgaagagaaa
     9061 tcaatgaatt ccagagaaga ctttatcgaa ggaataaaag aaagcgtaaa ggaagcttct
     9121 aaatatggca tcattctttc cgtggaaatc atggatgatc catttatgaa ctccatttca
     9181 aaatttttag aaatcaaaga acagattcat tccccttttc tacaagtata tccagaccta
     9241 ggcaatttgt ccgcatggcc ggaaaataat ccggctcgag aacttgagaa agggatcgat
     9301 tatatcactg ctatccactt aaaagatact taccctgtaa caaaagaata ttcaggccag
     9361 tttcgtgatg ttccttttgg tagcggttgt gttgattttc ttggattatt aagacatttg
     9421 aaacgtctag attatgatgg aacatttctg attgaaatgt ggagtgaaaa aagccaagac
     9481 tttcgaagag aaatccagca ggcaaaagac tacttaagtt ctaaattgaa ggaggctggc
     9541 tatgatgtct tgtgaagaaa aaattgcagc aatgaagcaa cgtgtccttg aagcaaatct
     9601 ggcattgccg cgatacggat tagtcaaatt gacttggggc aatgtgagtg aagtggaccg
     9661 tgagctagga gtcatcgtca ttaagccaag cggtgtcaga tatgaatgca tgcaagcaga
     9721 tcaaatggtg gtaacagatt tgtcgggaaa tataatagaa gaagatagct taaaaccatc
     9781 ttcagatctc cccacacatg ttgttttgta tcaaactttt gaagatataa ctgctattac
     9841 tcatactcat tccacgcact ccgtcatgtg ggcgcaagca ggacgcgatt tgccagctta
     9901 tggcacgact catgctgatg ctttttatgg taaggttcct tgtacacgcc aattaacgaa
     9961 agaagaagta agagaagctt acgaagttca tacagggaat gtgattgtcg agacgtttaa
    10021 ggaaagaaaa ttagatccaa acgaagtccc tggagtactt gtctatggac atggaccgtt
    10081 cacatggggg gattcaccta tgaaagcagt agaaaacagt ttgatcttag atgaaatttg
    10141 tctgatggcc aaagaaaacg agctgatcaa ccccaatata tgtgaaatcc cgcaatattt
    10201 actagataaa cattattttc gcaaacatgg aaagtatgca tactacgggc aatagagatg
    10261 ctagggtaag ggtagaaaat taaaagataa cacttgatag agagaaacag aggttgtgaa
    10321 gaaaatttat cacaaccttt ttatttatca aaaaggatgc attctaaaag gaaagagaga
    10381 ttttaaggaa tcgagagaat ctttacagga gagttatatg tgatagatta aatagtcaaa
    10441 gaactgtatt attagaggga gaattgtata agaaaggaag aaaaaaatga atattgattc
    10501 ccttatgatc gatgagattt ctgaattata taagagcgtt ggttggacga attacacaaa
    10561 agacactgca aggttagcta gagcatttga acaatctgaa tctctgataa agcgtaatgg
    10621 ggaagggaag attatcggaa tcgttcgttg gatcactgat tttgcgacga tcgcatttat
    10681 tcaagatatc ctcattcatc cgaggtatca gcgccaagga ataggcaaag tgcttttgaa
    10741 tgaagctttg gagaagatca ctagttatgg accagtccaa atcgaattat tgacagatga
    10801 tactgagaaa acaaaaaaat tttatgaatc agtaggattt gttcaagtaa aggaaatggg
    10861 tggtatttct tatgtcatag atactagaag aaaaaaggtg ctttttggta atgacaaata
    10921 acatataata acgtacaaaa gaaaaaattc tagcttagtt ctttagaaaa acaagaggat
    10981 agacttagta tcactcaatc tatcctccaa tgctcagcta ttttattttt ttactcacct
    11041 ctttatcaaa tacaattttt tgcaaattcg aactaatata aaaaatgaac acatgatttt
    11101 tttttaaagc aaatgctatg cgataatttt ttttattaat tttgactttc atctcgtata
    11161 ttccttttgc cgaacgagta cgcactggct ttatacgaaa aaaattatgc tccaaatact
    11221 ctgtcacttt gtgggaaatt gcttctttat acatgaaatg ttcttcttgt ggaaattgct
    11281 taagtagttc actgtcaaat tcaacagtat aatccatgtt ttatttcctt tctatactga
    11341 tacattataa aaataaacaa ttttataagt ctctatattt catgatgaat gacaagtata
    11401 tttgattcat cttgatatag ctctacgaga tctccaattc caggatacat attgaaataa
    11461 tcatatggta tacaaacatt tatttttttt caattgtcaa cactgcgtct tcttcttcta
    11521 attctataat cttagcaatt tgtacgtcgc ttttcatgtg tgatcttcct ttctatacat
    11581 tggatttact tttcgatgat atctacaaca gctttgccac aagtcagttc tttttctagc
    11641 tcgttcttta tttttatggc acatggtttg gaaaaaggag cagcttcttt ccatgaacca
    11701 agtaaaggat attcgttact gatacctttc acatattgtt tgtttttgac gccttttgtt
    11761 tttacttcta cataatacat ttgatcctct tctttcttgc tgtttttagt taatattcaa
    11821 atatatgtaa gtgaaaatga ttatgtatct atatctttat gataaataaa aagaatcaat
    11881 ttgaaagttt ttttcactcg atataactaa tttgaataat atatataata aaaggctatt
    11941 gttcttaaaa tataaatagt atataaagag attgaaggta atgctcgaat gaacattacc
    12001 ttcaatctta atgatgctta taactaacgt aataaaaacc tgaaacaaaa atagctccgc
    12061 cgatgatatt tccaatataa acggggataa aattcgagag aaattctaac caagtcgctt
    12121 gaccttcaaa tatggcagca ggtatcacaa aacagttggc cacactatgc tggaaaccga
    12181 ttgccacaaa aatcatgact ggaaaccaaa tcgttagtac tttgccggcc gcatcttttg
    12241 ctccatatga taaccataac gctaaaccga caaaccaatt acaacctatt ccggagccta
    12301 atgcttgcca ccaagaaaca gcgattttat gatcagcaat atgaatcact tggtcgagat
    12361 agacacctga atgagttaaa ccaacaaagt gtccaaagaa ataggcaacg aaaattgcac
    12421 caataatatt tgcaaaagta atcactgacc aattcttaac cagctgtgag aatgtgactt
    12481 ttttatccat aaaggcaatc gccacagcca tcatatttcc ggtgatcaat tcaccaccgc
    12541 caagaaggat gaccagcagg ccgataggaa aaaccgaggc accaactaaa ctagtcaagc
    12601 tagccataga ttctgggata gaagcagaga ctcgtacgta tgccaaataa cctagagaaa
    12661 tcatggctcc tccgataaaa cctaaaatca gtttagctaa gataggttta tgtatttttt
    12721 ttgttccaag atgaatactg agttccataa tttcttctgg tgtgtgcata atacttcgat
    12781 tcctactttc ttaatgaatt ttcatgtgat aaaatatagt gaaaaaaatc acattttctt
    12841 tgttgattat atcatcttat tatttatatg tgcatattat tgtaaaattt tctatttaat
    12901 cacatttgat tctaaaaaag aaacaaaaat aaaagtcatg tatatggtgg tcaaaaagta
    12961 aataatatca gttatcaaat ttcctgtaaa ttttcttgaa actacagcat attattactt
    13021 caaaacaatc tacatttttt ataaaaaagg aaatgaataa ctgaaaaaat tattatagga
    13081 gagaaaaaat gtcaaaatct aaaaaaaata tcaccataat tggtttaggc ttaaccgtat
    13141 atttatttac agaatttgtt tatagacatg tgaatttatt tgcagaacct ttatttgtat
    13201 tatacgaatc aaattcctca atcgtcagtg aacttgctaa tttaatgata gcattgctgt
    13261 ttactcccgc gttgagagtc ataactattt tggtctgttt agtgctattg ttttttgtta
    13321 ataaaaagaa ataacgtagt atttcaaaag acaaagaagt gggatgatga ttgctatagt
    13381 atgttatacg tggtgctagg taagtgagaa taaacaaaaa aggaaacacg caataaagtt
    13441 tatttcaaaa ctttattgcg tgtttctttt ttagattttt tatatctgtt gctttagtag
    13501 ttaccaatag ttttcgctta atcaatcaaa acgatttttt gtttattgta ttctttacga
    13561 accttttcag gaatttcttc tgaaataaga taggtaaggt cttcaattgg accgacgata
    13621 tgattactga tggtgccgaa tttatcatta gttatcatcc caagtacttc agtagaaata
    13681 cgtatcatct cacgtttaat gtcggattct tgcatagttg gcacgctcgt tccaacatct
    13741 agatctatgt tataaatacc cataatataa aggtcggctc gaatgttttg tagttgctta
    13801 taagcttgta ccccaatatt gatcatagat tgtttataaa aagtaccgcc aatattgata
    13861 atttccaaat taggatgctg tgctaatgct atggcaatcg gcgggctatt ggtaatgatt
    13921 gttgcacgaa aatcttgtgg gatttctttt gctaaagcca agttagttgt acttccatca
    13981 atgatgatga cagattcttc ttttaaaaaa gggagggctt tatgagctaa tgcagttttt
    14041 tgttcgctag ctacagtttc tcggtagtta aaattggtaa ttggcggacc aacacgtaaa
    14101 gcgccgctgt gtactctttt taagagacct tggctatcca aatctttcaa atctcgtcga
    14161 atcgtgtctt cggatacatt gaaacgttga cttaaatcac tagcgataac tttttgttct
    14221 gcgtttaata aatctagaat tttttgatga cgttctaatt tcaacatgtt gcgtgctcct
    14281 tcttatttat tcttgtttat catgtcattt atgcgttttt atgtcaataa tttccgtttt
    14341 taatacacta aaaaggcata aacaagcaaa aacaaaatat ttttatgcag ttttatgttg
    14401 actagtattt gttaagcggt tacaatgagg gggtaattaa aggagggaat gtatggagaa
    14461 aaaaattatt ttgtcgcctt cacttatgtg tggggatcta gcaaatttgg aaggaagcat
    14521 ccaagaacta gaaagtattg ggatggattc cttgcatatt gacgtgattg acggttcttt
    14581 tagtcccagt atgcctttag ggatcgaaac aatcaaaaga attagagaaa tcacttcaat
    14641 gaattttgat gtacacatca tggctaagga taacgataac gaatttttta ttggagaaat
    14701 gttgaaaatt ggcgtagaga gcattacttt tcattatgaa acaagcctcc atattgatcg
    14761 atatatccag ctcatcaaga attctggaac aaaagtaggg atagctttga atccagcaac
    14821 tccgttaaat gtattacaat atattttacc cgagattgat aggatttgtt tgatgctgat
    14881 taatccaggt tttgcaacaa acaagtcaga aaaacaagtt tcttacgcgc taaaaaaaat
    14941 tacagatctt tgccagttag taaatgagaa agagttagca gtagatatcc aagtggatgg
    15001 acgggtatcg ttagaaacga tcccgggatt attagatgca ggcgcaacaa atctggtatt
    15061 aggaagtaca agtttgtttc ttaaaggaaa tacattagca gaaaatcaaa aactacttta
    15121 tcaagcgatg aagcaatcag ttaggaaaaa tcaatagatg tagtggtcct gaataataga
    15181 aatggagggg aaatagttga gttataaggc ggcgaaacaa aatattgtaa gagaaattac
    15241 tgaaacatta gaccgtattt cttcaaaaga tgtggaacaa ttgataaaga aaattgaaaa
    15301 agcggaacga gtttttttcg tgggggttgg cagagtactg ttagcgctag aaggaatggc
    15361 aaaacgctta gggcatctag gaattcagac agtagtcgta ggacaaatca ctgaaccggc
    15421 aattacagat aaagatttat tgattgttgg ttctggtagt ggggaaagtg ctttcccact
    15481 aactattgca aaaaaagcaa aggaatttgg gtcccaagtt gcccatatag gatcaaaccc
    15541 agacagcaca atgaaagaat attcagatct ttttattcgt atacccgtac aaacaaaatt
    15601 agcgttacca gaggaaattt cttctataca gccaatgact agcctgtttg aacaaagtgt
    15661 tcttttgtta ggagatagta ttgctttagt gatgatagaa gaaaaagcaa ttgatatgtc
    15721 aaagttgtgg caataccatg ctaacttaga gtaggtgaaa aatgaaagcg gtacattttg
    15781 gagcagggaa catcggcaga gggcttattg gacaaattct acatcagaat ggattcgatc
    15841 tatgttttat tgatacgaat cctgaactga tccatcaatt aaatcaagat aagggttata
    15901 ctatcgaaat tattgatgaa atgaacagga gtgatttcat tgatcatgtt gtggcactga
    15961 atagtttatc cgagctagaa gaggtgatta cagcaacaaa aaatgcagat atcttaacaa
    16021 catctgttgg cgcaataaat ttagtaaaaa ttgcaccgat cattgcgcaa gccttaagta
    16081 agcgatttga acaaaagcat agcatcaata tcgtagctaa tgaaaatgtg ataaatgcaa
    16141 gtgatgtgtt gaaaaaagaa atttattctt tgctgtcaga aaaagaacaa gagaattttg
    16201 atgaatatgc atattttgtg aatacggcaa tcgatcgaca atctttagga aaagttcttg
    16261 gaggaaaaat gatctctgtt gtcgaacctt attatgaatg gataatggat cgacagcagt
    16321 tagatccaac tacatctttt gatatgaaaa atgtaaattt ggttgatgat atggagcctt
    16381 acattgagcg aaagctttat atcgttaatg ctgctcatgc ggcagtagct tatttaggcg
    16441 cactgaaagg atataagacg attcaagaag caatccaaaa tatggagatt ttcaatctag
    16501 tcaagcaatt tttaatggag aatatagagt actttgtaca aaaatatcat ttttctagag
    16561 aagaattaac atcttttgtg gaaaaaacat taaaccgtca tgggaatcag aaactctccg
    16621 atgaaattac tcgtgtagga agttccccta ttcgaaaatt aggccctaat gatcgtttaa
    16681 ttgctcccat agtaaaacta gattcatatc atttacctta cgatgcagga ataaaagtaa
    16741 ttgctgcggg atatcattat cggaatcttg ctgatccaga agctgaacaa ctacgtcgaa
    16801 tgattgtaaa tgatgggttg aaagcaacga ttaaaaagat ttctcgtttg gaaggtaagc
    16861 tgctgaatga agttgtcgct gcttatgagt cccagattta gaggaggaga atatgctaaa
    16921 agaacaattg tcaggaaata caatgtttat ggatgcgatc gattcatggc aagaggcaat
    16981 ccgagtaagt gcgcgtcctt tgataggaaa gggaataata gaggaaagct atgtacaagc
    17041 gatgatacaa aatgtgttgg ataacggaaa ttatattata ttactgccac tagtagcaat
    17101 gcctcatgcg cgtccagaat ttgggagcaa aggggtcggg atgagctttt tatgtttaga
    17161 taagccagtc atgttcccaa gtgaagaacc agtaaaattc ttctttacct tttcttctga
    17221 ttcgccaaag ggacatttag acttaatggc caatcttgga gaattgctga gtgatgaaaa
    17281 aatgtatcaa aaattattcg atgtgaagac agaaaaagca ttgttagatt tgataaagaa
    17341 gtaaaggaga gaagaaaatg gaggtagcaa acaaaaaaaa acgagtctcc atgcgagcta
    17401 aagttcaagc gattggcggt ttcttaacaa atatggtatt gccaaatata ggcgcattta
    17461 ttgcatgggg aattgttact gcattattca ttccaactgg gtggttccca aatgaatctc
    17521 tgaatgaact cgtaagtccg acagtcagct atctattgcc gttactactt gcctatactg
    17581 gtggtcggat ggttggtggt acacgtggag ctgttttagg tacaatcgga acaataggtt
    17641 tgattattgg tgctgacata ccaatgttca tgggagcaat gattatgggg ccacttggtg
    17701 ggtggatgat gaaaaaaaca gatgattttt tagatgggaa agttcctgct ggttttgaaa
    17761 tggtcgtcaa taatttttct ataggaatta ttggtttcat actgatgtgc ctttcttatc
    17821 tggtagttgg accaataatc caatcagcta atcaatttgt aacgagtgca attgaagcat
    17881 tagtttcaac tggtttttta ccgatactgt ccttaatcaa cgaaccggca aaagtcttat
    17941 ttttaaataa tgttattgac caaggtgttt attatccatt aggtatgcaa caaacattag
    18001 aggcaggcag atcaatttat ttcatggtag catcaaatcc tggccctggc ttaggactat
    18061 tgttagctta ttctttattt ggtaaaaagg cagcaaaaca gacagcacca ggtgcaatca
    18121 tcatccattt tttcggcgga atacatgaga tttattttcc atatgtatta atgaaaccac
    18181 tcactatatt atctatgatt gctggtggaa tgacaggaat catcactttt gaattatttg
    18241 atgcgggcct ggtagcaggt ccaagtccag gttctatttt ttcttattta gctctaacgc
    18301 ctcggggaaa ttttttgggt gtcatggcgg gagtggtcac agcaacactg gtttcctttt
    18361 ttgtaacatc agtaatttta aaagcagata aatcaacaga tgaagacaga aattttgaac
    18421 tatcagttca aaaatcgaaa acgatgaaat cagaaggaag aaaattatta aaagaagaaa
    18481 atgctagaga aatatgtacc gagaaaaaag agatgaataa aatttccttt gcttgtgatg
    18541 caggaatggg aagcagcgca atgggcgcaa caatattcaa gaaaaaattg caaaaagcag
    18601 gtattactga tgttgagata aaaaattatc gaattgaaga ggtaccagaa gatagtgatt
    18661 tggtgatcgt acatcaagat ttggaggaac ggactagaaa agcacatcct aacatgaaaa
    18721 ttgtgacatt aaagaattat ttgcaagatc cagtaatcaa tggattagca gaagaaatca
    18781 ttggattaaa aacagaagac taatttatgg gaatttttga ttaaccttta ttaaaattat
    18841 tttgcctaat ataaaaaggt tgtgacatgg cttgtgtcgc aacctttttt tgtcgcacaa
    18901 ataattaatc ggatatttta tgttcatatt ttttgtgatc aacgagaact gatggggcaa
    18961 agatacattt tagatacttt taactgttaa tttacaaaac aaggtgaaac gaatagaaaa
    19021 atgaaaaaat cgatacggaa gaatatgttt gattaaaaca tgcatgaaga gaaggaggtt
    19081 gatgatacat gaaaaaaata tattcaaagg aaatcgattg ttttagaggc attgctgttt
    19141 tagcagtgat attttatcat ttgccaaata gtttgctgcc aggcggattg caaggagtaa
    19201 cgatcttttt tgtggtttct ggttttttaa tggctaaaaa tacgataaga gatgaaaaaa
    19261 acagacaatt cagtttttta catttttact ataagcgtat caaacgtatt tatccatttt
    19321 tactgttttc tttggtcgta ttcattttca tactagggat tagcaacatt cgattgatag
    19381 gaaacattcg tagtgagcta cccagtttga tttttggcta taacaattgg tggcagctga
    19441 atcaaggagt atcttatttt gactcttact ttaatgcttc tttatttaaa cattattggt
    19501 cgctttcagt tgaactacaa ttctatttga tatggccatt tttgtttatt gtcattaagc
    19561 gtatgagaag aaaacaagtg ttttacttaa tctatacatt gatttttgtt tctattcttt
    19621 ttagtttgtt tttaccttca actaaggcat attatcatac gatagctaaa ctttttccat
    19681 ttttattagg agtttggggg tattttaatc gaataactat tggccgtttc tttgaacaaa
    19741 acacttttag taaaatctgg cttttattac tagctagtct ttgtctgatt ctgttcccga
    19801 tttttcctta tacactaaat gaactactga tcagtatttg ttttgctcta ttgttagcca
    19861 gtgtcgatga tatgaacatt gcaaaaaaga ccaaaatcgc tagtacaaaa ctttcttttg
    19921 taggaaaaat cagttatgaa ctttatttgg tgcatttccc agtattgatt gctctgacaa
    19981 atctattcaa acatcaccat tggaaatatt tggatatcgc aggtctattc actgtgtttt
    20041 tatgtacatt actcatgatt acttgcttta gaaaaggagt gcaaaagatg agatcaccca
    20101 aaatttggac agctatggca ctaatgataa tcggagtttc tgtactggta ttacgtgctc
    20161 ctgccagtcg actgaccgct gaccaaaatc agttgcaaag tttattaaaa gaaaactcta
    20221 gtaaagcaaa tgcaggagaa aatctgttgt cacaagagaa gaaaatatta tttatcggtg
    20281 attctgttat gctgggggcg tatcaggaac taaaggagac atttaaaaaa gaagcattgg
    20341 tagatgcaaa agaatcaaga caaatcactg cgttaccaga aatcttaaag gagcataaag
    20401 atttagtttc ttaccaacaa atagtgatcg ggttaggaac caatggtgtg ataacagatg
    20461 aagctataga acaaacaatg tctttgctga aagataaaaa agtatactgg gtaaatataa
    20521 aagcaccaac tggctggcaa gatacgataa atgccacatt agctacattg ccaaatcagt
    20581 attctaatat aacgatgatc gattggtata gtgagagcaa aaatcatcct gaattttttt
    20641 atgatgatga aacccatttg aatgaaagtg gacgtacagc ttatgcaaag tatattgctt
    20701 cagttattca ataaggtagt gattacgtgt gttaacctta ctttttccta ataggtgaat
    20761 caatttaaat aaaaaatact tcatatgata aacggtgttt aagaggagga gctttttaat
    20821 attcgtggta aaaaagagat gggcgaggtc gttaactgag agcttcttgg aaagaaatcg
    20881 ctttggattt atcctctttg ttatacagaa catgagcgag acgacaatac taggatgtat
    20941 gttctgatcg aagttttatg gttacaagca gcaatcagct tagataaaat taaggatcgt
    21001 aggaaccaag taaagaaggc tgttagtaaa gaaacaaacg atcaagtatg ttttactctt
    21061 aagaagatgc agtgaataaa taatagtaat ataatgctag aagggactgt gacaaaagac
    21121 gtgtcacagc cttttttgtt attttaatta tcgtccagtc tttttttata aatgattgct
    21181 taagcattaa atattaaaaa atatagagtt caaaattact gcacaattag atgaaaaatt
    21241 tgaacaacaa aaagtgtatt tttatactat aatagctttt gaatttagtt actctattca
    21301 aaagttatgc tatggacaag aaaaagaata aatagaggtg agaaaatgaa tagtttcaag
    21361 aaaaaatttg tgagtagatt tttaaatatg aaaaattttt ttgaaaaaaa gggagattat
    21421 gatgcatata attatgtatt aaatctagaa aagcaaacat attatacact acgtaaaaac
    21481 gatgataatg acttttggac tatgttacct aaaatgcttt cgattgactc taagctttcc
    21541 cttttagagg aatttattaa tttagacatt gaagaatttt gcgggggaaa agagttaatt
    21601 aatatggtag agagtgatta tcatacctat tataaagaaa gttgtggata caaagtggat
    21661 gagcaatgtc cacaatcaat tattttttac attgagtaat atgaactatt aagcaggaaa
    21721 gcaactagaa gctactgatt tagtatttac aaatataaac tatattgcta acaattattt
    21781 tctagaagga ataaaagaac taagaatcaa gacgttatct tgattcttag ttcttttaac
    21841 atggaaaaga tggataatgc actgttaatt catgatgcat catcagaaaa gttggaagga
    21901 gaagatagaa atcattaaat gatttctaca gatggtaaat gaaaaaacaa aatattagca
    21961 aaaatttgcc taaatagata gtaacacttt ctaaattaaa tacaagcgca ttcataaaat
    22021 gtttatacaa atatctatgt gataaaaatt ggatatgttc acaataaata ggaattaaaa
    22081 taacgaattt acgtttgttt ttatttacca atcaaattat cttaatgcag gaataaaaaa
    22141 ttaaattaca tattgttaat catttacatc tccattaata actcaaaatg aaaagatgtt
    22201 tttattttta ttctatattt cagaaaaaaa ttaaactaga ttataataat tcttcatgta
    22261 aaaataaaaa caattacagc tatgatgttt aagataaaac aattatgaaa tcgatatccg
    22321 tttatatact tatcataagc tagaagcaaa taactagcct atgtatgtaa gtgagattat
    22381 atttattaaa aggcttcttt tgttcgatca gttatagtcg aacgagagaa gt
//
LOCUS       DANKEF010000043        22345 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000043 DANKEF010000000
VERSION     DANKEF010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.043"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(47..1342)
                     /gene="purB"
                     /locus_tag="PWZ48_001886"
     CDS             complement(47..1342)
                     /gene="purB"
                     /locus_tag="PWZ48_001886"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDL2261540.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(1355..2479)
                     /gene="purK"
                     /locus_tag="PWZ48_001887"
     CDS             complement(1355..2479)
                     /gene="purK"
                     /locus_tag="PWZ48_001887"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDL2261541.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            complement(2472..2960)
                     /gene="purE"
                     /locus_tag="PWZ48_001888"
     CDS             complement(2472..2960)
                     /gene="purE"
                     /locus_tag="PWZ48_001888"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDL2261542.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            complement(3376..4683)
                     /locus_tag="PWZ48_001889"
     CDS             complement(3376..4683)
                     /locus_tag="PWZ48_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HDL2261543.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(4686..5267)
                     /locus_tag="PWZ48_001890"
     CDS             complement(4686..5267)
                     /locus_tag="PWZ48_001890"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDL2261544.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(5383..5480)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(5839..7131)
                     /locus_tag="PWZ48_001891"
     CDS             complement(5839..7131)
                     /locus_tag="PWZ48_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2261545.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(7128..7751)
                     /locus_tag="PWZ48_001892"
     CDS             complement(7128..7751)
                     /locus_tag="PWZ48_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261546.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(7754..8590)
                     /locus_tag="PWZ48_001893"
     CDS             complement(7754..8590)
                     /locus_tag="PWZ48_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2261547.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(8607..9494)
                     /locus_tag="PWZ48_001894"
     CDS             complement(8607..9494)
                     /locus_tag="PWZ48_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2261548.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(9510..10793)
                     /locus_tag="PWZ48_001895"
     CDS             complement(9510..10793)
                     /locus_tag="PWZ48_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDL2261549.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            complement(11476..12159)
                     /locus_tag="PWZ48_001896"
     CDS             complement(11476..12159)
                     /locus_tag="PWZ48_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2261550.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(12177..12794)
                     /locus_tag="PWZ48_001897"
     CDS             complement(12177..12794)
                     /locus_tag="PWZ48_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2261551.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(12804..13628)
                     /locus_tag="PWZ48_001898"
     CDS             complement(12804..13628)
                     /locus_tag="PWZ48_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HDL2261552.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(13621..14181)
                     /locus_tag="PWZ48_001899"
     CDS             complement(13621..14181)
                     /locus_tag="PWZ48_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261553.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            14355..15311
                     /locus_tag="PWZ48_001900"
     CDS             14355..15311
                     /locus_tag="PWZ48_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDL2261554.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMIHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(15616..16572)
                     /locus_tag="PWZ48_001901"
     CDS             complement(15616..16572)
                     /locus_tag="PWZ48_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HDL2261555.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCERLLTFIRESQEDAREHQENYWLRQPLYYETCFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILARIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            16677..17279
                     /locus_tag="PWZ48_001902"
     CDS             16677..17279
                     /locus_tag="PWZ48_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2261556.1"
                     /translation="MQFGRFRLGMRTIKSAIAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(17367..18866)
                     /locus_tag="PWZ48_001903"
     CDS             complement(17367..18866)
                     /locus_tag="PWZ48_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDL2261557.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMNEE"
     gene            complement(18882..19406)
                     /locus_tag="PWZ48_001904"
     CDS             complement(18882..19406)
                     /locus_tag="PWZ48_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDL2261558.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            19581..21230
                     /locus_tag="PWZ48_001905"
     CDS             19581..21230
                     /locus_tag="PWZ48_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HDL2261559.1"
                     /translation="MFPFFKKKPLKLQNTIILVVCFSTFVSLIVSAILIRNFILSMNR
                     SSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIMIASNLDFIVVMDPEGIR
                     YSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFRYFVPIYYNQEIIGAVCV
                     GLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMKKVLLGLEPQEIAEQLSE
                     KTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDFEVGKKISDNLYDVLFKE
                     TIEHQTTVRTQSMYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGTKQ
                     YINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVPAI
                     AGFLLGKINEAKEQSVEMLLETDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQKHE
                     RNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLIHS
                     IVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            21199..21915
                     /locus_tag="PWZ48_001906"
     CDS             21199..21915
                     /locus_tag="PWZ48_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HDL2261560.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     LKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(22017..22304)
                     /locus_tag="PWZ48_001907"
                     /pseudo
     CDS             complement(22017..22304)
                     /locus_tag="PWZ48_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
BASE COUNT         6579 a         4820 c         3910 g         7036 t
ORIGIN      
        1 tatttttgct attctatttt aattgaattt tgcaactcac tcttctttat tccaaaccta
       61 ctcgtttgaa tatcacgtca acgtttttca aatgatgatg ataatcaaag gcatcgtcta
      121 tttcttcttt agataatacc gatgtgatgg ttggatcttc ttctaacagc gggcggaact
      181 gcacttggtt gtcccaagcg tacgcagttt ttggctgaac aagatcataa gcttcttctc
      241 ttgtcattcc cttatcgatc aatttcagca taacgcgctg gctataaatc aaaccaaatg
      301 ttgcagacat attccgcttc atgttttcag gaaagaccgt tagattttta acgatattgc
      361 caaaacgatt taacatgtaa ttcaataaaa tcgtggtatc aggcaagatg attcgttctg
      421 ctgaagaatg agaaatatct ctttcatgcc ataatgtgac attttcatag gcagtgacca
      481 tgtgtccgcg caccactctg gctagaccag ccatattttc tgaaccgatc ggattccttt
      541 tgtgcggcat agcagaagaa cctttttgac ctttcgcaaa aaattcttct acttcacgtg
      601 tttcggattt ttgcaatccg cgtatctctg ttgcaaaacg ttcaatactt gtagcaataa
      661 gcgccatcgc tgagaaatat tcagcatgta gatcccgagg gagaacttga gtagagattt
      721 cttgtggacg gatccccaat tgtttacata catactcttc aataaatggc gggatatttg
      781 cgaacgttcc cactgctcct gagatttttc ctgcttctac tccttttgcc gcatgttcaa
      841 atcgttcgat gttccgtttc atttctgaat accatgtggc aagtttcaat ccaaaagtag
      901 ttggttcagc atggacaccg tgtgtccgac ccatcatcac tgtatatttg tgttcttttg
      961 ctttttcgcc aatgatttcc gtaaaacgtt tcaagtcttc ccgtaaaata tcatttgctt
     1021 gcttgatgag atagccataa gcagtatcta cgacatccgt acttgttaga ccataatgga
     1081 cccattttct ttcttctccc aatgtttcag aaaccgctcg tgtaaacgca acgacatcat
     1141 ggcgtgttaa tttttcgatt tccaagatcc gatcgatatc aaatgatgca tgctcacgaa
     1201 tttttttcac atcttcttgc gggatttcac caagttctgc ccaagcttca tctgctaaaa
     1261 tctcgacttc gagccacgca cggtatttgt tctcttccgt ccagatattc cccatttcag
     1321 ggcgtgtata acgttctaac atgttttttc tcctctaatc ccaaatattc gtttgtttga
     1381 tttcttccag cgtttgctgg atgtcttctg tcaagatagt gatatgtccc atcttgcgac
     1441 ctttttttgc ttgttttttt ccatagaaat ggaattgcca gtctggctta ttctcaatca
     1501 agcgatagct ttccagcagt tgttctccca aaacattgac cattactgca ttgcttatca
     1561 aacgtacttt ttctcctaat ggccaaccac aaatacctcg aatatgggca tcgaactgac
     1621 tcatagaaca agcttcaata gaataatgtc cagaattgtg aggacgagga gctaattcat
     1681 tcacatatag cccgcctgtt tttgttaaaa acatttctac ccctaaaaca cctgaaaggc
     1741 tcactgcttc tgcaatcaca cgggcaatac gctcagcctc ctcgatcaca tcctgatcaa
     1801 tcgcggctgg agcaatcgtt tcatgaagaa tattgttgtg gtggatgttt tccacgaccg
     1861 gaaaagtcgt gaaatctcct tgaccatttc ctgccaccat gatcgaaatc tctttttcaa
     1921 acgggatcca tgcttctaaa acacaggttc cttctcttaa aaggtccatg gcaggagcta
     1981 gatccgcccg acttttcaaa acatactgcc ccttgccatc atagccgcct cgagtggttt
     2041 tcaaaacaca aggatagccg atcccgtcaa tcgcatcttg gatatccgtc ggactgacga
     2101 tcgttgcata aggagcaatc acgatgttat tcgtttccaa aaaagatttt tctaacaggc
     2161 ggtcttgcgt aatcgcaagc aagtctgtcc cttgaggaat aaatgacatg ggtaaaatcg
     2221 catttaacgc ctctacactg acgttttcga attcataggt aatgacatct gttcttctgg
     2281 caagctcttt cagcgcaaag gtgtcatcat agacactttc aatgtgccaa tctgcaactt
     2341 gtgcggctgg acaatcgctc acaggatcca gcacacctac tttaaagccc atttcttttg
     2401 cactaatcgc catcatacga cctaattgac cgccaccgac gatcccgatc gttgaaccag
     2461 gcaacaaagg tttaaccaag ttgatcacta ctttctataa cagttttagc caacattgaa
     2521 cgtctttcag ccaatttagc ctccaattct aaatcataca tggaaagcat ctgtatcgca
     2581 agtagtccag cattaattgc tcccgctttt ccgattgctg tagtagctac aggtactcct
     2641 ccaggcattt ggacaatcga aagaagcgaa tccaaaccat ttaatgttcg tgattggacc
     2701 ggtacaccga taaccggcaa agttgttttt gatgcgacca tacccggcag atgcgcggct
     2761 ccccctgctc ctgcaataat gattttgatt cctttttttc gagcgttttg tgcatattca
     2821 aacatcaaat ctggggttcg atgagcagaa acgacctttt tttcataaga cacaccgaat
     2881 tcatccaata tttgacatgc ttctttcatt gtttcccaat ctgaagtact ccccataatc
     2941 acagagattt ttgctgtcat tgtttttcct cccagttttt ctacattcat attttaacaa
     3001 tcactttttt tgatgtcaaa gaaatttcga atgttaacaa agaaaaacat tttattgttc
     3061 gtgtttatta aagataaaaa aaaggaatgt gacaaaagtt aggtcacatt cctttttatg
     3121 gataaacggt gttctcctgc caactcctcg gtcacaagga acaatattca acaaacatga
     3181 gaagcttttt tttgaaattg ttccttgtga ctcggagatg aacggcaggc tcacgacctc
     3241 ttccttcaac ggtgttcaat cagctgaccc tcggtattag ctcaaacaaa cggcaaaata
     3301 cgaggaacta tttttccgtt tgtttctcta acaatcaggt caaaacgctg atttcacaac
     3361 ctttcttttg ctctattact tcttgaaggc ttcttccaat atttcttcat cttttttcaa
     3421 tccattaaag atcaaattca atatgatagc cgtaatgcta ctcattacga tgccatttcc
     3481 agtgaacatt tgcaatgttt gcggcatttg actgaataat gtcggcgtaa cattgaaacc
     3541 cagcccaaat ccaacagaaa cagcaacaat caatagattt ttatcattgc tgaaatcaac
     3601 ttccaacagc atacggattc cttggactgc caccatcccg aacatgacta acattcctcc
     3661 accaagtacc ggctcaggga tgatttgtgc taacgcaccg attttaggga ataatcctaa
     3721 aacaatcaag aagaatgcag agaaatagat gggttttctt gttttgattc ccgaaagttg
     3781 aaccaatccg acattttgag aaaagccagt ataagggaat gtattgaaaa taccgcctaa
     3841 gataacggca agtccttctg cacgatatcc ctttttcaat tcttcttctc caacattttt
     3901 cccagtaatg tcaccaagtg caaaatatac acctgttgat tctaccatgc tgaccatgga
     3961 aatgatgatc attagtaaaa tcgaccatac gtcgaaagtt ggtttaccaa aataaaaggg
     4021 ttgtggaaca tggaacaacg gcgcatctcc aatcgcgctc agatcaatca ttcccatgaa
     4081 agcagctaag atgcttcctc cgactaaacc aatcaatacc gcgattgaac gaatgaaccc
     4141 ttttgccagc atttgcacgc ctacaattag gagaatcgtc acaaatgcaa gtagcagatt
     4201 tgttttgtca ccaaaatcag ctgctgttgc attacctcca cccattttct cgattgctac
     4261 aggaatcaaa gtcaaaccaa taacggtgat aaccgttcct gttactatag gagggaatag
     4321 ttttttaatt ttagaaaaga caccagaaac taacacaaca aagatcccag aagcgataat
     4381 agatccgtag atagctccta ctccttcatt ggttccgatc aagatcagtg gagctactgc
     4441 ttggatcgca catcctaaaa cgacaggcaa gccaattccg aaaaatcggt taactgtcaa
     4501 ttgcaataat gtcgctaatc cacacataaa gatatcaatt gaaatcaaat aagtcatctg
     4561 ttcttgatta aagcctagtc ctgtaccgat caaaagaggc accgctactg ctcctgcata
     4621 catcgctagc agatgctgca gccctaaaac cgcagctttt ccattttgtg tttctttttc
     4681 cacgattatg cgtcctcctc aagaaactca acttgtccat tcgtcaagga agcaatacgc
     4741 gcaagagaaa cgactctcaa tcccatttct tcaagtaact gacgtccatc ttggaaagac
     4801 ttttcgatga cgatcccgat tccttctact tgcgctccag cttgctggca taattcgatc
     4861 aatccttttg ccgcttgtcc gtttgccaag aaatcgtcga taatcaatac tttgtcttct
     4921 gaagataaaa atttacgaga aatagaaatc gtacttgtca cttgtttcgt aaaagaataa
     4981 acagaagctg tcaataattc ttcatccatc gtcaaacttt ttgcttttct agcgaagatc
     5041 attggaacat ctaattctct tgcagcaaat aaagctggtg cgatcccaga agcttcaatg
     5101 gtcacaactt tcgtgattcc ttgttcttta aatacttctg caaaccgttt tcccatttgt
     5161 tccatcaata cagggtctac ttggtgagtg acgaagctgt cgacttttaa tacgccctcg
     5221 cctaatatac gtccgtcttt ttgaatacgt tcaactaatt ctttcacttt atttgctcct
     5281 ttatcattta ttaataccga aataaccgct tttgtgcaaa aacctactat gtttctgtta
     5341 aagaaaaaca ccctccactt tatcataaga aaagaagggt gaaatagcca atcttttact
     5401 tatagtccag catttacggt actggctaga aactgtcatc catattatga cgatatataa
     5461 gtaggtatgt aaaactaata atagtgtaaa ttcattttct tgcaactgtt ttgcacgtga
     5521 aggcagtttg ccatatttga ataaaaaatg ttgtttcaaa tattgaaaac agtctaacac
     5581 tactttctga aactgacaac gtggtttact tatttttttg taaaatctaa tgatttttaa
     5641 tgcattattc ctttttgttc gggtttaaat agttattttt attttctcct atctaaaaaa
     5701 gcagcccaca taaaaagaag gcatgacaaa aagcatgtca tagccccttt ttccgaataa
     5761 atagtgttca aaagacagcc tatcggcaat aaggcaagat tttataaact acgggaagta
     5821 atttccgtaa atctattctt attcttttaa gctgacaagc tcgtcaatca ctactgcttt
     5881 tcctgaagca atagaatgat ttgctgcgat tcctgtcaaa atcgacaaaa taccatctac
     5941 gtgagttgct gcccgattcc acggatcttc ttctttttct tcaccaaata gatcatctaa
     6001 taaacgcgga tctcctccgc catgtccgcc cgcttcttgt tcgacgatta cctcatatgg
     6061 ctcaccgaac atcggataga catggatgga ctgaagaacg gctgctcctt catccttttt
     6121 atctccacct gcattgatgt aagacttttc gatcactttc atttcgatcc gtcctttgct
     6181 gccattgaaa gaaacattga atccttccca cggcatatac gcgtttagcg aatacgtcaa
     6241 aatcgcacca gtttcatatt tgaccgttac gcctagtgta tcttcaatag agatatcatc
     6301 ggaaaataca ctgcggtcac gaatatatcc actgtctttt tctgcttcta aatacatttc
     6361 tcgcaattcc tcattggctg acaggtctat agcaaatgga tcagctttag ctagttcgct
     6421 tccatagcat cgactgtaaa atatttctac tccgcgtttc tccgcattat gcatcccata
     6481 aaagtttaat ccaccgaatg caaatacctt ttggggacgg ctgttcaacc aaaaattcac
     6541 tagatcaaaa tgatgggtag atttatgaac aagaagcccg ccgctattgt gcttatttcg
     6601 atgccatctt ctgaaataat ctgccccgtg ttgcgtattt aacagccatt cgaagtggat
     6661 agaaaacaca tcaccaatca catcactttc tatcagttcc tttatcttcg tgtgatacgg
     6721 tgcataacga taattaaaag tgactcgcag gcttcttccc gtctgttttt ggaccgttat
     6781 tatttcttga gctttttcct catcgatagt cagaggtttt tcgcaaataa catcatatcc
     6841 ttttttcatt gcacgaatga tataatcatg atgcgttcga tctactgacg taataatgac
     6901 ttcagccggc cgtacgtcat ccagtagttg ctcaaattgt gatggcatac atgtctttac
     6961 ttttttctgt ccatacttgt tcacaagtat ccgatttgca taatccatcc ttgtttgaga
     7021 tacatcacaa aaaccgacta gttcacaagt ttgatgatac gtagaggcaa ttgcctcata
     7081 aaaaaaacgc gctctcccac caagtccgac ttgtacatat tttttcttca tttaaagtca
     7141 ttctcctttt cttttaagca aaatactgtg acaaatggca gccctggata cagtaatgca
     7201 gtgagtaaaa aatcatgctg tatacaccag aacagtccat acaaaaggca taaataccac
     7261 agaaacccct tgacgttttt tgacaacagc cagatattcc ttttgatcaa ataaacaaga
     7321 ctatctgctt tatcttcttt gcagatatca attagcgtac taaaaaaact atgggataca
     7381 aaaagcaaca gacacaatag aaaaggaatc agataccaag ctccaatctt tcccactaat
     7441 aaataccaat caactgcaaa aataatgaca aacagctgaa acaacgtaat gatgaaacac
     7501 atccgtttga tttttccttt cctccaacat accttttttt ctgccaagta atggacagat
     7561 agataataag aaggatacat catcaaaaaa taaatcagat agacaacacg agcagacagc
     7621 actaatccta aagtgaacaa acatcctaga actaaagcga aaagagcatt actgataaga
     7681 ctggttaaaa aaatccgaca gacatagtag ttgagcttgt aaagaaacat ctcttctatt
     7741 ggctttttca tattcagccc ttcagaccgc ttgtactgat cccctggaca agatatttat
     7801 tgaagaataa gaaaatcaga aaaaccggta gcagcgacaa cgtagacatc gcaaacatac
     7861 tcgcgtaagt cgtactagat ccagcgtctg caaataattt gatggcaatc gataccgtgt
     7921 aagatctcgg cttgtttaaa taaatcaatg gtcccatgaa gtcgtcccat ttccaataaa
     7981 actggatgat gatcgtcgtg atgatcgaag gtttcagcaa aggaaaaata attttggtaa
     8041 agacactata cttgctgcat ccgtcaataa tcgctgattc atctaactct ttggggatgc
     8101 tgaccatgaa ttgcatgatc tggtaaatga aaaatgcttg tccaaaaaaa tgtggtaaaa
     8161 tcagcggcac atacgtccca accaaattca gccgattgta aataataaac tgcggaatca
     8221 aaatcacttg tgcaggaatc atcatcgtta ctaacatgac gacgaaccag aatctttttc
     8281 ctttgaaatc gattctcgat aatgcgtaag caatacaagt cgagctcaga actgtcccta
     8341 tcgtcgaaac tgcagagatg acaaacgaat tcctaaagaa agtaaaaaat gaaattccag
     8401 caaaccccgc ccatcctttt gcgtaatttt cccattgaaa agtctctgga atcaaattca
     8461 gcgaaccact cataatctct tgatccgttt taaaagaacc tgcgatcatc cataagaccg
     8521 gataaatcat gactagacct aatagcaaaa taaaaccgta ttgaaaccat ttcgttactg
     8581 tattattcat ttcttcctcc tctcattcac acaacgaccc atttttttga tgatttgaaa
     8641 atcagaaagg tgacagctcc cataatcaac agcatgatcc acgaaatagc actagcatac
     8701 cccatagaat taaaagaaaa agcttggtta tacacataaa gagcataaaa gttcgtggca
     8761 tcattcggac caccttgggt aatgataaat gcctgcgtaa aagtcataaa cgccgcgatt
     8821 gtctgcataa ccaagttata caaaatgatc ggcgaaagaa gaggtaacgt gatcttgtaa
     8881 aacgtctgcc atttgttcgc tccatcgatc tctgccgctt cataatacga gcctgggatc
     8941 tctttgatac ctgcagcaaa gatgatcatt gccgaaccga attgccaagc agacatcaaa
     9001 acaagaggga tcatgaccag tttttgatcg ctaaaccagg aaagcgcagg tgcgcctaat
     9061 cgttccagga aaatattcac taacccgttt ttagaaaaca actcccgcca aactagagcg
     9121 atagaaatac tgccaccgat caaactaggc acataataca aagaacgaaa gaaagtcgtt
     9181 cctttgatat tcctcgttaa aagataagca atcaatagac caaagatcag tttcacaggt
     9241 acagatgcaa atacatagag aaaagtgact cggatggagt tcaaaaaacg ggcatcactc
     9301 agcatcttta tataattatc caagccgatc catgagactg gatttcccaa acgataatcg
     9361 gtgaacgaat aataaagtga caagatcatc ggtatcactg ttatcaatag aaatcctagg
     9421 atgaatggag aagcaaatac gtaaccgacc acattgtctt gatccaagaa ccgttttagt
     9481 ttttcttttt tcatgccttc ctcctgctgc tatcctagaa tgtttgacgc aaactcataa
     9541 agactctctg ctgcttcttc tgctgttact tcttcataaa tgactttttc aactaaaaga
     9601 ttgtattggt cttgaatttc cggattcaaa gcactatcaa agacattcac ttcgccgttg
     9661 tccatatcgg aaatcatttc taaataatca aatgtcgctt gcaaagttgc atcatctgca
     9721 cgagcgagct gttctcgaat attcgacatg attggtacac cacgttctcc ttttaaaatt
     9781 tcattggctt cttcactgtt ttgaaagaaa tcaataaact tagcagcttc ttctttatgg
     9841 gctgagcttt ttgccatact cagcatttgc gaagaacgta aagggatacc agattctcca
     9901 tctgcttttc tgcgaggtgg attaatcaac tgaatctcca tatcttcagg agcccctgac
     9961 aatatttctg tcaactggtt acttgataga aacaccattg ccgcttctct atccctaacc
    10021 gcctgaaaag acagatccgt cgtacttgca gctccaccac ctggttctgg atgggcccct
    10081 ttgtctacca gatttttacg cagctggaaa aaatctgtta gatagtctgg attttcaaat
    10141 cccaagctgt tagatagatc ttcccgatta taaaaattca atcctttctc aacttgtggt
    10201 atttgaataa tacagccagc tataaaatct tcgaatcttg aaatagcata gattcctaat
    10261 tcttcagtga tttgatagct gatttgttca aattcatccc atgtccaatt tgaagctggt
    10321 tcagggatat ttaattgttc aaatatattt ttattgtaag caatcgcaaa cgaattcacc
    10381 ccgttagaca gtccaactaa ttgcccgtcg taagtcgtcg ttgctaaata ggattcatct
    10441 gcattagaaa tatcaattat cccttgctcc acatattcat ctaatggttc aatctgattg
    10501 atgtattgag gaaattgatt gttcagttgg aaaatgtccc agatatcatc tgatgctgct
    10561 agtgtaccta attttgtgta atatccttca taatcataaa attcataatc gatcgtcaca
    10621 tggggatttt tttcttcata aagctcgatt acttttactg ttgcatcgtg tcggacttgc
    10681 gaaccccacc aagccatgtt cagcgtgatt tttttgtcat cttcttgccc acaagccgtc
    10741 aagaagaata acataacact taaagctaag atgattctca ttcttttctt cattctctgc
    10801 acctctactt ccattgttat ttttcaatat actgtatttt tatacagaac atatttatta
    10861 aacgatcttt ttcaagggga ataaaaacca agacttgcct tatcgttctg cctaggatga
    10921 acaactcaat actttcaaga aaaattagat agtgctgatt cttctttcat cgctaaaaac
    10981 acttcttgct tcaatgatgg gaggataatt acgcaaagaa aaaaggatat cagacaactg
    11041 cccaatatcc ttgacatagc taaataagca tgcttcttcg tgtgcgctct tccctaaaaa
    11101 acagaaaaat aactagtaat tctgtatgaa attaggtaca atatacacgt cgatgcacaa
    11161 gcattgacta tggcaacttg attggtcagt tcccgctgac ttttcaagcc ccgacaattt
    11221 agctgctact aaattgtcgg gtgccttttt tagtttttat tcgtttttca ttctatagaa
    11281 agcgcttcaa taaatcaaga catttttttg ttagaatgct aataataaca aaaggtttta
    11341 actcttctta tgtacattac aggtaaaaaa acaaaaaaat ttctcttttt tttgaattct
    11401 gaacaaatag tactggagta caaaagacga ggaagagcca ctatctgctc ttcctcgtct
    11461 tttgattctc acctgttatt catgattctt tttctcagtt tcccgatatt tttccatttt
    11521 acgactaaaa agctctccgc tacttggtgg cgtatatgtg atggcatttg ctcctgcttg
    11581 aatggtctca gcaatacttt cttccgttgg accacctgtc gcaatgatag gaagttcagg
    11641 atatttttta cgaaaatggc gtactgcttc tgcagtttct ttacctgcac tgatattgat
    11701 aatcgaaata ccagctgcta acttttcatc tatttctgta tatttcgacg ttaccgtact
    11761 gataactgga atatcaacta ctcgacaaac ttcttttact gtctctaaag gcgtaggacc
    11821 gttcaaaacg acccctaacg aaccttgcgc ttctgcgaac atgctcatgt aggaagaacg
    11881 agcaccttgt gtcaaaccac cacctacacc ggagaaaact ggaatgtcag ctgcctcaat
    11941 aatgctttta gtaatcgctg gatgaggtgt aaaaggatac acagcaatta ctgcattcgc
    12001 atttgtatta cgtatgatcg caatatccgt agtaaagatg atcgaacgga tcaattttcc
    12061 aaatatcttg atcccgcttg cttctaaaat gatttccggt actcttacga tatcttttct
    12121 caattccgtc atgatttctg gcgtaaattg ttctgccatc agccacttct cctttgctat
    12181 ctccggtcat actgattcaa ttgatacgtc tcgatgacct ggatgatttt ttgcgcataa
    12241 ccggggtctg tcgcataacc tgcatcttgc aatgcttgtg cggcttcctg atagttggtg
    12301 gccgtaatca ctttttcata cagcgcagga ttccaatcta ctccttggac gaacagttgt
    12361 gtatggtcat ccatggactg ttcccatgaa tcatacactt tgaagtctcc ttggatagtt
    12421 atccattctt cgttaacaaa ttcctttgtt tccaggctta ctttcttctg atccccataa
    12481 gccttgatcc cgaaaagatt attgtatttg ctagccaagg tactttttcc ccaatttgat
    12541 tctaatatag cttgtcctaa aatgatacta ggaagcactc cataaccatc ttgtagttct
    12601 tgggcatgcg gaacaagccg ttcaataaat tgctgatggg attgcgcttc attttcttgc
    12661 tgattatcag taaaaggaga agacagattc ttcaaggaaa aaacaaaggc taagagtatc
    12721 actgctacac cggcaaccac tgctggccaa cggatattct tgcgcttttg ctttttccac
    12781 ttttttttcg ccatgatcaa ttcctacttt tcatttaatt cattcagata ttcttctagc
    12841 gtcaattggt gtttggtgat atactcagcg ttttctttac cgacataacg taagtgccaa
    12901 ggttcatatg tgatcttagt tatatcttcg tgtccttttg gataacgaac gataaatcca
    12961 tactgcgcag cattatctgc gatccattta gctccaggct gatctccgta acttgcatcc
    13021 aatacagtag aaggataact gttgtaccag ttttgatcga cgacatcgac tgccagacct
    13081 gtgtggtgtt cgctgtatcc tggctccgta atcgtttctt tagtaatctt tgtcgcctcc
    13141 tcttctgata caccttgacg actcatcaca tcttggacat tttgttcgaa aacttgttgt
    13201 tgggatgata cagaccggta agctgagacc atcactaatg gaaatccagc tgcttcagca
    13261 gccttggcaa atgcttcata ttctggagca atgcgtttgt cgatcaagta tccgttactt
    13321 aatgatgcca attgagtaga ctcatcaatt tctttttcta atttgtgatc tggaccaacc
    13381 aaaactagct tccaatcgtc tgttttaaca tctggcaatt gtttggacgt acgatctttt
    13441 gtttctttgt cctttacctt gctgctggtt gtttgactgt tcttttcttt actagcagtt
    13501 gttttggtgc ttgttcgttc gactgcttga ttgccggctt gaacatgcga atcatcatcg
    13561 ctaaaaataa catagcccat tgctgcgatc ataatcacaa ctgctccaaa tcctaaaaat
    13621 ttattcactt gaaaaatcct ttcgttgttc ttcacgttcc ccattcgcct catgaacgta
    13681 agtttcttcc agattttcat tcacccaatc gagaagttcg attttttctc cttcgatctg
    13741 tttttgaaca tctgctggca gacgaaaatt caaaatatca tcatagcccc attcatcata
    13801 ttgaatactt actttcaagt caaggtatct accagcctgc tgattatttt tcaaagggat
    13861 cagctcgact tttgctgctt tgctgctaag ccatttttgc gcgatgcgtg ttttgatttg
    13921 cttgtactct gcaacggcaa cgtttcgttt ttcgggaaaa ataatcgttt gacgcaaaat
    13981 cttttgcgtt agcatccatt cataatcagt gaaaagattt tttactttct gcatcgcttc
    14041 agatggcaga tttttctcgc catctttcca tttttgccat tgttctggac tgatcagcaa
    14101 gtattcatct aaaaaatttt tttcagtttc aaactgttct aataatacac tcataataag
    14161 atcaatatat acttgatgca cgtttatccc ctccttttaa ctattctact taagttttga
    14221 tcaaaaagca cctcttaaat cgctgctttc atttttttta atgtttcaag tcctctcctc
    14281 tttattcttc gtgatttttg ttataataat agacagacaa gaaactcaat ccaactatga
    14341 aggagcgttt tcagatgaaa aaatatcaat ggggaatcgt tggtcttggt accatcgcac
    14401 atgagtttgc cgaaagcttc aatcaagaga cgagcgaact aacagcagtt gcttccagaa
    14461 cttcagaaaa agcggaaaac ttcgctcacc gttacaatat tccaaaagct tacggaagct
    14521 atcaagaaat gttagacgat gctgaaatcg acatcgtgta cattgctgta ccaaatagac
    14581 agcatatcga ccatatcttg gcagcattgg aagcaggcaa acacgttcta tgtgaaaaag
    14641 ccatcacgat gaataaaaaa gaactggctg acgctatgcg cttagcagaa gaaaagaatt
    14701 tgatcttagc agaagcgatg acaatcttca acatgccact ttatcagcag ttacgctcaa
    14761 taatggatac aggaaaactg ggggctttaa aaatgatcca agcacctttc ggcagttaca
    14821 aagaaccaga ccctaaaaac cggttcttca atccagaatt agctggagga gcactgttgg
    14881 atatcggtac gtatgctgta tctttcgctc gtttcttttt gagctctcaa cctgaagtag
    14941 ttgcctctac aatggttcct tttgaaacag gcgtggacga acaatcagtc actatcttgc
    15001 gtaacaaaga aaatgaactt gctgctgtca gcttgacttt tcaggcaaaa atgccaaaag
    15061 tcggtatcgt tgcctttgag aacggttatg tgacgattgc tgattacccg cgtgctgatc
    15121 gtgcggagat cttatttaca gatggtacaa aggaattcat cgaaagcggg aatacatctg
    15181 aagcaatgaa ttacgaaata aagaatatga tacacatgat tgaaggtgac ttgcccaatc
    15241 gctctctctt cttgacaaaa gatgtcatcg aaatcttgga tcaaatgcaa gttctatgga
    15301 aaaatatgta attttaaaag gttgtgaaaa agctgttctg catcaagtaa taagaacagt
    15361 caaacaaaaa tagctcctcc tatttttcgt ttggctggac ttattaccgc agatgcaagc
    15421 ttttgaacac cgtttaagga agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    15481 gcaatttcaa aaaacagctt ctcatgtttg ttgaatattg tgacttgtga ccgaggagtt
    15541 gacaggagaa caccgtttaa agaagaggtt gtgaactcag ctttttgacc tgaatattag
    15601 ctgaattcac aacctctatt tttggatttt ttgttgggag aattctgcct tgatttcaat
    15661 aaaactttta aaagactgca aaaactggaa caattctgct cgatcctcga attcagggcg
    15721 actagtcggc agaggcattt gccgatacaa tacataaact tcttcaatac gtgctaaaat
    15781 ttgtctgcca tcatttgact ctgaaaatgt ttctgcagta taaatcaata aaccatagat
    15841 atgttttcca taataagctg gttgctggat tctctctaga ttttccagca tgtctttaat
    15901 cacattggcc tgtgctcttc tcatagagaa gcaagtttcg tagtatagcg gctgtctgag
    15961 ccagtagttt tcttgatgtt ctctggcatc ttcttggctt tcgcgaatga atgtcagtaa
    16021 acgttcgcat ttgatctgca catcttcttt attttctgat aaaagaaatt ctgccatttg
    16081 tctcaaaata tttcgaaatt ctttttcgat ctgtatttgg ttttccttca gctgcttcgt
    16141 acgatctggc atgtaaacat ttgcaagcaa agcaaatact aatccaatcg ccattaatcc
    16201 cgcttcattg ccgatcagct gccaagacat gttttcctcc actaaataat gagtgaccaa
    16261 aaccgaattt actacgatcc cttcggttaa tttgaagcga gaggcaagcg ggatgaacag
    16321 gaataaaaaa agactgaagc caaaaatagt atacccaaca gcaaaaaaac tgaacagtgc
    16381 tgttgccgta gctaatataa aggctgcgat tcgattcatg cccgtacgca atgtagagcg
    16441 cttggtattt cctacactta ataatgcaat gatccctgca gctggccaat acaacagcga
    16501 taagctaccc gctgtgacca tcgaaaagaa gacactgatc actgttttga ctgttcgcat
    16561 tcctatacgc atttacttcc cccttttttt attttaatta tacttgaata atgaaaaact
    16621 agctatcaga caaaagttta ggtatgatag taaaaagacc aaaaaggaga atctctatgc
    16681 aatttggccg ttttcgacta ggtatgcgta cgatcaaaag tgccatagct gtttttttat
    16741 gtatcttgtt ttttcatttg actcaacgag gaatcccgat gattgctgca ttatctgctg
    16801 ttttttcttt gcgtcaggac ttgactacaa ccgtttcttt tggaaagtct aggatcatcg
    16861 gcaatacatt aggtggtaca ttagctatca tttatttttt tgtcaaaagc tattttcaca
    16921 atgatttttt ggtagaattg atcttgcttc ctattctagt tattattgtg atcgtcgtgt
    16981 cagatggtat taacaataat tccggtatca tttccgcaat cgctacgttg ttgctgattg
    17041 cgttgagcat ccctcaaggc gaatccgctt tgtatgctat ccaacgtgta ctagatactt
    17101 ttatagggac attcattgca atcggtatca atttctttct tcgtcctcct gaaacagaaa
    17161 agaaggaaga actatcagaa gatttggtcg aactccaaaa gaaagaacga ttgctaagaa
    17221 caaatttaca agagatagaa gctaagatcc aaaaacaaaa gaaaaatagc gaggactaat
    17281 tttcttttgc aataaaataa aaaaatggag agaagaactt taaaaagtct tcctccattt
    17341 ttgattgaac ggtgaagtgt actattttat tcttcattca tcgagttttg ttctgcctct
    17401 tttggcgctt catacatatt tacttggcta gtagtcccac gtgtcgacaa attctctacg
    17461 atttccttca agtcaagtcc tgttgtcgct tttagtgttt cttgagtaga agaaagcaaa
    17521 tttgtggcat aatttgtcac tcggttcgct ccactggttt ctcctgaact gcttcctgta
    17581 tcgacaacag agattttttc gatatttcct agcggctgtg ctgcttcgcg catcaattgc
    17641 ggtagcatat cgataaccat actcaagaca gctgcttcgc catattcctt gaaggcattg
    17701 gcgatctttt gttttgcttc ggcttcggct tttccttttg ctagtgctgc ttcggcttct
    17761 gcttgtccag ctaaacgtgt cttattggct tccgcttctg ccagtgcttc cacacggaat
    17821 cgttctgctt cggcttcagt cacttctttg actttttgag cttgtgcttc ttgttcttta
    17881 gcgtaccgat cggcatctgc ttttttcttc acttccgaat catattgctt ttcacgacgt
    17941 gtgatttctt tttcttccag ttcgatttgt ttttgacgtt ctacgatttt aatctgcatt
    18001 tcctgttcga ttacttgttg ttgtgctcgg gcactttcta aattgtaggc ttggtctgct
    18061 tttgctttag caacatcttg ttcttgtttg taggttgcta atttcagttc tttttctttt
    18121 aatgattctg cgatctctgt ttgtcgctgt aattcagctg cttgtgattc tttttctgct
    18181 tgagcttttt tgatacgcgt ttcttttaga gcttccgctt ctgctatatc tgcatctctt
    18241 ttgacttggg cgattcttgg tttccctaaa gagtcaagat aaccgttttt gtctctgacc
    18301 tctttgatag tgaaagaaac gatgatcaac cccatttttg caaggtctac agaagcgact
    18361 tcttgtacgc tttgactgaa cttatcgcga ttttgataga tttcttctac agtcatagaa
    18421 ccaagaatcg agcgcaagtg tccttctaat acttctcttg cttcattttc cagttcttct
    18481 cttgtcttgc ctaaaaactg ttctgctgct gtagcaatct cttctacaga tgaaccgatt
    18541 ttgatgatcg aagtaccatc acacatcacc ggtacgcctt gttccgtata cacttcaggg
    18601 gtggagacgt ccaatttact cgataataga ctgatccgat tcgaacgttg aaaaactggc
    18661 aagacgaatg cgccacctcc acgcacaatt ttgattttgt tattgctttc atcaatgtgc
    18721 acatttttgc ttcctaaata acttccactg atgatcaacg cttcatccgg ttttgctgtc
    18781 tggtacttcg ctacaaagac gatcagcaac atcagtaaaa tcaaagctgc gattgcgatc
    18841 gcgataaaaa caactggtat ttccatttaa cacacgctcc tttaagcttt tatcatgctt
    18901 tcataaggca tgacataaca gattctttcc ttgatctcaa tgatcaatac tttcgttccc
    18961 cgctcagctg tctgttcttg ggggctgtaa aaacaagctg gacgactaat ggaaccggta
    19021 acacttttca actgaatttc tcccatacct cgtacaggga tcggtgtgat cacaacagct
    19081 acttgtcctt ccagagtttt ttcagaagac gacatcgttg attcggcatg tttcatcggc
    19141 atcaatatat agaaattaag caaaaatacc aacactatcg atatgcctcc actagcaata
    19201 aataaaaata aagatgattg atctgttaaa cgctcaccta gaaaaccaaa caagcttgtg
    19261 aaagttaacc aagggataat aagcatcgga tcaagcgggc catcaaaatc gaatacatct
    19321 ccaaaaataa tcaataggac tgccaatcct gcacaaatca gcaatgtata ccaataaatc
    19381 gtctcaagtg cgattccccc tatcatatac acgcctcctt ccatttctac gtctatcata
    19441 ccaatgtttg aaaattctgt atagcttctt ttaccgactc agtggtgttt tttataggaa
    19501 ggttgtgttt atcgttgaca atttgttaaa tttttgtttt catgttaaaa ttttcacaat
    19561 aaattctttc gaggtagcct atgtttcctt tttttaagaa aaaaccatta aagctccaga
    19621 acacgatcat ccttgtcgtt tgtttctcca cattcgtttc tttgattgtt tctgccatat
    19681 tgatcaggaa ttttattctt tcgatgaacc gatctagtgt gtcagataaa atctctagca
    19741 ttgctaaaat cgttgccaat gataaactca tccaaaaaaa gctgcagact ggtgacccaa
    19801 cacatgcgat tcaaggaata acaaatgaaa tcatgattgc atcgaattta gattttatcg
    19861 tggtcatgga ccctgaaggc attcgatatt ctcacgcaga cccaagttac atcggtcttc
    19921 ctttctcaag tcatgaagat gtgaaaaaag tgattggagg acaagaattt ttctctgaaa
    19981 aagaaggaat gttagggttc ggcttccgct atttcgttcc tatttattac aatcaagaga
    20041 ttattggtgc agtttgtgtt ggcttaacga acaacacgat tgacaatcaa gtcaaagatg
    20101 gacaaaaaat gattgttttt tcattgatta tcggacttag cgtaggcatc ataggcgccg
    20161 tttttcttgc ccaaaaaatg aaaaaagtac tgcttggact tgaaccacaa gaaatagcag
    20221 aacagttaag tgaaaaaacg attatcgaaa atgaagtaga tgaaggaatc ttagccatct
    20281 cttctgataa aactatcctc ttgatcaata cacctgcaaa aaacattttc taccaaacag
    20341 agcagctatt ggatttcgaa gtaggaaaaa aaatcagtga taatctatat gacgttttgt
    20401 ttaaagagac gattgagcat caaacgaccg ttcggactca atccatgtat ttaaatggga
    20461 tagaaactat ttctagcgtt acacctcttt ttagcaatga ccgtttttta ggagcagtcg
    20521 caactttacg aaatcgatca gaagtaatga aattaatgga ggagcttggg gggacgaaac
    20581 aatatataaa ctccatgaga gctcaaaccc atgaattcat gaataaactt catgtcattt
    20641 ctggtttgat tgatttgaag aagtatgaag aagtcaccca ttttatccag cagctaaatc
    20701 aaaattataa aaaagagatt ggccaagtct caagtctgat caaagttcct gcaattgccg
    20761 gttttttatt aggcaaaata aacgaagcca aagaacaatc tgttgaaatg ctgttagaaa
    20821 ccgattcttt tatccctaaa atggagatgg atgactccat ccattcactt ttgcaagtgt
    20881 tagggaactt acttgataat gcaaaagaag ccgtgctaca aaaacatgaa agaaacggac
    20941 aggtccggat tcatttaaac tatgacacag aagctgaagt attcatcgtg accgtctgcg
    21001 atgacggtgt cgggatatca gaagaaatct cagaaaaaat atttacgcta ggtttttcga
    21061 cgaagggaat taatcgtggg tttgggttga atctcatcca ttcaatcgtc aaacgccacg
    21121 gtggattttt agaattttcc aaacaaacaa atggcggtac aatcgtctat ctagaattac
    21181 caaaaaagga agatcaaaat gatgacaaat gtcctgataa tcgaagatga tccaatggtt
    21241 gcagaattga atcgtacttt tgtggaaagc atccctaata tgaacgtaat cgaaagtttc
    21301 agaaatgtag aagaaggaaa aaaatatttc agacaaaaaa acgcagatct gctgctgctt
    21361 gatgtataca tgcctggcga aagtggtatc cattttttat cttggcttag acagcaagga
    21421 aatgatatcc cagtgatttt gatcactgct gcagatgata tcgaaaccat aaagaatgtg
    21481 atccgctacg gtgccttaga ttatttgatc aaaccgttca ctttgcaacg ttttcagctg
    21541 gcgatcgaaa agtatcttgc tttctctcag acaatccaaa cgactgaaaa agccgatcag
    21601 aaattgatcg atcaagtatt caaccacacc actcccattc acgaaatacc atcattcatc
    21661 gatttgccta aaggtctgtt aaaactgact ttaaaaaaaa taagtgcatt tattctaaaa
    21721 gaagatacta ttttttcaac aaaagaactg gctcaaaaaa gctcactgtc acgtatatca
    21781 gtcaagaaat atttgaattt tttggtagaa caagatatat tgaacgaaag aatcaaatat
    21841 ttggaaatcg gccggccgct aacctgttat gaattgacaa agaatgggcg gaaaaagctc
    21901 acagattact gttgagtcat cgaattatcc catacaaaat agtccaagta tacagaagta
    21961 acaactgttt acttggacta tttttaaaca ttttattgac gaatatccat gttttcttat
    22021 cttaattgac gggctttagg aagagatacc tcatttttcg tttttccagt ttctattatt
    22081 tcacgaaagt tctcgaaact tgtcgaaatc atatttgata atgcctcatc tgtatttgaa
    22141 cctacatgcg gggttacaag aactctaggg tacatttcta ctaattgctg gatagcaggg
    22201 tctggaatcg tctcccatgg cttgaaggat cggaaaaaaa cctctgtttc atttgcaaaa
    22261 acatcggttg caaaaccagc taaatgattg cttttcaaag ctcgtgaact actcaccact
    22321 tagctaaacc taacggtttc ttacg
//
LOCUS       DANKEF010000044        22073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000044 DANKEF010000000
VERSION     DANKEF010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.044"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..221)
                     /locus_tag="PWZ48_001908"
     CDS             complement(<1..221)
                     /locus_tag="PWZ48_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261561.1"
                     /translation="MLMEGKTQLWFKFDPSNRFIKDFYKVWNSEVFFLAIEDSLLINL
                     YYSNKNYFKIPAAKTRMKKGVYFLFDIVT"
     gene            complement(470..670)
                     /locus_tag="PWZ48_001909"
     CDS             complement(470..670)
                     /locus_tag="PWZ48_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2261562.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(975..2207)
                     /locus_tag="PWZ48_001910"
     CDS             complement(975..2207)
                     /locus_tag="PWZ48_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDL2261563.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            2461..3030
                     /locus_tag="PWZ48_001911"
     CDS             2461..3030
                     /locus_tag="PWZ48_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2261564.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(3208..3648)
                     /locus_tag="PWZ48_001912"
     CDS             complement(3208..3648)
                     /locus_tag="PWZ48_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDL2261565.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            3806..4570
                     /gene="map"
                     /locus_tag="PWZ48_001913"
     CDS             3806..4570
                     /gene="map"
                     /locus_tag="PWZ48_001913"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDL2261566.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            4602..5525
                     /locus_tag="PWZ48_001914"
     CDS             4602..5525
                     /locus_tag="PWZ48_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HDL2261567.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            5601..6740
                     /locus_tag="PWZ48_001915"
     CDS             5601..6740
                     /locus_tag="PWZ48_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HDL2261568.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            6733..7533
                     /locus_tag="PWZ48_001916"
     CDS             6733..7533
                     /locus_tag="PWZ48_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2261569.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            7533..8360
                     /locus_tag="PWZ48_001917"
     CDS             7533..8360
                     /locus_tag="PWZ48_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2261570.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            8338..9072
                     /locus_tag="PWZ48_001918"
     CDS             8338..9072
                     /locus_tag="PWZ48_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2261571.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            9172..10038
                     /gene="rfbA"
                     /locus_tag="PWZ48_001919"
     CDS             9172..10038
                     /gene="rfbA"
                     /locus_tag="PWZ48_001919"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDL2261572.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            10052..10624
                     /gene="rfbC"
                     /locus_tag="PWZ48_001920"
     CDS             10052..10624
                     /gene="rfbC"
                     /locus_tag="PWZ48_001920"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HDL2261573.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            10646..11674
                     /gene="rfbB"
                     /locus_tag="PWZ48_001921"
     CDS             10646..11674
                     /gene="rfbB"
                     /locus_tag="PWZ48_001921"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDL2261574.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            11772..12623
                     /gene="rfbD"
                     /locus_tag="PWZ48_001922"
     CDS             11772..12623
                     /gene="rfbD"
                     /locus_tag="PWZ48_001922"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HDL2261575.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            12658..14691
                     /locus_tag="PWZ48_001923"
     CDS             12658..14691
                     /locus_tag="PWZ48_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261576.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            14735..16015
                     /locus_tag="PWZ48_001924"
     CDS             14735..16015
                     /locus_tag="PWZ48_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HDL2261577.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVTEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            16225..17031
                     /locus_tag="PWZ48_001925"
     CDS             16225..17031
                     /locus_tag="PWZ48_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261578.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            17043..18263
                     /locus_tag="PWZ48_001926"
     CDS             17043..18263
                     /locus_tag="PWZ48_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2261579.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGKVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            18253..19839
                     /locus_tag="PWZ48_001927"
     CDS             18253..19839
                     /locus_tag="PWZ48_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2261580.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLYINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAQPQNSNYVKVLEVIQQTKQDEIIQQIPFNNFTGENETLSFHVSELA
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKDLPEFFLTDIAG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHYLIENNTRLD
                     QTLKTTINRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSLCIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVLREEVNQAKQLAEGAKAGFELRILCEK
                     ERSFLVEAVAQNGQSWIIEM"
     gene            19878..22016
                     /locus_tag="PWZ48_001928"
     CDS             19878..22016
                     /locus_tag="PWZ48_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2261581.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEVQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRQENGHISLATNSALKMAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDLIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPAEHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVHPGRISGFYEITYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYPNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTAMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     ILEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPSYIAHDPFYNDNLTKEGIGDFSLRPD"
BASE COUNT         6972 a         3737 c         4571 g         6793 t
ORIGIN      
        1 tcagtcacga tatcaaacaa aaagtataca cccttcttca ttctagtttt cgcagcagga
       61 attttgaaat agttcttatt agaatagtag agattgatta ataagctatc ttcgattgct
      121 aaaaagaaaa cttctgaatt ccatacttta taaaaatctt tgataaatct attcgaaggg
      181 tcaaatttaa accataattg tgtttttcct tccatcagca taaaagttca cctcaaaaag
      241 agtatacgaa cgaatgttct ttttgtgaag gcggaattta atatactgaa taatagaaaa
      301 caagtacttg tgccaaatta tgtgccaaaa aaattcgaat tcaacaaaat gtaaacaaat
      361 agaaaacctt ctatattaag atttcctgca ataacaaaca caacagaaga cgtgtaatag
      421 ttagtcagga acgtacaaat aacccctgta tcctttgcgg tacagggggt tattttgtat
      481 aagtagacat tgtagtttct aaactgctat gtcctaaacg tttcgatacg ctaagtatat
      541 ttactccttg ataaagtaaa atagatgcat gcgtatgtct tagtccatca ataaacagtc
      601 gaagagaaaa tagggaaaaa ttgattacta aagaaaaact tactaggtcg acggtgtagt
      661 tgattttcat acttcaatag attcgaatta aacgatttcg attggcttat accagtccta
      721 tccaacgaaa gatatatcgt tcttaactct agcggtataa cttgaagctt tacgtaaagc
      781 caatgggaat gaactggaga tgacttttta ttatttttag taatagctga tgggttaata
      841 cttacaactt tccttttcaa agtaccatat aaaaaaacgg tggggttgtt tagcgaaata
      901 aaggatctaa gccaatagct tagatccttt attctatgct ttatattatt ttttgcatgt
      961 gtgcgaaagt atactcaagc ccaatcgcca tttcggaaaa tcggtactgt agagccatct
     1021 tcacggattc cgtcgatatc catcttgtca gatcctacca tgaaatctac gtgggtttga
     1081 cttctattca atccagcttc tgctaattct tcatcggaca tttctgtacc gcctttcaca
     1141 ctgaaggcat aagcagaacc tagtgctaag tgattcgatg cgttttcatc aaataatgta
     1201 ttgaaaaaga tgatgccaga ttgagaaata ggtgaagggt caggtacaag tgcgacttct
     1261 cctaaacgac gcgcaccttc atctgtatca agcagttttg ctaagacatc ttctccttgt
     1321 tcggcagaaa aatcaacaac ttttccatct ttaaaagtaa atttcatacc cgagatgatg
     1381 gtgccagcat aactcagtgg ctttgtactt gaaatgtaac catctactcg atgactgtca
     1441 ggtgctgtga acacttcttc cgtcggcata tttgccatga atttttctcc acgagcatta
     1501 tagcttccag cgccttccca aagatggttt ttaggcaatc cgataatgat atctgtaccg
     1561 ggagcagtat agtgaagggc agaaaattgt tctcgattca attcttctgc tttttttgct
     1621 aatttttcat catgcttctt ccatgctaag acagggtctt cttcatagac acgagtcgtt
     1681 ttgaaaatct gatcccataa tgcctcaact tgtttttctt ctggcagttc tgggaagact
     1741 tttgctgccc attgtttacc agccgcagcc acgacagtcc aactgacttt atttgcttga
     1801 gttgccttgc gtaaattcat tagtgctttg ccattcgctg attgataaga cgctactcga
     1861 tggctatcta caccagcaaa tgcatcggga tcggcagaaa caacgctgat cctgcttgct
     1921 cctttttcaa gccattcatc cgcttgatcg attttagatt gtggtacatt ctcaatacga
     1981 tccgttgcag catgtaaaag aaattctctt tggatctggt cgtcagtcca ttgaacgata
     2041 acctcagcgg ctcctagttt ataagcttct tgtgtgatca atcgagcaag gggtgcctgt
     2101 tccacgctga tctgtaaaac aactgtgtgt cctttttctg tggctacgcc agtttctgca
     2161 attaagcgtg catatttttt tagaagttca ttaaaatcag ataacataat aaaaattcct
     2221 cctcaccatt ttttctggta actattagca tgataccatt taattatgat tcaaacaatg
     2281 aggctttaaa taaaaaaagg aggttatttt tttaaggtta agtttcatga tatatgttac
     2341 tacttgctat ttcagcttgt gaagaaaatc aaaaactaat tttgcatatc aaaaaaacat
     2401 gttacaatga aattaatcaa tggtggaagc gttataaaaa aattaaggag attaaaaaat
     2461 atggcaagaa aaaagacgat tactagagaa cagattttgg cagctgctta tgaagtagct
     2521 gccaccgatg gatttacacg atttactgcg agaaacatcg cgaacaaaat gaattgttcc
     2581 acgcaaccga tttatcttga gtttaaaaat atggatgact tgagacaagc tctttttgag
     2641 cagatctatg atcatttaca atatgaagtg ttcactgttg agcatacagg gaatacgatt
     2701 atcgatcttg cttttaatta tatccatttt gcaagaagag agaagaagtt gtatcgttca
     2761 ctgtacttag aagaatatgg tggaggaaaa gagatgcaga atttctctta tacttacttt
     2821 gtgaatacag tgaaacgcga tccagcttat gtgaatcttt cagaagagca gatcgaatct
     2881 ttgtataatg ggacatggat catagctact gggatcgcag ctttgatgtc ttcaagcatc
     2941 atccatccaa ctgatgaaca gattgaaaag atgatccaag acagtataga tgctatcttg
     3001 aaattcaaag aaccaatcga aatcgattga atatactcaa aagttccaca aatcagtagg
     3061 taacaagaga tgtgtcaaaa taaggaaaag ttcgtttttc ttacagcgta tgcattttta
     3121 actcccgaag aactcttcca tgaatgcaat gaataaaaga gccatacaga ttgaataaat
     3181 ctggatggct cttttattgc aagtatatta aatttttgcg actagttctt tagcgaaagc
     3241 ttctaaattt tcgatatctt cttcttcagc attcagatcc actttaacat tttcggctcc
     3301 ttttgatgcg cctgtttttg caaaagcagc atcgaaatca tccaccgatt tgcagaattc
     3361 atcgtaaaaa gtatcaccag atccgcagac accgtaaatt tttcccaata gatccaattc
     3421 ttgcaagtct tcataaaagt ctacgatctc atcaggcaga tcgccatcat cgtatgtata
     3481 agtagctact acgcaaatat ctgcctcctc gaattcatct gcatctactt gggtacattc
     3541 gttgatttcg acttcgatat ccagattttc aaatgcttct gcaacaatat cggcgatttc
     3601 ttcagtgttt cctgtcatgc tagcatatac gatttttgct aaagccatga tgtgcctcct
     3661 cgtttataat tatttaacaa ttatgaatta taccaaaaca tcattgaaaa gaaaataaaa
     3721 cctaaagaca cctggggaat gtcatttggc taaatgtatg ccgctatgtt aaaatgagag
     3781 ggatttgtaa aaaggagacg aaaatatgat tactttaaaa tcagcacgtg aaattgaaat
     3841 gatggcagaa tctggagctt tattagcaga tgtacataaa aatctgcgtg attttataaa
     3901 accaggaatc acaagttggg atattgaggt atttgtcaga aactatatcg agagtcatgg
     3961 cggaattgct gcgcaaattg gctttgaagg gtatgaatat gcgacttgta tcagtataaa
     4021 tgatgaaatt tgtcatggat tccctcgtaa aaagcctttg aaaaacggtg atttggtaaa
     4081 agtcgatatg tgtatcgatt tgaaaggcgg catttctgat tcttgttggg cctatgtagt
     4141 tggagaatct acacctgaga tcgaccgatt gatggaagta acgaagaaag ctttgtacat
     4201 cggtattgag caagcacaag tagggaatcg gattggtgac atcggtcatg ctatccaaac
     4261 ttatgtagaa ggcgaaaatc tagcgatcgt tcgtgatttt attggtcatg gcgttggtcc
     4321 tacgattcat gaagaaccag cagttcctca ttacggagaa gctggaaaag ggttgcggtt
     4381 gaaagaaggc atggttatca cgatcgaacc tatggttaat accggaactt ggaaaatgaa
     4441 aatggatcct aacggctgga ctgcgtatac aagagacggc ggactttctt gccaatatga
     4501 acatacattg gctattacta aagatggtcc aagaattttg acatcacaag gagaagaagg
     4561 cacttattga tctatccagt caataggtac gggagaggta catgaaagtt tggaacaaaa
     4621 taaaacaaaa tgaaaatctg atgcgcttta ttgagacgac tcaaaagcgc atcactgatt
     4681 cagaaatgag tacgacctct gtcgtcgttg cttattattt actcttgtct ttatttccat
     4741 tgattattgc ttttggtaat attttaccgt ttctccacat cgatcaagag actgtactga
     4801 cctatttacg agaagtgatt cctgagaccg tttaccagtt tatcggtcct gcaatcaaag
     4861 accttttgac acaaagttca ggaggcttgt tatccgtttc tgcattagcg gctttatggt
     4921 ctgcaagtca aagtatcaat gctttgcaaa ttgcgatgaa taaagcgtat ggcgtagaaa
     4981 atagaaaaaa ttttttgatc gtccgtttct tctcactaat tgtcattgtc ttgttcatga
     5041 ttgctattag tggggtaact atcgttcttg ggttgggaca gctgattttg gatgctattc
     5101 agccaatcgt acaaataccc gtgaacttta tcgatcagtt ccaaactttg aagtggccaa
     5161 tcacattggt agctttattt gtcatcatgt tcttgattta tttaattgtc cctaatgctc
     5221 agttgaagtt gaagacgatc attccgggca cgatcttcgc tactgtcggc tggatgctgt
     5281 tatcacaagt gtttagtatc tatgctaggt attttgcaac gaggatcagc ggttatcaga
     5341 tcataggaag ttttattgta ctcatgcttt ggcttaattt tgctgctacg atcatcattt
     5401 tgggcggaat catcaatgca gttgttcaag aatacgtgac gggtcatcaa atcaaaaaac
     5461 gtcgagatcc aagccgaaaa tggttcaata aattgaaaac taaattttct cataaaaaaa
     5521 agtagaaaca agagttctta ttgtaaattt atatcagtaa tttataataa ttatgactct
     5581 taattaaagt aggtgcccat atgcttgttt cttttagtat ggttataaga ttttttctga
     5641 caattttatt atcactgata ttaacacctg tattcaaaat catttcagta caaacaggta
     5701 tggtcgacaa accaaatgaa agaagaataa ataaagttcc aatgccttca gcgggcggac
     5761 tgcctatctt tatttcattt gttatatcga ctttattctt attccgtaaa attatccctc
     5821 aatcgtatat tgtaccgatc ttgattgctt ctttagtaat catagtcaca ggagtgctcg
     5881 atgataaata tgagctgaca cctaaacaaa aaagtatagg gatcttgatt gcttctctta
     5941 ttatttattt tgtagcagat atacgtattg attccttcac gcttcctttt attggatata
     6001 tccaactagg ctggcttagt tttcccgtaa cgattttttg gatcttcggt atcacgaatg
     6061 cggtcaatct gattgacggt ttggatgggc tggcggcagg gatttcattg atcggtttaa
     6121 ttacgatagg aatcattgga tattttttcc tccatgcttc tacagtgtat attccagttg
     6181 ttattttctg tcttgtagca agtattatcg gtttttttcc ttacaatttt tatccggcaa
     6241 agatttattt aggcgataca ggagctctat tcctagggtt tatgatggcc gtcctttcac
     6301 tacaagggct gaagaacgtg acttttgttt catcgatttc tttgctgatc gtcatgggag
     6361 tgccggtaac agacacgttt tttgctatca ttaggagaaa agcaaaccgt gtttcttttt
     6421 ctacagcaga taaaaaacat cttcatcacc gactaatcag tttaggcttt actcataaag
     6481 gtgctgtcct gacgatttat gcgatggctt tgatgttttc tttcacagct atggtcatga
     6541 attatactgg aagaatcgga acgatcatat tgattattgc catgttgttt gctgcaattt
     6601 tattatttga attgattggt ctgatcaatg agaaacatca gtttatttta aaaacattgc
     6661 gatttttagg gaataaagag taccgtacgc aagtaaaaca gaaatatttg aagaaatgga
     6721 accgtcatat agatgaataa caaattaaac cagccgatcc ttattagtat tgtgacggca
     6781 aacagtgata aaatttttcg gacactagat aattttattt caactgtaaa agatgaaaca
     6841 gcagtaaaaa tccgaatttt tgacaacaat tcacttcccg agtacaaaaa aagactaaca
     6901 gagtatagga aatatcccta catcgatatc tattttcatg atgaaaattc cggatttggt
     6961 tatggacata atcacaatct gatagctagc gattctcgct atggcattat ttgtaaccca
     7021 gatattttag tggatgaaga gacagttaca gcattggtct tgttattgaa agaacatccg
     7081 gaatgtgcga tgttagcacc taagattctt aatgaagatg gaacaactca gcacctcatc
     7141 cgtgagcagc tggctgtttt tgattacgtt ttgcgctttg tgcctttcaa atgggtcaaa
     7201 aaaaagtttg acaaacgact ggctgtgttt gaatgccgag gattgcctac tgatgctaat
     7261 agttatgtac ggatgatctc aggtagtttt atggtagctg atattgataa atttaaagag
     7321 ataaaggggt ttgatgagcg tttcttcatg tactttgaag acaacgactt gtgtttgagt
     7381 tttgaaaaag caggttacaa gttattgtac acaccctttt atgcagttat ccatttttac
     7441 gggaagggtg cgcatcgaaa tgcaaaactg tttcgcgtgt ttttgacttc tatgatgaaa
     7501 tttttcaata agtgggggtg gcgttttttc tgatgaaacc agaaatatta gtagtcgttg
     7561 tgctttatca gcaactattc agtcaatctc cagcttatgg accattaagc aaagcactta
     7621 gcgaaaagca aattcagctt attatttacg ataatagtcc tcaaaggcag caaaatgagc
     7681 tgtttgaaaa agaaaatatc gtttatcatc atgatccgca aaatcctggt ttagcggtgg
     7741 cttataattt tgcgttgtct caagcagatg aaggaacacg ttgtctcgtc actctggatc
     7801 aggatacgag gctagtcgac gattattttg aactattgcg taaagtaacc tttacagatg
     7861 aatgtgttgc tgcagttccg atgatttttt ctgggagccg acaagtttct cccctatatt
     7921 cagatcgtta tatcaatcgt acagctcaag cagtagaagt tgaaggccca acagctgaac
     7981 gaatcatggc aatcaattca ggtgttgcct ggtctgtcaa atttctaaaa aagattggcg
     8041 ggtttaatcc tgctttttct ttagattttt tggatcattg gctgttttgg aaagtggatc
     8101 agttggaaaa aactgtggag atccttcctg tacgaatgga acatgatctt tcggttttag
     8161 actatgaaaa agtttcaagt aatcgatacc gttctatttt acaagcagaa aatcgatttt
     8221 atcatgaata tgatcaaagg catttatcca gtcaccgacg acaattattg ttaagaacag
     8281 ctaaacagtt cttgaccgtc aaaaaccggc atatctggcg gatgactttg cgctcatatg
     8341 ttaagaattg gaaggtgtga attcgtgcgt tcggtttgta tagctacctt taatggaggc
     8401 agatatttaa gagagcagtt agatagtatt ttatctcagc tttcagcaga agatgaagta
     8461 gtcatttctg atgatgggtc agtagatgat acatgggata tattaactga atacgcagaa
     8521 aaagataagc gaatccagct tttttcgggg cctcgtcaag gattgatcgc taattttggt
     8581 tatgcgatcc agcagacgaa aggagatcta atctttttgg cagaccaaga tgatgtttgg
     8641 ttggagacaa aagtgaaaaa gatcactgac tattttgagc agaatccgga tcagctagta
     8701 gtagtttctg atttaatcat tgtggacagc gagctgcaag agatccatga ttcgtatttt
     8761 tcgtttcgaa attccaaaga aggatggctt aataatgtga taagaagtca ctatattggt
     8821 gctggtatgg ctttccggtc ttctttaaaa cccgatattt tgccgatccc gtcagaagtg
     8881 ccgatgcatg atatgtggat tggtttgctg ggaggaaaga agactgggtt cctaagagaa
     8941 ccgttaactc tgtaccgaag acatgattta aacgcgagtg agattgaaac acattctaaa
     9001 ttacggcaaa aaatttcttg gcgtgttcat ctgattacgg cgttgatcaa acgaaaatgg
     9061 tttaaacgtt gataaaagat gtttaaatcc ttaatatttc catgacaaat ataacaattg
     9121 atgagataat aacaaagtga aatttgaaat caaaaaggag agcagatatc catgaaggga
     9181 atcattcttg ctgggggcag cggaacacgt ctgtaccctt taacaaaggc aacatcgaaa
     9241 caattgatgc cgatttatga caaaccaatg atttattatc caatgtctac tctgatgttg
     9301 gcgggaatca aagaaattct gattatttca acgccacaag atacaccacg tttcaaggaa
     9361 ttatttggaa acggaaatga tttaggactg cagatcgaat atgcagtaca agaaagtcca
     9421 gatggattgg cacaagcgtt tatcattgga gaagaattta ttggagatga tagtgtctgc
     9481 ttggtattag gggacaatat ttactatggt ggcggtctgt ctaaaatgtt gcaacgagca
     9541 gcttcaaaag atacaggcgc gacagtattt ggctatcatg tcaatgatcc agaacgcttt
     9601 ggtgtagtag aatttgatga gaatatgcaa gctttgtcga ttgaagaaaa gcctgctcag
     9661 ccaaaatcaa attacgctgt aactggcctt tatttttatg acaatgaggt aatttctatc
     9721 gctaaaggta tcaaaccttc agaacgtgga gaactagaga ttacggatgt caataaagca
     9781 tatttggata aaggaaaact atccgtagaa cttatgggac gcggatttgc ttggcttgat
     9841 acaggaacac atgaatcatt gcttgaagca tctaccttta tcgaaacaat tgaaaaacgt
     9901 cagaacttaa aagtggcttg tttggaagaa attgcttacc gtatgggcta catcgataag
     9961 gaacaattgc tggcccttgc acagccattg aaaaaaaatc aatacggtca atatttattg
    10021 aatttggccg ctgaataagg agtccttaca gatgaaagta acagatacaa aattaacaga
    10081 tgtcaaaatc gtagaaatgg acgtctttgg cgatcatcgc ggtttcttca cagaaagtta
    10141 ttcaaaagca aaatttgcag aacatggatt gaaatatgac tttgttcaag acaaccattc
    10201 actatcagct gaagctggtg tccttcgggg gcttcatttc caaaaaggag aagctgcaca
    10261 gacaaaattg attcgggtag tcactggagc agttttagat gtagtagtgg atatccgcaa
    10321 aggtagcccg acttatggtc aatgggacgg atttatctta tctgaacata atcatcgaca
    10381 gttgcttgta cctaaaggat atgcccatgg attcgttact ttgacaccaa atgtgaactt
    10441 cctttataag tgtgataatt actataatgc agaagcagat gggggcatcg catttgatga
    10501 tccagattta gcgattgatt ggccaattga tccatcaaga gcgattatgt cggaaaaaga
    10561 tcaccgtcat ccgacattaa aagaatttga agcagaaaat ccatttgttt acggagaaat
    10621 ctaagaatta aggagaaatt ttcttatgaa aaacatcatt gttaccggag gtgccggatt
    10681 catcggctct aatttcgttc attacgtggt aaacaatcat ccagaagtgc atgtcactgt
    10741 tttggataaa ctgacttatg caggaaacaa agagaacttg gccggacttc ctagcgatcg
    10801 cgtagaattg gtcgtaggag atgttgcaga tgcagaatta gtggaccgtc tagtaaaaga
    10861 agcggacgca gttgttcatt acgcggcaga atcccataac gataattcat taaaagatcc
    10921 tttcccattt gtacaaacga atatcattgg tacgtataca cttttagaag cttgccgcaa
    10981 gtatgatgta cgctatcatc acgtatctac tgacgaggtt tacggcgatc ttccattacg
    11041 tgaagacctg ccaggacatg gggaaggcga aggagaaaaa ttcactgcgg aaacaccata
    11101 taatccttca agtccatatt cttctacaaa agctggttca gatctgttag taaaagcatg
    11161 ggtgcgttct tttggcttac gtgcaaccat ttcaaactgt tcaaacaact atggtcctta
    11221 tcagcatatt gaaaaattta ttcctcgcca agtaacgagt atcttaagcg gtattcgtcc
    11281 aaaattatat ggcgaaggaa agaatgttcg tgactggatc catacgaatg atcattcttc
    11341 agcagtgtgg ctgattttga cgaaaggcag aattggcgaa acctatctga tcggtgcaga
    11401 cggtgaagaa gataataaga ccgtgatgga attgatctta gaaatgatgg gccaaccaaa
    11461 agacgcatac gaccatgtaa acgaccgcgc agggcacgat ttgcgttatg caatcgattc
    11521 gacaaaattg cgagaagagc taggttggca gcctgagttt acgaacttcc gtgatgggtt
    11581 ggctgacacg atcaaatggt acgaagaaca tgaagactgg tggaaaaaag aaaaagaggc
    11641 cgttgaagca gcatatgcca aaaatggaca ataatacaga ggaaatttca ctgtattaat
    11701 ggaaaaacac atgtgaggca ggcgctcgcc tgtctctttt ttaaaataaa aatcaaggag
    11761 cggataacca tatgttttta ttaactggag gaaatggaca attaggtaca gaattgcgcc
    11821 atttattaga tgaaaaaaat ctgccatatg tttcaacaga tgcaaaagac ttggatatca
    11881 cagatgctga gaaaacaatg gcttatatta cagaattaaa acccgatgtt attttccatt
    11941 gcgcagctta cacagcagta gataaagcag aagaagaagc aaaagaatta gatgagaaaa
    12001 tcaatgtcga tggcacaaga aatgtagcac ttgcagcaaa agctgtgaat gcacaattcg
    12061 tctatatcag tacagactat gttttcgatg gaaaaaacaa aaatgtagaa tacgaagtag
    12121 acgaccaaac gaatccacta aatgaatatg gtcgtacgaa attattaggc gaaaaagcag
    12181 tagaagagat tttagaagac tattatatca tacgaacttc ttgggttttc gggatctatg
    12241 gacacaattt cgtcttcacg atgcaacgcc tagcggaaac ccgcgacaaa ctgactgtag
    12301 taaatgacca gtttgggcga ccaacttgga cacgtacttt ggcagaattt atggtatatg
    12361 tcatcgaaga aaaagcacca tttggtattt atcatttgtc gaacgaaaat agctgttctt
    12421 ggtatgagtt cgcaaaagaa atattgaaag atacagatgt agaagttgca cctgttacgt
    12481 cagaagaatt tccgcaaaaa gcaacacgtc cgcagtattc agtcatgagc ctgaaaaaga
    12541 cagaagcgtt aggttttgtc ataccaactt ggcaagaagc acttgcgcaa atgctggaag
    12601 ctgccaaaaa aatgaataag taatttcctt tttatcaaat agaatttgga gcatgatatg
    12661 gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa aagcgaatca ggcagattcg
    12721 aatttggtgc ttgacctgtt tctaaaactc cgctatctta ttggcgtttt agtcatcgtg
    12781 gtcatcgtta cttttaattt aaacggaagt tcacttggtg tttgggataa atatgtgtcg
    12841 caaagagacg atgggaaaaa aactgatgtg atttttggac agaatcggga agtgcggtca
    12901 gacgaatggc tggtgcagac acctttttac ctctcacaag ctgaagagga cttttcgttg
    12961 gtgaacgagg attattcatt aaacggacaa aacatgatca ttgcgtataa ttctcctgta
    13021 aaggatatta cggttatcgg aaaaccattc aactggggat tctttttcct cggaagagat
    13081 cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca tggtcttgct tggattcgaa
    13141 gtactgatga tattaacgaa gaggaaaaaa gctctatcgc taatcggtgc atttgcgctg
    13201 acattttctc cagcagtcca gtggtggttc atgcaacatg tgggcgattt gattttcttt
    13261 actttgggac tgatgattgc tttttatcat tatttctaca atcatgataa aaaatggcta
    13321 cgaacgttga tgatgctttt ggtggttatt ttcggtattg ggtttatttt agtgatttac
    13381 ccagcccatc aagtgatgct ggcgtacttg ctgatttttt attttgttgg cctgcttatt
    13441 tactatagca agaaagtttc atgggatctg ttggatgtag tgttgattgg aggggcagtt
    13501 ttatttattg gcggagtcat ggttcatttc tggctaacgt caaaagacgc attgttagct
    13561 tctttgaata ccttatatcc aggaaaacgg gtaagtactg gaggaaagtg gacgattggc
    13621 gaattttttt ctttccttac aaattggaaa attccttttg aagatatcaa ctattcgaat
    13681 aactctgaag tggccctgtt ctatcacttt tttccaactg tattcctagc aagtccattt
    13741 gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg gacgtatttt gatgattttt
    13801 tgtctgtttt ctatcttttg gattaccgtt gggttgccaa aatggctagc agaaatcact
    13861 ctgctgtctt atgttcctcc attaagagcc atccttactt ttagttttgc ggcttgttta
    13921 ttatcgatct ggtttatcgc ttatatttgg agagaagatg ttattcctat ttggtataaa
    13981 ttcattttgt tggcagtaaa tgcatggtct tatttctatg cgattacgca ttcgaaaatg
    14041 gataattatt tttcggattt tgaaaccatc gcagttgctg tgttgggaac agtgatttta
    14101 gcttttgcat tatttggctg gagacgcttg ttttacctcg cttttgtcgc cttagttatc
    14161 gtatctggct ttttcgtcaa tccagtcgta gaaggtacag gagcgatttt tgaaaaaaca
    14221 ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg gtaaagtttg gttgtctgaa
    14281 gatgaattat ataacttcac cccaacattg ggagtgaaat ccttcaatac agtacgcttt
    14341 tatccagata tagctgcatg gaagaaaatc gatccgaaag aagaatacga gaagtattat
    14401 aatcggtatg ctcatacaag agcttttatc gctaaggaag atacaaagtt cattttagat
    14461 cgtccagata tgttttcggt cacgttgaat tttgaagacg cagaaaaatt agggatcgac
    14521 tatgtgatca gtaaacgacc attaactgat tacaaccaat tatatgaagc gcagttcacg
    14581 cagctatatg gaccggataa agacggttat atgattttta aagtagaata tccggattat
    14641 ccagatgtac cacaaacaca aactcaggaa aatcagctag tcgtacagta ataggctagc
    14701 tgagaataat cctgaaatat tagtagaaag gagagtgaag ataattcaag caatcgtaca
    14761 aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg ttgctgacgc tagtcttttt
    14821 tgtttggaca gcaggggtag gttttccagc aactttgttt atatataaca atagttcatt
    14881 acttatcgct tttagtgtgg ctattttgct cattttaaat gtttataaag caaaaggcgt
    14941 cgattttctt tatctaggtg ttgcgcttgc ccttttcatt ttcttttctt actttcggga
    15001 attaagaaat gagtctttat ttggagactt actgtttcct ttgattattt tattttgttt
    15061 agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt atttatctga ttgtctttac
    15121 ggtcttcctc ggaatcacgt tatatcgtgt atttacagag atccaagtac cagaaggaca
    15181 aagcatctgg gctccaggca attcgctttc tgatatttgg atcaatacga atacaatcgg
    15241 aagttctttg atgactcttg ctttattgat cagtggattt gccagttctt ttgaacgatg
    15301 gtatgttcga atcatcggtg ggatagcagt cgttgcagcc tttttaggta tttgggtctg
    15361 ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg atattggatc ttttacctaa
    15421 atcgttgatg cgtgtcattc gtgcgccatt tattgcctac acggttacga ttcttttagc
    15481 tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc aatttgttta ccggtcgaga
    15541 agatatttgg cataaattct atcagacgct aggagaaaaa tctgaacaag ttttactagg
    15601 aatgaaaaca tttatcttcc agcgaggaaa ccaattttta ggaaaccaca atagttataa
    15661 ttctattttg aatatctatg gattgatagg atttgggatt gctgctttgc tgcttatcct
    15721 ttttattggt cgcttaacgt tgaaagcaga tttatccaac gggcagatga cttttatctg
    15781 ggcctttttt gcggtgatga tgcagtcatt tatggaggat acattgactt catacccatg
    15841 ggttccgatt atttatatcc tgttggctat ggccgttcat cgatatgatg aaccagaagt
    15901 tacggaggaa ttctatgtta tggaagaggc atatgacgat atgacttatg atgatacatc
    15961 ttatgaggaa caaccagata gcgctcattc ttccaggatc caacgctatc attgataggc
    16021 acaaaatacg agagaggctg tgacaaccgt cgcagccttt tttggtttat tgcaattgtc
    16081 tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg tatcatagaa aattaagaaa
    16141 gaaataagaa cgcagctcgg taccaaaaat atggtaaaat ggcacttgac tgtaattcac
    16201 tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt atttatttat gaaggacatt
    16261 tatcagaaca gaaaattact gctacagttt tcgatcaatg acttcaaatc aagatatgca
    16321 ggatcgtttt taggaatctt atgggcgttt atcaatccgc tatttactgt attgatttat
    16381 tggctggtat ttggtgttgg actgaaagca caaatgatcg atgcaaaata cccatttatc
    16441 gtatatttag ttaccgggat gattccctgg ttcttcttct cagatgcatt tgcatcaact
    16501 acggttgttt ttcgggaata tacttattta gtaaagaaag tcgtattcaa tattcgtatc
    16561 ttgcccacga cgaaaatcct atccaatctg tacacccatt tattcttcat tctgattggc
    16621 atagctgtcg cgcttataaa tggtatatat ccttcagcaa tgtctttaca gctgatttat
    16681 tatttatttt gcatgtgtgc tttcttgaca ggattgactt ggctgacggc atcgatccaa
    16741 ccatttttac ctgatatcat gcaattaatt acgatcgtgc tgcagttgat catgtggaca
    16801 ttaccgatat tatggagccc taatcagttt gattcaggtc ttgtaagcat attgaaatta
    16861 aatccgctgt actatgttgt tcaaggttat cgtgagtcat ttttaagtga agcttggttc
    16921 tgggagcatg gaacatatac tttgtatttc tgggttttca cgttagtaat gttcgtaatc
    16981 ggctctgcag tgttccgtag attgaaacca catttttcag atgtgctgta aggagaatag
    17041 ctatgacaga atatgcaatt agactaaaag atatcacaaa atcttataat atgtacgcaa
    17101 aaccttcgga tcgatttcgt gaagccctca atccttttaa gaaatcctat catgatgtat
    17161 tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg tgagatgatc ggtttcgtgg
    17221 gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat tacgggagta ctgacaccaa
    17281 catccggcac gatggaaatc gaaggaaaaa tctcagcttt gctagaacta ggatcaggat
    17341 tcaatcctga gtattctggc tatgaaaata tctatttaaa tggaatggtt ctaggatttt
    17401 caagagaaga agtcgatgaa atggtcgatg atatcattga atttgctgac atcggcgacc
    17461 atatttatca acctgtaaaa acctattcaa gtgggatgtt cgttcgtcta gcttttgctg
    17521 tagcgatcaa tgtgaatcct gatattttga ttgtggatga agcattggca gtaggagatc
    17581 ttgaatttca actaaaatgt atggaaaaat ttacagaaat caagaactcg ggaaagacga
    17641 ttttattcgt ttctcatgat attagttcga ttcgccgttt ttgtgatcgt tcctattggt
    17701 tgaaaaatgg aaaagttgtc gaatatggtg atacgatgga agtaacgacg aattacgaaa
    17761 actttttgaa gaaaaaatca gttaaaacag tagatcgaaa taaaaaccaa gtagaacgct
    17821 tcgcagctcc agatatcgta gacgtgacga aagccgaatt gttggataaa gataaaaatc
    17881 cagtggatat gctggaacaa ggagaaaaac tatacgttaa agtaacctat gaagtaaaag
    17941 acgatacaat caaaaatcca gttttaggaa ttgctctgcg tacagtcgac aatttttatg
    18001 tgtgcggact caatactcta ctagatggta tcaagatccc ttggaaaaaa ggtgagaata
    18061 tcttttatct ggagtatgaa aagttaggtt tgttagctgg tgaatattat tttgacgttg
    18121 cggtctttga agaaaatgca acggttcctt tggtatacaa gacaaaatac atgaacttgt
    18181 ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt agaccataaa tgggaagaag
    18241 ggactcatag caatgaaata tgattttgaa atggacctag atgaacaaag cagtgtggga
    18301 aaaattgccg cacagatcaa accaggaagt aaagttttag agtttggtcc tggaaacggc
    18361 cgattaacga aacatttgat cggagcaaaa caatgtgaag tgagcattgt cgagctagat
    18421 aaggaattgt tcgattttgt cagcgagttt gcccaagatg gattttatgg agatatcgaa
    18481 agttttgaat gggcaaacta ttatgctgga caaacatttg attacgtttt gtttgccgat
    18541 gttttagaac atttggtaga tcctggaaaa acgttgaaga aagttagaga atttttgaat
    18601 gaagaaggag aaattctaat tacattccct aaccttgcgc ataattctgt catgattgat
    18661 ttgttcaata atcaattgcc atgggcttct tatggtttgc tagatgaaac ccataattct
    18721 ttttatactc atgatggctt ccaaaaagtt tttgaaaaag caggattata catcaatatt
    18781 gaagattatt tgtacttagc tgtaggtgat acagaattaa aatcaactta tgaagaattg
    18841 ccggaagctg tacgttatga tttcaaaatg cgaccatttg gcgaagtcta ccaatacttc
    18901 ttctcattga tgaaacatcc agtagcacaa agttccattg cgcagccgca aaattctaat
    18961 tatgtgaaag tattagaagt aatccagcaa acgaaacaag atgaaataat tcaacaaata
    19021 ccattcaata atttcacagg ggaaaatgag acactaagtt tccatgtatc agaattagca
    19081 gaacgaatgg tgtttcgctt cgcgcagcaa cctagcttta tcgaattcag cgcagaagct
    19141 gctggagaaa aactcacatt tatcgattca aatgctgtag tcaaaacagt aaatgattgt
    19201 tatttgtttg atggtaaaga tttaccagag ttctttttaa cggatattgc agggaaagaa
    19261 gtgactatcc attgtcacta tcgttttatc ggagaattga caccaacaat gaaagaattg
    19321 ctggaaacaa tccgtccaat ggctgaagtt acaaagcagc tttctgagaa aaatgatgaa
    19381 ttagaaagag aaaatcatta tcttattgag aataatacaa gattagatca gacgttgaaa
    19441 acaaccataa atcgctattg cacgttgttg gaaagtgatg aatggacaat caaacataga
    19501 ttagggcgac gtaagaaaga aacatcgaaa aaaatccaag aaaaagagct ttctctatgt
    19561 attgacgaaa aaatctggga tgcagaaaca aagatattga aaatcatcgg gtggggaatc
    19621 gctaattcgg atcgccaacc attgtcctat aagttatcag ccgaccaatc accatttttt
    19681 gaagcaattc ctgttttacg agaagaagtc aatcaagcaa aacagctagc tgaaggagca
    19741 aaagcaggat ttgaactgcg gatcctttgc gagaaggaac gttcattctt agttgaagca
    19801 gtggctcaaa atggtcaatc atggatcatt gaaatgtaaa taatgattaa cttataaaga
    19861 taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt atttatcgat caaatcactc
    19921 gtgaacgggc cactggcgat ttgcttattg tgggctgggc aattgatgaa gtgaccaaag
    19981 aaatccctac gataaaggta gaaaaagaaa atgtgattgc tgaagcaaca catgtcgtaa
    20041 gattggatat caatcattta tataatcttg atgttaagac ccaaagcggt tttaaggtac
    20101 gtttatcagg gaaaatgaga ggaaaagcaa tcttagactt ccaaacagca agacatcaaa
    20161 atggcatagc tgtgaaatta aacggtaaat atccttatga tgacggcatt gaatcttcat
    20221 gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta tgcaaggact catggcgtaa
    20281 aaaaagcaat ccgccgggtc aaactggaat tgaaaccagg atcgatcgac tatgcagagt
    20341 ggatcagtcg tcatgagaaa cctgatctga aagagcaaag gaaagaagta caaggatttg
    20401 cctaccgccc attgatttcc atacttatgc ctgtctataa cgttgaaatc aaatggctcg
    20461 aaaaatgcat tgattcggta cttgatcaaa cgtatgatca ctgggaacta tgtatcagtg
    20521 atgatgcttc tactgatcca gctatccgaa agtgtctaga atcctatcaa gcaaaagatg
    20581 atagaattaa agtcgttttc cgacaagaaa atggacacat cagtctggca actaattctg
    20641 cattgaaaat ggcagaagga gagtttatag cgctcttaga taatgatgac gaattacctc
    20701 catttgcctt atatgaagtg gcaaaagtgc taaacgtaca tccagaactg gatttgatct
    20761 acagtgacga agataaaatc gatgcagatg ggaatcgctt tgacccgcac ttcaaagcag
    20821 actggtctcc cgatacattg atgggaaata attatatctc tcacttaggc gtatatcgga
    20881 caagcattgt taaggaactt ggcggtttcc gtaaaggata tgaaggctct caagattatg
    20941 atttggttct tcgtgtgaca gaacaaattc ctgcagaaca catttaccac attgaccgtg
    21001 tcttgtatca ttggcggacg atcccgggat caactgcaag caatggagaa gctaaaagct
    21061 acatctatga ttctggagtg aaagcattga cagatgctct atctagaaga aatatcaagg
    21121 gaagcgtaca tcctggcaga atttctggtt tttatgaaat cacttatgat gttttgcaag
    21181 aagacttggt cagtgtgatc attccaacta agaatggcta tgaagatcta aaaacttgtg
    21241 tggattcgat catcgaaaaa acgtcttatc caaattatga aatcattatt gcagataatg
    21301 gaagtacaga tcctaaaatg caagaattat ttgcagaata caagcatcag ctgaaagacc
    21361 gatttatcgt cgaattaatt gacattccat tcaattattc acgtatcaac aatctggcag
    21421 cagaaaaagc aaacggcaaa tactttttat tcttgaataa cgatacagaa gtgattgaac
    21481 ctgactggat gacagcgatg gtttcttatg cacagtttga tcgaatcggc tgtgtaggag
    21541 caaaattatt ttatccggat gatacaacgc agcatgcagg tgtattatta gggatcggtg
    21601 gcgttgcagg acatgcattg aataactatg atcgaacaca ttgtggttac tttggccgat
    21661 tggtcattga cgtcaattac ttagcagtca ctgcagcttg tatgatggtc aaggcagctg
    21721 acttcaatgc tgtcaatgga tttgatgaaa tattagaagt agcattcaac gatgttgact
    21781 tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta tgcacatcaa gcagaattgt
    21841 accatttcga atctaaatca agaggttatg aagatacacc agaaaaacaa agacgttttg
    21901 ctggggaaat caaaaaaatg caagataagt ggccttctta tattgctcat gacccatttt
    21961 ataacgataa tctaactaaa gaaggaatcg gcgatttttc gttgagacct gattgattaa
    22021 aagaactgta ttttcatttt tattaatgaa aatacagttc ttttaattta att
//
LOCUS       DANKEF010000045        20802 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000045 DANKEF010000000
VERSION     DANKEF010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.045"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            319..627
                     /gene="rpsJ"
                     /locus_tag="PWZ48_001929"
     CDS             319..627
                     /gene="rpsJ"
                     /locus_tag="PWZ48_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDL2261582.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            658..1287
                     /gene="rplC"
                     /locus_tag="PWZ48_001930"
     CDS             658..1287
                     /gene="rplC"
                     /locus_tag="PWZ48_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDL2261583.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            1315..1938
                     /gene="rplD"
                     /locus_tag="PWZ48_001931"
     CDS             1315..1938
                     /gene="rplD"
                     /locus_tag="PWZ48_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDL2261584.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            1938..2228
                     /gene="rplW"
                     /locus_tag="PWZ48_001932"
     CDS             1938..2228
                     /gene="rplW"
                     /locus_tag="PWZ48_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDL2261585.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            2267..3100
                     /gene="rplB"
                     /locus_tag="PWZ48_001933"
     CDS             2267..3100
                     /gene="rplB"
                     /locus_tag="PWZ48_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDL2261586.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            3143..3421
                     /gene="rpsS"
                     /locus_tag="PWZ48_001934"
     CDS             3143..3421
                     /gene="rpsS"
                     /locus_tag="PWZ48_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDL2261587.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            3444..3791
                     /gene="rplV"
                     /locus_tag="PWZ48_001935"
     CDS             3444..3791
                     /gene="rplV"
                     /locus_tag="PWZ48_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDL2261588.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            3805..4461
                     /gene="rpsC"
                     /locus_tag="PWZ48_001936"
     CDS             3805..4461
                     /gene="rpsC"
                     /locus_tag="PWZ48_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDL2261589.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            4464..4898
                     /gene="rplP"
                     /locus_tag="PWZ48_001937"
     CDS             4464..4898
                     /gene="rplP"
                     /locus_tag="PWZ48_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDL2261590.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            4888..5076
                     /gene="rpmC"
                     /locus_tag="PWZ48_001938"
     CDS             4888..5076
                     /gene="rpmC"
                     /locus_tag="PWZ48_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDL2261591.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            5101..5367
                     /gene="rpsQ"
                     /locus_tag="PWZ48_001939"
     CDS             5101..5367
                     /gene="rpsQ"
                     /locus_tag="PWZ48_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDL2261592.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            5425..5793
                     /gene="rplN"
                     /locus_tag="PWZ48_001940"
     CDS             5425..5793
                     /gene="rplN"
                     /locus_tag="PWZ48_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDL2261593.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            5830..6138
                     /gene="rplX"
                     /locus_tag="PWZ48_001941"
     CDS             5830..6138
                     /gene="rplX"
                     /locus_tag="PWZ48_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDL2261594.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            6166..6705
                     /gene="rplE"
                     /locus_tag="PWZ48_001942"
     CDS             6166..6705
                     /gene="rplE"
                     /locus_tag="PWZ48_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDL2261595.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            6724..6909
                     /locus_tag="PWZ48_001943"
     CDS             6724..6909
                     /locus_tag="PWZ48_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HDL2261596.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            6945..7343
                     /gene="rpsH"
                     /locus_tag="PWZ48_001944"
     CDS             6945..7343
                     /gene="rpsH"
                     /locus_tag="PWZ48_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDL2261597.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            7375..7911
                     /gene="rplF"
                     /locus_tag="PWZ48_001945"
     CDS             7375..7911
                     /gene="rplF"
                     /locus_tag="PWZ48_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDL2261598.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            8072..8428
                     /gene="rplR"
                     /locus_tag="PWZ48_001946"
     CDS             8072..8428
                     /gene="rplR"
                     /locus_tag="PWZ48_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDL2261599.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            8449..8949
                     /gene="rpsE"
                     /locus_tag="PWZ48_001947"
     CDS             8449..8949
                     /gene="rpsE"
                     /locus_tag="PWZ48_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDL2261600.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            8964..9143
                     /gene="rpmD"
                     /locus_tag="PWZ48_001948"
     CDS             8964..9143
                     /gene="rpmD"
                     /locus_tag="PWZ48_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDL2261601.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            9187..9627
                     /gene="rplO"
                     /locus_tag="PWZ48_001949"
     CDS             9187..9627
                     /gene="rplO"
                     /locus_tag="PWZ48_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDL2261602.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            9627..10922
                     /gene="secY"
                     /locus_tag="PWZ48_001950"
     CDS             9627..10922
                     /gene="secY"
                     /locus_tag="PWZ48_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDL2261603.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            10984..11631
                     /locus_tag="PWZ48_001951"
     CDS             10984..11631
                     /locus_tag="PWZ48_001951"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDL2261604.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            11822..12040
                     /gene="infA"
                     /locus_tag="PWZ48_001952"
     CDS             11822..12040
                     /gene="infA"
                     /locus_tag="PWZ48_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDL2261605.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            12073..12189
                     /gene="rpmJ"
                     /locus_tag="PWZ48_001953"
     CDS             12073..12189
                     /gene="rpmJ"
                     /locus_tag="PWZ48_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDL2261606.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            12207..12572
                     /gene="rpsM"
                     /locus_tag="PWZ48_001954"
     CDS             12207..12572
                     /gene="rpsM"
                     /locus_tag="PWZ48_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDL2261607.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            12600..12989
                     /gene="rpsK"
                     /locus_tag="PWZ48_001955"
     CDS             12600..12989
                     /gene="rpsK"
                     /locus_tag="PWZ48_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDL2261608.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            13069..14007
                     /locus_tag="PWZ48_001956"
     CDS             13069..14007
                     /locus_tag="PWZ48_001956"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDL2261609.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            14035..14415
                     /gene="rplQ"
                     /locus_tag="PWZ48_001957"
     CDS             14035..14415
                     /gene="rplQ"
                     /locus_tag="PWZ48_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDL2261610.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            14687..15526
                     /locus_tag="PWZ48_001958"
     CDS             14687..15526
                     /locus_tag="PWZ48_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2261611.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            15502..16371
                     /locus_tag="PWZ48_001959"
     CDS             15502..16371
                     /locus_tag="PWZ48_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2261612.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            16361..17158
                     /locus_tag="PWZ48_001960"
     CDS             16361..17158
                     /locus_tag="PWZ48_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDL2261613.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            17361..18107
                     /gene="truA"
                     /locus_tag="PWZ48_001961"
     CDS             17361..18107
                     /gene="truA"
                     /locus_tag="PWZ48_001961"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDL2261614.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            18772..19009
                     /locus_tag="PWZ48_001962"
                     /pseudo
     CDS             18772..19009
                     /locus_tag="PWZ48_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(19153..19929)
                     /locus_tag="PWZ48_001963"
     CDS             complement(19153..19929)
                     /locus_tag="PWZ48_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2261615.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            20052..20459
                     /locus_tag="PWZ48_001964"
     CDS             20052..20459
                     /locus_tag="PWZ48_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HDL2261616.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT         6976 a         3693 c         4414 g         5719 t
ORIGIN      
        1 tttattgcag gatcaggaaa aaagacagga gtattataaa tagaagaaac acgaaataaa
       61 agagtcagta ttttctaaat gaaactttat gaaaacaaaa tgttttttac aagaaaaagc
      121 cttgctttgt gagtttatat atattataat gacaaaggtg cgtttacttt agtgaattgc
      181 acagacgtaa tcagaatgat ttcgtcggct aagaaacgag aggttgcgac acgcccggac
      241 gctttgccac gaacgagcgt gacggaaatt ttcgtggagc tatgtctact tttcaaaata
      301 ggcgaaggag ggaacaagat ggcaaaacaa aaaattcgta tccgtttaaa agcgtatgaa
      361 caccgtattt tagatcaatc agcggataaa atcgtggaaa ctgcaaaaag aactggagct
      421 agcgtgtctg gtccaattcc attaccaact gaacgcagtc tttacacaat catccgtgcg
      481 actcataaat acaaagattc acgcgaacaa ttcgaaatgc gtacacacaa acgtcttatc
      541 gacattgtga acccaacacc aaaaactgtt gatgctttga tgaagctaga cttaccatct
      601 ggtgtaaaca tcgaaatcaa attataaaac taaaacaaaa ataatggagg tgtactcatg
      661 accaaaggaa tcttagggaa aaaagtggga atgacacaaa tcttcactga atctggtgaa
      721 ttaattccag taactgttgt agaagctact ccaaacgtag tactacaagt gaaaacaatg
      781 gaaacggatg gctacgaagc tattcaagtt ggttaccaag atatgcgtga agttttatca
      841 aacaaacctg cgaaaggtca tgttgcaaaa gcaaacacgg ctcctaagcg cttcattcgt
      901 gaattcaaaa atgttgagct aggagaatat gaagtaggaa aagaaattac agtagatgtt
      961 ttccaagcag gagacattat tgatgtaaca ggtactacga aaggtaaagg attccaaggg
     1021 gttatcaaac gccacggaca aagccgcgga ccaatggctc acggttctcg ttaccatcgt
     1081 cgtcctgggt caatgggtcc agttgcacct aaccgtgtat ttaaaaacaa acgtcttgct
     1141 ggccgtatgg gtggcaaccg cgtaactatc caaaacttgg aagttgtacg tgtcgatgca
     1201 gaaagaaatg ttatcttgat caaaggtaat gttcctggag ctaaaaaatc tttaattact
     1261 atcaaatcag ctgtgaaagc taaataattg taggaagaaa ggaggaacta aggaatgccg
     1321 aatgtagcat tattcaaaca agatggaagc caaaacggtg aaatcacact taacgaagaa
     1381 atcttcggaa ttgaacctaa tgaaagtgtt gtttacgatg caatcgtaat gcaacgtgcg
     1441 tcattaagac aaggaacaca cgcggttaaa aaccgtagcg ctgttcgcgg cggcggacgt
     1501 aaaccatggc gtcaaaaagg aacaggtcgt gcacgtcaag gatctatccg ctcaccacaa
     1561 tggcgtggag gtggcgtagt ctttggacca acaccacgtt cttacagcta caaacttcct
     1621 aaaaaagttc gtcgtttagc aatgaaatct gtattgtcag aaaaagttgc tgaaaacaac
     1681 ttggtagcaa tcgaaggctt gaacttcgac gcaccaaaaa caaaagaatt caaacaagtt
     1741 cttgctaact tatctatcga ctctaaagta ttggttgttc tagaaaaagg taacgacttt
     1801 gctgctttat cagcacgtaa cttaccaaac gtttctgtag taacttctga taatgtgagt
     1861 gtgctagacg ttgtatcaaa tacaaaagtg ttagcaacac aaactgctct tactcaaatt
     1921 gaggaggtgc ttgcataatg aacttactag acgtaatcaa acgcccagtg atcactgaaa
     1981 aatcaatgct tgccatggat gacaagaaat acacttttga agtggacact cgcgcaaaca
     2041 aaactttagt aaaacaagcg gtagaagctg cttttgacgt taaagtcaaa aacgtgaaca
     2101 tcgtgaacgt acgtgcgaaa ttcaaacgta tgggcaaata tgcaggatat acaaaaaaac
     2161 gtcgcaaagc gatcgttaca ttgactgaag attcaaaaga aatccaatta ttcgaagctg
     2221 ctgaataagc actaatagat aactcattaa attaggaggg aaaaacgtgg cgattaaaaa
     2281 gtacaaacct acctcaaatg gtcgtcgtaa catgacaggt tctgattttg ctgaaatcac
     2341 aacatcaaca cctgaaaaat cattgttaca accattgaaa aaccatgctg gacgtaacaa
     2401 caacggccgc atcactgtac gtcatcaagg tggcggacac aaacgccaat atcgcgtgat
     2461 cgacttcaaa cgtaacaaag ataacgttgt tgcaactgtt caaacgatcg aatatgatcc
     2521 aaaccgttca gctaacatcg cgttagtaca ctatgaagat ggagtgaaag cttacatctt
     2581 agcacctaaa ggactagaag tgggtatgaa acttgtttca ggtccagaag cagatatcaa
     2641 agtcggaaat gctcttccat tagaaaacat tccagttggt actgttatcc acaacattga
     2701 aatgaaacct ggtaaaggtg gacaattgat ccgttctgct ggtacaagtg ctcaagtact
     2761 tggtaaagaa ggcaaatacg tattgatccg cttgaactct ggtgaagttc gtatgatctt
     2821 agctgcatgc cgtgctacaa tcggttctgt aggtaacgaa caacacgaat taatcaacat
     2881 cggtaaagct ggacgctctc gttggatgcg taaacgccca actgtacgtg gtagcgtaat
     2941 gaaccctaat gatcacccac acggtggtgg tgaaggtaaa gctcctatcg gacgtaaagc
     3001 accagtttct ccttggggtc aaccagctct tggttacaaa actcgtaaca aaaaagctaa
     3061 atcagacaaa cttattgttc gtcgtcgcaa aaataaataa ttactttgac catgtgtcgc
     3121 acattttgag aggaggttca ccatgggtcg tagtttaaag aaaggacctt ttgtcgatga
     3181 gcatttaatg aaaaaagttg aagctcaagc aggggttgaa aagaaaaaag ttatcaaaac
     3241 ttggtcacgc cgttcaacag ttttcccaag ttttgttgga tataccatcg cagtttatga
     3301 tggacgtaaa catgtaccag tttacatcca agaagacatg gtaggacaca aattaggtga
     3361 atttgcacct acaagaactt atcgtggaca tgctgctgac gataagaaaa cgaaacgcta
     3421 atttgagggg aggataaatc caaatggcag aacaaatcac atcagctaaa gcaactgcaa
     3481 aaacagttcg cacttcacct cgaaaagccc gtttagtaat tgaccttatc agaggtaaaa
     3541 gtgtcgcaga tgcaatttca atcttgaaat tcacaccaaa caaatctgct ggaatcattg
     3601 aaaaagtatt gatgtcagca gttgcgaatg cagaaaataa ctttgactta gatgttgaaa
     3661 acttggtagt aagcgaagct tttgttaacg aaggacctac aatgaaacgt ttccgtcctc
     3721 gtgcaaaagg atcagcttct ccaatcaaca aacgtacaag tcatatcacg gtagtcgtat
     3781 cagaaaaata aggagggata atcagtgggt caaaaagtac atccaattgg aatgcgtgta
     3841 ggcatcatcc gcgactggga tgctaaatgg tatgctgaaa aagagtatgc tgagttctta
     3901 cacgaagatt taagaatccg taaatttatt tcaactaaac ttgctgatgc tgctgtatca
     3961 acagttgaaa tcgaacgcgc aacaggtcgc gtaaacattt caattcacac agctaaacca
     4021 ggtatggtta ttggtaaagg cggttctgaa gtcgaaagcc taagaaaaga attaaacaaa
     4081 ttaactggta aaagaatcca tatcaatatc gtggaaatca aaaaaccaga tctagatgca
     4141 aaattagtag gtgaaggaat cgcacgtcaa ttagaaaacc gtgttgcttt ccgtcgcgct
     4201 caaaaacaag ccatccaacg ctcaatgcgt gctggcgctc aagggatcaa aactcaagta
     4261 tctggtcgtc taaacggagc agatatcgct cgttcagaag gatactcaga aggaactgtt
     4321 cctcttcata ctttacgtgc ggacattgat tacgcatggg aagaagcaga tacaacatac
     4381 ggaaaactag gagttaaagt gtggatctat cgtggagaaa ttcttccaac taaaaaaaac
     4441 actgagaaag gagggaaata atcatgttag tacctaaacg tgtaaaacac cgtcgtgaat
     4501 tccgcggaaa aatgcgcggg gaagctaaag gcggaaaaga agtagcattc ggtgaatacg
     4561 gtttgcaagc tgttgattca cattggatca caaaccgcca aatcgaagct gctcgtatcg
     4621 caatgactcg ttacatgaaa cgtggtggga aagtatggat taaaattttc cctcacaaat
     4681 cttatactgc caaagcaatt ggggtacgta tgggttctgg taaaggggca cctgaaggat
     4741 gggttgcacc agtaaaacgt ggtaaaatca tgtttgaaat cgcaggcgtt cctgaagaag
     4801 tagctcgtga agcgttacgt ctagcttctc acaaattacc aatgaaaact aagatcgtaa
     4861 aacgtgagga aatgggtggt gaatcgaatg aaggttaaag aaatcagaga attaaccact
     4921 gccgaaatgc ttgatcaaga aaaacaatta aaagaagaat tgtttaatct tagattccaa
     4981 ttagcaacag gtcaattaga aaacactgca cgtattaaag aagtacgtaa atcgattgca
     5041 cgcatcaaaa ctgtgttgcg tgaacaagct aagtaattat ggaaggaggc cattaagcgt
     5101 atgactgaag gaagaaatca acgcaaagtt tatcaaggtc gcgtggtttc agacaaaatg
     5161 gataaaacaa ttaccgttgc tgtcgaaaca aagaaaaacc accctatcta tggcaaacgc
     5221 atgaactatt ctaagaaata caaagtgcat gatgaaaaca acacagcaaa agttggcgac
     5281 atcgtgagaa ttatggaaac tcgtccatta tcagctacaa aacgtttccg tttgttggaa
     5341 gtagtcgaag aagcagttat tatctaataa cacgagattt tcctatatag attgaatgca
     5401 aaatctgaaa ggaggataca caacgtgatc caagcagaaa gtcgtttaaa agtcgctgat
     5461 aactcaggcg cacgtgaaat tttgacgatc aaagtccttg gtggatctgg tcgtaaaact
     5521 gctaacatcg gtgatgtgat cgttgctacg gttaaacaag caacgccagg tggggttgtc
     5581 aaaaaaggtg acgtcgttaa agccgttatc gttcgaacaa aatcaggagc tcgtcgtaca
     5641 gacggttcat atattaaatt tgatgaaaat gctgcagtga ttattcgtga tgataaaagc
     5701 cctcgtggaa cacgtatctt tgggccagtt gcacgtgaac tacgtgaaaa caacttcatg
     5761 aagatcgttt cactagcacc agaagtttta taatcttgag acacttatca aaggaggtgc
     5821 gaaacagtta tgtttgttaa aaaaggcgat aaagttaaag ttatcactgg aaaagacaaa
     5881 aacaaagaag gcgttgtact agcagcgttc ccgaaaaaag ataaagttat tgtcgaaggt
     5941 gtaaatgtcg ttaaaaaaca ccaaaaacct tctcaagctg ctcctcaagg tggaattgtt
     6001 gagatggaag cgccaattca tgtttctaac gtgatggtgg ttgactctac tggtgtagct
     6061 ggccgagttg gctacaaaga agtagacggc aaaaaagtcc gtgtttctaa aaaaaccggt
     6121 gaagttttag ataaataaaa gtttaaggaa ggagggtctt actgaatgaa ccgcctaaaa
     6181 gaaaaatatc ttaaagaagt aactccatca ttgatggaaa aatttaacta tagctctgtt
     6241 atgcaaacac ctaaagttga aaaaatcgtc atcaacatgg gtgttggtga tgcggtatca
     6301 aacgctaaaa acttagataa agcagttgaa gaattaactt tgatcactgg tcaaaaacca
     6361 atgattacaa aagctaaaaa atcaatcgct ggattccgtt tacgtgaagg aatgccaatt
     6421 ggtgcaaaag ttacattgcg cggagaaaga atgtacgaat tcttagacaa attggtttct
     6481 gtttcactac cacgtgtacg tgacttccat ggtgtaagta aaaaagcctt tgacggtcgt
     6541 ggaaactata ctttaggtat taaagaacaa ttgatcttcc cagaagtaga ttatgattta
     6601 gtagataaag tacgtggtat ggacatcgtt attgtaacaa cagcaaactc tgatgaagaa
     6661 tctcgtgagt tgttgacaca attaggcatg ccattccaaa aataattaaa ggaggcgaac
     6721 tacgtggcta aaaaatcaat gattgctaaa aacaaacgtc ctgcaaaatt ttcaacacaa
     6781 gcttatactc gttgcgaacg ttgcggacgt ccacattcag tttatcgtaa gttccatctt
     6841 tgccgtattt gcttccgcga acttgcctat aaaggtcaaa ttcccggcgt gaagaaagct
     6901 agctggtaaa aagtaaattc gcataagaag gaggtaaaag atcaatggtc atgacagatc
     6961 caattgcaga ttttctaact cgtatccgta acgcaaacat ggttaaacat gagtctttag
     7021 aagtacctgc atcaaaaatt aaacgtgaca tcgctgaaat cttgaaacgt gaaggtttcg
     7081 ttcgtgatgt tgaatacatc gaagatgaca aacaaggcgt gatccgtgtt ttcctaaaat
     7141 ttggtaaaaa cggcgaacgt gttatcacaa acttaaaacg tatctctaaa ccaggtttgc
     7201 gtgcttatgt caaagctgac gaagtaccaa aagtattgaa cggtttaggt atcgcaatca
     7261 tttcgacttc tgaaggtgtt gtcactgata aagaagctcg tgaaaaaaat atcggcggcg
     7321 aagttatcgc ttacgtatgg taatcaagta gaatctacaa ggaggtgtct ttaagtgagc
     7381 cgtatcggaa ataaagtcgt tgttatccct gaaggagtaa cagttactca agacggaaac
     7441 aacgttacag ttaaaggacc aaaaggtgaa ttgactcgta ctttttcagc agatatcaaa
     7501 atgaatatcg aaggaaacga agtaactttc actcgtccaa acgacagtaa agaaatgaaa
     7561 acaattcatg gaacaactcg tgcgaacttc aacaacatgg ttgtaggtgt cagcgaaggt
     7621 ttccaaaaag cgttagaact aatcggggtt gggtaccgtg cacaattaca aggatcaaaa
     7681 cttgtattga acgttggtta ctcacatcca gtagaaatca caccaccagc tggagtaact
     7741 gttgaagtac cagctaacac acaagtaatt gttaaaggtg cgaacaaaga agaagttggc
     7801 gaattagctg caaacattcg tggaactcgt cctccagaac cttataaagg taaaggtatt
     7861 cgttatgttg gcgaatatgt acgccgtaaa gaaggtaaaa ctggtaaata atcgtttaga
     7921 aaaattgcat agaagccagc agcaggaaaa aggcatttgg ctgaagttta ccttcaaaaa
     7981 aatacatttt tacttgtgcc gcagcttcta tttttcgaaa cgtatttact gcttgaaata
     8041 gtagtacaat aaaaataaag aggtgacaat tgtgattaca aaaccagata agaacaaaac
     8101 acgtcaaaag agacatcgtc gtgtacgtaa caatatctct ggtactgctg agcgcccacg
     8161 cttgaacgtt tttcgttcta acaaaaacat ctacgctcaa attattgatg acgtagcggg
     8221 tgtaacgcta gcaagtgcct ctaccttgga taaagaaatt tcaggtggaa caaaaacaga
     8281 agcagcacaa gctgtcggta aactagttgc tgaacgtgca gctgaaaaag gcattaaagt
     8341 agtagtcttt gaccgtggtg gataccttta ccatggccgt gtgcaagctt tagctgaagc
     8401 tgctcgcgaa aatggactag aattttagaa gaaggaggaa taccattcat ggtttatatc
     8461 gatccaaaac atttggaatt agaagaccgc gttgttgcaa ttaaccgtgt aacaaaagtt
     8521 gttaaaggtg gacgtcgtct acgttttgct gctttagttg ttgtcggtga caaaaacgga
     8581 cacgtagggt ttggtacagg taaagctcaa gaagttcctg aagcaatccg taaagcaatt
     8641 gaagatgcga agaaaaactt agttgaagta cctatggttg gttcaacaat cccacacgaa
     8701 gtaatcggtg cttttagtgg tggtagaatc ctaatgaaac ctgctgtaga aggttctggg
     8761 gttgctgctg gtggaccagt tcgtgccgta ttagaattag ctggggtagc agatatcact
     8821 tctaaatctt taggctcaaa tacaccaatc aacgttgttc gcgcaacagt tgaaggtttg
     8881 aaacaattga aacgtgcaga agaagttgca gaacttcgcg gcaaatcagt tgaagaatta
     8941 atcggataag gaggacaaca ataatggctg aattaaaagt tactttaaaa cgcagtatta
     9001 tcggacgtcc tcaaaaccaa cgcgatacag ttaaagcgtt aggtcttaca aagattaaca
     9061 gttcagtggt taagccttct aatgaagcaa ttaaaggcat gatcaatact gtttctcacc
     9121 tagtggacgt tgaagaaatt taatttagaa aaatcataac tagaacatta aggaggtgcc
     9181 aaacctatga aacttcatga attaaaacct gctgaaggtt cacgccaagt acgtaaccgt
     9241 gttggtcgtg gtacttcatc tggtaacgga aaaactgccg gacgcggaca aaaaggacaa
     9301 aaagctcgtt caggcggcgg tgtgcgttta ggatttgaag ggggacaaac accattgttc
     9361 cgtcgtttac caaaacgagg attcacaaac gtgaaccgta aagactatgc agtcattaat
     9421 cttgatgtat tgaaccgctt tgaagacgga gctgaagtaa ctcctgttgc tttagttgaa
     9481 gctggaatcg tgaaaaacga aaaagctgga atcaaagtat tagctaatgg ggaactaaac
     9541 aagaaattaa ctgtgaaagc agcaaaattc tctaaagcag ctcaagaagc aattgaagcg
     9601 gctggtggat caatcgaggt gatctaatgt ttagactatt aaaagatgct tttaaagtaa
     9661 aagacattag atcgaaaatc ctatttactg tatttgtgtt gtttgtattt cgcctaggtg
     9721 cacatattac tgtaccaggc gtaaatgctg cttcattgca aaacctttct tctttgccat
     9781 ttttcaacat gttgaacttg gtaagtggta gtgcgatgca aaacttttcc attttctcaa
     9841 tgggggtttc accatacatt actgcttcta tcgtcattca gcttcttcaa atggacatcg
     9901 tgcctcgatt tgttgaatgg tcgaaacaag gggaagttgg tcgtaaaaaa ctgaatcaag
     9961 cgactcgata tctgacatta gtattggcat ttatccaatc tgtcggtatc actgctggat
    10021 tcaatcaata cgctcaatta ggtttcgtga ataatcctaa cgtagcaact tacgtaacga
    10081 tcggtttgat tctaacagcc ggtacgatgt ttgttacttg gctaggagaa cagatcacag
    10141 aaaaaggtat cggaaatggc gtatcaatga tcatttttgc aggtatcatt tctagattgc
    10201 ctgaaagtat cagagaaatt tatgaagatt actttataaa tattgatcct tcagacatat
    10261 ggaaatctgc tatttttgtg gccattttac tagtagcggt cttgttgatc gtgacattcg
    10321 taacattttt ccaacaagct gaacgcaaga tcccgatcca atatacgaaa cgcgtagctg
    10381 gcgcgcctac aagcagctat ctgccgttga aagtaaatgc agctggagtt attccagtta
    10441 tctttgctag ttcgtttatt gctacgccaa atgctgtttt acaagcattg agcggtagat
    10501 tcggttctga aaactggtac aaagtgatga tggagatctt taactataat acagcacctg
    10561 gtgctacgat ttatacgatt ctgatcgttg ccttcacttt cttctacgcc tttgttcaag
    10621 taaaccctga gaaattagcg gaaaacttgc aaaagcaagg aagttatata ccaagcgtac
    10681 gccctggtaa ggggactgaa gagtatgtct caagattact gatgcgttta agtacagttg
    10741 gtgcagtctt ccttggttta gtggcattgc ttccaatcat tgctcaaatg gtttgggatc
    10801 ttccacaatc aatcggatta ggcggtacaa gcttactgat cgttatcggt gtagctttag
    10861 aaactgcgaa acagttagaa ggcttaatgc tgaaacgtaa atatgttgga ttcattgagt
    10921 aagaaagcgt gttgaggttt cccctcaaca cacgttctca cacatttatt aggagggaaa
    10981 acaatgaacc tcattttaat ggggctgcca ggcgcaggta aaggaacaca agctgaaaaa
    11041 atcattgatg cttatggtat tccgcacatc tcaacaggag atatgttccg cgcagcgatg
    11101 caaaacgaaa cagctcttgg cctggaagca aagtcttata tggataaagg tgcattagtt
    11161 cctgatgaag taacaaatgg aatcgtgaaa gagcgactag ctgaaccaga tacagagaaa
    11221 ggcttcttat tagatggctt tccacgtacg ctagatcaag caaaagctct ggacgcaatg
    11281 ctaaaagatt tgaataaaaa aattgatgct gtcatcgata tccatgttgg tgaagaaatc
    11341 ttggtcgaac gtctagccgg ccgatttatc tgcagcaatt gtggagcaac ttaccataaa
    11401 attttcaacc ctacaaaagt tgaagacaca tgtgatcgtt gcggtgggca tgaattctat
    11461 caaagagaag atgataaacc tgagacggtt aaaaatcgtc tggctatcaa catcaaaaat
    11521 agtgaaccaa tcttggctta ttataaagaa caaggtttgc taaacactat tgatggtgct
    11581 cgtgaaatcg atgctgtatt tgcagatgta caaaaaatca ttgaaaaata gcacgattgc
    11641 ccgtagccga cttgcattat gcagttactc ttgtcaagtt caataaatta tgatagaata
    11701 tgtcagtccg acttctagag aatcctctag atgatccatt ttgagaattt tgaggtggga
    11761 actgcagttt ccgccatttt atcgtgtgaa aatgcgaaac aagtacaagg aggtactgtg
    11821 cgtggcaaaa gaagatatga ttgaagtcga aggtacagtc gtcgaaactt tgccgaatgc
    11881 aatgtttaaa gttgaactag aaaacggaca ccaagttctt gctactgttt caggtaaaat
    11941 tcgtatgcat tacatccgta tcttacctgg tgacaaagta acggttgaat tgtctccata
    12001 cgatcttaac cgtggccgta ttacttatcg ctttaaataa ttgtactccg taaaactaca
    12061 ggaggtatta tcatgaaagt aagaccatca gtaaaaccaa tgtgcgaaca ttgtaaagta
    12121 attcgtcgta aaggacgcgt tatggtgatt tgcccagcaa atccaaaaca taaacaacgt
    12181 caaggataat tggaggtgct ataaatatgg ctcgtattgc aggagtagat attcctcgtg
    12241 ataaacgtgt agtagtttca cttacttata tctatggtat tggtaacact actgctaaaa
    12301 agatcttagc tgatgctggt gtatctgaag atgttcgcgt tcgtgaatta acgaatgaac
    12361 aaacagatgc tatccgtgca gaagtagata aattaaaagt tgaaggggat cttcgtcgtg
    12421 aagtgaactt gaacatcaaa cgtttgatgg aaatcggttc ttaccgtgga atccgtcatc
    12481 gccgtggatt gcctgttcgt ggacaaaaca cgaaaaacaa tgcacgtact cgtaaaggcc
    12541 cagctcgcgc tatcgctggc aagaaaaaat aattcttaag tgaaggaggt taaaacttca
    12601 tggcagcaaa aaaagtgaat cgtaaacgcc gtgtgaagaa gaatatagaa gcaggtattg
    12661 cacatatcca ttctacattc aataacacta tcatcatgat cactgatact cacggaaatg
    12721 cattagcgtg gtcatcagct ggttcattag gtttcaaagg aagcaaaaaa tcaactcctt
    12781 ttgccgctca aatggcagca gaaactgcta caaaagcagc aatggaacac ggattaaaga
    12841 ctgttgaagt aacagttaaa ggacctggtt ctggacgtga agcagcaatt cgttcattac
    12901 aagctacagg tttagaagtg actgcaattc gtgacgtgac tccagttcct cataatggtg
    12961 cccgccctcc aaaacgccgt cgtgtttaat gagtgccgct cattttgagt tttatcagta
    13021 acgtcattga acaagacact tttcgttttg aaaggggtaa agataagaat gattgagttc
    13081 gaaaaaccaa gaatcgcaaa aattgatgaa gaaaaagatt atggcaagtt catcgttgaa
    13141 cctcttgaaa gagggtatgg gactacttta gggaattccc tacgtcgtat tttattatct
    13201 tctttgccag gtgctgccat cactaatatt caaatcgatg gtgtactgca tgaattctct
    13261 accatcaaag gtgttagaga agacgtcact caaatcatct tgaacattaa aggcttagcg
    13321 ctgaaaatgt atggccaaga agaaaaaacc cttgaaatcg atattaccgg acctgctaca
    13381 gtaactgctg gcgatattat cgttgatagt gatgtagaaa ttcttaacaa agatatgtac
    13441 atctgtagtg tctctgaagg agctacgttc catgctcgct taacagtgaa acctggacgt
    13501 ggttatgttc aagcagatga aaataaaaaa gaagatatgc caattggcgt tcttccagtc
    13561 gattcaatct acacacctgt tcgtcgtgtg aactatcaag ttgagaacac acgtgttgga
    13621 caccgtgaag attttgataa attgacgatg gagatttgga cagatggttc aattgaaccg
    13681 ttggaagcaa tgagtttgtc tgcgaaaatc atgactgaac atttggatat ttttgtgaac
    13741 ttaactgatg aagcaaaaaa tgctgaaatc atggtcgaaa aagaagaaac acaaaaagaa
    13801 aaaatgttgg aaatgacgat cgaagagtta gacttgtctg ttcgctctta caattgtttg
    13861 aaacgtgcag gaatcaatac tgtacaagaa cttacaaata aatctgagcc tgaaatgatc
    13921 aaagtccgta acttaggacg taaatcatta gaagaagtta aagcaaaatt gcacgactta
    13981 ggtttaggtt tacgtaaaga agattaaaac cttttacgaa ggagggaaac caacgtgagt
    14041 tatcgtaaac taggacgcac atctagccaa cgtaaagcga tgttgcgtga tttaacaact
    14101 gatttattaa tcaacgaacg tattgtgact actgaagctc gtgcgaaaga agttcgctca
    14161 actgcagaaa aaatgatcac tttaggtaaa cgcggagatt tgcatgcacg tcgtcaagct
    14221 gcggctttcg tacgtaatga agttgccagc gtgcgtgaag aaaatgagga catcgtcatt
    14281 gaatcagctt tacaaaaact gttcaacgat attgcacctc gttacgcaga acgtcaaggc
    14341 ggatacacac gcattttgaa gacagaacca agacgcggtg atggtgcacc aatggttgtc
    14401 ttagaacttg tctaaaatca cactaataac atcactttat agatgattct tttagagtgt
    14461 tatgatgttg gaagatattt ttccgagtct agctcagcgc tgctcctcaa gcgaatatcg
    14521 cttggggagt aagcactcat cataaagtgt ttttttatac cttcaattac ctgtcatgac
    14581 agatatgttt gaaggcataa aaaaacattt taagcgagaa ataattgaaa attttgtctt
    14641 tttttgatag tgttagtagg aactttagga tgaagaagga gtaatcatgg agccaattat
    14701 tgaattagaa aagataaatt ataaatacca gccagatgat cttcgccccg cattgaaaga
    14761 tgtctccttt acaattgata aaggtgagtg gatcgctatt attggacaca atggttcagg
    14821 aaaatcgaca cttgccaaaa cgatcaatgg attgcttctg ccagaatcag ggatcgtgaa
    14881 agtgggaaat caaatattgg atgaagaaaa tatttggacg atccgacaga tggttggaat
    14941 ggtttttcaa aatccagaca atcagtttgt tggttcgaca gtagaagatg acgttgcttt
    15001 tggtcttgaa aatcaaggga ttcccagaga agaaatgcta gtgcgtgtga aagatgcact
    15061 tgagaaagtt agaatggctg agtttgcttc acgcgagcct gctcgtttat caggagggca
    15121 aaaacaacgt gttgccattg ccggcgtggt tgccttacga cctgatatca ttattttaga
    15181 tgaagcaacc agtatgctag atccagaagg tcgtgaagaa gtgatctcta cgatcaagaa
    15241 gatcaaagaa gaaagtcaat tgaccgttat atctatcact catgatattg atgaagcagc
    15301 taatgccaat cggatactag tgatgagaca aggcgaactt gtccgtgaag gtacaccaaa
    15361 agaaattttt tctgctggtc ctgaactgat cgacttaggt ctagatctac catttcctga
    15421 aaaactgaag agcgcactga aagaacgtgg ggttgatgtg cccagtgaat atatgacaga
    15481 agaaaggatg gtggattggt tatggacatc cgttttgaac aagtagactt cacttatcag
    15541 ccgaatacac catttgaaca aagagcctta tttgacatca atatgacgat caaagaaaac
    15601 agttatactg cattagtagg gcataccgga agcgggaaat caaccttact tcagcatttg
    15661 aatgcgcttg ttaagccaac aagcggtaca gtccatattg gagaacgaga tattcagccg
    15721 gatacggata ataagaattt aaagcccatc cgaaaaaaag ttggcatcgt ctttcagttt
    15781 ccagaagcac agctattcga agaaacggta gcaaaagaca ttgctttcgg tcctaaaaac
    15841 tttggtgtca gcgaagaaga agcactagtc ctagcaaaag aaacattaga acaagttggg
    15901 ctggatgaaa gctatttgga acgttcgcca tttgaacttt caggagggca aatgcgtaga
    15961 gtagcaatcg ctggtgtgct tgccatgagg ccggaagtac ttgtattgga tgaaccaacg
    16021 gcaggacttg atccccaggg tagaaaagaa atgatggaga tgttttggcg tcttcataag
    16081 gaacagcaga tcactattgt attagttacg catttaatgg atgatgtagc caattttgca
    16141 gattatgtct atgtgttgga aaagggacga atcgttaata gcggggagcc gcaagaggtc
    16201 tttcaaaaca ttgaatggct aaaagaaaaa caactaggcg tacctactgc caccgagttt
    16261 gcagaagaat taatggctaa agggatgaac tttgctacct tgcctttgac agctgaagaa
    16321 ttagcagacg ctatcgtcca ttatgctgga ggaaatgccc atgatgaata aattgatatt
    16381 tggtcgttat atgcctggag attccttgat ccatcgattg gatccccgag ctaaattatt
    16441 agccagtttt tattttatcg gaatcatttt tttagcaaat aattgggaaa cgtacctttt
    16501 gttggcattt ttcacattac tttctgtctt cttgtcaaaa gtagatttga aattttttat
    16561 ccgaggcatt cgcccattga tctggctaat cctcttcact gtagctttgc aggtcttgtt
    16621 tacaagtggc ggagaaactt attggagttg gggtgttttc catatcactg aatttggtgt
    16681 gatgaacggc ctctttattt tctgtcgatt cgtgttgatc atttttatgt cgactctact
    16741 cacgcttact acaccgcctt tggaattatc ggatgcgatt gagtacttgc ttcgtccatt
    16801 gaaagccgta cggttcccag tccacgaaat ttcattgatg ctgtcgattg ctcttcgttt
    16861 tgtgcctaca ttgatggacg aaacagaaaa gatcatgaac gctcagcgcg ctcgcggtgt
    16921 tgattttggt gaaggaaacc tagttcagaa aatgaaagca gtcattcctt tgctgattcc
    16981 tttatttgtc agcagcttca accgtgcgga agatcttgcg actgctatgg aagcaagagg
    17041 ctacaaaggt ggggaaggaa gaacgaaata tcgtgtattg cattggcata atagagacac
    17101 gatggttatc attgcttttg tattattaac tgtgctactg atctttttga gaggataaac
    17161 gttcagtggg ccgctatcta ttcttttctt gctgatctgt ttgcaagaaa ggatgcgagc
    17221 agtgtatgac aagaaatatt agggtctgtg gcagactaag gtcacaggcc tctttctttt
    17281 atctgaaaag aaagaacgaa aaaattttat aaaaaaatca ttattccaga taacctttta
    17341 ggaacaaagg agagaaaaac atggtacgtt acaaagcaat cattgcatac gacggaacaa
    17401 attttaatgg attccaaaaa cagccaaatg ggcgtactgt tcaagaagaa gtagaaaaaa
    17461 cattacaaaa aatggcaaat ggaaaagaaa tcacggtctt tggttcggga aggacagatg
    17521 ccggagtaca tgcgattggg caagtaatcc attttgatta tccagaggag cgtccgttag
    17581 aacgaatgcg tttcgcgcta gatacccagt caccagagga tattgctgta agacaagtag
    17641 aaattgtttc agaagacttc cacgcaagat atttggtcaa agaaaagacg tatcaatttc
    17701 gagtggatat agggaaacca cgcagtcctt ttcgacgcca ttatgcaagt tattttcctt
    17761 accctcttga tttggaaaaa attcagcggg cgcttcctga tttattaggg actcacgatt
    17821 tcacctcttt ttgtgcatca ggaagctcga tagaggataa agtacgtacg atctacgagg
    17881 ctaagatgga ggtaaatgaa acaaaagacg aattgttgtt tacatttagg ggaaatggct
    17941 tcttgtataa gatgatacgg atcttagtag gaacgctttt gaaaattggc aatggccgat
    18001 tgcctgaaga tagtatccca gatattattg cgaaaaaaga tcgtaatgca gcaggaccta
    18061 cagcacatcc tgaaggactt tatttgtatg aagtgaaata tgaataagag gttgtgacag
    18121 aagtggtcag cttcaagaaa taagaaggaa tttacgaaaa ttacttctca aaattttgca
    18181 aaatttcggc ttattttcga aggagctact tctggaacac cgtgaatcag aggttgtgaa
    18241 aaagctgccc tgcatcaagt aataagaaca accaaacaaa aatagctcgt catatttttc
    18301 gtttggttgg gcttattacc gcagatgcaa gcttttgaac accgtttaag gaagaggttg
    18361 tgaaatcagc gttttgacct gagtattaga gaaacaaacg gaaaaatagt tcctcatatt
    18421 ttgccgtttg ttgagctaat accgaaggtc agctgattga acaccgttta agaaagaggt
    18481 cgtgagcctg tcatttaact ctgagtcaca agtaacaatt tcaaaaaaca gcttctcatc
    18541 tttgatgaat atcgtgactt gtggacgagg cgttgataag tgaatcatct ttttgttacc
    18601 tattttttct gtaaccaaaa aacgatggaa cgttgttttg attgccctta atgaggacag
    18661 tcggaacagg tttgtcattt ttatatttaa atggcacaaa tttttctaat tatttgtcat
    18721 ttagcgctga aaatttttgc tatttatcta aaaaagtgat aactacttaa gatgaagcag
    18781 aatatttacg ccatacggca tacaaccgcg aaaaatataa aaaaaagaaa acgattgaac
    18841 gcgtttttgc agtcgcaaag gaaaagcatg gaatgcgcta catcaagtat cgagacttgg
    18901 aaaacaatca ggtgtatacg ctgattgttt ttgctgcaat gaatctgaaa aagttgtcca
    18961 cttggcagtt ggaacatatg tcttcatatc ttttattata caaaaatagc aaaagcacca
    19021 gtctacactc ttagtaaaga ggaaactgat ccttttgtct acattcttaa agtacttaaa
    19081 ctaagtactt tattttactc tttaggattg atacagtagc atctatccct ttattaataa
    19141 tcttttattt ttttaaataa agatgttgtc tccatcttca ggcactaaaa ctttttcgga
    19201 aattcctttt tctttattga aagactttaa ttcttctctt gacagtcccc aatggtttac
    19261 agcttccata tgaacggcaa taattgtgga atctggtatt gctttatgca attcatataa
    19321 atcgtctttc cccataataa gagattctcc aactaaaaaa tgattatctc cagcatttgc
    19381 tactatgatt tctggattat actgatcaat agtttgttta acagcttcat accaaactgt
    19441 atctcctgta aagtatagtg tcttctcagt cgaatgtttg aaaacaatac cgcaaacatt
    19501 acctacataa ttcaagattt ctcctctgcc atgtttgcct tttgttttaa ccaattgtat
    19561 agattcaaaa actgtgtctt ctcgtagtac ctccacattt ttaaaaccat atagtcgaat
    19621 ttcattcgca tcattttcat cttgcacaaa taattttatt tctttaggta ggacctcttt
    19681 cgctatatcg tcccaatgat ctagatgtaa atgtgttaca ataacagcat caataccgtt
    19741 gataatatct tcgactggca ttggcaaatc aactagagga ttcatttgat cttttcttgg
    19801 tgatggaaaa ggacctttcg aaataaatgg tggaagtgtt cctttttccg ctaacattgg
    19861 atcaattaaa aattttttac ctccatagtc taagatagct gttgcattac gaatgtgatg
    19921 tatattcata aacgttgtct cctttttgtt gatatgaaat atttttagta taattcaatt
    19981 ccatacaata aataaaccga acaaaaaaag tcttttacta tttttactta atttatgaaa
    20041 ggttgtttac catgttagac caaacagata taaatatcat tgaggaacta tcaaaaaata
    20101 gtcgaatcac aatgaaagaa cttggagaaa aagtacattt gactggaccc gctgtctcgg
    20161 cgagagtgac aaaactagag gaaagtggag ttattgaaaa ttatacagta aaattaaata
    20221 tggaaaagct agggttttcg attcacgctt ttttaattat aattacgcaa aatttaaatc
    20281 ataaacccta tttatcattt attaaaaatc atgaaaaatt cttgattaga aattataaga
    20341 ttagtggagg gggctgttat cttttggaat gtagatttac ttcaaaaaaa tcaatgaaca
    20401 gctttttgga acagcttaat gaatatgcga attataaatt atctattgtt atagattaat
    20461 aataaaacta gcagatggta aaaatactaa tatgcgagac acacattcaa ccataactat
    20521 ataacttatc aatttaaaac taaactgatg gaaacaagta aggcaaaaac atttatattt
    20581 tgccctactt atttttttaa ttaaattcat tgatgattca tatttagaaa attctatgaa
    20641 ggccaaatga gtatcctgtc acttttttag ataaatagca aaaattttca gcgttaaatg
    20701 acaaataatt agaaatattt gtgccattta aatataaaaa tgacaccaag tatatcatga
    20761 acgcacaaaa agaccaacag ggtataccct gttggtctaa at
//
LOCUS       DANKEF010000046        20349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000046 DANKEF010000000
VERSION     DANKEF010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.046"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(322..852)
                     /locus_tag="PWZ48_001965"
     CDS             complement(322..852)
                     /locus_tag="PWZ48_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2261617.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
     gene            complement(1034..1690)
                     /locus_tag="PWZ48_001966"
     CDS             complement(1034..1690)
                     /locus_tag="PWZ48_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL2261618.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            complement(1817..2290)
                     /gene="dfrF"
                     /locus_tag="PWZ48_001967"
     CDS             complement(1817..2290)
                     /gene="dfrF"
                     /locus_tag="PWZ48_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HDL2261619.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            complement(2709..2927)
                     /locus_tag="PWZ48_001968"
                     /pseudo
     CDS             complement(2709..2927)
                     /locus_tag="PWZ48_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173391.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2958..4172)
                     /locus_tag="PWZ48_001969"
     CDS             complement(2958..4172)
                     /locus_tag="PWZ48_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HDL2261620.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(4513..4755)
                     /locus_tag="PWZ48_001970"
     CDS             complement(4513..4755)
                     /locus_tag="PWZ48_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2261621.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(4787..5344)
                     /locus_tag="PWZ48_001971"
     CDS             complement(4787..5344)
                     /locus_tag="PWZ48_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2261622.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(5357..5545)
                     /locus_tag="PWZ48_001972"
     CDS             complement(5357..5545)
                     /locus_tag="PWZ48_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2261623.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(5558..6124)
                     /gene="amaP"
                     /locus_tag="PWZ48_001973"
     CDS             complement(5558..6124)
                     /gene="amaP"
                     /locus_tag="PWZ48_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2261624.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            6541..6714
                     /locus_tag="PWZ48_001974"
     CDS             6541..6714
                     /locus_tag="PWZ48_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261625.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            7354..7827
                     /locus_tag="PWZ48_001975"
     CDS             7354..7827
                     /locus_tag="PWZ48_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HDL2261626.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            7836..8063
                     /locus_tag="PWZ48_001976"
     CDS             7836..8063
                     /locus_tag="PWZ48_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2261627.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            8699..9070
                     /locus_tag="PWZ48_001977"
     CDS             8699..9070
                     /locus_tag="PWZ48_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261628.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(9326..9568)
                     /locus_tag="PWZ48_001978"
     CDS             complement(9326..9568)
                     /locus_tag="PWZ48_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2261629.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(9600..10502)
                     /locus_tag="PWZ48_001979"
     CDS             complement(9600..10502)
                     /locus_tag="PWZ48_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HDL2261630.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(10515..10703)
                     /locus_tag="PWZ48_001980"
     CDS             complement(10515..10703)
                     /locus_tag="PWZ48_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HDL2261631.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(10717..11280)
                     /gene="amaP"
                     /locus_tag="PWZ48_001981"
     CDS             complement(10717..11280)
                     /gene="amaP"
                     /locus_tag="PWZ48_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HDL2261632.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(11318..12211)
                     /locus_tag="PWZ48_001982"
     CDS             complement(11318..12211)
                     /locus_tag="PWZ48_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2261633.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(12289..13212)
                     /locus_tag="PWZ48_001983"
     CDS             complement(12289..13212)
                     /locus_tag="PWZ48_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2261634.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(13261..13611)
                     /locus_tag="PWZ48_001984"
     CDS             complement(13261..13611)
                     /locus_tag="PWZ48_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2261635.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(13644..14546)
                     /locus_tag="PWZ48_001985"
     CDS             complement(13644..14546)
                     /locus_tag="PWZ48_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2261636.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(14539..15396)
                     /locus_tag="PWZ48_001986"
     CDS             complement(14539..15396)
                     /locus_tag="PWZ48_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HDL2261637.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            15730..16539
                     /locus_tag="PWZ48_001987"
     CDS             15730..16539
                     /locus_tag="PWZ48_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2261638.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            16579..17076
                     /locus_tag="PWZ48_001988"
     CDS             16579..17076
                     /locus_tag="PWZ48_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HDL2261639.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(17461..17534)
                     /locus_tag="PWZ48_001989"
     tRNA            complement(17461..17534)
                     /locus_tag="PWZ48_001989"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17498..17500),aa:Ile,seq:cat)
     gene            17721..18044
                     /locus_tag="PWZ48_001990"
     CDS             17721..18044
                     /locus_tag="PWZ48_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261640.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            18208..18462
                     /locus_tag="PWZ48_001991"
     CDS             18208..18462
                     /locus_tag="PWZ48_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261641.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            18532..18777
                     /locus_tag="PWZ48_001992"
     CDS             18532..18777
                     /locus_tag="PWZ48_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2261642.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            18853..19218
                     /locus_tag="PWZ48_001993"
     CDS             18853..19218
                     /locus_tag="PWZ48_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261643.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(19279..19842)
                     /locus_tag="PWZ48_001994"
     CDS             complement(19279..19842)
                     /locus_tag="PWZ48_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261644.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
BASE COUNT         5991 a         3918 c         3258 g         7182 t
ORIGIN      
        1 caatttctta ttttttgaaa tcaactgtct ttctcatatt tactattcaa ttttcatagt
       61 gatataggta caatttatat cactactgta aaaatggggt tttgccttat aaatacagcg
      121 ttttccgctt gttagtgtat agtgtttctt gttttttgtg ccccccttgt tagataacgg
      181 gggctttgaa cggctcgcaa gctcgccgaa ccgccgaagc gaaccgcccc cttgcaagca
      241 tggcggaaaa atcttaagga ttttcccgcc aacgtggtcg gatcgctccg tcggtttaac
      301 agcttttctg tttctctcta gttatctacg catataaatc aaatcggtta ttccgttata
      361 agtctgtgtt ttacaaagcc ttaactttat ttccctgtct atgttgccca aaagacgaac
      421 cccatttcct aacagagtag gaataacaga aatgtaatat ttatcaatca tatttttgct
      481 taccaactgc tgaacaagat ttgctccgcc acaaatccaa atatcctttc ctgcttcttg
      541 ttttagccgt tccaataata aaacaggatt ttcacttgta aaatgaattt gttgggaaga
      601 attgcactct ttatgtgtta atacataagt tgtaaattta ttatataccc attcttgcgg
      661 agaaagttca gtaacaacct gatgagaagt attccacccc attaagacag tatctatatc
      721 tttggtaaat tctgtataag tgtcgatgtt ttcattgtca tttccatgac catttagcca
      781 agctactcca ccattaccgt ccgcaatata tccgtcaagg ctcatggcaa taaataaaac
      841 tacttttctc attttttctc ttttataaat acaaacagga atttatttct cttttttact
      901 cataagggaa caactgattg ctacaaataa acccattaaa atccatggaa atgctgctct
      961 tataaaatca ctaaccatac tttattcctc cttaaattcc aatttcctta gttctacaaa
     1021 ctgaaatttt tgcctatgaa tgtatcttgt tctttatacg caaacaggct tctgtgtgca
     1081 taatccaatg tcttgaaaac ggcatttgaa ttagtccaca aatatcttta ttacaccaat
     1141 aatcaatcag ccaaattgct ttttcattgt cgtttactac attcaacgat tctaagtatc
     1201 cttttctttc tttcggtatt tttcttttca attcatcata agataaccgt tcaagcattt
     1261 tctcagtgct ttcccatact tcgaaaatat ataaatataa ttcttccaga ttaagctgtt
     1321 ttgaaaagtc tgcaatctgc tgtttcatga gttcatttcc tgttgtaatg ataggtgaat
     1381 tgatacgctc ttgataattg cctgcaaaaa atacttgttc atcttcattt atcactgtat
     1441 gcacaactat atcttcaatg cggaaaatat gccaaataga ataagcaatc gtcttactat
     1501 gataaccatc agcgtttatg aaggggattg agttaaaatc ttctctgcat aattcttctt
     1561 tgaaagatac taaggtttgt attaactgac ttcgtaaagt aagtaaagta tcaatacccc
     1621 tcttataagt atctttcttt tttatttgtg cctgcattgt tttattaagt tcagaccatt
     1681 ccttattcat attcaatcct ctctcaaatt ctgattttat cgccccatac aaactggaat
     1741 ttgtcttaca acaatttata tatgcataaa aagcaatata tttatgaaaa caccctaaat
     1801 ccaaaattta ctcaattcat tttttcctta cataaaatgt tctcgtatat ttcacttcct
     1861 caccaagtgt ttcccctatc aaaacttcaa aatcattgat atcaaattct ggaaagaatg
     1921 tatctccatc ttcaatattt aaatctactt ctgtgatata cattttatct actatttgca
     1981 aagcttcctt aaatagtcca tatccaccag atatgtatac atctcgtcct ttagccaata
     2041 ataatgcatc ttctaatgat ttaactgaaa ctaaattatc tccttgatac tctgttgtgg
     2101 tggaaacaac aatattcatt ctattaggca acggatgacc gatttcttca taagactttc
     2161 gccccataat aaccacatta cccgttgtta actctctaaa ttgcttttgt tctcccttta
     2221 ttttccatgg tatattacca ttcttgccta taacattatt ctttgacctc gcaacaatca
     2281 aacctatcat ctatttcatc ctccataata tgatgtcgta cctgtgattc catcaagtgc
     2341 accacatctt aacaaactca aaattatctc gaactaacca gctcgacaaa ttcctttttt
     2401 gtcttcatct gcattatatc atattgttat aagcattgcc atttcttttc tgcttttgaa
     2461 aagttactta tcagcctatg ttttgtaaag ggtgatattc agcatttctc acgcaacaag
     2521 cccttgacaa accatagact aataagtttt aaggtaatca agcagaaatg aaatagtctg
     2581 ctgtttcacg gcaacaaaaa agagatacca acacacttat gtagtatctc ttagtctagt
     2641 agcaatattc ttttgtttgc aagcaggtaa acatgggctg tttcttaata tctttttgtt
     2701 tgggaaacac ctttttccca tcctttcacc gttgccatct ctgctgcgca caatgccaca
     2761 gggataaacc atagcaaacc gcctaataac aagaaaaata ctaagctgaa gcctgaggtc
     2821 gcgaatgtgg gatattcata taatgaaacg accattgaga tggtcatagc caaaaagcca
     2881 aagaacgtta atttttttgc aggaattttc gttttgcttt gttgcatgaa tcttcctctt
     2941 ttctatttga atttgtttca attgagtttg ttgccaaaga agaaaaagtc caatccttct
     3001 tcttgttctt cttttgtagt agtgatcgtt gtaaagtgca tcgccgattt taaaatcact
     3061 gttgtcagca cataggcata cacggcagta atgatgacac caatcacttg aatccctaag
     3121 aagtaccagc tgcttggatt cgttagtaaa tggctgttag atgcaaagat ccctatcaga
     3181 atcgtacctg tgaatccgcc catgccatga acaccccaca catcaagtgc atcatcccat
     3241 tgtttctttt tacgaaaatc aacagcagca tgacagacga tacccgcaat gatcccaatc
     3301 agcatggcac tttttgcttc tacataaccg gcacaaggcg taatggctgc caatccagca
     3361 acggatcctg ttagtacgtc gacaaaatca aagccatttc cttttacttt tgcaatgatc
     3421 agccaagtaa tcatcgcaaa tgcaagagcg atgaatgttg aaccaaatgc attgactgct
     3481 tgttcatctg ctcgaagtgc accaccggaa ttaaaaccga accagccaaa ccaaagcagt
     3541 cctgtaccga tagcagctgc cataagactg ctatgctgac ttctttctcc taaaggaatc
     3601 tttctttgac caagtgtgag gatacatgct aaagagccga atccagctgt cgtgtggatg
     3661 actgctccac cagcgaaatc tctaaatccc atctgatcca aaaatccgcc accccatatc
     3721 cagtggcaaa cagggaaata aatcagcaga ttccaaaaaa taacaagtat aatatagcct
     3781 tttaaattca atctccctgc aaaagctcct gtcatcaaag gaacggtaat cacgataaac
     3841 atcagttggt aaagaaagaa cattaaaaat ggaatcgttg caccatgaaa catgttgggg
     3901 aagcttgtaa catgtctcat caaaaagaaa tctgccgggt ttccaataac accgccgata
     3961 tcccgtccaa aggccaatcc gaatccacca aatacccaca taatcgttgt tatcccaacg
     4021 gagagaaaag attgagacat gatcgttagt atattattct tccgtgctaa tccgccgtaa
     4081 aaaaaagcta gaccaggagt catcaggcaa accattgctg tacataaaat catgaatgct
     4141 gtatctcctg tattgatagg taatgtattc atttttttcg cctcctttct acgattacga
     4201 gtataaaaga aggtgtagtt gtttttttct tctgtttttt tgaattaata cttgcattta
     4261 taaaagacag acaattaata aaagcttgat gtgttgtgct ctttttcgtt tttcacacaa
     4321 ataaagacaa aaaataaacg gtcttctttt tatagaaaac cgtttatttt cgtctttttt
     4381 tatttttaaa gatatttttt cataaggaat aaagaatact tcatataaaa tatataaaaa
     4441 agaaccgcag cgagtgtcgc tacggttctc aaatttgttc ttcatttcaa cataagtttt
     4501 tcttcaattt gcttataatt tacgtgcaaa gaatgaaacg actaatacta aaatgattgc
     4561 accgatcaat gatggaatga tagccattcc tgcaattgtt ggtccccaag ttcctagtaa
     4621 agcttgtcca attgctgaac ctactaaacc agcaatgatg tttgcgatga tccccattga
     4681 tgatccttta ttcgtgattg ctcctgcaat tgctccaatg ataccgccga cgattaatgc
     4741 ccaaataaat cccattttaa attcctccta actgacttct tacttcttat tgtacacgtg
     4801 gttcgttatt ttctttgact ttttgtgcac ctttgcctac agcagatttt gctttatctg
     4861 tttggcgtga agcaaattgt cctgtggatt ttgctgcttc tgttactttg tcttgtactg
     4921 tttcttgatc tttttggtat tcttcttgtg ttttgatgtc tgcaacattc acattcactt
     4981 cgataacatc taaatgtgtc atattgttta cttcagtact gatcaattct ttgatttggt
     5041 tgtaaatatc ttcgatgtct ttaccgtatt ctacgatcac atctagatcc acagctactt
     5101 gtttcttacc tacttctgta ttgatacctg ctgttacgtt atctgtgttg actaattttt
     5161 cagccatatt tgagaaaaat ccgccatcta atgtcaatag accatctaca tctttcaatg
     5221 cataaccgat gattttttgg atgactttgt cttcataagt caattcacca ttcatcatgc
     5281 ggttttctcc tttttcattt gccaaaactg atccatttgt tgtattcatg tttttgtttt
     5341 ccatctttgt ttcctcctat gatttaaaaa attgatcaac tattcctgac tgtttcaaat
     5401 aaaaaccgat tcctgcgcca attgctgtca tgattatgat caacaatgtc ttgaaaaatc
     5461 caatcgttaa ccataaaaca gctagcagta gtccaagacc cccaaagata atagggagct
     5521 tgtatgctga aaatagttct ttcattattt ggccctccta ttccactcga gagtgcttgt
     5581 agtctcttgt atcttcttgt tcttcataac cgctgtatgt gactgccact ttgaccgttt
     5641 cttttgttcc gagtacttta gttacttctt cttcgatatc ttgcatcaat acacctgttt
     5701 tccctactaa tgaagaagtt cgtttcaagt ctccgttgac atggatatgg attcgatttt
     5761 tcgttgaacg tatccgtact ttaggtgaat gaatgaactc ttcttcgtgt aaatgggaac
     5821 gtaccaaccc ttcgatcgct tttttgtcga ttgttagttt gccatcttcc cgtttcataa
     5881 cgaaagttcc tcttgacttt ggatagaaaa gaacgactag caagatgata atagcaagaa
     5941 cggcaaatac tacagctgcc cagaaaatat attgttgcat gtagtaattc gttaaagtaa
     6001 agaagcgaaa tcgttccaat tgttcaggca taatatacgg cgcattgatc aacgcaatcg
     6061 ctagaaatac tgaaagtagc agtaaactca tgataacacc gattattttt aaccccttat
     6121 tcattttcca cctcctataa acttattttt accctgtaaa taactctacc gacaatcaca
     6181 gtatagatag cgaacagtta gcatagcatc agtaaataaa gcgctaccaa acattgctgg
     6241 atcataacgg cgttttattg ccagtctgat actgaaataa caattgctta cttattctct
     6301 atttactttt gattgctggc tatacttaaa ggatattatt caagattaaa aattgcaaag
     6361 attatgctca gttattatta caagattcta tttatttatt aaattttttc ggagaattaa
     6421 aaaatacaat aaataacttt agttacttta atgcgttctt actttatttt tatattatca
     6481 tttttcttat tcacttcttt ggtttatact attcttataa ataaaaacga ggtatgtcct
     6541 atgatgaaaa gattgtacta ttcattgatc ataacgattg gctatttgat tgtatcaaat
     6601 ctaggaaata tggtatttgg tatcagtaaa gaattcagtt ggactactac attatgggaa
     6661 tctctattct ttttcatctt cgtatttttg ttacaaaatt atcgaaaaaa ataaacatgt
     6721 gccatctatc tgttggcaca tgttttagga tttatggtac tttttattcg gcaatccatt
     6781 ttatttttct acttcttctt aatacctcta acacttttga tttataaact aatccaatgc
     6841 atctacttgc gcttattaag ctatttcagc tagatagtac caccccatgc gtcaatgctt
     6901 gattttcctt ttgtataaca ttgtataagc aataaaggac aaataagtgg tgattacacc
     6961 aaaccattga ataagaaaaa ttctgatacg taaataaaca tgaacttgaa aaaattaatg
     7021 aatcggttag ctactacgta gttgtctttt ttctcataca gttctgttaa cttacacttc
     7081 tcttcgctag ataataaaaa atcctccaac tgtattgtcc aatataatcc caggcatcta
     7141 aactagcatt atagaagaga cacacataga atctaaaata actggtttta aatgtttctt
     7201 acaatcaaaa aaagctgtta aatgcactcc acaccttcat actctatcat tttttataaa
     7261 cgatatattt ttattgtttt tcgataaaaa atagttgttt tacaataatt attctgctat
     7321 attttagcta tcaaagataa aagagggata aaaatgaaat ttaaaacatt gctattaaaa
     7381 tttgtcgttt taggtataag tgccgtattt attttgttcg gcgtactttt ttatactcag
     7441 atcgctacaa gcgaaaaagg attcaatttt gattggcaga ccacattatt tgtttctgtc
     7501 atttactttg cccttctcct agggatcgtt attgcttatt tcttattgaa actattgaaa
     7561 aaaatcgaag ataaccaagc tttttccact caaagcttaa acatactaaa aaaaatcgaa
     7621 cgaaccattt tgtttattgg tttcgccttt tttggcttac ttcctaaatt tatgaaagta
     7681 gcggatttag acgatgctcc aggtttgatg ttggtaggaa tcgctgtcgt attcattcct
     7741 tttgcagtat acacattaac aagcgtgctg gaaaaactgc ttgaacatgc gattttactt
     7801 aagacagatc atgatttgac agtgtgaggt gcgacatggc aattatcgtt aatttagatg
     7861 tagtattagc tcaaaaaaaa atgagtgtga ctgaattgag tgagaaagtg ggcattacga
     7921 tggcaaacat ctctattcta aaaaatggaa aagcaaaagc catccgtttt tccactctcg
     7981 aaaaaatctg ccaagttttg gagtgtcaac caggtgattt gttgacctat aaaaatgact
     8041 cgaatactat accagaagaa taaaataaag tagaacaaac agtaaataaa aaataatctg
     8101 aatgatagat gtgtgagact tttagaagtt atttacgtct attattcaga ttttttattt
     8161 cgattgaata ccgttttatc tataaataag atatgtactg actcatacaa agggggcctt
     8221 ttcttctcca tgtactatca cttttcctga tacaactggt ttcctgttac gttgtagttg
     8281 gatctgcttt tagtataata ctttattcat tccaccagac tgatagcagt ttgagcagtc
     8341 taaataatca tgtagtatta caggccattc tgtttcatac cttgaagtgc ttaaaacttc
     8401 agctaatggc ccattgtgtt aactcttact ccattctttt ttagtaagct cccatatata
     8461 ctgtaaagcc ttctatatat tatgtcatgt tgctttttaa catttactat gtagttttga
     8521 taaaatgatt tgaataacag tgatggacaa ttctttttta tttttaacat tattttgata
     8581 ataatgatta tttatttaaa aatacattag ataacatttg ttacgacacg aaaaagcttc
     8641 acaaacaaat atcgttattt taaaatgagg gcgaactcac acaattagga ggaatagtat
     8701 gccatttatt gtttactttt ttctttcatt actcgtgatc tatattccat taccaacatt
     8761 tttcctgctc gtcaatcgtc tagcagagca acatgatacg attgctgctc ttacgaaact
     8821 ttgtttactt ctatttgttt gtctagatta ctttacgagc tattattggc taaaacagac
     8881 aagagtaaaa aagagatatt atttcttcct tgtctttttc aatctctttg agtttctgat
     8941 ccattgcttt ttacttcatc agtatcatac gatccatttg tggatcatct gggttttcaa
     9001 agtggtcgta ttattttgta tgttcacctt tcctatctca agaaaattta gggaggcatt
     9061 gttttattaa agaagcttct ctgtatcaat aaagacacat ttaaggaaaa acttttaaaa
     9121 atctaattgg gaatttgccc gcataaataa gaggctgtga caaaagtctt gtcacagcct
     9181 cttattccgt ataaacagtg ttcaaaagct tgcatcttcg ataatatgcc caaccaaatg
     9241 aaaaatatga gaagctattt ttgtttggct gttcttatta cttgatgaag aacagctttt
     9301 tcacgacctc tttttaaaag tctttttaca gttttcgagc aaagaatgat acgattagta
     9361 ctaaaatgat ggcaccaatc aatgatggca cgattgccat tcctgcaata cttggtcccc
     9421 aagaacctaa taatgcttga ccaagtgctg atccaactaa acctgctacg acatttgcaa
     9481 taatccccat cgaagaacct ttattcgtga ttgctcctgc aattgctccg ataattcctc
     9541 cgacgataag tgaccaaata aatcccatgt ttcgttcctc ctaatgtttt acttccttat
     9601 tactgaaccc gtggttctgt attttctttt actttttgtg cgccgcttcc agctgctgat
     9661 tttactttat ctgtttgatt cgaagcaaat tcaccggttt ttttcgctgc gtcagttact
     9721 ttgtcttgca ccgtttcttg atctttttca aattcttctt ttgttttgat gtccactacg
     9781 ttagcgttga cttcgatcac ttctaaatgt gtcatatttt ttacttcttt gccgattact
     9841 tcttgcattt cttcaaagat tttttcgata tctttgccat attccacaat gacatccata
     9901 tcaactgcta cttgtttttt accgacttct gtttcgatcc cagccgttac attatctgta
     9961 ttcacaagtt ttcctgccac attagagaag aaaccaccgt ttgcagataa taaaccgtct
    10021 acttgttcaa ttgcgattcc gatgattttt tgcacaactt tatcttcata attcagctca
    10081 ccgcgcattg aatcttcctg ttcagaatca tgctttcctt ggttatttgt atgatcagaa
    10141 ccaagttttc cttgtccgtt tgtgtggtca gaactaagtt tttcttgtgc tttcgcatgg
    10201 tcagaaggat gtttttgctc ttccacaggt ttagaaggat cgattgcatc atgttgttct
    10261 ggaactttag attctggagt tcctggatga tgaggtgcat gcttttctgc atcatgtttc
    10321 ttctcttcta ctccttgttg aacgttaggg actactggat ctggatgtgc agatactggt
    10381 gctgttttgt tcttttcatc gttcacacca tttgattggt ttggtgtcgt tgggtctgga
    10441 tgtgcagaca ctggtttttg gttgttcatt ttattgttat tatttttgtt attgttattc
    10501 atggatgttt cctcctaaga tttaaaatag tgatctaaga ttttcgtttg tttcagataa
    10561 aaaccgatag cgacgccgat cgctgccatg atcaagatca ataaggtctt gaaaaagccc
    10621 acggtgatcc ataaaacggc taatatcaat ccaagtaccc caaagatgat agggagcttg
    10681 tatgtcgaaa atagttcttt catttttggt ccccccctat tctactcgtt tgcggtcatt
    10741 gcttcgttta gctggttctt caaagcctgt gtatttcacc gatactttca ctggctcttt
    10801 tgttcccaat atgtgttgta cttcattttc gatttctttc atcaatgttc ctgttttacc
    10861 aatgagtgct gaagttcgtt tcaactcccc ttttacattg acggcgatct tgtttttcgt
    10921 tgctttgaca tttacttttg gtgaatggat gaattcatct tcatgcaagt gagaacgaac
    10981 cattccttca attgcttttt tatccaacgt caactcccca tcaccatgtt tcagtagaaa
    11041 gtttccgatt gtttttggat aaaacaggaa aacaagtaac gtgataattg tcaaaatagc
    11101 aaatgcaacg gcaagccaaa atacatattg ttgcacgtat gaattagtga tggaaaagaa
    11161 tcgaaacttc tctagctgaa aaggcaatgg ataggttgca cttaatagta tcgcaaacaa
    11221 aagcacagat agcaaaagta atgttccgat cactccgatc gtttttacac ctttattcat
    11281 tttcgcacct cctcataggg gtgaaacgat caacttctta taagttgatc gcttctccac
    11341 cagttactcc gtaaatttgg gctgtaacat aacttgcttt attcgatgct aagaaaacat
    11401 aaactggtgc taattctcct ggctgacctg cacgttctag taaactttgc tgaccaaatt
    11461 cagggatcga atcagaaggt tgtccttcgt ctagttgtaa tggtgtccag attggaccag
    11521 gtgctacacc atttacacgg attcctttag gtgcaagctg ttttgctaaa ccgatcgtaa
    11581 agttagcgat tgaagctttt gttgctgcgt aatctaataa atgttcgctt ggattgaatg
    11641 cttgaacaga agttgttgtt acgattgctc ctcctgctgg gatatgcgga acagcttctt
    11701 tcactaaagc aaacatagcg atgatgttta tcataaatgt gtctttgact tgctcaagtg
    11761 gtaactcagc aatagactga caagaaattt gctgtcctgc gtttagtacc atcgtatcta
    11821 gcccgccaag ttcactcacg gctttatcta tgatttcttt aggtgcttgt tcgtcacgaa
    11881 gatcaaaagg tagtaagaca atcttacgtc ctgcatcttt cacgtatttt gctacttcct
    11941 ctgcatcttt ttcttctcct gggaagaatt gaagagcgat atctgcccct tctctagcaa
    12001 atgcaatcgc tgctgcacga ccgatccctg aatctccacc agtgattagt acacggcggt
    12061 tttctagttg attattgcct tcataggatt cttctccaca atcaggaaca ggaatcatat
    12121 tctgctgtaa agctggtgta tcctgatctt gtttaggaaa tttctccgta tggtacaagt
    12181 ttcgcgggtc gattaatttt ggttctgtca ttgctgtatt cctcctagtt tttaaattgt
    12241 attgccaaaa caaatttctt tgtttgatac ctgttttaaa tagattcttt aatttgtata
    12301 atctttccgg tataaatggt aacctaaaac tactgctgct atcaaagagc caaagactac
    12361 gagtataaag cctagagcag aacttttacc cggtaatcct cctgtattca ttccccaaat
    12421 ccctgcgaac aacgccggaa cggaaatgat taatgcagct gaatctagat atttcatgat
    12481 atgatttaga ttattgtcca tcatcccgtt gaatagatca ccaatactct ctaataaatc
    12541 tcgatagatc tcgatcattt tttctgtttg tcgctgtctc aaatggacat catagagtag
    12601 ctgttcatcg tctaaacgtt tgatgaaagt ttcagaattc cataattgat ccattgtttc
    12661 ccgttgatct cttaaggtat gatcgatata tacgatgtct ctttcaagat ctgcctgttt
    12721 atacagttct tcattttccg ttgttttacg agctgcttta tccaaagcgt caattcgctt
    12781 tttcatctct cttagttctt tcacgtaatg ggtgtaaata tctaataatg tatacgtgat
    12841 gatcttttca aacgagttta aatgatttcc atgctttgtc agcagccgat ccacaaattc
    12901 ggtttttcta tcgatacaag taatcaagag attatcggaa agtacaaaag atattggcgt
    12961 taattttttt tcgatacgat tatctgactg atctagatta atcactacca aagaaaatgg
    13021 atgctctagt ttcgtctcat aaaggcgctc catccgagaa acttcttccg gatattcggt
    13081 tcctacgaaa atatcatcag gaagctcgaa ttcttcgatt gcttgctcaa tctcttcggg
    13141 ctttgcacta tcaagtacga gccaattata ttttttttca tctgtttcta ttacattctt
    13201 ttcttcatcc acaaagtagt atctcaatgt atacctcctt tttggacttt ctcgaaacta
    13261 ttagctgaag aagcagttct ttaatttgat attataatcg agtactgcga tttcattttc
    13321 taatgaaaag ctgtcaagct gtttgttgaa gtccatgttc aatcgtttac cgaaataatt
    13381 ttgctctaat ttagatgtat agatgtccaa taatggatgc tcgattttag tcgcatattc
    13441 tccaaattcc gaaacaaaag gaatgatttg aaaaaagctg ccttttgcaa aagcaccttt
    13501 gtcccttaga aatggatcgg aatcattgat aagcgcgagt gcgatttttg tattttcccc
    13561 aaccttttca attaacgtct ctactgcatt tttagacatt gtaatttcca ttcctactcc
    13621 ccctcttcag ttgtggtctt ctattattta acaactacat ctgtttttgg ttgaactttt
    13681 gcatattttg gatatttagg gaatttagct acacaacgga aagccgtcat ccctgcatat
    13741 ttttcgtaat atttcttcgt tggcccttca aaatccttgc tccacggttt cacatgtcct
    13801 gaaaaatgga taatgtaagg attgttgcga gtttcttcgt attcacgttc acctgctgct
    13861 gttggatgtt tttttgcttt tgccatgata tagccttgtg cattccaacg cggatgaagt
    13921 ggtaaccaac ggtcatgtaa gatagcattt aatgcatctt ggtcatggaa tcgtaatttt
    13981 tctggattat gttcgatgaa atctaatact ttttgcgtaa tattttcatc taaccatttt
    14041 ttcacattga tcagcatcaa acctgagttg aaataacgca ttgatttagc tgaaattttc
    14101 attttttcca gtcgttgatg gaacccagcg tcttcaacag ctgctacgat tgaatcaccg
    14161 aagtcaagac gccataattt agagatatct tgtaaagcga tcatgtcgca atccatatat
    14221 aagatacgtt caacttctgt gccgcggaac aattccggaa tcgcaatacg ataataagct
    14281 gtttctggaa tacgatcgct gattaccaca tttgtaaaga attctttatt gattttcaag
    14341 aattccacat ctgagttcat tcgtgcgttt ttcactgaga agcgtaaaag ctgtttactt
    14401 tcaaaatcga tatcatcgtc aatcacatag aaccgaacaa atttagacgg gttgcaatta
    14461 tctaatactg agacaaataa tgcagctaaa tgtggtacga attttgtgtt acaacttgcg
    14521 actactgcta tttctttttt attcattgat ctcgcctact ttcgttagag tactgtgtgc
    14581 taattttttt aagtataaat tggtatacgg atgatctttc aacgtattcc aaggtttatc
    14641 atgtcctgta aagtgaacga tcgaaggttt ttcatttcct tctttatact gacgttcata
    14701 tttttcattt ggtgctggat gacgttcaaa aatcagcgaa gtctgcatgt tccattttgg
    14761 atgaagttgt tcccaatctt cataaagtac agcattcaaa gcatcttggt cgtgatagat
    14821 gattcgatcg ccattttcac tgaggtaatg gatagttttt tctgtaattt cttttttatt
    14881 ccattgatca atgtcaatca ccatcactcc tgaattgaag tacaagtcat cggaatcgat
    14941 tcctaatctt tccaaagctt tcacttggcc tggatcgata atagcgccga ttgtttttcc
    15001 atttaacggc tcatcatata gttttacgat atcgtctagt actaacacat ctgaatctaa
    15061 gtaaaggact ttcttataat cctctttagc taataagttt ggtaaagaaa ttcttaagta
    15121 agcggtagtt gtgatatggt cactgactag gaagtcttcg tagatatctt tttccactgt
    15181 caggaattgg atacttgcat tacttgaata tttatttacc gtttcttcca atccttcttt
    15241 actatattct gatagaccat cgtcaatcac ataaaatttt atgcgtcttg ttttattgca
    15301 attttctaat gcagttgcga tcatcacgct caagtaagga gcataatttt cgtctgaagc
    15361 agttaccaca ggaactacgc cgtatctctt ctccatcaaa atcactcctt tttctaatct
    15421 ttttctaacc tgttctacat ctagaatatt cctcatttgg ttttaaagca aaatatatgc
    15481 tttgttttca ttgaatattt taaaattttt ttatcacaaa acgcatcggt aagcgctaac
    15541 tattcgtttt tcatggcttt ttattcaaag gtattgacca aaaactacta tatatagcca
    15601 cctttttctt aagaatgttt tttgaaaaaa ttcataatga caaagcaagt tagtttcatt
    15661 tttttaaatt ctcatttaat cttaaggtaa caggaaaata gatatgtaaa aagaaagtag
    15721 gaagataaaa tgaaactagc agcgattgat ttagacggta ccttgcttga tcgaaatgga
    15781 aagataccag aagaaaatat ccaagcactt cggaaatttg atgaaaatgg cggaatcgta
    15841 accgtcgcta ccggaaggaa cagcatttct gctaaagata ttttttccga attaggaatc
    15901 ggtggctatt tgatttcttc caatggttca ctgattgctg aaatgaagga tggaaaaatg
    15961 gcgaaagttc tcagacgttc aaaaatagat agcgatacac tgagaaaagc cttcttctat
    16021 gctcaagaag cgaaaatatc catcattgct agccgagaaa cccaagatga tcagctgaca
    16081 ttcgatgaat caaatctagt caaagatgac ccttattatg aacatttcaa tttacagaac
    16141 cattcttttg atcaaatttt aaatatgttg gaagatcctt ctcttagcta tcttaaatta
    16201 gcattaacag ataagagtga agaaaaacta cagaagctac aaagagatct gcaagcagat
    16261 ggtatccaat cagtattctc tgatccgcat ttcttagaaa tcacgcctac aggtatcact
    16321 aaagctcatt cacttgtttt tctggcggat taccttggaa tcgaatcgag tgacatcatg
    16381 gcttttggcg atcaagaaaa cgacattgct atgttgaatt acagcgggtt gagtattgcg
    16441 atggggaatg cacagcgaca agtaaaagaa attgcaaacc aagtaacact gacgaatgaa
    16501 gaagctggtg ttgcttatat tttaaaaaac ttctattaaa aagaataaaa tgacgatatg
    16561 aatgtaggag gaaagaaaat gttagttatt attgatatgc aaaatcatat tcttgatcca
    16621 aacagcgaat tttatattcc agagtcagat caattggcag aacgtattgc tcaaagatta
    16681 gctaaagcac gccaaaataa tgaatatatt ctcttcacac gggatatacc agttgaactg
    16741 aaggacaaag aagaagaacg agaagatttg caattgatcc ctcttctttc tccacaagaa
    16801 aatgaacgag tgattaaaaa gtattacttt acgattccac ctgaaacttt agctgaaatc
    16861 caggaaactt tatttgaaag caaagaagaa caaaaagaga tcgaagtagt cgggattgaa
    16921 acaaacttgt gtgtactctc taatctgata ggcctgcaga gtgcttttcc tgaagcagac
    16981 ttttttgttg atccaatgtt agtctccagt agaaaacacg gagaatccgc tttagagctg
    17041 ttgaaagatt tcaacgtctc tatcgtcgaa tcataaaaat atctatcttc atttacgata
    17101 tttaacacac aataaaaaaa gcacgcagaa ctgttttagt cctacgtgct ttttctatca
    17161 aagtagattg gcttttttat gacacagagt gaaacggcag gctcacgacc tctttcttaa
    17221 atggtgttca atcagctgac cctcggtatt agcttaacaa acggcaaaat atgaggaact
    17281 atttttccgt ttgttaagct aatactcagg tcaaaacgct gatttcacaa cctcttcctt
    17341 aaacggtatt caaaagcttg catctgcagt aataagaaca gccaagtaaa aacataagaa
    17401 gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca caacctctta
    17461 tgtaccttgt aggactcgaa cctacgaccg gacggttatg agccgtctgc tctaaccaac
    17521 tgagctaaag gtacatcata aataaaattt atttttgtta cttttcaact atagcacgaa
    17581 tgacaaactc tcacaagttt caaaatataa gtaaaataat attgttattc ttatttcttt
    17641 tacttgtgtc ataactattt tgttacttta atttttattt gcttatttta gaaaaaatgt
    17701 tattctttta tttattctca atgttaggag tgattatttt gccatttatt gcgattttat
    17761 tcgatttagt tgcggcagca gcttattttc tccaattaaa ttatcaaacc tcaggaattc
    17821 tgtttattgg aatgattttt caaggtgtga ttaccctttt gcttcttgtc atgatgctca
    17881 gctataaagg aaaaagatat gcccgtattc aaacaaaagt atttgttaaa tatgtttcca
    17941 ttcgctatgg gatcatcatt ctttcctttt tagtgaatgc gatcgtcctc tttttatatg
    18001 tattgaatta cttagatatc aatccattga ttttttctcg atagataaga cacattcagc
    18061 ggaacagttc ctgcttatca gcagaaaact gttctgcttt ttcatttttc attgatctat
    18121 tcttctttta ttttcctagc atttcatttg tgatgtatgc aataacatgt tatcttttat
    18181 tatatgaatc gttgaggagt ccgttacatg attacgttgg tgagagtttt gttttgggtg
    18241 cctgcggtcg cactggtcgc aagtattgtt tatcttatga attggaacaa agaacggttt
    18301 tatttagcta ttttgactct tccggctatc tactttatgt ggaaagtttt taattacaac
    18361 tactttgaac ccgatagtgt ttttatagaa gaattatcag gactggtttt gtctttattg
    18421 atcgtcattc tttatttgat acgtttaaat aaaaaacatt gagtactatt gattgcatgt
    18481 gtagctttct atagtcatta taactattct actataatga aaggtgatta aatgagtatc
    18541 gaagaagcta ttttactgga tactttacgt tgtcgtctga atttcttaag aactggccga
    18601 tctttatctc aagtagctgt ttctagcgcg ttaagtattc ctttattcaa atatcgtaag
    18661 tacgaagaag gagaagaaat tccaagcatc attgaattaa tctatatcgc tgacttttac
    18721 gatatttccg ttgattacct aattggtcgc tcagaaacag caagcaaact gaagtgattt
    18781 tttccagcct tggtgctggt tttttcattt aacgtctaag gttaacaatc tatatataaa
    18841 ggagacattt ctatgcgatt atggcatgaa aaattgattt caaagcttcc tcgtcaacag
    18901 cttttaggac aacatcggga atgctgtgca ttaagaggaa atggctgggg aaagaaacat
    18961 gctactgtgg attatgtttt tcactattct ccttatagat tattccaata tcatcagctt
    19021 gtcatggatg aaatggaaaa acgtggttac agcccagcac cagagtggaa aaatcctatg
    19081 tatcgtggca aatcatgcga agcatttttt gatttaccta ctgtagagcg caacgtgccg
    19141 atttatccgg aacacgatga agtttatcta gcagaatgtt tagcaaattt ggagcaaaaa
    19201 ggaatttatc ttacctaata taactagaaa agccttctat ctcctcttac ttcattaaaa
    19261 agctttaacc atcttagatc aagctaaaat gtcgtaagtt ttagggttat cttcatcctc
    19321 acgtgcaaaa ctaaaagcgt actctagttt tggttcatcg ccaacttccc tagacataaa
    19381 tgaacatctc ctgacgtggt ttaaaagtac cgcatcttcg acgtctgtat acgatgcaca
    19441 gcacagattg cttcttttga tctttctttt gatttttctg ttgcttcttc tacaccttct
    19501 tcgattacta catcgatgct tacaattgct tctgcctaat attcccatgc atcgtcgatt
    19561 taagcctctg tagtcactgt gatcgtttcc acattttaac tcctctgtcg tggcttcagt
    19621 tgattttgtg atcagttcag ttgctaagcc agagtcagaa caacctgcca ttgctagcgt
    19681 taatattgat agtaaaaata gtttttttat cataaggttc cccccctgaa aatagactaa
    19741 aataataacg ctttcttttt ttgttttcag acgaaaatta ataatttcta aatattcttt
    19801 aaaaaagctt ataactatta tgcctttttt aatatataac acagttattt ttaaaaataa
    19861 gaatgctaaa tcactctaca ataacggcga aagcacaaaa aataaaaaaa ttattacgtt
    19921 attaaaaaaa cagatgaaag atcaggtttt attagatctc attctttcat cacttgaagg
    19981 gatttaatga actacttttt cctctttgtc gtttttatat attcgtattc cttcaaacag
    20041 taggatagcc tctttcaatg cttgatattt gaactgtccg ttacttaaaa atttagatgt
    20101 gccgccctct tcctagaaca tattcttttt tactttttca ttaattgaca gaccaatcga
    20161 acgaaactgt aaatttcgat aggataatga ttggtcaaac tcattcattt tcaagacatc
    20221 tcgattcata ttcaagatag gaatcttttt atcaacatca ctttattcgt attttcctct
    20281 accataaaag cttcacgctc cacaaaattg ttttatacac tactattcag cccattcaac
    20341 gaccaatga
//
LOCUS       DANKEF010000047        19656 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000047 DANKEF010000000
VERSION     DANKEF010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19656
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.047"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1011
                     /locus_tag="PWZ48_001995"
     CDS             <1..1011
                     /locus_tag="PWZ48_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HDL2261645.1"
                     /translation="YVADEDLLIYSIDESILKVTRSLNLFTTERTRSQRRKKLAQMIQ
                     EKIKEELGLIATVGIGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDF
                     WGIGSRMKKRLNQMGIMSIRDLANWNPYSIKSRLGVIGLQLYFHANGIDRTDIAIPPE
                     PTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILE
                     TRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRNVGITYSKLVYTDSLQLDLF
                     SDPQKQIDEANLDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIK
                     ND"
     gene            1004..1354
                     /locus_tag="PWZ48_001996"
     CDS             1004..1354
                     /locus_tag="PWZ48_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261646.1"
                     /translation="MINYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EISEVLNAAVIKNRLVCIQKEERNAEGYYPDDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            1351..1530
                     /locus_tag="PWZ48_001997"
     CDS             1351..1530
                     /locus_tag="PWZ48_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261647.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            1666..1959
                     /locus_tag="PWZ48_001998"
     CDS             1666..1959
                     /locus_tag="PWZ48_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2261648.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFIYDLSLASSCNMSDVYEKLLILENEYDQFNSNQKKKKKKHID"
     gene            2278..3120
                     /locus_tag="PWZ48_001999"
     CDS             2278..3120
                     /locus_tag="PWZ48_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2261649.1"
                     /translation="MNANSIVVGNFKGGTGKSTSTQMLGFVNAFYKKRKTLLIDLDPQ
                     ANASEVMSFTADNLGNHFYQENGFPKTIWNVLYDGSLDGAVLPLIENLDLIAGNIAMS
                     DYSDFMSAKFPNDKLAQFEYFANILAPLKEEYDEIFIDVPPSLGTIVQSAMYFAEYVA
                     IMLQTQPKSIRGAADYIDYMEFFTERYNTQLGIAGIIPFMLDTKTSTESYMYNEAKKL
                     YGDNLINTIVFRSARLIRYDETGITLETKKNGELKKVDANPQNTFISILDELNEHISW
                     FEEE"
     gene            3123..3677
                     /locus_tag="PWZ48_002000"
     CDS             3123..3677
                     /locus_tag="PWZ48_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261650.1"
                     /translation="MAERQSKLFPNKKARPRTNGMKDMEEFTKSLSISSADNSAPKVN
                     TTEKHSDDNKNLKNPNKSHKGKAGRKAEYKDPRLKKDLNTKISLSTKLRIQRLISRKF
                     DNKSEGDVIDIALDYLVSSFDRDDRDSLYKAYKEDMESMIPIIQEKNEKAKKQGKLTL
                     DITDEINQQTLKEQKEKWVSAKFE"
     gene            4325..5410
                     /locus_tag="PWZ48_002001"
     CDS             4325..5410
                     /locus_tag="PWZ48_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2261651.1"
                     /translation="MSTEFQSQKMIIKNWGYKATNVYIEKYYKFPSFLIQNSKYSKMK
                     DSSKIAYMLFKNEFRRALHEKWIDDDGLLYLEFTQADLMDKLNCYQGKVKTILNELET
                     YNLISVVKGEFNPKTKKNEKNKYYLLQPEISADDLFIQNSFDDDLSETIDKSTSSRNA
                     EIARREETAKTLDNTGNAEIARREKTAETLDNTGNAEIAQDYYINKSLDTKRHLKDTE
                     TDQLQNQVLLDNFVEIMKEDSINTFVPEKVLSLIKTFSNTYEEAQQTVTTIHNAKHKA
                     EELEKTVVVYEELESYGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEK
                     AIAAKLANEQRETFSELPTVSTENWLE"
     gene            6245..6577
                     /locus_tag="PWZ48_002002"
     CDS             6245..6577
                     /locus_tag="PWZ48_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2261652.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQNKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            7168..7497
                     /locus_tag="PWZ48_002003"
     CDS             7168..7497
                     /locus_tag="PWZ48_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261653.1"
                     /translation="MIQKFAKMSKTAWIEKLENDLSVMRLAPKKFKREIAEKEAILSV
                     LNAVDDKCPVKISFSDIATIIEGIYYGEVDEEISSTKDLLDFLDKNKDTFIVLTDYEE
                     VTTNEKL"
     gene            7484..7825
                     /locus_tag="PWZ48_002004"
     CDS             7484..7825
                     /locus_tag="PWZ48_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HDL2261654.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQKLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSV
                     EIIEFVADKEE"
     gene            7939..8178
                     /locus_tag="PWZ48_002005"
     CDS             7939..8178
                     /locus_tag="PWZ48_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261655.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            8245..8484
                     /locus_tag="PWZ48_002006"
     CDS             8245..8484
                     /locus_tag="PWZ48_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261656.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKQKHLKRLSVMQYINDMQIT
                     GKEKACLLGSMKNFEQLRRTYVKKSSNCQLLLEVS"
     gene            8570..9241
                     /locus_tag="PWZ48_002007"
     CDS             8570..9241
                     /locus_tag="PWZ48_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2261657.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGKVFKQNIEKKMPVD
                     QINEHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQAFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVLEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            9294..11270
                     /locus_tag="PWZ48_002008"
     CDS             9294..11270
                     /locus_tag="PWZ48_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2261658.1"
                     /translation="MAINRYVKKAGMLVGMLGIIASCFQGYQVHAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVINGTGAEHSFQIGDAVNYQGTIHIPTDIAGGTDGITVNGVKSETSPYSVFKWGDTA
                     GKGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDIQLTYNMFVNDSAAIANPLTNSVDFVYSNNPFNQEEHHEKTKADAVTYGAKFLKA
                     DGGLFGTGIKATPLEGAEFAVKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPTGYQKLTEDIAFKVNKNSFKEENRITVKNNQKAAI
                     PLTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            11283..12026
                     /locus_tag="PWZ48_002009"
     CDS             11283..12026
                     /locus_tag="PWZ48_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2261659.1"
                     /translation="MIIRKPPKRRVMHKLMIFFWLLSAVAVYLIPSVETNVAKFQQQQ
                     RILAIENLEKTDSSLDVAKIELGDPVGVLTIPSISLKLPIYDGTSDKILENGVGITEG
                     TGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIEEVQ
                     KDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEENRKQ
                     KLSWKRLLGSTVFLSVMIIGSLFVYKKKK"
     gene            12042..12815
                     /locus_tag="PWZ48_002010"
     CDS             12042..12815
                     /locus_tag="PWZ48_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261660.1"
                     /translation="MRNRKWLLVCAVALFSFTAYTQLTFADSQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGQKLEKQEGV
                     NGAKFIVYDITNILEEMSQDDNEKNQSVEIIESKLKDRAKKLSYDQLKKVAEGETKTI
                     DDQEGVIEFSIEVPANKKQAYYIVNESSPENISNSEDIVLLTPVSNEKGEFLTDVWLY
                     PKSEKSEPKEEIKKVVSTGVKKNFFENCWEFFAQLFFDN"
     gene            13116..15215
                     /locus_tag="PWZ48_002011"
     CDS             13116..15215
                     /locus_tag="PWZ48_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2261661.1"
                     /translation="MENKIHCTTGRVITLVLFLFLLLFARGTAVQALEKVDPSQIKEN
                     NNLVLYHDSDSLRNIEVTGINGFKILDEYSTKKQKVLAWPKVKDLKSTSKIVAHYQKV
                     GTYLGKEIDCDVTFSNFVAHAGVNQDGLAKTINGKKYYNYLLFDKNIYRGFFMQSINV
                     MNAKLEFKYAGTKTKVALSEPQPEIQSKMRSMRAAGIDLNDTFLSIYSLNGQYQQRVS
                     WYNDNNESVGERNIDMREFVGYEKMGSLPYFVTTDTACAEYKAPALSTNNLTSKVVGG
                     RAEKAYSSNDHFTDVVGADTYHRSGVTYTLQGASPTFMIGANTNSMMFSFDTALLWTP
                     QPSKPTKEVFNKANTEEAAHNIDKKVIPQGSDVYYHIHQKFDALTVNTMNKYKSFKIT
                     DTFDSKNFDMVSDGKNYDGALWYVEANGTWKKADTGGKLTVSGNTVTYTASDSFLANA
                     ANYGRDYILQLHMRAKNDAKGIAPNVATVTFNNDPNKGTQKTNTVENYFSLKPNKKVE
                     QDGKDVDGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDIASDHYKQFSIQDPVED
                     RLTYKEGSARVIDMSTGKDITSEGTLTYDSNSRTLKWEASADFLSKNLLDGREIQLIF
                     TAKTPLNEELDIDNQAKVSVESIEKATNVVTIGVKPNLPQIIVPKTGSNHLVLTVIGS
                     ILFLTLASLGYVVLKLK"
     gene            complement(15253..17310)
                     /locus_tag="PWZ48_002012"
     CDS             complement(15253..17310)
                     /locus_tag="PWZ48_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HDL2261662.1"
                     /translation="MKLFNPKTVLAIGTFAASLSLAGSVDAASTNVMYRLYNPNNHEH
                     FYTSSKKERDHLVKIKWGNYEGPAWEAPTSGGHLVYRLYNKGLRDHHYTASWDEVKWL
                     TKNYGWTYEGPAWRSAEKNNKPIYRLFLPGVKSGSHHYTASWKEVQWLTKDYGWKYEG
                     IGWYGADTSKPKVNKSALEALYTSVKGTKKGDYTDNTWNAFQTALNNAKKVLNDSKAT
                     QKQVDSAKNKLDSAYKGLKKKPVPTVNKAELQALYNRVKSTAKGDFTEESWNNFQTAL
                     SNAKKVLDDSKASQAQVNNAKNSLDAAFKGLQHKPETPKEYTVTINHIDNQTGQVIAT
                     DQAKVTSGSTYTAKAKAFKYSEGAKDNFSYKVIGNDTQSKTITGNTTITFNYNQVHQI
                     NLLCNNNYKNEGRVNILKEQIVNVTHGDSVTLTAPSIQGYVLDDRVEPTNSVTLNNVT
                     ESQNYIFNYTRKFNVTVNHVNADTNAVLSSESKTVYEGDSFATPWKNMTDQNYFLCRN
                     DDPTVTVDKNGQRSINNVDQNKTITFKYKKISLDELRNFMRQKELAWLNDYRQQNGVA
                     PMQFNDIVQQAADIRAKELQVSFSHYRPGGGTFQDLLESLGCYGAKGENISQTGLYID
                     DLLSDDGAIDAMVGWKNSPAHNANLLYNNQSILGLGHNFVVDSAGRIGVDNVFISANP
                     IFK"
     gene            complement(17424..17636)
                     /locus_tag="PWZ48_002013"
     CDS             complement(17424..17636)
                     /locus_tag="PWZ48_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261663.1"
                     /translation="MYADKKKIEWLLFDSGKSVRSISIESGVPLTTVSDLANKRSSID
                     NMRFINAMKLNKLAEEIVDINTSTNK"
     gene            17823..18290
                     /locus_tag="PWZ48_002014"
     CDS             17823..18290
                     /locus_tag="PWZ48_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261664.1"
                     /translation="MKKKQLWFVSMFAIFFIILSGCSSNKKSEAKEDNSLLQPGTVWK
                     EEVGGLIHNLKIIDDTTWEYSESVWHPDPVQITVKRQKDYEGLERYKIVDSAGIRKFI
                     NKPDSLFIVVPYEKDGVKKIVFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            18287..18529
                     /locus_tag="PWZ48_002015"
     CDS             18287..18529
                     /locus_tag="PWZ48_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2261665.1"
                     /translation="MIASLFIYPKGGIIVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            18547..18849
                     /locus_tag="PWZ48_002016"
     CDS             18547..18849
                     /locus_tag="PWZ48_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261666.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAVAA
                     VASIALGGIFFFFGRRGAENGKTHVGYVLLGVGVILAAASIVVSMFSMFGQTPQGL"
     gene            18921..>19656
                     /locus_tag="PWZ48_002017"
     CDS             18921..>19656
                     /locus_tag="PWZ48_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261667.1"
                     /translation="MFFTISGNAKLDKELIRKATKKVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGQVLEQENQSSVKLTLTLHDKEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKDTDETMSVEESYRNEKLLQVLEEELESDQRKVNQVSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETDEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNE"
BASE COUNT         7127 a         2899 c         3792 g         5838 t
ORIGIN      
        1 tatgtagcag acgaagatct attgatctat tcaatcgatg aatcaatctt aaaagtgacc
       61 agatcactga acctttttac gaccgaaaga acacgaagtc aacggagaaa aaaacttgct
      121 caaatgatcc aggagaagat taaagaagag ctaggtttga ttgctacggt aggcatcggc
      181 gataatcccc ttttggctaa attagcctta gacaatgaag caaagcataa tgaaggattt
      241 attgcggaat ggacgtacga aaacgttcct gagaaagtat ggaatattcc cgaaatgacg
      301 gacttttggg gcattggttc gcgaatgaaa aaaaggctca accaaatggg gattatgagt
      361 attagagatc ttgctaactg gaatccttac tcgatcaaaa gtcgtttagg tgtaattggc
      421 ttgcagcttt attttcatgc aaatggtatt gatcgaactg acatagcaat cccaccagaa
      481 ccgacaaagg aaaaatcata tggaaattca caagtgttac cacgagatta tactagaaga
      541 aatgaaatcg agttggtggt aaaagaaatg gcggaacaag tagcgatacg cattaggcag
      601 cataactgta aaacaggttg tgtccattta cacattggca cgtcgatctt agaaacgaga
      661 ccagggtttt cacaccagat gaagatccct ataacagata atacaaagga attgcagaat
      721 tattgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggaa cgttggaata
      781 acctatagca agcttgtgta caccgattcg cttcagctag atttatttag tgatccacaa
      841 aagcagattg atgaagcgaa cttggataaa attattgata aaatcagaca aaagtatggt
      901 tttacgtcga ttgtacatgc ttcttcaatg ttagaaggcg ctagaagcat tactagaagc
      961 acacttgttg gtggtcatgc gggcggaaat gggggaatta aaaatgatta attacaatga
     1021 tcgtggacgt ttaaagtggg cagggtttta tttatctgat catactgaag aaattgatgc
     1081 cgacaatgaa caacgaacca gtcaaaattt agcaaaagaa caaatgacga ctgaagagat
     1141 ttcggaagta ttgaacgctg cagtcataaa aaatagatta gtatgtattc aaaaagaaga
     1201 acgaaatgct gaaggatact atccagacga tataattggc aaaatattag gtagtgatga
     1261 gctggggatc tacgttggca aagacaaaat tcactacgac gaaatacgtc atatcgaatt
     1321 ttaccaggat aataaatgga gtcgattaga atgaacatta tccagcaata tgagctaaag
     1381 tatataacct ttgatcagct atcagaagag atttggggtt atggccaacg tttgataaat
     1441 gaggttggtg ttgagcgctt ctctttctat gtagaagctg cagcaggtta tcataatttt
     1501 aggttttata tcttcccgtt atatatataa ggtattttag ggattctata gagttatctc
     1561 tatgaatcct tttttgtttt tacaaatagg agtagatact agaataatgc gtgtgttaaa
     1621 ctattattgc aaacgcaata ataaaagaaa gaaggcttac gcaccatgtc tatcaataca
     1681 tttgtttact cgcacccgat caatgtttac attatcaaga atttaggaat cactgtagaa
     1741 caattttgtg agttatatgc atacccacaa ggaacggttg caagctggat tactcgacaa
     1801 cgtagaatca aatcactacc tgcttcattt atatatgatc tgagtcttgc ttctagttgc
     1861 aacatgtctg atgtctatga aaaattattg atactagaga atgagtacga tcaatttaat
     1921 tccaatcaaa aaaagaaaaa gaaaaaacac atagattaat attgaaacga aaaatagtat
     1981 ttattttaaa aaaagagaaa gtagaaaaag atatttttcc attatttgat taaagttact
     2041 tttaatcaaa actattgtaa aatagtactt gttttatgta tcttaaaatc aaaaaacttt
     2101 gattaaagat tcttcaatca aaaaaactta aaaacattga tataatagca tttaataaaa
     2161 ataagtatct cgtaatcaaa aaaataattt gattacgaga tacttattta catttacaac
     2221 agcaagttgt atacttaatt tgaataaaag atacttaatc aaaaggagcg taataatatg
     2281 aatgcaaatt caatagtagt tggtaatttt aaaggaggaa caggaaaatc tacttctact
     2341 caaatgctag gctttgtaaa tgcattttac aaaaaaagaa aaacattgct tatagatctt
     2401 gatccacaag caaatgcttc agaagttatg agtttcactg ctgacaactt agggaatcat
     2461 ttttatcaag aaaatgggtt tcctaaaact atatggaatg ttctttacga cggtagttta
     2521 gacggcgctg tccttcccct aattgaaaat ttagatttaa ttgccggtaa tattgctatg
     2581 agtgactact ctgattttat gagcgctaaa tttccaaacg ataaattagc tcaatttgag
     2641 tactttgcga acattctagc acctttaaaa gaagaatatg acgaaatttt cattgatgta
     2701 ccaccttcac tcggaactat tgtacagagt gcaatgtact ttgctgaata tgtagctatt
     2761 atgcttcaaa cacagccaaa atcaattaga ggggctgctg attatataga ttatatggaa
     2821 tttttcactg aaagatataa tacacaacta ggtattgctg gaattattcc atttatgtta
     2881 gacacaaaaa caagtacaga aagctatatg tacaatgaag ctaaaaagtt atatggcgat
     2941 aaccttatca acacaatcgt tttcagaagt gctagattga ttagatacga tgaaactggt
     3001 attacattag aaacaaagaa aaatggggag cttaaaaaag tagacgcaaa tccacagaat
     3061 actttcatat ctattttaga cgaactaaat gaacatatta gttggtttga ggaggaataa
     3121 agatggctga aagacaatca aaactatttc ctaacaagaa agcacgtcca agaacgaatg
     3181 gtatgaaaga tatggaagaa tttacaaaat ctttatctat atcttcagct gataattccg
     3241 ctcctaaagt taatacaaca gaaaaacatt ctgatgataa caagaatcta aaaaatccaa
     3301 ataaaagtca taaaggtaaa gctggcagaa aagctgaata caaagatcca agactaaaaa
     3361 aggatttgaa tacaaaaatc tctttaagta ccaaattaag aatccaacgc cttatttcaa
     3421 ggaaatttga taataaatct gaaggtgatg taattgatat tgctcttgat tatttggtat
     3481 cttcatttga tagagacgat agagattcat tgtataaagc atacaaggaa gacatggagt
     3541 caatgatacc aattattcaa gaaaaaaatg aaaaagctaa aaaacaaggt aaactaacac
     3601 tagatatcac cgatgaaata aatcaacaaa ctttaaaaga acaaaaagaa aaatgggtat
     3661 cagctaagtt tgaatagaag atacttaaaa caacataaac tattgattga agtatctggt
     3721 aatattgatt ttaagatacc tcaatcaata gttttttctt taaaataagc atttatgttt
     3781 gaataagaga tactaaaatc aaataaaagt atcttttaat caacaaacta gtttgaataa
     3841 agttactcat aatcaaatta taaatgattt ttagatacta caatcagata gaaaccctat
     3901 taaattaact ttcttaattg attaaaagat actaaaatga gattattttg attttagtgc
     3961 caaaaaacaa actaaaattt ttgattttag tatccttaaa tcaaattaaa tgtatattac
     4021 gagtcactat aatcaaaggt aatttattac gaatattgtc ttctcaataa aaagttcttg
     4081 attataagat ctttcaatca gatttatttt tgaataaggt gtcttccaat caaaagtaat
     4141 aactgattta agtatctctt attcaaaata tagttgtctg aaaataataa actgttaaac
     4201 ttattttgaa gcctatatta ttcctgattt gtatatcgaa attcattagc tatatacatt
     4261 taaagaaatc taattatatt tttgataata aaaattgtac ggatactaga aaagagggag
     4321 tttaatgtct acagaatttc aatctcaaaa aatgattatt aaaaattggg gatataaagc
     4381 aacaaatgtt tatattgaaa aatattataa atttcctagt tttttaattc agaattctaa
     4441 atattcaaaa atgaaagaca gctcaaaaat agcttatatg ctttttaaaa atgaatttcg
     4501 tagagctttg catgaaaaat ggatcgacga tgatggacta ttatatttgg aatttacaca
     4561 agccgatcta atggataaat taaattgcta tcaaggcaaa gtaaaaacca ttttaaacga
     4621 gttagaaaca tataatttga tttctgtcgt aaaaggagaa tttaacccaa aaacgaaaaa
     4681 aaatgaaaaa aataaatact atttacttca acctgaaata agtgccgatg atctttttat
     4741 tcaaaattct ttcgatgacg atttaagcga aactattgat aaatcaacct cttctcggaa
     4801 tgcggaaatc gcacgtcgag aggaaactgc taaaaccctt gataatactg gaaatgcgga
     4861 aatcgcacgt cgagaaaaaa cagctgaaac ccttgataac actggaaatg cggaaatcgc
     4921 acaagattat tatattaata aatctttaga cactaaaaga cacttaaaag acactgaaac
     4981 agaccaatta caaaatcaag ttttgctaga taacttcgtt gaaatcatga aagaagacag
     5041 cataaacaca tttgtaccag aaaaggtatt aagcttgata aaaacatttt ctaacactta
     5101 cgaagaagcc caacaaactg tcaccacaat acacaacgca aaacataaag ccgaggaact
     5161 tgagaagacg gtagttgtgt atgaagaact tgaatcttat ggaattgatg ctgacaaagg
     5221 attatatatg actttgctga aagcttatca aaaacaaaaa actgaaaaag taaacaattt
     5281 gcagaatcta attttcgttt atgtaaaaaa ttggtttgtc gaaaaagcta ttgcggcaaa
     5341 gcttgcaaac gaacaaagag aaacatttag cgaattacca acagttagta cagaaaattg
     5401 gctagagtag ttgataaaaa gcgatcacta taaaaatgat caattttaaa ttagaacttt
     5461 tttataattt taattttatt acaaatgtta caaataaata attagtattc cggagctttt
     5521 agattaaaag tttatgaaaa caatcacaaa cgtaggtttt aaagtcataa tcgttagata
     5581 ttttagttat ttttcattca aaaaaatagg gaactagtgt ttgcgtatgt gttttttttg
     5641 agttataata attcatgaag agaggtaatt cacctatttc cccaagctga aaagcctgat
     5701 attacttctt ttcaggcagt gctgctaata ttcatttaca tttttttgta tatttttagt
     5761 tgttttaaat ttaattgtct gctatgcttg tgatgaattg attttcgttc tgcacttctg
     5821 gacgataaat ttggacaatt gaaaatacaa aaaaaagaaa ggccctgtgt gactcggaag
     5881 gctaccaacc ttcgtcgacg tccacaggtg gcattaagaa cacccttaat gcgtttacct
     5941 ttcagtgtta cggtattacc cgtaacggat ttagtataac agatattcaa aagtgatacc
     6001 actttttttg gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag
     6061 cataatgtta taaaaatgtc cgtggggctc tttgccgtat gacgaacgct gaaattagag
     6121 ggaacgcatg tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc
     6181 tatttttgaa aaataacaag aaaaacttat taaaaatatt taaagtaagt gggtggatta
     6241 ttttatgtca tatacaggaa ttttgagtct cgaagatatt tgtcactacg gtaaacggtg
     6301 taccgctaca gagaaaatca cgaagaaact atcaactggc caaaataaaa ccgttgtgca
     6361 gtgtaagaaa tatataatcc agaaagacaa agtctcagaa gaaatgattt attacattgg
     6421 gaaacaaaag caaataattt taaaggatcc tatcccttta aaagagctat atccaaccat
     6481 caaacatgtt tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg
     6541 ttgtaccgca aaagggatgg ggcgtttgat cagctgaatt gatcattctc ataacttgta
     6601 ttttatcgtt tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagaatca
     6661 ttagaaaaat aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta
     6721 ggtttttacg attggtaacg taaaaaattg gtcaatcgag taaatgccct tgtttggata
     6781 gtggtgaaat gaatttgtgt aaataatatg cacagagtaa acaagaggga atttagaagt
     6841 tagacaagtt agaatagcgc ctagattaat catgagcagc tactaaggga tctaggcttt
     6901 gacgcaaact catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat
     6961 tgtgtcgtgt gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg
     7021 agcatttacc gctgtctact agggtttctc gtttcggacg ccataaacga gtcgatttat
     7081 tttatatatt tttgagtgaa cgaaaaacga taaagatatt cgttatgaag taaaactaat
     7141 ttagtagata gcaaaggtgg tagaggaatg attcaaaaat ttgcgaaaat gagtaaaact
     7201 gcatggattg aaaagcttga aaatgacttg agtgtcatgc gattagctcc aaagaaattc
     7261 aaacgagaga ttgcagaaaa agaagcaatt ttgtcggtat tgaatgcggt agacgataaa
     7321 tgtccagtaa aaattagttt ttcagatata gcaacaatta tcgaaggtat ttattatggt
     7381 gaagttgatg aagagatatc ttctacaaaa gatttattag attttttgga taaaaacaaa
     7441 gataccttta tagtactaac tgattatgag gaggttacaa caaatgaaaa attatgatcc
     7501 gaatatacga ttgggcacac atacgattaa ggtttctttc caacgttggg attacaaagg
     7561 atttgtgacg tttagaagag gcggaaattg taaaggatta gatgttttag cattagacga
     7621 ggatgattta tacgatcaaa aattgactga taatccaatt ggattcggct tgctacctga
     7681 agatgatgaa ggtaatgaat ggtttaaaat gacgcttatg aatgataatg gagacgaatt
     7741 atctgttgaa gatacttggt catatttgag tgattatatt gtcagcgttg aaatcattga
     7801 atttgtagcg gataaagaag aataaaaact ggaggaataa attttacaga taaaggctta
     7861 gaaggagtca cgacgattaa taaaaaggta gtattacctt aggcattgag taaggaattt
     7921 atttaggagg aaaattagat ggcgcgacca gttttagagg cgatggatat ggatactttg
     7981 acaaaaggaa ggtatgtaca gctgttagct cgtggttatt cacctaaaga tgtattcaaa
     8041 tccctgtcta aaggaacaga tatggaaaaa gaaagattac gaaaagaatt cgattattgg
     8101 agaacccata atggaatcaa ggatttgaaa ccagcacggc cagtaattaa acgaaagaaa
     8161 aagataaccc aagactgaag tgtagagcgt atgtgatcat aggctttttt atttttgcaa
     8221 aaaacaagga aggtaggaaa actcatgaat tatgaagaaa tcgaaaacag aaaaaaagtg
     8281 tcgaaagaaa tggaagaaaa gttgctaaaa acgatgaaac aaaaacacct taaaagatta
     8341 tctgtcatgc aatatatcaa tgatatgcag ataacaggaa aagagaaagc ttgtttgtta
     8401 ggttcaatga aaaactttga acagttgaga cgaacttatg tgaaaaaaag ttctaattgt
     8461 caactattgt tagaagtctc atgacattca ttaatagaat tcatttttaa aactttttct
     8521 atggaaatga gaaaaagatt ttttgttttg tctgaaaggg ggtagctgga tgagaaaatg
     8581 gttatttggt tttttaggag tcgctttaat cgtcttttgc agtgtgtttg gctatgttag
     8641 ctaccaaaaa catgaaggaa aggtgtttaa gcaaaatata gaaaaaaaga tgcccgtaga
     8701 tcaaatcaat gaacatgcaa agagttataa ggaggatgca acgaacgtca ataatgatat
     8761 gtctttgggg caaatgttgt ccatacaaaa agaagcaatc gaaatgggtg tcaataaaca
     8821 agcctttgca caaattcaga tacctgcact tggtttagca ttgccaattt ttaaaggggc
     8881 aaatcaatac actttgagtc ttggagcggc tacttatttt tatgaagatg ccgaaatggg
     8941 caaaggaaac tatgtcctag caggtcataa catggaaatg ccaggtgttt tattttctga
     9001 tattcaaaag ttgtcattag gggaggtgat ggacttagtt agtaacgatg gcgtctatcg
     9061 ttacaaggta acgcgtaaat ttatcgtact tgagtacttt aaattaatcg atggcgttcc
     9121 tgaagagaac tctttcttgt ctttaccaaa gaaaggagag aaacccttat taacgttgtt
     9181 tacttgtgtt tatacgtcac aaggaaaaga aagatacgtg gtacagggag agctacaata
     9241 acaaggtttt aatcaacgcg agtcgttgaa atatgaaatg aggagagtaa aaaatggcaa
     9301 taaatcggta tgtaaaaaaa gctggaatgc ttgttggaat gttaggaatc attgcaagct
     9361 gctttcaggg gtatcaagta cacgcagaag atgtcacgca aaaaacgcca ccagagaagg
     9421 tcaatatcac ggttcataaa ttaatgtacg atcaaggaac gcaattgaat gttgatattg
     9481 acgggatcaa aaacgatggt tatacgcacg atcaatatcc tacaggtgta acgaaataca
     9541 acaaggcaga ttatggtgat gttgagttta cgttaggaaa tattacggat caagtattac
     9601 cgagggaaga ttctgatcta actaacgcga aagtggacga aatcgtgaaa gacgtagaag
     9661 acaagggatc taacagtgag tacgtcaaga atgcaaccaa caaaattact tcagctgtag
     9721 atgaaaatgg tgagatcact tttgctgatc aaccagccta tgtcaattcc aaagggaatg
     9781 tatatgtggt ttatgaaagt aaatccgctg ctggattagt cacacaaaaa gcaaaaccaa
     9841 tggttgtgat tgcgccaatg actgataata caggttcagg ttttttaaaa gacattcact
     9901 tatatcctaa aaatattgtt tctaaattga gttttgaatt aacgaaattt ggtgacgatg
     9961 gcacagctca aagcaaacaa acacctttaa aaggggcaaa atttgagtta tacaagggag
    10021 aacctggaaa aggaacaaaa ttaggagatc ttgtatcgga tgatcaagga aagctgactg
    10081 ctactgattt gacgttagga aaatactatt ttgtggaagt tccttcagaa gttgtagtgg
    10141 gatcagataa agaaccaaca gcagatcaat atttattagg cgcagacgct cgcaacgatg
    10201 cacacaataa attaaccttc gaaattacga atgatggcgt gacaagtgac ttaaaagctt
    10261 cctatgtaaa ttacaaagct cctgtaatcg ataagacagt aatcaatgga acaggggccg
    10321 aacattcttt ccaaattggt gacgcagtga attatcaagg aacgattcat atccctacag
    10381 atattgcagg tggaacagat gggattacag taaatggtgt gaagagtgaa acttctcctt
    10441 acagcgtatt taagtgggga gatacagcag gtaaaggact ttcttatgtc gctgctaaag
    10501 caaatatcaa agtaacaaat aaagatggat cagtggtatt aaaagaaaat acagattata
    10561 agatccaaaa ttcagaaaat ggctttgtga tcgattttat cgtgaataat ggccaagtaa
    10621 gtgatactgt cgcaagttta cacggacaag atattcaact gacttataac atgtttgtga
    10681 atgatagtgc tgcaatcgca aatccattaa caaatagcgt ggatttcgtt tatagcaaca
    10741 atccattcaa tcaggaagaa caccatgaaa aaacgaaagc cgacgctgtg acttatggcg
    10801 caaaattctt aaaagctgat ggcggactat ttggaacagg aatcaaagca acaccgcttg
    10861 aaggcgcaga atttgctgtt aaaaacgcag aaggaaaata ttatggtgga ttagttgata
    10921 cggataaaga cggtgtcaaa gaagcagtat gggtagacga tgtagcaaat gcagcgatcc
    10981 taaaatcaga taaagaaggt cactttgaaa tcactggttt aactgaaggt gaatattcac
    11041 tggaagaaac aaaagcacca actggttacc aaaaattaac agaagatatt gcatttaaag
    11101 taaacaaaaa ctcatttaaa gaggaaaatc gaatcactgt taaaaataat caaaaagcag
    11161 ctattccatt aacaggtagt aatggtttcc aaacgtatgt attgatctct tgcttgttgc
    11221 taggagcagg ggcactttct gcagttgttt actttaagaa gaaagcctag aaggatcagc
    11281 taatgattat tcgtaagccc ccaaaaagaa gagtcatgca caaactgatg atattttttt
    11341 ggttgctgtc ggcagtagca gtctatttaa ttccatctgt ggaaacaaat gtagcgaaat
    11401 ttcaacaaca gcaaagaatt ttagccattg aaaatcttga aaaaacagat tcttctttgg
    11461 atgttgcgaa aatagagtta ggcgatcctg ttggtgttct tacaattcca agtatttctt
    11521 taaaactacc aatttatgac ggaacgtccg acaaaatttt agaaaatggt gtaggtatta
    11581 cggaaggaac tggtgatatt acaggaggaa acggtaaaaa tcctttaatc gcaggtcata
    11641 gtggacttta taaagataat ctttttgatg atttaccttc agtaaaaaaa ggagaaaagt
    11701 tttatatcaa agttgatggt gagcaacacg cttatcaaat cgatcggatt gaggaagtgc
    11761 aaaaagacga actacaaaga aactttgtca cttacttaga accaaatccc aatgaagatc
    11821 gagtaacgct aatgacatgt acgcctaaag gaatcaacac acaccgtttc ctagtgtatg
    11881 ggaaacgagt aacctttaca aaatcagaat taaaagatga agaaaatagg aaacaaaaat
    11941 tatcttggaa acggctttta ggaagtactg tttttctttc agtcatgatc attggttcgc
    12001 tatttgttta taaaaagaaa aaataaagga gggtaaccaa catgagaaat agaaaatggc
    12061 tacttgtttg tgcagtcgct cttttttctt tcactgccta cacacagtta acgtttgcag
    12121 acagtcaaga aagcacggat agcacagaac aaacaatcca aacgtcagat agcagcacga
    12181 ttcaaagcag cgatgaaacc gtcgattcgt caacagtgga ttcttcagaa gaaccgaaaa
    12241 ttgctgaaga cgatcaaata gtcaaacaga ccattcacgt tcaaaagatt atttcttcag
    12301 aacaattaga caaccagggg caaaagcttg aaaaacaaga aggagtaaat ggcgcgaagt
    12361 ttattgtcta cgatatcacg aatatcttag aagaaatgtc acaagatgat aatgagaaaa
    12421 atcaatctgt agaaataatt gagtcaaaat tgaaagatcg agcaaaaaaa ctttcatacg
    12481 accagttgaa aaaagtggct gaaggagaaa ccaaaacgat tgatgatcag gaaggcgtga
    12541 ttgaattttc cattgaagta cctgcaaaca aaaaacaagc atactatatc gttaatgaat
    12601 caagccctga aaacatttca aacagtgaag atattgtttt attgacacct gtttcaaatg
    12661 aaaaaggtga atttttgacg gatgtttggt tataccctaa atcagagaaa tcagagccga
    12721 aagaagaaat taaaaaagtt gtgtcaactg gtgtaaagaa gaactttttt gaaaattgtt
    12781 gggaattttt tgctcaatta ttttttgata attaggatga aatgatgaaa ataataagaa
    12841 tattagtttt taatctatcc tttttagccg caacaatatt gtgttgcggt ttaatttttt
    12901 ctcaattatc aatttatcca agatatcaac gattgttcat tcattttcta aacattcaat
    12961 gtgcttccat gccattagag gtgattaaaa agctcttgat caatccaatt tttatggtta
    13021 gtttggttct tcttacagga ttggtaacgt gggtattagg aaaaaaagaa tgaaaagctt
    13081 acgatcgttt aaaaaaatat tagatggagg gcattatgga aaataaaatt cattgcacta
    13141 cgggaagagt aattacttta gtgttgtttt tgttccttct tctttttgcg aggggaactg
    13201 cagtccaggc cttagaaaaa gttgatccga gtcaaatcaa agaaaataat aatctggtgc
    13261 tttatcatga tagtgattca ttgagaaata tcgaagtaac tgggatcaat ggatttaaga
    13321 tactagatga gtattcaact aaaaaacaaa aagtacttgc ttggccaaaa gtcaaagatt
    13381 tgaaaagtac gtcaaaaatc gttgcccatt atcaaaaagt aggtacttat ctaggaaaag
    13441 aaattgattg tgacgtgact ttttctaatt ttgtagcgca cgctggggtt aaccaagacg
    13501 gattagctaa aacaattaat ggcaaaaagt attataatta tttgctcttt gataaaaata
    13561 tttatcgagg atttttcatg cagtcaatca atgtcatgaa tgcaaaacta gaatttaagt
    13621 atgcaggaac aaaaacaaaa gtagctttga gtgaaccaca gccagaaatt caatcaaaaa
    13681 tgagatcgat gagagcagct ggtattgatt taaacgatac atttctatcc atttacagtt
    13741 taaatggaca gtatcagcaa cgtgtgtctt ggtataatga caataatgaa tctgtcggtg
    13801 aacgtaatat tgatatgaga gaatttgttg ggtatgaaaa aatgggtagc ttaccttatt
    13861 ttgtcacaac agatacagca tgtgcagaat acaaagctcc tgcgttatca acaaacaatt
    13921 taacttcaaa agtagtggga ggacgtgcag aaaaggctta tagctcgaat gatcatttca
    13981 ccgatgttgt aggagctgat acttatcaca gaagtggtgt aacgtatacg cttcaaggcg
    14041 cttccccaac attcatgatt ggcgcaaata cgaatagtat gatgtttagc tttgatactg
    14101 cattgctatg gacaccacaa ccatcgaagc ctacaaaaga agtgtttaac aaagctaata
    14161 ctgaagaggc agcacacaat attgacaaaa aagtgattcc acaaggatca gatgtttact
    14221 atcatattca ccaaaagttt gatgcattaa cagtcaacac aatgaacaaa tacaaatcat
    14281 ttaaaatcac tgataccttt gacagcaaaa attttgatat ggtatcggat gggaaaaact
    14341 atgatggcgc attgtggtat gttgaagcga atggtacttg gaaaaaagct gatacaggag
    14401 gaaagttaac tgtatcaggt aatacagtca cttatactgc ttctgacagc tttttagcta
    14461 acgctgcaaa ttacggtagg gattatattt tgcagctcca tatgagagca aaaaatgatg
    14521 caaaaggaat tgcaccaaat gtggctactg taacgtttaa caatgatccg aacaaaggaa
    14581 cacaaaaaac aaatacagta gaaaactatt tctctcttaa accaaataaa aaagtggaac
    14641 aagatgggaa agatgttgat ggtcgaaatg atggtaaaaa aggaacgcct acagctcctt
    14701 taaatgcagg atctgaagta caatatctag tcacacaaaa atggcataca aaaggtgtag
    14761 acattgcctc agatcattac aagcaattta gtattcaaga tccagtggaa gatcgtttga
    14821 cgtataaaga aggttctgcg cgagtgatag atatgagtac aggaaaagat attacttcag
    14881 aaggtacact aacctatgat agcaattcaa gaacgctgaa gtgggaagct tccgctgatt
    14941 tcttaagtaa aaatctttta gatggacgag aaattcaact gatatttaca gctaaaactc
    15001 ctttgaatga agaattggat atcgacaacc aagcaaaagt ttcagtagaa agtattgaga
    15061 aagcaactaa tgtagtaacg attggggtaa aacctaactt acctcagatc attgtgccaa
    15121 aaactggatc gaatcattta gtgctaactg ttattggaag tatattattt cttactttag
    15181 catcattggg ctatgtagtt ttaaaactca aataaatata aaaacaaggc tgagaatcag
    15241 ccttgttttc ttttatttga agattggatt cgcactaata aatacattat ctacgccaat
    15301 tctaccagca gagtcaacta caaaattatg acctaatccg agaatacttt ggttattata
    15361 aagtaaattt gcattatggg cagggctatt tttccatcct accatggcat cgatagcacc
    15421 atcgtctgat aaaaggtcat caatatacag cccagtttga cttatgtttt ctcccttagc
    15481 accgtagcag cctaagcttt ccaataaatc ttggaaagta ccaccaccag ggcgatagtg
    15541 actgaagctg acttgtaatt ctttagctcg aatatctgca gcctgttgaa caatatcatt
    15601 gaattgcatt ggggcaacac cattttgttg gcgataatca tttaaccaag caagttcttt
    15661 ttgacgcata aagttacgta attcgtctaa tgaaatcttt ttatatttga atgtgattgt
    15721 tttattttga tcgacgttat taatggatcg ttggccgttt ttatccacgg taacggtagg
    15781 atcgtcattt ctgcacaaga aataattttg atcagtcatg tttttccaag gagtagcaaa
    15841 gctatcccct tcgtatactg tttttgattc actagagaga acagcgttgg tatcagcatt
    15901 tacatgatta acagtcacat taaatttacg agtgtagttg aaaatataat tttgactttc
    15961 agtcacattg tttaaggtta ctgaatttgt tggttctacc cgatcgtcta aaacatatcc
    16021 ttgaatagaa ggagcagtta aagtgacact gtcaccgtga gtgacattga ctatctgttc
    16081 tttcaaaata ttaactcgtc cttcgttttt gtagttgtta ttgcacaaca aattgatttg
    16141 atgcacttga ttataattga atgtgattgt tgtattacct gtgattgttt tgctttgagt
    16201 atcattacca atcactttgt agctaaaatt atctttagcg ccttcgctat atttaaatgc
    16261 ttttgcctta gcagtgtaag tagaaccaga agttactttt gcttgatctg tagcaatcac
    16321 ttgacctgtt tgattgtcaa tatggttaat agtaaccgta tattcttttg gtgtttcagg
    16381 tttgtgttgc aaccctttaa atgcagcatc aagactattt ttagcattat ttacttgggc
    16441 ttgagaagcc ttagaatcgt ctagtacttt tttagcattg gataaagcag tctgaaaatt
    16501 attccaagat tcttctgtaa aatctccttt agcagttgat tttactcggt tgtataaagc
    16561 ttgtaattca gctttattca cagtaggaac tggttttttc tttaatcctt tgtaagcaga
    16621 atctaattta tttttggcac tatccacttg tttttgagtc gcttttgagt catttaacac
    16681 tttcttagca ttgtttaaag cggtttggaa tgcgttccag gtattgtcgg tatagtcacc
    16741 ttttttagtt cccttaacgc tcgtataaag agcttctaaa gctgatttat tcactttggg
    16801 tttagaggtg tcagcgccat accaaccgat accttcgtat ttccagccat agtctttggt
    16861 taaccattgt acttctttcc aacttgcagt gtagtgatgt gagccagatt ttacaccagg
    16921 aaggaaaagg cggtagatcg gtttattatt tttttcggca gaacgccagg caggcccttc
    16981 ataagtccag ccgtaatttt tagtcaacca tttcacttca tcccaactag ctgtgtaatg
    17041 gtgatcacga agacctttat tgtacaaacg atagacaaga tggccaccgc tagtcggtgc
    17101 ttcccaggcg gggccttcat aatttcccca ttttatcttt actagatggt cacgttcttt
    17161 tttgctagag gtgtaaaaat gttcatgatt atttggattg taaaggcgat acatgacgtt
    17221 agttgaagca gcgtcaacag atccagcaag actaagtgag gcagcaaatg ttccaatagc
    17281 taaaacagtt tttggattaa ataatttcat tttttcaact cctaaattta attaaaaatg
    17341 tttacatgat tattatatat cggctaaccg atatagtcaa taaaaaataa gaaaagcctg
    17401 atataatttt ggcttttctt attttatttg ttagttgaag tattaatatc aacgatttct
    17461 tcagccaatt tatttaattt catagcattt atgaatctca tgttgtctat actagagcgt
    17521 ttgttggcaa ggtcactgac tgtagttaag ggtacgccag attctatgga tatagaacga
    17581 actgatttac cactatcaaa cagcaaccat tcgattttct ttttatcagc gtacatttca
    17641 atgctccttc ttgttttttc tttattatag ctcttttttg taataaatca taatatcaag
    17701 agaaaaattt tctatagaaa ggtggatttt tatgagtatt cgttctccgt gtatccaaat
    17761 gaggtttgtt gctactttcg ttatttgata tactaaaaat aaacaacgaa agaaggaata
    17821 aaatgaagaa aaagcaacta tggtttgtct ctatgtttgc aatatttttt attattttaa
    17881 gtggatgttc aagtaataag aaatcggaag ctaaagaaga taatagttta ttgcaaccag
    17941 gaactgtttg gaaagaagaa gtaggtggtc ttattcataa tttaaaaatt atagatgata
    18001 ctacctggga atattctgaa agtgtatggc atccagatcc tgtacaaata acagttaaga
    18061 gacaaaaaga ctatgaaggg ttagaaagat acaaaatagt agattctgca ggtatcagaa
    18121 aatttataaa caaacccgat tctttattta tcgttgttcc ttacgagaaa gatggcgtaa
    18181 aaaaaattgt ttttcttgga tctagtaagg atgaaaagcg aacgaaagaa aaattgattc
    18241 atgatggatc acaatcaaat aaatacaaat tacaaaagac tagcgagtga ttgctagtct
    18301 ttttatttac cctaaaggag gaatcatagt ggcaaaagcg aaaacgccat ttgaaaaagc
    18361 cgcgtctaaa atcttagaac gtcgcggtgt atctgaaaca cagtggaaac aagaagtcct
    18421 tgaaaaagca caatttgacc tattacgggg aaaagacaag gacttagaag gatatatcta
    18481 tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt aaacactaaa aggagcgaac
    18541 aattttatgt ttgaatcaat tacaatgaat tttttaacac aaccgaaagt agtagctgcg
    18601 gttgatattt ttagagcatt acaaactgcc gcaactagtt tgaaaaataa tctgatgatt
    18661 tttgctgctg ttgcggcagt ggcttccatt gctcttggag gtattttctt cttctttggg
    18721 cgaagaggag ctgaaaacgg gaagacacac gtaggttatg tattgttggg ggtaggagtt
    18781 attttagctg ccgcttctat tgtagtttca atgttctcaa tgtttggaca aaccccacaa
    18841 ggactataaa aaaaacaagt agaaggagta aaaagtacgt ggctactttt tactcctttt
    18901 tttatgaaag gagaatattc atgtttttta cgatttcagg aaatgcaaaa ttggataaag
    18961 agttaataag aaaagcaacg aagaaagtgg gaaagcgtac ttccttttta ccgaaaacaa
    19021 ttaaatattc tgtcattgaa acggaagaac agctacatgt gttaggacaa gtgttagaac
    19081 aagagaatca atcttctgtt aaattgactc tcacgttaca tgataaagaa ggaaaaatag
    19141 aaaaggctgg cacagaatac gtttttggcc cactggatta tcaaatttca gaaggttttt
    19201 atcctacatt gatggattta atccgccttg atgtgtttaa agatacggat gaaactatga
    19261 gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt tttagaagaa gagttagaga
    19321 gcgaccaaag aaaagtgaat caagtgagtg aagtcgaagt aaatgaagaa gtaccatggg
    19381 aacgaccaat atttccaaag aaggagtcaa aagaaaaaga gacagtctat gttgacgaac
    19441 aagctgatat tccgactcag aaatcgatat ttgaggaaga gaacgatgtg atgggtcaat
    19501 tggaggtaga tccctttgaa gaaacaatgg aggatgaaga gacggacgaa gtagaagttg
    19561 atccagttga agctaatgaa gagtcaacat ggactgaagg ggattctcag gaatatattg
    19621 aaccacttat cagtgaagaa gtagaaaaaa acgaaa
//
LOCUS       DANKEF010000048        19609 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000048 DANKEF010000000
VERSION     DANKEF010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.048"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            127..3654
                     /locus_tag="PWZ48_002018"
     CDS             127..3654
                     /locus_tag="PWZ48_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HDL2261668.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="PWZ48_002019"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="PWZ48_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HDL2261669.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="PWZ48_002020"
     CDS             7442..7783
                     /locus_tag="PWZ48_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2261670.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="PWZ48_002021"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="PWZ48_002021"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDL2261671.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="PWZ48_002022"
     CDS             9140..11560
                     /locus_tag="PWZ48_002022"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDL2261672.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PWZ48_002023"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="PWZ48_002023"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDL2261673.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="PWZ48_002024"
     CDS             12994..13731
                     /locus_tag="PWZ48_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2261674.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="PWZ48_002025"
     CDS             13745..14560
                     /locus_tag="PWZ48_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HDL2261675.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="PWZ48_002026"
     CDS             14573..15220
                     /locus_tag="PWZ48_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2261676.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="PWZ48_002027"
     CDS             15236..15892
                     /locus_tag="PWZ48_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDL2261677.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="PWZ48_002028"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="PWZ48_002028"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDL2261678.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="PWZ48_002029"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="PWZ48_002029"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDL2261679.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="PWZ48_002030"
     CDS             18234..18752
                     /locus_tag="PWZ48_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2261680.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="PWZ48_002031"
     CDS             18859..19359
                     /locus_tag="PWZ48_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HDL2261681.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
BASE COUNT         6581 a         3369 c         4207 g         5452 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcactgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt agcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaaga
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttt
//
LOCUS       DANKEF010000049        19507 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000049 DANKEF010000000
VERSION     DANKEF010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.049"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="PWZ48_002032"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="PWZ48_002032"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDL2261682.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="PWZ48_002033"
     CDS             complement(1173..1868)
                     /locus_tag="PWZ48_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HDL2261683.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="PWZ48_002034"
     CDS             complement(1881..2597)
                     /locus_tag="PWZ48_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HDL2261684.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="PWZ48_002035"
     CDS             2927..3532
                     /locus_tag="PWZ48_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HDL2261685.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="PWZ48_002036"
     CDS             3857..4180
                     /locus_tag="PWZ48_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HDL2261686.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="PWZ48_002037"
     CDS             4362..5732
                     /locus_tag="PWZ48_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HDL2261687.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="PWZ48_002038"
     CDS             complement(5862..7163)
                     /locus_tag="PWZ48_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDL2261688.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="PWZ48_002039"
     CDS             complement(7393..9351)
                     /locus_tag="PWZ48_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HDL2261689.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="PWZ48_002040"
     CDS             complement(9528..9794)
                     /locus_tag="PWZ48_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDL2261690.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="PWZ48_002041"
     CDS             complement(10304..12430)
                     /gene="pnp"
                     /locus_tag="PWZ48_002041"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDL2261691.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="PWZ48_002042"
     CDS             complement(12618..12887)
                     /gene="rpsO"
                     /locus_tag="PWZ48_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDL2261692.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13138..13701
                     /locus_tag="PWZ48_002043"
     CDS             13138..13701
                     /locus_tag="PWZ48_002043"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDL2261693.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(13954..14475)
                     /locus_tag="PWZ48_002044"
     CDS             complement(13954..14475)
                     /locus_tag="PWZ48_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HDL2261694.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14478..15320)
                     /locus_tag="PWZ48_002045"
     CDS             complement(14478..15320)
                     /locus_tag="PWZ48_002045"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HDL2261695.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15313..16038)
                     /locus_tag="PWZ48_002046"
     CDS             complement(15313..16038)
                     /locus_tag="PWZ48_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDL2261696.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16214..16510
                     /locus_tag="PWZ48_002047"
     CDS             16214..16510
                     /locus_tag="PWZ48_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261697.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17078..18670
                     /locus_tag="PWZ48_002048"
     CDS             17078..18670
                     /locus_tag="PWZ48_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2261698.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18683..19423
                     /locus_tag="PWZ48_002049"
     CDS             18683..19423
                     /locus_tag="PWZ48_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDL2261699.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         5580 a         4002 c         3702 g         6223 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt attgtccccc
    10261 tgtcaccttc acgatcacaa gtacattatt cctatgaatt aaattatttt tcttcatttt
    10321 tcgttgaacc atcttcgttc aacattgctt ttcttgacag atttacgcgt ccttgtttat
    10381 cgatttcagt tactttcaca aggatctcat cacctagttt aacaacatct tcgactttat
    10441 tgacacgatc gtttgctaat tgggaaatat ggacaagtcc atcttttcct ttgatcaagt
    10501 tgacgaatgc accgaatttt tcgatacgga caactttccc tagataaact tcaccaactt
    10561 tgacttcttt ggtcaaatct tcgatgatct tgatagcttt tttgatcatt tctgcatcag
    10621 ctgaagcaat acttactttt ccatcttgat cgatatcgat tttcacacca gtttcatcga
    10681 tgattccgtt gatcgtatcg ccgcctttac cgataacatc tttgatttta gcaggttcga
    10741 tctggatcat ttcgattttt ggtgcatatt ggcttaattc ttcacgtgga gcagcaatcg
    10801 ttgaagtcaa ttcttctaag atttccatac gtgcttgttt tgcttgagtt aatgcttcag
    10861 ttaggatttg ttctgtgatt ccttgaatct tgatatccat ctgtaaagca gtgataccat
    10921 cttttgtacc ggcaacttta aagtccatat cgccaagatg atcttccagc ccttggatat
    10981 cagttaggat cgtatagttt tcaccatcag aaacaagtcc catcgcaata ccggcgactg
    11041 gcgctttgat tggtacacct gcatccatca atgctaatgt gccagcacag atacttgctt
    11101 gggaagaaga accatttgat tccaatactt ctgctactaa acggatcgta taaggaaatt
    11161 cttcttctga agggattact tgtgctaacg cacgttcacc taatgcgcca tgtccgattt
    11221 cccgacgccc tggagagcct gcacgtcctg tagaaccaac agaaaattga gggaaattgt
    11281 agtggtggat gaatcgtttg ctgacttcca cccctaaacc atcaatgatt tggtgctctc
    11341 ctagaggtgc gagtgtacaa gctgataaag cttgtgtttg tcctcgtgtg aacaaacctg
    11401 atccatgtac acgtggcaat aagctgacct ctgatgacaa tggacggatt tcatccaatt
    11461 tacgcccgtc aggacggatc ttatcgatgg tgatcaattc acgtacgaca tctttttcaa
    11521 gatcttctgt gatttgttta acttctttca acaagtgatc ttcatcttca tgacctgcaa
    11581 atttctcagc ataagcttcg cggatatcga tcttcacctg ctcgatatta tcttcgcgag
    11641 caagtttttc ttcagtcatt actgctgctt tcattgtagc gtaagaagca tcaaagattt
    11701 cttttttcaa tacttcatca acttgcagta aagtcacttc cattttttct ttccctactg
    11761 cttgaacgat ttcttcttgg aaagcaacta actctttgat agcgtcaaaa ccaaacagca
    11821 atgcaccaag catgtcttct tctgaaactt ctttcgcgcc gctttcaacc atattgatgg
    11881 cttgtttcgt tccggcaaca gtcaattcga tatctgtttg ttctgcttgt tctactgttg
    11941 gattcaagac gtactcgcca tttacacgac caacttctac gcctgcgatt gggccgtcaa
    12001 atgggatatc tgagattgat agtgctaaag aagaacctag cattgctgcc atagctggcg
    12061 agcagtctgt ttctacactc atcacgatgt tcgttacttg gacttcatta cggaagcctt
    12121 ctgcaaacat cgggcgaatt ggacggtcga tcaaacgggc agtcaatgtt gcttccgtac
    12181 ttggacggcc ttctcgtttg atgaaacctc cagggatttt ccctgctgca tacatttttt
    12241 cttcataatt gatcgttaat gggaaaaagt ctgtatcttt tgcttcttta gaggccacag
    12301 ctgcacttaa gactactgta tctccataac ggactaaaac ggctccgttt gcttgttttg
    12361 ctagctgacc gacttcgatt tgtaagggac ggccgcccca tgtcgttttg aatacttgtt
    12421 tttctgacat aacttctcct tttccttctc gtgaaagcaa gacgctacta aacaaatgaa
    12481 aactgccgaa agccgtcttt tgagcagctt tcatttggtt agtaggagtc catctagctt
    12541 gatcacggaa ttttacttaa ttgatatacg agtaaaaaaa gtgagattca tgtgaatccc
    12601 actctttttg actaaaatta acgacgcaag cctaaacgtt tgatcaattc gcggtaacgt
    12661 tgaacgtcag ttttacgtaa gtaagctaac aagttacggc ggtgaccaac ttttttcatt
    12721 aggccgcggt atgaatgatg gtcttttttg tgcacgcgtg catgttcatt caagtggttg
    12781 atttcttcag ttaatacagc gatttgtact tctggagaac cagtatcccc ttcatggcga
    12841 gcgtattcgt tgatgatttc attcttgcgt tcttttgaaa ttgccatttc tttcacctct
    12901 ttcgattatt cccctgcgac tgagtaaaac gttggtgatt cgcttaacca agtggcaggt
    12961 catgtgccta gagcacacag ctatacttta cctgaaaccg ggttgaaaat caagtagtta
    13021 atcattaaga aaaggtagtt ttcaaacaat cttactacta cttttccatt tttgtggtaa
    13081 actataatgc gaaaatagac ttgaaacaca gtcttttaag aaaggggtac atattccatg
    13141 attacaatgg atgatatcat tcgtgaagga aatccgacct tacgcgaagt tgcaaaagaa
    13201 gtttctcttc cattaagcga agaagatatc tctttaggaa aagagatgct tgagttctta
    13261 aaaaacagcc aagatccaat caaagctgaa gaactacact tacgaggcgg tgttggctta
    13321 gctgctcctc aattagatat ttctaaaaga atcattgctg ttcacgtacc aagtcctgac
    13381 ccagaggcag atggaccttc tctaagtact gttatgtata atcctaaaat tttgagccat
    13441 tctgttcaag atgcttgttt aggggaaggt gaaggctgtt tatctgttga tcgagaagta
    13501 ccaggatatg ttgttcgcca tgccaaaatc actgtgtctt attatgatat gaatggtgaa
    13561 aaacacaaga ttcgtttgaa gaattatgaa tcgatcgtcg ttcaacatga aatcgaccat
    13621 atcaacggtg tgatgttcta tgaccacatc aacgatcaaa atccatttgc actaaaagaa
    13681 ggcgtattgg tgatcgagta gagaattggc aggttccttc gatactatgc tacaagaaag
    13741 ctttataaaa aaggggagtg ctcaaaagct gcacttccct tttttatgtt tctccactaa
    13801 tcgaaattta gatagacatc tagaaatcgc aattccaaaa aattctgtgt attggatacg
    13861 catcttttct tattttttct tatttcttcc atatgggtgt ttatctcaga gatgatagaa
    13921 tagtttctag gataaaaagt agcagctacg atgttatcct tcgtagagga ttcctgcttc
    13981 agctagtttt tgtttgatgt ttttcagcat atgcttttct gggtagcgga tcgtatgcag
    14041 atggatgccg cctgttaatt cggataacaa agtagcatta cttttttgat aacgtctcat
    14101 aaaggaatcg atatcttgtg gcgttttgat gtgcaaagtt cctgctaact caccgtaaag
    14161 gggatgttcc acaattacat caatgatttc accaccattt tcaacgatta gtctcagttc
    14221 ttcttctgtt tgatcttttc catgctggac agcgatttta ctgatgtgcc cagtattggt
    14281 ttcttctgtt tcgaggatat aacctcttgc tgttgccacg atacttttac ctgcagctcg
    14341 tagtaaagca atatcgccta caatgatctg acggctgact ccaaaagttt tcgcaaactt
    14401 gctagcactc actggttttt ctgtatttgc caaggtttgc atgatttgtt ttcgccgtgt
    14461 ctctccttcg atcacactta ttcctccttc attttgtttt ggtattcgcg cgagtgtttc
    14521 atcaaatcaa catacgtgtc tgttattgtt tccttatatc ccttattttt cagcagacta
    14581 acaactttat ccaatacttg ctgctctctt tttgcatcat aaactgcttg gttttcttct
    14641 tttttcactt gtcctatttt agttaccgta tccattcttt tttctaataa agcaaccaac
    14701 tctcgatcga tcatatcgat cttatgacgt aatgtttcaa gagactcttc tgttagttct
    14761 tcctctactg tcttgatgat acgagcagga tttcctgcaa tcactacatt gtcgggaaaa
    14821 gatttggtca caaccgaacc tgcgccgact actacgttgt cccctagagt gacacccggc
    14881 gtgacgataa ctcctccgcc taaccaaaca ttattaccga ttttaatggg ttttgcgtac
    14941 tccagaccgc tgttacgttt cactggatgt agtggatgcg tcgccgtgta aagctggaca
    15001 ttcggagcga acatgcagtt atcaccgatt tcaatcgtgc tgacatctaa aaatgtacaa
    15061 ttgaaatttg cataaaaatt ctccccaaca aatatattat atccgtaatc aaaattgata
    15121 actggctcca tatagatttt tttccctgta cggccaaaag tctctttcaa caattgggat
    15181 cgcagttccg ctgattctgc ttggttgatg atttggcttt gacttcttgc aaacatacgg
    15241 tcggcaacca gctcaggatc attggcaaaa taaagctcgc ctgcaatcat tttttctttt
    15301 tcggttttga attcagtcat aatctgctcc taattcttgt atttttttga aaacaaccgc
    15361 tccgtcttct tgaataaacg tataaggctc cgctaaataa acaggcgttt gattgattac
    15421 taagattttg gctgacggct ggcgaaactg taccagatca caaaacggat gaacttgaaa
    15481 actcgttcca acaatgacga tcagctctgc ttggctgacg gcataagccg cctgctccat
    15541 gactccatct tgaaaccctt caccatataa cacgatgtct gggcgtattt gtcctccgca
    15601 tgcttgatgt ttgtcgctgt gaagatattc tttccagtct accgtttgtt gacattttcg
    15661 acaatgacat tgatacagac tcccatgaaa attgacgagt tgcgggcttt tagcctttgc
    15721 gtgcaaaccg tcgatgtttt gggaaacaac ccaaacattt ttttctttcg ctagatgtgc
    15781 tatttccaaa tgaatgatat tgggttttgc ttcaggatga taaatctttt tgacaaattg
    15841 atagaattta tccggttcat tcaccattgc ttgatgactc agcaagtatt ccggctgctc
    15901 catcccgtgg tagattcctg acaaagatcg atagtctggc actccagaag gtgtggatac
    15961 ccctgcaccg gtcaaaaatg taatttgctg ataagctaat atctcttttg ctgcttcgtc
    16021 tactgttaat tgtttcatgt tttgtcacct cactttccat tatagcccat actcgaacaa
    16081 atttattgtt gaaaagtcag aaaaatggga ataaaaaagt gttatgggca tttgagcaat
    16141 ttatcacaat gttcactgac aaaagaatga aaacgtgtta caatactgct gtataaaagt
    16201 gaggtgaatc gatatgttac cattctttat ttattggggc gttattctgg cttgtatcgc
    16261 ctggttagcg ttaagcgttt atttctctgt tttttatctt gtacgcaaag aaaatggaaa
    16321 tctttgggct tttgctttat tcaacgtgat tgccgcaatt gttttagcca ttacattagc
    16381 cgtatatcga acatggggct ggggaatcac ccaatactca agtttgattt atctaatatt
    16441 agccatttat ggcgtcgttg ttcttttgca agcgatccta ggacgtgagc caaagaaagc
    16501 ggcagcttaa atgatcgaga tggaactgga cacaggaaat tttctgttct agttcttttt
    16561 ttattgtatt acatacattt ttgtgtataa ttatactatc gtttgcaatg gatagatgat
    16621 tcacattttg attgtttttt cggtaaaagt aaagatttct atatttatca gtattttttc
    16681 aaaaaccagc agttatagat tgacaaagcc tctcgagtat cgtaaacttt ttttgtataa
    16741 ataactgaat acgagatgcc tcaatcaatg aggtagaggt cgcgagaatc attaaccctg
    16801 tggagtgaag caaacatgga gaagcaggaa tagggaggat cgccgaaatg tgcacttttt
    16861 tgcttggagt gtatgttggg acgcaagtta agagcttgcg gactgtctta gcagtgatgc
    16921 taagttgcgc tatgttttgt gagaggttta tcaccttatg gaattttagc ggccttttta
    16981 tatctataaa tcggtcgctt tttatttttt cagataaagc tttaccgcat ttcatctaga
    17041 ccgctcgtgt atcatataat cagaaaggga gaaaaaaatg aaaaaaaata tggtttcagt
    17101 tgccgtacta tttgcttttg tcttctcact attcagtgtg ggatctctgg caaatgcaga
    17161 tgaaaaaaca ccgagcgatg agtttcgcgt aggtatggag gctgggtatg cacccttcaa
    17221 ctggtctcaa caaacagatg agaataatgc cttgccaatt caaggccaaa atagttacgc
    17281 cggcggttat gatgttcaaa tcgctaaaaa agtagcagat ggtttaggga aaaaattagt
    17341 catcgtccaa acgaaatggg atggtctggc tcctgccctt caatcaggca agatcgacgc
    17401 gatcatcgcc ggaatgagcc ctactgctga acgacgaaaa gaaattgatt ttaccgatcc
    17461 ttattacgaa tcacaattag tagtagtagt tcaaaaagat agcaaatatg caaaagcaaa
    17521 aagtttagca gatctttctg gagcaaagat cactgctcag ttaaatacat tccactatac
    17581 tgtgatcgat cagataccag atgtgcagaa acaacaagca atggataatt tctcagcaat
    17641 gagaacagct ttagcttctg gaatgatcga tggttatgtc agcgaacgtc cggaaggtgt
    17701 aacggcaact agtgtgaaca aagacttgga aatgcttgaa ttcagcggtg cagatggctt
    17761 ccagacaaac ccggaagatg ttcagatttc tgttggtatg cgtaaaggcg acccggatat
    17821 ccaaaaagta aaccagattc tttctggcat ctctaacgat gaacgaacca aaattatgga
    17881 ccaagcagtc aaagatcagc ctgctgcaac tgatagtgaa gaaaaacctg gcatcatttc
    17941 tgatttcact acgatttgga aacaatacgg caatatgttc ttacgaggtg ctggacttac
    18001 cttgttcatc gcattgatcg ggacagttgt cggtactaca cttggattac tgattggtgt
    18061 cttccgttcg gtacctgaat caggaaatgc actgactcgc ttcttccaaa aaatcggaaa
    18121 tgcacttctt tctatctatg tcgaagtatt ccgcggaaca ccgatgatgg tacaagcaat
    18181 ggtcatcttc tacggtctgg cactcgcttt tggcatttca ttgaaccgaa cgattgccgc
    18241 tttgtttatt gtctctctca atactggagc ttacatgaca gagatcgttc gtggggggat
    18301 ttttgcagta gatgaagggc agttcgaagc tgcgcaagcc attggtatga cccatggcca
    18361 aacgatgaga aaagttgtta ttccacaagt tttacggaat atcttgccag caacaggaaa
    18421 tgagtttgtt atcaatatca aagatacagc agttttaagt gtaatcggcg tagctgattt
    18481 gttcttccaa ggaaactctg cttccggtgc gaatttccaa ttcttccaaa catttacgat
    18541 cgtcgggatc atctacttag tcatgacctt tacgatcacc agaatcctgc gggcggtaga
    18601 aaagaaaatc gatggtcctt cagcttatac caagcttgaa gaaatcgaca attcaaattt
    18661 gcaggaataa ggaggaagaa tcatgaatga aaaaattatt gaaatcaaac atctaagaaa
    18721 aagcttcggt gaaaatgaag tattgaaaga tatcagcgta acagtcaata aaggtgaagt
    18781 tgtgacgatc atcggttctt ccggttctgg taaatctact ttcttgcgct gtatcaattt
    18841 actggaaaaa ccaacaggcg gcgagatcat ttataatgga gaaaatgtat tagctcctaa
    18901 atataattta cctaaatatc gaacgaatct aggtatggtt ttccaatcct tcaacctgtt
    18961 caacaatatg aatgtcttgg aaaactgtat gtctggtcaa acaactgtcc tgaaacgaga
    19021 taaagaaact gccaaaaaag tagcaatcga aaatctggaa aaagttggta tggatcgtta
    19081 tatcgaagcc aaaccagcac aattatctgg tggacagaaa cagcgtgtcg caattgctcg
    19141 ggcattatca atgaatccag atgtattgct attcgacgaa cctacttcgg cgttagaccc
    19201 tgaaatggtt ggcgaagtgc ttaatacgat caaagactta gcacatactg gcttgacaat
    19261 gcttatcgtg acgcatgaga tggaatttgc aaaagatgta tctgaccgtg tcatttttat
    19321 ggataaaggt gtgattgcag aagaaggtac accagaagat atctttgtcc atccgaaaga
    19381 agaacgtaca aaagaattcc tttcacgtat tttaaatgct taggatcaat agctgattgg
    19441 gacaaagagc tgcggtgttt accgcagttc ttttttatga attactcata aaaggtaaat
    19501 ttctgaa
//
LOCUS       DANKEF010000050        18950 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000050 DANKEF010000000
VERSION     DANKEF010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.050"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            312..1091
                     /locus_tag="PWZ48_002050"
     CDS             312..1091
                     /locus_tag="PWZ48_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261700.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1137..2081
                     /locus_tag="PWZ48_002051"
     CDS             1137..2081
                     /locus_tag="PWZ48_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2261701.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2097..2876
                     /locus_tag="PWZ48_002052"
     CDS             2097..2876
                     /locus_tag="PWZ48_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HDL2261702.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2888..3586
                     /locus_tag="PWZ48_002053"
     CDS             2888..3586
                     /locus_tag="PWZ48_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HDL2261703.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3614..4378
                     /locus_tag="PWZ48_002054"
     CDS             3614..4378
                     /locus_tag="PWZ48_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HDL2261704.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4422..5873
                     /locus_tag="PWZ48_002055"
     CDS             4422..5873
                     /locus_tag="PWZ48_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2261705.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5905..7041
                     /locus_tag="PWZ48_002056"
     CDS             5905..7041
                     /locus_tag="PWZ48_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDL2261706.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7041..8147
                     /locus_tag="PWZ48_002057"
     CDS             7041..8147
                     /locus_tag="PWZ48_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HDL2261707.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8153..9175
                     /locus_tag="PWZ48_002058"
     CDS             8153..9175
                     /locus_tag="PWZ48_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HDL2261708.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9197..10252
                     /locus_tag="PWZ48_002059"
     CDS             9197..10252
                     /locus_tag="PWZ48_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2261709.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10870..12105
                     /locus_tag="PWZ48_002060"
     CDS             10870..12105
                     /locus_tag="PWZ48_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261710.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12189..13277
                     /locus_tag="PWZ48_002061"
     CDS             12189..13277
                     /locus_tag="PWZ48_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261711.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13274..14695
                     /locus_tag="PWZ48_002062"
     CDS             13274..14695
                     /locus_tag="PWZ48_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2261712.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            14731..15447
                     /locus_tag="PWZ48_002063"
     CDS             14731..15447
                     /locus_tag="PWZ48_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HDL2261713.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            15466..17049
                     /locus_tag="PWZ48_002064"
     CDS             15466..17049
                     /locus_tag="PWZ48_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HDL2261714.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            17184..17762
                     /locus_tag="PWZ48_002065"
     CDS             17184..17762
                     /locus_tag="PWZ48_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261715.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            17708..18226
                     /locus_tag="PWZ48_002066"
     CDS             17708..18226
                     /locus_tag="PWZ48_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261716.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            18234..18878
                     /locus_tag="PWZ48_002067"
                     /pseudo
     CDS             18234..18878
                     /locus_tag="PWZ48_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
BASE COUNT         6906 a         2390 c         3693 g         5961 t
ORIGIN      
        1 ctattattaa ttcaatgtta atttttttgt tattctattt tttgtgtaac ttttaaaaac
       61 acctatgaaa attatggaaa taaaaatgaa atcgattaat ttttatctta ttaatgtttt
      121 atttactttt aaatcgtaaa ttgccctagt caagaaaaca gtttgtggta tgattatata
      181 gggaaaactt gtttacaaat atttctgtga atatattagt ttaattatgt ttttttaaat
      241 aaaaagctgt catttttatg taagtgaatt atatcttaat ttatctatca tcaatactta
      301 ggagtgaatc tatggaaaag ccattcgtca agtcattgac tgctgtggcg gtaggtgcag
      361 gtgctattgc gatctgtttg tttggatacc atatcaataa tcagcggcaa catcagcaac
      421 ggatcaatta tgcagaatct gccatcacca atcaaaaaga caccttgact tctttatcaa
      481 aagaagtaga caagctgtat agtacaaaag aaaaaatttt tctaaaccct gaaataacag
      541 aagaaacaat cacaagtttg tcaaataagc tatcctctgt caaactttca gcagatgatt
      601 acgacatcaa agaatcagaa ttgcctaaag aagctgttgc aatccaagat gagaagaaag
      661 caatcgtgac acagttagat agtgcagaat caaaactgaa gatccagaca gctgtcaacg
      721 atttatttac taaaaatgta tcaaactggc agcaagctgt agacgatgtc atcatcaaag
      781 aaaaggtgac aagtgcagat gtcgctcgtg tacgtgaaaa tatgagcttt tttaaagata
      841 gtccttggaa aacggttgtt atgcaatatc taggattcgc tgatattcag atcgcgcaag
      901 tcacgcaact agatcagtta ttcgatacga tgttaaagga tggtcaagtg actgccaccg
      961 ctacttacga tcaatatttg acggtgctca gtcaaataga gcagatccgg aatgaaaaaa
     1021 taagcgcagc atacgcaaca aaagcacaaa ctgttgctga gcaaatgggc tatagcaata
     1081 taagctattg acagttaaaa cgttgattca agcaaggtta gcaaacggag gaaactatgg
     1141 caagacgaag aagaaaatca agagggaaaa aaatatttct gattatttta gcagttctta
     1201 tcgtattagt attagcaatt gtaggagtag gagcaaaatt atatctggat ctctcacatt
     1261 ctattgagaa aacttacgaa tcagtggaac gaacccaagg agaacgaacg aaaccagttg
     1321 atctggacaa acaagaatct ttctctgttc tattgatggg gattgatact ggagatttag
     1381 gtcgggtcga tcaaggaaga tcagatacga tgatggttgc cacagtcagt cctgaagaca
     1441 aacagacaac gatcgtcagc attcctcgtg atacgtatgt cgacatcgta ggtcgtgaca
     1501 caaaagacaa gatcaaccac gcttacgcat tcggcggagc tgccatggcg atggataccg
     1561 tacaaaaata tgtagacatc ccaatcgatc actatgtctc tatcaatatg aaagggctaa
     1621 aggaactcgt tgatgcagtt ggtggtatcg aagtcaataa tgacctcact ttctcacaag
     1681 atggctatga ctttccaatc ggtaaaatta cattgaacgg ggatcaggca ttatcttatt
     1741 ctcgtatgcg ttatgaagac ccaaatggtg attacggtag acaagggcgc caaagaaaga
     1801 tcgtagaagg cgtagcgaaa aaagtattga gtctaaacgg tgtcagcaac tatcaatccg
     1861 ttttgaatgc tctagagtcg aatatgaaaa cagacatgaa ctttgatgaa atgaaaaaaa
     1921 ttgctttaga ttatcgggag gcatttggta cgattaagca agatcagctg caaggagaag
     1981 gcttcatgca agataatatc tcctatcaaa aagtcagtga tcaagaactt gatcgtgtga
     2041 aaaaagaatt aaaagatcaa atgaatctag agaataaata ggaaagtgga gaaaaaatgg
     2101 aagagacaat cagcttgaaa gaaatttttg aaacattacg caaacacttg gtaataatca
     2161 tcatctcaat gtttgctggc ttggccatct caggaatcgt aacatttttc atcttgacac
     2221 caaaatacag ttcacaagca caattgatcg tgacactgcc acaatcagac actgcaaatg
     2281 tcaatgatgt caatacgaac ctgcaaatga tcaatacgta taaagatatg atcgtcagtg
     2341 atttagtgct caatgaagtc aaagatcgtt tagaaacaga agacaatgtg aaaatgactg
     2401 ctgaacaaat caaagatgcc atctcagtta atcaggcaga aaattcacaa atgttctcga
     2461 ttcaggcaat aagtacgaat gcagtggctg ctcaacagat cgctaacaca acttcacaag
     2521 ttttccaaga gaacgcaaaa gatgtgatga atgttgataa aatctcgatc atttcaggtg
     2581 cagttgccaa tacgacacct gtttcgccaa ataataaatt gaatttaatg actggcttaa
     2641 tgttaggaat gatggtaggt gtaggtctgg cattcttatt ggaattactc gatcggacag
     2701 tgaaagatga aaaatatgtg acagatacat taggattccc aatcttagga acagttccag
     2761 aaatgtcagc gaaagaattg aatgctggta tccgtaaaaa aggaccagct cagacacaac
     2821 caaaagctac aaacgataaa acagcgccat cacgcagaag ccgctcaaga gtctaggagg
     2881 aaagaaaatg gcacgaacac agaaacaaaa agcagcccaa actcatgcag tcagtttggt
     2941 cacactcacc aatccctctt caccaatcgc tgaacaatat cgtacgattc ggacgaatat
     3001 ccagtttgcc tccgcagctg gtcagcaaat caaaacgatt gttgtgactt catcaggacc
     3061 tggagaaggg aaatcgacca ctgcagcaaa cattgccgtt gtttttgcca aatctggtca
     3121 acgggtgctg ctagtcgatg cagacttacg taagcccgtc atctataaaa cgttcaagtt
     3181 gaacaacgca agcggattaa gtactgttct tagttcatca ggaagtgtag cagatgagat
     3241 ccaacgaaca ccagtcgaca atttgtcgat tctgccaagt ggacctaaac caccaaatcc
     3301 atcagaacta ctaagttcgc caagaatgga tcagatttta gcagaagcaa gacaactatt
     3361 tgatgtagtg atcttcgaca tgccgccagt cgttgccgta acagatgcac agatcatgtc
     3421 ttcaaaaacg gatggcacct tattggtcgt acgggaaaat acgtcacgaa aagaatcatt
     3481 gactaaagca aaagaactgt tagatatggt acaagcacga gtcttaggtg tcgtatacaa
     3541 cggagcagaa cacagcaaag atgctggtta ttactacgga aattaaggaa taagaagaga
     3601 ggagacaaga gaaatgatcg atctacattg ccatatccta ccaggaatcg atgacggggc
     3661 agaaaactta gaagcaagta ttgcaatggc tgaaaaagcg attcagcaag gcattacgca
     3721 tattttatgc acaccacacc acaataatgg aaaatacagc aatgaaaaat cacaagtcat
     3781 ttctctagtc gcttcgttac aagccgaatt agagaaaaga cagttgcctt tgacgttatt
     3841 ggaaggacaa gaagtacgta ttaccggaac gctcatcgaa gacatccacc gagatgaaat
     3901 tttatttaca gatttagatg atacatatct tctgatcgaa tttccgacac tggaagtgcc
     3961 gttatatgca gaacggcttt tttttgagtt gttagaacta ggaaagatac cagtgatcgt
     4021 ccatccagaa cgcaacgcgc attttcgaga agacccaaat catttgcttc cgttcctaga
     4081 tatgggatgt ttggcccaac ttactgcgcc aagttatgtt ggtgtatttg gcaaagatat
     4141 ccagaaaaca gccaaaacaa tggtcaaaca taatctcgtg cagatggctg cttctgatgc
     4201 acacggggtc tctaagcgca ccttttactt aaaagagtgc tatgagcaga ttgccaagga
     4261 gtttggtaag gaaaaagtag tacagatgaa acaagtcgca aaggatctga tcaacgggga
     4321 tgagatcaat tatccaatct atgaagaggt aaaaaagaag aagtttggtt tattttagtt
     4381 ctttattcgt agataaaaag gaaatgagga gagagtgaga gatgtataga aaaaactcaa
     4441 gtggttggtc taaacatatt gattttattt tattagactt attttgtgtc cagcttgctt
     4501 tttatatatc atatgtaatg cgacagggtg actggaatcc gtatgtgatt cctatttatc
     4561 gccacatggc aatttttata gaacttgaga atcttacgat tatattttta tttgaggcat
     4621 ataaaaatgt tttaaaaaga ggatattata aagaggctgt agcttctata aagcaatctt
     4681 ttatgctgtt acttgtgtgc agtttatacc ttattacaat gcaggatgga aatgattatt
     4741 ccagagtttc tttatttata atgggggtgg tgtatggatt attaacatat ataattcgta
     4801 tactttggaa agggttgttg catcaccgca tgaaaaacgg tgaagatgta tcgttagtta
     4861 ttgtaacatc tcaaaagatt gcaaaaaatg tagttcgtaa tcttaaagag aagaattatg
     4921 gcatgtataa acttagtggt gtgattgtag tagacagaga tatgtatgga actaccatcg
     4981 gaggtgtccc agtagttgca aatgctgata atgcagcgga atatctttta cagaattggg
     5041 tagatgaagt attcatcaat gttgacgaaa gtgttccata tcccaaagaa ttaatcgcca
     5101 gatgttcgga aatgggactt gctgtacata tgaatcttac aaaagtgtct gatagtacac
     5161 gtggaaaaca atttattggc cgagttggtg attatactgt tattacatct agtattaatg
     5221 ttatgacgat gaggcaagca tttataaaga gaacgattga cattcttgct ggaattattg
     5281 gatgtattgc aacaggtgtt atttttatct tcgtagcacc agttatttac atcaaatcac
     5341 cagggccgat tttctttgca caagaacgta ttggtcaaaa tggtaagaca tttaagatgt
     5401 ataagttccg tagtatgtat atggatgcag aagaacgcaa agcagagttg atgaaagaaa
     5461 ataagatgag taataatctc atgtttaaga tggattttga cccaagaatc attggcaata
     5521 aaatacttcc tgatggaaca aagaagacag gaattggaca atttattcga tctacatcat
     5581 tggatgagtt tccacaattt tataatgtcc taatgggaaa aatgagcctg gtggggttta
     5641 gaccttgctt gaagtcagaa tatgagaagt atgattttca tcatcgtgcg agaattgcga
     5701 tgaaacctgg gattactgga atgtggcagg tgagtggtag aagtgatatc acagacttcg
     5761 aagaggttgt aaaacttgat gcagaatata tcaggaattg gagtatggga ttagatttta
     5821 gaattttgtt taggacagtc aaaactgtgc ttcatagaaa cggttccatg taagataaag
     5881 atactataga aggagtaaaa cgtgatggca agaaaaaaat taaaaatctg tatgctaggt
     5941 cataaaagaa ttccatcaag agaaggtgga attgagatag tggtagaaga attggcaacg
     6001 cgaatggttg cacttgggca tgaagtgact tgctataatc gaagtggtca tcatgttagc
     6061 ggaaaggaat ttgaccagca taaaaataaa gaatataaag gtgtaaaatt aaagactatt
     6121 tttacattaa atataaaagg aattgcagca atgagttctt cagtatttgg tggaattaga
     6181 gcagcattcg gaaaatatga tattgttcat tttcatgcag aaggaccatg tgctatgttg
     6241 tggttgccaa aactttttgg gaaaagatgt gttgcaacaa ttcatggtct agatcatcaa
     6301 agagaaaaat ggaacaagct tgctagtaca tacatcatgt taggggaaaa gtgtgctgta
     6361 aaatttgcag atgaaataat tgtacttagt gaaagcgtcc aaaattattt tgaagatata
     6421 tatggaagaa aaacaaggtt tataccaaat ggcgttaaaa aaattgaaat aaaatcagca
     6481 ggattgatta cagaaaagta tggattaaca aaagattctt atattttgtt tttggggcga
     6541 ttggttcccg aaaaaggaat tcggtatcta attgaggcat ttaaagatgt tcagacagat
     6601 aaaaaactta taatagccgg aggaagctct gatacagatg aatttgcaaa tgaactaaag
     6661 gaattagcaa aaggtgatga gcgaattata tttactggat ttgtacaagg acaggaatta
     6721 gaagaattat atagtaatgc gtatatctat acacttccaa gtgatttaga aggaatgcca
     6781 ttaagtttgt tagaagcaat gagttatggg aactgttgta tagtttcaaa tatctcagag
     6841 tgtacggaag tagtagaaga caaggctatg atttttaaga aatcggatgt aagtgattta
     6901 aagataagat tagaagaggc atgcaatcaa tcggaaatgg ttaaagtttt gaagaatcaa
     6961 gctactgagt ttatttgtag taaatataat tgggataaaa tcgtacaaga aacattaaat
     7021 ttgtataggg gaaatatata atgattcgaa tattacagtg tgtgaatgat atgcatcgtg
     7081 cagggttgga gacgatgcta atgaactatt ataggaatat agatcgaact aaaattcagt
     7141 ttgatttttt gacacatcgt ccatataaaa gtgattatga tgaggaaata ataagtcttg
     7201 gaggaaaggt ctattatgca ccgagacttt atccacaaaa ttatcctaaa tattttaaat
     7261 ggatggaaca attttttaag gatcatccag aatatgagat tgttcattct catattgatg
     7321 cgatgagtta tttaccttta aaagctgcaa aaaaagctgg gattccgata cggatagcac
     7381 atagccataa tacatcgata gatagagatt ttaaatatct tttaaagcaa tattatcgat
     7441 gtaagttaac gtctgttttg acacatgaat tttcatgtgg aaaagtagca ggtgattttt
     7501 tgtttcgaaa tgataaattc actttgattc caaatgcagt ggatgctaaa aaattttatt
     7561 atgatgaaag tattagaaat gatgtacgaa tgaaattagg aatttcaagt cagacgtttg
     7621 taatgggaca tattggaaga atttcatatt ctaaaaatca tcgttttttg attgaaattt
     7681 ttaaagaatt tcacgctatt aagaataatt ccgtattatt aattattgga actggtgata
     7741 tggaagaaga aataaaaaat tatgcaaaga aaagtggcat agatgatgat attaaatttt
     7801 taggaaatag aaatgatata gaaaaatttt atcaagcatt tgacgtatta atgttgccat
     7861 cattatttga aggggttccg ttagtgggta ttgaagctca atttgcagat ttaccgtgtt
     7921 tcttttcgga gaaagttcct acagaagttg ctttcagtga taagaccaat ttcataagtt
     7981 taaatcaatc ggcaaaagaa tgggcgagag agattgcaaa tgtagatatc tctcatcgag
     8041 attcagaacg ttcgatttta ataaaggcag attataatat tcaaacagca tacaaaatct
     8101 tagaatcaaa atattatgag ttatttgaat taatacaaag aggttaaaga atatgacaca
     8161 gaaacattca agtataaaaa gaattataca tcgcatatcc gaagaattga atgcaacaag
     8221 acgaattgca aagataacat cttggaagga agcatttgcc acgttacaag ctaaaattga
     8281 tattcaagtc atgaatcgta atggatataa tgaaagtaaa aaacagaagg aacatttatt
     8341 aaaaaaacat gaggtaatgc tgaagtatta tgaaaagact ttttcagatt ttttgaaaac
     8401 atatgattat agtcaaaatc aaataattaa tcaatcaaaa tcaccatatt ctaactgcat
     8461 ttgggtttgt tggtggcagg gattggatca atcgccggaa attgtaaaag cttgtgtaaa
     8521 atcaataaaa cgaaatgcag ggaaacatac tgttataatt ttaacagaag ataattatag
     8581 acaatatgtt gatataccaa aatgggtgga agaaaagaaa aacaaaggaa tcattacgag
     8641 aacaaattat tctgatttat taagattatc tttgctagca aaacatggag gtttgtggtt
     8701 agacgccaca ttttattgta caaaaccaat accagatgag tattttgata tcgatatttg
     8761 gtctattagg aggcctgatt atgctcacgc atccgtagca tcaggatatt ttgcgggtta
     8821 ttcattagca tgtaatcaag agtctagatg gatgtttgct ataattagag actttttttt
     8881 aaattattgg aaaaataatg atatgatggt ggattatttg atgatagatt atatgattgt
     8941 tttagcacag agaataaatc atgaaatagc acagacattt gataaaatac ctagtaacaa
     9001 tccattatgt gacgaattga ttaaaaattt aaataatata tatgatgaaa aagtatggaa
     9061 cttattaaag aaagatactt tactgtttaa gttgacatgg aaacaatctt ttaaaaagca
     9121 aaaagatgat aaagttactt tttatgggat gttggtagaa aacaaattat tataaatatt
     9181 agaggtataa aaagttatga aacacaaggt aagtgtgata gtaccaattt acaatgttga
     9241 aaaatattta agaaaatgtg tggatagttt attgagtcag acattaaaag agatagaaat
     9301 cgtattagtt gatgatggtt cgccagatgc ttcgggggaa atagcagatg agtatcaaaa
     9361 aaaatattct aatgtaaaaa caatacatag agaaaatggg gggttaggac cggcaagaaa
     9421 tacaggaatt gagaatgcga caggagaata tgtggctttt cttgattcag atgattgggt
     9481 agaaagtgat atgtatgaaa aattatactt agttgcgtcc aaagaaaatg ctgatatagt
     9541 agttagtgga cattgtgatt ttgcgaatga caaagctatg gtgaaaaagc aacatccttt
     9601 gagtgggaat gtatatgata cgcctaatag tattaaaaat gtacgaaaaa atttatttgg
     9661 acatagtcca aaagatcaag aagtagaggc cttcccgatg tcagtatgta tgtctttgta
     9721 taataaaaat ctgctagata aatatagact aagatttgaa aaaattttat ctgaagatac
     9781 aatttttaac ttgaatgcat atgatgtcgc aaataaaatt gcttttacag aatatacaga
     9841 ttattgttat agaaaagaag aacaagagtc tattacaaaa agtttatctc caaataagca
     9901 gacggaattt aaagaatttt taataactct aaaagctata gcatcgaaag aagatttaga
     9961 atgtcaaatc agagcaaaac gtatggcaat tgattattgt aggctttatg tgggcttagt
    10021 tgatgatgca aatttgggaa ttaaagagaa aagaaagtat ataaaaaaat ttattgaaga
    10081 tgagggatta attcaatgtt ggaagggata tcctacaaat acgctaccat ttcaacaaaa
    10141 attttttcat aaaatgcttg aatgtaagtg ttatacaata ttgttgttgt taagtagaat
    10201 tagaaagata attaaaaata tgaccagaaa aaaattgttt ataatattat aaactaaaag
    10261 tatatcaaat aatagaaatt aacaagttac aaaaaaatat tataaagaat aatattttct
    10321 aaaaagtaaa ctatagtaat gtggattatc accttccaac agtatataaa atgagcgtaa
    10381 aaacggctat ttttgcctta acaacgattt ttttgtatac aaatacgata tgctcattaa
    10441 ttattggaat ttgtagtgct gtttaaatta atgaaggtgt aattatatga gtgatcattt
    10501 tgaaaaagtt tgttgagaag atgtgcttct agtcatgtga tgggagaaaa taatttaact
    10561 gttagagtga atagttaaat tattctggtt atatgaaaaa agtagtttct aaaataatct
    10621 tgcagggatt aacttaaaga aattgagaga atagtttctt tgaaatttct atatacaatg
    10681 agaagctgaa atttactttt atttgatgac tggaaataga aattaaatat attgtattgt
    10741 tacattaaag aaaacttgta tggaaaaatc agtacaatac cattatttaa tgttcttcac
    10801 ttaaagcgca aaaaatatca gtttattaaa ctcgtatata gaacaaatat atgtagacgg
    10861 ggcgaaacga tgagtgtaaa aaaaatgcta tttatggtaa gtacaataat tgtattaaca
    10921 tcccgatttt ggggaattaa tactagttta tctatgcgat attttattat ggcaatatgg
    10981 gatttatatt ttatgttaag ctatttttct tatcgtgaga aaacatataa aaaatacgtt
    11041 atcaagaata acttttggtt gactataatg ccgtttgtga tattttctat ctatactcta
    11101 ataatatggg gaattggttc aaatgcagaa tttgaaaatt ttataaagtt atgttccacg
    11161 ttgctttatt taattcttgc atatggttac gcatgtacag cattttactt atttaaggca
    11221 gaaggaattg attttatttt ttggtcaggt gttatttcat atatgagtgg ttcagttttt
    11281 tatttaattg tatcttatgg tttaaatggt ttaatttctt atataaaaag tcttataaca
    11341 ggtgaaacaa atactgcaaa ttttgctatg gaggtacatg atttaacttt tgcgatgggg
    11401 gtttttttcc tttattacgt attttttgaa aataaaaata caaaaaaaca taagataaaa
    11461 attatcgtat ctttaatttt aattttcatg ggattgaaga gaattgaact gttagcttta
    11521 agtggtgcaa ttctagtgta ttatgtttta ttaaagtggg gaaaaaagat tcaaatccgt
    11581 gcattagtgt gtactatttg tgcaactgtt atttcgttag tgtttattta cattatagat
    11641 acaggaattt tggcattgct tatgtcaaat ttgggcatta cagatgacgg tgcaaggctt
    11701 tcattttatc tatattcatc taaattttat gaacttggtt taggatatgt aggaacagga
    11761 tggacatggt tttcaaaata cttttttaat ctttatttat cgcactttag gttaaatggt
    11821 ggaagaattg cagcaagcct acacagtgat atattaacta tttatataga agttggatgt
    11881 atagttttta ttatatggat gttttggtta ttttttgtga aaccgataag actaactaaa
    11941 aaatttggat tgaaaacagg ggagtgtgta ttattattaa ctatatatat gtttatatta
    12001 tatttgacgg ataatacgct cacttatcca gacactcaaa tgatttttct cttagtacct
    12061 atgatagttt cgtgggttga taatcaacaa cgaatatcgg aatagatagc taaagtatat
    12121 tttaattatg aatttataat tagttgtaaa agttaatatt taacggacag gaagtagggt
    12181 gaaaaaattt gaaaaaaatt ttagtgttag caaatacata ttatcaaata atagtagcaa
    12241 tacaaatgaa aaatacaatt ttttccgatg agtatgttga aattattatt tctgatcatt
    12301 ctaaaaacac taaagaaatt gcgaaaaaaa tagaagaaaa aaaagtgttt gatggctgtc
    12361 attatttaga aagcaaatct tttgatgata atattgggta ttttcaaaag attaaggact
    12421 gtcttcagat ttcttgtggt aaatcaaatc gatatgccta ttatttgaaa aactgtaaaa
    12481 gtgaatattt tgatgaattt atttgttttt caatgggaga ggtttggttg gaaggaatat
    12541 atgcttgctt atatcgaaat aataaaaatg tacaggtatc aattttagaa gaaggtattt
    12601 taagctatgg agttgacatt attccaacag caagaacaaa agctatcgca gtactcaatt
    12661 acttaaaaaa aaggactcca ctatataaca aaagctataa tttttattgt ttttttccta
    12721 atatatatac aggaagagag aatacaatac aaattccatt agttacagct gggtcaagaa
    12781 ccgcattgca actttcggat atttttaacc ctatgataga tatttataaa gaaaaaaaat
    12841 atatattttt tacgtctgtt tatgattttg agggcggaga acctgtcgga gaatatgaat
    12901 tagtatgcaa aatagcagac ttagtaggaa aagataatct aattgttaag acgcatccta
    12961 gagatgtaag aacaatttat aaagataatg gatttatcga ggataaaaat tcatcaatac
    13021 cttgggaggc gattcaattg tcaggcgatt ttaacgataa aatatttatg acaattaatt
    13081 caggaagtgt tatttcaggt agtacaatgt ctgcaaaacc tgtacgcacg tattatatgt
    13141 ataaattgtg taacatagaa ggaaatccgt cttgcaaaaa gaatgcgtat gatattgagc
    13201 gattgcttaa gcaaaaaacg atgaaaaaca ttttaaagaa tgtgaaaata gcagaaagct
    13261 tagatgatat actatgagca aaactaaata tttaattaaa aatatgggaa tattaacaat
    13321 aagtaatttt tcttcaaaaa ttttagtttt tttgttagtc cctttatata caagtgtatt
    13381 atccacaaca gaatatggca catatgattt gacaatttca acagccacat tattgtttcc
    13441 aattttaacc ttaaacattg tagatgctgt gatgcgcttt ttaatggata aagataaaaa
    13501 taaaaaaaat atagcatcta tagcgattaa agttatattt ttaagcgtga tagtctttgg
    13561 atccgtaatg tatttgttaa atattacaaa attatggcaa gatgtttatg gattagaaaa
    13621 atatattttt ttgtattata tatcctatgt atggaatcaa tttctaatac aatttgctaa
    13681 agggttagaa aaagttaaag ctatgggcat agcaggtatt atcagcacaa ttgtaacaat
    13741 aattgccaat attttcttct tgcttattct gaaatgggga ttaattggtt ttttcattgc
    13801 aaatatttta tcacagatta tttcagctac ttatctgtca gtattagtga gaatatggga
    13861 atatatcgat tttgaggcta aagatcctca gctaaagaga gaaatgctaa attactcgat
    13921 tccattaatt gcaactgttg ttggatggtg gattaatagt acagctgata ggtatgtggt
    13981 ttcctttata gtaggtgtct cggcgaatgg cttattgtct gtggcatata aaatacctca
    14041 aataattaat actttgcagg gaatatttat acaggcctgg caaatttctg caattaaaga
    14101 atatggagat aatgagactg caaaatttta tggaaataca tttgttatga ttaatttgtt
    14161 aatgtgcata acctgttcaa gtcttatcat attaactaga ccgttttcac atattcttta
    14221 tgcgaaagac ttttatgaag cgtggaaata tgttccattt ttattaatag ctaatgtttt
    14281 aaattgtgca tcaggtctgt taggacctat cttatcagca aaaaaagatt ctaaatccat
    14341 gatgtggtct gcaatcatag gggcattagt taatgtgatt atgagtgtag tccttgttta
    14401 taagataggg gttcaaggtg ccacaatagc cactgttata tgttcatatg taatatatgt
    14461 tgttcgtaaa cgagctgtgg gaaaagatat ttttataaag cattatcata ttgttgtgga
    14521 atcttgggga ttattgtttg ttcaggcaat catagaaata tattttaaaa attatgtaat
    14581 agaaattata attatgatta ttttaattat tataaatatc agttatatta agcagttgtg
    14641 ttatagagga agaaacatta tttcaatgat taagagtcga gagcacaaac ggtagacgat
    14701 gttattttta taaaaagata taatttatta atggtcgata attatgaaag gaaaaatatt
    14761 cttaaaaaaa agagtatagg atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta
    14821 aataacaaaa gattgccaca aaaaaacacg aaagctttta ccaatggaaa accattgtgt
    14881 tattatatac tttcaacact tttaaaagta aaacagattg ataaagtgta tgtctattgt
    14941 agtaacccag atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt
    15001 tccttagatc aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta
    15061 gaggctgatg tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt
    15121 gaaaaaggac tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa
    15181 ttgcaagatt tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct
    15241 agaacacagg atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc
    15301 aatgtgatga ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt
    15361 gagattgagt cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat
    15421 aatcatctta ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag
    15481 attttagatt gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat
    15541 aatacaaaaa aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg
    15601 tttctcacaa ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa
    15661 ttagcacaga taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat
    15721 ggcgaatata atatagagga tcttccagat tatgatggtt catctattga tggaattagg
    15781 attgcgtttc ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa
    15841 aaaggctact tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt
    15901 ttagcagtaa taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt
    15961 tttggaatga tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta
    16021 gaaaaagata tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat
    16081 gcacaaaaac ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac
    16141 ggaatgggaa gaggagcagg taatcttaac acagaactat ttgtagaata tttgaatgat
    16201 aatgcaggaa aaaaatatca attaaaacca ttattatata taattgatga aatactaaat
    16261 agtttctatc agaagagtta ttggggatat tcattaccaa actatatttc agcagttcat
    16321 aatgctcatc ctaattatgc aagctatctg tcatataaga atactttaac aattgaagca
    16381 atgaatgaaa tattcgaaat gatggatgaa gaaaaaaagg tatcttatga taaacaatat
    16441 atagaagatt tatactacaa atatatggaa actgggaaaa tacaggagga atatatggaa
    16501 gaactgagaa aatttctctc agacaaaaaa attctactga ttgcaccagg aaaaagttct
    16561 gtagatgagg tagaaagaat aaaagagttt gttaccaaag aagaagtcgt aataattagt
    16621 gtaaattttg aatattctca tatagatata gattacgtat ttttaagtaa tttaagacgt
    16681 tttcatgaat taccgaaaga aagcagaact aaatgtattg ttacatcaaa tatacaagca
    16741 tcggatgtat atttacggac gagttataaa aagttgttat ctgaaaggga agttgtaaga
    16801 gacaatgcag gattaatggc aattagattt ttagccgata tgaatgtgga tgaaatattc
    16861 ttagctggat ttgatggata ttcacataat gaagatgaga attatggtga gcaatccatg
    16921 gaaattataa caaaatttgc attattagat gcaatgaacg tgcaaatgag agatgaattg
    16981 tcagaattag ctaaaaaagt aaaggtgaca tttttaacgg atccacgttg ggttcgaatt
    17041 gataagtagt agtaataatg tttttaaaat tttgataaag agatagtgag agaataaaaa
    17101 tgggtaaagt cttaaataaa aaagaagaaa aatatttaag ggccgatggg gcgagatgaa
    17161 actctaattt tgaactttta agaatcattt ctatattgtg tattgttgct taccatgcta
    17221 atttgcgttt aaattataat gcagacggaa ttatagtaca tggttcatta actgctaacc
    17281 agattttctt gaattatatt ggtatttggg gtcagctagg agttaattgt tttatattta
    17341 tttcggcata ttttttaagt gagaaaagaa acattcactt tgaaaatgtt ttgggaattg
    17401 tattagaatg tattttttat agtgtattag tttatggaat tctttgtgca tgtggaatag
    17461 aaacattttc attgaacagc tttgtaaaag aaatattatc atttgctcta ggtagatatt
    17521 ggtttattac atcctattta gcattgtata ttctaagtcc gtatttaaat atcatgctta
    17581 acaatctgga aaaacttcag ttgcaaaaaa taagtatgtt attgtttata ggtgtatgct
    17641 gttatcgtat gttatttaaa acatctttgt ttggggattg tgctgtttta attgcactat
    17701 atgtcatatg ttatacactg aaaaaaacga taataatatt tttgagagac acagaatcat
    17761 aggaacaatt gtatgtataa cagttatacc acttctcata tccataggaa tacttttcaa
    17821 atttatacca aatgcaatgg ttaaccagat ggttcatatt ggttctccat tgatggttat
    17881 agcaagtata tttttatttt atgtatttaa aaatttttca ttttacagta agtatataaa
    17941 taaacttgcg agaaccagtt taggggtata tattttacat gaaaatcatt atttcattga
    18001 tggtatgaag tctagaggct taatatggga tgggatattt ggattaggca aaaggttttt
    18061 agatagggat ttttggattt ggtttataat tgcagttgta gttgtgtgca taatttgcat
    18121 ggctatcgat gttgtaagag aaaaatttga aagacagatt tttaaaatta agagtgtaaa
    18181 gaacctgtgt aaacttatga atcatgttat aaatatatac ccataattaa taaatgagga
    18241 gaaaagaagt gaaagttata tcattcggag aattattatt gagacttgca gcacccggtt
    18301 attcaaagtt atttcaaaga gatagtttgg agactacatt ctgcggaggg gaagctaatg
    18361 tagcggtatc cttagcaata tttggatatc aatcagaatt tataacaaag cttccggatt
    18421 cagatgttgg atatgcggca gctaatgctt tgcgttattt tggagtaaat gttgataata
    18481 ttatatttgg agagggacgc atgggactgt attatttaga aaaaggagca gctcagagac
    18541 catctaaagt aatttatgac agaaaatttt ctgcaatttc gttagctgat agtgatgaat
    18601 ttaagtggaa ggagatccta aacgatgcaa attggtttca ttggacaggg ataaatccag
    18661 cattgtctga gaatctcttt gagacgtcct tagatgcatg tcaggctgcc aaaaaaagag
    18721 gtataacaat ttcttgtgat ttaaattatc gatccaagct ttggacatca gaggaagcaa
    18781 aagaaaagat ggaacagata ctaccattgg ttgatatttg tatttgtaat gaagaagatg
    18841 cagaaaaagt gctaggaatt aaagctgagc atacaaatag gttctataat attttgtgtt
    18901 ggtggaaaat cgaaagcgat tttctactaa cacttttttc ttcgtttcaa
//
LOCUS       DANKEF010000051        17167 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000051 DANKEF010000000
VERSION     DANKEF010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17167
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.051"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(37..846)
                     /locus_tag="PWZ48_002068"
     CDS             complement(37..846)
                     /locus_tag="PWZ48_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2261717.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
     gene            1139..2575
                     /locus_tag="PWZ48_002069"
     CDS             1139..2575
                     /locus_tag="PWZ48_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HDL2261718.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            2745..3941
                     /locus_tag="PWZ48_002070"
     CDS             2745..3941
                     /locus_tag="PWZ48_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HDL2261719.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(4003..4188)
                     /locus_tag="PWZ48_002071"
     CDS             complement(4003..4188)
                     /locus_tag="PWZ48_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HDL2261720.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            4419..7733
                     /gene="dnaE"
                     /locus_tag="PWZ48_002072"
     CDS             4419..7733
                     /gene="dnaE"
                     /locus_tag="PWZ48_002072"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDL2261721.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLGPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            7901..8863
                     /gene="pfkA"
                     /locus_tag="PWZ48_002073"
     CDS             7901..8863
                     /gene="pfkA"
                     /locus_tag="PWZ48_002073"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDL2261722.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            8937..10721
                     /gene="pyk"
                     /locus_tag="PWZ48_002074"
     CDS             8937..10721
                     /gene="pyk"
                     /locus_tag="PWZ48_002074"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDL2261723.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(10756..11658)
                     /locus_tag="PWZ48_002075"
     CDS             complement(10756..11658)
                     /locus_tag="PWZ48_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2261724.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            11844..12413
                     /locus_tag="PWZ48_002076"
     CDS             11844..12413
                     /locus_tag="PWZ48_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HDL2261725.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            12471..12650
                     /gene="rpmF"
                     /locus_tag="PWZ48_002077"
     CDS             12471..12650
                     /gene="rpmF"
                     /locus_tag="PWZ48_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDL2261726.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            12820..14241
                     /gene="gndA"
                     /locus_tag="PWZ48_002078"
     CDS             12820..14241
                     /gene="gndA"
                     /locus_tag="PWZ48_002078"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDL2261727.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDEAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
     gene            14434..15120
                     /locus_tag="PWZ48_002079"
     CDS             14434..15120
                     /locus_tag="PWZ48_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2261728.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            15117..16622
                     /locus_tag="PWZ48_002080"
     CDS             15117..16622
                     /locus_tag="PWZ48_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2261729.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
BASE COUNT         5550 a         2983 c         3596 g         5038 t
ORIGIN      
        1 tgtcacaacc tcttttttgt ggaatctaat tctctattat agtttaagta gtttttccag
       61 ataaacagct aaaccgtctt cctgattgct caaaggagtg acatcatttg caatgccttt
      121 gagttggtca gtaccgttag ccatagctac tccccaacct gcataatcca acaattccaa
      181 atcattatgc tcgtcaccaa aggcaatgac gtctttttga tcgatagaca ggtagttagc
      241 aatctcttgc acgccttttg ctttttggat tccttttgaa acaatctcta agatggcagt
      301 aggtccaccc catgtccgaa catcgacata actgccaaat tgacgtgtta attcagcaga
      361 tacagctcct gcaaaacgtt tgtctgttcg gacaagcaaa gatgtaggat ttgtcgtcaa
      421 atttttagga gaaagcaaat tcttctctcc tggacgagaa gaagcaaaaa ttttttcatc
      481 aaagaaatca aaactgtcaa taaaaaaagt atcacgattt tcagcagcga taaaatctaa
      541 atttagctgc tttttttgcg ataagatttc aaaagcgatt tcacgattga tcaacgtttc
      601 tctttcacca tcccagtgct gattagggat gtgtactaat gcgccgttaa aattaaccat
      661 cggtgttgtt aattctaatt gttggtaaaa ctgatggctc attcggaatg ggcgtcctgt
      721 agcaatactg acatgatgac cggcattgat tgcttttttt agagtctctt ttgttttaga
      781 tgtaatcatc gaatcttgat tgagggtagt tccgtccaga tcgatggcaa ttaatttttt
      841 attcaataag ggtccttcct tcctcaacta gcatttgttt tagtctagca tattttaggt
      901 aattaacaag agcaatcagc tattcctaac aaaaactaca gttatggcta tataaatagc
      961 taaacataag ctaaacacga acgttaagta ttattttcgc tttttttgtt aaaaaaacgt
     1021 tgaaaagcga atgaaacgtc tatataattt gaaattgttc gatggatttg ccctatttta
     1081 cttatttttt attattatag ttttccatca gaaaaaaatt cagggagtgg aactttttat
     1141 ggaaaagttt ttcggtttaa agaaaaacaa tacaaatgta tcaactgaaa taatggcggg
     1201 agtaacgaca ttctttgcca tgagttatat cttattcgta aatccgacga ttttgtcagc
     1261 atcaggtatg cccttccaag cagtatttct agcgacaatc attgcatcga ttattggtac
     1321 gctggtaatg ggattgttcg caaatgtacc atatgctcag gcacctggta tgggattgaa
     1381 cgcatttttc acttttacgg tcgtttttgg gcttggttat tcttggcaac aagccttggc
     1441 aatggtgttt atttgcggat taatcaatat ttttattact gtcacaaata ttcgaaaaat
     1501 gattattcgg gcgattcccg aaagtcttca acatgcgatt ggtggcggga tcggtatttt
     1561 cgttgcgtat gtcggaatca aaaatgcagg attcttatct ttttcagcag accagtcagc
     1621 tatttcgagc tctgtcgttg aaggcggaaa agcgacaaat gttacgataa atggtggaat
     1681 cgtccctgcg ttagccaact ttgacaacgc tcctatctta ttggctgtga ttggtttggt
     1741 actgacagcc attttagttg taaaaaatgt ccgtggggct attcttatcg gtatcgtagc
     1801 gaccacagtc cttggtattt ttatgggtgt tgttgattta agttccattg attggcaaac
     1861 gaattcgtta ggtaattcat ttaaagaatt aggaacaact ttcggtgcag catttggttc
     1921 tgaaggcatg caatctttat tcagtgattc ttccaagatt cctcaagtat tgatgacgat
     1981 catcgcattc agtttatctg atactttcga tacgatcgga acatttatcg gtacaggacg
     2041 ccgcacaggt attttctcaa aagaagacga gatcgcgttg gaagatggtc gtgggttcaa
     2101 aacaaaaatg gataaagcac tatttgccga tgcgatcgct acttctgtgg gggctatctt
     2161 tggtacatcg aatacaacaa cttatgtcga atctgcagct gggatcgggg ccggtggacg
     2221 tactggtttg acttcagtag tagtggcagt gctattcgca ttaagtagtt tattctctcc
     2281 actgatcgcc attgtaccaa atcaagcaac tgcgccagca ttgattttag ttggagtaat
     2341 gatgatggct tcttttgcag acatcaaatg gctagatatg gaagaagctt taccggcctt
     2401 ctttgcatcg atctttatgg gattgtgcta tagcatttcc tatgggattg cagcaggatt
     2461 tattttctat acaattgtga aagtaatcaa aggaaaatac aatcaagttt ctgttgcttt
     2521 gtggatcgtt gatattttat tcattatcaa cttcatcatt ctagcaacaa tttaactaaa
     2581 gagaagggat tggaacagta tttgttctga tctctttttg ttttacataa aaagaacgtt
     2641 acatataaaa tgacctttgc gggatttttc tctttttact ggcgagatcg ttgcttgttt
     2701 attcgaaaat aggtatgata aaagagacga aagagggatg atagatggag attaattgga
     2761 gaaaaaattt agtgattgcg tggattggct gttttttcac tggtgcaagt attagtttag
     2821 ttatgccatt cataccagtt tatgttgagc aactaggaac accaaaagat caaatcgaat
     2881 tgttttcggg actagcaatc tctgtgactg cttttgcatc tgccgtggtt gcaccaatct
     2941 ggggaaatct agctgatcga aaaggccgaa aactcatgat gatccgtgca gcagcaggga
     3001 tgacattaac aatgggatct ttggcttttg taccaaacgc gtattggctt ttgatcatgc
     3061 ggttttggaa tgggattttg tctggatata ttccaaatgc cacagcgatg attgcttcac
     3121 aggcacctag agaaaagagc ggatgggcgt taggaacatt gtctaccgga gcgattgctg
     3181 gaaatctgat cggaccttca atgggcggtg cactggctca gtggtttggt atggaaaatg
     3241 tattcttgat cacaggggct ttgttgatga tcacaacggt attgaccatt ttcttagtca
     3301 aagaagattt ccatccaatc gagaaaaaag atctgataag caccaaagaa attttttcaa
     3361 aaatggatca tctctctatt ttgatcggtc tcttcattac gaccttgatt ttacagatag
     3421 gaatcactac gatcagccct attttgacct tatatattcg tgaattgagc ggaagtacag
     3481 aaaatatcct ttttgtcagc ggcttgattg tttcgatagc tggtgtttcc gctgtctttt
     3541 cttcaccgaa actaggaaaa ctaggggata agattgggaa tcaaaaagtt ctaattggag
     3601 gactcatttt atcattttgc tgttatttac ctatggcatt cgtcactaca ccgctccaat
     3661 taggtatact tcgctttatt ttaggctttt caactggcgc tttgatgcct tctgtcaata
     3721 cactgatcag taagattacg cctcaagaag gagtcagccg aatctatagc tacaatcaga
     3781 tgtttagcaa ctttggacaa gttttaggtc ccatgctagg atcgacagtc gcgcatgctt
     3841 ataattacag tgctgttttt attgtgacaa gtttgtttgt tctgtcaaat attcttcttt
     3901 ctttattcaa ctttcgtaaa gttcttcgca gcagattata atggtggtga ggagtagaaa
     3961 ataaaaaaga gcttgagaaa tctcaagctc ttggctgttt ttttacgaat gggcagaggt
     4021 ttcttttctt ggcgcaactg caccaatcaa aaggataatg acacccgcaa tgaatgaaat
     4081 gattgttgtc aaagtaaaat catatgttcc gcttgttaat gcagcaccaa tataaccgac
     4141 tacttgacct agaacgacag cccagaataa tgtaacgata tatcgcataa agaacacctc
     4201 ttttctatct taattttacc agattttctt tgaatgtaaa gaaacgagac caaatctcaa
     4261 taaaatttga gttcttgcga aagaactcat tcctacagat tacaagcaag caaatgaagt
     4321 ggaataatca acttcagcgc ctgctatctt aatgaaagta aaaggatcaa accttttaac
     4381 gctagtgaat tttaacgaaa aaaaaggcgg aatgcaagat gtttttccca caactttata
     4441 caattacttc ttattctctt ttacaaagta cgatacgtat tcgagaatat gtacaagaag
     4501 caaaaaaaca aggctacact tcattagcca taacggataa agatgtctta tacggtgcac
     4561 ctgaattcta tcgtatttgt gtagaagaag gagtacagcc aatcattgga ttacatctta
     4621 gttatactta tgaagaaaaa aattatggct tgcttgcgta cgcaaaagat gaaataggtt
     4681 atcagcagct gatgaaactt tccagccgaa aaatgatcca agaaaaattg gaacttgcag
     4741 aaatgacaga aaccgaccat ctagcgtttg ttgttccggc aaatcattct gtttctgagg
     4801 ctttcaataa agaggataac tttttcggtc attggcagtt tttacttgaa aatgttttta
     4861 ctagaaattt ttatatcggc ttgacagaaa acacgcctga tcaattgcct gaatggtatc
     4921 agtatgtgat aaaagaaaat tattcgcttt gtgcattaca tcccatcgct agtttacaac
     4981 aagaagaact ttttgcactt gaagtgatga aacatatcaa agagggaagc caaatacctt
     5041 tagaaaattt atctgcatct ttaccactaa cagcaaatgt ctatcttctt tcagaacaag
     5101 aaatgatcaa acgattcgaa aaagcgggtc tttcccaagc tgtaataggc gcccagcgtt
     5161 tagctgaatc gatttcattt gaattccagt tccatcagaa attacttccg cattatccag
     5221 ttcctaatgg aaaacaagct ggagaatatt taagagagtt atgctattcg caagtggaac
     5281 gccgaatcac tgactataca aaggaatacc aagaacgttt ggactacgag ttatctgtca
     5341 tccatcaaat gggattcgat gattatttct tgatcgtatg ggatgtaatg gcttttgctc
     5401 atagaaacaa aatcgttacc ggtgcaggtc gtggttctgc tgcgggttca ttagtagcct
     5461 atgtgttaga aatcacagat gtggatccat tagaatatga tttgctgttc gaacgctttt
     5521 taaaccccga acgctttacg atgccagata tcgatttgga tattcctgac aatcgacgtg
     5581 aagaagtatt gaattatgtt cgagaaaagt atggacaata tcatatggca caaatcgcaa
     5641 catttggcac aatggcagca aaaatggttc ttagagatgc agctagagtg tttggtttat
     5701 cgcaaagtga agcaaaccgc tggtcaaaag caatacccaa tcaattgaaa atcacattga
     5761 cccaagctta taaaacttcg catcaattag tagaattagt tcaggcaagc gaaaaaaacc
     5821 aacttttatt caaaactgca gtgattttag aaggattgcc aagacatgtt tcgactcacg
     5881 cagcaggtgt agttatcagc gataagaatc tacttgaact agttccgctc caacgcggtt
     5941 ctgatgaagc ttttctgacc cagtatacga tgaatgaggt agaagcaatc ggtcttttga
     6001 aaatggactt tttaggacta cgcaacttat cgattattga ttttactttg aaagctgtta
     6061 agcgagtaga agggtatgag atccgattaa aagagatccc gctaaatgat gcaaaaacgc
     6121 tgctcttgtt tcaacgaggc gaaacctcag gagtatttca atttgaatct gctggtatcc
     6181 ggaacgtttt acgaagatta ggtccagaaa acatcgaaga tattgccgca gtcaatgcat
     6241 tgtatcgacc aggccctatg caaaatatag ataccttcat tgccaggaag aaaggaaaag
     6301 aagccattcg ctacccagat gattcactga tcccgatttt gaaaaacaca tatggagtga
     6361 tcgtctatca ggaacaaatc atgcagatcg cctcgactat ggctggattt acattagggc
     6421 agtcagatat tctgcgacgg gcagtgagta aaaagaaaaa agacgtatta gatgaagaaa
     6481 agtcgcattt tgtatcagga gcattacaac agggatattc ggaaaaaaca gcagcgaccg
     6541 tatatgaata tatcgaaaaa tttgcaaact atggattcaa tcggtcacat gcatttgctt
     6601 attcattcgt gggattccaa atggcgtatc taaaagccca tcatccaggt gctttcttta
     6661 ccagcctgat gaattctgtt cggcataaca catcaaaatt gaaagaatat attgcagaag
     6721 cacgtaaaaa taaactcaaa ctttcctcgc cgtccattaa tcaaagtaat tccagttttg
     6781 agttggtcaa taaggaagaa atcagattcg gactgacagc gataaaaggt attcggcgtg
     6841 attttgtaga agatatatta aacgaaagaa aacaaaatgg cccgttccag tcagtggatc
     6901 aattcctcat acggatcgat aagcgttggc tgaaattaga gttactgcaa tcactgacag
     6961 cagtaggcgt ttttgatgaa ttatctccta acaggaaaca attgatgctt gatctcgaag
     7021 gaaaaatcca aaatatcgtt tacagtggtg gaagtcttga cttgctggga atcatggcat
     7081 taaaagaaga agaagtcgct gactatacac tagaagaacg cttgaagctg gaagaggagt
     7141 acttaggtat ttatttgtca ggtcatcctg cagaagggtt tgatcgtctt cgtttagcta
     7201 aacaagttca gctgattaca gatatcgtcc ctaatcaacc agtcaattta ttagtgatga
     7261 tcaaggaagt tagagaaatt cggacaaaaa aaggagaact gatggccttt gttgaaggaa
     7321 ccgacagtag tggagaaatt tctgtcacag tttttccaac tctttatcgt aaatatcgta
     7381 atttgtttgc cgttgatcga gtgatctttg ttcaagggcg gagcgaaatc agcaaatata
     7441 atcaggaact gcaactaatt ggagaaagct ttgctgatcc taaccagtta gaagcccagt
     7501 atccttcaca aacttgctat ttgcgtatta cagaagaacg ggaagatccc gagataatga
     7561 aacaattaca agtgattttt cagaaacacc caggatatat accagtagtt atgtatcacg
     7621 aaaaaacgca acgaaaagta gttctgtcag atgcttattg ggtggatgac agcaagagta
     7681 taaaggggca acttatttat ctgcttggcg aagataatgt ggttttcaaa taacttacgg
     7741 taaaaaatac tacttgcttt catttttttt cattttttta tccgtaaatc aagacaagtg
     7801 agaaaaataa tgttagaatg ttagtcgtgg ttaacgcaaa aatggttgta aacctaagtc
     7861 atacttagga taaagtattt taatgaggtg aatgtttatt atgaaacgca tcggaatttt
     7921 gaccagtggc ggagacgcac ctggaatgaa tgcagctgtt cgtgccgttg ttcgtaaagg
     7981 tatttacgaa ggactggaag tatacgggat caattatggt tttgccggtt tagttgctgg
     8041 agatattcgt cgtcttgatg tggcagatgt aggtgacaag atccaacgtg gtggtacatt
     8101 cttgtattct gcacgttatc cagaatttgc aactgaagaa ggtcaattaa aaggaatcga
     8161 acaattgaaa aaattcggaa tcgaaggtct agtggttatc ggaggagacg gctcttatca
     8221 tggcgcaatg gctttgacaa aacgcggttt cccagcagta ggtattcctg gaacaatcga
     8281 taatgatatt cctgggactg atttcacaat cggttttgat acagcaatca atacagtact
     8341 agaatcaatt gaccgtattc gtgacacagc gacttctcat gttcgtacat tcgttatcga
     8401 agtaatggga cgcaatgcag gagacatcgc tttatggtca ggtgtggctg gcggagcgga
     8461 tgaaatcatc atcccagaac atgattttga tatggcttca gttgctaaaa aaatccaaga
     8521 aggccgcgat cgcgggaaga aacattgctt gatcatcttg gctgaaggtg taatgggcgg
     8581 aaatgaattc gctgagaaac tttcagaatt tggcgactat catacacgtg tatctatctt
     8641 gggacacgtt gtccgtggtg gagcaccaag cgcacgcgac cgcgtgttgg caagtaaatt
     8701 cggtgcttac gctgtcgaat tgcttcaaga aggaaaaggt ggattatgtg tcggtatcca
     8761 tgacaacgaa atcgttgcgt ctgacatcat tgatacactt gaaaacaaca aacataaacc
     8821 agacttatca ctatatgatc taaataattc actttcattt taagagtgaa agaaaccaag
     8881 ctatttaagc tgtgtacagc tttaaataaa caaaattgaa tgggagagtt ttttaaatga
     8941 aaaaaaccaa aatcgttagt acattaggac cagcaagtaa ctcagtagaa attatttctc
     9001 aattgattga atctggggca aatgtcttcc gctttaactt ttctcatggt gaccatgaag
     9061 aacaattatc acgtatgact atggtccgtg aagcagttaa aaagacagga aaagatgtcg
     9121 gtattctttt agatacaaaa ggagctgaaa tccgtacaac tgttcaaggc acaacagaag
     9181 ctgattttgg tcgtgcagga tacatcaaat ttgaagtagg agatcaaaca cgcatctcta
     9241 tggatcctga acatgtaggt tcaaaagaaa aaatcgcggt tacttaccca ggattatttg
     9301 acgatgttca cgttggcgga cacattttat ttgatgatgg tttgatcgat atgcaaatca
     9361 ttgaaaaaga cgaagcaaac cgtgaattag tcgtagaagt taaaaatgca ggtatgctag
     9421 gatcacgtaa aggggtaaat gcaccaggcg tttctattag tttgccaggt attactccaa
     9481 aagatgctga cgatattcgt tttggtttag acaacgatat cgactttatc gctgcaagtt
     9541 ttgtacgtaa agcacaagac gttctagata tccgcgaaat cttagaagaa aaagacatga
     9601 ctcatgttca aatcttccct aaaatcgaat ctcaagaagg tatcgacaac attgacgaaa
     9661 tcatcaaagt ttctgacggt gttatgattg cccgcggaga tatgggtgtt gaaatcccag
     9721 ctgaactagt accaatggtt caaaaacgca tcatcaaaaa atgtaatgct gctggtatcc
     9781 cagttatcac tgctacacaa atgttagaat caatgcaaga aaacccacgt ccaacacgtg
     9841 cggaagcttc tgacgttgct aacgctgtat ttgatggcac agatgcaaca atgctatctg
     9901 gtgaatctgc aaacggggac tacccagttg aagctgtttc aacaatggca cgtatcgacc
     9961 aagaagctga aaatgcatta tcagaattag gtacattcca attgaacgaa ttcgataaaa
    10021 cagatgtaac tgaaacaatc ggtttatctg tagctcgtgc tgcaaaaaat ctaggcgtga
    10081 aaacaatcgt tgctgcaact gaatcaggat acactgcaaa aatgatttca aaatatcgtc
    10141 cagatgcaga tatcttggct gttacttttg acgaacgcac aaaacgtggt ttgatgttaa
    10201 actggggcgt ttacccaaca gttgctgaaa aaccatcaac aactgatgaa atgtttgaac
    10261 tagcagctaa aaaagctgtt gaattaggtt tcgcatcaga aggcgattta atcttgatca
    10321 ctgctggtgt accagttggt gaacgcggaa caactaacgt gatgaaaatt caaatgattg
    10381 gttctaaatt attagaagct caaggcgttg gacaaaaatc tgtcgttgca aatgcagtta
    10441 ttgcttcatc tgctgaagaa gctgtaaaaa aagcaaaaga cggaatggtt ctagttgtac
    10501 catcaactga caaagaatat atgcctgcaa tcgaaaaagc agctgctgtt atcgttgaag
    10561 aaggcggatt gacttctcac gctgcagttg taggaattgc acaagatatt cctgtaatcg
    10621 ttggtgctaa aggcgcaaca acaacaatta cagaaggtga attgattacc gttgattcac
    10681 gtcgcggcat cgtttaccgt ggcgaaacaa cagctatcta atttagctta ataaaaaata
    10741 aaaacagctc ttcttttacg aagagctgtt tttttgtgct gtaacgaatt catggataac
    10801 ttggactact tgttcttgaa gaggcgtgac gatctgctgt gttcgttttg ccaatgaaat
    10861 caagaagcga ggctgaactt catctgaaag agggaggcaa actaaatgat cttcaggatg
    10921 gacagcaatc tctgccaaaa aaccgatacc cacattttct cggaccatac tctttaaaat
    10981 cgtcaagtca ttgctttgat aaacaacttt tggatgagta tgtgtttgtt tagaaagctt
    11041 agtgaatgct gcggggtgga cataatgttc gttcaatagt acaaactgtt catctgctat
    11101 atctttgaaa gagacagtat cctgcttgga aagaggatgt gtaggtggca cgacgatcat
    11161 gaaacgtttg tccaaaagta actctgtttc taaatattga tccacgattg gctgtgttga
    11221 gcctaataca gctaaatcga tcctcccttg tcggatcatc ccgtataagt cccgagagcc
    11281 tccatcggta atctcaattt gattcatcaa ttcatgcttg ataaagtaac ttgacagttt
    11341 aggaaagtat tcattgccga taataggagg catgccaaaa cgaagctttt tcttttgcaa
    11401 ctgagtgatt tcagctcttg cagtagaaat ctcttggaga atggtttgtg catggagaaa
    11461 cagctgcttt ccagaaggcg tgacgattaa ttcgtgatgc gatcgatctc ttgtaataag
    11521 tacggtgtca aactcttttt caagacgttg taatgcatat gtaatagtag gttggctcac
    11581 acggaaataa ttagccactt tcgtaaaatt tttttgtttg acgatttgga catagtattc
    11641 gatatctttc gtgttcatga agatcatccg ctcctttaat ggaataatga ttattgtaaa
    11701 tgattctgag gatgatagca aacagaggct ttctaataaa gatttttctg tttttttatg
    11761 aactgcaagg tgtcaagaaa atttcattga caacatgctt tttgccctgt ataattgctt
    11821 ttgttgctta ggagtgatga aaaatgaaat ggtctttatt ggaattgaga aaataccagg
    11881 agacaccgtt aacttttcat gagacgctcg acttgaaagc cgctctcatg aagcgagaca
    11941 atttaatact tgatgttgcg ccagtggaag tcgaaggttt ggtctcagtt gataagaaag
    12001 gctatatcgt tcattacact gtgcagacga ctttgacagt tccttcaaca cgttcactag
    12061 aacctgttga actgcccatg gatttttctg tagacgaagt attcatgaca gaagaacaat
    12121 tccaaagtag agatgaacaa atgtctgcgg atgagatttt gctaattgaa ggtccattat
    12181 tgaatttagc agagtcgatt gaagacaaca tcttattggc gattccaatg cgggtattga
    12241 caaaagaaga ggaagcagca acagaacttc caaaaggcaa tgattgggaa gttgtttccg
    12301 aagaagaata ccagcgcaga aaaatggaag aagccgagaa taaaattgat cctcgtcttg
    12361 caaaactatc tgaacttttt gattctaaat caaaagacga cgataacaag taagattgga
    12421 atatattgtg tctaatttag atacaagact ttacaaggag gtgtagcaaa atggcagtac
    12481 cagctagaag aacatcaaaa gctaaaaaag caaaacgtcg tacacactac aaattgacaa
    12541 tcaaagggtt aaacgaatgt ccaaactgtg gtgaaatgaa aaaaagccat cacgtatgtg
    12601 caaattgtgg ttactatgat ggtaaggacg taatgtcaaa agaagcttaa tacattttaa
    12661 ttagaaaatg taaaacctca aggctaacca tggagccttg aggtttattt ttttgtagtc
    12721 ggtaaataaa aaatatgttg ttctttagtt tgcaatgtag ccgtgttaag ctataatgta
    12781 agagattagg gagaaatccc gtatgtagga ggagttcata tgtcaaaaca acaaatcggc
    12841 gttgtcggta tggccgttat gggtaaaaat ctagccttga acatagaaag ccgcggctat
    12901 tcagtggctt tattcaatcg tacaggggct aaaacaaccg ctgtagtaga agaacaccca
    12961 gataaaaatt tcaaagcaac atatacaatc gaagaatttg tagaatctat cgagaaacca
    13021 cgtcggatct tgctaatggt caaagcagga ccggcaacag atgcaacgat ccaagagttg
    13081 cttcctcacc ttgataaagg cgatattttg attgacggag gaaatacatt ctttaaagat
    13141 acgatgcgtc gtaatgaaga gttagccaac tctggcatca actttatcgg tacaggcgtt
    13201 tccggtggtg aagaaggagc tttaaaagga ccttcaatca tgcctggtgg acaaaaagaa
    13261 gcctatgcgc tagttgcacc aatcttagag caaatttctg caaaagcaga agacgaagca
    13321 ccatgtgtca catatattgg cccaaatgga gccggacatt atgtgaaaat ggtacacaac
    13381 ggtattgagt atggagatat gcaactgatt gctgaatctt atgacttaat gaagaatatt
    13441 ttaaaccttt cagtagaaga aatggctgat atctttaaag aatggaatca aggcgaatta
    13501 gacagctatt tgatagaaat cactgctgac attctgacac gtaaagacga tgaaggaaca
    13561 ggtaaaccag tagtagatgt gatccttgat gcagctggga ataaaggaac aggtaaatgg
    13621 acaagccaaa gcgcgcttga cttaggtgtg ccacttccat tgatcactga atctgtattt
    13681 gcccgctata tttctgctta taaagaagaa cgcgtacaag caagcaagat tttatctcgt
    13741 acgaacgatt ttgagttcac gggagacaaa aaagaattgg tcgaaaaaat ccgagaagcg
    13801 ttgtatttca gtaaaatcat gagttatgcc caaggatttg ctcaattacg tgtcgcttca
    13861 aaagaatttg attgggactt gccatttggt gaaatcgcaa aaatctggcg cgctggatgt
    13921 atcatccgag ctcgattctt gcaaaaaatt acggatgcct atgacaaaaa tcctgagatc
    13981 gaaaatttat tgctagatga ttacttcgtt gaaatcacga aaaaatatca gcaatcagtc
    14041 agagacgtcg ttgccttggc tgtccaagct ggcgtacctg taccgacttt ctcatcagcc
    14101 atcgcttact tcgattcata cagagcagaa cgtctgcctg caaatataat ccaagcccaa
    14161 cgcgattact ttggcgcaca tacctatgaa cgtgtcgaca aagaaggaat cttccattac
    14221 tcatggtacc atgaagaata atagtctttc ggctgttagg aaatgagaaa ataaaaagag
    14281 gacgaaagag ctgaagtcga ttgttatgag tagacttcag ctctttttta aaattgctat
    14341 ccatgataaa atgactatag atttttaagc atattatcgg tataatgata gagtttttaa
    14401 aaagccagta gaatagaaag gataaaataa taaatgagca atattctaat cattgaagat
    14461 gaaaagaact tggcacgatt tgtcgagcta gagttgaagc atgaagggta taacgcagaa
    14521 gtccactaca acggccgaac tggtttagat gcggcattaa ataatgagtg ggatgccata
    14581 ttgttggatc tgatgctccc agaattgaac ggtttagaag tctgcagacg tgtacgccaa
    14641 gtcaaaaata caccaatcat tatgatgact gcaagagatt ctgtgatcga tcgtgtatct
    14701 ggacttgacc acggagcaga tgactacatc gtcaaaccat ttgctatcga agaattatta
    14761 gcacgtcttc gtgcattgtt gcgtcgaatc gatattgaag gcgataaaaa tgtggctaaa
    14821 caaactacta ttacttatcg cgatttgact atcgaaaaag aaaaccgtgt cgtacgtcgc
    14881 ggaaatgaag taattgaact aacaaaaaga gaatacgagt tgctgcttac tttaatggaa
    14941 aacgtgaacg tggtattagc acgtgacgtt ttactaaata aagtgtgggg atacgaaaca
    15001 gaagttgaaa caaacgtagt ggacgtatat attcgttatc ttcgcaacaa aattgacgtt
    15061 cccggggagg aaagctatat ccaaactgta cgcggaacag gttatgtgat gcgctcgtga
    15121 taaagccgaa aaaatcggaa aaaagagaac tgaaagggcc ctccttgaca atcaagtggg
    15181 cctttgcgag ttctttcttc atattcgttg tgtttacaat tttcgctgtc attacctata
    15241 aatcatctat caatctgatt gtggcaaaag aaagaaacaa tgtagagcgt acgatttcag
    15301 aagtagcttc tcgtttggct aactcggatg aagaactgac attagtaaat acgtatcgta
    15361 atctgacgaa agcgacaaat gacggagatg aggtcgtaga tcatacttca ctggtcgaag
    15421 gaaacttaat gaagatcgat tcctttatag ctgagctagg gcagccatca atggatcttt
    15481 atgtttatga tttggacgag aacttgattt ttaaaaccca tgaaaacagt cgaaacctga
    15541 ttcagactac acgtaaagtt ccaacgatcg ttacactaga aggaaaaact ggctttttat
    15601 ctatccagcc tatatattct aaaggtacaa gagaaaagat cggctatgcg caaagcttct
    15661 atgaattatc ttctttctat gatattcgaa ataagctatt attgactctt attgttttag
    15721 aagttgtttc tttgatctta agtagtattt taggtttctt tttatcttct tatttcttaa
    15781 aaccactcaa ggtactcaga gatacaatgg atactatacg taaagatccg caatcagata
    15841 tccacatgcc tgaaatcgac acaaatgatg aattggcaga tttagctgaa atattcaatg
    15901 agatgcttga ccgtatgcgt ttgtatattg aacaacaaga gcaattcgtg gaagacgtat
    15961 ctcatgagtt aaggacacct gttgcaatta ttgaagggca tcttagtctg ctaaatcgtt
    16021 gggggaaaga tgatcctgag atattagaag aatcattaag cgccagcatg caagaaatta
    16081 gccgtatgaa aagcttagtt caagagatgc tagacttatc aagagcagaa caagtagatg
    16141 ttcattatgc gaatgaaaca actaaaggaa aagaagtcac ctatcaagtt tataataatt
    16201 tcaagatatt atatccagac tatgtgatca ctttagatga tgatttgaca aaagaagtga
    16261 ccctgcaaat ttatcgtaat cactttgaac aattaatcat tatcatattg gataacgcag
    16321 taaagtattc aactacacga aaagaagtcc atatctctat ttcttccact ctgaatgaat
    16381 ttgagatcgc gattcaggac tttggcgaag gaatacccaa agaagacctt aataagatct
    16441 tcaatcgttt ttatcgagtg gataaagcaa gagctagaac gaagggtgga aatggtctgg
    16501 ggttgtctat cgctaaacaa ctggttgaaa actataaagg acgtatctta gcagaaagtg
    16561 tagtagggca agggacaatt tttagaatct ttattcctat cgcaaaggca gaagacaagt
    16621 aaagcatgtg ctttacttgt ttttttatat atccaacaaa gaataaggta caaaatacaa
    16681 gcaagttaga aattcaggga tgtcattata aaaaaaacta gcgaacaatt aaatagaata
    16741 acatccgtta attaaacgta agtagctttt agatgaacga aaaaacaatg aaatgctgta
    16801 atattcgtgt ttaaaaataa taaataaaaa tagggaattt tctattgcaa ttttataaag
    16861 agattgctat attattattt gttctaaatg attcctttaa ggtgtcacta aaaattataa
    16921 aaaatagggt tgacgcactt ttgatgtcat gttataataa cgaagtcgtt tggatgaggt
    16981 agctaataaa gcttgatttt atcgaaaaaa acttcgaaaa agttgttgac aaataacaat
    17041 aaagatgata tgataatcaa gtcgctgcta atgagtgaca aagacgttta gaaaaaataa
    17101 acaaagaagt tgttgacatt ctcttctgaa tctgataaga ttaaagagtt gttgaaacaa
    17161 aaagatt
//
LOCUS       DANKEF010000052        17007 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000052 DANKEF010000000
VERSION     DANKEF010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.052"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..126)
                     /locus_tag="PWZ48_002081"
     CDS             complement(<1..126)
                     /locus_tag="PWZ48_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HDL2261730.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="PWZ48_002082"
     CDS             complement(230..541)
                     /locus_tag="PWZ48_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2261731.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="PWZ48_002083"
     CDS             complement(543..740)
                     /locus_tag="PWZ48_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HDL2261732.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="PWZ48_002084"
     CDS             complement(1134..2861)
                     /locus_tag="PWZ48_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HDL2261733.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="PWZ48_002085"
     CDS             complement(2854..4047)
                     /locus_tag="PWZ48_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HDL2261734.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="PWZ48_002086"
     CDS             complement(4234..4878)
                     /locus_tag="PWZ48_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2261735.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="PWZ48_002087"
     CDS             complement(4972..5106)
                     /locus_tag="PWZ48_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HDL2261736.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="PWZ48_002088"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="PWZ48_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HDL2261737.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="PWZ48_002089"
     CDS             complement(7237..7710)
                     /locus_tag="PWZ48_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HDL2261738.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="PWZ48_002090"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="PWZ48_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDL2261739.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="PWZ48_002091"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="PWZ48_002091"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDL2261740.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="PWZ48_002092"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="PWZ48_002092"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDL2261741.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="PWZ48_002093"
     CDS             complement(11757..12896)
                     /locus_tag="PWZ48_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDL2261742.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="PWZ48_002094"
     CDS             complement(13205..13918)
                     /locus_tag="PWZ48_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDL2261743.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="PWZ48_002095"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="PWZ48_002095"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDL2261744.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="PWZ48_002096"
     CDS             15107..16639
                     /locus_tag="PWZ48_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HDL2261745.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="PWZ48_002097"
     CDS             complement(16830..>17007)
                     /locus_tag="PWZ48_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2261746.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5277 a         3494 c         2911 g         5325 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta attcccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagagacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DANKEF010000053        16668 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000053 DANKEF010000000
VERSION     DANKEF010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16668
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.053"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..362)
                     /locus_tag="PWZ48_002098"
     CDS             complement(<1..362)
                     /locus_tag="PWZ48_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2261747.1"
                     /translation="MESYQFQVFKLVPSLERRGNRMKITEKNVNPKFETTESNLKKFD
                     LTVTLSNQLVDSIVEMDAMPQKIYLYAIGHLDPKNPPFNNTVKVSKKALFALMSSSSS
                     SSKHSRLKQVIEKLHQQSV"
     gene            1343..2146
                     /locus_tag="PWZ48_002099"
     CDS             1343..2146
                     /locus_tag="PWZ48_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2261748.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKDLSDKLGNLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
     gene            2731..3186
                     /locus_tag="PWZ48_002100"
     CDS             2731..3186
                     /locus_tag="PWZ48_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261749.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            3305..4363
                     /locus_tag="PWZ48_002101"
     CDS             3305..4363
                     /locus_tag="PWZ48_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HDL2261750.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLDERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVSKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            4405..6354
                     /locus_tag="PWZ48_002102"
     CDS             4405..6354
                     /locus_tag="PWZ48_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2261751.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKSEDKENEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQL
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFK
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
     gene            6357..9998
                     /locus_tag="PWZ48_002103"
     CDS             6357..9998
                     /locus_tag="PWZ48_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261752.1"
                     /translation="MKFKKYLLQNLRIKRWVVRGLVIGGVLAVTLGAPFGMNDPTPDN
                     QDSKQETVINKNVAYAESKPAEQGMWTLYARILMGDKEVNDSAAKTGQMNLLGQGGIS
                     ADFPYSDISKQGNSIAGGNAGTQLASTLATYSYYGYIETVSGNAIASQASSILGSIGR
                     TIGSFIGSISLILSNIAESINELLGKFFMSMNIFSLMGWGEGGSDVSNPVSKALTSLL
                     NNLGLNNELFKAIASLSMLFFTAILGYQGMKLLSKDGVNSKGLSPVLEKWIVRIFVLF
                     IFLPVAGSLLGVYINGIQSAKKDGDIPTNVVSDYIINTRLWASTQNLAPSSDTGGDVP
                     DGKPTSEIKFVDTDYEPSKKSAMIRAINTSSYARQNNDLDSNSVGMALLKEWSSNDTF
                     NINTYAGDVNVAENDAYMGRAPAVWTGYNLGDGKEGSAIKKPTNLNGYIWSANQNVTD
                     DNRKPDGKGTAHNFDATQPWGVANNATFSTQSVALLLQSALDTSGAKFYAYNIAPSGI
                     QGSAKNMSTVKTEWRTASLVGHGAMGKFGSWLAMNAESIVLAVLYLACAWAMFNVNIV
                     KAFKREFIRIWEVVLTGSLASGAGVMAISLGVVTTSLIGLQLPIFLITFIRAFGDTAV
                     GFIPNTLVGDGVANIVRSAMILGVGYLLVFKKGEKVNNLINMLIGLPLEVAFGFDERA
                     KLLFGNRNDAVRVVDGVSKAIKKSQGAKNPYYDGGNSSVKDMVAGGLGAVGAQKMAER
                     LNNMGDSNADAKSIADKVIPETPEGMSFNGGGVGSTINNTSFKDLVRQDQLRNNSQQY
                     IDHLGRMVDENGNFVDENGEILKDSKYGKDAKGNFIDEKGRLVDEDGNLIDTNGKPLE
                     DSEDGNIGMDSEGRLIDEKGRLVDEDGNLVDENGRRLLNGDFGHDSNGRLVDEKGRLV
                     DEDGNLVDEFGNKLGKHSDGYNVNGDLVDEDGNIIETRDALVADRNRELEQQRHTPIA
                     VAIPENEEDEITLDQTHQELRNEMSGEDVSDRVHRELGDGASGESQSRELNSNFDNSA
                     IRNDTVLKDRESLTKETDKEQSDVKKKFIPTPNVNQTDKYETNIGSGMSKDNQSIREP
                     ELTNKNNRTNNSKSTSDNSGKSNQSTKKTSTAKSAVEYLSKTGKNVYTSADKALAKAP
                     KGSLAGITYEAKNEAIKIGKGTAKVAKATATGASKTGKVVKKSTMSVKDYIVKYGNQK
                     STENKSEDE"
     gene            10398..10736
                     /locus_tag="PWZ48_002104"
     CDS             10398..10736
                     /locus_tag="PWZ48_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261753.1"
                     /translation="MFDSIKNLVSGMTPDMKTLVQVVLYFLAFVFSLKPGFTAMKFFG
                     DAKWGDGLKYVGAIIAIFIIPVITTVMLYTMGTKTGNDLNQKANIISALIPVVGIYLV
                     SKFPKNELLG"
     gene            10846..11067
                     /locus_tag="PWZ48_002105"
     CDS             10846..11067
                     /locus_tag="PWZ48_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261754.1"
                     /translation="MVFDREKMLAHANEVLMSSLKGTELAKIMNMNVNQFYDYRNGSK
                     KIEKARLETLIKFEKAYVYMLDKQKRTID"
     gene            11087..13243
                     /locus_tag="PWZ48_002106"
     CDS             11087..13243
                     /locus_tag="PWZ48_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261755.1"
                     /translation="MAKFKDITESINYVAETVPVVALSLKPQLLEVYKKEEKEAKAKK
                     AKAKVWKEFDALLTKDLPKKNYMVIEDVQKLNSEGVIDEDDAILDSEKYVVLLKSSLN
                     YVADLPEKFKEKLLAGVRFMDSSGQLLVIPDDPNVFESEVEEATDFYSAVEQLEVATN
                     GLERIAETFNSSEEVEFEAVAENVPDEKEALFVDDNKPTEAENQEVSIEETQIQTETY
                     QQPKIDEKPESQSDKENYVYEAEKITSESDVPKNLDADFLEKNFEQIIADKFQPVVLD
                     EINDPVDLNEEMLSIPELKPLLFLAKESMNARISIANNKINSELNKTKEQLILKGKQM
                     LADELLRIEEATSLNGQNQFTNTLSVAKESYEAEASKIDDLVMEKSNIIRKDYDMAKQ
                     AYLDAELKKLEQRYDAEHLPEVKQQEDEYRKATEESLDQQYQEAIESIKNDALKQSQE
                     AKEVVVERIINSLKPEIEKSLTDVQEKTLEIVEKTKSENEAEFHDTKEKTTDVVVKYF
                     ANKEEVEKQVANNTKELETKLVEQQKTIEEYKDRMKEVKDLAEGYKLNSEKLAEQSEA
                     LREDLSRSRSDLAQMAEKTKSLAVATENQSSSKRDFTSKKMATIISVAAMACGTILGS
                     SYFISHNNQQAQAQTQTSISRVENSIGTNTNSASTNSSYEATPTTEFKVGKDVVISID
                     GKTTKAEVISIDGEKAVVKTPDGNQYKIQAHLSDNN"
     gene            13255..13488
                     /locus_tag="PWZ48_002107"
     CDS             13255..13488
                     /locus_tag="PWZ48_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2261756.1"
                     /translation="MREEKIVYPVIQNNQENMTSSQHLQEAQLVYQVNELPQTNETMS
                     KSISLIGLLIVAIVITVGVYRKLNVKGSISDGR"
     gene            13478..14344
                     /locus_tag="PWZ48_002108"
     CDS             13478..14344
                     /locus_tag="PWZ48_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261757.1"
                     /translation="MADNSNYGFKHNLHQKPSETIVRVEWLEKHVNSTYSVTAKRLIV
                     SAIVVAVVVLGSIVYLVSDTIKLKFIFVPTLLMIIGFLILISQYMFIDPSSGSLISGS
                     IRLFKSVVNSARVSMGKKKPFRGTEILKVKQDGTIVYRDGSVALMFNLDGATSLTAFP
                     EEVMAQESKSIQYQNTRDRSLAEIKITSSQPQNTERQTRNMKIQKRNARSNKAVFDII
                     DQQERFVSEYIDGVKTTVIQYLILKSQSKMVLDEGVERLENYTSRGLYYSVTQLSAKQ
                     TKEILSDIYALK"
     gene            14359..16329
                     /locus_tag="PWZ48_002109"
     CDS             14359..16329
                     /locus_tag="PWZ48_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261758.1"
                     /translation="MEIMGRSQLLNFKKKRKTKKEQKTRNSKVVNNTFVSDLTSLVGY
                     QFTPSYIRTGKRWGTIVKLVNTYGMNRNYGFGWMVKAIPEVRVEGVKAYLIEADKPVS
                     QKDQQEIMHENVGGSTNSYENNLEGKNSDAEISKTVMRIMDLNTASLLDSMANKIIDW
                     KLEILLVSDSPNKISEQLIKLQRLYDEKMYGLKLMSYGGDQRDLFDRLLSEPIGNVYE
                     YTSMSSDFAGNDHAIRKGLDDANGWAIGTMSTSYASGVAFMALDDSFTSKVLVASHKN
                     ATIKGYDEKISASSLWGQLIANNAMANHKRTFHIVLNKFDYFGDPTGRTFVAKPSLNS
                     VIEKVNLAEEGLNSLELFGNQKDIGDLYRRNLDKLTQIFYLLAGRTLDSATRVLLKKE
                     LGNFYISQKLWDINYEERSKLLVNSSDTMPVLGDFISYLTNAETKAKKSGTEKEVDRI
                     IVLKDTLRTALDSFSRMFNQRTTLKEIGKSDKLQFYYNLGDLRAETNVMEAQFLNTFD
                     FITSQAKKDDIVMIHGFDKLSIETVGLIKKRLTMLEEDGIRLAFLFDTIGSGEDNEYK
                     EDIETADIFNTDGKIYQSFESDFNYTIIGKMTSGELMKYEKKVAPARKGEDDQKLSVD
                     LRQYLTTGRSNKFQIRRPVDLTNNFVIGEFIL"
     gene            16496..>16668
                     /locus_tag="PWZ48_002110"
     CDS             16496..>16668
                     /locus_tag="PWZ48_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261759.1"
                     /translation="MKPNIKILDRIFLGRDTEVILIQHEEGFEVSIGIQKLQKPHYCN
                     QLYKNFTDEEKARV"
BASE COUNT         6148 a         2353 c         3301 g         4866 t
ORIGIN      
        1 acagattgct gatgcaattt ttcaataact tgttttaatc ttgaatgttt tgaagaagat
       61 gaactagaag acatcaacgc aaataaagcc tttttagata ctttgacggt attattaaaa
      121 ggcggatttt tagggtcaag atgtcctata gcgtaaagat aaattttttg tggcatagca
      181 tccatttcaa caatgctatc tacaagttga ttcgataaag taacagttaa atcaaacttt
      241 tttagattgc tttcggtagt ttcaaatttc gggtttacat tcttttctgt tattttcata
      301 cgattgcctc ttctttctaa gcttggtacg agcttaaaaa cttggaactg gtacgattcc
      361 attgaataag tatagacctg ttttcgatac gttacaataa gaaatgtatc gtaaatttat
      421 aacataactc aatgtcagta aaacacattt tattttttcc aatcaaattt cattgaaaat
      481 acagcagaaa tcgctatttt agaacatttt caaaataaaa atccttttca ataaaatacg
      541 aaattataaa aattaccaaa attaaaaaac aaatattttt ctagttttta gaaataaatt
      601 cacatacttt ttcttttttt ggatatattc ggaataccac atcgcttttt gtccacgttt
      661 gcatcgcttt ttgtccacat tcacatcgct ttttgtccat gttcgcatcg ctttatgtcc
      721 accctaaaat ctatttaaga ttgatataaa gcctttttgg aggttgctaa agttttaaag
      781 attaaaatat atattataaa tataaataaa aggggaagct gaagcttcct ccttttcctt
      841 tttttcaatt attcaaagtt acacaagcaa aataaactcc tagatagtgt tagttttttt
      901 taagattttt tatttttaag attttgaaat aagagaggag taatcctcct ttattaaata
      961 tctgttaata tatctgttaa atatctgtta atatatctgt taggggaggc ttaatctctt
     1021 gggagagtaa gtattaaagg ggtataaagg taacactttt ggacagtaaa ggtaacaaaa
     1081 gtggacagta agggtaacag ttttggacag caaaggtaac aaaagtggac atttgaggta
     1141 tcacactagg ataagacgtt accttcggtt ttcttgattt ggaaggcgaa aaaatagaaa
     1201 attagattag taacaaatct ttgttgactg ttacctttag ttgtgattta atttaggtaa
     1261 caagcagaaa aaattgtttc ctttaattag taccagatta acatgattaa aggagcgcaa
     1321 aaagaagatt ggagagtaat caatgtctca agaagttacc gaaaagatta ttgatagtga
     1381 tgtagattta gtatcagaac aaacaaatgc aattgtgcgt tataacaatg gtctgaatac
     1441 aacagctttg cgaggattta agcctgttga aatggatgtg ttttgggcta ttttatcaaa
     1501 aatgaaacgt caaggaacag cagaagtaac ttttgatttc aacctctttc gttatttggt
     1561 caaatacgac agaagagaaa aggacagttt ttattctgtg ttaaaagatt tgagtgacaa
     1621 actaggtaat ttaacctacc gttttgaaga ttcagacgtc tatgaacagc tgtggctttt
     1681 tcaaaaattc cggatagata aacagaagga aacagttaca attaaggcaa atgaggaatt
     1741 tgaatttatc ttaaactcaa ttggcaaaaa ctttactcgt tttgaacttg ataattttac
     1801 aaaactaaga agtagctaca caaaagaatt gtatcgtcat ctcatgcaat acagaaataa
     1861 agagtggcga aatggttatt gggtagtcaa agttgaagat tttagaagag cattaagtat
     1921 accttcaagt tatagaatga caaatattga taaaagaata ctagagcagg cgaaagaaga
     1981 atttttagct cctgatgaaa acggaataca ggtatttgaa acttttgatt atgaaaaaat
     2041 ctatgcaaag aaaggaagaa gagttgatcg gctcagattc acttttagtg agccagaatc
     2101 aaacttgcca accatttcaa tgcataattg gttagaagaa gattgagaag aagcaggttg
     2161 taattttaat acaacctgct tctttaaaaa agtatgggta acacttttgg acaataaatg
     2221 caagaagttt ttttgtaagc aagtttcagt ctaaataata tttgaaagaa tggctaatct
     2281 atacaaattt taacaactat gggtaacact tttggacaag tcaagaaaaa aaacaatttt
     2341 tcttaatttg ttgatcgact aaaggtaaca cttttggaca acacagaata aaaagtccgg
     2401 tgatatcagt tttggtagga aacatcttaa tatctttgtg agattgaagg taacattttt
     2461 ggacaagtca actatttgtt aaaagttaat atagagtaat tagataagtt ttttcttttc
     2521 gtctgataaa tggataatat tagttatttt attgctaatt agtctttcat gatgactgaa
     2581 atagctaaaa taaatatctc gttggataat tgctgatact ttaagggata tctaaatttg
     2641 ctgattgatt tatttatgtt attatgatat tataataaca taaatgtgtt acgcccgaac
     2701 ctcattttaa aaaaatgaag gggtgagaca atgaatgcta ttaattggca tgaatttgtt
     2761 aactatatgg agagcttact agatgatcta aaaagtttta gtattgctga tgcagtaaag
     2821 catgggttac cagttagtac aacgatgaaa ttacgaggat tgaaaattcc agatgaaatt
     2881 attaagctga gaactaaaac aattattgaa accgcaaaag tcgttgacaa tatgaaccat
     2941 gaactaaaag gcaagaaaac aacttacgag aaagattttg aacgtttttt aaaatctgtt
     3001 attggaaaat atctaggtaa aaaatctttc agtgaattga gagatgaaga gattgtacct
     3061 actccaatgg ttagcaaaat ccgaaaaaac aaagccgagg ggaattacta tcttggtaca
     3121 atagcaaaaa tcattgatta tttggaaaat aaagagcaaa agaaaggaac gaagaaagga
     3181 agataatttt tgtgttaaat attaatgggg aagaatatac cgaaatgatt caaaatcccg
     3241 aacctagaat taccgaaaaa acgtctgatt gtttgctctt ttatgaaagg gggcagacaa
     3301 actaatggaa aaaaacgtca attcaacatc taccaaagat caatacagta ttattggagg
     3361 gttgcttggt gataaggata agagaacacg tcacggatta cgcattatgc ttgcaacttt
     3421 tctaggagtg ctttttttat taacaggctt gggtagctac atttatcaat taggaaacta
     3481 taattattat ctcgtgaaca atacaacacc tataggctct tcattggagt ttgctcgatc
     3541 aggagcagag atcactattt cagatgtgtg gacagatcaa aacagagatg taacagtagt
     3601 aaaactcggt tactcgcaaa acgctagaaa gttgttatct acgcaaggaa aaaactacaa
     3661 cttatatatg gcaacgaaag aaaaagatcg tcctaaaaag cttgaaatga gctatgggat
     3721 gttaagtact gatggggatg ctttcttatt cctaaaaggt aaattagatg aaagagctta
     3781 tcaagttttt attgcaaacc aagtatcact aatttctggt gatgatacag ctgacgatga
     3841 taccaaagtc aatgtgaatg aagcagattc agttacaaaa gcactttctc aatattctat
     3901 gaatgatgtt aataatgaag gagtaatttc ttttgggaaa aaagcaaaac ctgttaatac
     3961 tcctgataat atcaatttca gaataaatcc atattcagat tcaactaatg tctatcaagg
     4021 aagctttctt accaaagatg gcgatattga ttattcaaaa gttgtagcag ttacaagtaa
     4081 gaataatgtt gtaagtaagt tagaagatca gttaaatgct tcaaatacta acatcaagaa
     4141 aattgaagct acaaaagctg agtatctgga acgtttacaa caaaatcctg acgataaaga
     4201 ttcgcaagaa agtattcgtc aactagacag tcgaatggag accgaaatta aagcaatcaa
     4261 tgatacacaa aaattgattg atcaatacaa aaatgctgac tttaacaaag attcttttgg
     4321 agacatgaag acgacattta agtacctaac tctgactgac tagaagtaaa ttattcttat
     4381 tttagaggaa gtgaacacaa acagatggaa aatagtaccg agttttttaa gaatattcaa
     4441 actgaattta atcatgctct cacaccaatg aaaccaccga aacataagtt agtgggacgt
     4501 gatgacgaac tccatgagtt gtcactgatc ttgaatagac cggaaacacc gcaagctatc
     4561 ttgttaggtg aagccggaac aggtaaaaca gcacttgcaa gtgaatgggc tgaaagacag
     4621 cgacaagatg gaaagaaagt acaagtatac aaattatcaa tcggacaatt gcgtgttggg
     4681 ggagatgatg tcctccaaac acgtttagaa acattaattc caagattgaa agaatatgag
     4741 cgaattttaa aatctgagga caaagaaaat gaatttgtcc tcttcattga cgagtttcat
     4801 aaagttgtta gctcttttgg tagtggcaat aaaattgggg gtgattcgct aaaagaaccg
     4861 ttagcaaatg aatcacttca attgattacc gctactacaa gaaaagaata tgaaaactat
     4921 atcaaaagag atgatgcctt agggagacgg ttacaaccgt tagaattgaa tgaactaaaa
     4981 agcgatgttg tcgtcgatat tttgagacaa tggctaaata catcctctgt taaagatgat
     5041 cctcaggctt cttttaaatt atcgcaaagg attcctgatg aattacttca agaaatcgta
     5101 caagcaaaca agctttatcg tgaggatttg tccgaaccgg ctaaatcaat tgccgtaatg
     5161 gaggatttga ttgcaaaaga tcgagtagac ggaaatgctt ataatcgaga aagtctattg
     5221 caaatatttg gtaagtataa tattcagcta gattctgatt ttgatatgga aaaacttcaa
     5281 gaggaattaa agtccataaa agggcaaccg ttagcagttt atactataac aatgttgttt
     5341 aaacgtatga agcttggatt tgatcgtttt aaaggtcgaa agcaaccaaa agcaactgct
     5401 ctgtttacag gatcaacagg ggtcggtaaa actgcaatgg ctaaagccgt ggcaagagga
     5461 ctttatggcg atgaaaataa agttctctta gttaatatgc cggactacaa aacaaaagat
     5521 gatgcaatgc gttttagacg actcataggt actcatgtaa ggcatgatcc ttctgcgatt
     5581 attgtgtgtg atgaatatga gaaagctggt gtagaaacac tcgatgcctt tttatcaatt
     5641 ttagatgaag gtaggataag ttttactgaa agaggatggg atgatcgaga agttcacgac
     5701 actgtttcat tgtcaaatac aattttattt gcgacaacaa atgcaggtgc ggagatgttc
     5761 gattcgttaa acagatatag ctcactatct cgtgttacaa acaaggaaaa ttctaatgaa
     5821 tatagcactg aactaaaaag agaataccga aaagtcgcat cagggatcaa acaagccttg
     5881 attggtgaag gtctaaagcc tgaagttatt aaccgatttg gacgaatagt tccattttgg
     5941 gctttatccg aacaaactct tctggaaatt gcttataatg aaatgttgaa gagtgctgaa
     6001 ttagtacgaa aacatttaaa tatagattta cgtcttccac ctaggaaaga aagagtatat
     6061 ggtcaaaatt tttatgcaga cgatctctcc atgcacttag tttttgacat ggcttcacaa
     6121 gaggattcta aatcaggggg tgcaaggaat atcttgcatt tagttgaatc tgagtttgaa
     6181 gaagcgatta ctgaagcaat aattacttat ccaaacgcaa aaatattcga tgttaagttg
     6241 agtgaagccg gagcgtttga aggaaaaggt gattctaatg ctgaaattat tgcaaagcct
     6301 tccgttgctg aaagtaaaga aaacaagatt gtattttcga ttggaggggc gtagctatga
     6361 aattcaagaa atatttacta caaaacctta gaataaaacg atgggtcgta agaggtctag
     6421 ttattggagg agtattagca gttactttag gcgctccttt tggaatgaat gatccaactc
     6481 ctgacaatca agatagcaaa caagaaacag ttataaataa gaacgttgca tatgcggaaa
     6541 gtaaaccggc agaacaagga atgtggacac tatatgctcg tatcctaatg ggagataaag
     6601 aagttaatga tagtgccgct aaaactggac aaatgaattt gttaggacaa ggtggtatca
     6661 gtgccgattt cccttattct gacatttcta aacaaggtaa ttcgattgca gggggtaatg
     6721 caggtactca actagcatct actttagcaa cttatagtta ctacggatat attgaaacag
     6781 tctcaggaaa tgctattgcc agtcaagcat catccattct aggaagtatt ggtaggacga
     6841 ttggtagttt tatcggatca attagtttaa tcctttctaa tattgctgaa tccataaatg
     6901 aactccttgg aaaatttttc atgagtatga atatattttc tcttatgggg tggggagaag
     6961 gtggatcgga tgtatctaat ccggtatcta aagcattaac tagcttgctt aacaatttgg
     7021 gattaaataa tgaattattt aaagcgatag caagcttatc tatgctcttt ttcacagcaa
     7081 tactaggtta tcaaggaatg aaattattgt cgaaagatgg tgttaactcg aagggattat
     7141 caccagtact tgaaaaatgg attgttcgta tttttgtatt gtttatattc ttaccagtgg
     7201 caggatcgct attgggagtt tatatcaacg gcatccagtc agcaaaaaaa gatggagata
     7261 taccaacaaa tgttgtttct gactacatca ttaacacaag actttgggct tctactcaaa
     7321 atcttgctcc atcatctgat actggtggtg atgtacctga cggaaagcct acatccgaaa
     7381 tcaagttcgt tgatacagat tatgaaccat ctaaaaaatc tgcaatgata agagcgatca
     7441 atactagcag ttatgccaga caaaataatg atctagattc taattcagtc ggtatggctc
     7501 tattaaaaga atggtcatcg aatgatactt tcaatattaa tacttatgcc ggtgatgtta
     7561 atgtcgccga aaatgatgct tatatgggaa gagcgcctgc tgtttggaca ggctataatt
     7621 taggggatgg aaaagaagga agtgcaatta agaaaccaac caatttaaat ggatatattt
     7681 ggtcagcaaa tcaaaatgtg acagacgaca atcgcaagcc tgatggaaaa ggaacagcgc
     7741 ataactttga tgctactcaa ccttggggcg ttgctaacaa tgcaactttt agcacacagt
     7801 cggtagcttt attgctccaa tcggcacttg atacatcggg agctaagttt tatgcgtata
     7861 atatcgctcc ttcaggaatt caaggcagtg ctaagaatat gtcaacagta aaaacagaat
     7921 ggcgtacagc atccttagtt ggtcatggag ctatgggtaa gtttggctcg tggctagcga
     7981 tgaacgctga atcaatcgtt ttagcagttc tttacttagc atgtgcttgg gcgatgttca
     8041 atgtgaatat cgtgaaagca tttaaacgtg aatttattcg gatttgggaa gtagttctaa
     8101 caggatcact tgcatcagga gcaggtgtga tggcaatttc attaggtgtt gttacaacat
     8161 ctctgatcgg actacaatta cctatctttc tgattacttt tattcgtgca tttggtgata
     8221 ctgctgtggg attcattcct aatacattag ttggcgatgg cgtagcaaat attgttcgat
     8281 ctgctatgat tttaggtgta ggatatcttt tagtctttaa aaaaggcgaa aaagtaaata
     8341 atttgattaa tatgcttatt ggcttaccac ttgaagtagc gtttgggttt gatgaacgtg
     8401 ctaagttatt atttggtaat agaaatgatg ctgtgcgagt tgtggacggc gtttctaaag
     8461 caatcaagaa aagccaagga gctaaaaatc cctattatga tggtggcaac agctcagtaa
     8521 aagatatggt tgcaggaggt cttggagcag ttggagcgca aaaaatggct gaacgtttga
     8581 acaatatggg agatagcaat gctgatgcta agtccattgc tgataaagta attcctgaaa
     8641 caccagaagg aatgtcattc aacggtggcg gagttggttc cacaatcaat aatacctctt
     8701 tcaaagattt agttcgtcaa gatcaactac gtaataactc ccaacaatat attgatcatt
     8761 taggacgtat ggttgatgaa aacggaaact ttgtcgatga aaatggtgaa atattaaaag
     8821 atagtaaata cggtaaagat gctaaaggca actttattga cgaaaaaggg cgtttggtcg
     8881 atgaagatgg aaaccttata gatactaatg gtaaaccatt ggaagatagt gaagatggta
     8941 atattggtat ggatagcgaa ggacgtttaa ttgatgaaaa aggtcgctta gtcgatgaag
     9001 atggaaatct tgttgacgaa aatggacgca ggttgctaaa tggtgatttt ggtcatgata
     9061 gtaacggacg tttagttgac gaaaaaggtc gtttagttga tgaagatgga aatcttgttg
     9121 atgagtttgg taataaactt ggtaaacaca gcgatggata taatgtgaat ggagatttag
     9181 tcgatgagga tgggaatatc attgagacga gagatgcgct tgtggcagat cgaaatagag
     9241 aactcgaaca acaacggcat acgccaattg ctgttgcaat tcctgagaat gaagaagacg
     9301 agattacttt agatcaaact catcaagaac tgaggaatga aatgagtggt gaggacgtgt
     9361 cagacagagt gcatcgggaa ttaggagatg gagcatctgg tgaaagtcag tcaagagagc
     9421 ttaacagcaa ctttgataat agtgctattc ggaatgacac agtacttaaa gacagagaat
     9481 cgctaacaaa agaaacggat aaagaacaat cagatgtgaa gaaaaagttc attcctactc
     9541 ctaatgttaa tcaaacagat aaatatgaaa caaatattgg atcaggaatg tcgaaagaca
     9601 atcaatcaat tagagaacct gaactgacaa ataaaaataa tagaacaaat aattctaaat
     9661 ctacttcaga taattcaggg aaatctaatc aatcaaccaa gaaaacatca acagctaaat
     9721 cagccgttga atatctatca aaaactggta aaaatgttta cacctcagca gataaagctt
     9781 tagcaaaagc tccaaaaggt agtctagcag ggataaccta tgaagctaaa aacgaagcta
     9841 ttaaaatagg taaaggaacg gctaaggttg ccaaggctac tgctacagga gcatccaaaa
     9901 ctggtaaagt agtgaagaaa tctaccatgt cagtcaagga ctatattgta aaatacggta
     9961 atcaaaaatc aactgaaaat aagtctgaag acgagtaaaa agaaggagca ttgatttgct
    10021 ccttcttttt actttgattt ctaactgaag atgtcaagga aaatttctat ttatgcaaaa
    10081 aaattatttt aaattgtcta taaatgaatt aaatatttac tgttcaaagt tgtataatta
    10141 aaaagggctt atgctggtaa agaacagaat tattatctaa acaaaaactt gataaaccag
    10201 catttttagc caataaaaaa agtgaaataa tttaatggaa aatctctttt aatcatcgaa
    10261 aatttatgtt attatgataa cataaagatg taaatatcga gacgtctttt tcttagttag
    10321 atcattgaca agtgaacaaa taagatttgt cgatgatata attaacttat taaaaatatt
    10381 aaaaaggagg tttttatatg ttcgattcta ttaaaaattt ggttagcgga atgacgcctg
    10441 atatgaaaac tttagtacaa gttgttttgt atttcttggc attcgttttt tcgttgaaac
    10501 caggattcac tgcaatgaaa ttttttggtg atgcaaaatg gggagatgga ttaaaatatg
    10561 ttggggcgat tattgcaatc tttattattc ctgtaattac aaccgttatg ctttacacaa
    10621 tgggtactaa aacaggtaat gatttgaacc aaaaagctaa tatcatatca gcacttattc
    10681 cagttgttgg tatctatcta gtttcaaaat ttccaaaaaa tgaactgtta ggttaaaagt
    10741 gaattgaaca aatttggata atatgccgat ttgttcaatt tactttttac caaaaaacta
    10801 cgctccaagc gtagtttaga agtgagggaa gaaagtaaat gtaagatggt ttttgataga
    10861 gaaaaaatgt tagctcacgc caacgaagta ttaatgagca gtctgaaagg taccgaactg
    10921 gcaaagataa tgaatatgaa tgttaaccag ttctatgatt atcgaaatgg gagtaaaaag
    10981 attgaaaaag ccagacttga aacgcttatt aaattcgaaa aagcatatgt atatatgcta
    11041 gataaacaga aaagaactat tgactgaaaa aaggagtttt acaataatgg caaaattcaa
    11101 agatattacc gaatctatta attatgtggc agaaacagtt cctgtagttg cactctcttt
    11161 gaagccacaa ttactcgaag tgtacaaaaa agaagaaaaa gaagcgaagg caaaaaaagc
    11221 taaagcaaag gtatggaaag aattcgatgc gttgctgacc aaagatttac caaagaaaaa
    11281 ttatatggta attgaagatg tacaaaagct caacagtgag ggagttatag atgaggatga
    11341 tgctattctc gattctgaga agtatgtcgt tctattgaag tcaagtttga attatgtagc
    11401 tgatctacca gaaaaattta aagagaagct attagcaggt gtccgtttta tggatagtag
    11461 tggtcaatta ttagttattc cggatgatcc taatgtgttt gaatcagaag ttgaagaagc
    11521 tacagacttc tatagcgcag tagagcaatt agaagtagct actaatggat tagaacgaat
    11581 tgctgagacc ttcaactctt ctgaagaagt tgaatttgaa gccgtagcag aaaatgtacc
    11641 agatgaaaaa gaagctcttt ttgtggatga taacaaacca accgaagcag aaaaccaaga
    11701 agtaagcata gaagaaacac aaatacaaac agaaacctac caacaaccaa aaattgatga
    11761 aaaacccgaa tcccaaagtg acaaagaaaa ttatgtttat gaagctgaaa aaatcacatc
    11821 tgaaagtgat gtaccgaaaa acttagatgc tgatttttta gaaaaaaatt ttgaacagat
    11881 cattgctgac aaatttcaac ctgtagtttt agatgagatt aatgaccctg tcgatttaaa
    11941 cgaggagatg ctatccatac ctgagttgaa accattgctg tttttagcta aagagagtat
    12001 gaacgctcgc atctctattg ctaataataa aatcaacagt gaattaaata agacgaagga
    12061 gcaattgatc ctcaaaggaa agcaaatgtt agctgatgag cttttgcgga tagaggaagc
    12121 aacctcatta aatggtcaaa atcagtttac aaatacacta tcggttgcta aagagagcta
    12181 cgaagcagaa gcatctaaaa ttgatgattt agtgatggaa aaatccaata tcattcgtaa
    12241 agattatgat atggcgaaac aggcttattt ggatgctgaa ctaaagaaac tagagcaacg
    12301 gtatgatgct gagcatttac cagaagttaa acaacaagaa gatgaatata gaaaagcaac
    12361 tgaagaaagt cttgatcaac agtaccaaga agcaattgaa tctattaaaa acgatgcatt
    12421 gaaacaaagc caagaagcaa aagaagttgt ggtggaacga attatcaatt cattgaaacc
    12481 agaaattgaa aaatcattaa cagacgtgca agaaaaaact ttagagattg tcgaaaaaac
    12541 aaagtcagaa aatgaagctg agttccatga tacaaaagag aaaacaacag atgtggttgt
    12601 caaatatttt gcgaacaaag aagaagttga aaagcaggta gctaacaaca cgaaagaatt
    12661 agaaacaaaa cttgtagagc aacaaaaaac tatcgaagaa tataaagatc gaatgaaaga
    12721 ggtcaaagat ttagcagagg gttataaatt aaatagcgaa aagttggcag aacaaagtga
    12781 agctttaaga gaagacttat ctcgttcccg aagtgatcta gctcaaatgg cagaaaaaac
    12841 gaaatcatta gcagttgcta cagaaaatca atcaagttca aaacgtgatt ttacaagtaa
    12901 aaagatggct actatcattt ctgtcgctgc aatggcttgc ggaacgatct tgggatcgtc
    12961 ctactttatt agtcataata atcaacaagc acaggctcaa actcaaacat ctatttcgag
    13021 agtggaaaat tcgattggga caaatacgaa ctctgcttca acaaatagta gctatgaagc
    13081 aacaccaact acagagttca aagttgggaa agatgttgtc atcagtattg atgggaaaac
    13141 aactaaggct gaagtgattt ctattgatgg agaaaaagca gttgtgaaaa ctccagacgg
    13201 aaatcagtac aaaattcaag ctcacttatc tgataataac taaggagggc tattatgcga
    13261 gaagaaaaaa tagtttatcc agtaattcaa aacaatcaag aaaacatgac ctcttctcag
    13321 cacttacagg aagcacagct cgtgtatcag gtaaatgaac taccacaaac gaacgagaca
    13381 atgagcaagt caatatcttt aattggcttg cttattgttg caatagtgat aactgtaggt
    13441 gtttaccgta agttgaacgt gaaaggaagt atttctgatg gcagataact caaactatgg
    13501 atttaaacat aacttacatc aaaaaccttc tgaaacaatt gtcagagttg aatggcttga
    13561 aaagcatgtc aatagcacct attctgttac agcaaaaaga ttaattgtat cagctatagt
    13621 tgttgcagtc gttgttcttg gatcaattgt ttacttagtt tctgatacga ttaaactgaa
    13681 atttattttt gttccaacgt tattaatgat aattggattt cttattctaa ttagccagta
    13741 tatgtttatt gatccatcta gtggttcatt gatttctggt tctattagac tgttcaaatc
    13801 agtagttaat tcagcaagag tatctatggg gaaaaagaaa ccatttcgag gcacagagat
    13861 tttaaaagtg aaacaagatg gaacaatcgt atatcgagat gggagtgtag cattaatgtt
    13921 caatctagat ggggctacat cactaaccgc ttttccagaa gaagttatgg ctcaggaatc
    13981 gaaatcaata caatatcaaa ataccagaga tcgttcctta gctgaaatca aaatcacttc
    14041 atctcaacct cagaatacgg aaagacaaac tcgaaatatg aaaatacaaa aacgaaatgc
    14101 aaggagtaac aaagcagtct ttgacataat agatcagcaa gaacgatttg tatcagaata
    14161 tattgatggt gtgaaaacaa cagtaatcca atatcttatc ttgaaatctc aatctaagat
    14221 ggtattggat gaaggtgtgg aacgtttaga aaactatacg agtagaggac tttattatag
    14281 tgtcactcag ttgtcagcta aacaaacaaa agaaatatta tctgatattt atgcactgaa
    14341 atagaggtga gagattaaat ggaaattatg ggtagatcac aactattaaa tttcaaaaaa
    14401 aagagaaaaa ctaaaaaaga acaaaaaact agaaattcaa aggtagtgaa taataccttt
    14461 gttagtgatt taacatcttt agttggttat caatttacac catcttacat taggacaggt
    14521 aagcggtggg gaacaattgt gaaattagtg aatacttatg gaatgaatag aaattatgga
    14581 tttggttgga tggttaaagc tattcctgaa gtgagagtag aaggggtaaa agcctatcta
    14641 attgaagcag ataagccagt gtcacaaaaa gatcaacaag aaataatgca cgagaatgtt
    14701 gggggatcaa cgaatagtta tgaaaataat ttagaaggaa aaaattcaga tgctgaaatt
    14761 agcaaaactg ttatgcggat tatggatttg aatacagcat ctttactaga cagcatggca
    14821 aataaaatta ttgattggaa gctagaaatc ctattagtat ccgattctcc taataaaatc
    14881 agcgaacagc taattaaact ccaacggttg tatgatgaaa aaatgtatgg attaaagctt
    14941 atgtcatatg gaggagatca acgtgatctg tttgatagac ttttaagtga accaatcggg
    15001 aatgtctatg agtatacgtc tatgagttcg gattttgcag gaaatgatca tgcaattcgc
    15061 aaaggtttag atgatgcgaa tggatgggct atcggcacta tgtctactag ctacgcaagt
    15121 ggtgtagctt ttatggcact ggatgattct tttacaagta aagttttagt ggcatcacac
    15181 aaaaacgcta caattaaagg ctatgatgaa aaaatctcag catcatcact ttgggggcaa
    15241 ttgattgcta ataatgcaat ggcaaatcat aaacgaactt ttcatattgt cttaaataaa
    15301 tttgattact ttggtgatcc aactggtaga acatttgttg ccaaaccttc gttaaacagt
    15361 gtaatagaaa aggttaattt ggctgaagaa ggtcttaact ctttggagct ttttggtaat
    15421 caaaaggaca ttggtgattt atatagacgg aatctcgata aactaactca gattttttat
    15481 ttgttagcag gcagaacatt agattctgca actagagttt tattgaaaaa agaattaggt
    15541 aacttttata taagtcagaa actttgggat attaattatg aagaacgttc aaaacttttg
    15601 gtgaacagta gtgatacaat gccagttctt ggggatttta ttagctattt gactaatgct
    15661 gaaacaaaag ccaaaaaatc tggtactgaa aaagaagtcg atcgtattat tgtattaaaa
    15721 gacacattga gaacagcatt agacagcttc tctaggatgt ttaatcaaag aacgacactg
    15781 aaagaaatcg gtaaaagtga caaattacag ttttactata acttaggtga tctaagagca
    15841 gaaaccaatg ttatggaagc tcaattttta aatacctttg atttcatcac atcacaagca
    15901 aaaaaagatg atattgttat gattcacggc ttcgataagt tatctattga aaccgttggg
    15961 ctaattaaaa aaagattaac aatgcttgaa gaagatggaa ttcgtcttgc ctttctattt
    16021 gatacaatcg gttcaggaga agacaatgaa tacaaagaag atattgaaac agctgatata
    16081 ttcaatacag atggaaaaat ttaccaaagt tttgaaagtg attttaatta cactatcatt
    16141 ggaaagatga ccagtggaga gttaatgaag tatgaaaaaa aagttgctcc tgctagaaaa
    16201 ggagaagatg atcaaaaact ttctgttgat ttgagacagt atttgacaac aggaagaagt
    16261 aataaatttc agatcaggag accagtggat ctgactaata attttgttat tggagagttt
    16321 attctttaag ttttatcttt gattttgagt gtataaggaa aggataagaa aaaacatgta
    16381 tatttgagac taaaaacagt cttagagaac atggtacgtt ttgtgaaaag agaactatag
    16441 aaatttcata aagtgaaacc ttactgagtg aaataattat agtggaggtt aaataatgaa
    16501 accaaacata aaaattttag atcgaatatt tctaggtaga gacacagaag taatcctaat
    16561 tcaacatgaa gaaggatttg aagttagtat aggtattcag aaacttcaaa aacctcatta
    16621 ttgtaatcag ttgtataaaa actttactga tgaagaaaaa gcgagagt
//
LOCUS       DANKEF010000054        16257 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000054 DANKEF010000000
VERSION     DANKEF010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16257
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.054"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(160..444)
                     /locus_tag="PWZ48_002111"
     CDS             complement(160..444)
                     /locus_tag="PWZ48_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261760.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(462..1352)
                     /locus_tag="PWZ48_002112"
     CDS             complement(462..1352)
                     /locus_tag="PWZ48_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDL2261761.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFALDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(1372..1590)
                     /locus_tag="PWZ48_002113"
     CDS             complement(1372..1590)
                     /locus_tag="PWZ48_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HDL2261762.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(1560..3260)
                     /locus_tag="PWZ48_002114"
     CDS             complement(1560..3260)
                     /locus_tag="PWZ48_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HDL2261763.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            3527..4300
                     /locus_tag="PWZ48_002115"
     CDS             3527..4300
                     /locus_tag="PWZ48_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261764.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="PWZ48_002116"
     CDS             complement(4381..4950)
                     /gene="thiT"
                     /locus_tag="PWZ48_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HDL2261765.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(5016..5118)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(5124..6095)
                     /locus_tag="PWZ48_002117"
     CDS             complement(5124..6095)
                     /locus_tag="PWZ48_002117"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDL2261766.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="PWZ48_002118"
     CDS             complement(6189..7562)
                     /gene="glmU"
                     /locus_tag="PWZ48_002118"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDL2261767.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(8065..8883)
                     /gene="purR"
                     /locus_tag="PWZ48_002119"
     CDS             complement(8065..8883)
                     /gene="purR"
                     /locus_tag="PWZ48_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HDL2261768.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            complement(9050..9874)
                     /locus_tag="PWZ48_002120"
     CDS             complement(9050..9874)
                     /locus_tag="PWZ48_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HDL2261769.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(9847..10527)
                     /locus_tag="PWZ48_002121"
     CDS             complement(9847..10527)
                     /locus_tag="PWZ48_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HDL2261770.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(10550..11488)
                     /locus_tag="PWZ48_002122"
     CDS             complement(10550..11488)
                     /locus_tag="PWZ48_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HDL2261771.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(11733..12596)
                     /locus_tag="PWZ48_002123"
     CDS             complement(11733..12596)
                     /locus_tag="PWZ48_002123"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDL2261772.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="PWZ48_002124"
     CDS             complement(12738..15311)
                     /gene="mprF"
                     /locus_tag="PWZ48_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HDL2261773.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLP
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            15452..>16257
                     /gene="proC"
                     /locus_tag="PWZ48_002125"
     CDS             15452..>16257
                     /gene="proC"
                     /locus_tag="PWZ48_002125"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDL2261774.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLTDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
BASE COUNT         4759 a         3482 c         2786 g         5230 t
ORIGIN      
        1 aataccgaga aaatggtttt tgaacatcac ttattcaaaa taaaaaaaga ttgtgacacg
       61 aattttgtca caacctttaa atgtattcct agagcaaaga agctaagcga agaaatccaa
      121 ttctgattat ttattgtatt tctttgttca gcatccttgc taaaagccca tattggacaa
      181 agtattcact acacccatga tctcatcttt tcgagaacgt gctttttctg tcggatcttc
      241 tacctcttct gtgtcaattc gaacaataaa tttccgattc atcgtcacgt aagcaccgtc
      301 gttgatcaaa ataaacagtt cgttctcatt atagtctgca caggcatctt tccatacgtc
      361 aaagccgttt ttttcagaag ggatatcccg tttaaccaca gtttttccac ttaattcgtg
      421 gaacgttacg tcataatatt tcattatctc tacctctttc gtcattcaat attttgtact
      481 tgttctctga ttttttctaa gatcgtctta gactggacga cttgattttt aatttcgatt
      541 gcatttgatt tggaaccgat cgtgttgatt tcccgattca tttcttggat caggaaatct
      601 aattcccgac ctacaggaca ttgtgcttcg ataagttcat ctagtttttg tacatggatc
      661 gccagacgat ccagttcctc gtgaatatct ccgcgttcca acaagatggc tagctccgtc
      721 aacaaccgtt gctcatcgac tttcgctcca agaaattcct ccagcttttt ttgatacctt
      781 atttgaaatt ccttttcata gatttctaca aaactatcga gtttttttag tacttctttg
      841 aattcttcag tattcttttt caacactaaa gctaaagctt gaccttcttt ttctcgactc
      901 gcctcaatag aatcaagtgc gcttttcaat gtatctagag ctaactgtgc caattcttcc
      961 atgctctctt ccggtttttc ttctaccgtt acgaagtttt cgtccagcgc gaagcgttcc
     1021 gccatccgac taattgacac ttgattttga ctaccgtatc gttgttgaat tccgtctgtc
     1081 aattgagtga taagtgaatc aatcaattcc cagttgatga cgatctgttt ttcgtttttg
     1141 ccaatataag ctagattcac aaatacttcg attcgaccgc ggcttaactg ctgtttaata
     1201 agctgacgaa aatcgttttc aacaaagttc aggattttag gacttcgtat ttgaacatct
     1261 agaaaacgct ggttgacact ttttatttca atatctaact gatacgcttt tgtttcgcgg
     1321 atggctttgc caaaaccagt catacttttc attcgacagg gtccttcctc attacatatt
     1381 ttctattgct ttttgcagtt gttcaaactc ttcgttcgtc aatgtcaggc ctttccccat
     1441 tttttcatgg tctggcgccc aatctcgaag atcgaatttt ggcgggcgac cattccagct
     1501 gactaggttc agttctttac gccacccctt gttgttttct gacaatatag cgatttcttc
     1561 tacgatttca tatgaaaatt cttgtgccat ggttgttcct ccttgagata atataaatat
     1621 aggatcagta atgtgtcaac tggttgctta agcaattcat gctgcaaacg atccttgcgt
     1681 tttccttgta gcagataatc aaaataaaca gttttcgtgc gctcattttg tattttttgc
     1741 tggtattttc gataccttcg ccattcttca gtacttaatt gaccaatttg tgccaataaa
     1801 tcatccggca tactttttat atcttgttgg cagatcattt gaatcatcat gtctacttga
     1861 tagatttcat tgggcagtaa aaaatgtttt tttgctaaaa gttctagcat cgccaatccg
     1921 catgtctctg gtacgaccct gttcttatat aaattacgca gtttttgcca aaattttatg
     1981 cttttgaaaa aagataattc taactgaatt tcttttgtta agtaagacaa aaatcctcgc
     2041 cagtatgttc ttgcaggttt aacagaatga aatgtatacc agttcccata gtaatagcct
     2101 aatcgttttt tcatttccag atagcctaat tgtaaattta attggttcct ttggctatca
     2161 aaaagcagaa agctgcctag tgtccacaat gtttgacatt tccaatggat aaaaatgtct
     2221 ggcatacgaa tttttttcgc tgggcctggc ccttgcacat ctacgacaaa tgcctctgtt
     2281 gctcctttgt taatcgcaat atcaataggt attttgttgc gatacccacc gtctgcatat
     2341 ttattttttc cgatctttcg ataagccata gcgggataaa atgaagcaga ggcgagtatc
     2401 caatctggta tttcttctgt ttttgtctgt tggatatcag tcactacttc cgctagttct
     2461 ggcagtttag tagacaccgt atataaacgt attttgggat tatgtttgag taaggacaaa
     2521 tccaaattgt ctttgaccat cttttccaat ggagcagaag aaaccccgcc ttcaattagt
     2581 gctgttttcg tcatttggcg tctttctcga tagaagcgtt cacgaaggtc ttcaactgca
     2641 gccatttctg gtaacgcaag cacttgtctt gtcgacagct ttttccataa attcacagct
     2701 tttttcatat ctccttgtaa aatcaatgcg ccgttcaacg cacctaccga ggtacctgtg
     2761 ataatctcaa agttgatatc atgttccttc agcgcctgcc acacaccaat ctgataagca
     2821 ccgtgcgctc ctcctccacc taaaacaagt gctgtatgat agaccagctt tttatttaat
     2881 tgtgtggttc cttgatagcc gtttttatga aaaatacgca accattcttc tgatagagga
     2941 gattgaaata aaaaacaaca gttttcttta ataaaaaaac gagcgcaagc ttccatatac
     3001 cgaaaaaatt ttttccattc aaaagtttca ccttcaatca acagattatt aaaaataaca
     3061 tctcctctac ttttagcaat agtaaaatag acggtatatt ttttcgatac tgctttaaaa
     3121 gcacattgct tttttgcaga acgccaaaaa gcgtctctac tttcttgatg actttgaaaa
     3181 aatggaaggg gttgttttgc ttgctggata ggcgaataca gcgacatttt ctgtgaaaag
     3241 taaacttttt ttatctccat cgcgctctcc tttttcttca agtataccag tacgtcataa
     3301 aaaggtcatg agtctcgtat tcgactttgt agattccata agaaaacaat tcataaaacc
     3361 tcctttaaat aaaaaaatac aaaaatcaca aaaattaatt gaaaatgaga gtgttttcaa
     3421 tgaaaacgtg attttgtaat ggaattttaa aataaaagaa acaagctttt ttaatgcaaa
     3481 tgttgtaaaa taaaagagat taagaggaaa taagaggagg aacatgatga ctgagtcagt
     3541 ttatgtacat attgatacca caagtaatgc tgttttaaca aaagggctga ctgctactga
     3601 ctttgcaaac agtatcgtgc attttcctca aaacttgctc ttgcttgatc catctgcttc
     3661 tgctggagaa tatgaatccc atacagggct gaaagtaatc cgtggtacag aaaatattca
     3721 gcgtttcttt tcctcttctc gtaacaggcg tggattcgat gaattgaagt ggatcgactt
     3781 tactgattta acgatgctga aagaattgac acctttagaa atttcagaat tattatattt
     3841 tggtcatatg aaaacacatc ttcattcgcc gtttttttat aaactacaga ataattttgt
     3901 atacttcgat cttaatgatc agctgaatcg gatttattat cgctatttag atgaatttta
     3961 taggattttt gctagtaaat taagttcgat cttgtcagat aagatcaatg taaaaaaatc
     4021 ttttttcaaa aaagaaacac ctgttgaaaa aatcagtgtg gaattattga aagaaatgcg
     4081 taatttgatg caggaaggga tcattttttc ttttgatcaa gttggtttga tcgatggtaa
     4141 atatcgtatc ccaatctatg tgattgaaga ttatatttgg aaattaaaag gcacccggta
     4201 tcgagaagag gaaatcatcg ctactttaat ctactctgtc accgatcgac actggcaatt
     4261 ggtagaagat gccgatcttt cttatctgcc attgaagtag atctatttct tatcaaaaaa
     4321 taaaaaggaa acaagaaact aggacatgat tctgtctcaa gtttcttgct tcttttttat
     4381 ttacagctcc tgtcgtttat ctggcacaaa aatcgaagga gtcgtttgtt ggatccctaa
     4441 taaaacgatg atcgttacaa tagctgtact caacccgctg gcaccattca ttacgaaaga
     4501 aaagagccaa ggattcatcc cccatagtgc atacgctccc cagaaaacca ctcctgcaat
     4561 aaaatgccaa aaatatctgg aaagtgcgcc aatcaatgca ccaaacacga tgctttttct
     4621 aatttgtttc gtattatcgt ctacatagcc tttttggaca tttgaagcca tgagccctgc
     4681 tgcgcctgca aaagtatagg caatcgggta ctcaatcaac acttgaacga cacttaaata
     4741 ataaacttgt cccaacggaa aatgtaggat gccccaaatg aaacctgcca gcatccctgc
     4801 ctttgtccct cttcttaacg caaaaagagt caatggaatc tgccctaatg aaatagaaaa
     4861 actgctgccg atctggatcg gaatcaacga caacaccatc gctaacgctg caacaatcgt
     4921 tccttctacc caaactctat tttttcccat aaaaaaactt cctcctttga ataaaaaagt
     4981 cacaaaggag gaagcagctt tcgaaaatag tcaaacagct gtttattcac aattcctacg
     5041 ctcgcataac cgatacaggt acgaagggtt tagaaataga ttcaatctca gcccaattgg
     5101 actccccttt gtgagtttat ttattattta ttttttgttt caaacaatgg actaactggt
     5161 ttgttttcat ggatacgttt gattgcatct cccattagtc cgccaacact tacttcatcg
     5221 attttgtcga ttttacgatc ttctggaaga tagatcgagt ctgtaacaac tagacgctcg
     5281 atagctgaat cttctattct ttgtaaagca gggccggata atactggatg tgtacatgaa
     5341 gcatacacac tagttgcacc agcttctttc aaagcattgg cagccaatgt gatcgtacct
     5401 gctgtatcga tcatatcatc gatcagtaca caagttttgc ctttgacttg tccaatgata
     5461 ttcattactt ctgcaacatt tgcttttgga cggcgtttgt ctatgatcgc aatcggagat
     5521 ttcaaaaatt ccgctaattt acgtgcccgt gtgacaccac catggtctgg tgaaacaacg
     5581 acaacatcat cgcctttgat tcctttttct aagaaataat tggcaataag tggcgcaccc
     5641 atcaaatgat cgactggaat atcaaagaaa ccttggattt gaactgcatg aaggtccaaa
     5701 gtcagcattc ttgttgcacc agctttttga atcatgtttg ctactaattt cgctgtgatt
     5761 ggttcccgtg cacgtgcttt acggtcttga cgcgcatagc cataataagg catgactacg
     5821 ttgattgttt ttgcacttgc tcgtttcagt gcgtcaatca tgatcagcaa ctccatcaag
     5881 ttatcattga ctgggctgct cgttgattga ataacataga catgcgatcc acgaatactt
     5941 tcttcaatgt ttacttgaat ttctccatca ctaaattgag taacagatga ttttcctaac
     6001 tcgacgccga ctgctgctgc aattttctct gctaatgggc gattggaatt taacgcaaaa
     6061 atttttaatc ttgggtcaaa gtaatgtttt gacatgggga cctccacttt ctttttcaca
     6121 actctagaat tctacctaat aaatagtagt catatttttt aaataaatca agtgattttg
     6181 caaaagtctt agtttagata agggagtttt tttgcatatc cttctttatt gacttgtctt
     6241 gctctcgcaa ttgccagcgc atattctgga atattgtctg taattgttga cccggcagca
     6301 atcgaactgt ttttagctac ctcgacaggt ccgatgatat tcgtgctaga accaataaag
     6361 ctgtggtcgc cgacagtcgt atgatgtttg ttctttccat catagttgac gaagacaaca
     6421 ccacatccga cattgatttc ttctcccagc gtagcatcac caacataagt caagtgaccg
     6481 actttagttc ctttgccgat ttgagcattt ttcacttcca caaagtttcc gatatggacg
     6541 ttttcgccaa tttcagcttt tggtcgtaaa tgtgcataag gtcccacatc tgcatgtttc
     6601 ttgacatggc tgctttcaat gactgagttt tcaatgacga cgtgatcctc aatcctactg
     6661 tctacgattt ttgaatgtga cccgatgaca caatcagaac caatgactgt atttccttgg
     6721 atttgtacac ctgcttcgat caatgtgtct ggaccgattt ctacgcctgc atcgatgtat
     6781 gtcgttgctg aatcgacaaa gcttacgcca ttgaccatat gcatctggtt aattcgcttt
     6841 ctcatgatct catttgcttt agctaacgcg atccgatcat tgactcccat tgattcttca
     6901 aaatcatccg tttgataagc agcaactgta tggccttctt cttttaagat ttcgatgata
     6961 tctgttagat agtattcacc ttgagcattg tttgttccaa ctttgcttaa cgcatcgaac
     7021 aaagcttcgt tgtcaaaaca gtaagttcct gtattgattt cttgtacaag agcttcttct
     7081 ggtgttgcat ctttttgttc cacgattttt tctacgatcc caatatggtc acggatgatt
     7141 cgtccatatc ctgttgggtt ttctgcttgt gccgtcaaga tcgttgcact tgcatttttt
     7201 ccttggtgat attcaaaaag attgttcagc gtttcagtag tcaataatgg tgtatcgcca
     7261 ctgataacta acgttgttcc tttttttcct tccaagaaag aagcagcttg caaaacagca
     7321 tgtcctgttc ccaattgttc tgcttgtaac gcatatttac tgcgttctcc tagttgtgct
     7381 ttaacttgtt ctgctccgtg tccaacgatc gtaatcactt catctggttt cgtttcactg
     7441 acccgattaa tgatatgttc taccataggt ttaccagaaa cgggatgtaa aactttatat
     7501 aattttgact tcatgcgggt acctttccct gccgccaaaa taattgcata acgtgcgtcc
     7561 aactgcgtca ctcctaaata tagtttgctg atttcagttt atcccagtat acccattttt
     7621 tcaacaaaac aagtgatttt cttttatccc agcgttttct ggataaaaaa atagatagac
     7681 cataacgggc ctatctattt ggtaaccggt gctctcctgc caactcctcg gtcacaagtc
     7741 acactactca taaaacatga aaagctgttt tttgaaatcg tttcttgtga cttagagtta
     7801 aacggtaggc ttacaacctc ttcattaaac gtttttcaat cagctgacct tcggtattag
     7861 ctcaataaac ggcaaaatat gaggagcaat ttttccgttt attgagctaa tactcaggac
     7921 aggacgctga tttcacaacc tctagataaa cggtgttcaa aagccagttt ctcggtatta
     7981 agccaaattt atgaaaaaat gtggaaagcc attttcacaa aatttttctt actactcgaa
     8041 actaagcagc tttttcacaa cctcttattc tgaaaaataa tttcctggca caaccgtaat
     8101 atttttcgtt tgagtatcta catctttgac ataaagcagt gacgtataat catcgatggc
     8161 gcgatgaccg ttgaattttg attcggcaaa tacagtgatg ccaacaagtt ctgattcgaa
     8221 ttcttcaatc aagctcttca ttccgttgac tgttccgccg cctttcatga aatcatccac
     8281 tacaaggaca cgtgaaccac gtttcaaact tctcttagac agttccatct tttcaattct
     8341 ttcagaagat ccagacacat agttgacact tactgttgat ccttctgtta tttttgagtc
     8401 ccggcgtaca ataacgaatg gcgcattcag ataataggac acagcttggg caataggtac
     8461 gccttttgtg gctacagtca ttacagcatc gattttttca ccaaggtatt tagaagcaat
     8521 gattcttcca acttgacgca gcagttcagg ttctcctaac aaatcagaga gataaacata
     8581 tcctcctgga agcagacggt cttgttctga tagacgttct gctagagatt caatatattt
     8641 tttcgcctct tcatatgaaa tttcagggat gaacaataca ccacctgcag cacccgggat
     8701 tgtttccaag gttccatacc ctcgttcttt aaatgttttc ttgatgattc ccaaatcttc
     8761 actgatagaa gatttagcag attcataacg ttcggcaaag cttgttaagg aaatcaattc
     8821 atgcgggtga ttcaataaat actgtgtcat gtcaactaga cgttcactgc gacgtacttt
     8881 cacctattcc aactcctttt aagaaatttt atcgttttac attatataag tttttcttca
     8941 aaaattcgag cattttatga aaaaataaga aaatagttcg gtatttatta aaataaaaag
     9001 aagcctataa aaaatttttc gtttttataa ggcttctttt ctttataatt tagttatttt
     9061 ttcttctgaa ggcaaccaat attcgtttga tgatatttac aatcaggaac aaagcaataa
     9121 agactaatgt gattgttgct cctggaggtg tatctagata aaacgagcta gttagtcctg
     9181 acagcatccc tagaaatcct acacaaacac tgatagcaat cacgacattg aagcttttgc
     9241 ctaatcgcat accgattgct gcgggaagaa ccatgattgc ggatatcaaa agtgcaccgg
     9301 caatcgggat catcacagca atagccacac cagtaatcac attgaacatc atcgacatca
     9361 aatgaacggg tagcccatcc acgtgggcgg tatcttcatc aaaagtcaga acatacatcg
     9421 gccgtttgaa taaaaagaac aatataccga tggcaacgaa taagattcct aaaaagatga
     9481 cttgctccca tgtaatggtc acgatcgaac caaacagata ggattggata cttgttgctg
     9541 aattccctcc gctcaaattc atcaatacta acgctagcgc caaaccacca gccattaaaa
     9601 tggcaatgga gatttctgaa taggttcggt agatcgttcg cagatactcc aatatcacag
     9661 ctgctaatac aacgactagc atggtcgtaa gtgtaggatt aaggttcaaa aagaatccta
     9721 acgctacccc tgctaaagag acatgagaaa gcgtatcagc catcagcgat tgtcttctga
     9781 tcactaaaaa cacacctagc atcggggcga ttccggcaat gaagatagcg gcaagaaacg
     9841 ctcttctcat gaattcatat gaaagcaacg ccattgagaa tcctcctttc tcaccagtcg
     9901 gatctgtctg tccgcatact gtttgatatc ttcatgatca tgggtgatca ttagaactga
     9961 tttcccatgg acatgcgcac tatgtcggag aagacgataa aattcatttc tggattcttc
    10021 atccatacct gttgttggtt catcaagaac aaataagtca gggtcagtag caaaaatacg
    10081 cgctaaactg attcgctgct tttgtccgcc agacaattca cctattcgcc gatttcgcat
    10141 atcccacatc cctacagctt ctaaagcacg ctgtacatgt tgatgatctt tttcagagag
    10201 acgcttgaac cagcggcctc ttggatatct tcctgaacgg actaattcga tcaccgtact
    10261 aggaaaacct gcattgaaag atgcaacttg ttgaggaata taaccgatac ttaatttttt
    10321 tccttcttga tttttctcag caatctttat cattcctttt gaaggtttca gtaatcctaa
    10381 cgatgctttc accaaggttg acttggctgc tccattttct ccagttaaaa tgacaaattc
    10441 acccgcatct acatgataag agacatcttc tagtacaggt tcatcatcat agtaaaaggt
    10501 caaatcagat atttcgatat aacgcacctt acaacctcca ttctaaaact taattgatac
    10561 tttcttttaa agctttcaga tttttttcca tgacagaaat ataattttcg cctgctttga
    10621 tttgttcatt agtcaagctt tctaaaggat tcaacacttc tagcttcact cctgtttcat
    10681 tcgacaatgt ctcagctacc ttcgaagagg cattttcttc aaaataaatc actttgatgt
    10741 tgtttttctt cacaaattct tttagctcag ctaagcgact tggtgtgggt tcctgatctg
    10801 gtgaaacgcc cgaaatcgct acttgtttta agtcgtattg attcgctaaa taagcaaaag
    10861 cagcatgctg agtgacaaat gtgcggtttg ttgcatcttt tagctcatcg gagtattttt
    10921 gatccagtgc ttcaagcttt ttgatataag catcggcatt cttcgtaaaa gtttcctttt
    10981 tatctggata tttttcgcct aattcttcag caatcgttct gacttctttt atagctaata
    11041 ccggatcgag ccaaacatgt ggatcgtatt catgtgaatg accatgccct tcttctgatt
    11101 cttcatgttc gtctgcttct tgcgattcta aacggtcgat tcctttagct gcttcgatca
    11161 tcaaggtctg tttgctatcc actgaatcct ttaacgaatc cacaaaaaat tccatgtctg
    11221 aactattata cacgaaaaca tctgcatcgg aaatttctgc tctttctttt gcacttggtt
    11281 cgaaatcatg cggctccgtt ccagcaggga tcaaaagttt cacgtttcct tcatccccta
    11341 cgatctcttt tgtaaaatca tacatcggat aaaaagtcgt aacgatcgtc aaatcctctt
    11401 ttttgtctgc ttccttgttc gtatttccac atgctgcaaa cagtaaagca cctatcatta
    11461 ccgtagctgt caaaagatat tttttcattt atatgctcct ttcaatgaac aaatcggaat
    11521 tttacgattt attaacgtga actaatgtat caaaataaaa aaacacagtc aatagaaatc
    11581 catcaatttc tgatttaagc atgtatattc ttgattaaat cgtaaaatgg atgagttgct
    11641 gctcatatct agccatttca ctacaataaa aaactgaaga ataccatttt aggattcctc
    11701 agtttttcat cacctatttt acttcttata ttttattgca gggttcgtac taaataaact
    11761 tcctcacaaa aaccttttaa cccattatat actctctgcg ctcttgaata atgttgacac
    11821 aaggcaaaaa cggttggtcc gcttccactc atcaatgcag catctgctcc gtacttgatc
    11881 atacgctctt tgatttgatg gatgactgga tgccgctttc ccgttatatc ttctaaactg
    11941 tttcccattt ttttgatcat ctgctgataa tcttgctctc ggatggcttc agcaagtccg
    12001 tggatgtctt gatgttggat ggcttgcgta tctacttctc taaacactgt acgtgtagag
    12061 acactcattc ttggtttgac caacacaacc cagcactgtg gcatagaagg taatacttgg
    12121 atttcttccc ctttgccacc gacaaatgcc gtacttccgt agacacagta aggaacatct
    12181 gtaccgactt ccaaaccgat ttctgccagt tcttctaacg aacagcccag attccataat
    12241 tggttgatcc ctcttagagc agctgcacag tctgtacttc ctccgcctaa tccagctgct
    12301 actggaatat tcttcttaac aaagatcttg acaccggttt tgatattgta tttatttttt
    12361 agcaataaag cagcttgata aacattgttc cgttcatcta ccggtaaaaa tgctttattt
    12421 gtttctacaa tgattttgtc ttctgttatt tcttcaaaaa atagatgatc tgccaagtct
    12481 acgctcgaca tcaccatttc caactcatga tacccatctt gccgtctata taatacatcc
    12541 agtcctaaat ttatcttggc tggcgctttt tcaatcaatt ccatcctctc acccattcga
    12601 aaagtctaag tcgcaagtat tctatcatag ggaaaaataa aagtatagct tatgtctgcc
    12661 tagtggttga ttttgtcatc gtttcaacag ctttcacaga attttctctt cataatccaa
    12721 acaagttcta gtgattctta taattctgtg agcacttctt ctgccaaact cggaacattt
    12781 cctttttctt ttcctatcag actgagtagc tgcatggtag caaatccgaa attacttcct
    12841 ttcctataag caaaataacg tccttcccaa tgatcgacat attttaattt cttttcataa
    12901 gcagactgga aggaatagaa aaaattacca taattatata cgatatgcat gactttttct
    12961 tgaaagaaag aaaaatctgt ttctccaaca ttagctaatg gagctaagcc tagattggct
    13021 tccgagtagc ccattttctc gtatacaata aacagattgg ctaacagata gtcagctaat
    13081 tcctcagatt ctttaggtaa gacccgcaac aaatcatatc ccgcctgctt cttcgtcatc
    13141 ggctgagcgg tgatgaagcc aacgactttt tgatctttac ggacaatacc gacaccgcta
    13201 gataatacat atgatttatc aaatcgtccc acagagaagt atttttctcg tgcaccttga
    13261 agccattttt gtgaaacttc atttacagct tgatgtaatt cttccggcag ttgatcgtac
    13321 caagtaaaat ggtatccttc tttcatcaaa cgttgatatt caagttgctg actcagccga
    13381 gacaagtctt ctgttgctag atcaacaatc cctgcttcac ctactttcgt gaagtgaaaa
    13441 cccaattcat gaagcatcac tacgtagctt tctgatatct tataaaacgc aagctgatag
    13501 cctaaacaat ctgcttgttt cataaactga ttcgttgctg cccgcatcat tttaggattg
    13561 ccaactggat ctcctaaaac aaagcatttg tttcctttga tctgataacc aaaaacgact
    13621 tgatcttggt cgtcttcttg gtaataaaaa taactgtaac ctgacaagcg aagctgatgg
    13681 cttgtagctg tgcctccgta tgtttccaat aaacggtcaa aacggtcttt ttcgtactca
    13741 ctgccaagct tcgtagtatc ttcagctaaa tattgataaa gcgccactaa agctagcaat
    13801 gagatgccca aaccaataag ccctgagaac cacacatcat ctgaaggaaa caaaataaaa
    13861 tgatttcctg ttattcgacc gactaaaaag cgtccgctgt gatagcctgc aattgcgtaa
    13921 acgataaaca aaaagccgaa caagctagca tcaaagatta tagctcccca agaataaacg
    13981 aattttgttc gataaaattc ttttctggat aaccacacag ctaacagaat caagacatat
    14041 accaatatca actgccaaga taacgtacgt gtgattgtat ttaaaatacc aaaagatagt
    14101 aaagcaatcg taggcagaaa tgcctttttg acttttactg aaagtgctct agcaaggccg
    14161 agtaataaaa agccaaccaa tagattcact gtctgatcta aaaaattgaa tgagaacggc
    14221 aataatactt gaaatagccg actgattgca gacagattag tgattgttga tagaagtacc
    14281 atcatgatcc ccgcaaaata gacagcactt acgagcacga tatgcgcaac cttttggaaa
    14341 aacagtcgcg gaatgttatc taaaaatcga ttgattttga ctcctgtatg tgtgatgaac
    14401 aatagaagtc ccgtcataaa aggtagaaca taataaaaca accgataata taatagccaa
    14461 actactgcta catcttgccg aacaggcaat tgactcattc ctaagatcat caaaacgtca
    14521 aaagtcccca ttccacctgg gaccatcgtc agcattccta tcaatgtagc aatcatgaac
    14581 ataggataga tcgaaagcat cgaaatatct attctcatca acttgccgat gatcaaaaat
    14641 accgcaaggg ctcccgtcca ttgtccaaag gatgccagta gcagaccaag caagcctttt
    14701 ggataaaact cgaagaacaa tgtctttctc tttagataag caaaaaagaa aatgactggt
    14761 gctaaaaaac taccgccgaa aagccagatc caatagcttc gaaaaatact ttctgactga
    14821 ataaaaaaga catcaataaa tgtaagaaac gaccatacag acaaaccagt cagcataaat
    14881 agtgcgactt ttgatacagt ggctaaaacc atttttctat ttgtctgctt tccataaaaa
    14941 ctagcccgca gacttgctcc taccacacca ccaaaaccgg cgagattgtt gatcgtgttt
    15001 gttacccacg cagcaagaag aaactctttt ctttccattt ttggttttcc ttgtttttct
    15061 aagactctga tcgtcaccca atcatacaac aacatcggag tgacacccag cagtccagcc
    15121 agaatcatga gcaggatcgt catgcggctt tgttgtccca tcctttgcca aatatcttgc
    15181 caactcatgc cattagcaat atttgcgact tgatttgcca caaagactaa cacggagccg
    15241 aaaaaaatca gttttaaaat cagactatgt tcctttatcc attgcagcat caagtatcgt
    15301 ttatttttca cattttccca ttcctaactt gtatactttt gtgtcatacc ccaatgttat
    15361 cttatttatt ttaatttttc cactttagac ctgttctttg cttgcgatga tagaaaaaga
    15421 aagatatcct aatgctaaag gagaggataa catgaaagta ggatttatcg gcacagggaa
    15481 tatggcacaa gctattattg atggtttatt aaaaaaagga acagtctcga aagaagaggt
    15541 gttcgtttcc agtggacatt ttgaaaatgc acaaaaattt gctgctgaaa ctggcgtcca
    15601 tgcatgcaaa agtaatcagg aagttgcatc acaatcagat atcgttcttt tagccgtgaa
    15661 accaaaaatc attgaaaaag tattggaaga actaagagaa tatagccaga aagtcttatt
    15721 catttctatt gcttcaggaa aatcattaac agatttagaa acagcgcttg aagatcctca
    15781 ggcctccatc gtacgtgtga tgccgaatgt caatgtcagc gtaggagcag gcgtttctgc
    15841 gatttgcaaa aatgaagctg ccacggagga acagcttcag caagctacga tgatttttga
    15901 agcaatcggc agcgtctatc ctttagcaga atcagatttc agtacattca ttggccttgc
    15961 tggcagctca cccgcattcg tttacatgct gatcgatgcg atgggacgga ctggtgtact
    16021 gaatggtttg tccaaacaga cagccactga gattgctgca aaagcaatta tgggttcttg
    16081 tcaaaaattc ctttcaagtg ataaaaatcc atgggaactt gtagaccaag tttcttctcc
    16141 tggcggtaca actgttgcag gtgtagttgc cttagaagaa gcaggattca tcccagcaat
    16201 catcaaagga atcaatacga cgatcgagaa agatcttgag atgttgaata aaaaata
//
LOCUS       DANKEF010000055        15229 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.055, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000055 DANKEF010000000
VERSION     DANKEF010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.055"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            278..787
                     /locus_tag="PWZ48_002126"
     CDS             278..787
                     /locus_tag="PWZ48_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2261775.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="PWZ48_002127"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="PWZ48_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2261776.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="PWZ48_002128"
     CDS             2322..3302
                     /locus_tag="PWZ48_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2261777.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="PWZ48_002129"
     CDS             3424..4842
                     /locus_tag="PWZ48_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HDL2261778.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="PWZ48_002130"
     CDS             4913..6382
                     /locus_tag="PWZ48_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2261779.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="PWZ48_002131"
     CDS             6392..6859
                     /locus_tag="PWZ48_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2261780.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="PWZ48_002132"
     CDS             6895..7767
                     /locus_tag="PWZ48_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2261781.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="PWZ48_002133"
     CDS             7848..10382
                     /locus_tag="PWZ48_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2261782.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="PWZ48_002134"
     CDS             10538..11311
                     /locus_tag="PWZ48_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2261783.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="PWZ48_002135"
     CDS             11304..12095
                     /locus_tag="PWZ48_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2261784.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="PWZ48_002136"
     CDS             12105..12596
                     /locus_tag="PWZ48_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2261785.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="PWZ48_002137"
     CDS             12614..13024
                     /locus_tag="PWZ48_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDL2261786.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="PWZ48_002138"
     CDS             13028..14428
                     /locus_tag="PWZ48_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HDL2261787.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="PWZ48_002139"
     CDS             14451..14825
                     /locus_tag="PWZ48_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDL2261788.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2134 c         3045 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactccta
//
LOCUS       DANKEF010000056        14798 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000056 DANKEF010000000
VERSION     DANKEF010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14798
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.056"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            143..400
                     /locus_tag="PWZ48_002140"
     CDS             143..400
                     /locus_tag="PWZ48_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261789.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(632..1063)
                     /locus_tag="PWZ48_002141"
     CDS             complement(632..1063)
                     /locus_tag="PWZ48_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HDL2261790.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
     gene            complement(1414..2106)
                     /locus_tag="PWZ48_002142"
     CDS             complement(1414..2106)
                     /locus_tag="PWZ48_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2261791.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(2543..3112)
                     /locus_tag="PWZ48_002143"
     CDS             complement(2543..3112)
                     /locus_tag="PWZ48_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2261792.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(3186..3509)
                     /locus_tag="PWZ48_002144"
     CDS             complement(3186..3509)
                     /locus_tag="PWZ48_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261793.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(3663..4475)
                     /locus_tag="PWZ48_002145"
     CDS             complement(3663..4475)
                     /locus_tag="PWZ48_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261794.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4829..5677
                     /locus_tag="PWZ48_002146"
     CDS             4829..5677
                     /locus_tag="PWZ48_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDL2261795.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5822..6763
                     /locus_tag="PWZ48_002147"
     CDS             5822..6763
                     /locus_tag="PWZ48_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HDL2261796.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6753..8924
                     /locus_tag="PWZ48_002148"
                     /pseudo
     CDS             6753..8924
                     /locus_tag="PWZ48_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(9036..11207)
                     /gene="copB"
                     /locus_tag="PWZ48_002149"
     CDS             complement(9036..11207)
                     /gene="copB"
                     /locus_tag="PWZ48_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HDL2261797.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(11227..13413)
                     /locus_tag="PWZ48_002150"
     CDS             complement(11227..13413)
                     /locus_tag="PWZ48_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HDL2261798.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(13413..13622)
                     /gene="copZ"
                     /locus_tag="PWZ48_002151"
     CDS             complement(13413..13622)
                     /gene="copZ"
                     /locus_tag="PWZ48_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HDL2261799.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(13635..14075)
                     /locus_tag="PWZ48_002152"
     CDS             complement(13635..14075)
                     /locus_tag="PWZ48_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HDL2261800.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(14149..14688)
                     /locus_tag="PWZ48_002153"
     CDS             complement(14149..14688)
                     /locus_tag="PWZ48_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2261801.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         4284 a         2991 c         2564 g         4959 t
ORIGIN      
        1 ttttctgtcc acgttcattt tactccaaag ttttcctcat ttcaaaagaa aatcacagaa
       61 tacacgaaga agcgtttcta tttgtataaa ctatatcaaa gttctatact taaattatca
      121 aataaaaagg acgtgaagat aaatgtcaaa tttagaaaac aaagaagaaa aagttgtaaa
      181 caaaattgtt tctgttgtca ataaactgga taaagagtta gacgaacttg atacgttatc
      241 tgaaaatcca gaaaaaaaac ataacctaaa aaaatggtta gttgaacgaa aggctattca
      301 tgaaattaaa aaagttcttc atgaagctga taaatatgaa aaatatgatg aaaaagaact
      361 tgataaagaa tttaaagaaa tcaatgactt attactataa ttgatttata cgactcattt
      421 ttgagtcgtc agactgcaaa caacactcta aatttaggag tttgtctaca gtctgaatag
      481 attttaaatt tattgtccat aatgttatta tgaatttata gcattaagaa aagagataag
      541 actttacatt tttttaggaa tgtaaagtct tatctctttt cttaattgta caattagtat
      601 tatctgcact actttctaat taaataaatt tttagttatt aacttgatta aaatcagaga
      661 taatatcatt taattcagtt tgaagggttg taacgattaa atggaaattt tcttcatctg
      721 ttttattata tggggatgtt ttcgttatat ataacaactt ttctttttca attttcatta
      781 ataactctgt cgaagtctgt actttatctt gataattaaa tgcagacata ccactttgta
      841 taattgtaat tattgctgaa gcaattgaaa caatcaatag taaagacctg tgttcggaag
      901 cctgatcaat taatattggg atactagctg ataagactat ttttataata tttccgatac
      961 caattactct attataaaat cgaatttctt tttttaatct atcgattgta gaatcaatag
     1021 attcgataaa cgctttttga tccatcattt tctctccaat cattttatta aaatcattaa
     1081 caaaaatatt ataataggat ttaagtaagt tctaactaat cacatataaa aataaaaaaa
     1141 catttgtatt ttatcaaagt tttattatac ttgtcaattt gtgtacatcc taaaatataa
     1201 gctatttgtt tacttctgta gaatatatac tttgttgtta gataagttgc acatgtttat
     1261 agaatctaaa taataaattt ttatgtaccg cccctcaaag tgaactgaac cccaaaagtt
     1321 gaactattta atggactgtt tccgatattc tactggagat agtccattaa atagttcaac
     1381 ttttggggtt cagttcaaag agggggggat tttttatcgt tgtggaatat ttaaatacca
     1441 acgcttgtca tgaaaatctt gtgctccacc cttagtattt ccttctggat cgtttgtcgc
     1501 acgcatcatg acgtatactt tcttattagg gaaatcacgc atattgaaag atacatgata
     1561 gccaacattt cctaaagtat tataagcttg atttacgtct ggtctataaa tcccatcagc
     1621 tcttactcta gctagttctt ttccagtatt gtagtccata atgaaaatat actcgtattt
     1681 ataattagca atgtgccatc cagctacatg caagtttgcg ttttcgattt ctccgaactg
     1741 atcaatgtgg gcgtaatttg ttccatctgt cagcgtagaa tttgcagcac ctgctcgagt
     1801 tggatcaatt actggtttat cctccgaagt agttggatta tcatccgtaa aaccatgagc
     1861 caaatcatag gctagttttt ctttacttac acccatttca gaaagataac cgtaaggatc
     1921 tgtatgatcg ccccaaatat tttgtgttac ccataaatgc gatttgattc ctggttggtt
     1981 ataaggagtg tctaatgtta atggaatacc atatttcatt gctgaatctc ttgccaattc
     2041 aacgtatact ttatagttct tttcaaaagt ttctttatca tgtgtatgtt gtaactcgat
     2101 ttgcacagga cttttggctc acgaggttgt cctactctac atcttgctga tagatatgct
     2161 gaaaggaaaa caccaacatt atgttttgct ttttcagcta gaacgagaca agcaggaata
     2221 cctttagaag atatcaaagg ttttttaggt cataaagatg tttccactac acaagtctat
     2281 gcccatattt ctcccgaagt gaaaaaaaga tcaatgaatc aactagaaaa ctatatagaa
     2341 gagcaaataa aaaagcactc aaattgagtg cttttttaag ctcactatca ctaaaactat
     2401 cactaatttt atttataagc gaacatgttt ctatcactga ttttttaaat ctagcgaaaa
     2461 acccttatct taaaggcgtt cgttcaattc tttggcaagg tcttcaaagc ctggcttgcc
     2521 tagaagtgcg aacatggttt tcttatagct catgtagaaa aattttcttg atgatgattt
     2581 tatccatcaa ttgagattga ctgttttgct ctcctatgat ttcagctatg attgtccgat
     2641 tttgcggttt tcttcttaac tggtagttga ttttcccagt ttccatcaac tcgctaagca
     2701 tatccacgac tccgccaaat ccggtctttt tcgtaataaa acctagtgct accattcgtt
     2761 catctagatt ttttgctgta atcgtcacac cagtaatgag cgagtgctgt aatgtttggg
     2821 ccagctcttc catcccttca gcattggata cgacggaagc tgttttgtta ataggttctt
     2881 cgtttatttc ttttggttct ttcctctcca ctttcactgt ttgacgaagg acatctagtt
     2941 cttgcaagag cgtttcgtat tgttcttggg tcagcatcac gccagctact ttttcacgat
     3001 taaatatata tacgccagtt cccgcgtctc gcgcttgagc aaatacatcc atcggcgatc
     3061 gtttcacttc tgtgatcgaa gatgtgggaa cttctaattt ctttaattcc aatggatttc
     3121 ctcctttact aatcgctttt tattatagca cagactgact tgttttgatc ttttttccaa
     3181 aggttttatc ggtttttgcc taaccagtaa ttgatatcgg taagaagtct ttcgatacca
     3241 aatgaaggat ggtacagata cgttttttga tcttcgttat ggtctagtgg aaaaaaatga
     3301 tcgatgatca ctagatctgc ataccgaggc ggcttatttc gagtacatac atgtatagag
     3361 atccgttttc gatgtcttag cgttttgttc agtaccccag caagtcttct tgcttcttgg
     3421 tgttcatagc gaaaaaaaaa gacgattttc acttcaggag ggtggacatc taaataccct
     3481 cgttcctgga gtgttttcag taaaagcatc ccccactcta ctgcggggtg tgcaagtgct
     3541 tgttggatcg ctagccgaaa ttgatatagt ttatctttgg aattgggaaa tttttctaat
     3601 gtttcctgat aataacgaac ttcttctatc agccggtcat ggataccaat tacgtaacga
     3661 agtcattctt ggtaaagtac ttttgtcagc tttgatttgc tttcgctctc aaattcgcta
     3721 ttcagccaaa acagcgaatc aagcttttca gtcacaatcg tcaatttccg atagtcttgt
     3781 gtctcttcta tccgtttcca ccgcttttca gtaatctggt tacagctcaa gcgcaaaatt
     3841 gctagataat ttttttcttt tattggcaaa atctgctcct ctctaaatag caattcagga
     3901 aacaattcaa agaatagtgt tcgactgtct cctaaaatct cgatttctgc cggatccttt
     3961 ctttgcttca ataattggat gaacatctca gaaaaggact gctctccatc atctgcaaaa
     4021 aaatgcggaa aatctttcaa aaagcaaaac agcagatatc ttacttttct ttctgaacca
     4081 aagatctgtg cttttttata agaaaaagaa aggtggtact cgccaaacca ctttctcaat
     4141 cgctcttgcg tagaacgatc gtacttctgc tcgtaaggtt cattcaatag cagatttttc
     4201 agtaaacaaa acgggggtga ttggatcaga aaaacaaaca agagataagg ttcgctatat
     4261 ccttgatcat aaactaactg aacggttttc ttatgaagaa tcagctgaat gggacagtcg
     4321 ctttccgcca aacgtttcag atgccgatcc actgttttaa catcgatttt cagttcttca
     4381 gctaaatcag cttttcgcca gatcaaatct ttcattagat tgattagtga aaataaattc
     4441 tgtgcatttt ttgataatag tttttgccat ctcatcgatc atttctcacc tgatttctaa
     4501 acatattgtt tgatcaaaat gggttcactc ctaaaaagat aaaaaactcc ctttctattt
     4561 atttaaatac gcaaatcttc ctcattattt tttattttac tcaatttaat cgtattctca
     4621 tttcaaaacg aatgtacttt aaaaaaacat aataagatca ctttgcttgt aggatgtatt
     4681 cccttccatt acaatgaaat aaatagcgat tttatgagaa ggtgatgtga aaatggaaga
     4741 aaaaatagaa gaatccaaac aaaaaatcga tcagttgata taagaattag aaaaagcggt
     4801 catcggcaaa cgttcaacta tccaattgat gatcgtggcc ctattagcag gtggtcatgt
     4861 cttattcgaa gatattcccg gtgtaggtaa aacgttactg gtcaaagcac tagcaaaagc
     4921 tgtacaggga accttcagca gagtccaatg tactcctgat ctgctgccta gtgatattct
     4981 tggtttttcc gtatacaatt cacaatcaaa agagtttgaa tttcgtccgg gaccgatctt
     5041 taccacgatc ttgttagcag atgagatcaa tcgtacgact cctcgtactc agtcagcact
     5101 tttagaagca atggccgaga atcatgcaac gatcgacaac aatacatacc ctctagacaa
     5161 tcattttttt gtcttagcta cgcaaaatcc aatcgagtac gaaggaacat atcctttgcc
     5221 ggaagcacag ctcgatcgtt ttttattccg actacaaatc ggttatcctt cttttgatga
     5281 cgaacttttg ctacttttgg ataaattcga aaaagtgttg gataacttaa atgaagtatt
     5341 gaacagttat gaaatagaag aaataaaaac aaatgtggcg aatgtctttg tcagtcccga
     5401 agtggcatct tatgccttgc agttagtgtc agcaacaaga agtcatgctg ccatccagct
     5461 tggcatcagt ccacgaggaa gcctttcctt tattcaggca gcaaaagctt atgctttaat
     5521 ccatgggaga aattacgtca cgcccaaaga tttgcaggat ttagttcctt acgtgttttc
     5581 ccatcgcctg attttttacg atcgttcatt gaaggaagac gaaaagaaac aaatcattca
     5641 tactcttgtt gcacaaattc ctattcctgt gaggtagatt gaggtagaca atggaaaaaa
     5701 tcaaagatta tttcattaat tttgtgttat ttctggtata tttcatcatt gtgctttttg
     5761 ctcttgtatt caacaattct ttaggctggt tcttgttggc cttttttact ctttatgtat
     5821 catggagctt gatttcatta agcagctttc taaagaaact ttcggttact tgtacattgc
     5881 agccactcgt ttatcgcaaa aaaagtatgc aggttatttt ttccatagca aaaaagagaa
     5941 ctttttcttt acctctccct cgtttacaaa tcggcttccc agcaatcttt gcagaaaaaa
     6001 acaagagatt ttgattctta caaataaacc gcaagaagtc caattgcttt ggcagccaaa
     6061 agaaagagga tgccatgaaa cattaactgt catttttaca gcctatgata gtctaggact
     6121 tttcagaaaa agtgcaaaaa gagagatctc ttttcctgtg actgttctgc ctgctttttg
     6181 taagattcaa gcagaaaaat tacagagagt tctggaaaaa aaacagcagc tgaacgcgta
     6241 ccaaagaggt cttgattttc gggaacatcg cgcttatcgt ccaggtgatt ctttcaaccg
     6301 aatcgattgg aaattgtctg ctcatactca agagtggtta taccgtgaat acgattatca
     6361 agaagaagaa tgttctcctc ttttttgctt ttggggttcg cccagtccaa aatttgaacc
     6421 tctgctagac ttgtattttt ctttatggca tttgagaaaa gaaaagcagc cggaacttct
     6481 gatattaggc aaccgcgcct ttcatggcaa agaccccggt catacctatt ttgcttcttt
     6541 ggaagctggc gatgagaaag cttttttcgc acacttgaaa aaatatgcaa aaaaacaaat
     6601 cgtcctcttt gttcctgaga attctcctga agtctcagaa gctattgaaa cattgtcaaa
     6661 agatcgaacg gtttatcttg tttatttcgt agaaaaacaa ctcatggtcc aagaaaaaga
     6721 caagatatgt tcattgccag gaggtgaatg gattgacgat taaacgggaa aaatggctgc
     6781 gtggcgttta ctcctttgtg atactaagtg taggtgctcc gatatttgta gtagctaatc
     6841 aattgcaaaa cagctacctt cttatcctaa ctattggtgt cctatgcctt attgtctgct
     6901 gtattcctaa aaaatgggta cgtttgctgc tttctttgcc agtaatgata ggttgtttgt
     6961 ttttatattt tccttcgaag acattttcca tattatggat cttacagttt ttttcgtcag
     7021 tcacagagga attcttccat ctatcccaag gtaaacttta ttatttgcca gaaaagacag
     7081 ctttttttct cataatgttg ctgatctatt tatttattac attgaccgtg gattacgact
     7141 attggtacgc accctttctt tctttgatgg cttacttcat gatgcttacc gtttttcgga
     7201 aaaaagattt actgtacgaa atgattgcgg tagtaactgt tttgttcgtg tatctggcag
     7261 caaggcaatt tttctccatc cggttgttgt cgggaaacac tcgattccag tcattgacta
     7321 cagttctgct tatttgtttc ttaactttta gcgctatctt gcctttatat cttccgaaag
     7381 tacaccaatc tctcgaggaa acgagtgaac cgatccgaga atatcttaat gatgaagggc
     7441 tttatgacac actccatgag tatcaattaa ccggaagtgc aagaacaggc ttcagtgaga
     7501 atgatgaaca gcttggtgga cctttatatg ataatggcga agttgttttt acagcgaacc
     7561 aaaaaggaaa tggctattgg aagatcgaaa ataaaaatca ttacaccggt tctggatgga
     7621 tagaagatca ggacgcaccc atccaaccaa tccaagaatt ccctgttgac atcacgagct
     7681 accaaaccgt tatagaagac aaagaaaaca ttacattgac cctaaaaaaa caagttttct
     7741 taccttatcc ttatggaaat actaccttga ctgatttttc attagctagt ggacaaacgc
     7801 tttactattt gcctgaaagc cagcgattca ttcctgtccc gaatgaatcg acacgttctg
     7861 caactttaca aacagaaagg ttagttttta cgagagaaga attaaaaagc agtcagttaa
     7921 aagctgatga tttagaaaat cagcagacaa atcttcaatt gccaaaaagt ttgcctaaac
     7981 gtattgagga cttggcggtc gaattgacca aaaatgaagc aactttaatt cgtaaagtcg
     8041 aagctgtcta acagtattta aaaacaagcg gaaatttccg ctattccaaa acggataccg
     8101 ggcatactcc aaaagaacac gattatgtgg attatttcct ctttgattca aaagtcggct
     8161 attgtgacaa cttttcaact tctatggtcg tccttcttcg aacacttggt atcccagctc
     8221 gctggacaaa aggttttaca aatgggctca gaacaggcac agataatcaa gggaatgagg
     8281 aatacagcat tctcaatagt catgctcatt cttggccaga ggtatacttt cctggatttg
     8341 gctggcttcc atttgaacca accccttcat ttgccaatcc aatgggttca caaccagttg
     8401 ctgaaacaac cgaaaccgtc cagcctgcta tcgaacaacc aaatgaaagc gaaaatatgg
     8461 cagaaactat ttccagtgag agatctgagg tcattccatc agaagaaaca acactcacac
     8521 cagcttataa aatcgtctta ttcagccttg gagtgtgttt atccgtcttg ttgttctttc
     8581 tattgttatt cagaaaatct ctgcgctgga caagttatct tatctatctt catttttttg
     8641 cttctactaa tacgattgca tatcaaaaac ttcttaaccc tttggaaaca ttggtaccgc
     8701 gcaaacccgc agaatctttg caggaatata gcctgcgaat agaacaagaa ttcaagctag
     8761 atctgcgttt cattcgatta actactcaat atgaagcaga aatatatgga cgacaagcag
     8821 atccggtaaa attcgataaa aaaacctatt tagaaataac gcatataata agaaaaatgg
     8881 tatacaaaaa aattctgaca aaagggagcc gcttcaaaaa ataagataaa atttctggag
     8941 aatctacttc tggaatgccg agaaccagct gattgagtat cgattactcg aaaaaaaggt
     9001 tgtgacacgt tttttgtcac aacctttttt cattattatc tgattttcaa tgtcaaagca
     9061 tttagagcta caactaccgt acttaacgac atcaagacag caccaactgc tggacttaat
     9121 atgataccaa ttggcgcaag aattccggct gctagcggaa ttgcaagaat attgtaacct
     9181 gccccccacc agaggttctg gatcattttc tttcgcgttt gcttggctag atcaagaaaa
     9241 tgcaaaatat cttttggatc gctgtttgtc aatacgacat ccgcagaatc gattgcgata
     9301 tctgtccctg caccgattgc catcccgatt gaagcacggg ctaaacttgg cgcatcattg
     9361 atcccatcac ccaccatgat tacttttttc ccttggtcaa ggtatttctg gacaacagct
     9421 tctttatcat ctggtaataa accgccataa tattcttcga tccctaaata atctgctact
     9481 gcttgggcag cgtttttatt gtcaccagta agcatgacag gagtgatttt tctttctttc
     9541 aatgttcgga taaattcttt tgcttctgtt tttactacgt cacctaaagc aacgattgca
     9601 gctagatgat tttctaaaat caggaagctg atggtatttc cttgttcttg gtaaggtttc
     9661 aataatgctt gttctacgtt taatccgaga cgctcaactt ctttttcatt aactattttc
     9721 acttgttgtt ttttgactgt tgcctctagc ccgactcctg aaagattttt tagattttca
     9781 gcttgataag gcttgatttc atgttctttc aaataattca taatgccaac agccaatgga
     9841 tgattggcat tcgcttccaa ggctcctaga tattgaagga tttcattttt attgaacgca
     9901 tcatcaagta cttcgacacc cgttacagta aattttcctt ctgttagtgt tcctgttttg
     9961 tccagcatga tcacatctag atcattggct tgctccagtg cattgcggtt tttcaaaagt
    10021 aatccgtttt tagctgctat tgatgtggaa cgagcaacaa ctaatgggat agctaaacct
    10081 agagcatgag gacaggcgat gataaagact gttaccatac gttctaatgc attaggaaga
    10141 tctgtcaaga acaaccaggc gatgaatgcc aggataccca caattaatgc gacataaaac
    10201 agccattttg ccactttgtc agataatgac tctagcttgg atttttctcc ttgtgctttt
    10261 cgcaccattt ccataacctt cgctagataa ccattttcac cagttccagt tacttcgatt
    10321 tcgatcgttc catccccatt gatagaaccg ccaatcactt tgtcattggc ttgttttttg
    10381 attcctcttg attcgccagt cacagctgat tcgtcaacaa ttgtttcacc tttcaagatt
    10441 acaccatctg ttggcatttt atcaccagac cggacgatca aacgatcacc ttcgtttact
    10501 tctttcaatg aaactgtctc ttcttttcca tctttcgtca agcgtttcac tgattctggc
    10561 agtaattctg ctagtttttg taaagcattg ctggcattcg acacagcatt catttcaatc
    10621 cagtgaccta gtagcataat aacgatcaaa gtagccaatt cccagaagaa atccatgacg
    10681 tgtgtatgtg gattgagcag atttgcgata aacgaataga cactataaat atatgcaact
    10741 gtaatcccca tcgcgatcag tgtcatcata gcaggacttt tttgtttcaa ttccatctta
    10801 gctccactta aaaatggctg accgccatag ataaatagta tagttgctaa gatcaatacg
    10861 agccaatcag aaccaggaaa cgttacttga aatggaaatt ccattcccat cattggtgaa
    10921 aaaagaatga ttgggatagc taggataagc gatagccaaa atttttgttt gaaattcccc
    10981 atgtgcatgg aatgatccat gccgccgtgt tcgtgatcca tgtgcatcga atggtccatt
    11041 tgtttgctgt ttgctatatc tccatggtta tgtcccatgt gcatggaatg atccatctga
    11101 ctatggtcat gtttcatctc catctgtttt tcatgagatt ctgtactttc tttgtttact
    11161 acttcttttt tatgctcatg ttttttctcc atatcgttcc cattcatttt tactccatcc
    11221 cttctcttac tttttcatgt gtcgatttaa gcacagggag ttcaacaata cactgacgga
    11281 actaaacgcc atcgctcctc ctgcaatgat tggattcaaa aagcctagtg cggcaaaagg
    11341 aataccaatc gtattataga caaaagccca gaaaagattt tgtttgattt ttctcaatgt
    11401 caacctagac aactcgattg tttgtgcaat ggatgccaaa ctgctattca tcagcgtcac
    11461 atcggctgtt tccatcgcaa tgtccgttcc acttcccatt gccataccga catcagctaa
    11521 tgctaaggcc ggtgcatcat tgattccatc gcctgccata ccgacttttt tgccatcttt
    11581 ctgaagtttt tctacgtagg ctgctttatc ctctggaagt acttcagcaa agatatgctt
    11641 tggatcaata ccaacttgtt taccaatcgt ttcggctgcc agtttgttgt ctcctgtcag
    11701 cataaaaaca tctaatcctt tattctggag ctgcttgatt gcttgtttga cttctagttt
    11761 gatttgatca gccacggcaa tcaacccgat cacttgcttt tcatcagcta aaaacatgac
    11821 tgtttttccc tgctgttcta attctaacgc gtgttcttgg tattcatcaa atgataggtt
    11881 caattcaatt aatcgtttac gtgtacccgc aaaatagcgt actccgttaa tcgtaccgct
    11941 gattccagca cctgggtggg ctgtaaaatc aatcatatcg tatgttgctg cttcttgcag
    12001 ttttccgtat tccacgattg ctttacctag tggatgttca gaagcttgtt ctaaagtata
    12061 aaaaatagat aggacttctt tagaaccgac tagatctgta actttgggct ttccttctgt
    12121 aatcgttcca gttttatcca aaacaatgct gtctaagtga gcagctgctt ctaatgcttc
    12181 tccgccttta attaaaattc catttcgtgc accaacaccc gttcctacca taatagctgt
    12241 tggagtcgct aaacctaatg cacatggaca cgcaatgact aatacagaga cactatggat
    12301 caatgcgagt tgccaatcac cagtgatcaa acctgtagct atgagcgtga taaacgcgat
    12361 gactaatacg atcggaacaa aaatccctga gatccgatct gcgatttttt ggattggggc
    12421 tttgctccct tgagcatctt ccaccatttg aatgatttgc gctaatactg tctgcttgcc
    12481 gacttgtgta acttccatgt gaagtagacc attcgtattg attgtcccgc caaagagcgt
    12541 gtcatccgga ttcttttcta caggaagatt ttcaccagta agcatacttt catctatcgc
    12601 actgcttcct gaaataattc gaccatctgc tggaatctgc tcaccaggac ggatgactag
    12661 ttgatcacca acgactactt cttcaatcgc taacgtttct tctttcccgt ttcttatcac
    12721 ttgggcggtt tttgtttgaa gagacatcaa ttgtttgatt gcattacctg ttttcgtttt
    12781 tgctgtatgt tccaaatatt tgccgagcaa aatcagcgtg atgatcatac tgctgctttc
    12841 aaaataaaga tcttgggggt gtccacggaa aaatccgttg taaatgctca atgcaaaggc
    12901 agccgatgtt ccaattgcta ccaatacatc catatttggt gctttcgtct tgattgcatg
    12961 gtacgcacct ttataaaaac gagcaccgac ataaaattgg acaggagcag acagcactaa
    13021 ctggacgatt ggaaaatgaa aaaagtgaac aatcgccgca tggcttccta acatcatagc
    13081 aatcattgac aacatcaacg gcaaagtcaa aaccgtactc aatataagat cgaatagcat
    13141 ttttttcaaa tacgcttgct tttcttctgc aattttttgt ttgtgggctt catcgtacaa
    13201 aattgcacca tagccgatgt tttctacgct ttggattaat ttttcagtgg tcgtcccttc
    13261 atatttgaca cttgcttttt cagtggccaa gttgactgtg gcattgacca caccaggttg
    13321 tccgttcagc tctttttcga tcctagcaga acaatttgca caagtcatac ctgtaatgac
    13381 gaacgtatcc attttggttt ctgctgttgc catcaaatca cctctgcttt ataacccaat
    13441 ccattgattg cttgacaaat cttttcggat tgaacttttg tttcatcaaa cttgaccgta
    13501 ccgtttgctt ttttcaaatt tactttcact ttttgcacgc cgtcaagtgc gcttactgtt
    13561 tcttctactc ttgctgcaca atgattgcag ctcatacctt cgattgaaaa ttgttgtttc
    13621 attttttctt cctcctattg atgttgttta cattcacatt gacctgggat acagttacat
    13681 tcgatcgtat ctactggttg cttttgttcc agttgtttca aaatctcatt gatatcttct
    13741 tgagtcaaag tggcttcttc tactagatcc gcaatcgtct ctccaatcct ttttgcacag
    13801 atatgggaaa aaagattttc cgtcgcactg cgaactgttt ccatctccga gacagctgga
    13861 taataaatga atttttttcc ttcttgttct gtccagatca cttctttttt taccaaacga
    13921 cccagcaaag ttttgatcgt tgctggtttc caatccatcg aatcagctaa gatctgcgtg
    13981 atttcttggg ctgtcgtctg tccaagtgtc caaataacac gcatgatctc ccactctgaa
    14041 tcactgatct ttactggcgt tttgactttt tccatttatt tcacaccttt cgtttacaaa
    14101 tgtaatcatt atcttatgaa tacagattac acttgtaaac aaaaactgtc aattattttt
    14161 gctctctatt ttcaatttag ttaaaaattc ttgtttctct cgtcttgttt taaggatgat
    14221 cagtttattt cttgcattag ctacttggat cagctgctgt attttcggtt cattgttttc
    14281 ttcaaaatgc cagataaaag ataaaaattc taagtattcc ctgtttagtt tctccgaaaa
    14341 tgattcaggc atatctggcc gagatttttt gtttatcttt ctttttagag aacgccaaac
    14401 tactcggtag atggcgagat aacgcggcac tttaagccaa atgatcttat ctgcttcttt
    14461 aatcctttcc tctaatgtag aagtataatt tccttcaacg atccatgacg gattagatac
    14521 cataaacaat tgctgttctt gcaaaaacca cttttttgcc tgaatactgt agtctgttgt
    14581 atgccataaa aggtctagcg gcagtagcgg aaagtcggtg atttctttga tcttccttgc
    14641 gagggtcgat tttcccgacc cactttgtcc aatcacgatg atcttcattc ttctccctca
    14701 cttctctttg gaatttggtt cgatcatacc acttataacg ccgcaaaaaa acaagggact
    14761 gtgacaaaag tcgtgtcaaa gtcccttgtt tcgaataa
//
LOCUS       DANKEF010000057        14682 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000057 DANKEF010000000
VERSION     DANKEF010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14682
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.057"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(5..1078)
                     /locus_tag="PWZ48_002154"
     CDS             complement(5..1078)
                     /locus_tag="PWZ48_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261802.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            1300..1974
                     /locus_tag="PWZ48_002155"
     CDS             1300..1974
                     /locus_tag="PWZ48_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2261803.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            1979..3319
                     /locus_tag="PWZ48_002156"
     CDS             1979..3319
                     /locus_tag="PWZ48_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2261804.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(3468..4454)
                     /locus_tag="PWZ48_002157"
     CDS             complement(3468..4454)
                     /locus_tag="PWZ48_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HDL2261805.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            5178..6209
                     /locus_tag="PWZ48_002158"
     CDS             5178..6209
                     /locus_tag="PWZ48_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2261806.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            6318..7850
                     /locus_tag="PWZ48_002159"
     CDS             6318..7850
                     /locus_tag="PWZ48_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDL2261807.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            7989..8801
                     /locus_tag="PWZ48_002160"
     CDS             7989..8801
                     /locus_tag="PWZ48_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HDL2261808.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            8791..9276
                     /locus_tag="PWZ48_002161"
     CDS             8791..9276
                     /locus_tag="PWZ48_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDL2261809.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            9299..9844
                     /locus_tag="PWZ48_002162"
     CDS             9299..9844
                     /locus_tag="PWZ48_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HDL2261810.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(9917..11560)
                     /locus_tag="PWZ48_002163"
     CDS             complement(9917..11560)
                     /locus_tag="PWZ48_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDL2261811.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(11689..13917)
                     /locus_tag="PWZ48_002164"
     CDS             complement(11689..13917)
                     /locus_tag="PWZ48_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2261812.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(13950..14429)
                     /locus_tag="PWZ48_002165"
     CDS             complement(13950..14429)
                     /locus_tag="PWZ48_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261813.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT         4354 a         2794 c         2674 g         4860 t
ORIGIN      
        1 tttattaacc tcctattgct gtaattgggt ctactttggt tatcgtacgt atcgaaaaca
       61 atccgcctag cattgcgaca atgatcaaaa tcccactata tagcaaccat tgtgaccaaa
      121 aaacagcaaa aggcatcgcg cttggtaaaa tcagactagt caaataagca aacaaaagag
      181 caagcagaac gcctaccacc ccaacaataa aggactgtgc tactaaagat ttagcgatga
      241 agaaattttt gatcccttgt gctttcatta ctccaaaaat agcagtcttc tgaagtgtca
      301 tcacgtacat gaaaataccg accacggcag caaccactaa gaacaaaaag tatatcatcg
      361 catttaaggt catattttgg gcagaatagc caggaatact ttcaatgaat gtcccaatag
      421 ttaataactg tagacccttc gatgcttttt ctttcgaaat tttagctgaa tcctttatta
      481 cgatcccatt gatcggctgt tcttcttcag aagcaaacgg ctgttgtcca tacttcaaag
      541 cagtccacgt atctagggac gcatagatga cgggagaaac tgtgtaataa gtttcaggaa
      601 agataccgac aatcgtcaaa ggatcgtcgt aactgccaat tttgatcgta tctccaattt
      661 tatagcctga atcagccaga ttttgagaga tgatgatttc attctttgtt ttaaattcat
      721 gccctttcgt gagttttggt aataaaaatg cttctttcgt tgtgccgaaa actgtaacat
      781 tttctttgtt ttttcctttg attgctccgc tgtacaatcc gattggtgct ttctctcctc
      841 cttcaatgta tttcagatct cctcttgtta attgtgaagc tgcaaatact tgatttgcat
      901 cctccgacaa aacaatctct tgtgcgtccc aatcatcgat tgctttttta aactcttctg
      961 aaagtccatt cgctagtcct gacaacatga atacgacata cgcaatcaag aacataatac
     1021 cgacgatcaa gccatatcgt aatttagaat agcgaatttc tttgatagct agaaacattt
     1081 gtttttcctc cctatgatag attgatagtg acaaaatgtc acttgttgaa tagtgtaact
     1141 ctaaagtgac aatttgtcac ttgtgttgtt tttttcttat caataaattg tctaagacat
     1201 ctttcgataa aaccttaaca tgagtcataa aaaatttatt taagccttat ttatgctttc
     1261 catgataaac taaagtgacc aaatcaatga ggtgggaaaa tgattaaact attagtagta
     1321 gaagacgata aagtattatc caataatata aaagaaatac tcgctgcaat cggcgagata
     1381 acacaagttt atgatggtag tgaaggactg ttcgagttgg aaagtggagt ttatgatctt
     1441 gcaatcttgg atttgatgat gccggaaatg aatggatatg aagtgttagc aaaaatacgg
     1501 aaagaaaaaa tacagactcc agtattgatt ttgactgcaa aagatagttt ggaagataag
     1561 attgaaggat ttgaacgagg tgcagatgat tatctaacaa aacctttcta tcgagaagaa
     1621 ctgatcatgc gcgtgaaagc attattgaaa cgctcgctag gtctctttga tgaaaatgtt
     1681 ctgaagtata aggatatcag ttgtgattta aaaagcaatg aagtcttgta taaagaacaa
     1741 gttttgccca tacaaggaaa agagtttgaa ttgctcgttt attttttaca aaataaggga
     1801 atcattttga caaaagagca gatatttgac cggatctggg gatttgattc tgatacgacg
     1861 attacagtta ttgaagttta tatgagcaac ttacgaaaac atctgcgaag tactggattg
     1921 gataaagaaa tcaaaacctt acgtaatgtc ggttatatac tgcaaagggt gtgatgcaat
     1981 gaatggagat attacacgaa agcaacgtag tcgctttttt gcgacaaatg tagcagcatt
     2041 tgcttttatc ttttttcttt tgggattcat tactttacag atattaaacc aatctgcata
     2101 cagagaaaca gatctgtcat tagagaaaat aaattttgat tctcgaacag tacaattaga
     2161 gatacaaaga tatcagcaag gcgatccctt tttaaacccg actcaaacgg aacaaaacag
     2221 aacaaacatg gaacagccat taatggaacc atcaaatgac cgttttaaca ctcaagtgat
     2281 cttgtggtct aaaaatggtg agatattgaa taaagcagct attggtgggc gtttcaacca
     2341 aatcaaagaa ttaaaaatgg atacgaaaaa tctgaatacc atccaaaata ttgaattaaa
     2401 tgaagacagt ggctcatctg ccctctcttt ccgttcgatt acgaaaaaag cggagacgaa
     2461 tcaagatggc gttgcctaca tccaaatttt agaaaataca aatcaaatca agaattcatt
     2521 agcgactttt cgcacgatta tgattttgtg tatgattgtt ttttggattc tctctattgg
     2581 tatcagttat tacttgtcta gtctaagcat gcgccctatt attctttctt ggagaagaca
     2641 aaaagaattt gttgagaatg cttctcatga attgcgcaca ccgttaacga ttattcaaaa
     2701 tagtttggaa catcttttta ccaagcctaa tcatactatt attgaagaat ccgaaagcat
     2761 cgctcaagca ttgtcagaga cgaggcgcct cactggattg acttcggatt tgctgacaat
     2821 cgctcggaat gattctgatc agcagctgct ttcaaagcaa atgatcaata cacaagaata
     2881 tatcgaacaa cttgttaaac catttcaaga gatggccatc atagatggaa aagagtttat
     2941 tctccataac gatgcaacat tagaagtacc aattgatgaa aaaaaaatcc atcaagtttt
     3001 ggttattttg ctggataatg cattaaaata tacgaatcca gaagataaga tcattttgga
     3061 ttcatctgtt aaccataaag aatggctgat tcacgtaaag aatactggcc cttctatcag
     3121 tgaagaagat caagcaagga tttttgatcg attttatcgg gaagatcaat ctcgggcgaa
     3181 agagactggc ggatacgggc ttggcttggc aattgcaaaa caggtaatcg aacagcatca
     3241 tggacagatt tctgtatcca attatcttcc acaaggagtc gattttaaag tccaattgcc
     3301 gttgaagcct aaaaaataat gcttatccct aaaaaagggc tgtgacaaaa atcttgtcac
     3361 agcccttttt tgttgcgaat aaacggtgtt ccagaagtag cttcttcgga aataagccga
     3421 aatttcttta agctgaccac ttctgtcaca acctctttag cttacgctta cgatttttta
     3481 ttgcggtctt caatgtgtac atcaccgctt gtgtcaatat ccaattcttc tcggctaaca
     3541 tcttctgtca ctttttcaac atcttcatgt tcttctttat gaatcccaac ttcttcacga
     3601 ataactgtat gtttgcttac atctacttgc tcttctttga ttgggatagt aatcgtgtca
     3661 tcttccaatt tttcgtctgt tccctgtgaa gattgatctg ttactggttt gcgttcgatg
     3721 acgatttctt cacgtttgac cggtacttct accgtttcag tatcgttgac cacatgtttg
     3781 tggatgtcga cttctccagt tgtcacatca tgtgtattta catctaattt ttcttctttt
     3841 agttgaattg tatcatcatc tagatttggt ttgcgatcga tgtcttcagt cttgcttgta
     3901 cccattgtat cagtctcgcc aggtacgtcc atcggatcag ttcctggtgt catattcaat
     3961 ggatccgttg tattttgaaa gcctgtcgtg ccacctacgt ccattggatc tgttccagtg
     4021 ccagtttcta atccagtccc cattgtgctt gctgggctaa cagtatcttg tgtatccatt
     4081 gtatcttctg ttgtttctgg ctgacgatag ccttttactg tgatgacaaa ttttccatca
     4141 gaaatatctc ctctatattg atataaagga tcattttctg gtgtagccgt ctcagaatca
     4201 taggtatcaa aagaaaaagt atcttttatt ttttcccata aagaacggtc ttcttcacca
     4261 tccatccgat tttcatctgt ttcaacatct ataccagaca atccgtcaaa tcctagtcta
     4321 gatgctgctt catcagtagt ataaataacg atatcatctc gttcataccc ttcgttacgc
     4381 aaccgttcaa ttactgataa tgcttcttcc ctcgtactat agcttccgat gaccgttaaa
     4441 tttttctctt ccatgtaaat tcctcctagc aaatagattt atgattggta tattgttact
     4501 tataaattta ttctatagga gacgattgtt ttctacaaat atttagattg gaatttgatt
     4561 aaaacaattt aattgaaatt tgaaaacatt tatgttaatg aaagtcatat tctaataaaa
     4621 tgtcatttgt ttctatatga atttttatga aaaatttcat ataatttcca ctcgttgtga
     4681 tggtttaatt tatccaatcc atcaataagc aaacaactta tataaaaaag gattaattct
     4741 caatataatt gctcctacca tagaataaat cttcatgtaa agtaatgaag taccaactat
     4801 ttctaatact tctttaagaa ttatcccaac aatatcgtta gacaaaccat tattgaataa
     4861 atggagagcc aatcgattct gaaaaaatgt tttctaattt ttcggaaata aaagaaagag
     4921 cctcctgcca ttagaacgaa ccaaatccat ttcattgctc atgaagtaac cacatagcta
     4981 aaaagaggat aaatcaatta aaagatcaaa agaatgactg cactaataaa ataactaaaa
     5041 atgtatcttc ttacttcata tcttttagct agtacttagt taaaaatttg cttcctaaga
     5101 tcaagacaaa ctgtctatct tcatttgatg aaatgcggta aaatatagaa aatgaacact
     5161 attggaggga atttgtaatg gacagcaaaa ctagaaaaca actgcaagaa aagttaaagc
     5221 aacgtacaca cttgctccct tccgtctttc aacagttttt agaagaaaat cgttcttccc
     5281 tttcattagg cagtcgctat gaatacgcaa aagattttca tctgtttact gattatttga
     5341 gaaaagtatt ttctgaagaa gtggatgatc agctgattat ccatttagaa aaacaagatt
     5401 atcagaattt cttagctaaa atctatcgac atactcgttc gttccaaact acagcgaacc
     5461 aagaaacgga gcaattgttt gaaaatagct attacgggat ttcaaggaag caatattcat
     5521 taaatcattt tttacgattt ttgtatcaaa aaggactgat tgaagaagag atttctcttt
     5581 tgggtacaag tttaccggaa acaaccaaag cagctccgcg atatatcgat gcagaacttt
     5641 tcaaagattt tgagcatttg tttatcccga ttgaaggctt tcaaacaagc agagaagcta
     5701 gctttgaaga aaagaataga ccgaggaacc ttgcaattac tgccattctt ctgtattgcg
     5761 gattaaagat ccatgaagta gtagaactta aacgtaaaga tgtatctttg accaagcaga
     5821 ttcttaatct gaatagaaaa gaaaacagac tgaataaagt accaatacca aaagaagcga
     5881 tgccatttct ggagaactat tttaagttaa cagcacaaaa tacggacgaa aattcttttg
     5941 tttttcgttc ctcacatgat cagcaaattt ctcctcggac tattcgccaa attttgagca
     6001 aaattacagt atataaaaac gtatcgccag aactatgcag aaaaacatat cgttattatg
     6061 cagaactata tttagatgat gctgacgcgg taaattattt atgtggtaat cgatacctct
     6121 catcccattc ttttcaagat atctgggaaa agatggttga tttttcttat cacgaattag
     6181 cggcattcgt tcgggtttct tccatataaa acaaatgtat gatctttttt tcacaataaa
     6241 ctatgttatt atataaagga ttatgttacg attatcttga atttgtatat tacttaacaa
     6301 aagataatgg agtgtgaatg atggagacca taacgtattc ccctttgaac ttatttacga
     6361 attttcaaga agctgcagct aacttttccg agaccccgat tatctttgat gagccacttt
     6421 cagctttccc agaattagga cttgaaacaa cttacaaaaa atgctgcgaa agtgtgttga
     6481 aacgggctta tcaattggct catcttggag tgaaatccgg agacaaagtt attatttata
     6541 agagttccgc ttttgacaca taccttctag ctgtttctgt gtcttacctt ggtgcagtgc
     6601 ctgtcatgac ttcttatcat ctaccaacaa caacaatgga agtatttatc gatcgtttag
     6661 aagacccttt cattttattt gatgatgaga caaaaaagcg agtgagagaa atttcaaatg
     6721 gaacaaaatc aaaacagatt cctattcttt atttaatgga acaacctgct ttgtctgttt
     6781 ctcaatcatt tttagacaaa aatgaaattt cttatatgac ccatacttct ggtacaactg
     6841 gtatccctaa acttatttgt cattctgctc attctatggg atggcggacg aaatggcaaa
     6901 aaacagtttt cacaaaaatt tcggaaaaga aattaatcgc ttttcacatc tctccagtgc
     6961 attccagatt caatattggt atctcttctt tgatgtccat gggatttcct atgatgcctt
     7021 tagcaaacgc acaaagttca aaagttgttc atatgttgga agctcaccgg ccaattgcac
     7081 tagagaccca tccgaataac tttgttcagt ggcgttttac tgctaaggag catccagaag
     7141 ctttttcggg tatccgatac tatcactcca cttttgatgc tataaacaac cagacaatga
     7201 agacattttt gcgaacgtcg atggcacagg atgcggtatt tcttcaagta tatggccaaa
     7261 gtgaatgcgg accaatgatt cttaaggctc atacactaga gagtttgaaa acaagtgatg
     7321 caagagatat gggcgttgga ctggaagaca tgacaagcgc tcggattaca gattcagttg
     7381 ggaatgtcct acctgaaaac acagatggtc atatacaatt actgtctaaa ggacgggctt
     7441 tgacttatta caaggaagat gcacggttcc aggaaaatgt ttatggcgat tggtgggata
     7501 gcggagatta cggttttatg gataagcatg gccacctatt tttgaaagat cggcaagtgg
     7561 atttgatcga aacgatcaat agtacattag ctattgaaga ctttttatta gattcgttag
     7621 attttttggc agaagtagtg attgtaaggg ataaaaatca ttctccacaa ccgattattg
     7681 ctttagctcc tgataaagaa atggattgga atcgatggtg ggagcaagtc catgaacttc
     7741 ctcgcttgaa cgtccctatc atccgtgatt ttgatgcaat cccgcgaaca gctacaatga
     7801 aagtgcaacg gcttcaaatc gagaaagaat taaaatcaca aaagtcctaa ctatttaagg
     7861 aaagaaagat ccattttggt ctttcttttc tttttgtttt tttaaattgg atgagataaa
     7921 aatatgccaa ttggctgttt ttcttttttg aaaatttcgt tatactctta ttcatggagg
     7981 aaaatgttat gaaaagaatc ttgacaattg gcggttctga tccatttgct ggtggaggaa
     8041 tccaaacaga tttaaaaact tttgagaatt ttggtctatt cggactaagc gccttgactt
     8101 ctataggggc actagatatt aatgagcgat ttatgctgga atctatatct ttggaattgc
     8161 ttaaacgaca gttagtttcg attgataaaa tgacttcatt agatggtatc aaaattgggt
     8221 tattgaattc aagtgaagca atcatggttg ttcgagactt cataaaaaca aaacaaaata
     8281 tcccaatcat tttagaccct gttttggcat tcaaagaaac tgaagctgtg atcaatcaat
     8341 tgtatatttc gcagctcatc gaagaacttt ttccacttgt tacgatgatt acgcctaatt
     8401 tggcagaagc tgctttactt tctggtcaag ctcttccctc ttcgttaaca gaaatgatcc
     8461 atctcgctaa agaactcact gcttcaggaa caaaaaacat tgtgatcaaa ggcggcacaa
     8521 ggatggctgg agatgaagct gtggatcttc tctattcaat tgatcattat gaggtatttt
     8581 ctcataaaaa aatcgacact acaacagtaa acggggccgg ttgtgccttt tcttctgcaa
     8641 taacagcgaa tcttgtgtta ggggcagatt ttaccaaagc agtagcagaa agtaaaaaat
     8701 ttgttgccca ttgtatttct aatggcgtgc tgatgaacga tcataccgga agtgtctggt
     8761 ctggcggtat tcaaaaagga ggaatgagaa atgaaaacta agcaattaac gatctatgct
     8821 atgctgattg cgttgacagt tgccctttca ttaacaattt tgatccctgt accggctacg
     8881 aatggatttg tgacattatg cgaggccggg atctataccg ctgcttttct ttatggtcca
     8941 acgggtggat tagttgttgg tgcttccagt ggtctattga tcgatttgat ttcaggatat
     9001 ccgcagtggg cagttttttc attagtgatc cacgggttac aaggatatgt tgctggttct
     9061 ttcagcaaat cttctactcg tatgtgggga ttagggcttt tattaggaag cttagttatg
     9121 atcatcggct atggtatcgc cggttggatt ttatatgatt ggccggctgg agtggcttct
     9181 ataccaggaa acatcttcca aaatctgatt ggaatgggac ttgcctttcc tataactgca
     9241 agtcttcaac gtattcaagc acggaagcaa tattaattga aaaagaaggt tttagaaaat
     9301 gaagttgtca gaaaaagaat taaaagacca attgactgaa atagttaatg acatattggc
     9361 tgaggcacac ttgaaaaaag gtgatctttt tgttttagga tgcagcacaa gcgaagtcgt
     9421 tggaggacat attggaaaaa attccagtgc ggaagttggt caatggatca tccgtacgtt
     9481 aaaagagttg ttggatccta aagaaattgc gctagctgtc caaggttgtg aacatttgaa
     9541 ccgagcatta gtagtagaac gaacagtggc agaagcaaaa aatttcgaaa ttgtctctgt
     9601 tgttcctgca ctacatgctg gaggtgcttg ttctgttgct gcttttgacc agttcaatga
     9661 tcctgtcgaa gtggaacacg tagttggaca agccggtatc gatatcggtg acacatcaat
     9721 cggtatgcat atcaagcatg tccaagtacc tgtcagacct cgtttgaaaa cccttggaca
     9781 agctcacgtg acagcccttc gctctcgtcc taaatatatc ggtggtccta gagcgaatta
     9841 ttgaaatgag taattgacag agcaatctga aaaacttcct cgcaaagtcc aactaatgaa
     9901 ctttaacgag gaagttttac ttgtttgttt tatataaccg agcttcataa ggtgctagta
     9961 tgcctttagc ttcaaattct ccgctagagg aatttgacaa gagtaatgtc caggactgat
    10021 ctgcttcttt tggaagttca tagtcagcaa gtgattctgt taagtttacc ataataagat
    10081 attgttcatc ttctaaatat ctttcataga cataaagttg tggatgttca tgaagatatt
    10141 ctttataaga accgtatatc agacctttat tggtttgtct aatgcggatc atgttcttat
    10201 agaattgcag gaccgactct ggttcatatt cttgttgttc tacattgatc gttttggtat
    10261 ttggattagt gatcagccaa ggttcatgtg tagaaaaacc agcatattgt tccgaagtcc
    10321 attgcatggg tgtcctgctg ttgtctctcg ttgtttcacg aatgatatcc aatgcttctt
    10381 ctcttgtctt accttccgca agcagccttt tataaagtct tttcgaatct actgcatcaa
    10441 cttgatcgat cgattcaaag ggcatattcg tcattccgat ttcctgtcct tgataaataa
    10501 atggcgtacc ttgcaaaaag aaatacatca tagcgattgc ttttgcagac atagcccaaa
    10561 aatcgggacg agtatctcca aacactgaaa cggcacgagg aacatcatgg ttttccatat
    10621 ataaagcgtt ccatccgata ccatccaatg aagtttgcca agcgcttaat gcatgtttca
    10681 atttaaggac atctaattgc ccttcttttt cttgctgcca gatatggata tgatcaaatt
    10741 cgaatatcat atcgaaataa ccgtctttcc ctacccattg tggtccttct tctgcagtga
    10801 caccacttgc ttctccgaca gtcatgatat cgtactcttc aaaaacatgt ttcaaatcgg
    10861 tcaaatgttc ttcgattcct gagacatttt gaaagggtga gaaagggttc tctgtggctt
    10921 ttactgaata ttcatctttt tttacatgag aaatcgcatc taaacgaaat ccatctatcc
    10981 ctttatccaa ccaccatcgg atcatattga atagttcttc tttcaatttc tcgctttccc
    11041 aatttaagtc tggttgttct tttgcaaaaa catgcagata atactgtcca gtttcatgag
    11101 aatactccca agcagaaccg ccgaaaatcg atgtccaatt attcggttct ttgccatcta
    11161 ccccgtcgac ccaaatgtaa taatcgcgat acgggctttc tttagatttt ttcgattctt
    11221 caaaccattg atgctgatcc gaactatggt tgatgacaag atccataatc actttgatcc
    11281 ctaattcatg tgcttctttt agcaataaat caaaatcttc catcgttccg aatctctctt
    11341 ctatatcttg atagtcactt acgtcataac cattatcgac aaatggagat tgatagatcg
    11401 gattgatcca gataaagccg ataccaagat ctttcaaata attaagtttt tcacggatcc
    11461 cattgatgtc tccttgacca tcgccattgc tatctttgaa actagcagga tagatttgat
    11521 agcctactgc ttctttccac catttttgtt cttgtatcat atcgtttcac tcctttaacg
    11581 gtccctcttg attataaccg attctatttt agaagtatcc ttgttaccgg taactagaaa
    11641 cgaaaagctc aacgaatctg ctaagtgcgt tcgttgagct ttattttatc atagatagcg
    11701 ggcaatcagt tgaatcattt gcttgggaaa ttcattcaag tcagtaatat ctacaaaacg
    11761 actttcaccg tagatcgact tgatttcttc tttgtcttgg ccaatagctg cggagacaaa
    11821 taggattcct tgacgttcat attctttcaa aacagactgg atatcttctt ttgctttttt
    11881 gcctgtataa tccggcaacg cttttggctg accgtcacta atattaagca acagtttggt
    11941 tgttgcggat tgtctgttta atttttctgc tgctaaacga agtgcagcac catcacgatt
    12001 gttttgcctt ggtttcatag tgactaattt ttcatttaac cattgaaatc cgtcttcaaa
    12061 ttctttataa gaaaacaaag aagtcttttc tcttgacgaa cggtctgctg tatctccgta
    12121 aatcaacaat gggatatcga ctttcttaga aaattcggca atggcaatcg ctgctttttt
    12181 tgccgcttcg atccgatcat cccgtatcat tgacccagat tcatcgatcc gtacagctac
    12241 cgctaaagaa ggttgctcat gtggcgggtt ctttttgtca aaatttctca aatcaccata
    12301 tgctactttg cttgcattga accgctgacc ttcgtatttt cctttttggt acgtctctgt
    12361 ttgttcatta tccagtaact ctaaaatttg ccggctaagt gattctacta ttggcattac
    12421 ttgcatgtgt agctcacgtg cttccggcgt ttttccttgg aaagctggtc ggtgaacgat
    12481 gagaccttct ttttcatgaa tcgtttcctt catgatttgt cgcgcttctt tattcagttg
    12541 cttcttgatt tccttatcat gtgcttccat gtcagaagca gacatttctt gagaatcatc
    12601 tcctgtttgt tgttcttttt gattttttcc ttcgatatcg ctactgcgtt ctttttgcat
    12661 ttgaggagga atctcgtttt tttctattgt ttcttcttgt ttctcttctc cctcatttgg
    12721 ttcttcagca gacaacgatt tcttttcatc taagcgcaga atttcttcag cattttcagt
    12781 gatgtttcgt tcatttgctt cttcggcgct aatggttctt tcttttgtat gctcagattg
    12841 aggatgaccc catttttctt ttctcaatat gtcctgaagt tccagtagca atccactgtc
    12901 agacaaagct tgttcaagaa ttgccaattc atctgaatca gtggataatt tatacaaaac
    12961 ttttggataa atcgactgga gtaaatcttg tccttgctta taggtatttg cccaataaaa
    13021 ataagaacgc attcctgcta cacctttgat ggcgttagct tttgcagttt catccaacaa
    13081 acggataatc tctgccatct ttaagagaag ttcttgtttt tggatcatag ttttcgccat
    13141 cgcacgttct gccatgactt ctaaggcagg aagatccatt ttttccgcat gttgcatccg
    13201 atctcttaaa gattcattta atgggcgatt tccttggtaa tttcgatttg tagtgatcac
    13261 aatgacgcaa tctggatggc gacgaatgat tcctgtcggt aaattcaaca tcccatttgg
    13321 ttcgagcgct gagttaagtg ccacaagaac agaggcatct cggatgacag tcggctcttg
    13381 aatctctaat aaatagcctt tttcaatcgc tcgaacgatt tcagaagggt aatagctgta
    13441 atggattgca tctggataat cagcttcgat cctttgaact agctctgcta atttttcttt
    13501 agcagaaaaa ggatcaatgc cgtaatggtt ctggatcagt gtcagtacag cttcaagtgt
    13561 atctgtttgg taaatagctt ctagtaattc ttggtcttgt tccttattag aatctactac
    13621 aggtaataag gctccgaaaa catctgattt gtccatatct gcaaaacagg tcacttttgt
    13681 atagggaagt tgcagatcag cagatagtgc tttggctaat tgtgtcttcc ccgacccggc
    13741 atctccttcc aaaagtacgt tactgatttt catttcagga tcttgccaat tccctttgat
    13801 ttcggaggcg atccgccatt cttctgcact tgtccgatgg cttttcggtt tcttccagat
    13861 catttgcttt tctagccgtg aaaaggttcg gttttctgtt agtgagaaat tctccatgct
    13921 tctcttcccc ttttgatcat ctctgtctat taatatccta gctcatctaa aagacgtttt
    13981 aaaatacgga atcgttcccc aatcagttct ccgtcttttt gtagtgtatc gtaatacaaa
    14041 atgatgtctt catcgatctt tggatttttg gtatctactt ctaccgtcat tcggttccct
    14101 tgtaatccaa tgatgtctac gactggattt tctggatcat aaaatttttc ataacgataa
    14161 gcatcttgga agtataaact tccttgcgca gctaagatcg ctaaatctaa aatacgtgtg
    14221 attggtaatt cttccgactg tcgtgaccat ttttctccag tatgtcgcca taccttgcct
    14281 gagatatcga cttttccacg atcattccac tgagcaaggc ccagtgaaag cccttttgca
    14341 tccatattta atgcatttct tccatcaact ttatcatagt cttctacaac gattactggt
    14401 ttatgtttta aatttgttgg tatttccatt attttaactc ctcatccttt tatttttagt
    14461 aaataagtaa attagtagtt tactcattta ctaaactata tgcgacatta tagaggattg
    14521 aagtgaggat tgcaaagaaa aagagcttca ttgatagaaa taaaaacacc atctcgttct
    14581 tttacacgct cattagtaac atgtagaaag tccaagatga tgttggaata ttttggctct
    14641 atactttttg atgttggtga acttcaccaa catctttttt gt
//
LOCUS       DANKEF010000058        14402 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000058 DANKEF010000000
VERSION     DANKEF010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14402
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.058"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            49..164
                     /gene="rrf"
                     /locus_tag="PWZ48_002166"
     rRNA            49..164
                     /gene="rrf"
                     /locus_tag="PWZ48_002166"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(217..687)
                     /locus_tag="PWZ48_002167"
     CDS             complement(217..687)
                     /locus_tag="PWZ48_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HDL2261814.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(846..1304)
                     /locus_tag="PWZ48_002168"
     CDS             complement(846..1304)
                     /locus_tag="PWZ48_002168"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDL2261815.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            complement(1475..2620)
                     /locus_tag="PWZ48_002169"
     CDS             complement(1475..2620)
                     /locus_tag="PWZ48_002169"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDL2261816.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            complement(2633..3550)
                     /locus_tag="PWZ48_002170"
     CDS             complement(2633..3550)
                     /locus_tag="PWZ48_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HDL2261817.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIYL"
     gene            3978..4541
                     /gene="efp"
                     /locus_tag="PWZ48_002171"
     CDS             3978..4541
                     /gene="efp"
                     /locus_tag="PWZ48_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDL2261818.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            4642..4812
                     /locus_tag="PWZ48_002172"
     CDS             4642..4812
                     /locus_tag="PWZ48_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261819.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            5027..6286
                     /gene="lysA"
                     /locus_tag="PWZ48_002173"
     CDS             5027..6286
                     /gene="lysA"
                     /locus_tag="PWZ48_002173"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDL2261820.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            6273..7073
                     /gene="dapF"
                     /locus_tag="PWZ48_002174"
     CDS             6273..7073
                     /gene="dapF"
                     /locus_tag="PWZ48_002174"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDL2261821.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            7076..7957
                     /locus_tag="PWZ48_002175"
     CDS             7076..7957
                     /locus_tag="PWZ48_002175"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDL2261822.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            7954..8670
                     /locus_tag="PWZ48_002176"
     CDS             7954..8670
                     /locus_tag="PWZ48_002176"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDL2261823.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            8676..9371
                     /gene="dapD"
                     /locus_tag="PWZ48_002177"
     CDS             8676..9371
                     /gene="dapD"
                     /locus_tag="PWZ48_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HDL2261824.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            9387..10388
                     /locus_tag="PWZ48_002178"
     CDS             9387..10388
                     /locus_tag="PWZ48_002178"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDL2261825.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            10385..11746
                     /locus_tag="PWZ48_002179"
     CDS             10385..11746
                     /locus_tag="PWZ48_002179"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HDL2261826.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            11706..12857
                     /locus_tag="PWZ48_002180"
     CDS             11706..12857
                     /locus_tag="PWZ48_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HDL2261827.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            12832..14001
                     /locus_tag="PWZ48_002181"
     CDS             12832..14001
                     /locus_tag="PWZ48_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2261828.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            14176..>14402
                     /locus_tag="PWZ48_002182"
     CDS             14176..>14402
                     /locus_tag="PWZ48_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2261829.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNV"
BASE COUNT         4657 a         2545 c         3071 g         4129 t
ORIGIN      
        1 tccagttttg agtgaacaaa atttactcaa taaaagataa caccacagtg tggtggcgat
       61 agcgagaagg atacacctgt aaccatgccg aacacagaag ttaagcttct tagcgccgat
      121 tgtagtgagg ggttgcccct tgtgagagta ggacgtcgcc acgcaaaggc aaaaagatga
      181 actagaaata gttcatcttt ttttattttt attaagttaa agaacggatt caattgcagc
      241 ggtcattttc aatggagaag tttgtggtgc aaatcgttcg attactttgc catttctatc
      301 aatcaaaaat ttagtaaaat tccattttat tgctttagtg acagtaccag gagcttcatt
      361 tttaagataa gtaaaaagag gatctgtatt ttctccattt acgtcaatca attgatgcat
      421 agggaaggta acgccataag tggttttaca agcttctgct gcttcagtcg ctgaatctag
      481 ctcttgttta aattgattgg aaggaaagcc taagataacc agaccttgtg tctgataagc
      541 ttggtaaagt tcttctaatt gtttaaattg aggtgctaga ccgcattttg ttgcagtgtt
      601 aacaatgatt atcggtttgc ctgcatattt tttcaatgaa tagacagtgc cgtcttctaa
      661 agtagtttca aaattataaa tagacataaa gttttctcct ttctatttta tataataaaa
      721 agagcgctaa actgaaaact agctatttgt tttcccgttt agaactcttt tcagacaata
      781 aatatttaat aaaatacgaa gataagatag ctatattgct tctcatcttt acgttctttt
      841 agaaactatg cttctacttc agaccattca tcgcgttttg ctaaaaattc acgagccaaa
      901 gcttgtgcac cttctagtgt atggctggca gcccagccac attgtgtttc attgcttgca
      961 ggtacttcag tagcttcaag aacatctttc attgttgctt caagaacatc taaaatgtct
     1021 tcgtagcttt catgattcaa tacagtcaag taaaagcctg tttggcatcc cattggagac
     1081 cagtctacga tgtaatctgc gtgattgcgg attagttcgg cagtcaagtg ctcaagtgaa
     1141 tgtagacttg gcatgtccat gtgttcttta tttggttgtt taaagcggac gtcgtattta
     1201 atgatgacat cgccatgcgc acccgttttt cgatcagcta cacgtacata aggtgctttg
     1261 acttttgtat ggtcaagatt aaaactttcg acattcattc tcatttgtaa gactcctatc
     1321 tgtttgttca ttcgttttga tgcttttcac taaagaatta tttagtgaaa tttacaaaaa
     1381 cgtctttcta cttgattcta acacttatcg tttccaaggt gtagcaatct gaaatatttt
     1441 ttagattttt ctgctaagtc gtttattggg acttctagtt cttgattctg tcaaatgcct
     1501 gagctaaatc gtttatgaga tcctgaccat actcaattcc tacagaaaga cggatcaagc
     1561 cgtcctttat gccagcctct tctcgttttt cttttggaat agacgcatga gtcatgacag
     1621 aaggaatttc cactaaactt tctacgccac ctaaactttc tgctaaagta aatagttgga
     1681 gtgattcaac aaatggaata gcagattctt cattttttaa agtaaaagaa atcattccgc
     1741 taaagccgct catctgctgc ttagcaactc catggagttc atgatcgggt aatcctggat
     1801 agtagacctt ttctactgct ggatgagaaa atagaaaatc agctaccaca aaagcatttt
     1861 tttgatgttc ctccatacga acacttagtg tcttgattcc gcgctgaagt aaccagctat
     1921 cttggggacc aagtacggct ccgatagcat tttggtaaaa tccgatctga tcggctagat
     1981 ctttatggtt agaagtcact aaaccagcta ctacatcgct gtgtccgcct agatatttgg
     2041 taccgctatg gacaacgata tcagaaccga gatctaaagg tctttgaaaa taaggagtag
     2101 caaatgtatt gtcagcaata actatcaatc catgttgatg agccagagtg gcactttttt
     2161 ctaaatcagt aatctttaga agcggattgc taggtgtttc aagataaagt gcttttgtat
     2221 tgggtttgat ggattgttct attttatcaa gattactcgt atctatgatc gtgtactcga
     2281 gaccattttt agtaagaact ttgtcaaata gacggaatgt tcctccatag acatcatctc
     2341 ctaataagat atgatcgcca gcttgaaaaa gagaaaaaac ggcatgaatc ccagaaagac
     2401 cagaagaaaa agcgaatcct cgaacaccgc cttccaaatc agcaatcagt tcttccaatg
     2461 caaaacgagt aggattacca gatcttgaat attcatattg tttaggctgg ccgacgccat
     2521 cttgccgata agtggatgtt tgataaatag gtacactgac agctcctgtt gtgggatctt
     2581 cactgatgcc tccgtggatc aattttgttt gaatgtgcat gtttctttcc tcctataagt
     2641 aaatgttttt tgataaatag cggtctgctg cgtctggaaa aatcgtaacg acacgatgac
     2701 caggtggtaa acgacgaatt tctcttaatg ctgctgcaaa agccgcacca ctcgaacttc
     2761 caactaatag tccttgttct cgtgctaact gcctagtata gttaaaccct tctacatcag
     2821 agattgtctc gatctgattg attgaaaggg gagaaagaaa cggcggaatg aattctacac
     2881 cgataccctc aatttcgtga ggggctggat caccaccatt taacacagag ccctctggtt
     2941 ctacaccgat tattcgaatg tcgggatatt tttctttcaa aaaggtagaa gttccagcaa
     3001 aggttccacc actcccaatt cctgctacaa aactatggat attttctttg atttcatgaa
     3061 agatttctgg ccctaacgta tgatagtaag cagcaggatt atctttattt tcaaattgaa
     3121 gaggcagata gctgtttgat atctcctctg caagtttttt acttttgctg atggcaccga
     3181 tgattccttg ctcactagga gaatggatga tctttgctcc caatgctttc atcaattgtt
     3241 gtttttctaa gctaaacttc tctggaacaa caaaaaccgt tcttaatttg tatgtgagtg
     3301 cggccagagc gatcccaatt cctgtatttc cggcagttgg ttcgatgatc gtcgtttttt
     3361 cattgatttt tccttgggca aatccttctt tgatcaaata actgcccaat ctatctttga
     3421 tacttcctcc aggatttaaa tattctagtt ttgcataaat agcagaacct ttaggcatag
     3481 aatagtgctg attgtcaaag cgataaagcg gtgtggaacc aatagcttct gtaattgtcg
     3541 agataatcat gtgggcctcc taaaaaaaga taataaaaaa aggagctgac tattaaatca
     3601 gctccttgaa agacatagag aaaaattgag tagataaaaa gataagttac ctggtactca
     3661 ttctatgaag ggcaattaat agacatgaca aatagtatgc tgcttttatg caccatacaa
     3721 cagcaacgat tcaattgttt ttggttgctg actgtcatgt gtatccctcc aagtaaaatt
     3781 tttattaacg atagcatgaa aaaaaataaa agtcaaaggc ttgaaagaaa aaaatatttc
     3841 gatagcatcg tttacccgtg tttatttttc tgacaaatag gcatgagacc ttaaaagata
     3901 gagaatactc tagttttctg gccggttgtc tgatacaata aagttgatta tgataaagaa
     3961 aagaaggagc aaaaataatg atctcagcaa gtgatttaag agccggtatg actttcgtac
     4021 aagacggcaa actgattaaa gttctggatg caagccacca taaacctggt aaaggaaata
     4081 cagttatgcg tatgaaactg aaagatgtcc gttcaggtgc aacatacgac acaacgttca
     4141 gaccagaaga aaaatttgaa aaagcacata tcgacacaaa acctgtacaa tatttgtaca
     4201 cgatggatga tacagctttc ttcatggatt tggaaacata tgagcaatat gaaattcctg
     4261 tagaaacagt agaagaagaa atgaaatata tcctagaaaa catggaagta aaaatccaat
     4321 ttttcggtag cgaagtcatc ggcgttcaat taccaacaac tgtcgtatta cgtgttgtgg
     4381 aaacacaacc ttccatcaaa ggtgcaacag tcactggttc aggtaagcca gcgacaatgg
     4441 aaactggctt agtagtcaat gttcctgact tcatcgaaac agatgaattg ttggaaatca
     4501 atacagcaga aggttcttat gtaaaacgtg cgtcaaaata attcgctaac ggattgataa
     4561 gaatttgaaa acagagcttg acctcttgaa aggcagatca tcggatctgc ctttcaagag
     4621 gttttttgtg gaatcagcaa attgaaaaat aaaaaagtat ttgattttat ttatattttg
     4681 aaagatgaat ggcaaaagct agagaaagaa aaaactgcac ttatttatga agaaattgaa
     4741 atgattcata aaattgaaaa tatccgaact attccgaact atattgaagc gaaaatattt
     4801 tttcatcact aaatagtgtt gaatttatgt ttttatgtat aaatagcttc taatagttcg
     4861 ggtattttgt ttttataacc atttttattc ttatttttaa aaaaatattt cgaaatatac
     4921 tagacaattc agaaaatttt gtttaatatt attccaataa aattcaaatc tttatcaaat
     4981 gaaattccaa taaaaacgga aaaaaagtga aaagaggaat gaaaaaatga cagaattgat
     5041 aattggcgga gtcacagcaa gcacattggc agaaaagtat aagacacctc tgtatgtgta
     5101 tgatgaagaa aagatgagac aaacgatgct gtcattcaag cgaggctttg tatcgaaacg
     5161 ttttgataca aaagtgcttt acgcctctaa agcattccaa acgattgaga tgctgcattt
     5221 agttcgtgaa tatggattcg ggctagatgt tgtcagtggt ggggaaatct ataccgcttt
     5281 gcaagctgat tttcctagag atactatcta cttccatggt aacaacaaga cgccagagga
     5341 actgatctat gcacttgaaa atgatttgtt gcatattgtt gccgataatt tgatggaagt
     5401 tgaattatta gcagaattaa gccaacgcta tttaaaaaaa atctgtatca tgcttcggtt
     5461 gaatgtcgga gtagaagcac atactcatga atatatcgtc actgcgcata ttgattcaaa
     5521 attcggcatg agctatgaaa gtaaagagtg ccaacaagcc cttcagttga taaaagaaag
     5581 cagctatcta gagttagaag gatttcatgc tcatatcggg tcacaaattt ttgatgttac
     5641 tgcttggtta gctgaaatcg acaaattgac gaattactta accgattttg accaacctct
     5701 ttccttgaat cttggcggag ggtttgggat tcgttataca caaaatgatc agccgatccc
     5761 agtcgaagag gcactaaaaa aactgattga atatacggaa gaagctttgg aaaaacgaaa
     5821 actgaccatt cgtcaattac tgatcgaacc gggtagaagt attgttggag aagctggtac
     5881 aacactatac acgattggtt tcatcaaaaa gacaccacat aaaaaatatt actttgtaga
     5941 tggcgggatg acagataata tccgtccagc tctgtatcag gcagaatacg attgtgattt
     6001 agcagcaaaa ctgtcggaag aaaaaacaga gaaagtgacg attgcaggaa aaatgtgtga
     6061 atcaggcgat gtggtaatta aagaagctta tttgccgaaa gcagaatcgg gtgatctttt
     6121 agctgtttat tccacgggag cttatggata ctcgatgagc agcaactaca atcgagcgac
     6181 acgaccagca gttgtttttg tcaataatgg ccaatcaaga ctgattgttc gcagacaagc
     6241 attttctgat ctactaaggg gggaagtttc ctatgatcct tcataaatat aatggctgcg
     6301 gaaatgattt tatcttatta gattacgcag aagacacaga ttatagtcaa ttagccgctt
     6361 ctttatgcac aaaagaaaaa tatgatatag acggattgat cgccgtaaaa atcgatccgt
     6421 tggaaatgat ctattacaac aaagatggca gccgcgcgcc gatgtgcgga aatggcattc
     6481 gctgttttgc tcgatacgta aatgatcaga acatgatgaa tgaacaggaa ttcaacgtag
     6541 agacattagc aggcacaaga aaaattacgg tgttggatcc ctcacctttt tattgcagcg
     6601 tagatatggg agtgccagat gattctccgg aaaaaatcaa agctaatcaa acttatccaa
     6661 taaaaaacca ggaaatagag atcaatggtg aaaaagtaac gatcactagt ttatttttag
     6721 gaacgattca taccgttgtt tttgttgaaa atgcgcgtga ggaagtctgg agaaaaagag
     6781 gtgaactgat ctgtcatcat cctttgttca aagaaaaaac caatgtcaac tttgtagaag
     6841 tagtcaatga acaagaattg atcgtccgta cctatgaaag aggagtaggt tggacacttg
     6901 cttgcggtac aggttgctgt gcctcttatg tggttgctaa ggaaactggg aaaatttcag
     6961 cagatcaggt agttgttcac ttggaacaag gagacttgat gatctctggt gaggatgcga
     7021 tacaaatggc tggcccagca gttcatgaat ggacaagaat attggaggaa tgaagatgat
     7081 aaaaggttcg atcgtggcgc tgatcacgcc gatgaatgaa gagggatcag tagattatgc
     7141 tggtttggaa aaattgatcc aattccattt agatgaacaa acagatggat tattagtttt
     7201 aggtacaaca ggagaatctt ctacgctcac acaatctgag gaggagcaga tccttcaact
     7261 gacagtaaag aaagtggctg gccgtgtgcc ggtgattgca ggagctggaa cgaataatac
     7321 gaaagaaacg attgaaaaag cgaagcattt tgcttctttg ggggcagacg cacttttggt
     7381 catcacccct tactacaaca aaacaagtga tgcaggacta gctgctcatt ttacagcaat
     7441 tgcagaagcc tcaccgattc cgcttatttt gtataatgtt ccttctagaa caggcatgtc
     7501 gattccaatc catgtattag taaatttagc agaacatcct aatatcattg gtttgaaaga
     7561 agcctcagga gatatggctt atgtgatgga tgccgctcgg ttgattggag aagaattctt
     7621 cctttacagt ggaaatgatg atttgatcct accagtaatg agtgtaggag gaagcggtgt
     7681 aataagtgta tgggcgaata tccaacctaa aatagtccat gaattagtaa aagatacgca
     7741 ggacggaaga tggcagcaag caaaagaaaa gcaattaaat gcacttgaac tgatccatgc
     7801 cttgttcagt gagaccaatc ctattcctgt caaagcagcc atgtcattat tagacttacc
     7861 ttccggacct ctacgcctgc ctttagtttc tctttcaaaa gagaaaaaga aacaattagc
     7921 tcagcttttg ttaaaagaaa ggacggttga aaaatgaaaa tcggactgat tggcagtgga
     7981 cagatgggaa atagagtcga agaagttgca caagaaagag gagatacggt ccttctttta
     8041 cggacggcac ctaaagaagc aactacgctt gcttttacgg aactgccaga actgcttatt
     8101 gatttttctc atccagataa tttagagaaa atcctttcgt atagcttatc atatcaactt
     8161 ccgttagtca ttggcacgac aggttataca caatcacagt tccaagaaat aaaagaacat
     8221 gccaaacatg tacctgtgat gtatagtgct aatttttcgt tcggcatcat ggtgatgaat
     8281 caactaatca agcaagcagc tgcaatgttg caaggctggc agatcgaact gattgaaaaa
     8341 catcatagtc gtaaaaaaga tgcaccatct ggtactgcaa atatgctgct tcaaacaatc
     8401 caagaagtgc aaaagctgca gcccgtatac aactggcaag ggaaaaaaag agaagaaaac
     8461 caaattggtg tacacagtat tcgtgcaggt tctttacctg gtgaacatga tgtattgttt
     8521 gctgctactg atgaaatatt tacgatcaaa catgaatctt tttctaatcg aatctttgct
     8581 gcaggcgcag tagaagcagc ggactggttg aaagatcagt caccaggtta ttacaaatta
     8641 gaagatttat taatggacaa aggggtgtaa aaaacgtgga agctcaagaa atcatccgct
     8701 acattcaaac ggcatctaaa aagacgcctg tgaaagtcta tctgaatgta accgagccaa
     8761 tcgaatttaa agaaagtaaa gtttttggtg aaggaagcag cttcgttgtt tttggtgact
     8821 atgagacgat tgctccggtt ttagaagctg aatcagaaaa aatcattgaa atcgaaatcg
     8881 aaactgctgc aagatacagc gcagttcctt tgctagatat taaaaaaatt aatgctcgta
     8941 tcgaaccagg tgctattatc agagatcaag tgtcaatcgg caacaatgcc gtgatcatga
     9001 tgggagcgat tatcaatatt ggtgcagtaa tcggtgaaaa cacgatgatt gatatgggtg
     9061 ctgtcttagg tggacgagcg acagtgggca aaaactgcca tattggtgca ggagctgtct
     9121 tagcaggtgt gatcgaacca gcatcggcca aaccggttat cgtagaagac ggcgtactgg
     9181 taggtgcgaa tgcagtgatt gttgaaggtg tccatatcgg aaaagatgca gtcgtggcag
     9241 caggagcagt ggttttagaa gatgtggcag cagaaactgt tgtcggtgga atacctgcac
     9301 gtgtattaaa aataagtgat gatcagacaa aagaaaatac agcattgatt gctgcgttac
     9361 gcgaattata gaggagatgg agaaaaatgc gcgaaataga tacctgtatt gtgggggcaa
     9421 ccggattagt cggccaaacg atgctcaaag tgttagaaga atatgatttt ccagtaggac
     9481 gattgaaatt attagcttca aaacgttcag ccggtaaagt ttgtgtattc aagaaaaaga
     9541 aacacactgt acaggaattg acagaaactt catttgaagg atatgattta gcgctatttt
     9601 ctgcaggtgc ttctgtatcc aaagagtttg cgccaatcgc acgaagtcag ggactatttg
     9661 tcatagataa ttcctcagca tggcgggaag attcggatat tccattaatc gttccagagg
     9721 tcaatctgga agactataca ttgaatcggc tgattgcaaa tccaaattgt tcaaccattc
     9781 aagcggtact tccgcttaaa gctttacaag aagcctttgg tatccagcga gtcaattatt
     9841 cgacctatca agcagtttct ggttccgggc aaaaaggcat tgaagatctt catcgtacgc
     9901 aaaatgacga ggaaccagtt ttgtatcctt ataatattag ccaaacggtg attccggaaa
     9961 tcgatcagcc tttggaaaat gggtatacaa aagaagaaca gaaaatgata aatgagaccc
    10021 gtaagattct gcatcagcct gctttgccag tatcagcaac ctgcgtacgt gtgccaatcg
    10081 aaaatggaca tggagtctct gtggcagttc aactgcaaaa agagtttacg acggaagaaa
    10141 ttagagagtg tctcagccag tttccaggga ttcaattaga agatgatcta gcagcacata
    10201 aatatccgac gagcattctt gctagaggaa cagatatggt ttatgttggg aggatcagaa
    10261 aagacacttc tttagagaac ggattgttgt tctatacgac tgccgataac atacgcaaag
    10321 gagccgctgc gaatgccgta cagattgctg cagctttaat gcagaaaata gcggaggtgg
    10381 caagatgagt aatacgctga aagtagcgaa attcggcgga acatcgatgt ctcatgaaga
    10441 gcaattcaaa aaagtccgta caattgttct ttcagatccc aatcgccaag tgatcgttgt
    10501 ttctgcttta ggcaaacgta cgtcagatga tgacaaagta acagacttac tttatttgat
    10561 ttatgcttac cttcagcatc atgttgattg gacacccctt tggaaaagga tttgtgatcg
    10621 tttcattcaa gtaagagatg ttcttgccct gcagttacct attgaagatg aactagcacg
    10681 catacagaaa caactggaag acgaaaagat aactgaagat tatttagtaa gtagaggtga
    10741 atatctgact gctcaactga tggctgagta tcttggttac cagtttattg acgcaaaaga
    10801 cctgattttc tttgactatg atgggaaaat cgatcaggaa aaaacagcac aggaaatcac
    10861 aaaagcattt aaaagatatc cgaaaatggt tgttccaggg ttttacgggg cgaaccctgc
    10921 aggaaaagaa aaattattgg gccgtggggg aagcgacatt accggcgcga tactggcgaa
    10981 gatgttgcaa gcggaaagtt atgagaattg gacggatgtt tcaggaatca tgatggctga
    11041 tccgcgaatc atcgatcatc ctaaaatcat cgaggaaatc tcttttagag aactgagaga
    11101 gatggcttat atgggggcta acgttcttca tgaagcagct gttttcccag ttcaagaggc
    11161 tgatatcccc atccaaataa aaaataccaa tgatcctttc gctcaaggga caaaaatatc
    11221 gaatcacgaa atagaaaagg aaaatggact gacaggaata gctggacgca aggattttct
    11281 ttccatcact ttattcaaac gtcatatgtc cgatgaaatc gggtttatct ggaaagcaat
    11341 gagcattttt gctaaacatg agatatcaat cgaacatatt ccttcaggga tcgacaatat
    11401 cggagtgatc gtctcagcag aagcgattgc cgataagtta tttctcatca cgaaagaatt
    11461 aaaagaaaca cttggcgtgg aagaaatcgt cgtgatcgaa gacttagctt tgatctctgt
    11521 cgttgggggt ccgcataaag aattgattgg attatctggg aaagtgttat cgatattgaa
    11581 caaattagaa attcgcacat cgatcttatc acaaggagca caggagttga atctgatcat
    11641 cggtgtaccg aataatcaat atgaaacagt agtgaagggt atctatgaag ggatggtgaa
    11701 tacggatgct gaaagaatat cgaatggcac tccatcaaat tcctgaaatc gggtttcatg
    11761 aattcaagac aaaaaaatat ttatatgaac aagtaaaaaa cagcgaggcc gttatccatg
    11821 aaatcggcga aacaggacta ctcctttatt ttgataatca gcaagaagaa acgattgctt
    11881 ttcgaacaga tatcgatgca ctgccagtca aagaagaaac gaatctctct tttgcttcta
    11941 agcatgaagg ttatatgcat gcatgtggtc atgacggaca tatggcgatg cttttagggt
    12001 tgactgactt tataactaaa cacattgagg atcttattta taatgtagta ctgatctatc
    12061 agccatcaga agaaatttcc ggtggcgcac aaagcgttat tcagtcagga ttgttggata
    12121 aatatgaagt acaagcaata tttggctttc atctatggcc tggactagct aagggagaag
    12181 ttttctctcg accaggacct ttgatggcac aaagcagtga aacagacatt atcattaaag
    12241 gccagtctgc acatattgct tctagtgaaa aaggaatcga tagtctggag gcagctgtac
    12301 gatttttgaa gaatgtttat gattttgacc ggcaattacc ggcagagtgg aatcatctaa
    12361 tgaaatttgg ccaaatccaa ggtggaacga tccgaaatgt tttagctgga gaagttattg
    12421 tatccggaag tatccgttct tacagttcag atatgcaaaa ttatttgaag aggggattag
    12481 accatttagc agaacagttt cactctacag ctactatttc ttttcgctat aatgacggct
    12541 atccagctgt aatcaaccat agaggcttat atgcagcact gttagattca aacaaactac
    12601 atgaattaga tgctccagtt cttcaagccg aagattttgg catatatacg caacaatatc
    12661 catgtgtatt tttcttttta ggtgtcggtg atacccctgc tttacatgat ccgacgttcg
    12721 attttgatat ggatgtttta gaaaatggtg tagaatggta caaaacgatt ctttttacag
    12781 aaaatcttct tgtaaaaatc gatagcagtt caaaagagga gcgagtaaat aatgatgaat
    12841 ttatccaatc gatttagtcc taggctttct agaatagaag tatcaaaaat acgacaattc
    12901 gatcagcaaa tatcgtcgat accagatgtg atcaaactca ccctgggaga accggatttt
    12961 ccaactcctg aacatgtcaa gcaagcagga atagcagcga ttgaagaaga cttttctcac
    13021 tataccggaa tgagaggatt agaggaactg cgggaagcgg cttgtatatt tcagcaacag
    13081 cgttatggat taacttatga tccgcaaacg gaagttttga cgacagttgg tgcaacggaa
    13141 gcgattgcct ctgcgttgtt atctgttttg gaagaaggag acaaggttct tattccagcg
    13201 cctgcttatt caggatatca gccattagtg gaactagctg gtgctgaatt gatcccgata
    13261 gatacttctg acacagggtt tgtctgtcag ccggaacaat ttgaacgagc atttgaacaa
    13321 tatggatcag ctgtcaaagc agtcatttta aactatccta acaatcctac tgggacgaca
    13381 ttgtctgcaa aacaattgaa agcaatcgct gaggttttaa aaaaatatcc ggtatttgtt
    13441 ataagtgatg aagtatatgc tgaattgact tatagtggaa cgcatatgtc catagctact
    13501 tatcttccag aacaaacaat cgtgataagc gggttatcca agtcacatgc gatgactggt
    13561 tggcgagtgg gctttatttt tgctcaaaaa gcccttattg acgaactaat taaagtccat
    13621 caatatctgg tgactgccgc tacaacgatg tcgcaaaaag ctgcggctga agcgctaata
    13681 aatggggtga aagatagcga gaatatgaaa gagcaatatc aaacacgaag agactatctg
    13741 gtccagcaat tggcgccttt aggatttgaa gtgacccagc caaacggtgc attttacttg
    13801 ttttgcaaac tcccagctgc cattcaaact gactcttggc agttttgtgt agaattagcg
    13861 gaacaggcaa aagttgcgtg tatcccagga attgcatttg gcccagaagg agaaggttat
    13921 atccggattt cttatgctag cagcatggaa aaattacatg aggcctgcaa acggatcaaa
    13981 gactttttaa cagaacagtg attcatagat tttatcaaaa aaacaatcgt taattttgcg
    14041 gtatattctg caaggagacg attgtttttt aaatttagtt caaatgaacg cgtttctttt
    14101 gattcataac taatgtaatg ttaagtttaa attgtttaga atgattttaa aagaatattc
    14161 aaggaggttt catcaatgga aaaaatcaaa gttgtcattg taggtgcatc tcatggaggg
    14221 catcaatcga tcttggaact attgacacga tacggtgaaa atgtagatat cacgctattt
    14281 gaagcaggag actatatttc cttcatgtca tgtggtatgg agctgtattt agaagacaag
    14341 gtaacagatg taaatgatgt acgtaatttc agaccagaaa actttccgca atccaatgtt
    14401 ca
//
LOCUS       DANKEF010000059        14119 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000059 DANKEF010000000
VERSION     DANKEF010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14119
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.059"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..556)
                     /locus_tag="PWZ48_002183"
     CDS             complement(<1..556)
                     /locus_tag="PWZ48_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL2261830.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(572..1051)
                     /locus_tag="PWZ48_002184"
     CDS             complement(572..1051)
                     /locus_tag="PWZ48_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDL2261831.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1057..2081)
                     /locus_tag="PWZ48_002185"
                     /pseudo
     CDS             complement(1057..2081)
                     /locus_tag="PWZ48_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2098..3204)
                     /locus_tag="PWZ48_002186"
     CDS             complement(2098..3204)
                     /locus_tag="PWZ48_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HDL2261832.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="PWZ48_002187"
                     /gene_synonym="adhC"
     CDS             complement(3433..6030)
                     /gene="adhE"
                     /locus_tag="PWZ48_002187"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDL2261833.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6336..6716)
                     /gene="yajC"
                     /locus_tag="PWZ48_002188"
     CDS             complement(6336..6716)
                     /gene="yajC"
                     /locus_tag="PWZ48_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDL2261834.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6813..7958)
                     /gene="tgt"
                     /locus_tag="PWZ48_002189"
     CDS             complement(6813..7958)
                     /gene="tgt"
                     /locus_tag="PWZ48_002189"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDL2261835.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8176..8874
                     /locus_tag="PWZ48_002190"
     CDS             8176..8874
                     /locus_tag="PWZ48_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HDL2261836.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9042..11681)
                     /locus_tag="PWZ48_002191"
     CDS             complement(9042..11681)
                     /locus_tag="PWZ48_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HDL2261837.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12300..13331)
                     /gene="queA"
                     /locus_tag="PWZ48_002192"
     CDS             complement(12300..13331)
                     /gene="queA"
                     /locus_tag="PWZ48_002192"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDL2261838.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13524..>14119
                     /locus_tag="PWZ48_002193"
     CDS             13524..>14119
                     /locus_tag="PWZ48_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDL2261839.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEA"
BASE COUNT         3959 a         3096 c         2543 g         4521 t
ORIGIN      
        1 aagcgcctac gagataacct cccataaaca aagcagagat cgaagcacca ccagcaacaa
       61 gtgaatacat gataaaagca gtacttggag gaatgaccat acctgtcgga gctgacgcga
      121 tattgaccgc tgtcgcaaac ttccggtcat atccttcttc cacttgctga gggatcaaca
      181 ctccaccaat cgcggtagaa gcggcaatgg ccgagctaga gatggaacca aataaagcat
      241 tccctaacac attcgtatga gccaaagccc ccggcacgcg cccgacgaac aacatcgcaa
      301 aatccactag tttcttagct atccctccat tattcatgat aatgcccgag agaacgaaaa
      361 acggaatagc gatcagcgaa aaactattga tacttcccac cattttttga gctgaactaa
      421 aagcagaaac atcaaacggt ataactaaca ataaagtcat catcgatgag aatgcaatac
      481 ttatagcaat cggcatccct aatacaagca acacaacaaa tacaaaaaat aacactaatc
      541 cggcttctat tgccatttta ttcctccatt tttatatttt ttcttgaatt gctggcttgt
      601 tgcttaatgt atcgattaga ttgatcaggc tatagataat gatgagtacc cccgatacag
      661 gtactgacag ataaacatat cccataggaa tacttaaaga aggcgagatc tgccccattg
      721 tcaatgtgac agcattccat ccgccataca ccatgatcat cgcagcaaat accagaaata
      781 aactttgaac aacaacttct aaaattttac tttttttgcc agttaatttt tcacttaaaa
      841 ccgttacggc taaacgtgct ttttgtccca caacatacgc ggaagacaac atggcaaacc
      901 agacaagacc gaatcgaaca agctcttccg tgaccgtatt tggatttcct aaaaccgttc
      961 tggaaaatac ttgccataaa acaacagcga ccatcccaac aagcaaaaca gctcccagga
     1021 tctctaatac cttgtttaaa atcattttca ttacagctac gctccttgct cttggatctt
     1081 ttgataaagg tctttgtagg taggatcgtt tttgaatata tcatgaagtg gggcaactgc
     1141 ctctcgaaac ggtgtattat ctacatcatt gaattggact ccatgttctt cttgtgctaa
     1201 agctttttct tcttcaattg cttttgcaaa agcttcaatt tcaaaaaccg ttgattcttt
     1261 cgctgcttct tcaatggctt tttcctgttc atcagttagc tgttctttga tggcatcatt
     1321 catgatgatg atatctggca cacgtgtatg ctcatcatag gaatagtatt tagctacccc
     1381 gccatgtcca gctgtataca agacaaattc attgttttct gatccgttga tcaaattgga
     1441 ttgaagagac gtgtacactt catcacttcc cataggtacg ggcgaaccgc ctaaaagctc
     1501 taccattttg atagcggttt cgctttgcat cacacggatc ttctttccct tcaaatcttc
     1561 cggcgtatgg atcggaccgt ctaccatata aaagctgcgc tgaccagaat cataataggt
     1621 caatccggta aatcctaaat ttttggtcga ttggtagatt ggctccatta tttcttcatt
     1681 atccatcacc ttaaagaaat gttcttcatc attgaataga taaggaacgg aaaaaatcga
     1741 ataatcttta gagaaacttt ccaacgctga ggcacttact tttgtgaaat ctactgctcc
     1801 tgactgtgtc aattcgatca gttcacgttc tccacccagt tgtccatctg ggaaatactg
     1861 gatctgtacg gaaccatctg ttttttcttt tactaaacgc ccaaattcat tcattgcatc
     1921 aattactggc tgttgcttgc ataagcaaag cgcaaggtaa ctatctcatt tacgtctgca
     1981 tctgcgacag ttgccacact agaattattt ccgcagccgc ttagacctag cagacctagg
     2041 ctaagcgaac ttgctgcaaa cagcagtgat gtttttttca tatgggtttc ctttctttta
     2101 acaataagag acgagcaatt ctgtcaaaca aagcaccgtt aacgactgtc cataaggcat
     2161 tgctgtgatt ccgatattcc gataaaaatc taaactgtcc cccattcctg tcccgattga
     2221 aacgttttga acttctcctt tttcatcgat ttgttcaagc aatccttgga tagcacgata
     2281 agcgacttct ttatattctt gtggtaaata acgtttatgg accgctttca aaataccgta
     2341 agcaaatcct gcagttgcag aagattctac gtaagaagaa ggatcatcca acaacgtatg
     2401 ccataaaccg ctttcagact gataagtttt taatgcacgc acttgtgctt caagtgtttc
     2461 gatcaataat ttgcgcaatg catcttcttt cgataattct aagatttcaa tgatctctgg
     2521 aatagcaatc gtcaaccagc agtttccacg agcccaaaat gcttcggcat agttatgatt
     2581 tccttcaaac gtccagccgt gataccacaa tcctgttcgt ttatccatca agtatttgat
     2641 atggatcata aactggtaac gagcttcttc taaatattct gggcgattca acagtttccc
     2701 gatttttgcc aaaggaagca ctgtcatcat caatgtatcg tcccacaact gattcttatt
     2761 ttccggtcca tatgtggcat gctgtaaccc gtcttcattt gtccgaggca tgtcattcat
     2821 gacccattct gcccattgtt ctaaataagg caaatagcgt gaatctttcg tgtcttcata
     2881 caagtaagcc atagtcaata gcggtgccat tgtattgacg tttttgggcg gagcgccttc
     2941 ttccatacga tcctcaaacc attcattgac cacttgataa gcgcgaggat tttttgtcat
     3001 ctggtaattt ttataaatac catatagacc gacaccttgt ggccaattcc acactgtcca
     3061 gcttttgtca tctactttca gtccatcaaa gtccaacaaa aattcaccat tatcgtcttt
     3121 gatcgttgtc aggttttcaa cgagcaggtc aattttttct ttcactaatt ctttaggtac
     3181 aaaaatctct tctttaataa tcatctttac gccacctttt taaacgcttt cattttatat
     3241 ccttaatata gcagtaatac cgtccactat aaatatcgct ttttgaacaa aaactgtcta
     3301 aaattgacct aaatgttaaa taacaaaagt aactttcagt tacttggaaa ttaaaaaaag
     3361 aacttggaga aacaacgttt tccaagttct ttttcatcta ttttcttatc ttttttctac
     3421 gctttctttt gcttatccgt tgtaagcatc aatgatgatc tgtttcaatt cagagatcaa
     3481 tggttctttc ggattagctg tcgtacattg gtcttcgtaa gctaattcag ccatacggtc
     3541 tacggttgta tctagtgttt cttgtgtgac tccttgagct tttagactca tgttgatgcc
     3601 gacagatttt cctaattcat aaacagccgt tgctaaagct tcgactaatt cctctgttgt
     3661 ttcgcctttc agacctaaga attttgcgat atctgcgtaa tctgtatctg cacggaaata
     3721 gtcatattta gggaacatcg catgtttttg tggatctttt gcattatagc ggataatatg
     3781 cggtaaaaga atcgcatttg tccgtccatg agggatcccg tattccccgc cgattttatg
     3841 tgcgactgag tgacaaatac ctaggaatgc gttagcaaac gccataccag ccattgtaga
     3901 agcgttgtgc attttttcac gtgattccat atcaggtgtt tttactgatt tttctaaata
     3961 gtcaaagact aacttgattg cttgtaggct caatccacgt gtataatctg aagccatgac
     4021 tgaaacatag gattcgattg catgtgttaa cacatccatc ccggtatctg ctgtgacaga
     4081 tgctggaact gacatgacga attgaggatc gacgattgcg acatctggcg tcaatgcata
     4141 atctgctaac ggatatttca catgtgtttc actgtcagtg atgaccgcaa atggcgttac
     4201 ttcagaacca gtccctgatg ttgtcgggat acatacgaat tgtgtttttt ctggtttgtc
     4261 gatcttatat gtccgtttac ggatatccaa gaatttttgt tttgcaccaa agaaagatgt
     4321 gtctgggtgt tcatagaaca tccacattcc cttagctgcg tccattgcag aaccgcctcc
     4381 aagagcgatt actgtatctg gcttgaagtc tgccatcagt ttcgttcctg cgtatacggt
     4441 atttgtcgat gggttcggtt caacatctga gaagacttcg atcttcacat catttttacg
     4501 tcgtgccaat acttcacgaa ctgtatcgca gtaaccgaat tggaccatac cagggtcaca
     4561 aacgatcatg acacgttcaa cgttttccat tttctctaag tacaatagag agttcttttc
     4621 aaagaagatt tttggcggta atttgaacca ttgcatgtta tttctccgtt tcgctacagt
     4681 tttgacattg attaagttta cagcagagac gttacgagaa acagagttct taccataaga
     4741 accgcatccc aaagtcaatg acggaatcat ttcgttatag atatcaccga tgccgccttc
     4801 tgcagatggt gtattcacta aaatacgaca agctttcatt cgaagaccga atttgacatg
     4861 caactcttcg ttttctgaat ggataactgc tgtatgtcct aatcctccaa gattcaacat
     4921 accttcacac aaatcaaacg catggtccgt gttgttagct ttgatcatgg caagaactgg
     4981 agaaagtttt tctcttgata gcggataatc tgggcctacg ccttccaatt cagcaatcag
     5041 cattttcgtt ccttttggta cactgattcc tgccaatttt gcgatttcca tcgcagaatg
     5101 tccaacgatt gatggattga ccgcatattt tccttcgttc atcactgcat cttctaattt
     5161 ttgcaattca tctggtttta cgatataaac ttggtgtgct tggaattctg cttttactgc
     5221 tgcatagatt tctttatcca cgatcacagc ttgttcagaa gcacaaatca ttccattatc
     5281 aaatgttttc gaaacgatca aatcgttcac tgcacgttta atttttgctg ttttttcaat
     5341 ataagctggt acgttacctg gacctacacc taaagctggt tttcctgtag agtaagcaga
     5401 cttgaccatt ccagctccac ctgtcgctaa gacgatcgcg atacctggat ggttcattag
     5461 catagaagtc gcttcgatcg aaggatgttc gatccattgg atacaatttt caggagctcc
     5521 ggcagcgata gcagcatcac gtaccactcg tgctgcttct gcagaagatt tttgggcact
     5581 tggatggaaa gcaaaaacga ttggatttct tgttttcaac gcgatcattg atttgaaaat
     5641 cgttgtggaa gtcggattcg ttgttggagt tactccgcaa accacaccga ctggttcagc
     5701 aatttcaatc aacccttttt ggacatcttc attgatcaca ccaactgttt tatcatgttt
     5761 gatattatgc caaatatatt cggacgcata catatttttg attgctttat cttcgtaaac
     5821 accgcggcct gtttcctcta cagccatctt cgctaatggc atatgttgat ccaaagctgc
     5881 cattgccatt tcatgcacga tatggtctac tttttcttga tcaaagcttt ccatttcttt
     5941 caatgctgca tttgcttttt ctgccaaatc atcaatcatt gcttgtacgt caattgtctt
     6001 cgttttttct ttttctatta ctttagccat cattatcctc ctaatgggtc atggttattt
     6061 gttaaataat tcacatgtat atcatacaat atttattttt ctatgtaaac actttttttg
     6121 tgattttttt cacagttatt tttaaatata aaattgcttt atttacaggt gtttgtatag
     6181 aaaaatatat aaagttattt attattattt atatgacaca cccgattatt caatattgag
     6241 caatatatcc catgctctta tctctttctt ttttgaataa aaaatgaggt aggacatctt
     6301 tctgccctac ctcgtcttta aataagcttt gatacttatt tttcttcctt ctcttctgtc
     6361 tcttccagcg tttctgttgt ttcagttttt acttcaactg tctcagctgg ttcttctgtt
     6421 gtttcaacag tagtcgttgc atcatttgtt acgacagtcc ctggtttgac cgttttgatt
     6481 gctgcacgat cgtactcaag gaagatccct tcgcagtcta aaacaactgt ccgtttgtca
     6541 ttgtcgattt ctgaaacaac tccatgtaat ccgccgatcg ttacgactga atcacctggt
     6601 ttcatgctat ctaacaagtt ttgacgttct tgctgttgtt tcttttgcga acgtgacata
     6661 aaaaaccaca ttgctaatag cgcgataaac atcacaagca ttggtaatgt tcccattttt
     6721 ttcacctcac taattctgta tctacaaaaa acactttagc agaaaaatgc tgataacact
     6781 agttttttac ttaattttta aaagattttc ctttagaagc tttttgcgtt ttctttattg
     6841 aacccgtatt cttcaaaaaa ggcttctcta aattcaagca aattatcatc catgattgct
     6901 tgacgtactt gtttcattag attcaacaag aagtataaat tgtggtaaga agtcaaacga
     6961 atcccaaatg tttcatcaca tttgatcaaa tggcggatat aggcacgtgt atagttttta
     7021 catgtataac aatcacattt ctcatcaatt ggacggaaat catgggcata tttcgcattt
     7081 ttcactacta gacggccttg agaagtcata catgtgccat ttcgagcgat tcgagtaggc
     7141 aagacacagt caaacatgtc tactccacgg ataacaccat cgatcaatga atcagcagca
     7201 ccgacaccca tcaagtaacg tggtttgttt tcggggatca acggtgtagt gaattctaat
     7261 acacgattca tttctgattt aggttccccg acagataacc cgccgattga gtaaccaggg
     7321 aaatccatcc cgactagatc tttcgcgctt tgacgacgaa gatcttcaaa ccctgctcct
     7381 tggatgattc caaataatcc ttgacggtct ggattagcat gagctttcaa tcctcgttct
     7441 gcccatcgag acgtacgttc tactgatttt ttcacgtagt cgtaactttc atcgaatggt
     7501 ggacattcgt caaaactcat catgatatca gagcccaatt tattttggat gttgattgct
     7561 ttttctggtg ataaaaacat acgcgaaccg ttcaagtgat ttttgaaatg cacgccttct
     7621 tcttcgatat tccgcatatc acttaatgaa aaaacttgaa agccgcctga atctgttaaa
     7681 atcggttgat cccagttcat aaatttatgc agtccgccag cttcttctac taagtcttca
     7741 ccagggcgta gccataaatg atatgtatta cttaaaataa cgccagcacc catttctttc
     7801 aactcttctg gggacattgt ttttactgta gctaatgttc ctactggcat gaacattggt
     7861 gtggggaaag ttccgtgtgg cgtgatcaat tcccctaaac gcgcgccagt atgtttttct
     7921 ttcttgatta aacgatagcg aatggcaggt tgcgtcattc atttccctac tttctatata
     7981 aaatatcttg acaactccca ccatcataat cgtttcaagt gctttttgca agttccctcg
     8041 gcttccttct actttattta atattctttt ttttatcctt tttttatgaa taacaaaaaa
     8101 aagaaaaaca ttatgacaga aatatttttt gatacatggt atattggtgt tattaaagaa
     8161 aaggagggaa aaatcatgca atcttctgca gaattaaaac gccaagcaaa agagagtcta
     8221 aaaggacgtt ggggacaagc tgttttacta aatctaatcc ctacattgat tggtattgct
     8281 actattcttc ttattatgat tcctattatc ttgtttgcca ccaccatata tgatggatca
     8341 gcgagcgtct cagaagcgac aacttcagga ggcggtagtt caggaggcgg tggcggaatt
     8401 atttccagca tcattagcgc tttgttcatg agcggtattt cttggactta tcttgatatt
     8461 ttacgtggac aaaaagatca gatcgaacca ttcaaagatg catttcgtgg attcaaaggt
     8521 ttgtttatcg gcggagtgat cagcttagct gtgctgatct cgatctttac aacattatgg
     8581 ggtttgctat tagttattcc tggaatcatt aaaagctatt cttattctca aagctatttt
     8641 atttactacg atatcgtcca agaaacagga caaacaccta aagtactgga tacgatcact
     8701 gcaagtcgaa aattgatgga tggttataaa ggacaacttt tctggttaga tctttcattt
     8761 atcggctggc atatcttagc attagcaact gctggtattg gttatctatg gctcagccca
     8821 tacatttctg caacaaaagc agcattttat gaacaattgc caaaagatat ttaaaagaga
     8881 gtgtgacttg tgaccgaggg ggtggcagga gaacaccgtt tatcgagagg cagtaaaaga
     8941 aacattcagc ccattagcaa aaaggaggtg tgacaacatt catgtcacac ctcctttttg
     9001 tatagttcca gtaaaaagaa aatcaactta gaaacagtta atcattcctc ttcttctgct
     9061 tccggtaatc gatggaactt gatcagttca ttcaccgcga ttggtacgat agataaaatc
     9121 cctaccaaga accagtgatt caaactgatg tgtactaggc cgaatagttc ttgcgtaaat
     9181 ggcagcaaag caacagccgt tgtaatagct aatgccagta acgccatttc taccaaagct
     9241 ttattggtcg ctaaattgac tttgaagatc gattggctgc tgcgtacatt gaacacatgc
     9301 agaatcgatg aataagctaa gatcaagaat gcaacagttt gaccgacatg ctgacttgcg
     9361 tctacccatg ggctgatagt atcgacataa ctacctaaat aataaccaaa caaggttacg
     9421 atggtgaaga cccctgcatt tatggcaatt ttttgcaata aacctcgagc aaagatccct
     9481 tcatttttcg gaatgggtgg ctgatccatg atgccgtgtt ccgctggctc tcttcctaaa
     9541 gcaaatcctg gaagtccatc agcaactaca ttgacaaaca gtagttgcac agctgtaaat
     9601 ggtgctcccc atcctaacag catcgcaatc aaaacaataa agatctccga aatattacag
     9661 cttagcaaaa agttgatagc tttacgaata ttctgataaa ccgaacgtcc ttgactgaca
     9721 gcgtctacga tcgtcgcaaa gttgtcatcc gtcaaaatca tatctgcggc actttgcgcc
     9781 acatctgttc ctgtgatccc catggcacac ccaacgttac ttgctttcaa cgctggtgca
     9841 tcattcacac catccccagt catcgctacg actgcacccg tacgttgcca agatttgacg
     9901 atccggatct tgtcttctgg cgttacccga gcatagacag agagagcttt gacacgcgca
     9961 tctaattctt cgtctgataa ttgatgcagt tctgatcctg aaagtgcttc tgacggatca
    10021 ttcaagatac ctaattcttt cgctatggca ctggcagtca ccacatgatc tccagtgatc
    10081 atgactgttt tgatgccagc ctttttcgct cgcttgattg ctcctttact ttctggacgt
    10141 ggtggatcga tcattccgat cagaccaatc aaacgtaagt ctttttccag tgcttcagaa
    10201 gaaatatctt gcggttcttc gtcataagta gcataaccaa cagcaatcac tctcagtgcc
    10261 cgtttaccaa agcgatcatt aacgatcgcc gcttgttcta catcgcctga actaaagcgc
    10321 ggagcaagaa catcgaatgc tcctttggtg attgagatgt atttttcgcc catttggtga
    10381 actgttgtca tcatttttcg ttcagaatca aaaggaagct cgtttactcg tggatatttt
    10441 tcttctaatt ctgcctttgt atgataattc tcttcaacag cccttacgat ggctacttct
    10501 gttggattac ctttgataac aaggtcgtca tcttcttgct cgaccgtcac atctgtacat
    10561 agtgcagcca ttttcaatat ttccatcgcg ctgttcgtca ttgaatcttc gatattcgtt
    10621 acttcatctt ctttgctcca tacccggcgg acgcgcattt cattttgtgt cagcgttcct
    10681 gttttgtctg agcagataac attagcggtt cctagcgttt caactgctgg caactgtcgg
    10741 atgatcgcat gtttcctcgc catcttctgc acaccgtatg caagtgtcaa cgtaacaata
    10801 acagtcaagg tttccggtac agctgcaaca gccaaggaaa tcgaagtcat gaacatctca
    10861 aggagtggtt ctccttgcag ttcaccaatc acaaatacta aagccgcagc cgctaaagcg
    10921 atcatactga ttcgtttgcc tagctgattc aatcggacct gtaaaggtgt tttttgcata
    10981 gtattttcgt tcaaaagatc cgcgatctta cccatttctg tagccatgcc aatggcagtg
    11041 acgatcgctt tcccacgacc tgcagcaacc gtacatcctt taaataccat attaagttgg
    11101 tcacctaatg actcatcttc ttcagcaata aatgctgcat ctttttcaac tgcttcactt
    11161 tctccagtca acgccgattc ttctacacgt aattgtgatg cttcgatcaa acgggcatcg
    11221 gcagaaatag catcaccgga ctctaaaaca agaatatcac ccactaccag ttgctctgac
    11281 tcaacagtgg tcactttgcc gtctcgtatg acagttgccg tttgtttatt catatcttgc
    11341 aaggattcca gtgctttttc tgcgtttcct tcttggacaa tcgccaatac cgagttgatc
    11401 acaacgatag cgatgatcag cagtccttca aataaatccc catgttctgt agcaaatgcg
    11461 gtataaaagg aaatcaccgc tgctacaaga aggatgatcg ttgtaaaatc ggacaagctt
    11521 cgaaagaatt ttttgatcat cgattctttc ggcgcctcat cgaatttatt caatccctgc
    11581 ttttttcgcc gttcatccac ttcttgtgaa gacaacccag tatctggatc tgcttctgtt
    11641 accttcttta cttcttctat tgtctgcttg tattcttcca tcctattcct cctttaaaat
    11701 cttcgtacaa aaaaaggtac ttattttact ataacatcct ttttttattt acttcaaata
    11761 aagggcttat cgattatttg ttaagaaagc gtaaaattta aagtgcttat atttgcaaac
    11821 gctaccaaag gatgctatta tatagatgta aggaaggagg ataccatcaa atagacagaa
    11881 gaatttctcg atagaacagt gtaagcaaac aaagttgtta gaagtacagg tttcacccag
    11941 acatgcgtgt agagtacaaa cctataaata gtgaatgcgt atggatgttt ggatcacgaa
    12001 gtatccgaaa caagaagtaa tgcgatacgt aacaattgaa taacgtaaac gtctgaaaat
    12061 tgaataggaa caagagatgt ttcaaatgtt gaagatgtaa cgaaaacaaa atttttgttt
    12121 aacatgaaga aattcaacac cgtgctttgc atgtatgatg gaaagttaca atcatttctg
    12181 tttatttgaa tccttccgag gtacaagaaa gccgagctta tcgagagctc ggctttttct
    12241 ttgtcataaa atctttgtaa agcttttttg ttattcaaaa ctagcggctt ttcataacct
    12301 tatttcacga acatcgcatc tccaaaacta aagaaacgat agcgctcttc aatcgcatgc
    12361 tggtaagcag ataaagtcaa ttctcttcct gcaaaggcac taactaacat gactaaagtc
    12421 gattttggca aatggaaatt agttgagaaa gcttctacca ctttgaattc atagccaggc
    12481 gtaatgaaaa tatcagtcca tccgctgtct gctttgattt ctccattaaa tttcgttcca
    12541 atcgtttcta atgttcgaat tgaagttgtt ccgacagcca cgattttccc gccattttta
    12601 cgtacttggt tcaatcgctc agccgcttct tccgtcaaac ggtagaattc actatgcata
    12661 tggtgttctt caattttatc tacgctgaca ggtcggaatg tacctaaccc tacatgtagt
    12721 gtcagataga ctaattcaac accctttgct tcgatctcat caagtaattc tttcgtaaaa
    12781 tgcaatccag ctgttggcgc tgcagcagaa ccattttctt tcgcatagac agtttgatag
    12841 cgatctgggt cttccagacg ttctttgata tatggaggca atggcatttc tcccaatgat
    12901 tcaagaatct ctagaaaaat tccttcatac tcaaagtcaa tgatccgtcc gccgtgttcc
    12961 aattcttctt tcactactgc ttgcaatctg ccatcaccaa aagagattct tgtcccaact
    13021 ttggcacgtt tcgcaggttt gatcaatgtc tcccacgtat ctccttgtgt attcgttaga
    13081 agaaggactt ctaaatgcgc accagtatct ggtttttctc catataaacg agcaggcaga
    13141 actcgtgtat tattcataac tagcgcatca ccaggctgta actcttcaat gatttcatgg
    13201 aaatgtttat cttccatctc tccagttttc cgatccaaaa caagtaaacg agaatgatcg
    13261 cggtctttta atggtgtttg tgcgattaat tcttccggca agtcaaaatc aaaatcttct
    13321 gttgttaaca tatcgtcact cactttctat catgtgcttc atcattctat catgtttttc
    13381 gttctgttgg caaaatcagc actttttcac ttctattttt tgttttccta aataaaaaaa
    13441 ctaccctatt tgtatttttt cattgtttta ggcatactag taatagatag ttcttcattg
    13501 aaaatagaaa aagaggtggc accatgattg aatttcaaaa tgtgtcaaaa atttataaag
    13561 gtggaaaagt cgctgtagag gacgtcaatc tttcatttga aaagggtgag ttcatttgtt
    13621 ttatcggtac gagcggaagc ggaaaaacga cgacgatgcg gatgatcaac cgaatgactg
    13681 atccaacaca agggaaaatt ttgattgatg gaaaatccat ccaagaaatt gatccagtgg
    13741 aattgcgacg tcagatcggc tatgttatcc aaagtattgg attgatgcct cacatgacga
    13801 tccgtgaaaa tatcactctt gtccaaaaat tgttgaaagt cagtcaagaa gaacggaata
    13861 aaacagctga aaaaatgatt gatcttgtgg aattgccccg tgagatgtta gatcgctacc
    13921 cacatgaatt atctggtggt cagcagcagc gtattggagt cgttcgtgcc cttgcagcga
    13981 atcaagatat catcttgatg gacgagccat tcggtgcgct tgatccaatc actcgtgatt
    14041 ccttgcaaga ccttgtcaaa gatttacaag agcgtttggg aaaaacgatc gtatttgtaa
    14101 cccatgacat ggatgaagc
//
LOCUS       DANKEF010000060        14016 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000060 DANKEF010000000
VERSION     DANKEF010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14016
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.060"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(538..900)
                     /locus_tag="PWZ48_002194"
     CDS             complement(538..900)
                     /locus_tag="PWZ48_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261840.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(974..1354)
                     /locus_tag="PWZ48_002195"
     CDS             complement(974..1354)
                     /locus_tag="PWZ48_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HDL2261841.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1418..2611
                     /locus_tag="PWZ48_002196"
     CDS             1418..2611
                     /locus_tag="PWZ48_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2261842.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2688..3272)
                     /locus_tag="PWZ48_002197"
     CDS             complement(2688..3272)
                     /locus_tag="PWZ48_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2261843.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            3473..4471
                     /locus_tag="PWZ48_002198"
     CDS             3473..4471
                     /locus_tag="PWZ48_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2261844.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            4583..5092
                     /locus_tag="PWZ48_002199"
     CDS             4583..5092
                     /locus_tag="PWZ48_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDL2261845.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(5122..5781)
                     /locus_tag="PWZ48_002200"
     CDS             complement(5122..5781)
                     /locus_tag="PWZ48_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HDL2261846.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(5783..6547)
                     /locus_tag="PWZ48_002201"
     CDS             complement(5783..6547)
                     /locus_tag="PWZ48_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HDL2261847.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(6555..7232)
                     /locus_tag="PWZ48_002202"
     CDS             complement(6555..7232)
                     /locus_tag="PWZ48_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HDL2261848.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(7269..8654)
                     /locus_tag="PWZ48_002203"
     CDS             complement(7269..8654)
                     /locus_tag="PWZ48_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HDL2261849.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(8864..8948)
                     /gene="ffs"
                     /locus_tag="PWZ48_002204"
     ncRNA           complement(8864..8948)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PWZ48_002204"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(8971..9486)
                     /gene="tadA"
                     /locus_tag="PWZ48_002205"
     CDS             complement(8971..9486)
                     /gene="tadA"
                     /locus_tag="PWZ48_002205"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDL2261850.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            9593..10450
                     /locus_tag="PWZ48_002206"
     CDS             9593..10450
                     /locus_tag="PWZ48_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261851.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(10531..10866)
                     /locus_tag="PWZ48_002207"
     CDS             complement(10531..10866)
                     /locus_tag="PWZ48_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261852.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(10933..12303)
                     /locus_tag="PWZ48_002208"
     CDS             complement(10933..12303)
                     /locus_tag="PWZ48_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HDL2261853.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(12381..12917)
                     /locus_tag="PWZ48_002209"
     CDS             complement(12381..12917)
                     /locus_tag="PWZ48_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2261854.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            13226..>14016
                     /locus_tag="PWZ48_002210"
     CDS             13226..>14016
                     /locus_tag="PWZ48_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261855.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERA"
BASE COUNT         4277 a         2798 c         2281 g         4660 t
ORIGIN      
        1 cccgattcgt ttttggctgc cgtagcaact tttatatcat atcagagaga caagtaattg
       61 tcaactcttt tttaaaagtt tttttgattt cgtttttcga aatctttcag caactcattt
      121 atcataactt atcgtttgtt atttgtcaag aacttttttg aagttttttt cgatagaatc
      181 aagcttcgca agtggttctt ttttaacgac tcagttatct tatcatgtcg ttagtaacat
      241 gtcaataact attttttaaa ttttttagaa atgtgatata acatccactt cagaatcaac
      301 gctgttaaat ttatcatgta tttcttcctt ggtcaactat aaattaagca ttactttgtg
      361 aaatgatttt tcgatcaatt attattattc gttatacaac cgttttcaac atattttgaa
      421 aataaaatgc actaaaaaac tatctttagc agaacatttc attgtatttt ttggaccaaa
      481 caataaaaaa agaagtttga aaaaaattca aacttctttg ttcgcttaat ctgtatttta
      541 gaagaaaggc actccctcta cttcgtaagt tccaagaaca gagcgcattt gacctggttc
      601 ttcccaaata tatccttcgc ctaattcttc tctttgattc ttttgttcag tttcaaagac
      661 aaacaacgga acattttcct ctttaatatg tgtatttgtc aattccatca aactaatctt
      721 gctaacatcg atatgaacta tttcttttaa gaaattaagt atattcgcta aaccagtctt
      781 tccttcagaa agttccgcta acgcaaattc caacttgtca tcaattgaat ggaccaaaaa
      841 ttttttagta ccatcctcca aatgcaacat aattgtacca gcaactcttt tgcttgccaa
      901 aatttccacc tcgatcaact tgttctatgg cttacataaa aattctagca cattttcaga
      961 aaaaatacac tttttatttc ttattttctt tccgttctag ttcagataat gcttcttcta
     1021 tccaaatagg taactgctcc gcaatagtgg taaatccgat cttacgattt ttattcgtga
     1081 aatacctttt tctgcgatat ggaacagctg gatttgtttt ttctaacgtt cttaaagata
     1141 aactgatttt tttggaatat tcatcaatgt cgatgatctg aactgttact ggctgtccga
     1201 cttttaacaa tgaatggata tttttcgtat aaccggattg tacttctgaa acatgaataa
     1261 gtccttgcgt ctcgttatca agagaaacga atgcgccata aggctgtatc cctgtcactt
     1321 ttccttcaat tatttgtcca attttataag tcatctactt ccctctcttc gcatttatca
     1381 ttatactgta aatagctcaa aaaaagaagc gacgaaaatg actggaggtc ttaagatgat
     1441 cgaatttgat aaacagattt caagaaaaga tacagaaagt gtaaaatggg acgcaattgc
     1501 tgagacatat caaatggatg acttacttcc tttgtgggta gcagatatgg acttcttgtc
     1561 tcccggtggg gtagctaaag cgtttagtga atacatcaaa catggaatct ttggttatgc
     1621 cactctgtct gataaactat atcaggctat cattcattgg gaacgccaac accatgctgt
     1681 ggagttgacg aaagaaaata tcgtttttac aagtggtgtg ttaaccagtt tagctgcagc
     1741 agtacaaact tttacaaaac caggcgattc tattttgatt catgacccag tttatcctcc
     1801 attttcctct attatagaaa caaatcaacg tcacatcgtt cgaagctctt tgctagaaaa
     1861 aaataatcat ttcatcatgg acttgtctga catggagaaa aaaatcgaag aaaacaatgt
     1921 aaaagcaatg attttatgca atcctcacaa tcctggaggg cgtgtttgga gcaaagaaga
     1981 attgaaacag ttaagcgaat tgtgtttaaa acatcaagtg attctgttta gtgacgagat
     2041 ccatcaagat ttaactttat tcgatcatac atttacttcc atgctaacga ttgatcctaa
     2101 tttagatgat ctactagttg cttttacttc cgcaaccaag actttcaatc tagctgcaat
     2161 caaaaattca atggtattta tcaaaaatcc agagatgaaa gctgattttg aaaaacattt
     2221 agtgatgaac caacagcacg aaatcaacac ttttggactg attggaaccc aagcggctta
     2281 tgagacgggt ggagaatggt taagccaact gattccttat ctagaaaaaa atgttcaaac
     2341 agttaatcag ttttttgaac agtatcttcc gaaagtgaga ataatgaacc ctgaaggaac
     2401 ttacttaatg tggttagatt ttaccgccta tatttcagac gaccaagaat tggagaacac
     2461 tcttgttcgg aaaggaaaag tcgtattaaa tcctggcatc acgtttggtc cttccggtca
     2521 tgggcatatg cgcttgaata ttgcttgtcc agaagaaact ttactggaag gacttaatcg
     2581 aatcaagtat gctttagaga caatcgatta aatcatttat tcaaaataaa aggacgagac
     2641 cttactaggt ttcgtccttt ttcataatta ctccgtacca taccaaacta ttctgaaatt
     2701 tcaattgttt caatcactac atcatgtaca ggtcggtctt gtggaccacg ttgtacattt
     2761 gcgatttcat ctactgtgtc catcccttct agtacatgtc caaagactgt atgtcggaaa
     2821 tctagccaag gagttccgcc acctttatac gcttcaatga tttctgatgg gaatccagca
     2881 ccttctagtt gtccaagcat attagctggc acatttgtgt tatttacaat aaagaattgg
     2941 cttccgtttg tgtttggacc tgaatttgcc atagataaag ctccgcgaag gttgaacagt
     3001 tcacgactga attcatcttc aaaactttcg ccataaatac tttcgccacc cattcctgtt
     3061 ccagttggat cgccgccttg gatcatgaaa tcaggaataa cgcggtgaaa aatcacgcca
     3121 tcataataac cttttttgga aagttcaatg aaattttgaa ccgtcttcgg tgctagttca
     3181 gggaataatt gaacagtaat gtcaccacgg tttgttttga taacagcttt tggtcctttt
     3241 tcacttttta aatctagttg tgggaatgcc atattgtttc ctcctaatat ttgcatactt
     3301 cataataata ataacataat acctaacctc tcgtaaaaat actagatttg ttttctttta
     3361 tttttcatca ctagatacat tctataactt accgtttcga tttcgaattc aaacattttt
     3421 gagaagcatt ctcaattaaa ttctgatata gtgtgaaaag ggaggaattt ttatggaaat
     3481 ctatgacatt acaatcatcg gtgcaggtcc tgtagggatg tttgccgctt tttacgcagg
     3541 aatgcgccaa gctaaaacaa aaatcattga cagtctacct caacttggtg gtcaattagc
     3601 aactttgtat cccgaaaaat atatttacga tattcctggg tatccagcaa taaaagcaag
     3661 tgaattaatc gatcaattag aaaaacaatt aactactttt aaccatacat tccatttaaa
     3721 agaagaagtt ctctcgctta ctcgtgaaga cgaagttatc gagattacta caaataaagg
     3781 catccattat tccaaagcag tcattttagc tttaggaaat ggttcattcc agccaagaaa
     3841 attgaattta gataatgctg aatcatttga aaaccacgga ttggattatt tcgtgaatga
     3901 tttgatgaag tatgctggaa aaaaagtcgc tattgctgga ggtggtgatt ctgctatcga
     3961 ttggacgttg atgttagagc ctattgcaag cgaagtttac ttgattcatc gtcgcccgga
     4021 atttcgtgcg cacgaacata gtgtatcacg cttgaaatct tcatctgtca acttgcttac
     4081 tccctatctc atcgatggat tatcaggcaa taatggggaa ctgacagata tccggttaaa
     4141 aaaagtgaaa agtgacgaga cgattgattt gatgattgat tctctgatcg tcaattatgg
     4201 attctcttct aatttagaac atctttcttc ttggggactt gatagtacca gaaatgccat
     4261 tactgtaaaa tcagatatgt ctacttctat tccaggcgtt tatgcagctg gtgacatctg
     4321 tacctatgaa ggaaaagtga aactgattgc tactgggctc ggtgaagcac ctactgcagt
     4381 gaacaacgct ttacactata ttaatccaaa ggaacgaact cagcctggtc acagcacaag
     4441 cttatacgat aaaaatcttc gtcctagttg attttttata tctctttgca tttcaagtta
     4501 aacccgaaag cagtttcttg catttttttc atgttccgtt aacatgaaat agagaaagaa
     4561 aaaataaagg gggagcaata atatggtcgt caaaactgag actttagaag aaaaggcaag
     4621 agaactattg aaggaacgtg gcgtaactgt agaagatatc gcacaacttg tcatgttttt
     4681 acaaaaagac tatatttctg atttgacttt ggcggattgt actgaaagtg tcaacagcgt
     4741 gctaacaaag cgtgaagtac ataacgccat tatcactgga atccaattag atatattagc
     4801 agaaaagaaa aaattgatga gtccgttgca acatattatc gaagatgacg aaggccttta
     4861 cggtatcgat gagatcctag ccttgtcaat cgtgaatgtt tacggtacga tcggctttac
     4921 gaattatggt tatatcgaca aggtgaaacc tggtatttta aaagaactaa acagccacga
     4981 tggcgttcat gtccatacct tcttagatga tatcgtcgga gcgatcgccg ctgctgctgc
     5041 cagtcgatta gcacacaaag aaccagaaaa aagaagtcgc cttactcaat aattcaacaa
     5101 aaaaagaaag aaaacgtcta tttagacgag ttctttcttt tttgtttgtt tcaagctatt
     5161 ttttcctaag aaaagaaaaa cagcaaaaaa taattctaat aatacaaaaa ataagataaa
     5221 gctatgcatg aaaaattctt ctggaagtac attgatgatt ggatcagtaa cgggatcgaa
     5281 taaccagtca tcgttgttga aaaacgtttc atgaaaaagg ataaaaaagc gatcaaatcc
     5341 tatcatcatg aaaaaaccga aaacaactgg cagtaacatg ccgatttgaa acggacgaat
     5401 caaccgccaa agccgatcat tcttttgcaa atatttgata aacaaaatac tcgggatcaa
     5461 agtgatgaaa aacacagcat aatccactaa aaacaaaatt ttcacttcat aaaagtgatg
     5521 ggctccgcta gcagaaactg ggaaatctgg caagctgagg atggttttga agggattatt
     5581 gagataattc attaggtgat caaaattttt cagtaacgtc tcttgatcta aagaagtata
     5641 atccaaaatt tgcaaatgtc cgatatcaaa tacgtatagt ggtcgaaagt tgatcgtcac
     5701 accaactgcc agactgatca gtgtcagaaa taggctgaac aatccagcat attccatcca
     5761 ctgccatctc gaatttttca tattagaagc tccattcatc cagtgattgt ttcacatagg
     5821 tagctgggat aggcaaccca ggtacatctt cttcttttgt aaaacctgat aaaacgagca
     5881 aagtatctat attattgcgt atcccagctt ggatatctgt ttcatagtta tctccaacca
     5941 tgattacttc ttctttttgc agccctaata cttcaacggc tttatccata atgatggctt
     6001 ctggttttcc gatgtagatc ggcggcgttt gggttgctgt ctctacaaaa gagatgactg
     6061 accctgctcc aggaagaagg ccgcgttcag taggtatatt cttatctggg ttcgttccaa
     6121 tgaaagtagc acctttttga atagcaagtg ttgctttaac tactttttca taggtcagat
     6181 aattatctaa gcctaccact acatagtcag gagcctcttc ttcccatgta aatccggcag
     6241 ctaaaatcaa gtcaatcaat cctgcttctc cgataacata gacttttttc ccttttccat
     6301 ctgctttcat aaaatcgatt gttgccaaag tagctgtgta gactgtctct ggagctacat
     6361 gaatgtcaaa ctcatctgct agacgattag ctactgtttc tggtgactta gtcgtgttat
     6421 tggtaacaaa caagaaaggt aattttcttt tttgcaattc ttccacaaat cgtttaccgg
     6481 ccggtatggg ttgtgtccca cgataaatcg ttccgtctaa atcaattaaa tatcctttat
     6541 agtccattca tccactactc ttcccttttt ctgatcgtaa atgctgtttt tgttttttct
     6601 acttgtttag gttgttcttg tttttttact ttttcagagg tttttgctga ctctgttttt
     6661 cgatttttaa taaccggttt tttgcgatta gatgcgactg gtcctttttt ctcatcgata
     6721 tacgcacgat tatggttttt tttctttttt cggcgctgag ttgtcttttc ttttttaccg
     6781 ccgattctct cgatgacaaa atacgcacaa ccaaaattgc aatattcgta aagatagtct
     6841 tccaaggaat cgattcgctg atctggatgc gcccgtcgat tatccgcttg gaaaaatcct
     6901 tttaggcgta gttgttcata accccaatct cctacgatat aatcatatcg ggctagtaca
     6961 tcactgtaac gttctccaag cttttctgca ttaaatcctt cacgataatc ggacacaagc
     7021 cgataatgtc tttcacctat caaaaaatta ctgtcatcta acagtgtcac tagctcgcct
     7081 ttgacttttt ctgctttagg ctcctcaacg atttcttcca aagcagctgt cagttcttcg
     7141 gtgactgttg tttcctgttc agctgatgat tttttcttag caatttctgt tttatgctca
     7201 acgttctgct gttcattttg ttttgcagac atgcgttcac cacctatctt ttttattata
     7261 ccttgttttt atttgattgg gtagtgagct ttcaagtaat ctcctaccac acttcggata
     7321 aattcaggga acaaaaattc attttttcca gcaatctcaa tcgttggaaa aaatggaaca
     7381 aacatcaaat gatctacggt agtaaaagta taatttttat tcagatcgat tggcttgttc
     7441 ttccaaaaga cttgatggtt aatagaatca tatgtgattc cattatagac catctgccca
     7501 aaaatcttac cgcgaaatcc cattcccttc ataggatagt tacgtaaaaa gttacgattt
     7561 ttttctattt ctaaaactaa gcggatcaga tcatttccac ccaatgtgac atttaacaag
     7621 tgcatcggat gcggcaacgc agtgtgaagc tgatcctgat tgacccgccc ttttggaatt
     7681 tcagttaaaa acagaccact gtttaatatt gctgcttccg tctttcctcg ttctttcacc
     7741 gcctcaaagg ctacttggat aaacgaatgc tcattgaaga tatctagact cagagaaaat
     7801 ggcaaatctg ctacttcttt ttcttttaat aattcatgcc cacgttctag ataactattt
     7861 atttcttgtt catcttctgt aaaagctgtc attgtttcgg tagggatggc tttggctgaa
     7921 tgcttaagga tttttttctc ttcatcgatg gttaaatgga cttcaccgac atactgcccg
     7981 tactttccag cggctgctat ttggacatca ttgattacgt gaccttctct gaataaatga
     8041 tgggtgtgcg atcctaaaat aacatcaatt gctggtattt cttttgcaat ttggaaatca
     8101 tcctgaatcc cgagatgact catcaaaact aaaacatcca cttgtgaacg taaatcttcg
     8161 actaattctg gcaaaatatc ataaggattc cgtatgtccc agccattcgg attataagta
     8221 agcggaaatg gagcagtaaa tgcaattagt ccgattttcg tctggtgttt cgttgtaatg
     8281 attttatatg gtttcgtcca tgccggtggc tggagcgtat ttttatcgaa taagttatcc
     8341 aggatgatat caaactcagc atgatcgtat aaatgattca actcttcttt ggaatttcct
     8401 acgccttcat tgtttcctat cgtcgctgca tcataaccaa cttcattcat cagcaagata
     8461 ttttcctgtc cattcgttgc ttctgacaat ggatgccaac gatccacaaa atcacctaga
     8521 tctactgcaa tcgttgtcgt tttttctttc ttttcgtttt ctcgtttacg ttgatccaaa
     8581 aaccttctga tccttggcca attttctaaa tgagaatgca gatcattcgt atgtaaaatc
     8641 actatttttt ccatgtaccc tctcgcctct ctttatcttt atcataccat aggcagcttt
     8701 ttcttccatt gaaacaagct ctatttcaga gtttttcctg ccttgaagca aattgtataa
     8761 gtacaatatt cgataaatca gtctggctaa aaaatcagga acattcattt ttacagtact
     8821 ataaaaataa aaaaaacaga cccgaaagtc tgttgtcgtt tcattataat cagacacatg
     8881 gcggaacctg cttagtgctg cttccttcca gacctgacac gcttcacaag cccaccattg
     8941 tcctagcctt tcggcaaaga atagtatacc ttatttttca ttcgttgacc agtctttttt
     9001 taatttcttt tttcgctggc ggagctttcg gaaaaagtca gtcaaaatca tcccacattc
     9061 gtcttctaaa atgccacctt ctacatatgc ttggtggtta aaccgctcgt cctccaaaag
     9121 attcatcaag gtaccggctg ttccgccttt tggatcatac gcgccgaaat aaacttctgc
     9181 tacacgagac aaaatcatcg cgccgctgca cataggacaa ggctctaagg taacaaaaag
     9241 tgcagcatct tccaagcgcc agcttcccac tttttcacag gcttccttga ttgcaagcat
     9301 ttctgcatgt gtagtagcat cttgcgtggt ttctctaagg ttatatcctc ttccaatcac
     9361 ttttccatcc aatacaacca ctgctccaat cggaacttca tgaagcattt ctgctttctt
     9421 tgcttctgcc aatgcaaggc gcatccattt ttctttttct actgcatcaa gatttgtatt
     9481 taccaattac ccttccctgc ctttgttttt tcttgtttta ctttagcata tgataaaaag
     9541 gctgtaacta actttttttt ctatggtaaa ataaatacaa gaggtgaaca agatgaaaat
     9601 ggataaatta cttgctcttt acccacaagc acagaaaaaa aactctccag catcagaagc
     9661 ggatatcctt tcttttgcaa ttgatgatgc ttttatctgg atcaaacaag attcattatc
     9721 tcaacaagaa accagccttt tgaaagcgtt attccctgta atcaacgatc aaaaaaaaca
     9781 cccatggtat cactatcttt ttaaagatgc ttcttgtgtt caagaaaaaa gctttcgttt
     9841 gatccagctt cacgtcgaat caaaaggaga attcttaaaa aaagaatggc aagatactat
     9901 tttggaatta tttcgctatt ctgctgattt ctttttttat acagacacag atgcgctctt
     9961 agtcgaacaa ataaaccgtg attatcttga tgctactgaa atcaacggta tttttctatc
    10021 tttagatgct gattttgata cgacaacgtc tgtattcgtt ggttctttcc attcacctgg
    10081 caccgacctc gtccctttgt ttgctgaaga aagaaaaatt tttttaacag agcaaaacaa
    10141 tctttatact cacagccgta ctttttctat gcaagatgtg gctttacatt actatacaaa
    10201 agatactgta aaagatagta agttgatcaa aagttacata gaactgatca aacaaaatga
    10261 aatggaagtg atcattcaag aattatggaa tgaattaggc aatgtcagct ctgctgcaaa
    10321 aacactgttt cttcatcgta atactctaaa atatcgaatc gaaaaatttc aagaacagtc
    10381 tggtttcaat ctaaaaaaag ccaacgattt actgttttgc tatcttttat tgcttcatga
    10441 acataattaa agatagatta aaggggttgt gacaataatt ttgtcacaac cccttatttc
    10501 gaatgaatag tgttcaaaag ctagtttctc ttacatcatt ttttcaacaa aatgcattag
    10561 ccggtctttt acatcttcgg ttgaatcttt gattgaatca ccataaacag agattttctt
    10621 ttttcctgat ttgatttttg ctaacattcc catcaaagaa tccaagtcac tatctgaaag
    10681 accatctacg atttctagta atttttcatt tccatcaaat ttgtcataga cgaatttttt
    10741 tactttcgtc cgagtagtca tatgatggat cttttccatt acttttcccg atacaacgac
    10801 tgctgtcgct acactggcag cggctgctac gcttaatccg attgttactt ttgttgatgt
    10861 tttcataagt atcagctcct ttttatctat ctccatcata gcatagacaa aaaaataaac
    10921 gaactaatcc actcagtgaa atctcaatcc tttagggaaa aagttcttta ctgttccttt
    10981 tacttgtttc ccaaatccga caggtatctt atctaatact agcaaaaccc atccttgatt
    11041 cccatcacgt tgaaatgttt ctcctgctgt gtaagattgc cattcctttt gagtgatcgg
    11101 caagcaaggt atattctcta tcttttttgt agcaagcgct aatgcatagc taggttcaaa
    11161 tcgattcttt ttgaaatctc ctaaatgcaa acctgtacgt acgactttta aaccatcgag
    11221 agaaggggct aactcaggaa cttcccaaag atggtcgttg aacactaaaa gtcttccagt
    11281 agcttcataa tgaaaatcat ttgaaaactc cgtccagagt ttttcctgct cttttgtcat
    11341 ttgcacttta cttttttttc gtgtcttctt ttctttatgc atctgatttt gtccatgaaa
    11401 agtcagcttt gcaacaaaat gtccttcacc ctgatcttta tgaggccata tacgtattgt
    11461 tttttccaaa ccagctacgc ttccccattc agaacgtcct gaactaacgg attgagtcaa
    11521 aggaatctcc tcaatcgtta ctggataatt ttctacaagc cagctaatga tctcttcatt
    11581 ttcttctggt gcaaacgtac aagtcgaata aatcagttgt cctttatttt ttagcatttt
    11641 tattgctgaa gacaaaatct cttgctgacg tttttgacag tatagcggag attcttctgt
    11701 ccactctttt attgcatttg gatcttttcg gaacattcct tctcctgaac aaggagcatc
    11761 aaccacgatc cgatcaaaaa agcccgaaaa atgagggaca agttctgcgg gtgcatgatt
    11821 tgtcactatt gcgttcgaaa ctccccatcg ttctatattt tctgataaaa tcttcgcacg
    11881 tttcggaaag atctcattcg tcactaataa ccctttcccc ttcatttgag cagctaattg
    11941 ggttgattta ccaccgggag ccgcacacaa atccaaaact ttttcaccgg gttttgctgc
    12001 agcagctgtt ccaacgatca ttgcacttgg ttcttggcta tattcatacc cagcttgatg
    12061 gaggaaagac tttccattga cagtgccaag aaatccctca ttgctgtagg gagcgggttg
    12121 taattcttcg ctatggtaag tttggaccat gtccaatcct gccggtttta gtggattcca
    12181 ccgaaatcct tttttcacgc ttccttgttc aagcgcagaa aaaaaatcgg atgcttcttc
    12241 gcctaaaaga agacgatatt ttttgataaa ttgctgtggt aacgttgtcg cttcctcctt
    12301 cattccaatg tttcgttaga cattttacaa tcttttttac gaaaaataaa ggtggctgat
    12361 ctactttttc atgttgctgc ctaaggtgac tgttccttta agatattttc caagtattca
    12421 tccgtcacct cgtttttatc ccaaacagcg ttcatcattt caagaaaatc ttgagaagaa
    12481 tcatctgagc gcaaacttcc caatgcatta agccatacca attcaagcga cttttcttct
    12541 ttgatcttcg tgtgaccttt gacatatgag aaaaatggcc gcttaaatag cgaatccatg
    12601 tctaaatcag attttgcaat gatttcacga atactttcat ggatagaaaa aaaatctttc
    12661 aataagtaga caatgcttaa aacatcatcg gaactgcatt cagctaagta gttttcaaaa
    12721 taaacagtga tttcttctaa tatcttctct tttaacccgc tgattgattt gaaatgtgta
    12781 taaatcgggt atgtagaaca accgcagaac tctgctaact ctcttgcagt aattgacttc
    12841 attccatatt cttccacgta taccatactt ttgaacagca aatattcttt actataattt
    12901 gttactctcc ccatcattct tacctccgtc tcttttcatc tacccataaa taaataacat
    12961 aaataattct aaaaatatta ttgataatta tcaaaacata tttagttata tccattaaaa
    13021 atcaattttt ccgccttaaa aacaagaata tcttgacaaa ataccctagg aaataaacaa
    13081 tagaataaac aaggtaacaa aaaaattaaa taacgttata taaaaaagaa ggcgacactc
    13141 tgtgagaata acacagactg aaatgacttt ccactcacag tttttgtcta actatataca
    13201 aaaacagaat aaaaaggagg tatttatgct aattgaagca agaaaaaaag agttacttgt
    13261 tctacaaatg gtcattcaaa aaaataattg ttctttacaa gaattatcca atgacttaca
    13321 tattccaaaa cggacaatca aagaactgat ccgaaaaata aatgtatcac tcaagcaatt
    13381 tttattgatt gatgaattta tttattctaa tcataaagga gagatccaaa ccaaagaaac
    13441 cgatcaaact aaaaagttag ctgttttttc taagttaaaa ttatgctacc taaaagaatc
    13501 caatcgtttt aactatttga tgttactaat caattatcca caaactgctg taccaaaaaa
    13561 atatttactt gaacaacttt atatatcgcc ctcctatctc gaaaaactga caaaacagct
    13621 caatcattcc ttaataaaat tccatttacg gatcctatct gtaaaaaatt gctgtactct
    13681 tgaaggaaat gagttatcta tccgtctcta tctttacttt tttctttcag atacatttgg
    13741 aggtaacgat tggccatttt cctcacttga attaaatgca ctaaaaatga acagtacaga
    13801 tgagcaaata gaaaaaattt caaaacaaga aaaaaatatc tacttgctgt taactttatt
    13861 catattacga aacaaacaac aggcatattt tccaaaaact tcttctgaaa tcactgaatt
    13921 gatggactta ttgcagcata cacatgatta ttcagaatac tttacttctt tttcagtgag
    13981 tcgcccttgt gaagagacag aaacagagag agccta
//
LOCUS       DANKEF010000061        13982 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000061 DANKEF010000000
VERSION     DANKEF010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13982)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13982)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13982
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.061"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(142..1086)
                     /locus_tag="PWZ48_002211"
     CDS             complement(142..1086)
                     /locus_tag="PWZ48_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2261856.1"
                     /translation="MDTILITGGAGFIGSTLANYLGKENKIVVVDDLSMGKKENLDMD
                     KQVTFIEGDVSDSQLMERIMKEYQFAYIFHLAAVASVADSVERPVETHRVNFDSALLL
                     LELARKYQSNLKRLVFSSSAAVYGDEPTLPKKEESVIRPLTPYAIDKFAAEQYVLDYC
                     HLYDVPTSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQLAGEKAEFTLFGDGSQSR
                     DFVYIEDVIQALLLVAKEEKALGQQFNVGTGKSTTLLELIHSIDQILGTELALKYEAE
                     RSGDIRDSLADISKIRSLGYQPKFDILSGMERYLKTEI"
     gene            complement(1220..2611)
                     /locus_tag="PWZ48_002212"
     CDS             complement(1220..2611)
                     /locus_tag="PWZ48_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HDL2261857.1"
                     /translation="MLNERFLKYVKGWYFIYFGFILMLRAIGPYLLLPTRYDTLLFWP
                     AGIAGVVLIIIDAIIAFREKNLKKYDWLLIAFIGAMLVSSLMNLKYGYSENIKLIMWQ
                     LIFFFMIYQFGKDYSSNFFFKVFTWILSLVWFLANLVSLYWFVIQYGIKIPIKYKYYP
                     VRLGWLGNRLFGVYTDPNFGAVISLIVIIIAIYYLVTYKHLNLLFKIFFVINIFIQFS
                     FIALSGSRTTLVCLLITTFVFSFFTSYSSRFLKTNTLKRWLTALVVGLLSIIAMYYVT
                     DITAHAWAHVPHITEVKKDTSDSSGTTSSNKVDLKRPDTDSGDVSNLRFELWKSAIEI
                     FKSKPISGGSPGYYIEYAHDKLPYTLMGKDTLTAHNFIFLVMAATGGLGLLTLLTFLF
                     IQCFKALRYCFTQPNLVKDKHFFAILMILTIAVSALFITELILVSTIGSFIFWGYLGS
                     IQKTISKEEGVTN"
     gene            2737..3147
                     /gene="tagD"
                     /locus_tag="PWZ48_002213"
     CDS             2737..3147
                     /gene="tagD"
                     /locus_tag="PWZ48_002213"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HDL2261858.1"
                     /translation="MKRVITYGTFDLLHYGHINLLKRAKQYGDYLIVALSTDEFNWNA
                     KQKKCYFSYEKRKQLLEAIRYVDLVIPEENWEQKVKDIKEYHADYFVMGDDWAGHFDE
                     LEQLTDAQVIYLPRTPEISTTQIKNELNSLNSDK"
     gene            3323..3736
                     /locus_tag="PWZ48_002214"
     CDS             3323..3736
                     /locus_tag="PWZ48_002214"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2261859.1"
                     /translation="MISISHSIKELFTIKDKNIEINSFQETFYKDKKAIVIEGPLVRT
                     FRHCLFCKESAKQIEKNGKKISMIILNRSGNKQTYLRLRKQHYHCQSCGKYFTAHTYL
                     VAPSCFISKQVHYTILEELTERQALKTIAKRCDFQ"
     gene            3893..4150
                     /locus_tag="PWZ48_002215"
     CDS             3893..4150
                     /locus_tag="PWZ48_002215"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2261860.1"
                     /translation="MTFCPAGEKKDLIAYFMRFTRKAHMGVRYIVTDMNAPYFSLVKE
                     PFPNAQIIIDCFHIGQHLNKFFDRVRKRVMKQLNQKCQRHL"
     gene            4237..5475
                     /gene="istA"
                     /locus_tag="PWZ48_002216"
     CDS             4237..5475
                     /gene="istA"
                     /locus_tag="PWZ48_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL2261861.1"
                     /translation="MRKDIREGVMIYVINEIKPNYAALAKQYDCDYRTVKHAYEEAQV
                     KESKPPERKKRPSKLDPYREIIQDKIKDQCSAYSIFKFIEHKGFGGSYSLVKTYCRQI
                     KQAKVHKATVRVEHSPGLSAQVDWKEDMRLISNQGEIFEFNIFLYVLPYSKKKYVTLT
                     FDRKQDTLFECLDDAFYQTGGVPKEIWFDNMKTVVDHSRSQFSKPHFNKRFYAFSKDA
                     GFTPIACRPFRPQTKGCVEALARTVDRLRVYNHEFYDAVELCHLVNNLCVELNNEVSQ
                     STDQTPDERWQTNEKEHLHPYQKNLLNPYFEDFIRRKVTKEEMVIFRKCRYSVAPRYI
                     GKEVEIELSDKEDQVQIYYNGELIRSHPLTTKQLNYHEEDYFQILKSDVMSHKEDDGI
                     RAYTRESLKQYDLVEVADEE"
     gene            5465..6205
                     /gene="istB"
                     /locus_tag="PWZ48_002217"
     CDS             5465..6205
                     /gene="istB"
                     /locus_tag="PWZ48_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL2261862.1"
                     /translation="MKNSHYQELMTNLETLGLNHMREYVPNYIEVANREELSLTEALL
                     ELTRREVDHQNKQKMNRVVARARFSKRTSLEEFDFAFQPSINKREVLDLRHMGFVEKH
                     ENLVFIGSPGVGKTHLTIALGIEACRQGYRTLFITCHELLLRLRTAYEKGTLERVLKR
                     YARYDVLIIDEIGYLPIQKQEADLFFQLLAMRYEHRSTIITTNIVLSRWGELFQSSEI
                     AAAILDRLVHHVKVFKITGKSYRMRDKI"
     gene            6615..6779
                     /locus_tag="PWZ48_002218"
                     /pseudo
     CDS             6615..6779
                     /locus_tag="PWZ48_002218"
                     /inference="COORDINATES: protein motif:HMM:NF013753.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6846..8273)
                     /locus_tag="PWZ48_002219"
     CDS             complement(6846..8273)
                     /locus_tag="PWZ48_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HDL2261863.1"
                     /translation="MKKTVKDIFYNAIYQVFLIVLPLLTIPILSRRIGSTGLGIYGYV
                     FSISQFLMTVIAVGMNPFRIRNIAKSRKDKKALSLQFWNIYFLQFLIGLSVSFLYIAY
                     IYFFDVKYKNLYLIQLIFIAGLTLDISWFFQGIEEFSKVVIRNTVIKLSSVLLIILFV
                     STKDDLWIYVFITSITNFLGSLVFWLSIHGKVGKPLFNSNVFKQLWKPAFIILTPQLF
                     MQVYTTLDKTIVGYFVDPTALSYYDQSQKIARIILAVLTSITIVMLPKISKAQAEGKS
                     ENILVYTKKSFDYTIIIALILFSVVFINTKEFVPWFFGENFKPMTINMLISCLIIVVS
                     PIGGVFANQFAIAIEKDKEYAYPMIIGAIVSIIGNVAFVPTYGAVAATIVLVVVELLV
                     FFFRVFFVRKEIKISYFFGKNVVLFFIVNLTLTTSAYYFLPQLFSSSFLDMAIKSIMI
                     FIIYLPFVYFLFPDIRNEVRKIIRK"
     gene            complement(8270..9412)
                     /locus_tag="PWZ48_002220"
     CDS             complement(8270..9412)
                     /locus_tag="PWZ48_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HDL2261864.1"
                     /translation="MKNIKKFFSRNIFPVFIKILPIQKNLIVFSSFGGRNYSGNPRVI
                     SEKIHEKKLNYRIIWLIKKEAIITDLPDYIKRIDSDSFLAAFYLYTAKYWIDDSRKTI
                     KYAKRKKQFYFQTWHGTPLKKIEFDAKDKLPKRYLSYAKKDSESITYLLSGNRYSSEK
                     YVSAFNIVPSKILEVGTPRNDELASSKEQVFDLKKKQINVLFAPTFRNNIEDNGITQL
                     EWLGVDNLREFFKKQQQELNLMTKFHPNVHSKLATDSKSIEYMKKHQITLINDGIPLE
                     MLFEEIDVLITDYSSIFFDFALTKKPIILFNYDEKEYSLERGFYIQLSELPIISVQDS
                     YGLVDVFKNQQEKLLKSSQELLDYIGNYETGASTEKVLELLERDIK"
     gene            complement(9416..10411)
                     /locus_tag="PWZ48_002221"
     CDS             complement(9416..10411)
                     /locus_tag="PWZ48_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2261865.1"
                     /translation="MHKISVIVPAYNVENYLENCLESISNQTHSNIEIIVVDDGSPDR
                     SGEIAEEYKKKDMRLKVIHQVNGGLSAARNSGLDSATGDFVCFIDSDDWIEPDYLELL
                     YFGTQTYGADISVLKIKKISSLEQLKEHTIAERKWEIFDREQAMKNLFINNKIGYSAN
                     NKLFNRNLFDNIRFPLGKLMEDKATTYLLIDHSEKVAVNSSAKYHYYQSPKSILRGSF
                     NPRIFDSFEVHEDILVFIDKFYPELSINVRTRYVYEAIRMMMTMIKNNYTNKEDYLRS
                     INILQRFEKEVKIDPDFSPKLKKLVKFLLINKNLPYYMSSSKIASKFLIKIESVR"
     gene            complement(10525..11514)
                     /locus_tag="PWZ48_002222"
     CDS             complement(10525..11514)
                     /locus_tag="PWZ48_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDL2261866.1"
                     /translation="MNQNLEKQTKLSIIIPFYNAKNTLRRAVDSVLTQGYPNIEILLI
                     DDGSTDGSSDIGDEIANLESKVACYHLENAGPSVARNYGIKEATGNLVGFLDSDDYFL
                     PDCFNNVLKDYHRNHPDIIAFGLSKGKNIENANLFVPEKKIGCSSEEAIEQMFRSKAV
                     DFYVWNKFFKRELFNHIVFPENKLYEDMVPMYEAFRAASKIDILPFSGIFYYQNNDSI
                     VYQSFNSKQYDNILQRKILVDEVKKDYPRLINLANARLVDGYLSTGFKITGKNQNQKE
                     KQKYYNQSKREIKSIYSKIRSNNEISIAKKLALFLYLINPKTYFILYKKVLKK"
     gene            complement(11486..12316)
                     /locus_tag="PWZ48_002223"
     CDS             complement(11486..12316)
                     /locus_tag="PWZ48_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2261867.1"
                     /translation="MENKNISILLSLYDKEKPEYLKEALASIFEQTVMPAEIVLVYDG
                     PINEKLQAVVTYFQQKVPHLFTVVKLEKNQGLGIALATGLEHTKNRLVARMDTDDIME
                     PHRLEKQLEIFQEYPEIAIVGSNIEEFTGTLSNVIGRRIVPEYNDEIRVFSKRRNPFN
                     HMTVMFDKNAILEVGNYRPLIGFEDYYLWVRLLKAGYKGYNIQEILVHARAGADMYAR
                     RGGSKYLLPGIKGRYRVWKDGLGSLKDFLIVICGHLVISLLPNKIRGKVYESKLRKTN
                     "
     gene            complement(12354..13745)
                     /locus_tag="PWZ48_002224"
     CDS             complement(12354..13745)
                     /locus_tag="PWZ48_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HDL2261868.1"
                     /translation="MNKNGEWNAARRIFIILMDVLVLNVSIYLSFLLKFQGEVPVRNL
                     ETFQHSAIFISIIFIALNILLGAYVFYNRMISDIVFITVIGQVLIALGIMVVTFAGRW
                     FAFPRGVILISFILGTILLSVYRIVVYKLYLRVSRDKKVLIVGLEKDVAPAIQNFQSK
                     KNNKHKVEAVAIDNYYENTKKLIDDMDIVYLASKIPEDEKINIYQLVTKKEKKLFLNT
                     TFENLTMINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLILLILASPFMLIT
                     AILVKTTSPGPIIYKQVRITKNQKEFSIYKFRSMSATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNEENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLLLDLKILLQTIKILFDKMSSQGLDEDENISS
                     LAESAFPKEKTYR"
BASE COUNT         4733 a         2537 c         2073 g         4639 t
ORIGIN      
        1 aagttctctt tttcatgtaa aatatttaat tatatatttc ctaaataaaa agtaaaacaa
       61 gaattaaata gttggttata caactttaat tcttgtttta ctttttagaa ttatttatcc
      121 aaagatgcaa tcattttttc atcaaatttc tgtctttaaa taccgttcca ttccgctcaa
      181 tatatcaaat ttaggctgat agcctaaaga acgaattttt gaaatatccg ctaaggaatc
      241 acgaatatcc cctgaacgct ccgcttcata tttaagcgct agctctgttc ctaagatttg
      301 atcaattgaa tggatcaatt caagtagcgt agtgcttttt cctgtaccga cattgaattg
      361 ctgtcctaaa gctttttctt ccttggcaac aagtaaaagt gcttgtataa catcttctat
      421 ataaacaaag tctctagact gactcccatc gccaaataaa gtaaattccg ctttttctcc
      481 tgccaattgt tttttgtaac gatccactag aatcgaaatg actccgctat atggagagtt
      541 tggattttga tttggaccat aaacgttaaa aaatcggaca gcactagtcg gaacatcata
      601 taaatgacag taatccaaaa catattgttc tgctgcaaat ttatcgatag catatggtgt
      661 taatggacgg atgactgatt cttctttttt tggcagtgtt ggttcatcac cataaactgc
      721 agctgatgaa ctaaatacta atcgttttaa attagattga tatttccgtg ctaactcaag
      781 caataaaagc gcactgtcaa aatttacacg atgtgtttct acaggccgtt ctacagaatc
      841 tgccacacta gctacagcag ctagatgaaa aatataggca aactgatatt ctttcatgat
      901 tctttccatc aattgagaat ctgatacatc cccctcaatg aaagtcactt gtttatccat
      961 atccaagttt tctttttttc ccattgataa atcgtcaact actacgattt tgttttcttt
     1021 tcccaaataa ttcgctagcg ttgaaccgat aaaaccagct ccgccagtaa tcaatatagt
     1081 gtccatcaaa ttcactccaa ttgcacattt tcgtatcaaa taatattagt ttaccatttt
     1141 tatattgtaa aaactataaa atttgttgaa gttgggataa ctagttttct tttctacttt
     1201 catataccta aaaatagttt caattagtta ctccttcttc tttagatata gttttttgga
     1261 tagaacccag atatccccaa aaaataaatg aacctatcgt actaaccaaa atgagttctg
     1321 tgataaataa tgccgaaact gctattgtaa gaatcattaa aatagcaaaa aagtgcttat
     1381 cttttactag atttggttgt gtgaaacaat aacgcaaggc tttaaagcat tggataaata
     1441 agaaggttaa caatgtcaat agacctaatc ctcctgtagc agccatcact aagaaaataa
     1501 aattatgagc agttaaagta tcttttccca ttaatgtata tggtagttta tcatgtgcat
     1561 attcaatata gtagcctgga gaacctccgg atatcggctt actcttgaat atttcaattg
     1621 cacttttcca aagttcaaac cttaaattag aaacatcccc gctatcagtg tcagggcgtt
     1681 ttaaatcaac tttattgctg ctagttgttc ctgatgaatc agatgtatct ttttttactt
     1741 ctgtaatatg cggaacatgt gcccatgcat gagcagttat atctgttaca taatacatcg
     1801 caattatact tagcaatcct actactaatg cagttagcca tctttttaaa gtattagttt
     1861 taagaaaacg actactataa cttgtaaaga aactaaaaac aaaagttgtt attaataaac
     1921 aaactaatgt ggtgcgtgaa cccgataaag caataaacga gaattgaata aaaatattaa
     1981 ttacaaaaaa tattttaaat agaagattta aatgcttgta agtgaccaaa taatatatag
     2041 caataatgat gacgattaat gatataaccg caccaaaatt tggatctgta tatacaccaa
     2101 ataaacggtt gcctaaccac cctaaacgaa ctgggtaata cttatatttt attggaattt
     2161 ttatgccata ttgaatgaca aaccagtata atgatactaa attagctaaa aaccatacca
     2221 atgaaagtat ccaggtaaat actttgaaaa aaaagttact actatagtct ttgccaaact
     2281 gataaatcat aaagaaaaag attaattgcc acatgattaa cttaatattt tcgctatatc
     2341 catattttaa attcattaaa ctgcttacta gcatcgcgcc aataaaagca attaatagcc
     2401 aatcatattt ttttagattt ttttctctaa atgcaataat tgcatctata atgatcaaga
     2461 caactcccgc tattccagca ggccaaaaaa gtaaagtatc atatctagta ggtaatagta
     2521 aatacgggcc tattgctcgt aacattaaaa taaaaccaaa ataaataaaa taccaacctt
     2581 tcacatattt taaaaatctt tcattcaaca tttttcctcc atccacctat ttctcataaa
     2641 tatttcttat cctttttagg ataaactggt cctttcaatt ctaacaaaaa aatgctaaac
     2701 taacaatgta tattaatgaa tttcgagagg tgaaagatga aaagagtaat tacttacggt
     2761 acctttgatt tgcttcatta cgggcatatc aatttactga aacgagcaaa acaatacgga
     2821 gactatctta ttgttgccct ttctacagat gaattcaatt ggaatgcgaa acaaaaaaaa
     2881 tgttatttta gttatgaaaa aagaaaacag ttacttgaag ctattcgcta cgttgattta
     2941 gttattcctg aagaaaattg ggaacaaaaa gtaaaagata taaaagaata ccatgcagat
     3001 tatttcgtga tgggcgatga ttgggctgga cattttgatg aacttgaaca actaacagat
     3061 gcacaagtga tttatttacc aagaacacct gagatctcta caactcaaat aaagaacgaa
     3121 cttaattctt taaatagtga taaataatat tttttaaaca ataaaagaac aatatatctg
     3181 gataagacgt aaaaaaatgg ctctataatt tctaggactg atagaatttt ctgtcggtcc
     3241 tagatttttt gtaaaaaaaa acagacataa aaaaattccg atagaataga gtcgtctaaa
     3301 acatcaatct gaaaggaagt ttttgatatc catttctcat tctatcaaag agctcttcac
     3361 tattaaagat aaaaatattg aaattaattc ttttcaagaa actttttata aggacaagaa
     3421 agccatcgta atcgaaggtc cattagttcg cacttttaga cattgtctat tttgtaaaga
     3481 atctgcaaag cagatcgaga aaaacggcaa gaagatttca atgattatct taaatcgcag
     3541 tgggaataaa caaacctatt tgcgtcttag aaaacaacat taccactgcc aatcatgcgg
     3601 gaaatatttt actgctcata cgtatcttgt cgctccttca tgttttattt cgaaacaagt
     3661 tcattacacg atattagaag agttgaccga gcgtcaagca ttaaagacaa ttgcgaaacg
     3721 ttgtgatttt cagtaacgac agttcaacgg acattatctg ctctatcggc aacgctgcga
     3781 cagaagctag actatttacc tcattgttta ctattcgacg agtttcgctc tctttcaatt
     3841 ttccatggga agttctcctt ttcttgtatg aattgagaga cagggaagct ctttgacatt
     3901 ttgtccagcc ggagaaaaaa aagatttgat tgcttatttc atgcgtttta caagaaaagc
     3961 tcatatgggg gttcgataca ttgtcacaga tatgaatgcc ccttattttt cattagtcaa
     4021 agaacccttc ccaaatgcac aaatcatcat tgattgcttt cacattgggc aacacttgaa
     4081 caagtttttc gatcgtgttc gaaaacgtgt gatgaagcag ttgaatcaaa aatgtcaacg
     4141 gcacctttaa aatgtaggaa aacggcaatt taaaattgtt ggtttaccta catttttgta
     4201 tgctcttctt atcccaaatg aaatggaggc tcagggatga gaaaagacat acgcgaagga
     4261 gtgatgatct acgtgatcaa tgaaatcaaa cccaattatg ctgcactagc aaaacaatac
     4321 gactgcgact accgaacagt taagcatgct tacgaagaag cccaagttaa agaatcaaaa
     4381 cctccagaac gtaagaagcg tccaagtaag ttagatccct acagagaaat catccaagac
     4441 aaaatcaaag accaatgcag tgcttacagc attttcaagt ttattgagca caaaggattt
     4501 ggaggaagct acagtttagt aaagacctat tgtcgccaga ttaagcaggc gaaagtacac
     4561 aaggcaaccg tccgagtcga acatagccct gggctttctg ctcaggttga ctggaaggaa
     4621 gatatgcgac taatcagtaa tcagggcgaa atttttgagt tcaatatctt tttgtacgtc
     4681 ttgccatatt ccaagaaaaa atatgtcaca ctaactttcg accgaaaaca agacacactc
     4741 tttgagtgct tagatgatgc gttttatcaa accggtggcg ttcccaaaga aatctggttc
     4801 gataatatga agacagtcgt tgaccattct cgtagtcagt tttccaaacc gcatttcaac
     4861 aaacgttttt atgctttcag taaggatgcc ggcttcacac caattgcctg tcggccattt
     4921 cgtccgcaaa caaagggctg tgtcgaagct ttagccagaa ctgttgaccg cctacgggtc
     4981 tataatcatg aattttatga tgccgttgag ctgtgtcatt tagtgaataa cctttgtgtt
     5041 gagttaaata acgaggtttc tcaatcaact gatcaaacac cagatgaacg ttggcagacg
     5101 aacgaaaaag agcacctgca tccataccaa aaaaaccttt tgaatcccta ttttgaagac
     5161 tttattcgcc gtaaagtcac caaggaagaa atggtcattt tccgtaaatg caggtactct
     5221 gtggcgcctc gctatatcgg taaagaagtc gaaatcgaac tctccgataa agaagaccaa
     5281 gtacaaatct attataacgg agaactgatt cggtcgcatc cattaacgac aaaacagctg
     5341 aactaccacg aggaagacta tttccaaatc ttgaaatccg atgtcatgag tcacaaagaa
     5401 gacgatggaa tcagagcgta tactcgtgaa agtctcaagc aatatgatct ggtggaggtt
     5461 gccgatgaag aatagccatt accaagaact gatgaccaac ttagagactc ttggtttgaa
     5521 tcatatgcgg gaatatgttc ctaactacat cgaagtggcc aatcgagagg agctttcttt
     5581 aacggaagct cttctcgagc tgacacgcag agaagttgat catcaaaata agcaaaaaat
     5641 gaatcgtgtg gtggcacgcg cccgcttttc caagagaacc agcctggaag agtttgattt
     5701 tgccttccag ccaagtatca ataagcgcga agtgctagac ttaaggcaca tgggctttgt
     5761 ggaaaaacat gagaacttgg tttttatcgg cagtcctggt gtaggcaaga cacatctgac
     5821 gattgcattg ggaatcgaag cctgtcgtca aggctatcgg acactattca tcacctgtca
     5881 tgagctgctt ttgcgtctgc gaacagccta tgaaaaaggt accctagagc gtgttctcaa
     5941 acgatacgct cgctatgacg tcttgattat tgatgagatt ggctatcttc cgattcaaaa
     6001 acaggaagct gatttattct ttcagcttct tgccatgcgc tatgaacacc gctctacgat
     6061 cattacgacg aacattgtct tatctcgttg gggggagttg ttccaaagtt ctgagatcgc
     6121 agctgccatc cttgaccgtt tagtacatca cgtcaaggtg ttcaagatca caggcaagtc
     6181 ttatcgtatg agagacaaga tctaaagtgt cgattgtcga caattactat tgccgaaaac
     6241 ctacattttt atcctgccca aaacctacaa ttttaagctg cccttgacat caaaaagaca
     6301 cttcacaagc taagtgctat cgacaattga aacctttgta taaattactt ctataatcag
     6361 atgacgagtt ggattataca acattcaaga aatggcagaa ttttcaatgg cgctatttaa
     6421 cagaaagtga agtagttgat tgtctaccct caatctctta tgaacttaaa atggcttagc
     6481 agtattatca aaagctgctt aacaattatc acaagaaaca gctagctact tcgagaaatg
     6541 accaaagaag ttccctcgaa acttcactat cttaaaaagc ctttttcata tctgaaaaag
     6601 gcttttaact agcattttcg aaaaagtatt ttaatacaaa attagagaat cttcgcacac
     6661 atatcaaaac attgaaacga tttgcttatg gattttgcag ttttaccaat atgcgcttgt
     6721 ggatttttct aatcaacggg ttaataagct atgcattaaa agctagtaaa tcttactaac
     6781 tttgttgatc ataaatgacc ttatcagccc gatttgacaa agaactactt ttttacaaca
     6841 ttattttatt ttcgaataat ctttcttact tcatttcgta tatctggaaa aagaaaataa
     6901 acaaacggaa gataaataat gaaaatcatt attgatttaa ttgccatatc taaaaatgaa
     6961 gaagagaata attgtggaag aaagtaatag gccgaagtag tcaaagtaag attgactata
     7021 aaaaataaga caacgttctt cccaaagaaa taggatattt ttatttcttt tcgtacaaaa
     7081 aatactctaa agaaaaagac taataattct actaccacga gtacgattgt agccgctaca
     7141 gcgccataag taggcacaaa agctacattt ccaataatac ttacaatcgc accaatgatc
     7201 attggataag catactcctt atctttttct atagcaattg caaattggtt agcaaaaact
     7261 ccaccaattg gactaacaac tataattaga caagaaataa gcatgtttat tgtcatcggt
     7321 ttgaaattct ccccaaaaaa ccatggaaca aattcttttg tattgataaa caccacacta
     7381 aaaagaatta atgcaataat aatcgtgtaa tcaaaagatt tttttgtgta aactaaaata
     7441 ttttctgatt ttccctctgc ctgtgcttta gatattttag gcaacataac aatagtaatt
     7501 gatgttaaga ctgccaatat aatacgagca attttttgag actgatcata ataagataat
     7561 gctgttggat ccacaaaata cccaactatc gttttgtcta aagttgtgta cacttgcata
     7621 aacaactgag gagtcaaaat aataaaagct ggtttccaca actgtttaaa aacattagag
     7681 ttaaagagtg gtttacctac ttttccgtgg atactcaacc agaaaactaa acttcctaaa
     7741 aagttagtaa ttgaagtaat gaacacataa atccacaaat catctttagt tgaaacaaaa
     7801 aggattatta acaataccga tgataatttg ataactgtat ttctaataac cactttcgaa
     7861 aattcttcaa ttccttgaaa aaaccaagaa atatctaatg ttagccctgc aataaatatc
     7921 aattgaatta agtataaatt tttatacttt acatcaaaaa aataaatata ggctatatag
     7981 agaaaggaaa cactcaagcc tattaaaaat tgaagaaagt aaatattcca gaattgtaaa
     8041 gatagtgctt ttttatcttt ccttgattta gcgatatttc gaatacgaaa aggattcatt
     8101 cccacagcaa tgacagtcat taaaaattgt gatattgaga aaacataccc ataaatccct
     8161 aaaccagttg aacctattct acgactaagt ataggtatag taagtaaggg caaaacaatc
     8221 aaaaagactt gatatatcgc attataaaat atatctttta ctgttttttt cactttatat
     8281 ccctctctaa caactcaaga accttttctg tacttgcccc tgtttcataa tttccaatat
     8341 aatcaagtaa ctcttgagaa cttttcaaaa gcttttcttg ttggttttta aaaacatcaa
     8401 caaggccata actatcttgt acgcttataa ttggtaactc acttaactgt atataaaaac
     8461 ctctttctaa gctatattct ttttcatcat agttaaaaag aataattggt ttttttgtta
     8521 gcgcaaaatc gaaaaaaata gatgaataat ctgtgattaa aacatctatc tcttcaaaaa
     8581 gcatttcaag aggaatccca tcattaatta aagtaatttg atgttttttc atatattcta
     8641 tacttttaga atcagttgct aatttactat gaacattagg atgaaattta gtcattaaat
     8701 ttaattcttg ttgttgcttt ttaaaaaatt cacgtaagtt atccacacct aaccattcta
     8761 gttgcgtaat tccgttgtcc tcaatattat ttcgaaaagt tggagcaaat aagacattga
     8821 tttgtttctt tttcaagtca aacacttgct ctttactaga ggctaattca tcgtttctag
     8881 gagttcctac ttctaatatt ttagaaggaa cgatattgaa tgctgaaaca tacttctcag
     8941 aactatagcg attaccagat aataaatatg taattgactc actatctttt ttcgcatagc
     9001 ttaggtaacg tttaggtaac ttatcttttg catcaaattc aatttttttt aaaggcgtac
     9061 cgtgccatgt ttgaaaataa aattgttttt tcctttttgc atacttaatc gttttccgac
     9121 tatcgtctat ccaatactta gctgtataca aataaaaggc agctaaaaaa gaatcggaat
     9181 caattctttt aatataatct ggtaaatcag ttataatcgc ttcttttttg attaaccaaa
     9241 taattctgta attcaatttt ttttcatgaa ttttctctga aataactctt ggatttccag
     9301 aataattacg cccgccaaaa cttgaaaata caattaaatt tttttgaata ggtaatattt
     9361 taataaaaac cgggaaaata ttccttgaaa aaaatttttt tatattcttc atctcttacc
     9421 tcacagattc tatcttaatc aaaaatttac ttgctatctt actacttgac atatagtaag
     9481 gcaagttttt atttattaat aaaaacttta ctaatttttt taattttggt gaaaagtcgg
     9541 gatcaatttt cacttctttt tcaaagcgtt gtaaaatatt tatagaacgt aaataatctt
     9601 ctttattggt atagttattt ttaatcattg tcatcatcat ccttattgct tcatagacat
     9661 agcgagttct aacattaatt gataattccg gataaaactt atctatgaat actagtatat
     9721 cttcatggac ttcaaaagaa tcaaaaattc taggattgaa gcttcctctc agtatacttt
     9781 tagggctttg gtaatagtga tatttagcag atgaattgac agcaactttt tcggaatgat
     9841 cgatcaacaa ataagtagta gccttatctt ccattaattt acccagtggg aatcttatat
     9901 tatcaaataa atttctattg aataatttat tatttgcaga atagccaatt ttattattta
     9961 taaataagtt tttcattgct tgttctctat caaatatttc ccattttctc tcagcaattg
    10021 tatgttcttt tagttgttct aaacttgaaa tcttttttat ttttagcacc gagatatctg
    10081 ctccataagt ttgcgtacca aaatatagta actcaagata gtctggttct atccaatcat
    10141 cgctgtcaat aaagcacaca aaatctcctg ttgcagaatc aagaccagaa ttcctagccg
    10201 cacttaaacc accattcact tgatgtatca cttttaatcg catatctttc tttttatatt
    10261 cttctgcaat ttccccactt cgatccgggg aaccatcatc gactactatt atttcaatat
    10321 ttgaatgtgt ctgattggaa atagattcta agcagttttc taaatagttt tctacgttat
    10381 atgcgggtac tataacacta attttatgca ctctcttcac ttcctaaaaa tcatcttttt
    10441 tatttttaca aatgtatcat taccagtaac aattttagtc ttccgaagtt aataaacata
    10501 aaaaataaca tatcgaaaaa aactttattt ttttagaact tttttatata aaataaaata
    10561 agttttagga ttaattaagt ataaaaataa agctaatttt ttagcaattg atatctcatt
    10621 gttacttctt atttttgaat atatactttt tatttctctt ttagactgat tataatattt
    10681 ttgtttttct ttctgatttt gatttttgcc agtaatttta aaccctgttg aaagatatcc
    10741 gtctactaac cgagcattgg ccaaattaat aagtcgagga taatctttct ttacttcatc
    10801 aactaatatt tttctctgca aaatattatc atactgtttt gaattaaaag actgataaac
    10861 gatgctatcg ttattttgat aataaaaaat acctgaaaat ggcaaaatat caattttact
    10921 agccgcccta aaagcttcat acataggaac catatcctca tataatttat tttctggaaa
    10981 aactatatga ttgaataatt cccttttaaa aaatttattc catacgtaaa agtccactgc
    11041 cttacttcta aacatttgct caattgcttc ttcacttgaa caaccaatct tcttttcggg
    11101 aacaaataaa tttgcatttt ctatattttt tcctttactt aatccaaaag caatgatatc
    11161 ggggtgattc ctatggtagt ctttcaaaac attattaaaa caatcaggta aaaaataatc
    11221 gtcactatct aaaaatccta ccaaatttcc agttgcttct tttatcccat agttcctagc
    11281 aactgatgga ccagcgtttt ccaagtgata acatgctact ttactctcta gattagcaat
    11341 ttcgtcacca atgtcactac taccatctgt agatccatca tcaattaaca agatttcaat
    11401 attgggataa ccttgcgtta agacgctgtc tacagctctt cttaaagtat tctttgcatt
    11461 gtaaaaaggt atgattatag aaagtttagt ttgtttttct aagttttgat tcatatactt
    11521 ttcccctaat tttattcggt aataaactaa ttacaagatg tccgcaaata actatcagaa
    11581 aatcttttag tgatcctaga ccatctttcc atacgcgata tcggccttta attcctggta
    11641 aaagatattt acttcctcca cgtctagcat acatgtcagc acctgctcta gcatgtacta
    11701 gaatctcttg gatattatat cctttatatc ccgccttcaa tagtcttacc cacaaataat
    11761 aatcttcaaa tccaattaat gggcgatagt ttcccacttc taaaatagca tttttatcaa
    11821 acatgactgt catatgatta aaagggtttc ttcttttcga aaacacacgg atttcatcat
    11881 tatactcagg tacaatcctt cttccgataa cattgctaag tgtgccagta aattcttcaa
    11941 tattactccc aacaatcgct atttctggat attcttggaa gatctctaat tgtttttcca
    12001 atctgtgtgg ttccatgatg tcatctgtat ccattctggc cactaagcga tttttagtat
    12061 gttctaatcc agttgctaaa gcaatcccca atccttgatt tttttctaat tttacaactg
    12121 taaacaagtg aggaactttt tgctggaaat aagtgacaac tgcctgaagc ttctcattta
    12181 tagggccatc ataaaccaat acgatttctg ccggcatcac cgtttgctca aagatactag
    12241 ctaatgcttc ctttaaatat tccggctttt ctttatcata caaagaaagc agtatactta
    12301 tatttttatt ttccaatgtt ctctcctact tctttgctca aaatttttcc aatctaacga
    12361 tacgtttttt cttttggaaa tgcactttct gctaatgaac tgatattttc atcttcatct
    12421 agtccttgtg aggacatctt atcaaataag attttgattg tttgaagtaa aatcttcaaa
    12481 tctaatagta aggaatactt tttgatatac aacaaatcaa aacgtagttt actgttataa
    12541 tctgatacat attttccgta tacttgtgca tatccggtaa ttcccgcacg tacattgtgt
    12601 cttaagtaat aataaggatt ttcttcattg aactgatcaa cgaaaaatgg tctttctggc
    12661 cttggaccga caatcgacat atcgcctttc aatacattaa agatttgagg taattcgtca
    12721 aaacgtacag cacggataaa tttacctact ggtgtcacac gtgcatcgtt acttttagcc
    12781 aagactggtc ctgattttgc ttcagctgtc gcactcatgg aacggaattt atagattgaa
    12841 aattcctttt gatttttcgt aatacgtact tgtttataga tgattggtcc aggtgaagtt
    12901 gtttttacca aaatcgctgt gattagcata aaaggtgagg cgaggatcag taaaataagc
    12961 gaaacgatga tatcaaagag acgcttgaat agttcgtatt ctggttggat tctgaaccca
    13021 gaagcttcaa tgatgctttc atcttcaaaa ttcataatat tcggatttat cattgtcaga
    13081 ttctcaaaag tcgtatttaa aaataatttc ttctcttttt tagtcactaa ttgataaatg
    13141 ttgatttttt catcttctgg gattttagaa gccaaataga cgatatccat atcatcgatc
    13201 aatttttttg tattttcgta ataattatcg attgccactg cttcaacctt atgtttattg
    13261 tttttcttag attgaaaatt ttggatagct ggtgcgacat ctttctccaa acccacgata
    13321 aggactttct tatcacgact gactcttaag tacagtttgt aaacgactat ccggtaaaca
    13381 ctaagtaata tcgtacctag gataaacgag atcaaaatga cgccacgcgg aaatgcaaac
    13441 catcggccag caaaagtaac taccatgatc cccaatgcta ttagtacttg tccaataaca
    13501 gtaatgaaga caatatcact gatcattcgg ttatagaata cataagctcc taacaaaata
    13561 ttcaacgcaa taaagatgat ggaaataaaa attgctgaat gctgaaaggt ttctaagttt
    13621 ctcactggta cttctccctg gaatttcagc aaaaaactaa gatagatcga tacatttaat
    13681 acaagtacat ccataagaat aatgaatatt ctgcgtgccg cattccactc cccattttta
    13741 ttcatatata cactcctaac cataaaataa tgcctctatc atcttaccat agcaaagttt
    13801 aaaaacctag tgaataacca aattgtaaag gaaattaaaa gaaacgggga aaagcgttta
    13861 ttgtctgttt atatgtgtat ttctgcataa cagaaagttg tctcagaagt cccatcactt
    13921 cctttatgct gaactagtat tctcctgcta attccttatt cacaagttac aatattcata
    13981 ta
//
LOCUS       DANKEF010000062        13819 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.062, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000062 DANKEF010000000
VERSION     DANKEF010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13819
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.062"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..224)
                     /locus_tag="PWZ48_002225"
     CDS             complement(<1..224)
                     /locus_tag="PWZ48_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261869.1"
                     /translation="MTIELEEHLLWFKYDPSEKFVKDFYKVWDSEVFFLAIEDSLLIN
                     LYYSNKNYFKIPAAKTRMKKGVYFLFDIVT"
     gene            complement(751..823)
                     /locus_tag="PWZ48_002226"
     tRNA            complement(751..823)
                     /locus_tag="PWZ48_002226"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(788..790),aa:Lys,seq:ctt)
     gene            1204..1773
                     /locus_tag="PWZ48_002227"
     CDS             1204..1773
                     /locus_tag="PWZ48_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HDL2261870.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1760..2620
                     /locus_tag="PWZ48_002228"
     CDS             1760..2620
                     /locus_tag="PWZ48_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2261871.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2810..3514
                     /locus_tag="PWZ48_002229"
     CDS             2810..3514
                     /locus_tag="PWZ48_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2261872.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            3519..5354
                     /gene="walK"
                     /locus_tag="PWZ48_002230"
     CDS             3519..5354
                     /gene="walK"
                     /locus_tag="PWZ48_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HDL2261873.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            5351..6667
                     /locus_tag="PWZ48_002231"
     CDS             5351..6667
                     /locus_tag="PWZ48_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261874.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            6668..7537
                     /locus_tag="PWZ48_002232"
     CDS             6668..7537
                     /locus_tag="PWZ48_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HDL2261875.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            7592..8401
                     /locus_tag="PWZ48_002233"
     CDS             7592..8401
                     /locus_tag="PWZ48_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HDL2261876.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(8504..9133)
                     /locus_tag="PWZ48_002234"
     CDS             complement(8504..9133)
                     /locus_tag="PWZ48_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDL2261877.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            9303..10595
                     /locus_tag="PWZ48_002235"
     CDS             9303..10595
                     /locus_tag="PWZ48_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2261878.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(10671..11333)
                     /locus_tag="PWZ48_002236"
     CDS             complement(10671..11333)
                     /locus_tag="PWZ48_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HDL2261879.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            11558..11842
                     /gene="groES"
                     /locus_tag="PWZ48_002237"
     CDS             11558..11842
                     /gene="groES"
                     /locus_tag="PWZ48_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDL2261880.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11893..13518
                     /gene="groL"
                     /locus_tag="PWZ48_002238"
     CDS             11893..13518
                     /gene="groL"
                     /locus_tag="PWZ48_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDL2261881.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4631 a         2367 c         2936 g         3885 t
ORIGIN      
        1 tcagtcacga tatcaaacaa aaagtataca cccttcttca ttctagtttt cgcagcagga
       61 attttgaaat agttcttatt agaatagtag agattgatta ataagctatc ttcgattgct
      121 aaaaagaaaa cttctgaatc ccacacctta taaaaatctt tgacaaattt ttctgatgga
      181 tcgtatttaa accaaagcag atgttcctct agctcgattg tcatgttttt cacctcaacg
      241 aaagtatacg aactaatgtt tgttttgtaa aggataaaaa tagggtagca accggtaccc
      301 aaactagtac ccatttatca aaaacgacga aatttatgta aatagaaaaa taagaataaa
      361 cattgatgta tcaacattta tatatcttta gaacaaatga aaagggtggt tctcataaga
      421 aaaacatgtt cgcacttcta ggcaaaccag gatttgaaga ccttgctaaa gaattgaacg
      481 aacgcctgta aaataaggat ttgtcgctag atttaaaaaa tcagtgatag aaacatgttt
      541 gctttaaaat gaaactagtg atagttttag tgatagtgaa cttaaaaaaa gcactcaatt
      601 tgagtgcttt tttattgtta ttaaaaatta aaaaactaaa atacataccc gaatttcata
      661 cccgaaatat aaaaaatgtc atcacgtaca tctttttcga aactaccgaa gtagccaaag
      721 atgtactgat gacgtgggtt tggcacccaa tgggccgtga ggggctcgaa cccgcgaccc
      781 gctgattaag agtcagctgc tctaccaact gagctaacgg cccgtacaaa aagtacttaa
      841 taatagtagc actaagaatt ttaaaatgca agactacatt taaagaaaat cagaaaaatc
      901 tttacttcct tataggaaat cattcataga ttattcacat ttgctcgcta ttatataagc
      961 ataccaacaa acaaagttct tgcaagagaa caaaccctaa cacttttttc attttttact
     1021 cctttcccgc cgtctccccg gcgggtctcc tgttcttaac gataaaagac aagaaggctt
     1081 agccttcttg ttttttgttt tcatggaatc ccgctaatca aatcacctag ttaaagcaac
     1141 tagattgaat gtttatcata agtctgatat catgtttatg aaatgaaaaa aggagcggtt
     1201 atgatggaaa aaatgcgtca gcaattaatg gagtggggga aggaactaga aacattccgt
     1261 ctgcctcact gggaagaact ccctgactta gaactttata tggaccaagt cagaacgtta
     1321 gttgatcgtt atctttcacc tgtgatacaa ggagaaaagc atccattgct tacctcttct
     1381 atggtaaaca attacgttaa actaggtttg ataccagcac cagttaaaaa aagatataac
     1441 aaagaacacg tagcattttt acttgcaatc actacgttga aacaagtatt gactattcct
     1501 gaaatcaaag aagggatatt gttccaagga aaaacagtag gaattcgtga agcctataat
     1561 ctattttgcg atgaacaaga agcagctgtg tggatggtca gccaattagc acaaggtaaa
     1621 tctcatccgc aaaaattcga ccaggcaacc cctgtagaat atattgctgt aagagcggca
     1681 actttatcgt ttgcaatgaa acttcttgcg gaaaaaacga ttgttctaga aaccgaatat
     1741 ttaaaagagg agaaaacaaa tgaaaaacaa taaaatcgca attttagtag attcaggtac
     1801 ggatgtgcca gaagaattga taaaaaaata cgagatgtat atgatcccat tgaaaattat
     1861 ttataaagat caggtatata cagataaaat tgacattacc tctgaagaga tctatcaacg
     1921 tttgccagag gaaatccctg gtacatcttt gccggatggt gagtcgatca cagaaatctt
     1981 tgagcaaatc aaaaaagacg gttatgaaaa ggtacttgcg gtaacaattt ccagtggatt
     2041 gagcgggaca tataatgtgg ttcgtttaat tgcacaacag caagaagaat tggaagcata
     2101 tgtactagat acgaaaaata ttggtattgg tgcaggtttt tctgcgatcc aagcagcaaa
     2161 atggctggaa gaaggagtag aatgggatca actgatcagt aatctgaatg agttagtcga
     2221 aagaacaaaa gttttcttta atgttgcgac attggaatat cttcaaaaag gtggacggat
     2281 cggtttagtt gcttctattg taggaaccgc tttaaagctt aacccaatca tctcatgtaa
     2341 tgaagaaggg atttattaca cagtaggaaa agctagggga cgtaaaaaaa gtttggataa
     2401 aacggttgct tacgtcaaag aaaaaatcgg taatgcaaag gtcttcaact tagctgttgc
     2461 ccatggggat gcgaaagaag aagcaattga aatggaagcg agacttaaaa gagagttccc
     2521 acaagcaaac caaatctact ttggccagat ttctccggca ttagtagtcc acactggtcc
     2581 gggattattg ggtgtcggtg tacaagtttt agaaggataa atcaaaaaaa ggaaacactg
     2641 aggtgaaaat cagtgttttc cttttttatt gaagttagaa caaaaaataa taaagtattt
     2701 tgcactttca ttttaatttt ctcttttgtt ttataggcaa agatgaaaca aaagcagaaa
     2761 tacggtataa tgagaaggta tgacaataat atataagagg tggctcttta tgaaaaaagt
     2821 tttagttgtc gatgatgaga agccgatttc ggatatcgtc aaattcaatt tagccaaaga
     2881 aggctatgat gtttatactg catatgatgg ggaagaagcc ttggaaaaag ttgctgaggt
     2941 agaacctgat ttgattttac ttgatttgat gctacctaaa atggacggct tagaagttgc
     3001 tcgtgaggta cgcaaaactt atgatatgcc gatcattatg gtaacagcca aagattctga
     3061 aattgataaa gtcctaggct tagagttagg cgccgatgat tacgtgacca agccattttc
     3121 caaccgagaa ttagttgcac gtgtcaaagc aaatcttcgt cgcggtgcca cggctgcgaa
     3181 ggaaccagaa gaggcagcac ctgcggaatt gacgattggt gatttgacga tccatccaga
     3241 agcttacatg gtgacgaaac gaggagaaac aatcgagtta acacatcgtg aattcgaact
     3301 actcttttac ttggcgaagc accttggaca agtgatgaca agagaacatt tattacaaac
     3361 ggtttggggt tatgattatt ttggagatgt acgaacagtg gatgtaactg tacgtcgttt
     3421 gcgtgaaaag atcgaagata atccaagcca tccaaattat ttagtgacac gtcgtggtgt
     3481 cggttactat ttgcgtaacc cagaacagga gtaattggat gaaaagaaaa gtccgctttt
     3541 ttcgatcagt caattttaag atcgctatta cctttattct gattttattg atctcgatcg
     3601 aaattattgg tgcctacttt atacgtggtt tagaacgttc cacgatcaat acatttatta
     3661 aagatatgaa tcaaacggtg gaatcattag caacaacaat cagcccggaa ttgaatcgaa
     3721 aagataatgc agatgatgaa gaggtaaatg cgaatatcaa aagatttatt gaaaacagtg
     3781 cgactagcga tatcatcgaa atacgggtcg ttgacgaaaa agggatcatc cgaggaacaa
     3841 ccgatgtcaa tgaacaaagc gctgtgggaa agaaaaatga ttatgttgat atcaatgatt
     3901 tcacgactaa acgttatgtt gctttagata acgacgataa acgggtgaat atcaatgttc
     3961 aaccgatcct gtccccgacc ggcgatgccg ttatcggggc tctttacgtc aaaagtaata
     4021 tcgaacaaaa atattcggaa atcaataaca cagcagtcat tttctttact gcctcactaa
     4081 ttgcggcatt tatttcaatg atcgtatctg ttttagttgc tcgttcgatt acacagccga
     4141 tcggtgaaat gagagaacaa gccattcgaa tcgctagagg agactacagt cggaaagtca
     4201 aagtgcatgg acaagatgaa cttggacagc tggctgatac cttcaatcag ttagctgaac
     4261 gtattgaaga agctcaagat acaatggaag ctgaacgcaa tcgtttggat agcgtgctat
     4321 cccatatgac agacggcgtc attgcgacag atcgcagggg taaagtcatt acgatcaatg
     4381 atatggctgt ttccttgcta gatatcaaaa acgaggaagc aatcggacaa tcgattttga
     4441 cacttttaga tatcgaagaa gaatatacgt tgcggaaatt attagaagat ccaaacgaaa
     4501 tggtacttga ccgttcaacc agtacattgg aaagtgatca gatgatcttg cgagtagact
     4561 tttctatgat ccgtcgcgaa tcaggtttta tcagcggatt agtagctgtc ttgcatgatg
     4621 tgacagaaca agaaaaaaca gaacgggaac gtcgtgaatt cgtctctaat gtttcccatg
     4681 aacttcgtac accgttgaca agtatgcgta gttatatcga agcattgagc gaaggtgcgt
     4741 ggaaagacga ggaagtagca ccaaattttt tgaaggtaac gctagatgaa acagaccgaa
     4801 tgattcgaat gatcaatgac ctcttaaatc tatctcgcat ggatacaggt aatacccagc
     4861 ttcaattaga atatgtcaat ttcaacgagt tagtgaattt tgttcttgac cggtttgata
     4921 tgatggtggg aaatcaggaa aaaaattata agatccgtcg tgaatttacg caaagagatc
     4981 tttgggtcga agtagataca gataaaatca tccaagtagt cgataatatc atgaataacg
     5041 cgatcaagta ttcaccagat gggggaacga ttacttgtcg cttattagaa acgcataata
     5101 acgtggtttt aagtatcacc gatcaaggat tggggattcc taaaaaagat ctgaaccgag
     5161 tgttcgagcg gttctatcga gtagataaag cacgtgcaag agcacaaggt gggacaggac
     5221 ttggattagc tatttctaga gaagtgatta aggcgcatcg aggcgctatt tgggcagaga
     5281 gcaaggaagg aaaagggtcg actttttata tctcgctgcc atatgaacct tatgaggagg
     5341 aatggtggga atgagaatct ctgaaaaagt cgttcgtata ggactcattt tgatggttgc
     5401 gttaagcata tacttctctt atgcaatctg gctgagccca gcaggaaaaa gttcaatcaa
     5461 cgtagatgag acaaacactc aaatgatcga gtctcaaagc aatcgaaaaa cttccgaagt
     5521 ctttcttccg cttcatgtta catggcacca ttcgggaatg atccaagaaa ccaacagtga
     5581 aaatctcgtg taccgattgc aattgatcat cgataaagca agatttggaa agctttctga
     5641 agtagtttca ggagacaaat cacagtttga acaggtgaaa aatctgaact ctggttttga
     5701 gttggcctat aatgcgcctt ttttgctaag agaatacaag gaagcatttg atttagattt
     5761 agatgtgtcg aacttggcaa aagaaagcga aaatatgtat tttactagaa tacaattcga
     5821 tcaaggaaat aaaaaaatcc gatttttgaa ttattcgaac catctcgtat atgaagcaaa
     5881 tatttcgttg aattggacag atatcaataa agaattaaca gcaaaagaca caaaatggac
     5941 agaaatgaca acagaaccat cgacgatcga cacgcaatat gctacgaaac attcgatcaa
     6001 actgaaaaag tatagttata tcctgtcaca gcaaccctat acacttttta gaaatgcttt
     6061 tttccagaaa ccagatagcg tgaaaaataa tgatgaaagc aatgaattga ttttttacgg
     6121 tgggaatgaa acaatgtcta tccattcgga aacgcaagta gttgattttc gtggagagct
     6181 gccagagaaa aaagaaaatg ctagtgtatt ttctgaaagt ttcccctata ttagcaaatt
     6241 aggcagttct ttagggaacc tcagatattt tgaccgcagt gacaatacga tcgattatcg
     6301 gatattcgtg gaaggcttcc cggtttttgg gacagacagc agaggacaga ttggtgtgga
     6361 agttggcggt gaaacgaatg gttctgtgca agtgaatatc caaaccagtt tgaatacgat
     6421 tcaagtgccg ataccttcag acgaagaagt agaacttgca agtacgaatg aaatcatcca
     6481 gcagttaaaa gctaagggag cagatagcaa caagattggt tcagtgatcg ttggctatac
     6541 atggcaaaat gtcaaagaaa cgaatcgtgt cgttgatctc ttacctgaat ggtatgtgaa
     6601 atataaagat aactggtatt cagctaacga ccttctggaa aaattacctg gattggaggc
     6661 gaattaattg gatttcaaaa aaatcgaatg gatatttttc gtcgcttttt tagggttgaa
     6721 tgtgttttta ttcagcagtt atcatgacgc ggtataccaa gaaaacaatg tcattcgctc
     6781 taatcagact gaatcaatcc aacaacgtct ggctagcgac gatatcactt acacggggaa
     6841 gatttcgtct gagaacaagc aaggctatta tcttagcgcg gagcagacca atcttagcac
     6901 ttcattagcg aattatcgca aggctggtaa aagcggcttg ctggcaagtg gtgtgttttt
     6961 agacggtgat gttttgacga aatctttatc cagttcagat gctgaaaaga acgtgatcga
     7021 tccagataaa attagtagtt ccttggaaga ttttttatct aacaaagaca atattttatt
     7081 cggtaatgat tatgtgtaca cgaaaaactt ttctcattta gaagaagaac cactagaaat
     7141 ccaagcaagc caagagtatg aaggcatccc attcaatgat gatacagcaa aaatcgttct
     7201 ttctttagaa aaaaacgata atgattatag catcacgaaa tacatgcaga cgcatttatc
     7261 tgatatcgag cagctgcgcg aaaagacgga actgtatacg gaagaggaag caatcaatac
     7321 attgtacatc aataacaaaa taccaagagg atcaaaaatt ttatggcgtc agctagctta
     7381 ttcctgtatt ctgaaagtcc gggaaaaaaa tgtgtatgtt cctgtttggc atgtagcgat
     7441 cgaaacaagc gataaagtgg tccaaattga gagtgtcaac gctttcagca atacgatcgt
     7501 gacaaacaat actgtaccaa aagtggaaga tcaataaaaa agcgagtata atgaaaaaag
     7561 tttacaaagt aagaagggaa gtaggagtca aatggctgaa gaaaaaccag cttttaaaat
     7621 cagtatccta gcaagtggaa gttcgggtaa ttcactttat atcgaaagtg aaaaaaaacg
     7681 gcttttggtc gatgcaggac ttagcggtaa aaaaatcact tccttgatgg ctcaaattga
     7741 tcggacaccg gaccaattag atggaatact tgtgacgcat gaacatcgag atcatataca
     7801 tggcgtgggt gtcttggctc gtaagtatca tttggatgtt tttgccaatc agcagacttg
     7861 ggaagcgatg gaaccactga ttggaaatgt accagtggaa caaaaacatc tatttgagat
     7921 ggggaaagtc agaacattcg gcgatatcga tattgagagt ttcggcgtct cccatgacgc
     7981 agcggctccg caattttatc gtttccataa agaaaatcgg tcattcgtca tgttgacgga
     8041 caccggatat tgcagtgatt atatgcgagg tgtcattgaa aatgccgatg gttatttgat
     8101 ggaaagtaat catgatcttg agatgttacg catgggacct tatccgtgga atttaaaaca
     8161 gcgtatttta ggagatagag gacatctttc caacgaagac ggtgcacttg tcatgactga
     8221 tgtgatcggt aatcggacca aacgaatcta tcttggccat cttagcaagg aaaacaatat
     8281 gaaagaactt gctcatttaa caatggaaaa tgttttgaaa gaaaaagatt ttggcgttgg
     8341 acatgatttt gaaatctatg atacagatcc agatgaagca actgagctat tttcgattta
     8401 aacagttcaa tcaataaaaa tataaaaagc agaagataaa atcaatcagg gttggtttta
     8461 tcttctattt ttattttttt agcatattaa aaaaataacc gtattatcga ctgactacac
     8521 tgatgtgctg cttttcatgt ttctcatcca gtatctcatc tactaatgca atcgcgtaat
     8581 ctgccatgct gatcgtactg tcgccagcag cgtttctttg taaataatca tcacttaaca
     8641 aataagtacc tgtacgtttg ccatgtggat caaataaaga agctggactg atataagtcc
     8701 aatgtagatt tttagattgt ttcaatttta acaatccttc gtacatattg aaagctgttg
     8761 gatagaaggg tgcattttta ggtacttggt caatcatacg agtcgtcttt tctgtatcta
     8821 agaataacga agaggcaccc ccaacgacaa ttaagcgtgt atcggttcct tcaagcatct
     8881 caatcaaatg ggacaaagaa gtttgatgca tttcttcttt tcctttggga gcgttgaaag
     8941 catcaacgac aacatcaaaa gatgcaagat cagtagttgt caactcaaat aaatcctttt
     9001 gtataaacgg aacatctgat ggcaattttt ctggatgacg cacgattgcc gtcacgtcta
     9061 aatctctgga caatgcttcg tctaaaataa atgaacctgc atggccagta gctccaatga
     9121 gtgcaatttt cataaaaaac ctcctttttt attcctgtcc attgtaagca gttgctgaat
     9181 attcgtcaaa gaatccgatt tttgaaaagc tcagtgcgca ttcctttcat ggttcgtgat
     9241 cattgggtat agttaaatta agaaaactga ttgataaaaa tagaagttga aggagataag
     9301 aaatgaaaga gtggttagat cgtgcctttt taccaatgat aacatctgtt gtgaatctta
     9361 gacccttaac tgctttgcgt aatggaatga cttacacgat cccaataatt attgttggct
     9421 cgctgttttt cttatttgct cattttcctg tggattacat cgctcagtgg atagacaaaa
     9481 caggattttc tatgtatttt acacaagtat accaggctac atttgatgtg atagcctttt
     9541 ggatcgtttt tgccattgca tacgcttatg caaaagaaga agacatcgat cgcttaccag
     9601 tcggcataac agccttgtct gcttatctac tgcttattca gccactaaat actcagatag
     9661 atactgtctg gacaggtgct gaagggatac taggagcagt tacaactggg ctcatcatcg
     9721 gatggggcta tgcacgttta gtaagacttg cgaaagggtg gaagcaaaga gagaagatac
     9781 ctgaaaatgt ttggcagtct tttacttcat tgattccaat cattgttata gtcactttta
     9841 cgcttatcgg ttcagcggtt tttcaacagt tgttcagaat gactgttgtt caaggagtat
     9901 ggacatatgt ccagcttccc attcaatcgt tcatagacac gttgtttggt gtgcttttat
     9961 taggtttttt tgttccgttt ttatggttat ttggtcttca tggatctgcg atggtcaatg
    10021 ggttggttag tcccgtcttg caagcgaatt cattagcgaa tgcagaaatc ttagcctcag
    10081 gacaagagtt gacggtggca aatggcggac atatcgttac tcagcaattt ctcgatcagt
    10141 tcatgacggt tacgggtgct ggattgacgt taggggctgt attttttatg acggtttttg
    10201 cgaaatcgaa gaaatacaga gaattaggaa atcttgctct tttaccagcc tttttcaaca
    10261 tcaatgaatc aattattttt tccacaccga tcgtgatgaa cccgatgatg gctgttcctt
    10321 ttattttcgc accggtcgtt tcaggattga ttacctattc cgctttatat tttggattcg
    10381 ttcctttatt tacagcagtc caagtccctt ggacaacacc gccgatcctc tcgggtttct
    10441 tagtaggcgg agtgcctgca gcgattttac aaggtatcgt gttgtcaatt agttttttca
    10501 tttatttccc atttttgaaa aaaatagatt ctgcaaactg caaaaaggaa agacagacaa
    10561 aaaatgatgg agcagcagaa aagatgaacg actaaaaaac aaaaatactc ctgtcgcttt
    10621 agattgggaa aatcaccaat ctaggatgac aggagtattt atagtcatgt ttatactagc
    10681 cctttttgga cagctaattg aacaagaaca actaatgtgt tcattcctac gtgtgtgatc
    10741 atcgatgtcc agatccgacc agttgctttg tactctaggc tgaagaagaa acctaggaaa
    10801 aagtagatta gcagatgacc atcattatga gcaaacgcaa acagcaaaga gctgatcact
    10861 gctccgaccc aaaaatttga atatcgactg atgatcccca gtatactgcg gcggaatacg
    10921 aactcttcca tgatgggtcc agcaacagtt gtcgccaaaa taaaagcggg tgcttccatg
    10981 atcatctgga tgatattttg tgtattctcg gaaggggttg ctttgccaaa caatacagat
    11041 tctaacatga cagcgatgct ttgcagaatc aatgcgacaa agattccaac caatccataa
    11101 ataacaatct ttggagcaga agcttttttt accgctgatt cgatcgctaa gggttcacgt
    11161 tgcttaaaat aaaaaatagc taggacaacg gcgcctagta tatacatgac tgcagttgct
    11221 gtgatcaatt cagacgtaga ttttacaagt cctatcgaag ctaggatcaa gggtgagata
    11281 aaaattagac catagcaaaa aatgctgagg aagctatatt tttttaaaga catgtaattc
    11341 ctccttttaa gggataacaa gcatgcaagg caagcttata tcgattgatt tcaattatat
    11401 cataatttca ataagcagct gtcagaataa aatttctttt ctttgattta aaagcttgca
    11461 aaaaagaggg agaatgagta ttattaaaaa tgtaggttag cactcgaagg ttaagagtgc
    11521 taacagatta aaaaaagagt tatcggaggg atttatcgtg ttaaaaccat taggtgatcg
    11581 cgtcattatc gaagtcgcaa aagaagaaga aaaaactgtc ggaggaatcg ttttagcttc
    11641 tgctgctaaa gagaaacccc aaactgggac agttgttgca gtcggtgaag gccgtttgtt
    11701 agaaaacggt gaaaaagttc ctgccgctgt aaaagcaggc gaccaagtta tgttcgaaaa
    11761 atacgccggt acagaagtaa aatacgaagg aaaagaatat ctgatcgtcg caggaaaaga
    11821 tatcatggcg attgttgagt aaatttggaa gataaaaaaa taatcaaaaa taatcaatga
    11881 ggtgaatcaa tcatggcaaa agaattgaaa tttgcagaag acgcacgcgc agctatgtta
    11941 cgcggggtag ataaattagc agatacagtg aaagtaacat taggacctaa aggccgaaat
    12001 gtcgtactag aaaaatctta cggctctcca ttgatcacaa atgatggtgt gacgatcgca
    12061 aaagaaatcg aattagaaga tcattttgaa aacatgggtg caaaacttgt atcagaagta
    12121 gcatcaaaaa caaatgatat tgcaggagac ggtacaacga cagcaactgt tctgacacaa
    12181 gcaatcgttc gcgaaggttt aaaaaacgta actgcaggtg ctaatcctct aggcattcgt
    12241 cgcgggatcg aattggcaac aaaagcagca gtcgaagaat tgcataatat ctcaactgtt
    12301 gttgattcaa aagaagccat tgcacaagta gcagctgttt cttcgggttc tgataaagtt
    12361 ggacatttga ttgctgatgc aatggaaaaa gttggaaacg acggtgtcat cacaatcgaa
    12421 gaatcaaaag ggatcgaaac agaactagat gttgtagaag gtatgcagtt tgatcgtggt
    12481 tacttatcac aatatatggt aacagataac gacaaaatgg aagctgttct agaaaaccca
    12541 tatatcttga tcacagacaa aaaaatctct aacatccaag atattctgcc attgttagaa
    12601 caaatcttgc aacaatcacg tccattgttg attattgctg acgatgtaga tggtgaagca
    12661 ttgccaactc ttgtattgaa caaaatccgt ggaacattca acgtagtagc tgtaaaagct
    12721 cctggtttcg gcgatcgtcg taaagcaatg cttgaagata tcgctatttt gacaggtggt
    12781 acagtaatta ctgacgatct aggtttagaa ttgaaagatg taacaatcga aaacttagga
    12841 aatgcttcta aagttgttgt agataaagac aatacaacga tcgtagaagg ttctggagaa
    12901 aaagaagcaa tcgaagctcg tgtgcaattg atcaaaaacc aaattgctga aacaacttct
    12961 gattttgacc gcgaaaaatt acaagaacgc ttagctaaat tagcaggtgg tgtagctgtt
    13021 gttaaagttg gtgcagctac tgaaacagaa ttgaaagaat taaaattacg cattgaagat
    13081 gcattgaatg ctacacgtgc cgcagtagaa gaaggaatgg tctcaggtgg tggtacagct
    13141 ctagtgaacg ttatcagcaa agtttctgct gtagaagcag aaggagacgt ggcaacaggt
    13201 atcaagatcg ttgttcgtgc tttagaagaa ccaattcgtc aaatcgctga aaacgctggt
    13261 tatgaaggat cagtgattgt tgataaattg aagaacgtgg aactaggcac tggtttcaat
    13321 gctgctactg gagaatgggt aaacatggtt gaagcaggta tcgtagaccc aactaaagta
    13381 acacgttctg ccttgcaaaa tgccgcttcc gtttctgcct tgttattaac gacagaagca
    13441 gtcgttgcag ataaaccaga accagcagcg ccagcagctc ctgcaatgga tccatcaatg
    13501 atgggcggta tgatgtaaga aaaagcaact tgcttatcta caactgatca ttagtgaaag
    13561 acagataaaa agaggctgga acagaagcgt ttaactccaa gaaataagaa gaaattcacg
    13621 gaaattgctt ttcaaatttt tgtgaatttc agcttatttc cgaaggagtt gcttctgctt
    13681 ccgctgttta tacgtgttta gagcgtgaga caaaagtgtt ctttactttt gtcccacgct
    13741 cttttttgtg tgtaattata atgaaaatag ttatttttaa tagttataat attttatcta
    13801 ttaaaagtaa atatccatt
//
LOCUS       DANKEF010000063        13816 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000063 DANKEF010000000
VERSION     DANKEF010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.063"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PWZ48_002239"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="PWZ48_002239"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDL2261882.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="PWZ48_002240"
     CDS             complement(2260..2511)
                     /locus_tag="PWZ48_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HDL2261883.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="PWZ48_002241"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="PWZ48_002241"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HDL2261884.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="PWZ48_002242"
     CDS             3461..3679
                     /locus_tag="PWZ48_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261885.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="PWZ48_002243"
     CDS             complement(3968..4480)
                     /locus_tag="PWZ48_002243"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDL2261886.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="PWZ48_002244"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="PWZ48_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDL2261887.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="PWZ48_002245"
     CDS             complement(6933..7376)
                     /locus_tag="PWZ48_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HDL2261888.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="PWZ48_002246"
     CDS             complement(7377..8165)
                     /locus_tag="PWZ48_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2261889.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="PWZ48_002247"
     CDS             complement(8162..9103)
                     /locus_tag="PWZ48_002247"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HDL2261890.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="PWZ48_002248"
     CDS             complement(9280..9867)
                     /locus_tag="PWZ48_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HDL2261891.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="PWZ48_002249"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="PWZ48_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HDL2261892.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11698..12708
                     /gene="gap"
                     /locus_tag="PWZ48_002250"
     CDS             11698..12708
                     /gene="gap"
                     /locus_tag="PWZ48_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDL2261893.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(12793..13728)
                     /locus_tag="PWZ48_002251"
     CDS             complement(12793..13728)
                     /locus_tag="PWZ48_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2261894.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         3810 a         3095 c         2436 g         4475 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgtaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgtattaat tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg
    11701 acagtaaaag taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc
    11761 aaagaagtct cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg
    11821 ttagcacaat tattgcaatt tgactctact catggtactt atcctggcac agttactgca
    11881 acagaagact caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca
    11941 agcaaaatcc cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt
    12001 tatacatctg aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt
    12061 tctgccccag ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat
    12121 gaaaacgaca aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct
    12181 tatttcttaa ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac
    12241 acttccacac aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt
    12301 tcagctgctg acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt
    12361 atccctgagc tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt
    12421 tcattaactg aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa
    12481 gcaatcaaaa aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt
    12541 tctagcgatg tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta
    12601 acaactgctg gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga
    12661 tttacttgcc aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca
    12721 atcgataaaa gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac
    12781 ttttaaactc gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt
    12841 ttcaggatct gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt
    12901 tggtcccttc gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc
    12961 gtctccatgt ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc
    13021 ttcaccgaaa aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg
    13081 aaatgctgga agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga
    13141 ataaccgcaa tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat
    13201 cataactgtt gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt
    13261 gttttctagc actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc
    13321 aatgtaatcc ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc
    13381 catatcatga aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg
    13441 agccacaaga gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt
    13501 cagaccgtct tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt
    13561 atgtgcatcc gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc
    13621 tttctcagaa ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac
    13681 aatcagagct aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc
    13741 tcatttctct tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa
    13801 tgttcatttt tataac
//
LOCUS       DANKEF010000064        13783 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000064 DANKEF010000000
VERSION     DANKEF010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.064"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            230..472
                     /locus_tag="PWZ48_002252"
     CDS             230..472
                     /locus_tag="PWZ48_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3781 domain-containing protein"
                     /protein_id="HDL2261895.1"
                     /translation="MENELLENLGRLHTTELGVIRIKKNLSLNVENVIEWCKEKISLS
                     NAEITRKGKNWYITIDNCIITVNAYSYTVITAHKVK"
     gene            469..939
                     /locus_tag="PWZ48_002253"
     CDS             469..939
                     /locus_tag="PWZ48_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002606082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HDL2261896.1"
                     /translation="MINFSIKELNAFAVIGQEVELTNYQKKNIQISTQFWRKFNSSLK
                     KAYLSQSGNWVKYAFMERRNGKLYYFCSIPQRTITPEGFLYKEIPSYKYLVVEHIGAM
                     DKIYETYGKIYEEIIPSTSYIPIKDIILHFEKYDYRFHWNSDNSVIEIWIPIQD"
     gene            1067..2281
                     /locus_tag="PWZ48_002254"
     CDS             1067..2281
                     /locus_tag="PWZ48_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDL2261897.1"
                     /translation="MNDKNFIEELRQKREEYGVTQTRLAVACGISREYYNRIEKGKQP
                     LNDELREIIEKQIERFNPQEPLFLLIDYFRVRFPTTDALAIIRDVLQLKSNYMLYEDY
                     GKYGYESKYVLGDINIMCSMQEHLGVLLELKGKGCRQMECYLLAQERSWYDFMLDCMT
                     AGGVMKRLDLAINDKAGILDIPKLKEKYKAGECMSYFRMQKDYSGTEKCGSDLPKNTG
                     ETLYLGSTSSELYMCAYQKNYEQYVKNGTEIEDTEIKNRFEIRMKNERAYYAVVDLLT
                     YRDAERTAFSIINHYVRFVDREDDKPKSQWITNDDWAWFVGENREPIRLTTKPEPYTL
                     QKALHWLQRQVAPTIKMVQALDRENRTTILKDMIEQAELKDKHKHLLQLEKSTIEERI
                     DTAVPQENDGIF"
     gene            3270..3479
                     /locus_tag="PWZ48_002255"
     CDS             3270..3479
                     /locus_tag="PWZ48_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008394616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA wybutosine-synthesizing protein 3-like
                     protein"
                     /protein_id="HDL2261898.1"
                     /translation="MNCLERFYRKGVCVIDNAVKTIQKKNGKCGIGNRGKTKIVVKEH
                     FSEKGKTMEELLTDVMLEKAKQTIA"
     gene            3591..5204
                     /locus_tag="PWZ48_002256"
     CDS             3591..5204
                     /locus_tag="PWZ48_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003019515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2261899.1"
                     /translation="MKQQIYNTALYLRLSRDDELQGESSSITTQRSMLRLYAKEHHLN
                     VIDEYIDDGWSGTNFDRPSFQRMIEDIEAGKINCVVTKDLSRLGRNYIMTGQYTELYF
                     PSHNVRYIAIDDGVDSEKGESEIAPFKNIINEWVARDTSRKVKSAFKTKFAEGAHYGA
                     YAPLGYKKHPDIKGKLLVDDETKWIIEKIFSLAYQGYGSAKITKQLRAEKVPTASWLN
                     FTRYGTFAHIFEGKPESKRYEWTIAHVKAILKSEVYIGNSVHNMQSTVSFKSKKKVRK
                     PESEWFRVENTHEPIIDKEVFYRVQEQIKSRRRQTKEKATPIFAGLVKCADCGWSMRF
                     GTNKTNKTPYSYYACSYYGQFGKGNCSMHYIRYDVLYQAVLERLQYWAKAVQQDEEKV
                     LNKIQKAGNAERIREKKKKASTLKKAENRQNEIDRLFAKMYEDRACEKITERNFVMLS
                     SKYQKEQIELEQQITSLREELSKMEQDMIGAEKWIELIKEYSVPKELTAPLLNAMIEK
                     ILIHEATTNEDNERIQEIEIYYRFIGRVE"
     gene            5239..6450
                     /locus_tag="PWZ48_002257"
                     /pseudo
     CDS             5239..6450
                     /locus_tag="PWZ48_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            6473..7399
                     /gene="glsA"
                     /locus_tag="PWZ48_002258"
     CDS             6473..7399
                     /gene="glsA"
                     /locus_tag="PWZ48_002258"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDL2261900.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            7607..9067
                     /locus_tag="PWZ48_002259"
     CDS             7607..9067
                     /locus_tag="PWZ48_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261901.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIREIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            9288..11051
                     /locus_tag="PWZ48_002260"
     CDS             9288..11051
                     /locus_tag="PWZ48_002260"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDL2261902.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            11135..11593
                     /locus_tag="PWZ48_002261"
     CDS             11135..11593
                     /locus_tag="PWZ48_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDL2261903.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            11702..12385
                     /locus_tag="PWZ48_002262"
     CDS             11702..12385
                     /locus_tag="PWZ48_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2261904.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            12532..13146
                     /locus_tag="PWZ48_002263"
     CDS             12532..13146
                     /locus_tag="PWZ48_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDL2261905.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            13162..13368
                     /locus_tag="PWZ48_002264"
     CDS             13162..13368
                     /locus_tag="PWZ48_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261906.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
BASE COUNT         4799 a         2173 c         2865 g         3946 t
ORIGIN      
        1 aattgattgt cgttttcttt tgcagaaatt taagtgcagg caattatggt agttgtcgta
       61 attgctgtca gctcagtgtt tatcactgat ggtgtgaaag atttacggtt ggagatgttc
      121 aataagatta ttggtgctta attattttta ttgtagtgct gatatgagaa tgttataatt
      181 ggggtataaa acatacaaat aaatttgctg aaaaatgagg tatcaaaata tggaaaatga
      241 attactggaa aacttaggaa gacttcacac aactgaactt ggcgttatta gaattaaaaa
      301 gaacttatct ttgaatgtag aaaatgtaat tgaatggtgc aaagaaaaaa ttagcctttc
      361 taatgcggaa atcacaagaa aaggaaaaaa ctggtatata acgattgata attgtattat
      421 aacggtaaat gcgtacagtt ataccgttat cactgctcac aaggtaaaat gataaatttt
      481 tctataaagg aattaaacgc atttgcagtt attgggcagg aagtagaact gactaattat
      541 caaaaaaaga atattcagat tagtacacag ttttggagaa agtttaacag tagtttgaaa
      601 aaagcgtatc tttcacaatc aggaaattgg gtaaaatatg cttttatgga aagaagaaat
      661 ggaaaacttt attatttctg ttctattccc caaagaacca ttaccccaga gggctttctg
      721 tataaagaaa taccgtctta taaatatttg gttgtggagc atattggtgc tatggataaa
      781 atatatgaaa cttatggcaa gatttatgaa gaaattattc ctagtacatc gtatattcct
      841 ataaaagata ttatcctaca ctttgaaaaa tacgattatc gttttcattg gaatagtgat
      901 aattcagtta ttgaaatttg gatacctatt caagattaga agcaatttta tatccataca
      961 cacaaagcag ttagtcttag gattgactgc tttttctata cagattttta atgacaggca
     1021 attatcaatt atggtagttg ccttttttga ttggaggaat tgaagaatga atgataaaaa
     1081 ctttattgaa gaactaagac aaaagcgtga ggaatacgga gtaacacaaa caaggcttgc
     1141 tgttgcctgt ggtatcagcc gtgaatatta caaccgcatt gaaaaaggaa aacagccatt
     1201 gaatgacgaa ttaagagaaa tcatagaaaa acagattgag cgtttcaatc cgcaagaacc
     1261 actattctta ttgattgatt actttcgtgt ccgctttcct actacggacg cattggcgat
     1321 tatccgtgat gtattacaac tgaaatctaa ttacatgctt tatgaagatt atggaaaata
     1381 cggatatgaa agcaaatatg tacttggcga catcaatatc atgtgttcca tgcaggagca
     1441 tttaggtgtt ttattggaac tgaaaggcaa aggctgtcgg caaatggaat gttatctgct
     1501 ggcacaggaa cgctcatggt atgacttcat gctggattgt atgacggctg gtggtgtgat
     1561 gaaacggctt gatttggcta tcaatgataa agcaggaata ttggatattc caaagttaaa
     1621 ggaaaaatac aaggctggcg aatgtatgtc ctacttccgt atgcagaaag attacagcgg
     1681 tacagaaaaa tgcggtagcg atttaccaaa gaatacagga gaaaccttat atctcggttc
     1741 aacaagtagc gaattatata tgtgtgctta tcagaaaaat tatgagcagt atgtcaagaa
     1801 tggcacagaa attgaagata cggagattaa gaaccgtttt gaaatacgca tgaagaacga
     1861 acgagcctat tatgcggttg tagatttact gacctatcgg gacgctgaac gcaccgcttt
     1921 ttctatcatc aatcattatg tccgctttgt tgacagagag gacgacaagc caaaaagtca
     1981 atggataaca aatgatgatt gggcatggtt tgtaggggaa aacagagagc cgatacgctt
     2041 aaccacaaag ccagagcctt acactttaca aaaagcgttg cattggctac aaagacaagt
     2101 cgcaccaacc ataaaaatgg tacaggcatt agacagagaa aatcgtacaa cgatactgaa
     2161 agatatgatt gagcaggcag aactaaaaga caagcataaa catttattac aattagagaa
     2221 atcaaccata gaagaacgta tagataccgc tgttccacaa gagaatgacg gtatttttta
     2281 atgtaattga aaaatttcag atttagtcag caaagagctg ttttcattcc ctatgtgaag
     2341 tgaaagaaga aaaataaatt ttttattaaa tctgcaacaa aatggctact tgcgtacaga
     2401 tatggtgcga gaaaaggaaa tctaaacttt ttttgaaaat aggtcattaa atcggggctt
     2461 gctgtcccta tatgaagtga aagaagaaaa aagttttgaa aaaatgacgg aaattcgtga
     2521 ttagtggaca gtcttctgtg aaaagaggaa aaacatcatc attttgtatt caaacaggca
     2581 ttagcagttg agtgttgatg tgaaaagatg aaaatacttt gttctcaact taacaattta
     2641 aggtttccgt tccctatatg gtgcaaagaa aatatttcat tctattgttg gcagtaacgg
     2701 tatggtttgg gtagttaggg tgtgaaaagg aaaatattct tttttcatca tcaacttagc
     2761 aaaatgacat tttctatccc tatatggtgc atgaaaagaa gttaaaattt ttccatttaa
     2821 acttagcaaa tggacgtttg ctgtacagat agtagtgaaa aaggagaaaa tcaaccgttg
     2881 attgccaaaa tgaaccgttc catttgtact aataatggaa aaagttttgg attttggtta
     2941 cgtttctcct cttttccaac gcgatatata ggaaagacga tttttttctt tcaatcgttc
     3001 tttgacaact gaataaagct attcaatacg tttgacagac agctagccgt tgaaacaaat
     3061 acgccacgac ctttcccctg caagagcgag cgaaaaccta tttgtgttct tgtaaaagat
     3121 ttgtcctctt ttatcgccat gacctgtact gtcagataat gatactcccg tacagccaca
     3181 gcttgagcat taagagcgtc gcacgcaatg ggtacggcta cataagaact atgcagaggt
     3241 ggaactcctg tgggctatga caaaagccgt tgaattgctt agaaagattt tacagaaagg
     3301 gggtatgtgt tattgataat gctgtaaaaa caattcaaaa gaaaaatggc aagtgtggca
     3361 ttggcaacag aggaaagaca aagattgtag taaaagagca tttttccgaa aagggcaaaa
     3421 caatggaaga acttctaact gatgtcatgc tggaaaaagc aaagcagaca atcgcataaa
     3481 atcggtgcag ttgtaagaaa tagattgaat gacaaaaagt acccgtgtta tactttactt
     3541 gcaagcaggt attgtaaaca cgggttagtt ataaaggagg ataactgaca atgaaacaac
     3601 agatttacaa tactgcactt tatcttaggt taagccgaga tgatgaatta cagggcgaaa
     3661 gttccagcat taccacacag agaagtatgt tgcgtttata tgcaaaagaa catcatttga
     3721 atgtcattga tgaatatatt gatgacggct ggtcgggaac aaattttgac agaccgagtt
     3781 ttcaaagaat gattgaggat atagaggcag gaaaaatcaa ctgtgttgta acgaaagacc
     3841 tttcccgtct tggcagaaat tatattatga caggacaata tacagaattg tatttcccta
     3901 gtcataatgt ccgctacata gcgattgatg acggtgtaga cagcgaaaaa ggcgaaagtg
     3961 agattgcacc atttaagaac atcatcaatg aatgggtggc aagagataca agccgtaaag
     4021 tcaaatcagc atttaagaca aagtttgcgg agggggctca ttacggtgct tatgctccgt
     4081 taggatataa gaaacaccct gacatcaaag gaaaactgtt ggttgatgat gaaacaaaat
     4141 ggattattga aaaaatcttt tccctagcct atcaaggtta cggaagtgca aaaatcacaa
     4201 agcagttaag agcagaaaaa gttccgacag cgtcatggct aaattttaca agatacggta
     4261 cttttgcaca tatctttgag ggaaaacccg aaagtaagcg ttatgagtgg acgattgctc
     4321 atgtcaaggc gatattgaaa agtgaggttt atatcggaaa cagtgttcac aatatgcagt
     4381 ctacggtatc gttcaagagc aaaaagaaag tgcgtaagcc cgaaagtgaa tggtttcgag
     4441 tagaaaacac tcacgagccg attattgaca aggaagtgtt ctatcgtgtg caggagcaga
     4501 taaaatcaag acgcagacag acaaaggaaa aggcaacgcc gatttttgca gggcttgtca
     4561 agtgtgcgga ttgtggttgg tctatgagat ttggaacgaa taagacaaat aaaacgcctt
     4621 acagttatta tgcttgcagt tactacgggc agtttggcaa aggtaattgt tctatgcact
     4681 acattcgtta tgatgtgctg tatcaagccg tattggaacg cttgcagtat tgggctaagg
     4741 cagtacagca ggacgaagaa aaggtattga acaagataca gaaagctggc aatgcagaga
     4801 gaatacggga aaagaagaaa aaggcaagta cattgaagaa agccgagaac agacaaaatg
     4861 agattgaccg tttatttgcg aaaatgtatg aagatagagc ctgtgagaag ataacagagc
     4921 gaaattttgt gatgttgtcc agcaagtacc aaaaagaaca gatagaactg gaacagcaga
     4981 taacaagcct aagagaagaa ctaagtaaaa tggaacagga tatgataggt gctgaaaagt
     5041 ggattgagtt aatcaaggaa tattccgtac caaaggaact gacagcacca ttattaaatg
     5101 ccatgataga aaaaatcctc atacatgaag caacaacgaa tgaggataac gaaagaatac
     5161 aggaaattga gatatattac cgatttattg gaagagttga gtaatcaaca agagtaatat
     5221 ttttaactaa gggaaaccgg cggtgtctat acttgggtca gtcgcacatt gggaaaacga
     5281 ttcggttttg ccgcaatctt tttccaatgg tttgagataa cagttggcta cttgaccatg
     5341 ctgtatttct taactggtgc attatcctat gcaacgggta ttggtgctat acaaaacaat
     5401 aaatatctga aattggcaat cctattgatt attttctggg ctattttact ctcacagtta
     5461 aaaggaacca aatacacttc attgattgct cggatcggtt ttgtagctgg tatcttgttg
     5521 ccagcattag tcttgtttgg cttagggatc cattatgtcg cttcaggcgc tccactacaa
     5581 acgatactgt ctatgaaaac attgattcct gatttttcga aattgcctac gctggttgtt
     5641 tttgtttcct ttattttagc atatatgggc gtagaaacat ctgccagtca tgcaaatgaa
     5701 atggaaaacc ctaaaaagga ttatccatta gcaatgtttg tattggttat tctggcaatc
     5761 attttagata cttttggcgg attgaccgtc gctactacag tacctcaaca tggtttgtct
     5821 cttaatacag gggtgattca atcgttagaa ttcctattaa gacacattga tccaaatctt
     5881 gcatggattg cgaaaattct tgcgattctt gtttgtttag gtgtcatcgg agaaattgct
     5941 tcttgggtaa cctctccttc aaaagcactt cacgtagcag cagaagaagg attacttcct
     6001 gaatattttg caaaagaaaa cacacacggt gttcctgctc atttgatgat cgcaaatggg
     6061 atcgttgcaa caatctgggc agccgtcttt acattaagcg gcggggggaa taatctttca
     6121 tttcttgcag cgatgtcatt gactgttgta atctacctga tgttgtactt tatgtttttc
     6181 gcgggctatt tgaagctggt ttttaaagat gacagtcttg aaagagccta tcaggttcca
     6241 ggcggaaaag ttgtaaaagt aatcgttgct tgtttaggtc ttttgacttc actttttgct
     6301 ttttgtatct catttgttcc acctgctagt atcgcagctg gacagcatgg gatttatgaa
     6361 acgattctga ttgtttctta tcttatcgtc ttagttattc catttacgat ctacgcatgc
     6421 cgtcataaat tccagcatgc agaaaaataa aaaatgaaag ctggttattc aaatggaagt
     6481 aaaagaatta gaacaattga ttggaaaaaa tttccactat tatcaaacag gacaagtggc
     6541 ggattacatt ccagcattag ctgaagtgga tcccaaacaa ttaggtatgg cgatttatga
     6601 tttggataca aaacaactga ttgagggagg agacgccgat gtgcgctttg ccatcgaaag
     6661 catgtcaaaa gttcccgtat tgttattagc aatcaaagac aacggaatag aaaaagtctt
     6721 tcaaacaatc aacacggaac caaccggttt tgccttcaat tctatcatga atatggaaat
     6781 caatcatcgc aaacatccaa tgaatccttt tgttaatgca ggagcgattg caacaacctc
     6841 gttgattgcc ggtaaagatg cagatgaaaa atttgagcga atcttgtcat ttatgaaaga
     6901 gatctgtgat gatcctaata tcacattgaa tgaagaaatc tatcattctg aatcaagaac
     6961 aggtgatatc aatcgctctc ttgcttacta tatgaaaggt aatcaaatga tcgaaggaga
     7021 cgtaccggag atactcgatg cttatttcag acaatgctcc ctcaatgtca ccgctacagg
     7081 catagcaaaa ttagctgctg tgctggcaaa taaaggaatt gctccttgga atggcaaaca
     7141 aatcattact gaagaaagtg ctacaatcgt caaatcaatt atgacaactg ctggtttgta
     7201 tgatgaatca ggagagtttt ccgtccatgt tggtgtacct gcaaaaagcg gtgtaggcgg
     7261 cgggttgatg gcagctgtgc caaatcgtta cggcataggt gtcttcagcc cggcactaga
     7321 tccatttggt aacagcgcag ctggtatcca attgttgaaa gatgtagtca aagaactaga
     7381 cgctgacatc tttgaataac cattcttgaa agaggctggg gtaaaagtcc aaacggctca
     7441 aagctaccta caatgaggct gaaattgtga gcttgtttga cttttgtccc agtttctttt
     7501 tttattggtt tttgaaaggg cttttctaaa taaaaaggaa acaaaaatac gatttacaga
     7561 tatgattaaa taaaatcata agaaaaactg gaggcgtgta tcaggaatgc tggaaaatta
     7621 catcgagcga aatatattta gaaaagtgta tctgtgtgaa caattatttg aattccaaga
     7681 aatagacatc gaacaaacag ctatttctct aagggtgact acaccaacga tccttcacga
     7741 tctagaaagt ctagccgagt gtctagaata ttgtataaaa gaacaagtaa gagagaagca
     7801 taagtataaa ctcgtattca aacatggtat tgctctttct gagctcacac agtttctgta
     7861 cggtcaatct tattttttaa agtttttaag ttattatttt aatggtatat tcacgagtac
     7921 agaactggct gatcttgaat ttatttcttt gagtaaagta tatacaatca aaaaaatcgt
     7981 attggatttt tttaaagaaa atgattattt gaaaaataaa gaaatcatta tcccagaatt
     8041 tgactcgcgg aacatcttgc tagctttagt acgttatgtc aattgggaag gctatgaaaa
     8101 taaaaatccg tatgtagaag aagcttgtaa cgagttgatt gattatgtag agactcattt
     8161 ttttaaaaga agatattcgg aagatgaaaa gtcctttatc atacgagaaa tagagatcgc
     8221 aatcggaaga aaggcatttc cccttggact gaaagaagaa gataagaaaa gcgcagaaac
     8281 gaagccgtta tttcaattag tctgtcaagg attggaagag caaaaaggaa ttctagactt
     8341 acaagaagaa gatgtctatt atattttttc tttgttcaac agtagaaatt ataccaatga
     8401 gaacatggag ctgttaagaa aagatttcga agttgtttat aaaaattttg ttctcaatga
     8461 cctatcactg aatgaattga ttttacagat tattagtatc gctgaaatcg acgatgcaga
     8521 aaattttatt ttcaagcaat cctttttaca atttgtccgt acgttgtggg cagatggaca
     8581 agtattttta ccggaaagga tctatcttct atcagaaaaa gaacaacttc tgtacggaaa
     8641 aattgtagaa atattggaag agtggaaaaa gaaaaatcaa atccaactaa gatggaatga
     8701 aaatttcatt cgaaaattcg tcaaaagtct ttctttgatc aacagtgaac aagcggggag
     8761 acaggaaata gaaatttttg ttgtttcaga aagttcagtc aaacaatttt tttatcgaac
     8821 ccaatttcat ttgtatgcgg aagaaggcat cgctgaaata aatcctattt tatttcgttc
     8881 attagaagaa ttgcctgatg aatgtctatg tgcgaaaaaa agagtggttt tgtgcgatgc
     8941 tgctagctat caaaatggat tagaaactga aaagacaaag atctatccaa tctcattaaa
     9001 aggtgcaaac atccatttgt atcatattgc tcaggaactt cacctattaa aaaaacatat
     9061 ttcttgatga gaccttaatt aggagacctt ctacaaagct ttttaattgt ctgtagaagg
     9121 tttttatttg agtcctgatt cgtgcgaaga tctacttata taacaagctt tctctgaaat
     9181 ttttttaaaa tgctattgac ttagagtcaa ctctaagtag tagaatagat aaagtgtaac
     9241 aaatctattt tcattaaaag aagatagtac ggaggagaga atgtattatg agtacagtat
     9301 tacatgtatt ggatgcaatc aatagccccg cagatgtcaa aaaattatct gttgaggaaa
     9361 tgaaacagtt ggcagaagag atgcgccaaa aaatcttgca aaaggacagc gatgtaggtg
     9421 ggcacgttgg tccgaatcta ggtgtagtag aattaacgat tgcgtttcat tatgtatttg
     9481 attcaccaaa ggataaaatc gtttgggatg tttcccatca gtcttatggt cataagcttt
     9541 taactggtcg aaaaaatggt tttgaaaaag gacattaccg tgaagtgagc ggatattcca
     9601 accaacatga aagtaaacat gactatttta cagtggggca cacatctaca tctatcagct
     9661 tagcaacagg tttagctaaa gcgagagatc tgaagaaaga atcaggaaat gtggtagcat
     9721 ttatcggtga cggctcccta agtggtggct tagcttttga aggattgaac aatggtggtg
     9781 ttttacaatc caatctaatc gtattagtaa acgataatga gatgtctatt gatgagaatc
     9841 atggcagttt atataaaaac ttagctgagc ttcgtcaaac caacggtaaa agcagcaata
     9901 acctatttaa agcaatggga tttgattatc gttacgtgga taatggaaac gatctggaaa
     9961 cggtgatccg tttatttgaa gaagtaaaag atattgatca tccgattgta cttcacgtcc
    10021 atacggaaaa aggtcgcggt tatcagccgg caatcgataa taaaatgaaa tatcattggc
    10081 atgtaccatt tgatctgtcc accggggagg caaagcaagt aacttctgga gaaacattta
    10141 atatgatcat catggattat ttggataaga aaatgcaaac aagtacagct cctattgccg
    10201 cagtcaatgc agcgatacca ggcgcctttg gactgaaaac attccaagaa aaacatccag
    10261 atcattattt tgacgtgggg atcgctgaac agcactctat cactttcgct tctgctttgg
    10321 cttctcaggg agtacgtcca gtcgtcttcc aatcaagtac tttcttgcaa cgggcatatg
    10381 atcaattatc acatgattta gcgatcaacg agaatccagt gattatttta gtcggtggcg
    10441 gaacgatctc agctagtgac cgaacacacc aaggaacatt tgacattcct tacttatcaa
    10501 gtatcccgaa catcaaatat cttgctccag caacaaaaga ggatatgatc tctatgctag
    10561 attgggcgtt cgaacaaaca gcgcagcctg tagcaatccg aattcctaca catggtgtag
    10621 aacatggtac agctatcgca aatgattata gtattccagc ctatcaaaca gtaaataatg
    10681 gaggaaaagt agcaatttta gctttaggcg gttttcttga attaggaaaa gaaacagtaa
    10741 aagcttttag aaaaaaagga atcgaagcaa cgttgatcaa tccaggaacg atcacagaaa
    10801 atgacgtaga aacattagat aaattaaaag aaacacacga attagtcatc acattagaag
    10861 atggtagttt gtcaggagga tttggcgaaa atatcagtcg ttattatggt gattcttcaa
    10921 tgaacgtatt aaattttggt gcagagaaag aattcacgga tagcgttcct gtagggcagc
    10981 tttatgaacg ttatcattta acaccaaaac aaatcgttga agatagtctg cgtgtcttga
    11041 atattcaata attcattatg ctcaccatcc agataattgt ttatactagt atctggatgg
    11101 ttttttcatt ataaaaagca aaggggtgga tgttatgtat cagcaagtag aagaatatgt
    11161 caaaaagttc caagaaaaat atcctagtta tcaatcagaa aaattaggtt tcttttcatt
    11221 tggggataca gaaaaaatgg cagacgagtt gagcgaacta gttgtaaaaa agataaagac
    11281 tggtacatca agtggttacg atctgtataa acaagagaat gagcctttac caaaagttgg
    11341 tcaattagac gtggtcctga atggaaaaaa tgaaccagtt tgtatcatca aaaatataaa
    11401 tgtgaccatc ttgcctttta gtgaagtcac gaaagagcat gcttggaaag aaggagaagg
    11461 ggatcggaca ttagcttatt ggcggaaagt ccatgctgag ttttttaaac atgcttataa
    11521 agaagcgttg aacatagaat ttacagaaga gaagctggtc gtatacgaag aatttgaagt
    11581 aattttcttt tagtaagaaa ggtctgctga aagaaatagc tcattattgt ctcctgaata
    11641 gaaaagggtt acatttttat atgaagctgt atttttatat aacattactg gaggagatat
    11701 tgtggataca ctggaacaaa tcaaaggcgg agtcgtcgtt tcttgtcaag ctttggaaaa
    11761 cgagccgcta cacagttctt ttattatggg gagaatggct ttggctgcaa aagaaggcgg
    11821 agcagttggt attcgagcaa atacgacaga agatatcaat gaaatcaagc gaatcgttga
    11881 tttaccggta atagggatta tcaaaagaga ttacgatggg tcagaagtgt ttattactgc
    11941 gacagaaaaa gaagtggaag aacttttgga gacgcaagca gaaatcattg cactggatgc
    12001 aacttctcgt aaacgtccga cagaaaaaac aactgctgaa cttgtccaaa tgattcacga
    12061 acacggtcgg ttagctatgg cagatatttc gacttttgag gaagcgcttc aagcgcaaga
    12121 agatggtttt gatcttttgt ctacgacttt agcaggctat acagaatatt cacgaaagac
    12181 gcagacacct gattttgatt tattgaatca aatcatagaa caagtcgata tcccagtaat
    12241 tatggaagga catactgact ctcctgaaca agtagagaaa gcatatgaat taggtgcttt
    12301 ttcagtcgta gttggatcta ttatcacccg tccgcaattg atcactaaaa gatatgtcga
    12361 agccgcaagt aaagcaaata actaaaaaac acgcacggca cagattcttg acaaaaaaga
    12421 aaaattgata gatagtttaa agcagattct aagaagatga aaaaagcatt acttttcatt
    12481 ttgtcttcaa gatctgcttt ttaacgaatt ttatagaaag aaggcgaaac catgtttgaa
    12541 cggagtcctt cagaattgac agaacgtgaa aattacaagt tgctgattgg ttcagtcatc
    12601 cctagaccag tagcagtagt aacgactaaa tcagcaaaag atatagtgaa tatcgcaccc
    12661 ttcagttact tcaatatcgt cagttcgaat cgacccatct tatccttggc gatccagaga
    12721 aaacaagatg cagtaaaaga cacagcaaag aatattcttg agacaagaga agctgttatc
    12781 catatccttg atgaggacaa cgtagaagaa gtaaataaaa cagcagccaa tctgcctaat
    12841 gaggaaagcg aactgtacct gactgacttg actttaactg attcagtaat catttcagta
    12901 ccagcattga cagaagcaaa agttcgtttt gaaacatcct tatatgaaca tgtcgagata
    12961 cggaataaaa ataaagtaac agctgattta tttttgctag aagtacagaa atatcattta
    13021 aatgaaacag tctatgatga taagacagga aggatcgatc caagaaaatt gaatgctgta
    13081 agccgtttgg caggaaatga ttatgcagca ataggagaaa ttttcacgat tgcacgtccc
    13141 gaataaaaag gagcactagt tatgttaaaa ataagctata aaccatcgac agattcaaaa
    13201 gaaatgaaaa aagaatatga aacagtgaat gattttctgc aagggcagta cttagaagtt
    13261 ccgccattgc aagaccattt tgtcgttact actgttactt tagatggtaa ggaaatagaa
    13321 atgccagatc aaacgattag cggattgttt aattacttta acaaataaat atttattccg
    13381 aaaagtgaaa aaacatataa aaaaacacat aaaactaaaa aattatcaaa gaatcctggt
    13441 gcaactgaaa gaatttgcaa cgggattctt tcaacttatt tgttattttt ttctaaaaaa
    13501 tattaaagat tgttaaattt cactatttca tttcaaattc cattgcctaa tcattaggaa
    13561 aagtctataa tgaactcgta aatagaatat agtaataacc ttgttaggtg aggctcctat
    13621 acaaacatag gctgttgccc agaaacgtcg aaagtcgcca atgggtaaaa cagaaactgt
    13681 tggattaagg ctttttccaa tagctaagaa tgttttcttt acgttgtata gtgctaaaac
    13741 tcggacgatg agatgatgct aggtcagaat tcttgttcga aaa
//
LOCUS       DANKEF010000065        13733 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000065 DANKEF010000000
VERSION     DANKEF010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.065"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(63..1268)
                     /locus_tag="PWZ48_002265"
     CDS             complement(63..1268)
                     /locus_tag="PWZ48_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2261907.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
     gene            complement(1291..1444)
                     /locus_tag="PWZ48_002266"
                     /pseudo
     CDS             complement(1291..1444)
                     /locus_tag="PWZ48_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            complement(1447..2832)
                     /locus_tag="PWZ48_002267"
     CDS             complement(1447..2832)
                     /locus_tag="PWZ48_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDL2261908.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            complement(2861..3247)
                     /locus_tag="PWZ48_002268"
     CDS             complement(2861..3247)
                     /locus_tag="PWZ48_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2261909.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            complement(3263..3577)
                     /locus_tag="PWZ48_002269"
     CDS             complement(3263..3577)
                     /locus_tag="PWZ48_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HDL2261910.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="PWZ48_002270"
     CDS             complement(3930..4409)
                     /gene="rlmH"
                     /locus_tag="PWZ48_002270"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDL2261911.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            4837..6126
                     /locus_tag="PWZ48_002271"
     CDS             4837..6126
                     /locus_tag="PWZ48_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HDL2261912.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(6165..6770)
                     /locus_tag="PWZ48_002272"
     CDS             complement(6165..6770)
                     /locus_tag="PWZ48_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HDL2261913.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(6966..7430)
                     /locus_tag="PWZ48_002273"
     CDS             complement(6966..7430)
                     /locus_tag="PWZ48_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2261914.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(7452..7772)
                     /locus_tag="PWZ48_002274"
     CDS             complement(7452..7772)
                     /locus_tag="PWZ48_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HDL2261915.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="PWZ48_002275"
     CDS             complement(7915..8994)
                     /gene="pepA"
                     /locus_tag="PWZ48_002275"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HDL2261916.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            9194..9544
                     /locus_tag="PWZ48_002276"
     CDS             9194..9544
                     /locus_tag="PWZ48_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HDL2261917.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            9729..10682
                     /locus_tag="PWZ48_002277"
     CDS             9729..10682
                     /locus_tag="PWZ48_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDL2261918.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            10695..12482
                     /locus_tag="PWZ48_002278"
     CDS             10695..12482
                     /locus_tag="PWZ48_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261919.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            12469..13671
                     /locus_tag="PWZ48_002279"
     CDS             12469..13671
                     /locus_tag="PWZ48_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261920.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         3938 a         2689 c         2520 g         4586 t
ORIGIN      
        1 aggttttgtc caaaattcat aaaaatgttc tcctctctat attcaatgaa tttgtatttg
       61 agttattttt ttgttgttat cacgtcctgt tcttttactg actgttgctt caaaatctgc
      121 ttgtgtcggt ctgtcagttt cgcatggtcg agaatgtctt ttacaacctg cgtctggttg
      181 atttcatcaa gtttaatcgc aacctttaag gtcggggcaa cttgatgaga tagccagttc
      241 agcgtccttt ggaaggagta aggctctggt tttgtggtta gttttaatcg ttcacgattg
      301 ttcccaataa accaagccca ttcttcattc agtttccaat cagaacgagg tttggaatcg
      361 tctttatcta caaaacggat ataccgattg ataattttaa aggcggtatg ctctggattg
      421 tcatagacga gtaaatcacg gactgcataa taggcacgct catttttcaa tcgaatctca
      481 aaacggtttt ttacttctgc gtcttcaatg ggaatatcat ttttcttgta ctgctcgtag
      541 tccttttcat agatacagaa ataaacttca ctttgtaatg aaccgatata gagggtgttt
      601 cccatacatt ccttttcctc tttgcgtacc agttcgccac tgcgatagct tttaaaactg
      661 cggaagacgg agatacattc ttcctgttgg cacttttcag tgagtacagg gatatttaaa
      721 atccctgtct tatcgttaat ggcaaggtca aggcgtttca tcacaccgcc agccaccaaa
      781 acgtccataa agaactcata ccagcttctt tgttgtgcca gaagatagct ttcaaattgt
      841 ctgcacccac gacctttcaa ttccaccaga actcctttgt ccagttcatg ggagcaaagg
      901 acgaatatgt cgcctaaagc ataatgctct gaataagaat agaaaccata gtcctcatga
      961 agaaaatagg acagtttcag ttgtaagatg ttttcgacca cctgctgtac gtctgttgtc
     1021 ggaaagcgaa ttcttacata atcaaacagc atttcaaggg gagcgtcggg attgaagcgt
     1081 tccagagctt cccaaaggga ctgctgtaaa tcctctgatg gcttgacttt tcctgtttca
     1141 atatcgctta gatactgcct tgtaatacca gtcgcaacag ctaaacggtt ttgagatagt
     1201 ccataagcca agcgtttttc ttttaaatgc tgtaaccaag tttgttcatt cagtaaaaat
     1261 ccctccaatc aaaaaggcgt atgtcaactt ttaaagccca tttgacatac gctgaaattt
     1321 tgtaaatccc ttgtaaccaa aggattttct aatgtttttt tgactgtttc ctgtcgattt
     1381 gtacccccct gttagatacc gaggggttaa gtgctggcgt ggctattgcc acaccagcca
     1441 gcaagatcag tccacacctg cgacttccgc ttcgcacgtc gcctgcgtgg actgtctgct
     1501 gttggataac tttttaattt cctccaagaa atcatatcct tttggtacaa ggggagtata
     1561 aaactctgat atgacacttg ttcctacatc aacatagcca cgacctttga ttcgctttaa
     1621 gaagaaatcc ttttgtacgt cactgccaaa catcatgcca tagcccattt cagacatacg
     1681 acctaaagcc actctgaaat taaactgatc acggattccg tcgcctaaat attttgcgtc
     1741 tggacgttga caagccagta ttagaaagaa gccagcttga cgacctaaca tgacaatctg
     1801 tttcagctta ttcataactg cggtgttttc ttttgttccc agcatttcca tgaaagcgac
     1861 gtattcatca aagattaaga agtgtgccgg gagacctaag taagcataat ttttgccagt
     1921 cttatagttc ttcatctgct tcatttcctc actacgtttc atcatttctt catagaatgt
     1981 ttcaatgcaa gaaagcaagt cttcttttct atagtagaca tttgccatca cagaacctaa
     2041 gtccgcaagg tcagcatttt tcgggtcaag aatatacagt tttgaatctg tatgaagcaa
     2101 ggcttcaatc agtgtcagta taaagtaagt tttaccgcca cctgtaccac cagcaatcaa
     2161 catatgaggg agcttatcat attcccacca tacgtttttc attaagcgaa gtttaccatc
     2221 tttagcttct acttcatcaa tagaaatacg actggctatg gtgtcataga gcaaagtata
     2281 ttccacatag gaatccttta actctttatc cgtcagctca cagtacaagc cactctctaa
     2341 tttcttttcc aagtgtaaga gttggtcttg atattttccc agcgtgattt ccacccgtat
     2401 ctgtatcaag ccatttttaa gtcgataata cattttaggg aagtaggtta tcttttcctt
     2461 tgtacgacca gcactatctt taaagaaacc ctctgttttg acctgttcag attcatacca
     2521 cttgttttca agtatcatct ttgccagttt ttgacggtgg taaagttgtt taaccgtatc
     2581 atagcgaacc cgtttgaata caaacgctac cagcaagcag ataagaattg cgacactgaa
     2641 actgataatt aaatagggaa tgtcaatctt atctgcttgt gataggttaa aatcctgcca
     2701 gttgatctgc tggattgtct tcacatgaaa cagtccgaca accagcagga aaacaggcag
     2761 gagtgacgct atcgtaaaat gaaagactaa atctttacca gatgggcgaa tccttttacc
     2821 acgctgtttc atgcgaaaaa gtctcctttc tacctagcga ctatttgtct tgtgtcggtt
     2881 ctttctttgc ttgtggttga gctttgaatg aactagaatc ctttgtcagc acaatatcgt
     2941 ctgccttgat ataccagtca acatctgctc cttgataggt ggcagtagca acggtgtccg
     3001 caatgggatt gataagttcc acccgtgcgt tataatcaaa ctctttcaaa ggcacgctgg
     3061 caggaatact tacttgaatc atgcgtcctt gtcctttgga ttttaagtca taggtacgtt
     3121 ccttgatttc atctgaaacc gacccgtctt cattttggat tctcacttca cgacgtagag
     3181 cagagaattt caattctcca aaagtcgtgt ctttatctaa tacaatgcca tttgctaatc
     3241 tcatcatttt tcctctcttt ctttattctt ttatcatgtc gtcagcatgt aaaaggtaat
     3301 ttgtaaaacc acgagtgccg attttgtagc cctctgcggt aatacgtgga ttgactaact
     3361 tcacacgttc ctcaaagccg aaatgttttt cgccagcttc agcaggaagc accaccacaa
     3421 tatcatctgc tctttgaaca tcagaataga gattatagct tcttgataag acagttagcc
     3481 gtccgttgat tcttcgctga acgactttat cctcgccagc aaattctaaa ttgccgaatg
     3541 ttttttccat gttgggaatc acaaatttaa gttccatatt tttacctatc ctttcttttt
     3601 tattggctga atgaatgttt gatggtctta aagagtgggg aacgaccttt tgattcttga
     3661 ttttttgttt tcataagttc acttcctttc aaaatcgggt aaaaaaatag acacctcatt
     3721 ttttgaagtg tctacctatt aaatattcaa attttattgg aagtatcttt atatcttcac
     3781 ttttcaagga taaatcgtcg tatcaaagct cattcataag tagtaaatta gtagtaaatt
     3841 gagtggtttt gaccttgata aagtgtgata agtccagttt ttatgcggat aactagattt
     3901 ttatgctatt tttctcttaa aaaatatcat tatttgtggt atggagcccc cgcgttgatc
     3961 cggaaggcac ggtagatttg ctcgacgagg atcaggcgca tcaattgatg tggatacgtc
     4021 attttgccaa aagagatctg agcattgctt cttttcatga cggcttcgct gagtcctaaa
     4081 gaaccgccga taacaaaaac aaggtggctt tttccttgga tacctaattg gtcgatttgt
     4141 ttagcaaaag attcgcttgt gggatttttc ccttcaattg ctaagccata gacgtattct
     4201 tgttctttga ttttcgctaa gatccgttct ccttcttttt cttttacttg tctcatttgc
     4261 gcttcgctta aattctccgg ggctttttcg tcaggaactt cgatcaattc gattttcgca
     4321 taggcattca gccgtttgac atactcattg ataccttgga tcaagtattt ttcttttaat
     4381 tttcctactg aaataatttt gatattcatg ttttctctcc tttgatcttc acgctcactt
     4441 tgtttattat aatcaatttt ttcgtctata tgtttatttt gactattttt gttaaaaaga
     4501 tttccccaac agttatgcac aacatccaca ggtttatcca cagaaaacag cgttgtttta
     4561 ccttatttca tcacattcat taggcaaaca tacgttttat aagaatttat ccactttata
     4621 cacaggctgt ggatatgttt tatcccttat ttttccaatc ctatccgcgt agttttacac
     4681 aatatctgtg gaaaaagaaa atcaaatatt ttcatctgaa aacaatgatt tttcctaaag
     4741 tttttcataa aaagttcaaa gtctcaaagt agactaaagt caacaaataa gactatacta
     4801 acagtagata aatgaatgta attggaggag gaacacatgg atagaaaaaa tgtgacacca
     4861 aagatgaaaa aaaataaaaa taatagcctc tggcgtaagt tgggtctggg gcttgttgga
     4921 gggatcgttg gaggactact gacagctggc attttttatg cagcgatggg cactggcaac
     4981 aatagttcta ctgctacaag cggcaatcaa aattctgccg gtgaaacagt tgtggaaaat
     5041 gtcaaagtca atgtagattc tgatatcacc aaagcggtag ataaagttca agacgctgtc
     5101 gtttctgtta tcaacttaca aagtcaaaac caatcaagcg gttttggcgg attattcggg
     5161 cagcaagaag aaagtagcag ctcagatgat agtaatttgg aagcttatag tgaaggaagc
     5221 ggcgtaatct acaaaaaaga cgggaatact gcttatgtag tcacaaacaa tcacgtagta
     5281 gatggacagc aaggtttgga agtcatgatg aaagacggta cgaaggtaaa agctgaatta
     5341 gttggaacag atgcctattc agatttagcg gtgttgaaga tcaattcaga caaagtcgaa
     5401 acggttgcct cttttgggga ctcaagtgct ttgaaggttg gtgaacctgc gattgcgatt
     5461 ggttccccat taggatctga atatgcgaat tctgtcactt caggaatcat ttcttccttg
     5521 aaccgacaag taacaagtac gaatgaatcg aacgaaacag tcaatatcaa tgcgatccaa
     5581 acagatgcag cgatcaaccc gggaaattct ggtggtcctt tagtgaatat cgaaggacaa
     5641 gttatcggta tcaactcaag taagatcgca agtacttctg aatcgacttc taatgtaagc
     5701 gtagaaggaa tgggatttgc catcccaagt aacgatgtag tgaatatcat caaccaactg
     5761 gaaaaagacg gaaaagtgac acgtccagct ctaggtatca caatggtcga cttatctgcc
     5821 gtttctactc agcaacaaga acaaatcttg aagattcctt catctgtaac taacggtgtc
     5881 atcgtgacaa atgtccaaac agctacaccg gcagaaaaag ctgggttgaa acaatacgac
     5941 gtcattacta agatcgatga caaagaagtt tcttctggtg tagaacttca atctgctttg
     6001 tataagaaaa aagtgggaga ttctgtcaaa atcacttact atgaaggctc gaaagaaaaa
     6061 acaactactg ttgaattgac gatcgatcaa tcggcattaa aacaaaatca aaatagtgaa
     6121 aattaataaa aaagaggttg tgacaaagac ttgtcacaac ctctttattt tggaaatact
     6181 tctccgacta cggcatcctc aggcaatact aagattcctt ttttttctgg agcatttggc
     6241 aaatgcaatt cacgagcaga acaaatcatt ccatagctgg ctacccctcg caaagttcct
     6301 ggccagatca tcattccatc tggcatcatg gctcctggtt ttgcaacgac aactttttgt
     6361 ccagcttcga tatttggtgc accgcaaaca atttgcaata ctgtcccatt gtccacttct
     6421 gtctcagtaa tcgataaatg gtcagagtca ggatgcggga cgcatgtttt tacatacccg
     6481 acaacgatct tcggttcatg atcagcttca agctgttctt cgaatcctgc ttctttaatc
     6541 aatcgattca atttcgctaa ttcttcatct gttaagctta cttggccgtt cccttcaatt
     6601 gttagatgat cggagatatg gaaaaagttc caagcaacaa cttgaccttg ttcatctttg
     6661 atccgagcaa cattccattt acgttcgaca gtgttttctg ttcctttatc gtcggctatg
     6721 ataaccatca aagtgtctcc gacgtgtgct ttgttataag caaaaatcat gttctttcct
     6781 ccattatcct ttgctgagat ttatcttaac aaaaaattgc attcactgca tgagattttt
     6841 ttcatgcttt atttccgaag aagcttcttc tggaacacca tttattcgta acaagggact
     6901 gtgacaagac ttttgtcaca gtccctttac tgtttgatta aaaaggttat acaagttcca
     6961 cttgcttaat tactcggctg ttcttcttcg tcagaaacga tcaagacatc acaaggtgaa
     7021 tgacgtatga cgtagctggc tacacttcct gtcataaagc gttcgactgc atttagtcca
     7081 gactgcccaa ccatgattag atcgattgca tatttttttg gcaatttgac tgccatggat
     7141 tcacgaacag agccgtatgc aataattcct tctatatttg tgtaatcaac agattttcca
     7201 tattctttac attcttcgat catttgcttg gctgtttcag tttgctgatc gactagattt
     7261 tgatcaatag gagcatagcc cataaaatta taaaattgct gatcgattac gtttgctaca
     7321 tatactttcc cattattacg gcgagccact tctaacgctt ttttgaaagc agcaagtgct
     7381 tgttcactac cgtcaatacc tactaaaatg ttcttatatt cttgattcat cttgacccct
     7441 cctaacagat gtcagccttt tattacactt tctaggaacg attcgatttc ctgttttgtt
     7501 ttacgatctt tattcactag acgacctaat tcttttccgt cttggatcac gacaaaactt
     7561 gggataccaa aaatgttcca ttcagcggct acatctatgt acttgtcacg gtctacttca
     7621 ataaattgcc aatcagaaaa gtcattttcg atttcaggca tttgcggttt gataaaacgg
     7681 cagtctccgc accagccggc agtaaagaaa aatacacttt ttccagtttc tacgtagcct
     7741 gctaattctt ctaaactatt tgggataatc attctttttc ctctactttc tgtttatgta
     7801 tttttcaatt aaattataca cctttctttc gaactaacaa acattgatcc tctttttata
     7861 aaaaaaatct gacaaaaggt tgcttttgcc agattttata gtttcactat cgcgttattt
     7921 cccgtatacg atcgtgttca ctgttgattt atctaaacct ttcaatgttt gaatcaacat
     7981 ttctcgtgca gcttcaaaat cgcgaatact gaacatcgtt tgatgcgtat ggatataacg
     8041 tcccactacg ccaatcactg tacttgggat tccttcattt tgtgtatgag cagcacctgc
     8101 atctgtaccg ccttttgaaa caaagtattg ataagggatg ttgtttgttt cagcgatatc
     8161 tagcaagtac tcacgcaaac gaggaagcat gatcaaacct ggatcttgga tacgcatcaa
     8221 tgtaccttct cctaaatgtc cgaatgtgcc atttttcgtc actgtgtcat ctgctgcaga
     8281 gcagtctact gcaaagaaca gatctggctt gaatttattg acagaagctt tagaaccacg
     8341 caagcctact tcttcttgga cattcgcgcc tgcaatcaat gtatgaccta atgtttcatt
     8401 ttgtaacgct tctaaagctt ctaataccat tgtacagcca tagcggttgt cccacgattt
     8461 gctgatgata ttcttcccgt tggctgtttt gatcgtttca gcgtaaggaa cgattgtgtc
     8521 tcctggaaga acaccgtaac tcattgcctc ttctcttgat tcaaacccag catcgaacaa
     8581 aatatctgtt acttctactg atttttgtcc gcttgttcct cgtaataaat gtgggggaac
     8641 ggaagaagaa atacatggat aattcttacc agtagaggtt ttaagtgtaa aacgttgagc
     8701 agaaacaacg taagggttcc atccgcctag aggtacgact ttaaaaagcc cgttttcttg
     8761 gatttgtgtc agcatgaatc cgacttcatc catatgtgct gcgaccatta cacgaggtgc
     8821 atccgcttct tcatggtggc gcagcccaaa aatgccgccc aaaccatcta actgtaactc
     8881 atctaccagc ggttccatat gactttccat ataggcgcga acgtcttgtt caaaaccgct
     8941 tgttccttgt aattctgtta actctttgat tcgttgaaat gttttttctt ccatatgcta
     9001 ttcgtctaaa tttcagacta aactcctctc agacttatat tgatctgcgg ataaaatatg
     9061 attttttctg cagaccgatc gtatttatta tagcttattt tttctggtag cggttattta
     9121 tttcaaagaa aatatgagag tgtggtaaaa taaacacaaa gagtagcaag tgagaagaag
     9181 gaggtatatc cacatgtatg aagaaagcga attaagctat tttaaaggcg ggttagccgt
     9241 tgggatcggt ctaggattat tgagtggggt agcttcaacg atttggtatc atcgcaaaaa
     9301 agtgttatca gcagaccaag tgcttgaaaa tatcaaaaca tcttttttaa aagaaggcgc
     9361 aatcgaaggt gcttggatct cttttgaaaa agaacctatt cggaaattcg ccgttcggtc
     9421 gagtgcttat cggggtgggc tttcccggat agaagatggc gaaactgttt attatgaatt
     9481 tttagcagat gctcatacag gaaccgtatt agacatttca agaaaaaagg gcgcaataga
     9541 ttaatacgtc aaaaaaatga aataacgaat acgcacccac tatctaaaag agaggtatcc
     9601 tatccttctt ttatagtggg tgatttttat aaaaataggg ctatttcctc ttggaatagc
     9661 tagtatttgt agttattata cggtattctt gaaataagat tagttttatt atataaggag
     9721 agtgacaaat ggaatcacgt gaaaaaattg ccgtgctcat tccttgttat aacgaagagg
     9781 cgacaatttc aactgttatt gaagatttta aaagggaatt accagaagca gatatttatg
     9841 tctatgataa taattcgacc gatcgaacat acgaattggc cgttcaaggc ggagcaatcg
     9901 taaagaaaga accacgtcaa ggaaaaggga atgtaatccg tcaaatgttt tttgatattg
     9961 atgccgatta ttatttgatg gtagatggtg atgatactta tcctgcagaa gctgcacatg
    10021 gtttgcttga gaaattacgt tctggcgaag cagatatggt cattggcgat cgtctatcaa
    10081 atggaaccta ttttgatgaa aataagcgtc ctttccatga ttttgggaat aatctggtta
    10141 aaaatacaat tacttactta tataaaacaa aaattcgcga tgtcatgacc ggctatcgag
    10201 gattcaatcg aatttttgtc aaaagcttcc ctattatgag ttctggtttt caaatcgaaa
    10261 cagaattgac gattcatgct ttagataaaa aattcaagtt cgttgagatt ccaatcgatt
    10321 atcgcgatcg tcctgaaggt agcgaatcaa aattgaatac attctctgac ggattcaaag
    10381 tgatcatgat gatcatcaaa atgtgcaaag attataaacc tttgcttttc tttgggatct
    10441 ggaccatctt cttcttttta tttggattgg tcatggggat tcctgtgatt cgtgaatata
    10501 tgctgacaag ctttattaca cgcatccctt ctgcgatttt aagtactgga ttgatgactt
    10561 tagcccttct ttcattagta acagggttga ttttggatac ggtcgtcaca aatgccaaga
    10621 aagaatatga attgaattta taccatgtct accacgaata tcaaaataaa gaaatgaagt
    10681 aggaggcctt ttttatgcat aagaaaaagg agaatctatt tcctactttt tttgcaactt
    10741 tatttgcgtc aggttttgct ttttttactt cttatggtct aggaactttg aatccaacag
    10801 taatcattgg tttagccatg gttttgctac ttgcttttaa gcaatatgga atagagttgt
    10861 tttcaataaa aaacaaagtt gcagctattg cccgattgtt tttttctgca gcttttgctc
    10921 ttacactttc tattggcaga cagatacaat ttcagtatgt ctcagcaaac tatacaaaga
    10981 caacgattgc tgatggaagt attttttact ttcttgttta tttctccatt tttgtgtatt
    11041 cttttttatt tttacaatcg atttatttat caatgccaaa actggaaaca ttttttactg
    11101 ctcaaaacac acaggctttc tcttcaagaa aatatttttt attctttact gtctttttag
    11161 ttctgtgttg gcttccttat ctgttggcat tgtatcctgg agtggttttg cctgattcgt
    11221 taagttcggt cgcccaatct atgggtgatg ttcctataag caatcatcat ccagtcttat
    11281 tcaccttact ggtcaagcta tttgttcatg atttaccaac tgatacgatc aatcacggtg
    11341 tatttttatt ttcgattttt caaacattga taacagcttc agcgataagt tattctcttt
    11401 gctggctgtt aaaaagaaac atccattttt ttccggtact cattagttta tgctattttg
    11461 catttgcacc ggtttttcca atctacgcct tgaacatgca aaaagatact ttgttctcta
    11521 tcgcttgctt tttattttcc ttagcggttt ttgactttat ttttcaacga gaaaagccaa
    11581 cgattctcaa tcagatttac gtaatcatcc ttgctttact gaccacctat ctaagaaata
    11641 atgggattta tgtagtaacc ggcacaggtt tgatttgcgg cttgattatt ttgaaacaaa
    11701 aaaggtatca agcattcttt caagtggtgc tgggttatac agtgattgct ggactactca
    11761 tccacccgtt gatggctcgt tatgctattc ctacagaaac tgctgaacaa ttaggtgttc
    11821 ctttgcagca aatttctaga actattgtta tggatggtga cttgtctaag aaagataaag
    11881 cattcatgaa tcagctactc ccgataaaag attatgaagc gtattctcct agtcttgcag
    11941 actcaatcaa gtggcataaa aattttaaca atgagttttt gaatacacat aaaaaagaat
    12001 ttctatcact atgggcaaga aatttaccaa gtaatataaa aatctataca gatgcttata
    12061 ttttagaaac gttcgggttc tgggtacctg gtacaaaaaa tagttacggc tttttagata
    12121 ctcgtgtaaa cacgaataat tatggtatga agcaagtaga tttgattgaa catcttactg
    12181 gattgtcagg aatgaaaaaa atcatcgatg ctcgtgattt ctttggttcc ggcacattac
    12241 tgtggttagc tatcggcagt gcacttttag caatctttaa gaaaaaatat cggtatttac
    12301 tcctttttct tcccggtatg cttgtttttg ctactattat gattgctact cctgtggctt
    12361 ttagtttacg gtatgtgttt gttttagcat taggtctacc tgtttatatc ctctctcctt
    12421 ttttactaga taaaagagaa aaaatgaaaa cagatatagg agatactcat gaaactattt
    12481 aaaaagatat tttttgcttt tgttctcttg atcgctagct attgcgtact actgacaatt
    12541 acttttttca ttccgcagca ctctattgaa tcgaatgcag aaaaatctct acagatggtt
    12601 gaaaaagaag gaatgtatcc ttctatcaat caaggaaata tgctaggcac tcggttagat
    12661 aattttactg atcatttgat gatacgaaaa acaaaagcag atcctgaatt gaactccttg
    12721 gagaacgcga tgtctatggc ggattatccc cgttattggc atggttatca gcttttttta
    12781 cgacctttac tgctagggat gagtttgggg agtattcgaa tgatttacgc agccattctt
    12841 tttctgctga ttggattgac gagctacttt ttaaccaagt gtagcgacat ctatttaacg
    12901 acagccttgt tgatttcgct tgctatagga aatgctgcaa cctttttctt cagtatgcaa
    12961 ttttcaaata tttggatttt gactttattg tctgtattac tatttttaac aaaaccacaa
    13021 ctctccaaca ccaaccaaca attattcttg tatttctttg tcattggttc tttagctaac
    13081 ttttttgact tgttgacaac accggtcatc tcttggggga tacctgtgat cattatttat
    13141 tatgtaacaa ataagtattt aatggataaa cgcagttcat tgagcaaaca agtaggcagt
    13201 tttgttttta ccggtatctt ttggggatta ggctatggtt tgacatgggt ggctaaatgg
    13261 atcctatcat cgatcatatt gaacaaaaac attgtaaaag atgcaatcaa ccaaatcctc
    13321 tttcgaacgg aaggaaacga caaatacccg ttgcatcgat tagagatgct gaaaagcaat
    13381 attagattga tgtatccaaa agtagcaata ttattgctat tgattacttg tcttgttttt
    13441 ctctattttg cttatcgtaa acgaaatagc tggcgtccgt tccaacttct agttttgttt
    13501 gttttagctg ctgtaacgcc gtatatttgg tacaatattt tagctaatca ctcacagatt
    13561 cattattggt ttacgtatcg tacgcaaatc atcacaagtt ttgctatact tagtgctttc
    13621 gcctttttga tccctcctga gaaatcaaaa gacgaactaa attactttta agaataccga
    13681 tgtactaatt catttttaaa cataaaaact ccagctgtca gagatagccg gag
//
LOCUS       DANKEF010000066        13346 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000066 DANKEF010000000
VERSION     DANKEF010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13346
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.066"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(45..1364)
                     /locus_tag="PWZ48_002280"
     CDS             complement(45..1364)
                     /locus_tag="PWZ48_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2261921.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1361..2014)
                     /locus_tag="PWZ48_002281"
     CDS             complement(1361..2014)
                     /locus_tag="PWZ48_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2261922.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2302..2822
                     /locus_tag="PWZ48_002282"
                     /pseudo
     CDS             2302..2822
                     /locus_tag="PWZ48_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2724..3440)
                     /locus_tag="PWZ48_002283"
     CDS             complement(2724..3440)
                     /locus_tag="PWZ48_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HDL2261923.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3490..3699)
                     /locus_tag="PWZ48_002284"
     CDS             complement(3490..3699)
                     /locus_tag="PWZ48_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261924.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3976..5249
                     /locus_tag="PWZ48_002285"
                     /pseudo
     CDS             3976..5249
                     /locus_tag="PWZ48_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5356..6387)
                     /locus_tag="PWZ48_002286"
     CDS             complement(5356..6387)
                     /locus_tag="PWZ48_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2261925.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6394..7224)
                     /locus_tag="PWZ48_002287"
     CDS             complement(6394..7224)
                     /locus_tag="PWZ48_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2261926.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7221..8036)
                     /locus_tag="PWZ48_002288"
     CDS             complement(7221..8036)
                     /locus_tag="PWZ48_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDL2261927.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8033..8857)
                     /locus_tag="PWZ48_002289"
     CDS             complement(8033..8857)
                     /locus_tag="PWZ48_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HDL2261928.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSEKMQDIQELMRG"
     gene            complement(8847..9947)
                     /locus_tag="PWZ48_002290"
     CDS             complement(8847..9947)
                     /locus_tag="PWZ48_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2261929.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10125..10910
                     /locus_tag="PWZ48_002291"
     CDS             10125..10910
                     /locus_tag="PWZ48_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HDL2261930.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPYDLKLVSI
                     HQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGDILTHFDYGLRRFTFTAEE
                     FAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYRYAVPLYQSVGGKLFTLGS
                     DAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPLPIL"
     gene            10943..11326
                     /locus_tag="PWZ48_002292"
     CDS             10943..11326
                     /locus_tag="PWZ48_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDL2261931.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            11402..11533
                     /locus_tag="PWZ48_002293"
     CDS             11402..11533
                     /locus_tag="PWZ48_002293"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2261932.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            11502..11738
                     /locus_tag="PWZ48_002294"
     CDS             11502..11738
                     /locus_tag="PWZ48_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2261933.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(11816..12787)
                     /locus_tag="PWZ48_002295"
     CDS             complement(11816..12787)
                     /locus_tag="PWZ48_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDL2261934.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT         4056 a         2912 c         2305 g         4073 t
ORIGIN      
        1 ataagtctct atttttcaat aactattgca aaattatatc ttaattaaga aaatattttt
       61 attttaaaga taaaaaattc ttcatcctgc aatactttat attctatatt gtaattattc
      121 agaatattac ttacaatata taaacctaat ccattactat tttccttatt taaatcaaat
      181 ttaacatcaa atattttatc catattcgaa attttattgt taccgtatga attctctata
      241 tataaccaat cattaactat cccaatatta attaccccat ttacatcagt atattttacc
      301 gcattactaa tcaaattaga tagaataatc tttaaagctg tttttcctat ataaacattc
      361 tcgtctaata tataattatt gacagttatt ttcttttgat ttgctaatat ttcatacttt
      421 tttaatatgt cttctaatgt atcatttatt ttcaaatatt cttcatcatt attcaaattt
      481 tctatagaat gaactgataa tatttgagaa atattttttg ttaaactatc aacgatttca
      541 atacattcat taatataaat atctctttct ttatattttc caatattgta tttcatgttt
      601 tccagtatta ttttaagact agcaagaggg gttttaagtt catgggatgc acctttaaaa
      661 aagtcatatt ttaacttttc taattttaaa atttctttat ttttaaattc caaatcgtca
      721 attgttctta ataaagtaga atacaaatca ttaatttgtt gttttaattc tccaacttca
      781 tcattagagc taacttttaa actcattttt ttatcaagtt tcatcatttt atcagtaaca
      841 atttttattt cttgtatatt attttttatt gattttgcat aaattaaaga aataatagca
      901 gaaaataaaa ttgatattaa taaagaatat ggtaaaaatt taaggcttaa atctttcgca
      961 tctttttgca tatcagctgt agaaacaaat tgtagatttg tttttttacc atcattaagc
     1021 tttatttctc tttcttcaat tatcaaagaa ttactatcac tttctaaatt aacatttata
     1081 ttatctttga tttgtatttc attcttatta tttttttctt ttatgaatac ttttatttca
     1141 ctactcttgg aataaaactc caaagtttgt tctatatatt ttatttcttt tccattcata
     1201 ttactagaaa tttcatttgc catattatga atcttttctt ttctcgtctc caaatatgtt
     1261 ttaggaaaga taaaaaaaat taatgaatga actaatataa ttattattcc aagaacagaa
     1321 aatatttgta tgaacatttt tggaaatatc tttaattttt tcattttctc tccaatttat
     1381 atcctacatt tcttatagta gttatacagt ctaattgtaa tttttttcta agttctttta
     1441 tatatacatc tattactcga tcataaggag tttcttcgct atctttccat acataatcaa
     1501 taatttgcat tcttgttaac acttgtccat tattatccaa taaacatttt aatacttcta
     1561 gttcttttgc atttacatct atttcttcac catttatttt agctgtatag ctattaaaat
     1621 taaccgatag atccttatat tcaaacttct ctaaatgtcc ataattcttc ttaattaaag
     1681 aatctattct agcttttaag accggcaatg aaaatggctt ttctacatat ccgtctacta
     1741 aattagtaaa tgcatcaatt ttatattctt catcactaaa tgcagtcaag attagaattg
     1801 gtaaattgct tttctttcta atttctttca acacttctaa accatttata aaaggtatct
     1861 gaatgtctaa gataaccaaa tttatatcgc tattaaattt tgacaaggct tctcttccat
     1921 ctttagcttg aataacagta tatccaaatt ctgaaagata ttcacttatt ccctctctta
     1981 tcatattatc atcttcaaca gttaatattt tcatatatac ctccacttaa gcctatacac
     2041 tagatattat acagcatatt atggctctat aatatctagt gttggtgacg ccttctgttg
     2101 ccaatacttt ttcttttttt taataaatgc aaacagacac cttgaaatcc tttagaaaaa
     2161 aggcttctat aatattttgt gttgttggaa aatcgaaggc gattttctac taacactttt
     2221 tcttcgtttc aactcaaaaa gagactcact tttggtaaaa tttagtcgac caaaacatca
     2281 ccctaccaaa ggagtcgctc tatggataat catactagaa aattactcgg attaacagat
     2341 aaacatttat tttttgatga agaatggctg attgaaaaag aatataaagg cgttcaagcg
     2401 cagtttatta aagggagatt ggatgactca ccttcccatt gtgaacactg tggatcgatt
     2461 cgtattatcc gcaacggttc ctatacgaca cgtactcaga tgttgaaagt caaagaaaag
     2521 ttgactattt tagaattaaa acgtacccgt tttctatgct atgattgtgg gcagaccttt
     2581 tctgcgaaaa cggacttagt tgatgaacat catcaactaa caaaggaact gaaacaggcg
     2641 attctcatag aactgtacga aaatcaatca cgtaatctga aaatcagaag aagtaccgtc
     2701 ggctcaagcg atattggaaa ctcttactca aagattccac gacattggag ccgctcaaac
     2761 ggcattatca tcgactgttt aaacgaccaa ttagtcaaac agaaatcgtc gatgaaccgt
     2821 tgtgcacaat ggtgtcctga acgtacagcg acaccttcca tatccagagc agtcgccaca
     2881 tcacggggat ggatgccatc caaattaaaa gcaaccacgc cagtatgatg ttcaggatct
     2941 tgcggtccgt aaatggtgat accgccgatc gcttgtaatt taggtaaaac gtaggcaacc
     3001 agttcttgct cataacgatg aatattctcc ataccaatct cattcaagta atcgattgct
     3061 cctgcaatgt tcggagtacc agcttcaaat ttccatggca gctctttcca tgaactatcg
     3121 taaagattga caaaatctat catttcaccg ccgaattcga ctggctccat ttgttctaac
     3181 aattgacgtt tgccatataa tacaccgatt cctgtcggcc cgcacatttt atgcccgcta
     3241 aaggcgaaaa aatctgcatc caacgcttgt acatcaactg tcatatgcgg tgctgcttgg
     3301 gcaccgactg gactatcctc aactacaaca gcctgttcaa ccctcagccg tttcaagcac
     3361 aacaaataac cttctggatc tggtttgccg acctgaagat catcgatcgt cacaaaggaa
     3421 tcaatttttt ccttaatcat cggatgtgct tcaaaaaaat agtctagctc ccgcagttca
     3481 gaattgagac tactccgatt ttccagtgct gctgatccgc tgctcaaaat gttcagcatc
     3541 cactggtctc ccaaccgctt gacttatccc ttcataaaag gacagttctg ctgccgtccc
     3601 aacaccttgt tgatagtctg caaaaggaat tttatagccg aactgctcca gtaaataggc
     3661 ttcagtaaat agtgatgaat actctcgtaa aacatccatt cactgtcaat catcgtccca
     3721 tcaaaatcaa aaataagtgc tttggtcatc ttctttcccc cttacccaaa acaagcaaaa
     3781 gctgtgtcct gctctctcta cagtatcagc ataaaaactg ccgtctgaaa agtcaaaggt
     3841 gaaagaaatg tggcgctata atttctagga ctgatagtaa atcattctat cagtcctttt
     3901 gagcataaaa aaacaaaaca tacagaagct ccgttagaat tgaatcacca caacacaatc
     3961 aaaggagcgt tctgtatgcc tgatcttcat cttatcaaac tattactagg aatcaaagat
     4021 ggaaacattc aaatcactca tgtcgagaag aaaaaaatac attcaatcaa cagtatcgtt
     4081 gtttctgcca cgctcattaa aacgattcag ccctgtcctc agtgtaaaca ggtgaatcaa
     4141 catgggctga tcgttaagaa cggaaagaaa aaatcgctgg tccttctcaa taagtgtgcg
     4201 aatcaacgga cttatcttgc tttggcaaag caacggtacc tctgtcgtgg ctgtcacacc
     4261 tattttacgg cgaataccta cattgttgaa aaaaactgtt ttatcgccaa acaagtacat
     4321 tataaaatat tggaggaatt gactgaaaaa caagcaatga cgacgatttc taaacattgc
     4381 ggcgtttctt ggtcaaccgt ttcgcgaaca ctcgcttctc tgctccccat gacaaacgtg
     4441 aaaataaatt ggttgcctcg ctgttcattg gtagatgaat ttcgttcatt gaaaaatcaa
     4501 gtcggtccct attctttttc ttgtatggat ggcgatactg ggaaattact ggatattctt
     4561 ccttcacgta agaagaaaga tctcatttct tattttatgc aatttgaacg acgtgcacgt
     4621 ttaaacgtaa aaatactcgt aacagatatg aacgcctctt atgctttgct gataaaagag
     4681 tgctttcctc atgccaaact cgttgtggat cgctttcaca tcgtcaagca cctcattcgc
     4741 agttttaagg atatttgact atgagtcatg aaaagcttcg accgaaacga tccaatacaa
     4801 gccaaacact atcgacaagt gaaggcttta tctcgacttc tgatcaaacg acaggacacg
     4861 cttgtttatg ataaatggac aaaatggcgt aattttggtt gggcctattt aaccgaaagt
     4921 gaagtcgtag aacgtctact ttctacttct gatgaactac gaatagctta tgcttactat
     4981 caagaaattc ttcaagcttt ttatgacaaa gaagcggaca tcttctttca gcttgtaaaa
     5041 acaatgccca agtcggttcc aagagaactg catcatataa aaaaagcgtt tataaactat
     5101 gaatccggca ttcgtttagc cttagagttg ccttactcaa atggcaaaat agaaaatctg
     5161 catacccaca tcaaagcatt gaaacgaata gcttacgggt taggatgaga accagaattt
     5221 tcttaatcaa tcatctgatc acttattaaa cataaaaaaa catctaaagc agaaaatcaa
     5281 ttctacctta gatgttctta tatcactcta tcagtcctat ttgacaaaga gccagaaatg
     5341 tttgccgact atacactagc tttgttgtgc aaaatgctga gtatcaattt ccacagggat
     5401 tgctagaata tctttcagct gaatgtataa ggcatcgcta attttctctt cccgatcgtt
     5461 caaccgatcc actaaaattt ctttgccctc cagcgtttga aaaacatacc gttgaatgtt
     5521 gcctaaaata aatgattcct tccatagaac cggaatctta accccctgag gaaccggttc
     5581 aaattgaatc agctccggtc ttaaataata aatgcagcga ttgcccttag cggaaaactc
     5641 gtgggtatac tgattcagct cataccattc gaaacgatta tagttgccga taaattccgc
     5701 aacaaatcgg ctctgaggat tctgatacaa ttccttcgag gtggaaacct gagccacccg
     5761 cccagcttcc atcacaaaaa tccgatcact gattcgcatc gcctcttctt ggtcatgagt
     5821 cacaaaaatc atcgtcatcc ctagcgattg ctggatagcg cgcaaatccc gctgcagctg
     5881 ttttctaatt cgggcatcca aagcacttaa tggctcatcc agcagcagga ctttcggttc
     5941 agtgaccaat gagcgagcca gtgccacccg ttgtcgttga ccgccagaaa gaaactggac
     6001 atttttatct gcctgctccg tcatatccac caatgatagc atctcaaaaa ccttttcctg
     6061 aatcacctca ggactcagct tctttacctt caaaccaaag gctacatttt caaaaacagt
     6121 catagttgga aacaatgcat actgttgaaa aacaaagccg atattgcgat ccttcgtagg
     6181 cttatcggca atattctcat tgtccagcac aatctcaccg ctctttggtt gttccaatcc
     6241 cgtgatacag cgcagcaacg tagactttcc gcacccgctg ggacctagta aagtaaccag
     6301 ctcacccttt tcaatctgaa tttcatcaat agctaaaatc tgtttttcat caaaaaacag
     6361 attgagatca tgtatttcta aaaacatttt ccgtcacaac tcctttaagg gcttcttttt
     6421 cgactgcttc tcttccctcg tctgcttaag ggacagctgt tgcttttgtc gataagtcag
     6481 cataaaggta gttaaagcaa ttcccaccat aaacagcaaa tacaaaacca tcacagcgct
     6541 agccaaatgg ccgttttcgt tcatccgccg cagcatatag atgcgtaccg tctgaaatcg
     6601 accgccaatc aacaggttcg tcagcatgta ctccccaaaa accgaagaaa aaatcatcag
     6661 agaaactagg gtcacaccga tacggatgtt tggaaacagc acctggataa taatcgtaga
     6721 catcggggcg cccaaggttt ctgctgcatc cactaattcc ttcagattaa ttaagcgcat
     6781 ctggttgtta agactaagat agacaatcgg caacgccgta ataaacaatg cacctaccaa
     6841 cactacaaac attgggatac cagtttcagc atacgttcga agcaaagccg ttaccaaaat
     6901 cactcctggt aaagcatacg gcagcaggag cagcttttcc agccaacgat tcaaacgagg
     6961 gaaatacaaa tgaatccaaa taatggtggg aatcatcaac aacagaatcg tcaccaagac
     7021 cacacctgct aataaaagtg agcgacccac cgcagcaata aattccctgt ccgtcagcag
     7081 ctgttggaac cattgcaagg taaaatcact aggtaaaagc gattttgacc atttagtgga
     7141 gctggcatac aaaatcgttc caataatcgg cagcaagtaa tacaggacaa agcagaacaa
     7201 aatcggtttt attaactttt tcacgtctgc accaccttgc ctttcttttg aataatcaac
     7261 attgccgtta ccagcaggac acctaaagcg actgccaacg cactgcctaa attaggcttg
     7321 gcgaacacat ctcccgacac taaagccgca attcgaatcg ttaaaagatt tacattactg
     7381 ctggtcaggg catacgctgt ttcataggtc cccatgccat tagcaaataa gatgacaaag
     7441 gtgctggcaa tcgatggtcg caaaaaaggc aaagccacct tgaaccaagc atacgctgat
     7501 gaagcgccca gcaattgtgc ggactccagc cattcctttt tcacttcctt catgctggga
     7561 tacaaaaaca agacccctaa cggaatttgg aaataggtat acgtgatggt caatccccac
     7621 caagaataca aattgaagtc cgctagaaat ggcaaaatca ttttcagcac acctacattc
     7681 cccaacaaaa taataaaaga gaatgccagc ggaatcccag caaaattagc tgccaaattg
     7741 gaaacaaagg tgattttctc ctgcaattta gtcgatagat tcattaagca taaacataaa
     7801 ataaacgtcc cgattaaccc aatcacactg gagctgacag caatcagtac gctgttaaca
     7861 aaggcctgtg tgtaatattt gttgctgaaa atatcactgt aattggccaa tgtccactgg
     7921 ttagttaaat cactgtgcaa gctgcacaga accataaaaa gcagcggcag cagtaaaaat
     7981 gcacagaaga taaaggaggc aggaaccaga accaagcgtt ttttcatctc tttcatcccc
     8041 tcatcaattc ttgaatgtcc tgcattttct ctgacggtgc aattcctaat aaccagcaaa
     8101 acaatggagc cgccagcagc tgtggtacag tttgttcctt cagcttttgg cgtttatttg
     8161 aaatcaaata aaaaggaact tcacgatgtg cagccaagct gccgccatgc agtccgtgcg
     8221 catccatccc gtgatccgca gtcaccacga cttgataacc tttttgcagc cattcaggaa
     8281 ggtaaatagc caataatctg tccgcctgat taaccgctgc tgcatattca tgagaatcgg
     8341 cggtaaattt gtgaccgaca tcatcaatat tcatcgaatg tacgaccaaa aagtctggct
     8401 gatactgatt cactaaataa tgggcatccg caaataaatg cgaatcaggg taagtgtcct
     8461 cactataaaa gatgccatgc tgaatcagtc gctcctcctg atgctggatg cgatcaacca
     8521 aacgattata gggggcttga ttgtacaact cgctgtacca gtggtaagcg gcggctcctg
     8581 tttttccgcc agcttcctta actaattcaa acagcgacca ctctttggat cgctgagcat
     8641 ggtaattact atagatgcca ttttcatagg ctggaacgcc ggtcatcagt acttcgtata
     8701 acggacgtga attgctgggc agctcacttt ttaccttcaa taagcttgct tgctggtgct
     8761 caactaaatg attcatgaac ccaagctggg cgacggctgt gtcgtagcga cagccgtcta
     8821 gtataactac aatcagtttg tcaagtctac tttgcatagg tcagtacctc cgcctgccaa
     8881 agctctggaa gctctacaga ggtcttgttc caagcttcat tgtcttcaac tggcaccgcc
     8941 gcttcataag catcctccgg cagtaatttt tcagctactt ctttaggcag ctctaccttg
     9001 cgaataggtc gcgcatagcc ctttgccaaa ttgatttgcc cttcatcaga taaaatatat
     9061 tcccgtgcta gctttgccgc attgggatga ggggcatttt tattgattaa ggttgtatac
     9121 ccgctaatga tggagccgtc tgttggcaca gttacctcaa aacgctcgga gtcaatttgg
     9181 tcacgataat ttagtccatt gaagtcccaa acaactgcca catcgatttc acctttttca
     9241 agatttgcta cgctggcatc aacggtggac aaacggcctt tttctgccat ttttttgtag
     9301 taattcagac ctggctgaat atccttttcg tcacctccgt tggcaatcgc cgcagccaat
     9361 acagcaaatt gtgcttggtt ggcaatcgtc acatccccca cgctaacttg atagtcaccc
     9421 tcttgcagct ccttccagct agtcggtgct tgcttcacat tctctttatc ggtgataaag
     9481 gaaatggttc ccgtatagct taacaaccaa tccccatcat catcctttgc ccaatccgga
     9541 atttcctccc aatagttggt cttataaggt aaggttaact ctttgctttc agccaacgga
     9601 ccgaagctga taccgacatc accaatatcg gcggttccat tttttccttc tgattcaaac
     9661 ttagccaatt cttctgcact ggacatatcc gtatccacgt gacttaagcc gtattctttt
     9721 tcaaggtcgt tccaagtgcc tacccaattt gcccaagtat ccggcattcc caagctggca
     9781 atctctcctt cttccttggc tttttctata atcgtatcta aagagtcgtc tgccgcagcc
     9841 gattgcttcg ttgcttcacc tgagccgcag cctgtcagca ggatacttac tgttgctgca
     9901 caaaataaac cgatagtcat gttacgaatt ttttcattca ttttcattca ttcttctccc
     9961 ctttcgataa ttccattcta acaaagcttt gtaatgagag aatcagtttc ttgaaaaggt
    10021 ttcatgaaca atgtaaaggt tgtatatggt ttgtaaattt cgacgaattc ccgttaacct
    10081 tctaaagctt ttgcggtatg atagtagaaa aaaggaggct ccctatgcag tattatgatc
    10141 agcatctcca cacgtatttt tcctttgatt cagaggaaaa atttgaaaac tatttagcct
    10201 atcagccgga gtttttcgtt tctacggatc actttgattt aaacaatcca tctaccggaa
    10261 aagatgacat tccggattat ccagcctata taaacaaact ggactcttta aaagaaacct
    10321 attccaccaa atttctgcga ggtatcgaga ttggcgttgt tccaggacaa gaagagcgga
    10381 tcgtggatta cctcgctcag catccttatg atttaaagct ggtaagtatt catcaaaatg
    10441 gccgctttga ttatatggac gatgtggttt taaccaaaga tcccattcaa actaccaagg
    10501 aatattttga tcagatgttg caggtcttga cggattttcc agaaggagat attttgactc
    10561 actttgatta tggattacgc cgcttcacct tcacagcgga agaattcgcg gagcattttg
    10621 aggctcagct gctaacgatt ttcaaaaagg ttgttgaacg agaattggcg atggagttaa
    10681 atgccaagag cttcttgaaa tacggcaacg aagaccttta ccgttatgcc gttcctttgt
    10741 atcaaagtgt cggcggaaag ctctttacct taggctcgga tgctcatgtt gccgaagatt
    10801 atcagctggg ctttaaaaaa atgagtcagc tgttggcaga ctgtcaggta gaaaagttgc
    10861 tagtcattca aggaaaagaa cgccgcctga cgccgctgcc gattttgtga ttcctatata
    10921 atagaagaaa gtggtgaaaa aaatgagcaa cctgtgggac tacaatcaag aggcacccat
    10981 ccattattta attgcgcgac attgggatgc gttgaaaatt gaggcggttt gccgatcctt
    11041 gttagcagcc gttccaaaac aacaactgga aaattttttg gtggctgaca gcttgcagcg
    11101 ggaaaaagtt caggcttatt ttgcagcctt taaagatcag cctttggaat atttgcatgc
    11161 gcagtttcat cttttttatc aagtagccgc cccagatgac tacaacgatt tacgaggaca
    11221 gctgcaattg acctttcaag cggatgaaac cgcctatacc gttttattgg gaatggctcg
    11281 ccttggtgat caggcgaagg tcgagtggcg gattttcgat atttgatggc aggatcaagg
    11341 cgtttcagtt gcaaaccttc ctctctttca attagaaaaa ccgcagcagg aagaagtttg
    11401 tgtgctggtc accgatgctg gtgaaattcg tctccgctta tttcctgacc aagctcccct
    11461 ggctgtagcc aactggcgct ccctagctga gcaaggcgtt tatgacaaca cacctttcgg
    11521 ctcgtgtcat taaggaattt gttattcaag gtggcgcttt ggatggttct ggtgatgaag
    11581 ctgtttccag ctatgagggc tttttcgctg atgaagtgca tcgcggactt tatcatttta
    11641 atggcgcctt ggccttaggg gaccacggcc cccacaccaa cggcaaccag ttttttattg
    11701 tgcaaaacac aaaggcgcag gctgacctgc taatgtaaag caagcatact tggagcttgg
    11761 aggactaccg gagctggatg gtcgttatac tgtttttgga caggtatacc aagggttaga
    11821 aatcgtagca gcgatcgcag ctcagccgac aaacagtgaa gatgtaccga ttgagccagt
    11881 gttcattcga caaattaact tcgaaaaaac atcgtaaaaa gacactcact gacttcagca
    11941 aaagctctat tcttgagctg gctgcagcta gtgagtgtct ttcatttatc cataagataa
    12001 agcttggatt caataaataa ataaatgttt tggttaaagc tgtgaccagc aaatgaggaa
    12061 ccgtgcgagc cagttgatct gccaatgcaa acatttcttt aggtgaaggc gctggcgtgt
    12121 ctgccaacgg atactccagt cccaatgctt ggtaaccgtg cataccatag cagtggtacg
    12181 gtagtaattc taacggtacg aaccgatcaa gatctgccac aaaacaagca atcgtcagca
    12241 gctcggtcag gttcatattg atacctggaa ccacaggaac tcgaattcgc acttcgcagt
    12301 taggatactc aattaaaaag cgacgaatat tcttcaaaat ttgttggtta tctaagccgg
    12361 tccattgttt gtggagcttg ctggtaaaca tttttacatc cacaaacaat gtatccacat
    12421 agggagctac cttttggatt tcattataag caccacaaaa gctggtttcc agtacggtat
    12481 tgattccaat gtatttcgat ttttgcaaaa tttctttagc aaaatccgcc tgaaccagtg
    12541 cctcgcctcc gctaatggtc actccgccat ccgagtgaaa gtaaaaaaca gcatccttct
    12601 caatctcatc cattacctct tcagcggtca tggtctggcc aaatcctttt tcaatctgct
    12661 tgctttgtga ttcgggaatc gaacaccagg cacagcgtaa tgggcagccc tttaaataaa
    12721 caacggtccg taaaccagca ccatcatgta gagaaccctg ttctattctt aatactgttc
    12781 cttccatata gatttttgaa ttacacacta gtttaaacta aaacacacaa ggaaaaactt
    12841 accatttaat cataaaatta gctcatataa atatactagg atgtaaaata gactggtcaa
    12901 aatctggtca aaaaagagga ctaatacagg tagtcctctt ttttgttatt ttaatattct
    12961 aaatcatttt ttattggttt attcccttat tcgatgtaga ctcaaatcac tatatcacta
    13021 gatatcaaac ggcgtcatac ggaatcaaaa acatcaaatt tgacctgtta aatggtcatt
    13081 gaataacatt caaaatcaat aaaacggagt cagacacgga gtcaaaaacg ctgagagtaa
    13141 aaaatatata cagccttgta ggtaaagtac cacagggctg tatatttttt atttcagatt
    13201 accttaaatg attccattta ctttttatct cattttttcc tactagttag tatcaaatac
    13261 tctcctattt tctcaataaa ctatttttgg atacatggta tctgaaacag ctaaaacctt
    13321 gtcattacag caaaaaatca cgttat
//
LOCUS       DANKEF010000067        13302 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000067 DANKEF010000000
VERSION     DANKEF010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13302
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.067"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            60..2849
                     /locus_tag="PWZ48_002296"
     CDS             60..2849
                     /locus_tag="PWZ48_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDL2261935.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2896..3423
                     /locus_tag="PWZ48_002297"
     CDS             2896..3423
                     /locus_tag="PWZ48_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HDL2261936.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3444..4634
                     /locus_tag="PWZ48_002298"
     CDS             3444..4634
                     /locus_tag="PWZ48_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2261937.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4674..5972
                     /gene="asnS"
                     /locus_tag="PWZ48_002299"
     CDS             4674..5972
                     /gene="asnS"
                     /locus_tag="PWZ48_002299"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDL2261938.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7148..7822
                     /locus_tag="PWZ48_002300"
     CDS             7148..7822
                     /locus_tag="PWZ48_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HDL2261939.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            7819..8160
                     /locus_tag="PWZ48_002301"
     CDS             7819..8160
                     /locus_tag="PWZ48_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261940.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8190..8549
                     /locus_tag="PWZ48_002302"
     CDS             8190..8549
                     /locus_tag="PWZ48_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDL2261941.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9074..11158
                     /gene="cadA"
                     /locus_tag="PWZ48_002303"
     CDS             9074..11158
                     /gene="cadA"
                     /locus_tag="PWZ48_002303"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HDL2261942.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            11449..12915
                     /locus_tag="PWZ48_002304"
     CDS             11449..12915
                     /locus_tag="PWZ48_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2261943.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4403 a         2311 c         2876 g         3712 t
ORIGIN      
        1 tctgcacttt gtagtataat ttcaaaagaa cattagaggc aggagaagag gaagatattt
       61 tgaaaccaag aacttatgca gtcgttgata tcgaaacgac tgggacgaat ccaaaagaag
      121 atcggatcat ccaatttggc tgcgtcatga ttgaatcagg tcggatcact tcacgttttt
      181 cgatcgatat tcatccaggg cggaagattt caaaacaaat ccagcattta acagggatca
      241 cgaatcaacg cgtccaaaaa gcgccttatt tcgaagatgt cgcccaaacg atttacaatc
      301 ttttggcaga caccatattt gtcgcgcata atatttattt tgattataat tttttgaacc
      361 atgaactcat gcgatgcgga cttcctagtt tgaagatacc aggcatcgat acagtagaac
      421 ttgcacaaat tttcttgccg acggaaccaa gctttcgctt agctgatttg tcagaaagcc
      481 ttggatttat tcatgagaat cctcatcagg cagatagtga tgccgaagtg acaggtcaac
      541 ttcttttgct gatcgaagaa cggatgagaa aattgccaat catcacgtta gagcagattg
      601 cgcgattaag tagacacaca ggtatggata cgagtcgttt catttatcat gtgattgaag
      661 aaatgaaaga aaaaccagag ccacttgatc cctcattaga aatcgtcgat gggctagctt
      721 tgcagaaaaa agaagtggaa ctattcacat ccgtccatta tggcgagcgg acatatccaa
      781 gaaaaaaaca agcaaaagaa aaaatgtttg gtaaaacact gatgtatcga aaagaacaga
      841 acagactgat gaatgcggtc tacgaccatt tcacaaaaga tgaatcaaaa gatttgatga
      901 tcgaagcagc tacaggaatg ggtaaaacaa tcggttatct gctgccattg agttatttgg
      961 ctacacctga aaaacccgct gtcatcagta ccgtttcgct agttttgcag cagcagatca
     1021 tagaaaaaga cattccattg ctgaatcaat tgctagatca gcccatacaa cctgtaatta
     1081 tcaaaagcta tcgccactat attgatttgc agcgttttaa aggcacactt gtggaaccgc
     1141 cagaacaaaa acaatatgct ttgtatcaaa tggcggtctt agtttggctg acgcaaacga
     1201 agactggtga tttggatgaa ttacatttaa caaatttgaa ccattcattc tttgcagata
     1261 tcgcgcacag aggtattgga tttttagcaa gaaatcagtc attttacgaa caagactttg
     1321 ttcgtttctt gcaaaagaaa atcaggcaaa gtaatttttt gattgtcaat catgcgtttt
     1381 tagtacaaga aacacagcga aaagaaccat tgcttccagc tagtccattt ttgttgatcg
     1441 atgaagtaca tcatttgccg gaaatcgctg aaaaagtaaa tgacaaacga ttaagtatta
     1501 actatttcca taaacaaatc cagcaattag aagaaccaaa tcagctgttt gaccgaatac
     1561 aaagctattt accgaaagaa catgaagcct tacgctggtt atcgatttat cgagaagaac
     1621 tttctgcgct atgcgaagca cagatttgta tggcagaagg attaatgagt ttagtgagcg
     1681 gcaagcaacc taacggacag tatccagaag aagtcatgat gacaaaagag atgttggatc
     1741 agctgccact ggaatctggg acagccattg atcacctgca gcttttttat caggagatcc
     1801 agcagctaca gtcacaaatg cgtctgactt ttgagattat ccaagaaagc tggacaaacc
     1861 agatgcggat cgatttagca agtttatata gcctatttga tcaattagac aagcaatact
     1921 tgctgatgga tcaatggctg gaagactggc gagatcattt gatccattgg ttcgttcctg
     1981 ggaacacagc gcagcagatg atttttcatc tccatgattt tcaagcggct atgttgccgt
     2041 cgactgtttg gtatcctcga tatgatcgta ttctttatat tggaggaacg ctgaagatcg
     2101 gccctaatcg acactatctc tctaagaggc ttggcattga agcagcacct cttaaagtga
     2161 ttgcctctcc ttatgattat gagaaacagg caaggatcta tgtgccaaaa gaggcacctg
     2221 ccgtccatca agtcagcaat caagcatata tcgattattt agagacagca cttacaggtc
     2281 tgatcgaaca agaagatcgg ccaatacttg tgttattcac ttctcatgat gttttgcaga
     2341 aggtctatca gaaaatgcat ctgtctatgc tagaaaaagg acgagaaata ctagctcaag
     2401 ggtttggtgg aagccgggag aagcttttga aacgcttcat tttgtcagaa aaaagcgtat
     2461 tgtttggcgc tgacagcttt tgggaaggtg tagatttgcc tggagatgct ttgcagatcg
     2521 ttgtagtcac acgtttaccc ttcgataatc cgcttcgccc gatggtcaaa gctaggaacg
     2581 cttatttaga atcagagggg atcaatcctt tttaccagga atccgtacct aaagcagctt
     2641 tgaagttacg ccaagcatta ggtcgcttga taaggacgga gacagatcgt ggcgtattgc
     2701 ttattttgga tcgaagacta gtcactgcaa aatatggcgc acggatcatc catgcattac
     2761 cgaaaaaagt accgatcaaa gagctagcta tggaggaaat ctatcaggat atccatgaat
     2821 tcttaaagaa aaatgaagaa ggaagttaga ggagctgtct tttttcataa ttaaaatctg
     2881 ctataatgaa aaggaatgaa gataaaggag cgacgaccct tgaagaaaaa acagacaaag
     2941 gaaaaaacga ttaatcgcgt gttacttggc ttgatcgtat ttttattagc ggtcatcgtg
     3001 atcagctcga ttttttactt gcgttccagt cgtccggcgg tgcaggcgaa gaaagaagca
     3061 gtagaaatcg caaagaaata tgcaaatctt gaggaaaccg atcattttta ttggtttact
     3121 cgaaagaaga catacttttc actgacaggt acaaatgata aaggaaaaga aatcgccgtt
     3181 atcattccta aatctggtga aaaagtaaaa gtattggatc aaacaaaggg actgactgaa
     3241 aacgaagcaa aacagacaat tgctgcggcc catcctgaaa tccaaatcga aaaagctgct
     3301 ttagggatgt atgatgatca gcctgtgtgg gaagtagtag gaaaagaaac aaatgggaaa
     3361 ctgaattatt acctgattgg ctttgaatct ggagatgaag tcaagacaat caagggaatt
     3421 taaaaggtaa aggaatgaat gggatggagt tatcaaaaag aacaaaacga ttagaaccgt
     3481 ctgtcacttt ggcagctgcg gcaaaggcca aggcattaaa agcaaaaggc agagatgtgc
     3541 tgagtttgac tgtgggagaa ccggattttg cgacaccaga aaacatccaa gaagcagcaa
     3601 tcgaagcaat ccgcaatggg aaagctagct attatacacc aacagcagga atcccagagc
     3661 ttcgacaggc aatcgttgat tatctgaaga aaaactatgg acttgtctat gaaccttcac
     3721 aaacagtcgt aacagacgga gcgaaatttg ctttgtatac ccttttccaa acgatccttg
     3781 acccgcaaga tgaagtcatc attcctgtgc cttactgggt cagttacggt gagcaagtaa
     3841 aactggcaga aggacttcct gtctttgtga aaggggaaga atccaatagc tggaaagtta
     3901 ctgttgaaca gctagaacaa gcacgttcag aaaaaacaaa agccgtaatc atcaattctc
     3961 catcgaatcc gactggaatg atttatagca agaatgaact gcaggcaatc ggtgaatggg
     4021 cagtaaaaaa tgatattttg atagttgctg atgatattta tggccgttta gtttataatg
     4081 gcaatgagtt tactccaatt gcaacgattt cagaagcaat caaaaatcag accatcatca
     4141 tcaacggcgt gtctaaaaca tatgcgatga caggctggcg cattggttat gcggtgggaa
     4201 atcctgtgat catcaatgga atgatcgctg ttgcttccca atcaactagc aatccgacag
     4261 cagtcagtca atatgcagct gttgaagcat tgaaaggtga gcaagataca gtagaagaaa
     4321 tgcggaacgc ctttgaagaa cgcctgaata cgttatatcc gcttgtggca gaactgccgg
     4381 gtgtttctct tgaaaagccg caaggagctt tctatctttt tcctaatgta aaagaaacgc
     4441 tacgcatgtg taagtatgaa aatgtcacac aatgggtaga agatctttta gaagaaacag
     4501 gggtagcgct tgtgactggc gaaggctttg gggcaccaga aaatgtccgc atgagttatg
     4561 ccactgatcg cgctacgtta gaagaagcgg tacgcagaat caagcaattt attgagagta
     4621 aaagtcaaaa ttaatcctga agaaattcaa ggaatataaa aggagagtca tacgtggaac
     4681 aaattcaaat catcgattcg aaaaaccatg tgggagaaac agtaacaatc ggcgcttggg
     4741 tcgcaaataa gcgatcaagc ggaaaaatcg catttttaca attaagagac ggaacagcct
     4801 ttttccaagg tgtagttgta aaaagcgaag tgtcggaaga agttttccaa acagctaaaa
     4861 atctacacca agaaacatcc gtgttagtaa ctggagaaat ccgtgaagac agtcgttcaa
     4921 aatttggtta tgaaatcgga attacaaatg tggaagtggt aagcgagagc cacgattatc
     4981 cgatcacacc aaaagaacac ggaacagatt tcttgatgga tcatcgtcat ttatggctgc
     5041 gttcttcaag acaacatgcg attatgcaga tcagaaatga aatcattcgt gcaacgtatg
     5101 aattctttaa tcaacatcat tttgtcaaaa tcgatccgcc gatcttgact ggttctgcac
     5161 cagaaggcac tacggatcta ttcgaaacaa attactttga tcaaaaagct tatttatctc
     5221 aaagtggcca attatacatg gaagctgctg ctatggcttt cggaaaagtc ttttcattcg
     5281 gtccgacttt ccgtgctgaa aaatcaaaaa cacgacgtca tttgatcgaa ttctggatga
     5341 tcgaaccaga aatggctttc actcatcaag aagaaagttt agaagtacag gaacaatatg
     5401 tagcattctt agtacaaaat gttttagatc attgcgaata tgcattagat gtactaggac
     5461 gagacaaaga agtcttaaaa acttatacaa aattgccatt ccctcgtatt acttacgatg
     5521 atgcaatcga attactgaag aaaaatggat ttgatgacat cgaatggggc gaagactttg
     5581 gttcacctca tgaaacgttc attgcgaatt cattcgaaca gcctgtcttt atcctaaact
     5641 atccaaaagc catcaagcca ttctacatga aaccacatcc aacaagagaa gacgtggtaa
     5701 tttgtgcaga tatgatcgca ccagaaggtt acggtgaaat tattggcgga agcgaacggg
     5761 caacagacta tgatttcttg ctagaacaaa tccgtaatca cggtttggat gaaaaagaat
     5821 attcatggta tcttgatcta cgtaaatacg gttctgttcc gcactctggt tttggtctag
     5881 gccttgaacg aacagtcact tggattgctg gtatcgacca tgtacgtgaa gctattccat
     5941 tcccacgttt gttgaaccga atttatccat aagaggtgat gaaaaagctg ttctgcatca
     6001 agtaattaga acagccaagc aaaaacagct gttcatgttt ttgaattggc gggacttatt
     6061 accgcagatg taagcttttg aataccgttt agtggagagg ttgtgaaaaa gctgtttagt
     6121 ttcgagtatt aagaaaaatt ttgtggaaat ggctttccac atttttgcat aaatttggct
     6181 taataccgag aaactggctt ttgaacaccg tttaaggaag aggttgtgaa atcagtgcct
     6241 tgacctgagt attagagaaa caaaacggaa aaatagcttc tcatatttta ccgttttttt
     6301 agctaatatc gagggtcagc tgattgaaca ccgtttatat caagaggttg tgcgcctgcc
     6361 gttcagctcc gagtcacaag gagtaatttc aaaaaacagt ttttcatgtt tgatgactat
     6421 tgtgacttgt gaccgaggag ttggccggag agcaacgttt atttaaaaaa aagaactgcg
     6481 aactctcgca gttctttttt taaaatacgg gataaaggaa gtttccagca gacaagagaa
     6541 gaaagcaaaa gaaattgcta tttgattcac aaggttctat aacgaaatcg caatcaatca
     6601 aacatataca aagaaggtta ttcaaatgga aatctgattt gatgatgctt ctgctgttga
     6661 aataaagcag catctagtac ctgaaaacaa actaatagta acattcgaag atggagtagg
     6721 tccgtatttt cagcatgcaa tgatccatat gtaagtttaa ttcatccttg aaatcatcgg
     6781 accagataaa gttatgacca atctatccca agcaatctag aagagatatt agtaaaagaa
     6841 catttactgg aagtcttaga tgagcctata aatatatgtt ctgatggcgt catcagtggt
     6901 ctgattgacg gaagcttagg tgtgatcgat ttctctggac caaacaacga aggaatcaag
     6961 gggaatccgg cacgttaaga aaaaaagtat aaaaaactag acacttgatg agcgttcatt
     7021 caattgattg aagggactag gctggtgtca ttttttatac atatattttc tctttcgtct
     7081 tgatagggct tatgggataa tataaggaag aaaatcatcg ttttattcaa aaaggagttt
     7141 ttgacaaatg aaaaaacgta tattatttat cggtgacagc attacagacg caggaagaga
     7201 ccgagcagaa gcaactgatc taggtaaagg ttatccactt ttgattgccg gaaaactaat
     7261 ctcccaatat ccaacgatga aatttgaatg catgaaccgt gggataggag gagacaaaat
     7321 tgcagatctg caagtacgct ggcaagaaga ttgtttagct ttacaacctg actatgtatc
     7381 gattttgatc ggaatcaatg atacctggca taatactagc gatcaggcta tattcggaac
     7441 tgagaaatct gcgcagcaat ttgaatccgt ttatcgaaaa ttactaaatg aacttcaaac
     7501 agcggaaatc tgcaacatca tcttgatgga gccgtttgta cttcctataa ccgacgaccg
     7561 tcatggctgg cgtaaagact tagaccctaa aatccaaatt gttcgtaaac ttgctaagga
     7621 atatggcgca ttgctggttc ctttagatgg catattcaat gctttaggga ttgcttatgg
     7681 tttttcgaaa tatacaagag aagatggggt ccacccaacg attggcggac atgagatcat
     7741 tgcacaaagc tggatagatg caattgccgc agtcgatttt tttagtggag ataaaaatga
     7801 gcgaaagaaa agtgagaaat gatggaaata cggtgggcag taaaagaaga tttacctcaa
     7861 atcaatcagc tgattactga tcatccagaa tcattcgtaa aagcaaaaac ggcagctgac
     7921 ctaaatgata agaataaact attgctcgtt gctgtaacaa aagaaaaacg gattatcgga
     7981 tttgcgcaag cggacgtgca acagtcagaa gaacgggtgg atcagcgacc atcaggaaaa
     8041 ctaatccaac acacattagt aacaaaagaa aatacggaag aaatccaaaa aaaatttgca
     8101 gaatacttga tgctggaagc taaaaaaaga agaataaaat caatttttgc ccagttttaa
     8161 tcaaaaacag taatagaagg agaaatacaa tgagttgttt aggaaatgtc atctggttta
     8221 tattcggcgg actaatcggt ggtcttagct ggttagcagc ggggatactt tggtgtatca
     8281 cgatcatagg tatcccaatc ggtatgcaat gttttaagat cgcctccctt tcttttgcgc
     8341 cttttggcaa agaagtaatt tatcaccagt ctggcatgtc attgatcgtt aatatcatct
     8401 ggctgatcgt ttcaggccta ccaattgctc tcgcacatgt cgtcagcggc atccttctat
     8461 gtatcacgat catagggata ccttttgcca agcaatcttt caaacttgct cgattggcgt
     8521 tgatgccatt cggtgcagaa attgtataaa aagagaataa taaaggttag aaaacgcata
     8581 gagtcaggtg ggatacatgt atttcacctg actctatgtg ttttcctgta taagttctcc
     8641 catgtgactt ttacgtcttt tatttatatg ggattactgt ttatggaaag ttgaaataat
     8701 tttcattaaa gaactctttc tatatttttg aaagcgtagt ggagcttcga atatctggga
     8761 aggacatttt gcagggattg aaaaaagata ttgtcctatc gaaaaattaa tgaatttaga
     8821 caaattgcca gcaacaggct tcaaacttag ttgctatcct gtcaagatca agaaggcaag
     8881 tgcgggttgg ataagagcag gggcaatgat cgaagagaaa aagaaagaat aagccaaaac
     8941 atctgtatgg aagaaatttt ccgatgcaga tgtttttcat tttttctttc ggataccttg
     9001 acatatgaat aaccattcat ttataattag ttatgaagag atgttcatat gttgaggcga
     9061 ggaggtcatt aaaatgaaaa gttacagatt agaaggttta gattgtgcga actgtgcaat
     9121 gaaaatcgaa aaaggagttc agaagatcaa tggagtcaag gaagcaacgg taaacttcac
     9181 ttctggaaaa ctaacgattg acgcagagga agatcatttg gctacgattg aacaagaaac
     9241 aaaaaaagta gtgaaggaat tagaaccaga tgtcaaagta acagaaatag ataaggaaaa
     9301 agtatctgaa catggaaatg aaaaagagag aaatacgctg ttccggattt tattttccct
     9361 tgcaggtatc gctttattgc tattatttga ttttaatgaa ccaattcgct tgatagggta
     9421 tttattgatt tatctgctga taggatatga tgtagtgaag aaggcagtga tgaatatcgt
     9481 caaaggaaaa atctttgatg agaattttct tatgtctgtt gctacgattg gcgctatgtt
     9541 gatcggtgaa tatccagaag ccgtagcagt gatgctattt tatcaaatcg gcgagtattt
     9601 ccaaggattg gcagtcagtc attcaagaaa atcgattcgc gaattgatgg cgatccgtcc
     9661 agaaacagct cacgtgcaga cgacggaagg attgatgacc gtgaaccctg aagacgtgca
     9721 gatcggtcag tttgtacttg tgaagcctgg tgaacgtgta ccgttagatg gaacgatcat
     9781 cgaaggagaa tcattagtcg atacgtcagc acttacagga gaatcagtcc ctaaatctgt
     9841 atatgttggt gaaacagtat taagcggatt tatcaataaa aataaaccat ttttagttca
     9901 agtagaaaaa tcttatgaaa attctactat cagcaaatta ttagaattag tagaaaatgc
     9961 cagcagtaaa aaagcaccag ctgaaaattt cattactaaa ttcgcgcgct attacacacc
    10021 agtcgttgta ggattggccg ttttattagc tgttttacct ccacttgttg tttctggagc
    10081 ggcgttttct gaatggatct atcgagcatt gacattttta gtgatctcat gtccatgtgc
    10141 attagtgatt tccgtgccac ttagtttctt tggcggtatc ggaggcgcaa gtaaaatcgg
    10201 tgtattagta aaaggttcaa attacttgga gttgcttgcg caaaccgaga cagtggtatt
    10261 cgataaaacc ggcacattaa cgaaaggtga cttttcgatc cagacgatcg atacaaaaat
    10321 cgatccaaag aaattcatgc aatatattgc gagtgcagaa cagttttcta cacatccgat
    10381 tgctcaatct gttttagaag gctacgcagg tccgctttta ccaacaacta atatccagga
    10441 atttgcaggt gaagggatct tagctgaagt tgatggcaaa caagtattag caggtaatca
    10501 caaattgatg gaaagatttg aaatctcttt tcctttaagt caagaaatcg gtacattgct
    10561 ttatctagcg atcgatcaaa gttacagtgg ttatcttgtt attgcagata ccttgaaaga
    10621 agatgccgta gacgcattgg ttcaattaaa acaggctggt gtgaagaaca cagtcatgtt
    10681 gacgggcgac tcgaaaaaaa ttgctgatca tatcgggaag caagtaggcg tggacaagat
    10741 atatagcgaa ttgcttcctg aagataaagt gcagcggtta gaggaaatct tacaaagaaa
    10801 caataaaaag accgcatttg tcggagatgg aatcaacgat gcaccggtgc tagcaagagc
    10861 agatgtagga atcgcaatgg gtggattggg aagcgatgct gcaattgaag cagcagatgt
    10921 agtcatcatg aatgatcaac catcgaagat tgcagaagca atccatttag caaagaaaac
    10981 attgaaaatc gtcaaacaaa acattgtctt tgctattggg ataaaaattt tggtacttgc
    11041 cttaggtgct tttggttttg catcaatggg agatgcagta tttgcagatg ttggggtgac
    11101 agtattagct gttttgaatg ccatgcgaag cttgcatgta aaaaaagagg acgaatgaac
    11161 gtcctctttt ttttatatcc ttttcttaat gaaaaatgta aagaaaatag ataggattat
    11221 aaaataacta atgtttttaa ttttgcagaa aattcaaaat agcttgaaat tcaataaggt
    11281 caatgaaata aaagaaaata acttattttt ttgatggaaa aaatatgaaa aaacgatgtg
    11341 tattaaataa aagaatacac acataaaata acactgtctt gtaagtttgt taaaaattga
    11401 atatgattac atctggataa caaattttta tcataaagga gagaacgaat gaacaacaaa
    11461 caattacgct ggtttactgt tgcgttaatt gcatttaaca tggtttgggg catggggaat
    11521 gtagttaata actatgctca gcaaggagta tcagttgtca cctcttggct attgattttg
    11581 gccatttatt ttattccata cgcattgatc gttggacaat taggatcagc cttcaaggat
    11641 agtaaaggag gcgtcagttc atgggtcgaa aatacgacat ctaataaacg actggcatac
    11701 tatgccgctt ggacttactg ggtcgtccat attccttatt tggcacaaaa gcctcaagca
    11761 attctgattg ctggtggttg ggcagtccaa ggaaatggaa atatcgtaaa tacgatgtca
    11821 gtacaaatgg tagcagttat ttcacttatt atatttcttg cctttttata tttatcaaca
    11881 aaaggacttt ctactttgaa agtgattggc ggactggccg gaacagcgat gttcgtcatg
    11941 tcgatcttat ttattctttt agctgtaacg gcaccgttca tcaatccaac gatggaattt
    12001 gccacaccac atatgaatca agtgaaaaca tatattccaa catttgattt cagttatttc
    12061 acaaccgttt cgatgctagt atttgcagtt ggaggggcgg aaaaaatatc cccttatgtc
    12121 aactctacaa gaaatcctgc aaaggaattt cctaaaggga tgatcttctt agcagcaatg
    12181 gtagggattt gtgctgtatt aggctcagtc gcaatgggta tgctgtttgc tagtgataat
    12241 attcctaaag acttgatggc aaatggggca tacagtgctt ttcaaatttt aggtaattac
    12301 tatggtatcg gtaactcatt gatgatcatt tacgcattga caaacatgat tggacaaatc
    12361 tccgcattag ccttttcaat tgatgcacca ctaaaaatct tgttagcaga tgcggatcca
    12421 gaatttgtac caaactggct acgtaaacgt aatcgtaaag gtacattggt gaatggttat
    12481 cttctgacag gtatactagt aggaatcatt attttgttgc cgatttttgg tatcaaagat
    12541 atgaatgaat tagtaaaatg gttgacgaac ttgaactcag ttgtcatgcc aatgcgttac
    12601 ctatgggtgt tcttggcata tatgatgttg aataaagctt ataagaaatt caattctgaa
    12661 tataaatttg ttaaaaatcc aaaaatcggt tttatctttg gtgcttggtg ctttgccttc
    12721 actgcgtttg catgtatcct aggaatggtg ccaaaagtag attatactgc tgatcctaaa
    12781 gcatggtggt tccaattatt tacgaatatc atcacaccaa tcgtcttgat cttattaggt
    12841 atgatcttgc cagcaattgc tcgtcgagac aaaaacaaag aaatcactga accagaaaca
    12901 gtggttcagc cttaaaatag aaatacttgt tacaagaggt tgtgacagaa gtggtcagct
    12961 tcaagaaata agaaggaatt tacgaaaatt gcttctcaaa tttttgtgaa gtttcggctt
    13021 gtttttcgaa gaagctactt ctggaacacc gtgaatcaga ggttgtgaaa aagctgtcct
    13081 gcatcaagta ataagaacag ccaaacaaaa atagcccctc atatttttcg tttggctggg
    13141 cttattaccg cagatgcaag ctttggaaca ccgtttaagg aagaggttgt aaaatcagcg
    13201 atttgacctg agtattagag aaacaaacgg aaaaatagct cctcatattt tgccgctttt
    13261 tgagctaatg ctgaaggtca gctgattgaa caccatttat tc
//
LOCUS       DANKEF010000068        12614 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000068 DANKEF010000000
VERSION     DANKEF010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12614
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.068"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            130..1260
                     /locus_tag="PWZ48_002305"
     CDS             130..1260
                     /locus_tag="PWZ48_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HDL2261944.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            1352..2107
                     /locus_tag="PWZ48_002306"
     CDS             1352..2107
                     /locus_tag="PWZ48_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HDL2261945.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSIPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFK
                     LPKAVGKLLGSIQTNLPESVLLDCGMDFLKDIC"
     gene            2165..4204
                     /locus_tag="PWZ48_002307"
     CDS             2165..4204
                     /locus_tag="PWZ48_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HDL2261946.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGATVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGFDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDFDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4313..5464)
                     /locus_tag="PWZ48_002308"
     CDS             complement(4313..5464)
                     /locus_tag="PWZ48_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2261947.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(5618..6148)
                     /locus_tag="PWZ48_002309"
     CDS             complement(5618..6148)
                     /locus_tag="PWZ48_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HDL2261948.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6247..6597)
                     /locus_tag="PWZ48_002310"
     CDS             complement(6247..6597)
                     /locus_tag="PWZ48_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HDL2261949.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(6754..8283)
                     /gene="ahpF"
                     /locus_tag="PWZ48_002311"
     CDS             complement(6754..8283)
                     /gene="ahpF"
                     /locus_tag="PWZ48_002311"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDL2261950.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(8293..8856)
                     /gene="ahpC"
                     /locus_tag="PWZ48_002312"
     CDS             complement(8293..8856)
                     /gene="ahpC"
                     /locus_tag="PWZ48_002312"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HDL2261951.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(9000..9662)
                     /locus_tag="PWZ48_002313"
     CDS             complement(9000..9662)
                     /locus_tag="PWZ48_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2261952.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            9846..11492
                     /locus_tag="PWZ48_002314"
     CDS             9846..11492
                     /locus_tag="PWZ48_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDL2261953.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            11483..12565
                     /gene="uxuA"
                     /locus_tag="PWZ48_002315"
     CDS             11483..12565
                     /gene="uxuA"
                     /locus_tag="PWZ48_002315"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2261954.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
BASE COUNT         4165 a         2335 c         2414 g         3700 t
ORIGIN      
        1 aaaaggtttc tgaccttttc tttatatttt tgtgactgac ttatttgatg agacaaactc
       61 gctcctcacc tgacaagtgg attgctgtgt gaggaggaga tgcggtgaaa aaagatcgat
      121 tggtggattt tgggtcctta tttgactttg tccatgatcg gtcttttaga agtatacagt
      181 gccagttctt atcgtttatt gcaagccgat gaaaatacaa aaagtctatt attgcgtcaa
      241 ctaattttca tatttttgag ttggagcgtg atcttcttag ctcgttcagt caaactacac
      301 tatttacttc accctaaaat agcaggatac ggtttagcct tatcgatttt ctttttagta
      361 ttagtaagaa tagggatatt cggtgtcact gtcaacggcg cacaacgttg gatctctctg
      421 tttggcattc aattccagcc ttctgaactg gcaaatcttt ttttgatttt ttatttaagc
      481 tggttttttc gtgacggaaa tagtagccca aaagatctaa aaaaaccatt cctgattaca
      541 gtaggtataa cttttctgat tttatttcag ccaaagattg ctggagcatt gatgatcctt
      601 tcgattgcgt gggtcatatt ttgggcagcg gcggttccat ttaaaaaagg gatctatcta
      661 atcgttactt tttctgcatt gctgattgga gcagcaggcg gggtattata tttaggaaat
      721 aaaggttggc ttccacagat gtttaatcat gcttatgaaa ggatcgcgac tttaagagat
      781 tcttttatcg atagtcatgg tgcgggctat caaatgaccc attcgtttta tgcactatat
      841 aatggtggca tatttgggcg gggattagga aatagtatca caaaaaaagg gtatcttcca
      901 gagacagaaa cagattttat tttttcgatt attacagaag agcttgggct aatcggtgca
      961 ttgtgtgtgc ttttcttatt gttctcatta tgcatgagaa tcttttgttt aagcagtcgc
     1021 tgtaaaaatc agcaagctgg attgtttctt ttaggattcg gaactttgct attcgttcag
     1081 acgataatga acgtgggaag tatcgcgggg ctgatgccaa tgactggtgt gccattgcca
     1141 tttgttagct acggaggaac aagttatctg atcctttctt taggaatcgg aatcacgttg
     1201 aatatttctt caaagataca ggccgaagag ttggcgctct atcgacctga aaaacaataa
     1261 agagaaactg agaaactctc agtttgaatt atcataaagt acaatctttt atttttcaag
     1321 cgagaggttc ttttagaaag aggaattcgc aatgaaagta tctcaaaaaa tcatattatc
     1381 tattttgtta ctgcttacat tagtagttgg ctatttttcg attgagttta tccatggatt
     1441 ttcgtctgca aaacagagtt caactgtgaa aaaggtagag ccgaaaagta tccctaccac
     1501 actaaatgtg gctttgattg gttcagatgc tcgttcgaaa gacgaaaacg gtcgctcgga
     1561 ttcacttctg gttgcacaat acaaccagaa aacacaacaa gcaaaactga tctctatcat
     1621 gagagattca tatgtcgata ttccaggtta cggaatgaat aagataaatg cagcttactc
     1681 ttatggggga gttgatttat taaatcaaac attaaaggaa aatttcaaat ttgaagctcc
     1741 ctattatgca agtatcacat ttcaagattt tatcgattgc gttaatgaac tgtttcctgg
     1801 tggagtcaag atcgatgcag aaaaatctct agatttagat ggcgtatata taaacaaagg
     1861 agagcaagtg atggatggta atactttact ccagtatgct cgatttcggg aagacaaaga
     1921 aggagacttc gggaggatca gaaggcagca gcaagtgatc aaagctatct ctcagcagct
     1981 gaaagacgta acttcaatat tcaagctacc caaagcggtt gggaaattac ttggtagtat
     2041 ccaaacgaac cttccagaaa gtgtgcttct ggattgtggt atggatttcc tcaaagacat
     2101 atgttagaat aaacaggtat aaatagtgaa ataaaggaat gacaagcaag agaaggagga
     2161 aaaaatgaaa agaagtgaca agcacggcaa aaatcgaaca ggcgcttata ttgccggcgc
     2221 agtgatctta atagcagctg cgggtggcgg ttatttttac taccagcact atcaagaaac
     2281 ccaagcagta gaagctggag aaaagacagt tgagcaattt gtccaagctt tgaacaaggg
     2341 agattataac aaagctgcag aaatgacatc aaaaaaggcg gcaaataaaa gtgcattatc
     2401 tgaaaaagag atcttagata aataccagaa tatatacggt gctgctgatg tcaaaggact
     2461 tcagatatcg aatctaaaag tagataaaaa agacgattct acttacagtt tttcatataa
     2521 agcaaagatg aatacctcat taggtgaatt gaaagatctt tcttataaag gaacattaga
     2581 cagaaatgat ggtcaaacca cgatcaactg gcaacccaac ttggtttttc cagaaatgga
     2641 aggaaatgac aaagtaagcc tgaccacaca agaagcagca agagggaata ttatagatcg
     2701 aaatggtgaa ccattagcaa caacaggcaa actaaaacaa ttaggagtcg ttccaagtaa
     2761 acttggagat ggaggcgaaa aaacagccaa tatcaaagcg attgcttcct ccttcgactt
     2821 aacagaagat gctatcaatc aggcgatttc acaaagctgg gtacaacccg attactttgt
     2881 cccattgaaa atcattgatg gagcaacgcc agaacttcca gctggagcta ccattcaaga
     2941 agtagacggc agatattatc ctttgggtga agcagctgct cagctgattg gttacgtggg
     3001 agatatcaca gcagaagata ttgataagaa tccagaatta agcagtaatg gaaaaatcgg
     3061 tcgctcaggt ttggagatgg cttttgataa ggatcttcgt gggacaacag gtgggaaatt
     3121 aagcattaca gatgcagacg gtgtagagaa aaaagttctg atcgaacatg aagttcaaaa
     3181 tggcaaagat atcaaattga caatcgatgc gaaggcacaa aaaacagctt ttgacagtct
     3241 aggaggaaaa gctggagcaa ctgttgcgac aacgccaaaa accggtgatc ttcttgcgct
     3301 tgctagctct ccaagctatg atccaaacaa aatgacaaac gggatctcac aagaagatta
     3361 caaagcttat gaagaaaatc ctgaacatcc attcatcagc cgatttgcga caggttatgc
     3421 tcctggatct acgtttaaaa taattacagc agcaatcggt ttcgacaacg gcactatcga
     3481 tccaaatgaa gtgttgacga tcaacgggct taaatggcaa aaagacagtt cttggggatc
     3541 ttttcaaaca acgcgtgtca gtagtgatgt atcacaagta gacttaaaaa ctgctttgat
     3601 ctattccgat aatatatatg cggcacaaga aacgttgaaa atgggtgaga aaaaatttcg
     3661 tacaggctta gataaattca tttttggtga agactttgat ttgccaatta gtatgaatcc
     3721 agcacaaatt tctaatgaag atagctttaa ctcagatatc ttgctagctg atactggata
     3781 tggacagggc gaacttctaa ttactcctat ccagcaagca gcaatgtatt ctgtttttac
     3841 caacaatggc acacttgtct atcctaaatt gattgcagat aaagagacaa aagataagaa
     3901 gaatgtcatc agcgaaacag cagtacaaac gatcgtgcca gatctgagag aagttgtgca
     3961 agatgtaaat ggtacagcac attctctttc tgctttaggg attccgttgg cagcgaaaac
     4021 tggtacagcg gaaatcaaag aaaaacagga tgtaaagggg caagagaaca gtttcttgtt
     4081 tgctttcaat cttgataacc aaggttatat gatggtcagc atgttggaaa ataaagaaga
     4141 tgatgattca gcaaccaaac gagcatcgga actattgcaa tacctcaacc aaaattatca
     4201 ataaaaaatt gctttttaag tacagtaaaa ggactgtggc gttatcgtca cagtcctttt
     4261 ttcaaataaa cggttttcac gatgtctctt tcacaacttc tttttgattt actcacactt
     4321 cataaacttt ttttgtaaaa tatttgagaa caagtggaga aataagtgta cttaataaaa
     4381 tcacgataac taaaggtgaa tagtactgtg gttcgatcaa ttgactttgc tgtccaattt
     4441 gtaaaatgat caaagccatt tctccacgcg aaatcatacc agcaccgacc atcaatgaac
     4501 tatgttgaga aaaaccggca atctgagcac caaaataacc tccaagcaat ttggtcaaaa
     4561 tcgctacaag cgtcaatata gaaataaaga gtagttgttc actaaagtca gaaaaatcaa
     4621 cttctaatcc tacactgaca aaaaatactg gtataaatac tgcatatcct aaagcctcta
     4681 cattgtggta tacttcttct ttaacctttg tctgactgac agctatccca gcaaaaaaag
     4741 caccaatgac agagctaagc ccaacaagat ccgctacgta agacattcct aaacaaataa
     4801 ctagcgatac gatgataata gaagaatttg cgtacacttt ttctgcaaat gaaagtagga
     4861 aaggcgcaat ccatttgact aataagaaaa taaaaacaaa ataaattagc tgttcaatca
     4921 aaacaagcgg taaagaagca ttgctagaag cttcccccgt tagaaaagat aggctgaaac
     4981 ttagaactaa aacgactaaa atgtcatcta ccacagaagc acccaaaatt gttgatcctt
     5041 cttttgtatt cactacgttt agttctttta atacttctac tgaaatactg acagaagttg
     5101 ccgcaagaat aatcccaaag aaaatcgctt catttcccga aacttggaat gctttgcctg
     5161 tcaaccagcc aaaaacaacc gggaagatga tcccaagcaa agcaacgaac ataccaggtc
     5221 ggaaatattt tttcaaaaga gataaatcac tttccagacc agctaaaaac attaataaga
     5281 tcacaccaat ctccgaaaag tcatggacta gaatatcagg tttgacccaa ttcaatccag
     5341 cttggcctag caaaacacca acaagtaatt gtccaatcac tgctgggata cctaatctcc
     5401 gcgagatatg tgagccaatc gttgttgaaa tcaatatcag gcataaaata ccaataaatt
     5461 ccattctcat cattcctttg tataaaatat agcccgaact tagcataaca tacattattg
     5521 aaaaaatagg aaaatatact cacaaaaaaa ccaacaatcc aaaagatttt tttggattgt
     5581 tggtttttat acggtctatt ttaataacta aaaatgatta acgtatagtc atttttctta
     5641 ttctgatgaa attgataatc tcctggatgc ccatgaaaat caaaataaag ccgaatattt
     5701 ggaatagaag catcaaactt ttgaatggat tgaacaataa gacgaatccg ccaatcaaca
     5761 acagaatact actataaaca ctccaacttt tagcttttgt attcatgctg aagaacaact
     5821 ggaacaagct gttgatgata acagctaatc caagaagaat cggcaggata gaaacgattg
     5881 caggtgcaaa agcatagacg atcagagcta agatcaggaa aaaaagacca ctgaacagac
     5941 cgatactcca agaacctgtt ttccttttaa aacggtaagc ctcaaaaaga ttcaacaacc
     6001 caagcaaaac aagataggcg gtgatcagat aaccgataaa atgaaaaaca gcagaagggt
     6061 ttagcacaat cgcaatccca gaaataatat aaacagcagc tcttaataag gcgtattttt
     6121 gaatattttg tttaatcgtc tcaaacatat aagaaaacct cctttgactt attttaacgt
     6181 gaactagtca ttttaccaaa ttaaatgtta caaaatgagc gaaggagaag aatatctctg
     6241 gaaaaatcaa cttctgagga tcttttccat ttttttgcct tttgctagct catcgatcat
     6301 tttatctaaa taacgaattt ctttcatcaa gttatcctcg atgtcttcta ctcgtacacc
     6361 acaaacaaca cctgtaatca aggaacgtga aggattgggg acaggggctt ccgcaaaaaa
     6421 ttgttcgaaa gtcacttgtt tttgtagttg atattctagt tctgtttggg aataacccgt
     6481 cagccaacaa atgatttcat ctacttcctc ttttatacga tcttttctct ccgcttttgc
     6541 tacatacata ggataaacgc ttgcaaaact tgtcgtaaaa atacgatttt gcgccataat
     6601 cagtcacctt tctttttttc aaattattta ctacttatag tatataagaa aaagagaaga
     6661 aaaagcgatt attcaaaaaa acaaaaagga ttgtgacaaa attcttgtca ccatcctttt
     6721 ttccgctctc tgacttttta caatcttcat agctcaagtg cgcatcagat aatcgaatgc
     6781 tcctaaagct gcagtagctc ctgaacccat tgaaacaatg atttgtttgt atggactatc
     6841 tgtacaatca ccagctgcga aaacgccggg gatattcgtt gcgccatttt tctcggtcac
     6901 tatttcattt cttggattca tttcgatcgt tccatctagc caatccgtat tagggagtaa
     6961 gccgactaga atgaatactc ctgaaacagc tagtgtatgc atctccatcg tacggcgatc
     7021 gatataggag agagattcta cctgattcgt accgctgatt tctttcgtcg ctgcatgttt
     7081 gataacagtg acattcgcaa gagagactag tttatcctgt aagatttgat ctgcttttaa
     7141 ttctggtaag aattctagaa catatacatg ttttgctaac ccagccaaat cgattgctgc
     7201 ttcaacacca gaattaccac caccgataac agcaatctct tttcctttaa ataaagggcc
     7261 gtcacagtgt gggcaataag caattccttt atttttaaac tctttttcgc ctggtacatt
     7321 gatagaacgc cagtgcgcac ctgtcgcgat gatggcggtt tttgctttaa gtgatatacc
     7381 gttagctaag ccaatattaa taaaatgttg ctctttattt aaggaaacgg cctgttgatt
     7441 catcataatg tcgatagggt aactacgcac atgactttct acttgctgca tcaattttgg
     7501 accttctgtg taagaggtac caatcatatt ttcaatccct agtgtttcaa cgacttgtcc
     7561 gccgaaagaa tcagcgacaa gtcctgtacg gattccttta cgtgctgcat agatagctgc
     7621 actactagct gctggtccac cgccaatcac taatacatca taaggttctt tttcaaacaa
     7681 ctcttcttca atcaaagggc cagtgatttt ttcaacaatt tgttcgatcg tcatgcgtcc
     7741 gctactaaat tcttctccat ttaaaaacac agttggcact gccatgattt ttttatcatc
     7801 gatttctttt tggaacatcc caccttcaat catcgtatga gtaatatttg gatttaatac
     7861 agctaatata ttcaatgcct gtacaacgtc ggggcaatta tgacaagtca agctaacata
     7921 actgtcaaaa tgcaatgttt gtgttatttt tttgatttta agactcaaag attcactgat
     7981 tttcggtgcc cgtccactaa tttgtaacat agccagtaaa aaagaggaaa attcatgacc
     8041 gagggggata ccagcaaaaa cgattccact ttctttgcca actggattga tagaaaaact
     8101 aggtgtgcgc atcgaatctc ttttttccca agagatttta ggagacattt ctgctatttc
     8161 cttcgtaaat tcagctagtt tttgactatt ttcactatta tccaaactaa cagaaaaaac
     8221 aataggagat tcaatcagtg ctaaatattg ttcgagattt tgtttagttt ctttgtctaa
     8281 catatccacc acctaaatct ttccaacaag atcaaaactt ggtttcaatg tttcgccact
     8341 ttctttccat tttgccggac aaacttcacc tgggtgggta cgtacatatt gagcagcgcg
     8401 aatcttatca atcagtgcac tagcgtcacg accgattcca tctgcattga tttccatagc
     8461 ttgaacgatt ccgtctggat caataataaa tgttcctcgc tgagctaatc cttgctcttc
     8521 atccaaaaca tcaaaaatac gagaaatctg atgagacgga tcaccgatca tcacgtattc
     8581 gattttaccg atagcatctg atgtatcatg ccatgctttg tgagtaaagt gggtatcggt
     8641 agagcaagaa aagacttcaa cacctaaaga tttcaatgta ctgtattgtt cttgtaagtc
     8701 ttctaattca gtaggacaaa caaaagtaaa atcagctgga tagaagcaaa gaatactcca
     8761 gttccccata agatcttttt cactgacttc taaaaattct ccttgatgat aagcatgtgc
     8821 attaaattct tcgatttttt tcccaatcaa tgacatagta aaacctccaa taataattat
     8881 ttatttataa ttattgtaat ttagttggag ttgcaaagtc aatggatatt ttgttattta
     8941 ataaagatta ctcaaaaacg aaggttaatt ataaaaatag aagaggtcgc aagaaaatgc
     9001 tactgctgga aatactcggg ataacgttga gtaacagttt cctgttcttt cagcatcatg
     9061 tgtaagtgca atcccgtttg aaatcgagct tcttctggtt ttttatcaac taaagccttg
     9121 agtaaatcat aatgttgttc catgatcgtt tcccatggaa gcccctgaat ggtcactcgt
     9181 aaccagcgga aacgttctaa atgggtatta tggtcatcta accagttcca aacttctttt
     9241 cttcctgcaa tttcgaaaca agcttcatga aataaattat ctgcacggaa aaaagcgcgt
     9301 tcgtcattta ttttgactgc tttttcttgt tcttctaaaa tcgtttctag cactattcgt
     9361 gtttgtgtgt ccaatttgga acaacattca atcatgattt gctcttccaa ctgttctcgg
     9421 acaaagcgag cattttctgc agaaacaaga ttaatttttg aaacatacgt accactttga
     9481 ggtatcgtat acacgagttc ttgctgacgt agttgaatca atgcttcgcg aatgggagtc
     9541 cggccgatag caagatgttc ctccagtgcc ttttcagata ttttccttcc aggttctaag
     9601 tcagcataga taatttgttt acgaatcgct tgataagctt gagattgcaa gctgcttttc
     9661 atctttattc ctccaaaatc atttctatga acgtagtata acagaagagg tctgaaaaac
     9721 aatctggtat gtcacattaa ctagtaaatt tgtataaata gtaaaatttt ggttgacata
     9781 gcgattttta ttgcataaaa ttcacttgaa tacaaaaact aatatgttag tttaggaggg
     9841 gaaaaatgat tgatttaaac aactggagga actttatccc tgaattatct caaaaagaag
     9901 tagaaatacc tcattttgac cgtgcatcat taagagaaaa tggacaaaaa aatcctgtct
     9961 ggatccattt cggtggcgga aatctttatc gaggatttca tgcagaaatc gctcaacgtt
    10021 tagcggatct aggcgaattg tccactggtg tcattgtttg tgaaacattc gatgaagaaa
    10081 taattgaaag ggcttaccaa gattttgatg atttgattct tgaagtggtc atgccaagca
    10141 gtggagagtt cgtcaaacgt atattagctg cgacagctga cagtctgtat tgtcatccag
    10201 attgttctga acactttgat aagatgattc gacactttga aaatcctagt ttacaattag
    10261 ttacttttgc tattactgaa aaaggttatc gattgaaaga tacaaataat aaatatatag
    10321 gaccgattgc agtcgatata aaaaatggtc caaagaagcc atcacataca atgagtattc
    10381 ttgctgcgct actttataaa cgttttttat ctggagcttg gccgattgca cttgtctcta
    10441 cagataattt ttcacaaaac ggtagtcaat tacgtacttc tttgctaacg atcgcaaaag
    10501 aatggcaaaa ggaaggttat gtacaaaata catttatcca atacttatcg aatgaagaaa
    10561 ttgttgcgtt tccatggacg atgattgatc gtatcacccc gaatccttcc ttgtcagtta
    10621 gcaaaatgtt ggcaaaagag ggcttttcaa atatgtcgat tttccaaacc gcaaaaggaa
    10681 ataatattgc tccatttgtc aatacagagg acatccatta tttaatcatg gaagatcatt
    10741 ttcccaatgg acgtccgaaa ttcgaaaaag ctggagtatt cttgactgat cgtgcaacgg
    10801 tagataaagc agatacgatg aaggtgacga cttgccttaa tcctttgcat acgtctttag
    10861 ctattatggg ttgtttactg gggtatagga gcatagctga ggagatgaaa gatccagatt
    10921 tatcgctgct agtttctgaa atcgggtaca atgaaggcct cccagttgtg gagtctccag
    10981 gtatcattga tccaaagatc tttctggaag aagtaattac tgtgcgcttt tctaatccaa
    11041 atattcctga tatgcctcag cgaatcgcca cggatacctc tcaaaaggtg gcgattcgtt
    11101 atggtgagac gattaaaaaa tatatggaaa tagaagttaa agacccgcag acacttgtat
    11161 tcataccatt gactctcgct gcttggcttc gataccttat tggcattgat gatcaaggaa
    11221 aaccattcat cccgagtcca gacccactat tgacagactt tcagcataag ctttccgata
    11281 ttcaactggg tacgcaagat acatcacata ttcatgagaa aatggctcct attttgtcaa
    11341 ataaagaaat ttttggtgta gatctttacc aagcagagct tggcagaaaa atagaagaaa
    11401 tgttcatcaa gatggtagca ggaccgaatg ccgtacgaaa aacattacag gattacttac
    11461 aagaacatgg aggaaattta tcatggaaat gacttttaga tggtacggat cacaggaaga
    11521 aaagattacc ttagatgaaa tcagacagat tcctgcggtc aaagggattg taggcacatt
    11581 gatggatata ccagtaggag aggtatggcc tagggagagg atcaaagaac taaaagaaga
    11641 aattgagtct cgtgggttaa cactaaaagt aattgaaagc gtcaatgtgc atgatgccat
    11701 taaaattgga ttaccttccc gtgagcagta tatcgaaaac tataagcaga caatccgaaa
    11761 cttggcagaa tatggaatag aagtcatctg ctataatttt atgcctgtat ttgactgggt
    11821 gaaatctgac cttgactacc gcttagaaga cggctcatcc acgttagctt ttatatctga
    11881 tgatattcca gatgatccaa gagaaatcgt cgcgcgcatc gaacattcca gcaatgaatt
    11941 tgaattgccg ggatgggaac ctgagcgctt agctcatatc aaatccttgc ttgaagccta
    12001 tgcatcagta gacgaagaaa aacttcgaga aaattttgcc tattttctcc aatcaatcat
    12061 cccaacatgt gaagaggtag gggtcaaaat ggctgtacat ccagatgatc cgccatactc
    12121 gatttttgga ttacctcgaa tcgtaaagaa tcgagaagat ttagattggt tgtgccgcat
    12181 agtagacagc ccttataacg gaatcactct ttgtacaggg agtattgcag aagatccaga
    12241 aaatgatgta tatgggattt tagcagaatt ttgtcaaaga gatcgaatcc catttgcaca
    12301 tgttcgaaat attaaatttt tagctaatcg agatttctat gaggcaccgc ataaatccga
    12361 atacggttca ttagacatgt accgcatact aaaaacaatg tatgacaacg gatttaacgg
    12421 atacatccgt cctgatcatg gaagaatgat ctggaatgag acaggaagac caggatacgg
    12481 cttatatgat cgagcattag gtgctgttta tctaaatgga ttatgggaag caatcgaaaa
    12541 aggaaacaaa agtaaagata attagtgaaa aaaactgtaa ttttattatg taaataagaa
    12601 ggatgttcaa attc
//
LOCUS       DANKEF010000069        12427 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000069 DANKEF010000000
VERSION     DANKEF010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12427
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.069"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..190)
                     /locus_tag="PWZ48_002316"
     CDS             complement(<1..190)
                     /locus_tag="PWZ48_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261955.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="PWZ48_002317"
     CDS             complement(200..1015)
                     /locus_tag="PWZ48_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2261956.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="PWZ48_002318"
     CDS             complement(1277..1720)
                     /locus_tag="PWZ48_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HDL2261957.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="PWZ48_002319"
     CDS             complement(1854..2192)
                     /locus_tag="PWZ48_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDL2261958.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="PWZ48_002320"
     CDS             complement(2180..2557)
                     /locus_tag="PWZ48_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HDL2261959.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="PWZ48_002321"
     CDS             2781..3974
                     /locus_tag="PWZ48_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2261960.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="PWZ48_002322"
     CDS             complement(4140..4562)
                     /locus_tag="PWZ48_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2261961.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4957..5151
                     /locus_tag="PWZ48_002323"
     CDS             4957..5151
                     /locus_tag="PWZ48_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261962.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5148..5594
                     /locus_tag="PWZ48_002324"
     CDS             5148..5594
                     /locus_tag="PWZ48_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261963.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5804..6976)
                     /locus_tag="PWZ48_002325"
     CDS             complement(5804..6976)
                     /locus_tag="PWZ48_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2261964.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7182..7997)
                     /locus_tag="PWZ48_002326"
     CDS             complement(7182..7997)
                     /locus_tag="PWZ48_002326"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HDL2261965.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8147..8593)
                     /locus_tag="PWZ48_002327"
     CDS             complement(8147..8593)
                     /locus_tag="PWZ48_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2261966.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8590..9594)
                     /locus_tag="PWZ48_002328"
     CDS             complement(8590..9594)
                     /locus_tag="PWZ48_002328"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2261967.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9634..9963)
                     /locus_tag="PWZ48_002329"
     CDS             complement(9634..9963)
                     /locus_tag="PWZ48_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HDL2261968.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10089..12416)
                     /locus_tag="PWZ48_002330"
     CDS             complement(10089..12416)
                     /locus_tag="PWZ48_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HDL2261969.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3508 a         2430 c         2273 g         4216 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cggccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tactacatac aagaaaaaaa acggatacaa
     4621 atatcatatt gtcacaccgt caatagtgca taaacctgtt attatttctc tttttttcct
     4681 aagtattcta ttgatatgaa tcatttaatc gtttattata taaatgtcaa taatctaata
     4741 ataaaataac gtacatattt tattattgca tacttaataa cagaactata gcaaaaaatc
     4801 ccgtgtatca acacgggatt agattaagtt tcctcaatca ctattcataa cacaattatt
     4861 ataacaagta agtaaacaat tgaaaaggac atttattgaa aacggttgtt ttttacttaa
     4921 ggaggaaatt tagcgaaaaa attagttgca ttgtttttgg tcgcaggaag tttgttgagt
     4981 gggtgtggtg ctactacgtc tactactcaa agcacaagtt cagatcaggc ttccagcagc
     5041 agtaccacga tctccgaaag tacggcagat tcatcgaaag aacaagaaca aattgaaaag
     5101 aaaaaagaag agaaacaaaa agcggaaaaa gctgcactag aaaaaaagtg acagaagcgg
     5161 aatctgcttt gtcacaagct gaagcaaatc ctacaaaaga aaactatgca actgcttcag
     5221 cagctatcca agcgctacct gaaaacaaac aagagttgac aagccgtctt gctgcaattg
     5281 attccacaat caaagcaaaa gaagcagaag aagaacggaa aaagcaagaa gaaattgcac
     5341 gacaacaagc agaacaagcg caacaagaag caatcgcaca gcaacaagca gaacaggcga
     5401 ggcaagaagc aatcgcacag caacaagcag aacaggcgcg acagcaatca gcagctgaac
     5461 aggcctctca gcaggcacaa gccgcagctc ctgagcaaaa taatgaacaa actgtctatg
     5521 tcacaccgac cggttcaaaa taccatacac ataaatgtgg aaatggtacg tatacccctg
     5581 ccactttaag ctaagctcaa gctcgtggtt taaccccttg ctccaaatgt tatggaaact
     5641 aattcaaaag ttatactata ataataaaat tcttctgaaa cataaaaaaa tagaagatgg
     5701 cgttccagat agtaaaccaa aaataacagt ttacacgtcc atttcgtcag cttctctctt
     5761 ttttcattta gtctgttccc tctgcttttt tggacatctt cattcattct ttctcaatca
     5821 aatagtacac ctctttttct tttcgcagtt tatatcttcc ttttttcaat ttcattgaaa
     5881 actgtttcga atgtttatgt tttacatgga ttttaagcag gagctcctca tgttgcaagc
     5941 gatagttcct gccttttaaa tcagaaaatc tcactagtcc gtattcatca gaagctttca
     6001 caaatggaac tttttctcta gtaatatagt gtttgccgtt gagcgttttt acagaaaaga
     6061 ccattccttc gattggtcgt ccgtttgtgt tctttacata aaaagataaa ggatagtttc
     6121 gcttagcgat ggccaaagaa tctaaataag cgatgagtag ccaaactaag ccgattgatc
     6181 caagtagtat accgataaac caaataaaaa atttccattt tttccattga tcaattcctt
     6241 ggatcgcttt ttttgcctct tttgcttgat aaggaatacg atgccccgtc acaagcagtc
     6301 gatgtgtatt gaccatataa ggagtacagg taagcaaagt cacgagatct ctgccttttt
     6361 ggatacccaa agaatctact tcatcaggta agaccacttg gattttttct acttgatacg
     6421 ccaatgtctt tccattgatc tggatgaaaa actggtctcc ttttttcaac ttaggcaaat
     6481 ctgtaaataa ttttgcttct gggagtccac gatgcccaga aagaacggca tgggtgtttt
     6541 tcccgccagt cggataagaa ctttcttcca ataatgacgt tcctttttgc aaaaatattt
     6601 ccgttgtctg atcgaatata ggtaagctga cattgatttt tgggatttcg atgactccaa
     6661 gcatatgttc cataaaaaag gcattactag gtaaatcttt taatgctttt gcatcaactg
     6721 cttggttgaa actgcctatc cctggtgata cgttttgctc tgccaactgt tggtttttct
     6781 ttgtcatttt ttcctgctgt ttttttattt cctctgaatt cttattcgat gcttgctttt
     6841 gataatggat gatcatttgc tgggcaagaa aatcattcag tgaatcttct acaaaaggat
     6901 aagaaaagat cccgatccca acgactaaaa tgattgccat gaagatgtcc agtattctta
     6961 ttctgctttt attcatcatg tccacctatt ttctaatatg tatatgaaga ggttgtgaaa
     7021 tcagcgcctt gacctgagta ttagcgaagc aaacggcaaa atagttcctc ctattttgcc
     7081 gtttgttgag cgaataccaa gagaccgacc acttgtcaca acctttctcc ccatttgaat
     7141 cactatcgtt tatttatcgt atgatttttg tttccaatag tttaagcttc tgtttcttga
     7201 tggcgttttt tgaaatagat cactgctcct ccaactaaag cagtacctac caagatgaat
     7261 acgatgatcc ccataccacc agttgaaggc aagattcctt taggttggtt atcgatcgtt
     7321 gtttcatcag aagttgttgg atcatcatct actgtaaatt cagtgtacgc agcttcattc
     7381 ttaggtaatg cgtagccttc tggtgctttt gtttcttgaa ggcagtatgc cccttgtttc
     7441 agcccttcga ttgaaaattt cccttctgaa tcagaaatca atttcacttc ttcccagtca
     7501 gaacctgtga cttcatctga ccaagcatat gggtattctc ctgaggcaag gtttgctatt
     7561 tttttcatta atttaacagt tggatctttt gctcttagaa tagaaaattc tgcatcggct
     7621 aatttttctt gcgtgttcac atcttgtttg atgaagtgga aatcaccgta gttttggaca
     7681 ttttctacag taatcgtgtt atccgtagtt gtattttccc gattgactac accgtttgga
     7741 ttcccaagat tggcttcatc tgcgccatgg atgataccgg ttgtttgttg caattttccg
     7801 ttttttacta taaaagtagt ttctttttgt ggattggtta attgataacc atctggtgct
     7861 tttgtttcaa ctaaagtata attgccgtct attaatccat tgacactgat ggatccttca
     7921 gtatcagttg tgaatgtcaa cgctgactca gcaggatcat agagagcgac accagtgttt
     7981 tcatcaaaac cggtcacgaa ttgattctct gaatttttga tctggaattg cgcccctgct
     8041 aacttatcta cccctgaaaa gttcccagct ttctggattt tgatattgct ttctttgatg
     8101 acatttttag catataggtg aatcgtgtct aaaggtttgt cagggtctag ggtaagctta
     8161 acacgatatt tggcgacgtt tcgacaccgg gctttggtgt ttctacaaag acgtatacag
     8221 catctttccc atcagattta cctggaagag tcaggttagc taatccttca ccagaagtcg
     8281 ttacacttgc gaagggtgta gcgtctgctg gaactgctgt gtctttactt ggatagtacg
     8341 tagcagtgat gtcatatacg ttgaattcaa cgccgttcaa cggttcgcta ttcggaaatg
     8401 tcaattccgt tccgttgttt atttgatttt ctggtgctga agtgaatttc aatttatgca
     8461 aattaaccgt aacattttct ggtgtaggtg atacggctgc agaaacctct gcagttccta
     8521 ggccgagaac gaaaggaagc agcatgatag cagttcctaa aaacgtccat atttttgttt
     8581 tgatgttttt cattttattt ccctcctatt gtttcctttt tgttgttttt tataaaacca
     8641 agctgtccct gttgaaagga ctagcaataa ccctaaaatt gagaaacctc caagcatctc
     8701 ttcgcttgtt tttggaaagc ttcctccatt tttttcattt gatggaccat ttgtctgcat
     8761 ctgaactggc gtttttccat tattcgtcgg atttgctttt ggttcgttga tgatttccaa
     8821 tggtatagga tgaagcggat cataactgcc tttttcaact tcaaagagaa tggttgattg
     8881 actcaatata tatcctttag gcggtttaat ctccactagt tcgtaacttc caaaagacaa
     8941 tccatggaca gaaaatgttc catctttact ggaagtaaaa atcgttgctc ttgaatcatc
     9001 tttggctacc caatgattta tcccgttttc ttgcgctaag tattggtcat tttttttgac
     9061 tagaaattga gcatctgcta gtttttgttt cgtttgccga tccactttga caaattgttt
     9121 gcctcctgtt tggatggcaa gatgtgtttc tactgtttga ttgtcagtcg tcaaacgtac
     9181 atcgttatca aatgtcgttt tttcactgtt ttctttcaat gtcatttggt aacggatcgt
     9241 taattttttt ccagcaaacg aactaagatc ttttccagaa tctgataaga caaaaccatg
     9301 ttttattttt tgtgtgtgaa atgtatgaat cttgtctcca ccaatcaaaa aatcgatcgt
     9361 ttctggttct agccataact gaggatcagc tgtatctttg atcgtatagg ttcctatctc
     9421 gttgatgttc tcaggaattg ttacggtgat gagataaggg actttatccc catctgcaaa
     9481 atctgtctgc ttattctcta ctgtttttgt taatgtgagt gcagcaggta ttttttgttc
     9541 gctctttgga tacaagtgga tggtcgtcaa tttatgatta gatgaatctg tcacaggaag
     9601 cacaacgaca aatggtgctg atttttcttt tacttaatct gaagtcttta tttccgtaaa
     9661 tcggtaaaca gcgtcacgac cttgctcatc tttgtccgat gcactaaaag aagcaatgcc
     9721 ctcttcctcg tctactgttt ttgtcacatt ttctgccagt attttttcac tatcactttt
     9781 ttgtgctagt tttctttgtg cgtcttccac actacttcct tctgaccgca gcttgtaaaa
     9841 ttcatccgag acatcatata tagcgaaagt aacgccgttc aaccctttta ctccttgaaa
     9901 gttatccggg atatttattc cgtcgttttg actgatactt ggcatcttat cgctttcgaa
     9961 cattcgatta tgcaaagtaa atgtaatagg tgattctcta gctgacacag tcggagaacc
    10021 tataaaaaag accagtagga taaaaaagag aacagcaaat atatccacac gtacattttt
    10081 tgtttttttc atcgctgttc acctgctttt ttcttacgat ggataaaata aaagaagcca
    10141 gaaaaaataa agatcagtcc taaaaagaca aagggcagta gacctatccc acctgtcgaa
    10201 ggaagcaaga gagtcggatt gatcgtcaac ttagcggtat ctgttgcgat aatcgtataa
    10261 ttattgttta cacttccttc gcctaaacta atcgttacta tttgtgatcc aatatctgtc
    10321 cggctgttaa ctgttagggt ataatccaat gattcgccta agacaacacc ggtaacgtgg
    10381 agatttccaa ccgaaatcga gtgactttgg gcatcataat taaaggcagc gtcatcgaca
    10441 gtaatcactg cgttagcttt atagatggta taagttcctg atgtgttggc tatatcagta
    10501 aatgacaact tgggatttgc tgctttgact ttgtctattc cgctattcgt tagtttaatt
    10561 gtatagctgc cagcattaac catatttttt gctgattgtg catctgctgt gtaatccata
    10621 ctgctaagtg ccacttgtac tggagtaagc gaactagtga caccctctgc tagcttgaca
    10681 tggattgacg ggacacctgc ttttccatca tatgtcttac tgccactgag ctctaccgca
    10741 ccgctgattt gcttaatcgt atagacatca gatgtctgca cgatagtaaa taaatagttt
    10801 gttctggcag ctagtttagc tagtccgctt tttgtcaatt gccaagacgt ataggttcca
    10861 atttcagtag gcgcagttac ttcaactccc ttttcattca tcgctttgaa atcagttgga
    10921 ttccatgtga tttgatcaat actgagatca ttgtttaaaa gggatgttga ttctgtttta
    10981 tcattaaagg catatttaac gatcggtaca acagaaagac catcatagta cttactgcca
    11041 tttgcattga ttgatactgt tgcatttgtc ttataatata ggatgacttc agaaatctgt
    11101 tttccatttg cgtcctttac gattacattt ccatttgtat ctaaactagc taaaacactc
    11161 attgctgatt ggttctttgt ttctatcgct gaaacagctg taaaggtata tccgttgata
    11221 gtaggcgtat tagtcgtcag gtcttgtgca gcacttccgt aagtaagatt cattatttta
    11281 gaaggagcaa tcatctgtcc aaaatagtct tgatacttaa tagtgattgg tgtttgtttg
    11341 cctgtatagg tcaccgtatc gacgttactt tctagggctg tgaaagaacc gtttgtttgt
    11401 gcaaaagaat agcccgttac tgctgttgga gtgataacca ctttgtcacc cacatggatt
    11461 ccagataatg tagtcccgta cgttccgatt tttgctgctc caccattgac tgtataagaa
    11521 aaagttcctg tccgattcgc attgacctga accgttgctg cttgataggt tggaaaattt
    11581 gaaacatttc ccttaccatc aacgatctgc ccatttttga tcgagtaccc cgtgaatgga
    11641 atatcatttg ctaactgtat accgattgaa aaagtactca tacctcctcc ggcaggaagc
    11701 cctccccatg ttgagggtgt aaaagaataa cttttatccg cattgggtgt aactgaatcg
    11761 atcgtatttc cagcggtagc cacccccata tagatacttt ttgggaatgt gatagtagta
    11821 ccatttactg tggagccaat aaacagatta tggatatctg gacggttatc aataaaagcg
    11881 attgctttgg ttacgccttg taacgcagtt ttatccgcac tcatcgatcc gttaaccgca
    11941 acaatgactc catctttggc atatatcact gtatatccat ctggtatggt atgtccgttg
    12001 aaattcgcag tggccgcaac agtactaaat gtctggcttc tacctgtttc tgaataagct
    12061 ttctcccctg ctgcttttgc tgctgtgatt tctttttctg tcggatcaat cagttgagtc
    12121 gaggatgtgg ttgaggttgt ctcgttgttg ctagactgat tggtgctttc tatggactgt
    12181 atttcagaat agggtggact atttgtctcc tctgtcgttt ggttagcaaa atttgacgtt
    12241 tctgtataaa cgtttacata aatacttgat aagctcccta agaaagtaaa aatcaagatg
    12301 atcgaaatca taatgggatg taagtatttt cttttagata cagctttgag tctagaaaca
    12361 aaatgttttt gacatatacg ttggatttta ttttgagttt tcttaatcat tttcatccct
    12421 cctctca
//
LOCUS       DANKEF010000070        12143 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000070 DANKEF010000000
VERSION     DANKEF010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.070"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..565
                     /locus_tag="PWZ48_002331"
     CDS             <1..565
                     /locus_tag="PWZ48_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HDL2261970.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            966..1571
                     /locus_tag="PWZ48_002332"
     CDS             966..1571
                     /locus_tag="PWZ48_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HDL2261971.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1587..2159
                     /locus_tag="PWZ48_002333"
     CDS             1587..2159
                     /locus_tag="PWZ48_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2261972.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2411..2668)
                     /locus_tag="PWZ48_002334"
     CDS             complement(2411..2668)
                     /locus_tag="PWZ48_002334"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261973.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2794..3609)
                     /locus_tag="PWZ48_002335"
     CDS             complement(2794..3609)
                     /locus_tag="PWZ48_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261974.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4126..5451
                     /locus_tag="PWZ48_002336"
     CDS             4126..5451
                     /locus_tag="PWZ48_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HDL2261975.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5444..5794
                     /locus_tag="PWZ48_002337"
     CDS             5444..5794
                     /locus_tag="PWZ48_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261976.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5791..5961
                     /locus_tag="PWZ48_002338"
     CDS             5791..5961
                     /locus_tag="PWZ48_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261977.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHKYEYYIGY"
     gene            complement(6865..7140)
                     /locus_tag="PWZ48_002339"
     CDS             complement(6865..7140)
                     /locus_tag="PWZ48_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HDL2261978.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7112..8065)
                     /locus_tag="PWZ48_002340"
     CDS             complement(7112..8065)
                     /locus_tag="PWZ48_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2261979.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8677..10170
                     /locus_tag="PWZ48_002341"
     CDS             8677..10170
                     /locus_tag="PWZ48_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2261980.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10305..10601
                     /locus_tag="PWZ48_002342"
     CDS             10305..10601
                     /locus_tag="PWZ48_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2261981.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10702..11553
                     /locus_tag="PWZ48_002343"
     CDS             10702..11553
                     /locus_tag="PWZ48_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261982.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11574..11857
                     /locus_tag="PWZ48_002344"
                     /pseudo
     CDS             11574..11857
                     /locus_tag="PWZ48_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4170 a         1864 c         2268 g         3841 t
ORIGIN      
        1 ttattttaag tctgaaaagg atacatcgtc tctaacgtta actttagagg aaggagttga
       61 acctgacaag gtgacctcta aagtgataaa ccaattgaaa gaaaaaggta gtttgaaaca
      121 tttgggggaa tatgaagtgt tagatacggc tatgttaacg aaaggaattg gtcaaatcct
      181 cagtacgatc acttatttta taacagctgt agcaggtata tctttattta tcgcaggggt
      241 aggtgtaatg aacatgatgt atatatctgt atctgaacga acaaaagaga tcggtataag
      301 aagagcccta ggagctactc gaaaatcgat tatgctgcag ttccttttag aaggtttgat
      361 tttaactctt tcaggagggt taattggcta tttattagga atggctcttg cttatagtgt
      421 tggttctttg ataaaggtaa acgtgtcact tgatctattt actattttac tagcggttgg
      481 ggtatcttcg gttattggac tggtcttttc tgtaatgccc gcttcagagg ctgcaaaaaa
      541 agatttaatt gatattctta gataaaatca gaatgacaag aatatttacg ttctagattc
      601 taaaataaaa cacaaagaga ccaaccaggc accctattgt ttctttgtag tatagagata
      661 ttctttggct tgtaagagat caatagagtt aggtgaccgg agttcgaaat aaaggaaatt
      721 tttgattgta taggagaatt ctttatccct aatatttaga attttgagta tgccatgttg
      781 atgttttaga ttctctataa atatagaggt gtatcaaagg tcaactagac tgtgcaaaat
      841 agagacactt gaaaaatcta tcgggggtca gaaacttaat agtttttgac acataaacaa
      901 aatcgcgata taatggggtt gagagtgttt cataaactta tgtcataaag ttaaggagat
      961 ttcgcatgat tttagagaat aaattgggat taaccaatca agttgagttg gctaaggttg
     1021 aagaaaaact cagtaaacaa aaagcgaaag aattgtacga ttgtggaaaa ataaatgaaa
     1081 tcgaagttgg tacatttaaa ggtttatctg aaattcatga atttctattt tcggatattt
     1141 acgattttgc cggaaaaatc cgatcggtca atattgcaaa aggaaatttt cgatttgcac
     1201 cagttatgta tttagaacat tcgttacagc atattgatca gatgccacaa acttcttttg
     1261 aagagattat caagaaatat gtggagatga atatcgctca cccatttcga gaaggaaatg
     1321 gccgaagtac tagaatttgg ctggatctca ttttaaagga agaattgcaa aaagttgtag
     1381 attggaactt aatcgataaa gcagactatc tttctgcaat ggagcgaagt cccataaacg
     1441 atttggaaat acgctatcta atttccaatg ctctgactga taaaattgac gatcgcgagt
     1501 tatatatgaa aggaatcgat gtcagctatt tctatgaagg gtattctgag tatacgatag
     1561 atgatttgta aaagtaggtg gaccaaatga cgaaatatgg atatgcacgt gtctcaaccg
     1621 cttctcaaag tacaaaagaa caaattcaac agttgcttga gaacggcgta gagaagaaga
     1681 atattttttc agaaaaattt acgggtacaa aacgggatcg gccaaaattc aatcagctgt
     1741 tggctggtct gaagcctgga gatgaaatag ttgttacgaa gcttgatcga tttgctcgta
     1801 acacgagaga agcattagag atcattgaac ctttactcga tgataacgta acgatcaagg
     1861 ttttgaatct aggaacgatt gaaaatacgt ccatggggcg aatggtgaca cggacgttgt
     1921 tgagtgtagc agaaatggaa cgtgatatga ttgtggaacg tacccaagaa ggaaaaatgt
     1981 ttgcaagaaa gaataatcct aatttcagag aaggacgtcc gaaagcaaca atcacaccga
     2041 agaagaggca cgcatatgaa ctgcttactt caggtaaatc gtataaggaa gttgaagcta
     2101 taaccggttt cagtcgcagt acgcttttta gaataaagaa gaaaattgaa gaaagttaaa
     2161 aaagaagtct cagcgaacca gacaggtaaa tacttaatgc gttctagata tgagtctatt
     2221 agcatgttgt gaaatataat atttttttat ttacttgatt tctgctcaaa aaatgtgtat
     2281 ggtaactgta aaaggtaaag taattaaagg tatgctagcc acgcttacta tattaggata
     2341 ccatcatata tttataatga acgatttgtt cgtctaccaa acaacacata tcgttcagcg
     2401 taagtcacgt ttaggaacgt ggctttttta gtgttattat cgataaataa tctattgact
     2461 ctattgattt tatctccata tccctacggt gttgattgtc tttagatctt tcatttaaga
     2521 accaaataac tatcataaaa atcagcactg ctgctaatat aataaacagc attcctattg
     2581 tgatattacc tattgatttt accataacta cgattaaagt aataatggca ttcaagtctc
     2641 ctttgttctc tatatataaa ttagccataa atcatcttcc tttcaaatta agatacttgt
     2701 ttgataccaa taaatagtaa gcgtggctag catacctttt tctgtaatag caagtgttct
     2761 aatagtccaa acttaatgat ggaccgatgc tagttatagt acagtaatgt tttctttctt
     2821 atctagattt ttagtaagct ctatcagcaa atctaacaaa tctgagtcct tttcagaaac
     2881 taaattaaat ctagtgttta caacaaagtt ttcatgttct atattgttta gattatttgc
     2941 ggaaaggagg gcttctagat cttgacattc aaatctatat gcatcgtcat aaatcttact
     3001 agaaggagtg cttaattcga aattacttat tttctcatct tggaccaaga taaatttata
     3061 gatagtatct ttttgtgttt ttcttttaaa aacacaacag agtgtgttat aaggaacgtt
     3121 ataattttca tcagcctcat tttttctcca ttcaaacaaa tgataaactg caaccaagtt
     3181 gccatcaatt tcatggacag tgctctcggt aacatccttt aaagaactat caagggctaa
     3241 tttatagtaa ctagagagag gaagtaaaat tgtcgattta ctgagatggt tatcgtctat
     3301 acgtgcaatc atacctaaat caactaaggt tttattccag gtgtttatag ttttagttgt
     3361 cactcctaga gactctgcta tggtattgac cgaatgccag gaatccccgc cttcattctt
     3421 tgccttaaaa cagtaataca gatataaatt aatagctttg ttattcggta aatatttttc
     3481 aagttctgtt ggtagaataa agtaagaatc cttaatgttt tttctgaact gtctccagtc
     3541 tctgtagttt tgtttataaa gttgtgaaac ggtttttcca cctttagaaa gtcctgctat
     3601 cttaaccaat cttattgctc ctttcttgaa tctgtcatga tcatacagta ttttttactt
     3661 gtagtccagc accttaatat attatcctat gtaattaatt tcgtactaga tagtaattat
     3721 tttttattta attctctagt cacttacaaa taagttatat aaactacgat taataaatta
     3781 ttacaaaaac tattaagaaa acttctacat acaactatta caaaactatt aagaaactga
     3841 atactcctat tttttgtttt taaggaaagt tttttctttt ttgaatttct tatttttttc
     3901 taatataact aagcaaaact ggataatacg acacttattt tcaaattttc acaagactaa
     3961 tagtaaatat ccatttaata aagtgataca atgtgttgat tttacaaatc aacagaagaa
     4021 gtagggaaat aacttaccta tgcttctttt tttgtacatt taatcgattc ttctttacaa
     4081 aaacgaacgt atgtttgtat aatgtggaag aatggagtgt tatttatgat ggattattca
     4141 aaagagccag taaatgatta ctttttgatc gatatgaaaa gcttttacgc aagtgtcgaa
     4201 tgtatagaaa ggaatttaga tccattaacg actgaattag tcgtcatgag tcgagcggat
     4261 aatacaggat cagggttgat tttagcttct tcaccagctg caaagaagaa gtacggtatt
     4321 tctaatgtca gtcgaccacg agatctaccg caaccttttc ctaaaacgtt acatgtcgtt
     4381 ccaccacgca tgaatctcta tattaagcga aatatgcagg taaacaatat tttcagaagg
     4441 tatgtagcag acgaagattt attgatttac tcgatcgatg aatcgatctt aaaagtgacc
     4501 agatcaatga atctttttac gactgaagga acaagaagtc aacggagaaa gaagctggct
     4561 caaatgatcc aggagaatat taaagaagag ctaggattgc ttgctacagt aggtgtcgga
     4621 gataatccct tattagctaa acttgcttta gacaatgaag caaaacataa tgatggattc
     4681 cttgctgaat ggacgtatca aaatgttcct gataaagtat ggaatattcc tgaaatgaca
     4741 gacctttggg gtattggatc tcgaatgaaa aaaagactca accaaattgg tatcatgagt
     4801 attaaagact tagctaattg gaatccatac actattaaga gccatttagg tgtgattggc
     4861 ttacagctgt attttcatgc gaacgggatc gatcgaacag atatagcgat cccaccagaa
     4921 ccgacaaagg aaaaatcata tggtaattca caagtgttac cacgagatta taccaggaga
     4981 aatgaaatcg aactggtggt aaaagaaatg gcggaacaag tagctattcg tatcaggcaa
     5041 cataactgta aaacaggttg tgtccattta tacattggca cgtcgatctt agaaacgaga
     5101 ccaggttttt cacaccagat gaagatccct ataacagata atacaaagga attgcagaat
     5161 tactgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca cgttggaata
     5221 acctatagca agcttgtgta cactgattcg cttcagctgg atttatttag tgatccacaa
     5281 aagcaaattg acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
     5341 tttacgtcga ttgtacatgc ttcttctatg ttagaaggcg ctcgtagtat tactagaagt
     5401 acattggttg gtggccatgc aggcggcgca ggagggctta aaaatgattg attataatga
     5461 tcgtggacgt ttaaagtggg caggttttta tttatctgat catactgaaa aaattgatgc
     5521 cgacaatgaa caacgagcca atcaaaattt ggcaaaagaa caaatgacga ctgaagaggt
     5581 tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatgtattc aaaaagaaga
     5641 acgaaacgct gaaggatact atccagaaga tattgttagc aagatcttag gtagtgatga
     5701 gctggggatc tacgttggaa aaaacaaagt tcactacgac gaaatacgtc atatcgaatt
     5761 ttaccaggat aataaatggg gtcgattaga atgaacatta tccagcaata tgagctaaag
     5821 tatataacct ttgatcagct atcagaagag atttggggtt atggccagcg tttgataaat
     5881 gaggttggtg ttgaacgctt ctctttctat gtagaagctg cagcaggata ccataaatac
     5941 gaatactata ttggctatta aagtgcaata aaattatatt tcttttatgt tttacatttt
     6001 gtatcaattc tgataaaatt acctataggt aattaaaaaa tataaaaaaa ctagttacaa
     6061 ccttaggacg ttgtaactag cttgaagcat actagagcta actaaaatag cactagaaag
     6121 caatgttaaa cccgtccaac aaacttgatt taacattgag accagcttat tcagctaaat
     6181 atagttgaat aagcttcttt tgttttaaaa aaacaaaagg tgtactacga ataacgcaag
     6241 taaaagaaat actattacaa ccagtaggct acaagtaagt attactaaac attgcgtagt
     6301 tagaaccttt gctctattgt ctaaagccac aaaattagag atcagcttta tgatgctaag
     6361 agcgagctgc atccatgcgg acataggttc ttttgacact attaattctc ctttttagga
     6421 taagggaaat tgctagtagt tttttcatta gcaccatccc ccttcatttg attgagtcta
     6481 gtgctgctgt gatagtttgc tgtttaatat gcttacattc ttattataac ataaatgaaa
     6541 caaacacatg gttagaggtc gtatcaatca aaatagtgtc tcacaaacga tttattactt
     6601 actcctgctg actaacggac tgttcttcag taactgtaac tatatgtttg ttaaaaccac
     6661 cattacctgt agataaaaac caatatattt actatactca ctatattaaa tatatttagt
     6721 atagtaaaaa ctagaaacaa taaaatttgt gaatgttgta tacccccaaa caatacacat
     6781 attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa atatatttac
     6841 tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata taatccgttc
     6901 ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt catacatagg
     6961 aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat ctacttgaac
     7021 tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa tatcatcttt
     7081 aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta ataatgccat
     7141 ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca gcatatttag
     7201 gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt ctttcttcta
     7261 attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa tcatttaatt
     7321 ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg cttttaaaaa
     7381 tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga gaggaacgtc
     7441 gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct tcaaaattcg
     7501 tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt gcgtaccgtt
     7561 ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga accacattat
     7621 ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat tcttctaaag
     7681 gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac aagtattcat
     7741 taagttcttc agatccagga gagccagcca taatatataa atgtggactt acttgagtaa
     7801 tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca aagctagtaa
     7861 tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa tcaattagca
     7921 acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg tctgtagtct
     7981 taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta tctgatgttt
     8041 taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa atatatttag
     8101 tatacttcat atacttaata tattcgatat atctaatata ctccaaattg ataagaattc
     8161 agactttata aggaatatag tggatatact aaatatattt gctatattca gtatatcaga
     8221 tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag agtgaaaaga
     8281 ggtaaactta attctataga aaccacagat aattcactat ttacaaagta gcaaaagcag
     8341 tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat tgattttagc
     8401 agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt ctcaagaata
     8461 tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc cgattgcctt
     8521 tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt gtacttaaat
     8581 taaatattct taaacactga agagagaaca catactatgg ctgagattaa agctaaactg
     8641 tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat ttattcagct
     8701 attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa acctgcaggt
     8761 catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca attaatgaat
     8821 gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga aaagaaacta
     8881 acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta tcgaacagtg
     8941 gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt tgatatcgat
     9001 tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt gggctacatg
     9061 ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact aaagacacca
     9121 ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa aatttcaaaa
     9181 acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg taaccacttc
     9241 ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa ctatcagtat
     9301 tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa aacaaaagaa
     9361 gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca agtagatgaa
     9421 ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg acgtcttggt
     9481 cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat
     9541 acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca
     9601 gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa
     9661 ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc
     9721 attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat
     9781 aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag
     9841 ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca
     9901 cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga
     9961 ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa
    10021 ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt tggactcaac
    10081 gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa
    10141 gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccttatacaa
    10201 tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata
    10261 cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt
    10321 atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag
    10381 tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa
    10441 aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga
    10501 ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa
    10561 tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta tttacttaaa
    10621 aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa
    10681 gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac ctggcttcct
    10741 ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc attaggaaaa
    10801 ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat tggagtattt
    10861 ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga catttttaca
    10921 gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa
    10981 tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga tttaattgtt
    11041 gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag aacaacaaaa
    11101 ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat gtatttgttt
    11161 gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat taaacgttca
    11221 attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga gtacaatgtt
    11281 gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag tatccatgaa
    11341 tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa agcaatagat
    11401 cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata tggcatcgga
    11461 aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt ttggaacagt
    11521 agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg aaaatgattc
    11581 ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag aaggagcagt
    11641 tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt acgatgaaca
    11701 ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg ttgattcgaa
    11761 agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg atgaatggga
    11821 acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg taaagagcat
    11881 aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt
    11941 ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg atagtgaagg
    12001 atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc tatccttttt
    12061 aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag
    12121 aaattgatcg aagatgtgac gaa
//
LOCUS       DANKEF010000071        11995 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000071 DANKEF010000000
VERSION     DANKEF010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.071"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(36..455)
                     /locus_tag="PWZ48_002345"
     CDS             complement(36..455)
                     /locus_tag="PWZ48_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HDL2261983.1"
                     /translation="MKKIIVATHYRLAEGFKSTLGYIAPNMVEIIAINAYLENTSVEN
                     QVQAALDQFEAEEQILVFTDLLGGSVNQTFAKFLDRYNIQLIAGVNLPILMTLALAIG
                     SNDLSETTIRQAIEESRQQIVYVNDLLQVSAMDDMDE"
     gene            complement(476..1306)
                     /locus_tag="PWZ48_002346"
     CDS             complement(476..1306)
                     /locus_tag="PWZ48_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2261984.1"
                     /translation="MNNERTKGLTQELNKKDRRNAAWRWSFIGSNNINYGTLQGTGYA
                     WSMAKTLRKIYPNDDDYVEAMRVEYEYFNITPYMGPLVAGADVALQEQQGTESLGAVR
                     SIKTSLMGPLSGIGDSLLWVLYPTIMGSISGYMALEGNPLGAIIWIILNLGLVWMRLK
                     LFDLGYESGIRLITDMADKLNAITESASVMGLTVVGSLIATVVKVYTPIEFTFGEVTM
                     GLQAEVFDRILPALLPVLLTALVYFLMEKKKWGFLKVIFLLLAFSLLGAYFGFLGVAP
                     "
     gene            complement(1299..2087)
                     /locus_tag="PWZ48_002347"
     CDS             complement(1299..2087)
                     /locus_tag="PWZ48_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2261985.1"
                     /translation="MPEFLQNILIILIPMLTLIDNRGITIINHWPVTVGLLAGLVMGD
                     LQSAMVIAGTFQLMSLGVAALGGSSVPDYGLATVVAIYLNARTGAEIGTSIAVGLPVG
                     ILAINLDIFSRTINSFVSEKSKQYLEERKFSKMQWINFISVFFVAMQAFIPMVILVAF
                     GQVAIETATQWIPEWITNGLNVAGGMLPVVGVAMLMRYMPTKRYIWALLAGFIMAAYM
                     ELPILAVSIVGLALAVFIYNNLTSQKVAVTTENAAYDEGDDFDE"
     gene            complement(2112..2585)
                     /locus_tag="PWZ48_002348"
     CDS             complement(2112..2585)
                     /locus_tag="PWZ48_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2261986.1"
                     /translation="MGLVLARIDERLVHGIVVTQWASAVKAKRIMVVDDQVAQDEPRK
                     AAMRLSKPAGTGMSLIDTETGITNFKAGKYDSHNVLLVVNNVKTLLDLSEQGIAIPKV
                     NIGIMLDRNDRKKLAKNFAASPEELQQLQQLADKGIPVSYQFAPSDKEEPLDKFI"
     gene            complement(2602..4659)
                     /locus_tag="PWZ48_002349"
     CDS             complement(2602..4659)
                     /locus_tag="PWZ48_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 31"
                     /protein_id="HDL2261987.1"
                     /translation="MLFKSKEVIVSEQSFVVKDRNQCLKVEGWGRDTVRVTCYPNHKG
                     FDPKQGIEELEKNVLTTATVTETSDQLILTNNRLTATYDGEKLVFYNNGTIILEEYSR
                     KQSQVRRTVGIDTHIPIPDEPTSSLNISPREFTYSGENTYQATLRFEGDPNEKLYGMG
                     GYQEENFNKNGGTYELMQRNSQTSIPFYLSDKQYGFIWNNAAIGEVTFGYNQKIWKSN
                     NTNLIDYLVTVGENPKQIIENYTQMTGRTPRLEPELLGLWQSKLRYQTLDELRTVYEG
                     YNQRGISLSVLVVDYYHWTSDGDFAFDSRYWEGIEDFAEEIKKTGTQLMVSLWPTVTE
                     ESRYYQSYRNNQLLIRSVTNKDSLFAGKALLDFFHPESKTFLRQVLDENYRSKGISLF
                     WADQAEPEMDVYQHKEYLIYEGSLEKYANKYPLHYLQAIQKNPERSNTEPTLIRSAWF
                     NAQKYGALAWSGDIESSFDSLRRQVQVGLSMGISGITWWTSDIGGFHSGNSQSPVFKE
                     LMIRWFQFAVFSPILRMHGDRQPHTQKIGNSGGGVRTSGGPNEIWSFGEEVEGILTRF
                     IHIRTRLKDYIRDVYNESATKGLPIMRSLFIEFPDDPKSWEETTHYLFGSDLLIAPVV
                     TEGATHIPVYLPKGCNWIQVFTKKVYPGGQIYKIAVTLNTIPVFCKEGAPLEHHLADI
                     FGN"
     gene            complement(4709..6766)
                     /locus_tag="PWZ48_002350"
     CDS             complement(4709..6766)
                     /locus_tag="PWZ48_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HDL2261988.1"
                     /translation="MITIEANRIVKKYDDELLIIEPWGQHSLRVRSFLDQHFVERENA
                     LTELHGYDEKLVETYYKNNQATIVNGKIKAVLDHRDRLTFYNEKDEVLLKEFIRLRAV
                     KHDDGSEDVGTVEFTKDFNSTLKLKSREYRANNDGSNFRTKVRFEPHAQEKIYGMGQY
                     QQNYLDLKNTMLELAQRNSQMSVPFYVSSRNYGFLWNHPGIGKVTFAKNMTEWEMYGT
                     DYIDYWITAGDSPKEITEQYTSVTGRVPEMPKKLLGLWQSKLRYRTPEEVLEVVEGYK
                     QRGIQLSTIAIDYFHWPKQGEYRFDPDFWPEPEKLVKTLKEDYNVEPLISIWPTVQSD
                     AYNYEQYLQGGYLIKVNRGVRLTMQIQGSTVFMDTTNPEARDYVWNLIKKNYKDIGID
                     YYWVDVAEPGYAVYDFENYRYHSGTAVESGNLYPNGYLKMVYDGNKDSNESVVTLVRG
                     AWAGAQKYGALAWSGDIDSSFEAFHNQVNSGLNMGMAGIPWWTTDIGGFHGGNPEDPE
                     FRELMVRWFQYSTFSPILRMHGDRLPHSKPLSNSGGGSMPTGAPNEIWSYGSEVENIM
                     TDYVEIRERMKDYLAESMTEAHELGYPIMRTLFYEFPEDKKSWEIDNTYMLGSDILVA
                     PIMNYKERGREVYLPSGEKWRHLFTGKEYAGGATYYVAAPLEQIPVFIKESASELFES
                     LGK"
     gene            complement(6789..8282)
                     /gene="xylB"
                     /locus_tag="PWZ48_002351"
     CDS             complement(6789..8282)
                     /gene="xylB"
                     /locus_tag="PWZ48_002351"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDL2261989.1"
                     /translation="MGYVLGLDLGTGALKGVLVDETGVIRHIEACDYPLHSPKGGYNE
                     QNPADWLTACGALFEKFSCNVSDFQAELAGISFSGQMHSLVVTDEAGTVLRPAILWND
                     VRNSAQCKRIMDEFGNEIIAITKNRALEGFTLPKILWIQEHEPAIWEKVRKIFLPKDY
                     LRYYLTGHSHMDYSDAAGTLLMDTTKKEWSKPILEKYNIPEVILPPLVESSACVGTLK
                     PEIKATFGFEKEVRIFTGGADNAVSSLGSGLTDSHTALASIGTSGVFLALEEEDHTRY
                     GGKVHLFNHVLPDTYYAMGVTLAAGHSVSWFKNLLAPEQSYDEFLRNIPNVPIGSNGL
                     LFTPYISGERTPHVDSQIRGSFIGLSNHHTRDDLIRAVLEGVTFSLKDSQQLMTSLKG
                     EEFKRIISVGGGAKNKTWLQMQADIFNTEVVSLDTEEGPGLGAAILAAVGLGWFDSFE
                     SCTNQVIRYGTTVQPIPENVEKYNTVYDLYTKVYGATKDLSHALSIL"
     gene            complement(8301..9608)
                     /gene="xylA"
                     /locus_tag="PWZ48_002352"
     CDS             complement(8301..9608)
                     /gene="xylA"
                     /locus_tag="PWZ48_002352"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDL2261990.1"
                     /translation="MNYFEAVPKIQYEGKQTKNKFAFRYYNPEEEILGKPMKEHLRFA
                     VAYWHTITADGSDPFGAGTYQRNWEGNNPMETAKNRVEAFFEILEKLGAEYFCFHDVD
                     IAPQGDSLEEFFQNIDTITDLIKEKMDQTGIKLLWNTANMFTHPRFVNGAATTNNADV
                     YAIAAAQVKKGLDISKKLNGQNYVFWGGREGYESLLNTNMDFELDNLARFYKMAIAYT
                     KKIGHQPQFLIEPKPKEPSKHQYDFDAATSMAFIMKYGLQDHFKLNLEANHATLAGHT
                     FEHEINVARSYNALGSLDANQGDLLLGWDTDEFPTDIYSTTLAMYEVLENGGIAPGGI
                     NFDAKVRRTSFEMEDLILAHIVGMDTYARGLRAAAKMKEERFFEEIMEEKYASFTQGI
                     GKKIIDDQETFESLSEYALSLSDVTNQSSHIEYIKSLINDYLV"
     gene            10040..11011
                     /locus_tag="PWZ48_002353"
     CDS             10040..11011
                     /locus_tag="PWZ48_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2261991.1"
                     /translation="MASVSGGRKPIYLSFNNECALVIAIEIGKDYIHSSLSYINGELV
                     NKLSYHSLLVTKDTLVMLLTKIISDLTSKIPQTPYGILGIGMAIHGIVKDDTVLFTPY
                     SDIDSIPLKDLLAKEIPYPIVLINEANAGALGEYTFKTPSENLVNINIQDGIGAGIVE
                     NGKLFIGANGTAGEIGHTTLYPDGILCPCGNHGCLEHYASTRILLKKLLDSDHKPHPD
                     ISVIVKKWHQGDAHTQKILKHNAKLLSIGINNIVLMYDPEVVIINSEIYRGIPELIDE
                     IKKHLTGKNTKNLAIKNTSLKGNSTLYGCVSIVAQSFLKINNLKFTV"
     gene            complement(11102..11413)
                     /locus_tag="PWZ48_002354"
     CDS             complement(11102..11413)
                     /locus_tag="PWZ48_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2261992.1"
                     /translation="MRNKPITVTLFANAYDNELKTNPHSKLKEGTYSVDNDRISFLTK
                     KGEGLVLNLNIQHIDSIATFNQSVIQIKDHNMTEVKNKIYAFSKKSNDKSYWETLAEI
                     I"
     gene            complement(11866..>11995)
                     /locus_tag="PWZ48_002355"
     CDS             complement(11866..>11995)
                     /locus_tag="PWZ48_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2261993.1"
                     /translation="NFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         3285 a         2653 c         2286 g         3771 t
ORIGIN      
        1 caaaaagatt gaacgaattc aatctttttg cttttttatt catccatatc atccatagct
       61 gatacttgca acaaatcgtt aacataaaca atttgttgtc gggactcttc gatggcttgg
      121 cgaatggttg tttcactcaa atcgttgcta ccgattgcta aggctaaagt cattaagatt
      181 ggcaaattaa cgcccgcaat caattggata ttgtaacggt ctaaaaattt ggcaaaagtc
      241 tggttaacgg aacctcccaa caaatccgtg aaaactaata tttgttcttc ggcttcgaat
      301 tggtctaatg ccgcttggac ttggttttca acagaagtgt tttctaggta cgcattaata
      361 gcaatgattt ccaccatatt tggtgcaata tagcctaacg tactcttgaa cccttccgct
      421 aagcggtagt gtgtagctac aataattttt ttcacctatt tccctcctcg tatgattaag
      481 gtgcgactcc taagaaaccg aagtaggccc caagtaaaga gaacgcgagc agcaagaaga
      541 taacctttaa aaagccccat tttttctttt ccatcaagaa gtaaaccaat gcggtcaaca
      601 agaccggtaa caatgctggt aagatacggt caaagacttc cgcctgcaag cccatggtta
      661 cttcaccgaa agtaaattct attggcgtgt aaactttaac aacagtcgca attaagcttc
      721 ccactaccgt cagtcccatg acggaagctg attctgtgat ggcgttgagt ttatcggcca
      781 tgtcagtgat taaacgtatc cccgattcgt accccaaatc aaacaatttc agacgcatcc
      841 agactaatcc gaggttcaat ataatccaaa tgatagcgcc taatgggtta ccttccagag
      901 ccatgtagcc actgatagat cccatgatag ttgggtacaa tacccatagt aaggaatctc
      961 cgataccgga caaaggaccc atcagggatg ttttgattga acggacagct cccaaggatt
     1021 ctgtcccttg ttgctcttgt agcgctacgt cagcaccagc gactaagggt cccatataag
     1081 gggtgatgtt gaagtactca tattcaaccc tcattgcttc cacgtaatca tcgtcatttg
     1141 gataaatttt acgcaaggtt ttcgccatgg accaggcata gccggttcct tgcaaggttc
     1201 cgtagttaat gttgttactg ccgataaaag accagcgcca agctgcatta cgacgatctt
     1261 ttttgtttag ttcctgtgta agtccttttg ttctctcatt attcatcgaa gtcatctccc
     1321 tcatcgtatg cagcattttc agttgttaca gccacttttt ggctcgttaa gttattatag
     1381 ataaaaactg ccagtgctaa tccaacaatt gaaacggcca agattggtaa ttccatatat
     1441 gctgccatta taaaaccggc taacaaagcc caaatgtagc gttttgttgg catatagcgc
     1501 attaacatgg ctaccccgac gactggcagc atacccccag cgacgtttaa accatttgta
     1561 atccattctg gaatccattg ggtggctgtt tcaattgcta cttgaccaaa ggctacgagg
     1621 ataaccatcg gaatgaaagc ttgcatcgcg acgaagaaga cactgatgaa gttaatccat
     1681 tgcatcttac taaatttccg ttcctccaaa tattgtttag atttttcgga aacgaaagag
     1741 ttgattgtcc gcgaaaagat gtccaagtta atagccaaaa taccgactgg taaaccgacc
     1801 gcgatggaag ttcctatttc tgctcctgta cgagcattca agtaaatcgc aacaacggtt
     1861 gccaaaccat aatccggtac cgatgatcca cccaatgcgg ctactccaag gctcattaat
     1921 tgaaacgttc cagcgataac catggcggat tgcaaatctc ccatcactaa accagctaaa
     1981 agaccaaccg ttactggcca gtggttaata atggtaattc cccggttatc aataagagta
     2041 agcataggga ttaaaataat taagatattc tgtaaaaatt ctggcattat aattcctcca
     2101 atctcaattt attaaataaa tttgtctaac ggttcttctt tgtcggatgg agcaaattgg
     2161 tatgataccg gaatgccctt atctgcaagc tgttgcaact gttgcaattc ttctggggaa
     2221 gccgcgaaat tcttagctaa ctttttgcgg tcgtttctat ccagcataat cccgatgttc
     2281 actttcggaa tggcgatgcc ttgttcactc aagtcgagta acgttttaac attattaact
     2341 accaacaaca cgttatgact gtcatatttc ccggccttaa aattggtaat gccagtttct
     2401 gtgtcaatca aggacattcc tgtccctgct ggtttgctta gacgcatagc tgcctttctt
     2461 ggttcatctt gggcaacttg atcgtccact accatgatac gttttgcttt taccgcactg
     2521 gcccattgtg tcacaacaat tccgtgaacc agtcgttcat caattcgagc taatactaat
     2581 cccatttttc atttctcctt ttcaatttcc aaatatatct gctagatggt gttcgagtgg
     2641 tgctccctct ttacaaaata ccggaatggt attcagtgtt acagcaattt tgtatatctg
     2701 accaccaggg taaacttttt tcgtgaaaac ttgaatccaa ttacaaccct ttggcaagta
     2761 gactggtatg tgtgttgcgc cttctgtcac aaccggagca atcaacaaat cgctgccgaa
     2821 taaataatga gtggtttctt cccaagattt gggatcatct ggaaattcta tgaacagact
     2881 acgcataatt ggtaaacctt ttgtagcgct ctcattatat acatccctaa tgtaatcctt
     2941 gagacgcgtt ctgatatgga taaagcgcgt caatatgcct tcaacttctt ccccgaaaga
     3001 ccatatttca tttggtccac cgcttgtcct taccccccct cccgaattcc ctattttttg
     3061 ggtatgaggt tggcgatcac cgtgcatccg tagaatcgga ctgaaaacag cgaactgaaa
     3121 ccagcgaatc atgagttctt tgaaaactgg ggactgactg tttccactat ggaaacctcc
     3181 gatatcgctc gtccaccaag taatgccact tatgcccata ctaagaccga cttgaacttg
     3241 tcttcttagt gaatcgaatg aactttcgat atcccccgac caagctaatg ccccgtattt
     3301 ctgggcgtta aaccaggcag aacggattaa ggtaggttct gtgttcgatc tctctggatt
     3361 tttctggatt gcctgtagat aatggagagg gtatttattg gcatattttt ctaagctacc
     3421 ctcgtatata agatattcct tgtgttggta cacatccatt tctggttccg cttggtcagc
     3481 ccaaaacaag gaaattccct tgctccggta attttcgtcc aagacctgac ggaggaatgt
     3541 cttactctct ggatggaaga aatctaacag agcttttccg gcaaatagcg aatctttatt
     3601 tgtgaccgac ctgatcaaga gttgattatt ccggtagctc tgataataac gactctcctc
     3661 ggttaccgtt ggccatagag ataccatcag ttgggtgcca gtcttcttga tttcttccgc
     3721 aaagtcctcg attccttccc aatagcgaga atcaaatgcg aaatcaccat ctgaagtcca
     3781 atgatagtaa tcaacaacca aaacggaaag agaaatgcct cgttgattgt aaccttcata
     3841 aaccgttctt aattcatcca acgtctgata acgcagcttg ctttgccata accccagtaa
     3901 ttccggttcc aaccgaggcg tgcgcccagt catttgcgta taattttcta tgatttgttt
     3961 cggattttct ccgacggtca ccaagtaatc aataagattc gtattgttgc tcttccatat
     4021 tttctggttg tagccaaatg ttacttcccc gattgccgca ttattccaga tgaaaccata
     4081 ctgcttatcg gagagataga atggtattga cgtctgggag ttccgttgca ttaactcgta
     4141 cgttcccccg tttttgttaa agttttcttc ttgatatccg cccatcccgt acaatttttc
     4201 attcggatca ccttcaaaac gaagagttgc ttggtaggtg ttctcgccag aataggtgaa
     4261 ttctcgaggt gaaatattta acgatgaggt cggttcgtcc ggaatcggaa tgtgggtgtc
     4321 aatgcctaca gtacgtcgga cttgactttg cttccgagag tattcctcca ggattattgt
     4381 cccgttgttg taaaagacca acttttctcc atcgtatgtg gctgttaaac ggttgttggt
     4441 caagattaat tggtccgacg tctctgtaac ggttgctgta gtcaagacgt tcttctccaa
     4501 ttcttctatc ccttgttttg ggtcgaaccc tttgtggttg ggataacagg taacgcgcac
     4561 cgtgtcgcgt ccccatcctt ccactttcag gcattgatta cggtccttca cgacgaagga
     4621 ctgttcggaa acaatcactt cttttgattt aaagagcata gcttctttcc tcccaattga
     4681 cttgtccttt atctcctctc tttatgggct atttgcctaa cgattcaaaa agttcagagg
     4741 cggattcttt aataaatacc gggatttgtt caagaggtgc tgcgacataa taagtcgctc
     4801 ccccagcata ttctttaccg gtgaataagt gtcgccattt ttcaccgctt ggtaagtaaa
     4861 cctccctacc tctttctttg tagttcataa tcggagcaac caggatgtca gaaccaagca
     4921 tgtaggtatt atcgatttcc caggattttt tgtcctctgg gaattcgtag aataatgtcc
     4981 gcatgattgg gtatccgagt tcatgtgctt ctgtcattga ttctgccaag tagtctttca
     5041 tccgttctct aatttccacg tagtccgtca tgatgttttc aacttcactg ccgtaagacc
     5101 aaatttcgtt tggtgcacct gttggcatcg aaccaccacc agaattggag agcggtttac
     5161 tatgtggcag acggtctcca tgcatgcgca aaataggaga aaacgtgctg tactggaacc
     5221 agcgaaccat caattcgcgg aattccggat cttccggatt accaccatgg aagcccccga
     5281 tgtcagtcgt ccaccatgga ataccggcca tccccatgtt caatccggag ttgacttgat
     5341 tatgaaaggc ttcgaaactg gaatcaatat ccccagacca agccaaagca ccatacttct
     5401 gagcacctgc ccaagcacct cgtaccaatg tgacaacgga ttcatttgaa tccttattgc
     5461 catcgtaaac catcttcaag tatccgtttg gatacaagtt gccactttcc accgccgttc
     5521 cggaatggta gcgataattt tcgaaatcat aaacagcata acctggttca gccacatcca
     5581 cccagtagta atcaataccg atgtctttat aatttttctt gattaggttc cagacataat
     5641 cacgggcttc aggattagtc gtatccatga aaacagtgct tccttggatt tgcatcgtca
     5701 agcgaactcc gcgattcact ttaatcaaat agccaccttg gagatattgc tcatagttat
     5761 aggcatctga ttggacagtt ggccaaatcg aaatcaatgg ttcgacattg taatcttcct
     5821 tgagtgtttt gaccagtttt tctggttctg gccaaaaatc gggatcaaat cgatactccc
     5881 cttgcttcgg ccagtggaaa tagtcaatag caatagtcga tagctgaatg cctcgctgtt
     5941 tgtatccttc taccacttct aagacttctt caggagtgcg atagcgtagt ttactttgcc
     6001 ataatcccag cagttttttc ggcatttcag gaacacgtcc cgtaacgctc gtgtattgct
     6061 ccgtaatttc tttaggtgaa tctccggctg taatccaata atcgatataa tccgttccgt
     6121 acatttccca ttcggtcatg ttctttgcaa aggttacttt tcctatacca ggatgattcc
     6181 acaagaaacc gtagttccgg cttgaaacgt aaaagggaac cgacatttgt gaattacgtt
     6241 gagccaattc gagcattgtg ttctttaaat caagataatt ttgttggtat tgccccatcc
     6301 catatatttt ttcctgggca tgcggttcaa atcggacttt tgttcgaaag ttcgacccgt
     6361 cgttatttgc acggtattca cgagacttca actttaaagt ggaattaaaa tctttggtaa
     6421 attcgactgt cccaacatcc tcactgccat cgtcatgctt aacggccctc agacgaatga
     6481 attcttttaa gagaacttca tccttttcat tgtaaaacgt caaacgatct cgatgatcaa
     6541 ggacagcttt tattttaccg tttacaatcg tagcttgatt gtttttgtag tatgtctcaa
     6601 ccagcttttc atcataccca tgcagttcag ttaatgcatt ttcccgctca acaaagtgtt
     6661 gatccaaaaa cgaccggact cgaagtgaat gctggcccca tggttcaata attagtaatt
     6721 catcatcgta tttttttacg attcgatttg cttcaattgt aatcatgatt tcctcctatt
     6781 agaattactt acaatatact aagcgcatga gacaaatctt ttgtcgcacc gtacaccttc
     6841 gtatataagt cataaacagt attatatttt tctacgtttt ccggaattgg ttggacagta
     6901 gtaccatacc gaatgacctg gttggtacaa gattcgaaag aatcgaacca acctagaccg
     6961 actgcagcca agattgctgc tcccaaaccc ggaccttctt ccgtatccag gctcacgact
     7021 tccgtgttaa aaatatcagc ctgcatctgt aaccaagtct tgtttttagc gccgccaccc
     7081 acactaatga tgcgcttgaa ctcttcgcct tttaaagaag tcataagttg ttgagaatcc
     7141 ttcaaggaaa atgttacccc ttccaaaaca gcccgaatca ggtcatcccg ggtatggtga
     7201 ttactcaatc cgatgaagga cccacggatt tgactgtcaa cgtgtggtgt gcgttcccca
     7261 gaaatatacg gggtaaatag tagtccatta ctgcctatcg gcacattagg aatgtttctt
     7321 aagaattcat cgtatgattg ttccggtgcc agtaaatttt tgaaccatga gacgctatgt
     7381 ccagcagcca atgtgacgcc catcgcgtag taggtatctg gcaatacatg gttgaataga
     7441 tggacttttc caccataacg cgtgtgatcc tcttcttcca gggccaagaa cacaccggaa
     7501 gtaccgatac ttgctaaagc ggtatgggaa tccgtcaatc cagatcccaa tgaggacaca
     7561 gcattgtcgg ctccaccggt aaaaattcgt acctcttttt caaagccaaa ggtcgctttg
     7621 atttctggtt tcaatgttcc aacacaagcg gatgattcca ctaatggggg taagataact
     7681 tcgggaatgt tatatttttc cagtataggt ttggaccact ctttcttagt cgtatccatc
     7741 aataaagtac cggcggcatc ggaataatcc atgtggctgt ggcccgtcag gtaatagcgt
     7801 aagtaatcct tgggtaaaaa tattttacgt accttttccc agatagcggg ctcatgttcc
     7861 tgtatccaaa ggatttttgg taaggtgaat ccttccagag ccctgttctt agtgattgca
     7921 atgatttcat tcccaaattc atccataatg cgcttacatt gggccgaatt tcgaacatca
     7981 ttccatagga tagccggtcg taagacagtt cccgcttcat ctgtaacgac caaggaatgc
     8041 atttgtccgg aaaaactaat tcctgctagt tcagcctgga aatcagagac attgcaagaa
     8101 aatttttcga acaatgcacc acaggctgtg agccaatcag cgggattttg ttcattatag
     8161 cccccttttg ggctatgaag ggggtaatca caagcttcaa tgtgccgaat taccccggtt
     8221 tcatccacca acactccttt aagggcccct gttcctaagt ctagccctaa tacataaccc
     8281 atgttttaca ccccgacttt ctatactaaa taatcattaa tcagtgattt gatgtattcg
     8341 atatgacttg attggtttgt cacatctgaa agggaaagtg cgtactcact aagcgattca
     8401 aaggtttctt ggtcatcaat aattttttta ccgattcctt gggtgaagct tgcatatttt
     8461 tcttccataa tttcttcgaa gaaacgttct tctttcatct tagcagcggc acgcaaacca
     8521 cgtgcataag tatccatccc tacgatatgt gctaaaatca aatcttccat ttcgaaggaa
     8581 gtccgacgta ctttagcatc gaagttgata ccacctggtg caatcccccc attttccaag
     8641 acctcataca ttgccaaagt cgtggaataa atatctgtag ggaattcgtc tgtatcccac
     8701 cccaacaaca aatcaccttg gttagcatcc aacgaaccca atgcgttgta actgcgggct
     8761 acattgattt cgtgttcgaa cgtatgtcct gccaacgtag catggttggc ttcaaggtta
     8821 agtttaaagt ggtcttgtaa accatatttc atgatgaacg ccattgaagt agctgcatca
     8881 aaatcatatt ggtgttttga tggttctttt ggttttggtt caatcagaaa ttggggttga
     8941 tggccaattt ttttcgtata ggcaatcgcc attttgtaga aacgagccaa gttatccaat
     9001 tcaaaatcca tgttggtatt caataaactt tcatagcctt ctcttccacc ccaaaaaaca
     9061 taattttgac catttaattt tttgctaata tctaaacctt tttttacttg tgctgcagca
     9121 atagcgtaga catcagcatt attagttgtt gctgctccgt tgacaaaacg aggatgtgta
     9181 aacatattag cagtgttcca aagtaattta atgcccgttt gatccatttt ttctttaatc
     9241 agatcggtaa tggtatcgat gttttggaaa aactcttcca aggaatcacc ttgaggagca
     9301 atatcgacat cgtggaaaca gaaatattcc gctcccaatt tttctaaaat ttcaaagaaa
     9361 gcctccacac ggtttttagc cgtttccatt gggttgttgc cttcccaatt tctttggtaa
     9421 gtacctgcac cgaatggatc ggagccgtct gccgtaattg tgtgccagta tgctaccgca
     9481 aaacggagat gctctttcat cggcttgcct agtatttctt cttcgggatt atagtagcga
     9541 aaagcaaatt tatttttagt ctgtttgccc tcgtattgta tttttggtac cgcttcaaaa
     9601 taattcattg ctaattccac ctttcttagt tagttcgaaa ctcgtactaa cctgatatga
     9661 ttatattacc tgggtgggaa cagcttgtca agcgctttcg ttttttaaaa attaccttgg
     9721 ttggttaggt gcgatttata ttttttccct gggacttcct ttagaatcct tgacaagatt
     9781 gaaattatag taaatgattt aataaaatct atttcatatt tgaagtattt cgaaattatc
     9841 ttaattaaag gagcagacta aattgaatga ttacacaccg acacattaca atgagtcgac
     9901 catcctcgaa ttcatcattc aaaacgccga ggtttctcga tcaaaattag cgaaattaac
     9961 aaacttaacg aagtcgtcta tttccgatat cacaaaaaca ttgattgaaa aatcactcgt
    10021 ctacgaatta aacactggaa tggcctccgt atcaggcggt agaaaaccta tttacttgag
    10081 ttttaacaac gaatgcgccc ttgttattgc gatagagatt gggaaggact atatccatag
    10141 ttctttgtcc tacatcaatg gggagttggt caataaattg agttaccatt ccctcttagt
    10201 aaccaaggat acacttgtca tgttgttgac aaaaataatc tccgatttaa cttcgaaaat
    10261 accccagacc ccttatggta ttttaggaat tgggatggca atccatggca tcgtgaaaga
    10321 tgatacagtc ttgtttacgc cttattcaga cattgattcc attcctttga aagacctact
    10381 tgccaaagaa attccctacc caatcgtctt aattaatgag gcaaacgcgg gtgctcttgg
    10441 ggagtatacg tttaaaaccc cttcggagaa tttggttaac atcaatattc aagacgggat
    10501 tggggctggt atagttgaga atggtaagct gtttatcggg gcaaacggca cagcagggga
    10561 aattggacac actacccttt accccgatgg cattctatgt ccgtgcggga atcatgggtg
    10621 tttggagcat tatgcttcta cccgtattct gttgaagaag ctgcttgatt cagatcataa
    10681 accacatccg gacatctcag taatcgttaa aaaatggcac cagggggatg cacataccca
    10741 gaaaatactg aagcataatg cgaaattact cagcatcggt attaacaata ttgtgctaat
    10801 gtatgaccca gaggttgtca tcatcaacag tgaaatttac cgaggcattc ccgaattgat
    10861 tgacgagatt aagaagcact tgactggtaa aaacacaaaa aacttagcta ttaagaatac
    10921 atctctaaaa ggtaattcta cactctatgg ctgtgtttcc atagttgccc agtcctttct
    10981 gaaaatcaat aatctgaaat ttacagtgta aagtgataga aaaatatcct ccttaaaact
    11041 tcctattttt cgggaggata tgattttatt agggtgtaag tacttagcag catttttttc
    11101 actaaatgat ttcagctaac gtttcccaat aacttttatc atttgatttt ttagaaaaag
    11161 cataaatttt gttttttact tccgtcatat tatgatcttt tatttgaata acgctctgat
    11221 tgaaagtagc tatactgtca atatgctgaa tatttaaatt taacactaat ccttcccctt
    11281 tttttgttaa aaatgaaatc cgatcattat ctacgctata agttccttct tttaattttg
    11341 aatgaggatt tgtttttagc tcgttatcat aagcgttagc aaataacgta acggttatag
    11401 gtttatttct cattttttcg tctctccttt ttggtctaga gttttacaac ttttttcttg
    11461 gcgtttgtca agaagcctat cttttgatct tattttttat ttttttaatt atcttctgtt
    11521 ctgttcatac agttgactcc tttcttattt agtccttttt tcaagtatgt ccgtctgtcg
    11581 cctgttttgc ccttatcatg agggatttag ggaagtaaag ggcgagtgta gcgagtgcat
    11641 tttacccttt acttccctaa agaacttatg atataaaccg gttggcgaga gactgacttg
    11701 aaaataggac gtgaatagaa gtaataggtt atgaacaaac gggtaatttt ttcaataaaa
    11761 aaacgaacaa aagcagcaat ctttttcgat tgctgctttt gttcgtttca tctgttttaa
    11821 ttgcatttat ttttgggtat tttgaacccg tggtgctagt taattttaat atcctaaaga
    11881 atatctcaaa gttccttcaa aacgtcgttg tgctcgactt aatagaacac tgtaagccag
    11941 taatatgctt tcaaatctac tctctagccc tttaacagaa cgtgagttga aattt
//
LOCUS       DANKEF010000072        11676 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000072 DANKEF010000000
VERSION     DANKEF010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11676
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.072"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            544..1107
                     /locus_tag="PWZ48_002356"
     CDS             544..1107
                     /locus_tag="PWZ48_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2261994.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1386..1673)
                     /locus_tag="PWZ48_002357"
     CDS             complement(1386..1673)
                     /locus_tag="PWZ48_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2261995.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPNLLCGTHENFYEQLKKIR"
     gene            complement(1663..1992)
                     /locus_tag="PWZ48_002358"
     CDS             complement(1663..1992)
                     /locus_tag="PWZ48_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2261996.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2297..2626
                     /locus_tag="PWZ48_002359"
     CDS             2297..2626
                     /locus_tag="PWZ48_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2261997.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="PWZ48_002360"
     CDS             complement(2913..3398)
                     /gene="vanZ"
                     /locus_tag="PWZ48_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL2261998.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3551..4462)
                     /locus_tag="PWZ48_002361"
     CDS             complement(3551..4462)
                     /locus_tag="PWZ48_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDL2261999.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            complement(4890..5498)
                     /locus_tag="PWZ48_002362"
     CDS             complement(4890..5498)
                     /locus_tag="PWZ48_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL2262000.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
     gene            complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="PWZ48_002363"
     CDS             complement(5504..6535)
                     /gene="vanA"
                     /locus_tag="PWZ48_002363"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2262001.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            complement(6528..7496)
                     /locus_tag="PWZ48_002364"
     CDS             complement(6528..7496)
                     /locus_tag="PWZ48_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2262002.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            7828..9120
                     /locus_tag="PWZ48_002365"
     CDS             7828..9120
                     /locus_tag="PWZ48_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2262003.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNAYGFRSFYNFKLRIMIRHGNALIFN"
     gene            complement(9218..10372)
                     /locus_tag="PWZ48_002366"
     CDS             complement(9218..10372)
                     /locus_tag="PWZ48_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2262004.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            complement(10350..11045)
                     /gene="vanR"
                     /locus_tag="PWZ48_002367"
     CDS             complement(10350..11045)
                     /gene="vanR"
                     /locus_tag="PWZ48_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL2262005.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            complement(11259..>11676)
                     /locus_tag="PWZ48_002368"
     CDS             complement(11259..>11676)
                     /locus_tag="PWZ48_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262006.1"
                     /translation="QEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLD
                     LSEDNPYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMN
                     YAVKLYKEGNMTVNQICEITNVSRASLYRKLSEVNN"
BASE COUNT         3492 a         2359 c         1994 g         3831 t
ORIGIN      
        1 ttaaaacttt tttagcagat tttaaagttt cgtcaaaact aatatattct ggtagctgag
       61 cattcaaata gctttcagac ttcagaagaa aattacgagc atttttatag tccatctcta
      121 ataaagttat tcttttttta tccaatacat tcagctcaat tccttataat attctattcc
      181 ttagtatctc aacattattt actttataaa acgaataaat tttgtagttc taattcaaat
      241 attgcatgtt atatctaata tgggagcatt ttataatttt cattgtgata aactcgttat
      301 taactttagg atacactagt taggattaaa ttaggtatct agaaatttac ttaagatttt
      361 tttgtttatt tcgtatacac actcgtatca aaaacaatcg attatgagac aaaatgaaat
      421 gacctctttt attaaataaa agaggtcata ttttgtatca taacttgttg cattaactat
      481 gtatctataa ctccctttta tgatacaatc aagccagaat taaagtacga aaggaagaaa
      541 agcatgagtg gatacattta tggttatgcc cgagtgagta cacgttcaca agaactaaat
      601 cgtcaattag acttactcaa tgaacaaaat tgtaatgaaa tcctaactga gaaaatgacg
      661 ggaaccaaag cggttcgtcc ccaactaaat cgtttaaaag ataaaattcg tccgggagat
      721 acggtaattg tagaaagttt ttctcgattg ggtcgtagta caaaggattt gattgacctt
      781 gttacctatt ttgaagaaca ccatgttaag ctggtgagct tgaaagaaaa cttcgatacc
      841 agcacgcctc acggacgctt aatgatgact gtcttccaag catttagtca gttcgaacgt
      901 gatttaattg ttgaacgtac caaagaaggt ttgaaaagtg cacgtgcgag aggtcgtaaa
      961 ggtggccgtc cacgagtgaa ccagaaagat gttgatcgag cagtaaaatt gtataaaagt
     1021 caggtttata gcgtaaaaga aattacggaa atgactggta ttagcaaggc aacactctac
     1081 cgctacttaa aagacaatag ggaataatct attttaatct ttaggaatct ataatattta
     1141 tgggagaatt agagtgattt ttttattatt actaaaaaac aatataaaat aacgcatttt
     1201 aagccctctt tgtgcataag gatgaagctt ttaatttgaa agaggcttaa aatgcgtttt
     1261 aattttttct atttttcata aatatatagg ttagtggttt aaaattatag ggtattacaa
     1321 ctctcagctg ttaaaaatta tcttttaaag tgtcgtctat cctctcaagt tgaaacattg
     1381 ggttcttacc taatcttttt taattgttca taaaaatttt catgcgttcc acatagtaag
     1441 ttaggtatca ctgcattatc tttaatttca tatgcaacgc gatatgtcgt acctgcatac
     1501 ttgaatccaa tagcccaaat tcctgataaa tcacccgttt tttgttctcc cgaatgagga
     1561 cgaactgcta tctcatcata aattatcgtt aaaaattgtt cttttaatgt tttctctttt
     1621 aattttttta gatactttat tacgtgttta gaaggtagaa tctcataagc caatttctgc
     1681 tgccaactcc tcttttgtca tcggtttaga atttgcaaca gtatcttcag caatagattt
     1741 aaatgcagaa gttaacgcat tttttctatt tctaaattct tttaagatct cttgtttgct
     1801 ataaccttct gaaagaatat cagccaaaat atcttcacta aaatcaaaaa attctttttt
     1861 aggctctaca aactcataaa ggataccatt ctctacaagc ttagcttcta cttcaacacc
     1921 attgggcaca tcgaattctt tagggaccgt taacataata gaatttcctt gttttcttgt
     1981 ttttgtaatc atattatttc ctccattatt ttacatttcg taattactta gtaattacat
     2041 tgtacgctat ttttagtaga aatacaaaat aataaaactc ctttagaaca attttcaaag
     2101 gagttttatt tgattaggaa attatgtaga ataataatat gtttttaact tggaatcaaa
     2161 aacgcacttg caagtaagtt tacttcaata catgaaattg cttataatag tgtattggaa
     2221 ttaaggtaga ttgacaaaag agtgatcaaa taggtatact tcattcaaat aatcgtttga
     2281 atgtgaggtg agatgaatga aaaaagtttg ccaagtgact gtcgtacata aagaaaaagt
     2341 aaatggaata aaaaataaat tggatcaaaa agatttttca aacttacttg tgttagggaa
     2401 atgtttcagc gatttttcta gaattaaaat tttctatgct ttagaaacct ataaggaaat
     2461 gtgcgtctgt gatttagcag aaatacttac tgctagtgtc gctacaacgt cacaccattt
     2521 acgtttttta aaaaaacatg gaatggcaaa atcacgtcaa gatggaaaag tagtttatta
     2581 ctctttagcg aatgaagaca tcctttccgc tattcgtgtt ttttaaagtt atcagaaaat
     2641 ctatctatga aatcttagtt tgggggtacc gtcaggaaaa tgcggattta caacgttaag
     2701 gaaatttatt aaagagacca attcctcagc actaaaattg ataattgggc tttctttgtt
     2761 actatagtgt taggtaagct ttttacttta tcaccggttt tcccctcact tcacacctac
     2821 gggcgagttt ccccacatcc cgttttccat ctaatccaat tcattcaata tctttatgtg
     2881 tatgaaagat cagattgctt gatttagaca tcttacacgt aatttattct taaatgggta
     2941 cggtaaacga gcaataatac gagcaaaagt atacctacaa aaataataac tctgtctaat
     3001 tttttttgat tcatatgctt attgcttaaa ccatataatt tcagtccaag aaagcctcca
     3061 acagtatttg taattacatc tgttatgtct gtcgctccaa tagcgaagat aaattgaatt
     3121 atttcaaaag taagacttaa aaccagtaca aaagcaaact taggtaaaaa tccgatttct
     3181 ttaaaattga cattcaaaag caagccaaat ggaataaaga ttataacatt atctatcatc
     3241 tctctgaaat tcccagtagc agtaaatgga gtcaagttaa gacttctttg atgataatta
     3301 aatactgata aaatattgta ttgtaatttg aataacacta accagattag tgtcactaaa
     3361 tataaagcta gcaatcctct agataatatt tttcccaata taatttcctc taattctttt
     3421 gtttaagttg tatcattagt atagcaatgt cctagttaac gtgggtttta aattaaaaag
     3481 ctaacgttac cccaaaagtg ctagtacaat aatgctgttc attaatttcg ttggtttcat
     3541 tccgccatcc ttacctcctt gaattagtat gtgttgatcc gggaaacact gtcacaatta
     3601 caccgtctat attgtttcct gatatctcat aacgaagatt agtcggcaca tgaatggtgg
     3661 tatttttagt aacaggataa taaaagatct catatttttc cccatttaca ctaacagaaa
     3721 tggttttttc ttcttttagg taatccatat attcctcgag aacgaaattc ttttctttca
     3781 taatcgcact atgtggtaaa ccaacatagc gaatatgcca tggttcatat tgaattcctg
     3841 ttaactctgt tttgtcctct ggataacgta aaatgaaccc gtatttccaa gcattttctt
     3901 ctatccactt tccttcaggg gctcgttcca ttttcgtcaa gcttgatcct acatctagtg
     3961 ataaacctga attatgctca ctataacctg ctggtaaggc atactcagcc cccatttctt
     4021 ggtaaagcac actttgctca tcaaagtctc gatagccact attaataata aaatgactaa
     4081 cgccaccctt tacagcatca ttgaccatct ctgaaaattt ttgtgctatt tcttttgaca
     4141 tataaatatt actatcaagc aacccgtatc catttattaa ttcgtcatgt ttagataaat
     4201 tcacgatatc tgacttcaca ctttcttggc gaacaggata tttactattg attaatagca
     4261 gatttccttg ataaacctgt tcttctgtaa ttgttttctc ttgggtattt tcagaagtcc
     4321 cactattttc taaatgttct ttgggatttt gatcataatt ttgaaattcg actttttcct
     4381 gagaaaacag tgcttcatta acgtagtcat aacctaagta tattaagaat aacaataaca
     4441 ataaaaaaaa caacttcttc attttcagtc tcctccttaa ctatatagtt ggaagtatga
     4501 aatatttcta gaatcttccc cttaaacggc acaatctcgt ttgagattgt gccgttttgc
     4561 cccgtaaatt acagtcaatt atttcgccct actggattta ctattatcac caatgtagat
     4621 gaattttcat aagtgatacc catgctgaat ttaacgaggc tgaactgcct gttgacaatg
     4681 ggcggtgtga aataccaccc ctctatgcga tcagtcttat tatcaggcta tcttgccgca
     4741 gcgcacgcag ataatgaccg caatatcgct ctgccttaca tcttcaatac tatctactat
     4801 atataaattc cctaagaacc agttacatac gtcgggtttc ctgccgaaag agttagctta
     4861 tatagtttgt ccgtgcaacg gttaaaagtt tatttaacgg ggaaatcaaa atagctattg
     4921 gggtatggtt cgtctcttaa tacatagtgc caccattcga ggctatatgc ttcaaaccca
     4981 ctgttttcca tgatggagcg caaacgtctg cgattttgcg cttcattgca tgatattcca
     5041 tttgccgcat gatgagagcg ttcatccata aaatcaaatc ggctccccat tggtacaagc
     5101 tcacccgtgt ctaatcgata aagcgtaaga tcaatggcac tgccgcggct atggcttgat
     5161 tttgaagcca cgtatccttt tgaaatcatc tcagttcggt caatattggg ataataactt
     5221 tcctttgtca ggttattttc cggctgtgca gcccattgca taaaacagtt tacagcacga
     5281 ttaggacggt aaccgtccca tagaagcaat ccgtaccctt gggtagcagc cagttctttt
     5341 gccttcaaaa gcgattcagc caactcgtat gtccctacaa tgcgatttac ttcataaccg
     5401 tcaaccggtt ttccggtgaa attatcccaa gtggcatatt tagcgtccca acgaacaccg
     5461 tgtactattt catctaaaaa agtaaatcct atttccatgc ttatcacccc tttaacgcta
     5521 atacgatcaa gcggtcaatc agttcgggaa gtgcaatacc tgcagcggcc atcatacggg
     5581 gataacgact gtatgacgtg aaaccgggca gagtattgac ttcgttcagt acaatgcggc
     5641 cgttatcttg taaaaacata tccacacggg ctagacctct acagccgagc gctttatata
     5701 ttttttttgc cgtttcctgt atccgtcctc gctcctctgc tgaaaggtct gcgggaacgg
     5761 ttataactgc gttttcagag cctttttccg gctcgacttc ctgatgaata cgaaagattc
     5821 cgtactgcag cctgatttgg tccacctcgc caacagctaa cgcggcactg tttcccaata
     5881 ccgcacaacc gacctcacag cccgaaacag cctgctcaat taagattttg ctgtcatatt
     5941 gtcttgccga ttcaattgcg tagtccaatt cgtccgcgct attgactttt ttcacaccga
     6001 aggatgagcc tgaacgcgcc ggcttaacaa aaacaggata ggtaaacgta gctgccaccg
     6061 gcctatcatc tttattaata acccaaaagg cgggagtagc tatcccagca tttttcgcaa
     6121 cgatgtatgt caacgatttg tccatacaaa ttgctgagct ttgaatatcg cagcctacaa
     6181 aagggatacc ggacaattca aacagacctt gtatggatcc atcttcacct gacttgccat
     6241 gcaaagctga aaatgctaca tcaacatggt tgatttcata ttcatggttc tttttaacaa
     6301 gtaatccgtg cattttttta tccggcgaga gtacagctga atagcaattg tcgttttccc
     6361 attccgcgca aggtttttcg cacattttcc atacaccaga tttcgtaatt ccaatgtata
     6421 acggctcgta tttttcttta ttaatgttag cggctatctc tattgcagat tttaccgata
     6481 cgtcatgctc ctctgagcaa cccccaaaca gtattgcaac ttttattcta ttcatgctcc
     6541 tgtctccttt caaaatccaa acagttttta atggtttttt caacggtatc acgcaacgct
     6601 tgctcggtat aataggccgt atgcggtgtg attatcacgt taggcattct ttgaagttta
     6661 agtaaaaatt gattatcaat tggtttttgg gtgcaatcag agtagaaaaa ctcttcctct
     6721 ccttccaata catccaatgc ggcaccgccc agtttcccgt tttctaatgc tttaaccaac
     6781 tcataggtat ctacaagtgg accgcgccca gtattgataa gaaatgctcc ttgcttcatt
     6841 ctctgtattt gttcgtggct gataatatag tgcgtatccg tattgagcgg cacatgaagc
     6901 gtaacgatat cgctattttg cagcaactca tcaaacggta catagtttac ctctatactt
     6961 cggctgcgac tataagccaa cactttacat ccaaatcctc gcagccgctc aataaccgct
     7021 ttgcctatct ggcccgttcc caccacacca actgtcatgt cgctgagtac cttgccacgg
     7081 tcgctgtcca acctgaaatc atgtttttcc acagagcgca caatcgattt tacgttgcgt
     7141 actgccataa gaattagcat catagtataa tcggcaacgc tatccggcga gtacgccaca
     7201 ttgtcgacag tgatgcccat tctcttagca gcagttgtat ctatatgatt gcagccgatg
     7261 cttcgggtag aaatatattt cacaccggct ctcttcagcg caagaagaat agaggcggaa
     7321 atctctgatt tatgtcccac actgatacat tgattgaaag gcgcggattt ggcgttggat
     7381 tccgacacgt tggcgttaat tatcgttgcc ataacgccaa agcgaggcga aagagcatgg
     7441 aatgcatctg cctcatcctg ctcacatcca taaacagtaa tgccgatgtt attcataatt
     7501 aagaccaacc ctttctgtga aaggcaccaa ataaaacggg gatgccaatg gtaatcttgt
     7561 caaatctttc tctgaagaac gaaaacggct cgttcttacg gtattaagtc taccgtgtaa
     7621 gaacgagccg tttcttaata ttaaattgtt aatttcctaa gaaaaagtaa agataacctt
     7681 gagattttcc taaaaaatta tggctctata ctttttgatg ttggtggatg acaccagcat
     7741 cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga attaatttgg
     7801 cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa agaaacatta
     7861 gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga aaaatacaaa
     7921 gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga atgcatccac
     7981 tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt ccggttgtta
     8041 atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg cttcaaatgc
     8101 aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca ttgtttcatc
     8161 agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc aatgacagag
     8221 atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa gcgattttat
     8281 cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga tgaattcaaa
     8341 tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca aacacagcgc
     8401 ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt tagtcatttt
     8461 tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc tccttatgta
     8521 tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt tcacatcgtc
     8581 caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca actattaaaa
     8641 agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg gaaacttctt
     8701 caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg cttcaaagct
     8761 catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc tttgcgtgta
     8821 ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta cgtatcgttt
     8881 gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac cttgagaacg
     8941 ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc aaatggccca
     9001 cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgcatatgg ctttcgtagc
     9061 ttttataatt tcaaattgcg catcatgatt cgtcatggca acgcattgat tttcaactaa
     9121 aaaagatcaa gaggaactga tccacttgat ctccaattat tctaagattt ctctttacca
     9181 acatcatttg acaaagagcc aaaattatat acatctctta ggacctcctt ttatcaacca
     9241 agtctggcat cgctggaagc tctaccctaa acgtcgtata gttatcattg ctttccgcgt
     9301 aaatctgccc tccatgctga acaataattt cttttgcaat cgccaatcca agtcccgcgc
     9361 cacccgtatc ggaagaacga gcattgtcca gcctatagaa cttttcaaat atggcagcta
     9421 gcttatcttt tgggatgctt ccagtgttct tgaattcgat tgacaccaca tccccggaga
     9481 ggcccgcggt aatgtcaatg atgctgttat cctcactgta tgcagcggcg tttttcaaaa
     9541 tgttgttaaa gactctcgcg agtttatcag ggtcgccgga cacggtcaga tcctcggggg
     9601 cgtgaataac cgcctgtttt ccatgtgcgg aaagctgagg ataaaattca tcggtcatct
     9661 gcaccagcat atagtatagg tctatgtgcg tttttgttag cgttatcgtt tgtaggttat
     9721 accgtgtaat ctcaaaaaac tcgtcgatta gctgttcgag tcgatacgct ttgtccaacg
     9781 tgatatgcac atactttgcc ttttgatcta ccggcatgtc tggagcctcg tcaagcaggc
     9841 tcaaataacc gataatggat gtaaggggcg ttttaatatc gtgcgccaag tacataacaa
     9901 cgtcattttt tctttgttcg gccagctttg catcctgctc tcgcttttcc agagtccgtt
     9961 ttaatgtgtt gagcttttgt tccataacat ccatttccgc agaaagctca atttgtttat
    10021 cttcgttctg aataagtaca tcaatgccgg tatttatctc gtcaaagtat tttgcgaatt
    10081 ttgaaagcat gacgcgacat agaataagaa tactaatgac aatcgccaca taaataaaga
    10141 tatctatatt gttccgtatg gaatattgat ataatttcat cgcgtccagg tgatttaagt
    10201 catatttgtt ttccaaaata cttaagatcc aatccccaag tttccctcgg atcattgaac
    10261 gaatatacaa cacgaataca attgctacca caacaattgc aacgatatac atgtaaagtt
    10321 ttcgttctag tttggaatag tcgttttttt tatttttcaa ttttataacc aaccccccat
    10381 accgttttta tatatttcgg attatcaatg gtgtcgttca ttttttcgcg caaatgccgg
    10441 atatgcacgg tgatggtgtt gttgctcttg ctgaaatatt cgtcgcccca tatctcatga
    10501 aatagcagct cggagctaac cacattcccc ttgttttcac agaggattcg cagtattgaa
    10561 aactcggtgg gagtaaggga taactgcttc tcgttcagat aacactcatg ggtgttaaca
    10621 ttaatgacaa ggccggagtg gacgataaca ttttcgttct gctcctttac tccactgaat
    10681 tttttgtatc ggcgcaactg ggcctttacc cgagcaatta actccagtgg gcgaaagggc
    10741 ttcgttatat aatcatccgc gccgattgtt aaccctgtaa ttttatctac ctctgtatct
    10801 ttcccggtca gcatgataat cggataggtg tgcttgtccc ttattttttg acagatagta
    10861 aggccgcttg tgccgggaag catgatgtcc aatatggcaa ggtcaatctc agacttgtct
    10921 atacattcca atgcttcttt ggcggtatag tatttgaaaa ccgtataatt ctcgtttttt
    10981 aagtataatt caaccaaatc ggcaatttca tgttcatcat ccacaataag tattttatcg
    11041 ctcatagtta tcaccctttc acataagttt gcccttaata atttatcaga ttatagggcc
    11101 gaatttataa gcatttacat aataaatgac taagaatttc ttaagatgat gattcttttt
    11161 ttatcaaagg gcttttcggc atcgctagga ggctgttaat attttatagt ttccttttct
    11221 tctttaaaaa tattgcccat tagcggaata cagaatggct aattattcac ttctgataat
    11281 ttcctgtata atgaagccct agatacatta gtaatttcac aaatttgatt tacagtcata
    11341 tttccttctt tatatagctt taccgcataa ttcattcctg cgtgattttt atgatacttc
    11401 tttaatcgac ctttaaactt tccttctttc ttagccaatt caatcccttc acgttgtctc
    11461 atccgaataa gatctcgctc taattggtta acaccagcca ttacagtaat taagaattgg
    11521 ctgtatggat tatcttctga taaatcaagc catgtatctt ttagtgattt taaacttgcc
    11581 tttttatctc gtatgttatc gattaattca aatagatctt gtgtactacg agtgattcga
    11641 gttaagtctg taacataaat gatgtcatct tcctgt
//
LOCUS       DANKEF010000073        11150 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000073 DANKEF010000000
VERSION     DANKEF010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11150
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.073"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            6..305
                     /locus_tag="PWZ48_002369"
     CDS             6..305
                     /locus_tag="PWZ48_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2262007.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="PWZ48_002370"
     CDS             378..1739
                     /locus_tag="PWZ48_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDL2262008.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFLSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLLFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGILTMPIYLYGATYLAPILSDSAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="PWZ48_002371"
     CDS             1761..2021
                     /locus_tag="PWZ48_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262009.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="PWZ48_002372"
     CDS             2035..2787
                     /locus_tag="PWZ48_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HDL2262010.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="PWZ48_002373"
     CDS             complement(2881..3486)
                     /locus_tag="PWZ48_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDL2262011.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIEQAREWTDHLLTK"
     gene            complement(3542..4516)
                     /locus_tag="PWZ48_002374"
     CDS             complement(3542..4516)
                     /locus_tag="PWZ48_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HDL2262012.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DCPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4894..5613)
                     /locus_tag="PWZ48_002375"
     CDS             complement(4894..5613)
                     /locus_tag="PWZ48_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2262013.1"
                     /translation="MPKYEEIANILRERIRSKEYPPDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5740..7011)
                     /gene="celB"
                     /locus_tag="PWZ48_002376"
     CDS             complement(5740..7011)
                     /gene="celB"
                     /locus_tag="PWZ48_002376"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HDL2262014.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7164..8561
                     /locus_tag="PWZ48_002377"
     CDS             7164..8561
                     /locus_tag="PWZ48_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HDL2262015.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWNHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSRLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKAIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGTIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8642..9082
                     /locus_tag="PWZ48_002378"
     CDS             8642..9082
                     /locus_tag="PWZ48_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2262016.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9229..9555
                     /locus_tag="PWZ48_002379"
     CDS             9229..9555
                     /locus_tag="PWZ48_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2262017.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9556..10305
                     /locus_tag="PWZ48_002380"
     CDS             9556..10305
                     /locus_tag="PWZ48_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262018.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10331..10741)
                     /locus_tag="PWZ48_002381"
     CDS             complement(10331..10741)
                     /locus_tag="PWZ48_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HDL2262019.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNK"
BASE COUNT         3538 a         1948 c         2291 g         3373 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttcgaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcctgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtctta ttgctgcttt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattacca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcctc acgatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgattcagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaaggaccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcaaaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgggagat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc tcgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccaatc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa aaatgaagta gcatgaagag
     3541 attattcacg ttttttattt gcatcacttg tatcgaaagg acggacatag gcttcaatgg
     3601 cttcacgacg ttcttctaag aatggaggaa gagatagttt ttctcctgct gattctgctg
     3661 gttcatcttc aaggaatcca ggacaatcag ttgctaattc aaacagtacg tgatgagcgg
     3721 ctaggaagta ctcagattgg aaatagaaac gatcgacgaa accagaattt ggcaattcaa
     3781 tcttattgat tacttgaatc cagtattcca atgcttcttt gtctgctaca cgcaatgcta
     3841 agtgatggat atttccaaat ccttcgattg cttttggcaa gtctgtccga tgttcaataa
     3901 tgatgcttgc accatttccg ccttcaccga tttcaaataa gtagaaatct cctgaatggt
     3961 ctgtttcttt gaatcctaag attttttcaa gaatcaagcg gatatgctct aattgactga
     4021 cacggacaaa gacaggtcca agtcctgtga tcgcaaactc tgtcggtaca ttgctgtttt
     4081 tccaaggaat gccagctgct acaccttgat tgttttcgtc agagatcaat tgataatact
     4141 gattatcgaa atcttcaaat tcgatatatt ttttgccgaa acgttcattg attgctgaat
     4201 gagtaacgtc atatttttca aaacgttcca accaaaagtc taatgctgcg tcacttggca
     4261 cacggaaaga agcacgagag atcgtatctg ttcctttttt tccttttggc tgatttggaa
     4321 aatcaaagaa agtcatatct gttcctggcg caccagtatc gtctgtaaag taaagatggt
     4381 aagtttcgat gtcatcttgg ttaactgttt ttttgattaa gcgcattcct aaaatatctg
     4441 taaaaaagcg gtagattttt gtagcgctag atgtcattgc tgtgacgtgg tgaattcctc
     4501 tgatttgtgt atccatgata aatttcctcc tgtcaactta caaaaagtaa gtagttgatt
     4561 tagacatagt atagagggtg catctctatt ttgcaattaa tttgtcttct aaagtatcag
     4621 gaaggaatca agaatggaaa agaggaggga cggatcaata aaataaaaag acttacgact
     4681 agctgtatcg atttcaaaaa aactagcttt aaaaagctaa ctttagaatc attacgggtg
     4741 gtcataagtc tttttatgct gagttatttt ttcatttttg agctaatgct taggatagga
     4801 tagaatgttg atttcacgat ctcttcttgt tacttgaaac tgcttactac tgtcacgatc
     4861 tcttggtaaa cggctttttc acaacctctt catttaagtt gaacgcacat ccagcacaga
     4921 atagttccga acatcaaatc ggtttcggct ggaagaatat tcaatcggct gtccatcttt
     4981 gagatagacg acttgctgaa cttccagtac aggatcgttt tctgcacagt tcaaatattg
     5041 ctgatcatat tcatcacttt tatctgcaga aatagcacga taagcccctg caaaatggac
     5101 attcaattct tgatgaagat gatcatagat agaggctaac aaatgttctt tttttaaacc
     5161 cggcacaaga tggactggca taaaagtatg ctctaaaaca tatggctcgc catctaagat
     5221 ccttaaccgg tgaatattgt agacaggttg ctcggcatcg atcatcaatc gttcttggat
     5281 atattgatta gggaattcaa catcaaaatg aatgatttga ctcttcaaag aacgatgtct
     5341 gcgtttcatt tggagactca aaccttcata ctcgttagcg ggcgatgtgt ctttttgcat
     5401 aaatggatga ttcaagattt tcgttcctga tcctcgctgc gaatagacaa gtccttccat
     5461 tgctaagata ttgatggctt ttttgattgt catccggctc gcaccaaatt ctttgaccaa
     5521 gtccacttga ttaggcaaaa aactatcggg aggatactct tttgaacgga tacgttctct
     5581 taaaatattt gcgatttctt cgtattttgg cacgttattc acctcttcta gagtataacg
     5641 aaagattatg cttccgacaa ctcgaagtag catctggaac attgtttatt cgaaaaaaga
     5701 ggtcgtgaca agtttttgtc acaacccctt aatgatctat taagcaatat ctaaatctgg
     5761 ttgtttatct agtactttca ggaatgggta ccagataaag gcaacgatga caaagtcgat
     5821 gacttgcagc ataccaccaa gccatgaatt tgttgctaag acaccgcttg caatgattgg
     5881 cacagtccat ggaacagaga cgccagttgg tgcagggaca agtccagcag ccattaccaa
     5941 ataattgaac gtcgttacga ttagaggtgc cacaacccaa ggaataagga ttgttgcatt
     6001 caatacgatc ggtagaccga agatcgctgg ttcatttaca ttgaaaatac caggtgctaa
     6061 agctaatcgt ccaacatcac gatactgttt ctttttaaga acaaaggcca tcaataaaac
     6121 gacagctaaa gtcattccag agccaccaat accaacagtg aaggtttcca tgaatggttt
     6181 tgtgatgatg tgtggtaatt cttttccatg ttgataagct tctaagttat caagcatcag
     6241 tgtattccaa atcgggtcca ttacagagtt gacgatgatt tgtccgtgaa gtccgaagaa
     6301 ccataagaat tggacaaaga acaaagcaat caatgttgca ggtaaactac ttcccaaacc
     6361 agttaatggt ttttggatca cttcataaac gacgtcatgt aagttagcat tgaaaatacc
     6421 agtcatcaca gcattgatta ctaagaaaat agttagtgtc actacagcag ggatcaaagc
     6481 ggcaaatgat tttgttactg caggcggaac tccatcaggc attttgatct gccagccgcg
     6541 tttcgtgatt ctgcagtaga tttctccagc caagaaagca gcgatcattc caatgaacat
     6601 tccttttgcg ccaagacgat caagtgaaag cacaccagtc acatcttcac cgccagcggt
     6661 tttcattgca aaaggcgtaa gaatcaagaa gctggcaaaa gacacggcac caccgaaaat
     6721 accttcaacg tcataagatt ttgataaata atagccgata ccaaaagtaa caaaaaccga
     6781 catgatgctc attgttgcat tttgcccatt accgaataag ttcgataaag ttccctttgt
     6841 tgcgtcactg aaaaaaggca agttattgat aacgacaacg atcgaaccga acatcgttaa
     6901 agggaaggaa agcatgaaag catcccgcag cactgtaagg taacgatttt gccctaataa
     6961 attagctaag ggcatgattt tctcagaaag cttgtccata aaaccgttca tttttattca
     7021 cctcaaattt atttgcaatc gattacatac aaagtataac gccaaaaatt ttaaatgtct
     7081 agtcattttc ataaaaagta ttgatttatt ttttagggtt cgttatgatt acgatatcaa
     7141 gaaaaaagag aggaagtaaa aaaatgaaaa cctacacatt cccagaaaat ttttggtggg
     7201 gttctgctgc tagcggaccg caaacagaag gaagagtaag tgatgatggt aaaggagaaa
     7261 atatttggaa tcattggtat gaattagagc ctgaaaaatt cttcgatcaa gtaggacctg
     7321 aaaaaacttc gcaagtttac acaaaataca aagaagatgt tcagttaatg aaagaaactg
     7381 gccataacac atttcgtaca tctatccaat ggagtcgttt gattccagaa gggacaggtg
     7441 aaatcaatca aaaagctgtt tctttttatc gagattattt tactgaattg ctggataatg
     7501 gtatcgaacc ttttgtgaac ctttaccatt tcgatatgcc actaccactg caagaaaaag
     7561 gcgggtggtt atcaagagaa acggtcgacg cttatgtttc ttatgcagca gcgtgctttg
     7621 aactgtttgg agatctggtc aaaaaatggt ttactcataa tgaaccaatc gtacctgtag
     7681 aaggaggcta cttgtatcaa ttccactatc caaatcatat caatatgaaa gaagcggtcc
     7741 aagtaggcta tcatgagaat ctagcaagcg caaaggcaat caaagcctac catgaaggcg
     7801 gacatgatgg tgagatcgga atcatcttga atctgacacc aagctatccg agagatgaaa
     7861 cgaacccaga agatgtaaaa gcggcgaaga tcgctgacgc attcttcaac cgatcattcc
     7921 ttgaccctgc agtgaaagga gagtttccac aagagttgat cgaaattgtc aaagaattgg
     7981 ctataatgcc agagatccat gaaggagacc tagaactcat ccgtgaaaac acgattgacc
     8041 tgctaggaat caattactat cagcctcgtc gaatcaaagc aaaagaaact ccaatcaatc
     8101 acgaacatag ccccatgcca gatgattatt ttgataatta cgacatgccc aataaaaaaa
     8161 tgaatccata tcgtggatgg gaaatttacg aaaaaggaat ctatgatatt ttaacgaata
     8221 caagagaaaa ctatggtaat atcaaatgct atatttctga aaatgggatg ggggtcgaag
     8281 gagaagaacg atttgttaac agcgaaggaa ctattgaaga tgattaccgg attgagtttg
     8341 ttacggatca tttgaaatac gtccatcaag caatccaaga aggaaccaat tgtgtgggtt
     8401 accacatgtg gacatgtatg gacaactggt cttggacaaa tgcttataag aatcgctatg
     8461 gctttatcgc tgttgatttg gcacaagacg gtaaacggac gatcaaaaaa tcaggtcgct
     8521 ggtttaaaac ggtctctgaa aaaaacggat ttgatgcctg atattttttg ataaaacgtt
     8581 cacaaaatgt ttgttctaac actgtaatcg tgctacacta acagtgaagg aggcgtttac
     8641 aatggtagat acttatcgta atattttagt agcaattgat ggttctgaaa aggcagaaaa
     8701 agcatttctt gaggcaatca cgattgccaa acgaaatcag gcaacgttgc acatcctgta
     8761 tgtcaatgaa gtaacaggca attactttgg tgattttgca tttgttacaa caagccttca
     8821 ggaagaattg gatgaggttg cggaaaacca gatgaaagaa catcgaaact tggcaattga
     8881 aaaaggcttg acagatatcg agacatatgt tctttacggt tatccaaaga cactgattgc
     8941 taatttccat gaatcgaaag aaccaatcga tctgattgtc atgggggcga caggtctgaa
     9001 tgcggtagaa cgtgcactag taggttccac tacttcctat gtagtcaacc acgcatcatg
     9061 taacgtcatg gttgtaaaat aaataaatta agggaacaga aagcaaaaaa gcataacgct
     9121 tcttttgctt tctgttcctt taattttttg taaaaaatta ctttacctga tgaagtaatc
     9181 atgctattct aattacaacg acagatggag taatatggag gaaagaaaat gatttcaagt
     9241 gatggaatac gcggatacaa tgatgcgatt attttatcga tccttaacaa aaacgattct
     9301 tatggatatg aaatttctaa aacgattcgt gagaggacaa atgattccta tgtgatcaaa
     9361 gagacaacac tttactccgt ttttgcccgt ttagaaaaga atcaactgat tgtttcgtat
     9421 cctggagaag tgactcatgg aaaaaagcgg acatattata aaatcactga aatagggcgt
     9481 aatcttttag aagaaaaaaa gcaagagtgg ctgacgacga aagacgtagt cgatcaattt
     9541 ttggagggaa attaaatgga tacaatccgt aattatttag acagtctttt tatcggagta
     9601 cctcaaagta ccgagataga taagctgaaa actgatttgt tggcaaatat ggaagaccat
     9661 tatcatgaat tgatgggaga aggaaaaaat gaacaggagg ccatcggtac agtaatttcg
     9721 acatttggtt caatcgatga attattagaa gaactagatg tcgaaaaaaa acatcaagct
     9781 gacgagacgg aaacaaacac agcttctatt tatttaagcg aagcagaaaa ctactggaaa
     9841 gaatacagag cggcaagtct ccaagtggca tcaggtgttc tattcattag cttatctttt
     9901 gcaagttttc ttttcttttg ttcagcaggg tatgtgttca tgggtatttc ttgtctgatt
     9961 ttcggtatag cattagctgt cggttttttt attgcttctg gtatgaaaat cacacgttta
    10021 aatcactttt tgcaccatcg gaaaatacct gaaaaagtat tagcagaagc aaaagaaaaa
    10081 gaagaagagt atcagcgaag ttttggcttc tcactgattg caggaattgg attatgtatt
    10141 ttttcacttt tcccactttt ggcttcttta atgtggtata tggatggtag tatcggtgcc
    10201 tctatatttt ttgttacggt aggaacggga gtcttcttga tcatttatgg cagtctggtc
    10261 cgtcattctt atcgtcaatt cactcaaagt gcttattact ggtgaatgta ttcagatttg
    10321 taaaaatatt ttatttattg cttacccatg taaaatcgtt atagacagat ttcagttgac
    10381 ttctgcgcca ttgtacaggg ataaaaagac gcatactatc ttcattgaat ccgactaata
    10441 atttctttcc gtcagtaatg atgggacgtt gtaacaaagt aaaatgcgag acgatcagat
    10501 cgacaagctc tgaggtcgta cattggtcga tttgatcttt caaattttta taatcaatcg
    10561 agcgtttagc taatagatcg tcaaatccgt tttctgttaa tgacaataaa taaaatatct
    10621 ctttccttgt taaaggctgt ttaggtgtga aaattcgttc tctgtaaggc atttgatggt
    10681 ctttcagcca gtttgccatc agatggtaac tatgtgtgcg agaagaatag atcgtaagca
    10741 taaaaaaact ccttaaatag tccattaatt tgttagatct gttgagataa ataataggta
    10801 aggagctgtg cataagcatt gatcaaaaca acccaaacat cattagaaat aactttctaa
    10861 agtcttaaac ttgcgacgag aaacaactag actaatagaa aaaatgtctt agccatttgg
    10921 cttcctgttt gtttttttgc ttatgctgca gctcctcggg aaagggttct aacaatttga
    10981 agaaggttga tgattgatct agtttaaatt gtgtgaaatt tgaaatctgg tgtttttact
    11041 agatgaatca cctaacaact atgattataa cttatagaac ttctgttttg tacttggttt
    11101 tgttatagtt gttataaagt tttattttat tacagaacat ttttaattaa
//
LOCUS       DANKEF010000074        10977 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000074 DANKEF010000000
VERSION     DANKEF010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.074"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            316..1338
                     /locus_tag="PWZ48_002382"
     CDS             316..1338
                     /locus_tag="PWZ48_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2262020.1"
                     /translation="MIAELKPKYERQNFLVRDDRLDHAVAFLWKDPQTKETVGASYQG
                     TFIDYERFGERGTYKHIDKNSTANHGFNLKIGDPKQIKFFESSIDLLSYAALNRDQLN
                     DTWLVSMEGLKHHVISHYFGEAVSELRKKQAFPQSIEICVDNDRAGHIFYEKEQLMGA
                     VDPFTNQKVRCERGIANDWQVPKEYKIIYEEVAKEEKVTPEAIMAIHKTENNLQLTNQ
                     LVSAHKVNAFFGQQLSVNDSIEAINLKDICREVAKELKVCERVDGTYDFDRFYQEKGD
                     INAQILFSYKAEQYYKGYKNHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
     gene            1349..3553
                     /gene="topB"
                     /locus_tag="PWZ48_002383"
     CDS             1349..3553
                     /gene="topB"
                     /locus_tag="PWZ48_002383"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2262021.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            3569..3718
                     /locus_tag="PWZ48_002384"
     CDS             3569..3718
                     /locus_tag="PWZ48_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262022.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            3793..4386
                     /locus_tag="PWZ48_002385"
     CDS             3793..4386
                     /locus_tag="PWZ48_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDL2262023.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            4399..5298
                     /locus_tag="PWZ48_002386"
     CDS             4399..5298
                     /locus_tag="PWZ48_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDL2262024.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            5301..5798
                     /gene="ssb"
                     /locus_tag="PWZ48_002387"
     CDS             5301..5798
                     /gene="ssb"
                     /locus_tag="PWZ48_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2262025.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            6164..6424
                     /locus_tag="PWZ48_002388"
     CDS             6164..6424
                     /locus_tag="PWZ48_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262026.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            6869..7075
                     /locus_tag="PWZ48_002389"
     CDS             6869..7075
                     /locus_tag="PWZ48_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HDL2262027.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            7123..7326
                     /locus_tag="PWZ48_002390"
     CDS             7123..7326
                     /locus_tag="PWZ48_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262028.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            7342..7779
                     /locus_tag="PWZ48_002391"
     CDS             7342..7779
                     /locus_tag="PWZ48_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262029.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            7772..8479
                     /locus_tag="PWZ48_002392"
     CDS             7772..8479
                     /locus_tag="PWZ48_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262030.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            8859..9038
                     /locus_tag="PWZ48_002393"
     CDS             8859..9038
                     /locus_tag="PWZ48_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262031.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            9363..9626
                     /locus_tag="PWZ48_002394"
     CDS             9363..9626
                     /locus_tag="PWZ48_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HDL2262032.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            9620..9970
                     /locus_tag="PWZ48_002395"
     CDS             9620..9970
                     /locus_tag="PWZ48_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2262033.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
     gene            complement(9994..10608)
                     /locus_tag="PWZ48_002396"
     CDS             complement(9994..10608)
                     /locus_tag="PWZ48_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262034.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDFVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVQLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANAKDPQ
                     RRLVYKNIVEDLKKGVAIAKIAKDYNVTRQTVYRIKKDSMVNHE"
BASE COUNT         3981 a         1816 c         2211 g         2969 t
ORIGIN      
        1 tcatttgttt ttgatcgaag aaaacaacgt ttttattgga acagtcaaaa tattagtggc
       61 gacattattg aattagctaa actttttttc atagacaaag agatccaaga ccctaagcaa
      121 caatttaaag cagccactga ttttattcta aagaatgaag acaaaacaga acgagtggaa
      181 aatcttcatt ttgaaacaga aaagtacaaa gatcaccccg ttgattatca accactcaca
      241 gaaaaaggaa ggaattatct gaaggaagaa cgaaaactac cagagtggct gattgattat
      301 gcagaaaaag aagggttgat tgccgaacta aagccaaaat atgaacggca aaatttctta
      361 gttcgagacg atcgtttaga tcatgcggtc gcctttttat ggaaagatcc acagacaaaa
      421 gagacagtag gggcgagtta tcaaggaacg ttcattgatt atgaacgttt tggtgagaga
      481 ggcacatata agcatataga taaaaattcg acagcgaatc atggctttaa cctaaagatc
      541 ggtgacccta agcagataaa attttttgag agtagtattg atttacttag ctatgctgca
      601 ctgaatcgtg atcagttaaa cgatacgtgg ttagtctcta tggaagggtt aaaacaccat
      661 gtgatcagtc attattttgg tgaggcagta tctgaattga gaaagaaaca agcctttcct
      721 caatccatcg agatttgtgt ggataatgac cgtgcaggac atattttcta cgaaaaagaa
      781 caattaatgg gcgcagtcga tccatttacg aatcagaaag tacgttgtga acgaggaatc
      841 gctaatgatt ggcaagtgcc aaaagaatac aaaatcattt atgaagaagt cgcaaaagaa
      901 gaaaaggtca caccagaagc tattatggcc atacacaaga cagaaaacaa tctgcaatta
      961 acgaaccaac tggtttccgc tcacaaagta aatgcgtttt ttggccagca gctttctgtt
     1021 aacgattcaa tagaagccat taatttgaaa gatatttgta gagaagtcgc aaaagaatta
     1081 aaagtttgtg aacgcgtgga tggtacgtac gattttgatc gtttttatca agaaaaaggg
     1141 gatattaatg cgcagattct tttctcttat aaagcagaac aatactacaa aggctataaa
     1201 aatcatgaac atgaatttgt acctgaagta aaaaaggact ggaacgatca attgaagcat
     1261 gagatccagc aacaagaaat cagaaagcaa aaacgtgcca tgttgtttca acaaggcaga
     1321 caacaagaaa gggagtgaaa tcgtcatgat gaaaaccgtt attttagcag aaaagccgtc
     1381 acaagcgaaa gcttatgccg atagcttctc taaagccact cgaaaagacg ggtattttga
     1441 aatccaagat cgattgtttc ctggtgaaac tgtgatcacg tatggttttg gtcatttagt
     1501 cgaattagat tcgcccgata tgtacgatga aaattggaaa caatggtcat tagaacattt
     1561 gcctattttc cctacacatt atcactatca tgttcctaaa gataagaaaa agcaattcaa
     1621 ggtggtgaaa cagcaacttc aatcggccga caccattatt attgcgacag atagcgatcg
     1681 tgaaggggaa ctgatcgctt ggacgatcat tcaacaagta ggcgccgatc aaggaaaaac
     1741 ctttaaaaga ctttggatca actccttaga aaaagaagcc atttatcagg gattccagca
     1801 gttacgagac gcaaaagaga cctatccaaa atttgaagaa gcacaagcac gtcagatcgc
     1861 cgattggtta attgggatga acggtagccc actgtatagt ttactcttgc agcaaaaagg
     1921 cataccagga agcttttcct taggcagagt ccaaacccca acgctttaca tgatttatca
     1981 attgcaggaa aagattagaa atttcaagaa agaaccttat tttgaaggaa aatccgaagt
     2041 aatcgctcaa aatggcgcat ttgatgcgaa gcttgatccg aatgaaaccc aagcgacgca
     2101 agaagcattt gaagattatt taaaagaaaa gggggttcaa ttgggaaaac agccaggcat
     2161 gattcgccaa gttgaaaccg agaaaaaaag cgcggcaagt ccacgtcttt tttctctatc
     2221 cagccttcag tctaaaatga atcagctcat gaaagctagt gccaaagaca ctttggaagc
     2281 catgcaaggc ttgtatgaag ggaaatatct aagctatccg cgaacggata ccccttacat
     2341 tacggaaggg gaatatgcgt atctgttgga tcacttagac gaatacaagc attttttgag
     2401 ggctgaagaa atttcgactc ctattcatac accgaatagt aggtacgtga acaacaagaa
     2461 agtccaggaa cattacgcaa tcattcctac aaaaacagtt atgacagaag cgacgtatga
     2521 aagtctgaca cctttacaac aagcgattta cgagcaagtt ttaaaaacga cagtggcgat
     2581 gtttgcggaa aaatatacct atgaagaaac aacgatcttg acgcaagtcc agcagcttca
     2641 gttaaaggca atcggaaaag taccgataga tctaggttgg aagaagttat ttggcaaaga
     2701 gagcgacgga aaagagaagg aagaagagcc gctacttcct aaagtgacaa aaggagaaat
     2761 ggtgacggta gatctacaag tccttgaaaa agaaaccaag ccaccacaac cttataccga
     2821 aggtacgttg atcactgcga tgaaaacagc tgggaaaacc gtggatagtg aagaagcgca
     2881 atccatattg aaagaagtgg aaggaatcgg tacagaagcg acacgggcga atattattga
     2941 aaccttgaaa caaaaggaat atatcaaggt agagaaaaat aagcttgtgg tgacgaataa
     3001 aggggttttg ctttgccaag ctgtggaaaa agagccgctg cttacgagtg ccgaaatgac
     3061 cgccaaatgg gaaagctact tgttaaagat tggagaacga aaaggaacac aaaccacttt
     3121 tctagccaat atccaaaagt ttgtctctca cttattggaa gtcgtcccag gacaaatcca
     3181 atccattgat tttggatcga cgttacaaga agtgaaagca gccagtgaaa agcaagaagc
     3241 cgcgcgtcat ttaggaattt gtccaaaatg ccaggaacaa gaagtcttac tctatcaaaa
     3301 ggtggcagct tgtacgtctg aagcctgtga ttttaaatta tggacaacga ttgccaaaaa
     3361 gaaactcaca gcgacacagc tgaaggagat tattcaaaat agccgaacaa gccaaccagt
     3421 gaaagggtta aaaggtcaga agggttcgtt tgaagcaacg attgttttaa aagaagactt
     3481 cactacaggc tttgaatttt ctgaaaagaa aaaaacgaac ttcaagaaaa gaacacgacg
     3541 cacgacaaaa taatggaggg ttaatcttat gcgtaataca acacacaaac aacaagaaat
     3601 cgaaaatcaa aaagccaaaa ttcgttcaga acaagagaaa ttacgtgcca gtaaaagaag
     3661 acttcagcaa ttaaagaacc agcaacggat tcaaaaagaa atcgaattag atcgatagac
     3721 attaaccttg ttacaaatac actatcatga tatatttaat gtagtgtatt tgtaacaaaa
     3781 ggggggaagt aaatgcaaga gaaactgaaa gaattgttgg ctattcgcga tggaaattta
     3841 tctatgaagg aagccagaaa attaggaatc gcagcaacca ctgtacaaag attggtgaat
     3901 caaggaaaat tgatcaaagt tgatcgtggt tattatgtag aggatggtca tggaatagat
     3961 gatttatttt ggcttcagca acgtttttcc aggggaatct tctctcatga aacgacttta
     4021 gatatttacc agctttctac gaatatgccg aaattcattc acttaacttt tccccatgga
     4081 tataacgttg atcgttcaat tacaaaagag cagcagttac aatttcattt cgtgaagaaa
     4141 gaagtttatg aactaggaaa agtagaggga acttcctttc aaggaaatcc tattactatg
     4201 tatgataaag agcgtacact ttgtgatatt tggaatccga gatatgaaat tgaatatgaa
     4261 atgaaattag aagcattaaa agagtacatg gaagacccat tgagagatcc aagtaaatta
     4321 agagattaca tgacaaaact acatgtagaa aaagaaatga aataccacat gcaaagctta
     4381 tattgatgga ggaaaaagat gacaccgaca caatttgcag caaaaacaag aaatttagca
     4441 aagaggacag gattaaatgc acagttagta caacggcatt ttatgatgga tcgtttaatt
     4501 gaacaaattt ctgaatctaa gtataaaaat gatttcattt tgaaaggtgg ctttttatta
     4561 ggatcaaaat atggaatcga tagaaggacg acaattgata tcgacacgac atttagaaat
     4621 agcaaattaa ctgaagaaaa attgaagagt atttttaatg atttaactag gaaacccacg
     4681 aaagaaggta ttgttttctc tattcaagga atgaaggaaa caagagaagc cgatttttat
     4741 cctggatttc aaattaaatt ggtggctaat ttagagaaag tacgtgtgcc atttaaaatg
     4801 gacgtgacaa caggagattc tatttatcct gaagtagata cacactatca ccaattaatg
     4861 tttgaagata aaaaggtgga gattccagct tatccaacag cacaaattat tgcagaaaag
     4921 ctaagtgcta cgttcagctt tggcacagat aattcgagag caaaagatta ctacgactta
     4981 tttaccattc cgaaactaga aaatatcgat acgaaagagt tatacaaaag tgttcgaaat
     5041 acatttacaa aacgtgggga tacaaaaccg ttgtcctttt attatgaaag agaaatggat
     5101 aagatcatag gaaatgacta tttggaaaag cagtgggaaa aatatcgctc agttaacacg
     5161 tttgcgaatt ccatttcatt tacagataca gtcaacgaaa ttgatcacct aatgaaaagt
     5221 gtgatcaaaa ttgaaaatca agaaagaaaa cggcagttta aaagatcaca acaaatgaat
     5281 caagaaatgg agagataaag atgatcaata atgtaacttt agtaggacgc ttaaccaaag
     5341 atccagattt acgttatacc gcgagtggta ctgcagtagc gacttttacc ctagctgtca
     5401 accgaaattt cacgaatcaa aacggcaatc gagaagctga ttttattaac tgtgtgattt
     5461 ggcgcaaacc agcagaaaca atggtgacat tagctaaaaa aggtagcttg attggtgtag
     5521 ttgggcggat tcaaacgcgg acttatgaca accagcaagg acaacgtgtg tatgtgacag
     5581 aagttgtggc cgataatttc caactattag aaagcaaagc agcgacagaa agccgtgcgc
     5641 acgctgatca atcgtccacc agtccaagca ccaccacttt tgaacaacgg gatacagcga
     5701 cgcctaataa caatggattg aacgctttac agaatccatt tgggaatcca tttggtggac
     5761 aatcaatcga tattagcgat gatgatctac cgttctaagt agcattttct attggttgca
     5821 tttgctacgg tttatgttat aatttagtca acaaaaagaa ttttcaatct ataaccaaaa
     5881 accactagct ggcgggctag tggttttctt ttacgtttct attataagaa ttaacgggta
     5941 ggcttactgt ctttcttggc aaatttacaa tcaagccatt tgcagatata gtgcgcggct
     6001 acttctgcca tgattgcaaa aacaaaatca aaaagaattt tcaaacctat aacctcctcc
     6061 ccttgccaga ttaggccggg gtagcagaca gcaagactat tatagcataa ttgaaatatc
     6121 caaatgattg gatttttgct ataattttag cgaggtgatt gtgatgaaca aatatgttgg
     6181 tttgcttgat aaaatccgag taataaaaac gcaaccgtta ttggttcgct ttactttaca
     6241 aacgattcat gaatcgatta attgtgtagt tgcagatatt gaaattatcg ataaattgct
     6301 aattatggat gatggaaaat acaatattgc cgtcacaggt cacttcaata aacgaaatca
     6361 gctagttatt gctagcatgt atgttagaaa tcccgatcat tttacgcgat cgatgggcat
     6421 ataaaaaaag aagagttgca atcctaatgt aggactgcag ctctttttgt tttacataga
     6481 aacaagaaaa atgaaaaggc ctttttagga aggcgcgagc gccttctcaa ttgaaaaacc
     6541 tctctcactc caaccgccac cgccgttcga gaagaaagga agtgaacgtt gcacgttcac
     6601 taagataaag ggtcttgcaa agcaagacaa aatagaaaaa acttcctctc accaaaaggt
     6661 tcgttcctgt tcgttatctc tttattgatt tgccagaccg gcaacaagtt gccttttatc
     6721 accaaaaagc gacaaggaca agggtaaatg gcacgctgcg cgcccttgtc cttgtcgctt
     6781 ttcgttgatt cattctggcc aacaaatcaa taaagaaaga actaacgaaa ggaagtaaaa
     6841 cctttcctaa gaggaggcag aatcaatcat gcgaaagaaa tttgattatt ggggtgttcc
     6901 cttcagcgaa ttattcccaa actatcatgc accacatact gtcgaatgcg attgtggaca
     6961 aagagcgaaa tgtatcaaaa gctatcgttt gtatcaatgt ccaacatgcg gaaaaaaata
     7021 caccttgtct tatggcgatt atattttgat tgatgagaaa ggaaagaaac gctgatgaaa
     7081 acataacata aattattgac tgtaaaagaa gtcgttcaaa ttttggtgga caaagaagtc
     7141 atttctgaaa aagtagcaga agctttactt attccgcggt actggacgat agaaacccct
     7201 ttagtagagg atgcaccgac tatgctattt gaagacctag tgaagattgc aggatatgaa
     7261 ctgcattatc aagaaaacat ggaaacttat gaaaacgaat attggttaac caaattagag
     7321 aactaaaaag gagaaaaaaa tatggctcaa aattactatg acgaatttgt caaattacct
     7381 ttggataaaa tggcacaaaa aatggaggat atgacattcc tataccatga gacacgtgtt
     7441 ccaaaaaaac attataagga aagattgtcg gtagctgtgg aagaaatgat tgaatcaggt
     7501 gtcgaaatga atctcattgc gacgtactat cgaacactgg aagaactgaa aaaacaaaat
     7561 gcaaaatggt ttttccaagc cttactatgc ttggaagtag gtgtcaaacc aagcactatt
     7621 aaaccttctg aatatcaagc tttagaacta acgtatggga aatttgtcga aacgaaaaaa
     7681 gccaagactg tttcaagtga atggttagat tattttgaaa atatcaataa gtatggcgcc
     7741 tattacacca tgaaaaagga ggacaacgaa aatgaataag gtgatttatc aactaacaac
     7801 ggaagatgtg tcacactttt taacaaacaa agaacatttg aatgaagtaa tcagacggat
     7861 tcaaaaagac tttagttgta tggaagaaat gaagtacttt attcaaagag tcgaagaaca
     7921 attgtatttg gaaagtgaaa cgagtcaaac gaaggtcaca gtcagcaata attatttaga
     7981 cgtgtataaa catcgtcctg aaacggtaaa aaaaggaacg gagcttctac gactagaaac
     8041 agaaaaatac atcatgaatg tttacctaga aaggagtgaa ctggtcgaaa actttgtcag
     8101 ctgcacgatt tgcaggaaac actattactc agaatcagaa tattattcaa tttttgagag
     8161 catggaatta atggaagaag aaatggaaga gctaaacagc aaaaatgcga cgatagacga
     8221 ttattatctt gtatttacta gatttttgac gtgtggaacg gaatctatcg tgttaatggg
     8281 aatggattat gaactcgctg tacccgaagc atttttgagg gaatatgtag aagaagtaga
     8341 taacccaggc gacgactggt acacgacttt aactgatttt ttagatacct acacttacga
     8401 cgatacagaa gaaatccaaa atgaacttga ccgtaaacga atgccatatc aacgatttga
     8461 aaggaaaaaa aggcaataat catttatacc gaaaagtgag aatgtgtgaa ataagaacaa
     8521 aaaccttgaa attaagggaa tggttcttat ttcgcaccaa tcgctccaac cgcccccgcc
     8581 gcttaggtgg tgcgaaataa gcgggcaaag cccgataacc taagagaaag aaggagatcc
     8641 atatgaataa aatcattcct ctgttgttcg tactgtgtat tggcgagata cttatgcgtc
     8701 atggtgcagc tgtacattta aacttcgtag tattcttagg aatactagta ttttgtttga
     8761 ttttttggcg ctttgtgtta gcccttgtga gtctactatt caagttgatt gcagccatat
     8821 ttttcatagg gttatttcga tgaaaggaga gagtactaat gccaattgca ataggaaata
     8881 aacggttacc cgtgacatta gatgaaaaga gacaaaaaga attgcagcaa ctaaagcaga
     8941 agtacggaaa aagtgaatct aagattatgt gtattgcgtt agatctattg atcgcgcaag
     9001 aaaaagcagg atttgatata ccagcactaa aaaagtgacg tcacttttca tttgaagaaa
     9061 caaaagtgat agcacttaat tgttcctact atttagaata ttttctatgt cctttttatt
     9121 ccattttgta tcaataacta tgaaatcttt aaagtatcca gatcaatttt tttcttacct
     9181 gattctaaag attttgtttt cttaaacata ttatgatttc ctttcgacta ttttcatata
     9241 aatgttaaca tagtttatgg attcatggaa tgttaatcta atttaataga aaaggataat
     9301 taagtatata cgttttttct tatgtatgtt atactatgta tatacagaaa gaggtgtcta
     9361 ctatggaaat gttacctata aaaaaatggg gaaatagtaa tggccttaga ttgccaaagt
     9421 atattatgga atatcttgga attcatacag aagacaaagt gaagataata caagaagaag
     9481 taaacggtca gaagcggcta attatcgaag cggctaatat agaaaatgaa ttaacaattg
     9541 aacagttatt tgaaaattac actgatgaaa gaaatcatgt gacgattcaa aatttaggtg
     9601 aagcagtagg taatgaaaaa tggtgagaaa acctagacaa ggcgatattc tattattgaa
     9661 tacagcacct cgatctggac acgaacagac aggtaaaaga ccttatattg ttttaagtca
     9721 tgatattatt gcggattata gtaacgttgt tacagttgct ccaatttcta gtacaactag
     9781 aaactatccg ctatatgtaa atataaactc agaatatgaa atgaagacaa caggaaaagt
     9841 attattagat caactaacga caattgatta tgaagccaga gagtgtattt ttttggaaaa
     9901 agcgcatgat agtttagttg aggagttatt gatgaaagtt agaacagttt ttcaaaaagt
     9961 aagcaaataa aatagaaaca gcagcatgat caactattcg tgattgacca tgctgtcttt
    10021 ttttattcta tatacggttt gtctcgtaac attgtagtct tttgcaatct tagcgatcgc
    10081 aacacctttt tttagatcct caacgatgtt tttataaaca agacgacgtt ggggatcttt
    10141 tgcattggca ctgtatagct taggacgtcc tttataaaca ccgttcgctt ttgcaatctt
    10201 aatgccttgt gcctgacgtc gtttagattc ggtacgttct tgttcagcga tcatggccaa
    10261 aatttgaatg atcaaatctt taatgaattt atccaggaga ggattgccaa ttgcttcggc
    10321 catgatcggt agacttgtga tgatcagctg cacatttttc ttcttcaagt aattgactga
    10381 agcgatgatc tcgtcgtaat ttcttccaag tcgatcaata gcttccacaa taaaaatatc
    10441 ttgatctcgg acgaaatcca gtgcttcttg aaaaacaggt cgatgagtaa tcgttgcgcc
    10501 agattgtttc tcaatgaaaa ttctctctgc accgaatttt ttcatttctt caatctgccg
    10561 ttgttcattt tgatcgatgg atgagacacg tatgtatgca actctcaaaa tttttaccta
    10621 ctttctgcaa ctatttaaaa aacaccctaa tcatacacga gaaaaccaga tttttcaatg
    10681 tcgcggtcgt gtctagttag ggtatacctt atatagacaa aaactaatga tggaggaaat
    10741 agtatgagaa ttttttgtaa gtgtctactg ataacttatt ctcaaatgtt gtaaggattt
    10801 tattcatttg ttaatattaa agaacggaac tccaaaaaag tccgtactct aatttgcggt
    10861 ctctcactct tttagagtga gagtttttat tagattttca aattaattag taaagtgata
    10921 gaataaattg atcatacaaa tcataagaag aagcagggtg atagcatacc tatgctt
//
LOCUS       DANKEF010000075        10918 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000075 DANKEF010000000
VERSION     DANKEF010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.075"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..561
                     /locus_tag="PWZ48_002397"
     CDS             <1..561
                     /locus_tag="PWZ48_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HDL2262035.1"
                     /translation="PVFSIADLRKKFSIEKKYKAFRDFERRVIKEPIAQLNKYTDFKI
                     DYELIKKGHTYTNIKFLLTRTMSVPVPYKQEQRDPAYLKGAEDKTEKMNQSYLKAMNS
                     KYTKLLIKNFLISSADLCDLKLMSRLQNKVYPLYDRLSKDRGFESVVNHLSYVATHKE
                     DYTIKNTVKYLEKSITSYLPYVNLYD"
     gene            596..1363
                     /locus_tag="PWZ48_002398"
     CDS             596..1363
                     /locus_tag="PWZ48_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262036.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1555..1953
                     /locus_tag="PWZ48_002399"
     CDS             1555..1953
                     /locus_tag="PWZ48_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2262037.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
     gene            complement(2260..2634)
                     /locus_tag="PWZ48_002400"
     CDS             complement(2260..2634)
                     /locus_tag="PWZ48_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262038.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
     gene            complement(2656..3033)
                     /locus_tag="PWZ48_002401"
     CDS             complement(2656..3033)
                     /locus_tag="PWZ48_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262039.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            complement(3023..3418)
                     /locus_tag="PWZ48_002402"
     CDS             complement(3023..3418)
                     /locus_tag="PWZ48_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDL2262040.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            complement(3485..3670)
                     /locus_tag="PWZ48_002403"
     CDS             complement(3485..3670)
                     /locus_tag="PWZ48_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDL2262041.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            complement(3752..4006)
                     /locus_tag="PWZ48_002404"
     CDS             complement(3752..4006)
                     /locus_tag="PWZ48_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262042.1"
                     /translation="MNYTDEEQITLEYVFIDEEGEEIEKVLIISSKDLENALTYEPYE
                     STVTVNEFLDTYTSDDTWEIMNICNANNIQFAEKTNKSFY"
     gene            complement(4028..4273)
                     /locus_tag="PWZ48_002405"
     CDS             complement(4028..4273)
                     /locus_tag="PWZ48_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262043.1"
                     /translation="MWTLEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTS
                     LECVWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            complement(4280..4564)
                     /locus_tag="PWZ48_002406"
     CDS             complement(4280..4564)
                     /locus_tag="PWZ48_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262044.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQVYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            complement(4667..4849)
                     /locus_tag="PWZ48_002407"
     CDS             complement(4667..4849)
                     /locus_tag="PWZ48_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HDL2262045.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKATGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            complement(5055..5594)
                     /locus_tag="PWZ48_002408"
     CDS             complement(5055..5594)
                     /locus_tag="PWZ48_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262046.1"
                     /translation="MYNISVNKLINFQKERFFMGWYGDYSSANEVIEEKKGYAKNGLE
                     IVDTKVTKIYGIILFKDAEGTYDIDSFIFRDEMYKPIYFLNDSPAYFKKIPKKWLKFF
                     VGDQEKMVKRQLDRIKEESNKPKLDDLLVAGKTYSIWDGSYVAVYQYKLKRSFIFRLQ
                     DGSLTRFTRLSAGDVKEVA"
     gene            complement(5707..5973)
                     /locus_tag="PWZ48_002409"
     CDS             complement(5707..5973)
                     /locus_tag="PWZ48_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2262047.1"
                     /translation="MTYQVRYEREAQKALKKMDKFQAKVILNWIEKNLVGTDDPRKHG
                     KGLTANKSGYWRYRVGAYRIIADISEHEVTILILSIGHRSEVYK"
     gene            complement(5975..6196)
                     /locus_tag="PWZ48_002410"
     CDS             complement(5975..6196)
                     /locus_tag="PWZ48_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDL2262048.1"
                     /translation="MPVISIRVSKEEKDFLEKVAEFNGDNISEFMRSNSLQTAETLVD
                     FATYKQLMKEHEENDQSISHEDMLKELGL"
     gene            6422..7240
                     /locus_tag="PWZ48_002411"
     CDS             6422..7240
                     /locus_tag="PWZ48_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2262049.1"
                     /translation="MEKKLSIITGNIEKGGTGKTTTIYNLGGYAVENLGMKVLYIDED
                     KSQNLSKRFPDAFSKAKRENTINHLYLHGSAEPLAINENMDILVAGEDLKQVEMQMRD
                     MPNNRTLLFRWIVVNYEQLKEKYDLILIDTHNDAGLLAQNAWAVSDLVVGISDPSKDG
                     FEALLKLGKDIDALAKQLIDIKTGETLMETKYATIGSRIKHNTNSSREFLELVPTLSN
                     YLGYVQEKELLNATSLEGKNIAEFAKDKNIYRKNKDFFESTFTLYNKIIDTAQE"
     gene            7244..7540
                     /locus_tag="PWZ48_002412"
     CDS             7244..7540
                     /locus_tag="PWZ48_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262050.1"
                     /translation="MIEVKENRFKKFAEAEVEEVPEVVEAVEEPKQTPKETTSSLVAK
                     LKSDSSEKKKNYQFTLQPSQRKKLSKISEKLGIRSDSALLGEMIDNLYDEVFGE"
     gene            complement(7722..8075)
                     /locus_tag="PWZ48_002413"
     CDS             complement(7722..8075)
                     /locus_tag="PWZ48_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262051.1"
                     /translation="MNKGKSERYVVIINREKVSELPLAEFLESEYETLGVKYSQTIKK
                     ALNLYRRVKNNEVTIIEAGNDRNDSKVAEVKKKPTDHNPIQQEHPSTVTSDHSAKKEK
                     PMLKGFNAPSNKDLL"
     gene            complement(8095..9150)
                     /locus_tag="PWZ48_002414"
     CDS             complement(8095..9150)
                     /locus_tag="PWZ48_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HDL2262052.1"
                     /translation="MKEPLLFSVDLGNKSVKEMSTHVSPLSLPSRILNKNIINSGRFS
                     NIGSSLKRSLNVSEYSLVNTTESYYFGKDIHELGKDDYVIESLGFGISRYKQETYKNI
                     LSFSLANLARLEDQSDDVLTVNLVLGLPSSDYTETIVKYVMQQATRQHSVEVDGKAYN
                     INVDTVWVLPQPIGTLYNLLLADDGSVQDETLISQKIGFVDIGGGTKLFDVVQNFEID
                     ENNREQKDTGIFTLYNRIVNMLDLPISPNAFQLEKIIREGKEKGVYLFKPSGNESFDI
                     SEVVEREIKTYTNMVVAEVLATFKNFTTLDKVVFTGGGASIIDKQLVKDKIPHTLFVE
                     DSEFSNVKGFYKFGLTQ"
     gene            9366..9764
                     /locus_tag="PWZ48_002415"
     CDS             9366..9764
                     /locus_tag="PWZ48_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262053.1"
                     /translation="MRLRRNKNYTTIILEDFTDQELLIVGKTKADFIEKGNASIVKAS
                     KVAVEKYFDSLEESAKEIFNGEKAGSYELDGIRFSLIPTTENIIPKKLYYIFSSEKHT
                     SMKPCIFDWQWLIDAVLDVKCSKYNITLGC"
     gene            9767..9940
                     /locus_tag="PWZ48_002416"
     CDS             9767..9940
                     /locus_tag="PWZ48_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262054.1"
                     /translation="MSMLVRKLPLSVREFLFYLVCLIASGFKLIEHIFIFAYVLAMIA
                     FLSGGWLIIYLLS"
BASE COUNT         3635 a         1679 c         1859 g         3745 t
ORIGIN      
        1 cctgttttct ccattgcaga tttacgaaag aaatttagta ttgagaaaaa atataaagct
       61 tttcgagact ttgaaagacg tgtaattaaa gaaccgattg cccaactgaa taaatacact
      121 gattttaaaa ttgactatga gcttatcaag aagggtcata cttatacaaa cataaagttc
      181 ttgcttacac gtaccatgtc agttccggta ccttataagc aagaacaaag agaccctgct
      241 tatttaaaag gcgcagaaga taaaacagag aaaatgaatc aatcgtactt gaaagctatg
      301 aatagcaaat acactaaatt actaatcaaa aactttttga tcagttctgc cgacttatgc
      361 gatcttaaac ttatgagccg acttcagaat aaagtttatc cgctttatga tcgtttatcc
      421 aaagatagag gatttgaatc agttgtcaat cacctatctt acgttgctac tcataaggag
      481 gattacacaa taaaaaatac tgttaaatat ctcgaaaaat caattacgag ttatttgcct
      541 tatgtgaacc tttatgatta acaactgaaa aaatgcgtga aggaaggaaa aacttttgaa
      601 aaaagatgaa ctgacaaagt acaatgagga gttcttagca acaattgaca tgaaaatgga
      661 tttaaagtcg tatgaacttt atcaattgct gttcagttat atctcaccca caaagctaaa
      721 gaaaacaggc gtagtagatt tacctgttaa agcgattatt aattacatgg atgttgatca
      781 aaatcttgaa acggaactaa aaaccatagt taacactttg caaagacaag cagtttatga
      841 aatttcgaat ctagacaaaa aatataagac gtatactact gtatctgcta ttgatcggat
      901 tcattgggat ttagaagagc atagtgtatc tttcagtttt tcagaagatg taattccctt
      961 aattgtctca atcaaaaaga agtatccgtt ctttgttttt gaagacttag catcttttca
     1021 aaaaaaatat agcatcgtgt tatacaaata tttggcgatg tattataaaa aatatcttat
     1081 cgccaaaaat aaaggagatt ttgagaaagc tgaaatatat aagaatcccc tgattgaaat
     1141 agttaaaatt cgtgagatta cgaaaacaga aaatgagtat aaaacaatat cgaattttga
     1201 tagacgtgtt ttaagggaat cagttgagga aattaatgaa aaatcaatgc tgaatattga
     1261 ttttgctaaa gttagaaaag gcagaaatta caattttgct gtccagttta atatttctag
     1321 tgagtttgat gaaaaaactt atagagaaaa aaaattaggt taatgcaaaa tatatcttac
     1381 tcgtttgaag atgcttcaga cgtatgctaa gatatatagt gccttgggag caaggtatta
     1441 atctccttta gaggttaatt agcttgtgat cgcctatcaa tttttttggt aggcgattct
     1501 tttttttgaa acaaaaaagc aaaaattatt ttttaattag agaggtgcaa tgcgatgaaa
     1561 tacttttttt gtgcagtatt cgaatggaaa ggaaatgaat atgcagttaa attcccagat
     1621 tttccagact tatatacaca tggtgaaact ttagaagatg cttttgagat ggcgcaagaa
     1681 gtattggaag gatacttggt gcttttagag ttgcaagaaa aagaaattcc atcgcctacg
     1741 cctgttcaag agttaaatgc aagaatcaag cgatctgaga atgttaattt atttgtcgta
     1801 aaagctaata cgaaagttgc taaagctcga aacaagcaaa attatgatcg aaaatcagtg
     1861 actgtggaac actatttaaa tgttttagcg gctgaagaga atattaatct gtcgaaagct
     1921 ttgaatgatt tgttgagaga aaagttgatt tgatgtctct cagatgatca tattaagcaa
     1981 aaacagcttg ttttaggacg tatggattta agaaagacaa attatcaaag aatatattaa
     2041 aagctctcag aagccttctg agagctttta aattgctatg tattgtactg catctgctca
     2101 aattgatttt cttttcatgc ttaccatgta tctaattttc catcaagtaa tttttagata
     2161 tgtattgagt aataaatagc aaatttgctt gatataagct cgaattaggt gctttgtaat
     2221 gtgttaggat aaaattatac aagaaaatgt caaaagctct cagaacgctt ctgagagctt
     2281 tttatcggta ggcttgttta acgtgatctt gaaggatacc cttttgatgt ttctctcgat
     2341 ccctgcatcg ctaattacta agtgttcttc aagtgtgatg taggtatata aattaagcat
     2401 caaaatggtg tagataagca aaaaactccc taaaactaag tgttgattat aaaaatctga
     2461 cttataaaaa agaactgaga atgttagtgt tgattcccaa aaaagaaaca aaattttgta
     2521 tttccaaaga tagtggatca agcgctgtgc tttacccatc tcaaataatt tataataacc
     2581 tgctaattga tcagcaagcg tatcgttaac ggtttctgtt tgtgtttgtt tcatctttct
     2641 cttctcctgt ttcaattaat ctaaaatttc agattgagca agcaactcat tgtggtagtc
     2701 ggtttgaaat tcaaataaca attttgcctt cttaacgagg tttaaagcgt tcgttagtga
     2761 ttcacttaat gcttcaacat ctttaactga ttttgcatgt ttcatttttt cagtgtgcgt
     2821 atagcagaat tcaggtattg agttaggggt tgcctgttca ataaacatct caattcgcaa
     2881 gttattatcc tcaaaaataa ttagatcatt ggcaaagtgt ttggtactaa cgctaatgcc
     2941 agttgtatct tttaatagat tattgattaa cgttcgagag aaatagttta agtgtgtagc
     3001 tttcataggt tcggtctcct ttttactttt caagcaactc ttcttccagt gctttttgaa
     3061 gtagttgaga gaagtttatg tttttctcct tccctaaaag aactaaccat tccggaacac
     3121 taacattttt tctaattgtt tttgtattta agcttcttcg gaaaagttta atgtttagtt
     3181 ggatgattac taccacttgg ttggctttca aagttaattc ttccactgct gatggtttag
     3241 gaaattctgc atctggtaca ctatatagcg ctaatcctaa agcatcctta gcgtttaaat
     3301 atgcttcttc aagactattt ccaaaggtag atatttccgg taagtcaggg aaaacgatgt
     3361 tataagcttc tccttctttc tcgaagattg ccggataaaa aatatttttt gtcttcattt
     3421 cctcgctcct tttctagtgt aagagaggat agtatgccta ataccaaata ggaaggcaag
     3481 gctgttaaag ccctgcttgc ttccagatat tagcttctgt tcctagtggt acatcaccat
     3541 tgtggatcgg aacaacgacc gttcttttag tcttggaatt ataaaatttt gcatgactgc
     3601 cagtttgtcc agtctttttg aagccgttag atttgagaat ttttacaatt tctcttgctg
     3661 tcttaggcat agctaccctc cctttacaaa atctattata cacattgttg tgtatggaat
     3721 caatagaaaa agaggatgtt ttattgcaaa actaataaaa tgatttattg gttttttcag
     3781 caaattgaat gttattagcg ttgcaaatat tcatgatttc ccaagtatca tcagaagtat
     3841 aggtatctaa gaattcattg acagttacgg ttgattcgta tggttcataa gtcaaagcgt
     3901 tctccaagtc ttttgatgaa ataatcaaga ctttttcaat ttcctcccct tcttcatcta
     3961 tgaaaacata ttctaaagtg atttgttctt catcggtata attcatattg tgttctcctt
     4021 tcttgtacta agaaaaatgg atatttccaa ttgcatccca ctcggcacca ttgtggtata
     4081 ggacgtgacc gagcgttacg cctttgaaat gttcttctaa atacatccaa acgcattcaa
     4141 ggctagtacc tgctttgaga ttgtagaagt ctgcatccaa taccttttca gaagttatcg
     4201 gatcaatgcg agttggtact tcaataaatt cagtccaaat ttctgcaaaa gtcttacctt
     4261 ccaaagtcca cactccctat caattaattc caaaagcaaa tagagaaatc aagcgaccgt
     4321 caatagtcac ttcaacaact gaaaatgccg ttgaaacagt tttaacatct acactttcca
     4381 agttcccgat gaaggaatct ttgtgcttta gaatagcttt ctttagctgt ctgttataca
     4441 cttgccgact gttaaacttt ttgaattctc gatccttgat cagctgttta acgatccctg
     4501 cattaattgt ataactttct gcaatagcaa cgtgagtagg ttgctgtaaa gtagctgtta
     4561 gcatcttgtc actccttcct aaaagttaat tagcttgttt acaaatacat tatacacatc
     4621 ggtgtgtata atgtaaagag aaaaaggtgc taatggatca gctgttctaa acaaaagaaa
     4681 acgataaagt aacaaataaa gtcagcaaag ttaaaaagga tgaaacggct agtccggttg
     4741 ctttgcttgt gtgattttcg gctttcttat agatcaaata ggaaattatc agtgatattc
     4801 cagtaaagaa taatgtacta gctgtcacta aaagaaatag gttaatcata cgatacactc
     4861 cttctgtaat attggtatca gatacgttta aatgcgtttc tgagctgttt tggatcagat
     4921 ggtgatgttt ttagccctac cctaaaaaca aagcccagaa aagaatttaa aagcgttcgt
     4981 acctaatacg tttatattca tatacatata cataaacata aacgtgtata cgtttatgtt
     5041 tatgtattta agttttaggc tacttcctta acatctcctg ctgatagtcg agtgaaccga
     5101 gtgagtgatc catcttgtag tcgaaaaata aagctacgtt tgagtttata ctgatagaca
     5161 gcgacatacg atccatccca gattgagtaa gttttacctg caactaataa gtcatcaagt
     5221 tttggtttat tgctttcttc tttaattcta tctagttgtc gcttaaccat tttttcttga
     5281 tcaccgacaa agaatttcaa ccattttttc ggaattttct taaagtaagc aggactgtca
     5341 tttaagaagt agattggttt gtacatttca tctcgaaaga tgaagctgtc aatatcgtag
     5401 gttccttctg catctttgaa aaggataatt ccatagatct tcgttacttt ggtatctacg
     5461 atttccaaac catttttggc gtaccctttc ttttcttcga tcacttcatt tgctgatgaa
     5521 taatcaccat accatcccat aaaaaatcgc tccttttgaa agttaattag cttgtttaca
     5581 cttatattat acacaccggt gtgtataata tcaagaggat attgttcttt tttaggtaaa
     5641 gtgggatcgt aaacagtgat tttgttgggt taacaaaaaa acagacaagc cttatcagct
     5701 tgtctgttac ttataaactt cacttctgtg accaatagac aatataagaa ttgtcacttc
     5761 atgctcgcta atatcagcta ttatacgata tgcacctacc ctatatcgcc agtagcctga
     5821 tttattagct gttagacctt taccatgctt tcttggatca tcagtgccta caagattttt
     5881 ctcgatccag tttaggataa cctttgcctg aaacttatcc atttttttta gcgctttttg
     5941 agcttcacgt tcatatctta cttggtaagt catcttataa gcctaactct tttagcatat
     6001 cttcgtggga aatactttga tcgttttcct catgctcttt cattagttgc ttgtaggtgg
     6061 caaagtctac taacgtttct gctgtttgca gggaattact acgcataaat tcagaaatgt
     6121 tatctccgtt gaattcagca actttttcaa gaaaatcttt ttcttcttta gatacacgaa
     6181 tacttattac tggcatagtt tatcaactcc ttttcaatta cattgtacta cgattgtatg
     6241 acaaatgcaa tagttatata tttgaatata tacataaaca taaacatata catatatatg
     6301 tttatgttgt agaatataaa agcaaatata aatataaaca cgtttatgta tataaacata
     6361 tacataaacg tgtttatagt gtataatgga gttacaaaag gttaataagg gaggtttaat
     6421 catggaaaag aagttgtcaa tcattactgg aaacattgaa aaaggtggaa caggtaaaac
     6481 aacaacaatc tacaatctag gtggatatgc tgtcgaaaat ttaggtatga aggtgctgta
     6541 tattgatgaa gataagtcgc aaaacctatc aaaacgattt cctgatgctt tcagtaaagc
     6601 gaaaagagag aatacaatca atcatttata tcttcatgga tcagctgaac ctttagctat
     6661 aaatgagaac atggatattt tagtagcagg tgaagattta aaacaagttg agatgcaaat
     6721 gcgtgatatg cctaacaaca gaaccctact gtttagatgg atcgttgtta actatgaaca
     6781 gcttaaagag aagtatgatc taattctgat tgatactcat aatgatgcag gtctactagc
     6841 tcaaaatgct tgggctgtat ctgatcttgt cgtaggcatt tctgatccat ctaaagatgg
     6901 ttttgaagct ttgctgaagc ttggtaaaga tattgatgca ctagcaaaac agttgattga
     6961 tattaagact ggtgaaacac tgatggaaac taaatacgct acgatcggta gcaggatcaa
     7021 acataatact aattcaagtc gtgagttcct agaattggta ccaacgttat ctaattatct
     7081 tggatatgta caagagaagg aattgcttaa tgcaactagc ttagaaggta agaatattgc
     7141 tgagttcgct aaggataaga atatctatcg taagaataag gatttctttg aaagcacgtt
     7201 tacgctatac aacaaaataa ttgatacggc acaagaataa ggagtgattg aagtgaaaga
     7261 aaatagattt aaaaaattcg ctgaagctga agtagaagaa gttcctgaag tggttgaagc
     7321 agttgaagaa cctaaacaaa cacctaaaga aacaacaagt tctttggtag ctaaactaaa
     7381 aagtgatagt tctgaaaaga agaaaaatta tcaattcacg cttcaaccat cacaaagaaa
     7441 gaaattatct aaaatcagcg aaaagttagg aattcgatca gattcagctt tacttggtga
     7501 aatgatagat aacttgtatg atgaagtttt tggtgagtga tcagtttaaa gctgatcact
     7561 ttttttattg gaataagcgg ttaggtatag cttaactaaa tttcgtatac gtgtttatgg
     7621 atatatacgt atacatgttt atatacatgt atacgtatat gtttatgttt gtatttgtac
     7681 ataataaaaa ggctaggtat tgtaacctcg cctttttatt tttaaagtaa gtctttgtta
     7741 cttggtgcat tgaacccttt tagcattggc ttttctttct ttgctgaatg atcggatgta
     7801 accgttgaag gatgctcttg ttgaataggg ttatgatcag ttggtttctt ctttacttct
     7861 gctactttgc tatcgtttct atcgttgcct gcttcaataa ttgtaacttc attgttcttt
     7921 acacgccgat ataggttcaa agcttttttg atcgtctgag agtatttaac gcctagtgtt
     7981 tcatattcgg attcaagaaa ctctgctaaa ggtaactcgc taaccttttc tctattgatt
     8041 ataactacat aacgctctga ttttccctta ttcatgttga tctccttcgc tgttttattg
     8101 agttaaacca aacttgtaaa agccttttac attagaaaat tcactgtctt caacaaacaa
     8161 ggtatgcgga attttatctt ttacaagttg tttgtcgata atagaagctc caccaccagt
     8221 aaagacaact ttatctaagg ttgtgaagtt tttgaatgta gctaaaactt ctgcaacaac
     8281 catattggtg tatgttttta tttctctttc aactacttct gaaatatcaa aactttcatt
     8341 tccgcttggc ttaaatagat atacgccttt ttctttgcct tctcggataa tcttttcaag
     8401 ttggaaagca ttaggactga tcggaaggtc aagcatattt acgatacgat tatataaagt
     8461 aaaaatgccg gtatcctttt gttcacgatt gttttcgtca atttcaaaat tttggacaac
     8521 atcaaaaagt ttcgtgccac caccaatatc aacaaaaccg atcttctgac tgatcaatgt
     8581 ttcatcttgt acactaccat catcagcaag taggaggtta tatagtgttc caatcggctg
     8641 tggcagtacc caaacggtat ctacgtttat attataagcc ttaccgtcaa cttcaactga
     8701 atgttgtcgt gtggcttgtt gcatcacgta tttgacgatt gtttcggtat agtcggatga
     8761 aggcagacct aaaactagat taacggttaa aacgtcatca gattgatctt ctaaacgtgc
     8821 taggttagct aaagagaaag ataggatatt tttgtaggtt tcctgcttgt atctgctgat
     8881 cccaaaacct agtgattcta tcacgtagtc atcttttcct agctcgtgta tatccttccc
     8941 aaaataatag ctttcagtag tgtttactag gctatattca gaaacgttta gtgatcgttt
     9001 taatgaggaa cctatattag aaaaacgtcc tgaattaatg atatttttgt tcaaaatacg
     9061 agagggaagt gagagtggag aaacgtgtgt actcatttct tttactgatt tattccctaa
     9121 gtcaacggaa aacaataatg gttctttcat ggtatgacct ctttctgttt gtgtttatgg
     9181 tatttcggta cgaaaatagt ttacacctat ttactattag ttgcaactgt ttataagaaa
     9241 aaaatgcaat cataaaagtc taattaatta taataaagtg taaataaaaa ggtgtaaata
     9301 aatgcttgca aatttatctt attatgatat tataaaatca taaattatat taaggagtga
     9361 tcctgatgcg attgagaaga aataagaatt acactaccat tattttagaa gattttactg
     9421 atcaagaatt acttattgtc ggtaaaacaa aagcagattt tatcgaaaag ggaaatgctt
     9481 caatagtaaa agcttcaaag gttgctgtcg aaaaatattt tgattcttta gaagaaagtg
     9541 ctaaggaaat ttttaatgga gagaaagctg gtagctatga gttagacggt attagattta
     9601 gcttgatccc aacaactgaa aatattattc cgaagaaatt atattatatc ttttcatctg
     9661 aaaaacatac ttcaatgaag ccttgtattt ttgattggca atggttgatt gatgctgtat
     9721 tggatgtaaa atgttcaaaa tacaatatta ctttggggtg ttaattatga gtatgctagt
     9781 tagaaaatta ccattatctg taagagaatt tctattttac ttggtctgct taatcgcatc
     9841 agggtttaag ctgatcgaac atatttttat ctttgcttat gtattagcaa tgatcgcatt
     9901 tttatctggc ggttggttaa ttatttattt gttaagctaa gttttagatc aaaaataatt
     9961 agtaaattaa acgataaaat aaccgttaat aaatgtaatg aaaggacaac gcctaacaat
    10021 cagcttgtaa taagattata aaataacata atatcaaaaa tagtgctttt taaaggacaa
    10081 attaagtgta tcaacataaa cgtgtatatg tatatgttta tgttgatact ttttttgtct
    10141 taatttttta tttttaaaat tctatttact aatgctgtat agatagtgat cagaaaaaac
    10201 aaaatagcga gattaagcta tttattagtt catatattat attcagatta atatgatcca
    10261 gtattaatct gaaggcaaaa ccaatatatt tctacggact cttgtaaaac tatttaaatt
    10321 ttcctgctaa taaaataacg aaaatagaat atttagcttt tttataccat tttacgataa
    10381 catgatttca tttgtaatat taaaacattt aaatttttaa ttattctttc aaattagatc
    10441 aattaatatc cttataaaag agcataataa atggttttta catcgttatt tattgatatt
    10501 aatctcatcc aaaaaaaatg aaaacgcata cttttatggg tgattgaatg tgattttaat
    10561 acatatacca attaaaagaa aactttttag atttttactt acaaatttat aaaaaaacaa
    10621 gataattgat actttgattt tgcgaacatt ttatcttgcc gcctaattca gctttaatat
    10681 catgattata tcagtaacaa atcgcttttt tagctaattt aagcatcaaa taattattct
    10741 taatcaggga aaaaataact aataaatgga ttttgggaat aggtaaaaac ttgatcggtt
    10801 tctggctaaa aataacgtga aaaaggaaat ataaaataac atggagcgtg ggaggaataa
    10861 agaaatttta cacgaaaggt gcgcagggat gtaaagaaac ttaacacgaa acaaaagt
//
LOCUS       DANKEF010000076        10543 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000076 DANKEF010000000
VERSION     DANKEF010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10543
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.076"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            104..1147
                     /locus_tag="PWZ48_002417"
     CDS             104..1147
                     /locus_tag="PWZ48_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2262055.1"
                     /translation="MKKFLVGILFTMGLVLLSPSVSAESSISMYRMYNPNSGEHLYTR
                     SVGERDNLKRYGWHYEGVAWQAPTSGTPVYRLYNRYNGEHFYTVDAGERDKISHTGWT
                     YEGVAFYSYNGANRVPVTRLYNKYVNWHHYTLDQNEKNVISKQGWKIEGTGWYAMPNS
                     VPNPTPAPSTEKVLNVPVVWQGNTMLCEGASLLEGLHYKGVATNQSLTSFVNYMPKAN
                     DNNPYHGYSGEWRHNVNGTYQGMMADPVVKWAKNVGGNAVNITGSGTNGIKNEIRNGN
                     PVVTWITYNYATPEFKQMPWGRAVWNGHVVLVDGFRNGAYHVVDPVFGAKWINASTYE
                     RAFNVTGMAVAVR"
     gene            1320..1544
                     /locus_tag="PWZ48_002418"
     CDS             1320..1544
                     /locus_tag="PWZ48_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262056.1"
                     /translation="MNQRRRKRLEEISERLSELKDELEVIQEEEYKENIPKNLQGSER
                     YEKAEESVDQLEEAVDGLSDILELIEDIAS"
     gene            1547..1804
                     /locus_tag="PWZ48_002419"
     CDS             1547..1804
                     /locus_tag="PWZ48_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262057.1"
                     /translation="MINEDFRSENKMIDISLIEKLLFSNKYTAKELATRWQISESAIK
                     AYRQNKGSVGWRDWKGISIGKAEDIMKREGLRSYTTSEKDK"
     gene            1906..2259
                     /locus_tag="PWZ48_002420"
     CDS             1906..2259
                     /locus_tag="PWZ48_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262058.1"
                     /translation="MISENLSYENNKHLVKENKKVEDYKNMYIEMKKSPLCIQYKNDN
                     WKQVVEFVKPHSIRRVRRGKKKSELVNSGRAVIRVITKDYPVEYGDYILRLSYHAFVV
                     LKEEHFNEFFNLKSK"
     gene            2312..3106
                     /locus_tag="PWZ48_002421"
     CDS             2312..3106
                     /locus_tag="PWZ48_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262059.1"
                     /translation="MVAKKNTLKEASEKYQDMKRRGLIPKDKKGIWREFVKEVSSDRS
                     GGDFVGRYTAKGYSKRLRITVEYGTNESATELKQRYLDTRREASKKYNDFKSRGLIPK
                     KKKGIWREFFKEEDSNRNKGDFIGRYTAKGYTKNLGIKVEYGTGESSWELRNRYLNTL
                     LEKYKAELLELFDVNDFIEMAEKYGHEEGFMINDIMVIDNFTDLLALAKKARDLRETQ
                     MYLFRNKNGRNRRTESSEIEDYYDAFTHDLGAFVDEISGVMNKYRV"
     gene            3135..4253
                     /locus_tag="PWZ48_002422"
     CDS             3135..4253
                     /locus_tag="PWZ48_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262060.1"
                     /translation="MGNNSIYTPEMQNKIKRLVGLGWMTRGKLDSFSTIKYLLMDIYR
                     QNKQTGIHFLGYDKPEDAPFFVPSSAYRASTSGSMYNLDLFEEGAKMIEYDFNEAYSR
                     IMRNYPLPSNTYLKAKYNTDQAILRAINRPKVRPYENLRTYMFFLVNFEGTAKEGTYA
                     NPKSQSMFADYGENPKTANEPIWLSEIELTLILDYYDIKEMEVLDSHTFLTKKGLLKE
                     YFERIEPLKTDPDTLKLYKNLRTKVFGAIGQKSLREGETSTFDKYSMYNRAYSSMVAG
                     VFRDRMVRYEQKYVHSAYGLLKIRTDGLYFRSEVPEFEELYKLGVVKKKVHTISKEEV
                     EKALEFKNKGFENKGSFGKSKDEVKGVVIDDDFEFDFD"
     gene            4286..4606
                     /locus_tag="PWZ48_002423"
     CDS             4286..4606
                     /locus_tag="PWZ48_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262061.1"
                     /translation="MKKKIVEGNVGVIILKDSTDYAKSMALKDEKVARMDKDLVEFLM
                     DDTERLKGDQFLSLMKDKGLNTYGLFDYKKLAIEWVKEGEIFFIEEWEGTENLVLHQY
                     LLKA"
     gene            4861..5550
                     /locus_tag="PWZ48_002424"
     CDS             4861..5550
                     /locus_tag="PWZ48_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HDL2262062.1"
                     /translation="MRTHYIYLEDKIVDEIPAKSREQARKLFIEKYKEINGDSEVPKV
                     VVTVRKRQTKKRSTFHVSPDIYNLIERHRSEDNISISQYIATVIDELISGKYDYAFDF
                     EGEKDYKSVAVFQSDFEKLKNYAGRRNVNFARLLEEFYSEELGVGNEYKRETYAKLSH
                     KLSQALKEVTEVKAELDAKLEEVKKYQTERKNNMKNQVEFDEQKEFEKARKRYNKIMN
                     ELSYEHLTVHE"
     gene            5551..5754
                     /locus_tag="PWZ48_002425"
     CDS             5551..5754
                     /locus_tag="PWZ48_002425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262063.1"
                     /translation="MSEGTEKYTIADMVNEVETLLASFFENGHVNDEWRKEEPKRVNA
                     YIARLKRFIDKYKENSDKFSKDK"
     gene            5774..7402
                     /locus_tag="PWZ48_002426"
     CDS             5774..7402
                     /locus_tag="PWZ48_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262064.1"
                     /translation="MPEIEGIFSPKIGKKQLDFSSFPSVNRDYNLGDEVQILNIDFKE
                     LDFAVAVSNKPKALERVKKIYQENDQNIKNMIKKYRRDNSKVEDVLFNGTAKHDLNVY
                     MALGVALYCNSINQTDILVNIVRNSYQKILKISSETNPYYAMKYLGDNLLNDKKLMHN
                     TEELLSNLAAINFLFKTGSEDYFIDNVLKGAYGTAFYPLYNMKQHKAEQKDNKLEIKK
                     MKELLGIGFVKPNSKVTIASLATTNNDGKIVNGFDDSDIDILSKSYFSLYGDFADLMG
                     IDSYLVNNFEIKGSELNDIVYFVLISLYAIDQNTEYRQIDYLLVFYSLLYQFVLTRLF
                     KNAKEELILNSDYEIAKQIASIRKENKSEVIKLRKELSIASKENGRLESLVKSAKKEE
                     ELLKQEIKELKGIRKELEQLRFENKVLKQLQEQEHRQSLNGKTEEPSLSEMIAYLKHK
                     NILLVGGHVNFQNKLTELLPNIKMFDVSENNGKILKNLNTCDAIFLYTDYLNHGLYYR
                     VMSEISNKDKIFYLDNHVNMNLTIKRIYDLLSLI"
     gene            7453..7824
                     /locus_tag="PWZ48_002427"
     CDS             7453..7824
                     /locus_tag="PWZ48_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262065.1"
                     /translation="MRELTLSSKELEKKTALLNEIFSADCIIQSLKESSFSKLTDQEK
                     ISYMLSLDNLGTKYDESASSDKLLIELVSSLSSFGKMKRFNVILEIFAWLIPSFIWFF
                     YIFIVESELIAKVSLGLLIPI"
     gene            7909..8109
                     /locus_tag="PWZ48_002428"
     CDS             7909..8109
                     /locus_tag="PWZ48_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262066.1"
                     /translation="MLKRIAWDKDNKTDIELKEYKYNYFSRCIDRKLLFKKEGLEFSF
                     DEDEFEKLVMDWRKNLLDTVQF"
     gene            8243..9469
                     /locus_tag="PWZ48_002429"
     CDS             8243..9469
                     /locus_tag="PWZ48_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262067.1"
                     /translation="MIFYMFININEVLKLTTITMTKKIEVYVDGSQEERDSFFDFFWK
                     NSREYVKALNLVYKQLMFEELIKDAIKEMDQGYAEREESIQNRIEKLAYTDPIDQKKL
                     DKAKAELSKLRNEKNKEAREILTQAIGLKSQTRTRDVAKKLDFDFGDFVDSANMKASK
                     DFGNDFIGIVTGQRTPRIYKNRNKYGLLPFRGRDIKIHKDDKFYIYLPKGFRFKVNLG
                     SKPRKARDVASVLQHIMDGDYKLNQSNLIFDGRKLFFNLTISFEKSYNEMEFKKGNEL
                     IVNFADLCSGECLINDKSVMSFGDSQYIRNFKSKILAMKSKEQSRGVFSKGGHGRKRK
                     LDQDRWEAIKKRESNFVKTYNNQCSAKIVKTAIRNKAQIIRIIEPDKAYGEWAYYQLK
                     EQLVNKGKRYGIEVRS"
     gene            10066..10461
                     /locus_tag="PWZ48_002430"
                     /pseudo
     CDS             10066..10461
                     /locus_tag="PWZ48_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334017.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
BASE COUNT         4103 a         1281 c         2100 g         3059 t
ORIGIN      
        1 caacaacgat tatgatctta aaacaatcgt ttatgtaatt ggcaatctta cgaataattt
       61 tcatgcaata atgacaataa aaaatgaaaa ggggtaaaat caaatgaaaa aatttttagt
      121 aggaattcta ttcacaatgg ggctagtatt attatcacct tcagtttcag cagaaagctc
      181 tatttccatg tatcgtatgt ataatcccaa tagcggagag catctataca ctagaagcgt
      241 tggagaaaga gacaatctga aacgttatgg atggcattac gaaggtgtcg catggcaagc
      301 tccaacaagt ggtactcctg tttatcgttt gtataatcgg tataacggtg aacattttta
      361 cacagttgat gccggagaaa gagataagat ttcacacaca ggatggacgt acgaaggtgt
      421 tgcgttctat tcctacaacg gagcaaatag agttcctgta acacgtttat ataacaaata
      481 cgtgaattgg catcactaca cgttagatca aaatgaaaag aacgtaatta gtaaacaagg
      541 ttggaagatt gaaggtacag gctggtatgc aatgccaaac agcgtaccaa atcctacacc
      601 tgctccaagc actgaaaaag tgttgaacgt tcctgttgta tggcaaggaa atacaatgtt
      661 atgcgaagga gcttctttat tagaaggttt acactacaaa ggtgtagcca caaatcaaag
      721 cttaaccagt tttgtaaact atatgccaaa agctaatgac aataatcctt atcatggtta
      781 ctcaggagag tggcgtcata acgttaatgg aacgtatcaa ggcatgatgg ctgacccagt
      841 agttaaatgg gcaaaaaacg ttggcggtaa tgccgtcaat atcactggtt caggaacgaa
      901 tggaatcaag aatgaaattc gcaatggtaa tccggttgta acttggatta cttataacta
      961 tgctacgcca gagttcaaac aaatgccttg gggtcgtgca gtatggaatg gacatgtagt
     1021 tctcgtggat ggcttcagaa atggtgcata ccatgttgtt gaccctgtat ttggcgcaaa
     1081 atggatcaat gcaagcacgt atgagagagc atttaatgtg actggaatgg cggttgctgt
     1141 acgttaattg atagaattat caactaaatg aaataagagc ttaattactg aaatggtaaa
     1201 taggctctta tttttttaga ggaaattttt gtaatacgaa tctataacaa aaaatattga
     1261 atgtagtaca caaaagtggt atattaaaat cgtagagatt atgcgaagag gtgattttga
     1321 tgaaccaaag aagaagaaaa aggttagaag aaataagcga gcgactttcc gagctaaagg
     1381 atgagttaga agtaattcaa gaagaagagt acaaagaaaa tatacctaaa aatctacagg
     1441 gatcagaaag atatgaaaaa gcagaagaat ccgttgatca acttgaagaa gctgttgatg
     1501 gtttatccga catattagaa ttgattgaag atatagctag ctagagatga ttaatgaaga
     1561 tttcaggagt gagaataaaa tgattgatat ttcactgata gagaagttac ttttctcaaa
     1621 taagtacacc gcaaaagagc tggctacaag atggcaaata tccgaatcag ctattaaagc
     1681 ctatcgtcaa aataagggaa gcgtaggatg gcgtgattgg aaagggatca gcattggaaa
     1741 agcagaagac ataatgaaac gtgaaggact tcgatcctat accacttctg aaaaagacaa
     1801 ataacttaat gtttgaaaag ataaaatatc tgtcttaaag ctatgtcaaa ttcaaatggt
     1861 gaatgtttgc taaatataat gcaaaaaaag gaagggaaat attaaatgat aagcgaaaat
     1921 ttgtcttatg agaataataa acatctagtt aaagaaaata agaaagtaga agattataag
     1981 aacatgtata tagaaatgaa aaaatcacca ctgtgtattc aatataaaaa tgataattgg
     2041 aagcaagtag tcgaatttgt aaaacctcat agtatacgaa gagtacgaag ggggaagaaa
     2101 aaaagtgaac ttgtaaatag cggaagagcg gtaattagag ttataaccaa agattatcca
     2161 gttgaatatg gagattatat tttaaggtta agttatcatg cttttgttgt gttgaaagaa
     2221 gagcatttca acgagttttt caatttaaaa agtaagtaat catatttttt tgtagacgtg
     2281 tataaagtta actaaattgc gaggtgatga catggtagca aaaaagaata ctctaaaaga
     2341 agcttctgaa aaatatcaag atatgaaaag acgaggatta ataccgaaag acaaaaaagg
     2401 tatatggaga gaatttgtta aggaagtcag tagtgatcga agtggcggtg actttgttgg
     2461 acgatataca gcaaaaggct atagcaaacg attaaggatc actgttgaat atggaacgaa
     2521 tgaatcagct acagagctga agcaaaggta cttagataca cgaagggaag cttcaaaaaa
     2581 atataatgat ttcaagagca gaggtctgat tcctaaaaag aaaaagggaa tttggcgtga
     2641 gtttttcaag gaagaagata gcaatcggaa taagggagac ttcattggac gatacacagc
     2701 aaaaggctac actaagaatt tagggatcaa ggttgaatac ggaactggtg aaagttcatg
     2761 ggaattgaga aatcgctatt taaatacatt gttagagaaa tataaagcag aattattgga
     2821 gttgttcgat gttaatgatt ttatcgagat ggctgaaaag tatggtcatg aagaaggttt
     2881 tatgatcaat gacattatgg ttattgataa ttttacggat ttattagcac ttgcgaaaaa
     2941 agcaagggat ttaagagaaa cgcagatgta tctattcaga aataaaaacg gaagaaaccg
     3001 gagaactgaa tcgagtgaga ttgaagatta ctacgatgcc tttacacacg atctgggagc
     3061 ctttgtagac gagataagtg gtgtaatgaa caaatatagg gtctagaata aaaaagccgg
     3121 agatgagcag ataaatggga aacaatagca tatacacacc tgaaatgcag aataaaatta
     3181 aaagacttgt cggattaggg tggatgacaa gaggaaaatt agattctttt agtacgatca
     3241 aatatctttt gatggacatt taccgtcaga acaagcaaac aggaatacat tttttgggct
     3301 atgataagcc ggaagatgca cctttctttg ttccaagttc agcctataga gccagtacct
     3361 ccggtagcat gtataacttg gatttgtttg aggaaggagc aaagatgatc gaatatgatt
     3421 tcaacgaagc ctattcgagg atcatgagaa attatccttt gccgtcaaac acttatttga
     3481 aagcaaaata taatacagat caagctattt tgagagcgat aaatagacca aaagtaagac
     3541 cgtatgaaaa tttaagaacg tatatgttct ttttggttaa ttttgaagga acagccaaag
     3601 aaggaacata tgctaatcca aaatcacagt cgatgtttgc ggattatggc gaaaatccca
     3661 aaacagctaa tgagcctatt tggttatcgg agattgagct aacacttatc cttgattatt
     3721 atgatattaa agagatggaa gtcctagata gccacacatt tttgacgaaa aaaggattac
     3781 tcaaagaata ttttgaaaga atcgagccat taaaaactga tcctgatacg ttgaagcttt
     3841 ataaaaatct tagaacgaaa gtatttggtg ctatcggaca aaaatcatta agagaaggag
     3901 aaacaagcac cttcgataaa tacagtatgt ataatcgagc gtattcctcc atggtagcag
     3961 gcgtatttag agatcgtatg gttagatatg aacagaaata cgttcatagc gcatatgggc
     4021 tattaaaaat tcggactgat ggattgtatt tcagatccga agtaccagag tttgaagagc
     4081 tgtataaact tggagtagtt aagaaaaaag ttcatacaat atccaaagaa gaagtcgaaa
     4141 aagctttaga atttaaaaac aaaggatttg aaaataaggg ctcctttggg aaatctaaag
     4201 atgaagttaa gggtgttgtg atagacgatg atttcgagtt tgattttgac taaatactct
     4261 ttggaaaaga tggtaggagg taaatatgaa gaagaaaatt gtggaaggaa atgtaggtgt
     4321 tattattcta aaagattcaa ctgactatgc taaatcaatg gcactaaaag acgaaaaagt
     4381 tgcacgcatg gataaagatt tagttgaatt tctgatggat gatacagaaa gattaaaagg
     4441 ggatcagttt ttgagtttaa tgaaagacaa aggcttaaac acatatggtt tatttgatta
     4501 taagaaatta gctatagagt gggtaaaaga aggagaaata ttttttattg aagaatggga
     4561 aggtactgag aacttagttc ttcatcagta tcttttgaaa gcttaatcta agtaaaacag
     4621 ttctgactag ttatgcttgt cagaatttca aaggaagttt tccataacca tcatcattca
     4681 aaaataaagg atttgaaaat aagggttcct ttgggaaatc taaagatgaa gttaagggtg
     4741 ttgtgataga cgatgatttc aagtttgatt ttgactagat ttttttgaaa ggtttaagag
     4801 taaacaaggt aagaactaag atcatgaaga ttgagagtaa agaagataga ggacgaatgt
     4861 atgagaacac actatattta tttagaagat aaaattgtag atgaaattcc ggcaaaaagt
     4921 cgagaacaag ctagaaaatt atttattgaa aaatacaaag agatcaatgg agatagtgaa
     4981 gttccaaaag tggtcgtaac agttagaaaa aggcaaacaa aaaagagaag tacgtttcat
     5041 gtcagccctg atatatataa tcttatagag cgacatagaa gtgaagataa tatttctatc
     5101 tctcaatata ttgctacagt tattgatgaa ttgatttcag gaaagtatga ctatgctttt
     5161 gattttgagg gtgaaaagga ttacaaaagt gttgctgtct ttcaatctga ttttgagaag
     5221 ctaaaaaact atgctggcag gagaaatgtt aattttgcaa gactactaga agagttctac
     5281 tccgaagagt tgggagtagg aaatgaatac aagagagaaa cttatgcgaa attgtctcat
     5341 aagttatccc aagcgcttaa agaagtgaca gaagttaaag cggaactaga tgctaaactt
     5401 gaagaagtga aaaaatatca aacagagagg aagaacaaca tgaagaatca agtggaattt
     5461 gacgaacaga aagaatttga aaaagcaaga aaacgctata acaaaattat gaatgaactt
     5521 agctatgaac acttaacagt tcatgaataa ttgagtgagg gtacagaaaa atatacgatc
     5581 gcagatatgg ttaatgaagt ggaaactctt ttagcttctt tctttgaaaa tggtcacgta
     5641 aacgatgaat ggcgaaaaga agaacctaaa agggttaacg cttatattgc tcgattaaaa
     5701 aggtttattg ataaatataa ggagaatagt gacaaatttt ctaaagataa ataaggagag
     5761 agtgtgatta catttgccag aaatcgaagg aatattttcg ccaaaaatag gtaaaaaaca
     5821 gttagatttc agcagttttc cgagcgtaaa tagagattat aatctaggtg atgaggtaca
     5881 gatactaaat attgatttca aagaactaga cttcgctgtt gctgtttcca acaaacccaa
     5941 ggcattggaa cgagtgaaaa aaatatacca agagaatgat caaaatatca aaaatatgat
     6001 caaaaagtat agacgagata attcaaaagt tgaagacgtt ttattcaacg gtacggcaaa
     6061 acatgatttg aatgtttata tggcattagg agtggctttg tactgtaatt caattaatca
     6121 gacagatatt ttggttaata tcgtgaggaa tagctatcaa aaaatattaa aaataagtag
     6181 cgaaacaaac ccttattatg caatgaaata tcttggggat aatttattaa atgataaaaa
     6241 attgatgcac aacactgaag agctgttatc taatttggct gcgattaact ttctattcaa
     6301 aacaggttct gaagattatt ttatagataa cgtactaaaa ggtgcttacg gaacagcatt
     6361 ttatccgctt tacaatatga aacagcataa agccgaacaa aaggataaca aacttgaaat
     6421 taaaaagatg aaagaactat taggtatagg atttgtaaaa cccaattcaa aagtcactat
     6481 tgcttcgtta gcaactacta acaatgatgg gaaaatagta aatgggtttg acgattcaga
     6541 tatagatata ttgtcaaaat catatttttc tttgtatgga gattttgcag atttgatggg
     6601 tattgatagt tacttagtca ataattttga gatcaagggt agcgagctga atgatattgt
     6661 gtatttcgtt ttaatctctc tatatgcaat tgatcagaat accgagtacc gacaaattga
     6721 ctatttgcta gttttttata gtttgttata tcagttcgtt ttaactcggt tgtttaaaaa
     6781 tgctaaggaa gaattgatcc tgaattctga ttatgaaata gcaaaacaaa ttgcaagtat
     6841 tagaaaagaa aacaaatctg aagtaatcaa attaagaaaa gaattaagta ttgcgagtaa
     6901 agaaaacggt cggttagaat ctttagtcaa atcggcaaaa aaagaagagg aacttttgaa
     6961 gcaagagatt aaagaattaa aaggaatcag aaaagaactt gaacagctga ggtttgaaaa
     7021 taaagtatta aagcaattgc aggagcaaga acatagacaa tcattaaatg gtaaaactga
     7081 agagccttca ctgtctgaaa tgatagctta tctaaaacat aaaaacattc ttttagtggg
     7141 aggacatgta aattttcaaa ataaattaac tgaactatta ccaaatatta aaatgtttga
     7201 tgtaagcgaa aataatggaa agatacttaa aaatttgaat acgtgtgatg cgatttttct
     7261 atatacagat tacttgaatc atggtctata ttatcgtgtt atgagtgaaa tttctaataa
     7321 agataagata ttttatttgg ataatcatgt gaatatgaac ttaacaatta aacgtattta
     7381 cgatctttta tcacttatat aaagagagaa gtgataaata ggacacaaaa tttggaaagt
     7441 aggtaagtca ctatgagaga actaacttta tcttctaagg aattagaaaa gaaaactgct
     7501 ttattaaacg aaatatttag tgctgattgt ataatccaaa gtttaaaaga aagttcattt
     7561 tcaaaactaa cagatcagga aaaaatttct tatatgctaa gtttagataa tctaggaact
     7621 aaatatgatg aatcagcaag tagcgataag cttttgattg aattagtatc aagtcttagt
     7681 agttttggaa aaatgaaaag atttaatgtg atattagaaa tttttgcttg gttaatccct
     7741 tcatttattt ggttttttta tattttcata gttgaatctg agttaatagc caaggtctct
     7801 cttggactgt taattccgat ataaataggt tcagcaattt actttaataa cttgtataca
     7861 cctattcaaa taaaaaagca gttattacat gaaggattaa gtggtaatat gttaaaaaga
     7921 attgcatggg ataaagataa taaaacagat attgagctta aagaatataa atacaattat
     7981 ttctcgagat gtatagatag aaagttactg tttaaaaaag aaggattaga atttagtttt
     8041 gatgaagatg aatttgaaaa attagtgatg gactggcgta agaatttact ggatacggta
     8101 caattctaaa agcaattgag gtcgaaattc cgatacttga atcatgatct atattatcgt
     8161 gttatgagtg aaatttctaa taaagataag atatttcatt tggacagtca tgtgaatatg
     8221 agcttaacaa ttaaacgtat ttatgatctt ttatatgttt ataaacatta atgaggtgct
     8281 aaaattgaca acaataacta tgacaaaaaa aatagaagta tatgttgatg gatcacaaga
     8341 agaaagggat tccttttttg attttttttg gaaaaatagt agagaatatg taaaagcgtt
     8401 gaatctggta tataaacagc taatgtttga agagttgata aaagatgcaa ttaaagaaat
     8461 ggatcaaggt tatgctgaga gagaagaaag tattcaaaat cggattgaaa agttagcata
     8521 tactgatcca atagatcaaa aaaaacttga taaagcaaaa gctgaattat ctaaattgag
     8581 aaatgaaaaa aataaagaag cgagagaaat attaacgcaa gcgatagggt tgaagagtca
     8641 aacgagaaca cgagatgtag caaaaaaatt agactttgac tttggagatt ttgttgattc
     8701 cgcaaatatg aaagctagta aagattttgg caatgatttt atcggaattg tgaccggaca
     8761 aagaacgcct agaatatata aaaatagaaa caaatatgga ttattacctt ttagaggtcg
     8821 agatattaaa atccataaag atgataagtt ttatatttat ttacctaaag gctttaggtt
     8881 taaagtgaat ttaggtagca agccaagaaa agcaagagat gttgcttcag ttttacaaca
     8941 cattatggat ggcgattaca aattaaatca aagtaatctt atttttgatg gaagaaaatt
     9001 attttttaat ctaacaattt cttttgaaaa aagctataat gaaatggaat tcaagaaagg
     9061 taatgaattg atcgtaaact ttgcagattt gtgtagcggt gaatgtttaa ttaatgataa
     9121 atctgtaatg tcttttggtg atagccaata tataagaaac ttcaaaagta aaattttagc
     9181 aatgaaatca aaagaacaaa gccgaggagt cttttctaag ggcggtcatg gtcgaaaaag
     9241 aaaattagat caagatcgat gggaagcaat aaagaaaaga gaaagtaatt ttgttaaaac
     9301 ttataataat caatgttctg caaaaatcgt taaaactgct attcgcaata aagctcaaat
     9361 tattcgtatc attgaaccag ataaggctta tggcgaatgg gcttactatc aattgaaaga
     9421 acaactagta aacaaaggaa aaagatatgg gatagaagtc agaagttagt aaattaaaca
     9481 atcgttgatt tggcaacgaa atagtgaggg aaaatccact cactgaaggg taaactccgt
     9541 caagttgtgt gaccagacgg aaggacgaat gttgttgagg ttttagaacc atataaaaca
     9601 gcatgtttct caaattttta ggataaggga aattgctagc agtttgtttt agaaccatat
     9661 aaaacagcat gtttctcaaa tgagtacttt aaattaattg atggcgttcc tgctttagaa
     9721 ccatataaaa caacatggat ttaaaacaag aagtaagtca gttctccttt tactttagta
     9781 aggaaaaata aagtattctc aataaaactt aaaataagca aacctttgtt ttaaaatgat
     9841 tgtcaagtta aaaaaatctg ggatcatata aaatagtata gttctcaaat atgattagaa
     9901 accctattaa agagcaatat taagccaact tacttaaatc gtttaattaa gaacaataat
     9961 ctcttttagt cagactttta gtctgacttt tttgtatttc caaaatttat tctttacaaa
    10021 aacgaacata cgtttgtata attacttgtg aatgggggaa taaaaatgtt tgattacagt
    10081 agagaaccgg tgaatgacta ttttttaatc gacatgaagt ctttctatgc tagcgtggaa
    10141 tgtattgaac gaaatttaga cccaatgact acaccgttaa ttgtcatgag tagatcggac
    10201 aacactggtt caggtctgac tctagcttct tcacctactg caaagaaaaa atatcatatt
    10261 actaatgtta gtagagctac tgatttacct tatccattac caaaagaact tcatattgtt
    10321 ccaccacgaa tgaacttata tataaaaaaa aatatggaag ttaatcagat ttttagaaag
    10381 tatgtcgcag atgaagattt attgatttat tcgattgatg aatcaatttt aaaagtgaca
    10441 aaatccttaa atttatttac tatataaatt gagttaaatt gtattcgttt ttcctcctat
    10501 aggcgtatac taaagaaaac acttgaggag tggggaacat gga
//
LOCUS       DANKEF010000077        10493 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000077 DANKEF010000000
VERSION     DANKEF010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.077"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..357)
                     /locus_tag="PWZ48_002431"
     CDS             complement(<1..357)
                     /locus_tag="PWZ48_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2262068.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
     gene            complement(709..1068)
                     /gene="rplT"
                     /locus_tag="PWZ48_002432"
     CDS             complement(709..1068)
                     /gene="rplT"
                     /locus_tag="PWZ48_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDL2262069.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="PWZ48_002433"
     CDS             complement(1121..1321)
                     /gene="rpmI"
                     /locus_tag="PWZ48_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDL2262070.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(1393..1917)
                     /gene="infC"
                     /locus_tag="PWZ48_002434"
     CDS             complement(1393..1917)
                     /gene="infC"
                     /locus_tag="PWZ48_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDL2262071.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1961..2086)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            2237..2869
                     /locus_tag="PWZ48_002435"
     CDS             2237..2869
                     /locus_tag="PWZ48_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDL2262072.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2871..3776)
                     /locus_tag="PWZ48_002436"
     CDS             complement(2871..3776)
                     /locus_tag="PWZ48_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HDL2262073.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3861..4520)
                     /locus_tag="PWZ48_002437"
     CDS             complement(3861..4520)
                     /locus_tag="PWZ48_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HDL2262074.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4836..5780
                     /gene="mvk"
                     /locus_tag="PWZ48_002438"
     CDS             4836..5780
                     /gene="mvk"
                     /locus_tag="PWZ48_002438"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HDL2262075.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5773..6750
                     /gene="mvaD"
                     /locus_tag="PWZ48_002439"
     CDS             5773..6750
                     /gene="mvaD"
                     /locus_tag="PWZ48_002439"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HDL2262076.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6763..7848
                     /locus_tag="PWZ48_002440"
     CDS             6763..7848
                     /locus_tag="PWZ48_002440"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HDL2262077.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7845..8909
                     /locus_tag="PWZ48_002441"
     CDS             7845..8909
                     /locus_tag="PWZ48_002441"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDL2262078.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(9158..9961)
                     /locus_tag="PWZ48_002442"
     CDS             complement(9158..9961)
                     /locus_tag="PWZ48_002442"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HDL2262079.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="PWZ48_002443"
                     /pseudo
     CDS             complement(9981..10286)
                     /gene="kduI"
                     /locus_tag="PWZ48_002443"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(10268..>10493)
                     /locus_tag="PWZ48_002444"
     CDS             complement(10268..>10493)
                     /locus_tag="PWZ48_002444"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HDL2262080.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         3104 a         2220 c         2008 g         3161 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa aaaaagagga
      661 aagaacataa cttttcgtta tcttctttcc tctttataat tgtttagctt atttagccaa
      721 agcttctttt gcttggtcag ccaatgcagt aaatgctgct gcatcgttaa cagccaagtc
      781 agctaacatt ttacggttga tgtcgatttc agcaactttc aaaccgtgca ttaattttga
      841 gtagctcaag ccattcatac gagctgctgc gttgatacga gcaatccaca atttacggaa
      901 gtcgcgtttc ttttgacgac gatcgcgata tgcgtagttg tatgaattca ttacttgttc
      961 ttttgctgtt ttaaataaag tgtgttttga accgtagtaa ccttttgcta attttagcac
     1021 tcttttacga cgtttacgag ttactgttcc acctttaaca cgtgccatgt ataattcctc
     1081 ctaataaaaa taaatcttca gattctgtta tttgtacttc ttatttcatt tttgctagtt
     1141 gttggcgaat acgtttgaag tcgccactag aaaccatgct tgctttacgc aattgacggc
     1201 gttgtttttt tgttttgccg tggaaacggt gacttgtaaa cgcgcggaag cgtttcaatc
     1261 cgcctttacc agtacgtttg aaacgttttg ctgatccgcg gtgtgttttt tgttttggca
     1321 tgactatatt tcctcctcaa aatctttcgt tatccgacta tcggctgtcc aacagccagt
     1381 tagtcattca aattacttac tgtcgttttt cggtgccagc gtcaagaaca tgctgcgtcc
     1441 gtccattttc gctttttgtt ccactgttgc aatatccgca gtttcttcag ctaagcgatt
     1501 caagactttt tgaccaattt ctttatgggt aatggcacgg cctttgaaac ggatagaagc
     1561 tttcacttta tcgccctttt ccaagaactt acgtgcatta cgaagttttg tattgaagtc
     1621 attcaagtcg atcgtaggac ttaagcgaac ttcttttaca ttgatcactt tttgtttttt
     1681 acgcgcttcg cggtctttct tttgttgttc gaaacggtat ttaccgtagt ccatgattcg
     1741 cgcaactggt ggtttcgcac caggggcaac taacacaaga tccaagtttg cttgttctgc
     1801 aatattcaat gcttctgctt ttgtcttaac tcctaattgc tcaccatctg aaccgatcaa
     1861 tcgtaactct cgtgcacgaa tgccgtcgtt aaccatcata tcctttgcta tggtcattca
     1921 cctccaaata ttatgagaga aagaaaagct taccgaataa acggcaataa aaaaacgagt
     1981 tctcttacag aacccgcgcg tacttcacct aatagaacca gtgaaaacta tctagcccag
     2041 tgacattatc aactaaggcg agaagcgggt gcttctgctt ttatttctca accattatag
     2101 tatagcaaaa actaatcttt ttgtcaatgt ttttcataca gtttgtcttc cttttctgaa
     2161 aatgattaga ttagcaggct ttctagcagt gattcccgct tctttgctat actaaagaaa
     2221 aacgaggtga acccacatga aaaaaatcga acctattcca ccttctcttc ctcttcttac
     2281 aaagggagag ttttatttag aagatgagga gatcttctct ggtcttctga tcaacggagg
     2341 taaccaaagc tatctcgatg ccaaaaatat ggtattaaga gagagtcata tccaaaaact
     2401 tacgatgcaa aaaacaggtt tacgtcattt tgaatgcagt aatgtcattt ttgaaaaatg
     2461 tgatttttcc aatctggagt ggattggtgg cagtttccac caagtcgtgt ttcatcaatg
     2521 taaattaaca ggtacgaatt ttgccgaaag ttatcttcga gactgtactt tcactgattg
     2581 tattgcttcg atggcttcct ttagtagtac aaacttgaaa gctgtctcct tcgatcattg
     2641 tcaattagaa gacagcgaat tttatgaggt cacttggaaa aacctttttt tgtctgacaa
     2701 tcagttaacc ggttcgaact ggtttcgaac ttctctgaag ggattagact ttacgactaa
     2761 tttcttcaca acaatcgctt tatctcaaga ttctttggcg ggactaatcg tcaatcaaga
     2821 gcaagcactt gtgatcgccc aagcccttgg tttgcagatc aaagactaga tcaacgaatc
     2881 cgccgccaca gtgcaataag catccaaata gataagacca aactgatgat caaggcgatt
     2941 tcccatccga aaacggaatg ggtaaagggt aactggagat tcattccgaa gaatcctgtc
     3001 acaatagttg gaacggtcaa gatcagcgac cagaccgtca aaaatttcat cgtgtcattc
     3061 aagttgttat tcagcaaatt attataagtg ccagagagct ggtctaaaat ctgcgaagct
     3121 aagttcgtca tttcaactgc ttgtcgtgct tcgatcagtg catcgtccaa ctcttctttt
     3181 tccttttctg acaacttgcg ataaatagcc agtgccttga tttgttctaa aagtactaca
     3241 ttttgttttg ttcctgtaac taagtaaacc aaaccgatct ctaaatctga aagttgcagc
     3301 agattcttgt tcgttgtttt ttctcttaat ttcagattaa gccgctgacg ttcactattc
     3361 acttcctcga ttaaaggaaa aaaggaatca gaaatcaaaa acaatgtacg aaacaataaa
     3421 ctgtatacac tcatgtctgg catccgttcc aaaagcgatc gaattagtcg aatcacgtac
     3481 tcggtattcg gcgtggtaaa tgtaaacaaa ccgtcttctt ttaaaataaa agcgatagga
     3541 ctagtttcat aatgattatc gactttttgc tggtgcggaa catttgatac aagcagtaac
     3601 gcttcatcaa aagaatcgta ctctacgcgc gcccgttcat ttttatctag agaataagtc
     3661 agcatctcgt cactaatgcc atattcttct tttaagtttt taacagctag cacatcgttt
     3721 tgctgtacat tgatccaaaa attacggtct tgattaaaat agtgtttttc ccgcatactc
     3781 tatcctcctg acatttgttt ccagtataaa gaaaaaagtc gctcaaataa aggtttaccc
     3841 tttatttgag caactgtaaa tcaaatggat actttggttt ggcgaatctc tgctaatttt
     3901 tcttcgatca ttttcaagac ttcttctttt gcttgaggat cttccacaaa attatagcgg
     3961 tctccatcga tttggatttt tggactttct ttgtattctt cataccattg atcataacgt
     4021 ctgttcaatt ctttgtaata atcgtataga cttgggtcaa aggacaattg ttcatacgaa
     4081 cgaccacgtt tttcgatacg ctctaacatc gtatcaaaag atacacgaat atgaactaat
     4141 aaatccggat gttttttatg ggcagcatag ggaagttctt ccatcatgtt ttccagcagt
     4201 tcatcataca cacaaacttc tgtttctgtt gctcttccta gatctgcatt tagatggaaa
     4261 agcagtgaat cttcataaat cgaacggtcc aatacattat tgtcatcttt cattgcttgt
     4321 ttgatgcttg caaaacgttt attcaaaaaa taaatttgca gcaaaaaagc atactgttca
     4381 gggtttgcat aaaataaggg caagacttca ttatcatcaa tcgattcata aaatgcctga
     4441 ctatcaaagt gttcagccat catttctgtt aaacttgatt ttccggcacc aattgtacct
     4501 gctaaaacga tcacgctcat ttacctccag ctcacttcat cactaaatat tgtgtttaac
     4561 tccaaccttt tttaaatata cccccaatat ctagtagtgt caattgtatt ttttctagtc
     4621 aaagaagaaa aaaagtcaaa aaattcttaa gaaaaaagtg ttttttcttt agaaaacgta
     4681 ttcttagttt tacttctgag atggttttag taaaataaag gaggaatgtt ttttgagacc
     4741 cttagtggac aaaaaaattc gtgaagcagc gcaactcgtt ttgggcgctt tattttttgc
     4801 ttaaaaatca cttttacgtg agaaggagtt agattatggc aaactatggc caaggagaat
     4861 caagcggaaa gatcatattg atgggcgagc acgcggttgt ttatggagaa ccagcgattg
     4921 cctttccttt ctatgcaaca aaagtcaccg cattccttga agagctggat gcaatggacg
     4981 atcaactggt ttcttcctac tattcaggaa atttagccga agctcctcat gcattaaaaa
     5041 atatcaaaaa attattcatt cacttaaaaa aacagcatga catccaaaaa aacttgcaac
     5101 tgaccattga aagcacgatt cctgctgaac gtggaatggg atcaagcgct gcagtcgcca
     5161 cagcagtcac tcgtgctttt tatgattact tagcatttcc tttgtctcgt gaaatactat
     5221 tagaaaatgt ccagctttcg gaaaaaatcg cccacggtaa tcctagtgga atcgatgcag
     5281 ccgctactag cagcttgcag ccgatttatt ttacaaaagg gcatcctttt gactactttt
     5341 ctttgaacat cgatgctttt ttgattgtcg ctgatacagg aatcaaagga caaacaagag
     5401 aagccgtcaa agatgtcgct cacctctttg aaacccagcc tcatgaaact ggacaaatga
     5461 tccaaaaatt aggatacttg acgaagcaag caaaacaagc aatcattgag aattcaccag
     5521 aaacgttggc acagacaatg gatgaatcac agtcacttct ggaaaagctg acaatcagca
     5581 atgattttct taatctattg atccaaacag caaaagatac cggggcattg ggtgccaaat
     5641 taactggcgg tggacgcggt ggctgtatga ttgcattagc acaaacaaaa acaaaagccc
     5701 aagaaatcag tcaagcactt gaagatgcgg gtgctgctga aacttggata caaggattag
     5761 gagtacatac ctatgtttaa aggcaaagca cgcgcatata ccaacattgc tctaatcaaa
     5821 tattggggta agaaaaatga agaactcatc cttccaatga acaacagcct ttcgttaaca
     5881 ttggatgcat tttatacaga gacagaagtc atcttttctg atagctatat ggtagatgaa
     5941 ttttatctag atggcacctt acaagacgaa aaagcaacaa aaaaagtcag tcagtttctt
     6001 gacctttttc gtaaagaagc cggcctctca ctaaaagctt cagtaatcag tcagaatttc
     6061 gttcctaccg cagctggatt agcctcctct gccagcgggc tagctgcttt agcaggagct
     6121 tgcaatactg ctcttaagct tggattagac gatctctctc tttcaagatt tgctcgacgc
     6181 gggtctggtt cagcttgccg aagtattttc ggtggtttcg ttgaatggga aaaaggccat
     6241 gacgacttaa gttcttacgc taagccagtc ccttccgatt ctttcgaaga cgatttagca
     6301 atggttttcg ttttgatcaa cgaccagaaa aaagaagtgt ccagcagaaa tgggatgcgt
     6361 cgaacagtcg aaacatccag tttttatcaa ggctggttag attccgttga aggggatcta
     6421 tatcaattga aacaagcaat caaaacaaaa gatttccaac ttctcggaga aacgatggaa
     6481 agaaacggac taaaaatgca tggaacgact ttagctgccc agcctccatt cacttactgg
     6541 tctcctgaca gcttaaaagc gatggatgcg gtgcggcaat tgagaaaaaa aggcattcca
     6601 tgctacttta cgatggatgc tggccctaat gtcaaagttt tagtggaaaa cagccattta
     6661 tctgaggtac aagaaacatt taccaaatta tttagcaaag aacaagtaat tactgcccat
     6721 gctggtccag gaattgcgat tattgaataa agggagattt ttatgattga agtatctgca
     6781 ccaggcaaac tctatattgc cggagaatat gcagttgttg aaacaggaca tccagcagtt
     6841 atcgctgcag tcgatcaatt cgtaacagtc actgtagaat ccgcacgaaa agtcggaagt
     6901 atccaatctg cacaatatag tgggatgcct gtacgttgga caagacgcaa cggagaatta
     6961 gttttagata ttcgggaaaa tccttttcat tatatccttg ctgctattcg tttgactgaa
     7021 aagtatgcac aagaaaaaaa cattctttta tccttttatg atctgaaagt aacaagtgag
     7081 ttagacagtt caaatggccg gaaatatgga ttgggttcta gcggtgccgt gaccgttgca
     7141 acggtcaaag ccttgaatgt tttttacgcg ttgaatttat ctcaattgga gattttcaag
     7201 attgcagcac tagccaattt agcagttcaa gataatggtt cctgcggcga catcgctgct
     7261 agctgttatg gtggctggat cgctttctca accttcgacc atccttggct ccaagaacaa
     7321 gaaactcagc attctatcag tgagttactt gccctggatt ggccaggtct atccattgag
     7381 ccattgattg ctcctgaaga tttacgttta ttgattggtt ggacgggtag ccctgcctct
     7441 acttctgatt tggtcgatca agttcaccgt tcgagagaag acaaaatggt ggcttatcag
     7501 cttttcttaa aaaacagtac agaatgtgtc aacgaaatga tcaaagggtt taaagaaaat
     7561 aatgtaacgt tgatccaaca gatgattcga aaaaaccgac aattactgca tgatttatct
     7621 gcaatcactg gggtcgtcat cgaaacgcct gctttgaaca aattgtgtaa tttagctgaa
     7681 cagtatgaag gagccgcaaa atcttctggt gcaggtggtg gcgattgcgg aatcgtaatt
     7741 gttgaccaga aatctggcat ccttccttta atgagtgcat gggaaaaagc agaaatcact
     7801 ccactgccgt tacatgtcta tagcgatcaa agaaaggaaa accgatgaat cgaaaagacg
     7861 aacatgtttc actagctaaa gcctttcatg ataaacaaaa aaacgaattt gacttcgtac
     7921 ggatcatcca taaccctttg ccgcaaatag cagtttctga tgtcgatcta agtacacagg
     7981 cagttgggtt tacactaagc agcccatttt atatcaatgc gatgactggc ggaagcgaaa
     8041 aaacaaaaaa aatcaaccaa gatcttgcaa tcgtcgcaag agaagcagat ttgatgattg
     8101 ccactggttc agtcagcgct gccttgaaag acccttcttt agccgatact tatacgatta
     8161 tgcgacaaga atatcctcac gggaaaatca ttgccaacat cggtgcaggg acttctgtcg
     8221 aaagagccca agaagctatc cgattgtttc acgcagatgc cttacagatt catttgaatg
     8281 cacctcaaga gttggttatg cctgagggag accgtgattt taccaattgg aaagtactta
     8341 ttcaagagac tcaaacagcc attgacgtcc ccctcatcgt aaaagaagtc ggttttggca
     8401 tgacaagaga aacgctcaat gatctagctg ctttaggagt tcatacagtt gatatcagcg
     8461 gtcgaagcgg aacaagcttc acccagatcg aaaacgcccg tcgctccaag cgggaactga
     8521 gttatttagc tgactggggg caatcgacag tctcttcatt gcttgaagca aatgaagcag
     8581 acacttctat ggaaatccta gcctccggag gtatccgcaa tgcctatgac atatttaaag
     8641 ccctttgtct cggcgcaaat gcagtgggta cttcaggaac cgtactgacg catctgatga
     8701 atcacggcgt agaagaaact attatactga tgaagcaatg gcaagaagag cttcgtctgc
     8761 tttatactat ggtcggcgca acgaataccg cggccctaca ccaacaatca ctgatctttt
     8821 ccggaccagt gaaggattgg tgcgaagcaa gagggatcga cttagtgaag tatggaagac
     8881 gcacagaaaa gagcgtggga caaaagtaaa gaacactttt atcccacgct ctaaacacgt
     8941 ataaacggcg gaagcaaaag caactccttc ggaaataagc tgaaattcac aaaaatttga
     9001 aaagcaattt tcgtgaattc cttcttattt cttggagtta aacgcttctg tcccagcctc
     9061 ttttttcgaa taaatgatgt ttaatcagct gtccttcggt attcgttcaa gccgctgatt
     9121 tcacaacctc tccactaaac ggtgtactaa agctctttta aggttgtttt ccgatatagg
     9181 caagtatgcc tccgtccaca tacaagacat ggccgttaac gaaatcagat gcgctagaag
     9241 ccaagaagac agatggtcca gccaaatcga ttggctcgcc ccagcgtgct gctggcgtac
     9301 gtccaacgat aaattgatcg aatggatgac gttcaccgtt ttcttgtgtt tcgcgtaatg
     9361 gagcagtttg cggtgtagca atataacctg gtccaattcc attacattgg atattgtatt
     9421 gaccgtattc agaagcgatg tttttcgtca gcatttttaa tccgcctttt gccgcagcat
     9481 aagcactgac cgtttcgcgt cctaattcac tcatcatgga gcagatattg atgattttac
     9541 cgccgccttt ttcgatcata tcccgaataa ctgcttttga aacgatgaat ggggcattta
     9601 aatccacgtc gattacttgg cggaagtctg ccgcatccat ttcgatcatc ggtgtccgtt
     9661 tgatgatccc tgcattgttg actagaatat caactgaacc tacatcttct ttgatctgtt
     9721 gaaccatcgc ttgaaccgct tcttcatcgg tcacatcgca gacataacct tttgcatcga
     9781 tgcctgcttc tttataatgc gctatacctt catcaactga ttcttgattc aagttattaa
     9841 atacgatttt tgctcctgct gtagctaaag agcgtgcaat ctcaaatcca ataccataga
     9901 cagcaccggt gactaatgct acttttccat ctaaacgaaa catatccata ctaaattcca
     9961 tcattttctc tcccttatgc ttattttaat tgatccattt gcaccatatc catgtctgta
    10021 taggtgatat tttctccgca cattgcccag atgaatgagt aattgcttgt acctacacct
    10081 gaatggatcg accagcttgg cgaaataatg gcttgttcat tgtctacgac taaatgtttt
    10141 gtttcatctg gtttgcccat catatggaac acacgtgtat cttcattggc ataatcaaaa
    10201 tagacataag cttccatacg acgttcatgt gtatgacaag gcatcgtatt ccaagcactt
    10261 cctggttcta aaatcgtata acccatttat aaatgacagc tatcgctgaa gcttctactg
    10321 ctgtaaatat ccccgtcaag ataccaccga taatgatgac aatcaataga aggctaggaa
    10381 tcgcctcttt tattattttt ccagcatgag ccatctgtcc tttttcgaca acagggtatt
    10441 tattcttttt agcaacgatg aatgctacta gcatgattcc tagcgcccac aaa
//
LOCUS       DANKEF010000078        10273 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000078 DANKEF010000000
VERSION     DANKEF010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10273
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.078"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(66..1574)
                     /locus_tag="PWZ48_002445"
     CDS             complement(66..1574)
                     /locus_tag="PWZ48_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HDL2262081.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATCLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            1733..1924
                     /locus_tag="PWZ48_002446"
     CDS             1733..1924
                     /locus_tag="PWZ48_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262082.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            2127..3044
                     /locus_tag="PWZ48_002447"
     CDS             2127..3044
                     /locus_tag="PWZ48_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HDL2262083.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            3037..4110
                     /locus_tag="PWZ48_002448"
     CDS             3037..4110
                     /locus_tag="PWZ48_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262084.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            4258..4776
                     /locus_tag="PWZ48_002449"
     CDS             4258..4776
                     /locus_tag="PWZ48_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262085.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            4757..5149
                     /locus_tag="PWZ48_002450"
     CDS             4757..5149
                     /locus_tag="PWZ48_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HDL2262086.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            5225..6160
                     /locus_tag="PWZ48_002451"
     CDS             5225..6160
                     /locus_tag="PWZ48_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HDL2262087.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            6293..7072
                     /locus_tag="PWZ48_002452"
     CDS             6293..7072
                     /locus_tag="PWZ48_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HDL2262088.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            7065..7382
                     /locus_tag="PWZ48_002453"
     CDS             7065..7382
                     /locus_tag="PWZ48_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HDL2262089.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            7626..9236
                     /locus_tag="PWZ48_002454"
     CDS             7626..9236
                     /locus_tag="PWZ48_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HDL2262090.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            9393..10241
                     /locus_tag="PWZ48_002455"
     CDS             9393..10241
                     /locus_tag="PWZ48_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HDL2262091.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT         3235 a         1800 c         2071 g         3167 t
ORIGIN      
        1 ataaaaaaac gaaactatcg attaagctaa tagtttcgtt ttcaacattt ataagtaaac
       61 tttttctatt ggtaatatag attttcggaa gggtagacag gtgcgcaggt aatatcgatt
      121 cctagttttt tcaatgtttg ttcatcattt ttattgacca tcacagtaga gtgggcttgt
      181 gctccctcaa gttcagctag tttatcataa gcaacttgag cgattggatt cgttactgca
      241 ctgattgata aagcgatcaa gacttcattg atgcttagtg catccaaaga ggaatgaagt
      301 gtctttgatt tcaatccttg tattgtttca agaactaatg gtgacaataa aggaatgtca
      361 tctgcgatat gggctaaatg ttttactgca tttaagataa ctgccgctga cgcatccatc
      421 aatgaacttg tacgcccggt aatgatgact tcatctggta attcgattgc aatcacagca
      481 gaaggatctt ttgtaggact ttgcgcttga atcttatctg catagtcatg ggcagggact
      541 accacgtttc ggtcttcagg acgtaaaccg acttcttcca taatcagttt gattcgatta
      601 actgcttctt cgtcgatcaa tccttttttg aagtcgcatt ctgtttggaa ataacgacgg
      661 atgatttctt gtttagaggc ttcttggaca acggtatcat ccacgatccc aaatcctaca
      721 cgattcacac ccatgtcagt aggagattga taaacagatt ctttaccagt aattttttca
      781 ataatacgtt tgattacagg aaatgtctca acatcccgat tatagttcac agctacttta
      841 ttgtaggcat caaagtggaa ggaatcgatc atattgacat cttttagatc gactgtggct
      901 gcttcataag caatgttcag cggatgtttc aacggaacat tccatactgg gaaagtttcg
      961 aacttggagt aaccagctgc atgtcctttt tggctttcat gatacagttg gttcaagcaa
     1021 gtagcgagct ttccacttcc tgggccagga gctgtcacta caacgattgg tttagttgtt
     1081 tcaatatact cattttttcc aaaaccatct tctgagacga ttttttctac gttgactgga
     1141 tagtcttcga tttctgagtg tttatagacc ttgatgtttc gtctttctag tttattgata
     1201 aagactttcg tggcaggctg tccactatag cgagtgataa cgacactgtt gattaataag
     1261 ccataaccac gtaattcatc gattaaccgc aagatatcca tatcataagt aatcccataa
     1321 tcaccacgga tcttattacg ttcaatatct ccagcataga cgcaaatcag tatttcagct
     1381 tgatctttca atttttgcag caatttgatt ttcgcatctt cttcaaatcc tggaagtaca
     1441 cgtttcgcat gcttgtcgtc aactaacttc ccaccaaatt ctaaatataa tttgtcatag
     1501 tggttcactc gttccaaaat atacgctgac tgctcttcaa tatatttttc tgaatcaaaa
     1561 ccaatttttt tcactaggtt ccctccttaa gtactaaagc attttttcat ttttttgagg
     1621 attcgtcaac tggaagagcg aaattcctca aaaaaacaaa gaaaatggtt taaaccaact
     1681 tgtctagata gttattcatt ggtatgatag tttttgataa ggaaggtgct acatgaaaaa
     1741 aagacagaaa aagaaaaacg catataaaca ctatatccgt tcgattttta ccggttatga
     1801 aagaatgctt gaagatcccg aattggaaca attgacgttt acttatctaa acgaagaaac
     1861 gcagctaact cgtgatgagc atcagcgcat tcatttcaca acaagagatc tgcctagtaa
     1921 ataaaaaata agataaaggt catcggattc gattctgtcc tatcagatgg aaagactagt
     1981 tgatcgaaaa aaaggctgta ccaaattttg gtgcagcctg ttttatggag cattgcccgc
     2041 tcttttttat aacaattttt tacagaaaat ggtactattg aagtattaat aagtgaaaaa
     2101 cagatgagta agaggaggca agtagtatga aagagccaga tacaaatgca aattatgagc
     2161 cagacgaact agaggagacg aacgcggatc aggatacagg acaagaatcg tcttcttttt
     2221 acgatatcaa agaaaaagca agcatgaatc tttcccaatt attagaagac ttacaggctt
     2281 ttgaaaaagc tgtccgcgaa gaagatatcc ctgagatcta ccgtttatac aatggccgat
     2341 tgaatgaaga attaaaaaga agctcaaatg aaaatcacga gatcgatcaa ttgctgatgc
     2401 gtaagatcca tgatcgtttt ttacaggaat ttccttttat ggaacaagta gaaaacatct
     2461 cgccaacgat gagctattac aaaatcggca cttattacca tgatcggccc acggtcggta
     2521 ttgatgcaag tctgccagaa atttttgttt tgcctaagat cgatgaagag tgggagaaat
     2581 attcccaacc ggagtcaacg gactatgaca aaaaaatcaa tgagctagac gcaaaggtga
     2641 tcactgctca gacagaaatt gaacgtttag atgaacaaat aaaagaaatc aatcgacaaa
     2701 tgactgattt gaacgacaat aaaggattct tgaatcgtaa aaaaatcgag gaagaaattc
     2761 aagaattaga gaagaagaag caagtactat ctaatgaaaa attaggctgg cttccttata
     2821 ttgagacgcc tgaaacaatc cagcagcaaa aagaagcatt gaagcaagaa gcaagagcag
     2881 accagcttcg tgcagccatc gtagaaaaag aacaaaggca aatcagacgc tatttcggta
     2941 gcaaagaggg attcggtcaa gcaatccatg aattcttgat gaattatctt ggcaatgaga
     3001 atcctaataa tcaatctaat gaaggaggtt ccgagtatga atgaacacga tgaagcagaa
     3061 tggacaaaaa atgaagaaca aacagaagat catgaagaaa cgaacacttc attggaagaa
     3121 caaacaagta atcctctgga tgacacagaa gaaatcccta tgctagagga aacaaaaata
     3181 catgaaaaag aaaaagtaac tccgacgaat acgaatactg cagaaaacac ccatgcttac
     3241 gaaataactg aaaatcccgg gtatttagaa ggaaaagagc aagatcatca agatatcgtc
     3301 agtttgattg aaacagcgga agcagaatta gcggatttac gtatgagaaa agatttgatc
     3361 gaatcagatt ttccacaatt attagaatca tatgatcgtt ttatattgaa tgaagaacca
     3421 atctccacga tcggaaagac aagcctgatc gaatacttca cgtatgaatt actgaaaccg
     3481 ttgaatgata ttggtctgga acggtcaaat tcagcttatg acacatggaa aacaagacat
     3541 ttttcgatca attatacgtt gaatggacaa aacgaattag tcttttcctt tgtattaccg
     3601 accatcgatc aacgatacca agtggaatcg atgagtctcc taaacgtatc accagagaca
     3661 atggaaatca atgtccaaga tgaccgggtg ctttctttga tccgacactg gtcagtggat
     3721 cgagtattct ctgcagggca gatcacgata ttcaatcaca aattgaatca attattagcc
     3781 catgcaagaa cacttggttt taccgttaat cagacattac tagacaatac gaagccactt
     3841 cgtttgaatc ttcagagcga atttgaattg acgaatgaag tattggatga tatttttatc
     3901 gtggcaatga aaaatccatc ctacgatatg gaaaagatca acgaaactac ttatcaagta
     3961 cgattagata aaggacagtc tttaacgata acaacaaacg aaaaagatca aacagtcttg
     4021 tcgatctcat ctggagacta cccacgatct gtcattgatt tctttatcaa ttacgaattc
     4081 ttggtcccat taatggtgag aaagtcctga ttggataagt tggattacca gatgtcattc
     4141 agataattct gttccaaagg acttgcggca gttatccaga ataattgtca caagtctttt
     4201 ctgtatgtac taaatggacg tgaataactt gctgatgaag gagggatttc attaggaatg
     4261 tccacaaata aaatagcaca gcttagctta ttgtctgctg cttgtgtagc aggtaggatc
     4321 gcttttcaat ttattccgaa tttccagcca atgactgcta tttttttatt tatagtactt
     4381 tacttaagct taaaagatgc gttaattgtc atgagtcttt ctattgcgat cagcagtttt
     4441 tattttggtg ttggaccatg ggtcatcggc caatggatca gctatactgt tgttctgagt
     4501 ttgttttcaa tcgtctgctt gagaaaaaca gtgcaaagga atctatggtt aaagggagtc
     4561 tgtttttttc tggcgggcat ggtttacgga ataggtatga ccgtttttga tacgctgtta
     4621 tatcgtttgc ctcaaccttg gatctattac cttcaagggg tgtcgtttga tttgatgcac
     4681 agtataggca atgttgtgtt ctttcttgtt tttctgccag tcgtcaaacg tttttacaat
     4741 cattcaggag gaaaaaatga aaaaaataat ttatagtttg cttgcaatga gtgtcagtat
     4801 actcattttg acaagttgca gtacaaccta tacacaatcg gcttccaacc aaaaggagga
     4861 agtaaaaaca tcgcaaaaag caacaatcac gttacaagaa aatggtcaaa acttttcgga
     4921 aaaagaagtg gtcttcaaaa aaggcgatga tttactgagt attttgaaac gaaattttga
     4981 agtcaaagaa gataacggct tcatcacttc gcttgaaggg cactcacagg atgagaaaaa
     5041 tcaactctat tggatgttta ctgtggacgg gaaaagtgct actactggtg ccaaagagat
     5101 caaattgaat gatggacaat cggtcatttt caatttgtct aaactataag tttcataaaa
     5161 aactgttaaa gggcgaatat tgccataatt cagtgagttc agttgatgaa agaaggaaaa
     5221 taacatggaa aattttaaaa aaagcggaat gttttccgta attgctgctc taggggctaa
     5281 tattctagtt gctatcagca aatttgtcgg ctatgcaatc agtggcagtg ccgccatgct
     5341 aaacgaaagc atccacagta tcgttgattg cagcaatcag attttgctgt tattcggtga
     5401 taaacaagcc aataaaggac agagcgagtt gcatcaattt ggtgaaggac gagcaaaata
     5461 ctttttcagt acaatcgtcg caatgatgct tttcttcggc ggtggggcac ttggggtgat
     5521 ggaagctttt gaaaagttga cgcatccatc ccatgaagtg ggtaatccga tcattgttat
     5581 agcaatactg ttatttggtt tggtaatcga aggaagttcg ttacgcgtgg caatgaaaga
     5641 aatcaaagaa ttgaatactg aaaaactttc cacgatgcgc tttttgcgcg aaagtcgaca
     5701 cagtgaaata ctgattattt ttacggaaga cttgtgtgct gtaatcggtt tggtcttggc
     5761 tttagcagga acaatattga cttatgcaac cgggattgct gcatttgatg cgatcagcgg
     5821 attgctgatc ggtttcttgc tgatgggagc cgccattttc ctagcaaaag aattttatag
     5881 tctgatcatc ggtgaaagcg tgacagcaag cgatttatca aaaatcaagt tagcttttga
     5941 acgtcctgag atcagtcggc tgatcgatat aaaaacagtt catttaagtc caacggagat
     6001 tttagttgca gcaaaagcag atatcgttgg taacaaagag aatgaagctt actcggtcat
     6061 caatgatatt gaaaaaaata tgcgtcagtc tcttcctgac aagaagatgt atatatacat
     6121 tgaaacagat aaatatgacc caaattattc tcgtaaataa aacgtttatt catgaattga
     6181 agcaaaaatg agaaaaatat aatcttgttt ttaaataaaa cggctacaat ttagcgaatt
     6241 tatgttacaa tgagtccgct gtttatttgt tgtaattaaa ggagatattt taatgaacaa
     6301 aaagaaaagt gcgccatggc ttcaagcttt gcatgcagtg atgccacttt gtataagcta
     6361 tataccagta ggattagctt gcggagtatt gttgcaaaaa gttggatttg atcctctctt
     6421 atcaggactg ttatcgattc tggtcttttc cggtggcgcg cagtttttag ttgcatctat
     6481 gcttacgacg caagcatcat ttgcaacgac attattgatg gttttctttt tagaattacg
     6541 ctatacatta ttaggagcaa gtcttgccgg ttttatgaaa aaagaaaagc ggacgttttt
     6601 ggctgtcttt tctcaatcac tgaatgatga aaactatgcg gtgaactact taaagttttc
     6661 aactgaccct tcctggaata aaagaaaggc tttatatgtc aattggttct caatgactgc
     6721 atgggccggt agcaatatga tcggtaattt tttcggctcg gtgattcgag tagatgctga
     6781 ccttgtccat tttgctttga cggccatgtt cattttcatg tttatcatgc agatgaaaag
     6841 cgcactactg atctttacag gtttattctc aggcgtatta ggggtcgtat tcatggtatt
     6901 attccaaaat actttcggat tgattgcagc aacagtcatt gcttcatttg ccggatttat
     6961 gctggagaag gcattcaaaa aagaaaaagc taagaaacta agagaccgcg gtaaagatgt
     7021 caatgagccg ctcttcaact tcccggaaca ggaggccaat caccatgact aaactatatt
     7081 tgctactttt agttgcttgt ctgttcatcg tggcatatat cccgcgcttg ttcccgatgc
     7141 tttattttac tcatcgaaaa gtgccatcct ggttcagtga ctggatgaaa tatgtaccgg
     7201 tggcattatt tgcggcgtta gcttttaaag atgttttcat tactcatgaa catctggata
     7261 ttgcttggaa tatcaaaata gctgcaatga ttctagtagc aggcgtggct tacaaaacac
     7321 gttcgatggc gttgtcagtt ttgacaggac ttgcgtctgt ctttttgctg tctatgcttt
     7381 aaactgtttt attttattaa aaacagagat tgcgacataa gttgttcaaa ctgatctatc
     7441 cgaactgcat ttgcaacagt caattctgga tgcaagttta ttcgtaggga taaaaaagat
     7501 tagtgttggc ttatgatgca agctcttttt tattttagga attagagagg gatttgtcct
     7561 tctatgattt tttgctataa tagggaagat gtttatttta tgaaagaata gttgagggaa
     7621 tttaaatgaa taaaaaattg atgcaaggaa cattttggct aacctttgct aatcttctat
     7681 gtaaagtttt aggggtagtc tatttgattc cctggctaag tatgatgggg aacaatcaag
     7741 atggtatgct tgctacgaca ttgtataatg ttggctatct tccttatgga ttgttcctga
     7801 tgcttggtac agtcggattt ccaaatgcga ttgcaaaaaa agtagctgta gccacaaaag
     7861 aaggagataa cgctgcttgt cggctgattt ttagaagtac gatcaatatc atgtttgtca
     7921 tcggtatcgc ctctgctgca ctgatgtatt tatttgctcc tttactagcc ggaatcagtc
     7981 cgattgctaa cgttaataat gggatactag ctatcagaag tttatgccca tctttgattg
     8041 ctatcccaat tcttagtgca atgagaggat atttccaagg aaaaaatgat ctgcggcctt
     8101 atggaacttc tttgattatc gaacaggctg tgcgagtgat cgttatcttg gctggtactt
     8161 attacttgcg cgtattgact gacggaacca tattagaagc tgtattgatc agtacagtcg
     8221 cttctttctt cggtgggtta gctgcgatta tccatatgta tatcgtcgga cgcagaaaag
     8281 acttcttcga acttaaagat tttttgattt ccagccgtta tttcgaaaga gaaaaccgat
     8341 ctgcctctgt ctctatcatt cgggaaacac taccgtttat ttttgtcggc tcagtcatta
     8401 ctatcgttca aatgatcgac caagtaacga tgaaaccact gcttcacttt ttccgtccag
     8461 agatcgcaga tcagcaactg gaattcttat tcagccgtgc ttctgtcaat ccaaataaat
     8521 tgacattgat cctgatctcc atggtaggaa cagtagctat aagcagcttg ccaatattaa
     8581 gcacgatgaa gaaaaaagat cggatacaaa tcgaaaaaac agtaggagat agtttttcga
     8641 ttgcgttatt gattcttctt ccttcattga ctggaatgtc tttattagct ggaccactat
     8701 acacattgtt ttttggctat gatcctgaaa gtgtcggata tttccaaatg gctttgctag
     8761 cttctttgtt tttctcttta ttcacgatcc tatctacgat gcttcaatct ttgaatcacc
     8821 atcgttttgc gatccgatta actgtagaag caattatcct gaaagtcatc ttccaagtaa
     8881 tcggattagg attagttggc ggatacggga tgagcttatc aaatggctta gcattcggta
     8941 ttgtctttgt tcgcggctat cactttatgt gcaaagaata ccgaatagca cctttagcaa
     9001 agatcagcaa ttttttctta aaaactttcc gctccaccct gattatgctt gcagtatgtt
     9061 ttgccgtttt ctttttgctg aatcagcgat tgtcaatgga atcaaaaagc tatgcaatga
     9121 tttattgtgt ggctgtagga agtattggcg gcttagtatt tttattttct caattcggaa
     9181 aaaatgggtt tcgaatgttg aaaaatgtta aacatcatag cagaagcaaa gaataggtta
     9241 actaatcgtt ttttgctgtc acttgaaaaa cggcaactcg tatgattacg cttgtcgttt
     9301 ttctattttc caattttagt ttcatatcta atcttactta tgatatagtt ataaggtctg
     9361 gaaaaattct ttgaaacgag gaaattaaac acatgaaact tgcagtagtt acagatagtt
     9421 cggcatatct gccggaacgt atcaagaacc atcccgactt attcgttatt ccaatccctt
     9481 tgattatgga tgggaaaatt tataacgaag gaatcgatat cgaagcggat gaatattatg
     9541 gacttttaaa aaacagtaaa gattttccta ctacttcaca acctgtcgta ggggaagttt
     9601 tgggtttata tgaggaattg aaagagaaag ggtatgacac gattttaagt atccatcttt
     9661 cttcagggat ttctggcttt gtcaacacat tatttgcgat gaaagatgac atcagcggag
     9721 taacggtcat tccttatgac tctaagatca ctagtatgcc gatgggacac atggtagaag
     9781 ctgccttaga cttaaatgat gaaggtcagt cccttgatga aatacttgca catatcgatc
     9841 gaatccgaga taatacctat gcttatctga ttgtagatga tttgaacaat ttagtacgag
     9901 gaggacgttt gacaaatggt gcagcgctga ttggcagctt attaaaaatc aaaccgatcc
     9961 tgacattttc tgaagggaaa attgttttat tcgaaaaaat ccgttcaagt aaaaaagcat
    10021 ttgctcgagc tgaagaaatt attggtagaa gaaataacga aatacaaccg ccagtcaaac
    10081 tttacgtgat acatgcaaat aatttagaag tagcgattgc ggagaaagag aaattgcaga
    10141 aaaaatatcc aaatgcagaa atcgaaattg gtcatttcgg accagtcatt ggtacacatt
    10201 taggtgaaaa agccatcggc attgccattt cagcgcaata attcctttta ttcaaaaaac
    10261 ataccaaaaa tat
//
LOCUS       DANKEF010000079        10132 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.079, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000079 DANKEF010000000
VERSION     DANKEF010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10132
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.079"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(115..360)
                     /locus_tag="PWZ48_002456"
     CDS             complement(115..360)
                     /locus_tag="PWZ48_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262092.1"
                     /translation="MVDKSKKRQYLLVPLSIILSALEGDPISIKMILKHYHSYMAKLC
                     LTNGFNEVGQFVTYVDEYMLSQLEIKLIEAILKFKIN"
     gene            complement(354..755)
                     /locus_tag="PWZ48_002457"
     CDS             complement(354..755)
                     /locus_tag="PWZ48_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2262093.1"
                     /translation="MQQHYNDISIQHQFDAYCKKVLRNEAKSIRKRNANIKDNEEPLA
                     DSNEGRYSQTHFDEQDVYFLFGMEILISDQKLSAAIDQLSEIRRKIILLYYFAGFNDA
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            complement(942..2750)
                     /gene="uidA"
                     /locus_tag="PWZ48_002458"
     CDS             complement(942..2750)
                     /gene="uidA"
                     /locus_tag="PWZ48_002458"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDL2262094.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPINEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVTLPKRLLEERLVLRFGSATHEAKVFLNG
                     KFITQHKGGFTPFESEINRFVKDGNNRITVAVNNIIDDSTLPVGIMKETVQENGDIKK
                     ENLVNFDFFNYAGIHRPVKLYTTPKDYISDITINTRITDLDASVEFNIDTEWTGKILI
                     AIYDEEAQLVAEAEGKKGMIQLKNPIFWEPLDAYLYSMKISLIDDNKVIDTYTEEFGI
                     RSVQVTNGKFLINNKPFYFKGFGKHEDSYVNGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHNTWKEIRTENSHKT
                     VIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDVCKVGDIVDVLCLNRYYGWYVSGGDLAHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGFHDTVPTMFTEEYQVAYYEANHEVIDTCENFVGEQVWNFADFATSQGILR
                     VQGNKKGIFTRDRKPKMIAYYLQKRWSSIPNFSYKK"
     gene            complement(2773..4539)
                     /locus_tag="PWZ48_002459"
     CDS             complement(2773..4539)
                     /locus_tag="PWZ48_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HDL2262095.1"
                     /translation="MTIKVDLRKKPYNLDEDAISWIEETIDSMTLEEKIGQLFVNMGS
                     SRDKNYLSNMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQNPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLGTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNPDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYRKG
                     IITDDRLLDALKRILGLKAKLGLHKKAKEQILLPKQEALEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKNLTKKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGVSPIDAYCGLKDTFI"
     gene            complement(4552..5346)
                     /locus_tag="PWZ48_002460"
     CDS             complement(4552..5346)
                     /locus_tag="PWZ48_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HDL2262096.1"
                     /translation="MNNWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVTSINRLKHWVSLTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNQQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAVQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDVLFKCLSGDYVDIEQKYIDTFWKNLTNK"
     gene            complement(5356..6732)
                     /locus_tag="PWZ48_002461"
     CDS             complement(5356..6732)
                     /locus_tag="PWZ48_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2262097.1"
                     /translation="MERKFGIRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     AVGTLFLVARFIDAVTDVTMGRIVDRSKATKQGKFKPWILRMSGPVALASFLMYQTSM
                     ISASMSVKVVYMYVTYILWGSIFYTSINIPYGSMASAITSITEERTMLSTFRNVGATL
                     ASLVIGVLTPLFIYTRDDSGAQIVRGGSTFTIVAGIFSICALICYLICYKLTTERVRI
                     DQSQYKVSLLKSLKGLVKNRALLSIIGAAIFLLLAQLLIMSMNNYVFPDYYNDAQGIV
                     LMNFINPILVLIVVSPLTLFLSKKYGKKELASVGMFFSAIVYLILFLIKASNMYVFLI
                     LSSVGYMGLGVFNTVIWANITDVIDDQEVKSDQREDGTVYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLANVQTGDVLEKLYNASTLIPSICFLIVGLILFFLYPLSKDKVLENC
                     ELLKQKHK"
     gene            complement(6852..7499)
                     /locus_tag="PWZ48_002462"
     CDS             complement(6852..7499)
                     /locus_tag="PWZ48_002462"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-keto-4-hydroxyglutarate
                     aldolase/2-keto-3-deoxy-6-phosphogluconate aldolase"
                     /protein_id="HDL2262098.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINSIIAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVDIIKLFPGSAFGPSIVKAYQAPLPHVNIMP
                     TGGVSIENLEDWFEVGVVAVGVGGNLLASVEKGDFEEVSRVASKYIDKFYEIKNY"
     gene            complement(7583..9010)
                     /gene="uxaC"
                     /locus_tag="PWZ48_002463"
     CDS             complement(7583..9010)
                     /gene="uxaC"
                     /locus_tag="PWZ48_002463"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDL2262099.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAKHQPIIDYHCHLDPAYLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGPGDDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLEMMDVKAICTTDD
                     PTDDLWYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQTAAIPIKDFNGL
                     KQALSQRVDHFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIGSRK"
     gene            complement(9084..>10132)
                     /gene="uxuA"
                     /locus_tag="PWZ48_002464"
     CDS             complement(9084..>10132)
                     /gene="uxuA"
                     /locus_tag="PWZ48_002464"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDL2262100.1"
                     /translation="EEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPRERIKSLKDEVE
                     ANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVICYNFMPVFDWV
                     KSDLDYRLDDGSSTLAYISKNIPTDPEEIIREVENSTNNFELPGWEPHRLSQIKQLLE
                     AYKEVDEEKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLPRIVKNRDDLDW
                     LCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRNIKYLSDNGLDF
                     YEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGYGLYDRALGAVY
                     LNGIWEALEKGASK"
BASE COUNT         3243 a         2036 c         1294 g         3559 t
ORIGIN      
        1 agaatctatt taattttcaa tgaacaatag gttttctacc taaaagaatc tactaattcc
       61 ttaactatta gaagattctt ttaaacagaa aaagtggaaa ggggactttt ttagttagtt
      121 aattttaaat tttaagatcg cttctattaa tttaatttct agctgactta gcatatattc
      181 atctacatag gtaacgaatt gacctacctc gttaaatcca tttgtcaaac ataacttagc
      241 catataagaa tgataatgtt tgagtatcat cttgatcgaa attggatctc cttctagagc
      301 agataagata atggatagtg gaactaatag gtactgccgc tttttagatt tatctaccat
      361 aggtcatacc ccattttcaa ttgttcaact gccttctttc gttgatacca gataccactt
      421 gtgctcatat tgagaatttt accgatttca gcatcattaa aacctgcaaa ataataaagt
      481 aggataatct ttcttcttat ctccgataac tgatcaattg ctgctgataa cttttgatct
      541 gaaattaata tttccattcc aaataagaaa tagacatctt gttcatcaaa atgagtttgg
      601 gaatacctgc cttcatttga atctgccaat ggttcctcat tatcctttat gttagcgttt
      661 cgttttcgaa tagacttagc ttcgtttcgt aatacttttt tacagtaggc atcaaattgg
      721 tgttgtatag atatgtcatt gtaatgttgt tgcatgataa tatgctcctt tttccccctt
      781 tccactaata caacaatcag aaagcccctt tttttcgaaa aaactagaaa aaaaacataa
      841 aaatatttat cattctttaa acagaaaatc tttctataaa aaacaaaaaa aaacgattag
      901 gctaggctaa tcgttttttg aacattctta aaaaatccaa attatttttt ataactgaaa
      961 ttgggtattg atgaccatcg tttctgtaga tagtaagcaa tcattttagg ttttctatct
     1021 ctagtaaaga ttcctttctt atttccttgc actcttaaaa tcccttgact tgttgcaaaa
     1081 tctgcaaaat tccacacttg ttcaccaaca aaattttcac atgtatctat tacttcgtga
     1141 tttgcttcgt aataagccac ttgatactct tctgtaaaca ttgttggtac tgtatcatga
     1201 aatccagcta ctgtatctgc tccatattct gtaaacatta ttggcgtatc aggacatctt
     1261 tccatccatc catctagctc ttttttcaac agatattttg catgtgccag atcccctccc
     1321 gaaacatacc aaccataata acgatttaaa cataaaacat cgacaatatc tcctacctta
     1381 caaacatctg gcgttgattt tagataagta acgattgtta caggtctttt ctgcggatca
     1441 agttctttag ttaattcaac taaaggttca aaatattctt tagcaccttc ggaatccgaa
     1501 tctggttcat ttgcaataga ccacattaca acacttgggt ggttcttgtc gcgttctatc
     1561 aattccttga tcaccgtttt atgagaattt tctgttcgta tctctttcca cgtattatgt
     1621 ttttccacat tatcatctaa taaagtggcc atgaaattta aatgtaaccc tactgcaggt
     1681 acttcatcaa taactacaat accttctcga tctgctaatc gcataatttc ttctgagtaa
     1741 ggataatgtg atgttctaaa agaattagca cctatccatt tcattaaatt aaaatcttta
     1801 atatttacag cctcattcaa accacgtcca ttaacatacg aatcctcatg cttaccaaat
     1861 cctttaaaat aaaaaggttt attattaatc aaaaatttgc cattggtaac ctgaacactg
     1921 cgaataccaa attcttctgt ataggtatcg atcactttat tatcatcaat taaactaatt
     1981 ttcatactat ataaataagc gtctagaggt tcccaaaaaa ttggattctt aagctgaatc
     2041 attcctttct ttccttcagc ttcagctaca agttgagcct cttcgtcata tatcgctatt
     2101 aagatttttc cggtccattc tgtatcgata ttgaattcta cacttgcatc caaatcagtt
     2161 attcttgtat taatagtaat atcacttata tagtctttcg gagttgtata taacttaaca
     2221 ggtctatgaa tacctgcata attgaagaaa tcaaaattaa ctaagttttc cttttttata
     2281 tccccatttt cctgtactgt ttccttcata atccccacag gtaaagtact atcgtcaata
     2341 atattgttta cagcaactgt aattcgatta ttcccgtctt ttacaaaccg attaatttca
     2401 gactcaaatg gtgtaaaccc acctttgtgt tgagtaataa actttccatt caaaaaaact
     2461 tttgcttcat gtgttgcaga tccaaaacga agaactaatc tttcttctaa taatctttta
     2521 ggaagagtca cttctttttc ataccacacc catccaacat gatttctaat gttctctgtt
     2581 tcaaataaat cattataaga agagggaact gaaatcggat aactctcttt ttcatttatt
     2641 ggttctaaag caagttcttc ctgtaagtca tcatttgtat ctaatttaaa tctccagaga
     2701 ccgcttaagt caattacttg cctcgtctct gtcataatgg gatacaacat ataaaccact
     2761 cctatctaaa tattaaataa atgtatcctt taaaccacaa taagcatcta ttggacttac
     2821 tcccttaaat tcagaaagac ccattagttt gtccaccaat atttctaaag tttcttgttt
     2881 tccatcataa gtattaatat aagtctttac ttgaggtaca tcagccaaat gaaacggaca
     2941 ctgtacagat acaaaaattg ttggaatttc atgaatgtaa aacggtatat ccggagttcc
     3001 tttacttgct ggccagacaa ttcgttgaac tgtgcctgga ttaacattag ctatattaat
     3061 gatcaagtca tattttttag ttaaattttt aattggtctc ttagcagcat atattccagc
     3121 tatattttta attttttctt cttgcgataa tcctttcaat tcttcttcag gtgaaatgta
     3181 tttttcaact tcaaatcctt ttgctgacaa aatctgtttc aataaatcaa tggcttgagg
     3241 tctatttcca gcaactactt caccaaaccc accttttacc ccatgtacat ctactagtaa
     3301 aatcctttta tatttttctg gtacaatagg ccaaatatct tgtttatctt taactaatgt
     3361 aattgcttgg tcagccacct ctttaaaaat tgatttattt tcaattaaac caattttttc
     3421 cagagcttct tgcttaggta ataaaatttg ttcttttgct tttttgtgta atcccaattt
     3481 agcttttaac cctaaaattc tttttaaggc atctaataac cgatcatcag tgataattcc
     3541 tttacgatag ccttccatca tataattaaa gtcttcatct ggatcattaa aaaatagaaa
     3601 tagatcacat ccagctgcaa tagaagtggg caataattct gatcgtttca ttgccccagt
     3661 cattcctaac atatgactag catcagtaac tactaatcca ttaaatccga gttttcctct
     3721 aagtaagtca gtgattaatt cttttgataa agttgcagga agtaaatctt catctttcag
     3781 atcaggatta aaatgttttg aataagctgg catatggata tgacctgcca taattgaagg
     3841 taatccagct tctattaagc ctgagtatac ttttccaaaa gaattatccc attcttccgt
     3901 acctaaacta ttaatactaa aagatagatg ctgatctcgt tcgtcaatac cgtcaccagg
     3961 gaaatgttta gctgcaggca aaattccact ctcctgaata cctttcatat aagctaaact
     4021 aagttctaaa actttatcag ggttttgtcc aaaagatcga ctagaaatga taggatttct
     4081 ccaatttcta ttaatatcta caataggagc aaaactccag ttacatccaa tagccgatgc
     4141 ttcaatccca gaaactctcc ctaattcata agcatatttc gcatcatttg ttgcaccaat
     4201 ttttacttct acaccaactt cagtaccatc tgtacaagct ccgttaccac ctgcttccgt
     4261 attagcagca attaatagag gaattttact ttctgtttgt aagatatagt tttgatcata
     4321 tacctcatca gcagttcctg gattatatcg tacagctcca atatggtact gattgaccat
     4381 attactcaag taatttttat cacgactaga tcccatatta acaaacaatt gacctatttt
     4441 ttcttccaaa gtcatagaat caatcgtctc ttcaatccaa gaaatagcat cttcatctaa
     4501 attatatggt ttttttcgta aatctacttt tatagtcatt ttctttctcc tctacttatt
     4561 agtaagattt ttccaaaaag tatctatata tttttgttca atatctacat aatctcccga
     4621 taaacattta aataaaacat cagttcttaa gttttcccat gattcttttt ctttattgac
     4681 caaaatagga taaaagaaaa ctttattttt atttgcagct tctagatcac caggagaatc
     4741 accaatcatt aatatcttat ccaaatcaca accattttca attacacttc gaataatatc
     4801 ttcttttttt cctttatctt gacaacacag ttcatcaact aatgataata attcatgtct
     4861 ttcccattct tcttgaacag cttccttatt agctgagctt acaataatta tcttcccata
     4921 ttttttcaga gcttttatag tatctctagc acttaaaaag ggtttatcta atccttttgc
     4981 ttgactaatc tgttgattga cttcttccga ccattttaaa gcttttaata aatcctctga
     5041 ctgagaatat tctatttccc tcttcaaaga tggattagac aattcttttg ttagagatac
     5101 ccagtgcttt aatcgattaa tcgatgttac atcatttttt tctaatgttt taactaaacc
     5161 taaaaatctg tttattccac gagtctctga atataaatta atattattcc aatcctgtag
     5221 gaatttttct ctatgatgaa catcaaaaat ctctgcagca attggtccga aaaagagatt
     5281 gtgtttataa gtcatcgtat ccataacaca gccatctgaa tcaatacaaa atatccaatt
     5341 attcatattt ctctcctatt tatgtttttg ttttaaaagc tcgcaatttt ctaagacttt
     5401 atctttagat aatggataaa ggaaaaataa aattaatcct acaatcagaa aacaaattga
     5461 tggaattaat gttgaagcgt tatataactt ttctaaaaca tctcccgttt gtacatttgc
     5521 taaagagtca tagcctataa tagaaagagt ccaacctccg acgcctccag ctagagcttg
     5581 acctattttt ctagcaaaag aatataccgc atatacggtc ccatcttctc tttgatcaga
     5641 ctttacttct tgatcatcaa taacatcagt aatatttgcc caaataacag tattaaacac
     5701 acccaaaccc atatagccaa ccgaactaag aattaagaat acatacatat tactagcttt
     5761 tatcaaaaat aagatcaaat atactattgc tgaaaagaac attcctactg atgccagttc
     5821 tttttttcca tattttttac ttaaaaacaa agttaaaggc gaaacaacta ttaaaaccaa
     5881 aataggattg ataaaattca ttaaaactat tccctgtgca tcattatagt aatcaggaaa
     5941 tacataatta ttcatactca taattaataa ttgagcaagg agtaagaaaa tagccgcacc
     6001 aattatagaa agtaatgccc tatttttaac taaccctttt aaagatttta acaatgaaac
     6061 tttgtattga gattggtcaa ttcttactcg ttcagtagtt agtttataac atattaaata
     6121 acaaataagt gcacatatcg aaaatattcc cgcaacaatt gtaaaagttg atccaccacg
     6181 aactatttga gcgcctgaat catctcttgt ataaataaat aatggtgtta atacaccaat
     6241 gaccaaactt gctaaagtag ctccaacatt tcggaatgtc gatagcattg ttctttcttc
     6301 tgtaatagaa gtaatagctg atgccattga tccataagga atatttatag atgtgtaaaa
     6361 aatactcccc cataatatat acgtaacata catgtatact accttaacgc tcatagaagc
     6421 tgaaatcata gaagtttgat acattaaaaa acttgctaaa gcgactggac cactcattct
     6481 taaaatccac ggtttaaact taccctgctt agttgctttc gatctatcaa caatacgccc
     6541 catagttaca tcagtaactg catcgataaa tcttgctact aaaaataatg ttccaacggc
     6601 agctccgctt atccccaata cttttgtata aaagatcatt aaaaaagaac tagcaaaaat
     6661 gaaagtaaaa tcgtttccaa aatctccaaa catatatcct attttgtctc ttataccaaa
     6721 ttttctttcc atacaattct ctcccttctg ttgttaagcg ttttcattct gatgaaagta
     6781 cgtactatac tctgaagcgt aaagagtcag tatttatttt ttacatttca gagtattatt
     6841 tgttttttct cctaataatt ctttatctca taaaacttat ctatatattt actagctact
     6901 cgacttactt cttcaaaatc acctttttca acagaagcca ataaattccc acctacacca
     6961 actgcaacaa ctcctacttc aaaccaatct tctaagttct ctatacttac tccgccagta
     7021 ggcattatat ttacgtgtgg taatggagct tgataggctt ttacaatgga tggaccaaaa
     7081 gcagaacctg ggaatagttt aatgatatct acgccagcag taagtgcctc ctttatctca
     7141 gtaattgtca tacaacctgg taaataagga atttgatata aattacaaat ctcagctgtt
     7201 tgtcgatcaa aagatggact aacgatatat tctgccccag ccataattgc gatacgagca
     7261 gtaaacgcat ctaaaaccgt accggctccg attattacat ctttttgatc agcatatttt
     7321 ttagcaattt ctttgattat ttcttcagca ttaggaactg taaaagttag ttcaatccct
     7381 ttaattccac ccgcaataat tgaatttatt gctttaatgg cctcatcctt atttttccca
     7441 cgtacaaccg caatgactcc agccgcttct aacttactta aaatttctac ttttttcata
     7501 tctatctcct tcctttatgg atacatactt tgtctagctg tactaactta caaagaataa
     7561 atttaagctt ttatttcaag tattattttc tacttccgat ttcaaaaaaa ttaaaataat
     7621 tatacgcatt acgataacaa atattctcca ctatctgact aagatacatt tcatcatctg
     7681 gaattcggcc tgctttaacc cactcgctta gtaattgaca taatactcga cgaaaatact
     7741 catggcgagg atagcttaga aaacttcgag aatcagtcaa catacctgta aaattcgaca
     7801 acaaactttg ttgtgccatc gtagtcaact gttgacgcat tccttcagct gtatcattga
     7861 accaccaagc acatcccaat tggatttttt gaatcatacc cccttggaag ctttgcattg
     7921 ctgttgctag tggaagccaa tcagttggat ttaatgaata taaaattgtt tttggtaagc
     7981 acccactctt ttcagcggta tcgaacaatg ctacaacatt ttgtgtcaaa ctagcttgat
     8041 cccccatcga atcaccaccg acatctactc caatttgttc ctttaatata cttgaattat
     8101 ttcttaaagc atttaaatgc atttgcatcg tccaaccata tcttgtatac tcagacatca
     8161 aatgtaattg aatacctgta acaaattgtg caatttctga taaatggaaa tccgtttttc
     8221 ctgctaaagc ttttattaac agattgtcga gtattttttc atctgctatt tcataaaaat
     8281 aagtattcaa accgtgatct gctaatcgac agccaatttg atgaaaatga tccactcgtt
     8341 ggctcaatgc ttgcttcaac ccattaaagt ctttgattgg tatagccgct gtttgactaa
     8401 gttttaaaat atagttcgaa aagcctgatg atttgatact ccagacctta tctggacgga
     8461 aagtaggtaa taccttgaaa gaattatcct gtttagaaag ctcagtatga taccacaagt
     8521 catctgtagg atcatccgtt gtacagatgg ctttcacatc catcatctcc aacaattctt
     8581 tcgggcgata ggaatccgtt tgaatttttt cgttagcacg tcgccaaatt tctggtgcat
     8641 tcttctcaga aattgtcaaa tcaatatcaa agtaacgttg taattccaaa tgagaccatt
     8701 caaaaatagg attacctggc gccttctcca ctgtttttac aaaagctaaa aatttttcat
     8761 aatcatcacc tggaccactg atcaactctt cttctatacc attagctcgc atcaatcgcc
     8821 atttataatg atctccagct cctttatcat ttaaccatac ctgagtcaaa ttcttgtatt
     8881 gcttattttc tagcaaatat gctgggtcta aatgacaatg atagtcgata attggttggt
     8941 gttttgcaaa tttatgaaaa agtcttttag ctatttcatt ctctaataaa aagttcttat
     9001 ctaaaaacat atttaaacac tcctttcctt ccgttagttt ttagaagaga aaatttttcc
     9061 tctaaataaa ctcccttata taattattta ctcgccccct tttcaagcgc ttcccaaata
     9121 ccatttaaat aaactgcacc tagagctcgg tcatataatc cataaccagg tctaccggtt
     9181 tctccccaaa tcatccgacc atgatctgga cgaatatagc cattaaatcc attatcgtac
     9241 attgttttta atatcttata catatctaga gaaccatatt ctgatttatg cggcgcttcg
     9301 taaaaatcga gtccattgtc acttaaatat ttgatattac gaacatgtgc aaaaggaatc
     9361 cgatcccgtt gacaaaattc agatagaatt tcatagacat cattatctgg tcgttctgca
     9421 atacttccag tacataatgt aataccatta gaaggagaat ccactacatt acacaaccaa
     9481 tctaaatcgt cacgattttt aacaatacga ggtaatccga aaatggaata tggtggatca
     9541 tctgggtgaa ctgccatttt aatcccaact tcttcacatg tcgggataat tgctttgaga
     9601 aaatatgcaa agttctctcg taatttctct tcatcaactt ctttataggc ttctaataat
     9661 tgcttaattt gtgataaccg atgaggttcc caacccggca attcaaaatt attagtagaa
     9721 ttttctactt ctctaataat ttcctccgga tcagtaggga tattttttga aatataggct
     9781 aacgtagaag acccatcatc taaacgataa tccaaatccg attttaccca atcaaatact
     9841 ggcataaaat tataacagat aacctcaatc ccaaattctg ccaaattgcg aatcgtctgt
     9901 ttataattct caatgtattg atctcgtgtt ggtaaaccaa ttttgatatc atcatgaaca
     9961 ttaacactct caataacttt caaggtaagt ccattggctt cgacttcatc ttttaaagat
    10021 ttaattcgtt ctctcggcca aacctctccg gctggtatat ccattaatgt tccaacaatt
    10081 cctttcattg ctgggatctg acgaatttct tgtagtgaga ttttttcttc at
//
LOCUS       DANKEF010000080        10081 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000080 DANKEF010000000
VERSION     DANKEF010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10081
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.080"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(109..2229)
                     /locus_tag="PWZ48_002465"
     CDS             complement(109..2229)
                     /locus_tag="PWZ48_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2262101.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="PWZ48_002466"
     CDS             2459..3637
                     /locus_tag="PWZ48_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HDL2262102.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="PWZ48_002467"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="PWZ48_002467"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDL2262103.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="PWZ48_002468"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="PWZ48_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HDL2262104.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="PWZ48_002469"
     CDS             complement(4990..6243)
                     /locus_tag="PWZ48_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2262105.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="PWZ48_002470"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="PWZ48_002470"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDL2262106.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="PWZ48_002471"
     CDS             7821..9167
                     /locus_tag="PWZ48_002471"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDL2262107.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="PWZ48_002472"
     CDS             complement(9475..9861)
                     /locus_tag="PWZ48_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2262108.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
     gene            complement(9910..>10081)
                     /locus_tag="PWZ48_002473"
     CDS             complement(9910..>10081)
                     /locus_tag="PWZ48_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HDL2262109.1"
                     /translation="VETVFSSLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQR
                     RFEGTLRYSLGY"
BASE COUNT         3214 a         1839 c         2053 g         2975 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttattata cccgtggtgc
     9901 tagttaattt taatatccta aagaatatct caaagttcct tcaaaacgtc gttgtgctcg
     9961 acttaataga acactgtaag ccagtaatat gctttcaaat ctactctcta gccctttaac
    10021 agaacgtgag ttgaaatttt gacaacctaa tttttctaag gaagaaaaaa cagtctcaac
    10081 t
//
LOCUS       DANKEF010000081         9036 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000081 DANKEF010000000
VERSION     DANKEF010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.081"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..893
                     /gene="menA"
                     /locus_tag="PWZ48_002474"
     CDS             <1..893
                     /gene="menA"
                     /locus_tag="PWZ48_002474"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDL2262110.1"
                     /translation="FAVGVLFSIAFFHEIHWLNTFLFFIGMLVFDLATTAINNYMDYE
                     KAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFVIGVILTINTGWLLLLMGFICC
                     FIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVYVNTFGSDVYDLTVNFSSGQFD
                     LVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRDIENHRYTLVFYIGRPAGTVLF
                     QLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKHLDQFKQTLPQPISFSYSIKNM
                     VLFNASYVLGLLLTIIMQAI"
     gene            complement(1828..3174)
                     /gene="gor"
                     /locus_tag="PWZ48_002475"
     CDS             complement(1828..3174)
                     /gene="gor"
                     /locus_tag="PWZ48_002475"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDL2262111.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(3308..4561)
                     /locus_tag="PWZ48_002476"
     CDS             complement(3308..4561)
                     /locus_tag="PWZ48_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2262112.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(4567..5490)
                     /locus_tag="PWZ48_002477"
     CDS             complement(4567..5490)
                     /locus_tag="PWZ48_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDL2262113.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(5711..8203)
                     /locus_tag="PWZ48_002478"
     CDS             complement(5711..8203)
                     /locus_tag="PWZ48_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HDL2262114.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(8210..8677)
                     /locus_tag="PWZ48_002479"
     CDS             complement(8210..8677)
                     /locus_tag="PWZ48_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HDL2262115.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(8813..8886)
                     /locus_tag="PWZ48_002480"
     tRNA            complement(8813..8886)
                     /locus_tag="PWZ48_002480"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8850..8852),aa:Arg,seq:acg)
     gene            complement(8944..9017)
                     /locus_tag="PWZ48_002481"
     tRNA            complement(8944..9017)
                     /locus_tag="PWZ48_002481"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8981..8983),aa:Pro,seq:tgg)
BASE COUNT         2504 a         1902 c         1505 g         3125 t
ORIGIN      
        1 cttttgcggt aggggtatta ttctctatcg ctttttttca tgagatacat tggctaaaca
       61 cttttttatt ttttattgga atgctagttt ttgatttagc gactaccgct atcaataatt
      121 acatggatta tgaaaaggct cattctgaag tatataagta tgaagaaaat gtcattggcc
      181 gcagaaatct atctcctgta ttgattcgaa acatgatttt agctatgatt gcatttgttt
      241 ttgttatcgg cgttattcta acgatcaata cgggttggct tttacttttg atgggattta
      301 tctgttgttt tattggagta ttctatacat tcggaccgat tcctttatct cgaatgcctt
      361 taggggaaat tttcagtggg gtgactatgg gccttggaat ttttgccatg gtcgtttatg
      421 tcaatacatt cggttcagat gtctatgatc tgacagtcaa cttttcatct ggacaatttg
      481 atttggtagg aaatctttgg gctatagcag caatcgtatt agcttcctta cccttggtgt
      541 ttacgattgc caatatcatg ttggctaata atttgcggga tcttgaccgt gatattgaaa
      601 atcatcgcta tacactagtt ttttatatcg gacgtcctgc gggaacagtc ttattccagc
      661 tattgatgta tgcttgctat ttcgtgattt taatggggtt gttgagcggt atctatgaat
      721 ggccgatctt ggtcaccttc ttgaccttgc caattgtgaa gaagcatttg gatcaattca
      781 agcaaacgtt accccagcct atcagtttca gctattccat caaaaatatg gtcttattca
      841 atgccagtta tgtattgggg ctattactaa caatcattat gcaagccatt taacagcatg
      901 aagaggttgt gaaaaaactg ccctgcatca agtaataaga acagccaaat aaaaatagct
      961 ccttatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg aacaccgttt
     1021 atcaggaggt tgtgaaaaag tcgcttagtt tcgagtatta agaaaaattt tgtgaaaatg
     1081 gctttccaca tttttgcata aatttggctt aataccgaga aactggcttt tgaacaccgt
     1141 ttatcagagg ttgtgaaaaa gtcgcttagt ttcgagtatt aagaaaaatt ttgtgaaaat
     1201 ggctttccac atttttgcat aaatttggct taataccgag aaactggctt ttgaacaccg
     1261 tttatcagag gttgtaaaat cagtgttttg tcctgagtat tagagaaaca aatggaaaaa
     1321 tagttcctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacacta
     1381 tttgttcgga aaaagtgact aactatgaca caattttttt cacagtccct tttttcactt
     1441 gatttgattc gtaccagcga acttgacgag aactttcttg gtttggtaac tatatggtat
     1501 tcttttaaca tcctcgtttg cttcttctaa tttatttata caaagtcaca cttttttaaa
     1561 catctgttct tcgatacatt tctatttctt ttaaacatcg ataccaatat tttgaactta
     1621 agagtgcata ccaaatggat tcatttactc ttcgtcagtt acgaagataa tcaaaatctc
     1681 caacttccct acttatttcg ttttgagaat tatcgaagca atcttacata aataactaaa
     1741 aaacaaaaaa ccagaaaatc agtatactga ttttctggtt ttacatggtt tgttcaataa
     1801 aaagctgttg gaactgttaa tattaaatta tcgcatcgtt acgaattctt ccgctcctgt
     1861 tggatggatg gctactgtat tgtcaaagtc ttctttagtt gcacccatct tgatagccac
     1921 ggcaaaacct tgcagcatct catcgacacc aacaccaata gcatgtaagc cgacgattct
     1981 ttcttctttt ccgacacaga tcaatttcat ttcgcatttt tggcgataat catttaatgc
     2041 aaagtacatt ggggtaaagc gtgaacgata aactttcaat tcattttcgc catatttttc
     2101 caatgcttct tcttctgtca aaccaattgt tgcaattggt ggatgcgtaa agacgactgt
     2161 cggaacaagt ttatagtcaa gataagaatc tttctttcca ttaaataaac gttctgataa
     2221 acgtctacca gctgcgattg caacaggagt cagatctaat ttaccgatca catcaccaac
     2281 agcaaaaata tgttcatccg ttgtattttg gtatttatct actttgataa accctttttt
     2341 gtccaattcc acatttgtat tttccagacc taatttttcg gtattcggta cacgtccacc
     2401 ggcaaaaagt acacaatcac cagttaaggt ttcaccattt tcaaatagca ccgtatattc
     2461 gtcattgttt ttagtaattt ctttagcaat atgatgtgcg taagttttta ttccagcttc
     2521 ttggtacatt tggatcaaat tgtccgaaag cattttatca aacgttctaa gtggtcgttc
     2581 ttttcgatat gcccacatga cttcactacc taaaccacga aatacacctg aaagttcagc
     2641 cgcaatgtat ccagcaccta aaacgattgc tctcttaggt aactctgtta attcaaaaaa
     2701 tccatttgaa tcaattgcgt attctccacc aggaatggtc attttacttg cacggcctcc
     2761 agtagcgatc aagattttag gagcacgata ggtttcaccg tttacttcga tcgtttgact
     2821 atcgataaaa gtagcgtaac cttttatagc ctcgacatga ttgctatcta atcctctttg
     2881 ataagcaccg tgtagaaaat caatatattt ttctcgtctt tctactagac cggcgaaatc
     2941 aacatttttt aaatctgctt ggataccgta acttgaggca tctcgttcaa ttgtttccaa
     3001 aatcgtactt gcttgccaca taactttttt aggtacacaa ccgacgttga cacatgttcc
     3061 gcccaattca ttcgcttcaa tcagcaaaac ttttgcgcca tgcatccctg ctctgtttgc
     3121 tgaagcaata ccgccagagc cgccaccaat tacgatgtaa tcatatattt gcattttgtt
     3181 cctccttatt gaagagatca atcaaaacct ttatttaacg atcctttttc tacagaaaac
     3241 aagtgtaaca cttttcttga attttgtatg cgaagatgct taaaacaggg atatatcagc
     3301 tttttcctta tttacgtttt cggatcatat ctccaggttt caccggctgt gcgactggca
     3361 tagttaaaat catcattgga tttggtgctc ggtcaatgga ttcgccttct tcattatgca
     3421 taacttcaac ggtttgactg aaatgactga atcctggacc ataaaattcg atttcatctc
     3481 ctacagaaaa caagtttctc tgacgtatcg tagcaatctg tgttgcctca tcataagata
     3541 atacttcacc gacaaattta tatactggga ttttacgacg ttcaccgaat aattgttcgt
     3601 tttcagttgg tgtattgtag taaaaacctg tagctaattc tcgttgtgct actttccata
     3661 actcgtcgat ccattcttgc ttgcagacat agttttcagg atcttccata taactgtcta
     3721 cagccgcttt atagacattt gacaccgtag atacataatg gatcgatttc attcttcctt
     3781 cgatcttgaa gctgtctacg ccgttttcaa tcaattctgg aatatgttcg atcattgaca
     3841 tatccactgc actcatcgag aattcttctt ccacttcgcc tgtatcagtt aaactgtttc
     3901 gttcttgacc aaaaggcata tcgaacaatc catacttcca gcgacaagat tgagaacatc
     3961 cgccacggtt tgcatcccgc atcgacatat ggttggacaa tgtgcatcga ccagaataag
     4021 agatacacat ggcaccatgg ataaatgctt cgatttcggt atccgtattt ttacgaatct
     4081 ctgccacttc ctccattgaa acttcacgag ctaaaacgac acgttccaac ccttcttctt
     4141 tccagaattc caatgtctca aaattagttg ctgaagcttg agtggataaa tggatcggca
     4201 gacctggggc ttctgtcgca caaatttcga tcaaagctgg atcagaaaca atcactgcag
     4261 aaataccgac atcacgcaaa ctgcggaaaa attcccctgc tccatcttca tttccttcat
     4321 gggtgaccat attagcagca acgtatacct tcgcatcatg ttcttttgca aaagcaactc
     4381 cttcagccat ttcttcataa gtaaaattac ctgcacggct tcttaaacca tatgcattcc
     4441 cgccaatata tactgcatct gctccataat ggatcgcggt ttttaacttt tccagtgttc
     4501 ctgctggcgc taggatttct ggtcgtttca attttctttt tactgtcatt tctttcatca
     4561 tactccttat ttgatagcag aaggatcaat ataaaagaat cctgtatcta attcacggtt
     4621 ttcaggatgc aatttactga tttcgctagt cattttttcc gctttttctt gatcccagct
     4681 accttctaaa atttgttttt ttgcttcggc gaatagtcga gtgatgttta cgaatttttc
     4741 gcctggagta aataatccgt ccaatttcca tgtacagtaa tgatgctgat aaagttcatt
     4801 caattcattc gagagattca gatcattgct ggcaaaaata tgtgtgccat gactatcttc
     4861 ataaatagaa taatgtgtct cttctttttt aggttccgag ataaaaagac ctctttcacg
     4921 atctttttgt tcgtcttgtt tcgtatagtt atagtaattc tgtagcagtg gacgtttgga
     4981 ttgatggatg catgtagcac catacacaag ggtttctaca ggaatatcga gtttttcttc
     5041 catcgccttc atctcttcaa aaggaacttc acgagcaagt actgctccaa ctgcgccttt
     5101 tttcgcccag aaattgattt gacgtgcgct cgttactaat gtttcagcat cataaatata
     5161 cggtatacgc aattcaggat tttttttcat cgtataaata atacctgtat caccaactgt
     5221 gatcacatct acgtttattt cttttaaaaa agccaaatat tcaggtacat ttttcatttt
     5281 ttcaggatga atgatcccat ttacagcaat attcgctttt ttaccatact tgtgtgctaa
     5341 ttctaccaat tgtcgctgtt cttctcgtgt aaatgaagca ggcagacgca atccaaattg
     5401 ctcttcaccg aaatatagtg tatctgcacc tgcagcaagc aactgttctg cttgttcaat
     5461 agtttctact gtggttatga gttcgatcat aattttctcc tcctacaact tcataatagt
     5521 agcatagctc taaaaacttt ccaatacttc ttctatttaa agtcggattt ttgaaaatta
     5581 tttcacaaaa aatcaaaata aagttcgata tattctgtcc aaatataaaa agagattgtg
     5641 acaaaaatct tgtcacaacc tcttaatcaa gtcttactat tataaaaatt cagctacaca
     5701 ggccaagtaa ctatagggaa cctactgttt cctttttagg tccgcgaaca ttcaatgtga
     5761 tcttaccttt tgaagcacct actgttacgt ggtcacctaa acgaatttct ccgccaagca
     5821 aagcctcgct caatcgatct tcaatttctt tttgaagtgc gcgacggatt ggacgtgcac
     5881 catattcagg atcaaatcca gctttcccga tcacatcgat tgcagatggt gtgattttca
     5941 cttgaatatc ttgttctttc aatcgtttga taatcgcttt gctcatgatt tggacgattg
     6001 tatggatttc atctttactc aatgaatgga agacgacagt ctcatcgatt cgattcaaga
     6061 attcaggtcg gaaagctttc tttaactctt ctaagatacg tttttgcatg gctttgtgat
     6121 ctttagtaac atcttggaca ttgaatccga catttttttc ttctcgaatt tgtgtcgctc
     6181 cgatatttga agtcataatc atgattgtat ttctaaagtc cacttttcta cctttagaat
     6241 ctgttaaatg accatcatcc aatacttgca gaagtaaatt gaaaacgtct ggatgtgctt
     6301 tctccacttc atctaagaga atcactgaat atggtttttg acgaattttt tctgtcaact
     6361 gtccaccttc atcataccct acgtatcctg gaggagaccc gattaatcgg cttgtactgt
     6421 atttttccat aaattctgac atatcaacac ggatcagggc atcttcactt ccaaacatga
     6481 cttctgaaag tgctttggcc aattctgttt ttccgacacc agttggtcca aggaacatga
     6541 atgaaccaat tggacgatca ggatctttca atccgcttcg tgcacggcgg atcgcacgtg
     6601 aaactgcttt gacagcctca tcttgaccaa cgacacgttc atgcagaagt ccttccagtt
     6661 ctaacaagcg ttcgctttct tttttctcca actgctgtag agggacacca gtccactccg
     6721 atacaactgt agcaacatct tcttctgtta cacgatcaga ataaccttgc tcctgttttg
     6781 cttcaattgc agaaactttt tgcagctttt tacgtagtgc tttttcttgt ctgcgtaatt
     6841 gagcagcatt ttcaaagtct tgtttttgga tcgctgcttc cttttcttgt actagttcat
     6901 cgatttctag tttgatcaca gttgattttg ttaagtgatc ggtttgatcc aaacggactt
     6961 tcgctgctga ttcatcgatc aaatcaattg ctttatcggg tagttgacga gaagtgatat
     7021 aacgaacaga aagattgact gctgctcgta cagcttcatc tgtgatctcc actccatggt
     7081 gctcttcata tcttgtacgt aatccttgaa gaatcacttc tgcttcttca ggagttggct
     7141 catctacttg aatgcgagca aaacgtcgct ctaatgcaga atctttttcg atatattttt
     7201 gatattcatc tagggtcgtt gctccgatcg tttgtaattc tcctcgagct aacgccggtt
     7261 tcaagatatt cgaagcatca attgctcctt ctgctccacc tgcaccaatt aaggtatgca
     7321 attcatcaat gaataagatt acttgaccat cattatagat ttcgtcgatg acctttttca
     7381 ttcgatcttc aaattcaccg cgatatttcg taccagcaac caaagatccc atatcaagca
     7441 tcatcaggcg cttttcttgc atgtcttctg gcacttcacc taaaatgatt ttttgagcta
     7501 acccttcagc aatggctgtt ttgcctacac caggttcacc caccagtaca gggttattct
     7561 ttgtccgtcg gctaagaatc tggataagcc gtttcacttc tctcgaacga ccaacaactg
     7621 ggtccagctt gttttctttt gctaatttcg tcaaatcacg tgctaatgaa tctagcgtag
     7681 gtgttccttg cggtgtttgc cgtttatctt gtacatttac atttctacga cgattcattc
     7741 cttttccagc agaatcacca gctagcccca ttttttgttt cagaacttga cgggcttttg
     7801 aaaggctgat acctaagttg atcatgattc gagtagccat gatactttca tcttttaata
     7861 agctcatcaa aatatgttct gttccaactt tttgagaacc agttttcttc acttcgctgc
     7921 tagctaaagc aaaaacttgt ttcattcttg gtgaatatgg aagaaagact cctttagggt
     7981 aagaacggat accaccataa ccaatcagat gttcaatttc ttcataaatt tccgtttcat
     8041 tcaaatccat ttcacgtaat acttttccag caattccatt ttgctcgacc actaaggcta
     8101 gtaaaacatg ttctgaacta actgattgat gtttcaaccg ttttgcttct tcttgtgcaa
     8161 tttctaatac tgcttgggct ctttcagtaa aaagttcttc catctaatct cacttcccat
     8221 cttcataact taaacgttcc agaaatgctc ggagaatccg agcacgtgta tgatcttcat
     8281 attcattgaa attcaatgtg ttttttgcaa ttgcactcaa catcaagttt ccttcttttt
     8341 ttgtaattat ttggtcttca taaagttttt gaataatagc aaaagcattt ttctcactga
     8401 ttgtatcatc aaataactga tttatctgct caagtaattg ttttttatct gaaatcctta
     8461 cttttgcaat tcgtatataa ccaccaccac cacgtttgct ttctactaga taaccacgtt
     8521 ggatggtaaa acgggtatta atcacatagt tgatttgtga aggaacacag ttaaagaggt
     8581 ctgccatttc aatccttcga atctcgatca tttcactatt ttccagaatt ttctttatat
     8641 atgattcaat taaatccgag gtattctgat gtgccatctt attcattccc tttgactaat
     8701 attgacctta attataacga atttgataat agaaagaaag aagaacgctt tttgttaaat
     8761 aaatccttta gaatcaacaa aaaaaaatac ggaatatact attccgtatc gacgcgccca
     8821 agaggagtcg aacccctaac cttttgatcc gtagtcaaac actctatcca gttgagctat
     8881 gggcgcatat tcaatttaaa aaaattataa atcatttgaa aacaaaaagc aagataaaaa
     8941 aaatcgggaa gacaggattc gaacctgcga ccccttggtc ccaaaccaag tgctctacca
     9001 agctgagcta cttcccgttt aaaaaataaa aaagac
//
LOCUS       DANKEF010000082         8814 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000082 DANKEF010000000
VERSION     DANKEF010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8814)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8814)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8814
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.082"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            92..301
                     /locus_tag="PWZ48_002482"
     CDS             92..301
                     /locus_tag="PWZ48_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262116.1"
                     /translation="MNYEEIENRKKVSKEMEEKLLKTMKRKHLKRLSVAQYINDMQLT
                     GKEKACLLGSMKNFEQLRRTYRIHF"
     gene            361..1032
                     /locus_tag="PWZ48_002483"
     CDS             361..1032
                     /locus_tag="PWZ48_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HDL2262117.1"
                     /translation="MKKWLFGFLGVTLIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQKEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGVATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1085..3061
                     /locus_tag="PWZ48_002484"
     CDS             1085..3061
                     /locus_tag="PWZ48_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2262118.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDITQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PTEDSDLTNAKVDEIVKDVEDKGSNSEYVKNATNKITSAVDENGEITFADQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDNTGSGFLKDIHLYPKNIVSKLSFELTK
                     FGDDGTAQSKQTPLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDKEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITRLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKVSV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3076..3828
                     /locus_tag="PWZ48_002485"
     CDS             3076..3828
                     /locus_tag="PWZ48_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HDL2262119.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTIPSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLEPNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            3844..4599
                     /locus_tag="PWZ48_002486"
     CDS             3844..4599
                     /locus_tag="PWZ48_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262120.1"
                     /translation="MRNRKWLFVCAAALFTFTAYTQLTVADSQESTDSTEQTIQTSDS
                     SAIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            4612..4872
                     /locus_tag="PWZ48_002487"
     CDS             4612..4872
                     /locus_tag="PWZ48_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262121.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            4869..6959
                     /locus_tag="PWZ48_002488"
     CDS             4869..6959
                     /locus_tag="PWZ48_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HDL2262122.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            6986..7450
                     /locus_tag="PWZ48_002489"
     CDS             6986..7450
                     /locus_tag="PWZ48_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262123.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            7447..7689
                     /locus_tag="PWZ48_002490"
     CDS             7447..7689
                     /locus_tag="PWZ48_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HDL2262124.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            7707..8009
                     /locus_tag="PWZ48_002491"
     CDS             7707..8009
                     /locus_tag="PWZ48_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262125.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8079..>8814
                     /locus_tag="PWZ48_002492"
     CDS             8079..>8814
                     /locus_tag="PWZ48_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262126.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNE"
BASE COUNT         3256 a         1359 c         1828 g         2371 t
ORIGIN      
        1 aaagaaaaag ataacccaag actgaagtgt agagcgtatg tgatcatagg cttttttatt
       61 tttgcaaaaa acaaggaagg taggaaaact catgaattat gaagaaatcg aaaacagaaa
      121 aaaagtgtcg aaagaaatgg aagaaaagtt actaaaaacg atgaagcgaa aacaccttaa
      181 aagattatct gtcgcgcaat atatcaatga tatgcagtta acaggaaaag agaaagcttg
      241 tttgttaggt tcaatgaaaa actttgaaca gttgagacga acttatagaa ttcattttta
      301 aaactttttc tatggaaatg agaaaaagtt tttttgtttt gtctgaaagg aggcagatgg
      361 atgaaaaaat ggttatttgg ttttttagga gtcactttaa tcgtcttttg cagtgtgttt
      421 ggctatgtta gctaccaaaa acatgaagga gaggtgttta agcaaaatat agaaaaaaag
      481 atgcccgtag atcaaatcaa tgcacatgca aagagttata aggaggatgc aacgaacgtc
      541 aataatgata tgtctttggg gcaaatgttg tccatacaaa aagaagcaat cgaaatgggc
      601 gtcaataaac aagtatttgc acaaattcag attcctgcac ttggtttagc attgccaatt
      661 tttaaagggg caaatcaata cactttgagt cttggagtgg ctacttattt ttatgaagat
      721 gccgaaatgg gcaaaggaaa ctatgtccta gcaggtcata acatggaaat gccaggtgtt
      781 ttattttctg atattcagaa gttgtcatta ggggaggtga tggacttagt tagtaacgat
      841 ggcgtctatc gttacaaggt aacgcgtaaa tttatcgtac ctgagtactt taaattaatc
      901 gatggcgttc ctgaagagaa ctctttcttg tctttgccaa agaaaggaga gaaaccctta
      961 ttaacgttgt ttacttgtgt ctatacgtca caaggaaaag aaagatacgt ggtacaggga
     1021 gaactacaat aacaaggttt taatcaacgc gagtcgttga aatatgaaat gaggagagta
     1081 aaaaatggca ataaatcggt atgtaaaaaa agctggaatg cttattggaa tgttaggaat
     1141 cattgcaggc tgctttcagg gatatcgagc atatgcagaa gatatcacgc aaaaaacgcc
     1201 accagagaag gtcaatatca cggttcataa attaatgtac gatcaaggaa cgcaattgaa
     1261 tgttgatatt gacgggatca aaaacgatgg ttatacgcac gatcaatatc ctacaggtgt
     1321 aacgaaatac aacaaggcag attatggtga tgttgagttt acgttaggaa atattacgga
     1381 tcaagtatta ccgactgaag attctgatct aaccaacgcg aaagtggacg aaatcgttaa
     1441 agatgtagaa gacaagggat ctaacagtga gtacgtcaag aatgcaacaa acaaaattac
     1501 ttcagctgta gatgaaaatg gtgagattac ttttgctgat caaccagcct atgtcaattc
     1561 caaagggaat gtatatgtgg tttatgaaag taaatccgct gctggattag tcacacaaaa
     1621 agcaaaacca atggttgtga ttgcgccaat gactgataat acaggttcag gttttttaaa
     1681 agacattcac ttatatccta aaaatattgt ttctaagttg agttttgaat taacgaaatt
     1741 tggtgacgat ggcacagctc aaagcaaaca aacaccttta aaaggggcaa aatttgagtt
     1801 atacaagggc gaacctggaa aaggaacaaa attaggagat cttgtatcgg atgatcaagg
     1861 aaagctgact gctactgatt tgacgttagg aaaatactat tttgtggaag ttccttcaga
     1921 agttgtagtg ggatcagata aagaaccaac agcagatcaa tatttattag gcgcagacgc
     1981 tcgcaacgat gcgcacaata aattaacctt tgaaattacg aatgatggcg tgacaagtga
     2041 cttaaaagct tcatacgtaa actataaagc accagtgatc gataaaacag taacaaatgg
     2101 aacaggacaa gaacattctt tccaaattgg tgacgcagta aattatcaag gaacgattca
     2161 tatcccaaca gacattgcag gcggagcaga cggtatcaca gtaaacggag tgaaaagcga
     2221 aacttctcct tacagtgtat ttaaatgggg ggatacagca ggacaaggac tttcttatgt
     2281 cgctgctaaa gcaaatatca aagtaacaaa taaagatgga tctgtggtat taaaagaaaa
     2341 tacagattat aaaatccaaa attcagaaaa tggctttgtg atcgatttta tcgtgaataa
     2401 tggccaagta agtgatactg tagcaagttt acacggacaa gatcttcaaa tgacttataa
     2461 catgtatgta aatgacagcg cagctgttgc caatccatta acaaatagcg tggatttcgt
     2521 ttataacaac aatccattta atcaggaaga acaccacgaa aaaacgaaag ctgacgttgt
     2581 gacttatggt gcaaaattct taaaagtcga tagcggacta tttggaacag gaatcaaagc
     2641 aacaccgctt gaaggcgcag aatttgctgc taaaaacgca gaaggaaaat attatggtgg
     2701 attagttgat acggataaag acggtgtcaa agaagcagta tgggtagacg atgtagcaaa
     2761 tgcagcgatc ctaaaatcag ataaagaagg tcactttgaa atcactcgtt taactgaagg
     2821 tgaatattca ttggaagaaa caaaagcgcc tgaaaactat caaaaattaa cgaaagagat
     2881 ctctttcaaa gtagacaaag attcatacaa agaagaaaac cgaatcacga tcaaaaacaa
     2941 tcaaaaagtt tccgtaccaa tgactggtag caacggtttt caaacgtatg tattaatctc
     3001 ttgcttgttg ctaggagcag gggcactttc tgcagttgtt tactttaaga agaaagccta
     3061 gaaggatcaa aaccaatgat tatcaggaag cctaagaaaa agcgaatcat gggtaaatgg
     3121 atcattgctt tctggttact ttcagctgta ggtgtcttac ttttgatgcc tgctgaagcg
     3181 agcgtggcga aataccaaca aaatcagcag attgccgcaa tcgatcgaac aggaacggca
     3241 gcagaaacgg attcttcttt ggatgttgct aagattgagt taggcgatcc tgttggtatt
     3301 cttacaattc caagtatttc tttaaaacta ccaatttatg acggaacgtc cgacaaaatt
     3361 ttagaaaatg gtgtaggtat tacggaagga actggtgata ttacaggagg aaacggtaaa
     3421 aatcctttaa tcgcaggtca tagtggactt tataaagata atctttttga tgatttacct
     3481 tcagtaaaaa agggagaaaa gttttatatc aaagttgatg gtgagcagca cgcttatcaa
     3541 atcgatcgga ttgaggaagt gcaaaaagac gaactacaaa gaaactttgt cacttactta
     3601 gaaccaaatc ccaatgaaga tcgagtaacg ctaatgacat gtacgcctaa ggggatcaac
     3661 acacaccgtt ttctagtgta tgggaagcga gtaaccttta caaaatcaga attaaaagat
     3721 gaagaaaata agaaacaaaa attatcatgg aaatggcttt taggaagtac tgtttttctt
     3781 tcagtcatga tcattggttc gctatttgtt tataaaaaga aaaaataaag gagggtaacc
     3841 aacatgagaa atagaaaatg gctatttgtt tgtgcagccg ctctttttac tttcactgcc
     3901 tacacacagt tgacggttgc agacagtcaa gaaagcacgg atagcacaga acaaacaatc
     3961 caaacgtcag atagcagcgc gattcaaagc agcgatgaaa ccgtcgattc gtcaacagtt
     4021 gattcttcag aagaacctaa aattgctgaa gacgatcaaa tagtcaaaca gaccattcac
     4081 gttcaaaaga ttatttcttc agaacaatta gacaaccagg ggcatgaaaa acaagaagga
     4141 gtaaatggcg cgaagttttc tgtttatgat gtatcggaca ttttacaaaa gatggatgtt
     4201 aaggatctca ctactgacca aattgaatcc caattaaaaa atcgagtgaa aaaactatcc
     4261 agtgatcaat tgaagctagt ttctaatgaa gaaacaaaga caattgatca acaaacaggt
     4321 gtctttgaat tctcggtaga agtacaggca aatcagaaac aagcttatta tattgtcaat
     4381 gaatcaagtc ctgaaaacat ttctaatagc gaggatattt tgctattaac acctgtttct
     4441 gataaaaacg gtgaattctt aaaggatgtg tggatctatc ctaaatcaga agcttcgcaa
     4501 cctaaagaag aagtaaaaaa aatcgtttct actggcgtaa agaaaaactt ctttgagaat
     4561 tgttgggatt ttgttactca cttgttttct agagattaag gaagaaataa aatgaaactg
     4621 atcaaattat tattattcaa tttttcttgt ttaaccgcag tctttttgta ctgcggttta
     4681 ttattttcta aactatctat tttgccaagc taccaactat acctaatgag gattttaaac
     4741 ataccagctg cttccatgcc gctagatatt atggaaaagc tcattaggaa tccattattt
     4801 actacgatca atttgatcgt tattgtcgga atttcactag tcatttataa aaaagagggg
     4861 atgaagggat gaattttaaa cactcgtgga caagacggtt aaaatatgtt tttcccataa
     4921 tgatgatgtt aggtacattg tttgggttaa aaacagccaa tgtctttgct gaaaaagtta
     4981 ttgtcgatcc gtcaaatcca atccagaacg taaagaataa ttttattatt ccaaaagatg
     5041 ttcctaatgc aaaaacaaat attactgtaa acggagcgtc aaaagtttac aaatatcatg
     5101 ttgatgccaa caaaggtggt tttacagacg actacgtagg attagtggag ggaaacaaaa
     5161 atatacgcta tccttctttc caaaaagaat acggtgaaac aaaccttgtt ttagaaggcg
     5221 taacaaccaa caccaaagtc aatattgact atggaaaaat cggtacgtat aacgggaaaa
     5281 aagtcaatat caagctagtt ctatctaata ttcacttata ctcggatacg ttgccgtgga
     5341 atctactaga taacaactat acaaaaactc attttcgaga cgatggctat aaaaatacga
     5401 atggtgcaat gagtaaatct aaaaaaagaa cagtactttg gatctcagac aatcttttct
     5461 caggaattgt ttaccattca acacaaatga atgtgcagct agtagccaca tatgaggacg
     5521 gtagccccgt tcaattttca ggagatacat ttatcagttt taactctttg aaccctgcag
     5581 gaggaaaaag cacggatctt aaaggagaat atgcccacta tgacaaaatg aatacgacag
     5641 attggtatgt cagaaaagat acggttcttt cagaatttaa atctttctac aacaatctga
     5701 acgtggtagg tggccaccca ggaggaagct ctaagctgac acaagcggat aatgatttca
     5761 ataatttgca tgataaatta ggagatccga agtttggtca aggtactgtg tcctttaaaa
     5821 tatctgaagc aaatccgacg tttgtgattg gttcttctaa tgttcaaacc tggtttactt
     5881 tatcttctgc gacaatcttt agtgttgttc ctgatcaacc tgaaaaaaca ggggttgata
     5941 aaaatggaaa taacgtgaac gataaaatgt tgcaagtagg agacacgatt caatatcgaa
     6001 tcaaacagaa ggtgaatcga ttaggagtgg atttactagc ggtttacgat cgatttgagt
     6061 taatcgataa tttaccaaaa gaagtaaatt acgtcgatgc acacgttgaa agtggtacaa
     6121 ataaaaaatt cgatgtatct ggtgaagtga cctatgacaa aacaaaacac caggtaaaat
     6181 atgcagcgaa agctgataca ttaaaaaatc gaatgaagta taacggcgaa acttacgaat
     6241 tagtgatcaa tgtaaaagtc aatgaattgg ccaatcaaaa tagtgtagcc aaaaatcagg
     6301 gaacgtcgat cattaataaa gtagaaaagg atacaaatat cattaccgtt tatttcccta
     6361 aaattccagt gaaagaagta caacaaaatg gaaaagatgt aaacggacga aatgacggta
     6421 agaagggtac gccgacagct cctttaaacg caggatctga agtacaatat ctagtcacac
     6481 aaaaatggca cacaaaaggc gtagacgctg cgtcagatca ctacaaacaa tttagtattc
     6541 aagatccaat tgaagctcgt ttgacatata aagaaggttc tgcgcaagta atagataaga
     6601 gtaaaggaaa agatattact tctgaaggca cactaaccta tgatagcaat tcaagaacgc
     6661 tgaagtggga agcttccgct gacttcttaa gtaacaatct tttagatgga cgagaaattc
     6721 agctgatatt tacagctaaa actccattac aatcagaaaa aaatattgat aaccaagccg
     6781 tagtagcagt agataatgtt tctaataaaa caaatgttgt gacaattggg gttgatccta
     6841 acttgccaca ggtcattgtt cctaaaacag gttctacaca tttagtaacg atttcagcgg
     6901 tcagcttact attacttgtg ttagcaactt ttagttatgc ggttctgaga tacatttaaa
     6961 ataaaaaaac gaaagaatga ataaaatgaa aaagttaata atagtagtta cttccttgat
     7021 tgccatgatt gttattttag tcggatgtag taatgaaaaa aataaacaaa ctaatcaaga
     7081 taatggcgta ttgaaatcag gaactatgtg gaaagaagaa gtaggtggtc ttgtttataa
     7141 tttgaaaatt atagacgaga ctacctggga atactctgag agtgtatggc atccagatcc
     7201 tgtacaaata acagtcaaga ggcaaaaaga ctataaaggt ttagaaagat acaaaatagt
     7261 agattcagct ggtgttaggg aatttataaa caaatctgat tctttattta ttgttgttcc
     7321 ctatgagaag aatggcgtaa aaaaaataat ttttcttgga tctagcaagg atgaaaagcg
     7381 aacgaaagag aaattgattc acgatggatc acaatccaat aaatataagt tacaaaagac
     7441 tagcgagtga ttgctagtct ttttatttgt cctgaaggag gaatcacagt ggcaaaagct
     7501 aaaacgccat ttgaaaaagc cgcgtctaaa atcttagaac gccgcggtgt atctgaaaca
     7561 cagtggaaac aagaagtcct tgaaaaagca caatttgacc tattacgggg aaaagacaag
     7621 gacttagaag gatatatcta tcaacgtgaa cgtgaaaagc ttgttttcga tgaaattatt
     7681 aaacactaaa aggagcgaac aattttatgt ttgaatcaat tacaatgaat tttttaacac
     7741 aaccgaaagt agtagctgcg gttgatattt ttagagcatt acaaactgcc gcaactagtt
     7801 taaaaaacaa tctgatgatt tttgctgctg ctgcggccgt tgcttccatt gttattgggg
     7861 gtcttttctt cctatttgga cgaagaggag ctgaaaatgg gaagatgcac gtaggctatg
     7921 tattgatggg ggtaggagtt attttagcag cagcttctat tgtggtttcg ttatttggat
     7981 tgtttggaca aacacctcaa ggattataag aaagtaaaaa agtaggagta agaagtagtc
     8041 gatacttttt actccttttt tatgaaagga gaatactcat gtactttacg attgcaggca
     8101 ataccaaaac agataaagag ttaataaaaa aagcaacgaa ggaagtagga aagcgtactt
     8161 cttttttacc gaaaacaatt aaatattctg tcattgaaac ggaagaacag ctacatgtgt
     8221 tagggaaagt actggaacac gaaagtcgat cttctgttaa gttgactctc acgttacatg
     8281 acacagaagg aaaaatagag aaagctggta cagaatacgt ttttggccca ctggattatc
     8341 aaatttcaga aggtttctat cctacattga tggatttaat ccgccttgat gtgtttaaag
     8401 aaacggatga aactatgagt gtggaagagt cttatcgaaa cgaaaaactt ttacaagttt
     8461 tagaagaaga gttagagagc gaccaaagaa aagtgaatca agggagtgaa gtcgaagtaa
     8521 atgaagaagt accatgggaa cgaccaatat ttccaaagaa ggagtcaaaa gaaaaagaga
     8581 cagtctatgt tgacgaacaa gctgatattc cgactcagaa atcgatattt gaggaagaga
     8641 acgatgtgat gggtcaattg gaggtagatc cctttgaaga aacaatggag gatgaagaga
     8701 cggccgaagt agaagttgat ccagttgaag cgaatgaaga gtcaacatgg actgaagggg
     8761 attctcagga atatattgaa ccacttatca gtgaagaagt agaaaaaaac gaaa
//
LOCUS       DANKEF010000083         8559 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000083 DANKEF010000000
VERSION     DANKEF010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.083"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            38..126
                     /locus_tag="PWZ48_002493"
     tRNA            38..126
                     /locus_tag="PWZ48_002493"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72..74,aa:Ser,seq:gga)
     gene            141..212
                     /locus_tag="PWZ48_002494"
     tRNA            141..212
                     /locus_tag="PWZ48_002494"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:174..176,aa:Glu,seq:ttc)
     gene            228..300
                     /locus_tag="PWZ48_002495"
     tRNA            228..300
                     /locus_tag="PWZ48_002495"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:261..263,aa:Val,seq:tac)
     gene            310..382
                     /locus_tag="PWZ48_002496"
     tRNA            310..382
                     /locus_tag="PWZ48_002496"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:343..345,aa:Asp,seq:gtc)
     gene            418..493
                     /locus_tag="PWZ48_002497"
     tRNA            418..493
                     /locus_tag="PWZ48_002497"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:451..453,aa:Phe,seq:gaa)
     gene            495..575
                     /locus_tag="PWZ48_002498"
     tRNA            495..575
                     /locus_tag="PWZ48_002498"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:529..531,aa:Tyr,seq:gta)
     gene            582..655
                     /locus_tag="PWZ48_002499"
     tRNA            582..655
                     /locus_tag="PWZ48_002499"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:614..616,aa:Trp,seq:cca)
     gene            658..730
                     /locus_tag="PWZ48_002500"
     tRNA            658..730
                     /locus_tag="PWZ48_002500"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:691..693,aa:His,seq:gtg)
     gene            742..813
                     /locus_tag="PWZ48_002501"
     tRNA            742..813
                     /locus_tag="PWZ48_002501"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Gln,seq:ttg)
     gene            859..929
                     /locus_tag="PWZ48_002502"
     tRNA            859..929
                     /locus_tag="PWZ48_002502"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:891..893,aa:Cys,seq:gca)
     gene            950..1034
                     /locus_tag="PWZ48_002503"
     tRNA            950..1034
                     /locus_tag="PWZ48_002503"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:984..986,aa:Leu,seq:caa)
     gene            1096..1168
                     /locus_tag="PWZ48_002504"
     tRNA            1096..1168
                     /locus_tag="PWZ48_002504"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1129..1131,aa:His,seq:gtg)
     gene            1178..1249
                     /locus_tag="PWZ48_002505"
     tRNA            1178..1249
                     /locus_tag="PWZ48_002505"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1210..1212,aa:Gln,seq:ttg)
     gene            1267..1351
                     /locus_tag="PWZ48_002506"
     tRNA            1267..1351
                     /locus_tag="PWZ48_002506"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1301..1303,aa:Leu,seq:caa)
     gene            1606..2481
                     /locus_tag="PWZ48_002507"
     CDS             1606..2481
                     /locus_tag="PWZ48_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2262127.1"
                     /translation="MKESASCFVLSYSGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAIEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            2585..3349
                     /locus_tag="PWZ48_002508"
     CDS             2585..3349
                     /locus_tag="PWZ48_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HDL2262128.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            3336..4778
                     /locus_tag="PWZ48_002509"
     CDS             3336..4778
                     /locus_tag="PWZ48_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2262129.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAIFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSGFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            4789..6119
                     /locus_tag="PWZ48_002510"
                     /pseudo
     CDS             4789..6119
                     /locus_tag="PWZ48_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
     gene            6132..6590
                     /locus_tag="PWZ48_002511"
     CDS             6132..6590
                     /locus_tag="PWZ48_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2262130.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            6882..8183
                     /locus_tag="PWZ48_002512"
     CDS             6882..8183
                     /locus_tag="PWZ48_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2262131.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEKAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNATLLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPMIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            8210..8470
                     /locus_tag="PWZ48_002513"
     CDS             8210..8470
                     /locus_tag="PWZ48_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDL2262132.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
BASE COUNT         2621 a         1367 c         1858 g         2713 t
ORIGIN      
        1 ctcattttga gaaagagtat tgagcttctt tcactctgga gagttgtccg agaggccgaa
       61 ggagcatgat tggaaatcat gtagatggtg caaactgtct caagggttcg aatcccttac
      121 tctccgtagc aaatttacct ggcccgttgg tcaagcggtt aagacaccgc cctttcacgg
      181 cggtaacacg ggttcgaatc ccgtacgggt cattttaatt gaatgttgga ggtttagctc
      241 agctgggaga gcatctgcct tacaagcaga gggtcagcgg ttcgatcccg ttaacctcca
      301 taatgaactg gttccgtggt gtaggggtta acatgcctgc ctgtcacgca ggagatcgcg
      361 ggttcgattc ccgtcggaac cgtcgttctt gttattcaat taataatttt aaaatatggc
      421 gcggtagctc agttggtaga gcaacggatt gaagctccgt gtgtcggcag ttcgattctg
      481 tcccgcgcca ccatggagga gtagcgaagt ggctaaacgc gacggactgt aaatccgttc
      541 cttagggttc agtggttcga atccactctc ctccatttta taggggcata gtttaaaggt
      601 agaacagcgg tctccaaaac cgttagtgtg ggttcaattc ctgctgcccc tgccatggcg
      661 aatgtggcga agtggttaac gcaccggatt gtggctccgg cattcgtggg ttcgattccc
      721 atcattcgcc ctttttattc ttggggtata gccaagcggt aaggcaaggg actttgactc
      781 cctcatgcgt tggttcgaat ccagctaccc cagtttctat aatttattct ttattttgta
      841 gattgataat agtataatgg cggtatagcc aagtggtaag gcagaggtct gcaaaacctt
      901 catcaccggt tcaaatccgg ttaccgcctt agtactaata aatcaacttg ccggcgtggc
      961 ggaattggca gacgcgctgg actcaaaatc cagtgcccgt tgagggcgtg ccggttcgac
     1021 cccggccgcc ggtattgact aagatgtctt catcttagtt ttttattgag aaattaaata
     1081 acatctttta tgatggcgaa tgtggcgaag tggttaacgc accggattgt ggctccggca
     1141 ttcgtgggtt cgattcccat cattcgccct ttattcttgg ggtatagcca agcggtaagg
     1201 caagggactt tgactccctc atgcgttggt tcgaatccag ctaccccagt tattattttt
     1261 aaatttgccg gcgtggcgga attggcagac gcgctggact caaaatccag tgtccgttga
     1321 ggacgtgccg gttcgacccc ggccgccggt ataattatca atatagcaac tttcttatag
     1381 agagttgcta tatttttttt gtttttatgt aattacctcc aaatcaattc gatacaagta
     1441 ttatcataaa acgagtaaaa aagatattga taacgctttt attatgtgtt aaactatttt
     1501 tgtaaattat gtagggtgct actattcaat taggcacgac ctaagaaagg gttctttctt
     1561 aggtcgtttt ttgtctttaa aaattgtttg gagacaaaaa tatgtatgaa ggagtctgca
     1621 agttgttttg ttttatcata tagtggaggt gaattaacgg tgtatgtgat gaaaaagctg
     1681 aatcataatg ctgtattgat cgatgacgat ggaatagaaa aaattgcgat ggggaaaggg
     1741 attggctttt ctgctaaagt gagtaagcca tttgatgttg aggatgctga caaattattt
     1801 gtattagatt ctaaagaaaa gatcaaacga ttcagtgaga tagtctcgca gattccaatc
     1861 gaatttatgg attttgcgga agaagtgatt gaactgataa cagctaggat ctcctctcca
     1921 ttagattcaa atatctatat tacattgaca gatcatattt attttgctat tcaacgaaaa
     1981 agagaagata gagatgtcac agcaattatg ttgccagaaa tgagattcct ttatccaaat
     2041 gaatatgata tagcaataga agtggtcgag cgaatcaatc atcgatatca aaccgagtta
     2101 gggtcaaatg aagtaggatt catcactatg catatcatta atgctgagct aggagagcct
     2161 aatagtttaa agagcttaaa aatagtagaa atgacgacca cgattttgtc ccatctagaa
     2221 aaatcctatt caaacgttta tgataaagaa tcactgactt atcatcgctt aatggttcat
     2281 atcaaattcc tggttaaacg cttgatttat caggaagaaa cgccagtaga aaatattggt
     2341 ttttttactg aaacatttaa acatagcaaa gcgtattgta cagcactaaa gattaaagat
     2401 atcatcgaac agcaatttca acttgccatt caagaaaatg aaatcatcta tttggcaatt
     2461 catctagcac gaatctatta aaataagggc gtaactatct ataggcgcaa ccaaatgaca
     2521 catagtaaat ggactatgat tatttggttg cgcctatttt agtagaaaaa agaaggagaa
     2581 tgaaatggga aaagtgattt ttaattcgga tgattttggc tatagccatg gtgtgaatta
     2641 tgggattatg gatgcttatc agcgaggaat tttgacatca actacattga tggcaaatat
     2701 gccgggtttc gagcacgctg tgaaactaag aaaggaaatg cctaggctag gtgtaggagt
     2761 ccatttaaca ttgacgtgtg gcaaaccact tctcaaaact gtcgatactc ttatcgaaag
     2821 cggagagttc agatccttat cttattatca gcatccattt aacattgaca aagatcaact
     2881 gtatcaggaa tggaatgctc agattcaaaa aatttatcat gcggggatca taccaactca
     2941 tttagacagt catcatcata ctcatacttt ggatgaaaac caggaggtag ttgttacatt
     3001 agcaaaaaaa tatgatttgc cggttcggag caactttgaa cgaaaagatg aagttcgtca
     3061 tgtcgattat tttgagcctt gtttcgatgt cgtaggagaa actgatgaaa aaaaacgaca
     3121 aatcaatacg agtgtggaag aatatttggc aagtttactc atgatattga gagaagataa
     3181 aacgatcgaa gtcatgtgtc acgcagcgta tttggatcag gaattgttaa atggttcaag
     3241 ttttgtgtat cctcgtactc accaagtcga atttttaatt cattcagcat ttgctcaaaa
     3301 agtgaaaaat gatcctttga ttgagttagt aacatatgag gatatttaat agaggaggaa
     3361 taaaaatgaa tgttaaatca aatgtgcaga agcttggaag agcgatgcta cttccagttg
     3421 cagcgatgcc attagcggga ttgatcatgc gtttatcagc tgatgatatg ctgaacatcc
     3481 cagtaattgg tgcagctgga aatgccgttt ttggaaactt ggatatctta tttgctatag
     3541 gagttgcaat cggttttgtc aaaaataagg acaaaggaat acctgcactt actggttttt
     3601 tagcgattgc taccttgaaa aaaggcttag aaatcatgga cccagaagtg aatatgggga
     3661 tttttggtgg aattatttcc ggtttagtag cggcatggac gtataatcgc tttaaagaac
     3721 aaaaattacc agtagttttt tcttattttg cgggcgaaaa attcccattg actatggtga
     3781 tgattctaca aacaatcgca gctatttttt tgggctttat ctggccgctc atccaaattg
     3841 ggattgataa ttttgcaaaa ctgctcgttg atatgggggc attaggtgtg gggatattta
     3901 tgtttttgaa tcgtttgctg atcccctttg gactgcacca tgtgttgaat acgtatattt
     3961 attacgattt aggaagctat acatctccta gcaaggaagt tttcagagga gagatgacac
     4021 gctttatcaa cggagatcct caggctggac tgtttttatc aggttttttt gtagtaatga
     4081 tgtttggtat accagctata tgtctagcta tctataaagc agcttataaa gaaaataaag
     4141 agatggtcaa aggaatcatg ggaagcggtg caggaacttc atttattgca aatatcactg
     4201 agcctgttga atttagtttc atgtttcttt ctcctatgtt gtacgtgatc catgcacttt
     4261 ttgccggttt agcagggatt gtctgttact tattcaatat acgaattggt tttacattcg
     4321 gagcatgtat tgttgattat ctcatcaatt ttcgaatcgc taccaatgct attttgattc
     4381 ttccaatcgg aattttcttt tttgccttgt attacgttac tttttattat ttgatcaata
     4441 agagaaacat tcagacatta ggacgtgaag ctaaggcaga gtttggcaat gaagtaacct
     4501 tagaagaaac agagttaggg ttagcaagta aaaattatga atatatggca acaaaaatgc
     4561 tacaagcgtt tggaggaaaa gccaatatcc tggatgtcta tagttgtaat acacgcctac
     4621 gggtagaggt agtggatccc accatggtcg aggaacagcg aatcaagcaa ttagggataa
     4681 gcgggatcat taaaccaact gaaaaaaatt atcaaattat catcggctta gaagtgactt
     4741 atgttatggc agaattcaat aaattattag aagaatagaa aggaaaagat gatgatgaaa
     4801 aaacagatta tcactatagc tggaggcggg agtacgtata caccaggaat cgttcaagcc
     4861 attttgaaca atgaaaaacg gttgccatta tctgaaatac gtttatatga tattgatgaa
     4921 gaaagaaata tggatatgta cttgattgtt aaatttatgc tgaaaaaaaa ggtttttcaa
     4981 atattcgcat tcgtgcaacg gatgatccaa agcttgcttt tacaggatgc gattttgttt
     5041 tctcacaaat tcgtgttgga ggattggaga tgcgagaaaa ggacgaaaag attccactta
     5101 aacatggatt agtaggacag gaaacgtgtg gactcggagg atttgcttat gggatgcgct
     5161 caatcaaagg tttattggaa attgtcgatc atatacaaga ctatgccccc aaagcatgga
     5221 ttttgaatta tacaaatcca gagtcgatcg tttctgaagc tgttcgtcgt aaatatccga
     5281 atgtaaaaat gattaatgcc tgtgatatga ctatttcaat tgaagaaacg attgcagtga
     5341 attacggtta tgatcgtaag aattggatcg ttacgtatta tggtttaaac cactttggct
     5401 ggtatacatc gatttatgat aaagaattgc aacgagaaat tatgccagag attattgaaa
     5461 aactaataac aaaagaaatg caagtcgccg attttaatat aggagacaaa acttggcaaa
     5521 aaacctttca aatgatgtca gtcatcacga aaaattttcc aagtaatatc ccgaataatt
     5581 acttagagta ttacttatat cctgatatgg tcgttgagca tactgacaaa gagtatacac
     5641 gtgctaatat ggtcatggat ggacgagaga aaaataccaa agaaatggct gataaaatac
     5701 gccgcggtat tgtggaagaa gtattgaact ttaattttgg ggagcatgga caatatatcg
     5761 tcgatatagc tatttccttg ttgaatgatg atcgtcgtcg ctttatgcta atcgttccta
     5821 atcaaggggc gattccgaat ctacggtcag atgcagttgt tgaaataccg gcatatgttg
     5881 gtgcaactgg tgtggagaca attacactaa gaaaaccaat cagtgatttt cataaaggat
     5941 tgatggaggc acaagttgca gcagagaagt tactagtaga tgcttacttt gaaggttctt
     6001 atcaaaaagc tttacaagca tttacattga atcaaaccgt accaaatgcc agagtcgcaa
     6061 aaaaaatact agatgaaatg atcaaagtaa acaaaaaata ttggccagca ttaaagtaga
     6121 ggtgataaaa agtgcatttc tttaaaacaa gaaacattct aaaagctgta gctagtggtc
     6181 aaattattcc attaacagca gtaaaagacg aggtcttttc ttcaaaaatg ataggagttg
     6241 gatacgctat aacgaatcat gatggatata tccatgcgcc agttaaagga ataatcgata
     6301 atatatttcc tacccgacat gccataacga tcgaaagtga atctggcaga aaaatactga
     6361 tccatatggg tttagataca gttgaactcg aaggaagtcc gtttcagata cgggttgaaa
     6421 gtaaccagga ggtaaaagaa gggacactgt tagctattat agatttgaag aagttggagc
     6481 aagcacaaaa agatacggtg atcattgtag tttctccagg catttttgaa ggaaatttat
     6541 taaaacaaaa ccaagaagta aagattaaag atagttgttt tgaattttag tgtacgatga
     6601 aaaaagttaa atagggtgat atttatgggt gaatttacaa aatttttttg aaatgataag
     6661 tttcaatcac tagatttaat cattttgatt tttgttaatt atcgaatttt tttgttgctc
     6721 ctatgattta gaaaacttat tttcaggatt cttaaaaata aatttgatta tttaagagaa
     6781 tatattgaaa taaaatttct taattatata attagttatg aacattcttt gcgttgtcat
     6841 gcaatgaatg gttcatattt tattgaaaag gagtggtttt agtggaaaag ctgatgaaat
     6901 gggtcgaaga gaaacttgtc ccaccaatgg caaaaattgg tacacagcgt catttattag
     6961 caattcgtaa tggggttgtt tcaacattgt cattgatttt gattgggaca ttttttatgg
     7021 tctttatcaa tttaccgttt cctggatgga atgaatttat tgccccttat tcggcaacga
     7081 ttgttttacc ttttaggatg acgatgggat tgatggctat ttatgcaact tttgttatgg
     7141 gttcggatct agctaaatca tatggattag atagtgtgac aggtggtata ttatctttag
     7201 gaacgttctt tatgttacaa gttcctgtaa acgttttaac tcctgaaaag gcacctcttg
     7261 gctgggtctt acctatgagt tctttgggag catctgggat gtttgcgggg atcttatcta
     7321 tgatttttgc agtggaagta tatcgtttct ttaaaaatcg aaatattact atcaaaatgc
     7381 cagaacaagt gccacctgct gttgcccgta gttttgaggc attgattcca ggtgcagtca
     7441 ttttaacaat tacttggctg attcgaagcg tcgcgggctt tgatgtaaac gctaccttat
     7501 tatcgttatt cgagccttta accaatattt tagggaataa tttgcttggt gttttactac
     7561 caatgttttt gatccatttg ttgtggtcat ttggtatcca tggcatgagt attgtagggg
     7621 ctgttgttcg tccaatgtgg ctgatcatgt tggatgagaa tgcaaaggca ttggcagatg
     7681 gtacagctgc cacccaatta ccctatatcg ctcctgaaca gttttatcaa tggacggtaa
     7741 caattggtgg tgcaggagca acgatcgtgg tatctgtttt gtttatattc ttctgtaaat
     7801 caaaattctt gaaagaggtt ggtcgttttt ctattattcc tggtatcttc aatatcaatg
     7861 agcccatgat ttttggagct cctatgatgt tgaatcctta catgttcatc ccgttcaact
     7921 tagttccttt agttttgact attatcagtt attttgcagt caaattagaa atggtcaata
     7981 gatttactgt tttgccaact tggacattac cagctccaat cggcggttat ctttcagcag
     8041 gaaatgactg gcgtgttgtt atcttgatca tgatcaacac attgatagca tttattatct
     8101 attatccatt tgtaaaagct tatgataaaa agatgctgtc agatgaacaa agtcgtggag
     8161 aaaacgaagt ccaaactgtc taagtttggt ttttagaagg tgggaaacaa tgtattggct
     8221 gattttattt tttgttttta tctttttatt aacggctagt caccttatat tgaatatgct
     8281 agctacatat catattcaaa tcaatcgatg gatttgggca ctggcatcat ttttgatcgt
     8341 gattcttcca aagatcattg taccgcatat gaatgttttg ttttcatggg ggacatatgt
     8401 actttgtgga atttttgcta tcaattttat gatcgagcag catagatggt ttgtcacatc
     8461 gaagctttaa atgttctaaa agtggttgtg acagaagtgg ttagcttcaa gaaataagaa
     8521 gaaatttacg aaaattgctt cttaaatttt tgtgaaatg
//
LOCUS       DANKEF010000084         8490 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000084 DANKEF010000000
VERSION     DANKEF010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.084"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..173)
                     /locus_tag="PWZ48_002514"
     CDS             complement(<1..173)
                     /locus_tag="PWZ48_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2262133.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="PWZ48_002515"
     CDS             complement(593..1714)
                     /locus_tag="PWZ48_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDL2262134.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="PWZ48_002516"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="PWZ48_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HDL2262135.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="PWZ48_002517"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="PWZ48_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HDL2262136.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="PWZ48_002518"
     CDS             complement(4304..5215)
                     /locus_tag="PWZ48_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2262137.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="PWZ48_002519"
     CDS             complement(5232..6239)
                     /locus_tag="PWZ48_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDL2262138.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="PWZ48_002520"
     CDS             complement(6236..8365)
                     /locus_tag="PWZ48_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDL2262139.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8490)
                     /locus_tag="PWZ48_002521"
     CDS             complement(8370..>8490)
                     /locus_tag="PWZ48_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2262140.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DANKEF010000085         8352 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000085 DANKEF010000000
VERSION     DANKEF010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8352
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.085"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(507..848)
                     /locus_tag="PWZ48_002522"
     CDS             complement(507..848)
                     /locus_tag="PWZ48_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDL2262141.1"
                     /translation="MKPNEIVTVYVAFTDKNGGKRRPILVVSDKEDRVEFFGITSQYE
                     NKSDQIKRVYFPIAEWEKVGLKKQSWIDVGSLKAIPKSRENISFKKVGMLSAIDTKKL
                     NRFIERLQRKV"
     gene            complement(838..1119)
                     /locus_tag="PWZ48_002523"
     CDS             complement(838..1119)
                     /locus_tag="PWZ48_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HDL2262142.1"
                     /translation="MKVKNPKEKSRIQVGIDKDLKENAEMILEELGLNPTTAITILYK
                     QVVARGEFPVEIKLSEEEKQGIRLQQLTMDMPVDVLDTDEKLEEWFNEA"
     gene            complement(1226..1810)
                     /locus_tag="PWZ48_002524"
     CDS             complement(1226..1810)
                     /locus_tag="PWZ48_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262143.1"
                     /translation="MNENKMVLSEYARLRTINWLNGMQSSGAFDGFDEENMDFESLSN
                     EDIRYYFIEWYFTEPDEEVEDEPELILERQRILFSEQDSKRLYGLYDLQLKGLKAQYV
                     ADSYDDMVEQGADRVVTYSEDDEEEWENYSDDEICFNDGYMPIEITEEEYDKVSDSDE
                     IGMCTVVSGLDIDVQRVEVAYQKMKERIKSKVES"
     gene            complement(1827..2066)
                     /locus_tag="PWZ48_002525"
     CDS             complement(1827..2066)
                     /locus_tag="PWZ48_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262144.1"
                     /translation="MTKFKSKGMQWCSYYYVGNDEELLRKAKQKGYPDKMLKQIGYYM
                     PEERIEKESCKVLFDSEQLLVLLTQNKLICQFENN"
     gene            complement(2085..2333)
                     /locus_tag="PWZ48_002526"
     CDS             complement(2085..2333)
                     /locus_tag="PWZ48_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262145.1"
                     /translation="MEGMDFIDHEDLIDFGYTWKGMVGISRSLANAFYERNYAVYVLY
                     DDNTESLVDEEYKLDLENVLYGIEKEDLAKYIFSWLGQ"
     gene            complement(2348..2914)
                     /locus_tag="PWZ48_002527"
     CDS             complement(2348..2914)
                     /locus_tag="PWZ48_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262146.1"
                     /translation="MFEKRKDQFPNLEAKSQKVIDGEMIELFQKSYGTIETTASCKYY
                     FQGKRNTFKGFADSDEELAKKIIDWLVSSEHVAREDTVRTTINGEVLTGTRCVCCNHE
                     FVEDEGCYRINEYGDFICQSCHEIEFPTERSWFEYMLQDYEMETENGEVVFCTKEELS
                     KRSDDEIEDWCVWLVGTDDCVAYYTTAE"
     gene            complement(2915..3460)
                     /locus_tag="PWZ48_002528"
     CDS             complement(2915..3460)
                     /locus_tag="PWZ48_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262147.1"
                     /translation="MGERTQALITVKYGNSKTKASVSIHYQWGYGRYMLMDVLNQGIY
                     LINKAYDLRDKSREELAKGIFDRSTGVINFVTYESGVSAYDETGFGGRYEPYQETIED
                     VMKFSDNNDGYAHLEMVFDENGFSLDDKTRLRLFDFEGQQIGLNKFCESGKEFATKDF
                     IKGYRLLLENYGINLEEESVK"
     gene            complement(3486..4253)
                     /locus_tag="PWZ48_002529"
     CDS             complement(3486..4253)
                     /locus_tag="PWZ48_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="JAB domain-containing protein"
                     /protein_id="HDL2262148.1"
                     /translation="MRDRILEVNQEMVNKLTNISGNYEKYDGTLLDNYIFHDTQNIRI
                     GRSKPRAFILIKEKYLNEWNSTYEMVMTDNFEKVLDFRNALLGSDMGNSSLESIEVEN
                     KTENEGEGLIKNVLKIEQKYISEDRSFYRVTSSVGLANFATNLIGNNASESLLVMCLN
                     TKNEVIAYSEVFKGGINQSVAIPREIFQLALLNNSAKIAVSHNHPSGYEFPSENDKSF
                     TTNLKNAGKTIGIELLDHIIVTSKNTSYYSFQENGSL"
     gene            complement(5142..5702)
                     /locus_tag="PWZ48_002530"
     CDS             complement(5142..5702)
                     /locus_tag="PWZ48_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLATT domain-containing protein"
                     /protein_id="HDL2262149.1"
                     /translation="MGDEKKRLEVQLREAYGRVTYTYTTHLKQADNIISDNKILRCLQ
                     IGLSALTTGGVLSTIITDNSELTFVAGLCSTISLGLNLYFKNFDLAEEAKNHQIVADK
                     LWLIREEYVSLLTDLNRLSVEDIVKKRDQLMNETHEAYKNAPKTNSKSYESAQKALKS
                     EEEQFFTEEELDKMLPEHLRKKSSKY"
     gene            complement(5722..6579)
                     /locus_tag="PWZ48_002531"
     CDS             complement(5722..6579)
                     /locus_tag="PWZ48_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2262150.1"
                     /translation="MEEKLIKKDEIIMGLLVPRANTYTIPLTKRQLIAKRYQRITRAI
                     NREFWNSESDTAHSLYVGSYGRGTAISTSDIDIIVELPMAEFDRFKNYLSNGPSKLLQ
                     VIKNAFQEILPNSDIRADGQVVKINFHDGIKFEIVPAFNEKDYWGESKGFIYPDSNMG
                     GNWKATNPKKEQEAMKLKNTKSNNLLYATCKHFRHVRDTEFTSYHLSGIVIDSFVYEA
                     MGNWKFVENNSGGQNISSVSYETALLEYYNSHKVMGGLNLYSPGSNQFVNSDSSIICL
                     EKVLKKIAL"
     gene            complement(6894..7241)
                     /locus_tag="PWZ48_002532"
     CDS             complement(6894..7241)
                     /locus_tag="PWZ48_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262151.1"
                     /translation="MIEYNDRGRLKWSGFYLSDHTERINADNVQRANHNLAKEQMTTE
                     EIAKILRTAVIKNRPVCIQKEERNAEGYYPDDVVGKIIGYDELGVFVEEEKVHYDEIR
                     NVSFSELLKWSQI"
     gene            complement(7234..8172)
                     /locus_tag="PWZ48_002533"
                     /pseudo
     CDS             complement(7234..8172)
                     /locus_tag="PWZ48_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
BASE COUNT         2383 a         1728 c         1105 g         3136 t
ORIGIN      
        1 gctacgcgac cgttcgttcg tcttttcctc cgtgggactc acccaccgca agcggttggt
       61 tgagttgtgg ctcctattca aaattcacta atatgttatt tttgttttct attcaagtgg
      121 gtaacgttcg tttgcaagca aactctctct atgtaacttg ccagttacat cagtggtaac
      181 gctacgtcgc aagcgacttg ctctaatgaa actaccgttt cattctgcct gttcgttcgt
      241 agcaagctac ttcactcact gtatccgtct gaattattgt tatttattac tattcaatat
      301 ccgtcagtca ttttatttat ttacatacat taaatataaa atgtatctta taattcgtat
      361 attactaatt atcactattt aacaatactt aatattttct taatttttat aatcacgtat
      421 atatttacgt gtattttaat atacataaat acgtttatat aaacgtatat cttaatatac
      481 atataaacaa aaaaagaaaa gccttgttag acttttcttt gtaatctctc gataaaccta
      541 tttagctttt tcgtatcaat agctgatagc ataccaacct ttttaaatga tatgttctct
      601 cggcttttgg gaatagcctt taaagaccct acgtcaatcc atgattgctt ctttagacct
      661 actttttccc attctgcaat aggaaaatac actctcttta tttggtctga tttattctca
      721 tattgacttg tgattccaaa aaattctact ctatcttctt tatcactaac caccaaaata
      781 ggtcttcttt taccaccatt tttatctgta aatgcaacat agaccgttac tatctcatta
      841 ggcttcattg aaccactctt ctaacttttc atctgtatct agaacatcaa ctggcatatc
      901 catagttagc tgttgcaatc tgattccttg cttttcctct tcgcttagct ttatctctac
      961 tggaaactca cctctagcga ccacttgctt ataaaggatt gtgattgctg ttgttggatt
     1021 taaccctagc tcttccaata tcatttctgc gttttctttt aaatccttgt ctattccgac
     1081 ttgaattctg cttttttctt ttggattttt taccttcatt tcgttcacct cttttgagta
     1141 taatatactc attattatat ccattaacta catttatgat tatattatac tcttaattag
     1201 ctactttgca aacaatccaa tcaaatcaag attctacttt actttttata cgttctttca
     1261 tcttctggta tgcaacctct actctttgga catctatatc caatccactt acaaccgtac
     1321 acataccaat ctcatcgcta tccgacactt tatcatattc ttcttcggtg atttcaatag
     1381 gcatataccc atcattaaag catatctcat catctgaata gttttcccat tcctcttcat
     1441 catcttctga ataagtgaca accctatctg ccccctgttc taccatatca tcgtaactat
     1501 cagcaacata ctgcgctttt aatcctttca gttgtaagtc atataaacca tacaacctct
     1561 tactatcttg ttcactgaat aaaattcttt gacgttctaa aattaattct ggttcatctt
     1621 ccacctcttc atctggttct gtaaaatacc attcgataaa ataatatcta atatcctcat
     1681 tcgataaact ttcaaagtcc atattctcct catcaaatcc atcaaaagcg ccactacttt
     1741 gcataccatt caaccaatta atggttctca atcttgcgta ttcacttaat accatcttat
     1801 tctcattcat ggttcttcac cccatattaa ttattttcaa attgacaaat caatttattt
     1861 tgagttaaca aaactaatag ctgttcacta tcaaaaagaa ccttacaaga ttctttttct
     1921 attctctctt ctggcatgta atagccaatc tgttttaaca tcttatctgg ataacctttt
     1981 tgtttggctt ttctcaaaag ctcttcatca ttcccaacat aataataaga acaccattgc
     2041 atacctttac ttttgaattt tgtcatgttg ctaccctctt tcgtttattg acccaaccat
     2101 gaaaaaatat attttgctaa atcttctttt tctatcccat ataaaacatt ctctaaatcc
     2161 aatttatact cttcatcaac aaggctttct gtattgtcat cataaaggac atagactgca
     2221 taatttcttt cgtaaaaggc attagcaaga cttctgctaa tacctaccat tcctttccat
     2281 gtataaccaa aatcaatcaa atcttcatga tctataaaat ccattccttc catgctgatt
     2341 cacactccta ttctgctgtt gtgtaatatg caacgcaatc atctgtccct actaaccaca
     2401 cacaccaatc ttcaatttca tcatcgcttc tcttactcaa ttcctctttg gtacaaaaaa
     2461 ctacttcacc attttctgtt tccatctcat aatcttgtag catgtattca aaccaacttc
     2521 tttcggttgg aaattctatc tcgtggcaac tctggcaaat aaaatcacca tattcattga
     2581 ttcggtagca accttcatct tctacaaatt catgattaca acaaacacat ctagtacctg
     2641 ttaggacttc cccattaatc gttgttctaa cggtatcttc tcttgctacg tgttcactgc
     2701 tgacaagcca atcaatgatt ttctttgcca attcttcatc agaatcggca aagcctttaa
     2761 acgtgtttct ctttccttga aagtagtatt tgcaacttgc ggtagtttct atggttccat
     2821 aacttttttg gaataattca atcatttcac catcaatcac tttttgactt ttcgcttcta
     2881 aattagggaa ctggtctttt cttttttcaa acacttattt cacgctctct tcttctaggt
     2941 ttattccata attttctagc aataatctgt atcccttaat aaaatctttc gtagcaaact
     3001 cttttccgct ttcacaaaac ttgtttagtc ctatctgttg accctcaaaa tcaaacaatc
     3061 gcaatctggt tttatcatct agactaaatc cattttcatc gaacaccatc tctaaatgag
     3121 catagccatc attgttatct gaaaacttca tcacatcttc aatcgtttct tgataaggtt
     3181 catagcgccc accaaaccct gtttcatcat aagcactcac tccagattca taggtgacaa
     3241 aattgataac tcctgtactt ctatcaaaga ttccttttgc taattcttct cttgatttat
     3301 ctcgtaagtc gtatgcttta ttgattaaat aaattccttg attcaatacg tccatcagca
     3361 tatatcgacc atacccccac tgataatgaa tcgacacgct tgctttcgtt ttgctgttac
     3421 catatttgac tgtaattaac gcttgtgttc tttcgcccat cttgttttcc tcttttctct
     3481 aatttttata aagaaccatt ttcttgaaat gagtaatagc ttgtgttttt acttgttacg
     3541 atgatatggt ctaacaattc aataccaatt gtttttcctg cgtttttcaa attcgtagta
     3601 aaacttttgt cattttcact tggaaattca tatccgctcg gatgattatg tgatactgcg
     3661 atttttgctg aattgtttag caaagctagt tgaaaaatct ctcttggtat cgctactgat
     3721 tgattaattc cacctttgaa aacttcgcta taagcaatga cctcattctt tgtattcaga
     3781 cacatcacta gcaaagattc actagcattg tttccaatta agttagtagc aaagtttgct
     3841 agaccaaccg agcttgtgac tctataaaaa cttctatctt cactgatata tttttgttcg
     3901 attttcaaga cattcttaat caatccttca ccttcattct cggtcttgtt ttcaacttca
     3961 atagattcaa gactgctatt tcccatatca gaacccaaca aagcatttct gaaatccaat
     4021 accttttcaa agttatcagt cataaccatt tcataggtgc tattccattc attcaaatat
     4081 ttctctttga tgagaatgaa cgctctcggt ttacttctgc caattctgat attttgtgta
     4141 tcgtgaaaaa tatagttatc aagtaaagta ccatcgtact tttcatagtt cccactgata
     4201 tttgttaact tgtttaccat ttcttgatta acttctaaaa ttctatctct catgttgaac
     4261 gcttctttct gtttttgatt tttggttttt caaaattctt ttttgtttgg attgttttag
     4321 agggaggtgg ttttttcttc atcgctattt ggttttgcat tttatgtttc ttcctttcgt
     4381 tcatttattt atctttttcg cctgtcttgc cttgtcggga gtgattagaa tgcgttctta
     4441 tctgccggat taatacaagt gtttaagtaa attattttcg taacgcttgc gtttagaaaa
     4501 aaatttacgc cacttgtatt aatccaaaga tgagataagc tctaaacact tctgacaagg
     4561 tatgacaggc gaaaaagata atgggcgtaa ggaattataa atggcaaaac caaataaaga
     4621 gcgatgaatt tcaatgcttt caggcgtagc ttgaaagcct aaaagtcttg aagaatgaaa
     4681 gactttttac cttcaaagca gacctagtaa tcttaaatag gcgatggcta tttaagatta
     4741 cggtgttgac ccactaaata aacagaaatg gaaccgatca agcaaaatcg gactaggcga
     4801 tggctaggac ggtttgcggt ggctttagga aacgttcatg gtgagatgag catatctagc
     4861 aatgtcgggc taagctatgc ttagaacggc ttgcggtggc tttagaaaac ttttatggcg
     4921 agatgcacta atctagcgat gtcggactag gcaatggcta ggacggcttg cggtggcttt
     4981 aggaaacgtt catggtgaga tgagcatatc tagcaatgtc gggctaagct atgcttagaa
     5041 cggcttgcgg tggctttagg agatgtttct atcatcatat ttcttcttaa atatagatga
     5101 atagaaaaat tacaagagaa ttttaagcat aaaaaaagag ttcaatattt agaactcttt
     5161 tttctaagat gctctggtaa catcttatcc aattcctctt ctgtaaaaaa ttgctcttct
     5221 tcagatttta atgctttctg agctgattca tagcttttac tatttgtttt aggggcattt
     5281 ttatatgctt catgagtttc gttcattaat tgatctcttt ttttcacaat atcttcaaca
     5341 cttaaacgat ttaaatctgt tagtaagctt acgtattctt ctctgatcaa ccataattta
     5401 tcggcaacaa tttgatgatt tttagcttct tcagcaagat caaaattctt aaagtataag
     5461 tttaatccaa gagaaattgt tgaacataat ccagcaacaa aggttaactc actattatca
     5521 gtaataatgg tactaagtac ccctccagta gtaagggctg acaaaccaat ttgaagacac
     5581 cttaaaattt tattgtcaga aataatatta tcggcttgct ttaaatgagt ggtgtaagta
     5641 tacgttactc gtccatatgc ttcacgtaat tgaacttcca agcgcttttt ttcgtctccc
     5701 atgttgttcc tccataaaac tttataaagc tatttttttt aatacttttt ctaaacatat
     5761 gatactacta tcagaattaa caaattgatt gcttccagga gagtataaat ttaaaccgcc
     5821 cataacttta tgtgagttat agtattctaa taaagctgtc tcataagata cagaactaat
     5881 gttttgaccc ccactgttat tttcaacgaa tttccaattt cccatagcct catatacgaa
     5941 actgtcaatt actattcctg aaagatgata actcgtaaat tctgtatctc gtacatgtcg
     6001 aaaatgttta cacgtagcat ataataaatt attacttttt gtattcttta atttcatggc
     6061 ttcttgctct tttttgggat ttgtagcttt ccagttccct cccatgtttg aatctggata
     6121 aataaaccct tttgactcac cccaataatc tttttcatta aaagctggaa ctatttcaaa
     6181 tttgattcca tcatggaaat ttattttaac aacttgtcca tcagctctaa tatcactatt
     6241 tggtagtatt tcctgaaatg cgttttttat aacttgtaat aacttagacg ggccatttga
     6301 taaataattt ttgaatctgt caaattctgc catcggtaat tcaactatta tatctatatc
     6361 actagtacta attgccgttc ctctcccata agaacctacg tataaactgt gagcagtatc
     6421 actttcagaa ttccaaaact ctctattgat ggctctagta atcctttgat atcttttagc
     6481 aattaattgg cgtttcgtta aaggtatagt ataagtatta gctctcggaa ccagtaaccc
     6541 cattataatc tcatcctttt taattaattt ttcttccata aattatgata tctaaatata
     6601 atacttcaag caatttattt tatatattac ttttataaaa aatttgatta attaaaggga
     6661 aatatttcac aataattgaa agtatttgga gaaaatttaa aaatatctaa gagactattt
     6721 tggtggatat ggaaatatat agtatctgtt cttatggtat cctgctccgg attcaatata
     6781 gaaacaaaag caatcaatcc ccacttcatt aattaattgt tgaccatatc cccataattc
     6841 ctctattagc tgttcatacg taatataacc gaattcgtat tttttaataa tgttcatatc
     6901 tgactccatt ttaataattc cgagaaagaa acattcctaa tttcatcata gtgaactttt
     6961 tcttcttcca caaatacacc aagctcgtca tatccaataa tttttcctac tacatcatct
     7021 ggatagtaac cttcagcgtt tcgttcctct ttttgaatgc agacgggtct attttttatg
     7081 acagcagttc tcagtatttt cgcaatctct tcagtcgtca tttgttcttt tgccaaatta
     7141 tgattagcac gttgtacatt gtctgcattg attctttcag tatgatcaga caaataaaat
     7201 cccgaccatt ttaaacgtcc tctatcgtta tattcaatca ttatgcaaac ctcctaatcc
     7261 tcctgcgtgt cctcctatta aagtgcttct cgtaatactt cttgcacctt ctaacataga
     7321 tgaagcatgt ataatagctg taaatccata tttttgtcta atcttatcaa taatcctatc
     7381 caaattttct tcagcaatct tttttcttgg atcactaaat aaatctaatt gtagggaatc
     7441 tgtgtatact aatctgctat aagttactcc tatatgtcgt acttcctgcc cctcataaaa
     7501 cttattgaat aaaaaaagac aataattttg taattctttc gtattatctg taattggtat
     7561 tttcatctgg tgcgagaaac ctgatttttc ttccaaaatt gacgtaccaa ttgctaagcg
     7621 tacacaacca gttttgcaat tgtgttgtct aattcgaatg gctacttgct ctgccatctc
     7681 cttgactact agttctactt cttctctcct cgtataatct tttggtagaa cttgtgaatt
     7741 cccataagac ttttctttgg ttggttctgg tggaatagca atatccgtac gatcaattcc
     7801 attggtatgg aaatataact ggagtccgat tacacctaac tgacttttaa ttgtgtaggg
     7861 attccaattt gctaaatctt ctatactcat aattcccata tgattcaatc tttttttcat
     7921 gcgtgatcca atgccccaaa agtcagtcat ctcgtttatt ttccatattt tttcaggtac
     7981 agtctcataa gtccactcag ctataaattc tttactactc ttcgcttcat tgtccagtgc
     8041 aagtttagct aataatgggt tatctcctat tccgactgtt gctattagac ctgtttcttt
     8101 ctgaatctca tctttgatta ttttagctaa ttctcttctt ctctcgcttc ttgttcctga
     8161 actatatagg ttgaattaaa tactttacat ccgcacgcac agtcggtcaa tcacctcaag
     8221 gggacgctcg tttacatcgg ctaagaaacc gcggaccgac tgtttgattt tctggacgtg
     8281 gtcaaaaaaa acattgttga tgaccgattc cttgagccac ttccaaacgc cttccattag
     8341 gttcaactga gg
//
LOCUS       DANKEF010000086         8283 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000086 DANKEF010000000
VERSION     DANKEF010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.086"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(51..1517)
                     /locus_tag="PWZ48_002534"
     CDS             complement(51..1517)
                     /locus_tag="PWZ48_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HDL2262152.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1904..2500
                     /locus_tag="PWZ48_002535"
     CDS             1904..2500
                     /locus_tag="PWZ48_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2262153.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2558..3040)
                     /locus_tag="PWZ48_002536"
     CDS             complement(2558..3040)
                     /locus_tag="PWZ48_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262154.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3146..3343)
                     /locus_tag="PWZ48_002537"
     CDS             complement(3146..3343)
                     /locus_tag="PWZ48_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262155.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3638..4396
                     /locus_tag="PWZ48_002538"
     CDS             3638..4396
                     /locus_tag="PWZ48_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HDL2262156.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4456..4884
                     /locus_tag="PWZ48_002539"
     CDS             4456..4884
                     /locus_tag="PWZ48_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262157.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4930..5002
                     /locus_tag="PWZ48_002540"
     tRNA            4930..5002
                     /locus_tag="PWZ48_002540"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4963..4965,aa:Gln,seq:ctg)
     gene            complement(5152..5523)
                     /locus_tag="PWZ48_002541"
     CDS             complement(5152..5523)
                     /locus_tag="PWZ48_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HDL2262158.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5738..5923
                     /locus_tag="PWZ48_002542"
     CDS             5738..5923
                     /locus_tag="PWZ48_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262159.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6006..6194)
                     /locus_tag="PWZ48_002543"
     CDS             complement(6006..6194)
                     /locus_tag="PWZ48_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262160.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6218..6481)
                     /locus_tag="PWZ48_002544"
     CDS             complement(6218..6481)
                     /locus_tag="PWZ48_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262161.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6639..6896
                     /locus_tag="PWZ48_002545"
     CDS             6639..6896
                     /locus_tag="PWZ48_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HDL2262162.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6967..7158)
                     /locus_tag="PWZ48_002546"
     CDS             complement(6967..7158)
                     /locus_tag="PWZ48_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262163.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7299..7619)
                     /locus_tag="PWZ48_002547"
     CDS             complement(7299..7619)
                     /locus_tag="PWZ48_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262164.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7642..8076)
                     /locus_tag="PWZ48_002548"
     CDS             complement(7642..8076)
                     /locus_tag="PWZ48_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HDL2262165.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
BASE COUNT         2625 a         1634 c         1313 g         2711 t
ORIGIN      
        1 aaaaacagga actgctgcaa ataagcaatt cctgttttta tgttcattga ttaatgaata
       61 gagcccatta gttttttgat tggttctttc acagtaaata gcaagatacc accgacgatt
      121 gctactgcac cgataatacc gaagtaagca gattctgttc ccggtgtgta gaagcgtgca
      181 atttgtgcat tgattgcttg tgaagaagca tcagctaaga accaaatagc gattgtttgt
      241 gattcaaatg ctcgtggtgc taattttgtt gtcacagaca aaccaactgg tgaaagacac
      301 aattcagcaa taaccacgat gaagaaactt cccaacaacc acaatggact tacttttcca
      361 cttgtaccat aaagcatacc cggcaacatc aatagcaagt acgataatcc tgcaaataaa
      421 agtccaagag agaatttcac tactgtagat ggttgacgtt caccaagttt cgtccacatc
      481 gttacgaata ctggtgtcag aataaagatg aaaattggat tcaatgattg ataccagctt
      541 gcttggatcg ttacaccaaa tagcgtagat tgtgtacgtt cactagcaaa taaagctaag
      601 atagatgaac cttgttcttg taatgaccag aaaatgattg ctgcaaggaa taatgggata
      661 taagcccata ctcttggttt ttcttcagca gttacatctt ttgaagtcag cattttgata
      721 aagtaataca acggtaagat aatccccaat acactgattg tattgacaaa aaagtcaatt
      781 gtcagatgac cagttagttg tgccccgccg aatactgcag cgataacaac aagtgcgatg
      841 atcaagttac ggatgaatcc tttacgttct tcagttgtca aaggattcgg cggtgttgta
      901 cccactccgt ctaatgtctt gcgtccttgg atgatgtatt gtaataatcc aaagaacatc
      961 ccaatagcag ctaaagagaa ccctaaatga tagttatatt cttgaccgat tgtaccaacg
     1021 attagtggtg ctaataatgc ccctaagttg acacccatgt aaaaaatcga aaatcctgca
     1081 tctcttcgca agtcagtctt tgaataaaga tgtccgacca tgccagaaac atttggtttc
     1141 aaaaaggcgg ttccgataat aatcaatgcc atcgaaacaa acaatgtact tacaccaaat
     1201 ggtaaagcca agacgatgtg gccaagcata atgaaaactc caccgaagaa tacagtgcgt
     1261 tttgctccta acactcggtc agatacccat ccgccgacga tactagacat gtagatcaaa
     1321 gatccgtaga tcgacatgat agctaatgct gttgctcgtg gtaatccaag tcccccgttg
     1381 gcaactgtat cgtacatata atagataagt aatgcacgca taccgtaata actaaatcgt
     1441 tcccacatct cagtgaagaa caaagttgac aatcccttcg gctgcccgaa gaagcccttg
     1501 tcttgctgtt tttccataat ctatacccca attctttaaa ttttcagaaa atattataca
     1561 tctttaccga aaaatttaac taaaaaactt atttaagaaa tataaactaa ttttctataa
     1621 aagcaagtaa tttttaatgg caatatcata aaaaaaagaa gatagagatt taataattat
     1681 gtatcagctg tttttttcaa ataagcaaaa attcctaaaa cacaacaatg aaagcgataa
     1741 ctaagaaaat ccttacataa cgatttttct tattaaaatt gttttttgaa cttcgcttat
     1801 tttttgtgag catatctgtt tacttacttt ttgtaaggtg ctatacttca tttcgtaatc
     1861 aattaaaaaa atgcaaaacg caaatcaaag gagaaatttt attatgacaa cttttactga
     1921 cactttgaaa aaccgccgtt caatctatca tctaggacgt aatgtttcac tttctaatga
     1981 agaattaaca actttgatca aagaagcaat taaagaaagc ccaactgctt tcaatgctca
     2041 atcaacacgc gcagttatcc ttttcggaga tgcacatgaa aaactttggg aaatcactga
     2101 agaagcactt cgcccattga ctccagcaga agctttccca aatacacaaa acaaattagc
     2161 tggtttcaaa aatggttatg gtacagtatt attcttcaaa gatactgacg tggtaaaagg
     2221 tctacaagaa caatttgaat tatatgcaga caacttccca gattggtcag aacaatcaaa
     2281 cggaatcgca acagctaata cttgggtagc attagttgat aaaggattgg gcgcaaactt
     2341 gcaacattac aacccagtta ttgacgaagc agttgcaaaa gaatggaata tcccttcaaa
     2401 ctggaaacta cgttcgcaat tagtattcgg ttcaccagaa acaccggctg gggaaaaaga
     2461 atacatgaac gatgccgatc gcttccgcgt atttggataa ttttatttga aataaaaaac
     2521 agaggttgtg acaaacatct tgtcgcaacc tctgttttta ttctggatca atcataatat
     2581 caatctctgc ctcatcgttt aagcgttggg acaaaaatgg aactaattct tctaatgatg
     2641 cagaaaattt cagcatcggt cctcgccatc cgtttcggaa aaaattcatt tttacgattt
     2701 tagaaccttt tggctgatat aatacaattt tattgatctc agaaaaagga acaccttttt
     2761 tgtcaaagct attcaaaaca agtccttctt ctgtgattcc tctactgtcc aacaaaaaac
     2821 taataaaaat cattgctgac aagatccctt ttgccaattc atctgctgta ggatgactga
     2881 tgagtgtata ccaaatgact agtgacacta agaagggtat caccaagtag acccatcgat
     2941 ttcgcacagt ccgataaata atttcttttc gaaccagcca aagaaataaa aacatcacaa
     3001 caacgaataa aaacaataga accgtaaaga atgttaccat gacaactcgc ctcgcttttt
     3061 cttctccaga aaataggaaa agaatactga tttaccttct caaagaaatt ggacttgcag
     3121 cacgccgcac gtccaattgt tttctttatt caaatgtatc atattttgca acaaccatgc
     3181 ttgctttttc atctaataat tcttggtcct ctgcagcaca ttgggcaact ttgaccacgg
     3241 cgcagtttgt catttttgat acaacttcac caatgaccgt ttttgtaaaa ccaattggtt
     3301 tgcatgcata ttgttctcct aaattaatca tttcttccgt catcaaaatc ctcctaaact
     3361 gctggaactt ttctccacat taccactgct atttctttgg agatttaact cattgatttt
     3421 tatttacagt taaaaatcaa ccttacaggc attatagcgt actgttttcg ttttcacaat
     3481 taaaacagct gttgtattta gaagaatttt tttcaaaaaa ttaaaacata ttccttaaaa
     3541 actaattatt tcctacatat taaaatataa tacttccatt tccaataaaa atacatttat
     3601 aataggaaag ataaacagct gaaaagggag atttcaaatg aagaaaaaaa cgttcaaaaa
     3661 actattgttt ctagggggca cagctgctct tttattaagc ggtacaagtt attacctaca
     3721 tatccattct ccagaacata caatgaacac tgcacaaaaa agttcaacac aaaaaacgaa
     3781 aaagacaaaa gagaaagcag aagttttact agatgttcca ttagagagcc agtttgattc
     3841 tccttcttta gaaaatggct gtgaagtcac ttcgctttct atgcttttat ctttttatgg
     3901 ttatgaaaca actaaaaacc agttagctga gcaattggat tacgtccctg ttttcaatgc
     3961 agatgggact catggcgatc ctaatgaagg atttgttggt gatatcagtg gcggagactg
     4021 ggctatggtc gtctatgttc ccccagttgc ctcccttgct cagcaaatcg tccaaacgga
     4081 ttatcacact tttccaaaaa ccgatgctca actcgatgat atcaaaaaag cactatctaa
     4141 aggaaatcct gtctggactt ctgtgactat caattttgaa gtcccagatg aaactgattt
     4201 tatgacttgg accacaaata atggagaagt aagagtcact cctcttgttc atgcttgtgt
     4261 agtcactgga tatgacaaag aaaatattta tgtcaacgat ccctatggtg taaaaaatcg
     4321 agcagtaccg atcaacgatt tttcggcgat tttcactgca atgggtggac agatgctaac
     4381 tttggagaaa agctagtaat tcaaacaaaa atcccttata ctcaagatac caaacctttg
     4441 aagggagcgt agataatgga aaaattcaca cataaaaaaa tggatccaaa tgaaattccg
     4501 attatttttg tccgtgactg caagggaaat gttcaaggta aagtctctat taatgagtgg
     4561 aacgagcgtc gcagacctgc taccctcaat gaactggaaa tcaaattgta tcgtcaatct
     4621 cttgtttatt acgcagatca agagtacgag aaagccaccg atttattgaa atttctaatt
     4681 gctcgaacag agtatactcg ctttgaatat attgaacgtt tagcaaacat ctatcatatt
     4741 atgaatgaac ctgtcaaaga atatcagtta ttggacaccg tcttgtcagt agcagaactg
     4801 attgcccttc ctgctggatt agaaaagaaa ctagtacgcc gccttttacg agtaaagcaa
     4861 caactatccg atcaagaaaa ataacttgcg tttttttagg aaagtgctat gattattttg
     4921 ttgaataaat gggatgtagc caaactggta aggcacttga ctctgaatca agcaattgta
     4981 ggttcgaacc ctgccatccc aatttgatgt gcttgctgaa aaaatgaacc ccctgtaaaa
     5041 gaacaatctt tttgcagggg gattattgta ttatttttac tacttgtatt tagtttacat
     5101 aattaaatta tgaataataa aatcgggcgt attcttaata caaaaactga tttacccaat
     5161 aattgctaat gctttctccg gaactttttc tctaggaact tcctcatacg tttctatgtg
     5221 tgctccttta ttgctaattt tcaaataatg attttctttt aacacagaaa gaccattaaa
     5281 ttcaaccgtt cgttctttcc cattggcatc agcagctttc tgcacatacc ttgctgtacc
     5341 atattcattt acttcctctg gtttttgtgt ctttacatag acctctcctt tttcaactaa
     5401 tggatttagc tgatcaagta taccagggat ttctccataa cttccttcag tataatattt
     5461 cagtccaaat agcaaaatac cactaatcgc cgccagtcca atcaaatatt taatcacttt
     5521 catctttcta cctccttgtt atatattcag tatatgggtc agttaaagaa attccatccg
     5581 tcccaagttg gagatttttc tcctacttta gaagcgagaa tcgagtgttt ttttagtaaa
     5641 aatgatcgtt atttgatatt attgacgtgt ggataaatca gtttttctcg ttttgtccat
     5701 gtaaaaaatc atgttgatag caaaggatgt gttcaaaatg aatcaagaag aattatttca
     5761 aaaagtcaag gaaatgatta agaacggcaa tttcgatggc gcaaaacgat ttatcgaaga
     5821 acataaagaa caattaggac cttataaaga gaaagcccaa aatcttttaa aagatgtaaa
     5881 tatcgacagt gtcaaaaaca aatttaaaaa tctattcaaa taagtaaaac tttcactttt
     5941 gtaaaatcaa aggaggacaa tgctgctgca tcgtcctcct ttggttcttt tctgaatact
     6001 tcatactaaa ataaacgtgt cggatcgtaa tgataagtca cattgccgtt cagtgaggct
     6061 tttcccacca catatttacg attctccaac ttcagccacg cttctcgaaa agccattaat
     6121 tcttctcttt ttacatcaat cttttcgatt tttccatcga tcaaatcttg aataagttgt
     6181 tcatatggat tcattttttt cctccatctg ctgtcaatca ataagttgta aaaataggtt
     6241 catatgcttt tttggtatct agatcttctc tgactttgac tgtcgtataa ataatttttt
     6301 ctactgggat actataataa cttccttcac cattattgaa tcggtgaacg tctttctttg
     6361 ctaaaatacc atttatttta ttcgtcagta cacgttgttc gacactgtta cttacaacat
     6421 agtaaatcac ccgaatatta ttgatcaaat gcaaatcgat ttctaacact acatgatcca
     6481 tgaaaatccc actccttctt tcactttgat tatagcaagt ttctcctcta atttcaaaat
     6541 cgatacagat tttaccggat aaatccacaa aactgcttgc gccatttcgc acaaagtgtt
     6601 attttgtatc atggacttta tttatcggag gcaatagtat gatcgcgatc ggtcaatttg
     6661 ttttttatat tccttttttt attatgataa gcattctttt ctattacata aaatggacaa
     6721 aaaagaagtt ttcagtttta ctggcctctt tacctgctgt atattttact tatcaaattt
     6781 tttcttttag gcattgggaa acaacgtctg tattaataac acacatcatc gaattgacgc
     6841 tttcagtaat ttttcttatt atttggatct attttttata taagaatcag aattgaatcc
     6901 ctcgcatgtt taaaaagaaa acgattaaag atgcagcaaa tttctgccga tctttaatcg
     6961 ttttttctat tctgttatac caaaaaaatg tttgaatcgg tgaaggatcg ttccttgttt
     7021 taattctata aattcttgtg cgatcgggta cttttcttcc aaattttctt tgttcttttc
     7081 gatcaggctt ttcgcttctt catatttttt atctgcacat agtgctttta cttcctgtaa
     7141 tacttttcct gcttccattt gcctcaccct cttgccattt tattgcttta agttttattt
     7201 aagttatacg cattattcta gtttcatccc aaataacctt ttttcatttt tactcctcga
     7261 actaatcagt tttctaaagg ctgattggtt cgtctttttt atttttcctc catttcattc
     7321 tgctcttctg ttaatttatc taatgctttg attgatgctg cggctcttgc ataatcatag
     7381 ctgtataatt ccataaattt atcaggcaga acaaaccacg ctggatgata accgttagct
     7441 gtgacaggga atcctttatc ttgttggtta tctccatccc atctcagagc aacacctttt
     7501 tttcctttcc actttactaa agcgatcgag tatcccttct ttgtttgtgt gccgtcttca
     7561 ataattgcta aaatttctat tttttctttt ggcgcacgga cttcttgagg acgaaccatt
     7621 ttctattcct cccttatctc attacttcac gaccaacaca ctgcaagggg catgtgatac
     7681 gacataagaa gcggtcgatc caactaatgc ttgttgtata gcaccttttc ctgtcgctcc
     7741 cataacgatc aaatcaatat tttcttgtcc agggatgaca ttggcaatca aatttcttgg
     7801 atctccagtt tcaactattg cgtggatctc agcaatgccg tactctttgg catcattgat
     7861 ttttttcagc atggctactt ctgttctttg tttttcatct gcatagattt ttgaaaaggc
     7921 ataggcacta tgggtcagct caactttgtt gatgattgac acgataaata agtgggcttc
     7981 attgtccttt gctaagtcaa gggcttcagc gaacgctttg atcgattgtc ttgatccgtc
     8041 aactgctacc atgatgttct ggtatctttt actcattgga accgcctcct ttttctttta
     8101 ttgtaacatc tcaagcaaaa atacaaaaaa tatacgcaat atgtaaataa aatgatgaac
     8161 aaaagcaatt tctttcaagt tatttataca tttcttttct catcagaaca cctgttctaa
     8221 tcccattatt tctgcattat gtgaaattcc tcatcatttt tcacatcgag cgaacatcat
     8281 tct
//
LOCUS       DANKEF010000087         8268 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000087 DANKEF010000000
VERSION     DANKEF010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.087"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            16..840
                     /locus_tag="PWZ48_002549"
     CDS             16..840
                     /locus_tag="PWZ48_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2262166.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            872..2182
                     /locus_tag="PWZ48_002550"
     CDS             872..2182
                     /locus_tag="PWZ48_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HDL2262167.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            2330..3589
                     /locus_tag="PWZ48_002551"
     CDS             2330..3589
                     /locus_tag="PWZ48_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HDL2262168.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            3599..4471
                     /locus_tag="PWZ48_002552"
     CDS             3599..4471
                     /locus_tag="PWZ48_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2262169.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            4483..5313
                     /locus_tag="PWZ48_002553"
     CDS             4483..5313
                     /locus_tag="PWZ48_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2262170.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            5353..7536
                     /locus_tag="PWZ48_002554"
     CDS             5353..7536
                     /locus_tag="PWZ48_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDL2262171.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            7597..>8268
                     /locus_tag="PWZ48_002555"
     CDS             7597..>8268
                     /locus_tag="PWZ48_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HDL2262172.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASL"
BASE COUNT         2734 a         1253 c         1648 g         2633 t
ORIGIN      
        1 ggaggctatc aaattatgaa aaataaattt ttttcaaatg cgccaatcca tatcatgctt
       61 attttaggtg gtattgcaat ggcattacct tttatttgga tgattttaac ttcagggaaa
      121 acgttggttg aatctaccca aattccacct acaattttcc ctgagaaatt tcagctaagt
      181 aactatcaac aagtacttga tttattacca ttcatggctt tttatatcaa tacattttta
      241 atggttgttg gacgagtgat tggctcggtt ttattttcag caatggctgc atatgcttgt
      301 gcgcgtctaa gttttccagg gaaaaatatc cttttcagct tagtacttat tcaaatgatg
      361 attcccggtt ccatttttat tactccacag tatttattag cacaaaaatt gggtatttta
      421 aatacaattc cagcattaat attaccaggg gtagttagtg cgtttggtac gtttttatta
      481 agacaatttt tcttaggttt acctgatgaa ttggaggaag cggcctattt agaaggtgcc
      541 aatatttgga cgatcttttt caaaattatg ttgccgcttg ctaaatcagg attggttgca
      601 cttggaatat tcacagcgtt gtttgcattt aaagatttaa tgtggccatt gattgtcaat
      661 acttcaattg ataagatgcc tttagctccg ggattagcaa acttacaagg acaatatgcc
      721 acgaacttcc cacaattaat ggcaggttcg ttattagcaa tttggccaat agttgtgata
      781 ttcatcgttt tccaaagaaa attcattgaa ggaattgcta caacaggtag taaattataa
      841 aactattaat tagtgattgg agagtaagta catgacaaaa attacattta ttggtgcagg
      901 aagtactatt tttgcaaaaa acgtattagg cgatgcgatg ttaactccaa gtctacaaga
      961 tgcacatatt gcattgtacg atattgatga aaaacgacta aaagaatcaa atttaatgct
     1021 taaaaatatt aataaaaatt cgaatgaaaa tcgtgctgat attgtttctt attatgatcg
     1081 caaagaagcg ttaagagagg ctgattttgt tatcaatgct attcaagtgg gtggttataa
     1141 accttcaaca gtgattgatt ttgatattcc taaaaaatat gggttgcgac aaactattgg
     1201 tgacaccatg gggattggtg ggatttttag aaccctacgg acactaccag taatgttaga
     1261 ctttgctaaa gaaatggaag aagtttgtcc aaatgcttgg ttattgaact atacaaatcc
     1321 gatggcgatg ttaaccatgg gtgttttaaa agcgacgagt attaagactg ttggtttatg
     1381 tcatagcgtt caagtatgcg taccagaatt gtttgagcat ttaggaatag ccgatcaata
     1441 tgacttaaat gactttcaat ggaaaattgc aggcattaat catatggcat tcttgttgga
     1501 aattacaaaa aatggggaag atttctatcc aacaattcgt cagttagctg cagaaaaacc
     1561 aaatccacat ccagattctg tacgttttga attgttgaaa cgttttggtt actatattac
     1621 agaatcaagt gaacacaatg cagaatacca tccatacttt attaaatcaa aatacccaga
     1681 attgattgac gaattaggga ttccgttgga tgaatactta cgtcgttgtg aacaacaaat
     1741 taatgattgg gaaagcatgc gagaggacat tgtaaataat ggggagttaa cacatacgag
     1801 aagccgtgaa tatgcttcat atatcatgga ttctattacg acaggaactc cgtcagtgat
     1861 tgcaggaaat gtcttaaata aaggcttaat cacaaactta ccgacagatg tttgtgtgga
     1921 agtaccttgt ttagtcgata aaaatggcgt gcaaccaacc tatgtcggtg aattgccaac
     1981 acaattagct gctttaaatc ggacgaatat caatgttcaa gaactgacag tagaagccgc
     2041 tttaacacta gataaatcaa aaatttatca agcagcgtat ttagatccac atctatcatc
     2101 tgaattgtct ttaacagata tcaaaaattt agtggatgat ttaatcgaag cacatgggga
     2161 ctatttacct gaatataatt aaaaagaagc tgaaagagta aggaattttc aattccttac
     2221 tcttttctta gttaaattta gtaaaaatat tgacaaaaca cttagtaaac agttacatta
     2281 gtgttaacaa aaagaaaacg cattcaaatt gtataaggag gagcataaaa tgaagtggtc
     2341 taaaaaaatt attttagctt gtatagcact aggagcaacg atctctttga ctgcttgtgg
     2401 aaaaaatcag gaagatgcaa gcggtaaggt agaaatagag ttttttaatc aaaaaaaaga
     2461 aatgactcaa acgattcagg agattgctaa agattttgaa gcaaaaaacc ctgatattca
     2521 tgtaaaagtt gtggatgtac cgaatgctgg tgaagtaatt aaaacacgta tgcttgcagg
     2581 cgatgtacca gatgtcatta atctttatcc tcaaagtata gaattgaaag aatgggcgaa
     2641 agctggttat ttagaggact taaccgaaga accgtattta gagaatatca aaaatggata
     2701 tgcacaacgt tttgctattg aggatcgtgt atatagcata ccattaaccg ctaatgtcta
     2761 tggtttttac tataacaaaa ctgcttttga ggaaatgggg attcaagcac cagaaacttg
     2821 ggcagaattt gaaaaaatag tagctgagat taaagatcaa aacaaagtac cgtttgcgat
     2881 tgcaggagca gaaggctgga ctttaaatgg ttatcaccaa ctagctatag cgactgctgc
     2941 tggtggtgaa gaggaagcga acaatgtttg gcgtttctca gaagttaatg gaattaatgc
     3001 tgaaagcaaa gaaatgcaaa aagattttaa tcgattagat ttgttaagag aaccaaaagc
     3061 tttgcaaaac aactggcaag gtgccggtta taacgataca gtagttactt ttacaaaagg
     3121 tgatgcgttg attatgccga atggttcatg ggcaatgccg atgattcatt cacaaaatcc
     3181 agattttgaa gtaggaactt ttccttttcc agcagatgaa gcaggtcaaa gtctaacgat
     3241 tggcgcagga gacctagcct tatcagtttc tgcaacttct caacataaag aagaagccaa
     3301 acgttttgta gaatatatga caacacctga agcgatgcag aaatattatg atgtagatgg
     3361 tgcaccgtgt gctgttgagg gcgttatcga gaatactcag gattcaccat taagtggttt
     3421 aactgaactt gcttttactg atcgtcattt agtttggtta gccaaagatt ggaatagtga
     3481 aaatgatttt tataccttaa caacaaatta tttacacaat ggcaatcaaa aagccatgat
     3541 taacgcatta aatgctttct ttaatccaat gaaagctgac ggaaggtagg aaaataaaat
     3601 gaaaataaaa gaaattttaa atcgttactg ggcaatcatt tttgtagcaa ttccaattat
     3661 tttgcagatg atttttttct actttccaat ggttcaaggt gctttttaca gtttaactaa
     3721 ttggactggt ctgacttata attttgattt tgtgggtttg aacaattata agattctttt
     3781 ttctgatccg aagtttatca aatcgattgg cttcacttta attgtgacgc tttgcatgat
     3841 tgctggacaa attggttttg gtattttgat tgcgcgggcg ttgaacgcaa atattaaagg
     3901 gaaaacattc tttagagcat ggttcttttt tccagcggtc ttatcgggcg tggttgtttc
     3961 tttagtattt aaacaaattt ttaactatgg tttaccaaaa attggtagta tgttaggcat
     4021 tgaattttta caaaccagtc ttttaggaac aacatgggga gcgatttttg cagtaatttt
     4081 tgttttactt tggcaaggaa ttgccatgcc agtcatcatt ttcttagcgg gttttcaaag
     4141 tattccacaa gaaattattg aagctgctag aatggatggg gcgaatgata agcaaatttt
     4201 ttggaatatt gaaatcacct acttattacc aagtatttca atggtattta ttatggcatt
     4261 aaaaggtggc ttaacagcat ttgatcaaat ttttgcttta actggtggtg gaccaagcga
     4321 tgccacaaca tctttggggt tacttgttta taattatgct tttaaaaaca atcagtttgg
     4381 ttatgcgaat gctattgctt tagtcttatt tatcttaatt gggattgttt cactggcaca
     4441 attaaaatta tcgaaaaaat ttgaaatcta ggaggtttcc caatgaaaac atcaaaagaa
     4501 aaaaatattt ctaaatactt gcttttatta ttagggggct tcatgatttt agttccctta
     4561 gtttcaacgc tattcggttc atttaaaaca acacccgata ttatgaataa tttctttcgt
     4621 tttcctaatc cagcgacttt ggaaaatttc cgaagattgt tagcggatgg aattggtcat
     4681 tacttttgga attcggcaat catcacaatc attgcagtag ttgctgtgac agcaattatt
     4741 cctatggctg cttattcact agcaagaaaa atgactcgtg ggaaagcata cggattgatg
     4801 tatttatttt taattttagg aatttttgtt cctttccaag taattatgat tccgattacc
     4861 gttatgatga gtaaaattgg tttagcaaat atgtggggat taatcttact ctatctcact
     4921 tatgccgtac cacagacact atttttatat gttggtttta ttaaactaaa tgtgcctatc
     4981 agtctggatg aggctgctga aattgatggg gctagtcact ttacaactta tttcaaaatt
     5041 gttttcccaa tgttaaaacc aatgcatgcg acgactatta ttcttaatgc tctatggttt
     5101 tggaatgatt tcatgctacc attattaatt ttaaatcgtg attctaatat gtggacacta
     5161 ccattgtttc aatataatta tacgggacaa tattttaacg actatggtcc aagttttgct
     5221 tcctatacag taggaattgt cacgattaca ctagtttatc ttgctttcca aagaagtatt
     5281 atagctggaa tgagcaatgg agcagtgaaa taaccaataa attattctca ttataaagga
     5341 gttcataaac ttatgattat atttgacaat cacacacaaa catttcattt acaaaataaa
     5401 aaaattagct atctattagg aatcgaagaa aataattatt taacgcatct ttattttgga
     5461 aaaaagatta cgcactattc tggtgggtat cgctaccccc gaacagatcg ttccttctca
     5521 cccaacaatg caaatgcgca agatcgttta ttatctttag acacattacc attagaattt
     5581 ccaggatatg gacatggtga ttttagagaa ccggcgcata atattaaatt aggaaacggt
     5641 agtcgtgtaa atgattttcg ttatgatagc tacgaaattc aaaaaggaaa gcccagctta
     5701 gcgggattgc cagccttata tacggaagat gatgatgaag ctgaaacgct gattattcaa
     5761 ttgaaagacg cgattagtgg attgacttta cagttgttct acacaattta tcaaaatcac
     5821 agtgtaatta ttcgttctac gcaattagta aataatagtg atgaaacagt ggagattaac
     5881 cgcttagcta gtcaatcaat tgattttcca aatcgtccac tcgagttaat tcatttaaat
     5941 ggtacctggg caagagaacg tcaaatgact agagaaaaaa ttcatacagg gataaaagta
     6001 ttagatagta aacgcggatc aagcagccat caacaaaatc cgtttgttgc tttagtagat
     6061 gccaaaacag atgaatttca aggagaggca tatggattta atttagttta tagcggtaat
     6121 cacgaaacgg tgattcaaaa agatccattt gaccaaacac gggtcattat gggaattaat
     6181 tcatttaatt ttggttggca actaaaaact ggcgactcat ttcaaacgcc agaagtcgtg
     6241 atggtttata gtaatgaagg tctgaatacg ttatcacaga cgtatcatga attgtataat
     6301 caccgtttaa ttcgtggaaa ctatcgttta aaagaacgtc ccacgttaat caacaactgg
     6361 gaagcaacgt attttgattt taacgaagaa aaaatcatga atattgtcga tgatgcgaag
     6421 aacttgggca ttgaaatgtt tgttttagat gatggttggt ttggcggtcg tgaaaatgac
     6481 ttcacttcat taggtgattg ggttgaaaca gaaggcaaac ttgcttgtgg tttggaaaat
     6541 ttagccaagc aagtgcatga taacgggatg aaatttggga tttggtttga accggaaatg
     6601 atttctgaag atagtgaact atttcgagca catccagatt gggcattggg tattccaggt
     6661 cgtgaccgct caattagtcg aaatcaatat gttcttgatt tttcaagaca agatgttcgt
     6721 gataatatct atcaacaaat gacggcgatt tttgatcgtg taccgattga ttatgtgaag
     6781 tgggatatga atcgaaatat gacagaagtc tattctgtct tgttggactc aacgatgcaa
     6841 ggtgaagtgg ctcatcgtta tatgttaggg ttatatgaat ttttagaaaa gttaacaacg
     6901 aattatccaa ctattttatt tgaaagttgt tcgggtggag gcggtcgttt tgatgcaggt
     6961 ttcttgtatt acatgccaca aacttggacg agcgacaata ccgatgccgt ggctcgtctg
     7021 aaaattcaat atggaaccag cttagtgtat ccaatttcaa gtatgggtgc gcatgtttca
     7081 gcagtcccaa atcatcaaac aggaagagaa acgagtttaa ctattcgtgg caatgctgcg
     7141 atggctggtg tttttggtta tgagctagat ctaacgcaat taacttctga agaaaaagca
     7201 gaaattaaag aacaagttgc cttttataaa aagcatcgcc aacttttgca atatggaact
     7261 ttttatcgct tggaaagtcc gtttgaaggc aatgatactg catggatgtt tgtctcaaaa
     7321 gaccaaacag aagccatggt cttttatttc cgagtattag cagaagctgc gcatccatta
     7381 gtcacattga aattagcagg attagcagaa gaagcaactt atcaaatgga agatgtaaca
     7441 tttgctggcg atgaattgat gaatctcggt ttttatattg actcagaact acatggtgat
     7501 tatgcgacac aacgctttta tttgaaacag atatagtagc ttgaatttac aatataagtg
     7561 caacaccaaa agagactcat tccaaacgaa gaaaatatgt tttatttaca cgcatttact
     7621 aaaaagcagc ctgatttgaa ttgggaaaat gaagaagtac gtgaagaaat ctatgcaatg
     7681 attaactatt ggttagataa aggactaggc ggatttagaa tcgatgcgat attaaacatc
     7741 aaaaaaaaac tagagtatgg catctttgaa tctgatggag aagatgggtt agccttcatt
     7801 gggccctgga ttcttaatca gtcgggcatt gaagtgtggt taaaagaaat gagagagcgg
     7861 acatttaaac cacataatag tatgacggta gcagaagccg atgttcctaa tgaacgttta
     7921 gatgaatata ttggtgaaga tggctattat tcgatggttt ttgattttag ttatacggat
     7981 attgatgtac ctgaaactgg tgaatggttt aaagatagcc aatggacatg gacggatatg
     8041 cgtaccaata tttttacgaa tcaattagtt acgcaagata aaggttgggg cgccttatat
     8101 ttagaaaacc acgatcaacc acgttcaatt aataaatata ttccagaaga atggatcaat
     8161 gattatagta aaaaaatgtt ggcgacgctt tttatgttac taagaggtac cccttttatt
     8221 tatcaaggac aagaactagg aatgaccaac atagaaatgg catcatta
//
LOCUS       DANKEF010000088         8251 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000088 DANKEF010000000
VERSION     DANKEF010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.088"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..197
                     /locus_tag="PWZ48_002556"
     CDS             <1..197
                     /locus_tag="PWZ48_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262173.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="PWZ48_002557"
     CDS             314..811
                     /locus_tag="PWZ48_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2262174.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="PWZ48_002558"
     CDS             898..1290
                     /locus_tag="PWZ48_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2262175.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..3721
                     /locus_tag="PWZ48_002559"
     CDS             1274..3721
                     /locus_tag="PWZ48_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2262176.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKAERGRWK
                     ETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESLAIDILTFLTGISSRDGER
                     FPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADHIESFTDYDFAHLLFSDGY
                     VEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVAMLIVISTFALDFIHTDRS
                     IFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFVTQNTDDLLDEKLKNNLGL
                     KFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQCLISDLYGRVGVIQFHPVF
                     EELLHAFDTRPPVRKEV"
     gene            3724..5907
                     /locus_tag="PWZ48_002560"
     CDS             3724..5907
                     /locus_tag="PWZ48_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HDL2262177.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            5904..6905
                     /locus_tag="PWZ48_002561"
     CDS             5904..6905
                     /locus_tag="PWZ48_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HDL2262178.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            6920..7834
                     /locus_tag="PWZ48_002562"
     CDS             6920..7834
                     /locus_tag="PWZ48_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2262179.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            8079..8195
                     /locus_tag="PWZ48_002563"
     CDS             8079..8195
                     /locus_tag="PWZ48_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HDL2262180.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
BASE COUNT         2569 a         1568 c         1754 g         2360 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagcag aacgtggcag atggaaagaa accttgccag agatttccca
     2821 tgaaatcaat atcgtcaatc tgacttctga tgagaaaaac aaaggcttac ttgaccctta
     2881 tgtaattatg aaaaatacca aagattctga atcactggct attgatattt tgacattcct
     2941 tacggggatt tcctctcgtg atggggaacg cttcccaatc cttagaaaag ccattcgtgc
     3001 agtaaccaat agtgaagtgc gagggttgat gaaagtgatt gaagaattac gggttgagaa
     3061 tacgccacta agtaccagca tagccgacca tatcgaaagt tttacagact atgactttgc
     3121 ccatctgctt tttagtgatg gttatgtaga gcagtctatc agccttgaaa aacaactgaa
     3181 cattatacag gttgccgact tggtacttcc tgacaaggaa acttcctttg aggaatatac
     3241 cacaatggag ttactatcgg tagcaatgct gattgtcatt agtacctttg cgttggactt
     3301 tatccataca gaccgaagta ttttcaagat tgtagactta gacgaagcat ggagcttctt
     3361 acaggtggca caagggaaaa cactatctat gaagctggtt cgtgctggtc gtgctatgaa
     3421 cgctggggta tatttcgtga cccaaaatac agatgacctc ttagatgaaa aactgaaaaa
     3481 taacctcggc ttaaaatttg cctttcgttc cactgacctt aacgagatta aaaaaaccct
     3541 tgcctttttt ggtgtagacc cagaggacga aaacaatcag aagcgattgc gtgatttgga
     3601 aaacgggcaa tgccttatca gtgatttata tggtcgtgtc ggtgtgatac agttccaccc
     3661 tgtatttgaa gaactgctcc atgcctttga taccagacca cctgtgcgaa aagaggtgta
     3721 aatgtgaaac catcaatatt aaacagaata aaatcaaact ggacgctgaa acgtctaggt
     3781 aaagtggcaa tgacagtggc tttcacactt gtgattgcca tttttctttt agccatgctg
     3841 ggaacggtgg ttcaagctgc gggcttggta gatgatacgg tcaatgtggc aaatgaatac
     3901 agccgatacc cacttgaaaa ctatcaactg gatttttatg tggataatag ctggggctgg
     3961 ctaccgtgga actggtcgga cggaattgga aaacaagtca tgtatggact atatgccatt
     4021 accaatttta tttggacaat cagtctttat gtttccaatg cgacgggtta cttagtacag
     4081 gaagcctatt ccttagactt catttccgct acagcagatt ccattggcaa gaatatgcag
     4141 acccttgcag gggttacgcc tagtggacta tcaacagagg gtttctatgt tggattcctt
     4201 ttactcttga ttttggttct tggggtttat gttgcctata caggactgat aaagagagaa
     4261 accacaaagg caattcatgc cctcatgaat tttgtgctgg tttttatcct atcggcttcc
     4321 tttattgcct acgctcccga ctacattaaa aaaatcaatg acttttcatc agacatcagc
     4381 aacgctagtt tgtcactggg tacaaaaatt gtcatgcccc attcagatag tcaaggcaag
     4441 gacagtgtgg acttgatacg tgacagccta ttttccatac aggttcagca accgtggcta
     4501 ctgcttcaat ataacagttc agacattgaa agtattggta ttgaccgtgt ggaaagcctg
     4561 ctctcaacca gtccagattc caacaatggg gaagacagag aaaaaattgt tgcggaagaa
     4621 attgaagata gaagcaatac caatatgacg attacaaaga cgattaaccg tttaggtaca
     4681 gtcttcttcc tatttgtctt caatattggg atttccatat ttgtattcct attaacagga
     4741 atcatgattt tctcgcaggt actttttatc atctatgcta tgtttctgcc tgtgagcttt
     4801 attttaagca tgattccatc atttgatggt atgtcaaaac gagccataac aaagctcttt
     4861 aataccattt tgacacgagc tggaatcaca ttgattatta cgacagcatt tagtatttca
     4921 accatgctct ataccttatc ggctggttat ccgttctttt tgattgcttt tctacagatt
     4981 gtgacctttg caggaatcta ctttaagctg ggcgatttaa tgagtatgtt ttctctacag
     5041 agtaacgatt ctcaaagtgt gggaagtcgt atgatgagaa aacctcgtat gcttatgcac
     5101 gctcacatgc accgtctaca gcggaaactt ggacgctccg tgactgcttt aggggctggg
     5161 tctgccattg ccagtgctac gggtaaaaaa ggacagtcgg gttcgggaag ttctgcgagt
     5221 acacaagcag atcactctcg accaaacggg caggaaaaat caacactagg aaagcgtatc
     5281 ggtcaaacca tcggtacagt agctgatacc aaagacagaa tggtagatac tgctggtaat
     5341 ttgaaagaac aggttaagga tttgccgacc aatgcaagat atgcagtata tcaaggaaaa
     5401 tctaaagtga cggataatgt ccatgattta accagaagta tttctcaaac caaagcggat
     5461 aaagccagtg gacgcaagaa acagcaggaa caaagacgga aaaccattgc ggagcgtcgc
     5521 tctgaaatgg aacaggtcaa acagaaaaaa cagccagctt cttccgttca tgaaagacca
     5581 gccactaagc aagaacaatc tcatgatgga cagacttcaa ggcaaacaac tgtacaggct
     5641 tcctataggg aatctcaaca agccaaacaa gagcgtccaa cagttaagtc cgattcttca
     5701 agtttaaaaa cggaacgtca aagtaataca gttcaagaaa gaacggttca aaagccagta
     5761 acttcaactg caccaacaga tagagcttca caacgttcaa tcacaaaaga acgtcagtct
     5821 actgttcaaa gagtaccact acaaaataca aaaagtagac caccaatcaa aaccgccacc
     5881 attaagaaag gcagtaagaa accatgaagc tgaaaacttt agtgattggt ggttcgggat
     5941 tattcttgat ggtcttctca ctgcttctgt ttgtcgccat tttattttca gatgaacaag
     6001 atggcggttt ttccaacatt cactatggcg gtgtggatgt ttccgcagaa gtgctggctc
     6061 ataagcctat ggtagaaaaa gttgccaaag aatatggaat tgaagaatac gtcaatatac
     6121 ttcttgcgat tatacaggtg gaatcgggtg gtacagcaga agatgttatg cagtcctcgg
     6181 aatccctcgg tcttccacct aattcattga gtacagaaga atccattaag caaggtgtga
     6241 agtatttcag tgagttatta gccagtagcg aaaggcttgg tgtagattta gaatcggtta
     6301 tccagtccta caattatggt ggtggtttct taggatatgt ggctaatcgt gggaataaat
     6361 atacctttga actggctcaa agtttctcaa aagagtattc gggtgacgaa aaagtgtctt
     6421 accccaatcc catagccata cctatcaatg ggggctggcg atacaattac gggaatatgt
     6481 tttatgtgca actggtaacg cagtatcttg tcacaacaga gtttgatgat gatacagtac
     6541 aagccatcat ggacgaagct ctgaaatttg aaggctggaa gtatgtctat ggcggagctt
     6601 ccccgactac ctcttttgat tgtagcggac tgacacaatg gacgtatgga aaagctggaa
     6661 tcaacttacc acgaacagca caacagcaat atgatgtaac ccggcatatc ccattattgg
     6721 aagcaaaagc cggcgatttg gttttctttc attctaccta taacgctggc tcttatatta
     6781 ctcatatcgg gatttattta ggcgataacc gtatgtttca tgcaggcgac cctatcggtt
     6841 atgccgactt aacaagctct tactggcaac agcatttagt gggagcagga cgaatcaaac
     6901 aataagaaag gacgattcaa tgatgaaatt cggaaaaaat cagaatacag aaaaacagac
     6961 accaaaggaa aagaaacctc gtgtctataa ggtcaatcct cgtaaaaagg ttgtgattgt
     7021 cctatgggta cttttaggac ttagtttcag ctttgcgata ttcaagcact ttacagccat
     7081 tgatacccat accattcatg aaaccaccat catagaaaag gaatatgtcg atactcatca
     7141 tgtggaaaat tttgtagaga actttgcgaa agtctactat tcatgggaac taaacgacaa
     7201 gtccattgat aatcgcatag aaaacctgaa agcatatctg acagaggaac ttcaagctct
     7261 gaatgtcgat acagttcgta aagatattcc tgtatcgtct tctgtaaaag gatttcagat
     7321 atggacggta gaagcagatg gcgacaatca atttgatgta acctacagtg tagaccaact
     7381 cattacagag ggggaaaata caaagaccgt ccactctgct tatatagtga gtgtctatgt
     7441 agacagtact ggaaatatgg tcctcattaa gaatccgacc attactagca taccaaagaa
     7501 atcagactat aaaccaaaag ctattgaaag tgagggtacg gttgattcca ttacaaccaa
     7561 tgaaatcaat gagtttttaa cgacgttctt caagctctat cctacagcga cagccagtga
     7621 actttcctac tatgtgaatg acgggatatt aaaaccaatc ggaaaagagt acatctttca
     7681 agaactggta aatcctattt acaatcgtaa ggataatcaa gtcacggtat cgctgacagt
     7741 ggagtatatc gaccagcaga ccaaagcaac gcaggtatct caatttgatt tggtacttga
     7801 aaagaacggg agtaattgga agattataga ataacaaata ttggtacatt attacagcta
     7861 ttttgtaatc acgtactctc tttgataaaa aattggagat tcctttacaa atatgctctt
     7921 acgtgctatt atttaagtat ctatttaaaa ggagttaata aatatgcggc aaggtattct
     7981 taaataaact gtcaatttga tagtgggaac aaataattgg atgtcctttt ttaggagggc
     8041 ttagtttttt gcacccagtt taagaatacc tttatcatgt gattctaaag tatccggaga
     8101 atatctgtat gctttgtatg cctatggtta tgcataaaaa tcccagtgat aagagtattt
     8161 atcactggga tttttatgcc cttttgggct tttgaatgga ggaaaatcac atgaaaatta
     8221 ttaatattgg agttttagct catgttgatg c
//
LOCUS       DANKEF010000089         8015 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000089 DANKEF010000000
VERSION     DANKEF010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8015)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8015)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8015
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.090"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..1572)
                     /locus_tag="PWZ48_002564"
     CDS             complement(<1..1572)
                     /locus_tag="PWZ48_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2262181.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQR"
     gene            complement(1735..2328)
                     /locus_tag="PWZ48_002565"
     CDS             complement(1735..2328)
                     /locus_tag="PWZ48_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262182.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            complement(2329..2661)
                     /locus_tag="PWZ48_002566"
     CDS             complement(2329..2661)
                     /locus_tag="PWZ48_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262183.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            complement(2711..5242)
                     /locus_tag="PWZ48_002567"
     CDS             complement(2711..5242)
                     /locus_tag="PWZ48_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262184.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            complement(5239..7851)
                     /locus_tag="PWZ48_002568"
     CDS             complement(5239..7851)
                     /locus_tag="PWZ48_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HDL2262185.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
BASE COUNT         2207 a         1631 c         1282 g         2895 t
ORIGIN      
        1 tcgttgtctt cttccttgaa tcaatgcctg attccaaatc aacgtcatag ccatgtacat
       61 ttgacattgt tgtacccctc gatctaagcc tgtaatagag tcaatgtcaa aaaagaccaa
      121 ctgttgttgg tcaaggtgtc gaatcgtact atgcccatca aaaatcatgc catacgtctc
      181 acataaggat tctacgatgg attccatttc ttcttttcga ttctttttct cttgggaagc
      241 acctggtaat tccagttgtc ctaaaaagtc taaaaattct ttgaacgtcg gataagcttc
      301 tggatcttta cctgtgactt gcgtctccgt atttgaacgc gaatttgagg aagtaaagcc
      361 gcgtggtact gttccatact cttcataaaa acgatttaag taggtcttaa attctgcttt
      421 catcgttcct tttagctgag gactcactaa ctgtacctga tagaccactt tgtctaagtg
      481 tgtttgatag gacgctgctt cattgatctt caaatctttg actgatccat cgtcatataa
      541 actggatgct tcatcatacg tccgtgtggc aaatatctct aaaggattga tcattccgtc
      601 agatccgtct aaacgcacca taataccgtt atattccttt aagaaaggaa taaagtcttt
      661 tcccttttca aagccccaaa taaagtctcc tgcagctaaa tgggcttccg cgatcatttt
      721 aagaacggtc gatttaccag ctcccatcat tcctagaagc aaggtattag tagacaaccg
      781 tacactattt ttatacgtcg cattccagac aaatgggccg cctgtcatgg tttccccata
      841 ttgtgtccct cctggatctt ttagcgtttg gtggtggaac gggaaacctc cgccaatgtt
      901 taacgatggt agtgattgcc ctttgatatt tccgaaccat tcagcttgtt catcgaacat
      961 ggtaaacatc gattgccaat ctcgtttggt tttatgcaga taagtcaccg cacgataatc
     1021 catccctttt agattttcga tcagttcctg cgctcgttca tctacttctt gaatcgtgtt
     1081 tccgtaaaca tgtactctca ctttgacttg cttagaaatt tccccattag aagtcaattt
     1141 acgagcatat tccgctagtt ccaggtattc ttctgcgctt ttatttcggt cagtcgcttt
     1201 tcgttcatta tctgatcgat catttaattc atctaaggca cgattgattt gatcaatcac
     1261 tttttgcttt tcatctgtgc taacatcgag tgtggccact gtatgaggat tgttcatgag
     1321 attggccaac caaaaaagcg taggattttt tgtgtagtga taaactgtca cacatcgtga
     1381 ataaccatta ccatacgtca catattcttc atcaaaattt aacccacctt gtggcatcac
     1441 ttgttttaaa aaatctaaat catacccttt ggattttaaa atttctattt cccgatttcc
     1501 tagttgcttt aatggatcaa gtactttttg atcattttgt ggtgaaaaag ctgttttaat
     1561 tgcttctaac atgtacagtc acctcttctt gtttgattct ttacttggtt aaaagattgc
     1621 ccttttccca acttacccag gcttaaagtg tgaggaaatt ttctagtggt ctcttccttc
     1681 ggaacatctt tttctaacca tatcaacgga tatttcattc tctcttctcc attcctactc
     1741 atcattcata ttgttcattc gatacagaat cttgatcaat tcttttttgt agagtggttt
     1801 taaccctaaa atgccactag atcgcattaa ctgacggttt ctggtttgta aatccttgat
     1861 tgtctttcct tcaatgacga aaaagaagct taagtcagga cgttcttgtt ctgctcgttt
     1921 caattcctgg atttcttcga ctaatttctt ttcatatttt tttaatcgtt gtagatttct
     1981 tcctaaggac tgttccatac gatttttatc taataattgc cgcttttgca cgatttgttc
     2041 cgttagatcc gtctctgtgg tcaaactaac taacttaatt gttggcacat acgtacgcat
     2101 gacggtggtc aactgattca ttcgttctag tttctcttgt tcatttaatc cttccacgtc
     2161 tgtagaaact acacgataat aacgttgaaa atgatcttct ctagtaatta agactccctc
     2221 ttctgtacag gatttatacg gaaataagtc caagatagag cgctcttctg gttttccaaa
     2281 ctgcaaaatg gatttccttt caactgaatt ttgtcctttg actcgcattt attcatcctc
     2341 cttatacgta ccatgataat ttcggttagg atctaacgca tgataatggt tcttgtccgc
     2401 aaaaaaaata gactcgataa tcgcaaaaat ttgtcgttga cctgggttat tatagggtcg
     2461 ccaaacaatc actaacgcta tcgcaatcca taagccataa ttcaagagtg caaaaaagag
     2521 tggtaagtgg acaagattat tgaccatttt cgcccaaaaa aagcagccac caagcgttaa
     2581 tagatctaaa aagaaaaaag cgaaaaactt ccattctcca ctaaatcttg gagggttttt
     2641 catgtttata tatttatcca taattgattt ctccttttca tataaataaa aggcctaact
     2701 tgtaggcctt ttatggtttg atatcctgtt tgatatccct caatagttgt tctttcaatg
     2761 cattgttcct gtttttagtt tctcttgtca catttatttt tgttggtgca gtaaatgcca
     2821 catttgctgg cttgtaacct gtatgaacgt tttgaatagg ttttggtgca gcttctcttg
     2881 tcacaacatt ttgaacggac gcattccctg tctcaccact ttcagataaa ttgtaagaaa
     2941 cagttgtcag atttccattt tcaggcaaac gacttggttt catttgaaca gtacctgcag
     3001 ttgattggtg aacaaagctt tgatcattct ccttgaattg attcaaacga ctatctttca
     3061 ttggtaaatt ttgtgcctta ggtaaaccaa ttttcgttga tttttcagtt ccaacttcga
     3121 ttccattatc atttggcaga acgatggaat cctcatccgt ttgtacttct tgatttcgtt
     3181 ttggtaaatc tgaaatgcgt ttttcatttt cggagttaac agatctatac cgatattgtt
     3241 ctggatcagt ttttggtaac tgtccctcat tttctgcttt gtcttcttga tttacttttg
     3301 ataaatctga aattcgcttc tcattttcaa ggtcactgtt aggtttatag cgatattgtt
     3361 ctggatcagt ttttggtaac tgtccatttt ttccttcttc tgaatttctt tcttctatgg
     3421 aatttttctc ttcaatcgaa ttttcattag agtgcaacgc ttcttggtga tctttgactg
     3481 aaggaatcac tataccttct tgattttctt tttcggttgt tgtattagct tttactgctg
     3541 cattaacttg tccttcacca tacgcatctt ttagtccttc tttgacttct gaagcttttt
     3601 ctttttgttt ttctagtgct tcggatggat tcttcacagc ttcagccaat tctttagctc
     3661 tcgtgccgtc taatttctct tctaaggtgt ctttggcttt ttctgtaaga tcttcagctg
     3721 ttccttttaa cccacgttca ttgacgtaac caagaccttc tgcggcttta gaaacaccat
     3781 ttttcgcttt cccgaacatt tcggaaactg ttttcttact attaggtaca gttgtagctt
     3841 tatcgtctga ttgttcttcc ccgttatcag gggtaacatc ttttcgatgg ccaaatccat
     3901 ttttacgaat atcatttaaa tggcttaaac cactcttacc agcacttgca aggtttttag
     3961 ctccgctacc cacatttttc gcaatatttc ctaaggcaaa catgctaaga agagaattac
     4021 tgccgttttt cagactcgta tcaacaccga aaatcttcgt aaccgattga gagcctttga
     4081 atagcgcaat agttagcaca attaaagcaa caacatacaa gaatccattg atctttcctg
     4141 ccgcatgcaa atcaccgata cctgaaagca ttaatgtata gattcgtaat tctaaaatcg
     4201 taaacgccaa taacaagaag gattgagaaa tatcttctat cgcacttttc atccgttggc
     4261 ctgtctcaaa gtccgttgac gcggcaatca ctaaatagaa ttttttaaat accagctcaa
     4321 taatgcaagt aacgatcgta aataggataa ataaataggc caccgccaaa gaagattcac
     4381 cagctaagat aatacctggt ttactactgt atcgacgata ccctgaagta tagacatatt
     4441 ttgctaagcc ttcgtcacta atttgaacag gaatttcttt cccattatca tctagttcca
     4501 atcggtattg taacgctctt aaccgatcct tttgtaaatc tgataaacca ctcttatctt
     4561 tatccgccat ttctttagct tgtttaggag taataatccc attgatattc gctgttttaa
     4621 aatttttcgc tgttaaagca tttcttttgt ctgttggcag ggattctact ttattcgggt
     4681 cagttgctag taatgtactg acatcgatca cattattttg aataatttga aaagcaggtg
     4741 aacccgtttt atctgcttta tttgctgcag tgacgtctcc gtaaaagctt ttactgatat
     4801 ttaacccttc atcgattaaa gaactaccac caaaaatcaa gatcatggcc ataaataaat
     4861 taaccaagat atttttgact tttggtgggt gtttactcat aaaaaatttc aagcctaaat
     4921 aaattaatgt gacgacacaa aaaagagcca ataccttact aatcaaactt tgaaatagtg
     4981 catttacccc agcggcgttc aataagtctt ttaaatcaaa gctttgataa atggtttttt
     5041 ctagtaaagt attgagatta tataatcctt taatcaatcc ccacagaaaa gtgttccaaa
     5101 tccaactaaa tacatcttga tactgcagat aattaccata atcttgtaag actttcgtta
     5161 cggttttggg gtttgcatcc ccttctccga ataagcctga ccaccagtcg gctatgccag
     5221 aaaaaattcc tccaatgatc attctttcac caccttttca gattccaata acaaccgttt
     5281 cccaatcatt tcagaaattt ctttattttt gatcaacttt tcaacatatt cttgaagtcg
     5341 atctgctgct tgttcttcgt aaaattgaat taacaatttt ttagccgtct cattctcaag
     5401 agattgaacg attttataaa acatattttc ttcgtttttt tcttctaatt gatcatgcag
     5461 tccatgatag agatttctag catattcatc tggtaggaaa taatcataat caatttgtaa
     5521 agccttaaga tccaagtccg catgtgtaca atgtatgtct atatcattga tatcccacga
     5581 tggattgaaa tcttcaatat attgataagc ataaggaaga ttcgtttccc catgattaaa
     5641 aataggatac ggccgtattt tatgaccttt caaatcttgt cgttttaaag gagcgagtac
     5701 aatcgtttca ccttctaaaa aagtagaaat tctttcagga gttaagatac gatctgaatc
     5761 aacattgcga ttgatatgag tattcatacc taatgaattg atggtactgt tctcttgttc
     5821 aaccgtctta ttccccactt ttttagaaaa ttcttcgatg gtattatcat ctgtcgacat
     5881 aatataaata tgtgtctgac aattttcttt gataatcgct gcgttttgtt ctccatactt
     5941 cgcataaagt tgctgataag attgaataaa cagatcccac aacatgtttc ggccagctgt
     6001 gaccgtcatg attccttcca tattatccat tggtggcata ttcccaaatt catctaaaat
     6061 aaaatgaatc ctgcggtgac atttatctcc tttagtctta acacattgcg tagataattc
     6121 tttatataac tgactaagaa aaatactaac aatgatatgg ttagatggat cataatcagg
     6181 tgtaagcata aaaatagcca ttggtttatc ggtatagtgc atccgaatag actctactaa
     6241 taagttactt ttctcctcat ctaatgttag aacggcttct ttttctaatt ctggcaaatg
     6301 ggtttctcct cgttttttcc gataaacgat attaccctct tcgtctttca atactctttg
     6361 agaaaattca taccaagcca catattcttt tccttgatga tcgtatttta aacaaacttg
     6421 atcacctgca actaaatgac aatcaaaatt gatttcacaa aatcctttat agcctacttt
     6481 gacacgttct tcatgaaatt tctttccctt atgataaaat tctactacga ctcgttgatt
     6541 taaaagactt tcatgtaatt tcaaatctaa gtattttgga aacccaaccg atttataatc
     6601 aatcgttgat tgagaagtca tctttgcaat tttcgttaga gtaaatggat tcaatcccgt
     6661 gattgccgtt gataaaatat ttcccttcgc tttatcagat ccaattttcg ttgccccata
     6721 ttgttttttt gcaatgtttc caggtggcaa ctgattcata aattcatcta aggcattcaa
     6781 ctgtttgaat tcattgtctg gatctggata ataattaaaa ccgccaatct cagaaagaag
     6841 ctcaacgaga ttattcggtg tcaatttgga gtaatctcct gtttgatcag cttgttctaa
     6901 caacgcaaaa ataagcgcat tgacagcttt ctgcgcgttt tcgttaaagt atttgttatc
     6961 tgaagcgttg gcatcataat aaatcgtatt tgtgaaagta ttggttaact gtgttgcagt
     7021 atctatgtct cctcgtttcc agtatttcac gatgagtgcc aatggattat cactcattga
     7081 ttgcagtgga tcggtgatat taaaggcata tacatcatac cctctagctt ctagttcatc
     7141 tttactacca acatataatt ctgtttttgg atcattaacg accattgaac tttgtttttc
     7201 tgcacgagat aaattgtcaa tggtggctaa cacttcaatc tgacctttcc ctgatcgaga
     7261 ggtaccaaca atacacgtat taacagtatc tgtttgaatg taccaatatt ttttgtaatg
     7321 tgtgattgga atacctccat aacctggaaa agtagttttt gtttcaggta tttgcggata
     7381 aatctgttgg atttctttta aggtcattaa tcgattatcc cctttttgtc catctgctag
     7441 aggacgaaac cacatgtcca ttttcaatcc cataagtaca gctggaacca cacaaactgt
     7501 cgaaattgct aaacgtgtac ccaaactagt tgtaggagta aatggattcc aaaaaccagc
     7561 tgaatcaaaa tgaaagacct gtggcatttc ctgtaattga aacagattta ttccttgtaa
     7621 gcttccctta acaatttcca atcggttgaa aacttctggt actcttgtta ctaaatactc
     7681 taaagggagg tatacaagtc cactataaaa aatctttttc gaaatgcgtt tgtcatgttt
     7741 ttctggtagc tcttctcctg agggagtata gcgatagaaa tgagaaagat acctattctt
     7801 ttctaaattg aatcgtttgt cttttcgctt cgttgtttct ctaacacgca ttatttcacc
     7861 tcatttgatt ctgaactttc ggaagtgttt gattgtacag ttgaatcgct tgatccttct
     7921 ttagccttac tagaatcact ctctttgcta ttttccatgc ttgactctgt tgttgactga
     7981 ttggtacgat tcaataattc ttctaattgt tgttc
//
LOCUS       DANKEF010000090         8014 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000090 DANKEF010000000
VERSION     DANKEF010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.091"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            363..788
                     /gene="lacA"
                     /locus_tag="PWZ48_002569"
     CDS             363..788
                     /gene="lacA"
                     /locus_tag="PWZ48_002569"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HDL2262186.1"
                     /translation="MAIVVGADLKGTRLKDVVKNFLVEEGFEVIDVTKDGQDFVDVTL
                     AVASEVNKDEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     VGAEIVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMC"
     gene            805..1320
                     /gene="lacB"
                     /locus_tag="PWZ48_002570"
     CDS             805..1320
                     /gene="lacB"
                     /locus_tag="PWZ48_002570"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012027025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HDL2262187.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGHEVLDFGTYDHARTHYPI
                     YGKKVGEAVVSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSAIYAKEELNAN
                     VIGFGGKITGGLLMNDIIEAFIEAEYKPTEENKKLIAKIEHVETHNAHQADEDFFTEF
                     LERWDRGDYHD"
     gene            1331..2263
                     /gene="lacC"
                     /locus_tag="PWZ48_002571"
     CDS             1331..2263
                     /gene="lacC"
                     /locus_tag="PWZ48_002571"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HDL2262188.1"
                     /translation="MILTVTLNPSVDISYPLETLKIDTVNRVKDVSKTAGGKGLNVTR
                     VLYESGDKVTATGFLGGKIGEFIESELEQSPVSPAFYKISGNTRNCIAILHEGNQTEI
                     LEQGPTVSHEEAEGFLDHYSNLIKQSEVVTISGSLPSGLPNDYYEKLIQLASDEGVAV
                     VLDCSGAPLETVLKSSAKPTAIKPNNEELSQLLGKEVTKDIEELKDVLKESLFSGIEW
                     IVVSLGRNGAFAKHGDVFYKVDIPDIPVVNPVGSGDSTVAGIASALNSKKSDADLLKH
                     AMTLGMLNAQETMTGHVNMTNYETLNSQIGVKEV"
     gene            2266..3246
                     /gene="lacD"
                     /locus_tag="PWZ48_002572"
     CDS             2266..3246
                     /gene="lacD"
                     /locus_tag="PWZ48_002572"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HDL2262189.1"
                     /translation="MVLTEQKRKSLEKISDKNGVISALAFDQRGALKRLMAQYQDTEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDKEAGLLLAYEKTGYDTSSTK
                     RLPDCLDVWSAKRIKEQGADAVKFLLYYDVDSSDELNQQKQAYIERVGSECVAEDIPF
                     FLEILAYDEEISDAGSVEYAKVKPRKVIEAMKVFSDLRFNIDVLKVEVPVNVKYVEGF
                     ADGEVVYSKAEAADFFKAQEEATNLPYIYLSAGVSAKLFQETLQFAHDSGAKFNGVLC
                     GRATWAGSVEPYIKEGEKAAREWLRTTGFENIDELNKVLVKTASPWTDKV"
     gene            3274..3591
                     /locus_tag="PWZ48_002573"
     CDS             3274..3591
                     /locus_tag="PWZ48_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011669100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDL2262190.1"
                     /translation="MNREEMTLLGFEIVAYAGDARSKLLEALKAAENGDFAKADSLVV
                     EAGSCIAEAHNSQTAMLAREASGEELPYSVTMMHGQDHLMTTVLLKDVIHHLIELYKR
                     GAK"
     gene            3591..5297
                     /locus_tag="PWZ48_002574"
     CDS             3591..5297
                     /locus_tag="PWZ48_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2262191.1"
                     /translation="MHKLIELIEKGKPFFEKISRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAYVPNAWGFHWSKDIETFLMTPYSYSMGILAFFVGGTTAKALTDSMNRDLPATNQ
                     INFLSTMLASMVGFLLMAAEPAKEGGFLTAFTGTKGLLTAFIAAFVTVNVYKVCVKNN
                     VTIRMPEEVPPNISQVFKDLIPFTVAVVLLYGFELIVKGTLGVTVAESIGTVLAPLFS
                     AADGYLGITLIFGAYAFFWFVGIHGPSIVEPAIAAITYANIDANLHLIQAGQHADKVI
                     TSGTQMFIVTMGGTGATLIVPFLFMWICKSERNRAIGRASVVPTFFGVNEPILFGAPI
                     VLNPIFFVPFIFAPIVNVWIFKFFVDTLNMNSFSANLPWVTPGPLGIVLGTNFQVLSF
                     ILAGLLVVVDTIIYYPFVKVYDEQILEEERSGKTNDALKEKVAANFNTAKADAVLGKA
                     GVAKEDVAANNNITKETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGSYGAHR
                     EMLPEFDLVILAPQVASNFDDMKAETDKLGIKLAKTEGAQYIKLTRDGQGALAFVQQQ
                     FD"
     gene            5439..6845
                     /gene="lacG"
                     /locus_tag="PWZ48_002575"
     CDS             5439..6845
                     /gene="lacG"
                     /locus_tag="PWZ48_002575"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HDL2262192.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYHKYPVDLELAEKYGVNGIRISIAWSRIFPTGYGEVNEKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEVLHSDGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHARAVKLYKDKGYKGEIGVVHAL
                     PTKYPYDPENPADVRAAELEDIIHNKFILDATYLGHYSDKTMEGVNHILAENGGELDL
                     RDEDFQALEAAKDLNDFLGINYYMSDWMQAFDGETEIIHNGKGEKGSSKYQIKGVGRR
                     IAPDYVPRTDWDWIIYPEGLYDQIMRVKNDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKKHLEVLSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFDTQERY
                     PKKSAHWYKKVAETQVIE"
     gene            6868..7140
                     /locus_tag="PWZ48_002576"
     CDS             6868..7140
                     /locus_tag="PWZ48_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HDL2262193.1"
                     /translation="MLSDTLEKVQHLFRPGFEKVYIVSFEDCPMIPELEATPLLKCGK
                     WYVSTGKEWICHSDLELSAFEWEFLQSLDVEIRETIHFEVNYLPFQ"
     gene            7161..>8014
                     /locus_tag="PWZ48_002577"
     CDS             7161..>8014
                     /locus_tag="PWZ48_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002889672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDL2262194.1"
                     /translation="MTIELKNEYLTVQFKTLGGQLTSIKDKDGIEYLWQADPNYWNGQ
                     APILFPICGSLRNDWAIYRPQERPFFTGLIRRHGFVRKEEFILEEVNDNSVTFSIKPN
                     AEMLDNYLYQFELRVVYTLNGKSIRTEFQVTNLEMEKTMPYFIGAHPAFNSPLVEGEN
                     YEDYYIEFSEVESCSIPKSFPETGLLDLQDRTPFLENQKILDLDYSLFSHDAITLDQL
                     KSRSVTLRSRKSGKGLRVDFDDFPNLILWSTTNKSPFIALEPWSGLSTSLEEGNFLED
                     KRQVTKISP"
BASE COUNT         2535 a         1370 c         1674 g         2435 t
ORIGIN      
        1 aataagaaca gtggattgac gtatattagc ttaggtcaga ttttgtataa gacgaaaata
       61 aagtaggacc tcttaatcag taagttatag aaagtaaaag acttttgtaa tacttgaata
      121 gatatttcac gtccattttg tgatggatta aatgaacaaa aatgaacaat aatttaacgg
      181 tgttatctat tttttaaaaa aacaaataaa aaaacaaaaa ataaacaaaa aagtgttgct
      241 ttttgtttga atgcttgata ttatataaac aaagaaatga tgaaaacgtt ttttcgaaca
      301 ttttgcaaaa tgttttctac ttctacgtag catttctttc taaaatttag gaggtggtca
      361 aaatggctat tgttgttggt gcagatctca aaggtacgag attaaaagat gttgtgaaaa
      421 attttcttgt agaagaaggt tttgaggtaa ttgatgtgac taaggacgga caagattttg
      481 ttgatgttac cttggcagtt gcctctgaag taaataaaga tgagcaaaac ttaggtattg
      541 taattgatgc ttatggagct ggtccattca tggtagcaac taaaattaaa ggtatggttg
      601 cagcagaagt ttcagatgaa cgctcagctt atatgacacg tggacataac aattcacgaa
      661 tgattacggt tggcgctgaa atcgtcggtg atgagcttgc taaaaacatc gctaaaggtt
      721 ttgtcaatgg taaatatgat ggcggacgtc atcaaatccg agtagatatg cttaacaaaa
      781 tgtgttaagg aaaggagtta aggaatgaga atcgcaattg gatgtgacca cattgtcaca
      841 gatgaaaaaa tggcagtatc agaattcttg aaatcaaaag gacatgaggt ccttgatttc
      901 gggacatatg accacgcacg tactcactac cctatctatg gtaaaaaagt cggtgaagca
      961 gtagtaagtg gtcaagcaga cttgggagta tgtatctgtg gtacaggtgt cggcattaac
     1021 aatgcagtca ataaagttcc aggtgttcgc tcagcgttgg ttcgtgatat gacatcagct
     1081 atatatgcaa aagaagagtt aaatgcgaat gttatcggtt tcggtggaaa gattactggt
     1141 ggtcttctta tgaatgacat cattgaagct ttcattgaag ctgagtacaa accaacagaa
     1201 gaaaataaaa aattgattgc aaaaatcgaa cacgttgaaa cacataatgc acatcaagca
     1261 gatgaagatt tctttacaga attccttgaa agatgggacc gtggtgacta ccacgattaa
     1321 ggtataacaa atgattctga cagtcacact aaatccttca gtagacatct cttatcctct
     1381 agaaacattg aaaattgata cagtgaatag ggtgaaagat gttagtaaga cagctggtgg
     1441 aaaaggctta aatgttacta gagtcttgta tgaatctggt gataaagtta ctgctacagg
     1501 tttcttaggt ggtaaaattg gtgaatttat tgaaagcgaa ttagaacaat ctcctgttag
     1561 tccagctttt tataaaatct caggaaacac tagaaactgt attgctatct tacacgaggg
     1621 taaccaaaca gaaattttgg aacaaggacc aacagtttct catgaagaag cagaaggttt
     1681 tcttgaccac tatagcaatc ttattaaaca atcagaagtt gtgacaattt caggaagttt
     1741 accttcagga cttcccaacg attattatga aaaacttatc caacttgctt cggatgaagg
     1801 agtagcagtc gttttggatt gttcaggtgc acctcttgaa acagttctaa aatcgtcggc
     1861 taaacctact gccatcaaac ctaataatga ggaactttca cagttgcttg gaaaagaagt
     1921 aacaaaagat attgaagaac tcaaagacgt tcttaaagag tcacttttta gtggtatcga
     1981 atggatagtg gtctcattag gtcgaaacgg tgcctttgca aaacatggtg atgttttcta
     2041 taaggtagat attcctgata tcccagttgt taatccagtc ggatcagggg actcaacggt
     2101 agctggtatt gcatcagctt taaatagtaa aaaaagtgac gctgacttat taaaacatgc
     2161 gatgacattg ggtatgttaa atgctcaaga aacaatgaca gggcatgtga atatgactaa
     2221 ttacgaaaca ctaaactcac aaattggagt aaaagaggta taaaaatggt acttacagaa
     2281 cagaaacgca aatctttgga aaaaatttca gataaaaacg gtgttatctc agctttggca
     2341 tttgaccaac gtggtgcttt gaaacgtttg atggcacagt atcaagatac agaaccaact
     2401 gtagctcaaa tggaagaact aaaggttttg gttgctgatg aacttacaaa atacgcttct
     2461 tcaatgttgc ttgaccctga atatggactt ccagcaacaa aagcattgga taaagaagca
     2521 ggtcttcttc ttgcctatga aaaaacaggt tatgatacat caagtacaaa acgtttgcct
     2581 gactgcttgg atgtttggtc tgcaaaacgt atcaaagagc aaggcgctga tgccgttaag
     2641 ttcttgcttt actatgatgt agacagctca gatgaactta accaacaaaa acaagcttat
     2701 attgaacgtg ttggttctga atgtgtcgct gaagatattc ctttcttctt ggaaatcctt
     2761 gcttatgatg aagaaatctc agatgcaggt tctgttgaat atgcaaaagt gaaacctcgc
     2821 aaagtcattg aagcgatgaa agttttctct gatctacgct ttaacattga tgttcttaaa
     2881 gtagaagtac cagttaacgt taaatatgtc gaaggttttg ctgatggtga agtggtttac
     2941 agtaaagctg aagctgctga cttctttaaa gcacaagaag aagcgactaa ccttccatac
     3001 atctacctaa gtgcaggtgt atctgctaaa ttgttccaag aaacattgca atttgctcac
     3061 gactcaggtg ccaagtttaa tggtgtgctt tgtggacgtg ctacttgggc aggatctgta
     3121 gaaccttaca tcaaagaagg tgaaaaagca gcacgtgaat ggttgcgtac tactggattc
     3181 gaaaatattg atgaacttaa caaagttctt gttaaaacag ctagtccatg gactgataaa
     3241 gtatagtaaa aacataaaac ggaggatatt gttatgaaca gagaagagat gactctctta
     3301 gggtttgaaa ttgttgctta tgctggagat gctcgctcta aacttttaga agcgcttaaa
     3361 gcggctgaaa atggtgattt cgctaaggca gatagtcttg tagtagaagc aggaagctgt
     3421 attgcagagg ctcacaattc tcagacagct atgttggctc gagaagcttc tggggaggaa
     3481 cttccataca gcgttaccat gatgcatggt caggaccact taatgactac tgtcttatta
     3541 aaagatgtga ttcatcacct catcgaactt tataaaagag gagcaaaata atgcataaac
     3601 tcattgaact tattgagaaa gggaaaccgt tctttgagaa aatctctcga aacatctatc
     3661 ttcgtgctat tcgtgatgga tttatcgctg gtatgccagt catccttttc tcatctatct
     3721 ttatccttat tgcctatgtc ccaaatgctt ggggattcca ctggtcaaaa gacattgaga
     3781 ccttcttgat gactccttat agctattcga tgggtatcct agcattcttt gttggtggta
     3841 ctaccgctaa agctttgacg gactcaatga accgtgacct acctgcgacc aatcagatta
     3901 acttcttatc tacgatgcta gcgtctatgg tagggttcct tttgatggca gctgagcctg
     3961 caaaagaagg aggcttcttg acagccttca cgggaacaaa aggtcttttg acagcattta
     4021 ttgcagcctt tgtgacagtt aatgtttaca aggtctgtgt gaaaaataat gttactattc
     4081 gtatgcctga agaggttcca ccaaatatct ctcaagtatt taaagacttg attccattca
     4141 ctgtagcggt tgttctcctt tatggatttg aactcattgt taagggaact cttggtgtaa
     4201 ccgttgcaga atcaatcggt accgttcttg ctcctctctt ctcagctgca gatggttacc
     4261 ttggaattac acttatcttt ggtgcttatg ccttcttctg gtttgttggt atccacggtc
     4321 cttcaattgt cgaaccagca atcgctgcta tcacttatgc caatatcgat gccaacttgc
     4381 atcttatcca agctggacaa catgcagata aagttatcac ttctggtact caaatgttta
     4441 ttgttaccat gggtggaaca ggagctacat tgattgttcc attcttgttc atgtggattt
     4501 gtaaatcaga acgtaaccgt gccatcggac gtgcctcagt tgttccaaca ttctttgggg
     4561 ttaatgagcc aatcttattt ggtgcaccaa tcgtattgaa tccgattttc tttgtaccgt
     4621 tcattttcgc tccgattgtc aacgtttgga tctttaaatt ctttgttgac acattgaaca
     4681 tgaactcatt ctctgccaac cttccatggg ttactcctgg tccgttggga attgtactcg
     4741 gtactaactt ccaagttcta tcatttatct tagccggact cttggtagtt gttgatacta
     4801 tcatttatta cccatttgtt aaggtatacg atgaacaaat tcttgaagaa gaacgttctg
     4861 gtaaaacaaa cgatgctctt aaagaaaaag tagctgcaaa cttcaatact gctaaagcag
     4921 atgccgttct tggaaaagca ggcgttgcta aagaagatgt agcagcaaac aataatatca
     4981 caaaagaaac taatgtcctt gttctttgtg caggtggagg tacaagtggc ttgcttgcca
     5041 atgccttgaa caaagcagca gcagaataca atgttccagt caaagcggca gcgggtagtt
     5101 acggtgctca ccgtgaaatg ttgccagagt ttgacttggt tatcttggct ccacaggtcg
     5161 cttcaaactt cgatgatatg aaggctgaaa cggataaatt gggcattaaa cttgcaaaaa
     5221 ctgaaggagc tcaatatatc aaattgacac gtgatggaca aggtgctctt gcatttgttc
     5281 aacaacagtt tgattaataa atttgtaaaa gttatttgag acgagattat tctttcagtt
     5341 ccttataata ttgttaacca tatcattgca tttcatttcc ttggtttccg gaactcagaa
     5401 tatcgtctca aatgcttttt ttgaaaggat ttatagttat gactaaaaca cttcctaaag
     5461 attttatttt tggtggtgct acagctgctt atcaagcaga aggtgcgact cacacggatg
     5521 gtaaaggccc agttgcatgg gataagtatc ttgaagacaa ttattggtac acagcagagc
     5581 ctgcaagtga tttttaccat aaatatcctg ttgatttaga attagctgaa aaatatggcg
     5641 ttaatggtat tcgtatttca attgcctggt ctcgtatttt cccaactggc tatggcgaag
     5701 taaatgaaaa aggtgttgaa ttctaccata atctttttgc cgagtgccac aaacgtcatg
     5761 ttgagccatt cgtgactttg caccactttg acacaccaga agttcttcac tcagatggag
     5821 atttcttaaa ccgcgaaaat atagaacact ttgtagatta tgccgctttc tgttttgaag
     5881 agttcccaga agtaaactac tggacaactt tcaatgaaat tggcccaatt ggagatggtc
     5941 aatacttggt tggtaaattc cctccaggca ttcaatatga tcttgccaaa gtcttccaat
     6001 cacaccacaa tatgatggtt tctcatgcac gtgctgtgaa attgtataaa gataaaggtt
     6061 ataaaggcga aattggtgta gtacatgcgt tgccaaccaa atacccttat gatccagaaa
     6121 atccagcaga tgttcgtgcc gctgagttag aagatatcat ccataacaaa tttatcttgg
     6181 atgcaaccta ccttggacac tattcagata aaacgatgga aggggtcaac cacatcctag
     6241 ctgaaaatgg tggagaactt gatcttcgtg atgaagactt ccaagcactt gaagcagcta
     6301 aagatttgaa cgatttcctt ggtatcaact actacatgag tgattggatg caagcctttg
     6361 atggtgagac tgaaatcatt cacaatggta agggtgaaaa aggaagttct aagtaccaga
     6421 ttaaaggtgt tggtcgccga atcgcaccag attatgtgcc tagaacggat tgggactgga
     6481 ttatttatcc tgaaggcttg tatgaccaaa tcatgcgagt gaaaaatgat tatccaaact
     6541 acaagaagat ttacatcact gaaaacggtc ttggatacaa agatgagttt gtagataata
     6601 ccgtttatga tgatggtcgt attgattacg tgaagaaaca cttggaagtt ttatcagacg
     6661 caattgcgga tggtgcaaat gttaaaggtt acttcatctg gtcacttatg gatgtcttct
     6721 catggtcaaa cggatacgaa aaacgttatg gactattcta tgtagacttt gacacgcaag
     6781 aacgctatcc taagaaatca gctcattggt ataagaaagt agcagaaact caagtgatag
     6841 agtaatatta cttgaaaatg taaaaaattg ttatccgata ctttagaaaa agtacaacac
     6901 ttatttagac caggctttga aaaggtatat attgttagtt tcgaagattg tcctatgatt
     6961 cctgagcttg aagctacccc gttgctaaaa tgtggtaaat ggtatgtttc aactggaaaa
     7021 gagtggattt gccattctga tttagagtta tcagcatttg aatgggaatt tttacaatca
     7081 ctcgatgtag aaatacgtga gactattcac ttcgaggtaa attatttgcc tttccaataa
     7141 taaaaagaaa ggaattagtt gtgactatcg aattaaaaaa cgagtatctt actgttcagt
     7201 ttaaaacact tggtggtcaa ctgacttcga ttaaagacaa ggatgggata gagtatctct
     7261 ggcaagctga tccaaattat tggaatggac aggcgcctat tctatttcct atctgtggta
     7321 gtttacgaaa tgattgggct atttatagac ctcaagaaag accttttttt acaggtctta
     7381 ttcgtagaca tggttttgtt cgaaaagaag aatttattct ggaagaagtc aatgataata
     7441 gcgtgacctt tagtattaaa ccaaatgctg agatgcttga taattaccta tatcagtttg
     7501 aactgagagt tgtttatacc ttgaatggta aatcaattag aaccgaattt caggtaacga
     7561 acttggaaat ggaaaaaaca atgccttact ttattggagc ccatccagca ttcaatagtc
     7621 ctttagtaga aggtgaaaac tatgaggatt attatattga atttagtgag gttgagtctt
     7681 gctcgatacc aaagagtttt cctgagacag gattattaga tttgcaagat cggacacctt
     7741 ttttggaaaa tcaaaaaatt ctcgatttgg actactcact tttttcccat gatgccatta
     7801 cacttgacca gctcaagtct cgtagcgtaa cgcttcggtc aaggaagtct ggtaaaggtc
     7861 ttcgagtaga ttttgatgat tttccgaact tgattctgtg gtctacaact aataaaagtc
     7921 cattcattgc tttggaacct tggagtggtc tctccacttc acttgaagaa ggtaatttct
     7981 tagaagataa aagacaggta acgaaaattt cacc
//
LOCUS       DANKEF010000091         7741 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.092, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000091 DANKEF010000000
VERSION     DANKEF010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.092"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(32..1180)
                     /gene="nagA"
                     /locus_tag="PWZ48_002578"
     CDS             complement(32..1180)
                     /gene="nagA"
                     /locus_tag="PWZ48_002578"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDL2262195.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1256..2092)
                     /locus_tag="PWZ48_002579"
     CDS             complement(1256..2092)
                     /locus_tag="PWZ48_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2262196.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSQDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2276..3493)
                     /locus_tag="PWZ48_002580"
     CDS             complement(2276..3493)
                     /locus_tag="PWZ48_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HDL2262197.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     ITSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3642..3821)
                     /locus_tag="PWZ48_002581"
     CDS             complement(3642..3821)
                     /locus_tag="PWZ48_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262198.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3876..3959
                     /locus_tag="PWZ48_002582"
     tRNA            3876..3959
                     /locus_tag="PWZ48_002582"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3910..3912,aa:Leu,seq:gag)
     gene            complement(4199..5569)
                     /locus_tag="PWZ48_002583"
     CDS             complement(4199..5569)
                     /locus_tag="PWZ48_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262199.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSLQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(6284..6574)
                     /locus_tag="PWZ48_002584"
     CDS             complement(6284..6574)
                     /locus_tag="PWZ48_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262200.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(6782..6985)
                     /locus_tag="PWZ48_002585"
     CDS             complement(6782..6985)
                     /locus_tag="PWZ48_002585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262201.1"
                     /translation="MNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLE
                     KAKDEVEDTADKSQQIVEEHLRK"
     gene            complement(7082..7474)
                     /locus_tag="PWZ48_002586"
     CDS             complement(7082..7474)
                     /locus_tag="PWZ48_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase"
                     /protein_id="HDL2262202.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEIN"
BASE COUNT         2366 a         1529 c         1182 g         2664 t
ORIGIN      
        1 aagtttagtt ttaaggttaa cttaaattat attatgcgtg gaaacgttcc acgccatcta
       61 gataagttgc ttccaatgtc atatcagggt tcaatacgat aaagtctgca tcgcgtcctt
      121 gagcgatttt tccacatgta tcatcgattt tacagctgat tgctggaatc agactagcca
      181 tcatgactgc ttgttctggt gttgctaatc cccaatctac aacattttta attgcttctt
      241 tcaatttcaa tatacttcca gctaagttgc cagattctag acgtgctgtt ccttctttta
      301 ctactactgg aaattcgcct aaattgtaat ttccatcagg catcccaccg gccatcatac
      361 agtctgtaat taatgcgaca tgatcatgtc ctgctttttc cattaggatt cccgcagcat
      421 ttggatgtac atgatggcca tcacaaatca attcagaaaa gacatgttct aaagataata
      481 acgcacccac catacctggt tcgcgatgat tcaatccacg cataccattg aaagcatgga
      541 caaatacact ggcgccagct tcaacagctt tttgcgcttc ttctagcgta gcgttactgt
      601 gacctagaga aacaactact ccttcttcag tcacttgttt gacaaactct tctacacctt
      661 gacgttccgg tgctaaagca atttttttga ttaaaccgcc tgaagcttct tgccattcat
      721 ggaaagtatc caaatcagga tccccgaaat aacttgggtt ttgtgcccct ttgtgttctt
      781 ctgtgaagaa aggaccttca aaatagattc ctttgatttt agctcctggt gcttcctgat
      841 acattttgcc gattgtctca gctacatctt ttaaacgttc tttgcttgac gtcaatgttg
      901 ttggtaaaaa tgaagtcact ccacaagata acagaccttc agacatcact ttgatgcctt
      961 ctgcatcatt atccattaca tcatgattca tatagccgtg aatatgtgta tctaccaaac
     1021 caggagcaat ccatttgccg ctttggtcaa tgatgttgac ttctccctca ggtagttctt
     1081 tggtgtattc accaaaaagt ccatcggtaa tttcaagata gcctgcatgt ttgacacctg
     1141 acgctaaaaa gaatttgtca gcatagataa aagttctcat tctattctct ccctttgttt
     1201 ttctacctct aaagtactct tatttagaga agaagtcaag aatggtctac accatttaca
     1261 aattttttga aatttgtttc acagcttgtg ctaagccttc atagaatgct gctctttctg
     1321 gttcttcttt acgcttgtat attaagcttt taagcacaaa taaatcattg aggtaatagt
     1381 agctattctt ctcttgagcc caatagattc cttgattcac ctgttctaac gcttcgtcga
     1441 tttcattttg atggaataag aaatttccat aattataaaa aatttgcgaa aattcacttt
     1501 ttgatcgttc gtcagctgat tggtgcaaca gttgaatgct tcgactcaaa taatagcgtg
     1561 cttcagccgt ttttcctgca acaccatata tttttccaat gcaactgatc agttgtattt
     1621 ctgtgtcaga aacatggatt ctttcttttt ggctagagta gcttaatgct tctttcaaat
     1681 aataaaggga ctgagagata ttttgacgca acgtgaattc gcaaacaccg agataataat
     1741 aatagcgttg aaagtcagtt gcttgataca gattttcaga aatatcagaa gattttagta
     1801 cttgttctaa cttccgataa tcacgttttt ggaaacaact atccaacagt tcaaataatt
     1861 tatcgtttgt ccgagtagtt tttcgagaga gattcatcac atgatcaatc gttaccccta
     1921 gccgttgaca aatctgcatc atcaccaata cattcggcag ttcctcatta ttttcgatcc
     1981 gacttaatac actttgtgaa caaatatctt gcgaaagcat tttttgcgtc attttttgtt
     2041 cttttcgaat ctctttaatg atcgtgccaa aggactcgat taacgtttcc ataagtatcc
     2101 tccccaaaat atgcgtattc gcattctatt cgtattttac tttaggaagt cgcaaacttc
     2161 aagaaaagac ttgcaactaa ataatatact ttagttattt aagaaaactc cactagaaag
     2221 agagcctcag tgtgtttgtc tgtggccctc ctactgaaaa atttggtttt taaaatcatt
     2281 tttggcgata ggttgctagg aattctctca ttttcttagc tgtttgtttc aaatcatcca
     2341 ttttaggaag ataaacgatt cgaaaatgat ctggctggtt ccaattaaat ccaccaccat
     2401 gtacaagtag aatatgatgt tcatgtaaaa agtccaaaac gaatttttca tcatccaaaa
     2461 tattgaatcg ctttgtatca atttttggga atatataaaa agcagctttt ggttttacgg
     2521 ctgaaagacc aggaatatca ttgattgcat tatagatata ttctctttgt tcatagatcc
     2581 ttccgcctgg caggagcagc tcatcgacac tttgataacc gcctagcgcg gtttggataa
     2641 tttgctgaga caatacattg gaacacaaac gcatggagga caacatattc agcccttcta
     2701 tatagccttt aacgtgtgac ttatctccac ttaggaccat ccagcctact cgaaatcctg
     2761 ctacacgatg agatttggat agaccattca gcgtaacaac gaacaaatca ggcgctaatg
     2821 tagcaatcgg aatatgttct agaccatcca tcactaagcg atcatagatt tcgtcggaat
     2881 aaataatcag gttattttgt cgagctactt caacgatttc ttctaataat tcttttggat
     2941 aaagtgcccc agtaggatta ttaggattaa tgataacaat agcttttgtc cgactcgtta
     3001 tttttgatgt gatatcatca atatcaggat accattctgc ctcttcatcg caaatataat
     3061 gaacaggtgt gcctccagct aatgcaatag aagccgtcca caacggatag tccggcattg
     3121 gaaccagtac ttcatctcca ttgtccagca acccctgcat acacatcgtg atcaattcgc
     3181 tcactccgtt gccggtgtag atatcattga tcgtcacatt cggaaaatgt ttcagttggc
     3241 aatattgctc aattgctttt ctagcagaaa agatcccctt ggaatctgag tagccttctg
     3301 agtcacgggc attcatgatc aaatcacgaa tgacttcgtt tggtgcttca aatccaaaag
     3361 gtgcgggatt tcccgtgttc aattttaaaa tactaatgcc ctcttcttgc atccgctccg
     3421 cttcttctag aacgggaccg cgaacatcgt aactgacacc ttctaatttg ttggattttt
     3481 caaattctct catcacttag ccaatccctc atttcaaaaa gttagaactt tccaaatttt
     3541 ttgttattta ctatttcaat atcatactcc atcctagaaa attttgccac ttatttagga
     3601 aaaacgtaag tattaaaaaa acacaaacaa gcgttaagaa cttaattgtc atgatagtca
     3661 ttccatagat tcgctgccca atccattgca attccacaaa tgattcctag cataaaacca
     3721 agcaaaaaac gtccgaatgc aagtccaatc aatacaccta agatcattcc cagtcctgaa
     3781 aattctccgt catatttctc catcttaata agtcctccca tttttgagct tgacaactat
     3841 gtaaaacctc gctatcatta ttgtgttatt attacgcaga tgtgttggaa ttggcagaca
     3901 agcatgattg agggtcatgt gagcgttagc tcgtgtgggt tcgagtccca tcatctgcac
     3961 tacaactcgt gaagttaatt ttgcttcacg agtttttttg tttgaacgtt tctttatgcc
     4021 attatataca tatcactgta tcctttacaa aaagaacgta caatcatgta aacagcctga
     4081 ggtgagtata tgaaggtcgg aaacaagcta ctcgtagaaa cttgcaagtg ttaaaacaat
     4141 taatcgaaaa acgagtaaaa tccctcacct aatactagag cgagggattt tttgtatatt
     4201 atctgtttat caccgttatt ataaaggtac aaataccaag cacggttaat ccaaattgca
     4261 taaattcatt tttttgtaaa ctcatgcttt tcattagctc tttaactgcc attagtacta
     4321 agatggcgat aacaacataa agtatatagt acgtccgcat tccggaagcc catactgtgg
     4381 gagaaaatcc cataattaac cttgaacaaa atcccattat catcaaaata actaaaaaaa
     4441 tagaagtttt cttattgtca aaacttaaat aaattccaac aattaaacat agtaataatg
     4501 ccaagactac taaagtagct atccaagtac ctggataaaa aagtgacagt ttcgttccta
     4561 actctgtaaa gagattattc aggttactac tactccaaat cattgccctt ttatttccat
     4621 tgacgtaagt aaagctttga cccattgtat taccaaaata aataatcagg tttaagaaaa
     4681 acggtaatgc agtcaatata cgcacataat agttttgaca tttcctatat gttagaagga
     4741 aaattaagaa aaatagcaat aaaaataaga tattcatgtc taaaaaaaac ggctttccaa
     4801 atgatgaaat acctaaatct aacttattaa gaaagttgaa atttttatat tcaggaaacc
     4861 atttattaat ttcatgtgca gcacgtaatg cattccctgg agttgtcaaa gagaaaatta
     4921 attctgctaa gcttattatt gaaaatactg ataacttata attatatcgt ccattgacaa
     4981 tcaaatataa actaactata cttgttagaa caaaaaaaca ggcgttgact tgttcttgat
     5041 tagttaaaaa tatcaataaa ggaatactta cccaatatat ttttctattt atttcttctc
     5101 ctcttaagag ttgaaaaaac ggataaaaag ttagtaaact aaatgctact ggccactgat
     5161 agttcaaggt ggttgctctc caaccggttt cccccattac tgtcaatggt attaaacagt
     5221 atatagagca aaccaataat aaatttttaa catttatttt ctcaaaaaca tacttgcaaa
     5281 gtaatactac aattcctaac ataatcgtag aatttaataa tttccatagc ataaagtgtt
     5341 tagaaaagaa cattaagaat agttctataa taattcttga actccatgtc ttataatgtt
     5401 ctatcgaagc acccaaatag ccaagttcat ctaatctact tgcataaact acgtcatccg
     5461 ctctcatagt cggcatatac atattaaaga gtaaaaagat accccaaatc cctattagta
     5521 acaagatcag ttctttgtta ttcgttattt ttctattatg tttgttcatt tttatactcc
     5581 ttcttatatt tcctaataga gtataacata gctttaagca ttacttaaag ataacttaaa
     5641 agttatcttt taaatactta attgttaaat accttatttg atgcaaaaga aatctacgat
     5701 agtcaacaaa aaaaattttc attatatcca gaaaaatttt tcttagttaa ggacctatgg
     5761 attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca tattgctttt
     5821 aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt aggtttaact
     5881 gtcgaaccag gacgttctcg tgtgaaagac gaggcttctt caatttactt ttacgactat
     5941 gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa atcctacaat
     6001 tccactacct gagactagta atgactagtc tctttttatg gcctctgatc cccccattga
     6061 gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat ccctcaaatg
     6121 agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta tatatagatt
     6181 gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgaa tccatctttc
     6241 caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt attctttcat
     6301 cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca attttgggtt
     6361 tccctaacac atgataggat ctcacaacaa attgctttct attattataa taacctgtaa
     6421 cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg gaagcaatca
     6481 aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact tgcaaagtac
     6541 ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata ccattatacg
     6601 aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt tttgtctatt
     6661 acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta ctaaaaaaaa
     6721 gaggcagcag tatagattac tgttgcttct ttactaattt aggtttactt gttaagaaag
     6781 attatttacg caaatgttct tcaacaattt gctgagattt atcagctgta tcttccactt
     6841 catcttttgc tttttctaac ttttgaatat attgtttcaa gcttgagttt tcagcagttg
     6901 cttcgtctaa tttcttttgt gtatctgtta actgagtttc tacatttgtt ttttcttgtg
     6961 tcagtgttgc gatttgttga ttcaaaccat ttacagtatt ttgtaattct gaaagttttg
     7021 cttgtgcttc ttgtagtctt gctgtccaat ctgcattcag atgattgatt tcatcatttt
     7081 tttagttgat ctcatctagt ttgacattga tttcattttg cttgctttgt agttgtgcat
     7141 ccttgctgac taattgactt tctaagtttt gtttttctgc tgccgattga tttttttggt
     7201 tttctaattc atttactttt gtttggtatt cttggacatc tgcctttgaa tcttgcaatg
     7261 tctgttcttt ttgtgctagt cgagcggtaa gatctgtaat cgcttgctct ttatctgtca
     7321 atgtatgatc aagctgtgtc agattctcat tgattgtatc aatatctgtc tctccacccc
     7381 acaaaataat ttcatttgcg taccgcgtgc ctgtgtagcc aattagacat agaaatgcaa
     7441 cgacaatccc accagtaata aatttctttt tcatttgcat agctccttaa tccaatactt
     7501 ttacatacta aaagtaagtg atctcaaaca caatgtcaag cgtttttaaa ttacatatgt
     7561 aattttaata taaatactat caaatactca tttaatcctc cttttttatc tatttaaaaa
     7621 atctttttta tagagaaaaa tgcaggttaa tactttctta aataaatatt tatggtgaac
     7681 aaaaacaaaa aagcaagttt tattaaacgt aacccttttc atttcataga ttactgtatt
     7741 t
//
LOCUS       DANKEF010000092         7740 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000092 DANKEF010000000
VERSION     DANKEF010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.093"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(690..1025)
                     /locus_tag="PWZ48_002587"
                     /pseudo
     CDS             complement(690..1025)
                     /locus_tag="PWZ48_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313007.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1122..1574)
                     /locus_tag="PWZ48_002588"
     CDS             complement(1122..1574)
                     /locus_tag="PWZ48_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262203.1"
                     /translation="MKSLEQEHMAFKQAMFKENIYLNHNYIRVSKACSPVLNMLGGGN
                     GLYHLLFVDVCWLIFLPDELVIVNEKITSKNEVFNYSLTRINYKEITKFSLEKVPFWG
                     EFCLKIKCNWKRMYFYIDGDDALTFGKTTFSSFNFQFLLKNNFYGLLK"
     gene            complement(1581..2129)
                     /locus_tag="PWZ48_002589"
     CDS             complement(1581..2129)
                     /locus_tag="PWZ48_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2262204.1"
                     /translation="MKRIIKIFGVLGVFLLGLSTFVSTGTSVYATEKVNTVDLEIKNK
                     LVDELTFYFDTVGFLDEHGVYHIKDFGAIRERISQDNEQGRAARVFYEHIIVPRMEKV
                     DWKRYAGCVAINSLPGGGVAWAIGTQGDFIEALKAFNFEKAADLLLKIAEKQLGKAAA
                     KELAKVNIVAGIAAAAASCALE"
     gene            complement(2174..2506)
                     /locus_tag="PWZ48_002590"
     CDS             complement(2174..2506)
                     /locus_tag="PWZ48_002590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262205.1"
                     /translation="MSEEKKEIVESLVALKDSLSKIEYVDRQEIQKLIDDTIIEIQDA
                     RCEGIKISVALSKVIEKMNRSLAFNGLKLDRQTSLIWDHLKDLYDKSKRSERTAVSIL
                     KGLWGMNS"
     gene            complement(2520..2777)
                     /locus_tag="PWZ48_002591"
     CDS             complement(2520..2777)
                     /locus_tag="PWZ48_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003653123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262206.1"
                     /translation="MKKLIGYSVMCIGFVGVGLFVSTESASAAEWYNPIWYNPNIECQ
                     KGYPYGPTNLKNGKCRVNWNGVVDSVITGSMDSALHGFTGH"
     gene            complement(2972..3514)
                     /locus_tag="PWZ48_002592"
     CDS             complement(2972..3514)
                     /locus_tag="PWZ48_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262207.1"
                     /translation="MKKGIILVFSWLCLIGLTGCHWFSSEKQKTQLRTTEETIEAVKE
                     TSGTLQSTESNSQIGSLEDETAQQMQIDEMTKNFLKDFARKWSNFTDVYKRNQAVRDY
                     MTDRCIKDNSIDVDPHVQMKTTGKLYQVTQDIENPDQYLLFGEETMNKVTQFVLLQLK
                     VDQEAQKIDAIKIYYVRSAY"
     gene            complement(3535..4626)
                     /locus_tag="PWZ48_002593"
     CDS             complement(3535..4626)
                     /locus_tag="PWZ48_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2262208.1"
                     /translation="MKKGCLGCLLFFILPFFLLPFFFSGELSEHGGEATGLAVAFNGE
                     YTEGLPIFSEIKGRGPITDEHAQAAVGAAVKYQLLPSVILSQLGYESAYGQSQSGRND
                     TNFFGITWFPGCPYPQGSARGIGGSEGGFYMKFPNLKACYDYYGFMVASQSNFNQCVG
                     NKDPGSCLLILGRGGYAAAGITEGSPYYRGAMSIITSYNLTEYDTFAIKHWNSLPTKK
                     TSLIAGKSKGNAKVLDRIIGRSVNNGECYGGTAWYVSQLGGPQLMGSGHMFAMLIGED
                     YNWTTYGWQVIMNPKPSDLQAGDVVNWQAGGALSPGIYGHTGIITDVSYGGQHFSSVE
                     QNAEQGRIMARYQRTYNQTKIRSLVRKVK"
     gene            complement(4634..6844)
                     /locus_tag="PWZ48_002594"
     CDS             complement(4634..6844)
                     /locus_tag="PWZ48_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262209.1"
                     /translation="MAYIKHKEKKWCVLFFLTIGFGLLFVGGLVYAAEKQVDQSIYEI
                     GKFHIDTFSSYLSDSEFSLGKMLFGANGTDFLKMMTNMFFSLSKLIWQGLDFVIEKLY
                     EGAAMNELIHQFFTFSDTIYSKIYDPIGAGIVVLYVLYLFTLRIFRGKHYAKMVFIRF
                     LFVVIFSMVWFGYGSNVPSKGEAFTKGLNDLSVEVEGFIFSATNSLDELATKDVNGVL
                     VPTTQKEAIQKVRQLYYKAAVVDPYILLNYGTTNLEELYQAEIEPQEFLAKEASSEEL
                     SDIETKVTTAAGEDRGKKKAYRVYIQPTKAVYKAVVGSMVPLLNLSIGLPLVVIGMIR
                     FIFQMLVLCLLLVIPFSLLASFVPVFDYMLMNTLKSFLGLMFQKSFYSVIILVAFLIY
                     NIVDDLIPMNTVFGFLGNMFVKALLSILLIVKRKDLLKKLGLGAADQTINNTKNIASH
                     SLSQMKQLPGQLSTAGQNLAIKGAGLAGNVYRATNPLASAAIHGFQKFQQKRTPQESV
                     KGRQVVNPFELGGRSNQALPYSGRHVRYHGVEPEMSSTKSKTEWNSGLSNGLIPNSSM
                     NSVRPKQQVQSLAQLSKQQGNNQRKPLLNKGIAFVGQSIPLPSGKVPVPQLTAAGSLS
                     QQIRQVKNQAKNGSYPAMQQLLNQYSVPNSSVATLSGNRSVQQPGQRSSESKPSISAP
                     KSLQPAFSSLDKPTEQSEKIEKDPKTLPILDAKPGTDFTLPKGRTVQKPLSKKG"
     gene            complement(6860..>7740)
                     /locus_tag="PWZ48_002595"
     CDS             complement(6860..>7740)
                     /locus_tag="PWZ48_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2262210.1"
                     /translation="VLDPIVCLSRVDAKQVAQDMIDELSPLGTNHLLKGAVLKGIQEV
                     LVQKEAGQQVGLMHVVDYLEQMEQKEVREYGEFLRLTVEDSILRLGFSYGENPGLDFN
                     AKTTILEIQDLKLPNDNVRPELYTDADRKSLCLMISLGRFCEMFGKRDSSKKTAIYFT
                     EAWVFNNSNAGRSIIAAMARVGRSQMNQLVLDTQFIGDLGSDKEKGNFGVVFAFDEDS
                     EREKILNHLRLEVNEANLAEMSHMIKGQCWMLDPYGRVGKLNVHSLFEEMTAALQTTE
                     KTSHSQVEEQDTLIMQ"
BASE COUNT         2424 a         1615 c         1062 g         2639 t
ORIGIN      
        1 tcagaaaaat atcgcttgtt ttcttattat gtaatatatt tcataaacta ttctgcataa
       61 caactattgt ttgtactttt tacattggat aactaattat gactgctgat aacttttgat
      121 attaaactag tatctccaat acaaataaaa aatagatatc ttgttcatca aaattagttc
      181 gtggatactt accttcattt acatcattca gcggtttccc attttctttt attctattat
      241 ttcattttct aatagacttg gcttcatttc gcaatacttt ttttcaatag gcatcaaatt
      301 ggtgttgttt agatatttag ttgtagtttt gtgacatgat aagtatgctc ctttttctct
      361 tttccactaa tacaacgatc aaagggtgct tttttcaaaa aactagaaaa atcaaaataa
      421 gattcgtatt ttgattctat atatcctgtt tcaactacat cttatatttc acaatagaat
      481 aaatagcctt ttgcatagct gcttaactta taagaaattg caatttactt cttcaaagtg
      541 aaaacgaata tgagtaaaat aaaattattc ttcttacaga taattaaaaa tagcgcgtaa
      601 caaaaatcgg cattcaatca cataattagg attgatgccg attacttata ttctacgtat
      661 agttttagta ttcttatttt ttatcaccag taaccaattg agaacagccc taaagcagaa
      721 tagtctttta tctcaaaacg cttaatttca ggcttaggaa tatgaataat cttttgagta
      781 tattctttct cttttaatct tactgaagaa ataccactgc ctatcttttt tacaaatatt
      841 tcatttggtg tacagactaa taaaacaata tttgtaatca atccttgcac taaagaatta
      901 aaaggaagac tatttaaaac agggtgtgca taactataac atgtgaagta gttattttga
      961 actttaactt ttaaaaactt taaaaataat cttatttgtt cttctaaaca atcatctata
     1021 ctcatataat cactccacta attaatctcc ccaaaaagac acagcattaa gagatatacc
     1081 aacacaacag tgcaaataac tctacatctt ccttcgcaac cttatttcaa taaaccataa
     1141 aaattatttt ttaatagaaa ctgaaaatta aaactagaaa aagtagtttt accgaatgta
     1201 agagcatcat caccatcaat atagaaatac attcttttcc agttacattt aatttttaaa
     1261 caaaattctc cccaaaaagg aactttttct agtgaaaact tggtaatctc tttgtaatta
     1321 attcttgtta aagaataatt aaacacttca tttttacttg ttattttttc attgactata
     1381 actagttcat ctggtaaaaa aattaaccaa caaacatcga caaacagtaa atgatataat
     1441 ccatttccac ctccaagcat attcaatact ggtgaacatg ccttagaaac acgaatatag
     1501 ttatggttta aatagatatt ttctttaaac attgcttgtt taaaagccat atgctcttgt
     1561 tctaaacttt tcataaactt ttactctaat gcacagctag cagcagctgc tgcaattcct
     1621 gctacaatat ttaccttagc taactcttta gctgcagctt ttcctaattg tttttctgca
     1681 atttttaata ataaatccgc agctttttca aaattaaaag cttttaatgc ttcaataaaa
     1741 tctccctgcg tacctatagc ccaagccact cctcctccag gaagagaatt aatggctaca
     1801 catccagcat atcgtttcca atcaactttc tccattctag gaacaataat atgttcataa
     1861 aagactcttg ctgctcttcc ttgttcgtta tcttgactga ttcgttcacg aatagcacca
     1921 aaatccttta tatggtatac accatgttcg tctaaaaaac caactgtatc aaaataaaaa
     1981 gttaattcat ctactaattt attctttatt tctaaatcaa ctgtattcac tttttctgtg
     2041 gcatacacac ttgttccagt actaacaaaa gtacttaaac ctaataagaa aacacctaaa
     2101 acaccaaata ttttaattat tcttttcata atttctcctc cataaaaaat aagttacttt
     2161 ttaaatatct tgtttatgaa ttcattcccc aaaggccttt taagattgat acagcagtcc
     2221 gttcacttct tttagacttg tcatacaagt cttttaaatg atcccatatc agactagttt
     2281 gtctatctag cttcaatcca ttaaacgcta atgaacggtt cattttttct attactttag
     2341 ataaagctac agaaattttt ataccttcac aacgcgcatc ttggatttca ataatagtat
     2401 catcaattag tttttgaatt tcttgcctat caacatattc aatcttagaa agagaatctt
     2461 ttaaagctac taatgattca acgatttctt ttttctcttc agacattttc ttcctccttc
     2521 tagtgacccg taaagccatg tagagcagag tccattgaac cagtaattac actatctaca
     2581 acgccattcc aatttactct acattttcca tttttcagat ttgtcggtcc atacggatag
     2641 cctttttgac attcaatgtt cggattatac cagataggat tataccattc agccgcagaa
     2701 gcagattcag tcgatacaaa caatcccaca ccaacaaatc caatacacat cacactatag
     2761 ccaattagtt ttttcatctc gtatccatct cctaaaaaat agaatagttc ttatagttat
     2821 tcataagaca tattttttcc gtttttgttt tatcctttct gtcctataac aacttttttc
     2881 aagtaaagaa tgacatagtt tttcttaaaa aaacagtctg tttttttcta tttcttcggt
     2941 gtataataaa tatgagtgtt cattttttat tttaatacgc actccgtaca taataaatct
     3001 tgatcgcatc aatcttttga gcttcttgat ctactttcaa ttgaagtaat acaaattgag
     3061 taaccttatt cattgtttct tctccaaata acaaatattg atccggattc tctatatctt
     3121 gtgtcacctg gtaaagtttc ccagttgttt tcatttgtac atgcgggtct acatcaatac
     3181 tattgtcttt aatacaacga tctgtcatat agtccctcac agcttgattg cgtttataaa
     3241 catctgtgaa attactccat tttctagcaa aatcttttaa gaaatttttt gtcatttcat
     3301 ctatttgcat ttgttgcgct gtctcgtctt ctaatgaacc aatttgactg ttactttcag
     3361 tactttgaag agttccactt gtttctttta ctgcttcgat tgtttcttct gtcgttctca
     3421 attgtgtttt ttgtttttca gaagaaaacc aatgacaacc agtaagacct atcaaacata
     3481 accaactaaa tactaaaatt atcccttttt tcacgtttct agtcactgcc tttcttattt
     3541 caccttacga accagactac gaatttttgt ttgattgtac gtgcgttgat accgagccat
     3601 aatccgtcct tgctcggcat tctgttccac actagagaaa tgttgtccgc catatgaaac
     3661 atctgttata attccggtgt gaccatagat tccaggagac aatgcaccac ctgcttgcca
     3721 attcaccaca tctcctgctt gtaaatcact tggttttggg ttcatgatta cttgccaacc
     3781 ataggttgtc cagttataat cttcccctat taacatcgca aacatgtgtc cagaacccat
     3841 taactgtggg ccacctaatt gagatacata ccatgccgta ccaccataac attccccatt
     3901 atttacggat cgtccaatga tccgatccaa aaccttcgca ttgcctttac tttttcctgc
     3961 aataagcgaa gttttttttg ttggtaggct attccaatgc ttgatcgcaa aagtatcata
     4021 ttccgttaag ttataggacg ttatgatact cattgctcct cgataatacg gactcccttc
     4081 ggtaatacct gctgccgcat agcctccacg acctagaatc aataaacaac ttccaggatc
     4141 tttattgccc acacattgat taaagttgct ttggctcgcc accataaagc cataatagtc
     4201 ataacaagct tttaagttcg gaaacttcat atagaaacca ccttcagaac caccaattcc
     4261 acgtgcagaa ccttgtgggt agggacaacc aggaaaccag gtaatcccaa aaaagttcgt
     4321 atcgtttctt ccagattgtg actgtccata agctgattca tagcctaatt gagaaagaat
     4381 cacactcggt aataactgat actttacagc tgcaccaact gctgcttgcg catgttcatc
     4441 tgtgattgga ccacgtcctt taatctctga aaaaatcggt aatccttcgg tatattcccc
     4501 attaaaagcg acagctagac ctgtagcttc tcctccatgc tcagaaagtt ctcctgaaaa
     4561 aaagaatggc aatagaaaaa agggaagaat aaagaaaaga agacaaccta gacacccttt
     4621 cttcatttct tcctcatcct ttcttcgata atggtttttg gactgttcgt ccttttggta
     4681 aagtaaaatc agttcctggt tttgcgtcca aaatcggcag tgttttagga tccttttcta
     4741 ttttttccga ttgctctgtt ggcttatcta aagaagaaaa tgcaggttgt aacgattttg
     4801 gtgcggaaat cgacggttta ctttcagacg aacgttgccc cggttgttgc acagaacgat
     4861 ttcctgatag agtggctacc gacgaattag gaacagagta ttggttcaat aactgctgca
     4921 ttgctggata actcccattt ttcgcctgat ttttcacttg gcgaatctgt tgtgaaagag
     4981 aaccagcagc tgttaattgt ggcacgggta cttttccaga aggcaacgga atcgattgac
     5041 caacaaaagc gataccttta tttaacaatg gttttctttg attattccct tgttgcttac
     5101 tcaattgtgc caatgattgt acctgttgtt ttggtcttac tgaattcatg gacgaatttg
     5161 gaatcaaacc atttgaaaga ccagaattcc actctgtttt cgatttggta gaagacattt
     5221 caggctctac cccatgataa cgaacatgac gaccactata aggcaaggct tgatttgaac
     5281 gcccacctag ttcaaaggga ttaaccactt gcctcccttt taccgattct tgtggtgtac
     5341 gtttttgttg aaacttttga aatccatgta tcgctgcaga agcgagtgga ttcgttgctc
     5401 gatacacatt ccctgctaat cctgcacctt tgattgccaa attttgtcca gcagttgata
     5461 gttgaccagg cagttgtttc atttgagaca aagaatgact tgctatgttt ttcgtgttgt
     5521 ttatagtttg atctgcagct cctaacccta gtttctttaa taaatctttc cgttttacaa
     5581 tgagtaaaat tgataggagt gctttgacaa acatatttcc taaaaaacca aaaacagtat
     5641 tcattggaat taaatcgtct actatgttat agatcaaaaa tgccaccaaa ataatcacac
     5701 tgtaaaaaga tttttgaaac atcaagccca aaaaggactt taaggtattc atgagcatat
     5761 aatcaaatac tggaacaaaa gaagcgagta atgaaaaagg aatcacaagt aataaacaca
     5821 acaccaacat ttgaaagata aaacgaatca tgccaatcac tacaagtggt aatccaatac
     5881 ttagattaag caatggcacc atagaaccaa cgactgcttt ataaacagct ttagttggtt
     5941 gaatatagac acgataagcc ttctttttcc ctctatcttc tcctgcagca gtggtcactt
     6001 ttgtctcaat atcagataat tcttcacttg acgcttcctt ggctagaaat tcttgtggtt
     6061 caatctctgc ttgatacaat tcttctaaat ttgttgtccc ataatttaac aatatatacg
     6121 gatcaacaac agccgcttta taataaagtt ggcgcacttt ttgaatggct tccttttgtg
     6181 tcgttggcac taaaacacca ttgacatctt ttgtcgccaa ttcatctaga ctatttgtag
     6241 cagaaaaaat aaagccttct acctccacgg acaaatcatt taaccctttg gtaaaagctt
     6301 ctcctttact aggtacgtta ctaccatagc caaaccaaac cattgaaaaa atgacaacga
     6361 agagaaatcg aataaacacc atttttgcgt aatgtttccc tctaaatata cgaagagtaa
     6421 atagatataa cacatacaag acgacaatcc ccgcaccaat aggatcgtat atttttgaat
     6481 aaatagtatc cgaaaaagtg aaaaactggt gaattaactc gttcattgca gctccttcat
     6541 agagcttctc aatgacaaaa tcaaggcctt gccaaatcaa tttacttaaa ctaaagaaca
     6601 tattcgtcat catttttaga aaatctgtgc catttgcccc aaataacatt ttgcctaatg
     6661 aaaattcact atcagataaa taagaagaaa aggtatcaat atgaaatttc ccaatctcat
     6721 agatcgattg atccacctgc ttttctgccg catacaccaa tccaccaaca aaaagcagtc
     6781 cgaatccaat cgttaaaaaa aacaaaacgc accatttctt ttccttatgc tttatataag
     6841 ccattgtttc acctcatttc tattgcatga ttaacgtatc ctgttcttct acttgagagt
     6901 gacttgtttt ttccgttgtt tgtaatgcag cggtcatttc ttcaaacaaa ctgtgtacat
     6961 tgagttttcc tacacgtcca tagggatcta acatccaaca ctgtccttta atcatgtggc
     7021 tcatttcagc taagttcgct tcatttactt ccaaacgtaa atgattcaaa atcttttctc
     7081 gctcactgtc ttcatcaaaa gcaaagacga caccaaaatt ccctttttct ttatctgaac
     7141 ctaagtcacc aataaattgt gtatctaaca ctaattgatt catctgggaa cgccccacac
     7201 gtgccattgc tgcaataata ctacgtcctg cgttcgaatt attaaatacc catgcttctg
     7261 taaaataaat cgctgttttc ttactactat ctcgttttcc aaacatttca caaaaacgac
     7321 ctaaactaat catcaaacac aaagactttc gatccgcatc tgtatatagt tctggtcgga
     7381 cattgtcatt tggtaatttc aaatcttgga tctctaaaat cgttgttttg gcattaaagt
     7441 ctaatcccgg gttttctcca taagaaaatc ctaaacgtaa aatcgaatct tctaccgtca
     7501 agcgtaaaaa ctcaccatat tctcgaacct ctttttgttc catttgttct aaatagtcga
     7561 ccacatgcat caagccaact tgttgtccgg cttctttttg aactaaaact tcttggatcc
     7621 cctttaatac cgcccctttt aaaaggtgat tggtacctaa cggacttaac tcatcaatca
     7681 tatcctgggc tacttgtttc gcatctactc gggataaaca aacaattgga tctaatacgc
//
LOCUS       DANKEF010000093         7673 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000093 DANKEF010000000
VERSION     DANKEF010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7673)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7673)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7673
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.094"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..519
                     /locus_tag="PWZ48_002596"
     CDS             <1..519
                     /locus_tag="PWZ48_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262211.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESSTTVSDANILANIILDFIFEHDIPFKNGYEILPQNEQYFFYKCLTNRVCCSCGKK
                     NADIHHVDSVGMGNNRKKINNSGRRFMALCRECHTKIHVEGFTTFTTKRKLTAVVLKD
                     SDLKRLGLNIIE"
     gene            527..1042
                     /locus_tag="PWZ48_002597"
     CDS             527..1042
                     /locus_tag="PWZ48_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2262212.1"
                     /translation="MDIEGYEGVYQVSSLGRVKSNRPNYVINKGYVNKEGFIMKPSDN
                     GKGYQIIFLSGKGFRDRRYVHRLVAFHFLKEAYFEGAEVNHKNGDKSNNTVENLEWLS
                     SDDNKKHARKNGLTNLKGPSKLTDLQALAIKRLYTNKLMSSGEIMKLFNVSRHTVLNI
                     ASGKTFSFLED"
     gene            1147..1977
                     /locus_tag="PWZ48_002598"
     CDS             1147..1977
                     /locus_tag="PWZ48_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HDL2262213.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1994..2845
                     /locus_tag="PWZ48_002599"
     CDS             1994..2845
                     /locus_tag="PWZ48_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2262214.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDRLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALEKAKSYTNEVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNTELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2842..3201
                     /locus_tag="PWZ48_002600"
     CDS             2842..3201
                     /locus_tag="PWZ48_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HDL2262215.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQA
                     PLILAKKYRNTFKEERF"
     gene            3216..3377
                     /locus_tag="PWZ48_002601"
     CDS             3216..3377
                     /locus_tag="PWZ48_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2262216.1"
                     /translation="MTTEEVIQMRIRNIQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIRRLEE"
     gene            3374..3679
                     /locus_tag="PWZ48_002602"
     CDS             3374..3679
                     /locus_tag="PWZ48_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262217.1"
                     /translation="MSVDKKATMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            3679..3996
                     /locus_tag="PWZ48_002603"
     CDS             3679..3996
                     /locus_tag="PWZ48_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2262218.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVKKYKEILDKYME
                     DSK"
     gene            3993..4388
                     /locus_tag="PWZ48_002604"
     CDS             3993..4388
                     /locus_tag="PWZ48_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262219.1"
                     /translation="MILKYRAWNKKTQSFIDYGDLVLDLRSGKIYAGDIGLVESTIDV
                     TDQIELMQSTGMKDKNGVEIFEGDVVITSKGAIGYVAYLQQEAGFVVVLKKSDYRLGH
                     RNTGESYDVATAHEVIGNIYENSELLEVE"
     gene            4385..4723
                     /locus_tag="PWZ48_002605"
     CDS             4385..4723
                     /locus_tag="PWZ48_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262220.1"
                     /translation="MIILATDYIDSLKDENGNVPALLNCGIKSKNRIILLRISADLAK
                     YLYLSKTAVYYTAATRYQYKGKTISQDYYDRLISPDMHGKSKSAIKLFGAIEIYADDF
                     PNLWLKEEAE"
     gene            4720..5088
                     /locus_tag="PWZ48_002606"
     CDS             4720..5088
                     /locus_tag="PWZ48_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262221.1"
                     /translation="MNEEIKKSIRYIDRALSQLEPLRIESHKGMSVYPNQYDKMNADL
                     RNAKLALINIKNNKGCQYCKHSEPLNDTSNSNFATAIECEENGYYIATEYDDGDYADN
                     GYSAYIQYCPMCGRKLEVAE"
     gene            5085..5390
                     /locus_tag="PWZ48_002607"
     CDS             5085..5390
                     /locus_tag="PWZ48_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262222.1"
                     /translation="MNVQVYLKRNLARFPRSWDTNSHPSEAEEESYLMSANMIEITRD
                     DADEFDKKRSWYSVSNPMYTAVIEDYHISDRFILVDLERPKKRIKQYRRCGFSTKKW"
     gene            5414..5617
                     /locus_tag="PWZ48_002608"
     CDS             5414..5617
                     /locus_tag="PWZ48_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin PIN"
                     /protein_id="HDL2262223.1"
                     /translation="MNNRHRRITKLRKQELNVLKTKFEKEYGVSAEETYKVASQCVAD
                     ASDAIRKFGISILSDDRKWEEKE"
     gene            5614..5910
                     /locus_tag="PWZ48_002609"
     CDS             5614..5910
                     /locus_tag="PWZ48_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262224.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMQTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKENLAVVRLVDIELINDK"
     gene            5986..6402
                     /locus_tag="PWZ48_002610"
     CDS             5986..6402
                     /locus_tag="PWZ48_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2262225.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLIQYR"
     gene            7118..7285
                     /locus_tag="PWZ48_002611"
     CDS             7118..7285
                     /locus_tag="PWZ48_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262226.1"
                     /translation="MYRPQYLEQKYEVITVQNGNSEIVRKYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            7311..7655
                     /locus_tag="PWZ48_002612"
     CDS             7311..7655
                     /locus_tag="PWZ48_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2262227.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         2827 a         1143 c         1737 g         1966 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac ttccgtttcg aaggtgtcac agggcgcaaa
      121 ataagcctca aagatgaatc gagcacaacc gtaagtgatg ccaatatctt agcgaatatc
      181 atcctagatt tcatctttga gcatgatatt ccgtttaaaa atggatatga aattttgccc
      241 caaaacgaac agtatttctt ttacaaatgc ctcacgaaca gggtgtgctg tagttgcgga
      301 aagaaaaatg ctgatataca tcacgtagac tctgtcggca tgggaaataa ccgaaaaaaa
      361 ataaacaatt ctggtaggcg ctttatggct ctgtgcagag aatgccatac gaaaattcat
      421 gtcgaaggtt ttactacatt tacaactaaa agaaagctta cggcagttgt tctcaaagac
      481 agtgatttaa aaagattggg gttgaatatc attgaatgaa ttatggatgg atatcgaagg
      541 atacgaggga gtatatcaag ttagtagctt aggaagagtt aaatctaatc gccctaacta
      601 tgtaattaat aagggatacg ttaacaaaga aggatttatt atgaaaccat ctgacaatgg
      661 gaaagggtat caaattatct tcttaagcgg caaaggtttt agagatagaa ggtatgtcca
      721 tcggctagta gcttttcact ttttaaaaga agcgtatttc gaaggtgctg aagtcaatca
      781 taagaacgga gataaatcta acaatactgt tgaaaaccta gaatggctca gttctgatga
      841 taataaaaag catgctagaa aaaatggatt aactaacttg aaaggaccat caaaactaac
      901 tgatttgcaa gctttagcta tcaagcgttt atatacaaac aaattgatga gctccggaga
      961 aataatgaaa ctttttaatg tcagcaggca taccgtgctg aatatagctt cagggaagac
     1021 atttagtttc ttggaagatt aagagaaaca caaaataggt gtgactaatt tcaaaaataa
     1081 atatcaaatc aaagggatca agttaaacca ggaaacaatt aaaaagttaa ggataggagg
     1141 ataaatttgg ctgagataag ttggatcaaa cttaaaacta ctatgtttga cgatgaaaaa
     1201 atacgattaa tccaagctgt tcctgagtcg gatgccatca tcgttatatg gattcgatta
     1261 ctagttttag caggaaagac taacgacgat ggtctgatat atatccagag gaacatgcct
     1321 tataccgaag aaatgcttgc tacattgttt ggcaaaaacg taaatacggt tcgcttagcg
     1381 ctaactacat tggcaaattt caacatgatt gatctaagca gtgatggact aattgccatc
     1441 agtaattggg aaaaacatca aaatatcgag ggtatggata aagtaaggct aaaaaatgct
     1501 gaaagaaacc gtaaatacag ggaaagaaag agacaggaac gtctcaaatt ggaaaatgac
     1561 gttagcgtga cgtcacgtga cggtacagat aaagatatag aagaagataa agatatagat
     1621 aaagaagaaa agaaaggtaa gtattctgac gaacacttac gccttgctaa aaagttgcaa
     1681 agtaatttaa ctgaagattt tccaaaagaa atgaacaaag tagatatcga aaaatgggca
     1741 gacacaatca ggttgatgga agaaagagat aaagcatcta tagaagcgat tgagtatgtg
     1801 atcaattggc tacctacaaa tgaattttgg tttggaaata ttagaagtgc taagaaattg
     1861 agagaaaaat ttgagaagct caaattcgaa atcaaagcag acaagaagaa tcataaaaag
     1921 caaagtcaaa aactacagta cagcaatcct agtgaatatg acgacttgcc aatttaaaaa
     1981 ggagatgcat cacatggaaa gcctagcaaa tgctatggag aaactaataa gaagagtatt
     2041 agtgcaaagc ggaaaatgtc cagaatgtag cgaacctttg tatagttggc gagctaaaaa
     2101 taaggatggt tcagaacgtt gtaaaccaac atgcatgagt tgtggttata aagcgttacg
     2161 tgtgaaagag gatatacaga ccgaacggat atataacgat aggttaaaag cacgagcgtt
     2221 gagttttttt caaaatggtt cggtattaac agataaaact ttgtttaaat gcaaaatgga
     2281 aaattatcac gtagtggatc aagaaacaaa aatcgcttta gaaaaagcaa aaagctatac
     2341 gaatgaggtt ctgctgaacc atcctgcaca tttcattcta tcagggaaat caggaagcgg
     2401 aaaaagccac ttatcaatgg ccacagcttg ggaaatactt gagcgctcaa attatgacaa
     2461 gaaaatactt tttataagct atcaagaatt attagagcaa ataaagtttt cttataacaa
     2521 tactgaactg agaaaagaaa ttgaaggatc gcttatagcc gatattaaaa caactgattt
     2581 ggtggttttt gacgatattg gagctgaatt aggtagtggg gtatcaaata gtaggcagtt
     2641 tacaaacaac acgttaaaca cgctcttaga agccagacag aacaaggcaa cgatcatcac
     2701 aacaaactta tctggtcctg aactaagaga agcctacggt gaaagaattg tttctaggat
     2761 atttaagaat tcagaaggtt atgcgctgaa attccaacaa acagcagaca agcgcataaa
     2821 accagtgaaa ggtagtatcg catgaataaa taccgtaata aaaaaactgt tcatcgaggt
     2881 atcaagtttg attctatcgc agaagcagag tattacgatc tagccttgtg gcaagctgaa
     2941 gcaaatggct ggaaagtaaa acttcaggaa agatttgagc tgatgccgaa atttgaacta
     3001 gacggaaaga agtatcgcaa gatcgagtat attcccgact tcacatttta taaaaacggc
     3061 aaacttgtca aagtcgtaga tgtcaaagga atgcagacaa aagactttaa gatcaaggca
     3121 aagttgttct gtcatcaata tcaagcgccg ttgatattag ctaaaaaata tcggaatacg
     3181 ttcaaggaag agcgttttta acgaggtggt ccatcatgac aacagaagaa gtgattcaaa
     3241 tgcgtattcg aaacattcag cgtgaaattg acgatctaga acgaacaaag gcagtgatgg
     3301 tcaatgaaac ggcgagaaag gcaatcgatt tgcacataga gaacttaaga agggaaattc
     3361 gtagattgga ggaatgagcg tggataagaa agcgacaatg aaacgaattg ctgaattaac
     3421 caagtcagaa tcttggcagg aagacaaaga aatagttgca gaagtccaaa agctcggtaa
     3481 atcaatgtgg actgaaaagc ctaaacggaa aacgccgaga aaaattgcaa tctggcatgg
     3541 tgatcgaatt ctagtaacag gtacagctga acagttatct gaaattactg gattaagcaa
     3601 aaacattatc tgggatagag ctaggagctt atggattgat tcaaaaggac gacagtttag
     3661 gtatgtggag gagagataat ggatctcatt acacaataca gtgacatcat cctcaagaaa
     3721 atcatgatga agattcagaa agacaaaaaa tcaaaagaac gagcggaatt agttaagttg
     3781 gaaatggctg aaacaggagc aggagtgcga agtagcaggc attggaaagc agcagcaaac
     3841 attgaatttt attacaacga aattcaaaaa gggttcgatc agatgcgtga gctggatcgg
     3901 caaacaaatt ggagcaagaa acttcatcaa gatcgtttca agtttgtaaa gaaatataaa
     3961 gagatattag acaaatacat ggaggacagc aaatgatact gaagtacagg gcatggaata
     4021 agaaaactca gtcatttatt gattatggtg atttggtttt agatttgaga agcggaaaaa
     4081 tttacgctgg ggacattggg ttagtggaaa gcaccattga tgtgactgac caaattgaac
     4141 tcatgcaatc cacaggaatg aaagataaga atggtgtgga gatatttgaa ggggatgtag
     4201 taattacatc aaaaggagcg attggatatg ttgcgtatct tcaacaagaa gccggctttg
     4261 tagttgtact aaagaaaagt gattatagat taggccatag gaatactggc gaaagttatg
     4321 atgtagcaac tgctcatgaa gtcatcggaa atatatacga gaatagcgag ttattggagg
     4381 tagagtgatt atcttagcta ctgactacat cgattcttta aaagatgaaa atgggaatgt
     4441 cccagcattg ctaaattgtg gaattaaaag caagaataga atcattttgt tacggatatc
     4501 ggctgattta gcaaaatatc tttatctgag caagacagca gtatattaca ctgcggctac
     4561 acgttatcag tacaagggaa aaacaatcag tcaggattat tatgatcgtt taatcagtcc
     4621 tgacatgcat ggaaaatcaa aatcagcgat caagttattt ggcgcaatag agatatatgc
     4681 cgatgatttt ccaaatttat ggttgaagga ggaagcggaa tgaacgagga aataaaaaaa
     4741 tctattcgtt acattgatag agcgttatcg caactcgaac ccttgcgaat tgaaagtcat
     4801 aaaggaatga gtgtctaccc aaatcagtat gacaaaatga acgctgattt acgtaatgca
     4861 aagctggcct taattaacat taaaaataac aaaggctgcc agtattgcaa acacagcgaa
     4921 ccgttgaatg ataccagcaa ctctaacttt gcaactgcaa ttgagtgtga ggaaaatgga
     4981 tactacattg caactgagta tgacgatggc gattatgcag ataatggcta ttctgcttat
     5041 atccaatatt gccctatgtg cggtcgaaaa ttggaggtag cggaatgaat gttcaagttt
     5101 atttaaaaag aaatttggcc aggtttcctc gaagctggga tacaaattcc cacccatccg
     5161 aggcagaaga agagagctat ctaatgtctg ctaacatgat agaaattaca agagatgatg
     5221 cggatgagtt tgataaaaaa agatcatggt actcggtttc gaatccaatg tatacggctg
     5281 taatagaaga ttatcatata tccgacagat ttattctggt tgatctagaa agacctaaga
     5341 aacgaattaa acagtataga cgttgcgggt tttcaacaaa gaaatggtga aaacaaaaca
     5401 gaggtgactc agaatgaaca acagacatcg cagaataaca aaactaagaa aacaggaact
     5461 gaatgtacta aagacaaagt ttgaaaaaga atacggagtt tctgcagaag aaacatataa
     5521 agtggcaagt cagtgtgttg ctgatgcaag tgatgctatt cgtaagtttg gaatttcgat
     5581 attaagtgat gatcgtaaat gggaggaaaa agaatgaaac taaaagacgg attttacgct
     5641 agtagccacg gtatcggcgg tttaatgctg gatatgcaga caaagaaccc taaaacacgt
     5701 aagaaaccaa aattcaaagt cggtgacatg gttcgctgcg aagcagaagg gttcatctat
     5761 ccatttcgtg gatatgtaga acatgtctat aatcactcag cgatcattcg cattgaaaac
     5821 acgatggaat gcgacaagtt attagcgaaa agcaaagaga atttagcagt ggtgagattg
     5881 gtggatattg aactaatcaa tgacaaataa aaaagccgga tcgctccgac taacataata
     5941 aaacagacaa gtttattata tcacataaag gagcggtttg acttgatgca attgttacga
     6001 gaggtagatt tcaaacagac aagatgtaat gcgagagatg tgctgaagaa ctttcggcgt
     6061 ttggagcgta tggcaggtcg ctctttgata gatattaagt cgccgattat aacggatatg
     6121 ccgaaggcgc cgaagcacgg caataaggca gaggacgcga tcattcagat gatggatata
     6181 gaagcagaga gagatgcgat tctagcggct ttgatggctc ttagtctgat tagtcgtcag
     6241 atactctact acagcttctg cgacgtaaat aagcattcta attatgaaat aggtcaattg
     6301 atacgaggat acggagagaa gaatgtagag aagttgaaat ccatcgcatt gattgaattt
     6361 gctgaagcat ataaaaaagg cgtattaatt cagtatcgtt gattttgtag ggtttttgta
     6421 gggatagtgt agggtttttg agcggtttaa cgtgatatta tggtagtgtc gaaagattag
     6481 gaaacaggac ttcgacaaaa taaaatgtaa gggaggaaat ctccctcatc gtttaattaa
     6541 gcttcgatag acagcagcgg aaatattaag aataaggatg tgaattttaa ctccttctaa
     6601 attgttctta ttatctatca tccgttgctg tctattgtca ttatgtcact gtggcggaaa
     6661 gggtagacgc ttaaaaataa ggtcaatacg tcgagggata gccttaacgt tttatgattt
     6721 gaccatgcaa tgttcgattc attgccagtg acttaaggaa cctacggaaa caattcatct
     6781 tatcggatgc cgatgaattg gctgactagt cgggatgcta ctagcagtta gaaggcataa
     6841 aatactagcg cagacgtgcg ccactctcag gtgtaggtta ggagagaaac attagttggg
     6901 gttattagga atacgataac ctgcttgcga caaagctttg tactgtcgcg ttggtcatga
     6961 acagagacgg tattctgttt cagtattcgt tagcaaccga gggatgtggc agtggtgagg
     7021 tgcaggaagt atcagacttg tttgtgtgca agttactatt acatattaga tcactctttg
     7081 agtggtcttt ttattttgca caaaggaggt aacaacaatg tatagaccac aatacttaga
     7141 acagaagtat gaagtaatca ctgttcaaaa tggtaacagt gagatagtac gaaagtatag
     7201 aagaccaata aagagcgata catataaacg aaaggaaaac aatgaagtta ttccattgta
     7261 tggcaaaaga atagctaagc attaaataag attgcgaaag gagacgggac atgactgagg
     7321 aattctatag atggctatta cagttgataa gagaagatcg tttggttaag ttctatcagt
     7381 ctcctaaatg gcgcaggctt agagagaaag cgatgaaacg agatcactat gaatgccaag
     7441 agtgtagaag actaggtaag tatcatagag tagagaacgt tcatcatata aaggaagtca
     7501 aggatagacc tgacttagct ttagatttag ataatcttat ttgtttatgt gttgaacatc
     7561 ataatgaagt tcatggcaga tatcttacag cgttagataa acaagagaag aagatagaaa
     7621 gcttcgctaa cttcgatgca agtgaaaggt ggtaagtgca tgatcatcaa tga
//
LOCUS       DANKEF010000094         7600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000094 DANKEF010000000
VERSION     DANKEF010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.095"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(943..1356)
                     /locus_tag="PWZ48_002613"
     CDS             complement(943..1356)
                     /locus_tag="PWZ48_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HDL2262228.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="PWZ48_002614"
     CDS             complement(1812..2297)
                     /locus_tag="PWZ48_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262229.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="PWZ48_002615"
     CDS             complement(2436..2651)
                     /locus_tag="PWZ48_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HDL2262230.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="PWZ48_002616"
     CDS             2875..3675
                     /locus_tag="PWZ48_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDL2262231.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="PWZ48_002617"
     CDS             3694..5562
                     /locus_tag="PWZ48_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2262232.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGHYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="PWZ48_002618"
     CDS             5555..6046
                     /locus_tag="PWZ48_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDL2262233.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="PWZ48_002619"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="PWZ48_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDL2262234.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..>7600
                     /locus_tag="PWZ48_002620"
     CDS             6606..>7600
                     /locus_tag="PWZ48_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDL2262235.1"
                     /translation="MYGGIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1214 c         1444 g         2236 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacatagaag aagggatata taaagtaatg aaacatttca agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggccatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtggtatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgtttccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg
//
LOCUS       DANKEF010000095         7574 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000095 DANKEF010000000
VERSION     DANKEF010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.096"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(69..1172)
                     /locus_tag="PWZ48_002621"
     CDS             complement(69..1172)
                     /locus_tag="PWZ48_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HDL2262236.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            complement(1262..2218)
                     /locus_tag="PWZ48_002622"
     CDS             complement(1262..2218)
                     /locus_tag="PWZ48_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HDL2262237.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            2499..4433
                     /locus_tag="PWZ48_002623"
     CDS             2499..4433
                     /locus_tag="PWZ48_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDL2262238.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            4890..5306
                     /locus_tag="PWZ48_002624"
     CDS             4890..5306
                     /locus_tag="PWZ48_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HDL2262239.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            5320..5886
                     /locus_tag="PWZ48_002625"
     CDS             5320..5886
                     /locus_tag="PWZ48_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HDL2262240.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            5909..6895
                     /locus_tag="PWZ48_002626"
     CDS             5909..6895
                     /locus_tag="PWZ48_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HDL2262241.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(7050..>7574)
                     /gene="scm"
                     /locus_tag="PWZ48_002627"
     CDS             complement(7050..>7574)
                     /gene="scm"
                     /locus_tag="PWZ48_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HDL2262242.1"
                     /translation="TSASSDSTDTTSTTSDSTDTSASSDSTDTSTTTSDSTDTSASSD
                     STDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSD
                     TRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVI
                     GLRITFMSYKKSKR"
BASE COUNT         2147 a         1453 c         1572 g         2402 t
ORIGIN      
        1 acactagcca cctttgttcg tatttcaact acttctaaaa agatttttag tgtcatggtt
       61 cgtactcctt attttagttc agaacggtca cccatcgtgt aatgggagtc ctccgataat
      121 ttgaaggtgt ctttgattgg atcggtcaca tatactttat cgtcttttgt cacgaagatc
      181 gcttccgtcc cgttattcaa ttcgctttcg acgtattcca gtcctttttt cacacccatt
      241 gaaaagactg ctgtagacaa cccgtcacca tcaattgatt tatctgttat gatcgtgaca
      301 cttgcgatat cattgtcgaa cggataaccc gtttttggat caaacaaatg atggtatttt
      361 ttgccatcta cttctaaata acgctcgtaa attccagagg taactaatgt tttgttccgt
      421 tctttaatag aacctaaaac agatccgcga gccatgttcg gatcttggat gccgactgtc
      481 cagtcttggt tttcgcctct tgggctgtgg cctaagacat agacattacc gcccaagtca
      541 acgatggcag tggtcactcc ttgttttttc aagactttca ctacttcgtc cgtaatgtaa
      601 cctttggcga ttgcacctaa atcgatgatc attccttttt cttttagata aacagttttt
      661 tctttgtcat ttagttcgat cttatgataa tccactaatt tcaatgcctg atcgatttct
      721 tcttgggaag gttttctggc gtcatcaaaa ccgattcgcc ataattgtgt gattgcacca
      781 atcgccatat cgaatccgcc ttgagaatct tggctatatt cgtaggcccg cttgactaac
      841 gtgtagacgt catcagaaac ttttactggt ttgatcccag cttgctcatt cacttcatcg
      901 atctcagaac ctttttgatt gatcgtgatt ttatcaccaa gttcttttac tcgatcgaag
      961 gcaggtttca atgctgattc tttgccttca tcatacacac ggatacgaac gtaagtccct
     1021 aacaagaact gttcatcaga atatggatct ttcaatatct tggtagctgc tgaactttca
     1081 gtggttgttt tatttgctga gttgctgcag ccagttagca ccataaaaga gatgcccagt
     1141 gtgattaagg aaaatagtag ttttttccgc atctttttcc tctcttcatt ttgaaaggtg
     1201 agacgactgc ctaatctggc tatcgtccca ccctccaact tttattagta tgattatgtg
     1261 attatttttc tgttacgata ttatctactt cgattgtttg agtgtcgcct ttttcagctg
     1321 cattcactaa ttgttgtgcg tacatgatga atgaatgtga gctgtgtgta gcacctgtaa
     1381 caacttctac attagcaggt gagccatctt cttcgcccat tgctttgacg aattcgtcgt
     1441 tcagagtagg gatgtaagtt tttggttctg taccgctttt tgctttcata ttttcattat
     1501 attctgtatc atcttgtttt gatttaccgt ctttgtcgat ataatcaaag tttgattcag
     1561 ttactttgcc gccagcaact gtcatttcga attgcacacg gtagccgtta gagtagttct
     1621 tttcttcaag cttgtaagtg ccgtctttca aatcagcacc attgtcgatt tcgattgtat
     1681 ctgtgttacc agcttgagca gcttggatca attgttgtgc gtagttttgg aagctttcag
     1741 atgagtgagt agctccagtt acgacttcca caccgcttgc gctttgtgct tttaagaatt
     1801 ggtcattcaa ctcaggaatg tatttttctg ggtttgtgcc agattttgct tccatgtttt
     1861 tgttgtattc tgcatctttt gtttttgatt cgccgttttc attgacgtta tcatattttg
     1921 attcagtgat tttgccgtct tttacaacca tttcaaagac tgcacggtaa ccatttgaat
     1981 aatttttttc ttctaattta tatgtgccgt cttgcaaatc tccgccagct acaacttttg
     2041 cagatgattc tgttgtgctt tttgcagtcg aagattgtgc tgtagatgat tcagaagaag
     2101 agtttgagct tccgttgtca tctgcgccgc aagcgcctaa cactagagct gaaaatgcta
     2161 atacggcaaa tccagttaca aaacgttttt tcttcatttt aataccccac cattccattt
     2221 ctagtccaca atttttcttt gtgattcatt atacattcta atctttttag cctataagtc
     2281 aactattttt gataaataac ccatgtaata tttcaaacgt tttctttcag caaatttcgt
     2341 gaaaaaacgc tttattttaa aatttgaaaa aaatccaaaa ggagttagac aaaagaacag
     2401 aacatctata taatatatgg agattgtgta ataagtatca aactaaaaga attatcatat
     2461 attcattata tagatagtct ataaggagcg gatgatcaat ggcaaagcaa aatattgtcg
     2521 ttgtaggagc aggatatgcc ggtgtttcag ctacaaaata cttggcaaag aaactgaaaa
     2581 aagaagatgt agcaattact ctgatcgatc gtcattcgta ccatactatg atgaccgagt
     2641 tgcatgaagt agctggcgga cgtgtagaac cagaagcaat ccaatacgat ctgcagcatt
     2701 tgtttgctcg tcaaaagaat gtacaacttg tcacagacac tgtaatcggt atcgataaag
     2761 aacgaaaaat cgtcaagacc aaattaggtt cctatccttt tgatcagctg atccttggaa
     2821 tgggcggaga gccgaatgac tttggtacac ctggtgtcaa agcacacgga ttcacccttt
     2881 ggtcatttga agattctgta aggattcggg aacatattga gaagacagta gcaaaagctg
     2941 cattggaacc agatgatgaa ctgcgtaaag ccatgctgac tttcgttgtc tgtggttctg
     3001 gatttaccgg tattgaaatg attggggaac tgatcgactg gaaaaatcgc ctagccaaag
     3061 attataaatt agatccaaac gaaatcacac tgatggtcgt agaggcaatg ccgacgatct
     3121 tgaatatgtt ggatcgaaat gatgctgcaa aagctgaacg ctatttgcgt aagcacaacg
     3181 tggaattatt attgaatgca ccaatcgtag aagtggctgc tgatcatatc aaattgaaag
     3241 acggacgcaa cattccgact cacacactga tttggacagc tggtgtcaaa ggaacttctg
     3301 acgctgctga ctttggatta gaatcagcac gcggacagcg gttgatcgcc aatgaatata
     3361 tgcaggcaaa aggctacgaa gataaaaaca tctatatcat tggtgattta gtcttttatg
     3421 aagaattccc aggaacaccg acaccgcaga tcgttcaagc tgctgaacaa acaggacaca
     3481 cagccgcagc gaatatcgtg gcttcgatca aaggcggaga aaagcataaa tttaaaggaa
     3541 actatcaagg atttatggtt tctattggtg ccaaatgggg ggtagctaac ctatttgata
     3601 agatccattt aagcggattt ttagctatcg taatgaaaca tatcgtgaat ttgaaatact
     3661 tctttgatat tcgttctggt tattatatgt tccaatattt aatgcatgag tttttccata
     3721 tcaaagatga tcgcaacgtg actcgaggac attcttctcg ttacggcaat gtcttatgga
     3781 gtgtgccact tcgtattttc tatggtttag tttggttcgt tgaagcttcc aaaaaaatca
     3841 ttgggaatgg tgaagtgttc aaaccaagta cttggtttgg tgaaggatca tggttcactg
     3901 gaaacagtgt gttcccttgg acatgggaat acaatgtaac agatgttgcc agtggtgctt
     3961 ctgctgctgg tgatgcaacc acagctgcca gcggagcagg ggcaagtgcc ggagaagcag
     4021 cgactcaagc tgcacatttt ggtttgagtt atgcatacgg tgaagaacca atggctgtga
     4081 ttgagaagtc accagactgg ttcacaaaga tcatgcaagt gatcatgcca aacaatgatg
     4141 tagcaatgtt cttccaaaaa ttcatggtat tctttgaaat tgccttggca ttagcattga
     4201 ttgctggatt attcacatgg ctatgcagtg cgacaacgat tgtattggtg atcacgttct
     4261 gcttatcagg catgttttac tgggtaaaca tttggttcat ttttgttgct ttcgcattga
     4321 tgaacggttc cggacgtgcg attgggttgg atcgctgggt aattccatgg ctacaacgta
     4381 cactaggtaa ttggtggtac ggtagaccaa gttcccagta cggcaagaaa taaatagata
     4441 aagaacgtta tttgaatgca agatttcaat agaaaggggc tgagacaaac cgtcacagcc
     4501 tcctttttat gtaaaattaa aggaataaga tgcccgagat aagcctccat gagcaagaca
     4561 caggtgtcac aacctctgtt acaacctctt gttataaacg gtgttccaga agtagcttct
     4621 tcgaaaaata agccgaaatt tcacaaaaac ttgaaaagta attttcgtaa attccttctt
     4681 atttcttgaa gttgcccact tctgtcacaa cctcttgtta taaacggtgt tccacctgct
     4741 gcttctcggt aataagccca aagaattaga aaacatggaa agctgttttc tattcttcgt
     4801 tcttattact caaagcaaga cacaggtgtc acaacctctt gtttttatgc taaaattggg
     4861 tgagttaatc tggaaaaagg agaacaaaaa tgagccttaa agaatttgct gaaaaaagtc
     4921 tgctcaaacc ttgggatgtt tttattattt tgcttttgac tgttctttct tttttaccag
     4981 tcatgatttt tggtcttcag caaaacaccg caacagcaga aaaagaagcg gtactgcgtg
     5041 tggatggcac agaaatcaaa acattccctt tgatggctgg agaagagagc aacacatata
     5101 cttacaaaga tgaacacgga gattataact tgatcgagat agatggcgat cgagtacgga
     5161 taaaagaagc agattgcgat gaccagatct gcgtacgtcg aggctgggca acgaaaaatg
     5221 gcgaaaccat cgtctgtttg ccacataaat tagtgatcga agtccgttca acagatggag
     5281 gtgacgaaga cagtctgatt tattagacct tcacacacca tgagtaaatt acaaaaaatg
     5341 atttatattt cgctacttgt tgcacaagga gtgatcattg gtttattgga aaatatgatc
     5401 ccttatcctt tttcttttgc gcctggtgca aaactaggct tagccaatct aatcacgatc
     5461 gttgccattt ttaccatgaa aaaacaagac agcttcttac tactttggct acgactattc
     5521 ctgactactt tgctaggcgg aacgatttcc acattccttt atagtatgag tggtgctttg
     5581 cttagttatt ttggaatgtt attggtcaag caattaggac caaaacgtgt cagtatcatc
     5641 ggaattagtg cggccggcgg tttcatgcat aatgtcggac agcttttgat ggcttctttc
     5701 attgcacaat catggacagt catgttgtac ttgccagtcc tttcagtttt agggatttta
     5761 tcagggattg caatcgggat cgcagcgaat tatcttttac gacatgtgca gacacttcaa
     5821 cgattccaaa aagattatga gcagggaacc aaacaaaaat ggagcccaat gtttaccaaa
     5881 aaataatcgt cacacaagga gtttttttat gaagctacat ccaatgtggg aaaactatcc
     5941 tgctttaagt aaagaattga cagaaacact aaaacggatg gaagaaactg tccgcctgaa
     6001 aaacaaacct gtcgaacaag ccatcaaaga aacgatccat gccggcggaa agctgttgcg
     6061 tcctgcctat cagctattgt tctcacaatt tggaccagag caagatcgag acaaagccat
     6121 cgctttggca gcttctatcg aaatgctcca tacggccaca ctgatccatg atgatatcgt
     6181 tgatgaagcg gatctgcgac gtggtcagcc taatatccgt agtcagtttg gtaatgtcgt
     6241 ttctgtctat gcaggagact atttatttgt ctgctgtttt aaactattgt ctgattactc
     6301 cacttccttg aaaagtctcc aattgaattc tcgcagtatg gaaaaagtat tgaatggcga
     6361 attagggcag atggatgatc gctataatta tgaactttct gtaaaagaat atttggaaaa
     6421 tatttcaggt aagacagctg aactgttcca attaagttgt tctgttggcg cttttgaaag
     6481 tggaacaagc gagcgcttcg caaaaaaagc aggagatatc ggactttcta tcgggatggc
     6541 ctttcagatt atcgatgata ttttagatta tacaaaagag agccaggaaa tcggcaaacc
     6601 tgtactagaa gacatgcgtc aaggggtcta ttctttaccg ttgatctact ctttgcaaaa
     6661 aaacaaacca aagctactac cttatttaga gaagaaagcg gcaatgacag aagaagatgt
     6721 cgacgccgta cgcaaaatcg ttgaacatac aggaggcgtg gaagaagcta ggaagttagc
     6781 ggcctcttac acagaaaagg ctttgaagga aataaagaaa ttacctgcca cctctcttcg
     6841 aacaaaagag aatctctttt cccttaccca actgattttg gatcgcaaag actgaatagg
     6901 gaaaggttcg atacgtaaat aaatttctca gaaggaaatc tacgtgtttt ttgacaaatt
     6961 gaattgtagt tcttgtattt atgataaaaa gcaaaaataa agagcaacgt ccgttagcga
     7021 cgttgctctt ttttatcttg aatatttttt taccttttcg atttcttata actcataaaa
     7081 gtgattctta aaccgattac aagtaatata acgcctgcga gtgttatcca attattcgat
     7141 tgacttccag ttttaggaag tgctcttttt gatttgtttc cattgtcatt gcttgtttta
     7201 ttagttaagt taaagttcgt cctgttatcc gaacttgttt ttgagaagga gacacttgca
     7261 tgacgtgtat cgcttgtggt agacgacgtg tctgtactat cgcttgaagt tgatgaatct
     7321 gagctatcac ttgtggtaga ggatgtatct gtactgtcac ttgaagctga cgtgtccgtg
     7381 ctatcacttg tagtggtaga tgaatctgag ctatcacttg tggtagaggt agtatctgta
     7441 ctgtcacttg aagctgacgt gtccgtgcta tcacttgttg tggtagatgt atccgtactg
     7501 tcgcttgaag ctgacgtgtc cgtgctatca cttgtggtag aggtagtatc tgtactgtca
     7561 cttgaagctg atgt
//
LOCUS       DANKEF010000096         7564 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000096 DANKEF010000000
VERSION     DANKEF010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7564
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.097"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..414
                     /locus_tag="PWZ48_002628"
     CDS             <1..414
                     /locus_tag="PWZ48_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2262243.1"
                     /translation="FLMTLAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPL
                     GFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLG
                     YTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            462..2591
                     /locus_tag="PWZ48_002629"
     CDS             462..2591
                     /locus_tag="PWZ48_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HDL2262244.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            2566..3255
                     /locus_tag="PWZ48_002630"
     CDS             2566..3255
                     /locus_tag="PWZ48_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262245.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(3663..4349)
                     /locus_tag="PWZ48_002631"
     CDS             complement(3663..4349)
                     /locus_tag="PWZ48_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2262246.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4485..4679)
                     /locus_tag="PWZ48_002632"
     CDS             complement(4485..4679)
                     /locus_tag="PWZ48_002632"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262247.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(4729..5169)
                     /locus_tag="PWZ48_002633"
     CDS             complement(4729..5169)
                     /locus_tag="PWZ48_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDL2262248.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5173..6018)
                     /locus_tag="PWZ48_002634"
     CDS             complement(5173..6018)
                     /locus_tag="PWZ48_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HDL2262249.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6167..>7564
                     /gene="pepV"
                     /locus_tag="PWZ48_002635"
     CDS             6167..>7564
                     /gene="pepV"
                     /locus_tag="PWZ48_002635"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HDL2262250.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADA"
BASE COUNT         2335 a         1430 c         1604 g         2195 t
ORIGIN      
        1 ttcttgatga ccttagcgat gttcaataat tcagctgcgt atgcttctcc ttatttttca
       61 gctatttttg gagattggat accgcaatca ccattagtct tggcaatcgt atttgctata
      121 ttgacaccac taggattttt ccgcggtcca atgaatctag tcggttcagg ctcagctatt
      181 ttagctgtcg tgttagctgt taatccaacg atgtcgccag cctttttgtt cccgttattt
      241 gcaattacaa cgattgctcc tcagcattta gatattacac aatcttgggt tgcttgggga
      301 ttaggttata caaaagtaac ttctcgagaa tatatgaaga aatccatccc aactggttgg
      361 atcatcggtg cgatcttatg cctgatcacg ttcttattgt acgggaacgc ctagtaaaca
      421 aaacgtcgat aaaaacgatt agataaatgg aggaaaagaa aatgaaacgt tttgtgcgaa
      481 tgggaattga tgttggcggt acacatacga aagcagtcgc aatcgataat gcgacgcacg
      541 aaatcatcgg aaaatcttca gtgaaaacaa cccacgatga tgtccgtgga gtagctgcag
      601 gtgtcgttca gtcttttcaa aattgtttgc gggaaaataa tatcagtcca gaagatgttg
      661 tgtttgttgc acattctacc acacaagcga ctaatgcact gattgaagga gacgtagcaa
      721 aagtcggcgt aatcgggatg gcaaaaggtg gattagaagg ttttttagca aaacgacaaa
      781 ctagattgaa cgatatcgat ttagggaata agaaaaaaat agagattgtc aatgcattct
      841 tacctgtcaa aaacttaaat gtagaccgtg tttcagaaac gatttcttct ttagaaagag
      901 aaagagcgga ggtcttggtt tcttctatgg catttggagt agataatggg gaaccagaac
      961 gagttgttta tgaagcagca tctgtgaaag cgatccctac aacgatggct tctgacatca
     1021 caaaattata tggattgaca agaagaactc ggacagcagc aatcaacgca tctattttac
     1081 caaaaatgct agatacggca acctcgacag aagactccgt acgagaagca ggggtcaatg
     1141 tttctttgat gattatgcgc ggagacggcg gagtaatgga aatcaatgaa atgaaaaaac
     1201 gacctgtttt aaccatgctt tccggtcctg ctgcatcagt catgggctct ttgatgtact
     1261 tgagagcgtc taatggtgtt tattttgaag taggcggaac aacaaccaat atcggggtca
     1321 ttaaaaatgg acgtccagca atcgattatt caattgtagg tggccaccca acatatatct
     1381 cttcattaga tgttcgggtt ctaggtgtgg caggcggttc gatggtacgg gctaatcagt
     1441 caggaatcat cgatgtcggt ccacgttctg cacatattgc cggtctagat tatgcagtat
     1501 ttacggagac agaaaaaatc aaaggtccta aggttgaatt tttctctcct aaagaaggag
     1561 accctgctga ttacgtcaag gtagtgatgg aagacggaga agaagtgact attacaaata
     1621 catgtgcagc caatgttctt ggattagtgc aagaagaaca tttttcttat ggaaatgtcc
     1681 catctgcacg aaaagccata caggcattag cagactattg tcatacgacg gttgaagaca
     1741 tcgcagagca gatcatggaa aaatcctatg ctaagattga accggtgatt ttagaattgg
     1801 cagacaagta tcacttggag aaagatcaga tttctcttgt aggtgtaggg ggcggcgctg
     1861 cctcattgat tacttacttt agtaataaaa tgggtgtgaa atattcgatt cctgaaaacg
     1921 ccgaagtgat ttcttctatt ggtgtagcac ttgcaatggt ccgagatgtt gtagaacgta
     1981 tcatcccttc gccaagtaaa gaagatatac gatcgttgaa aaatgaagcg atgaataaag
     2041 cgattgaatc aggcgctaca ccagaatcga tcgaagtcca cgtagaaatc gatccacata
     2101 cttcaaaagt aacggctatc gctactggtt ctacggaagt gaaagcaacc gatctaacca
     2161 aggagatcac aacagaagaa gcactagagc ttgctgcaga ggatatgcga ttaaataaaa
     2221 atgaagtttg cttattggaa aatacacctt tcttttatgt atgcggcgaa cagaatcgtt
     2281 caaaaaatgc tggaagtctt cggatcatcg atcaaaaagg atttatcaag gtccaacgtg
     2341 gacatgcttc atgtatgaaa acaactgcag ccaattatat gacagctgtc gaacaacttt
     2401 gggaagatat ggcagtttat caaactgaat tgattgcccg accagaattt tatctctgct
     2461 taggagcaag agtctctgat tttacagcga cggatctgga acaactacag cttttgatgg
     2521 atctggaagt atctactatg gaaccggagg aagaagtgat cgtcgttgcc ggaaatatca
     2581 aacaaacata agttagaaga gttactaaac gaattacaac agatccccga agaaatttgg
     2641 ggattctacc agtttcggcg agatttattt tggaaaaaga ttcctttgtc caagcagaaa
     2701 attcttatac aacaatcaat cgattgtggt atcgaaacgg catgtagcat caaaaaaaag
     2761 tacccttttg ccgatgtagg agaaatctgt gaacaaatgg ctgttccaat cgtttcctgt
     2821 gaatcagagc aaataaacga acgaatcact tttgcaactt atgcagaaga tgaagggatc
     2881 cgattgatga cggagccgtt agaaaaatta aaatgttcag gactagcttc gatctctaaa
     2941 gaaacagcgc aagctttgat tattggacat gaactatttc accacatcga agcttctgtt
     3001 aagggaattt atacgcaaag cgaaaaaatt gtgctgtggc ggttgccttt ttatacacat
     3061 caatcaaaca ttcgtgcatt gagcgagata gcagcgatga gtttttcaaa agagatgaat
     3121 caaagcgatt tttcgcctta tgtattagag gcagtcttac tttggccata taatgaaatt
     3181 cgtagccaag ggatcttaga agaaataaaa gaaatagaaa aaagatgtac ggagtatgat
     3241 ttagattaca aataaagaga ctgtgacaaa agtcgtgtca cagtcctttt tttccgaata
     3301 aacggtgttc tcctgtcaac tcctcggtca caagtcaaaa tattcaacaa acatgagaag
     3361 ctgttttttg aaattgttcc ttgtgactcg gagatgaacg gcaggctcac gacctcttgg
     3421 aaaaaggtgt tcaaaagctt gcatctgcgg taataagaac agccaagtta aaaacataag
     3481 aagctatttt tacttggctg ttcttattac ttgatgcagg gcagcttttt cacaacctct
     3541 caataaacgg tgtgcaatca gctgaccctc ggtattagct caaaaaaaac ggcaaaatat
     3601 gaggaactat tttgccgttt atttctctaa tactcaggtc aaaacgctga ttccaaaacc
     3661 tcttatttga tgggtctgtc ctgaatatct tcaaaataat cggggtaatt atgaatcacg
     3721 tattcttgct cctcgataat catatgtaaa tgcatcgtgc aaagaaatcc gacttcatca
     3781 aagttatgat caatcattga ttgaagcatt gtctgatgtt catctaatac tcgaccccag
     3841 tctaattctg aaatcgtcaa acgtaaccat ctgaaacggt tcaagtgcgt actataactt
     3901 tctagccaat cccaaatttc tcgttttcca gcaatttcat aacaagttcg gtgaaaatca
     3961 gtatccaaat caaaaaaagc ttttttattt tgagcgatca ctgcctgatc ggtttgttct
     4021 agtatttttt ctagtttagc ttgatcttgc ttggtcattt tcgcactgca ttcttgcagg
     4081 atcggttttt ccaacttttc ccgcgtataa cgggctaatg aagcagatcg gatatcgatt
     4141 tttgaaacat aagtgccgga ctgtggaatg gtttttacaa gctcttgatt gcgcagttgg
     4201 atcagtgctt ctcgaatcgg tgtacgcccg atagctagtt gttcttctaa cactttatcg
     4261 gagattttac ttccgggtgc taactcggaa taaatgattt gtttacgtat agattcataa
     4321 gcttgctgtt gcaaattatt gataaccatt taaccttcct ttctatgcag atataatcta
     4381 ttataacata aatcgcttac aaatcgtgtg ggcataaaaa cacagtctgc aaaaaaagcc
     4441 ctgataggca tgcctattag agcctcgaaa agaaaaatat tgaatcaatc tggtcgtacc
     4501 tcttggatcg gtttgacacg accgccgatt cttttagcaa aagcttcgga ttcttttttg
     4561 tctttgaaaa tgagcagatt gtctaagttt ttttcccgag tgcgacagcc agttttatcg
     4621 atgtaaccgt gcattacttt aacgacatac atggggatca cctgcttttt tcttttcatt
     4681 atacgaacta ctcgacacaa tacaagaaaa atgcttagac ggtctttatt aagaatttac
     4741 atcatccaaa gaagcgtacg cttggaataa ctgttctaac tccggattga ctttgctcag
     4801 cttcactaat cgttgcgaat cagaaagtaa aaaacagtgt ttactactgc cagtagtttt
     4861 taattttttg tcttcagttc ccacaatttc gtagctaaaa tccaaccgtg ttcccgtata
     4921 cttttggata gttgggtgga tgctcacttt ttctccgtaa tggatcattt ctttatagct
     4981 gcaatttact tccagaacgg ggatgatgat cccagattct tcgatttttt tatagggaaa
     5041 ccctagatgt accaataagg ctacccgagc ctcttcaaac cagcggatat aattgctgtg
     5101 atgaataatc cccatttgat ctgtttcata ataaaaaggt tctcgcacat aacctgtaaa
     5161 tttctccatg actcatgcct cgttttccat tgatttttta caaaaataat tcatccactt
     5221 cggaagtgct tcgctgattt gtgtcggcaa aacaatatat tgttcttttg ccagctggtc
     5281 tccaatgaaa ctatgaagat aaacagctgc tgcaatcgtt tggtcatttt gaggaaactg
     5341 ggctaaaaat cctgtgatca tcccagctaa agtgtcaccc atgccgcctg ttgccatagc
     5401 agcagaacct aaaggattgg tgtagaagtg ctggtgacca taaatttttg tctcatgact
     5461 tttcaaaacg agcacagcac cgatttcctg ttgcttcttt ttgttttctt tatcccgttg
     5521 ttcttgtatc ggtagagaac tcaaccgttg ccactccatt tgatgaggtg taagtacagt
     5581 ttgttcggga tagggtagca aaagatgatt agcaacaagt aaacttaaag cagagccatc
     5641 aatcaccagc cattgttctt ttgattggtt ttctagtact tgtttgaaac gttccaaact
     5701 cttagaagaa gtacccaatc cgggaccaat caagagtaca tctgcttgct ttattacgga
     5761 aagaaactgg tcagtatccg tccaatctac aatcattgct tctggcagat gactgtgaag
     5821 agcactccga tttttttcat cagttactac agtcgtgaga cccgctccac tgtgtacggc
     5881 agctaaagca ctcataatga ttgctccgcc atattgttca ttgccgccaa tcaaaacgat
     5941 acgtccaaat gttcctttat gtgaaatctt tggacgaggt gtgatgactc ttttcactag
     6001 ttgttcgttt atcagcatcg aaaaacctcc ttttactcat tatagcgtat atttttttta
     6061 gagaaaaaca gataagaaaa ggaattgtca gattttttga actatttttt tctgtaattt
     6121 gtggtatcat gaacacgaag acttaaataa gtggaggatt aactttatga caattgattg
     6181 gcaaaaagaa gtagaagcac gtaaagaaga cttgcttgaa gacttgaaaa atcttttacg
     6241 tgtcaacagc gagcgcgatg attcaaaagt aacaccagat gcaccatttg gacctggacc
     6301 tcgcgacgca ttgaaacata tgttagctta tggcgaacgt gatggattta aagtaaaaaa
     6361 tgtcgataac tatgcagggc atattgatct tggcgaagga gacgaaacgc tagggatatt
     6421 tggacacatg gatgtcgttc ctgcaggaga tggctgggac acagatcctt atgaaccagt
     6481 aatcaaggat ggaaagatct ttgcacgcgg atcaagcgat gacaaaggtc caagtatggc
     6541 agcttactat gcgatgaaga tcattaaaga acttgacttg aaactatcaa aaaaagttcg
     6601 cttcgtagtc ggcagcgatg aagaaagcgg ctggggcgat atggcttatt atttcgaaca
     6661 tgaagaagaa ccagattttg gtttctcacc agatgctgaa ttcccaatca tcaatggtga
     6721 aaaaggaaat gtttctttag ctttacgctt caaaggcgac aatgcaggag attatgtctt
     6781 gaaatctttt gtttccggac ttcgcgaaaa tatggtgcca ggaacagcaa ctgcagcact
     6841 tcaagtacca agtgcagatg cagctatcgc gatggaagaa gcattttacc aattcatcga
     6901 agcaaatcca gtaagcggaa cgattgaagc ggacaatacg tatgtaaaaa tcgaattagt
     6961 tggtaaaggc gcacatggtg caagcccaca atctggtatc aatgcaggtt ctttcttggc
     7021 attgttctta gataactatg aattcctagg atctgctaaa caatttatcc atgtagcagc
     7081 tgcttatgtc catgaagatt tttatggaga aaaactagga gtcgcttatg aagatgaaaa
     7141 aatgggcaaa ttgacaatga atgctggact atttgctttt gaagaaaatg gtacagagga
     7201 agcgaacttc atcaatatga atttccgttt tccaaaaggt gtaacagtag acggattgca
     7261 aagtgacatc gaacaaacag tggggcaaga aggcgcaaca gttactcgag gtgctcgcgt
     7321 aatggaacca cattacgtac caatggatga tccattggtg gctacattat tgcaagtcta
     7381 tgaagaccac actggagaaa aaggctacga acaaatcatc ggtggcggaa cttacggacg
     7441 tttgttgaaa cgtggcgttg cttatggagc gatgttccca ggatatactg atacaatgca
     7501 tcaagcaaac gaatttatga gtcttgatga cttgttcaga gcaacagcaa tctatgcaga
     7561 tgcc
//
LOCUS       DANKEF010000097         7332 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000097 DANKEF010000000
VERSION     DANKEF010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.098"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1037
                     /locus_tag="PWZ48_002636"
     CDS             <1..1037
                     /locus_tag="PWZ48_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HDL2262251.1"
                     /translation="QIGAWMDKDYNITKGENVLIHKPADVTDHFRDPQMFNYEGQLYA
                     IVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGSEYMIECPNLVFVDKKPVLI
                     YCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASEIQNLDYGFEAYATQGFNTP
                     DGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDGKLYQYPVEAITSLRASSEQ
                     FTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLIVDTKEGKIILDRSKAGIQY
                     ATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVFTSRVFPENHQNGIQIKSGE
                     PTGKYFELKY"
     gene            1039..2001
                     /locus_tag="PWZ48_002637"
     CDS             1039..2001
                     /locus_tag="PWZ48_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2262252.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(2171..3597)
                     /locus_tag="PWZ48_002638"
                     /pseudo
     CDS             complement(2171..3597)
                     /locus_tag="PWZ48_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            3840..4295
                     /gene="tnpA"
                     /locus_tag="PWZ48_002639"
     CDS             3840..4295
                     /gene="tnpA"
                     /locus_tag="PWZ48_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HDL2262253.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYRRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            4560..4658
                     /locus_tag="PWZ48_002640"
                     /pseudo
     CDS             4560..4658
                     /locus_tag="PWZ48_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(4881..5111)
                     /locus_tag="PWZ48_002641"
     CDS             complement(4881..5111)
                     /locus_tag="PWZ48_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HDL2262254.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(5341..6159)
                     /locus_tag="PWZ48_002642"
     CDS             complement(5341..6159)
                     /locus_tag="PWZ48_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDL2262255.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            6321..7010
                     /locus_tag="PWZ48_002643"
     CDS             6321..7010
                     /locus_tag="PWZ48_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDL2262256.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            7024..>7332
                     /locus_tag="PWZ48_002644"
     CDS             7024..>7332
                     /locus_tag="PWZ48_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2262257.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     S"
BASE COUNT         2451 a         1194 c         1296 g         2391 t
ORIGIN      
        1 ttcaaatcgg agcatggatg gataaagact acaacattac gaaaggtgag aatgttctta
       61 ttcataaacc tgctgatgta actgatcatt ttcgtgatcc acaaatgttt aattatgaag
      121 gacaattgta tgcgattgtt ggagcacaaa atttagaaaa atcaggcttt atcaaacttt
      181 acaaagcgga tgacaataac gtggaaacat gggaggaagt tggcaacctt gattttggcg
      241 gcactggttc agaatacatg attgagtgtc caaatctagt atttgtagac aaaaaacctg
      301 ttctcattta ttgtcctcaa gggttggata aatctgagct aaattatggg aatatctatc
      361 cgaatacttt taagatctgt caatcgcttg acatagaaaa agtaaaactt gtgggcgctt
      421 cagaaattca aaatcttgat tatggatttg aagcgtatgc gactcaagga ttcaatactc
      481 ctgatggtcg tgctctgatt gtcagttgga ttggactacc tgatatcgat tatccaactg
      541 ataagtatga ttatcaggga gcaatgagtc ttgtaaaaga actttcaata aaagatggta
      601 aactttacca atatccagta gaagctatta catcattacg tgctagttct gaacaattta
      661 cagcaaaagc agaaacaaat aatacttatg agcttgaact aacttttcct ccaaatcaga
      721 aatcggagtt acttcttttc tctgatagta aaggaaatgg acttagcttg attgtagata
      781 ccaaagaagg aaaaattatt ttagatcgca gcaaagcagg cattcaatat gccactgact
      841 ttggtacgat tcgtgaatgc tcaattgata ctaaagaaac aactgctaac atttttgtag
      901 ataactcaat cattgagatt tttattaata aaggagaaaa agtgtttact agtcgtgtat
      961 tccctgaaaa tcaccaaaat ggtattcaaa tcaaatcagg cgaacctact ggaaaatact
     1021 ttgaattaaa atattaaaat ggtagcaaaa ttaactgatg tcgctgaatt agctggagtt
     1081 agcccaacaa ccgtttcacg cgtcattaat aataagggat atttatcaga aaaaacaaaa
     1141 caaaaagttc atgaagctat gaaaactctt ggttataaac caaataattt agcacgtgga
     1201 cttcaaggaa agtcacctca gcttattggt cttatttttc ctaatattag taatatcttt
     1261 tactctgaac tcattgagta tctcgaaata gagttattta aacatggata taaaacgatt
     1321 atttgcaata gtcaaaatga tcctgctaaa gagagagaat acattgaaat gcttgaagcc
     1381 aaccaagttg atggcataat ctcatcaagc cataatcttg gaattgctga ttatgagcgt
     1441 gtgagagcac ctattgttgc ttttgataga aatttagctc ctaatgtttc cattgtttct
     1501 tcagataact ttgaaggggg aaaacttgca gctaagactt tacaaaaaaa tggttgtcaa
     1561 aataccatta tgattacagg aaatgataac acggattctc ccacagaatt acgtgcacta
     1621 ggtttttctt ttcaaaatcc aaatggaaaa attttcaaaa ttcctaataa tctgtctact
     1681 atccgtcgtg aaatggaaat aaagtcaact attataaatc acaaaccaga tggtattttt
     1741 gtttctgatg atttaacagc cattctcact atgaaaattg ctcatcaact taaacttaat
     1801 atcccagaag atttaaaaat tattggatac gatggtacct cttttattga gaaatttttc
     1861 ccacaattga caacaataag acaaccaatt gatgaaattg caagtcttat tgttgatatt
     1921 cttctgaaaa aaattaaagg cgaaaagaca aataaggatt atattttacc aatctcaata
     1981 ctttctggag gaagtattta acgttaaatt aattttttaa tgtctataaa attcgttaga
     2041 aaatacaatt tgtagatatc caattgattt gtaaacaaaa aggagttgtc aacttgtagt
     2101 gacccccaaa agttagactt ttttggagtg gagaaatcca ctcctttttt catgctctaa
     2161 aatgatattt ttacacaact attacggttg ttttacgaaa ttggacggga ctttgccagt
     2221 ttaatttctg ttttatccgt tcattattgt agtaatagat atagctatcg atggctgatt
     2281 ttaattcctc gaaacagtta taaactttcc cgtgaaaaat ctcttgtttt aataagccaa
     2341 aaaaattctc cataagtgaa ttatctaagc agttcccttt ccgagacagg ctttgaaaga
     2401 ttttgtactc cttcaatttg tttctataag ctttcatttg atacgcccaa ccttgatctg
     2461 agtgaaacgt acgacgataa ggacaatcgt tagtggtctg gatagcttac tccaaacttt
     2521 ccattaccgc taatgcattg ggcttttcag agatacgata ggataaaatc tcactattga
     2581 acatatccat aaaaggatct agatacaatt tcttttgacg gataatgcct ttggcatcca
     2641 cttcatggta tttaaattca gttgtatccg tcgtgatttt ctgatggcag atactggtgt
     2701 agaaacgacg atggatccga tttttggcta ctgctccgac ttgtcctcga taagaattgt
     2761 agcgccgtga tttacgtgtg tatgtcgtta cttttattcc tagcttcttg atcaggcgct
     2821 gaactttctt tttattgata gagaaaccgc ggtttcttag ttcattggtc atccgtaaac
     2881 agccataatc cttgtgcttc tcccgaattt taagcatttc cgcttcaatc tcttgatctg
     2941 gatttttacg gtccaatcgt ttttgccagt acataaaggt tgccttaggg aactttaaag
     3001 tttctagaag ctcaactaat ttaaacggtc ctcggaggct tgcgatgatt cgtgcgattg
     3061 cttcattcgt cgttgttggg cttcctgttt tcgcagcttc ctcaattctt ttaaagaggc
     3121 attttcaatt tgaagggaac ggactcgttt ctccaactcc tcgatgcgtt cacgttcttt
     3181 agacgttgct ttagttgtag attttttctt atttttgggt tcatatttct tagacaagat
     3241 aggtggacat cctttcgttt ttgagagtcc atcgatccca ccctcacgga attgatgcat
     3301 ccaactagcg atcaaattag ggttgttcat gttaagtgtg ttggcaactt cacgatagga
     3361 cagctcactt gttagatata actctatcgc atccagctta aattgaacag aatatttttt
     3421 cttttgtcgc tttcttacta gaccctcttc accaaattct tgataggcat tgatccagcg
     3481 cttcacttgt gatggaacct taatggaata cttctttgcc agataatcac ttccgccttt
     3541 accatccaaa taatcatgaa cgatctttaa tttaagttca aaagtatact tagctataaa
     3601 aaataccgac ctccaaaagt tagatttttt ggtctaactt ttgggggtcg gtacaaaact
     3661 taaaagtttt agaccctttt attttcttaa tgacttcagt cagttttgtg cgtaaagcgt
     3721 gcgaaacaag aaaaccgcgc gtttctacgc gtggaagaag tatctttagc gataaaaact
     3781 cctttcgata tactagtgag tggctaccaa ccgctcatca gatcgaaagg agttttttca
     3841 tgtcaaatga cgataaaagt ttagcatata ctcgttggaa ttgtaagtat catattattt
     3901 ttacaccaaa atatcgacga aaagtaatct atgggcaatt acgaaaagat cttggaaaaa
     3961 tacttaggaa gttgtgcgaa atgaaagatg tggaaatcat agaagcacat gcaatgctag
     4021 atcatattca catgcttgtt cgagtaccac caaaaatatg tatatccagt tttatggggt
     4081 atctgaaagg tcgtagtgcg gtactaatcc acgagcagca cgcaaatctc aaatataaat
     4141 acggaaacaa atccttttgg tcgaagagat actatgtgag tacagtcgga ttgaatcaaa
     4201 agacaaatga aaaatacatc cgtgaacaag aagctgatga tcgcattcga tatattataa
     4261 gtaaaagaga gtacgtagaa ccatttaaaa aataaagaga agtaagtgag tgaagatgag
     4321 cagttggcgg tcttttaaaa atcccctttt aaggggcagc gagtgtcgac cccttctagg
     4381 gggattaaaa atacacccgt cctcacgggt gtatgggtta ctgatagact tgattttaac
     4441 tggtgtcaga aacttatgtc cagaaagggt gatgaaattt tggcaattta cggctattca
     4501 agaattagca caagcaacca agattacaaa acacaaattc aaacattaga aaatgctggc
     4561 gcagaaataa tcttcctaga aaatcactgg attatcgaac acctttggaa gtatttttga
     4621 gttacgtaag tattgatgat ctgtctaact taatttgaca attaagagta tatttacttc
     4681 agatggtaag gcaacaaaat aaaatacaat aggcagatga actaataaat aaaatctgta
     4741 ttaaatacag ataaattaat ataaaaatca aaaggatcat aaacttttaa caagtgatga
     4801 aatgggtgga aattgattct tatagacagt ttgtctcata acattgaagt attttgcaat
     4861 tttagcattg aaacactttt ttaatcttca acgttgttct tataaacaag atgattggga
     4921 atcttttgaa ttaacactgt atagcttaga acacccttta tagaaaacat tcgcttagca
     4981 atcttaataa ttccttgtgc ccgatgtcgt ttagattcag taggttcctg ctcatcgatc
     5041 atggaaaaaa tttgaaggat cagatcctta ataaatttat ttaagagagg atgatccatt
     5101 gcttcatcca tactcgataa actagtgata atgagctaca cgtttttctt tcaagttgat
     5161 caatagcttc cacgataaaa atatctttag ataaaatcaa gaaccgtagg aactacgggg
     5221 atatttggtc aataagagcc accgctatta gtaaaaaata agctatcaag tatgctctca
     5281 tcccaagaat tgcctacttc aatcgttaaa aaccgttagg tttagctaag tgggggtagt
     5341 tcactcaata cttttatcaa taatagagcg caaaactttt tctcttaatt caactgaatc
     5401 aatattattg ttttgttcat agccatgaac taatagatca caaatataaa gttgtgatat
     5461 ttttccagct aaagatccac cattaagaaa ttcttcaatg gctgtttgta aaactaagtc
     5521 tgcagacttt cctataggtg aatgaatata gttagttata gcaataattt ttgcgccatt
     5581 ttttttagct attttcaatg aatcataagt atctttcgtt ttcccagata aagaaaaaat
     5641 gattactaaa tcattaactg ttaataaaga agcgacttgg gcttgaaaat gagggtctag
     5701 tacagctttt gcttgaactc ctacgcgaag aaacatattt tctaaatcaa gactagtatt
     5761 tccactagat cccactccaa atatatatat gttttttgat tgagtgatca ttttaatggc
     5821 ttcattcaat ttttcttcgt ctaaaagcct aatagtggaa tagagagctt ctgttaaact
     5881 attagcaatt tcatcatagt actttacact agtcggatgt tctttttttt gactaaagtc
     5941 ttcctttgca atttcaattt ttaaatcaga aaaaccagaa aaaccaattt tttgacaaaa
     6001 gcgaataata gtagcatcgc caacttttgt gtttttctta atatcactca tcgtactata
     6061 aataatagtt ttcccagaat ttaaaataaa atcagcgact tttttttcag acttcgttaa
     6121 aaatggataa ttttctttaa ttaaatagat ataactcata caaactcctc tacaatttca
     6181 acttttctcc attatactcc aaaataataa aaaaaactcc taaatactcc atttttatct
     6241 ttacttttat gaaagcggat gctatattat ttttggagtt attatccaaa aataataaaa
     6301 aaattggagt gtttaggata atggataaaa agaggttttt agatcttatt tcaaaaggtt
     6361 tgattgtttc atgtcaagca ttacctggtg aaccatttta caaagaggaa gaaagtttaa
     6421 tgccttattt tgctttggct gctcaaaggg ctggagcagt tggtatacgt gcaaattctg
     6481 ttcgagatat tattgaaatt agaaagtgtg tatcattacc aatgattgga attattaaaa
     6541 gagattatcc accagaatct ccattcatta ctgccactat gagagaagta gatgaattag
     6601 tagaaaccgg tgttgaggta atcgctcttg attgcactct tagaaatcgg catgatggac
     6661 tatctataaa ttcttttatt actaaaataa aagaaaaata tcctcatcag ttatttatgg
     6721 cagatatttc tacatttgaa gaaggcaaaa atgcatataa agcaggaata gattttgtag
     6781 gaactacttt aagtggatat actgaggaaa gtaaaaaaca agatgggcca gatatagatt
     6841 tgattaaaaa attggtagaa gaagggattt gtgtaattgc tgagggaagg attcatactc
     6901 cagaacaagc aaaagagata aaaaaaatag gagttgcagg tattgttgtg ggtggtgcta
     6961 ttactcgacc acaggaaatt gcatcaagat ttgttaaagc attaagttag ggggagaaat
     7021 aaaatgttta aaaaattttc acaattagga cgcgctttca tgttaccaat tgctatatta
     7081 ccagtagccg gattactatt gggactggga ggagcgttaa caaatgagag tgctataaat
     7141 gcttatccat ttttagatca accgtggttg catacaattt taagtattat gagttatgct
     7201 ggaaatgcag tttttgcaaa cctagcacta atttttgcaa ttggtattgc tgtaggactt
     7261 gcaaatggag ataaaggaac agctggttta gctggtggag tagcttatct agtatatact
     7321 gcaactatta gt
//
LOCUS       DANKEF010000098         6928 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000098 DANKEF010000000
VERSION     DANKEF010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6928
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.099"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..513
                     /locus_tag="PWZ48_002645"
     CDS             <1..513
                     /locus_tag="PWZ48_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262258.1"
                     /translation="VQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            516..1265
                     /locus_tag="PWZ48_002646"
     CDS             516..1265
                     /locus_tag="PWZ48_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262259.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1277..1546
                     /locus_tag="PWZ48_002647"
     CDS             1277..1546
                     /locus_tag="PWZ48_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262260.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1551..1715
                     /locus_tag="PWZ48_002648"
     CDS             1551..1715
                     /locus_tag="PWZ48_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262261.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1708..2010
                     /locus_tag="PWZ48_002649"
     CDS             1708..2010
                     /locus_tag="PWZ48_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262262.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            2007..2168
                     /locus_tag="PWZ48_002650"
     CDS             2007..2168
                     /locus_tag="PWZ48_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2262263.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2165..2470
                     /locus_tag="PWZ48_002651"
     CDS             2165..2470
                     /locus_tag="PWZ48_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262264.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2470..2826
                     /locus_tag="PWZ48_002652"
     CDS             2470..2826
                     /locus_tag="PWZ48_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HDL2262265.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2813..3031
                     /locus_tag="PWZ48_002653"
     CDS             2813..3031
                     /locus_tag="PWZ48_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262266.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            3028..3447
                     /locus_tag="PWZ48_002654"
     CDS             3028..3447
                     /locus_tag="PWZ48_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HDL2262267.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3444..4001
                     /locus_tag="PWZ48_002655"
     CDS             3444..4001
                     /locus_tag="PWZ48_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HDL2262268.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3998..4294
                     /locus_tag="PWZ48_002656"
     CDS             3998..4294
                     /locus_tag="PWZ48_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262269.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4371..4784
                     /locus_tag="PWZ48_002657"
     CDS             4371..4784
                     /locus_tag="PWZ48_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HDL2262270.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIETERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGKIIAYK"
     gene            5093..5245
                     /locus_tag="PWZ48_002658"
     CDS             5093..5245
                     /locus_tag="PWZ48_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262271.1"
                     /translation="MKLSVEGTPEEIQELLQAIGSSKEQKVSVSNSDIDGICNCLNRE
                     KISNPY"
     gene            complement(5242..5517)
                     /locus_tag="PWZ48_002659"
     CDS             complement(5242..5517)
                     /locus_tag="PWZ48_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262272.1"
                     /translation="MYKPYMISYDLNNPGQKYDKVFEIIKEFGAYIKLQKSFWLVKTN
                     LNPNQMCEKLNTVLDNNDSLFICELQKNYQGRATEENWKFINEHIFS"
     gene            5971..6177
                     /locus_tag="PWZ48_002660"
     CDS             5971..6177
                     /locus_tag="PWZ48_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262273.1"
                     /translation="MLGQCFGNHARFDSLPATLNAYDGYDYRKKIVKKLYGATYGNVV
                     SVLSVHHQASVTVAEVEDAAVNGE"
     gene            6373..6540
                     /locus_tag="PWZ48_002661"
     CDS             6373..6540
                     /locus_tag="PWZ48_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDL2262274.1"
                     /translation="MYRPQYLEQKYEVITVHKGNGEKVYEYRRPIKSDTYKRKENNEV
                     IPLYGKRIAKH"
     gene            6566..6910
                     /locus_tag="PWZ48_002662"
     CDS             6566..6910
                     /locus_tag="PWZ48_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2262275.1"
                     /translation="MTEEFYRWLLQLTREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
BASE COUNT         2512 a         1081 c         1522 g         1813 t
ORIGIN      
        1 gtacagcaac gaaacttcat ctatgcgctg ataggcgata tttttatcga tacaggcatg
       61 ccaacggact tctggaagga attcttctac tttcgttttg aaggtgtcac agggcgcaaa
      121 ataagcctga aagacgaatc gaatacaact gtgagtgatg ccaatgtctt agcaaatatc
      181 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacct
      241 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
      301 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
      361 gttgatcatg cagagtacac atttgcagca ctctgcagaa tccatcacac agagaagcac
      421 aaaataggtg tgattaattt caaaaataaa tatcaaatca aagggatcaa attaaaccag
      481 aaaacaatca aaaagttaaa tattggaggg taaaaatgac agaacatcga agttattatg
      541 cgattatacc agccaacgta aggtacgaca aaagacttaa accaaatact aagttgttat
      601 acggagagat aacggccttg tgtaatgaaa gaggcttttg ttgggcaggc aatgagtact
      661 ttgcagattt atatggtgtg aataaagaga ccatatcgcg atgggtaagt gatttgatta
      721 agtttggata cttgaatcgg gaaatcattt acaaagaggg taccaatcaa ataatcaata
      781 ggtacctacg aattaatcaa taccctattg acgaaaaacg caatacccct attgacgaaa
      841 aagtcaaaga taataataca tctattaata atacatttaa taatacaaaa gaatatataa
      901 gagagttacc gccttcgaaa aaatcgaagg ctaagcccgt ccgtcataaa tacggagagt
      961 ataaaaatgt tcttttgtca gatgagcaga tggagaaact caaaacagaa ttccctaatg
     1021 attaccaaga gcgaatcgaa cgactgtcag agtattgtga atcatctggt aagacttata
     1081 aaaactattt ggcaactatt cgaagttggg caaggaaaga aaaaagtgaa cctaagaacg
     1141 caagcagtgg atacaagcgc acaggaagac gagagaagct tcctgaatgg gcaatcgacc
     1201 aagaagccta tctcaagaaa aaagcgctag aacgagctaa tagacaatca aaagcaccat
     1261 tctaagaggt ggaaaaatga agatcgatta tctagaacta attaatgaaa tagcgaatta
     1321 taaaaagggc gaggaattag acgtcctgag agacgtatat gatcaactcg aagaagctgg
     1381 aattgaagga attaagaatg atcgttcgag ttggagtaag ctcagatact atttcgcact
     1441 ttatatcgat acaacacaat taagaaattt agcatatacc aaattactat ttgttgattg
     1501 tgttaaaggc ttgcaaaaac atcttaatga acttgagcag gtgtaatcag atggacctaa
     1561 agacatttac agcacagatt gaactaatgc atcaagaagc tttaagacaa agtgtatcgt
     1621 acgaagacaa gtggctcaac acgttccatg gcggacgtga gagcgcactt gatcaagtac
     1681 tcaaattact gaaaggagaa tgtcgggatg gataagaaag cggcaatgca gcgaattatc
     1741 gaattgactt attcagaaga ttggcaaaat gacaaagaag ctgcttcaga agtgatgagg
     1801 cttggaagag cgatgtgggc agacaagagc aacaagccaa gaccacgaaa aatcgcaatt
     1861 tggcacggtg acaaacttct agtgataggg acagctgaac agttagcaag tctcacaggc
     1921 ttgcacgaga aaatcgtgag gaaaagagca aggtgtggct acacagacgc taagaagaga
     1981 acgtttagat acgtggagga atcatcatga caacagaaga agtgattcaa atgcgtattc
     2041 gaagccttca gcgtgagatt gacgatctgg aacgaacaaa ggcagtgatg gtcaatgaaa
     2101 cggcgagaaa ggcaatcgat ttgcacatag agaatttaag aagggaaatc catcgattgg
     2161 aggaatgagc gtggataaga aagcagcaat gaaaagaatt gctgaattaa ccaagtcaga
     2221 atcttggcaa gaagacaaag aaatagttgc agaagtccaa aagctcggta aatcaatgtg
     2281 gactgaaaag cccaaacgga gaacgccgag aaaaattgca atctggcatg atgaccgaat
     2341 tttagtaaca ggtactgctg aacagttatc tgaaattact ggattaagca aaaacattat
     2401 ctgggataga gctaggagct tatggattga ttcaaaagga cgacagttta ggtatgtgga
     2461 ggagagataa tggatctcat tacacaatac agtgacatca tcctcaagaa aatcatgatg
     2521 aagattcaga aagataaaaa atcaaaagaa cgagctgaat tagttaagtt agaaatggct
     2581 gaaacaggag caggagtgcg aagtagcagg cattggaaag cagcagcaaa cattgaattt
     2641 tattacaacg aaattcaaaa agggttcgat cagatgcgtg agctggatcg gcaaacaaat
     2701 tggagcaaga aacttcatca agatcgtttc aaatttgtag agaagtataa agaaatatta
     2761 gaagagtatt tgaggaggac agcaaatgat aaaaaaactc gttcaattca gcatggattt
     2821 atatgatatc gaatcaggag caacactatc tgtggaatcg gaccatctaa tcataaattt
     2881 tggtggaaag cgccagatta ttttgtgggt agttgatgat gtactgtttc cagaaattgt
     2941 tcatgatttc gaagaatcaa aagcggttga gtttgaaata gtgaaaaaag taatggaatt
     3001 gattgaaaaa tacgaggagg acagcgaatg ataccgaagt ttagagcgtg ggataaacga
     3061 aagaacgtaa tgagagatgt agccgtcttg cattttacta aaaacggcaa aacaaacttt
     3121 attgaatatt ggataaatcc taccgaattg aaatcatatc acgtgcgaaa catcgacctc
     3181 atgcaatcca caggaatgaa agataagaat ggtgtggata tatttgaagg ggatatagta
     3241 ttagtcagcg tgcgaaatgg cttcgattac ttagataata aagtctgtat tgtcaaaaat
     3301 tcaatagatt attccggatt agtttgtgcc actgttgatg aagacttaga gtatcaaatt
     3361 tttaacacag agctgtttga agaatacacg tacgaagtca ccggaaatat atacgagaat
     3421 agcgagttat tggaggaaca gcgatgaata aacaggaagc aatcaaaaag ttagaaagta
     3481 ttaaagcgat aggaaatgat gcaatagctg cttgctataa cgagagtata aattcaggta
     3541 ttacgttaat gaaaaaaata gacgaaccgc agaaaccagt tattccacaa ctcgtggccg
     3601 gttggcttga gaaatctacg gaccctttta caaaagctga aaaaatagcg tatttaatca
     3661 aatctaaaga tggtgattca tattatttct gtgattggtt tgtacgagat ggcatagtga
     3721 cgcaagagca aggagaagaa ttactcgctt gggcaacaag acaatcatat gaaacactat
     3781 tgagcctata caacggctac gaggttgaga aagagccgtt atgggcaata aagaatgccg
     3841 atggaaacta tcttactaaa tgtgctttat ggggaaaaga tggagtaaat tatagttttg
     3901 aatgcaatcc atctcatcga ttgcttttca ctgataaagc aacagcggat gctgcagcat
     3961 tgttggtgaa tggaacagtg gaagaggtgg tagaaagatg aaactaaaag acggatttta
     4021 cgctagcagt cacggtatcg gcggtttaat gctagatatg ccgacaaaga accctaaaac
     4081 acgcaagaaa tcaaaattca aagtcggtga catggtccgc tgtgaagcag aagagttcgt
     4141 ttatccgttc agaggatacg ttaaaaagat actgtcaaac tcagcaatca ttcgtattga
     4201 aaatacaatg aagtgtgatc gttcgactgc aaaacacaag cattatttag ctgtagcgag
     4261 attagttgac atggaagtaa tcaagagcaa ataaaaaaag ccggatcgct ccgactgatt
     4321 caataaatcc aacacattta ttatatcaca taaaggagcg gtttgacttg atgcaattgt
     4381 tacgagaggt agatttcaaa cagacaagat gtaatgcgag agatgtgctg aagaactttc
     4441 ggcgtttgga gcggatggca ggtcgctctt tgatagatat taagtcgccg attataacgg
     4501 atatgccgaa ggcaccgaag catggcaata aggcagaaga cgcgattatt cagatgatgg
     4561 atatagaaac ggagagagat gcgattctag cggctttgat ggctcttagt ctgattagtc
     4621 gtcagatact ctactacagc ttctgtgtgc cagatagctt ctcaaactac agaattagcc
     4681 gtgaagtggg ttattcagaa agaagtatac aacggatgaa gtcggaagct ctaatagagt
     4741 ttgcagaagc atataaacac ggaaaaataa ttgcttataa ataatttggc ggttttttgg
     4801 cggaatgatg gcggttttta gacatttacc agtgttatta tggtagtgtc gaaagattag
     4861 gaaacaggat cgacaaaata aacgaaggga ggcaatctcc ctcatcgttt taaacttctt
     4921 cttagataga cagcaactaa ttatgaagaa aaggatgtga attcaactcc tcctaaattc
     4981 ttcatcggtt gctgtttatt ttgattagac agcagcactc acaaaactaa acatgttctt
     5041 gtcgtctttc aactgctgct gtctattaat ttatgtattg gagggaagaa aaatgaaatt
     5101 atcagtagaa ggcactccag aagaaataca agaattgctc caagctattg gtagtagcaa
     5161 ggagcaaaag gtaagtgttt ctaattcaga tattgacggt atttgcaatt gtttaaacag
     5221 agaaaaaata tctaatcctt actaagagaa aatatgttcg ttaatgaatt tccaattttc
     5281 ctctgtagct ctaccttgat aatttttttg cagttcacag atgaataagg aatcgttatt
     5341 atcaagtact gtatttaatt tttcacacat ttgatttgga ttcaaattag ttttaactaa
     5401 ccaaaaagat ttttgtagct ttatatatgc cccaaattct tttattattt caaatacttt
     5461 atcatacttt tgccctgggt tattaagatc ataactaatc atgtagggtt tatacatgtt
     5521 gtcttgcaca tccttgtttt atatttcagc ggaccactcg ctgataaata aaattatacg
     5581 cttagtattt attttcacaa tattaattta tcgtaataac ttttgagtaa atagaacaaa
     5641 aaacctgcac tagtttccgc tagtacaggc agtgactata tcggttgata atctagcata
     5701 tattaaaaat aattgcaaga aaagaaattt atagtggttt ataccaaatt atcaaagcaa
     5761 tgttattttg ttgctgtcta ttgtttttta attattcaca cgataactaa aggtgggtga
     5821 agagaaatga ttccattaat aatttcaatt tttgcgctct gtcttaatgt ctatatgatt
     5881 ggatttaaaa atgggcaaaa taaaaaatag tagcagccaa gaataatttt atagtgtcac
     5941 tgtggcggaa agggtagacg ctaagcatgt gtgctaggtc aatgcttcgg caaccatgca
     6001 aggttcgatt ccttgccagc gacattaaat gcctatgacg gttacgacta ccgaaaaaag
     6061 atcgttaaga agctatacgg tgctacgtac ggcaatgtag taagtgtgct atctgtacac
     6121 caccaagctt cggtcactgt ggcggaagta gaagacgcag cggtaaatgg cgagtagcct
     6181 cgtgagagcc tggtaagttc tcgtgagtgg tgcaatccca ctccagcgac tttaagcaac
     6241 cgaggcatcg gcggtttaaa aatatagggg tgcgcaattt cgtacgcgtt ttgtgcatcg
     6301 tgcaagttac tattacatat tagatcactc tttgagtggt ctttttattt ttgcacaaag
     6361 gaggaaacaa caatgtatag accacaatac ttagaacaga agtatgaagt aatcactgtt
     6421 cataaaggta atggcgaaaa agtatatgag tatagaagac caataaagag cgatacatat
     6481 aaacgaaagg aaaacaatga agttattcca ttgtatggca aaagaatagc taagcattaa
     6541 ataagattgc gaaaggagac ggaacatgac cgaggaattc tatagatggc tattacagtt
     6601 gacaagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca ggcttagaga
     6661 gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag gtaagtatca
     6721 tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact tagctttaga
     6781 tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg gcagatatct
     6841 tacagcatta gataaacaag agaagaagat agaaagcttt gctaacttcg atgcaagtga
     6901 aaggtggtaa gtgcatgatc atcaatga
//
LOCUS       DANKEF010000099         6900 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000099 DANKEF010000000
VERSION     DANKEF010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.100"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..442
                     /locus_tag="PWZ48_002663"
     CDS             <1..442
                     /locus_tag="PWZ48_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDL2262276.1"
                     /translation="EKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQREMRKV
                     EAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEGLVGKSM
                     TNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            442..1641
                     /locus_tag="PWZ48_002664"
     CDS             442..1641
                     /locus_tag="PWZ48_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2262277.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYKLL
                     PSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKFPN
                     SKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSAYYKNCMSII
                     NTNKLMEYDEFAIKHWREGGGTGGTITGSWGNPFPGSSLDKNSFSGGQLFGKNPGGEF
                     RPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVYQE
                     FANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFIDDGTWIDPLP
                     FLNSSKK"
     gene            1657..2301
                     /locus_tag="PWZ48_002665"
     CDS             1657..2301
                     /locus_tag="PWZ48_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262278.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTYKIEEVAK
                     NASDKLKKNSSEAQAKIDKLNQTINEKNQEIDQLKQQDGTKAKEEVTEAQRKTAEEFG
                     QLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            2312..3118
                     /locus_tag="PWZ48_002666"
     CDS             2312..3118
                     /locus_tag="PWZ48_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262279.1"
                     /translation="MSKKKGARQRFKKRKSYLRWLGIFMILGFLILASPIYTHKKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFVVKLAHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            3140..3370
                     /locus_tag="PWZ48_002667"
     CDS             3140..3370
                     /locus_tag="PWZ48_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262280.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            3884..4192
                     /locus_tag="PWZ48_002668"
     CDS             3884..4192
                     /locus_tag="PWZ48_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262281.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            4194..4616
                     /locus_tag="PWZ48_002669"
     CDS             4194..4616
                     /locus_tag="PWZ48_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262282.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            4636..6225
                     /locus_tag="PWZ48_002670"
     CDS             4636..6225
                     /locus_tag="PWZ48_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262283.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            6237..6572
                     /locus_tag="PWZ48_002671"
     CDS             6237..6572
                     /locus_tag="PWZ48_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262284.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLINEQF
                     DPNQFLVVEYPATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            6713..>6900
                     /locus_tag="PWZ48_002672"
     CDS             6713..>6900
                     /locus_tag="PWZ48_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262285.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         2646 a         1036 c         1419 g         1799 t
ORIGIN      
        1 agaaaaacaa atggaagaac aagacttcaa acgcttcttg gcgatgattg atgagtgcca
       61 taatgcgatt aatccagatt ttattgaaac ggtaaattat gtcacaaact ttcaacgaga
      121 aatgcgtaaa gtagaagcaa tgactgtttt agctacacaa tccccacaag aaatggtgcc
      181 agaaaatatg tcttctgata acttctcgaa actgaagaaa gtctttgaat tttcttctac
      241 gaagatcttt atgcgtatgg atgaatctat tttacgtcat atagaaggat tagtgggtaa
      301 atcaatgacg aactcagaat tagaatccat tcctcaacaa aatcaaggag acgctgtgat
      361 caattttggc tcaaaagaaa ccattcgtgt tcattttagt ccgaacaaac gtcaattaaa
      421 attgtttgat ggaggaaaat aatggaaaac caaaatgaaa gtttaatcaa acagtatgtt
      481 aagcgtcgtg caaagcggcg cttatttttg tgggtgtttg gtacttctgc aggtttgatt
      541 actatattga tcactgtgtt tgtgaccctt tttctaatac ttgcggcagg ttctattgat
      601 aattctgatt caagtagtgg tggcgaagct tttacaggag aatattcgga aggcttaccc
      661 atttataaag aaatcaaagg aagaggccct ttttctgatg agattgcgca atatgctgta
      721 ggtgcagcag tgaagtataa attattacca agtgtgatcc tttcgcaata cggttatgaa
      781 tcagcgtttg gtacgtcggc gtccgcaaga aatgatctca attattttgg gattacgtgg
      841 tttgatgggt gtctgtttcc taaaggaact gcgcgtggaa ttggtggcat tgaaggtggc
      901 tggtacatga agtttcccaa ctcaaaagct gccttctcct attacggctt tatggtagcc
      961 acacaatcaa attttaatgc ttgtgtcgga aataaaagcc ctggcgcaag cttattgatc
     1021 ttaggacgag gagggtatgc cgccgcagga attactgaag acagtgccta ttataaaaat
     1081 tgtatgtcta ttattaatac aaataaattg atggagtacg atgagtttgc gattaaacac
     1141 tggagagaag gcggcggaac tggtggtacg attacaggaa gctggggcaa tccattccca
     1201 ggaagtagct tagataaaaa ttcgttcagt ggaggacaac tttttgggaa aaatccagga
     1261 ggagaattcc gacctaacgg gttccatgat ggactagact ttggttcagt cgatcatcca
     1321 ggaagtgaga ttcatgcggt tcatggcgga aaagtcgtgt acgttggcaa tcctggtatt
     1381 tcaggtttgg gtgcgtgtgt gatcgtgatt aattacgatg gcttgaatat ggtgtatcaa
     1441 gaatttgcaa atagtacagg aaattctcga gtgaaagtag gcgatcaagt gaaagttggt
     1501 caagtaattg ggatacgaga tacagcacac ttgcatttag gatttactcg aatggattgg
     1561 cgtcaagcac aaggacatgc attcattgat gatggtacat ggatcgatcc gttaccattt
     1621 ttgaatagca gcaaaaaata gatcaggaga aaaaatatgg aaaataaaac cttaaaaatc
     1681 gtcttaacag ctgcaattag cgttagttta atcggcaata ttgcgttgtt ttggcagctt
     1741 aaagaagaac aagagacaaa tacctataaa attgaagaag tggctaaaaa cgcgtctgat
     1801 aagctcaaaa aaaattcttc tgaggcacag gcgaagatcg acaagttaaa tcaaaccatc
     1861 aatgagaaaa accaagaaat cgatcagctg aagcaacaag atggcacaaa agcaaaagag
     1921 gaagtaactg aagcacaaag aaagacggca gaagagttcg gtcaattggc cattgataaa
     1981 tcattaagca gaaatgaatt aacggaaaaa ctaaaagatg tcgcaaccca agaagtgatc
     2041 gacaaattaa gtcctgcaga cgatgatcac caacatgacg actacggaag ttactctttt
     2101 acattaggtg acgtgcaaac ttacgctcaa acaagcagtg taaaagagga acaaaatttt
     2161 gtcgtatttg ttgattatac aatcggcaat cctcaattta aagatgtcaa accacaaaaa
     2221 ataaaaggtg gattaactgt cactgaaaaa caagtagatg gtcaatggaa agtcacagac
     2281 tttagttatt tcactcgtta ggaggaagaa aatgagtaag aaaaaaggag caagacaacg
     2341 ttttaaaaaa cgaaaaagtt atcttagatg gctggggatc tttatgattc tcggcttttt
     2401 gattcttgct agtccgattt acacacataa aaaggtagac ataccagatg aagtaagcac
     2461 gaacgcattt ttaaacacga aggaactgac gatggtttca aaagaaaaag gagcgaacca
     2521 gcaattagtt tttgaatttg cgatcgacga tgaagatcag agcttattaa aagaactagc
     2581 taatcttaat tatcgtgtag aagtaaagac agctaaaggg gattatcaag caataaaagc
     2641 caaagtgatc aaggtgtctg atgattattt tgtagtgaag ttagcacatg taccagaaga
     2701 atatatcgcc atgcgtttaa ccattattcc tgaaaaaatt gatccaaaag tggatatgca
     2761 agaaccacaa gatctgattt attacgttca cgaagataag gttaaagatc aagtgaaaga
     2821 tgaagattat gaacaacatg cgatcaatta caaagtgaaa ggatacaaaa aagaacaaaa
     2881 agcagcacaa aaacaaatgg aatccttcca agcaaccatt gatttgaatg aagaattagt
     2941 gaagaagcta aaagatcaat tgccttatca agtcgcagat gatcaggaaa atactgaaaa
     3001 caagattaat agctaccagc aagaaattga aacatcgaaa acgcaaatga aagatcaaga
     3061 agagatcatt caaaaacttc aagaaaaaat tgatcgtatg acaaaagaaa agatttagaa
     3121 ggacaggtgg agtgaaaaaa tgaagcgaac aaacttggct gacaaagata ctttcgctga
     3181 gcagaaagta ttgattaaag cctatctcaa agttgtaaaa gctaaagaaa tatgtcgaga
     3241 agtgaattta tctaatacaa caatatctga gttgaagaat ggtcgaaaga atttagatac
     3301 tttaaaattc agatcatttg aagctctata tgtagctgct aaaaaatatt ttacagctga
     3361 taacaaataa aaaacaggag tattttatgt gaaaaaagta tataaaaaat gcttatgaat
     3421 cagctatatc accttaacca tttgttaatg agatgggaga aaacattaac cacgattcta
     3481 acgcaaacat caagcacata aaacatttaa cactcctcct ttctggctga agatttcaac
     3541 caaacaggaa gacagcgtat caattatagc atacactttt tcttaaattc atttttcata
     3601 tgacaaaata tttccttcta atcgacaaag ttagaaggat tttttattgt ctaaatacac
     3661 aaataacctt aatttataaa gagcataagg aaacgtggga acacttttta aaaaaagtgt
     3721 tccaataggc tgtgcctatt acgtttctca aaaataattg tgggaacaaa atatttaaaa
     3781 ataggatagt gaaaggatag caaaggatag taaaaggata gaagcaggat agcgaaaagg
     3841 ataggctcct tttcgctatc ctttttaaat tggaggaatt aatatggaag taagaataag
     3901 aaatgtagat gagaaaatcg taagagaaat tgatcgaaga tgtgacgaac taagtaaccg
     3961 aacgggtaat aaatggagtc gtaacgatta tttaaagcta ctcgttgaaa atgattttga
     4021 tcgtcctttg atggaatata aacaagataa atttgatcaa ttattaaatc agttaataga
     4081 taaattttct tttgttcaaa gtcataatac aaaagtatta gaagaatata tttgtcagaa
     4141 taatcaaatt atcgaacttt taattgaaca aagtagagga ggagaagttt aacatggcca
     4201 aagttataga caagcatatt tatttttatg aaacacatat agatgttcta gatgaaatta
     4261 ttgcaactca tgcagaggtt aaaaactata gtgaagccgt tcggtttcta tgtatgaatt
     4321 atgattcaga agtggagaaa aaagtaaatt ccaatcaagc aaagttgaat gcaatgggaa
     4381 aagaaatttc tattttaacc gaaatggttt cagagtttgc ggatctccat ttgaaagata
     4441 cagggatttt aattggtaca aattcaactg tttaccaaga agcaaaagag cgtgtggaag
     4501 gcaagataaa agcaaatcaa acgaagaaat attctcctaa gataaagaaa acaataagcg
     4561 aatcagtgac tacaaaaagt gaagtatttg atccaacaag gttcttaaaa cgataaaaat
     4621 tagaaggaga atgtgatgag taaatcacct gcagtcattc tcacttgtca atttaccttg
     4681 cccaataaaa aatctttttc aacatacatt gattatatga caagagaaaa agcgctggaa
     4741 gaaaaggaac gacgtacgcc cgaggaagaa caggaactgc gtctaatcaa gaatgcttta
     4801 gaggattttt atattcctga aggagaaact ttttcggaga gtaagacggg taaagaattc
     4861 agcgaaacgt cgaatgaagc gcaatcgttg atgaaacatg gaatcaattt tgacaagtta
     4921 gaagaacaag attttaccaa atatctttct tatatgacac gtcactatgc gttagaacaa
     4981 aagaaagtac tgacgcatag tgaacaaaaa gaataccaac gcttgaacca agcaataaaa
     5041 aaatatcaaa cagagccaca caaaaaagct tcaggaaatc ttcctggagt tttttctatg
     5101 gcttcagacg aagtgaaagg aagtgatctg aaggagatca agcaaacctt ccaaagagga
     5161 caacaaaaag gctcaatcat ttatcaagat gttgtctctc atgatcattc gtatcttgaa
     5221 aaaataggat tgtacgatcc caaaacagat actttagatg aagaaggttt aaaagcagct
     5281 ggaagaaaaa tgatggagac attgttcgag aaagagcaac tgaatgaaac aggctactgg
     5341 atggcaacga ttcaccgaaa taccaaacat atccatattc attttgcagt tgttgaaaaa
     5401 aaaaatacgc gtaaaccata cactgagatt gaaaatggtg tgagttatat cgtaccgaaa
     5461 gggaagagaa ggcaagcaac attagacgaa atgaagacta attatgtaca tgagttagaa
     5521 cgtttttctt atgaaaaaaa tcgaatgggt atggagaaac gaaatttatt gacacggaaa
     5581 agtgatttgc gtaacacctt aaccaaagta gtgaaagagc cagtacgtta tgatcgacac
     5641 gcaatgagct tattaactga agtatataaa aacttaccta aaaagcgttc ggaatggcat
     5701 tacggggatg aaaatcgaac caaactttca ccatacacaa gagagaaact cgatgagtta
     5761 acccaatatg tgttaacaaa agaacctgga tatcaagaat atatacagat ttcaaaagca
     5821 ttgaaagaag aagcaaaaag tctttatggg gaaagcaaga gagaatcaaa ggatgcagaa
     5881 aaaaatgctc tctttgattt aaaaaagcgc acaggaaatg ccattctaac agaactgaag
     5941 aaacaggata gtcaaataaa acctttaata gaaaggatgg atcagatgca atctatttac
     6001 aatgaacgat cctttcgaac acttaaacag ttgccaacga atgaatacaa atatagttac
     6061 gaagaattca tgaagcgcag gaaacaattc caacaaagag ttgtcacgca acggagtttg
     6121 cgtcaattaa atcgaacgat taatgcccat gaagaaaaat accacgcaag taaagcatac
     6181 gaagagcaac aacgaagaat tcaaatggaa caagaaagac agtaggagga atagcaatga
     6241 aacaatattt agatcaatgg aaagttatag aaggatcatt aagagaagaa cgaatcgaac
     6301 aacttcccga ttgtttagaa aaagagcatt tgtttcagat aagagaaatg ttgataaacg
     6361 aacagtttga tccaaatcaa tttttagtcg ttgagtaccc agctactggt gtgtactgtt
     6421 gtaatcatgt gaagggtgaa aaatatttta tcattcaaga atatgagggc aagttagcgc
     6481 cttattatac tacttgggaa atgaatgaag aggggatcaa taacttcccg tgtaaaagca
     6541 ttgaagagtc tatttcactc acagaatgtt agttgttaag tgaataaaag aagtaaaaga
     6601 gtcctgaacg tgattgtcca ggactctttt ttttcactga attgttttcc gccaattcat
     6661 tgagtggtgc gatttccgtc tacattatat caaaagaaag gaggaagaag aaatggctta
     6721 ttcaacgatt ggagttgttg ttgaaaaaag cagagacaat cttgtttttg tgacagaaat
     6781 tcaaacaggc agagcttttg tagtcacaga caaagcggca aaagcatatc aaagtggcga
     6841 tattttaacg ttaaatctga ctacgaagac gttcgtcgat gcggcagaag attatccatt
//
LOCUS       DANKEF010000100         6869 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000100 DANKEF010000000
VERSION     DANKEF010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6869
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.101"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..227
                     /locus_tag="PWZ48_002673"
     CDS             <1..227
                     /locus_tag="PWZ48_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HDL2262286.1"
                     /translation="KTTEFDILHALMSTPNKVFSRSDLIEKVKGIEFDGLDRSIDSHI
                     KNLRHKIENDAKSPMYILTVHGTGYRFGGH"
     gene            227..574
                     /locus_tag="PWZ48_002674"
     CDS             227..574
                     /locus_tag="PWZ48_002674"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262287.1"
                     /translation="MRKRTIKTQLAVSFLAIATLIIGSISLVALSLMNNHFSKYVEER
                     QEDLLNQYVYTIDLLWLNSGETWNSEELAALSEKVLENNIYFSIEDEQGNMVWELTGK
                     DLKSAQEKLKKMH"
     gene            571..1620
                     /locus_tag="PWZ48_002675"
     CDS             571..1620
                     /locus_tag="PWZ48_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2262288.1"
                     /translation="MKVSEKNSVKLDETIEVKKKLINDGNEFGKVTFYYFGPFAYTEH
                     DALFISSMKQSLMYVAIAALLVSFILASWISARLGLPLKHVSDFTHKLTRGEYADKIP
                     QETSIIEINSLIDSLNDLSNQLEKQHGLRKRLTTDISHELRTPLATLKGNVEGMIDGV
                     WKITPERLQSCYDEIDRLTRLIGNIEIINKIEAKYDHLNKTEFNIYKLIESVIENFAS
                     KIESKNLHVEIQGDNINISADKDKMNQVVINLLNNAIKFTQKEGTIKFSISKNKDHVL
                     LIVEDNGIGIEKDQQLHIFDRFYMADPSRSRALGGQGIGLAIVKSVIEAHKGSITVKS
                     KLGLGTKFVIKLPFQ"
     gene            1635..1841
                     /locus_tag="PWZ48_002676"
     CDS             1635..1841
                     /locus_tag="PWZ48_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262289.1"
                     /translation="MFQKQIKIAKLVCECWAKKIFFQLISIDSVEKVHLDILSNSVVI
                     FSKKDLSIATIKKEVFGLQIYEMT"
     gene            1862..2458
                     /locus_tag="PWZ48_002677"
     CDS             1862..2458
                     /locus_tag="PWZ48_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhK family protein"
                     /protein_id="HDL2262290.1"
                     /translation="MKTVKKMTKLVFASLGIIVFLGACSNQSESNNSNSTNEESTSTA
                     SSEMNSMEGMNHEGMVPSSMKDAANPKFPVGSNVILLGDHMEGMKGANAQVVGAFDTT
                     MYEVSYEPKTGGPMVNNHRWVVQEELKDTETVANEGDTVILNADHMDGMMGAEAKVDK
                     SITGTVYVVNYTPTDGQEEVKNHMWVTEDEMEYDENNE"
     gene            3410..4009
                     /locus_tag="PWZ48_002678"
     CDS             3410..4009
                     /locus_tag="PWZ48_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2262291.1"
                     /translation="MKSVEPIRDKKKIDAMKAILASGKYGQRNLVLFSIGINTAYRIS
                     DLRQLKLRDVLEISRGRVIVKERLAMKEQKTAKHNSVFISNKLRKVILDYVQSEFPEQ
                     LQAQDFSKYLFPSRKGADTPLTRQSLWRIIHEAGTAVGLKEIGPHSMRKTFGYFLYKQ
                     GTKTEIIQSLLNHSSQRETLRYIGITQEDKDTAVKSLDL"
     gene            4058..4774
                     /locus_tag="PWZ48_002679"
     CDS             4058..4774
                     /locus_tag="PWZ48_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HDL2262292.1"
                     /translation="MNEEKQPNFPDKYHLSRKESVYLLKKNIVELVYNAGKFEGLNTT
                     LLQTEGIIKYNRANNVAVDDVLTVVNLKRGFEMLLNDVQEPLLETSKRINRIVAAEDA
                     LFPGEIRTGGVELSTIQGRYVPPMLTEDEVNNQYGEIMNQEISDTEKALRLFLFISKN
                     QIFWNGNVGTALLIANKIMFSNGLGLLSIPENVFMKFNELLSKYCNSNLLVNESNIIS
                     FLYEQCIFGINYLKIIQHTI"
     gene            4788..5567
                     /locus_tag="PWZ48_002680"
     CDS             4788..5567
                     /locus_tag="PWZ48_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002396205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HDL2262293.1"
                     /translation="MMTIDEAKQLATYFSVGSAGYILDFTNVTWNIFTKKIVGIEIQN
                     HYGGSKGVSFQKFLLDDQFDDSLKNRLIQSLKGIMDRDKKYESTEMNPIVQLLFEEVF
                     KEDTSTALRKMKFSSEIDLLYIRQLPIRIQTDIKNKDFDSVLTKANTLIDEVLKFIIE
                     NTEGPSVNINLFKSKELRKEVFGRLNIKTEKDMDNRIKALVGALNTLSDKILEMRNSQ
                     GDAHAHGSNRIVIKEEEAILVANSSMVLCEYVFTKYKGLNR"
     gene            5650..6006
                     /locus_tag="PWZ48_002681"
     CDS             5650..6006
                     /locus_tag="PWZ48_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2262294.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVDNGRYVITHGFTKKTEKTPITE
                     LKHAKELREEWFTNDD"
     gene            5996..6262
                     /locus_tag="PWZ48_002682"
     CDS             5996..6262
                     /locus_tag="PWZ48_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2262295.1"
                     /translation="MMIKKLIEEKKKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            6421..6699
                     /locus_tag="PWZ48_002683"
     CDS             6421..6699
                     /locus_tag="PWZ48_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262296.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYVNNSSGQSVINKQIVENRVLLQSFKNKISFCIISSF"
     gene            complement(6696..>6869)
                     /locus_tag="PWZ48_002684"
     CDS             complement(6696..>6869)
                     /locus_tag="PWZ48_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010868911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2262297.1"
                     /translation="HRSIKRRHKLYQSLRTASTTIKGMETIRGIYKKSRKEGTLFGFS
                     VCTEIRILLGIPA"
BASE COUNT         2527 a          957 c         1333 g         2052 t
ORIGIN      
        1 ttaaaacaac tgaatttgat attctacacg ctctaatgtc cacacctaat aaagtctttt
       61 ctcgtagtga tttgatagag aaagttaagg gaatagaatt tgatggacta gataggagca
      121 tcgattctca tataaaaaac ttaagacata aaattgagaa tgatgctaaa tcacctatgt
      181 atattttgac agtccatggt acaggataca gatttggtgg ccactaatga gaaaaagaac
      241 gataaaaact caattagctg tttccttttt agcaatcgca acattaataa taggatcgat
      301 tagtttagtc gcattaagtt tgatgaacaa tcattttagt aaatacgtgg aagagcggca
      361 agaagatttg ttgaatcagt atgtatatac aattgattta ttgtggttaa atagtgggga
      421 aacatggaat agtgaagaac ttgcagcact atctgagaaa gtattagaga ataatattta
      481 tttttctata gaagacgaac aaggcaacat ggtttgggaa cttactggaa aagatttgaa
      541 gtcagcacag gagaaactga aaaaaatgca ttgaaagttt ctgagaaaaa cagtgtgaaa
      601 ctagacgaaa caatagaagt gaaaaagaag ttaattaatg atggaaacga gtttggaaaa
      661 gtaacgtttt attatttcgg accttttgct tatacagagc acgatgcctt gtttatctcc
      721 agtatgaaac aaagtttaat gtatgttgca attgcagctc ttcttgtatc attcatactt
      781 gcttcgtgga tctctgctag attaggattg ccactaaagc atgtaagcga ttttacacat
      841 aaattaactc gtggggaata tgctgataaa atcccacaag aaacttcaat tattgaaata
      901 aattccttaa ttgattccct aaatgattta agtaatcagt tggagaaaca gcatgggctt
      961 cgaaaaagat taactaccga tatttcacat gaattaagaa ctccattagc tacgcttaaa
     1021 ggcaatgtgg aaggaatgat tgacggagtc tggaaaataa ctcccgagcg cctacaatct
     1081 tgttatgacg aaatagatcg tctaactcgt ttaattggaa atatagaaat aatcaacaaa
     1141 attgaagcaa aatatgatca tttaaataaa actgagttta acatatataa gttgattgaa
     1201 tccgttattg aaaatttcgc cagcaaaatt gagtcaaaaa accttcatgt tgaaatccaa
     1261 ggggataaca tcaatatatc tgcagataaa gataaaatga accaagttgt tatcaatctt
     1321 ttgaataatg ctattaaatt tacgcaaaag gagggaacca ttaagttttc tataagtaaa
     1381 aataaagacc atgttctttt aattgttgaa gacaacggca tcggtataga aaaagatcag
     1441 caacttcata tttttgatag attttatatg gctgacccct ccagaagtag agctttaggg
     1501 gggcaaggta tcggattagc tattgtaaag agcgttattg aagcgcataa aggtagtata
     1561 actgtaaaaa gtaaattagg tctaggaact aaatttgtta taaagttacc atttcaataa
     1621 aggaggagag ttatttgttt caaaaacaga taaaaattgc taaactagta tgtgaatgtt
     1681 gggcaaaaaa aatatttttt caactgattt cgatagatag tgtcgaaaaa gttcatttag
     1741 atatcttatc aaattctgta gtaatctttt ctaagaagga cttatcaata gctacaatta
     1801 aaaaagaagt ttttggatta caaatttatg agatgaccta aatgtgatta attaaaggag
     1861 gatgaagaca gtgaaaaaga tgacaaaatt agtatttgca agtcttggaa ttattgtatt
     1921 tttaggagcg tgtagcaatc aaagtgaatc gaataattct aatagcacaa acgaggaatc
     1981 gacttctaca gcgtctagtg aaatgaatag tatggaggga atgaatcacg aaggtatggt
     2041 tccttcttca atgaaagatg ctgcgaaccc taaattcccg gtgggaagta atgttatact
     2101 gctaggcgat catatggaag ggatgaaagg tgcaaatgct caagttgtag gggcctttga
     2161 tactacaatg tatgaagtaa gctatgagcc aaaaactggg ggaccaatgg ttaacaatca
     2221 tcgatgggta gtacaagaag agttaaaaga tactgagact gtagcaaatg agggcgatac
     2281 tgttatcttg aatgccgatc acatggatgg gatgatggga gctgaagcaa aagtagataa
     2341 atctattaca ggaactgttt acgttgttaa ttacactcca accgatggtc aagaagaagt
     2401 taagaatcat atgtgggtaa ctgaagatga gatggaatat gacgaaaata atgagtaatt
     2461 ttatatacta aaccaacaag ctggtacatg gtcttcgtat tctcaacatg aggaatatga
     2521 gctctttgtt aataaggact gatgagttgt gcaaaatcaa atctgggtca gaacgaacct
     2581 cattctggct cagatttttg gctcttcgac atttagtgct ggtagagaga caactgaaaa
     2641 tttagtcata accagttagg cagagtgctt aactggtttt tgttcgcatt tattattctt
     2701 ttgatattgg ctgcttgccc ttcaatttta tttggtttct taatcaactc ttcatataac
     2761 cccaaatcat atattaatga ggtgcctcct attcctataa atctatctct tctttaatag
     2821 tatgagaaaa gtctactctt gtagatttaa attgtcaaca aaaaatttac agatgtaaat
     2881 gataagttga atagagaaag ataaattatt tttttggtat tcatataagg ttaattatgt
     2941 attatcacaa aatgattgtt gtcgaataac taaaagtgtc aaaacaatga tgaaatcatt
     3001 tagttcttat aaattagaca agagaaaata aatattccat atctacttta ttggatccat
     3061 aacatgacta aattctgaat actttttaaa gcttatcatt tggtaaaact gaatataagt
     3121 taattatata gactgaaaat tttgaatttt cagaatagaa taacttaaaa gtactgacaa
     3181 attttttaaa ggggcaatat taatgttctg agataatcaa atcttaccga aaatatcaaa
     3241 agtccaatgt gacacaattg gattttgtca cattcgttaa aatcgctcca tccttagagg
     3301 ctctaatagg aaatcacttt tcttttagcg ccttttttct atagcttgtt taaatgtgac
     3361 ttattatctg ttataatcgt attaagtaac atttgagagg tgtctaaaaa tgaaaagcgt
     3421 ggaaccgatt cgcgacaaaa aaaagatcga tgccatgaag gccattttgg cttccggaaa
     3481 atatgggcaa cgaaatttgg tgcttttttc gatcgggatc aatacagcct atcgaatctc
     3541 cgatttaaga cagttaaaat taagggatgt gctagaaatt tcccgtgggc gggtgatcgt
     3601 caaagaacgt ctggccatga aggaacaaaa aacggccaaa cacaattcgg tcttcatttc
     3661 gaacaaattg cgaaaagtga ttcttgatta tgttcaatcc gaatttcctg agcagttgca
     3721 agcacaagac ttcagcaagt atttgtttcc aagtcgaaaa ggagcagata cgcctttgac
     3781 ccgacaaagc ctttggcgga tcattcatga agcaggaact gcagttgggt taaaagaaat
     3841 tggtccccat tcgatgcgta agacctttgg ctatttttta tacaagcaag gaaccaaaac
     3901 agagatcatt cagtcgctgc taaaccattc ttcacaacgg gagaccttac gctatattgg
     3961 aatcacccag gaagacaaag atactgcagt aaagagtttg gatttatgac agaataatgc
     4021 cctatataat agaaggaaaa attcaagagg tgagaaaatg aacgaagaaa agcagcctaa
     4081 ctttcctgat aaatatcact tatctcgcaa agagtctgtg tatttattga agaaaaacat
     4141 tgtagaactg gtgtataatg cggggaaatt tgaagggtta aatacaacgt tgttacaaac
     4201 agaagggatt attaagtata atcgggccaa caacgtggct gtagatgatg tcctgaccgt
     4261 cgttaactta aaaagaggct ttgaaatgct tttaaatgac gtacaggagc ccctgttaga
     4321 gacaagtaag cggattaatc ggatcgttgc agcagaggat gcgctttttc ccggtgaaat
     4381 acgaaccggt ggggtagaat tatcgacgat tcaaggaaga tatgtgccac caatgttaac
     4441 tgaagacgag gtaaacaatc aatatggtga gataatgaat caagagatat cagatacgga
     4501 aaaagccctg cgactgtttc tattcatttc gaaaaatcaa atattctgga atggtaatgt
     4561 gggtacagcc ttgttgattg cgaataagat tatgtttagc aacggtttag gactattatc
     4621 aatacccgaa aatgtattta tgaaatttaa cgaactttta tcaaagtatt gcaacagtaa
     4681 tctattagtc aatgagtcaa atattatatc atttttatat gaacagtgta tctttggtat
     4741 taattatttg aaaataatcc aacatacaat ttaagaggta taagtaaatg atgactatag
     4801 acgaagcaaa acaattagca acttattttt cagttggttc agcgggatat attttagact
     4861 ttactaatgt aacgtggaac atatttacaa agaaaatcgt agggattgaa atacaaaatc
     4921 attatggggg ctcaaaggga gtatcctttc aaaagtttct gcttgacgat caatttgatg
     4981 attcgttaaa gaatcggtta atccaatctt taaaaggtat tatggatcga gataaaaaat
     5041 atgaatctac tgaaatgaat cccatagttc agttgctttt tgaggaagta tttaaagaag
     5101 atacttcaac tgcattaaga aaaatgaaat tttcaagcga gattgattta ctttatattc
     5161 ggcagctacc cattaggatt caaacagata ttaaaaacaa ggattttgac tctgttttga
     5221 caaaagcaaa tactttaata gatgaagttc taaaatttat tattgaaaat accgaaggac
     5281 cgagtgttaa tattaattta tttaagtcaa aagaattaag aaaagaagta tttggccgcc
     5341 tcaatattaa aactgaaaaa gatatggata atagaatcaa agcccttgtt ggagcactca
     5401 atacattatc agataaaata ctcgaaatga gaaatagtca aggagatgct catgcacatg
     5461 gctcaaatag aatagtaatt aaggaagaag aagcaattct tgttgcaaat tcttctatgg
     5521 ttttgtgcga gtatgtgttt actaagtata aaggattaaa tagataataa aatgcatatt
     5581 ttatagtata caattcgaat ttttatgtta tatactataa tataaaatca atatatgata
     5641 taattgggta tggaaaaaat atcttttgaa acatttaaaa gacccaatgg tcatgatgaa
     5701 tttttagaat ggttggaaac attaccaaaa aaagatagtg cgaagctttt acgtacaata
     5761 gaagaaacag aaaagaatgg gatgctagta gctcaacggt tgaaatgggt gaaaaaatta
     5821 gacactaatt tgtacgaatt aagaagtaag gtaggatcaa acatccaaag agccatctat
     5881 ttccatgtgg acaatggaag gtatgtcatt actcacggat ttactaaaaa aacggagaaa
     5941 acaccaataa ctgaactcaa gcatgcaaaa gaacttagag aggagtggtt cacaaatgat
     6001 gattaaaaaa ttgattgaag aaaagaaaaa agataaagat tttgcagaag catatgaccg
     6061 tgaaggaaga aaactcgaaa ctgcggttgc tctttatcat gctcgggagg aagcagggct
     6121 aactcaagcg gagcttgcac aaaaagcaca tacaactcaa tctactattg ctcgaattga
     6181 gagaggggat aacgtatctt tcgataaact tgatgatatc gccagggcat taggtaaaaa
     6241 gctggtagta gaatttgctt aaaagcgagt tgaaaatata tctataatag gctcctaact
     6301 aatcaattag tttagtgggc ctttttttac tagatagata aaggttgtac tatagtgtaa
     6361 aagacaataa attttatact aaaattactt taaagagaac tagagagaaa ggtacaataa
     6421 atgaaaacca taaatcttat aaaagtattt atagcatcac caggtgatat agcagcaaaa
     6481 aggaatgaag tagaagaaac catatataga tggaatatag aaaatacaga taatacaaaa
     6541 gtgattttga tgcctatacg atgggaaact aatgcgattc ctacttatgt aaataatagc
     6601 tctgggcaaa gtgttattaa taaacaaatt gtagaaaata gggttctgct gcaaagtttt
     6661 aaaaataaaa tcagtttttg tattatttct tcattttaag ctggaattcc caataatatc
     6721 ctgatttcag tgcagactga aaagccgaag agagtgcctt cttttcggct tttcttgtag
     6781 attcctcgaa tggtttccat ccctttaatt gtggtggaag ctgtacgtag actttgatac
     6841 aatttatgtc gacgttttat cgatcgatg
//
LOCUS       DANKEF010000101         6828 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000101 DANKEF010000000
VERSION     DANKEF010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6828
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.102"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            80..1552
                     /locus_tag="PWZ48_002685"
     CDS             80..1552
                     /locus_tag="PWZ48_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2262298.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            1635..2423
                     /locus_tag="PWZ48_002686"
     CDS             1635..2423
                     /locus_tag="PWZ48_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDL2262299.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(2490..3158)
                     /locus_tag="PWZ48_002687"
     CDS             complement(2490..3158)
                     /locus_tag="PWZ48_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HDL2262300.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            3273..4985
                     /locus_tag="PWZ48_002688"
     CDS             3273..4985
                     /locus_tag="PWZ48_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2262301.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            4988..6760
                     /locus_tag="PWZ48_002689"
     CDS             4988..6760
                     /locus_tag="PWZ48_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2262302.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
BASE COUNT         2081 a         1147 c         1482 g         2118 t
ORIGIN      
        1 tatggcagtt aacctcttga aaaagatagt aattatttat ctatttttag atcctgtcat
       61 aatgaaaggg aggaataaga tgaaaaaaga aacaaatgtt actttggtca cagtgagtat
      121 tttcattgca acgttcatga cggctattga aggtacaatc gtaacaaccg ctatgccaac
      181 gattgtcggt tcattacatg gaatggaaat catgaactgg gtattttcga tttatttgct
      241 gacaaacgct atgcttactc cgatttacgg aaaacttgcg gataaagtgg gacggaaacc
      301 aatctttatt tttgggacat tattgtttat tatcggttcg ttattttgcg gtttatctca
      361 aagcatgatt gggttgattg ctgctcgtgc gattcaaggg atgggtgctg gggcaatgat
      421 gccagtagct ttgacgatca tcggcgatct ttattctatt gaaaagcgag caaaagtttt
      481 aggtttgaat agttcagcct gggggattgc cagtgttttt ggaccgcttg cgggaggatt
      541 catcgttgaa acgatcagct ggcactggat cttttttatc aatgtgccga tcggaattct
      601 attaattatt ttgatttggc tatatttaaa tgaaccaaaa atcatacatg agtctaaacc
      661 aatggatatt tttggtagtt ttagtttgat ggccatgctt gtctcattgc tgttagggtt
      721 ccaattatta ggagacgaag gaatgagtcc gcttgtttct gctttgctgc taggtagtat
      781 cgtaatgtta ggtatttttg tttttgccga aaaaagagct aaagatcctg tcatcatgat
      841 gcatttattc aaaaatccac tatttgttgt ggtcaacttg gctgcagcat tggctagtgg
      901 ttttttgatg ggaatcgatg tttatatccc tatgtggatg caaggtgtat tagggctgaa
      961 tgcagcaatt ggtgggctgg tattagcacc attatcacta gtctggatgg tcggttcttt
     1021 ccttgcaagc aagtggatga ctcaaatgaa aactcattgg gtcttgagaa ttggactact
     1081 aatcgcagct attgggggcg cttggttgta tcttatgcct ttcagaagta actatttatg
     1141 gttttttgct atatcaacgg tcatcggtat aggcttaggt gtgaccatga ctacctcgac
     1201 tgtcagtgca caaaatagtg tatctcgtga ccagcttggt gtagcaactt cttttaatac
     1261 gttagttcga acaattggtc aaacagtcat ggtttctatt tttggtttat tgataaatgg
     1321 ggtaactaaa cgagaacttg cagcagcaaa tttaacaaat gatgcagata tcatgaataa
     1381 actggtgaat ccacaaacgg ctaaatatat tgatgcggat attttgttac cattaagaaa
     1441 aattctttat gatgggttac attccgttta tctagttggc ttgttactcg tgatcattgc
     1501 tttggtctta acgcttttcg ttcatgaaaa aagtggtaaa ataaaacagt agtttcggtt
     1561 aagatatgta cgtagccgaa tcattcaaat ttaaagttat tcaaaaataa cttcttacta
     1621 aggaaggcga aaatatggca tttattcaag caaatattta ttcgaatgtg ttagaaatgg
     1681 aagtcaatct aaatgtgata ctaccgcaaa caacaaaaaa ggtgattggc accagttcag
     1741 agcaagagac aacggatata cctgttttat atttacttca tggaatgggt ggcaatcata
     1801 gtgtatggga aagacgtacg tcgattgaac gttatgtagc aaactacgga ttagctgtca
     1861 tcatgccatc tactgatctt ggctggtaca cagatacagt ttatgacatg aagtattgga
     1921 cattcattgc agaagagcta cctgagattt gtcatgaatt attcccgcaa ttaacaagaa
     1981 aaagagaaaa aacatatgcc gctggattat cgatgggcgg atatggcgcg ttaaaactag
     2041 gccttgcaaa atcagaaaat ttcgcagcag taatctctct ttcaggcgct gtttctgtag
     2101 gaaatcgaat ggatgatctt ttgatggttc gtaaagcatc tttttgggaa gggatctttg
     2161 gaccattgga tcaaatcgaa ggttcaaaaa atgatccgca atacttattg aaagaactag
     2221 taaaaagcgg aaaaccagtt ccaagatttt atatggcttg tggggaatct gattttcttt
     2281 atcaggcaaa tcaggaaatg gtatctgcta tgcaggaaca aggcttggcg gtcacctttg
     2341 aacaaggacc tggcgatcat aactggatct tctgggacca atggattcaa cgtgcattgg
     2401 cgtggctaat ggaagaaaaa taaaaaaaga gtcttaaaaa gatagctcct atagagatca
     2461 caaacatagt gatctctata ggagcttttt tactgctcgt tttctcttct tttattttgt
     2521 tggcataagc cttcaatata ggtttgtgca ctcgttagaa agagtgcttt ttcattcaat
     2581 tctgcatggg actgcatact ttctttaaat ccctctaatt gattttcaac tacataatta
     2641 cgcataaata gaactgtttc tttcaaaaat tcatttccgt ccataatttt ttgtcgaagc
     2701 tgctgttcat gctgctgatc cattaactgt ccaatcaata aattgtggag agaggcaatg
     2761 gcctgatgac tttgggtgac tgaaaatcct tcacgaacaa gtaattggat catttgctcc
     2821 aaatgttcta atcgactcgg aaaagcgggc acagtattga tttctaattc tgccccacaa
     2881 gggaaacgtg tataaaggtc atagtaattt tccatatact gcagcaactg ttcattccaa
     2941 ggcaaagttg gatctggaag cgctaattct tcttccattg tttcagccat tttttgcaag
     3001 agctgttgtt tgtttttgaa ataccaatat aaagcaggtg cttgaacatg aattctgttt
     3061 gcgatgtttc gcatggaaag ctgcgaaaga gcttgtgttt cagccaggat atcaaaggcg
     3121 gcctgaatga tgattgcttg agaaagcttt atttccattt tatcaacctc gtgtattgca
     3181 ttatcttttt aaacatggta aattgttagt cggtctaatt ttacagtgtt aaattagtcc
     3241 tgtcaattta aacaaagggg tgtaatcaac gaatgaaatt aatcttacga catgcgaaaa
     3301 aatataaagt agctgttttt atttccttgc tgtccgtagc agtcatggtt gcagcagcat
     3361 tatggcaacc taagttacta cagcaagtat tggaagctat tattacagaa gataatgatg
     3421 aaatgaaaac aattggtatt tacctgatca gtctggcttt gctaggatta gccgcaggtg
     3481 taacgaacac gattttctct gcaaaagtag ctcaaggcgt aagtgcagat atccgagaag
     3541 aagcatttcg aaagatccag acattttcat ttggaaatat cgaacagttc tctgctggaa
     3601 atcttgttgt tcgtctaaca aatgatatca cgcaaatcca aaatttagtg atgatttctt
     3661 tgcaatcact ttttcgaatt ccattcttat ttattggtgc cttcatttta gctatgatca
     3721 cgatgccgca attatggtgg atcatcgtag cgcttatcgt tacggtattt ctgattacgg
     3781 cactttcatt tacaaggatg ggcaagcact tcatgatcat tcaaaaactg attgatcgtg
     3841 tgaatagcat tgcaaaagaa aacttactag ggattcgtgt agtgaaatct tttgtacaag
     3901 aagaaaacca attgaagtca ttcagcaaag taagtgagga cttaacaaaa cacaacatca
     3961 tagtcggtac attattttct gtgatgatcc cgtccttcat gctggctgcc aatttggctg
     4021 tggttggggc gatctttttt gtgagcgatc ttgttaaaga tgatcctacg ttaattggtg
     4081 gaattgcttc attcatgaac tacttgatgc agattatgat ggcaatcatt attggcggaa
     4141 tgatgatgat gatgacttca agagcagccg tatcgctgaa gcggattgcg gaaattttag
     4201 atgctgaacc agatcttact tatttagacg ttccagaaca agagttgacg ggttctgtca
     4261 cttttgacca tgtttctttt cgttatcctg gtgaggatac agatacgctt aaagatatca
     4321 gcttttctat caaacctgga gaaatggttg gtatcgtcgg ggcgactggt gccgggaagt
     4381 caacactagc acaattgatc cctcgtctat ttgatccgac agaaggcaaa gttgaagtag
     4441 gtggtgtaga tcttcgtcaa gtaaatgaaa agagtttaag acaaacggtt tcctttgttt
     4501 tgcaaaaagc aattttattc tcaggaacga ttgctcaaaa cttgcgccaa gggaaaaaga
     4561 atgcaacaga aaaagagatg gaacatgctt cttctattgc tcaagcaaaa gaattcatcg
     4621 aaaagttatc tgatcgttac gaagcaccaa tagaagaacg tagcagtaac ttttctggtg
     4681 gtcaaaagca acgtttatcc attactcgtg gcgttatcgg taatccaaag atcttgatcc
     4741 tagatgacag tacgagtgca ctggatgctc gctctgaaaa gctggtccga gaagcactcg
     4801 atcgagattt gaagggaaca acgacgattg tcatcgcaca aaaaattgct tctgttgtca
     4861 aagcagaccg catcttagta ttggatgaag gcagattagt cggcgaagga acccatgagg
     4921 aattggtagc tggaaaccgt gtctatcagg aaatctttga aactcaaaaa ggaacggagg
     4981 agtaagtatg actgatctaa taaaagcaag taaatttttc tatcactatt tgaagcggta
     5041 caaactctcc tttttcttta tctttatcac agtgatcatt gctacctatc tacaagtaaa
     5101 ggcacctcaa tacatcgggg aagcttttca agaattagct aattacgtag gaggcttgat
     5161 gcaaggcgta gacgataaaa gcaaatttgt ttctgtaata tggaaactct tgctgtttta
     5221 tgtactagct agtgcagcca actttatcta tagtattttg ttcacacaag tagtagggaa
     5281 gtcaacgaac cgaatgcgta tcggtctatt caacaaattg gaaaaattga cgatccgttt
     5341 ctttgattct catcaagacg gtgaaatttt aagtcgtttt accagtgact tagataatat
     5401 tcaaaatagt ttgaaccaag ctttgcttca agtcatcacg aatgctgttt tacttgtcgg
     5461 gatcttgatc atgatgttcc gccaaaatgt agagttagct tgggctacaa ttgcttccac
     5521 tccagtagcc gttttgatag ctgtcttgat tatccgcaaa gcacggaaat atgtggatct
     5581 tcagcaagat gaagtgggga aactaaatgg ttatatggat gaaaaaatca gcggacaacg
     5641 tgtcatcatc acaaatggct tacaagaaga aacgattgaa ggattcttag agcataatca
     5701 aagtgttcgc gaagcaacct tcaaaggaca agcttattct ggtctactat ttccgatgat
     5761 gcaaggaatg tcactggtca atacagcgat agttatcttt ttcggcggct ggttagcatt
     5821 aaatggtgat ttggaacgtt cggtggcatt aggcttagtt gtgacattcg tccagtattc
     5881 acaacaatat taccaaccac tgatgcagat ttcatctgga tacagcatga tccaattagc
     5941 gattacaggg gcaagacgat tgaatgaaat atttgatgaa aaagatgaag tcaaaccaga
     6001 aaacggtaaa gcatttgatg gtgttgaaaa aggtcttgcg ttgcagcacg tagattttgg
     6061 ttattctcca gaaaaactag tcttgaaaga tgtctcgatc aatgtggaca aaggagaaat
     6121 ggttgctctt gttggtccaa caggatcggg gaaaacgacg atcatgaatc tgctaaaccg
     6181 tttttacgat gtcaatgatg gttcagtagt catcgatgga atagatatcc gagattatga
     6241 tcttgatgca ttacgtagac atgtaggaat cgttttgcaa gaatctgtat tgttctccgg
     6301 aacgatcaaa gacaatatca cattcggcaa accggacgct tcagatgaag aagtcatcaa
     6361 tgcagcaaaa caagcgaata ttcatgactt catcatgacg ttagaaaaag gttatgatac
     6421 agagatatcc gaagaaaata acttattcag tacaggtcaa aagcagttga tcagtattgc
     6481 tcgaacgatc atcacaaatc catctttatt gattttggat gaagcaacaa gtaatgtaga
     6541 cacggtaaca gaagcaagga tccaaaaagc aatggatgaa gcaatcaaag gaagaaccag
     6601 ctttgtgatt gctcaccggc tgaaaacaat cttgaatgca gatagaatcg ttgtcttgcg
     6661 tgatggggaa gtcattgaag aaggcagtca tcatgagtta ttagcagaaa atggatttta
     6721 tgcggaactt tatcataatc aatttgtatt tgaaagttaa attaaaagaa cctcgttata
     6781 acggggttct ttttatatat tttatgttgt ttgtttttat aaggggca
//
LOCUS       DANKEF010000102         6688 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000102 DANKEF010000000
VERSION     DANKEF010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.103"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..115)
                     /locus_tag="PWZ48_002690"
     CDS             complement(<1..115)
                     /locus_tag="PWZ48_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2262303.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDR"
     gene            246..698
                     /locus_tag="PWZ48_002691"
     CDS             246..698
                     /locus_tag="PWZ48_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262304.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            712..1401
                     /locus_tag="PWZ48_002692"
     CDS             712..1401
                     /locus_tag="PWZ48_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262305.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1526..3877
                     /locus_tag="PWZ48_002693"
     CDS             1526..3877
                     /locus_tag="PWZ48_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HDL2262306.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3957..4352
                     /locus_tag="PWZ48_002694"
     CDS             3957..4352
                     /locus_tag="PWZ48_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262307.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4677..5294
                     /locus_tag="PWZ48_002695"
     CDS             4677..5294
                     /locus_tag="PWZ48_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262308.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5335..5634
                     /locus_tag="PWZ48_002696"
     CDS             5335..5634
                     /locus_tag="PWZ48_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HDL2262309.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5668..5835
                     /locus_tag="PWZ48_002697"
     CDS             5668..5835
                     /locus_tag="PWZ48_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2262310.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5880..6485)
                     /locus_tag="PWZ48_002698"
     CDS             complement(5880..6485)
                     /locus_tag="PWZ48_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262311.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2304 a          935 c         1303 g         2146 t
ORIGIN      
        1 cacgatcata taagatttct tgaacttcac gatagctaag gttataacga agatagtagc
       61 ccacggctac aataatcaca tcctgctgaa attgctttcc tttaaaatga ttcatcgtta
      121 ttcctcctgc tatctttttc tattattcta tcttatttga tagtagattt aaaactttgc
      181 aacagaacca attatcttat cgtagatata tattataaat ttaataagaa agcgaggtga
      241 gaatcgtgaa aaaaacgata ctaagtattt tttcagtatt tacaatatta attgggggct
      301 attttctttt gaatttagat tctgttaatt caaacgctca aataaaagat caaagagtag
      361 ctgaacataa taataaaaaa atgagtttga ccaaaaatat ctattctatg tcagatgaag
      421 aattgtataa agaatttggt gatttatccg gggaacagtt agaagaaagt attccaggaa
      481 ttagtgactc agataaaatt gttgtcttgt ctgctggtgg gcaattaatg ggagatggca
      541 aatccgaatt agttagttat aaacaaaatc acagacagtt tattgatgtt aatactgatt
      601 ccaacgctat ttcgattaag gatctaaaag agcttttaaa tagtaaaaga aatgaattaa
      661 agcagataaa agaaactaat tcagtggaat tagagtaaag gaagattcat tatgaaaaag
      721 atttttggtg tttctgttgg gctacttatg atgttaacat ttggaaatgc agtatatgct
      781 gctgagaaag aagttatttc agatccagag cagtacgcct cacgatactt tgctgatttt
      841 gaagaagaag ataaagtact tgcattgcca gaaggtggtt ttttaaaagg ggaagctaaa
      901 attgtatcta tggataatcc agatataatt caaggttcat ataattcaga aactgatcct
      961 gcttcagttt cagtgacaga agcaaaaaaa attgttgctg aaaaattcaa aaatgatatt
     1021 gaaactactc caattatgcc tcgaggagca tttccacccg atgttggtac tagcggacac
     1081 cttcttgtac tagcaggagg aggaagctat aaatcacagc catttagtgg tgcgggatgg
     1141 cgttttggag gtatgggtgt aaaaccagct gatggtacag gcaacttttt acgttggaaa
     1201 tcctacattg acgatggaag agtaggaaat gtaaatcaag ccaacgcaac taaatctggt
     1261 tctattcaag gaatggcatt gtatgctcgt gaaggattca aatactttgc accaaacggg
     1321 cctggttgga caatgaatat aggtatttat tacacttata atcctgttgc aggtacgtat
     1381 tatgttgttc aaaatattta ataaaacagc acaaagagat ttctttgtgc tgttttatta
     1441 tttttagttg ttgacaaaca ctaaaatata aaatataatc aacacataaa cgaacgtatg
     1501 ttcggcaaga aagcagggga tttttatgga tgagatggtt ttaagtactc agaaatggct
     1561 aaacaagaaa tacagtaatg ttacagggtt tgataaggtt ccagaaaatg ggcgaacagg
     1621 gtggcctaca atctatggac ttattgaagg actacaagta gaacttggta ttactaatct
     1681 agtagctaat tttggaccta ccactgaaaa aatgtacgat aatcaggtta ctcctaaatg
     1741 gggcaaaaat ctacctaaaa atattgtatt tcttattcaa ggtgcctttt ggtgtaaggg
     1801 gattaatcca ggtggcttcg atggggtata tactccatat ttagatactg ctgtaaaaga
     1861 gttgcagact gatgcagggt ttaatggaag cgctgtaact ggagttttgg atgcgaaatg
     1921 ggccaaagcg ttatttgata tgagtgcttt tgttcttgtt cccggcggag atagtaaaat
     1981 tagaaaaatg cagcaatggt taaatgtaaa ttatttagaa tacacgggaa taatgccttg
     2041 tgatggtatt tatcaaagaa attcaaatca agcattaatt tttgcattac aagctgaact
     2101 aggatattct cccagtgaag caacagggtc ttatggtaat gggacaacta gtaaaactta
     2161 tcctgtgtct gaaggtaact cgggaaacta tgtaagaata attcaatatg gattatatgt
     2221 taatggttat tttgaagacg gtacattcga tggtattttc actaaatata tgggtttaga
     2281 agtactggca ttccgaaaat ttatggtatt gcctgaatat actgaaatag cagatgttgt
     2341 tgtaataaaa ggactattgt ctagcgctgg agatattaga agatctgcag atggcgcaga
     2401 tacttctacg caattaacta gatctcaaat ccaaacattg gttgataatg atataaaaat
     2461 agttggtaga tatcttactg gtactgttgg aattggaaaa gatgaacgaa ataagtatct
     2521 aactacagag gaattgaata atattttttc taaaaattta tctgtgtttc ctatctatca
     2581 agatggaggt gcagcattag cttattttac ttatgatcgt ggcttatcag atggtaagaa
     2641 ggctatagat gcagcaaaaa atctaaatat accattaaca acagttattt attttgctgt
     2701 tgatctagac atgttagggg aagaaattct tgcatatgct ggagaatatt ttaagggggt
     2761 atctgctgta atgtcatata gcgggtatca aacgggtgtt tacggaacaa gaaatgtttg
     2821 ttctcaaata atcaataacg ggtatgcttc gtttgcattt gtttcagata tgtcgactgg
     2881 ttatagtggg aatttaggtt ttcctatgcc tagacaatgg tcttttgatc aatttgttga
     2941 atttaccata ggaagtggca acggagctgt tggtatcgat ttaatcgcta catcgggtag
     3001 agatagcgga tttaatgagt tatcaaatga tactaataat gataattaca ttgccaaata
     3061 caatcagaag gttataaatc agatggtacg tgcgtatcaa tatctatcac aagctggtct
     3121 tgataatcct tggaaccctc atttgacatt ttatagatat gtaaattatt ctggtttgtc
     3181 atgggacatc atttcttctc ctgtaacaga gcatgataga aaaatttatg atgaatatag
     3241 gaataaatta actaatagcg agggcctgta taattatttt atagatccta atactggttc
     3301 aataataggt ttgcctcact taattgtgac gttacaatca cagatgtttc tagccgacac
     3361 aatcaatgat agtgtttctg actttgcagg ttggttagga gatttaatga cttgctgggg
     3421 tgaagttaag aaattaggaa tacaaatgga gcaaggtgta tttgcattag taggtacggc
     3481 tgcaggtgga acatttagtc tagaagattt ataccaagat gttgacgcgg taaatgttta
     3541 taccttagca aaaagaaatt ctgattcaac agctttatcg gctttaaaaa cgtactattg
     3601 tgataagggt gctattgata gatttaatca atttgttaag aacagatttg gttcttcaga
     3661 taaaatcgct tcagtaacaa atagtttatt agaaaatgac gaaacagttg atatcttagg
     3721 tgtagggata atttcatttt tcattgataa aatagttaat aattcacttg ggacagattt
     3781 tacttatctt gattttattg gagatgaggg gaaagttgca aatggatttt ctgaaaaaat
     3841 tattcaattt caagaaaata gaagtaaact taattaagaa tcgtttacta tttaaacagt
     3901 gtccttttcc tttttaaata tatacgataa aataagtata tgaattgtaa aaggacatga
     3961 tgattatgga agaaatagaa tggaataatc tgactcaaaa taataaaaat aattctggat
     4021 gtggaattat ctttatgttt gttatagttt tgggaatagt agtactttta ataactgtca
     4081 agatagtaga ttactttgcg aatactcctg aagaacaaat acagagaaaa cttcctgagt
     4141 ttgaaagagt accaccttat gatccaagag ataattcatt tcaagtaaaa aaattaaaag
     4201 agtctccaaa tgaaatgtgg tttgaagtta cagttgaatc aagcgacttc aaagaaaaaa
     4261 agaaagaata caattatatt gtaaaggcaa aagtatcaaa aagatttatt tttaacccta
     4321 aattagaaat atatagtgtt gtaatagaat aatagtgtta aaaaaattgt aaaagttaaa
     4381 gaagtcaaat tctgatgtga aaaataaaat ataccatgga agacataaaa gaaaaactgg
     4441 ataactagtt tatttaatgg cagaccgtta aggcctgcca tttttatgta ttttagcgga
     4501 ataaatataa tcatgcggga gtgtaaactt actgtttcaa aaagtatact ttttgacatg
     4561 ccttgatttt ctttgtttat gtatggcata ataaccacat aatacaattt gaaacgtttc
     4621 tgatttaatc tgattgtttt gaaacaattt tagtgaaaaa agggtagata aaaacgatga
     4681 gaaaattttt ttatatccgg gtcagtagtt atgatcaatc gatcgaacga caactgattt
     4741 ccgcgaagga attaggaatc cctgaagaat atatatttgt agaaaaagcc agcggcaaag
     4801 attttaaacg ccctgaatat caattaatga aacggatgtt tcgaaaagga gatttactct
     4861 atctacaatc attagataga ctaggcagaa ataaacaaat gattttagaa gaatggcgag
     4921 aattaactaa ggaaaagcag attgatattt ctgtgttaga catgcctctg ttagatactg
     4981 ctagttacaa gcaactagat gggttagaga cgcttatctc tgacttaatt ctgcaattac
     5041 tcagctatat ggccgaagac gagcggaaac gaattaatag ccggcaaaga gaaggcgtag
     5101 aagctgcgaa acaaaagggt gtaaaattcg gcagaaagaa aattgcttta cctcctgaat
     5161 ttcctgcgat ctattctgac tggaaagcag gaaagataac tgcagtctat gctatggatc
     5221 aattgggcat gaaacgaaat accttttatc gacgggtgaa agaatatgaa cataccttgc
     5281 accacttttc ttgatagata taacagctat tagacgggaa gggagagttt gaaaatggac
     5341 ttaacgaatc aaagggtcgt ttttactgga acgctgcagc aaatgacacg aacccaagcc
     5401 atcggtttgg ttcactcttt aaacggccat tgtcaatcaa gtgttacttc aaaaacaaat
     5461 ttcctggttt gtggtcagta ttctaagtcg ttgtttgatg attctctcta tacaaaaaag
     5521 gaacaaaccg cgcgggactt gattgcgtta ggtcatgaga ttaccatttt atcagaagtc
     5581 gaattttatg cgttaattag tcaacagttg aaagaatggc aacggtattt ataatcgtta
     5641 gtaaaattta caaggaggaa aatttttatg aagcgaaaat cttatgatcg ttggggcatt
     5701 gaaataacta gttttaatcg aaatattgct tttcacgtgg agtgtgactg tggggaatta
     5761 gcaaaaagtg tttatcaaaa aaattacttt tactgtccta aatgccatcg ccgatacggg
     5821 gtacatggaa catagaaacg aaatagtgcg gtagaaaagc gaatgaatgt agtatatttc
     5881 tacaaatcat tcgctttctt tattttataa atagttggtc gagagatccc gttttctttt
     5941 gcaatctctg caactggaat gccttgcttt aacatgtcca ccacggtttt aaaaactaag
     6001 cgtttttgcg gatctgttga gttaatagaa tactcgattg gtcgtccctt atatcggccg
     6061 gctttttgag ccaataatac tccttgccgc tgacgttcac gaattttctc acgctcgttt
     6121 tgtgcaatat aacttagcag tgataaaaac atatcgaaca tagcggtatc caggagttca
     6181 tttccagtat taaaatctaa aaactgtgca tctaatattt ttaacgctac ttttttctgt
     6241 ttcataaaag caactgtatt ttttatatct tcataatctc ttcctaaacg atctagtgat
     6301 gtaacgatga cgcaatcacc ttcacggata taatttagta atttttgaaa ctcaggacgc
     6361 tcagtatctt taccactggc tttttcttgg aataatttat caatgttaag tccttccatc
     6421 attgccaatt gtcgatccaa attttgatct tgtgaagata ctcgaatata gccaattttt
     6481 cccataaaaa actccttttt aaaagtgata taatttacat gtaaaattta cttgtagttt
     6541 tactgctatc atagcatttt taaaaataaa ttttatgtag acttaaaatt tacttcatgg
     6601 agggtttaaa tggatgaaat caaaagtaac aaaaaagcat ttggcattta tttaggagaa
     6661 cctagtacag aacaattaaa ccaatttt
//
LOCUS       DANKEF010000103         6306 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000103 DANKEF010000000
VERSION     DANKEF010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.105"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            42..197
                     /locus_tag="PWZ48_002699"
     CDS             42..197
                     /locus_tag="PWZ48_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002392227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262312.1"
                     /translation="MVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLLNL
                     FNRIVGA"
     gene            210..710
                     /locus_tag="PWZ48_002700"
     CDS             210..710
                     /locus_tag="PWZ48_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HDL2262313.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            807..2654
                     /locus_tag="PWZ48_002701"
     CDS             807..2654
                     /locus_tag="PWZ48_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HDL2262314.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2657..3553
                     /locus_tag="PWZ48_002702"
     CDS             2657..3553
                     /locus_tag="PWZ48_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2262315.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3603..3992
                     /locus_tag="PWZ48_002703"
     CDS             3603..3992
                     /locus_tag="PWZ48_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HDL2262316.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3979..>6306
                     /locus_tag="PWZ48_002704"
     CDS             3979..>6306
                     /locus_tag="PWZ48_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDL2262317.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2124 a          936 c         1350 g         1896 t
ORIGIN      
        1 aatggttttt aaccaatgct caatctttag tcttactagc gattgtggtc attggtttgt
       61 ttttaggatt taaacgcgag ttttccaaac tgattggctt cttggtgatt gctttgattg
      121 cggtaggtct ggtctttaat gcttctggtg tgaaggatgt cttactcaat ttgtttaacc
      181 gaattgtcgg tgcctgaggg ggaatcacaa tggaacagat acgtgtttat attgcgaacc
      241 ttggaaaata caatgaaggt gagttggtgg gggcatggtt taccccaccc gtcgattttg
      301 atgaagtgaa agaacggatt ggattaaatg acgaatatga agagtacgca attcatgatt
      361 atgaactgcc ttttgagatt gatgagtata caccaattga ggaaattaat cggttgtgtg
      421 gtttagcgga agagcttaaa ggaacgccaa ttggtgaggt cgcttcagag attcaacatg
      481 ccttcttcaa ttctttcgag gaaatggtag agcatgtgga tgatatcatc tattatcccg
      541 attgtaatga catgagtgat gtagcgtatt acttgattgt agaagctggt gatttaggcg
      601 aagtaccaga acacttgaag acgtatattg attttgatgc gtgtggtcga gacttggaga
      661 ttggcgggaa ttatttggtt acgagtcgag gggtttttga actaatgtaa atcatttgtt
      721 agaattgacc agaaatttct atcgtacttt tacttttcta tttgatatac tgaaatagaa
      781 aatatttgtt gataggggtg ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc
      841 atattcattc aactgcgagt cagtgctttc aggataaaaa ggttactgct aaggaatggg
      901 tagaagaatg tttaaacaaa ggactcgatt gtgttgcatt aactgatcac aatacagggg
      961 aaaatattga tgaatataag aaagtggcta tcgaaaatgg cttagatttt tttcctggag
     1021 tggaattaac gtgtggagaa aatggaactc atttactagt cctatttgat aaaagtgcat
     1081 gtacaaatga tatctctgat ttcttaacca agatgggtat aaaaagagag gattgggcca
     1141 gcgctgcagc ttgctcaatg aaatcagttt tagaagttat tgattttgct gagcacgagg
     1201 gcttgctcgt tattcctgca catatagatg agtttaatgg aatttcttca gcaaaatttg
     1261 gaaatctagg aaagatattt gaatcagaaa atatatatgc agtacagacg gttcaaaaag
     1321 aattttttga aaatcgctca caaatgattc cctcatctaa gcgtgacact atctatgata
     1381 gtgtgaatgc cagatatgat ggtagagttg gaaaagatac tttggaatct tggtataaat
     1441 cagttgttga ggcagaaaaa aataatatga catttcttag cttttctgat aacccacact
     1501 cgaaaggaaa ttcaaaacat ggactttggg gaataggcac aagatattcc tatataaaaa
     1561 tgaaggacga acctgacttg ggatcacttc gcgatgcttt aatgctaggc gaaactcgag
     1621 ttcattctga tttttctaat ttctccatta atgaaaatga agttttattg gaaaaattaa
     1681 gcttttctgg gaccactctt agcacaaaag aagttgtagt cgaattcagc gataatttaa
     1741 cttcaattat tggaggaaga ggaacaggca aatcttgtat tacaagattc ttggtctatg
     1801 tccttggaaa ggaagcagaa cttgatcaat tttctgaaat acaaagtgat tatcagaact
     1861 ttgcacaaat tgaacataat ggctctggaa tatttttgaa ggatacaatt gtgaaactga
     1921 atattttcta taaggggaca aaatatcaaa taataagaac acaggataga cattctattt
     1981 atgaatttac acaagagaat ggtctggttg aaaaagaaac agaacggttg agaatgattt
     2041 ccgataaggt gagtatatac tctcaaaaac aaatttatga aatttcaaaa aatcaaacta
     2101 gtatactaga attactagat ggatataact ctgatttgat ttcagagtat aaaaatgaga
     2161 ttgaaacatg tgtaaacgaa ataagaaaat taaattggga tattgtttct gtgaaaaaag
     2221 aaatacaaga taaggcgaag gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa
     2281 agctatctca taagagttat aaagatgttt atgatgagta ttcaaaagag acagatattt
     2341 atagggaact gaaacgtgat gtggaggcac ttaaggaaat tcccaaaaat ctaactgata
     2401 gtgttgacaa aattgacttt ggcaatggta taaaagttac agacgaaatt gatgaacacc
     2461 gcggagaatt gattaagaat ttagtgacca atcgggctaa gattcaaaca ataattaatg
     2521 aaatgtcaga tgaaattgat agttatagag cagagctgaa taaatcagat tggaaagtta
     2581 actataatga ggtttcaaag aggtatcaaa gaggtatcaa aaacttagtg aagagttatc
     2641 tcaagatgaa ttaaaaatgt tacaaaacct tgattcttta tatcaatctt tatcaaaaac
     2701 tgaagagcga ttggaagaaa ttattcaaca agaaataaag cttaaagatt accaagttga
     2761 ggttgactca gcaatgtcaa gaatgaataa atcatatcaa aatctctatt ctgagagaag
     2821 tagttcctcg cgcgaaattt tttctgatat tacaggaata gaggctacta ttcaaccaaa
     2881 tagggatttc aataattatg ttaaagagct cagagcttgt attaataagg aggacacttt
     2941 tgacagtgag tttggatctc ttattgataa gctgacaaaa aattccatta ctaatcaaga
     3001 tatatataat gacatacttg atataagagt tagtggggaa agtagtattt tttcagataa
     3061 aaaattaatc aattctataa gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct
     3121 taagccaaat gatttagtag ttatccgact tgaactaaat ggaaaaaaag ttagtttatc
     3181 gaatgctagt gctggtcaaa agacatctgc aattctttct atgattttgg cgtatggaaa
     3241 ttcaccactc atattggatc agcctgaaga tgatttggat agtcaattaa ttaataactt
     3301 aatcgtaaga agtatcatta caaaaaagga aaacagacaa attttaattg taactcataa
     3361 tgcaaatatc ccagttaatg gtgattcaga atggttgatt agcatgggag attcaaaaga
     3421 aatatcggct gatcagtgcg ggtcggtaga tgaacccaat atcaagccaa gaatatgtac
     3481 tgtgatggaa ggtggcgagg atgcttttac aaatagagcg aaacgctatg gttttaaaca
     3541 aattgtagaa taacactaga gggtgagaca tattattatg tccgcccttt ttaggaggaa
     3601 aaatgaaaaa aataaaaagc tataccagta tctgggcagt tgaaaaagtc atttacgcaa
     3661 tcaatgattt tcagttgcct tttcctgtga cttttaacca aatggcttgg ttcgttttat
     3721 ctttactgtt tgtcatcgta tttgcccatg tgcctccact atctatgata gaggaggtct
     3781 ttttaaaata cttaggcata ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg
     3841 atggcaagaa accactggga tttttacgat cctttatcag ctatcacttg cgatttaagg
     3901 tgacttttgc aggaaagaaa gtcaaagaac agaagaaacg atgggatgaa cccagtactc
     3961 ttgtaaggag tgtgaactat gtatccgatt aagtatattg aaaataatct cgtctttaac
     4021 caagaaggcg agtgttttgc ctactatgaa ttagtgcctt acaattactc ctttttgtca
     4081 cccgaacaga aatttcaagt gcatgataac tttcgccaac taattgcgca aaatcgtgaa
     4141 ggcaagatcc atgcgttaca aattgccaca gaaagtagta ttcgcgccac acaagaacga
     4201 tcaaaaaaag aaattaccgg tagactcaaa gaagtagcga aacaacgaat tgatatacag
     4261 acagatgcat tagtatccat gattggcgat agtcagattg attatcgctt ttttattggc
     4321 tttaaactaa ttgcgacaga tgaagaagtc aatctaaaga gtctcaagaa atctttcttt
     4381 tctgggcttc aagaatttgt ctatggagtg aatcatcatt tgatgggcga ttttgtttcc
     4441 ttatccaatg aagaaattcg ctgctattct aagcttgaaa aattaatgga aagcaaattg
     4501 gctcgaagat ttaaagtcag gcgagtcaca ccgagtgacc tgacgtattt gattgaacat
     4561 atttatggcg aaaaagggat accgtttgaa gaatacgaat ttcagctacc aaagaaaaag
     4621 ctaaaatcag aaacactggt gaaacgttat gacttattac gtccaaatcg ttgtttaatt
     4681 gaagaaaaac caaggtgctt acggatggaa catgagaatc atgagtcgta cgtagcgtat
     4741 ttaacaatta atacaattgt gggggagatg gaatttcctt cctcagaact tttctattac
     4801 cagcaacaac aatttacatt cccaatcgac acaagtatga atgtagagat tgtgacgaat
     4861 aaaaaggctt tagctaccgt tcgtaataag aaaaaagaac tgaaagattt agacaatcat
     4921 gcttaccaat ctgacaatga aacgaattcc aatgtcttag atgctttgga ttccgtggat
     4981 gaattggaaa ccaccttgga tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt
     5041 cgagtgagtg ccgaatcagt ggatgaacta aagcgacgct gtgatgaagt gctcgatttt
     5101 tatgatgata ccaatgtaaa actagtccgg ccctttggcg atatgatggg gctacatgaa
     5161 gaattcttac cctcaagtaa acgatatatg aacgactata ttcagtatgt tacgtctgat
     5221 tttttggcgg ggcttggttt tggggctact cagatgctag gagaattaga agggatttac
     5281 tttggctaca atgtggatac aggtcgtaat gtttacctga aaccggcatt ggcttcacaa
     5341 ggagtcaagg gttcagtaac caatgcgctg gccgccgctt tcctgggttc tcttggtgga
     5401 ggtaaatcct ttagtaacaa tctcttagtc tactatgctg ttttgttcgg tggtcaagct
     5461 gtgattgtcg atccaaaagg ggagcgagga gggtggaaag aaaccttgcc agaaattgcg
     5521 gatgaaatca gtatcctcaa cttgacgagt aagtcagaaa atcaaggtct gcttgatcct
     5581 tatgtgatta tgaagaaaaa gaaagactcg gaaagtttag caattgatat tctgactttt
     5641 ttaacaggaa tttcaagtcg agacggggag aagtttcctg tgttacgtaa agccattcga
     5701 acggtgactc aaagtgatca acgggggtta ttacaagtta ttaacgaatt gcgaaaagac
     5761 gggtcaccaa tcgcggaaaa tatcgccgat catatcgagt cctttaccga ttatgatttt
     5821 gctcatttgc ttttctcaga cggctcgatt acccaatcga ttagtttaga taaacagcta
     5881 aatatcattc aagtggcgga tttggtttta ccggatgcag aaacgacttt tgaagaatat
     5941 accaccatgg aattgcttag tgtttcgatg ctaatcgtca tttccacttt tgccttggac
     6001 tttatccata gtaaccgaga aatcttcaaa attgtcgatt tggatgaggc ttggagtttt
     6061 cttcaagtgg ctcaaggaaa aacgttatcg aataagcttg ttcgtgctgg tcgggccatg
     6121 aatgctgggg tctatttcgt cactcaaaat agcgacgatt tattagatga acgtttaaaa
     6181 aataatatcg ggttgaagtt tgcttttcgc agtaccgata ttcatgaaat caagaaaacc
     6241 ttggaattct ttggtttgga tcaggaggat caaagcaatc aaaaacgcct gcgagatttg
     6301 gaaaac
//
LOCUS       DANKEF010000104         6174 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000104 DANKEF010000000
VERSION     DANKEF010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.106"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            725..811
                     /locus_tag="PWZ48_002705"
     CDS             725..811
                     /locus_tag="PWZ48_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2262318.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="PWZ48_002706"
     CDS             complement(932..1621)
                     /locus_tag="PWZ48_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262319.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="PWZ48_002707"
     CDS             complement(1628..1906)
                     /locus_tag="PWZ48_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262320.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="PWZ48_002708"
     CDS             complement(1866..2780)
                     /locus_tag="PWZ48_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2262321.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="PWZ48_002709"
     CDS             complement(2762..3142)
                     /locus_tag="PWZ48_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2262322.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3593..3880)
                     /locus_tag="PWZ48_002710"
     CDS             complement(3593..3880)
                     /locus_tag="PWZ48_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2262323.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3900..4124)
                     /locus_tag="PWZ48_002711"
     CDS             complement(3900..4124)
                     /locus_tag="PWZ48_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HDL2262324.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4579..4749)
                     /locus_tag="PWZ48_002712"
     CDS             complement(4579..4749)
                     /locus_tag="PWZ48_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262325.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4764..5297)
                     /locus_tag="PWZ48_002713"
     CDS             complement(4764..5297)
                     /locus_tag="PWZ48_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2262326.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5318..6160)
                     /locus_tag="PWZ48_002714"
     CDS             complement(5318..6160)
                     /locus_tag="PWZ48_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262327.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1635 a         1151 c         1040 g         2348 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccaac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag cttactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc ccccactctc cacaatacaa
     3361 aaaagactag caaatgctag ccactttttg taaagtttcg cttgtttcac cttcggtgaa
     3421 attgttgcaa atcaataatt aggtagagct acattttttt cgttgttcaa agattcttat
     3481 gccatttact attaataaat ctataaatat gataattttt cggctctata atttatggga
     3541 ctgatgaatc agaattgatt cttcagtccc attttcattt tattccaaag aattaactcc
     3601 aaataccaat agaagcccat cctctatata cttgagattc cttactaaca atttcttgta
     3661 attctattaa agatttaggt aatccaaatt tatgttttaa aatatatctt ccaagaaaac
     3721 tacttaattt catactaact tcaccaacac tttttccctc actgatttct ttagaataaa
     3781 gaaagagctt ttcttttaag tcaggatagt catctatagt tgcttcattg tatactttac
     3841 taattgcatt taaaagtttt tcttctttag taaaatccat tataataacc tccctaccac
     3901 taccttctag gtgcccatgg accatgatta acccaaccat taacaataat ttttccaatt
     3961 tctcctttag cttgattcca atctacccaa catttttctt tgttacaata aagaccattt
     4021 ccatagtaag tagctgcatc aacttttatt cctgtaccga tgccagctag acaagttcct
     4081 aatattccta gaataacaca atgttttaat actttctttt tcatgattgt ttttcctttt
     4141 cgttttttta aaattgcttg ttatattaaa taatcttagt aattttatta tataacattt
     4201 tattatataa caatgattaa cgtcataaaa agtagttttg aattcataat cgtttaaata
     4261 aaattataag atcagttatt taaacagcta gtagacataa tgtatatact accaagtaac
     4321 gcctgtacca agggatccag gcgctgattt tgtgaatttt tattggtcta ggttaaaagc
     4381 tctttttaag gcttggtttt ctgcggttgt ttctttctaa ttctttgtga atattgatcg
     4441 gtcatttttt aaatacttgt tatttttgtt tgtgtttgtt atgcttttta catatctcat
     4501 attcatttat tattcttttc taaggttaat aataggataa tctctttcag aaagactgct
     4561 cgtacgagca gtcttttctt atttatcaag tttttctaca aatacttcct cattttgact
     4621 gttgtagtaa ttaggattga gttttagttc ccctttttta tttcctttgt tggccactga
     4681 taataacatt gggctaaagc ttaatattcc tagtaggata gaaccgaata gcaagtgttt
     4741 ttttgtcata agtatttcct tcttcatttc catagctgcc accatttttt ccaattcttt
     4801 gtttgttcgt ccattgtttc tgttgcttca gaaagagtcg ttgaactatt ctctgaagac
     4861 tcttccgtat aagtcaactg cagttgctcc tgaagttttt cgatttgttg gttggcttgc
     4921 agggtgagtt gttgttgctg gtcgagtagt ttttgtatat gagaattatt ttgctctttg
     4981 agggcaagtt gttctcgtaa aaattcattt tcttcacgca actgatctag caacttgttt
     5041 gcaaccacgt ttttgtttgg tatataagca tttttatcgt tgttttgttc atgtttatgc
     5101 aacttatcta gcaacctttg attgtctaag ttgcgagcaa gttgcaatac tccagaacgc
     5161 aatacaacct tataaccacg ctcattttta gctatcatgg aagaatctaa tttttggata
     5221 tgtttcgata ttgcttgcct actcatctta aaaagatctg acaattcctt tattgtaaag
     5281 gtttcttgtg gttgcatact ggttgcctcc taggttgcta ctggaataaa ttatctaaat
     5341 cagataataa actttttttc tctttttccg aaagtgcgga ctctgtttgt ttttcaagag
     5401 cttttttctc tttttctagt tgtttgattt gttggaggag ttcaagttct cgtttcttct
     5461 gtttgatttt ttcgttaatt gttgtagcgg gttcttcgta ttctttttgt ttatcagcta
     5521 aagccaatgc tttattttgg tcttctttgg ttggtgcatg tttgttaaag tcgtctgctt
     5581 ttttgatctc aggtttgaac gtaaattgat agccaataac aggttttccc cgtccttttc
     5641 cgtatttctt tttgattgtt agtcctttga agattgcagt tagttcttga cgtataggtt
     5701 ttataactcg tttatctata tcaggttgct tataactttt aggaatatct aataattcaa
     5761 gaaagtcttc tttggagaaa tatgcgaaac ctttggttct gtattgcttt aacagacgaa
     5821 acatagtctt tgcatagcca ctttgtagct cgttgaattg ttgtaaagaa tagcgtaccc
     5881 actcgtctaa attatttaac aaaggcagtg ctttttcgta aattcgtata tctacgtaag
     5941 gattttctac gtccccgttg attttaaatt ctgtaaacat tacaaacata tcgtaagaaa
     6001 gcccagactc acttctacga ccaaagcgta aagataagat tttttcataa gttgctttta
     6061 aatcttctac aaatcgtaca tttgcagttg ctttgtagtt acttaaatct tttagttggt
     6121 caaacgtaaa acgaactgtc ttatcccctt tttcacgcat tctagacacg atgg
//
LOCUS       DANKEF010000105         6071 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000105 DANKEF010000000
VERSION     DANKEF010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.107"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            371..985
                     /locus_tag="PWZ48_002715"
     CDS             371..985
                     /locus_tag="PWZ48_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262328.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(1150..1392)
                     /locus_tag="PWZ48_002716"
                     /pseudo
     CDS             complement(1150..1392)
                     /locus_tag="PWZ48_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455809.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1596..2915)
                     /locus_tag="PWZ48_002717"
     CDS             complement(1596..2915)
                     /locus_tag="PWZ48_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element IS1678 family transposase"
                     /protein_id="HDL2262329.1"
                     /translation="MTSLHKNQVKFNSNITISHTGGQLSSDSGLVLVKELMNIFGFSE
                     LAKQHIHIEDERAYFTHDNLSILEQFIMQLIAGYSADSAANLLRQDPVFKAVLDRKEL
                     ASQSSLSRFLDRLSEENIHELQALNQELIDKARLIRNDTELIIDLDSTHSDTFGHQEQ
                     TNYNTHYQTYGYHPLVAFDGLTGDFLKAELRSGNQYTSKGVKEFLTPLLEHYNHSLPN
                     TDILVRGDSGFATPGVYDSCESKKSHYVIRLKNNRRLGQIAEKSVLYGDNQKWEEREV
                     QYFSTTYQAQSWSQSRRVCIRSTREAGELLFRHEFIVTNLSENVSPDTIFSIYAKRGT
                     MENFIKEAKAGFYFDKTDSPRFLENHVRMMLSLIAYNLVNFLRTIGFEEVQKGMTIHS
                     IRLKFLKVAGKLVQTGRRVYLKLSSYHVYQNEFYRVFARLRRASQWI"
     gene            complement(3070..3657)
                     /locus_tag="PWZ48_002718"
                     /pseudo
     CDS             complement(3070..3657)
                     /locus_tag="PWZ48_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(4295..4552)
                     /locus_tag="PWZ48_002719"
     CDS             complement(4295..4552)
                     /locus_tag="PWZ48_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HDL2262330.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4545..4814)
                     /locus_tag="PWZ48_002720"
     CDS             complement(4545..4814)
                     /locus_tag="PWZ48_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HDL2262331.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
     gene            complement(4945..5124)
                     /locus_tag="PWZ48_002721"
     CDS             complement(4945..5124)
                     /locus_tag="PWZ48_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262332.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(5505..>6071)
                     /locus_tag="PWZ48_002722"
     CDS             complement(5505..>6071)
                     /locus_tag="PWZ48_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262333.1"
                     /translation="EEQLYLESETSQTKVTVSHNRLDVYKRRPETVKKGTELLRLETE
                     KYIMNVYLERSELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATID
                     DYYLVFTRFLTCGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTY
                     TYDDTEEIQNELDRKRMPYQRFERKKRQ"
BASE COUNT         1882 a         1116 c          952 g         2121 t
ORIGIN      
        1 gaagcatagg tatgctatca ccctgcttct tcttttgatt tgtatgatca atttattcta
       61 tcactttact aattaatttg aaaatctaat aaaaactctc actctaaaag agtgagagac
      121 tgcaaattag agtacggact tttttggagt tccgttcttt aatattaaca aatgaataaa
      181 atccttacaa catttgagaa taagttatca gtagacactt acaaaaaatt ctcatactat
      241 ttcctccatc attagttttt gtctatataa ggtttaccct aaatagacac tggtataaca
      301 ttgaaaaata tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta
      361 ggtaaaagtt ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg
      421 ccagattgaa gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg
      481 cgcaacgatt actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga
      541 tatttttatt gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc
      601 agtcaattac ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc
      661 tgaagcaatt ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt
      721 ggccatgatc gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa
      781 gatcgcaaaa gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa
      841 agatccccaa cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc
      901 gattgctaag attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa
      961 agacagcatg gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta
     1021 atttcctaca tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata
     1081 aaattccaac aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac
     1141 ttatttacgt taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc
     1201 cttattatct tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact
     1261 aattcctata gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat
     1321 ttgagattta tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata
     1381 cataaagcta tccctgaata attcatagct ttaattcttt ggcacatttt attgaaatat
     1441 gtatcactaa tatccctatc aactgatttt gactaaaaag ttacttcaaa cattcactgt
     1501 tcaaaaatat tgaggaataa attttgggtg taaggcattt tgagcatact tctccctgga
     1561 taacttccca gtaaaaaaat cgttagattg ttggattaga tccattgact ggctcgcctc
     1621 aggcgagcaa agaccctgta gaattcattc tgatacacat gataactaga taatttgaga
     1681 tagactcgtc tacccgtttg gactaatttc ccagcaactt tcagaaactt caatcgaata
     1741 gaatgaatgg tcattccctt ttggacttct tcaaagccaa tcgttcttaa gaagttgact
     1801 aagttgtaag ctatcaagct gagcatcatt cggacatgat tctccaaaaa gcgaggactg
     1861 tctgttttgt caaagtaaaa gccagctttc gcttctttaa tgaagttctc cattgtgcca
     1921 cgtttggcat agatagaaaa gatggtatca ggagaaacat tttctgatag attcgtcacg
     1981 ataaattcat gtcgaaagag tagttcgccc gcttcacgtg ttgagcgtat acacacgcga
     2041 cgactttgtg accaggattg tgcttgataa gtggtcgaga agtactgaac ttctcgttct
     2101 tcccactttt gattatcgcc ataaagtact gatttctcag ctatttgacc tagtctacga
     2161 ttattcttca gtcgaataac ataatgactc ttttttgatt cacacgaatc atacacacca
     2221 ggtgtagcga acccgctgtc tccacgaacc aagatgtcag tgtttggtag agagtgatta
     2281 tagtgctcta ataaaggtgt gagaaactcc tttacgcctt ttgatgtata ttgatttcct
     2341 gaacgtagtt cagcttttaa gaagtcacca gtcaatccgt caaaagctac caatggatgg
     2401 taaccatagg tttggtagtg tgtattataa ttcgtttgtt cttggtgacc aaatgtatct
     2461 gaatgggtcg aatctaaatc aatgattaat tccgtatcat tacggatgag gcgtgcttta
     2521 tcgataagtt cttggttcaa agcttgaagt tcatggatat tctcctcaga tagtcgatct
     2581 aaaaatcggg aaagtgaaga ttgagaagcg agttctttcc tatctaaaac agctttaaac
     2641 acaggatctt gtctcaggag attagctgca gaatcagctg agtaaccagc aattaattgc
     2701 ataatgaact gctcaagtat ggataagttg tcatgagtaa aatacgctcg ttcgtcttca
     2761 atgtgaatgt gttgcttagc cagttcagaa aaaccaaaga tgttcatcag ctctttcact
     2821 aggacgagac ccgaatcact cgataattga ccacctgtat gagaaatagt gatatttgaa
     2881 ttgaatttta cttggttttt gtgtaagcta gtcatgaaaa gaactccttt cttttggttg
     2941 gtttagtcac tttaaccata gcagaaaggg gttctttttt catcacttaa cgggtgaaga
     3001 tgaaaaagta attgtaagct gccgtgaggc agtttcacat ggttttaatt aaaagtatga
     3061 attattcagg ctatcattaa ttttataaaa aaaattaaat gtttttagca ctgccatata
     3121 acattcaaaa cattcttcta tagttggttt acttccttga ggagaatgaa ccaatttatt
     3181 acgtttttct atgaaatttt taatttcctt tcttatttct ttcggaaact caatattaaa
     3241 gtacccttta aacatgctcc ctaataattt gtctacaggg ggagattgta aattctcaaa
     3301 tattagagtt aaatcaggtt tctcttgttg ataaaattcc tttaccccta tttcacaggc
     3361 tgtaattatc gaaataaacc tatctctagt atttgatata tttttacttc ttttaaggag
     3421 tataagagaa aaggggatac tttcgtgtcc atgcatgtaa ttaactaatt gttgagcttc
     3481 agaagttata tctctaatta tatagttaac ggaattagga agtgtcttta actctaatga
     3541 aatttctatc caattactat cttctaatct gtatatgctc gaagtataat ttgattcgtc
     3601 agcaagcatc gctacatccc aaacactgat cataaaatct tttaaacgtt caatcatttc
     3661 agcaatattg ctaaaatcag ctaatttttc tttagaataa aaaaaaacat ttatatcaga
     3721 atcaataaat tctatttttt tattactata tcgtcatcat tatcttgacc atttatgttt
     3781 attgttccct gagtggggag tacttctttc aaattcaagt taaatttata ttccattact
     3841 tctcctccaa tatataaaaa ttacttaact gtatccaaat ttaatatgta aataaatcat
     3901 taatttttag ctaaattcta tgagaagttt atttttgttg tttttgctaa ttaactaaaa
     3961 ctagtacaaa aaatttaaaa tagattatta aacctattgc ttattctatt cagtcatata
     4021 tataagcgtc atcctttagt aactaacatc ccacactaca atagtattat aacatatcta
     4081 ttcaacttca aattgttgaa atttaagcca tttatattta cttttcctca actttagaaa
     4141 tgattttact aaccttatat ccgtaatacg cgtaacaaac acattcatta ataacaatca
     4201 acactaagct atattagctt tttaaatgac tagggtaaat aaaataagga tcaaaaactt
     4261 ttaagtttct gaccctttcc ttacttccga ttggttaata gtgatctttt gcagaataaa
     4321 taaatatcgt ttcattttca actctatata tcagtctatg ttcatctgta attcttctgg
     4381 accattttcc agataaatca tgctttaatg gctcaggttt tcctaatcca gcaaaggggg
     4441 aacgatcgat atcttttatt aacttgttaa tcttttttat attgcttttg tttccttgct
     4501 catgccaata aagataatca tcccaagcat catcagacca agccttaatc atcagattca
     4561 acctcgatta agtcatgtgt tttaaatgca cctttggaga attgttcatc tcctcgacga
     4621 attttttcca tgacgtaatt attagaaagt gttctcaacg tttcttgcat agaatcataa
     4681 tctctttttg ataatacaac aactgtatct tctacatctt tacttgttac aataagtgtt
     4741 tcagcatcct cattaacttg tttcatataa ctacgtaaat tttggcggaa atttgaataa
     4801 gctactgctt ccattccttt caccccttat ttatttctat aaaacaattg tacattatat
     4861 tgtacaatta agcaaatgat taatattcta acttcttata attaaaagtg ctatcacttt
     4921 tagtttttag gataaaaagt gacgtcactt tttgagtgct ggtacctcaa atcctgcttt
     4981 ttcttgggca atcaataaat ctaacgcaat acacataatc ctggattcac ttttgccgta
     5041 cttctgcttt agttgctgca attctttttg tctcttttca tccaacgtaa cgggtaaccg
     5101 tttatttcct attgcaattg gcattagtac tctctccttt catcgaaata accctatgaa
     5161 aaatatggct gcaatcatct tgaatagtag actcacaagg gctaatacaa agcgccaaaa
     5221 gatcaaacaa aataccagta ttgccaaaaa tactacgaag tttaaatgca cagctgcagc
     5281 atggcgcata agtatttcgc caacaaatag gactaacaat agaggaatga ttttattcat
     5341 atggatctcc ttctttctct taggttatcg ggtgttgccc gcttatttcg caccacctaa
     5401 gcggcggggg cggttggagc gattggtgcg aaataagaac cattccctta gttactaggt
     5461 tttttgtcct tatttcacac attctcactt ttcggtataa atgattattg cctttttttc
     5521 ctttcaaatc gttgatatgg cattcgttta cggtcaagtt cattttggat ttcttctgta
     5581 tcgtcgtaag tataggtgtc taaaaaatca gttaaagtcg tgtaccagtc gtcgcctggg
     5641 ttatctactt cttctacata ttccctcaaa aatgcttcgg gtacagcgag ttcataatcc
     5701 attcccatta acacgataga ttccgttcca cacgtcaaaa atctagtaaa tacaaggtaa
     5761 taatcgtcta tcgtcgcatc tttgctgttt agctcttcca tttcttcttc cattaattcc
     5821 atgctctcaa aaattgaata atattctgat tctgagtaat agtgtttcct gcaaatcgtg
     5881 tagctgacaa agttttcgac cagctcactc ctttctaggt aaacattcat gatgtatttt
     5941 tctgtttcta gtcgtagaag ctccgttcct ttttttaccg tttcaggacg acgtttatac
     6001 acgtctaaac gattatggct gactgtgacc ttcgtttgac tcgtttcact ttccaaatac
     6061 aattgttctt c
//
LOCUS       DANKEF010000106         6028 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000106 DANKEF010000000
VERSION     DANKEF010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.108"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(68..370)
                     /locus_tag="PWZ48_002723"
     CDS             complement(68..370)
                     /locus_tag="PWZ48_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262334.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="PWZ48_002724"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="PWZ48_002724"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="PWZ48_002725"
     CDS             complement(1127..1900)
                     /locus_tag="PWZ48_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HDL2262335.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="PWZ48_002726"
     CDS             2044..2394
                     /locus_tag="PWZ48_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2262336.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="PWZ48_002727"
     CDS             2375..3091
                     /locus_tag="PWZ48_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDL2262337.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="PWZ48_002728"
     CDS             3091..4539
                     /locus_tag="PWZ48_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HDL2262338.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="PWZ48_002729"
     CDS             complement(4610..5119)
                     /locus_tag="PWZ48_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262339.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
BASE COUNT         2051 a         1050 c         1170 g         1757 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaag
     5161 tgaaagggaa tagactaaga atgagacagc atccactgag cccaataaga gtttgggcaa
     5221 atcccttaag tatatctgtg aaaaaaagat cattttggaa atcgatggta aaaaaagtct
     5281 gttgatccat cggaattgta agtccgctta ccgaagtcaa gatatcacgg atatctggca
     5341 gttcagtagt ctttaagaca agaaaattga tcctttgaag aagcgcttca taaactgact
     5401 ggaagcttac gatccacaat aaaaccaaga tacaacaaag aaagagagga aagaacagct
     5461 gccagaaaat aaaaaggaat aatttggttt tagaaagacc agtcaggtgc caattgatca
     5521 attcctgctt gttttgcctg actgaatagt aaaagaaaaa gataaaaagg aaggcaaata
     5581 gaaaaaataa actgaatatg ccttttatat aaaatcggct agctaattct aaagccggaa
     5641 gaagctctgt agatgcagaa gcgccaataa atgaaagccg atcttggact tgattggcta
     5701 aagccgattg caaatggtaa agagtcaggc atgctatcaa taaaaaagca gaaaagcttg
     5761 aaaataaaca atagagtatt gtctgctttt tgtaataaat taatcgagca gtagcatagc
     5821 ggattatttt catacttttc acctctataa aaagtataga aaatgaatat gaataaagta
     5881 tgaaattcct ttagtgaaat cacgaattaa aaccagaaaa tattttgtaa aaagcaagag
     5941 gttgtgaaga agctgcccta ttctattacc gtagatgcaa gcttttgaac actgtttatt
     6001 cgaaaagagg agcagcgaca ataatttt
//
LOCUS       DANKEF010000107         5958 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000107 DANKEF010000000
VERSION     DANKEF010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5958
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.109"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            416..1570
                     /locus_tag="PWZ48_002730"
     CDS             416..1570
                     /locus_tag="PWZ48_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HDL2262340.1"
                     /translation="MIILKPKKKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYIYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1564..1986
                     /locus_tag="PWZ48_002731"
     CDS             1564..1986
                     /locus_tag="PWZ48_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262341.1"
                     /translation="MVNKPIALVMGLVLVLFLTSCSKKQEIQKTDLIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3156..4124
                     /locus_tag="PWZ48_002732"
     CDS             3156..4124
                     /locus_tag="PWZ48_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262342.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKSDIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQVQSEKAPTSLQEKIKEQKRKLELL
                     QQLEKLEREQNTQNKENNAQKSEINSDIPSELKKDLLDNLQNLFKD"
     gene            4141..4665
                     /locus_tag="PWZ48_002733"
     CDS             4141..4665
                     /locus_tag="PWZ48_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2262343.1"
                     /translation="MTEKTIKELAEELGVSKTAINKKVSDENRKLWFSKIGNKFVINE
                     TGQKAIKSMFFNENENSNQQLVGEKTKTENHKNANQVPELEVLSLLEQQLNQKDMQLQ
                     EKDEQIKQMQKLLDHQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIE
                     KQSKAEKKWWQFWQ"
     gene            5041..5322
                     /locus_tag="PWZ48_002734"
     CDS             5041..5322
                     /locus_tag="PWZ48_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262344.1"
                     /translation="MDILVRNINPKIIARIDKRCQELTTKTGKKWSRNKYLKVLIESD
                     FDRQLMNYKKDQFDLLLEKFIAIQTENTNILNDYVHTNNQLIETLIGGI"
     gene            5324..5740
                     /locus_tag="PWZ48_002735"
     CDS             5324..5740
                     /locus_tag="PWZ48_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262345.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         2261 a         1018 c          966 g         1713 t
ORIGIN      
        1 aagactgctc tcacgagcag cccaaaatga gattatccta ttcatcaaca taaaaatatt
       61 acaaagtgaa gaactagaga aagacaaaaa aagcatatca aatcctctca aaaataacaa
      121 gtctttaaaa aatgacagat aaaaatgcac aaagaactaa aaaagagaaa tcgcgtaaaa
      181 tcaagcatta gaagctagta actagctaga ccaattaaaa atcacaaagt gagcgtctct
      241 atccattggt agaggggcta cttggtctag tcgacattat gttaactagc tcgataaata
      301 acgattatgt tcccaaaaca atattttatg aactataaca aaaatttgtt atcgcatatt
      361 gtatatttta attatataat tataggtgta taccacttaa atttaacaca aagacgtgat
      421 tattttgaaa cccaaaaaga aaatttatct aggtctcctc ttttccttga tgattggaat
      481 ttttagtata ggaattacaa atacctatgc ttcagaaaat tctaatttca atatcaactc
      541 ccaaccaatt gaagaacaag caattaattt tgcgaaaact gattttgaac actccataac
      601 ttcaattctt ttaatggaaa ataacaccga aactgatatt aactatactc ttggttcgcc
      661 atttaaaatg ctcgatggag aaaatacggc aaatagttct attttctttc cagtattttc
      721 agacgctgat aatacgatta aatatattta taccgtcaaa aaaaatacaa atggagaata
      781 ttcaggaagt atctcacaat ttttagcaaa agaacttcaa aatctagtag atagtaatat
      841 aacagataca accttatttt ttcaagataa tagcatttat tattccgata atggacaaat
      901 taaactactt tggaaatcac ctttaagtac agaaaaaaat ggtgtttctt cctttacaga
      961 aaaaaatgat cttatatccg taaatataaa aaataaaatt aaaaatttct ctaaaaaaca
     1021 aaattttcct gcggttaaac gtgtaaaaag tgtctcctcc ggagataact ttattatgat
     1081 taattggcgt atagcagaaa cccaaacaag ttttccgtgg tgtgctgctt acgtagaagc
     1141 tgctatctta tgtaacaaaa cagatgtacg tgctacttca gctagagcca ttatgcatta
     1201 tacctatcct aatttaaaca acagtcaaat tgaatcaaaa agtcttaatc aagatcaaat
     1261 aataaaatat gcaaattact gtggtgttta tcctaaaaaa gtagcacgaa tactaaacct
     1321 ttcagaggtc caaacacaac taagaaaagg aaatgccatt tatatgggaa ctgctggtta
     1381 tggaaattat agtaacagcc gtcatgcact tgcattattt gggtgggtca atcaaggtgg
     1441 ttatcaaact tactatgtgt ggaatccttg gtataactac ccaactgtaa taagtgcaaa
     1501 tgcaggacca gttacaattc ctgtacctgg aggtggtttt acttgggaca ataccattac
     1561 aaattggtaa ataaacccat tgctttagtt atgggtctag tgttagttct tttcttaact
     1621 agttgttcaa aaaaacaaga aatacaaaag actgatctga ttccttttct aggaacttgg
     1681 caaactaaag atcattcaga acgtctagta atcactaatt taaatacttc aaaaaatata
     1741 gaacaatata ccatcttagt aaaagagaac aatcataaaa ttcaaatcat tgataaaaat
     1801 ttaaatataa atgaacaagt tttcatttac aaagcaacaa ataatttact aacactgcgc
     1861 tctaataaaa aacaattaga ttacactttt agtttggatt ctccaaaaaa gctcgtattt
     1921 attttgacaa cgaatgatgg gacagaaggg acagctaaac caattgaatt ttttaaagct
     1981 gattgatgaa tagttgtttg atattacaaa acaataattt caccgaaggt gaaacaagcg
     2041 aaactttaca aaaagtggct agcatttgct agcctttttt gtattgtgga gcgtgggttt
     2101 tgagggggat tcaaggggga gcttgcgccc ctttgacaag cggttatttg caccaaatac
     2161 ggaggtattt ggctagcaaa tatagagctt gccaaacact ttagtgagtt ttgtagaaag
     2221 tacctttaaa ggtactttca cattacgaac gatacgtccg taggacgtac tgaaagggtg
     2281 ggatttatga gcgaactaat gcaaaatttg gctagcaaaa aaagttaaaa aatatatgct
     2341 aataatcgag cgaaatgaac actttttcaa gtgagcgaat ccgccatgac aaagctgaag
     2401 cagtttattt cagctttgtc atggcgtgtg cctagacttt taaaaaagtc ggcacacgag
     2461 caaatatctt tatcaagctc taatttgcgt tttaagacgt ttgaaacgtc taagtatata
     2521 aatatataag agtaggttta agagtgctta aatcgaattt tagagggcat tcagacaact
     2581 ttagggttgt tagggcttgc cctgaccgcc tgtaaggcgc tttaagaaca agataaggga
     2641 tttatactaa gactgtctta aaacgtctca aacgaaggct catagcttct caaagagttc
     2701 cttatgaaca tttttagcaa aggagccgga agcgactgcg cagtataatg tgagagcaga
     2761 gcgatcacac ctttttaagc gactgtaggg agtaagattc ccagttgaga aaaatgcgcg
     2821 aatctgtttg gagaaaaaag aagtggtttt ttaggcggga aattttgtcc ccctctttta
     2881 taatctcttt ttaaacctct ttttaaacct ctttaccgtc ggctatatcc ttactctccc
     2941 aagggaatga cgcttttatc tacatcgttt tgtctgttta tctacatcgt tttgtctgtt
     3001 tatctacatc gttttgtctg tttatctaca tcgttttgtc tgtttgtaca ttgaattagt
     3061 acatcgacga tgttaatata agatgtagat aaacaggcat tttgtatttc ataacgtgta
     3121 tctcttgtgc gagtaagaaa ggagctattt taaatatgtc taatgaaatc gtaaagtatc
     3181 atcatgaatt aaatacaatt ccgttgcgaa aatttacacc agtcgaaatg aatttgttct
     3241 tttctatcgt atcaagaatg cgtgatgtag gtgataaaaa agtacaattt acattcgagc
     3301 aattaaaaga tttaagcaat tataaagcaa ctgcaaatgt tcgattcata gatgatttag
     3361 aaacaacgta tgacaaatta atggatttac gttttggtag aagaagtgct gacggtttgc
     3421 aacgtgaacg ttttgtttta tttaatcaat ttaaaatcga cggaaaatct gatattcctt
     3481 ttgctgaaat acaagttcat gaaaaagcct tacccttact taataatctt gaagaatggg
     3541 tgcgctattc tctccaacaa ttcaatgaat tagaaagcag ttattcaaaa acaatgtttc
     3601 gattactaaa acaatacaga accaaaggtt tcgcttattt ttcaaaagaa gactttcatg
     3661 aattacttga tattcctaaa agttacaacc aaggtacgat tgatcaacgt gtgttcaatc
     3721 caatacgaca agaactaacc gcaatcttta aaggtttaac aatcaaaaag aaatacggaa
     3781 aaggacgggg aaagcctgtc attggctatc aatttacatt taaacctgag ataaaaaaat
     3841 cagacgactt taacaaacat gccccaacaa aagaagaaca aaacaaagca ttagaactag
     3901 ctaaccaaca agttcaatct gaaaaagcac ctacttcatt acaagaaaaa atcaaagaac
     3961 aaaagcgaaa attggaactt ttacaacagt tagaaaagtt agaaagagaa caaaataccc
     4021 aaaataaaga aaataatgca caaaaatcag agataaattc cgatattcct tcagaactaa
     4081 aaaaagactt attagacaat cttcaaaact tattcaaaga ttgaggggtt tttagatttt
     4141 atgacagaaa aaacaatcaa agaacttgct gaagaattag gagttagtaa aacagcaatt
     4201 aacaaaaaag tttctgacga aaatcgaaaa ctttggtttt cgaaaatcgg aaacaagttt
     4261 gtgattaatg aaaccggaca aaaggctata aaatcaatgt tttttaacga aaacgaaaac
     4321 tcaaatcaac aactagttgg cgaaaaaacg aaaaccgaaa accacaaaaa cgccaaccaa
     4381 gttcccgaat tagaagtcct ttctctccta gaacaacagt taaatcaaaa agatatgcaa
     4441 ctacaagaaa aagacgagca aataaaacaa atgcaaaaat tactcgacca ccaacaacaa
     4501 ctcaccctgc aagccaacca acaaatcgag aaactgcagg aacaactaca gttgacttat
     4561 acggaagaat cttcggaaaa taaatcaacg actctttctg aagaaacaga atcgatagaa
     4621 aaacaatcaa aggcggagaa aaaatggtgg caattctggc aataaaacca gcaatttaaa
     4681 ttaataaaaa ttgctggttt tattcttctt tttgcttttg cgagcagagc aacatggacc
     4741 catgttgcga tattttggtg gcgccccaat ccccgttttt tcgttgaatc ttactcggac
     4801 tgcactcgtt ttacagctga tttttctgaa ttttgaaaga gcataaggaa acgtggtatc
     4861 actttcttac gaaaggtgct agataggctg tgcctatcac gtttctcaga aaaaaatttg
     4921 ctatcacttt tttgccattg tggctaggct atgtatagtg ttttggcgaa aaatagctat
     4981 acaaatacta tatttgtatg gtatacataa attatacgct gatacgaaag gaaaatttcc
     5041 atggacattt tagttcgaaa tatcaatccc aaaattattg caagaataga taaacgttgt
     5101 caggaactca caaccaaaac gggcaaaaaa tggagtcgaa ataaatatct aaaagttcta
     5161 atcgaaagtg attttgaccg tcaacttatg aattacaaaa aagatcaatt cgatttatta
     5221 ctcgaaaaat ttattgccat tcaaacagaa aatacgaaca tactgaatga ctatgttcac
     5281 acaaacaacc aactcattga aacacttatc ggaggaatat aacatgagaa aaacttctgt
     5341 tcaaaaacac gtaacccttt atcccagtca cgttgacgtt ttagattcca tcatcgaaaa
     5401 aaatccagac gtcaacaatt attcagaagc cattcgtttt ttatgtatga gttatgaacc
     5461 tgataccaat acaaccacaa ccagtaacca agcaaaactc aatgccatgg gaaaagaaat
     5521 ttctatcatg acagaaatca tggcagaatt tgctgattta tatctaaaag atactggtgt
     5581 tcttatcggt acaaattcta aagtgtatca agaagtgaaa gaaaaaattg aaaatcaact
     5641 caatccttcc cgacgaaatc aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac
     5701 ttctggaaat tttgacccaa caaactttct aaaaagataa aaaaagaaac cgatccctaa
     5761 aaaaattcct cgatttcttt ttagggatca gtcttccagc aagatttgca agttggtacc
     5821 aacttgcgaa aaaaaagaaa aatccgctta tgcaacttct tctttttgta ttttggggcg
     5881 ctaccccaat ccccaagtat cggttattta atagcgcgga aatccaaaga ataggacgta
     5941 ccctgaaaca aaaaaaga
//
LOCUS       DANKEF010000108         5885 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000108 DANKEF010000000
VERSION     DANKEF010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.110"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(75..1169)
                     /locus_tag="PWZ48_002736"
     CDS             complement(75..1169)
                     /locus_tag="PWZ48_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HDL2262346.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            1358..2119
                     /locus_tag="PWZ48_002737"
     CDS             1358..2119
                     /locus_tag="PWZ48_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDL2262347.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="PWZ48_002738"
     CDS             complement(2234..2674)
                     /gene="fabZ"
                     /locus_tag="PWZ48_002738"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDL2262348.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            2894..3649
                     /gene="fabI"
                     /locus_tag="PWZ48_002739"
     CDS             2894..3649
                     /gene="fabI"
                     /locus_tag="PWZ48_002739"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDL2262349.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            complement(3759..4772)
                     /locus_tag="PWZ48_002740"
     CDS             complement(3759..4772)
                     /locus_tag="PWZ48_002740"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HDL2262350.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            4937..5857
                     /locus_tag="PWZ48_002741"
     CDS             4937..5857
                     /locus_tag="PWZ48_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HDL2262351.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
BASE COUNT         1789 a         1175 c         1054 g         1867 t
ORIGIN      
        1 ctataaaaaa acaaaataaa aaagcagaaa taactggcta tttctagcgg attatttctg
       61 caataactaa ttatctattt tttattttca attaatttaa agcttgacca tggtttcaaa
      121 aagtcaagga ggaataattc gtcatcggag attcttccta caacattgac acgtccgtcg
      181 tttttcatat ccttcaaggc gatttgtgtt tctcctttgt attgaccgta cccttcattg
      241 tcaatcagta catccccatg atgcatatct cgcgtattat gaggcgggaa atctttatct
      301 ttgtagtata cacgagtcat cgttgaacga aggatatatt ctgagcgatc ccctcgataa
      361 ctatgaaggt tatcaaacaa gcagattcgt tcgttttctg taataccttc ttccgtatct
      421 actttcaatg ttggataatc cgcgttaaat gcttctgcca tttctttcaa ttcttcttca
      481 cttgcgtacg catttccaat agaaatatca tcaatcaatc ctgtaaggat taaatgcttc
      541 acttgtgtcg caatttcaag atctcggtgg tcttccagcg tgcacaagcc atcttgcgta
      601 ggccaagggc caaactcagc agattgcgaa ttgacgaatg ccatcgtatt caaattatat
      661 tttctgaatt tttctgaaca aaaaacaaag tgttcgtatc ctaaaccaga gtatcgatga
      721 ggataaaaat tatgagaacc taataaatta tccgtgtttg gcgaataact catgatgttg
      781 tcaacgtaat tcgtgcccga actcatattg atctcgattt tgataccaaa aggattgcgc
      841 gtcatttttg cttcttctgc tcctgtgaat ccgatatcta agcggacccc gtaggcaccc
      901 atcttatgga aaaaagataa gtcatcgtaa gaaatctcca actgttcaaa taacgctgga
      961 ttgatatcaa ccattacttc catgcctagg ctattcgcgt aatcaactac ttctttaaat
     1021 tctgaaagaa ctttttcttt atcatcattg atctgcagca aactggtaaa aacccgttta
     1081 tacccatact tatgagctaa atccaagtac gctttgtcct tttcaaatgt tgaccgttct
     1141 ggataaatcg agattcctaa ttttcccata ttaccatttc ctcctgcatc ttttcgtttt
     1201 tgtatccact tacattatag caaacacaaa gtaaatggtc tacacctttt ctttttcttt
     1261 taaaataaat gataaattcc aataatttaa gtaggtattt tccctctatt ttgttatcat
     1321 taaagataac aaaatagtaa attggaaagg aacagttatg aagaaaaata aagttatttt
     1381 aagtattagt ttagcagcag cattgctgtt tgctgctggt tgttctgcaa acaacacgac
     1441 ttcttctagt tcgtctacag aaagttcaaa aagtacatct actgcggaaa gcacgactgc
     1501 cagtagcgtc gatttgaacg cattggaatt accacaattg acgaaagatg tcgccgaaaa
     1561 tgaagatctg gtcgaaatgg tgacgaataa aggcacaatc aaaatcaaat tatttcccga
     1621 acacgcacca aaagcggttg aaaactttat gacacatgca aaagacggtt actacaacgg
     1681 actgactttc catcgcgtca tcaaagattt catgatccaa ggtggcgatc ctaaaggcga
     1741 tggcactgga ggggaaagca tttggggaga aggatttgaa acagagatca acaaccacct
     1801 ttataatata agaggtgcgt tatctatggc tcgctcacaa gatcctaaca gtaacggcag
     1861 ccagttcttt atcgttcaaa atacagatga tatgcatgat ggtcttttaa aagacgacta
     1921 tcctcaagca atcatcgacg cttataaaaa cggcggttat ccttcactag atggccatta
     1981 tactgtcttc ggccaagtga ttgaaggaat ggatgtcgtg gatgctattg caaatatcga
     2041 aacagatagt tcagacaaac caaaagaaga agtaaaaatc gataaaatca atatactgca
     2101 agaagcaaaa caaaaataaa aaaataacgt ctgaatcctg catagatgac aacgtatgct
     2161 aggattcagg cgtcttcttt ttacataaaa agcaagtaag cttccttttg ttttgctata
     2221 tcaggtttta tagtcaagca ttttggttga cgatgaacgt gaactcgcac tcacagactt
     2281 ttttatcatc tacatatgct gcagataacg ctgtaccgac tgatcccttc attttggtga
     2341 tttcaaaatg acatttcagc acatctcctg gaacaacttt ttgacggaat ttggccttat
     2401 tgatcccacc gatataagct ctttttccta gaaactcttc tgattttagg ataagtatcg
     2461 aacctgcttg tgccagtgtt tctagaatca acgcacccgg catcactgga ttgtcaggaa
     2521 agtgaccttg gaaaaaatct tcattgattg ttacattttt tgtcgcaacg atcttttttc
     2581 ctggttctaa agtatctaca taatcaatat aacaaatagg ataacgatta ggaatcaatt
     2641 ccattacttc agtagcagat aatacgtaag acataattaa actccttctt tcaggcaatt
     2701 gccattttca ttactttgac tatcaaagta tcatattcct aaatatttag caatcttttt
     2761 ttaatttaag aaaaaatagt ttgacaatca aaacattttg actaataatt aagaaatcaa
     2821 gaagttattt tagctattat tctctatttt tttacgacta tcaaattttt atcagaaaca
     2881 aaggagagtc attatgtcat ttctagaagg aaaaaaaatc gtcattatgg gcgtcgctaa
     2941 caaaaaaagc attgcttggg gttgtgctga agcaatgttg aaacaagggg cagaaattat
     3001 ctatacttac caaaatgaac gaatgaaaaa atcattagtc aaattggtcg gcgaagaagc
     3061 attcactgta gaatgtgatg tttctagcga cgaaagcatc gaaaaagcaa tgaatgccat
     3121 cggtgaatat gctggggaga tccacggcct tgtccatgca gttgcttacg ccaacaaaga
     3181 agaattgtcc ggaaatgtgt ctgatatttc cagagacggc tatcaattag ctcaagatat
     3241 cagcagctac tctttaattg ctgtatcaaa atacgcacaa ccgtttcttg caccaaacag
     3301 cgggatcgtt agcatgactt atctaggttc agaacgcgca atcccgaatt ataacatgat
     3361 gggtattgcg aaagcttctc ttgaagctgc tatccgttat ttggcagctg agttctcgcc
     3421 taaaggaatt cgggtcaatg gaatctcagc tggggcaatc aaaacattag ctgtcacagg
     3481 tgttaaagac tatcaaaaac tgatccagtt atctgaatct cgtacgcctg atcagcaagg
     3541 tgtgacgatc gaagaagtcg gtaatgcttg tgctttctta atcagcccac tttccagcgg
     3601 aatcatcggt gatattatct acgttgacaa aggtgtccat ctttcataaa cttttgttgc
     3661 attctttaaa gtaaaacata taaaaatcag gaaaaaactt tttaaagtct cttcctgatt
     3721 ttttcttttt acgttttttt caactcatta ctttgaaatt attccacgac ttcaatccag
     3781 ccttctggtg cttctatgtc tccaaattga atacctgtca attcatcata caaccgtttt
     3841 gttactggtc ctacttccgt ttcactataa aatacatgaa gattgttatt gtgataaatc
     3901 ccaccgattg gagaaataac agccgctgta ccacatgcac cagcttctgc aaattgatct
     3961 agatcatcaa tgaaaatatc tccttcaatt gcttgcagtc ctagtcgttc ttcagctaac
     4021 tgcaacaagg aatatttcgt gatacttggt aagatagagg gcgattgcgg agtgatgaat
     4081 tttccgtctt ttgtaattcc aaagaaattt gctgcaccta cttcttcgat tttcgtatgt
     4141 gtggctggat caagataaac acaatctgag taatccagtt cttttgcttc tgttcctgga
     4201 agtaaactcg ctgcgtaatt tccgccgact tttgctgcac cagtcccctt aggagcagca
     4261 cgatcgtaat cagatacaat aaaattcgtt ggttttagtc cacctttgaa atacgctcct
     4321 actggcgtac agaagacgac aaataaatat tctggagctg gatggacacc aatgttttca
     4381 ccgaccccaa tcagtaaagg gcgcaagtat aatgtagcgc ctgttccata gggcggcaca
     4441 aaatcttcat ttgcttttac cacttgcttg acagcatcaa cgaacatctt ttcagggacc
     4501 attggcatca gcaatcgtct agcactttga ttcatccgtc gactattttg ttcaggtcga
     4561 aagagattga ccttgccgtc ttttcgacga taagctttta gaccttcaaa acattgctga
     4621 ccataatgaa gcgctggaga gccttcatga atcgtcaggt aattatcttc tgtcaattct
     4681 ccttcttccc attgtccgtc tttccatttg gcaataaaac gccaaggagt tttgatataa
     4741 gaaaaaccta gattgttcca atcgatttgc atatacttac caccttcgtt attttctcca
     4801 cattttatca ttatccatat tatttgtcat tttattttca gcttttttcc ttcattatag
     4861 ttatttttgc tatttccttt tttgaaaact tcttggtaca atacagaaag aatcaaattt
     4921 tagaaaagag gattttatgt tttcattatt agcaactgcc ggtacgacag actcttctgg
     4981 cgagacattg acagatcaag ctgtcagcaa agtaaatgcg tttcagcggt tttgggacgg
     5041 gattgattgg gacgccattt tggctgcctt tatccaaaaa agtttatatc tgattttttt
     5101 gatcgtcctt ttttggattc ttcaccgtgt gggaaaatat ctgattgata agtcttttaa
     5161 tcagtattct aaaaaagcca ttctaaacga gagccgctta aagactttac atagtctttt
     5221 aaatactatt tttcattata cccttggttt cttttttatc tatgcaattt tatctgctat
     5281 cggtgtgccg atcggatcac ttttggctgg tgccggaatt gcaggtgtgg caatcggtct
     5341 tggtgcccaa gggttcatga gtgatgttat taccggattt tttattatca tggaacaaca
     5401 gatggatgtt ggggactaca tcaagctagc aaacttaacg attgaaggaa ccgtcgtatc
     5461 tgttggtatc cgtacccttc agctgaaagc aacggatggt actgtccatt tcatcccgaa
     5521 tcgaaacatc accacgatca gcaatctctc tcgtgcaaat atgcaagtac ttttagatat
     5581 tcggattgtt cctgaagaag gatatgacaa aatctatgag atcattgatc gagtaaatcg
     5641 tacattagct gaaaaatacc aagaagagct ccaaactgaa ccttctatct ttggattagt
     5701 ggatcttgga aacagcaatt ttgcgattcg tacggtctgc tacgcattga acggaaaaca
     5761 atatgcctta aaagaagagt tcttgagctc ctatgtcaaa gaattgacag cttctggttt
     5821 cacgattccg aacagtccaa ttgctgtcgg aaaataattt ggacaaacga tgcaacgaat
     5881 ctcac
//
LOCUS       DANKEF010000109         5640 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000109 DANKEF010000000
VERSION     DANKEF010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.111"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            364..534
                     /locus_tag="PWZ48_002742"
     CDS             364..534
                     /locus_tag="PWZ48_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262352.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            548..1150
                     /locus_tag="PWZ48_002743"
     CDS             548..1150
                     /locus_tag="PWZ48_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262353.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1166..1843
                     /locus_tag="PWZ48_002744"
                     /pseudo
     CDS             1166..1843
                     /locus_tag="PWZ48_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2154..2318
                     /locus_tag="PWZ48_002745"
                     /pseudo
     CDS             2154..2318
                     /locus_tag="PWZ48_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            2355..2651
                     /locus_tag="PWZ48_002746"
     CDS             2355..2651
                     /locus_tag="PWZ48_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HDL2262354.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2652..3068
                     /locus_tag="PWZ48_002747"
     CDS             2652..3068
                     /locus_tag="PWZ48_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HDL2262355.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3088..4632
                     /locus_tag="PWZ48_002748"
     CDS             3088..4632
                     /locus_tag="PWZ48_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262356.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4775..4999
                     /locus_tag="PWZ48_002749"
     CDS             4775..4999
                     /locus_tag="PWZ48_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2262357.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5014..>5640
                     /locus_tag="PWZ48_002750"
     CDS             5014..>5640
                     /locus_tag="PWZ48_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2262358.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQV"
BASE COUNT         2011 a          898 c         1208 g         1523 t
ORIGIN      
        1 ttgagctatg tacaagaagc attttattct agcttaattt gacaaatcat actatgatac
       61 gtgtttgttt ttctgtgctg tttagtgaat gattagcaga aatatagaga gtaagatttt
      121 aattaattat tagggggaga aggagagagt agcccgaaaa cttttagttg gcttggactg
      181 aacgaagtga gggaaaggct actaaaacgt cgaggggcag tgagagcgaa gcgaacactt
      241 gatcttttaa gttgctatct ttttaaggtc aatagagtat acttatttgt cctaatatgg
      301 ttttagcagt ataattgact tggtgaatag gtcatttaag ttgggcataa taggaggagt
      361 aaaatgaaaa aatttattta tcgagtttta gaaaatgacg aagtggtggc tatttttaat
      421 gagcaacaat atgcgcaaga ttttatcgct tacgaaaaga caatttctga taagcaattt
      481 gaaattgaaa aagtagatat agctgattgg ttattgcaac cgagagaatt ttagaggttg
      541 gttgaaaatg gctaaaattg gttatgcacg tgtcagtagc aaagaacaga acttagatag
      601 acaactgaaa gcgttagagg gcgtttctaa ggtcttttca gacaaattaa gcggtcaatc
      661 ggtcgaacgc ccacaattac aagctatgct taactatatt cgtgaagggg atatagttgt
      721 tgttactgaa ttagatcgat taggacgaaa taataaagaa ttaacagaat tgatgaatca
      781 aattcaaatt aagggggcaa ccctggaagt cttaaattta ccctcaatga atggtattga
      841 agatgaaaat ttaagacggc tgattaataa tttagtgatt gaattgtata agtaccaagc
      901 ggaatctgaa cgcaaacgaa ttaaagaacg ccaagcccaa ggaattgaaa ttgctaagaa
      961 aaaaggaaaa ttcaaagggc gacaacctaa attcaaagaa aatgatccac gtttacaaca
     1021 cgctttcgat ttatttttga atggttgttc cgataaagaa gttgaagaac aaactggaat
     1081 taatcgccga acgtttagaa ggtatcgatc aagatacaac gtgacagtag atcaaagaaa
     1141 aaaacaatga aaagagggat agttaatgag tacggttatt ttagctgaaa aaccaagcca
     1201 ggcattagcc tacgcaagtg ctttaaaaca aagcaccaaa aaagacggtt attttgagat
     1261 caaagaccca ctatttacag atgaaacgtt tatcaccttt ggttttgggc atttagtcga
     1321 attagcagaa ccaggtcatt ataacgaaaa gtggcagaac tggaaacttg aatctttgcc
     1381 gatttttcct gatcgatacg attttgaagt tgcaaaagat aagggaaagc agtttaaaat
     1441 tgttgctgaa cttctcaaaa aggcaaatac aattattgtc gcaacagata gcgacagaga
     1501 aggtgaaaat atcgcctggt cgattatcca taaagcaaat gccttttcta aagataaaac
     1561 gtataaaaga ttatggatca atagcttaga aaaagatgtg atccgaagcg gttttcaaaa
     1621 tttgcaacca ggaatgaatt actatccctt ttatcaagaa gcgcaaacac gccaaattgc
     1681 cgattggttg atcggcatga acgcaagccc tttatatacc ttgaatttac aacaaaaagg
     1741 cgtacaaggt acattttcat taggacgtgt acaaacaccg actttatatc tcatttatca
     1801 acgccaggaa gcaatagaaa actttaaaaa agaacctttt ttcctgaata attcatagtt
     1861 ttactaatag tctttcctaa cattgattta atagtcttta aagtattctg cttcatcacc
     1921 cattaggtga tgaaaaaaga acccctttct gatttagcgg acaaatactt tgacctaatc
     1981 tcagaagata taaaagaaaa aataataaaa tatccgattt atactttgta taaagaacta
     2041 aagtagttag caaatcattt acagcaaatc actatgttcc acataccatt ttacatactt
     2101 accttatcca cgacacatgt tgagatagta tcgaagataa agaaagttgc tccatgttgt
     2161 ttttgcgaga tggggaaatc aaagccacat tgacaaccat gatgaataag cttgctttca
     2221 gtcataagct aatcctagaa ccacttttca agtcagttag ccaaatcgat gaagaaagcg
     2281 accgtgaaag aatggacgct attgataagc taatggagca actattggaa gaacgcaata
     2341 cccttattgc actgatgtcc aaaggtttcc tagaacctgc tctttttaat caggaacgaa
     2401 atgttttaga tagtgagatt aaaaatctta ctaccgaaaa aacaaacctg gtaacgaact
     2461 ctgcgagtgg ggttttgcga gcaaacgaga taaaggacct cattaattat gtgtcagcag
     2521 ataattttaa tggtgactac acggaagaac tatttgaaga atttgtagtg aacatcattg
     2581 taaattccag ggatgagctg acattcaatt tgaaatgcgg tctttccctg aaagaaaagg
     2641 tggtgagata aatggcatat attccatatg gatacaaaat tcaagatgga gtggttactg
     2701 tcgatgaaaa ggcagcaggt caagtaaagg tattctttga gaaatacata tcaggactat
     2761 cccttacagt ggctggcgaa caggcaggta ttgaaaagac acattctgtg atggggcgca
     2821 ttttgaaaaa cgtcaactac cttggaaatg atacgtatcc agcaattatt gataaagaga
     2881 tatttgataa agctgaagaa gttagagata aacgtgcaaa ggatttagga cgagtggtag
     2941 agcttgccgc tttcacctct ccccctccca aagaacgatt caaaatgaaa aaggcagata
     3001 ataagatgcc agtggatcct tttgaacgag cagaatactt atacagtctg atagaaagcg
     3061 aggaataaag tgacagagaa aaatataatg gttattcctg ctcgtaaaag agtaggaagt
     3121 acagccgcaa aagaaaagat aaagaaactt cgtgttgctg cctattgccg tgtttctaca
     3181 gaaacagaag aacaaaattc tagctatgag gttcaggtct cacactatac agagtttata
     3241 aagaaaaata atgaatggga gtttgctggc atctttgcag atgatggtat ctctggtacg
     3301 aacactaaaa agcgtgacga atttaatcgc atgattgcag agtgtatgga cggtaacatc
     3361 gacatggtta ttacaaaatc catcagccga tttgcgcgta acaccctaga ctgccttcaa
     3421 tacattagac agctcaagga taagaacata tcagtttatt ttgagaaaga gaacatcaac
     3481 accacggatg ccaaaggtga ggttttgctg actattatgg catctttggc acagcaggaa
     3541 agccagagcc tttcacaaaa cgttaagctt gggctacagt accgatacca acaaggaaag
     3601 gtgcaggtca accataagcg atttatgggc tacacgaaag atgaagatgg aaatttaact
     3661 attgtccccg aagaagctga gattatcaaa cgcatctacc gagaatacct tgagggtcag
     3721 agtctagtgg gcattggtcg agcccttgaa aaggatggta ttttaacagc agcgggaaaa
     3781 ccaagatggc gaccagaatc agttaagaag atacttcaaa acgaaaaata catcggagat
     3841 gcccttctgc aaaagactgt cacagtagat tttctgacca agaaacgagt gaaaaatgaa
     3901 gggcatgttc cccaatatta tgttgaaaat agccatgaag cgattattcc taaagagtta
     3961 tttttgcagg cgcaagaaga aattcatcgg agaagcaata tctacacagg agaaggcaag
     4021 aacaaaagaa tttacagtag caagtacgct ttaagtgcca tcaccttctg tggagattgt
     4081 ggcgatattt atagacgggt ctattggaat atccacggaa gaaaagaatt tgtctggcga
     4141 tgcgtgacta gaatcgagca aggtcctgaa gtttgtaaga accgaaccgt aaaagaagat
     4201 gaactttatg gtgcagtaat gactgcgatt aataagctac ttgcaggtgg gaacaatatg
     4261 ataaagacac tggaagagaa tattcatgct gtgattggcg aaaccacaga ataccaaatt
     4321 tcagagatta acaactcact agaggaaaaa caaaaagaac taatcaagct ggcgaacaag
     4381 ggtcaagact atgaccactt agcagatgag attgatgagc tgagagacaa gcgacagatc
     4441 cttttagtag aagatgcctc cctcagtggc gagaacgagc gaatcaatga gctgattgaa
     4501 tttatccgca agaacaaatt ccgcacctta gaatatgatg ataagcttgt aaggaagata
     4561 atccagagcg ttacagtcta tgaaggccac ttcgtcatag ccttcaaatc tggcatcgaa
     4621 atggaaatat gaaaaccaga agtaaatagc ccatgactct acagtagagt tgtgggtttt
     4681 cttttgcttg ctgattataa taatatttta ataactattg tgtttatcaa ccaaatggtt
     4741 tataatattc ttagcgtaaa cttggaggtg ctgtatgact acggcagaaa tgattaaaga
     4801 actgtgtgag caaatgaata taagtgtttc cgaacttgct agacgtattg gccagactcc
     4861 acagaatttc aataaaaaat tacaacgaga aacagtaacc ttggatgagt tgaaggccat
     4921 tgctgatgtg ctaggtgtca agtttgtgca ggcatttatt ttaccagatg gagaagaaat
     4981 aaaaatatct aacgaataaa ggaggatgtt tatatggtag gtaatgttat tcaaatagta
     5041 acagagaaat tatcatcttt gccttttata gaaggcatcg tattaggggg ctcacgtgca
     5101 agaggcaccc atacagaaaa ttctgatata gatatcggaa tctattacaa ttcagactca
     5161 tttgacctga cagcgattaa tcaaattgct acagagttgg atgatgagaa tagaaacaac
     5221 cttgttgtac ctcccggagc gtggggtgat tgggttaatg gcggtggatg gttagttata
     5281 aacgggtgtc atgttgactt gatattacgt gatataaaac gggtggaaca aataatcaaa
     5341 gatacggagc aaggaattgt tactgccaat tatcagaccg ggcatcccca tggttatata
     5401 agtgctatgt atcgtggaga attagcgatt agtaaaatac tatatgctaa gaacgaaagc
     5461 ttatgcgaat taaaaaagca ggcagaaatt taccctactg ctctaaagaa aagcttgatg
     5521 aactttttta tatttgaagc tgaattctct ttaatgtttg taaaagcaaa tgcgggggca
     5581 gaggataaat attatattgc aggccatgtt tttcgtatta tttcatgctt aaatcaagta
//
LOCUS       DANKEF010000110         5466 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000110 DANKEF010000000
VERSION     DANKEF010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.112"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            118..1107
                     /locus_tag="PWZ48_002751"
     CDS             118..1107
                     /locus_tag="PWZ48_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2262359.1"
                     /translation="MRDKEMRQLHKEQIETAMAALKEKTIDHVYFTACGGSQAVLMAG
                     QYLFDKELTVPCHVYTANEFNYAIPKGLSENSLVITCSHSGTTPETVEATKKAREFGA
                     MTICLSNEEGSPLWEAAEYPVHYDWGKDVDASDLNKGILYGLLFNILLVLDGSKKWET
                     CLNEFDKLNDLVIAEKSKFEITAKEWGKNQKRDKIIYTIGSGINYGEIYSTAACWFME
                     MQWINSNAIHSGEFFHGPFEITDYDVPFILVKSIGDTRFLDQRVEDFTKKFSNKLMVL
                     DQADFDLESIDSVAKEYIAAVLSGVVIRQMVEAIAFERGHSLDVRRYMWQMKY"
     gene            1089..1550
                     /locus_tag="PWZ48_002752"
     CDS             1089..1550
                     /locus_tag="PWZ48_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2262360.1"
                     /translation="MANEILINRRLQKMANQLFHIVITTHSTLCEGYLKATKLILMND
                     ATGISAVPFKENTSPDDFEESMKEIISQHHLQPLIILTDLIGGTPNNVSIKQLNNNRI
                     QIIAGINLPLLLELLMAQSAGKSIEEIDFKELLNNSQKSMVHINSLLKDVS"
     gene            1600..2064
                     /locus_tag="PWZ48_002753"
     CDS             1600..2064
                     /locus_tag="PWZ48_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2262361.1"
                     /translation="MIKLVRVDHRLLHGQVIFSWTKQYDINHIIVADNKVPGDPMSVM
                     ALTIAKPADCKLDIVKISDVKGIMETSPNDNYMILIKGPKEAFELVQNLPEIKEINFG
                     GVSKKSESKQYGKAVFLTPEELEATKKILDQEVKIFVQQVPSSSIENVDFSK"
     gene            2078..2827
                     /locus_tag="PWZ48_002754"
     CDS             2078..2827
                     /locus_tag="PWZ48_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2262362.1"
                     /translation="MLLKAILLGLVGVVAVIDSRLIGRQNIGRPLILSTLAGLVLGDL
                     HTGIVLGASLELISMGFVSIGAAGPPNMQLGSIIATAFAILTNSSTETALTIAIPVAV
                     AGEFMSIIMRMIIAQFGHAADKAIENNKFRKAQIIHIYWSFIFNAFVYFIPIFLTVYF
                     GADVVADLVDKIPEMITDALTVSGNMLSALGFAMLLSTMLSKKLYPYFIFGFFIVAYS
                     GLSLIGVTIFAALIAFVMDQVKYGKREEAHG"
     gene            2827..3657
                     /locus_tag="PWZ48_002755"
     CDS             2827..3657
                     /locus_tag="PWZ48_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2262363.1"
                     /translation="MENSTEKKRLITKKDLHKMCLRSWTIMCSWNYERQMHMGFMYGM
                     APILNKVYKNDEEKKREAYTRHMAFFNCTPQMTPFIMGLCASMEEQNAQAEEGNFNTE
                     SISMIKTSLMGPFAGIGDSFFQGTLRIITFGIGLSFAKQGSILGPILAVGLFAIPSIL
                     AVFYGGKLGYESGNKYLTKLYQEGLMDRVMQFASIIGLAVVGGMVASMVPIVTPLKFT
                     TGGTELVLQDLLDSIIPGLLPLVCTLGIYKLIKKNVSTNMLLLGCILAGFVLSIIGIL
                     "
     gene            3657..4304
                     /locus_tag="PWZ48_002756"
     CDS             3657..4304
                     /locus_tag="PWZ48_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2262364.1"
                     /translation="MIKALIFDMDGVLIDSEIMYQKWVFNFLKQENLSYPLYSYQKKI
                     GTTASIFDDIEKYNEGCNSLELKGKFTKYWKSQKIDYLDIFPNILIEQLEWLKKKKIK
                     MAIASSSPMDTINEVVTTCKIESYFDCLISGRDLPESKPNPTIFLKASEQLRIPVEEC
                     LVIEDSYNGIKAGKRANMKVLAIKDKRFSQDVSLADDVIYDIKYLRQEVQVKFGI"
     gene            complement(4402..5178)
                     /locus_tag="PWZ48_002757"
     CDS             complement(4402..5178)
                     /locus_tag="PWZ48_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2262365.1"
                     /translation="MNFQDNRPLYLQVEASIRNDIREKKYLPGEQLPTEEELCKIYGV
                     SKITIRKAFKLLTESGTVERLRGKGTFVTQKKESLKLAGVHGFSDSLSTRGHKIRYTV
                     LHSKIIKADEFLANKLQINVTDRVYNIKRLMWEDGAPMGIDNFFASEEKFPTLFEKGS
                     QYTSLYKLLEDDYDVKATSSIMEINGLSATPELSDLLQCIVGDPLFILEKVGKDKNNQ
                     PFHYSQSTVRCDRISYVIEVTENITLDTVHKNDSDPKVID"
     gene            5260..5403
                     /locus_tag="PWZ48_002758"
                     /pseudo
     CDS             5260..5403
                     /locus_tag="PWZ48_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608918.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
BASE COUNT         1872 a          739 c          973 g         1882 t
ORIGIN      
        1 attttattat ataaaattgt tataaaaaat tttttttgaa aaatagtatt gcaaaataaa
       61 gcaatttcat agataatagg tatcgtaaag ttatataact ttataatacc tattagagtg
      121 agggataaag aaatgagaca actacacaaa gaacagattg aaacagcaat ggcagcgtta
      181 aaggaaaaga caattgatca tgtatatttt acagcatgtg ggggttcaca agccgtttta
      241 atggctggtc aatatctatt tgataaagaa ttaactgtac cttgtcatgt ttatactgct
      301 aacgaattta actatgctat accaaaagga ttatctgaaa attctttagt tatcacttgt
      361 tcccattctg gcacaacacc agaaactgta gaagcaacaa aaaaagcaag agaatttgga
      421 gcaatgacta tttgtttatc taatgaagaa ggatctcctc tatgggaagc agctgaatat
      481 cctgttcact atgattgggg taaagatgtt gatgctagtg atttaaataa aggaattctt
      541 tatggtttat tatttaatat tttacttgtt ttagatggct ctaaaaaatg ggaaacttgc
      601 ttgaatgaat ttgataagtt aaatgatctt gttattgctg aaaagagcaa atttgaaata
      661 actgctaaag aatggggtaa aaaccaaaaa cgagataaaa ttatttacac aattggtagc
      721 ggtattaatt acggagaaat ttactccaca gcagcttgct ggttcatgga aatgcaatgg
      781 attaattcta atgctattca ttctggggaa tttttccatg gtccttttga aatcactgat
      841 tatgatgtac cattcatctt agtgaaaagt attggagata caagattttt agatcaacgg
      901 gtagaagact tcactaaaaa attcagtaat aaattaatgg ttttagacca agctgatttt
      961 gacttagaat ctatagatag tgtagcgaaa gaatatattg cagctgtact tagtggcgtt
     1021 gttattcgcc aaatggtaga agcgattgct tttgaaagag gccattcctt agatgttaga
     1081 cgctatatgt ggcaaatgaa atattaatta ataggaggtt gcaaaagatg gcaaatcaat
     1141 tatttcacat tgtgattaca acacattcta ccctttgtga gggttattta aaggcaacaa
     1201 aattaatttt aatgaatgac gccacaggta ttagtgctgt tccatttaaa gaaaatacta
     1261 gtccagatga ttttgaggag agtatgaaag aaataattag tcagcatcat ttgcaacctc
     1321 taattatttt aacggattta attggaggaa cacctaataa tgtttctatt aaacagttga
     1381 acaacaatag aattcaaatt attgctggga ttaatttacc tcttttactg gagttattaa
     1441 tggcccagtc agctggtaaa tctatagaag aaatagattt taaggaatta ctgaataact
     1501 ctcaaaaaag tatggttcat attaattcgc ttttaaaaga tgtcagttaa ggtggcaatt
     1561 tctattctat aaattttaaa aattgaagga ggaaatagaa tgattaaatt agtgcgtgta
     1621 gaccacagat tacttcatgg acaagtaata ttttcttgga ctaaacagta tgatattaac
     1681 catattattg tggctgataa caaagttcca ggagatccaa tgtctgtgat ggctttgaca
     1741 attgctaaac ctgctgattg caaattagac attgttaaaa ttagtgatgt taaagggatt
     1801 atggaaacat ccccaaatga taattacatg attttaatta aaggacctaa ggaagctttt
     1861 gaattagttc aaaatttacc agaaattaaa gaaattaatt ttggcggtgt gtctaagaaa
     1921 agcgagtcca aacaatatgg aaaggcagtc tttttaactc ctgaagaatt agaagccaca
     1981 aaaaaaattc ttgatcaaga agtgaaaata tttgtgcaac aagtcccatc ttcaagtatt
     2041 gaaaatgtag attttagtaa gtaagggggg aaaataaatg ttattaaaag ccattttatt
     2101 aggtttagtt ggtgtggtag ctgttattga ctccaggtta attggtcgac aaaatattgg
     2161 tagaccacta attttgagta cacttgccgg acttgtgcta ggtgacttgc atacagggat
     2221 tgttcttggg gcatctttag aattaatttc tatgggattc gtctcaattg gggcggctgg
     2281 acctcctaat atgcaacttg gtagtattat tgccactgct tttgcaattt taactaatag
     2341 tagtacagaa acagccttaa caatagctat cccagtggct gtagctggtg aatttatgag
     2401 tattatcatg cgtatgataa ttgctcaatt tggacatgct gcagataaag ccattgaaaa
     2461 taataaattt agaaaagctc aaattattca tatttattgg tcatttattt ttaatgcttt
     2521 tgtttacttt attccaatct ttttaacagt atattttggt gctgacgttg tggcagattt
     2581 agtagataaa attccagaaa tgattacaga cgccttgact gtctctggaa atatgctttc
     2641 cgcattaggg tttgcaatgc tattaagcac aatgttgagt aaaaaattat atccttactt
     2701 tatttttgga ttctttattg ttgcctattc aggtctttca ctaattggcg tgacaatttt
     2761 tgcagctctg attgcctttg taatggatca agttaaatat ggaaaaagag aagaggcgca
     2821 tggttaatgg aaaatagtac agaaaaaaaa cgtttaatca caaaaaaaga tcttcataag
     2881 atgtgtcttc gttcatggac aatcatgtgt tcgtggaatt atgaaagaca aatgcatatg
     2941 ggatttatgt atgggatggc gccaatttta aataaagttt ataaaaatga tgaagagaaa
     3001 aaaagagaag cttatactcg tcatatggca ttttttaact gtacgcctca aatgactcca
     3061 tttattatgg gactttgtgc ttctatggaa gaacaaaatg ctcaagcaga agaaggaaac
     3121 tttaatactg aatctatctc catgattaaa accagtttaa tgggtccttt tgctggaatt
     3181 ggtgattcat ttttccaagg tactttaaga attattacat ttgggattgg attgtctttt
     3241 gctaagcaag gtagtatttt aggaccgatt ttagctgttg gcctttttgc tataccttct
     3301 attttagctg ttttttacgg cggtaaatta ggatatgagt ctggcaataa ataccttact
     3361 aaattgtatc aagaaggtct tatggatcga gtaatgcaat ttgcttctat cattggtttg
     3421 gctgtagtag gtggaatggt ggctagcatg gtgccaattg tcaccccttt aaaattcact
     3481 acagggggaa cagaacttgt tttacaagac ttacttgatt ccattattcc tggattatta
     3541 ccacttgtat gtactttggg aatatataaa ttgattaaga aaaatgttag tacaaatatg
     3601 ctcttattag gttgtatttt ggcagggttt gttttaagta taataggtat cttataatga
     3661 ttaaggctct aatttttgat atggacggag ttttaattga tagcgagatt atgtatcaaa
     3721 agtgggtatt taatttttta aaacaagaaa atttaagcta tcctttgtat tcttatcaaa
     3781 aaaagattgg aactacagca agtatttttg atgatataga aaagtacaat gagggatgta
     3841 acagtttaga attgaaggga aaatttacta aatattggaa atcgcaaaag attgattact
     3901 tggatatttt tcctaatata ctaattgaac agcttgaatg gttaaaaaag aaaaaaataa
     3961 aaatggcaat tgcttcttcc agtccaatgg atactataaa tgaagttgtt accacttgta
     4021 aaatagagtc atattttgat tgtttaatta gcggaagaga tttgccagaa agtaaaccta
     4081 atcctactat attccttaaa gcttcagaac agctcagaat tccagttgaa gaatgtctgg
     4141 tgatagaaga ttcttataat ggtataaaag ctggtaaaag agcaaatatg aaggttcttg
     4201 caattaaaga taaacgtttt tctcaagatg taagtctagc agatgatgtt atttacgata
     4261 ttaaatatct gcgacaagaa gtacaggtga aatttggaat ataaataagt tttttgatgt
     4321 atttagagaa tattagtaaa aataaaaatc atgcccaaat ctaatcaaaa ttgattgctt
     4381 tgggtatgat ttcttttaat atcaatctat tactttagga tctgaatcat ttttatgcac
     4441 cgtatctaaa gtgatatttt ctgtgacttc aataacataa ctaattctat cacaacgaac
     4501 tgtggattga gaataatgaa atggttgatt atttttatct ttgccaacct tttctaaaat
     4561 aaatagcgga tctcctacta tacattgcaa taagtcggaa agctcaggtg ttgcagataa
     4621 accgtttatt tccataatag agctcgtagc tttgacatca tagtcatctt ctagaagttt
     4681 atataatgaa gtatattggc tacctttttc aaataaagtt ggaaattttt cttctgatgc
     4741 gaaaaaatta tcaattccca tcggggcacc gtcttcccac attaaacgtt taatattgta
     4801 aactctatca gttacattga tttgtaattt attagctaaa aactcatcag cctttataat
     4861 tttagaatgt aaaacagtat atctaatttt atgtcctcgg gtagaaagag aatcgctaaa
     4921 tccatgaact ccagctaatt ttaaactttc ttttttttga gttacaaagg tccctttccc
     4981 ccttaatcgt tcaactgttc cactttctgt taaaagttta aaagcttttc gaatggtaat
     5041 cttacttact ccataaattt tacataattc ttcttctgta ggtaattgct ctcctggaag
     5101 atattttttt tcacgaatat cgttacgtat agaagcttct acttgtagat ataatggtct
     5161 attatcttga aaattcatag tacactccct aaaaagttat acttcattat atcacaattt
     5221 attaaaataa aagattaaga tataaatata aatgaactag tctttgacta accaatgccc
     5281 gcttttgaaa gaaaacaaat tcattctgtt ttaagtaaaa atccttatgt taccactcat
     5341 tctgaagggg atgagcctta tcgctacttg gttgtggaac caagcgataa gacaatttta
     5401 taaattcaat attaattaaa ataaaaacaa acaaagtaga ttattttact ttgtttgttt
     5461 ttttat
//
LOCUS       DANKEF010000111         5373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000111 DANKEF010000000
VERSION     DANKEF010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.113"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(560..1600)
                     /locus_tag="PWZ48_002759"
     CDS             complement(560..1600)
                     /locus_tag="PWZ48_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2262366.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(1949..2275)
                     /locus_tag="PWZ48_002760"
     CDS             complement(1949..2275)
                     /locus_tag="PWZ48_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262367.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2262..3065)
                     /locus_tag="PWZ48_002761"
     CDS             complement(2262..3065)
                     /locus_tag="PWZ48_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2262368.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3418..3708)
                     /locus_tag="PWZ48_002762"
     CDS             complement(3418..3708)
                     /locus_tag="PWZ48_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HDL2262369.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3844..4023)
                     /locus_tag="PWZ48_002763"
     CDS             complement(3844..4023)
                     /locus_tag="PWZ48_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262370.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4020..4370)
                     /locus_tag="PWZ48_002764"
     CDS             complement(4020..4370)
                     /locus_tag="PWZ48_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262371.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4363..>5373)
                     /locus_tag="PWZ48_002765"
     CDS             complement(4363..>5373)
                     /locus_tag="PWZ48_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HDL2262372.1"
                     /translation="YVADEDLLIYSIDESILKVTRSLNLFTTEGTRSQRRKKLAQMIQ
                     ENIKEELGLIATVGVGDNPLLAKLALDNEAKHNEGFVAEWTYENVPEKVWNIPEMTDF
                     WGIGSRMKKRLNQMGIMSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPE
                     PTKEKSYGNSQVLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILE
                     TRPGFSHQMKIPITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLF
                     SDPQKQIDEENLDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIK
                     ND"
BASE COUNT         1589 a          979 c          779 g         2026 t
ORIGIN      
        1 ccgttgcggg taataccgta acactgaagg gcacgcatta aggcgtcatc ttaatgcttt
       61 cctgtaggac actgacggag attaccagtc tcccgagcct tacaggggcc tttctttttt
      121 ttgtattttc aattatcaca atttatcgtc caaaagtgca gaacgaaaat caattcatca
      181 caagcatatc agacaattaa atttaaaaca actaaaaata tacaaaaaag tgtaattgaa
      241 tattagcagc actgcctgaa aagaagtaat atcaggcttt tcagcttggg gaaataagtg
      301 aattacctct cttcatgaat tattataact caaaaaagca catatgcaaa cactagttcc
      361 ctattttttt gaatgaaaaa taactaaaat atctaacgat tatgacttta aaacctacgt
      421 ttgtgattgt tttcataaac ttttaatcta aaaactccgg aataatagct atttatttgt
      481 aacatttgta ataaaattaa aatcataaaa aagttctaat ttaaaattga tcatttttat
      541 agtgatcgct ttttatcaac tactctagcc aattttccgt actaactgtt ggtaattcgt
      601 taaatgtttc tctttgttcg tttgcaagct ttgccgcaat agctttttcg acaaaccaat
      661 tttttacata aacgaaaatt agattctgca aattgtttac tttttcagtt ttttgttttt
      721 gataagcttt cagcaaagtc atatataatc ctttgtcagc atcaattcca taagattcca
      781 gttcttcata aacaacaact gtttgctcta attcttcagc ttttttcttt gcattatgaa
      841 tagtttggac ggttttttga gcgtcttcgt aagtactaga aaatgttttt atcaagttta
      901 atacattttc tggtacaaat gtatttatac tgtcctcttt catgatgtcg acgaagttat
      961 ctagtaaaac ttgattttgt aactgatcag tttcagtgtc tattatgtgt ctattagtgt
     1021 ctatagaatt agaatagaat agattgtctg cgattttcgc aattacggtt gagtccttga
     1081 tatgactgga ttttgccgta tttgcgattt tcgcaattac ggtggtatca ttgatttctc
     1141 gcggttttgc cgtatttgcg attttcgcaa ttacggtggt atcagtattg tcttcctctt
     1201 ccatgatttt aaagacgtct tgattcgtta cttctggctt gcctaagtac aataaattag
     1261 gcatggattc aatcttaccg tcacgtttat ttactcttcc tctcttctgt ttcagaagac
     1321 ctgctttttc taattccttt ttgattttag atactttacc ttcgcggcat tgtaacaatt
     1381 tcattaattc ttcaactgtg aatatgaaaa atatattatc atcagaatct acccagttat
     1441 ttttgactga ataatcaaat cgatctttaa gtagcatata agcaattttt gcatcatttg
     1501 atagatccat gtatttttcg ttagtaaaaa ataccttagg catttgataa tatttttcac
     1561 gataaatttc ttttgatttg taataattaa aatcattcat agccaatcct ctttctgtag
     1621 gatagcgttt tgttcaaaac tatgttacaa tgaattaaac aaatatatct atccactata
     1681 tatttttcta ctgctgataa gtcgccaaac tttgttaaag cagtagtctt tttttatctt
     1741 tttttagttt accttaaaaa ataaattaaa gcaaaccgct ctatttcaag gtttaagctc
     1801 atagtaacta tatcacgata ttacaataat attatatcac gatatcgtaa tattacaata
     1861 tgaagataag cgacttatca gctttgatac tactgatttt tataaaaaaa atatcacgat
     1921 attatgatat cgtgatattg aatgtaaatt aaattttatt ttgtttcttt gctagtttat
     1981 atccttcaat caagtattct tcaggtatgg actttaatag cgcatcttct ataacgtctg
     2041 gtttactttt gtctgattca gcaatagtcg tccaatattt gagtgctgac attagaattg
     2101 gatttatacg aatgctatct gctgttctta cactacttcc ttgatctttt atgactttac
     2161 ttatattttt cttggttaat tcgatttctt ctacttcttt tgatttaaaa ttctttttat
     2221 ttttatttcc tagtaatcta cttactgcag cttgactgcg attatcttct gccatcaaaa
     2281 tcaccaactt cttttaaata ttcttctgta aatgctttat agtcatcttc agctgttgag
     2341 ggtttattaa aattgattag atctgatcca gcagcttgcg cttcgtcaac ggttattgtc
     2401 ctacgaatat aagttttgaa aatttttgca ccaatgactc tagaaatttc ggttgctgca
     2461 gacgtcattt ctttagaaat gttataacgc ttattatact tagtaaatag aactcctcgt
     2521 acttcaagct tgtcgttcgt atattctttt accgtttcaa tagtttcact taactttatt
     2581 agtcctttta atgagaaata cgcagcttca actggaatga taatttcatc agaaacagta
     2641 aatgcgttga ctgtaagcat tcctaaactt ggtggacaat caattataat ataatcatag
     2701 ttgtccttga ttggttgaag tactctttta agcctgtgtt cacttcctac ttgagtaaat
     2761 tccctctctg cagaagaaag ggctatgtcg gctggcaaaa catcaattcc tttatagtcc
     2821 agaatagcat ctgtaatgtc agcctcccct tttaatactt cataaattgt aggttggttt
     2881 gttccatcta tatcgaaacc tacattatct gtagcatttc cctgaggatc aatgtccact
     2941 aaaagtacat ttttaccgta aacaaatttc aaacatgctg ctaaattaac gctagtcgta
     3001 gttttagaaa ctccaccttt ttgattggct actgacaata ttcttgccat taaaatatcc
     3061 cccattcatc ttatattata atataattat atcataatat tgtgatattg caatatcgta
     3121 tatgcattat attgtaatat tgtgtgatta ttttgtttta aagatatata tcggttcgga
     3181 tataaagatt attcatatgt tattcgaaat taaaataatc actttttatt actttaaaat
     3241 tttttattaa tgataagaaa tatgttttgc gagcagcaac tagaataatt taaacattac
     3301 taaactgtct atattgttac taataattcc cttcttatag tgcattttct tagaaaaaaa
     3361 caatattacg atatcatgat attaagatat cgtaatatta tacaattgtg attgatatta
     3421 tatatgtttt tttatttttt tatcatgttt aatcttgaag gaatcatatt ctttttctaa
     3481 agacaggagc ttttcataaa catcagacat gtttaaacta gaggccaggc ttaaatcata
     3541 aacgaatgaa gcaggtaatg attttattct acgttgtcgg gttatccagc ttgcaaccgt
     3601 tccttgtggg taggcatata actcacaaaa ttgttctaca gtgattccta aattcttgat
     3661 aatgtaaaca ttgatcgggt gcgagtaaac aaatgtattg atagacatgg tgcgtaagcc
     3721 tactttcttt tattattgcg tttgcaataa tagtttaaca cacgcattat tctagtatct
     3781 actcctattt gtaaaaacaa aaaaggattc atagagataa ctctatagaa tccgtaaaat
     3841 accttatata tataacggga agatataaaa cctaaaatta tgataacctg ctgcagcttc
     3901 tacatagaaa gagaagcgct caacaccaac ctcatttatc aaacgttggc cataacccca
     3961 aatctcttct gatagctgat caaaggttat atactttagc tcatattgct ggataatgtt
     4021 cattctaatc gactccattt attatcctgg taaaattcga tatgacgtat ttcgtcgtag
     4081 tgaattttgt cttttccaac gtagatcccc agctcatcac tacctaagat cttgccaatg
     4141 atatcttctg gatagtatcc ttcagcgttt cgttcttctt tttgaataga tactgatcta
     4201 ttttttatga ctgcagcgtt caatacttcc gcaatctctt cagtcgtcat ttgttctttt
     4261 gctaaatttt gactggttcg ttgttcattg tcggcatcaa tttcttcagt atgatcagat
     4321 aaataaaacc ctgcccactt taaacgtcca cgatcattgt aatcaatcat ttttaattcc
     4381 cccatttccg cccgcatgac caccaacaag tgtgcttcta gtaatgcttc tagcgccttc
     4441 taacattgaa gaagcatgta caatcgacgt aaaaccatat ttttgccgaa tcttgtcaat
     4501 aattttatct aaattttctt cgtcaatttg cttttgtgga tcactaaata aatccagctg
     4561 aagcgaatcg gtgtacacaa gcttgctata ggttattcca acatgcctta cttcttgtcc
     4621 ttcataatac ttatcaaaca agaaaagaca ataattctgc aattcctttg tattatctgt
     4681 gatagggatc ttcatctggt gtgaaaaccc tggtctcgtt tctaagatcg acgtgccaat
     4741 gtgtaaatgg acacaacctg ttttacagtt atgctgccta atgcgtatcg ctacttgttc
     4801 cgccatttct tttaccacca gctcgatttc atttcttcta gtataatctc gtggtaacac
     4861 ttgtgaattt ccatatgatt tttcctttgt cggttctggt gggattgcta tatctgttcg
     4921 atcgatccca ttagcatgga agtacagctg taagccaatc acacctagtc tattcttaat
     4981 cgtataagga ttccagttag caagatctct aatgctcata atccccatct gattgagtct
     5041 ttttttcatt cgagagccaa ttccccaaaa gtccgtcatt tcaggaatat tccatacttt
     5101 ttcaggaacg ttttcgtacg tccattccgc aacaaatcct tcattatgct ttgcttcgtt
     5161 gtctaaggct agtttagcca aaaggggatt atctccgaca cctactgtag caatcaatcc
     5221 tagctcttct ttaatattct cctggatcat ttgagccagc ttctttctcc gttgacttcg
     5281 tgttccttca gtcgtaaaaa gattcagtga tcgggtcact tttaagattg attcatcgat
     5341 cgagtaaatc agtagatctt catcagccac ata
//
LOCUS       DANKEF010000112         5339 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.114, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000112 DANKEF010000000
VERSION     DANKEF010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.114"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(19..414)
                     /locus_tag="PWZ48_002766"
     CDS             complement(19..414)
                     /locus_tag="PWZ48_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262373.1"
                     /translation="MKNKGQIWYIGYIVSLFLIVTILVTDFPKMVDIGLAILFSAVFG
                     VSHVQILHDKMIKNDADYKINVMDERNIIIKEKAGNITNMFNTILLGVATVIFICLDY
                     IIPAIIVGSIIAIQPIILIIISSVLEKKM"
     gene            complement(404..616)
                     /locus_tag="PWZ48_002767"
     CDS             complement(404..616)
                     /locus_tag="PWZ48_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2262374.1"
                     /translation="MKNRLEELRKQRGIKQEDLASALEVSRQTIGSLENGRYNPSIIL
                     AFKIARYFQMSIEEIFIYEEESEDEK"
     gene            1688..2290
                     /locus_tag="PWZ48_002768"
     CDS             1688..2290
                     /locus_tag="PWZ48_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262375.1"
                     /translation="MEDIILRDRLTDPLKNSLANVIYDILREADKVYSNYNGKVSWVI
                     YSFLIKEFFKEQINNSYELGYHFTVYYEDIIKRLEDLEWYAYYDLLEVFASAKILDKK
                     KREEVNKILESENSGYRLSESNEFIPITDDISIKSINAVSESPFNYAKNHIHKSLTYI
                     SEKENQDYNTVVREAISAVESCLIEFSGLPANKKILWELL"
     gene            2645..2881
                     /locus_tag="PWZ48_002769"
     CDS             2645..2881
                     /locus_tag="PWZ48_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HDL2262376.1"
                     /translation="MNIKKRKIREVGNSIVVTLSKESLLQKGLKPGDTIFIDEDKMME
                     AIVKEESKIDLEIDMYVNQALSEYDAAFKELVEK"
     gene            2878..3291
                     /locus_tag="PWZ48_002770"
     CDS             2878..3291
                     /locus_tag="PWZ48_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HDL2262377.1"
                     /translation="MIYLSEKDIVKLNVIQIKKYSPRETIGVIDKNALSMAVNQPKQH
                     IFGKDLYPDKFSKAAVLLINLIKKHPFHNANKRTAFLAAYVFLKLNGYSLKMKNQEVV
                     EFVVEIATYQGDFDDLKEATTDILKKNSTKTDSKK"
     gene            3810..4760
                     /locus_tag="PWZ48_002771"
     CDS             3810..4760
                     /locus_tag="PWZ48_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HDL2262378.1"
                     /translation="MERNSENKMTGRNPKVFISYSWENKENKEWARELADKLLENGID
                     AYIDQYDLSLGDRLPQFMEQQISDSDYVLIICTPEYKKKANNRTSGVGYEGHIISAEL
                     LSGKNERKFIPIIKEGRASEVLPTFLAGKLGIDLSDSQHYDENLDDLITTIFGVKRKP
                     ALGDIPRHIKGSPLLPIEHNENKDIEILGIITDKVTVPKMDGSRGSALYKIPFRLNRV
                     PSQLWKELFVRNWNSPPRYTTMHRPGIASVVDDEILLNGTTIEEVREYHRETLKLCIE
                     KSNTQEKKILLEEEQKKERENERVRSHKESVQKLADELEF"
     gene            complement(4757..>5339)
                     /locus_tag="PWZ48_002772"
     CDS             complement(4757..>5339)
                     /locus_tag="PWZ48_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262379.1"
                     /translation="SIDQNEQRQIEEMRKFGAEKIFIEKQSGATITHRPIFQEVLDFV
                     RDQDIFIVEAIDRLGRNYDEIIDSVNYLKKKNVRLIITNLPIMAEAIGNPLLDKFIKD
                     LIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSANTKDPQRRLVYRSIV
                     QDQENGVAISKIATDYNVTRQTVYRIKKELTIN"
BASE COUNT         1908 a          760 c          902 g         1769 t
ORIGIN      
        1 ttgtatgaac ctttatttct acattttttt ctcaagcaca ctactgataa tgattaaaat
       61 aatcggctgg attgcaatga tacttccgac aataatagct ggaatgatgt agtctaaaca
      121 aataaatata actgttgcca cccctaacag tattgtgtta aacatatttg ttatatttcc
      181 cgctttttct ttgattatga tattccgttc gtccataaca ttgattttgt agtctgcatc
      241 atttttaatc attttatcat gcaaaatttg aacatgagaa acgccaaaaa cggcactgaa
      301 taaaatagca agtccaatat ctaccatttt aggaaaatct gtaaccaaaa ttgtcacaat
      361 caaaaacaac gaaacaatat aaccgatata ccaaatctga cccttatttt tcatcttcac
      421 tttcctcctc atagataaaa atttcttcaa tgctcatttg aaagtacctt gcaattttaa
      481 atgccaatat aatagaggga ttataacgtc cattttctaa agaacctatt gtttgacgag
      541 atacttcaag agcagaagct aaatcttctt gtttaattcc tctttgtttt cgtaattctt
      601 ccaaacgatt tttcacaaca tgctcctttc taaaaaatgg aaagtttact ttcctttcgt
      661 gcttttataa tactcctgcg agcacaaaat gtcaaggaag ctttccatat aaattttgat
      721 tttaatgttt tagttactgt ttttatgaca catgaacaca cttattctta tttcctctta
      781 tctgctatca acagaaaaga aaaagccctg tcaagagccg attagatcaa gccagtcgag
      841 aaacgatgtt agaccaaatt gattttgggt aatgaatgtt tagaacttag tagtcatcca
      901 aaaacgacta ctttttttgt tgaatcgtcc aaggacgatt cgatgctttt gacttgtaag
      961 aacaggattg aatgaaacca tactgttcgc atatttttag tggatgaaca aacaaaatac
     1021 gagagatttt ttgttcgttc atccatggtt ttagaaaaaa gagggacgat tttggaagaa
     1081 gaaaatcgtc tctttttttt cttctttttg tatgacaaaa agaaagatct tttgcccatt
     1141 ttatttttat aaaatgggta ggtggcgttc gcgtaaagca aatcgataca atccaatggt
     1201 tttggcgaat cagatttacc attctaggct tgtttaatac agacatcgat tgttattttg
     1261 agaatttata tccttattgg caatgaaatg gatattctcc ataccaatag aaatcttcaa
     1321 gataattttc ggaaatatat aagagttaat caattatgat catatagtag gtaaaaatta
     1381 ggacagcctt caattatatg ggaaaccaat ataatcaagt aattattcgg gtaacacaaa
     1441 tactagagta ctcatttctt ataactgttt tacacctatt agggatcagt attattaata
     1501 gatctagata aattaatacg taattatata ataatttatg tttgtgccat taaacaatct
     1561 taacagatat ttcattttat ttatgtaaaa caacacgaat aattattgct tcgtaaagtt
     1621 atatgagtat aagttaattt actggaggta gtgcaatgct tttttctgaa agatttggtt
     1681 ttaaaaaatg gaggatatca tactgagaga tagattgaca gaccctctta aaaatagttt
     1741 agcaaatgtg atttatgata tattacgaga ggctgataaa gtttatagta attataatgg
     1801 aaaagtctca tgggtaatat attctttttt aattaaagag ttttttaaag aacagataaa
     1861 taatagctat gagttgggtt atcattttac tgtttattat gaagatatta tcaagaggtt
     1921 agaagacttg gagtggtatg cttattatga tttattagag gtttttgcaa gtgctaaaat
     1981 cttggataaa aagaaaagag aggaagtaaa taaaattctg gagtcggaaa attctggtta
     2041 cagattaagc gaaagcaatg aatttattcc aatcacagat gatatttcta ttaagtctat
     2101 aaatgctgta tctgaatcac catttaatta cgcaaaaaat catatccata aatctcttac
     2161 atatataagc gagaaggaaa atcaagatta taatactgta gtgagagaag caattagtgc
     2221 tgtcgaaagt tgtcttatcg aattttcagg attaccagct aataaaaaaa tactttggga
     2281 actgctataa aagcaattaa agacaaatcg aataacaatg tcgatcttac atttttaaaa
     2341 ccatttgaga caatgtatgg tttggcgagt aataatggaa tacgacatgc agaaaatgaa
     2401 aaaacaattg tctcagattt acctgatgca gttttggtgt taacaacatg ttcagccgta
     2461 attaattatt tgggagtaaa aatagcatat gactccaata aatcatgaaa agttaaaatt
     2521 catccaagag ctacattata actattaatt tccaactctt taaaagttta gtaattgaag
     2581 gaagtaataa gggtgattga acaatacgga tatacggtgt atacttatcg taggagttga
     2641 tgatatgaat attaaaaaac gcaaaatcag agaagtaggt aattcaatag ttgtaacttt
     2701 gtcaaaagag agtttattgc aaaaaggttt gaaacctgga gatactattt ttattgatga
     2761 agataaaatg atggaagcta ttgtaaaaga ggaaagcaag atagatcttg aaattgatat
     2821 gtatgttaac caagctttat ctgagtatga tgcagctttc aaggaattag ttgaaaaatg
     2881 atctatctaa gtgaaaaaga tattgtaaaa ttgaatgtta ttcaaataaa gaaatattca
     2941 ccgcgagaaa caatcggagt cattgacaaa aatgcgctga gtatggctgt aaatcaacct
     3001 aaacagcata tttttggaaa agatctgtat cctgataaat ttagcaaagc agctgttctg
     3061 ctaataaatc tgatcaaaaa gcatccattt cataatgcta acaaacgtac agcattttta
     3121 gccgcgtatg tttttctcaa gctaaatggc tattcactaa aaatgaaaaa tcaggaagta
     3181 gtagaatttg tagttgaaat tgctacgtat cagggtgatt ttgatgactt aaaagaggca
     3241 acaacagata tactaaaaaa gaattcaaca aagaccgatt caaaaaagta gaagcaaact
     3301 agggattcag ttaaatctga agatttagtt tgttctttat tttagtttac ataagataca
     3361 ttatgcgaag ttcattattt taaaagctta aataataaac ttttttataa taccaattta
     3421 tttatgtaaa ccaatgacct taatcgctta agcaagcatg ttatgtaata ctctactctc
     3481 tccaaaacta atgactatct attttcaaag aagctagaat gattatctga tcaaatgata
     3541 atttatattt aattcctggt ctactgctct tcttttaatc aattaatgtc taacgtactg
     3601 tacacgataa agtaggaaga cttattttca acttcggatt cattttttta attcggtgaa
     3661 aatcactaaa ttaagagttt atccaagttc attttagctg agaatacgct tgaaaataat
     3721 tttgatgaat tttcctgaat taaaatttat taacttgttg agtgatataa ttgtaatgta
     3781 ttaccaaata gttagcactt agattagtga tggaaaggaa tagtgagaac aagatgactg
     3841 gtagaaatcc caaagtattt atatcatatt catgggagaa taaggaaaat aaagaatggg
     3901 ctagagaact agcagacaaa ttacttgaaa acggtattga tgcatatata gatcagtatg
     3961 atttaagttt aggtgataga ttacctcagt ttatggagca gcaaatatcc gattcagatt
     4021 atgttctaat tatatgtaca ccagagtata aaaaaaaggc aaataatcgt acaagtggtg
     4081 ttgggtatga aggtcatatt atctcagcag aattgctttc tggaaaaaat gaacgtaaat
     4141 ttatcccaat aattaaagaa ggacgtgcaa gcgaagtact tcctactttt cttgctggaa
     4201 agcttggaat tgatttatca gatagccagc attatgatga aaacttagat gatttaatta
     4261 caactatatt tggtgttaag agaaaaccag cattaggtga tatacctagg catattaaag
     4321 gcagtccttt actacctata gaacataatg aaaataaaga tattgaaatc ttaggaatta
     4381 tcactgataa agtaactgta cctaaaatgg atggctcaag gggttctgca ttatataaaa
     4441 ttccatttag attaaataga gtaccaagtc agctttggaa agagttgttt gttagaaatt
     4501 ggaattcacc accacgatat acaacaatgc atagaccagg aattgcttct gttgtagacg
     4561 atgaaatttt acttaatgga acaactattg aggaagtccg ggaatatcat cgggaaactt
     4621 taaaactttg cattgaaaaa agtaatactc aagaaaaaaa aatattgcta gaggaggaac
     4681 aaaaaaaaga gagagagaat gagcgagtca gatcacataa agaaagtgtt cagaaattag
     4741 cagatgaatt agagttctag ttgatagtca gttctttctt aattcgatac acagtttgtc
     4801 tagttacatt atagtctgtt gctatttttg aaatagcaac accattctcc tggtcctgaa
     4861 cgatgcttct ataaacaagg cgacgttgag gatcttttgt gttcgcactg tataacttag
     4921 gacgtccttt atagacgccg tttgctttag caatctttat cccttgtgcc tggcgtcgtt
     4981 ttgattcagt acgctcttgt tcagcaatca tggccaagat ttgaatgatt aggtctttga
     5041 taaatttatc caggagagga tttccgattg cttcagccat gatcggtaaa ttagtgataa
     5101 taagtcgcac atttttcttc tttagataat ttactgaatc gataatctcg tcataattac
     5161 gtccaagtcg atcgatagcc tctacaataa aaatatcttg gtctctaaca aaatctagaa
     5221 cttcctgaaa aatcggtcga tgagtaatag tcgctccaga ttgtttctcg atgaaaattt
     5281 tttcagcacc aaattttctc atttcctcga tttgacgttg ttcattttga tcgatcgat
//
LOCUS       DANKEF010000113         5275 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000113 DANKEF010000000
VERSION     DANKEF010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.115"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            439..537
                     /locus_tag="PWZ48_002773"
     CDS             439..537
                     /locus_tag="PWZ48_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2262380.1"
                     /translation="MYEKFLTLVVAPLFVGLVIELVSHWLDEKDDN"
     gene            complement(691..1188)
                     /locus_tag="PWZ48_002774"
     CDS             complement(691..1188)
                     /locus_tag="PWZ48_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262381.1"
                     /translation="MKLFFEKMGWHILPVVIILVGSYSLFCIFRSILEKFEEPKLKRV
                     VFALCFAFLALFVSVGISSYRPDVALSVRPKISKIEQLEAKAEAKQKKIDKISDQLEQ
                     ALKIASQEKLKTFNPKDYPSEVITADWVQKNRDVIATLPDKELKDRYTKRFDDVESYF
                     ELNSQ"
     gene            complement(1216..1671)
                     /locus_tag="PWZ48_002775"
     CDS             complement(1216..1671)
                     /locus_tag="PWZ48_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262382.1"
                     /translation="MPLDDFASKQELQKVKQELAEIKQQNQEVIETLKSLDNSVKQGY
                     IKVRGGEDIPKMSELKAEMNRYATYVQKRTAEIDQKLDSYGNISKSVSDELSRQVRPQ
                     VQKFLANDDRATAEELKQNRIANLMSVLASVGLFIGGTAILIVSVLKLL"
     gene            complement(1679..2665)
                     /locus_tag="PWZ48_002776"
     CDS             complement(1679..2665)
                     /locus_tag="PWZ48_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2262383.1"
                     /translation="MGATLALKGGKRGASALIGYVNRHGKLDRDRIYTHKLSAENKLA
                     QTQLEATRRTFAKTGGIQAFHWVQSFDNSFDKNNPADVQKAHEIGAKLVDEIAKSYPN
                     HEIYMGTHTDSESGYIHNHIVFASIDTETGKKFHSDTEVGYHLQDLNDKVLLSFGIEQ
                     PKEKFKQVDFEMHAEGKMSNREKMKHMILGAQDMSTDFDSFKKNLQEAYAVEVYEYNK
                     GKRIGFKWVDDEGKDFTIGGRKLGSDFERPAIERTLEFSQQKQEKEVEKPLETKPKVT
                     NDDFSISGLKNLANDIRQQEERKKKEELQRQKEIERQQRSRPFYHDRGGMER"
     gene            complement(2650..3006)
                     /gene="mobC"
                     /locus_tag="PWZ48_002777"
     CDS             complement(2650..3006)
                     /gene="mobC"
                     /locus_tag="PWZ48_002777"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid mobilization relaxosome protein MobC"
                     /protein_id="HDL2262384.1"
                     /translation="MSEQKLNAENRKDSRQIKFRVSEDEYQYLTAIADQTGMSVPQLC
                     KSVALGMKYRAPNVDREGAIQIAKELRKYGGNLNQMARFINMNKGQMPSSSDLALMNQ
                     QLSQIEKGLIDLWEQL"
     gene            complement(3562..3927)
                     /locus_tag="PWZ48_002778"
     CDS             complement(3562..3927)
                     /locus_tag="PWZ48_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262385.1"
                     /translation="MAKMDIEDLEKKLAEARHDLSVKIGTNMVEKQGFKSYSAYNTFM
                     RNASKALKSSAGSAPAPAAAKPVATSAEKLPSELKAEAVQFQKQLEKWMEDNWGNKLD
                     YKTFYPELLEFFQKLNSLE"
     gene            complement(3936..4097)
                     /locus_tag="PWZ48_002779"
     CDS             complement(3936..4097)
                     /locus_tag="PWZ48_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262386.1"
                     /translation="MTAKTVTIDVEQLEKLLERHSDAIIEYTGGLSEETINQFDLLLT
                     EIIELDSKE"
     gene            complement(4087..4236)
                     /locus_tag="PWZ48_002780"
     CDS             complement(4087..4236)
                     /locus_tag="PWZ48_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262387.1"
                     /translation="MKKRLRKKMAKKNSSLDQAMKELMNYAKGERDGFLIHSYDEKGE
                     QKNDG"
     gene            complement(4250..4999)
                     /locus_tag="PWZ48_002781"
     CDS             complement(4250..4999)
                     /locus_tag="PWZ48_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262388.1"
                     /translation="MGQPNKKADIALKDLLSRQDYLVVQGNDLAKAFGGLKSFEQRVL
                     DYCFSFVTKDSKLDDEYEVTALEIIKHFGLNTSGDSYRRIAEAFKKLNENTALYLRVE
                     RPDGTQGLRMTQLFSWIDFYADGLVRFKFSADSAPYVLDLKEKFYSFRLGELSRINGK
                     YALIMLKLWESYRRGKEKHTTITGDLEEWESWFLGKDKRLPAGQFFQKVITRAIEELS
                     DKMSVSFFVTTLKRGRKVVGYEITINDTSKE"
BASE COUNT         1373 a         1195 c          976 g         1731 t
ORIGIN      
        1 aaaaaaaaaa aaatcccgcc caaaccagtt gtctattttt ttccaacagg ttcgcgcatg
       61 aaataaactg cttctcacat catcgacaat ttatcccctt tacctcgttc aatcttacag
      121 ccacaaggca taaaggagtg atcgctacgc tctcacattt tgcttacagc agtcgcctgc
      181 gctccttcgt taaggtcaaa accgttgttc aaggagcggt tgggataggc gtaaaagctt
      241 gttgtagcaa gtttttagac tgatttagat taaatcgtta gtcttgttat acagcgcctt
      301 tacaggcggt cagggcaagc cctaacgacc caaaagcccc atctggtaga ccatcttttt
      361 tttgcaggga gtgaatgcta tattaatctt ggcactaaag ccaactaacg cttacacagg
      421 ttgggaggtg tattccgtat gtacgaaaaa tttctaacgt tggttgtcgc accgctattt
      481 gtcgggctag tcattgagct agtttctcat tggttggacg agaaagacga caactaaggc
      541 ttagtgcctt ccaactcaca cgcctagtcg cagggcgaaa aaaaagccag acaccgtcgc
      601 atggtgtctg gcttttgtgc attccgcact cgaaaaaatt ctaacgtttt cgcatggtta
      661 ttatagcatg gacgttggca aatctcaatt tcactgacta ttcagctcaa aataggattc
      721 tacatcgtca aatcgctttg tgtagcggtc tttcagctct ttatcgggca aagtagcgat
      781 cacgtcacga tttttttgta cccaatccgc cgtaataact tcgcttggat agtcttttgg
      841 gttaaaggtt ttcagctttt cttgactggc aattttcaat gcctgctcca gttggtccga
      901 aattttatca atttttttct gttttgcttc ggctttcgct tctagttgct ctattttgct
      961 gattttaggg cgtacagaca gcgcaacgtc cggacggtat gaagaaatgc cgaccgacac
     1021 aaaaagcgct agaaacgcaa aacagagggc aaaaacgaca cgtttcagct ttggttcctc
     1081 aaatttttct aagatcgagc gaaaaatgca gaataaacta tacgaaccga ccaaaataat
     1141 cacgacagga agaatatgcc accccatttt ttcaaagaac agtttcattt gcgttttaag
     1201 ccccctctcg ttcgattaca ggagctttag gactgaaaca ataagaatag cagtgccacc
     1261 gataaaaagc cccacagagg ctaatacgga cattaaattc gctattcgat tttgtttcag
     1321 ctcttcggca gtcgctcgat catcgttggc taaaaatttt tggacttgtg ggcgcacttg
     1381 ccggctcaat tcgtcagata cagatttgct gatattaccg tagctatcaa gcttttggtc
     1441 gatttcagcg gttctttttt gaacgtaggt ggcgtagcgg ttcatttcag ctttcagctc
     1501 gctcattttg ggaatatctt cgccacctcg caccttgata tagccttgtt ttacgctgtt
     1561 atcaaggctt ttcagcgttt caatgacttc ttgattttgt tgcttgattt ccgctagttc
     1621 ctgcttcact ttttgaagct cctgcttact tgcaaaatca tctaacggca tatcgtcact
     1681 acctttccat gccaccacga tcatgataga acgggcgaga acgttgttga cgctcgattt
     1741 ctttttgtcg ttgcagctcc tcttttttct tgcgttcttc ttgctgacga atatcattag
     1801 caagattttt caaaccgctg atagaaaagt catcattggt gactttaggc ttggtttcaa
     1861 gcggtttttc gacttctttt tcttgttttt gttgagaaaa ttcaagtgtt cgttcgatcg
     1921 cagggcgctc aaaatcagaa cctaattttc gtccgccgat tgtgaaatct ttgccctcat
     1981 catcgaccca tttgaagcca atccttttgc ctttattgta ctcataaacc tcgacagcat
     2041 aggcttcttg aagatttttt ttgaaagaat caaaatcggt actcatatct tgtgcgccca
     2101 ggatcatatg cttcattttt tctcgattac tcattttgcc ctcggcgtgc atttcaaaat
     2161 caacttgttt gaatttttct ttcggctgtt caatgccaaa actcaataaa accttatcgt
     2221 ttaggtcttg taggtggtag ccgacctcgg tatcagagtg aaatttttta ccggtttcag
     2281 tgtcgatact ggcgaaaaca atgtgattat gaatatagcc ggattctgaa tcggtatgag
     2341 tgcccatgta gatttcatga ttgggatagc tttttgcgat ttcatcaacg agtttagcac
     2401 cgatctcatg cgctttttgc acgtccgccg gattgttttt gtcaaacgaa ttatcaaacg
     2461 attgaaccca atgaaaggct tgaataccgc ctgttttggc gaaagtccgt ctagtcgctt
     2521 ctagctgagt ttgagccaat ttattctcag ctgacagttt gtgcgtataa atgcggtcac
     2581 gatcaagctt tccatgccga tttacatagc caatcagcgc agaagcccca cgtttaccgc
     2641 cttttaaagc taaagttgct cccataagtc aatcaacccc ttttcgattt gcgaaagttg
     2701 ctggttcatc aaggctaaat ctgaacttga aggcatttgc cccttattca tgttaataaa
     2761 gcgagccatc tgattgagat ttccgccata tttgcgcaac tctttggcaa tttgtatcgc
     2821 cccctcccga tcaacgttcg gcgctcgata cttcatgcca agtgccacgc tcttgcagag
     2881 ctgtggcaca ctcataccag tttgatccgc aatggcggtc aaatactggt actcgtcttc
     2941 gctcactcga aatttaattt gccgactatc ttttcgattt tcggcattca atttctgctc
     3001 gctcatctca tcactcctca aataaaaatg ctccctattc ggtcgctttt ttcttctcgt
     3061 cattcttcgt ggtcgatatt caaagctaca aaaacgctcc ctattcagtc gccttttttc
     3121 cgcaatgaaa tctccctcgg ttatgccact acaaaatgaa agttccctac tcggtcactt
     3181 ccctttttcc gctctataac cgaatgattt attaacctca tcatactccc tattcggtcg
     3241 cataacaagg ttaataaatc atataatacg caagcaccgc atttggtacc caaatacatc
     3301 gcttgtcgag gtggcgttgc cccctacgaa ctcccccaat cacgcaacaa aaagtagcct
     3361 gttcggctat ttttattacg tgatttcgcc tcagaaaaag tagtctgttc gactattttt
     3421 ctactggctc atgcggtcaa acttgcacgc aagttgacct tatttttttg tctgcccgct
     3481 gttcgcaggc aaacaaaaaa gcaagccaaa aatcggcttg ctcgttcttc aaaaattgat
     3541 cgcatggact agctcgttcc gctattccaa gctgttcaat ttttggaaga actctagcaa
     3601 ctcaggataa aatgttttgt aatccaactt gtttccccag ttgtcttcca tccatttttc
     3661 gagttgcttt tgaaattgca ccgcttcggc tttcagctcg ctaggcaatt tttcggcaga
     3721 agtggccacc ggctttgcag ctgctggcgc aggtgcagat ccggcggacg acttcaatgc
     3781 ctttgacgca ttccgcatga aagtattgta ggcggaatag ctcttgaacc cttgtttttc
     3841 aaccatattt gtaccgattt tgactgaaag atcatgccga gcttcagcta attttttttc
     3901 caaatcttcg atatccattt ttgccatgcg taaactcact ccttagaatc tagttcgata
     3961 atttccgtta gaagaagatc aaattggttg atcgtttctt ctgaaagtcc accggtgtat
     4021 tcaataatag cgtcactatg cctttctaac agcttttcaa gctgttcaac gtcaatcgta
     4081 actgtcttag ccgtcatttt tttgttctcc tttttcgtca taactatgaa tcaaaaagcc
     4141 gtcacgttcg ccttttgcat agttcattag ttctttcata gcttgatcta gcgaagaatt
     4201 tttctttgcc atctttttac gcagtctttt tttcaactaa atcactcctc tattctttgc
     4261 tggtgtcatt gatcgtgatt tcatatccaa cgacttttcg cccacgcttt aaggttgtga
     4321 cgaaaaatga aactgacatc ttatcagata attcttcaat agcacgagta attacttttt
     4381 gaaaaaattg acctgcagga aggcgtttgt ctttaccaag aaaccaagat tcccattctt
     4441 ccaaatcacc tgtaatcgtt gtgtgttttt cttttcctct acggtaggat tcccagagtt
     4501 ttaacattat gagagcatat ttcccattga tccgagataa ttcacctaaa cgaaagctat
     4561 aaaacttttc tttgagatca agaacgtatg gagctgaatc agcagaaaat ttgaatctta
     4621 ctaacccgtc agcataaaaa tcaatccaag aaaataattg cgtcattctg agaccttgag
     4681 taccatcggg gcgctcgaca cgaagataaa gcgcagtatt ttcattaagc tttttgaaag
     4741 cttcagctat tcgacggtaa gaatctccag aggtgttcaa cccaaaatgt ttgataattt
     4801 ctaatgcggt tacctcgtat tcgtcatcta gttttgaatc tttagttaca aagctaaaac
     4861 agtagtctaa aactcgttgc tcaaacgatt taagccctcc aaatgcctta gctaagtcgt
     4921 ttccttgcac tactaaataa tcttgacggc ttagtaagtc ttttaatgca atatcagctt
     4981 ttttatttgg ttgtcccaaa ctgaaaatcc tccttttgta ctttttttaa aataatacca
     5041 tttattcagt ttttttgcaa tatttgaatt ttcaacgtgg aaaatagtat ttttgacgtg
     5101 gaaaatagta tttttgacgt ggaaaatagt attttcaacg tggaaaatag tatttatagg
     5161 cgctacaaac gctggtataa agccattcct tttcccctaa agtattttaa agtatttaaa
     5221 agtaatctat aaagtattct ctttaagcgc gcgggtgaga aaaaatttta aattc
//
LOCUS       DANKEF010000114         5268 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000114 DANKEF010000000
VERSION     DANKEF010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5268
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.116"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(30..434)
                     /locus_tag="PWZ48_002782"
     CDS             complement(30..434)
                     /locus_tag="PWZ48_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262389.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            complement(427..828)
                     /locus_tag="PWZ48_002783"
     CDS             complement(427..828)
                     /locus_tag="PWZ48_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262390.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            complement(818..1171)
                     /locus_tag="PWZ48_002784"
     CDS             complement(818..1171)
                     /locus_tag="PWZ48_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HDL2262391.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            complement(1161..1472)
                     /locus_tag="PWZ48_002785"
     CDS             complement(1161..1472)
                     /locus_tag="PWZ48_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262392.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            complement(1469..2344)
                     /locus_tag="PWZ48_002786"
     CDS             complement(1469..2344)
                     /locus_tag="PWZ48_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262393.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            complement(2354..3514)
                     /locus_tag="PWZ48_002787"
     CDS             complement(2354..3514)
                     /locus_tag="PWZ48_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HDL2262394.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            complement(3514..4200)
                     /locus_tag="PWZ48_002788"
     CDS             complement(3514..4200)
                     /locus_tag="PWZ48_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HDL2262395.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            complement(4163..>5268)
                     /locus_tag="PWZ48_002789"
     CDS             complement(4163..>5268)
                     /locus_tag="PWZ48_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDL2262396.1"
                     /translation="LQVDFVYRKFAIETCIDLIANAMSKAEFKSYEDGKNKKNDLYYR
                     LNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSPSNNSSEIFIADSWDVTEYALKDDVF
                     SQVQINNIVLDREFLESDVIYIKYADQQIRQLVDAYYQAYGKLISSAMNVYKRSNARR
                     YVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEADNAGAVFQLQNEYTLEDFSGNFQSN
                     SRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGLSDQVDAFLMFEIIPIAELIQDAFNA
                     SLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAIDVGIRNGVFTINEGRERVGNDRSDK
                     AMADEIFITKNNQQVSKGGEANDDNENISSSKE"
BASE COUNT         1505 a         1089 c          779 g         1895 t
ORIGIN      
        1 agtttttgtt ttagccattg ttgttccccc tatttattta atttatcgaa ttgacgaatc
       61 agaagttctg taattggatc aagtgcaaga cctaatcctc ttctcataaa ttcgtccggc
      121 tgatttcttt tagaagtacc tatccccaaa tcaggatatt ttaaatactc aaattttctt
      181 gtaggtctaa tgatgaaacc caaattaatg tattgagtct taagtggacg actatttttt
      241 gcgtgttggt gccctcttct taaatctgct tcagaaacag gaattttttc tgtaatccta
      301 tccactgcaa tagccgaacc ttttgatttc aatgcttcgt taatcagtcg ttcgctctcg
      361 cttgaatagc gttccatccg cacaagaagt tcatcatgtc catttatttt tagctcccaa
      421 ctatttttag ccatgacaat cactcttcaa taatcgtcta aacgtaaata ccaattgatc
      481 gatatagcga tcttggttct ctagttttaa atgattggga tccattctct gaaaacgaat
      541 cgaacgattt tgaatcaatg aaataatatc tagtgagtct cctgttaaat cttctctatt
      601 ttctgaatag aaagttagat atagattttg acccacgcta tattttggct cagtgatcat
      661 ttctatttct cctgtttcga gaatgaagta attaaaatca tcaggtagct catcctcgcc
      721 tacggagtct tgaaagagtt tgaggccaaa atgctcttct aaggaagttt tgatagcaga
      781 aatttgctta tttaaacgtt ctttttcttt agaattatca atcaccatat tcacccacac
      841 tttcaagata aaaatagata taaaaattat cgtaatcggc atagataacg ttgtaacgca
      901 tactatcgat tacgataaaa tattgatctt tattaaattt cttggcgatt ggatgaaatg
      961 gagtctttac tttcttagtt aatttcgatc ccatcgcatc catagctgtt atatcactat
     1021 ctctcatgga aagatttcta aattttaaag aagtgatttc tgtatcttct acaccaatct
     1081 tttttcctag ttcatttctt ttggtagttt gcgtcaaaat ctttaaccaa ccatcgttga
     1141 atgtttcttc gagtctacga ttattcgcca ttcacatcac ctgcaatata ttcttgtagc
     1201 gcataatgtt gaatgaaacc taataactca ctagcgaaat tttgttcaaa ctcatctaaa
     1261 gcacgattcc agtcgtatct acatctttcg attagcaatc cgtattctaa gctttcagga
     1321 gaaaaagaaa gtgttgtact cactttactt tgaagataaa cagcattttt agctatcatc
     1381 tttttaattg actcatcttc ttcgttccag gtaacgtaaa tattatcctt cacagctatt
     1441 agcaattctt cagtcacttg ttcaggcgtc atctaaccac cgccttaatt gctttaacat
     1501 atgcgtaaga gcattttttc ttgtttacaa atgataaatc ttcatcaaaa ggcgtagaag
     1561 tcacgtatct ccctttgaaa aataaatctt catcgtttgt tgttactcca gcattgtgta
     1621 agatttttac ttctttaact ttttctattg gatcagtagc aaaacaaaag tctaattcct
     1681 cgtgaacttt aggaccaata ttgaaataca tcatgttcca aagctgtgcc cacatctcgg
     1741 ctgtccagat ttgtatattt gttttttgcc ctctaaggta gcgatatagc cgattagaat
     1801 ccagataaac ctttttccaa taattcgctt taggacggtt aataacccac tgtgcgcctc
     1861 ctgaattagt gtttatagtt tccaaagatt ctactgtaac atttacaatg tttgccatat
     1921 cttttagaat attttctccg ttttcacagc ttctaatata atcaagactt agataactac
     1981 agcagtcgct acaataccaa acatcatctt tagaaggcaa tttgcgcaaa ttaattcttt
     2041 tattgaaaat gacatccgaa tcgatataga aatatcggtc gtcctcacgc gaatgatctt
     2101 cttctaaata tttccaccat aaatatggtt taatcgaagg aatatactct ttgtcgtccc
     2161 gcagatcatc gtacacatga acttcaacac catattcctt ctcaaaaaaa ataggaatct
     2221 gatcatcgtg tctgctgaaa agcaatacga tatctttgat tcctagtttc ttcagattag
     2281 ttaaacaaac ttcaagctcc catttaaacc gattgattgc cggctgacaa agaatatact
     2341 tcattctgat cacctacgct tgtgttgtag ttgttgtggt tgttggtttt gtagttgtag
     2401 tagtagttcc caaagcgcta atatctaata caatgaaact atcgttacgt ttaggttgac
     2461 cgtttgcata ttgtttagct agataaatgc gttcgtcttc aacaaaatgg tattcatctg
     2521 aagcttcaat ttttagtgta gatcctacac ccatgaagta atctgaggct accccaataa
     2581 ctgcttttcc ttctggcaca gccgttgact gcaaatctga aactggtact ggcaatactt
     2641 gtacgtattc tccattagca gttagtacag tcttagctgg gaatacttta gaccagtaat
     2701 cagttggatt cacaattagg accacatcag aaggattcac attacgataa atcggatcat
     2761 tcacaccttc gatattgaat tttgatagtc gcgccatcaa accgcccata gttacagcat
     2821 ctaaagctgt aataggttct gctttttttt cagcatattc tccgctagtt tgtttgctca
     2881 tgtcacgcat cattccgact ggcatatctt taccagtacc atcaacaatt gcttgttcta
     2941 atgcaattct caatgattct actaaaacag tacggacata acgatctaac catactggac
     3001 ctaaatcaag cattgcctta catacaggaa tataacctga tagcttgaac tgcttcatgt
     3061 taattacatc aaagccatta tctaaaactt ttttaacagc ttcgcaaagt ttaccccacc
     3121 atgctggatt gactccacgt gacacaatcc attctgttac accagttgtg ttaacaaaag
     3181 taattttttg caatagtgga tgagattgtt ctaaatcttc aaatacacgt tcaaatacag
     3241 tagctggcac taattcttcg acccctgcaa aaccttcgtt tttcactact tcgttataga
     3301 attttgtttc ttgtgtagtt aatacacgct gaccacggtt cattaatact aattgatctt
     3361 gattttttgc tgttgcttct tctaaaattt tatcctgaat ttccttagat aagcttacca
     3421 tagctgcgct aaaagattct tcgttaccat ctttaaaagc tttcatcaat tggtcgcttg
     3481 cagctgttac accttttaaa tttttaactg tcattatttt acatctcctt gtccaaatgt
     3541 tttatttaat gctgctgtaa atgcagcaat tttttctgct cttttttctt taacgtcatt
     3601 caaaatttct tcaacgcttt gttctttttt agcttcagta cctgagctat tttctgcatc
     3661 gataatttca tcgaccaatc cataactcaa agctgtttct gcatccataa acgattcttt
     3721 ttcaagaagt tcttgcaatg cttcatctgt gccattgaat cgtgttttat atgaagcctt
     3781 taccgattta tcaattgatt ccagttggtc agcaatcgta cggaagtcat cgacatttcc
     3841 ttctccgtat gtggaagcgc ggtgaatcat caattgtgca ttgttgtaga tttttatagt
     3901 atcgccagcc attgcgataa ttgaagcagc actagcggct aagccgttaa tcacaacgtt
     3961 aacttttgct ttatttgact taagtaagtt cccaatagca atcccttgaa atacgtctcc
     4021 accgtttgaa ttaattacta cttcaatttc ttcttgatca cctagactat ccaaaatatt
     4081 tttgattccc ttgtcagtat tcccttcaaa gaaccaacta gaaccaataa atccctgaat
     4141 aaaaatttgc ggtactgcgc cttcattctt tactgctaga aatgttttca ttgtcgtcat
     4201 tcgcctcacc tcctttcgat acttgttgat tgtttttagt tataaatatt tcatctgcca
     4261 tcgccttatc agagcgatca tttccaacgc gttctcttcc ttcgttgatt gtaaatactc
     4321 catttctaat gcctacatca atagcgtcaa ccaaatcttt gaagctagta atcttgatca
     4381 tagttgtatc cacacgtaca aaattccctg acaagtattc ttctacttca tagagactag
     4441 cgttaaacgc atcctgaata agttcagcaa tcggtatgat ttcgaacatt aaaaaagcgt
     4501 ccacttgatc cgataaccca ctcatgtctc cctttagtag gtttttcgga acgtgaaacg
     4561 ctgctgctgt catctcaaag atgtcgtcta ttaagttttt tatatctctt gaattgcttt
     4621 ggaagtttcc gctgaaatct tctaatgtgt actcattttg taattgaaat accgcacctg
     4681 cattatcagc ttccataaaa gccttaaatt gtgatgtcat cattttattg atttgatctt
     4741 gtgttgtatt gtcttgcggt cggaataaat tccctttcag tacgtatcta cgagcgttag
     4801 agcgcttgta aacattcatg gcactagaaa tgagtttccc atacgcttga taatacgcat
     4861 cgactagttg cctaatttgt tgatctgcgt attttatata gataacatca ctttctagaa
     4921 attctctatc aaggactatg ttgttaattt gcacttgaga aaacacatca tctttcaatg
     4981 catattctgt gacatcccaa ctatccgcaa taaatatttc gctagaatta ttagacggag
     5041 aaacgatcaa tacttcattg tagaatatta atctcctgat cagttttttt ctaaattctg
     5101 ttgcattatt tttcttatta ggagctacat tcagcctata gtaaagatca ttctttttat
     5161 tttttccatc ttcatatgac ttgaattccg ctttactcat cgcatttgca atcaaatcaa
     5221 tacaagtttc aatcgcaaat tttcgataca caaaatcaac ttgcaatt
//
LOCUS       DANKEF010000115         5193 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000115 DANKEF010000000
VERSION     DANKEF010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.117"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(61..279)
                     /locus_tag="PWZ48_002790"
                     /pseudo
     CDS             complement(61..279)
                     /locus_tag="PWZ48_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(545..739)
                     /locus_tag="PWZ48_002791"
     CDS             complement(545..739)
                     /locus_tag="PWZ48_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262397.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(839..925)
                     /locus_tag="PWZ48_002792"
                     /pseudo
     CDS             complement(839..925)
                     /locus_tag="PWZ48_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
     gene            2091..3131
                     /locus_tag="PWZ48_002793"
     CDS             2091..3131
                     /locus_tag="PWZ48_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HDL2262398.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
     gene            3955..4287
                     /locus_tag="PWZ48_002794"
     CDS             3955..4287
                     /locus_tag="PWZ48_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HDL2262399.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            4906..>5193
                     /locus_tag="PWZ48_002795"
     CDS             4906..>5193
                     /locus_tag="PWZ48_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HDL2262400.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLS"
BASE COUNT         1881 a          716 c          870 g         1726 t
ORIGIN      
        1 aggtattttt attatatata gatttaacat atgtcggtcg gttgattgat atagctaaag
       61 ttataactca accgaagccg attcatctac acgaataaag ttgcgagtgt tctcggctaa
      121 ttcataaaaa ttgttatgtg ttccatatag caatactgga ataactttat tttcttgaat
      181 ctcataagca actctataca cggtgaccgc atacttaaat ccattagacc aattacctga
      241 taaacctcca gttttttgct cttacttaaa tggatcaatg gcaatttcat cacaaaccta
      301 tagaggatga gtgacttctg cctaattacg ttaaaaaata ttgaaagtaa ctctatgtag
      361 caaaaaatat ctagaatttc atttcctcaa aactgatcga tattgtatct aaaaatatat
      421 actacctaaa ataggtagta tatattaaaa tatgtgcttt tgttagaaaa atcaatacat
      481 aaattatcat atctgtatat gtcatttagg tatttcagtt atgttataat agttttgctg
      541 tctgctaccc cggcctaatc tggcaagggg aggaggagat ataatgaaat acctttttga
      601 ttttgttttt gcaatcaagc cacttgcaga tgtagtgtgc agttacctct gcaaatggct
      661 tgattgcaaa attgccaaaa aagacagtaa gcctacccgt taattctata atatagaaac
      721 gtaaaaaaaa ccccatcagc ctgcacgctg atggggtttg gtgatataat gaaatacctc
      781 tttgttactc ataattgtac cataattagt cacgaatgca accaatagaa aaaactactt
      841 agaacggtag atcatcgcta ataacaatgc attgtccacc aaatggattt tgtgaagcat
      901 taaatacatt gttattaatt gtcgctgctt ttaactaggc ctctcccctt ctattataaa
      961 agaagatata tatcatttct taattaccta ctaccttaaa ccaaaattta agtactacct
     1021 aatttaagta gtacttaaac aatctaaaaa atagttatcc caattgttat gcttttataa
     1081 tacgattatt tgtaacaaaa tgaatattat ataattaaac actaccatat ttaggtagtg
     1141 tttaattatt agcaaaacaa aaaatattta ttctattctc ttaacttcaa ctaagttatg
     1201 ctgcacacct ttatataaat catatgcaac tcattagcta gacaaactac aagcatgtag
     1261 ttttatctgt taattatgta ctacctaaat aaagtagttt ataattagca agtatatata
     1321 tttaaaaatt taagattaat ttttttatga cttgtacaaa tttatctgat attctcttat
     1381 taagaactac ctaatttggg tagttcttat tttttacatt ttaagtagta tttaattgca
     1441 atttggtagt atttttttat tatcttattc ttagaacggt agtacttaat ataaatatag
     1501 gtagtatcta atttaagtat taatggtagt atctaaatta acaggtagta tgtaactact
     1561 ttaaagtaat ttttttcaga ttaacacagt agtatcttat taaataagtt aattttaatg
     1621 gtagtacata attattatat atatctgaca ttggtagtac ttattgtatt ttttagtagt
     1681 atataatcta ttgttttcta gcctaaagcc ttgtcttata agaataatat aatatatact
     1741 actgaaataa ggtagtatat attactagta atggtagtat gttttatatg cataaggtag
     1801 ttcttaattt attttttgtt ttgacactgg tagtacttaa ttatctttaa ttttaaaact
     1861 aaattttatt catacgtttt tctggtagta tttaattata aataatattt atttaaagta
     1921 gttcttaatg actttaaagg tagtacataa ttcattttat ttctgatttt aggtagtatt
     1981 taattaaaaa acagttttaa tgcgtttgca ttttttattt atctttgcta ttttcgaaaa
     2041 gagtaggtaa ttttacataa ataaaacaac gtgaaagtag aggatctaca atgactgatt
     2101 ttaaattttt taaggcggat agagtttaca acgaattatt ttatcaattt ccaaaagtct
     2161 ttattgtttc tgacgaatac aaaaaaatga aagattcaac taagattgcc tatatgcttt
     2221 taaaagcaag attagagatc gcaatcagca aacggcaaat cgatgaagaa ggtaatgttt
     2281 attttactta tacgacaaat gaactatgta gagtattaaa ctgccaaaaa caaaaagcga
     2341 tagcaatcaa aaaagagttg gaatcctttg gtttattatt acaaaagcag atgggattta
     2401 acaaacagtt agggaaaaat aatcctaata gactatatct agcagaatta aaagtctcag
     2461 aaaatgatat ctacttactc gaaaaatttg atagagagaa tagggaaaac gttgataaat
     2521 cagagggtat gaaaatcata cccaccctcg acgaaaaatc agacgctgaa tcccttgggg
     2581 ctcaagaggg tatgaaaatc ataccgtgcc aaaacgttga taaatcagag ggtatgaaaa
     2641 tcataccaga acttaataat aatatattag acactaatag acacaatata gacactgaaa
     2701 aagaccgcct acaagatcaa ttgttgttag acaattttga gacaattatg acaaacgaca
     2761 gcattgctac gtttgtccct gaacgagtat taaatttgat aaaaacattt tcttcaagtt
     2821 acagtgaagc tcaaaaaacc gtccagacta ttcataatgc aaagaaaaaa gctgaaatag
     2881 aaagtggtat ttcgatagtt tttgaagaac tcgatagtta ttatgtcaat gcagaacaag
     2941 aattatacac gacactgtta aaagcctatc aaaaattaaa aaccgaaaaa gtcgaaaata
     3001 tccagaacct gatttttgtc tatgtaaaaa attggtttat cgaaaaacca atagctgcta
     3061 aagtatcaag tgaaaaacgt ttgaattatg aaagctcccc aagcactatt acgaaagact
     3121 ggttagagtg aaactttact gacaaagtga ttgttgcttc atggagtaac attaaaatta
     3181 aaaatttttt attattctaa ttatattacg aatattacaa aataatagct attttaatag
     3241 aatttataag ttaaaatatt atgataaaaa tcacaaacgt agccttttac gtcataagtg
     3301 ttaaaagaaa taattaattt caaatataga aaagggaacg tatgtttgca tactcctttt
     3361 ttttgagtta taataatacg cgaagagaga taatttcatt atttaaacaa gctaaaaatc
     3421 ttgatattac ttcttttcag gcaatacgaa tattattcgc ttacattttt tgtataaatt
     3481 taattgtctg ctatgcttgt gatgaattga ttttcgttct gcacttttgg atgataaatt
     3541 tggacaattg aaaatacaaa aaaaagaaag gcggtatgaa ctcggaaggc tggtaacctt
     3601 cgtcagcgtt catacacggc attaagattt ggcccttaat gcgttaacct ttcagtgtta
     3661 cggtattacc cgcaatggat ttagtataac agatattcaa aagtgatacc actttttttg
     3721 gctgtgttaa ggttaaaaat acgtgatatt acgatgaatc tatgcctaag cacaatgtta
     3781 taaaaatgtc cgtgtggctc tttgccgtat gacgaacgct gaaattagag ggaacgcatg
     3841 tttggatgcg ttccctcttt tttgtttgtc atgaacgaaa atcaattctc tatttttgaa
     3901 aaataacaag aaaaacttgt taaaaatatt taaagaaatt gggtggttta ttttatgtca
     3961 tatacaggaa ttttgagtct caaagatatt tgtcactacg gtaaacggtg taccgctaca
     4021 gagaaaatca cgaagaaact atcaactggc caaagtaaaa ccgttgtgca gtgtaagaaa
     4081 tatataatcc agaaagacaa agtctcagag gaaatgattt attacattgg gaaacaaaag
     4141 caaataattt taaaagatcc tatcccctta aaagagctat atccaacaat caaacatgtt
     4201 tatgaccaaa acggtgtact gattggtcga agaaaaaatg gagtcttgcg ttgtaccgca
     4261 aaagggatgg ggcgtttgat cggctgaatt gatcattctc ataacttgta ttttatcgtt
     4321 tttcgcttgc ttgaaaatat tgtcgagact tctaaaggaa cttagagtca ctagaaaaat
     4381 aaggagtaga cttgatctat caacaattcc cttcggggag tgacggggta ggttttaacg
     4441 attggtaacg taaaaaattg gtcaatcgag taaatgcgct tgtttggata gtggtgaaat
     4501 gaatttgtgt aaataataag cgcagagtaa acaagaggga atttagaagt tagacaagtt
     4561 agaatagcgt ctagattaat catgagcagc tactaaggga tctagacttt gacgcaaact
     4621 catggaataa ggagtaggtc ttctcgtaat ggggaggacg cacaagaaat tgtgtcgtgt
     4681 gctgcagtac atgtagatac ttgcgaggac gttgattatt ggatcaatcg agcatttacc
     4741 gctgtctact agggtttctc gtttcggacg ccataaacga gtcgaaattt tttatgtatt
     4801 tttaagtaaa cgaaaaacga taaaatatga acaagtcctg aataaagtga aaaaattgat
     4861 ataaagcata gagaaaatga aatttacgta atggaggaat gacaaatgaa aaattatgat
     4921 ccgaatatac gattgggcac acatactatt aaggtttctt tccaacgttg ggattacaaa
     4981 ggatttgtga cgtttagaag gggcggaaat tgtaaaggat tagatgtttt agcattagac
     5041 gaggatgatt tatacgatca aacattgact gataatccaa ttggattcgg cttgctacct
     5101 gaagatgatg aaggtaatga atggtttaaa atgacgctta tgaatgataa tggagacgaa
     5161 ttatctgttg aagatacttg gtcatatttg agt
//
LOCUS       DANKEF010000116         5025 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000116 DANKEF010000000
VERSION     DANKEF010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.119"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            283..522
                     /locus_tag="PWZ48_002796"
     CDS             283..522
                     /locus_tag="PWZ48_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262401.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="PWZ48_002797"
     CDS             complement(884..1144)
                     /locus_tag="PWZ48_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262402.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="PWZ48_002798"
     CDS             complement(1329..1826)
                     /locus_tag="PWZ48_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HDL2262403.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="PWZ48_002799"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="PWZ48_002799"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HDL2262404.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="PWZ48_002800"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="PWZ48_002800"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HDL2262405.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5015)
                     /locus_tag="PWZ48_002801"
     CDS             complement(4548..5015)
                     /locus_tag="PWZ48_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262406.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1043 c          771 g         1682 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaa
//
LOCUS       DANKEF010000117         4932 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000117 DANKEF010000000
VERSION     DANKEF010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4932
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.120"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            85..972
                     /locus_tag="PWZ48_002802"
     CDS             85..972
                     /locus_tag="PWZ48_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HDL2262407.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="PWZ48_002803"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="PWZ48_002803"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDL2262408.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="PWZ48_002804"
     CDS             2274..2720
                     /locus_tag="PWZ48_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HDL2262409.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="PWZ48_002805"
     CDS             2993..4345
                     /locus_tag="PWZ48_002805"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HDL2262410.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="PWZ48_002806"
     CDS             4516..4803
                     /locus_tag="PWZ48_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262411.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1394 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccctttt tt
//
LOCUS       DANKEF010000118         4734 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000118 DANKEF010000000
VERSION     DANKEF010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.121"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(16..1101)
                     /gene="xerS"
                     /locus_tag="PWZ48_002807"
     CDS             complement(16..1101)
                     /gene="xerS"
                     /locus_tag="PWZ48_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2262412.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3177..3695)
                     /locus_tag="PWZ48_002808"
     CDS             complement(3177..3695)
                     /locus_tag="PWZ48_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HDL2262413.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1555 a          777 c          717 g         1685 t
ORIGIN      
        1 tattttttac tacatttaca aagcgttcaa agcatcaatt gtagctgcat tgtctatatg
       61 agtatataaa gttgttgtat ttgtacttgt atgtcccaat tgttgggcag ttaataccaa
      121 tgaatttgtc tgttgataga gtctagtagc caaagtatga cgtaatttat gtggactgac
      181 ttggactctg aatccttttg aatattttgc taccatccgt tcgattgcaa taccggatat
      241 acgcttggct tctccttgat aaattgctag aaataaagct ttttcgtttt ctgttacttt
      301 gtatcgttct ttccgaatag ctagataatt agctaaatat tccattgcaa atgctgctat
      361 atttactgcg tctctcttat ttccttttct agttactacc acattattct tttctaaaga
      421 taggtcctct aaattaatat ttacaacctc ggagagtctt aatccagatc ctaaaatcaa
      481 tgcattgatc gccaggtctc tttctacatc tcgttgataa tagatcaaag ctcgtttgct
      541 caaagttttt ttatattcat tggcaatata atctagataa ttttgtgtat cttcgtctag
      601 aaataatttt ttctcgattg cgtttgcacg ggcagctaac gtagttttat ctgtgatagt
      661 tgctacttga tccataggat tattttctaa gtatggtttt cctaaaggat tattggaact
      721 tttcgaaaga tatttaaata gaacctttaa tgctgtgata tttctattga tagtagaagt
      781 agatagacct aagttattat tatctttgag cttagttttt gttgtctctt tttttacatt
      841 ttcctttgtg cgacgtttca aatgagattt gtacaagttt agatcatagg cagttaactg
      901 tcttatcgag gtgattgaag tatcgggaac gatagactca ctcagaagcc attcataaaa
      961 gagcttataa ttttttagat attctaaagt agttcgaggt gattgatctt tatcctcacg
     1021 atcttgaaca aaatctttta tataatcggg catatgaagt atgattgtat ctattttttg
     1081 atatatttct gtatttttca tgttattcat cctttcgata agtaggggag aggagtatct
     1141 ctataattgg actataccaa ttatagagtg ttttgtcaat tggtatactc tgaaatttat
     1201 taatttattt caaaaaagca gataaatagg tatagattta ataatttaaa ataaatggta
     1261 tacatgaata ggaaaggaaa cagaaggagg caatcttaat tttttaaatt gtctaaatta
     1321 tatagaaaga acaatctttc tatataatta gacataagag agactacggt tgatttgata
     1381 ttaacaagcg tatatgaatg tatatacaaa taaaaaaggg gagggagaag ttaaagaaaa
     1441 ttcagagaag tggggcactc agtagtatta acaatttaaa aggaatttct taaagaaaat
     1501 gggttgcatc ctggagatac aattttttta gataaagaaa aaattcaagc aacaattcaa
     1561 aaaaatctta actgctgaga ttgatgtagc aatatctcaa tcatcaacaa agtacgatcc
     1621 tgcctttcaa aagcttattg ataaatgttt attcattata aaaataaaag aaaaatttat
     1681 gtcagctttt ttcatcaaag tggtaatgat aattcgtcta ttaattgtat tttttaggaa
     1741 atatggtata attgttttaa cgaaaaggta attcatgata caccaaaaac cactagctgg
     1801 cgggctagtg gttttctttt acgttctatt cagattaacg ggtaggctta ctgtcttttt
     1861 tggcaataat tttgcaatca agccatttac agatgtaatg cgcggatact tctgctaaga
     1921 ttgcaaaaaa catatcgaaa aggtaattca taatacaacc tcctcccctt gccataatag
     1981 gccggggtag cagacagcat aaatattata ccataactga aaatttcatt ttagtacatt
     2041 tcaatatatt gatgaaccag cacaataaaa catgccatga ctaacagaaa aaaacttatt
     2101 aaaccagata taacttgtaa acatccaact atatagaaaa cgtaaatttt ctatatagtt
     2161 ggattaagat aacgactatt tattttatca tatttttact ataccctata cttttacttg
     2221 gtatatttat gaataatcgc aaatttaccc ctatactact agtttgtaag ttagattaat
     2281 aaaaatacta aagttatttg ttcttttatt aaatacacct acaacttata aattgatggt
     2341 atttagataa cacgaaagaa taaaaataac agctgatagt aaagtttgtt gacgattgct
     2401 actcaatcga aagtgagtta gtgcgtatga acttgcttct gaaaacttat gaaatgaaag
     2461 accttttaca tcttgcagaa atcgttactg ggattaaact aagcaataaa aactaatgtt
     2521 cccctcattc tttgaacttc tactatttga tttatttatt ctgataaatg acgatttcac
     2581 cttcaggatc ttcaaatccc gtatagaatt tatttaagat ctcttcagta tcagtaatag
     2641 gataggcgat aggccacata ccctttgatg gttagaataa actgctcaaa atctgtatcg
     2701 tatgttggct ttagcatatt aacaaaggct atctccaaat tataaaccat aagcgttgaa
     2761 tcgttcttaa caatttcaat tcttcctaac ccaaagtcag aagatataat taccggactt
     2821 gccttaccct tcatgtgggt tgttgagatt ccatatttaa agggaattgt cggtactatc
     2881 ggcggtctga aaaacatccc aaaaataaaa aaatctttcc cctgctagtc tcctaagtcc
     2941 ctttgtagcg gatatggcac cataatctca ttttatcgaa gtgagtcatg gtttatctgt
     3001 tggccaacca aaattgatac aatctaacga tgcaccctct acggcattct aggggagtgc
     3061 acttgcattc acccaataaa aaccggcagt tagcaaagaa cttatgtagt cgtatgttct
     3121 tactgttaac atacaagagg tctcctttcc acaattgctt agcagtaaac gggaatttac
     3181 aaagctaatc tcttccattt tcttatcttt gttctaaaag tcccaaatgg agcaactgta
     3241 tttacatgaa taaatttata tacttcccat acagccgttt tggttgcctc gtctgcccat
     3301 tttcttttgt gtgcagtgaa tagatcttca tcactcattg aatcaatcat catgttaata
     3361 tcatcaatat tacgattgag aatttcctct aattctttca atggtagatg tgcataggta
     3421 tttgtaaacc acttatataa ctctcctaac tgattccatt taaattgatc agaaggcgtt
     3481 ttaacagata atccatttct ttcatcattt tcccatttta gaattaaatt cgtccaacca
     3541 acttgatatg ctaaattttc agcaggtgtt ctatccactt cctcaactct tttatccttc
     3601 aattcttcag gaatagtttc aaattctaaa atatatttag tataagtcct atttatttct
     3661 ttttttaatt cttctttatt tttatatgtt ctcattatat tacctccata aattcctaat
     3721 tgtctccttt aattcatcta taatttcatc cacgatattt taatccgttc tacaatgcat
     3781 atctgatatg cattgactgt ctaactcata tgtgaatgat ttataaaata tcccaacgtc
     3841 tcatctaaag aaatgttgat cgtcagcaag gagctaaaaa atacgaatat atttcttttg
     3901 tagtaaaatc ggactggttt gcatatcatt ttcttcgtaa atatcaaaat catgttaact
     3961 actatcaatt actaataaaa actaccactt taagcgttaa gaactgttaa gtttagttaa
     4021 gtgaggatag ttcacaaaat ccattgttaa acttcacgaa agtggtaaaa ctcaaaatgc
     4081 tttatatagg attacagcat cgctctttct tcgattgcgc gttgggtaaa aaatattatg
     4141 aagtaaaaat tgacgatgac acgttcctta cggctcatca gatgaaacag ctacaaaaac
     4201 gaaatgatca actcgaagaa aatttaattc taaaaaagcg attgccatat tcacgcctcg
     4261 ctcgacgaac tattaaaggt caagatttac ctgaagatca tcctttttag gaaattgaag
     4321 ctttttatgt tgcacaatgc atttataata ctacaatgat gttttcacca gatattcttc
     4381 tagggtgcgg tgtaatgaaa cagatacact tgcaaaagaa aattcaccgc gaatttacta
     4441 aaattatgaa taattatgtt aatcttccga aatttttttc caaaattaag ggatcaagct
     4501 ggtgttatag attgtttaag gttggcaaaa aaataacttc tttttaaaca attatagaaa
     4561 accgatactc tgttaagttg gttgacacgg tgtcgatttt tatgactttc ttattctgaa
     4621 acttcactaa cggacagtac ctaaaaagtc tatagtatgg acgtttacga gtactatttt
     4681 atcaaaaatg ctgctcattt caacttatat gttttagtga actaccccca ctta
//
LOCUS       DANKEF010000119         4698 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.122, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000119 DANKEF010000000
VERSION     DANKEF010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.122"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..142
                     /locus_tag="PWZ48_002809"
     CDS             <1..142
                     /locus_tag="PWZ48_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type II toxin-antitoxin system YoeB family
                     toxin"
                     /protein_id="HDL2262414.1"
                     /translation="SFEKLKPTHEGRYFRRLNRQHRVVYKVDEDVHVVEIYSARTHYE
                     SQ"
     gene            219..548
                     /locus_tag="PWZ48_002810"
     CDS             219..548
                     /locus_tag="PWZ48_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2262415.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            538..825
                     /locus_tag="PWZ48_002811"
     CDS             538..825
                     /locus_tag="PWZ48_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2262416.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKTLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            1111..1968
                     /locus_tag="PWZ48_002812"
     CDS             1111..1968
                     /locus_tag="PWZ48_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2262417.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1969..2517
                     /locus_tag="PWZ48_002813"
     CDS             1969..2517
                     /locus_tag="PWZ48_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262418.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2777..3505
                     /locus_tag="PWZ48_002814"
     CDS             2777..3505
                     /locus_tag="PWZ48_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HDL2262419.1"
                     /translation="MKKFKKKIYLGLVVGVSMFGFMAHTKQVTADTNQKERIMSQLDD
                     SQIEYVPNELIVSLKATQRISTEKNKPQNLLGVKVMVEKDLTLRTNESDYNKRDQVLL
                     VKLNEEISVEEAVRTFNASDMVNYAEPNYVVELEPLNDVEEPNTTDSLGNRSIILNDP
                     NWSKQANLREISMPSAWEETKGSKDVKVAVIDSGIDYQHEDLSGNVDESLRYDFFDDD
                     TDPMDTMGHGHILPGLLVLKQTTN"
     gene            3535..4644
                     /locus_tag="PWZ48_002815"
     CDS             3535..4644
                     /locus_tag="PWZ48_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HDL2262420.1"
                     /translation="MVPIRVFGSSGGTSLAILTEAINYASQKGFPIINFSIGSVVYSS
                     TEEAIKNYPGLFVAAAGNNGIDMTDKPFYPASLDIPNIITVGNSSGYERSSDSNYSKT
                     GVDLFARGFRVFSTFLGSKYTYMSGTSMATPHVTGSAALALSLDPDLSTDELKKIILN
                     SVDSFYELSDLCLTGGRLNTNNVVRQVTGKLTASNITLRLNSEWSDEIAKEMCHVHWI
                     DQSGNDLTDQVVVLKNEVDTSNFGDYEVVFASPDLTDQISVTVTVPRLRKLTLGKETA
                     TIDIGSAWDSVIAINTFQVKAIDDFGNDVTNQVQVTINADIDTINSIQAVIKFEVPEL
                     GLAQIGRLNITAKNSTINGLNEVSIEHVKKIMSFS"
BASE COUNT         1707 a          622 c          971 g         1398 t
ORIGIN      
        1 atcatttgag aagttaaaac ctacacatga aggtaggtat tttagaagat tgaatagaca
       61 acaccgggtt gtttataagg tagatgaaga tgtacacgtg gttgaaattt attcagcaag
      121 gacgcattac gagtcacagt aattacttgt aaatacatta tctcgtggta taatgtattt
      181 actaagtaat tacatactgt tagaaatgag ggatttaaat gattacaaaa acaagaaaac
      241 aaggcaattc aatcatgttg acagtaccta aagattttaa tgttcctaat ggagtcgaag
      301 ttgaagcaaa gttggtagag aatggtattc tttatgaatt tgttgaacca caaaaagaat
      361 tttatgattt tagcgaagat attttgtctg atataattgc agaaggctac gataaagatg
      421 agattcttgt agaatttaag aatagaaaaa acaaaatgca ttcttccttt agagatattg
      481 ctgaagatac acttactaat tctaaagtaa tgactaaaga ggagttggca acagaaattg
      541 gcttatgaaa tcgttccttc aaaacatgtc attaagtatt taaaaaagct aaaagagaag
      601 acattgaaag aaaaattttt gaatttaatt tatgatgaaa ttgcgattga tccatataag
      661 ggtgaacaaa aaactgggga tttatcaggt atttggtcta gtgggttcaa atatgcgggc
      721 acaacatata gagtagctta cgagattata gaaaacaagg ttattccagt attgttatgt
      781 ggaacgcatg aaaactttta tgaacagttg aaaaaattaa tctagtgatt tgttgtcgtt
      841 cttaagaagg acggcttctt ttttatcgaa gatctgattc atctacatga ctaaagtcgc
      901 gagtattctc tgcttctcga taaattaaat aacgtcatta ctttgaggaa agctataaat
      961 caatatattt cagcttttcc ttatttatgt actacctttt acttattaat tataaggtag
     1021 tacatatata tctttttttt ggtagtaact aaaaataaat atgttataat agtatcaaat
     1081 gaatattgct aaaagaaaag aggtaggaac gtggtagcga agaaatatgt aatcggtaat
     1141 tttaaaggtg gcgtaggtaa aagtacgtgc gcgcaaatgt tagggtttga atcagcagtt
     1201 aatgaaggaa aaagaacctt gataattgac ttggatatgc aaggaaatac ttctgatgtg
     1261 atgaacttaa cgcatatgaa cttcactaac gaagaaggag gcggagaagg tgaaccgatt
     1321 gtttacgaaa atactgtgac tgacgtgttg ataaacaatc ttccacctaa agaggctgtc
     1381 tataaagtaa tcaataacct ctatatactt ccagcagaca tgtcctttga aatgtatgat
     1441 gattggatca aagaccaata taatgattca ttatcccaat tcaaatatat gaaagaaaag
     1501 ctagaaccat tatttgatga gtttgacgtc atctatttag atgtcccacc ttctatttct
     1561 gtatatagta aatccgcgat gtacattgcg gactgggcaa tcgtcgtctt acagacccaa
     1621 gtaaaatcga tgagaaatgc gatgcaatat cttgagtata tggacttttt tgtgaaagaa
     1681 tttgatacaa atttatatgt tgctggtgtt attcctttca tgttggaaag cggtgacgca
     1741 gtagatcagg aaatgtataa acaagcccaa gagatttatg gggaacattt gattaaaaat
     1801 gttgtactca aaaatgcgcg cttaaaacga tacgacggat caggtattac gatggagaag
     1861 acaaagaaag gtctcttaaa acaatgggat cgcaaaagcc acgaattatt tatcagtatt
     1921 ttgaaagaat tggaagaaca cgagaattgg tatgcggagg tggagtaatt ggctgacatc
     1981 aaaggacttg atatttttgg gaacaacaaa ggactcgcca aaataaaaaa agaagcagaa
     2041 gcaaagaaaa agcagcaagc aattacacca tctgaaaata agcaattaga agtgcaccaa
     2101 gttcaagaaa taaaaacttc ctccaatgtt gaaagacaga gtagaggaag aacaaaaaaa
     2161 gttggcgcac caatcagaaa gtttgataag gtttattcca ccaagcatcc tttaaaactg
     2221 tcgactttgt taaatgcgac ttccagagtt ttagtagaaa agtatgagac taatatgaca
     2281 agagatgaat tgcttagaaa agctttagat gagtatatta aacagaatct tacgcaagaa
     2341 gataagcaag atttgtttaa tgacgtggtg aaagaactag acttgttcag agaaaaatat
     2401 ccaattgttc cagaattaga tgatcgtgga gaaataataa gaactaccag tcagataaaa
     2461 caagataccg aagactcact gcgcaaaggg tggggaatga ataataaagt tagataataa
     2521 tcaaagaaga actaagtaac tacctttaaa cggtagttac ttagttcttc tttgatttgc
     2581 gtgaaataga ggggatttat ttttctggtt acgtgattaa aaaatccaaa aaatgagcat
     2641 aggaacggtt atgattttag ggtgtgtttt aatgagatat tgtgatttca ttaacaagtg
     2701 tatgataatc tgttgttgag ttagtaaata agtaatagag ctaactctaa aataaaacta
     2761 ttaggaggag atgtaggtga aaaagtttaa aaagaaaata tatttaggat tggttgttgg
     2821 ggtgagtatg tttgggttca tggctcatac taaacaagta actgcagata ctaaccaaaa
     2881 agaaaggatt atgagccaat tagatgactc tcaaatagag tatgtaccca atgagctaat
     2941 tgtcagttta aaggctacac aaagaatttc aacagagaaa aataaaccgc aaaatttatt
     3001 gggtgtaaaa gttatggtgg aaaaagattt gactcttagg acaaatgaaa gcgattataa
     3061 taagcgagat caagtattat tagtgaaatt gaacgaagaa attagtgtgg aagaggcagt
     3121 aagaacattt aatgcttctg atatggtcaa ttatgcagag ccaaactatg tggttgaact
     3181 agagccgttg aatgatgttg aggagccaaa tacaactgat tctttaggaa atcgatcaat
     3241 tattcttaac gatcctaatt ggagtaaaca agctaattta agagagatta gcatgccatc
     3301 cgcttgggaa gaaaccaagg gttctaagga tgtgaaggta gcggtcattg attcaggaat
     3361 tgattatcaa catgaagatc tttctggaaa tgtagatgaa agtttgagat acgatttttt
     3421 tgacgatgat acagatccta tggatactat gggacatgga cacatattgc cgggattatt
     3481 ggtgctcaaa caaacaacga attaggtgtt gctggaataa attgggatgt tacgatggta
     3541 cctatacgtg tatttggtag tagtggaggt accagtttgg caatcttaac tgaagctatt
     3601 aattatgcat cccaaaaagg gtttccaatt atcaatttta gtataggtag tgttgtttat
     3661 agcagtacag aagaagcaat taaaaactat cctggattat tcgtagcagc agctggaaat
     3721 aatgggatag atatgactga taaacctttt tacccagctt ctttggatat acctaatatt
     3781 attactgtag gaaattctag tggttatgaa cgaagttctg attcaaatta tagtaaaaca
     3841 ggtgttgatt tattcgctag agggtttcgt gtttttagta catttctagg aagtaaatac
     3901 acgtatatgt caggtacctc gatggcaaca cctcatgtca ctggtagtgc tgcattagca
     3961 ttgtcactag atcctgatct atctactgat gaattaaaaa aaatcatttt aaatagtgtt
     4021 gattcatttt atgaactatc tgatttatgt cttacagggg gaagactaaa tacgaataat
     4081 gtcgtacgac aagtaacggg taaacttact gcatctaata tcacattaag attaaatagt
     4141 gaatggtcag atgaaattgc taaagagatg tgtcacgttc actggattga ccagtcagga
     4201 aatgatttga cggatcaagt ggttgttcta aaaaacgaag tggatacatc taactttggc
     4261 gattacgaag tagtatttgc atcacctgat ttaacagatc aaatctcagt tacagtgacc
     4321 gtacctcgat tacgtaaact tacgctagga aaagaaacag caaccattga tattggttcc
     4381 gcatgggata gtgtcatagc cattaatact tttcaagtga aagctataga tgattttggg
     4441 aatgatgtta caaatcaagt acaggttact attaatgctg atatagatac aattaattca
     4501 attcaagcag tgattaagtt tgaagttcca gaattaggct tagcccaaat tggtagactg
     4561 aatattactg caaaaaatag tacaatcaat ggattgaatg aagttagcat cgagcacgta
     4621 aaaaagatta tgtcgttttc gtagaagaat ttggacattt agagggtgat acgattgtcg
     4681 gcatccacca taaaagtg
//
LOCUS       DANKEF010000120         4633 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000120 DANKEF010000000
VERSION     DANKEF010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4633
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.123"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            174..518
                     /locus_tag="PWZ48_002816"
     CDS             174..518
                     /locus_tag="PWZ48_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDL2262421.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="PWZ48_002817"
     CDS             519..938
                     /locus_tag="PWZ48_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HDL2262422.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="PWZ48_002818"
     CDS             complement(1030..1380)
                     /locus_tag="PWZ48_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262423.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="PWZ48_002819"
     CDS             complement(1346..2677)
                     /locus_tag="PWZ48_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HDL2262424.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="PWZ48_002820"
     CDS             2933..3499
                     /locus_tag="PWZ48_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDL2262425.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="PWZ48_002821"
     tRNA            3644..3731
                     /locus_tag="PWZ48_002821"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            4065..>4633
                     /locus_tag="PWZ48_002822"
     CDS             4065..>4633
                     /locus_tag="PWZ48_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL2262426.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1683 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata
     3781 agaacggcca aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca
     3841 gatgcaagct tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac
     3901 agccccttat tgttgtactt ttaacctaca atattttttg attccccctg agtactattt
     3961 tttataaatg ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat
     4021 gtaagataaa agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca
     4081 cttatgcaac gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg
     4141 aagtggtcac tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag
     4201 ataaacatta tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg
     4261 aggctacaaa tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt
     4321 ataaagaatt acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac
     4381 tagggcaatg ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca
     4441 atatcgattt tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag
     4501 aaaaatcgat agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg
     4561 gtgtgttgac aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct
     4621 cgctatcaaa tgg
//
LOCUS       DANKEF010000121         4593 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000121 DANKEF010000000
VERSION     DANKEF010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4593
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.124"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            189..2858
                     /locus_tag="PWZ48_002823"
     CDS             189..2858
                     /locus_tag="PWZ48_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HDL2262427.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2949..3569)
                     /locus_tag="PWZ48_002824"
     CDS             complement(2949..3569)
                     /locus_tag="PWZ48_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDL2262428.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3683..4222
                     /locus_tag="PWZ48_002825"
     CDS             3683..4222
                     /locus_tag="PWZ48_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262429.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4219..4557
                     /locus_tag="PWZ48_002826"
     CDS             4219..4557
                     /locus_tag="PWZ48_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HDL2262430.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1423 a          790 c          860 g         1520 t
ORIGIN      
        1 aaaattttta agggtgggac ttttgattcc tacccttttt ttgcgtttat ttttgttata
       61 cgaacaaatt atgaagttat ttattctaaa ttttattttt ttgtgaaaat tagactcttt
      121 ttaagcagag aaaaacaagg atatgataaa ctgaatacaa cttcatagaa atttggtgga
      181 aaataaatat gaaaaaaaat ttaaagtcat tctttagaga aaacggacgc tacgcagcag
      241 ctagtttcct gattccgttt ttgattttag cacttgttta tctaagtatc gggatctatc
      301 ctggaagcag cagaagtatt ttggcaagtg atgccttttc acagttttct aattttcatg
      361 ccagcttccg aaatgttctt cttggaaagc aaagtatttt ttatacatgg aatgcttctc
      421 taggattgaa ttatttatct ttaatttctt actatctagg cgggatcttc acgccactgg
      481 tgttcttttt tccaaaccag atgatgccag atgcactgta tgttctgact ttgcttaagg
      541 tcggctgtgc cggattggct ttttggtttc ttgcacgtac gttcaagatt cctcgctggg
      601 ggcatgttgc acttagcgtc agttacgcat tgatctcatt tatcactgca cattcggaga
      661 tcatcatgtg gctggatgca tttgtttatc ttccattagt gattttgggg attcatcgtg
      721 ttatggatct taaacggccg aaacttttat ttatcagtta tttactcttg tttttatcca
      781 gtttttatat gggttttatg attggcgtat tttctttctt atattttatc gcccgtctat
      841 tgacaaactg gtcagaatat aaaaaaacga ttgttcctta tggtatcact tctttactgg
      901 caggcggcgc ttcaatgatc attgtattgc cagcagtgct ggatcttcgg tcaaatggcg
      961 aggcactgag tcagatcact acgttgaaaa cagaagcgac ttcttatctg gatctaatca
     1021 tgaaaaatat gattggtgtt tatgacacga caaaatacgg atcgattccg ttcgtttacg
     1081 caggtctgct accattagtc ctctgtctgt tttattttgc cagtcggaaa gtaccgttga
     1141 aaaataaatt tttgtacggc agtcttttcg ccatacttat cgcaagtttc tatcttgttc
     1201 cattgaattt gttctggcat ggtatgcatg caccgaatat gtttttattc cgttatgcct
     1261 atctgttctc tttcttagtg ctcctgcttg caggaaaagg atgggaaaag atagaacaag
     1321 acgatcgtgg gctgttgtta ggaatcattt tggtcgtagc tgctctcttt gcggtggcat
     1381 ggggcatcaa acctgaagga agctacacct atgtgacgat gacttccttt gtattgacag
     1441 tggttttctt agggatttat gcttttgctg tgggatttta tcagcataaa aaactttcat
     1501 tcaatcatct gagtatattg ttattattga tcatgtctgc tgaagcactg gtcaatacga
     1561 acagtatgat ccatggcatt cttgatgatt ggaattatgc ttcccgcagt ttatatactg
     1621 atccttatcc tgacttgaaa gctcttgtaa atgaaacaaa aaaagattcc gactcgtttt
     1681 accgcttgga aaatctggat ccagtatctg caaacgattc gatcaactat ggttacagtg
     1741 gtatcagttt gttttcttct ataagaaatc gtaattcttc ctcttacctg gataaattag
     1801 gtttccgttc aagagggaca aatttgaata tccgttataa caataatact ttgctaatgg
     1861 atgggtttac aggaatcaaa tataacattt ctaaaaacga aagcacgttc agtaaatatg
     1921 gcttccgcca agctggtcaa agtggcgact atacactgta tcagaatgtc aatgcattgc
     1981 cactagcatt tactgcaccg ttgtccgtta atgatgttga gcagccagtg acagataatt
     2041 tgaccagtca gacaaacttg atcaatacat tgtctggttt aaatgaacag tattttacat
     2101 tctatacacc tactttgaag aatcagttga atacgacgat cacaaagaca acgactggcg
     2161 tcacttactc agaaacagca gataaccagc caaaagagct cacttgggaa gtccatgtgc
     2221 ctgcaaatac acaagcttac ttaagtttgt tcccaaccaa ttttgcacaa ttagaaagtt
     2281 ctagtgcgac gatcactgtc aatggtacat cgcgaaaaac gcaaatcaac atttctggtc
     2341 aatactatga tcttggctat tatcctcaag agacaacagt gacatttact gcaagttttt
     2401 atggaacgaa agaagtaagt ttcatggagc ctaaagtcgt tggtctagat gttgtagcct
     2461 atcaagccgc aatgaatcag atcaaagaaa aaggtgtcga aatggaagcc actggcagaa
     2521 aagcttctgg gactgtaaat accgctgaag attccatgtt ggtgacaact attccttatg
     2581 acaaaggatg gaaagctaaa attgatggga aaccagtcaa ggtcagcaat ttcaaagacg
     2641 cattcttgat gataaaagtt ccagcaggta aacatactgt cactttcagt tacttgcctg
     2701 aaggatttac gataggtgca atcctttttg tcctatgtat tgcattgttt attctttatg
     2761 tctggtacct tgaccgcaat cgcccctttt atggcacgat tgatccagca atagaagaaa
     2821 aaccgcgaaa tacacacaga agaagacgaa gaaaataaag aaacgatgga atctgtgtca
     2881 ctcttctatt taaatggcaa aaatttcaat ctttaaatga caaaaaccac ctgatttttg
     2941 gtggtttttt atatacctat gactaaacta tctcttgctg cttcgacaac ttctacagtg
     3001 atcttatcca tttgattgat tcttaaaatc cgatcaatct gcgcaaataa acgatgaata
     3061 agcctaaaat ttccttttgt gatcttaatg atagttgtga tagcctcata atcagaaaaa
     3121 tcttctagct tgatatcaat tcctaaatct gcccatttgt attccaaaat atgatgtgtt
     3181 tcgtccttac taaggttatc aaattcatgt gcaaatccta tgcgagagta aagctgcgga
     3241 taacgagaca atcttttttc aataccaggc ataccgataa gaaccattgc cagatcattt
     3301 ctatcgtaaa tatctcttag ctgttctaaa tgctgaagct ttaaacgatc aatttcatcg
     3361 acaataatta agtcaattgc ttcataggtt ttaggcacag gaggatcttc ttgattgtat
     3421 tttagtacca tatgcattgc tttagtcaga cctattctac tagttattgt gttaatatcc
     3481 gaactgacac gagacatctt ttcagctggt acagtataaa aaactttatt tacacttaat
     3541 atttcatctg ttgcatttat caagaacaag aaaaagtctg aatattcaaa aaaaataacg
     3601 gtgtacattt ttaaaaggag atgtataatc gagatttaga aagcgttttc ctcaaaattt
     3661 aatgaattag gaagtgatta ctatgaaaaa attatttagc gcacttttta ttctgttatt
     3721 tggctttatt ggtattggga cttctggaaa tatagttaaa gctagcacga tggttgaaca
     3781 aacaagtgaa atggcaaaaa cttttagtca attaacacca gaagaacaaa gttacttcaa
     3841 atccaaagga tttagtgata aagatacttt ttacactcaa aagatagagc ctcaagatcc
     3901 gaaaacacgt ttttctgcaa atgtattgcg gttaaccgca tctactaaaa aaattactaa
     3961 tacgcagggg cggacagaat atattataac aagtcccaat tcggcgatgt atcgtatttc
     4021 tacacgcttg agtttaggta gtgcaaaatc atatactcaa acagtaaatc tagctaattc
     4081 ttatggttat ggtggaggaa tgtattttac atttactggg aaaagacaat attacagtgt
     4141 aaaattagct atgcaagttt ataatacatt tggtgtaggg tcagtctctt gcactgcggg
     4201 aggattgaca ataggaaaat gataatttct tactatgtct taaatagaag tgatgatgaa
     4261 ggactcttcg tttttcaatc attacatgag gatacgatgc taaaagagtt ttataatcgg
     4321 attaagaata aacagataga aacaaattta tattttttaa gtgactctat cttgcctttt
     4381 attagcaacc ctaaaattct taacttgttg cagacaaaag atttgaaaag tttaccgatt
     4441 attgtcattg atgaggaaat tgtaaaatat ggcgcttatc taagcgtaga agaattagaa
     4501 aaattaacta acattggaat gagtattcag gaggataatg gggtacatgg aacatagaat
     4561 gaatatgcac agttgagaac ggaatttgac att
//
LOCUS       DANKEF010000122         4477 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000122 DANKEF010000000
VERSION     DANKEF010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4477
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.125"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(832..1125)
                     /locus_tag="PWZ48_002827"
     CDS             complement(832..1125)
                     /locus_tag="PWZ48_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HDL2262431.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1528..2706)
                     /locus_tag="PWZ48_002828"
     CDS             complement(1528..2706)
                     /locus_tag="PWZ48_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2262432.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2810..3124)
                     /locus_tag="PWZ48_002829"
     CDS             complement(2810..3124)
                     /locus_tag="PWZ48_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262433.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3231..3446
                     /locus_tag="PWZ48_002830"
     CDS             3231..3446
                     /locus_tag="PWZ48_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262434.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3746..3988)
                     /locus_tag="PWZ48_002831"
     CDS             complement(3746..3988)
                     /locus_tag="PWZ48_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262435.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3975..4403)
                     /locus_tag="PWZ48_002832"
     CDS             complement(3975..4403)
                     /locus_tag="PWZ48_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2262436.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1418 a          841 c          663 g         1555 t
ORIGIN      
        1 ataaaaaatc atcaaataat cctttacacg ttattctaaa tttaattttt attattttat
       61 tgatgtatag aaagtttggg gctttttttc tctttttatt ctaaggttat tttagtcttg
      121 tcctttaata caaagatttt atttttaaat aaaaaatact ctttttttac gattattgaa
      181 aaaaaaagat aaaacttgat taattgaagt attgcttaaa tttggaagct atgagggaag
      241 aataaaagag ttaggacggg attatctact atttttcttt gttttaaata tttgttatct
      301 ttgaacataa aagagaaatg tgtcgactgc agtgttgtgt ccctcatcac gaataaacgg
      361 tgttcaatta gctgatcttg tattcactta aaaaatggta aagtacgagt tgctattccc
      421 cattttcaat tttctaacgc tcaggacaaa atactgtttt cacaacctca ttggaataaa
      481 agcatatata ttgtttcaag aacgaaatct tgttatcttt ttaaagggca tctgtccaaa
      541 atctaatttt aggaggtacg ttttggtatt tattcgtaca aataaaagag aattatcttt
      601 attcatgtaa cgcatccaat tgattttttc atgtacgctc ctggaacgat tttactgacg
      661 attgcttatt taactgcgaa tcgctcatta gcttttatca tagctatcca tattttgaat
      721 aacgttcttg gctttgtatt atagatacag atgacaaaaa aagttcaagt atgcggtgta
      781 gaaccaatat cgcatacttg aacttttttc aatgagtata aattaaaact tctaattttc
      841 aattcccatc atctgatatg cccgaccgtc tgaacttctt ttgaaaaagc caaatgtgat
      901 tagataacgt ctttgttcga cataatcgcc actagaaaat acgttcagca gtatcgaatt
      961 aacctctgtt tctgtataag tcactccatg gtcaaagcgt ttcaagactt ctttcaatag
     1021 gatgtatttc ttttttgttg aacttggtac ttgttttact tttccatcat tcatgaatgt
     1081 gtctaaaact ttcatctttt cttgctccgt ataagtataa accatcttct ttctcctttt
     1141 gattctaaga atactccatt tatgttagaa tattctggat tttttattaa agcggttact
     1201 cttaagaaag atattatcat agtgctcttt tttttactac tacttatagg tagatttatt
     1261 ctaatccatt attctcccct accaataatt aaagaaaaaa ctggatcagg gtgttcaaat
     1321 tacatgctaa gaaaaaagaa cccttgaata ccaagggttc tttctcagtc tgataacaat
     1381 gattaacgac ggatttcttt gatacttgtg aaaagtcgtt aaagtattga tataaaagga
     1441 tttttattac ctctgactac atattgacta atttgttaaa aaatcgtttt tcaagaatcc
     1501 tgtaatacca gtgctttcac cgttttttta actctctaaa gaactaaaag caatcagagg
     1561 ttttaccttt tcatttgacg ttaagtggcg ataaattttc gaagttgttt ggtcagtatg
     1621 gcctaagcga tctttcaaga cttcccagct acagtgttcc gctactaagc ttgccattgt
     1681 atgacgagct aaatgagtat gaaatggctt attaattcct gagcgattca cgcaccgttt
     1741 gacgtgatca tttacatact ctcgacgata aggaacacca cgtttactag aatcaagata
     1801 cacaaacaaa tatgtttctt ctataataaa cctgtaatgt tgcctcatct cacccaaagc
     1861 cttaatcaac tgttgtagct tttccataac tagaggagtt actagaactc gtctagcaga
     1921 actaactgtc ttagtagttt caattcgaaa atccttactc ttacctttaa ctgccgtttg
     1981 cttatagaac agaatttcat ttcttttgaa atctatatct ccaggctgta gagcaagcgc
     2041 ctcgctgact cggcagccag tgtaaaatag aacatggaaa agtacagaca tcggtaattc
     2101 ctcatgaaca atgccataat tcaaaaaagt tctcaattca tcgacagtaa gaaacttgtc
     2161 ctcaatatta tttatggctt cttttaaatc agttaccgtt aactgatatt ttggcattct
     2221 cacagacttc attggatttt cacttatcaa attctttctt tgacaatatt taaaaatcat
     2281 atttagaact tgaataagtg attgtaaact atttttggca tagccaacat ttgttccatt
     2341 aacgccacat tcctttaagg taaaaagcaa ttcttgaagg cactctggtg tcaaatcaga
     2401 caacttttta tcaccaatcc atcgaacaat aatttttaat tgttcctctc gcttatcata
     2461 ggttatttcc ttttgttcat ttaacgaacg gtaatattgg aaccattgct cacctacaac
     2521 actaaacttg tcattgcttg caacaatttt tttcttaagc ctttgttgtt tctccatttc
     2581 cagataagcg agttgcgcct cacgttttga attaaagcca cgtcttgttg ttcgatatgc
     2641 ttgaaagtac catgtgatcc taccagtaga actgatttgc ttcttgaatc gatctgtttt
     2701 ttccatttat tgattcctcc tattttgaag gaacccaaca agcagaaacc ctttggaaat
     2761 ctttctatct atgtcatcat tgtaccgcat cttagaaaga aaatgtaact tataaagatc
     2821 gaagtagtcg caatttttct cgatagtcat tcgaatcact ctttctcatc cattctaaaa
     2881 catcatcacg aaaatagtgt cctcgaatcc cgtaccaaca gggaggaaac tcaccaagtt
     2941 ttactaaacg gttcagtgta ctatcactta tctgaaatag tcgctttaaa tcagtatttg
     3001 ttaataccag aggtaaatga ctgactgcaa gcaatttttc cgtgtaatct tccagttttt
     3061 tttgtaaaat tgaatagaac tctatttcca gagaattaac atttgtttta acttccaact
     3121 ccatcaccct tctctctcaa aatcaaaacg accaccacgg taccccttaa aattgtaaaa
     3181 aaacactcca gtactccatc aaaacggact agaaaagagg aatttttaat atgaaacaag
     3241 gaattgcaga cataaaaata attaaagaaa ttcttgaaaa atctacggct aatgcaatag
     3301 cattcggaac aggaatcaac ttgagtacag taaaaaaact taaaagtgga gagagagctg
     3361 aagaaaaatt aaatcttgcc gatgctatta aaatcacaga atttggcatg aaaaatatgc
     3421 caaccaaaat cgaaatatgg aaatagtagt ttttctgatc tatcagctgt tttgccaaag
     3481 cacataggaa tctcacctcc ccccagtaat ggcgggccga tttggttacg gaagtatcat
     3541 tatcgtagtc gcgattctca tattaaaatt tgtcgcttaa aagctaacct tgtcatggcg
     3601 taactagaga atcaaaatct acatgtactg atagatttgc tattcatttg tcaaagagcg
     3661 ctaggctatt ttcagcctat ggaaatcctt tattttgacc cgataaaaaa cttccataga
     3721 atgaaaagga aagggaaaaa atactttaac gagttgtatc aaattcaagt atcttctcaa
     3781 tcaattttgt ttccagttgt ctacgcatat attcatctac atacataacc attccttttt
     3841 cttcttcata aactggtttc atggctaacc gtgaaatata attttgatat tttgacagaa
     3901 cgatggacat ggcaattgaa tcaccagaag ctgcggcttt gattgtcgtt agtggtatga
     3961 atggtatata tcgtttattt tgcttcatat ttgacaccca aatactttcc tagctcttcc
     4021 aaagccattt gtcgttggta ccaaacactg ccgagacttt tcccaataat atcgccaatt
     4081 tcacgatcat taagaccgat gaagaaggac ataaaaatga tttcttgtct ttgactaggt
     4141 aatgctgcaa tcgcatttgc caactcctca ttggaaataa agattttcat accaaggaca
     4201 ataaaagtac cactactatt caaatttggt tccaaataat agaactctgt acctaaccct
     4261 ttttgttcca atatttccat tgatatttgc cgttggcgaa atctagcgta cttcttttga
     4321 atatttaacg cctcattgca gactactttc ttacaaaaac tgtcaaacgt atgttgaatg
     4381 tgggtttcct ctgagtcgcc cataatattc tccccctcaa aaaattcatt caactcgttt
     4441 tacttctatc cctttccatt attactacaa tctagga
//
LOCUS       DANKEF010000123         4458 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000123 DANKEF010000000
VERSION     DANKEF010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4458)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4458)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4458
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.126"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            1056..1979
                     /locus_tag="PWZ48_002833"
     CDS             1056..1979
                     /locus_tag="PWZ48_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262437.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1995..2534
                     /locus_tag="PWZ48_002834"
     CDS             1995..2534
                     /locus_tag="PWZ48_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2262438.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3215..3388
                     /locus_tag="PWZ48_002835"
     CDS             3215..3388
                     /locus_tag="PWZ48_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262439.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
     gene            3793..4227
                     /locus_tag="PWZ48_002836"
     CDS             3793..4227
                     /locus_tag="PWZ48_002836"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262440.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT         1664 a          667 c          756 g         1371 t
ORIGIN      
        1 cattttctag ccactttttg tattgtggag cgtgggtttt gagggggatt caagggggag
       61 cttgcgcccc tttgacaagc ggttatttgc accaaatacg gaggtatttg gctagcaaat
      121 atagagcttg ccaaaccctt ttagtgagtt ttgtagaaag tacctttaaa ggtactttca
      181 cattacgaac gatacgtccg taggacgtac tgaaagggtg ggacttatga gcaaattaat
      241 gcaaaatttg gctagcaaaa aagctaaaaa atatatgcta ataatcgagc gaaataaaca
      301 ctttttcaag tgagcgaatc cgccatgaca aagctgaagc agtttatttc agctttgtca
      361 tggcgtgtgc cgactttttt aaaagtctag gcacacgagc aaatatcttt atcaagctct
      421 aatttgcgtt ttaagacgtt tgaaacgcct aagtatataa atatataaga ataggtttaa
      481 gagtgcttaa atcgaatttt agagggcatt tagacaattt tagggttgtt agggcttgcc
      541 ctgaccgcct gtaaggcgtt ttaagaacaa gataagggat ttatactagg actgtcttaa
      601 aatgtcttaa acgaacgctc agagcttctc aaagggttcc ttatgaacgg cttttagcaa
      661 aggagccgga ggcgactgcg cagtataatg tgagagcaga gcgatcacac ctttttagcg
      721 aaccgtaggg agcaaggttt ggaattggca gttttgataa attcgcgaac ctgtttagag
      781 aaaaaaggag tgatttttta ggcgggaaaa ttagcctccc ctctttttat atctcttttt
      841 atatctcttt ttatatctct tttatgtcgg ctgtgtcctt actctcccaa gcaattcaga
      901 gttttttcaa gtaggttttc tacgcttttc aagtaggttt tctacgcttt tcaagtaggt
      961 tttctacgct tttcaagtag gttttccgac attattgagt atttttataa agtatgttac
     1021 ttttatttca tactttataa aagtaggaga agcttatggc aaatgaaatt gtaaaatatg
     1081 aaaatagatt aaatcatatt cctctgagaa aatttaatag tcgtgaaatg aacttatttt
     1141 tttcaattgc ttcacgtgta cgtgaaaaag gtgtagatga gattattttt tcatttcacg
     1201 atttgaaaaa actaagcggt tatacagaac gtggggaaca atttataaaa gatcttagta
     1261 gcacgtatga caaattatta agtattaatg cttggaatga tgatggaaat actttaacta
     1321 aatttgttgc tttcacaaaa tatagcatta tacgagataa acaacttgtt ataatttcag
     1381 ttaatccaga atttaaagga ctttttaatc aattaaagaa ctggacaaga tttaatctag
     1441 agcaatttac taatttggat agcacatatt caaaaactat gtttcgatta ttaaaacaat
     1501 atcgaactca aggctggact gaattttcca aagaaaaatt ttttgattta ctagatattc
     1561 ctaaaagtta ttggaatagt ccgtcaaata ttgacaaatt tatcttaagg ccaattaaag
     1621 aagaactcac tcccttattt aaaggattaa ctattcgtaa aaaatatggt aaaggtcgag
     1681 gtaaacctgt tatcggttat cgttttactt ggaaagctga aattaaccat gctgacgatt
     1741 tttccaaagg gaaacaagaa gatttacgta taaaattatt taatattgat cacaatggag
     1801 aattaacacg agaagaaaaa tggcgcgcta aagatcgaat actaaatctt cccttaggca
     1861 ctcatgaagc tgattttaaa caacaacaac aaactgaaga aaaacaggca gaaaaacgag
     1921 aactttcaaa caaactcaaa caagatttac taggaaattt acaaaactta ttcgattaag
     1981 cttaaggagt atttatggca gataaaacaa tcaaagaact tgcggaagaa ttaggagtta
     2041 gtaaaacagc aattaacaaa aaagtttctg acaaaaatcg aaaactttgg tttgcaaaaa
     2101 acggaaacaa gtttgtgatt aacgaaaccg gtcaaaaagc tataaaatca atgtttttag
     2161 tcgataacgc aaacttaaat cgaaaactag ttggcgaaaa atcaaaaacc aaaaaccata
     2221 aaaacaccaa ccaagtttcc gaattaaaac tcctttcttt cctagaaaaa cagttaaatc
     2281 gaaaagatat acagctacaa gaaaaagata atcaaataaa acaaatgcaa aaattactcg
     2341 accagcaaca acaactcact ctacaagcga accaacaaat tgagaaccta caagaacagc
     2401 tccatttaac ttatacagaa gaaactccaa caaccgaatc aactgccttt tctgaaaaaa
     2461 aagcagacaa tacacaacca cttattgaaa aaaagtggtg gcaaatttgg aaatctaata
     2521 gaaccaatct ctaaaatttt ctagaagtta gttcggatag tagaggaaaa agttgtacta
     2581 cctgatgggg agatcactca aatttttatg agaaagaaaa aatagtttac aactgtaaac
     2641 aatcaatgtt tatagttgta aactatcaaa ctatatggtg acttattgct taaaataggt
     2701 tgaaatctca ctcaattttt ggtggaaaaa caaaataaat tctaatcgat tttgttgaaa
     2761 aagtcataaa aaaacaaatg tgattttaac aaaatttcaa aaaaatcgtt tgaaatttcg
     2821 tttatttttt ttgagaattt tgatcatcgt agggtcattg tagaatcatt atgagatcat
     2881 cgtgggatca tcgaataaag tatgggaaag gcttgcggtg cttggtatta agggcattct
     2941 ccgcctccca ggttcttgac cattttctct tataagtggg atttttacca cgttgagaga
     3001 aaatggtcaa gaacctggga ggcggagaaa acataccaag caccacaagc aaaaaaagtt
     3061 agagtaactt ccaaaaaaga aaaatttttt agaacaactt ttgaaaattt aattttgatt
     3121 tatatccgtt caatttaaac atttgataaa ctgaaggcta gtatttaaaa tgtgtataca
     3181 agtggtagcg ttaaaaaaag tggaggtata acctatgtta aaaaaaactg tgttggtact
     3241 gggttctatt tctctaggaa tattaggttt tagccccatg ctattatccg taattggaaa
     3301 aaacaattcc aacagagaac ttaaattgaa ttcaaattat tacaacagtc ctaacgaaga
     3361 attgtttgta aaagaaacgg ataaataata aaaaagactg ctcttccgag cagccagtta
     3421 aaaaataatg agtgtttatt cttgaaaaag caacgcgctt ataaagcgct accaaaaatt
     3481 tatcatattt tatgaaaaat aacaatccag taaaaaatga ctggtaaaaa ttcacaaaga
     3541 aacggaaaga agcaactgca gaaaatcaag ccttaaaaag agctttttac ctagaccaat
     3601 taaaaatcac aaagtgagcc cctgtatcgg ttgatacaag ggctacttgg catactatac
     3661 attatgtcta ctaaccttta aaataacacg ttttataaaa taaaccctct tgacaatatt
     3721 taattttctg attattctaa atatatacag aaagcgcttt cctaggattt tatatctaag
     3781 gaggaagtaa caatgaaaaa taaaattatt aaaattatta cttgtctatt tttgttcggt
     3841 atgattgttg gagcaagtac tccgttaaat ctgtttcctt ttagtgctag aggtataaaa
     3901 gtggaagctg caagttcacg atataatcat aatcatcgtg gatttacttg ctataaaatg
     3961 aactattatg ttactaataa aatgtgtaga gatttgaaaa aaaattataa taagctaaag
     4021 actcctgctc aaattgcatc ttttattcct attggtggag ttggaacatg ggttattaca
     4081 aatgctttta gtagtgctgt tgatgcaatg aatgtctttg taagagctgc aaatcaagga
     4141 aaaggtgtac aattaacata taatgctcac tttagcaata caacatcata tcaatacaat
     4201 gattacgctc gttatgtaat aaaataatca gaaagtgaaa ctatctcatg aataaaaaga
     4261 ttttagctgt aattatttca ataatagttt taatcactat gttcttcata tttaggctaa
     4321 ttttcaatat aagcttacaa aaaagtattc tttatctaat tcctatagca tttatcttag
     4381 caatttttag aagtatttac ggaaataaaa actctaaata gctaaaaata aatcatacta
     4441 aattttacaa aaagtggc
//
LOCUS       DANKEF010000124         4313 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000124 DANKEF010000000
VERSION     DANKEF010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.127"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..244)
                     /locus_tag="PWZ48_002837"
     CDS             complement(<1..244)
                     /locus_tag="PWZ48_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2262441.1"
                     /translation="MEDLGSKLRELRVKKGYTQEYVARKFNVTRQTISKWENNKTTPD
                     IETLKKICEFYSTNINSILNEDNSKNKKNNLLVLTAI"
     gene            377..751
                     /locus_tag="PWZ48_002838"
     CDS             377..751
                     /locus_tag="PWZ48_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HDL2262442.1"
                     /translation="MKKFLCLSALMGVLLTSGGIASATEALNLDVPEDHTAIYGGGME
                     ATEEGFSSSKLRYAAGGGDFNCGVNGFKVYANYYHARAKHSATAKNGRGGQVRSVQKA
                     GVRAYATCNATLTGNTGWWNVY"
     gene            805..2625
                     /locus_tag="PWZ48_002839"
                     /pseudo
     CDS             805..2625
                     /locus_tag="PWZ48_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2675..3847)
                     /locus_tag="PWZ48_002840"
     CDS             complement(2675..3847)
                     /locus_tag="PWZ48_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS256 family transposase"
                     /protein_id="HDL2262443.1"
                     /translation="MTQVHFTLKSEEIQSIIEYSVKDDVSKNILTTVFNQLMENQRTE
                     YIQAKEYERTENRQSQRNGYYERSFTTRVGTLELKVPRTRDGHFSPTVFERYQRNEKA
                     LMASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLEPMVNEWQNRLLSEKN
                     YPYLMTDVLYIKVREENRVLSKSCHIAIGITKDGDREIIGFMIQSGESEETWTTFFEY
                     LKERGLQGTELVISDAHKGLVSAIRKSFTNVSWQRCQVHFLRNIFTTIPKKNSKSFRE
                     AVKGIFKFTDINLAREAKNRLIHDYIDQPKYSKACASLDDGFEDAFQYTVQGNSHNRL
                     KSTNLIERLNQEVRRREKIIRIFPNQTSANRLIGAVLMDLHDEWIYSSRKYINFDK"
BASE COUNT         1477 a          666 c          634 g         1536 t
ORIGIN      
        1 taatagctgt caataccagt aaattatttt ttttattttt tgaattatct tcatttaaaa
       61 tagaatttat gttagtacta taaaattcac aaattttttt tagtgtctct atatctggtg
      121 ttgttttgtt attttcccac tttgaaatag tttgacgtgt aacgttaaat tttcttgcca
      181 catattcttg agtataccct tttttcactc ttaattctct tagcttagat cctaaatcct
      241 ccattttttc tcctatgtaa acgattattt acataaaatt ttatcataaa aatactaacg
      301 cgccaaaaaa gttctttatt ttataaaaca cactaaaata aaataattct agtaaaaaag
      361 ataggagaaa taagttatga aaaaattttt atgtctttct gctttaatgg gtgtcttatt
      421 aactagtgga ggaatagcta gtgccacaga agctttaaat ctagatgtac ccgaagacca
      481 taccgccatt tacggaggag gaatggaagc tacagaagaa ggattttctt ctagtaaatt
      541 gagatatgct gctggcggcg gggatttcaa ctgtggggtt aatggattta aagtttatgc
      601 aaattactac catgctagag ctaaacatag tgcaacagct aaaaatggac gaggcggtca
      661 agtacgatca gttcagaaag ctggagtaag agcttatgca acatgcaatg ccacacttac
      721 tggaaatact ggttggtgga atgtttatta aaaaagagag tttaaactct cttttttatt
      781 tacatttgaa aggaaattca aaatatgtta aaaaaaataa acgctgtttt atcacttttt
      841 gtctgcatta caatactatt tttttctact aatgttataa atggtataaa gacttctgaa
      901 gcgctatata aaaataccgc tgaaattcat atgcaatata aagatgatat ggactttaac
      961 agttatataa acgctttgta tgaaatatct acacaagaaa atattaatat ttctcaatat
     1021 agttttactg ctgttaacca actaactatt cttgctacaa accctaacgc taacaaacac
     1081 tggaaatttt ctaaaaaaaa tatattagca agaaacgatt ctaaagtcca ttatactaat
     1141 aacaaaaatt ctaagtacca attaaaactt ccaaataatt ttttaaatat aaatatttat
     1201 cctttttctc atgtggaaaa tattggatta agtgaaatat tatatgtaca aggaaactca
     1261 aacaaattaa ttcctgtact gaaaaaatat ggaacaccta ctatttccaa tatcaatcaa
     1321 tcagatactt ttcaaattaa tactaatatt gttcttgtta tctgctatct tagtatattt
     1381 attgtaatta ctacaattat atttgctttt tcaaaaatga aagaaatcac tctaaaaaaa
     1441 atgttaggct ataactcttt tgatactata aaagattcta ttaaagagtt aaacctctta
     1501 ttttgttctt tgattataac tttcatttta tctatttttt tgagcatata ttacagtaac
     1561 gaaaattttt tcctatcatt tcttctatgt agtatttttc attttataat tagtttttta
     1621 tttatcatag ttactctaag tatctatcat ttatctagta taaaaaatta tctaaaaaat
     1681 tcacgaccac ttaagttaaa tctttgtata ttaaatatct gtctattctt aagcatgatc
     1741 cttctgttaa tagcttctac aaaagtaata aatacccata atgaagccga aaataattct
     1801 cttaaatatt gggaaagaac tactaattta tacaaaacaa atattactaa tcaattaaat
     1861 agaaacaaca ccgtagaaga aaataactac ttgaaaaaag ctagtaaatt tgtttataaa
     1921 atacagaaga attataaaac ttttattatc gcgccataca attacgcaac tatccaagag
     1981 aataataaag aattttttat tggacaaaaa gtttatccaa attttgaaga ctatgttagt
     2041 caacctgctg gtgcaggaat atgtgttgat ttaaactatc taaaatatta caatcctatt
     2101 tcttttgaag aatctactga cttaaattta ataaactctg atccactaac tacttacatt
     2161 ctagtaccta aaaaatataa agaatatgca aaaatgattg aaaaaaatta tttagaagac
     2221 atacagttta gacttgagga ctctcctcct caagctcctt ataaaaatcc taatttgaaa
     2281 aatttaaaaa taaaactaat atttgttaat aataaccaaa agtattttag ttttaatacc
     2341 ttatatggaa aggcaaaaga taactataca ataactgatc ccatagttag agttattaat
     2401 ccagaaataa cagaatcatt attttgggga aatatattga ctagtgatgg tggattattt
     2461 tttgatcaga aacatacttc aaaccaaaac ttttttaatc aaattaatcc gtatataaaa
     2521 gaatttaacc tagaaaatat cataaatttt tcttattctg tttttcaaga aactggtgaa
     2581 ttattatctc aaaaaaaagt aaataaatta aatgcatatc tacaagtcaa gtccagactc
     2641 ctgtgtaaaa tgctatacaa tgtttttacc atttctactt atcaaaattg atgtattttc
     2701 ttgaagaata aatccattca tcatgtaggt ccataagaac ggctccaatt aagcgattgg
     2761 ctgatgtttg attggggaag atgcgaataa tcttttctct tctgcgtact tcttgattca
     2821 gtcgttcaat tagattggta ctctttagtc gattgtggga atttccttgt acggtatatt
     2881 gaaaggcgtc ttcgaatcca tcatccaatg atgcgcaagc ttttgaatat tttggttgat
     2941 cgatataatc atgaatcaat cgatttttag cctcacgcgc taagttaata tctgtgaact
     3001 taaaaattcc tttaacagct tctctgaaag attttgaatt ttttttagga atggtggtaa
     3061 agatatttct taggaagtga acttggcatc tttgccaact tacgttggtg aaggattttc
     3121 taatggcaga gactaatcct ttgtgcgcat cagaaataac gagttccgta ccttgtaaac
     3181 cgcgttcttt taggtattca aaaaatgttg tccaggtctc ttcgctttcg ccactttgaa
     3241 tcatgaagcc gataatttca cggtcgccat ctttggttat tccaatcgct atatgacagc
     3301 tttttgagag tactcgattt tcttctcgta cttttatata gagtacatcg gtcattaagt
     3361 aaggataatt tttttctgat aataaacgat tctgccactc gttaaccata ggttctagct
     3421 gttctgttaa gctagaaacg aaggacttag agacggattt accacaaagt tcttccacaa
     3481 tttttgatac tttacgagtt gaaacgcctg atacatacat ttccaacatt gaagccatga
     3541 gggctttttc gtttcgttga taacgttcaa acactgtggg tgaaaaatgg ccatcacgtg
     3601 ttctgggtac ttttaattct agcgtgccta cacgtgtcgt aaagctgcgc tcataatagc
     3661 catttcgttg actttgtcgg ttttctgttc gttcatattc ttttgcttga atatattctg
     3721 ttcgttgatt ttccattagt tgattaaata ccgttgttaa aatattttta gaaacgtcat
     3781 cctttacaga atattcaata atgctttgaa tctcttcgct tttcagtgta aaatgtactt
     3841 gggtcatgta aaagtcctcc tgggtatgtt tttgtcgtta aaaacattgt accgtaaaag
     3901 gactgttata tggccttttt acttttacac aattatacgg actttatcta tataaaggcg
     3961 ctgtcagagt tttttgactt agaacataca ttcattcaag agactttaaa caagctagca
     4021 gaacgcccaa aaacggacac acaactcgat ttgtttagct atgatacagg ctgaaaataa
     4081 aacccgcact atgccattac atttatatct atgatacgtg tttgtttttc tgtactgttt
     4141 cctgagtgat tagcagaaat ataaagatta aaattttaat tgattattag gaagagaagg
     4201 agaatgtatc ccgaaaactt tttagttggc ttggactgaa cgaagtgagg gaaaggctac
     4261 taaaacgtcg aggggagtgg gagcgaagcg cacacttgat cttttgaact ttt
//
LOCUS       DANKEF010000125         4153 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000125 DANKEF010000000
VERSION     DANKEF010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4153
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.128"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..106
                     /locus_tag="PWZ48_002841"
     CDS             <1..106
                     /locus_tag="PWZ48_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2262444.1"
                     /translation="ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(359..508)
                     /locus_tag="PWZ48_002842"
     CDS             complement(359..508)
                     /locus_tag="PWZ48_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262445.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            970..1182
                     /locus_tag="PWZ48_002843"
     CDS             970..1182
                     /locus_tag="PWZ48_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262446.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1460..3259)
                     /locus_tag="PWZ48_002844"
     CDS             complement(1460..3259)
                     /locus_tag="PWZ48_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDL2262447.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3383..3979)
                     /locus_tag="PWZ48_002845"
     CDS             complement(3383..3979)
                     /locus_tag="PWZ48_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2262448.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1490 a          728 c          707 g         1228 t
ORIGIN      
        1 cgcatctaaa cgaaataata ttcctagaaa atcactggat tatcgaacac ctttggaagt
       61 atttttgagt tacgtaagta ttgatgatct gtctaactta atttgacaat taagattata
      121 ttaaatatta tacagataat aacggaatat tatcatcgaa acagctaaca aaattgtcat
      181 aaaaaacgag atggttgata ttagaacttg tgtttttgtt taggtctaga gataaagaga
      241 gacaatttcc aaatatatgt aatgaataca aaaagacgtt tgtcgcggat taccgttaca
      301 aacgtctttt attgattttc cgagatctca tcagtcgcca tttggaatgg ttcactcctt
      361 atctagattc gtttaatgct cggactgctt gttcgattgt aataccaaac gcaacgttat
      421 tttcgttttg tgcatcaatc gccataaaac ggttagaagt tgtatcaaaa gctacgcggt
      481 attgcatatt tgtttttgta aacgtcataa aattcatctc ctttgtaatg taatttagtt
      541 cttgaaagtt tcctttaaac tttcgaactt ataccaagtt tagcatcgta cgttttttga
      601 tgcaattaaa aagcatttat cattttcctt atttattttt tcttaaatgg tttagctatt
      661 ttcttttaat ccgataagta gcccaatcat caaggttaca aatactattg cttgaatata
      721 aatcatatgc tcacctcttt ataaataact tagcaatata aagaaaaaaa cgcacgaaca
      781 acataggaat ataaacaaac gtttttgtca caacgtctaa gatcgtgtta aaaaatagtt
      841 atctaaaaag cagaaagata ctcaaaataa aaaataaaaa aatggataaa aacgttttca
      901 aatacaaggt aatttgttat tataaaataa agaaaaaaca aaataaaaat aaagaaaaga
      961 gggaatgaaa tggtaaaagc agaagtagga agatcttata tatgcaaacc gatcggagta
     1021 tctcatgaac taatcggctg tattgaacgt acatatatca acacggctgt cgtgtatgtt
     1081 gaaagttatg accaacgaga ctatcaactt atcaatgaaa gcaagtatcg catgctcgta
     1141 aaattctgtg atattagcgc acctgctgaa ttggtttctt aaacagtatt tttgagaatt
     1201 cgcaagaact cccttataat ataaataaag acagaaggga gcgagaagat atgccaaaaa
     1261 gaaaaagttc agggaaaatg ggaattattc ttgcagtgat ctctgcaatt cctgctattt
     1321 tagctattgt tcgtgcgatg aaggaatcaa gagaaagtta agcacacatt atgagcgtca
     1381 aacaaaaaac gcatatacct gcggtggcag gttatgcgtt ttttgttgta taaagacttt
     1441 tttcattaac gatttttgat tagccgactc ccaaaacaaa atttagcagt ttatctgaat
     1501 aagtgatttc ttcatctgtt tgtttcacac tttcatttag tagtcccatc tcatcggtta
     1561 tgataccatc cacaccataa aacatcatgc gactcatagc atctgaatcg ttgattgtcc
     1621 atacatatac ttcttttccg tcttggtggg cagaattaat gaagttacgg ttgatcgtac
     1681 tatattccat tgtcagaaaa tcagcaggcg tcacaggggg cccaacaata ttgaaaggta
     1741 agatgtagcc cacataaaag tttggttctg cttcttttaa atctgaaacg gtttggtacg
     1801 tcaaactttg caaaatatga tggttctcta agataacttt gcggtattta tggacaaatc
     1861 gttcgacaag gtcatccgaa tcttttttag aggtcttgat ctcaataagt aatttttggt
     1921 ttagcttatg tgctttttcc aaataagaat caaaagaaca aacaggggct tgcactccgt
     1981 tttcagtaac tgtaagattt gtcaattctt tcaatgtaag atcctgtggt gttccattta
     2041 cccctgttaa gtgacgaaga tttgcatcat gcatcactac gaaatgacca tcttttgttt
     2101 cctgaacatc catctctaca taatcaggat gatagtctct gcttgttttt tcaagtgctt
     2161 ctaaagtatt ttgcgcccca ttttgggtac tcacacctct gtgagagaca gtcactggtt
     2221 ttgtctgaac agctgaagtc agataatcca tattgtaaag acagacgcca ataccgaaaa
     2281 aaactgcaaa aagagtcaaa ccagtgtttt tcagcgcaga gaaacgcaag ttgggggctt
     2341 gtgggataaa ccacttaggg atctcaggca agaagccttc gtcatccatg aaatcaacaa
     2401 tgatataaaa aatccctacg gtagacatca cgatattgaa taataaaatc ccttgtaaaa
     2461 gcgtcatagc aaacacagcg ctgatcaatg catagtctgg gaaaaattgt tcaaccatac
     2521 tttgtgccaa aataacaacg atatatccta aagaagaaag gagtaaaatc gtaccgccaa
     2581 tcacaataaa ttggcccgtg atggccaaaa gacgcgattt ggtcaatgac cagctttccc
     2641 gaatggctgc tcgaaaagga cgatctcgta gaatcatttc cggtaaagca aaaatcagtc
     2701 gaatcccaat atagccaagg aaaatataaa cgagtaaaaa ggaactgacg atgatccaac
     2761 gattagcaaa aataaaatcc ataatgaaag cggggatttt tatttttgac agtagatcag
     2821 aattaaaact taatccgctg atcggtagaa tcaataaaaa ataagcaagg aaaaacaaaa
     2881 aagtaatggg tctcactttc ttcagctgaa gtatcgtcaa gtgtaagagt tgcttcaaag
     2941 agattggttc tttcttttta ataaaaaaaa cactcatcaa aagaaaagta aattcgaaat
     3001 aaactaacag taaaatcgtc atcaaaacta cgattaacga gagtagaacc ccaggatgtt
     3061 gagaaaaaat tgaaggaata ttgtcataag acaaatagtc aattgcccct cggtgtaaaa
     3121 taaaacgggt cgtactgctc aataagggta aacaaataaa taaaataaag ccatgcatca
     3181 gcaagacatc acggaaataa gcttgtgttc cttttaaaaa gtcccaagca ttacctaagc
     3241 tacgtttcaa ataattcatc ctatcacctg ctcttcacat tgtagcgcaa atctaatgaa
     3301 aaaggaaagg gtgcagtgtg ttttgcagac cctttcccag atttaacatt gtcaatcaat
     3361 gactaatttt acaaaataaa tattatccaa gaagaccact tagatattga cctccataaa
     3421 tcaaggccat actatataag aaaatcgaca gtggtttagc aatcaaaatg attagtgtat
     3481 atcgtttcac tgacatgttt gtcaatccag ccatcaaaca taacgcatcg tcaggagcaa
     3541 ctggaagaaa aatagctaga gcaaacagct tctcaaaacg gttttgatta tccagccagc
     3601 caatatattt gttatatgtc ttatcgctaa tcatggaaag tatgaatggc tttccataac
     3661 gacgccctag cagaaaaatg ataatcgaac caatgcagat tccaatatag ttatagataa
     3721 atccagcata agggccaaat atcaggacac cagctgcagt actgatccct ccaggaatga
     3781 ttggaataac tacttgcagg atttggatga agacaaaaat aatcggtcct aaaatgatgg
     3841 aatcacctac taatcctctt aacgcattca aatctttgaa aacacctaaa ttaataaaat
     3901 aaatcgtcag cgcgattgat aggcctaatc cgataatgga tatgaaattt atcagttttc
     3961 gagaggcagc aatattcatt attcatcaac tccttagagg ttctttgtta actatacttt
     4021 actagaaaag caactaactg cgccattttt tgatatgaca gaaaacaaac aattttgtaa
     4081 gttcatcata agtataaaag attgatctca aaaaaacatc caactaaaga cttatttaat
     4141 aacttgtttt ttt
//
LOCUS       DANKEF010000126         4146 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.129, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000126 DANKEF010000000
VERSION     DANKEF010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.129"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            1..291
                     /locus_tag="PWZ48_002846"
                     /pseudo
     CDS             1..291
                     /locus_tag="PWZ48_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            complement(984..1631)
                     /locus_tag="PWZ48_002847"
     CDS             complement(984..1631)
                     /locus_tag="PWZ48_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HDL2262449.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(1761..2699)
                     /locus_tag="PWZ48_002848"
     CDS             complement(1761..2699)
                     /locus_tag="PWZ48_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2262450.1"
                     /translation="MKKIDEKLAAEISLKKPAVSNSRLSNSRLSGQTLGKSQPALSFD
                     AMTIVGNLNKTNAKKLSDFMSVEPQIRLWDILQTKFKAKALQEKVYIEYDKVKADTWD
                     RRNMRVEFNPNKLTHEEMIWLKQNIIDYMEDDGFTRLDLAFDFEDDLSDYYAMTDKAV
                     KKTVFYGRNGKPETKYFGVRDSDRFIRIYNKKQERKDNADVEVVSEHLWRVEVELKRD
                     MVDYWNDCFNDLHILKPAWATLEKINEQAMVYTLLHEESMWGKLSKNTKTKFKKLIRE
                     ISPIDLTELMKSTLKANEKQLQKQIDFWQREFRFWK"
     gene            3190..4146
                     /locus_tag="PWZ48_002849"
                     /pseudo
     CDS             3190..4146
                     /locus_tag="PWZ48_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
BASE COUNT         1465 a          696 c          606 g         1379 t
ORIGIN      
        1 atagagaatt ttaaaaaaga accttttttc gaggtggaag ctagtataaa agtaaaccaa
       61 gggtcgttta agggcgttct aagccccaca cagcgtttta aaacccaaga ggagctttta
      121 gcttttgttt cttctaaaca agctaaaata ggcaatcaag aggggataat tgctgatgtt
      181 caaaccaaag agaagaaaac gaatagtccg agtttgtttt ctttaagtag tttgcaatca
      241 aaagtcaatc agctttataa agcgacagcg agccaaactt taaaagctat ttctttttta
      301 ataacttaaa aataaactta atgtaacagc aagcacagtc aaggtataca cctttgacaa
      361 aaaatagcac attctctatc gaaaattttt gcttattttt taaattattt tgggaaattt
      421 tcccaatccc tttttctaac tcaaaaaata taatcactca aaatttaaaa gggcgcactt
      481 atacatcatt ttaaaaaatt gatgtaacgt gctaagttca aaacaaaggg cgcacttata
      541 cacgattttc aatcttgtat atttctaacg aaaagcgtgc gccaaaaaac ccccttcgtc
      601 aattttgaca gggggctttt tgatgtaaaa atttctatcg aaatttaaaa attcgcttca
      661 ctcatgttat aaagacttaa aataaaataa ctctttaaaa tcttttgcta gttgttcttc
      721 aatatttttt attcggtgca tcttccaagt aaagtataac acactagact tatttactac
      781 gtttcataag tcattaatgc gtgtgctctg cgaggctagt ttttgtgcaa gcacaaaaaa
      841 tggactgaat aaatcagtcc ataagttcaa aaccaaattc aaaatcaaaa ccacaagcaa
      901 ccaaaaaatg tggttgttat acgttcataa attttatgat cacttacgtg tataaaatta
      961 aattcacttt caaaatctaa aaactaaatc caatcatcta ccctatgaat tatatcttga
     1021 aattcattca taaatagtga agcatggtaa ccatcacata cagaatgatg aagttgcaga
     1081 gcaactggta tataaatttt attattctca ctataaaatt tacctatcgt aataataggc
     1141 aataaaaagc tgctattgtt accaatattt aaattaaatg aactaaaatc aatccaagga
     1201 atcattgaaa tcggtatggt gttttcaggt atcggttttt taggaaacat ttcttcttta
     1261 tctttatatt caagcaagtc atttttataa ttattataaa aagaaatgaa gtttttatca
     1321 gattcagtcc aaatgttagt aaatttttca gtttgcttat taaaaactgt atacaaagga
     1381 tttaacttat cccaataacc taatttattc tcactattaa ttcctgttct aaacacttta
     1441 tttttattta caacttccat aattgcataa attaaagagg gataaatttc atatcctttc
     1501 ttttttatca tatctttaaa caaagtaata tcaatttctt tagtaatgct ataagtagtt
     1561 tgctgattaa aatagtgttc aaaatattct tttctatccc aattttctaa ttcaataata
     1621 ttaaaagtca tatataactt cctcctaaat tttaaatttt tatatttagg aggaataatc
     1681 ctctgatttt ttcatacgtt atgtcacctc gtaaatatta attatactga attagcaatt
     1741 tttatcaaat aaaacttatt ttacttccaa aacctaaatt cacgttgcca aaaatcaatc
     1801 tgcttttgca attgtttttc gttcgctttt aaagtcgatt tcattaattc cgttaaatca
     1861 attggagata tttctctaat caatttttta aatttagtct tagtattctt acttagcttt
     1921 ccccacatac tttcttcatg caacaaagta taaaccatag cttgctcatt aattttttct
     1981 aaagtagccc acgcaggttt caagatgtgt aaatcattaa aacaatcatt ccagtaatca
     2041 accatatctc tttttaattc aacttctaca cgccataaat gttcagacac aacttcaaca
     2101 tctgcgttat ctttacgttc ttgtttttta ttataaattc taataaatct atcactatca
     2161 cggacaccaa aatattttgt ttctggcttg ccattacgac cataaaaaac agttttctta
     2221 actgctttat cagtcattgc atagtaatcg ctcaaatcat cttcaaaatc aaaagctaag
     2281 tctaatcttg taaaaccgtc atcttccatg tagtcgataa tattttgttt taaccaaatc
     2341 atttcttcat gtgtgagttt attgggatta aattcaacac gcatattacg tctatcccaa
     2401 gtatctgctt ttactttgtc atattcgata taaacttttt cttgaagtgc cttagcttta
     2461 aactttgttt gaagtatatc ccaaagtcgt atttgtggct ctacactcat aaagtcagat
     2521 agctttttag cattagtttt gttcaaattt ccaacgattg tcatggcatc aaaacttaat
     2581 gcgggttgag attttcccaa agtttgacca cttaaccggc tattacttaa ccggctatta
     2641 gagacggccg gtttttttag tgagatttct gcagctaatt tttcatcaat tttcttcatt
     2701 ttttatcgct cctaaaataa tttttggaac gaaaaaagga cgagtggaat cgcaaaataa
     2761 atttcgcctg tatccattcg tccattttgt tttgtattca aaatcaaaaa ttgatatact
     2821 aatcatcgaa tgaataacaa gtgtggtttg ttattccggg cgagaacgtt ggtagcgttg
     2881 ctcgtctttt tttatgtctt tttttcgtta aaactatttt aaaactaaat atatcttaaa
     2941 gcaaataaat tcaaggggct aaaagcgccc cttaaaaacc ccttgcctct gaaaagtttt
     3001 gtgtttcctt tccgtcggtt tcaaaagcta atttactcaa aaaaatagat caagggtaaa
     3061 tgcactcact gcgttcgccc ttgagctatt ttatttcgca aattttaacg catttgccga
     3121 cgaaaagata aaacacataa aaatatacgc agatatattt aaaacgtctt agaacgcaaa
     3181 attttacgtt taaaagctat gcaaggactg tatgaagcaa aattattgag ttatccaaga
     3241 acagatacac catttattac agagaacgaa tttgcttatt taaaagcgaa ttttggcaaa
     3301 tatagcggtt ttttaggact tgatcttgaa atggttcaaa cagagcctag aaagcgttat
     3361 gtggacggta gtaaggtaca ggaacaccac gccattatcc caacaaaaca agtacctacc
     3421 gaatctgcat tagcgaaaat ggacgattta caacgaaaaa tttatgcttt agtcgttaaa
     3481 acgaccgttg ccatgttttt acctgattac ttgtatgaag aaaccaagat acaaacgaaa
     3541 gtagctgatt tgctgtttca atcgatcgga aagacaccaa agcaagaagg gtggaaaatt
     3601 cttttcaaac aacaaaccaa agaagaaaaa gaggacgttc aaacgttacc actcgttatc
     3661 attggcgaac gtgccgaagt tggtgttaag agtgttgaaa aagaaacgca accgccaaaa
     3721 gcttttacag agggtacatt attaactgct atgaaaacgg cgaataaaac ggttgatgat
     3781 gaagaagcaa tcaagatttt acaagaagtt gaggggattg gaacagaagc gacaagagca
     3841 agtattattg aagcgttaaa acaaaaagaa tatatccaag tgattaagaa taagcttgtt
     3901 gtaactgaaa aaggaaaatt attgtgccag gcagttgaaa gtcagcacct tttaacgagt
     3961 gctgaaatga cggctaaatg ggaaacgtat ttaaaaaaaa tcggtaaaag agaaggcaat
     4021 caagagaact ttattacgaa tatcaaaaaa ttcattgttc atttactgga agctgtacct
     4081 aacgatatag aaaaactaaa tttttctgat taccaggaac agaaagaaaa agaagcagaa
     4141 aaaagt
//
LOCUS       DANKEF010000127         4116 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000127 DANKEF010000000
VERSION     DANKEF010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.131"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..85)
                     /locus_tag="PWZ48_002850"
     CDS             complement(<1..85)
                     /locus_tag="PWZ48_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262451.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            155..316
                     /locus_tag="PWZ48_002851"
     CDS             155..316
                     /locus_tag="PWZ48_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDL2262452.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="PWZ48_002852"
     CDS             335..1612
                     /locus_tag="PWZ48_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2262453.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="PWZ48_002853"
     CDS             1622..2278
                     /locus_tag="PWZ48_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2262454.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="PWZ48_002854"
     CDS             2278..3654
                     /locus_tag="PWZ48_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDL2262455.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..>4116
                     /locus_tag="PWZ48_002855"
     CDS             3784..>4116
                     /locus_tag="PWZ48_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2262456.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1629 a          455 c          727 g         1305 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DANKEF010000128         4075 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000128 DANKEF010000000
VERSION     DANKEF010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.132"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..221
                     /locus_tag="PWZ48_002856"
     CDS             <1..221
                     /locus_tag="PWZ48_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2262457.1"
                     /translation="CINEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLFECYDMTE
                     KLYNEVNEIVSEINNFLNEESSDERKQI"
     gene            202..936
                     /locus_tag="PWZ48_002857"
     CDS             202..936
                     /locus_tag="PWZ48_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2262458.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            969..1877
                     /locus_tag="PWZ48_002858"
     CDS             969..1877
                     /locus_tag="PWZ48_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2262459.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1874..2416
                     /gene="sat4"
                     /locus_tag="PWZ48_002859"
     CDS             1874..2416
                     /gene="sat4"
                     /locus_tag="PWZ48_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL2262460.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2509..3303
                     /locus_tag="PWZ48_002860"
                     /note="aph(3')-IIIa"
     CDS             2509..3303
                     /locus_tag="PWZ48_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2262461.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            3579..>4075
                     /locus_tag="PWZ48_002861"
     CDS             3579..>4075
                     /locus_tag="PWZ48_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2262462.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEP"
BASE COUNT         1398 a          621 c          935 g         1121 t
ORIGIN      
        1 attgtatcaa cgaaaagaaa gctataaaac tgcttgaaac ttttgaacat aaacctgaaa
       61 aatataccga gaaggtaaat catatttttg aagtactcgg tatctcactt tttgaatgct
      121 acgacatgac cgagaagctt tataatgaag tgaatgaaat tgtatcggag ataaataact
      181 ttttaaacga ggagagttca gatgaaagaa aacaaatatg atgataatat attttttcaa
      241 aaatacagtc aaatgagtcg ctcgcagaaa ggactggctg gtgcgggaga atgggagact
      301 ttgaaaaaga tgctacctga ttttaagggt aagcgtgtgc ttgatttagg atgcggctat
      361 ggatggcact gtatatatgc gatggaaaac ggtgcttcct ctgtagtagg tgttgatatt
      421 tctcataaaa tgctcgaagt agcaaaagga aaaacccatt ttccacagat tgaatatgaa
      481 tgctgtgcca tagaagatgt ggatttccca gaggagagct ttgatgtaat actaagttcg
      541 cttgcgtttc attatgtagc agactatgag aatttaataa aaaagatata taggatgctg
      601 aaggctggtg gcaatttagt ttttacagtt gaacatcctg tttttactgc tcatggaaca
      661 caagactggt attataacga aaaaggagaa atactgcatt tcccggtgga caattattat
      721 tatgagggca aacggacagc tatgtttttg gaagaaaagg ttacaaaata tcatagaaca
      781 ctgaccacat atctaaatac actgctttca aatagtttta taataaatca gattgtggag
      841 ccacagccgc cagagaacat gatggatatt ccggggatgg cggatgaaat gcgacgccca
      901 atgatgctga ttgtatcggc aaaaaagaag atgtaataat atagaaaaaa taaacgagga
      961 gtatgtaaat gagatcagaa aaagaaatga tggatttagt actttcttta gcagaacagg
     1021 atgaacgtat tcgaattgtg acccttgagg ggtcacgcgc aaatattaat atacctaaag
     1081 atgaatttca ggattatgat attacatatt ttgtaagtga tatagaaccg tttatatcta
     1141 atgatgactg gcttaatcaa tttgggaata taataatgat gcaaaagccg gaggatatgg
     1201 aattattccc acctgaagaa aagggatttt cctatcttat gctatttgat gattacaata
     1261 aaattgatct taccttattg cccttggaag agttagataa ttacctaaag ggcgataaat
     1321 taataaaggt tctaattgat aaagattgta gaattaaaag ggacatagtt ccgactgata
     1381 tagattatca tgtaagaaag ccaagcgcaa gggagtatga tgattgctgc aatgaatttt
     1441 ggaatgtaac accttatgtt attaaaggat tgtgccgtaa ggaaatttta tttgctattg
     1501 atcattttaa tcagattgtt cgccatgagc tgctgagaat gatatcatgg aaggtcggca
     1561 tcgaaacagg ctttaaatta agtgtaggca agaactataa gtttattgaa aggtatatat
     1621 ccgaggattt gtgggagaaa cttttgtcca cctaccggat ggattcctat gaaaacatat
     1681 gggaagcatt atttctatgc catcaattgt tcagggcggt atccggtgag gtggcggaaa
     1741 ggcttcatta tgcctatccg gagtatgata ggaatataac aaaatatacc agggacatgt
     1801 ataaaaaata cactggtaaa accggctgcc tggatagcac atatgccgct gatatagaag
     1861 agaggcggga acagtgatta cagaaatgaa agcagggcac ctgaaagata tcgataaacc
     1921 cagcgaacca tttgaggtga taggtaagat tataccgagg tatgaaaacg agaattggac
     1981 ctttacagaa ttactctatg aagcgccata tttaaaaagc taccaagacg aagaggatga
     2041 agaggatgag gaggcagatt gccttgaata tattgacaat actgataaga taatatatct
     2101 ttactaccaa gacgataaat gcgtcggaaa agttaaactg cgaaaaaatt ggaaccggta
     2161 cgcttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc
     2221 gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga
     2281 aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg
     2341 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
     2401 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc
     2461 ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg
     2521 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
     2581 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
     2641 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
     2701 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
     2761 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
     2821 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
     2881 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
     2941 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca
     3001 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
     3061 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
     3121 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
     3181 gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
     3241 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
     3301 tagtacctag atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct
     3361 tttctgaagt acatccgcaa ctgtccatac tctgatgttt tatatctttt ctaaaagttc
     3421 gctagataga gttctatatt agagatgtaa agagttttgg tgaagaggtt tgtgagtatg
     3481 gcttttataa ccagggtgca atgagaagca ctaacagcag ctagcttaag aacgcatagg
     3541 atatactttt tatggaagtc acagaggagg aataaaattt gaacaggatt aacagagtaa
     3601 cctctattct tattcagctg caatcaaaaa aaataattcc tgccaaagaa attgcacagc
     3661 gatttaatat aagcttaagg acagtttaca gagatatccg gacacttgaa gaagccggaa
     3721 taccaattgg atctgaggcc ggaaaaggat attttcttgt agagggcttc ctacttccgc
     3781 cggtaatgtt tacagcggcg gaagtgggtg cattgattac agcagggaaa tttttaaatt
     3841 gccatggaga tgaatcattt ataaaggatt ttgattcagc tatgtataaa atcaaatcta
     3901 ttttgaagca tggtgaaaaa aactatgcac aggagttgga gaacagtatt aacgtgtaca
     3961 gcacatctgg acagaaaaat acattggcgg ataacgttat tgcagcaatt cagactgcaa
     4021 tctgcaataa aagggtaata tcaattcaat atcctgcatc aggagggcaa gaacc
//
LOCUS       DANKEF010000129         4066 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000129 DANKEF010000000
VERSION     DANKEF010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.133"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            86..1435
                     /locus_tag="PWZ48_002862"
     CDS             86..1435
                     /locus_tag="PWZ48_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2262463.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1563..2153)
                     /locus_tag="PWZ48_002863"
     CDS             complement(1563..2153)
                     /locus_tag="PWZ48_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HDL2262464.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2300..3652
                     /gene="brnQ"
                     /locus_tag="PWZ48_002864"
     CDS             2300..3652
                     /gene="brnQ"
                     /locus_tag="PWZ48_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HDL2262465.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 aaaattttgg aatcttagtt gtgtttttca gaaaactctc ttaatatgga agtaattaaa
       61 cttttttagt taggatgatt taaaaatgaa tgaagagcaa gaactgcagc gtggactgaa
      121 aaatagacat gtccaactga tttctattgg cggagcgatt ggaacggggt tgtttttagg
      181 ttccggaaag tctatccagc tagctggccc ttcgatattg ctcgcctatc ttattactgg
      241 atgcatctgt tttttcatta tgcgtgcgtt aggcgaactt ctgttatcaa acaccaataa
      301 ccattctttt ctggattttg ttgcagaata tcttggcaaa aaagcggctt tcatcactgg
      361 ctggacgtat tggttctgct gggtatcgat tgcaatggcc gatttaacag ccatcggaat
      421 gtatgtacgc tactgggcgc ctggtgttcc tcagtggctt cctgaactga ttgcattagg
      481 tcttcttctt tgcttgaata tggtggctgt ctcattgttt ggtgagttag agttttggtt
      541 tgcattgatc aaagttgtag cgatcattgc ctttatcctc gtaggagctt atatgatcct
      601 cacccactat acgacagata ttggcacagc cagtatcacg aatctatggt ctcatggcgg
      661 attctttcca actggtgcaa aaggatttat cttagctttc caaatggtaa cctttgcttt
      721 tgctgggata gaattagttg gattagtcgc tggagaaaca gaaaatcctg aaaaagtgtt
      781 gccggaagct atcaataata ttcctatcag gatcatctta ttttacttag gatctctttt
      841 tgtcattatg gcaatctatc catggaacag cttgaatcca gataacagtc catttgtaga
      901 agtattttcg gaaattggga tcacgattgc tgcttccttg atcaatttag tcgtattaag
      961 tgcagcagct tctgcctgca acagtgcaat atatagtaca ggacgtatgc ttcgttcttt
     1021 agcgcaagaa ggcagtgccc ctaaaaaatt caaaaagtta acaacacatc atgtgccagg
     1081 gaatgctttg acattttcta ctatcgtcat tttcatttct gttatcttga attacgtgat
     1141 gcctagtgaa gtattcacac tagtaagcag tattgccact acttgtttct tgtttatctg
     1201 gagtatgctt gtctacacgc acatgaaata tcgtaaaagt atcctcggca aaaaagctca
     1261 ttcatttaaa atgccgttgt acccatttag taattatctg gtatttcttt acatggcctt
     1321 tgtgtgtgtc gtcttgttcc taggaaaaga tacgagaatc gctttattac taacacctgt
     1381 gtggttcttg cttctattac taatctatca tatgaaatat gaacgtaaga agtaagaaag
     1441 tagttgtgac agaagtggtc agcttcgaga aataagaagg aatttacgaa aattgcttct
     1501 taaatttttg tgaaatttcg gcttatttct tggagataaa agcttctgtc ccagcctctt
     1561 ttctatttcc ccggaaacaa ccgctgataa taggcatctg cttttttata ggaaaaaatc
     1621 caaataccag atacaaaaaa acgtccgaat aacccgatca acggtacaaa aaagacaccg
     1681 gcaatcaagc ataagttaat catcatggag ataagacttc gacggtcctt tgacaaaact
     1741 ctcacgacat gggaatctgg tttatttgct ttcatgattt gcctcatcaa gataccaaaa
     1801 gaaaaactcc acatcgtgta tactaagaca taagccatct caggccaaaa agcttcaggg
     1861 ttacgcccga cccaactagt aacagaagga cataaagtca gccaaaagag caaaaagata
     1921 ttcgaccaga ccactcggta attaattcgt tctgccatct gaaatatacg atgatggttc
     1981 acccagatca ccgcaacaaa tataaagctg atggtataag ctaataatgt atttcggatg
     2041 acccataaag aatgaaagct agatgtatta gggatcttga tctctaatac aagaactctc
     2101 aaaacaatgg ctaccactcc atcagtaaat gcttctaatc tcgactttgt cacgatgatt
     2161 cctcctcaga tgattcagcg cttttatttt atcacttacg ttattaaata accatcgttt
     2221 tatataggga attttctgaa aattgttgca tatttttcga aattttgtta cttttaaaaa
     2281 aaccgtaaag gagttatgta tgacaaaaaa attatcattt aaagactatc tttttatagg
     2341 ctccatgctt ttcggtctat tttttggtgc tggaaatctt atctttcccg ttcatttggg
     2401 tcaagaagca ggcgctgcta ccttttgggc gaatctaggt ttcttggtta ccggtatcgg
     2461 tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa agctcaggtg tatatgaatt
     2521 agcaaaacgg ataaatcaaa catatgcgat ggtttttacc atcttgcttt acttagttat
     2581 cggaccgttt tttgctttac ctcgccttgc aaccacatct ttcgagattg gtttagcacc
     2641 atttatcgat cctagcagac agacgctata tctagcaatt ttcagtattg tattttttgt
     2701 gctggcttgg tggttttcaa gaaaaccgac aaagatatta gattatgttg gaaagttcct
     2761 gaatcctgca tttttggttt tattaggtgc tttgcttctc cttgcttttt tgaatccact
     2821 aggttctgta aatcaagcgc ctattcagcc aaattatcaa gagcatgctt ttttcacagg
     2881 gtttactcaa ggatataaca cattagatgc gcttgctgct ttagcatttg ggatcatcat
     2941 tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct gaaatcgcta aagacacgat
     3001 caaatctggt gcgatcagca ttatattgat gggaatcatt tatactttgc tcgcttattt
     3061 aggtgctatg agcttaggta gctttgctct aagtgaaaat ggcgggatca cgctggctca
     3121 gattgctgat cattatttag gtacttatgg cagtatctta ttagcattta tcgttatatt
     3181 agcttgcttg aagaccggta ttggtttgat tacagctttt gcagaaacat tcacagattt
     3241 gtttccgcaa agaaattacg cattctttgt tgcacttgct agtatccttc cttgtttagc
     3301 ggcaaacgta ggcttgacga atatcattca attttcgttg cctgttttga tgttcatcta
     3361 tccattagca atgacactga ttttactagt gttagttggt cctttgttta agcagcgtac
     3421 ggctgtttat cgaatgacta cttattttac attgattgct tcgatttttg atgggttgaa
     3481 cgcttgccca gaaactatca aacaaacacc tattgttcaa aatcttttat atgttgcgga
     3541 aagctacttg ccgtttttca aattggggat ggggtggatc gtaccagcag ttatcggttt
     3601 tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa gtagcagatt gataaaagag
     3661 gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa ttttgaaaaa tggcttctca
     3721 catttttgca taaatttgac ttaataccga gaaactgact tttgaacacc gtttattgag
     3781 aggtcgtgaa aaagccgctt agtttcaagt attaagaaat attttgtgaa aatggcttct
     3841 cacatttttg cataaatttg acttaatacc gagaaactgg cttttgaaca ccgtttattg
     3901 agcgtctata aactgacctg agtattagag aaacaaaacg gcaaagtagc tccatatact
     3961 ttgccgtttt gttttgaact aataccgaag agaagctgat caaacaacat ttcttttata
     4021 atatagattt tagacatata tatgatataa aaatagtttt cgcaaa
//
LOCUS       DANKEF010000130         4059 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000130 DANKEF010000000
VERSION     DANKEF010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4059
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.134"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..561)
                     /locus_tag="PWZ48_002865"
     CDS             complement(<1..561)
                     /locus_tag="PWZ48_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDL2262466.1"
                     /translation="MKKTIMVTGGCGMIGSNLVKRLVKEGCWDVYVADNLWRGKLEYL
                     NDEDGHPVIDLDTHFFNADLTDYEQAKKVIGTTEYVVHLADVVAGIDYVFKNQGELFR
                     INNLINSNVFDCCRKAGKEKIKGVIYVGTVCSFPLTRQNTLNPEPLREEELFPALPES
                     AYGWSKLMGQLEMGYLEKETGIPCCIL"
     gene            complement(580..1233)
                     /locus_tag="PWZ48_002866"
     CDS             complement(580..1233)
                     /locus_tag="PWZ48_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2262467.1"
                     /translation="MLAEKLNKHIDLLIKRYPTLETIKQDIIDGYLIMEECYLNGGKL
                     LVAGNGGSAADSEHIAGELMKRFKTPRPVSKEFADKLVAIDPVRGPELAKNLECGLTA
                     IPLVAHEALSTAYINDVDGLGVFAQQLFGFGRSGDVFLGISTSGNSKNIMSATVVARA
                     LGIKVIGLTGAKGGELATVADVCVKAPEVETYMIQEYHLPIYHCWCLMLEDKFFGNE"
     gene            complement(1243..2253)
                     /locus_tag="PWZ48_002867"
     CDS             complement(1243..2253)
                     /locus_tag="PWZ48_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HDL2262468.1"
                     /translation="MIITKTPFRMSFFGGGTDMEDYFKENGGAVLSTTFDKYCYVNVR
                     HLPRFFDYSAELSYSRTERVTSIEDIEHPAIRNAMKMLDMHEIRLTYEADLPARSGLG
                     TSSSFAVGMLNAFYALEGKYADKKKLADEAIYLERVLCNEAGGWQDQIAASYGGFNRI
                     NFNVDGYEVLPVIISPERKKQLNNNLMMFFTGFTRFSSDVQKANNVSGTEDKRVRLKK
                     MYELVDEAEAILTDKNRNLDDFGRQLDVTWRLKKGTGGAISTGSIDELYEKGMAAGAL
                     GGKLLGAGGGGFLVFYVQPEKQDAVRWAMRDLMYIPFEFENGGTRVIHYTPETYIEKQ
                     NG"
     gene            complement(2272..3522)
                     /locus_tag="PWZ48_002868"
     CDS             complement(2272..3522)
                     /locus_tag="PWZ48_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HDL2262469.1"
                     /translation="MKKQYNIAVAGTGYVGLSIATLLAQHHKVTAVDIIPEKVELINN
                     RKSPIQDDYIEKYLAEKELNLTATLDAKAAYQSADFVVIAAPTNYNSRTQHFDTSAVE
                     AVIRLVMEVNPDAIMVIKSTIPVGYTASVRVKTGSKNIIFSPEFLRESKALYDNLYPS
                     RIIVGTDMDDERLVEDAHTFATLLQEGAIKENVDTLFMGFTEAEAVKLFANTYLALRV
                     SYFNELDTYAEMKGLNTQQIINGVCLDPRIGTHYNNPSFGYGGYCLPKDTKQLLANYA
                     DVPENLIEAIVESNRTRKDFIADRVLQMAGYYNYAEANQYDAGKEKPVVIGVYRLTMK
                     SNSDNFRQSSIQGVMKRVKAKGATVVIYEPTLKDGETFFGSKVVNDLEQFKAMCDSVI
                     ANRYDACLDDIKEKVYTRDIFQRD"
BASE COUNT         1204 a         1008 c          674 g         1173 t
ORIGIN      
        1 caggatacag cacggaattc cggtttcttt ttccaaatac cccatctcca actggcccat
       61 cagtttgctc cagccataag cagactcagg aagagcaggg aacaactcct cttcacggag
      121 aggctcagga ttcaaagtat tctgtctggt cagagggaag gagcatacgg taccaacata
      181 gataacaccc ttgattttct ccttgccagc cttacggcaa caatcaaaaa cattagaatt
      241 gatcagatta ttgattcgga acaattcgcc ctggttctta aacacatagt caatgccagc
      301 cacaacatca gccagatgaa ctacatactc agtcgtacca ataacctttt tagcctgctc
      361 ataatcagtc agatcagcat tgaagaaatg agtatccagg tcgatgacag gatgcccatc
      421 ttcgtcgttc aggtactcca actttcctct ccacagatta tcagctacat aaacatccca
      481 gcagccttcc ttcaccagtc tcttaacaag attggagcca atcataccgc aaccaccggt
      541 aaccataatt gtctttttca tttaacattc ctccaaattt tattcgtttc caaagaattt
      601 atcttccagc atcaaacacc aacaatgata tatcggcaag tgatactctt gaatcatgta
      661 tgtttcaact tcaggagcct taacacaaac atctgctacc gtggcaagtt ccccaccctt
      721 tgcgcccgtc aagccaataa cttttatacc gagagcccta gcaacaacag ttgcgctcat
      781 aatattcttg ctatttccgg acgtcgaaat accaaggaat acgtcaccag atctaccaaa
      841 gccaaacagc tgctgtgcga agacacccaa accatcgaca tcattaatat aagcagtaga
      901 caatgcttcg tgtgcgacca aaggaattgc cgttaaacca cactcgagat ttttggctaa
      961 ttccggacct cttacagggt caattgcaac taacttatcg gcaaattcct tacttacagg
     1021 acgtggcgtc ttaaaccgct tcatcaactc gccagcaata tgttccgagt cagcggctga
     1081 tcctccgttt cctgccacaa gaagtttacc accattcaaa tagcattcct ccataattag
     1141 gtatccatcg ataatgtcct gtttaatcgt ctcaagtgta ggatatcgtt taatcaataa
     1201 gtcaatatgt ttattaagtt tttccgctaa cattgttact ccttatccat tctgtttttc
     1261 aatataagtt tccggcgtgt agtgaataac tctagttcca ccattttcaa attcaaacgg
     1321 aatatacatc aaatcacgca tagcccaacg aactgcatcc tgtttctcag gttgtacata
     1381 gaaaaccaag aaacctccac cacctgcacc gagcaacttt ccgcctaaag cacctgcagc
     1441 catacccttt tcatatagtt catcaatgct tcctgtacta attgctccac cagtaccttt
     1501 tttcaatctc caagtaacat caagctgtct accgaaatca tctaaatttc tgttcttatc
     1561 agtaagaatt gcctcagctt catctacaag ctcatacatc tttttcagtc ttactctctt
     1621 atcctctgtg ccactgacgt tgttagcctt ttgaacatca gaagaaaatc tagtgaatcc
     1681 ggtaaagaac atcatcagat tgttgttaag ctgcttttta cgttcaggcg aaataatgac
     1741 aggcagcacc tcataaccat ccacattgaa attaattcga ttgaacccac cataagatgc
     1801 ggcaatctga tcctgccatc caccggcttc attacacaga actctttcaa gataaatggc
     1861 ttcatcagca agtttcttct tgtcagcata cttaccctcc agtgcataaa aagcattcaa
     1921 cataccaact gcaaaagaac tggatgtacc cagaccagag cgagcaggta aatcagcttc
     1981 gtatgtaagc cgaatttcat gcatatcgag cattttcatc gcattacgaa tcgcaggatg
     2041 ctcaatatct tcaatgctag ttactctctc agtacgagaa taagataact cagcgctgta
     2101 atcaaaaaat ctaggcagat gtctcacatt cacataacag tacttatcaa aagtagtaga
     2161 aagaactgca ccgccattct ctttgaaata atcctccata tccgttccac cgccaaaaaa
     2221 agacatacga aacggtgttt ttgtaataat cataaatact ctctcctaat cttagtctct
     2281 ctggaagata tcacgggtat atactttttc cttaatgtca tcaaggcaag catcgtagcg
     2341 atttgctata acagaatcac acattgcctt aaactgttcc aaatcattca caaccttact
     2401 gccaaagaaa gtctctccat ctttcaaggt cggctcataa atcacaacag ttgcaccttt
     2461 agcttttaca cgcttcataa cgccctggat agaactctgg cggaagttat cagaattgga
     2521 tttcatggtc aaacggtaaa caccaatcac aacaggcttt tctttgccag catcatactg
     2581 atttgcttct gcgtaattgt aataaccagc catctgaagt acacgatccg caataaagtc
     2641 cttacgagta cggttagatt caacgattgc ctcgatcaga ttttcaggaa catctgcgta
     2701 gttggccaga agctgcttgg tatccttcgg caggcaatag ccaccgtaac caaaggaagg
     2761 gttgttgtaa tgagtaccaa tacgaggatc aaggcaaact ccattaatga tctgctgagt
     2821 gttcaagcct ttcatctctg cataagtgtc cagctcattg aagtagctga cacgaagagc
     2881 cagatatgta ttggcaaaca gcttaacagc ttctgcttca gtaaagccca tgaacaatgt
     2941 gtccacattc tctttgattg cgccttcctg tagcagtgta gcaaaagtgt gtgcatcctc
     3001 aaccaaacgc tcatcatcca tatcggtacc cacaataata cgcgaaggat acagattgtc
     3061 atacagtgcc ttactttcac gcaggaactc agggctgaaa ataatattct tgctaccagt
     3121 ctttacacga acagaagcgg tgtatccaac aggaatggtg gacttaataa ccataatggc
     3181 atcaggattt acttccatga ccagcctaat aacagcctca acagcggatg tgtcaaaatg
     3241 ttgagtgcgg ctattgtagt tagtaggagc cgcaataaca acaaaatccg cagactggta
     3301 tgctgccttt gcatccaatg ttgcggtcag attcagttct ttctcagcca agtacttttc
     3361 aatataatca tcctggattg gagatttacg gttattaatc agttcaacct tctctggaat
     3421 gatatccacc gctgtaacct tatggtgctg tgccaacaga gtggcaatac tcaaaccaac
     3481 ataaccggtg ccggcgactg caatgttata ctgtttcttc ataatatttc ctctttccac
     3541 cactgctgtg gtcactactt tcttaacaaa atgctgtttg tgaacagtaa cctgctattt
     3601 atataaaaca gcccgaaagc tgtgttcatt catacttaaa acagccagct cctacgccgg
     3661 caaattcaat ttttcataaa tcccctaccc gtttagggtt atattgcaag tttgagtcat
     3721 ttttattgac ctcgtttggg gttataatac attttcgcat tgaaatgtac ctacattttc
     3781 cactcaacca gtcaccaaaa agtttataaa ttcacttatt accctctatt cttactccaa
     3841 aggtatgttt aacatcgctt tattgaccta ttttcacccc aaatggaagt ttgtttggta
     3901 cattattggt ctattcccac cccaaactgt ctattttgag cgagtttttg ttttttatca
     3961 ttgcacattt tcaaggctca aattctaagc ggatttactg gcagggagac attggtaaat
     4021 acttgaacaa tttcacggct ttcatcatca catcctacg
//
LOCUS       DANKEF010000131         3900 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000131 DANKEF010000000
VERSION     DANKEF010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.135"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            45..380
                     /locus_tag="PWZ48_002869"
     CDS             45..380
                     /locus_tag="PWZ48_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262470.1"
                     /translation="MKQYLNEWKAIEGSLVTQRIKELPDCLEKDHLFQIREMLKKEQF
                     DPDQFLVVEYPTIGVYCCNHINDGKYFIIQEYGGQLTPFYTTWEMSEEGINNFPCESI
                     EDSISHTEC"
     gene            complement(637..2220)
                     /locus_tag="PWZ48_002870"
     CDS             complement(637..2220)
                     /locus_tag="PWZ48_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262471.1"
                     /translation="MFILDDEIKKVKNPRIIPLLEEVRSSFYQGNYRSAIAVNYSALI
                     LDLIDKLSDLANIYEDNTSKKILEDIARLRKSDPNSPKWEMELLEKAYSSTELIDSYE
                     YEDLKYIKDQRNYSAHPVVTYNGNTWELRDITKETCQDVIRKSYEIIFLKNPILGKKV
                     TIEIIQYASRIHAMSVTPEEFHSALKNSYLNKLSEKAKENLCKTAFKFVFQLSYGNEE
                     ADRDRESTFRLLNALIFDNKEFYLNILKKEIAKYRFTAESREEINKSLGENQQITYTK
                     GFFLLTFIATHPELQHDFSDETIQNLQISINDFKPKQQVLTEIEYYYRLRSLAVLGNN
                     NEELQNHLNSLVDPILQQKMRVSSLPSSTLETIYNQYLYFGEKEMFIDFMIEHLTDAS
                     SFRLADKDMEVLPWIYNKLNQEQFNKLLYNLNGNNQFYKNSNFPPFISNLLDFYNQKF
                     QVNLRKHYSGLLYPNAVTMDQGFQLKDKELKKLYELAESKSDMYLDIIDNIINKSEDY
                     LRKEINSEKELLNYVHKIK"
     gene            2342..2926
                     /locus_tag="PWZ48_002871"
     CDS             2342..2926
                     /locus_tag="PWZ48_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262472.1"
                     /translation="MDLKQSFYIWLYPDNPMSIMADIFVVSILLYLFYLIFFHNLIGY
                     MILDWKGMFDIERKPFANNQTYTVFESGDWEENEVDVIHEIGLALSGIQDADVLVSMT
                     TDTGYNAYEVFYLMYFIWRDGQEIINGRAEKTINSNTEISFCMGEGEMIVYKDGSANI
                     KTAFGDIETSIKELREYVQNPYNFQYLAGRINLV"
     gene            2937..3182
                     /locus_tag="PWZ48_002872"
     CDS             2937..3182
                     /locus_tag="PWZ48_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262473.1"
                     /translation="MKKIINQPTNHLIIEGVIFVTMYFTVGNGISNTIIQKNPQYLLF
                     SLYAWIVQMVWVSILMLLFYWIVRVILLLLNKRKKDR"
     gene            3188..>3900
                     /locus_tag="PWZ48_002873"
     CDS             3188..>3900
                     /locus_tag="PWZ48_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262474.1"
                     /translation="MKSTKLKVVLLSTAALITLTACGTTNSANNNKDTKSSSTMVTKN
                     STTSASTTMSSMTENNAEKYQKYEDINKGQKPDEVYNNFINTVKNDENYGNDPLQNKN
                     LTIVLYKPDCPDCQKAESKIMQEIKEGFSYAPTLMHDITINKPAYEYYAVAVDITDGV
                     PEWVQNIPGLADENNQYHTPTVAVVTPKKVNGTLYWDVMGMTTNTTDEMIESTTKYYM
                     DLGAYKLYQQSEPVMDDQS"
BASE COUNT         1375 a          527 c          624 g         1374 t
ORIGIN      
        1 caatttccta ggaaaaatag ttaacataaa aggaatgaat tgttatgaaa caatatttaa
       61 atgaatggaa agcgatagag gggtcattag taacgcaaag aataaaggaa ttacctgatt
      121 gcttagagaa ggatcatttg tttcaaatac gagaaatgtt gaaaaaagaa cagtttgatc
      181 cagaccaatt tttagtagtc gagtatccaa ctattggagt gtactgttgt aaccatatca
      241 atgatggaaa atattttatt attcaggagt atggaggaca gttaactcct ttctatacta
      301 catgggaaat gagtgaagaa ggaatcaata attttccatg tgaaagcatc gaagattcaa
      361 tttcacacac cgaatgttaa ttgattataa ctataaaaaa ggattgaaca ttatgttcaa
      421 tccttttttc aatgaaatgt tttccgccat ttcattgttt ggtgcgattt ctattttatt
      481 atatcaaaat aaagagagat tctaaagaaa gaagagggaa atatataaat caagcgacag
      541 tatatttatt attaaatgct attgtgagaa tataactaat tagtttaatt tgaaagtaaa
      601 tatacgctat aaaagtgcat cttgtgttat aaaaaattat tttattttgt gaacataatt
      661 taacaattct ttctcggaat tgatttcttt tcttaaatag tcttcagact tattaataat
      721 attatctatt atgtctagat acatatcact tttcgattca gctaattcat acagtttttt
      781 taattcttta tcctttaatt gaaaaccctg atccatagtc actgcatttg gatatagaag
      841 cccactataa tgtttacgta aattcacttg gaatttctga ttataaaagt ctaacagatt
      901 agaaataaaa ggcggaaaat tactgttttt gtaaaattga ttatttccat ttaggttgta
      961 tagtaatttg ttaaattgct cctgatttaa tttattgtaa atccatggta atacttccat
     1021 atccttatct gctaacctga atgaactagc atcggtaaga tgttcgatca taaaatctat
     1081 gaacatctct ttttcaccaa aatataaata ttgattatat attgtttcta atgttgaaga
     1141 tggtagcgaa gaaactctca ttttttgctg gagtatagga tcaactagac tatttaagtg
     1201 attttgtaat tcttcattgt tgtttcctag aactgcaagg gaacgtaaac ggtagtaata
     1261 ttctatttct gttagtactt gttgtttcgg tttaaaatca tttattgata tttgtaaatt
     1321 ttgaattgtc tcgtctgaaa aatcatgttg taattctgga tgggtagcta taaaagtaag
     1381 taaaaagaac ccttttgtat acgtgatctg ttgattttca ccaagtgatt tattaatttc
     1441 ctctcttgat tcagctgtga acctatactt tgcaatttct ttttttagaa tatttaaata
     1501 gaattcttta ttatcaaaaa ttagggcatt aagtagtctg aaagtacttt ccctatctct
     1561 atctgcttcc tcatttccat aagataattg aaaaacaaac ttaaaggctg tcttacatag
     1621 gttttctttg gctttttcgc ttagtttatt tagatagcta ttttttagtg cagagtgaaa
     1681 ttcttcaggt gtaacagaca tagcatgtat gcgacttgca tattgtataa tttcgatcgt
     1741 tacttttttt cctaatattg gattttttag gaatataatt tcataggact tccgtataac
     1801 atcctgacaa gtttcttttg ttatatctct taattcccaa gtattaccgt tgtaagtgac
     1861 tacaggatga gctgaataat ttctttgatc cttaatatat tttaaatcct catattcata
     1921 gctatcaatt agctcagttg aactataagc tttttctagt agttccattt cccattttgg
     1981 agagtttgga tcactttttc ttagtctagc tatatcttct aaaattttct tagaagtatt
     2041 atcctcgtaa atatttgcta aatcagataa tttatcaatt aaatctaaaa taagcgcact
     2101 gtaattgaca gctattgcag aacgataatt tccctgataa aaactacttc taacttcttc
     2161 taataatgga ataattctag gatttttaac tttttttatt tcatcatcta atataaacat
     2221 ataaatctcc tttttttatt tgtatgacaa gtttaccata aaaaaaaatt aaaaagcgag
     2281 acatatttat gttgttgtgt tatcataata acataaatat gtagagtgag atgatgaaaa
     2341 aatggatttg aaacaatcgt tttacatctg gttatatcct gataatccta tgtcaataat
     2401 ggctgatatt tttgtagtta gcatcttatt gtatttattt tatctaattt tctttcacaa
     2461 tttaatcggg tacatgattc ttgattggaa aggaatgttc gatatagaaa gaaaaccatt
     2521 tgctaacaat caaacctata ccgtcttcga aagtggagat tgggaagaaa atgaagttga
     2581 tgtgatccat gaaattggat tagctttatc aggaatacaa gatgctgatg ttttggtatc
     2641 aatgacaacg gatactggat acaacgctta tgaggtattt tatcttatgt attttatatg
     2701 gcgtgatggt caagaaatta tcaatggtcg tgctgaaaaa acaatcaatt ctaatactga
     2761 aatttctttt tgtatgggtg aaggggaaat gattgtatat aaagatgggt cagcaaacat
     2821 taaaacagcc tttggtgata ttgaaacaag tattaaggaa cttagggaat atgtgcagaa
     2881 cccatataat tttcaatatt tagcaggaag aataaattta gtttaggagt aaaaatatga
     2941 aaaaaattat aaatcaacca acaaatcact tgataattga aggtgttatt ttcgtaacaa
     3001 tgtattttac tgtaggaaat ggcatctcaa atacaatcat tcaaaaaaat ccacaatacc
     3061 tattatttag tctttatgct tggattgtac aaatggtttg ggtttccata ttgatgctgt
     3121 tgttttattg gatagtacgg gttattcttt tattgttaaa caaaagaaaa aaggataggt
     3181 gaataaaatg aaatcaacta aattgaaggt tgtattgtta tcgacagcag cattaataac
     3241 ccttacagca tgtggtacaa ctaatagtgc taataataac aaagacacaa agtctagtag
     3301 tacgatggtt actaaaaaca gcactacatc ggcatcaaca acaatgtctt caatgactga
     3361 aaataatgct gaaaagtatc aaaaatacga agacattaat aaaggtcaaa agccggatga
     3421 agtttataac aactttatta atacagtaaa aaatgatgaa aattatggga atgatccatt
     3481 gcaaaataaa aatctgacta tcgttcttta caaacctgat tgtccagatt gtcaaaaagc
     3541 agaatcaaaa atcatgcaag aaataaaaga aggatttagt tatgctccaa cattaatgca
     3601 tgatattacc ataaataaac cagcttacga gtattacgca gttgctgtag atattactga
     3661 tggtgtacct gagtgggtac aaaacattcc tggtctggct gatgagaaca atcaatatca
     3721 tacgccaaca gttgcagtag taacaccaaa gaaagttaac ggcacactat actgggatgt
     3781 aatgggaatg acaacaaata caactgatga aatgattgaa agtacaacta aatattatat
     3841 ggatttggga gcatataaat tatatcaaca atcagagcct gttatggacg atcaatccaa
//
LOCUS       DANKEF010000132         3857 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000132 DANKEF010000000
VERSION     DANKEF010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.136"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1812
                     /locus_tag="PWZ48_002874"
     CDS             <1..1812
                     /locus_tag="PWZ48_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262475.1"
                     /translation="TQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEI
                     KGRVDSLNQQITDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGDYDYSENPN
                     LMPYITEPWVGPLLGNGHTVKDSVKRVITHTKTRTANSGDILSLGLGIPCTAEANNRY
                     LITTLRPSTTYTLSVTMSVGSDWTGETNTIGVRLRYLNEQGGIELPINALIPANVERD
                     KMVTHTFTGITKDNVTSITNCYVEIFSLNSEYKGTVSVSYDVKLKAHYPNLLDGPYWL
                     GKVPLGENIADPTVVFPHKTSEYMVYGRRNTENYIADQTYTISMKATKLTVQSFAVYI
                     AAGRVKVGDMKPTEGLANTWELTFTVTKQHIDSGVTNYLEIYQYPSATKGAVQIEWLK
                     LEKGNTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVEDNLATDIKISEITGKANN
                     YTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDELDNKIDERINDTATTTLT
                     VTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSID
                     RSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            1829..1978
                     /locus_tag="PWZ48_002875"
     CDS             1829..1978
                     /locus_tag="PWZ48_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262476.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1975..2421
                     /locus_tag="PWZ48_002876"
     CDS             1975..2421
                     /locus_tag="PWZ48_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262477.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            2423..2560
                     /locus_tag="PWZ48_002877"
     CDS             2423..2560
                     /locus_tag="PWZ48_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2262478.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            2598..2891
                     /locus_tag="PWZ48_002878"
     CDS             2598..2891
                     /locus_tag="PWZ48_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262479.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKTLFRERRK"
     gene            2888..3112
                     /locus_tag="PWZ48_002879"
     CDS             2888..3112
                     /locus_tag="PWZ48_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2262480.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            3109..>3857
                     /locus_tag="PWZ48_002880"
     CDS             3109..>3857
                     /locus_tag="PWZ48_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HDL2262481.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWH"
BASE COUNT         1351 a          646 c          802 g         1058 t
ORIGIN      
        1 acgcaagata tcattttttt ctcactagga gtgtctgaca tttcgcaagg ccaagccgat
       61 gaatatgttt cgcaattaga agagttgatt cgaaagtaca acgaaacttt tgatgctttt
      121 atggctgaaa tcaaaggtag agtggatagc ttaaatcaac agattactga tttaactggt
      181 caagctaaaa cgctacaaga caagttagat gctctgaaag aagaaatttc taagttaggt
      241 aacttacaag tgatgtacag taacagcatc gacttcgggg actatgatta ttcggagaac
      301 ccgaatttaa tgccatatat tacagaacct tgggttggac ctttgttagg taatggacat
      361 acagttaaag atagtgttaa aagggttata actcatacta aaactagaac agctaattcc
      421 ggcgacatat tatcgcttgg cttaggtatt ccgtgtacag cggaagctaa taataggtat
      481 cttataacca ctttacgacc atctacgaca tacacattaa gcgttactat gagtgtcggt
      541 tcagattgga ctggagagac caatactata ggggttaggc ttagatattt aaatgaacaa
      601 ggtggtatag aactgcccat taatgcatta ataccagcaa atgtagaacg cgataaaatg
      661 gtcactcata catttactgg tataacaaaa gataatgtaa ctagcataac aaactgttat
      721 gttgaaatat tctcacttaa tagtgaatat aaaggaacgg ttagtgtcag ctatgacgtt
      781 aagttaaaag cacactaccc taacttacta gatggccctt actggttagg taaagttcct
      841 ttaggcgaga atattgctga ccctacagtt gtctttccac acaaaactag tgaatatatg
      901 gtttatggta gacgcaatac agaaaactat atcgctgacc aaacatatac catatcaatg
      961 aaagcaacta aactaacagt tcaatcgttt gccgtgtata ttgctgctgg ccgcgttaaa
     1021 gtaggagaca tgaagcctac tgaggggtta gctaatacat gggagttaac ttttactgta
     1081 actaagcaac atatagatag tggggtaact aattatctgg aaatatacca atacccgtca
     1141 gctacaaaag gagctgttca aattgagtgg ctcaaactag aaaaaggcaa tactcgaacc
     1201 ccgaatatta gtgagtataa atatcgtggt actggtatgc gtgattcaaa caatccaaaa
     1261 gattatgttt gggatctagc accagaatat gtcgaagata atttggccac agatattaaa
     1321 atttctgaaa ttactggtaa agcaaacaat tataccgatg ggaaagtatc ggagattaat
     1381 tcgcagttga ctgcttcaat taatgaagta gacaccacag ctaaggatgc tcaaacaaaa
     1441 gcgaatgcta atgcgactgc tatagatgaa ttagacaata agatcgatga acgcattaat
     1501 gatacagcta ctaccacatt aacagttaca aacgggaata ccggatcagc aaagctttat
     1561 cgtgaaggaa aaacagtttc tatatatttt gtggctttaa acggaaaaag cagtggtgga
     1621 aatgattcaa cgatactaac aattccagaa ggctatcggc caccaattag ttttgagcaa
     1681 ctggttggct cgatagaccg ttctactttg aacagtgctc agttatctat tggtgcagat
     1741 ggagccatta aatggcgaag aaactcaagt tatggatcgg attatacctt tgcaattact
     1801 tacacgattt agaaaagcgt gaatcgatat gaaggcagca tatagaccaa ttgaacctta
     1861 cggattcgag caaatcattg tgaatgatga agaacattta ccggaagaat gcacagaagt
     1921 cgaaccaccg attccaaatt ggaaaccgaa attcaattac tgggagggaa ataaatgaaa
     1981 aacatttgga aatatggacg tactggcgga gagtacgcag gaaaagtatt ggacgacatg
     2041 cttgtatccg ttccttacac agatcagcca ccgcttgaag gaattcgtgc tgatggcgaa
     2101 ccgttaacaa tcgctgatca aatgtttgat cctaaattga atcaatggat tattttagcg
     2161 aacgcactag atcacaacga tttaaacaat ctcaaagcaa tgtatgagtc gttagaaaat
     2221 gagaacggcg atttaaaaca gatcaatgcc aaactcatgc taagcgatgt agcaattaaa
     2281 caggaaaata ctgcattgaa agaaaaagcg gatagtttag cacaaatcaa ttcaaaaatg
     2341 atgcttgctt cgttacaaaa tagcaaagac atttcagaaa ttaaagagca actaaatcca
     2401 gcttcaaagg gaggtgagta gtatgtttag ttttagcgat gtgaaaatga tgtatgattg
     2461 gggctgtttc actaacgaac aagtaatggt tttcgttccg ttgtgcatta ctgaagaaaa
     2521 agcagataaa atcattagca aagaagagag cgcatcttaa ttgatgtgct ttttattttg
     2581 attcaaggag ttgtcacatg attaatttag gggaatgggg agcgatagca ggatcaataa
     2641 ccgctatcgt ttctttgatt ttattagtaa taaaaccaat tactgcatct ttctcgaaga
     2701 ttactgagac tctttcaaaa gtaagtcaca atttagattt gctgactaaa gatttagaat
     2761 cgagcaaatc agatcgattg atgattcatg aagaactaaa gaaacacgat gaaagattag
     2821 atacacatgc agaaaaattg gtagaacaca cgcaacaaat taaaacttta tttagggaga
     2881 gaagaaaatg aataataaaa cgttcgaagt actaaaatgg ttcgcactgg taattattcc
     2941 cgcactagct actttcgtgg ggttagttgg taaagcgctc aattggcagt acacagatat
     3001 ctgtgttgtc atcattactg gttttggcgc gtttttaggg agtgtgttgg gtgtatcaaa
     3061 tcgaacctac aaaatgttct cggctgaaag cgaagaagga ggaaacaaat gaaaaagaaa
     3121 attactatta ctgcgatgag cctgttaacg gctctttttt tattacccat taatacgttt
     3181 gcttatacta ttaatgacga gtataattta gcgccgaatc aaggagactc cagattagca
     3241 attcctaaca aaattatttt gcacgaaact ggaatagatg caccagcaag aaacgtagcc
     3301 gccaacatga aaaataatta taacggaagc aattcttata ctacagatgt tattggtgac
     3361 ggtgggattg tttaccgtgt gggtgagcaa ggatatgttt cgtggggagc tggtaacgct
     3421 aatccttatg cgcctgtaca gattgaatta cagcgcacat atgataaagc attgtttgaa
     3481 aaaaactatc gagcttatat tgaatataca agagatagtg caaaaaaata tggaattcca
     3541 ttgactcttg atcaaggaac ttctttattt acaaaaggaa tcatttctca tttgtgggtg
     3601 acaaattatg tttgggggaa ccacacagat ccatatggtt acttatcgca aatgggagtt
     3661 agcaaagaaa agcttgctta tgatttagct catggattta ccgatgaaaa tccaacaact
     3721 tctgaaaaca agcctgtcat tgatccaaca cgagctggtg cagctaatcc tacgctgaca
     3781 gatggaacaa atcacgccca cattgatcag tttggggaaa tcgaaaacgc aaacttgcat
     3841 gtggctggat ggcacat
//
LOCUS       DANKEF010000133         3856 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000133 DANKEF010000000
VERSION     DANKEF010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.137"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(50..301)
                     /locus_tag="PWZ48_002881"
     CDS             complement(50..301)
                     /locus_tag="PWZ48_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262482.1"
                     /translation="MEQLALVNLSALKASLAESEIANEVWDTKQAAEYLKTTTRTLTK
                     DAESGKIPAAKVGREWRFSSIALYQYLKGGKTWANSTEH"
     gene            complement(315..506)
                     /locus_tag="PWZ48_002882"
     CDS             complement(315..506)
                     /locus_tag="PWZ48_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262483.1"
                     /translation="MSNLDNGRAAIKKFMRENGVTMEDLATAYGYTRVRMQQIIDGHW
                     SGPKANKTILEIIRDYRIR"
     gene            799..1119
                     /locus_tag="PWZ48_002883"
     CDS             799..1119
                     /locus_tag="PWZ48_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2262484.1"
                     /translation="MTLFERIKSLASQRDKSMKEVALELGFSENLFYRWKTTEPKARD
                     LQKVADYFNVSVDYLLGREEKETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            1137..1559
                     /locus_tag="PWZ48_002884"
     CDS             1137..1559
                     /locus_tag="PWZ48_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HDL2262485.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFEAEADRIAIMILLNIFIENELTDE
                     SQFKLENFMEFYSINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            1642..2277
                     /locus_tag="PWZ48_002885"
     CDS             1642..2277
                     /locus_tag="PWZ48_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HDL2262486.1"
                     /translation="MAKKVMGQDGKMYKVKKPFYKRVWFWVLIVLLFFGIGGALSGGE
                     NKDSATATSTSTNKETTKETTTKEEKTYKIGDDVAVGKMEYKVNSVEVVKQVGPSVLP
                     TNAKDTFLVVDLSVKNAGDKAVTVDSSFFKLKADGKTFEADSAASMSANQDENGNITN
                     SFFMESLNPDMQQTGKIVFDISEAQANAQNNVLQAQTGYFGTETVSIALHN"
     gene            2395..3606
                     /locus_tag="PWZ48_002886"
     CDS             2395..3606
                     /locus_tag="PWZ48_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2262487.1"
                     /translation="MSEVTGYLEQVDNETYRLRAVLGYKPDGSAKRKSKTIKANSKRA
                     AYKELNIWLEQFEGMTDDSLDLFNITFGEFYRKIWLSEAEKNLEPKSYHNYKRMIENR
                     FLDKFDFIPLIDIKPYMIKKIVVNAQRINTKDPGRNSDKPLSRNTKLRMLYAVNNLFL
                     MAKNEYGAIKENPVENVKIPKEKGVKKNIEEPYSEEEIHAMLKAAFEESIEIKTLIVL
                     AFITGARQGEIAALEEKDIDFDKQEIRFHQRISEVDGKSDIRLLPGLKNDDDEKIVTG
                     PAYLFDMLDELIKENKKIRWKLNIKKLRHYFIFDTKQDGTLPRGSYLYKKFKRFTKRH
                     NLRHIRFHDIRHTSATYLLSDPNMTPKELQKRLGHRDFNTTMNVYGHVLRKEKDTATT
                     AFEKLLKKDIK"
BASE COUNT         1417 a          618 c          682 g         1139 t
ORIGIN      
        1 ctatttcccc aaagccctaa agcgtagatg attagcggtg cgctgaatac tagtgctctg
       61 ttgaatttgc ccatgttttt cctcctttta gatactgata caaagcgata ctactaaacc
      121 tccactctct gccaactttc gctgctggaa tctttccaga ttcggcatct ttagttagcg
      181 tgcgtgtcgt ggttttcaaa tattccgcag cttgctttgt atcccacact tcatttgcaa
      241 tctcagattc tgccaaagaa gctttgaggg ctgagaggtt aactaaagct agttgttcca
      301 ttgattaatt cctcctatcg aattctgtaa tcacggatga tttctaagat agttttatta
      361 gcttttggtc cactccaatg gccatcaata atttgctgca ttcgaacacg tgtgtatcca
      421 tatgcagtag ccaaatcttc catagttact ccgttttctc gcataaactt cttgattgca
      481 gcacggccgt tatctagatt tgacatctat tcactcccct tacatataga tttgtaagtc
      541 aaaatgatag aaaaaacgta taaactattg actaatagta tacaatcgta tactatataa
      601 acatagttaa ataagcctac aacaaaccct ttattatgca atcggtcgcc aaacttaatg
      661 ctataaggtg tatttttagt ttgctttttt tctatcaaat taacttacaa acaaataata
      721 atacaaactt atacttatgt caacagtata atttacattt tgtgtacttt tatttgttta
      781 ggattggaga acattattat gacactgttt gaaaggataa aatcattagc tagccaaaga
      841 gataaaagca tgaaagaagt cgctttagaa ttaggattta gcgaaaatct tttctatcga
      901 tggaaaacaa cagaacccaa agcaagagat ttgcaaaagg tagctgacta tttcaatgtc
      961 tctgtagact atcttctagg tagagaagaa aaagaaacgc ctaaacatgt ggatttatca
     1021 gaagatgata ctgtattttc ttttgatgga aaagaaatat ctaaggagac aatgcgtaaa
     1081 gcgattgcaa ttgctaaagc tttagaggaa aatgaatagt tggagtgatg ggttgtatgt
     1141 atttaaagtt gaaagaaatg ctgagtgagt ataatttaaa gttaatctat atggaaatgg
     1201 aagaaccagg tttttattat ccaaaaccaa gaatagtatt tttgaatgaa aaactacacg
     1261 aagacagttc tgaagctttt catttagccc atgagctcgg tcatttcatt gcttcacatt
     1321 ttgaatattc agtactgtac gataactcta caacttttca ttcaaaattc gaagctgaag
     1381 ctgatagaat cgcaattatg attttactaa atatctttat tgagaacgaa ttgactgatg
     1441 aatctcagtt caaattagaa aatttcatgg aattctactc tatcaataat aagttaagaa
     1501 cagaatgttt taatgtttgc cagtcatatt tcaagaaaaa atactcttat gcacagtaaa
     1561 aaaagcccgt gctgcaacac ggactcatac ctcatttctg agatcacaaa tatattataa
     1621 caagaagtga gggatattta aatggcaaaa aaagttatgg gtcaagatgg gaaaatgtat
     1681 aaggttaaaa aaccgtttta taaacgggta tggttttggg tattaatagt acttttgttt
     1741 tttggtattg gtggtgctct aagtggcggt gaaaacaaag attcagctac tgcaacatca
     1801 acaagtacta ataaagaaac tacaaaagaa actacaacaa aagaagaaaa gacttataaa
     1861 atcggtgatg acgttgctgt agggaaaatg gaatacaaag taaactccgt tgaagtagta
     1921 aaacaagttg gcccatctgt acttcctact aacgcaaaag acactttctt agtagttgac
     1981 ttatctgtaa aaaatgcagg tgataaggct gtgactgttg atagctcatt cttcaaatta
     2041 aaagcagatg gaaaaacttt cgaagctgat tcggctgcat ctatgtcggc aaatcaagat
     2101 gaaaatggta atatcactaa ctctttcttt atggaaagct taaatcctga tatgcaacaa
     2161 actggtaaaa tagtttttga tatctctgaa gcccaagcaa atgcacaaaa taatgtattg
     2221 caagcacaaa caggttactt tggaacagag acagtttcaa ttgctcttca taactaatta
     2281 gtataaaaaa cacgccccac cgaccaaagc gagcgtgttc taagaaaaaa caaacctaca
     2341 caataggctt ctttatagta cctattgtat cagagaaaga gagctgattc aattatgtca
     2401 gaagtaacag gatacttaga acaagtagac aatgaaacat atagactaag agctgtatta
     2461 gggtacaaac ctgatggctc ggcaaaaaga aaaagcaaaa ctataaaagc gaatagtaaa
     2521 agagcggcat ataaagaatt gaacatctgg ttagagcaat tcgaaggaat gaccgatgat
     2581 tcgttggatt tattcaatat aacttttggt gaattttata gaaaaatatg gctttcagag
     2641 gccgaaaaaa atttagagcc aaagtcttat cataactata agcgaatgat cgagaataga
     2701 tttcttgata aatttgattt tatccctcta atcgatataa aaccatacat gataaagaaa
     2761 attgttgtta atgctcaaag aatcaataca aaagatcctg gaagaaactc tgataaacct
     2821 ttatcaagaa atacaaagct tcgtatgtta tacgccgtta acaacttatt tttgatggct
     2881 aaaaatgagt acggagcaat taaagaaaat cctgtggaaa atgtaaaaat accaaaagaa
     2941 aaaggcgtaa aaaagaatat cgaagaacct tattcggaag aagaaattca tgcaatgcta
     3001 aaagcagcat ttgaggaaag catagaaatt aaaactttaa ttgttctagc atttattaca
     3061 ggagcaagac aaggagaaat tgcagcttta gaagaaaaag atattgactt tgacaaacaa
     3121 gaaataagat ttcatcagcg gatttcagaa gtggatggaa aatcagatat tcggttgctg
     3181 ccaggtttga aaaatgatga tgatgaaaaa atagtaaccg gtcctgctta tctatttgat
     3241 atgttagatg aattgatcaa agagaataaa aaaattcgtt ggaaattaaa tataaagaaa
     3301 ttaagacact attttatatt tgatacaaaa caagacggaa ctttaccacg tggaagttat
     3361 ctgtataaga agttcaaacg attcaccaaa cgtcacaatt tgcgtcacat tcgttttcac
     3421 gacattagac atacatcagc tacctatttg cttagcgatc ctaatatgac tccaaaagag
     3481 cttcaaaaac gattaggaca ccgagatttt aatactacaa tgaatgttta tggccatgtc
     3541 ttacgcaaag aaaaagatac agcaactaca gcgtttgaaa aactattaaa aaaagatata
     3601 aaataacact aaaaaattat attttcgggt ataaactcgg gtaggatata taaaaacttg
     3661 ggataatttg cggtatataa gcatttaaaa atgctgtaaa ttcgcttatc ttcatttaaa
     3721 ataccattaa ataacgaact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
     3781 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
     3841 cgctgttctt ttttta
//
LOCUS       DANKEF010000134         3785 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000134 DANKEF010000000
VERSION     DANKEF010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3785
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.138"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..171
                     /locus_tag="PWZ48_002887"
     CDS             <1..171
                     /locus_tag="PWZ48_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2262488.1"
                     /translation="SLFPLYHGSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFK
                     IEYTKKRQRLAY"
     gene            406..642
                     /locus_tag="PWZ48_002888"
     CDS             406..642
                     /locus_tag="PWZ48_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262489.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            649..2601
                     /locus_tag="PWZ48_002889"
     CDS             649..2601
                     /locus_tag="PWZ48_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2262490.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="PWZ48_002890"
     CDS             complement(2673..3170)
                     /gene="dfrG"
                     /locus_tag="PWZ48_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2262491.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            3400..>3785
                     /locus_tag="PWZ48_002891"
     CDS             3400..>3785
                     /locus_tag="PWZ48_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2262492.1"
                     /translation="MKYILSFCTPKLNYLLAYIRLYSGVLHLRDSVRVSEKEKIKVTE
                     MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRKKIENPHPLLQTTVE
                     PSKPEQREMLLDALLEISDSDPLLRY"
BASE COUNT         1344 a          614 c          655 g         1172 t
ORIGIN      
        1 tctctgttcc ctctttatca tggaagtgca aaaagtaata tagggattga taaccttata
       61 gaagttatta ctaataaatt ttattcatca acacatcgag gtccgtctga actttgcgga
      121 aatgttttca aaattgaata tacaaaaaaa agacaacgtc ttgcatatta agtagttcag
      181 ttttggagta caaaaagtga aaatgtaaac ctaaatgagt atcaaattgc aactgaattt
      241 gatactcgtt ttttattgtt aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag
      301 ttaaaatgta tataaaaatg cgaacgtgaa ataattattg taggaatgca aattggtgtg
      361 ttaaaatggt tacaatttac taagcaaaaa ggggtgcatt ttcctatgca atttcttgag
      421 ccaaaaaata agaacgctaa gtctgtggat tgggaaatat cagagcaagt aagggtgata
      481 gttaagcaat acgctgaata tgcagagcgt actgaaagcg aagcagtgga tgaattctta
      541 ttgaacattt tagacgataa gaagtttatt gaatggattg ccaacaagcg aagtaataag
      601 cggattgttg aaaaaatggg tatcaaagat agagtgggtt gatagacaat gaccaagctt
      661 aaaagattag ccacgaagga agatgaaatt gtagatgtta aaattccgat ttcaaacgaa
      721 gaattaaaag atagggctaa acaatatgat ttattaactc caaaacgatt tgcaacaagg
      781 tataacaaaa tgttgttctt gccaacgtct tttaaatgga acggttctga atatcccatt
      841 caatataatt actgcatcaa tccgttttgt tgtaattttg ggaaagaaca gcacaaattc
      901 aaagatgtaa aagggaaacc aagtcgatat aaaatgactg gtagttccaa agataaaggt
      961 cataaaggta tgtactgcaa tgacaatcct attggcagag gtgtatcgca aaactgtaca
     1021 gtaacccctt tatctaattg gtctgttgtt gaagaaatta aacgccttat agaaatcaat
     1081 agcatccaag atgttgagcc agattatcaa tttcataaag aaggctgctc ggaagaagaa
     1141 tcaaccccat tcaatgagcc gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct
     1201 caacgctatc aatgtaaggc gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa
     1261 accactactt atcaccaaca aaagaacacc attcttccaa tgttcgctaa gatggttgtt
     1321 ggaagggttt cagtcagtcg tacttgtgat attttaggaa ttggagttgg aacctactat
     1381 cataagctgg agtggctgta taggcgttgt ttggagtttt tagaacgata tgagacacaa
     1441 ccccttcaaa caaagaaatt taacgagatg tggcttaata cggacaagat gcactactac
     1501 ctaaataacg tccgtaagaa aggacaaggt tcaaagaaat atacggggtt tgaagacctt
     1561 aatatgcaaa cgtatatagt tgtatctgct gaggtcttat caagatatgt gttccgttct
     1621 gatgttgcct atgattggaa tatttcaatg gatgaattaa atgaagatac aagaaaattc
     1681 aaagaagacc atctcaatac ttttagtcgt aaaaatgata ggttagattg gtcttattat
     1741 ccacaagaac catccgctaa cgatagcgaa aataggaatg cttatcttca cgaattaggg
     1801 aaaattacta ataggagtag atttgtcgat ggtttaaatg ttgatgctcc ttatactaca
     1861 actgctcact attggttaat taaacaaatg gttaatgccg acgaatggcg aatgattagt
     1921 gatgatgact tctctattag aaacgccttt tatagagtat tcacgaaaga attaaggttg
     1981 agcgatgcac atcattttat ttgccaagtt aacaagacga aaagcagaaa acaatgctta
     2041 aaagaattcg gacaagcaaa agcagaactg ctcgattggg gagatatcag aggattcaaa
     2101 acaaagtttc tacggacttt ggcatctcat tatctaactg aattactaac aagtcatcaa
     2161 ttccacgaag aggctattag taaagatgga gaaagatatc gcaaatatgc cgataaccct
     2221 atcaaacatc cattagccac taaggataaa ggattttatt cagttgattg tagaacggat
     2281 ttatctgctc tggaaccaaa cgaaatcgca aagatgctat taaatgttaa tgaccattcg
     2341 accaatagct ttattcaaca gataagacgt tatatatcat ctcttgaaag accattaaca
     2401 acagctcgtg gtgataagaa gagctatatt tacgctaact tcaatccgaa gtatgctcaa
     2461 tttgcgatta caattctaag aacttactac aacttttgtc gcccttttaa atcagcggat
     2521 aaaaaggttt tgacacctgc tcaaagatta gggattacag ataaacaatt tgattggaaa
     2581 gatataattt atttcaaatg acaaaaagag cttggagtaa tcccaagctc ttgtgcttta
     2641 tctttactgt tcttcttcaa ttaacgcacc cgttaactca ataagttttt tctttcatat
     2701 acatgaaaat agtagttata cggattttta tcattcttta tccctttttg ggcaaatacc
     2761 tcattccatt cctcataatt cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt
     2821 atttttgtga tgtacatttt ctcaacataa gggaaaaaca aattataaat ctgttctcct
     2881 ccgaaaataa aaatttcttc ttcgttttta cataactcaa aaacatcttc tattgaatga
     2941 acaatttcac aaccattaaa ggtaaacccc ttatctctcg tcagaataat atttcttctg
     3001 tcaggtaagg ctcttccgat tgattcaagg ttcttcctac ctaatattat cggatgtccc
     3061 tttgtagtat ttttaacata ttcccagtcc ttgggaatcc tccaaggaat gtcattctct
     3121 ttgccaatca ctctattctt atccatcgca gcaatcaaag aaactttcat ttataacaca
     3181 gcctttttaa ctaatttacc atctaatttc ttatttctat caaaaaacct tttctcatta
     3241 tcctgcccgt tagttgaata agcaattttt tctataagtt atattatcat aataccacaa
     3301 aatcacaaat tttcattcaa ttatcatttt caattgcatt ttataaatca ttttgtatgt
     3361 aatttggtat gtaagttaaa atgaaaaaag tagtccattt tgaagtacat tttgtcattt
     3421 tgtactccaa aactgaacta cttacttgca tatatacgcc tttatagtgg agtactacat
     3481 ttacgagatt cggttagagt atcagaaaaa gaaaaaataa aagttacaga aatgtatact
     3541 tcaataaatg gtgaattatg taagattgat agagcttatt ctggagaaat tgttattttg
     3601 caaaatgagt ttttgaagtt aaatagtgtt cttggagata caaaactatt gccacagaga
     3661 aaaaagattg aaaatccgca ccctctacta caaacaactg ttgaaccgag taaacctgaa
     3721 cagagagaaa tgttgcttga tgcccttttg gaaatctcag atagtgatcc gcttctacga
     3781 tatta
//
LOCUS       DANKEF010000135         3730 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000135 DANKEF010000000
VERSION     DANKEF010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3730)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3730)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3730
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.139"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..481)
                     /locus_tag="PWZ48_002892"
     CDS             complement(<1..481)
                     /locus_tag="PWZ48_002892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2262493.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     "
     gene            complement(726..1997)
                     /locus_tag="PWZ48_002893"
     CDS             complement(726..1997)
                     /locus_tag="PWZ48_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDL2262494.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            complement(2281..3228)
                     /locus_tag="PWZ48_002894"
                     /pseudo
     CDS             complement(2281..3228)
                     /locus_tag="PWZ48_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634498.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            3240..3713
                     /locus_tag="PWZ48_002895"
     CDS             3240..3713
                     /locus_tag="PWZ48_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2262495.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
BASE COUNT         1206 a          694 c          596 g         1234 t
ORIGIN      
        1 ttgcttttgt taaatcagta taaactgcat ctacttcagc ttcaatacta gggtcatcaa
       61 tcactacgac attcgaatta attgctgata cttcttcaat cacagaagat agcacctgcc
      121 caataatcaa cacaccttga cattttagca aatcgtcttg ctccaacggt gtttctgaca
      181 aacgaataat tttcttcaaa ttgactttgc tttgttctgc ttcgacttga atccctctgc
      241 gaatcgttcg aaaataggga tcttccaact catctacttc agaaactgtc gttattaatg
      301 caatatttgt taaatttttt gcaacgggac tttttttgac ataatgataa aaattaaccg
      361 attctgcaat ctttcgtttg gtttcatcgg ttactgaaag tgtcggatcc tcatttagca
      421 cgcgcgatac tgttgcaatg gacacacccg cccgttttgc aacatcccta attcctacca
      481 taatagctcc tcttttcata tttgaaagtg tttactaaca tataatcata cattttgaaa
      541 ataagcaaat ttcgttaaaa ataggcactg taatcataat taaaaacaag aaaaaatatc
      601 cgaacaattt agagtaaata atgctaattc tagtgaattt gcgacgagga actgctagat
      661 cacagttcat tctctctatc gttcggatat tttattgtta actatactta tttcacagag
      721 cttttttatt tgattttgtg aacagagtct cgagaaatca attctccctc taatagtagt
      781 acttgatttt ttatttcttc ttgtttggcc agtccaatca gttggtgaaa agcaatcgcc
      841 cccttgttac ctggatttag tcgtattgtt gtcaacgcag gatccattaa ttctgcaaaa
      901 gtaatatcat caaatccaat aatcgataat tgttccatca ctcgtttatt cttatttttt
      961 aaaaatttta ctaatttagc cgctaaataa tctccagtaa caacaattgc agtaaattca
     1021 gctaattctt gataaattcg ctcaaattca gagttgttat atatccaatg cgatttaaag
     1081 gttaaatgat atttctttaa tgtatcgata taagcttgat gtcgttcttg aataacactt
     1141 ggttcaccta caatgtagtc aaaagaaaga aacgctattt tctcatgacc ataatgaatc
     1201 aaatgttcca ctgccaatgt actagcttgg taatcatttg tatttacaaa taaacgattc
     1261 atttgttgtt caagttctct taatgtctct tttgaaagat gcgtatcaat ataaacaaca
     1321 ggaccatcaa aaacgttatt taattccaaa aattctttta cacgtacacc aacaacaatg
     1381 aacccctcaa aacgccaatt tctctgaata gttttaacat cttctgctga attaacatta
     1441 tgcaccaata caaaataacc atgattcttt gcctgttcct caattccaga caaaacttct
     1501 gcggtaaatg gatcagaaaa tacaaaatca gcattatcaa tagccgaaaa ataaagcaat
     1561 cctattaaaa atgcaatcgt cttaccgatt ccctgacaga cagaacacat tccactagga
     1621 tcattaaatg aataagcatt tgcatatcct aaactaggtg ttgcaaaaca tgaaaacaac
     1681 acacgaaata acgcattgat gttagtaatc gtccctaacg ttgagcgcgc actgccacca
     1741 atcttctttt gattgaccac aatcgccgta gaaagattat caatctcatc aacctctgga
     1801 cgactgtatt taggtaaaaa taaacgggta aaactatcat aggtgtcatt cagttgacga
     1861 ccagcttctt gggtaatagt atcaaaaaca atcgatgatt ttcctgaacc agatacgctg
     1921 gtaaaaatac taattttatt tttgggaatt ttgagactga catttttaag ttgtttctct
     1981 tgcgtggata atttcaatat actcgttcat ttggtactcc cctttatttt gaaatagttg
     2041 agaatgtttc aatgagacac ggtatttttg gcagatgttt agtttaccta gaaagaggta
     2101 taaaaatcaa gaaaaaaaga gaggctgact cataagtagt tttattagtc gaaaatgata
     2161 gtttataaaa ataggaaccg catcaaatca acattcttta gaatggattt gatgcgtttt
     2221 tctttatttt ttgacttatg agtcaacctc ttactcttcg tcatggattt gattagctaa
     2281 ttattgctcc acttgttcac gaatgaaatt taagtactgc ggtaaaaata aaggaatttc
     2341 ttcaaagcgt ggactttgat tttcatacgc ttttgcatct aggtttcctt cccctgtagc
     2401 aatttcaatc aataattgat ttgggtcacg ataataaagt gatacaaaaa attcacgatc
     2461 ttttattcct gaatttataa aattgcgctc ttgtaattgt ttgtcgatgc gttgtaaatc
     2521 agatttattt tctacgccaa acgcaacatg atggacgcta ccaatccctt caatggcaac
     2581 tggattctca cgatcttgaa taatctgaac ttcttgatag aactgagtgt cttgattttc
     2641 taagattgtc actcgttgat gacccgaaaa gaaactgacg tattctttgt gctaccagcc
     2701 taccgacaat aactctcgtt cagtcgcatc ggaatattgt acgcgtagtt gaatggcatc
     2761 aattcctaaa atggcatgct ctgtagggat ctggtctgtg acatacggga aataatcatc
     2821 aatatcttca aattcgcgta agggaactaa agctaatcgt gttgcatctg gcccttcaaa
     2881 acgtaagata ggtcgtccag aaaatgattc gattccataa tggcaaacgc cttgtgtttc
     2941 aaaatatatt tcccaaaaat gcaatgatgc ttcggaagga acttttagta tcgttcgttc
     3001 aatcgtattg gttccaaaaa actggttgtt cccttcttgt acttcaaaaa aagtcagttc
     3061 agttcccaca cggtggtgat tatctgaaaa aaacaggtga tacatggtat gatcgtcttg
     3121 attaattgtt ttcattagta attttaaacc gagtatatta tgataaaagt caaatgtcgg
     3181 tcgaataaag cgattcacaa tagaaatgtg atgaatacgt gcgggcataa tatctaaaaa
     3241 tgagaatcaa acctataaaa gatggtgaac aacttttgtt ttcatcagaa gaaaataagc
     3301 ccctttattt agactatgta aatcacaatt tgaaaatcat cattaaagag aataacttaa
     3361 aacatatcac acttcatggt tttcgtcata cccattgtag tctgctcttt gaatctggtg
     3421 cgtctgtcaa agaagtgtat gttcgacttg gacatacaga tattaaaacc actatggatg
     3481 atatatacac ccatatcgcg aaaaagcaga cagaggaaac ggcaattcgt tttgcagatt
     3541 tcatgagttc tacagaggaa aagcaaggaa gtttgcagaa ggtcaaaagt atggtcaaaa
     3601 acgaaaaaag cagactatcc ctattagata gtctgctaaa cgttgagtta tcagtatttt
     3661 tcttattccg cagcgtttgc gtttttgaga ccgtattgat tctctttttt taatttcatc
     3721 cttttccaac
//
LOCUS       DANKEF010000136         3610 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000136 DANKEF010000000
VERSION     DANKEF010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3610
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.140"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..844
                     /locus_tag="PWZ48_002896"
     CDS             <1..844
                     /locus_tag="PWZ48_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2262496.1"
                     /translation="EASKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKA
                     GEVAQARGAVTVGLTHNKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNV
                     TETFEHYGKFREGMKVIDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAY
                     GFSICSLMEMQWLDSTSVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLD
                     KYAERVEIVDAKELGLDMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMG
                     KVEY"
     gene            855..1670
                     /locus_tag="PWZ48_002897"
     CDS             855..1670
                     /locus_tag="PWZ48_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HDL2262497.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            1738..2448
                     /locus_tag="PWZ48_002898"
     CDS             1738..2448
                     /locus_tag="PWZ48_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDL2262498.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            2484..>3610
                     /locus_tag="PWZ48_002899"
     CDS             2484..>3610
                     /locus_tag="PWZ48_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDL2262499.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1199 a          541 c          806 g         1064 t
ORIGIN      
        1 cgaagcatcg aagtgtatta caggcttata tacagcgaac gaatttaacc atgtaacccc
       61 aaagcaatta gatgataaat ctgttgtcat tctatgttct catggaggaa atacagcaga
      121 aacggtaaaa gcaggagaag tcgctcaagc tagaggagct gtgactgttg gtctgactca
      181 taataaaact gcggatttat taaaggtttc tgatcatagt ttcctttacg aatggggaga
      241 tgatgcaaaa atagtcaata atcctatggc catcattctt gatttaactg tatctttggt
      301 caatgttacc gaaacatttg aacattacgg taaattcaga gaaggcatga aggttatcga
      361 taccgtaatt gaaaatggca ttaagcaggt gcaggatcgt attaaagtgt ttgctgataa
      421 gtacaaagat gaaacaatgt tttatatctt aggtagtgga ccatcatatg gacatgctta
      481 tggtttttca atttgctctt tgatggaaat gcaatggctt gattcaactt ctgttcattc
      541 tggagaattc ttccatggac cttttgaagt aacagacaaa gatacgaatt ttattctctt
      601 gatgagcatt ggacgcactc gtgctttaga tgaacgggca aaacgattcc ttgataaata
      661 tgcagaaaga gtagaaatcg ttgatgcaaa ggaactagga ttggatatga tatcagatga
      721 agtttctgaa ttcttcaatc ccattttatt ctacagtatt ctttcagaat atcgttcagc
      781 tttagcagat gtacgcaatc atgatttaga tgttcgtcgc tatatgggga aagtggagta
      841 ctaggaggat taaaatgaag gtattaggct taggtgataa cgtcgtcgat aaatatgaaa
      901 atctgggaat tatgtatcct ggaggaaatt cattgaattt tgcagttttt gcaaaaaaac
      961 tgggacatga aagtagcttt atgggagttt ttggctcaga tgaggaggca gctcatgttt
     1021 tatcagcaat aaaagaaata ggactggaca acagtcactc tcgctttgaa aaaggcgaga
     1081 atggctgtgc cagagtgaag attgatgagg gcgaccggat tttccttggc agcaattcgg
     1141 gaggtgttac tcgtgaatac ccgattcagc ttactgaaga agacagagaa tatttaaacc
     1201 agtttgaatt gattcatatg ggattatata gccatgtcaa tcatctatta gaagagttac
     1261 aaaatgtttc tggaaagatt tcttatgatt tttctgacga ctttacggaa gaagaggttc
     1321 agcgggcaat tgataaagtt gattttggct tcttttctat agaagacttt tccgatgaag
     1381 aagtaaagca gtttttgatg aagcattttt gtgagcgaaa tgatgtattg attgcaaccc
     1441 gaggagaaaa aacggctatt ggttatgatg gagcaaagtt ttatgaagtg aaccccgtgc
     1501 tagaagaacc agttgataca atggcagcgg gagattcttt cttaacagct ttcctacttt
     1561 attttctcga aggggacgat atagtcaccg cgatgaaaaa ggggaatgaa tttgctggga
     1621 agtcttgtct ggttgacggt tcgttcggct ttggtattca ttacgattga gcttgtttta
     1681 aattcctgca tttgataacc taaagaaaga aatcccggta aagtggaggt taaaaaaatg
     1741 ctggatacaa atgcacaatt accattgtac ttacaattaa aagaaatatt aaaaaataaa
     1801 atcaatacag gggagtatcc cgagaatacg aaaattcccg ctgaatcaga gctgatagaa
     1861 agttatgagg tcagccgaat cacagtgaga aaagcagtag aagaactggt agaagaaggg
     1921 tacctactta aaaaacaagg gaaaggaact tttgtcaacc ggcataaagt ttttcgtaaa
     1981 attgaatata ttatgggttt cagtgagagt tgtaaatcta atggttttac accgactagc
     2041 attgttttgg aacgcaagat cattccagca actgaaggga tggcaaaaaa actcgatatc
     2101 gctcaaggtg atgatgtttt atatatccag agaaagcgaa tggcggataa catccccatt
     2161 atgttagaaa acaattattt ttcttatgaa agatatagtt tcttaatgga ggaagagctg
     2221 actgattctt tgtatgaagt acttaaagtc caaggtacct tagccatcaa tcctggggaa
     2281 actgcacttg aaattgtcac agctgatgac caaattgcca aaataatgga agtagctgtt
     2341 gggacgccct ttttctttat ggatacttta atcaacgatc aaaacaacat accaattcat
     2401 gtaggtaatc aatattacct gggagaagta tatcgttttt acttatgatt tcctgtttgg
     2461 atttcaagga gtagatttta tttatgaagc aaatattgcg gtcaaaaatg atttttgatg
     2521 gagagcagcc acctttcaaa ggttatattg taatcaatga agggatcatt gaaaaggtgg
     2581 aaaacaactg gaattacagt gatctattaa atgaaaacac ggaattaatt gcttatgatg
     2641 atcaattcgt tatgccagca attcatgata atcatgtatt tttcagtggt tatttggcta
     2701 tgaatgctgg catagattta agcgatacat cttcagctaa cgaggctgta gagcgtatta
     2761 aagctgtaat gaaccataaa aaagcagatg aaccaattta tgctcatggc tgggatgctg
     2821 ccctgtggaa aactgaccca tcagaagagc tattgaatga aataaaccac tttggtccaa
     2881 tcacagcgat cgacaaaaat cgcagccatt gttggatgaa tgctattgct aagcagactt
     2941 atggttttac agaaaaagaa accagtgctg aaagtcgagt attattaatt aaggaaatgc
     3001 tagcaaacaa aaagctttta gcagaggttt acgctgaatt tgaagcatta ctgctttcaa
     3061 aaggtgttat atcaatgaaa gaaatcgttt ttgatgacgc agaatttctt tctaatatac
     3121 ctcagaggaa aatacttagt aacctatatg tgcaggcagt ccaagaacct ctacagcatg
     3181 aacttctata tgagtataag gaaaaagttt tccctgaaaa ggtccgcttt ggtggagtga
     3241 agatcatggt agatggtgtt gttgcggata gaagtggaga tatttacggt gtttatcagt
     3301 caggagtagc ttcacccgag attgactatg aggcaattaa agcagaagtt gctgcattca
     3361 atgaagtaga gattccttgc tgtttgacaa cggaaggaga taaagcgggt tataaagctg
     3421 cgtcaattct tgtcgaattt ggaaagcggt tacctgaagg cgtttttaat tcgattagtg
     3481 atttggaaat ggttactcaa aagacagcag aaaaaatgaa cgaaggacaa gtagtagcag
     3541 aggtatatcc acaaattctc ggcttgaatc aaacgttaga agaggcttac atgtcaactg
     3601 ttataaaaaa
//
LOCUS       DANKEF010000137         3460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000137 DANKEF010000000
VERSION     DANKEF010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.141"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1306
                     /locus_tag="PWZ48_002900"
     CDS             <1..1306
                     /locus_tag="PWZ48_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDL2262500.1"
                     /translation="LANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVLETPYWYAKK
                     ILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTASSMANVANR
                     MLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQLGGLLVNHL
                     NGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPKKKKKKAIIT
                     CCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQLYDVIMIVGT
                     EDPKINQIPYIGLDRLINGGSIGEFVHYLEKELTIEVNDLQKELIFNFSISKIVENLT
                     ILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKESVDEQEDIE
                     EYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            1319..1951
                     /locus_tag="PWZ48_002901"
     CDS             1319..1951
                     /locus_tag="PWZ48_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HDL2262501.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2156..2383
                     /locus_tag="PWZ48_002902"
     CDS             2156..2383
                     /locus_tag="PWZ48_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDL2262502.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2452..2718
                     /locus_tag="PWZ48_002903"
     CDS             2452..2718
                     /locus_tag="PWZ48_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2262503.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2708..2977
                     /locus_tag="PWZ48_002904"
     CDS             2708..2977
                     /locus_tag="PWZ48_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2262504.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
     gene            complement(3156..>3460)
                     /locus_tag="PWZ48_002905"
     CDS             complement(3156..>3460)
                     /locus_tag="PWZ48_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262505.1"
                     /translation="YYQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYES
                     GIRLALELPYSNAKIENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT         1204 a          505 c          695 g         1056 t
ORIGIN      
        1 tttggccaac tatctatatg tcaaggataa tcaagtgatt tttgaaaata gtcttcaaga
       61 aaagaaaacc aaagatcagc ttaacgaatt ttttgctaat gttcttgaaa ccccttattg
      121 gtatgcaaaa aaaatcttaa gccttttatc tgagtgttta gatcaacctg tgtatgaaga
      181 ggacattttc tttattacgc tatattttta tagtttgaat gtcacgaatg ttaaaacagg
      241 tattaagagt attgtgctag ctcatggtta ttctacagct agtagtatgg cgaatgttgc
      301 caaccgtatg ctgaataaga acgtttttca agcttatgat atgcctttag atattacatt
      361 agagaaaatt gaagcaaaat tgattcaatt ccttggagat tatcgaacag attcaggctt
      421 gattttattg gtagatatgg gttcactaaa ccaactaggc ggtttattag tgaatcatct
      481 aaatggacca ctgttaatca ttgatcatat caatacaccg ttattattgg aagtcggcca
      541 acaagtatta aacaataaaa gtatcaccga aataggagca gaaattgaaa caagtcttac
      601 tttcaaaaag cagttactac ttcccaagaa aaagaaaaag aaagccatca ttacttgttg
      661 ttatacaggg attggtagtg cgacccagat tcaggagatt ttaacgaaat gcttgaaaga
      721 tgcagttaga gaattagtaa ttattccgta tgattacaaa aagctgttag aaaacaagaa
      781 ttttgagact ccttttcaat tatacgatgt catcatgatt gtaggtacag aagatcccaa
      841 aattaatcag attccatata ttggattaga tcgcctgatt aatgggggaa gcattggcga
      901 atttgttcat tacttggaaa aagagctgac tattgaagtg aacgatttac aaaaagaatt
      961 aattttcaat ttttcaatta gtaaaattgt cgaaaattta accattttgg atccagataa
     1021 ggtgctatct tttgtacaaa aagcaattaa agaattatcg aagttattaa attttgattt
     1081 ttctaataat cagctgtttt tgttgtacct tcactgtagt tgtatgattg aacgaattgt
     1141 acgtaaagaa agtgtggatg agcaagagga cattgaagaa tatatgcaag caaatggaga
     1201 aataatggat aaaattcata gcgctttttt tgagattgaa agggaataca caattgaaat
     1261 accagattta gagttgcgat tattgagcga tattgttcaa ggataggagt gaattcgaat
     1321 gacaatggga gtaatttttg atatggatgg ggtactcata gatagcgaga cattttattt
     1381 tgagagaaga atgaacttct ttcaagaact ggagatagat ccaggatcaa accagattga
     1441 tgattatgtg gggaaaacag aagctgcaat atgggaaaca ttagttcctg aggacaaatc
     1501 attaagagag gaactttatc cgaaatatgt agattatcga aaaacgcatc caattgattt
     1561 cgctaaagct ttacgaaaag aagtgaaacc gttacttgac ttcctcaaga agcgggatat
     1621 accattggca ctggcatctt cttctccagc tgtcgagata gagagtatgc tcattcaatg
     1681 tgaacttact gaatattttt cctttgttat aagtggtgag tcattaaaac aaagcaaacc
     1741 acatcccgaa atatatttaa aagcgaaaca gcaattagac tgtgacttct ttattgcagt
     1801 agaggattca acgattggta ttaaatcagc aaaggcagca aatatctata cggcggcact
     1861 aaaacaacca atgcaaatcg atcagagtga agcagacata caaattgaaa gtctacaaga
     1921 actggaacaa attgttttag aaaaaggtta gtgaattaat ggaaataagg ctatatatct
     1981 gtgctagcta ttttgactaa ccaaaaaaac ttgctataat tcaggtgtag taaaaggtgc
     2041 tggtaacacc ttttacgaaa atgcttcggc agtgataatg ggattggctt tttaagtttt
     2101 ttattttgtt cgcaatccct aaggtcatgt gaatgatcct agggattgct agaatatttt
     2161 gcaacagaac caaaatacga atagttatca agcagccata ttgactgcgg tcaatttagg
     2221 aggggatact gatactactg ctgccgtaac tggtgctctt gcgggtattc tttacggggg
     2281 tgaagtgatc cctgaaaatt ggagaaaatc gcttcaaaat gaccagcttc ttaaaacgat
     2341 cagtcagaga ttttgcgata ccgtgctatc tcaaagaatt tgaataatgg atatgatgca
     2401 aatcttgttt gtactaacgt tgtattgaca aaaaggattt tatttagtac aatgttagta
     2461 caaatgatga acggaggtag aatcatgtct caatcaacca ttacatttag gattcccgac
     2521 gatgaaaaag aactggtatt agaatatgca aaagcacaca attcttcatt gaccgaactt
     2581 tatcgaaatg ctgtactgga taaaatcgaa gatgaaatcg atctacggac tttacggagt
     2641 gcaattgaag tttccaaaga gaaaaaagag agtggtattt ctcaagagga aatggagaaa
     2701 ctgctaaatg aattataaag tcatctacat gccgcaagcc cagaagcaat taaaaaaaat
     2761 ggacagaaac caagctaaaa taattgtcgc ctggatcaga aagaatttag aaaacacgac
     2821 tgacccgcgt cagcacggaa aaggtttgac tggaaataag agtggggaat ggcgctatcg
     2881 aattggaaac tatcgaatat tggctaatat ttgtgattct gaaattgaaa tccagatatt
     2941 ctcaattgga catagaagta caatatataa aagataaccg tttttggaac gaagtagagt
     3001 ttcgaaagtc aatgtataat cttttgaatt ttgcataata ccacaggtag aaactacccg
     3061 atactatcgg ttggtttctg cttttttatg gctctttgtc aaataggact gatagagtga
     3121 gataagaaca tctaaggtag aattcctttt ctactttaga tgttttttga ttcataggta
     3181 atcagattat ttagtaagaa aattctggtc ttcattttcc gaaagcttcg aaagccataa
     3241 gcgactcgtt tcaatgcttt gatgtgggta tgcagatttt ctattttggc atttgaataa
     3301 ggcaattcga gggctaaacg aatgccggat tcatagttta taaaagcttt ttttatagaa
     3361 tgcagttctt ttggaacgga attgggcatc gttcttacaa gtttaaagaa agtgtccgcc
     3421 tctttgtcgt gaaacgcttg aagaatttct tgatagtaaa
//
LOCUS       DANKEF010000138         3330 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000138 DANKEF010000000
VERSION     DANKEF010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.142"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..734)
                     /locus_tag="PWZ48_002906"
     CDS             complement(<1..734)
                     /locus_tag="PWZ48_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2262506.1"
                     /translation="MKKKIFVGAIIALFLLPINAFAYTINNEFNLGANEGSSQVANNQ
                     YILLHETANETATGRNEAQYMKRSWYNAYTAYIVGDGGIVYQVGQPGYVQYGAGSYAN
                     ANSPVQIELQHTHDKVTFEKNYKAYVELARDSAMKYGIPLTLETPYNQPGIKSHLWVT
                     QNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAANPTL
                     TDGTNYAHIDQFGEIENANLHVAGWH"
     gene            complement(731..1150)
                     /locus_tag="PWZ48_002907"
     CDS             complement(731..1150)
                     /locus_tag="PWZ48_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDL2262507.1"
                     /translation="MEKYLNTFSVMTGVIGGTIVGLLGGMDNILHVLIFLVGVDFLTG
                     LAKAWKLKEISSEIGFEGLLKKVLIFVVIAVAVEVQKIVGNSIPLREIVIMFYVANEG
                     ISFLENISVFLPLPDKLKEVFQQIRNDTENKDRGGTK"
     gene            complement(1220..1360)
                     /locus_tag="PWZ48_002908"
     CDS             complement(1220..1360)
                     /locus_tag="PWZ48_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HDL2262508.1"
                     /translation="MNSFPGFENIKQFYDWGCYTDQDLLDYVNMNCLTKDQYKQITGN
                     EI"
     gene            complement(1353..1700)
                     /locus_tag="PWZ48_002909"
     CDS             complement(1353..1700)
                     /locus_tag="PWZ48_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262509.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKSGTAVDDIEDVDQLKQLNALLTKQLAISVQEQEKMQQMLAQLTM
                     EVASIKNGGKSNE"
     gene            complement(1714..>3330)
                     /locus_tag="PWZ48_002910"
     CDS             complement(1714..>3330)
                     /locus_tag="PWZ48_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262510.1"
                     /translation="TQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFLAEI
                     KGRMDSLNKQITDLTGQAKTLQDKLDALKEEISKLGNLHVMYSNSIDFGGYDYSGNPN
                     LMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTRINTPQLASGKTYTLSAKV
                     RFDEGTTGAIDKLRLVYRTSPGEKILLEANNTTITIDDVGKEITIKGTANVNYQITNL
                     DRFYISVSFSNRDKINGGFKLYDIKIEEGSTATPYQPNLLDDPYWVGKTSLGKNIANK
                     GTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQTFVAYNYWNVNFGDLKPV
                     EGLTDVWSLTFTPTKLEPGLPKDLRIFQSPKETAGACRIDWLKIEKGDTRTPNIEQYK
                     YRGIGMRDSNNPKDYVWDLEPSYVENNLATDIKVAEITGKANNYTDTTTLTVTNGNTG
                     SAKLYREGKTVSIYFVALNGKSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSA
                     QLSIGADGAIKWRRNSSYGSAYSFVITYSI"
BASE COUNT          898 a          686 c          551 g         1195 t
ORIGIN      
        1 atgtgccatc cagcgacgtg taagttcgca ttttcgattt ctccaaactg atcaatgtgg
       61 gcgtaatttg ttccatctgt cagtgtagga tttgctgcac ctgctctagt tggatcaatg
      121 actggtttat catctgaagt tgtcggattt tcatcggtaa atccatgagc taaatcatat
      181 gctaattttt ctttacttac gcccatttca gaaagataac cgtaaggatc tgtatgatcg
      241 ccccagatat tttgtgttac ccataaatgc gatttgattc ctggttggtt ataaggcgtt
      301 tctaatgtta atggaatacc atatttcatt gctgaatctc tagccaattc aacgtatgcc
      361 ttgtagttct tttcaaacgt tactttatcg tgcgtatgtt gcagttcgat ttgcacagga
      421 ctatttgcat tagcatatga accagcgcca tattgtacat aaccaggttg tccaacttgg
      481 taaacgatcc caccatcgcc aacaatatac gctgtatagg cattgtacca agaacgtttc
      541 atgtactgcg cttcatttcg tcctgtcgca gtttcgtttg ctgtttcatg caataggatg
      601 tattggttat ttgctacttg agaactacct tcgtttgcgc ccaaattgaa ttcattgttg
      661 atcgtgtaag caaaggcgtt tattggcaat aaaaaaagag ctataatagc tcctacaaag
      721 attttctttt tcatttggtt cctcctcggt ctttattttc agtatcattt cgtatttgtt
      781 ggaatacctc ttttaactta tctggcaaag gaaggaatac cgaaatgttt tctaaaaaac
      841 taatcccttc atttgcaaca tagaacataa taactatttc tcttagaggt atagaattcc
      901 caacgatttt ctgtacttca accgcaacgg caatcacaac gaagattaag actttcttca
      961 acaatccttc gaaacctatc tcactactta tttctttcaa tttccatgcc ttagcaagtc
     1021 cagttaagaa gtccacaccc acaagaaaaa ttaaaacatg tagtatattg tccatccctc
     1081 ccaataaacc aacaattgtc ccgcctatca cccctgtcat tactgagaat gtatttaaat
     1141 atttttccaa tatctatttc tcctttatgc aaaaaagcgc actcgtttga gtacgctttt
     1201 tatttaactt tgtgcttaat taaatttcat ttccagtaat ctgcttatat tggtcttttg
     1261 ttaaacagtt catgtttaca tagtcaagta aatcttggtc cgtataacat ccccaatcat
     1321 aaaattgttt tatattttca aagcccggaa aactattcat tacttttccc tccattctta
     1381 atacttgcga cttccatcgt caattgagct aacatttgtt gcattttttc ttgttcctgt
     1441 accgatatcg ccaattgttt tgtcagtaga gcatttaatt gcttcaactg atcgacatct
     1501 tcaatatcgt caaccgctgt gccactcttc tcttcttcag tagctaactc aacccactgt
     1561 tttttatcaa aatcaaatct tggtctccag ttaggaactg gtggtttaat ctctgtacag
     1621 ttttcaggga tattttcttg attgttcaaa atgatttgct cgaatccgta aggcttgatt
     1681 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1741 ataagctgat ccataacttg aatttcttct ccatttaatg gctccgtctg cgccaataga
     1801 tagttgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1861 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1921 tccgtttaaa gccacaaaat atatagaaac tgtttttcct tcacgataaa gctttgctga
     1981 tccggtattc ccgtttgtaa ctgttaatgt ggtagtatcg gtataattgt ttgctttacc
     2041 tgtgatttca gcaaccttga tatctgtagc taggttattc tcaacatagc taggttctag
     2101 atcccataca taatcttttg gattgtttga gtctcgcatg ccgattcctc ggtatttata
     2161 ttgctcaata ttcggggttc gtgtgtcgcc tttttcgatc ttgagccagt caattcggca
     2221 tgcgccggcc gtttctttag gcgactgaaa aatgcgaagg tctttaggca aacccggttc
     2281 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     2341 atctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     2401 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     2461 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctt
     2521 acccaacgat gttttaccta cccagtaagg gtcatctagt aagtttggct gatacggagt
     2581 ggctgttgag ccttcttcga ttttgatgtc gtacaattta aatccaccat ttattttatc
     2641 cctgttagaa aaactaacac taatataaaa acgatctaaa tttgtgattt gataattaac
     2701 gttagctgtg cctttgattg ttatttcttt ccctacatca tctattgtaa tagttgtatt
     2761 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2821 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2881 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2941 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     3001 gctcgatttt agtttggaca ttaaattcgg attcccacta taatcatagc ccccgaagtc
     3061 gatgctgttg gagtacatca catgtaagtt acctaattta gaaatttctt ctttcagagc
     3121 atctaacttg tcttgtagtg ttttagcttg accagttaaa tcagtaatct gtttatttaa
     3181 gctatccatt ctacctttaa tttcagccag aaaagcatca aaagtttcgt tatactttcg
     3241 aatcaattct tctaattgcg aaacatattc atcggcttgg ccttgcgaaa tgtcagacac
     3301 tcctagtgag aaaaaaatga tatcttgcgt
//
LOCUS       DANKEF010000139         3217 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000139 DANKEF010000000
VERSION     DANKEF010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3217
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.144"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            49..1437
                     /locus_tag="PWZ48_002911"
     CDS             49..1437
                     /locus_tag="PWZ48_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HDL2262511.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1775..>3217
                     /locus_tag="PWZ48_002912"
     CDS             1775..>3217
                     /locus_tag="PWZ48_002912"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HDL2262512.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMNMILHGVPI
                     ANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVLAPKS
                     KADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGLPANI
                     FFNTSIPTTVIILKKDRAKKDVLFIDASQDFEKIKTQNTLRDYHIDAILEAYKTRTIK
                     DKYAYVAEFDEIVENDYNLNIPRYVDTFEEE"
BASE COUNT         1183 a          505 c          582 g          947 t
ORIGIN      
        1 tcaaaaaaaa accaaccgaa agatataaaa gatattgtag atacacgaat gtataaagaa
       61 cgtgatgact taaactatac gcttattatc aataaagatg gaaactattc atggcgtcct
      121 ctcgttctac tacatcctat tctatatgta gatttggtta attgtctcac taaagaagac
      181 cattgggagg agttaaaaaa gaggtttagt gaatttaaaa aagataaaaa tattaactgt
      241 tattctatac ctgttgaagc ttccaaaggt tcaaaaagga cagatcttgg tgaaacaata
      301 ctaaactggt gggaatattt tgaacaaaaa agcttagctc agaatattaa ataccaatat
      361 tgcatgttta ctgacatcgc tgattgttat ccatcaattt atactcactc tataacatgg
      421 gccatgtatg gtaaagaaaa agttaaaaat gatgaaaata agtgttcggg ctcacttggc
      481 gataaaatag ataaaaaaat atctggaatg caacacaatc aaacaaatgg tatccctcag
      541 ggttccgttc ttatggattt tgtagcagag atcgttctag ggtatgttga tttgagatta
      601 tcgaaagaat taaaaaaatt agaaaattta aaagattaca aagtactcag atatagagat
      661 gattatagaa tattttcaaa taatcgtaat gatttggaac aaattatgaa agtgttatca
      721 gaagtcttat atgatttaaa ttttaagtta aattctagta aaacaaaatt atcagatgat
      781 attatcatag attctatcaa aaaagataag ctctattggg aagaaattaa ggcaagtata
      841 agaatgaatg ccacaaactt agatggtgat agagaaattc gttatagtat aaatttacaa
      901 aaacatttat tggaaataaa aaagttagca gataagcatc ctaattcagg ccaattgaaa
      961 aaagcacttt ctgagtttaa taaagaacgc attattaaaa tcacttcaaa gccgaacgat
     1021 cttttaccac ttattggtat cctggtagat atcatgataa ataacccggg ttctttgccg
     1081 cagtgtgtct taattatagg gcagttatta aatttcgaaa atgagagtaa ggctagagaa
     1141 atagttgaaa tgattataaa caaatatgct ttaaagccaa atactgaatt tatgatgata
     1201 tggtttcaaa gattgaccac catttttgga gaaatgtttg atagtgtttt atcatccgaa
     1261 tttgtatcta aaatcaaaga tcctgtctct aatcagatgt tttctgtaga ctggctaaat
     1321 gaagatgcta agaaatcttt ttatgaaggg accattaaag acgaagagaa atatattaaa
     1381 atgagtaaga aaatatatat tactgaattt gatgatttta atgattatcc tttttaagtg
     1441 aaaatttaga atgaatataa aaaacattac aatctaattt ttttaaaaaa atgttatatc
     1501 ggaagtaatc tcttgatttc tatactataa gataagaaaa ccactattta acaaggttat
     1561 tcgaacctta ttttaagtta cttattattt aatgtatcga aaaaggtgaa ataggtttgt
     1621 tataataaga attaagtaag acatgaattg tctaagcaaa tgtagcttat ccatttcatt
     1681 aaaatacata tggtgaaaaa acaagttcaa atagtaacta attttttaaa aaactgattt
     1741 ataaaaaata caacacgaat gaggaaaata accaatgaca gaacaaatcg aaaaaaattc
     1801 aaaaagctta tatcaggcac tgtggaattc cgcagatatc ctgcgttcaa aaatggacgc
     1861 taatgaatac aagagctatt tactaggctt ggtcttttat aaatacttat ccgacaacat
     1921 gctgcggtat gtctccgttc tattggagga agaaacggac gacctaaata aagcacaaga
     1981 cctttacgtt gaagcattta aagatgcaga tataaaggat gatctcttgt atgaactaaa
     2041 agatgagttt tcatatacca tcgcacccgc attgaccttt accgcgcaag tggcagccat
     2101 tcatgatgga tcttttcaat tagaagacct ggtacaaggt ttccgtgaca ttgaacaatc
     2161 cagcgaaaac tttgagaact tatttgaaga cattgatctg tattctaaaa aactggggtc
     2221 aaccccacaa aaacaaaata aaaccatcgc agatgttatg aaagaattag aagggttaaa
     2281 catggcgggg catgctggcg atgtcttagg cgatgcctat gagtacttaa ttggccaatt
     2341 cgcatctgaa tccggtaaaa aagccggtga attctatacc ccacaaccgg tcgcaaaatt
     2401 gatgacacaa attgttttac aaggaaaaga agataaaaaa ggcttttctg tttacgacgc
     2461 gaccatgggt tcgggctcgt tacttttgaa cgctaaaaaa tattcccatc aaccgggaac
     2521 catttcttat ttcggacaag agttgaatac gtctacctat aatttggcgc gtatgaacat
     2581 gattttgcat ggggttccca ttgccaacca acacttgcat aatgcggata ccttggatca
     2641 agactggcca acagaagagc caacgaactt cgatggggtg ctcatgaacc caccgtattc
     2701 tgcaaaatgg tcggctgata aaggcttctt agatgaccca cgcttctctg cctatggcgt
     2761 attagcccct aaatcaaaag cggactttgc tttcttatta catggctact atcatttgaa
     2821 agatacgggc gtcatggcca ttgttttgcc acatggggtg ctctttagag ggggcgcaga
     2881 agggaagata cgcaaagcct tgttagaaaa tggtgccatt gacaccgtca ttggcttacc
     2941 tgctaacatc ttctttaata cgtccattcc aacgaccgta attatcttga agaaagaccg
     3001 tgcgaaaaaa gatgtgctct ttattgatgc gtcacaagat tttgaaaaaa taaaaacgca
     3061 aaatacgcta cgagattacc atattgacgc tattttagag gcctataaaa cccgtacaat
     3121 taaagacaag tatgcttatg tcgcagaatt tgatgaaatt gtggagaatg attacaactt
     3181 aaacattccc cgttacgtgg atacctttga agaagaa
//
LOCUS       DANKEF010000140         3203 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000140 DANKEF010000000
VERSION     DANKEF010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.145"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            670..765
                     /locus_tag="PWZ48_002913"
     CDS             670..765
                     /locus_tag="PWZ48_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2262513.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1067..1312)
                     /locus_tag="PWZ48_002914"
     CDS             complement(1067..1312)
                     /locus_tag="PWZ48_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262514.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1657..1941
                     /locus_tag="PWZ48_002915"
     CDS             1657..1941
                     /locus_tag="PWZ48_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262515.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            1944..2360
                     /locus_tag="PWZ48_002916"
     CDS             1944..2360
                     /locus_tag="PWZ48_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262516.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
     gene            complement(2497..3174)
                     /locus_tag="PWZ48_002917"
     CDS             complement(2497..3174)
                     /locus_tag="PWZ48_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262517.1"
                     /translation="MNLVPLKNFNAKQMDLFFALCARMKDRGLKKVRFSFEELKELSD
                     YKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEINKTKKFVEVSVNPD
                     LEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIP
                     EYYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKIS
                     LRNWLEE"
BASE COUNT         1065 a          599 c          500 g         1039 t
ORIGIN      
        1 tccgtttttt catccattcc tttcttcccc ccttacaaat aaataataac ctttaacaac
       61 caactcgtaa ataggttggt tgttattata caaaaggata ttagtactca gataaaaagt
      121 tgttagtact cagataaaaa gttgttagta ctcagataaa aagttgttag tactcagata
      181 aaaagttgtt agtacccttc tgtatccgtt gggagagtaa ggctcaactg ccccctaaaa
      241 agggttctaa aaaatcttta aaaaataaat aaaataaacg cgcgcgcgcg agcaaaattt
      301 cccgccaaaa aatcacccac tttttaaaat aaacaggttc gcgcatgata ttaaactgcc
      361 aatctagctc cctatggtcg ctgtaagaag gagtgatcgc tacgctctca cattttactt
      421 gcagcagtcg cctttaggct ccttcgccaa aaataattca ttagggactc ctttgtggcg
      481 tcctgagagc tcgtctaagc cgttctaaga caagattcga taaaaactat gtccttcgtt
      541 ctaaagcgcc ttacaggcgg tcagggcaag ccctaacaac cccaaggcaa taaaaaactt
      601 aggcttattt ttgctttttt attgtataat tgattgtcgc ctatccgaca gtcttgcgac
      661 tggaaggagg tgtgtttcgt gttcactaac tttatttgct cccttcttgt tgggctttta
      721 agcgctttgt tcagttactg gttgaataac cgaaacaaga agtaaaactt aattaaggcg
      781 actcagccca caaaaaaagc cccagagaat ccgacctctc tggggctttc gtgtgtttcg
      841 tgttcactta tttgcaatca aattataaca gaaataaata tttatttcaa aaatatccta
      901 caaaagtcta aaatccgtaa ttatttctta tatgcaacat ttacaaataa agtcgctctg
      961 agagctcgta tttttcgatt taagacgttt tatttagttt ttggtgtatt tcattggtcc
     1021 aagctaaaaa actcttagaa gcgattttag agccatttaa acccacttaa ttatattctc
     1081 catgaaattc gctccatgcg tcaaaagctg cgtctgaaat tccgccgtct tcgttcacta
     1141 gttgcattaa tttttccaac gatcgttttc tttcgggtgt catattttta tattccaaca
     1201 atttttcgtc tgttaaatcg aacttgattg cttgaattgc ttgctctaat ttttctaagt
     1261 tttgaaaata ttcgtaagga aatttttcca ttttttcttt ctccagtctc acaatttttc
     1321 tagaaataaa atcacttctt tgccgtctat actctcaaaa aaaatgtcgt ttgaaaaact
     1381 aaataaattt ttgatcgtgc atttttaaat ttggttatcg attacacgtg cagcactccc
     1441 ccccgaattt taaagagcat aaggaaacgt gtacccactt tctaaaaaaa gtgggtagat
     1501 aggctgtgcc tatcacgttt ctcagaaaaa aatttgatat cactttttcg ccatcgtggg
     1561 tagcttatgt atagcatttt ggcaaaaaac agctatacaa atatagtatt tgtatggtat
     1621 acataaatta tacataattg caaaaggaga ttttttatgg acattttagt tcggaatatt
     1681 gatccaaagt ttgttgcaac aatagacaaa cgttgccaag aactcacgaa taaaacagga
     1741 gaaaaatgga gtcgaaataa atatctaaaa gttttaatcg aaagtgattt tgaccgtcaa
     1801 cttacgactt acaaaaaaga ccaattcgat ttattgctcg ataaatttat tgctatccaa
     1861 acagaaaata ctcaaatatt aagcgagtat gtctacacaa ataatcaaat cattgaaatg
     1921 cttatcggag gaatgatata accatgagaa aaacttctgt ccaaaaacat gttactttgt
     1981 accctagtca tgttgacgtt ttagattcca tcatcgaaaa aaatccagat atcaataact
     2041 actcggaagc cattcgcttt ttatgtatga gttatgaacc cgataccaac acgaccacta
     2101 ccagcaatca ggcaaaacta aatgctatgg gaaaagaaat ttccatcatg actgaaatca
     2161 tcgcagaatt tgctgattta tatctaaaag atacaggcgt tcttattggc acaaattcta
     2221 aagtatatca agaagtgaaa gaaaaaatcg aagctaaact aaatccatca aatcgaaatc
     2281 gaccaaagaa tcatcaaaaa gaaaccagtc aaacagtcac ttctagaaat ttcgacccaa
     2341 caaattttct aaaacgataa aaaacagata ggagttattc ctatctgttt tttatttaga
     2401 agccccatac aatatgctag aatgtactag caatcttagt cggttgtaag ggacttgtct
     2461 acttgcttaa tgtaaggggg gcgagttctt ttttatttat tcttctaacc agttacgcag
     2521 agaaatcttt ggcaaatctg acgttaggct tgaaaaagta aattctaatt tggcgatttt
     2581 attgcccttt tttgctttta tttttgtgac tttaagatca ttgaagtatt tttttagttc
     2641 tcgtaatgca ggatttaata ctttttgatc aatgttaccc atttgatagt attctgggat
     2701 atccattaag ctcctaaact cttcaatacg aactacataa taaccagtag aacggtactg
     2761 cataagcagt cggaatagag tttttgcata cgtagaacgc aacgacgtga aagcagataa
     2821 ttcaaatcta ctaaattcag ctgttaaacc attaataata tattctaggt ccggattaac
     2881 actgacttca acaaactttt ttgttttatt gatttcaaaa ccagtaaaca aaacaaagta
     2941 gcgaatagaa tcctcatctt cagtacggta agtaagatta agcatatctt tatagagctt
     3001 ttctaaatca ccgacaaagg cttttgtagc agtcatctta taatcactta actcttttaa
     3061 ttcctcgaaa gaaaatctaa cttttttcaa accacgatct ttcatacgag cgcataatgc
     3121 aaaaaataaa tccatctgct tagcattgaa atttttcagc ggtactaaat tcaattcatt
     3181 ttgatatttg actgttaatt ccg
//
LOCUS       DANKEF010000141         3129 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000141 DANKEF010000000
VERSION     DANKEF010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.146"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            8..81
                     /locus_tag="PWZ48_002918"
     tRNA            8..81
                     /locus_tag="PWZ48_002918"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:42..44,aa:Pro,seq:tgg)
     gene            101..173
                     /locus_tag="PWZ48_002919"
     tRNA            101..173
                     /locus_tag="PWZ48_002919"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:134..136,aa:Ala,seq:tgc)
     gene            201..274
                     /locus_tag="PWZ48_002920"
     tRNA            201..274
                     /locus_tag="PWZ48_002920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:235..237,aa:Met,seq:cat)
     gene            288..361
                     /locus_tag="PWZ48_002921"
     tRNA            288..361
                     /locus_tag="PWZ48_002921"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:322..324,aa:Ile,seq:cat)
     gene            385..474
                     /locus_tag="PWZ48_002922"
     tRNA            385..474
                     /locus_tag="PWZ48_002922"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:421..423,aa:Ser,seq:tga)
     gene            487..560
                     /locus_tag="PWZ48_002923"
     tRNA            487..560
                     /locus_tag="PWZ48_002923"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:521..523,aa:Met,seq:cat)
     gene            579..654
                     /locus_tag="PWZ48_002924"
     tRNA            579..654
                     /locus_tag="PWZ48_002924"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:612..614,aa:Asp,seq:gtc)
     gene            656..726
                     /locus_tag="PWZ48_002925"
     tRNA            656..726
                     /locus_tag="PWZ48_002925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:688..690,aa:Gly,seq:tcc)
     gene            740..813
                     /locus_tag="PWZ48_002926"
     tRNA            740..813
                     /locus_tag="PWZ48_002926"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:774..776,aa:Ile,seq:gat)
     gene            838..925
                     /locus_tag="PWZ48_002927"
     tRNA            838..925
                     /locus_tag="PWZ48_002927"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:872..874,aa:Ser,seq:gct)
     gene            947..1018
                     /locus_tag="PWZ48_002928"
     tRNA            947..1018
                     /locus_tag="PWZ48_002928"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Glu,seq:ttc)
     gene            1041..1130
                     /locus_tag="PWZ48_002929"
     tRNA            1041..1130
                     /locus_tag="PWZ48_002929"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1077..1079,aa:Ser,seq:tga)
     gene            1143..1216
                     /locus_tag="PWZ48_002930"
     tRNA            1143..1216
                     /locus_tag="PWZ48_002930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1177..1179,aa:Met,seq:cat)
     gene            1235..1307
                     /locus_tag="PWZ48_002931"
     tRNA            1235..1307
                     /locus_tag="PWZ48_002931"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1268..1270,aa:Asp,seq:gtc)
     gene            1311..1383
                     /locus_tag="PWZ48_002932"
     tRNA            1311..1383
                     /locus_tag="PWZ48_002932"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1344..1346,aa:Phe,seq:gaa)
     gene            1388..1458
                     /locus_tag="PWZ48_002933"
     tRNA            1388..1458
                     /locus_tag="PWZ48_002933"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1420..1422,aa:Gly,seq:tcc)
     gene            1471..1544
                     /locus_tag="PWZ48_002934"
     tRNA            1471..1544
                     /locus_tag="PWZ48_002934"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1505..1507,aa:Ile,seq:gat)
     gene            1556..1627
                     /locus_tag="PWZ48_002935"
     tRNA            1556..1627
                     /locus_tag="PWZ48_002935"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1589..1591,aa:Glu,seq:ttc)
     regulatory      1816..1895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            1893..3086
                     /locus_tag="PWZ48_002936"
     CDS             1893..3086
                     /locus_tag="PWZ48_002936"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDL2262518.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
BASE COUNT          819 a          632 c          796 g          882 t
ORIGIN      
        1 aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa ggggtcgcag
       61 gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag ctcagctggg
      121 agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct ccatttcatt
      181 taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc ggttcatacc
      241 cgggaggtcg ggggttcgat tccctccgcc gcgattttta agagcctgga cctttagctc
      301 agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc tacaaggtcc
      361 attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag ggaacggtct
      421 tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc tcctttttca
      481 tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga aggtcgtagg
      541 ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg taggggttaa
      601 catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc gccatgcggg
      661 tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg attctcgtca
      721 cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg cctgataagc
      781 gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat tggttttggg
      841 gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg aaaccggcgc
      901 gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc cgttggtcaa
      961 gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt acgggtcatt
     1021 aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa
     1081 aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct ttttcatatt
     1141 atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt cgtaggttca
     1201 aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg ggttaacatg
     1261 cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt ggctcggtag
     1321 ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt ctgtcccgcg
     1381 ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt gtcgcgagtt
     1441 cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt tagagcgcac
     1501 gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt ttattggccc
     1561 gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc gaatcccgta
     1621 cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga atgattttaa
     1681 aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga cagttctaat
     1741 gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag aaaactgttt
     1801 tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg gagatgcctt
     1861 gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta tttacatcag
     1921 aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat gcaatcttgg
     1981 atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct gtaacaactg
     2041 gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt caaaaagtcg
     2101 tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt gatggagata
     2161 cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa ggagttgatg
     2221 aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt ggtgctggtg
     2281 accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg cctttaccaa
     2341 tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca aatgaattaa
     2401 cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat caaggacaac
     2461 cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta gataatgaaa
     2521 caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct gaattattag
     2581 atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt ggtcctcaag
     2641 gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt tatgctcgtc
     2701 atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct gccagctatg
     2761 ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa gcagaagtac
     2821 agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat actttcggta
     2881 ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc gatcttcggc
     2941 cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa acagctgctt
     3001 acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac aaagtagatg
     3061 cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc caagtggtgg
     3121 ttgttccag
//
LOCUS       DANKEF010000142         3046 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000142 DANKEF010000000
VERSION     DANKEF010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.147"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1193
                     /locus_tag="PWZ48_002937"
     CDS             <1..1193
                     /locus_tag="PWZ48_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2262519.1"
                     /translation="QFSDETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYE
                     DKVNAAITTNTLPDVITLDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYD
                     GKMYAFGFSESSVGVYYNKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTP
                     AIDMQMASGDEMLTYAYTPFVWSNGGDVVNADGTKAEDYFNDKKSVEALEFIQELVEK
                     GYTTISPIEKGFETGKYAMLLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTG
                     SWQLAMTEKTEKKDASAAFIKFATSTESSRIISLGNSVLPIRNSTVDLIKDEVSEGMQ
                     VLIKQNQISGHARPVVVAYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAID
                     TSLK"
     gene            1236..2102
                     /locus_tag="PWZ48_002938"
     CDS             1236..2102
                     /locus_tag="PWZ48_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDL2262520.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQLGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            2099..2947
                     /locus_tag="PWZ48_002939"
     CDS             2099..2947
                     /locus_tag="PWZ48_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDL2262521.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
BASE COUNT          916 a          534 c          638 g          958 t
ORIGIN      
        1 ttcagttttc tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta
       61 atgatgaata cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg
      121 gcggtggata tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta
      181 ttacgttaga tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag
      241 atgattattt gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa
      301 cctacgatgg caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca
      361 ataagtcgtt gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc
      421 cttggacttg gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc
      481 ctgcaattga tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat
      541 ttgtatggtc gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt
      601 ttaacgataa aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt
      661 acaccacaat cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgctgctga
      721 gcggttcttg gacaatggca gatttagagg aaaactatca agagattgat tatggtattt
      781 tgccttaccc cgtttcagat gcaactgggg agttagtttc accaaccggc agctggcagt
      841 tagcaatgac tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa
      901 ccagtacaga atctagtcgt attattagtt tagggaacag tgttctacct atcagaaatt
      961 caacagttga tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa
     1021 accaaatttc tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat
     1081 tccaacaagc agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag
     1141 cagatagtcg cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc
     1201 taaagggggc caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg
     1261 gctttctagc gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg
     1321 taatttatta tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg
     1381 gcttagaaaa ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca
     1441 cggctaaatt tgttgtttgg attattccgg tccaattagg ggcagcttta ggaatggcac
     1501 ttattatcaa caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag
     1561 tcgtgatgtc cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag
     1621 gtctggttaa tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc
     1681 ccaaacaagc aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga
     1741 tgctgctgtt tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa
     1801 ttgatggttt ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca
     1861 cggcgatttt tattttactt accacattga tttcggcctt taaactaatc attcagccaa
     1921 tggtaatgac tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc
     1981 aaactggatt tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca
     2041 ccatgattgg tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat
     2101 gaccaagaaa tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc
     2161 attattcatt ttcccaatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt
     2221 ctatcaaaat ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt
     2281 taaaacttat caagaagtta tttcccggtt cagtgtggga acttatttat taaacagtat
     2341 tttttatggt ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc
     2401 ctttgcgaag gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct
     2461 gattgtcccg atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt
     2521 agtgaatact cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctattt
     2581 gtttagaaat ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg
     2641 tgcaaatatt gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc
     2701 cacagtaggt gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt
     2761 cttaactgac agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca
     2821 gccagtttat atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat
     2881 tgtttatatc gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat
     2941 aaaataggag tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga
     3001 aaaatgctca aacagtcaat caagaatacc ggaataaatt tcattt
//
LOCUS       DANKEF010000143         2952 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000143 DANKEF010000000
VERSION     DANKEF010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.148"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            89..406
                     /locus_tag="PWZ48_002940"
     CDS             89..406
                     /locus_tag="PWZ48_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HDL2262522.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="PWZ48_002941"
     CDS             complement(497..862)
                     /locus_tag="PWZ48_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262523.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="PWZ48_002942"
     CDS             1070..1552
                     /locus_tag="PWZ48_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262524.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="PWZ48_002943"
     CDS             1749..2639
                     /locus_tag="PWZ48_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HDL2262525.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT         1008 a          460 c          566 g          918 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttagac cgatatgcgg cgcgttctgt gcgccgcata tttttttagt tttcaaagat
     2941 cagaccttgt tg
//
LOCUS       DANKEF010000144         2939 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000144 DANKEF010000000
VERSION     DANKEF010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.149"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..236
                     /locus_tag="PWZ48_002944"
     CDS             <1..236
                     /locus_tag="PWZ48_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262526.1"
                     /translation="VGKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMA
                     TASYIYQLMMFKQARDAYQKEFEKANKEKKLQK"
     gene            438..>2939
                     /locus_tag="PWZ48_002945"
     CDS             438..>2939
                     /locus_tag="PWZ48_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDL2262527.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASEAEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLSKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDFIVAIGGLTAAFGILLPAILAVSYLFGPMML
                     IIGGIIAVIAGVIVAIKNWGAITDWFSGLWKKFTDWLGDTWESIKDGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQTFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWT"
BASE COUNT         1026 a          434 c          664 g          815 t
ORIGIN      
        1 ctgtgggaaa aggatccatg gatatttctg ttattcaagg tgcaaaggct gtgtatattg
       61 cttaccgcca agcgaacatg actgattata tttcattcga tgaatttatc gataaatggg
      121 attttgatat ggctactgcc agctatattt atcaattgat gatgttcaaa caagcacgcg
      181 atgcttatca aaaagaattt gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
      241 tccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
      301 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
      361 caacaagagt gcttatgaag gatggaaaaa ctatgcaata aaccaatccc agaaaaacta
      421 aagaaaggag gtaaaaaatg gctaaaaaga gaacagaagc agaagtaact ttcatagcta
      481 acgatgacgg attgaaatct acgttaaaag aaatcagtgc tgaattaact aaaaatagag
      541 cagaattaaa actagaacaa gctcaattac aacagactgg ttctgaatca gacaagttag
      601 gaagtaaatt atcttcttta gaaaagcagt atgaattaca aagtcaaaaa gttgaagtaa
      661 ctagccaacg tttagccaat gcgaaaaaat attatggaga aaattccacc gaagttcaga
      721 aacttgaaag agaactgatt aatcaacaaa cagcgcaaca acgtttgtca aacgaaattg
      781 ataaaacgag taatgcacta gctcaagcaa aaggcgaaat acagacgtac gagtctacaa
      841 tgcaacagtt ggatagcgaa caaaaaaatg ttcaagctag tgcttctctg attgaatccg
      901 aatacaaaaa atggcaagca actgctggtc aatcagcttc tgaagccgag aaattagcga
      961 aagcccaaga atatgtttct caacaatctg aaaatgcgga gaaaacgata gatatcctaa
     1021 gacgacagtt agaagctaca cagtctgaat ttggcgctac atccacagaa gcaatgcaga
     1081 tggaagcgaa gcttaatgat gctgaacgtg aatttgaaga gttaggacaa gctgctaaaa
     1141 atgtagatac aactaacttg gacgatatcg gaagcaaaat agatatgaat aatttaatgg
     1201 aagcttctga cgttttaagc gacattggcg ataagcttac agaattaggg aaacaagcag
     1261 tggactctgc taacagtgta ggtagttccc agagtaagat acaagctaat tttggtttgt
     1321 ctaaacaaga ggctgaagaa ttaacgaatg tagccagaga catttattat aaaggttttg
     1381 gagaatcgtt agatcagtcc acagatgcat tgattttggt aaagcgtaat ttaggagatt
     1441 taaataatca agatttacaa aatatcacgg aacaagctat ggtcctagaa aacaccatgg
     1501 gcgctgatat ggatgaaacg ttacgtggtg taaatggctt aatggtcaat ttcggcttga
     1561 gtgctcaaga tgcaatggat ttaatggttt cgggtactca aaacggttta gataaaacgc
     1621 acgaattagg cgacaatatg gcagaatata gccaattatg gagtcaaatg ggatattcag
     1681 ctgatgaaac gttcggaatg cttcaaaatg gtttagatgc gggtgcttat aaccttgata
     1741 aagtcaatga cttagttaag gaaatgggaa tatcgttaac agatggtcga tttgagcaaa
     1801 acatggatat gtttagtgaa agtactagaa aagcttttga agagtggaaa aatggcggag
     1861 gaacacaaaa agacgttatt aattccatga ttcaagattt tagcaatatg gatggtcaat
     1921 acgaccaatt aaataaagct tcgacaattt ggtctgcgct tggcgaagat aatgcgatga
     1981 aagttgtcca atctttaact gatgttaacc atacatttga tgatgttagt ggatctgcac
     2041 aaaaaatgaa tgaagattct actactccgt tgcaagagtt gaacgggaaa atagctgaat
     2101 taaaggattc attagctcct ataggcaaca caatcataga tgcactcgaa ccagtaattg
     2161 attttctagg aaagatggct gatgcgttta ataatcttcc acaaccagta caggatttca
     2221 ttgttgctat aggtggtctt acagcagcat ttggcatact gttaccggca atactagccg
     2281 tgtcatattt atttggtccg atgatgctta taattggagg aattatagct gttatagcag
     2341 gcgttattgt ggcaattaag aactggggtg caattactga ctggtttagt ggtttatgga
     2401 aaaaatttac tgattggttg ggtgatactt gggaaagtat aaaagacgga gcctcatcag
     2461 tttgggatgg ggttaaagaa acctggtctg gatttgtaga ttgggttcaa gatatttggc
     2521 aaggagtttc tgattggttt ggagagctat ggagcggatt agttgaagga gcttccaaca
     2581 tctggcaagg agtccaagaa acttggcaaa cattcgttga ttgggtttca aatatttgga
     2641 acggagtcaa agaagtatgg tcgattattt gggcagacat tgtaggaatt gttcaaatac
     2701 catggacatt aataacgtca ttgattcaag ctggtattaa tattatcgtg ggtatttttg
     2761 atgtagctgg acagttattg ggcgcagctt ggcaagctgt ttggacacct atttctgatt
     2821 tccttaaaaa tacttgggat actatgacac aatggataag tatcgcttgg aatgggattg
     2881 taactacatt ccatactata tttgatccag tagtggcatg gtggaatggt atatggaca
//
LOCUS       DANKEF010000145         2867 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000145 DANKEF010000000
VERSION     DANKEF010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2867
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.150"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..733
                     /locus_tag="PWZ48_002946"
     CDS             <1..733
                     /locus_tag="PWZ48_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HDL2262528.1"
                     /translation="VLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVLKILDTLLMI
                     GYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETIHLGVLDNNE
                     ILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLEKHPLIPYTE
                     YTITNALRLKKEIQTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAFSVSMPKYRL
                     TDETKEQIISALKQTKSEIEQRIQK"
     gene            833..1477
                     /locus_tag="PWZ48_002947"
     CDS             833..1477
                     /locus_tag="PWZ48_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDL2262529.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            1484..2491
                     /locus_tag="PWZ48_002948"
     CDS             1484..2491
                     /locus_tag="PWZ48_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDL2262530.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            2507..>2867
                     /locus_tag="PWZ48_002949"
     CDS             2507..>2867
                     /locus_tag="PWZ48_002949"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="4-deoxy-L-threo-5-hexosulose-uronate
                     ketol-isomerase; catalyzes the interconversion of
                     4-deoxy-L-threo-5-hexosulose uronate to
                     3-deoxy-D-glycero-2,5-hexodiulosonate; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDL2262531.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQD"
BASE COUNT          985 a          515 c          640 g          727 t
ORIGIN      
        1 tgtattgatc aaagcctcta aaatactaga ctatttagcg gaaaaccaag atgtctcttt
       61 gcagacgatc gcaaagggtg tgggaatgac ctcttctaca gtcttgaaaa tactagatac
      121 tttgttgatg atcggctacg taaataaaaa ttcagagaaa aactatcgtt taggtgcaaa
      181 gcttattcgc tacgcgaata aaaacattga acaaatcgat ttggtagagt tgactttacc
      241 gtttttagaa cagctgcagc aaaagatcga tgagactatc cacttagggg tattggacaa
      301 caatgagatc ttgtacgtga acaaactaga tccaaaacat caaacgatcc gcatgtcttc
      361 aaaaatcgga atcacccgac cattatacaa ttctgcgatg ggcaaagccg tattagcaga
      421 attcagtgaa gagcagtaca ctgattatct tgaaaagcat ccgttgatcc cttacacaga
      481 atatacgatc accaatgcat tacggttgaa aaaggaaatc caaacggtaa aagaaaccca
      541 catcgcattt gacgatgaag aaatcgaacg tgatattttt tgtataggag cctcaatcgt
      601 gaaaaacgaa caaatcattg gtgcatttag tgtaagtatg ccgaaatatc gtttaacaga
      661 tgagacaaaa gaacaaataa tcagtgccct caagcaaaca aaaagcgaaa tcgagcaaag
      721 aattcaaaaa taatgaaaaa agcgtttgca atttttcttt tctgccgtat aatatgggta
      781 tagatttcca agttataaaa taaaatttct aaataggaaa taggtggaga agatgaaaaa
      841 aattgatatt ttatctcgat tgaaaaacgc cggagtgatt gcggtggtac gcggaaaaag
      901 caaagaagaa gctttgaatg cttgccacgc aatcatcaaa ggtggtctaa ctggtattga
      961 gttgactttt acagtccctc aagcagatca agtaatcaaa gaacttttgt cattctacaa
     1021 agaccaacca gaaatagtga ttggcgcagg cacagtatta gacgctgtca cagctcgctt
     1081 ggctatctta gctggcgcag agtatattgt tagcccttcc tttgatcaag aaacggcaga
     1141 gatgtgcaat ctgtatcaaa ttccttactt accaggatgt atgactatta ccgagatcaa
     1201 aacagcgctg aaaagcgggg tagatatcgt caaactattt cctggaagcg cttatggacc
     1261 aagtatcatt tctgccttca aggcgccgat gcctcaagtc aatatcatgc caactggcgg
     1321 tgtgagtctg gacaatatga aagagtggtt cgatgctggt gtcgtaacag tcggagtagg
     1381 tgggaacctt ttagcacccg cagctacagg agattttgac aaagtaacgg aagtcgcgca
     1441 acagtatgca gcaaaaatga aagaaatcaa gaggtagctg cttatgggaa aagtagtgac
     1501 actcggagaa atcatgttgc gattgtccac agagcaggga acccgtatcg attcaagtga
     1561 acgattcgct gcccattatg gcggagggga agcaaacgta gcaatttctt tagctaatta
     1621 cggacatgaa gcgatttttg ctacaaaagt acctgataac gcattgggaa aagcagtcaa
     1681 aaagcacttg caatgttatg gagtagatac gaaacaggta ttatctggtg gatcaagact
     1741 aggtacttat tatatggaaa ctggtagtgg cgaacgcgcc gcctcagtga tctatgatcg
     1801 tgccggatca agttttgctg aaatggatca tttggaatgg tcgctagagg agttattcca
     1861 aggaatagat atatttcatg tttcaggaat caccccagct ttgtctaaaa aatggcaaaa
     1921 aatgacgaaa caggtgatag aagctgcaaa aaaagctggc tgcctgatca gcttcgatat
     1981 caactatcgt ggaaaattat ggacacagca agaagccagc gaagtaatcc atcagctttt
     2041 acctttagta gatatctgct cagctggaaa aatggatgct ctttatttgt taggtatcga
     2101 gaaagcgcca gaagcagaag aacaacctct aatttattat tatcaaaaaa tgcaggaaaa
     2161 gttcccgaat attcaggtgt tttattcgac caagcgtacc gttcattcag caagtgaaaa
     2221 tgaattgaag gggacattgt ggatgaacca tgaatatgtg gaatcccaag tccatagaat
     2281 caccccaatc gtggatcgag taggtggcgg agatgctttt gcaggtggcg tgctccatgg
     2341 gattttatcc aatatgtctc cgcaagaaat cattgatttt gctacggcag cttctgcctt
     2401 gaagcatacg atccatgggg actgcaacca atttagtcaa gaagaagtcg cacaattttt
     2461 agcatgcggc tctggaaaaa tcattcgata gaaggagtag aagaaaatgc aagaaatgga
     2521 aacacgttat acacacagtc cagaagatat ccgtcattat tcaacggaac aattgagaaa
     2581 agaattttta gtagaaaagg tatttgaacc agggaaaatc agtttgactt atacgcacaa
     2641 cgatcgaatg atctttggtg gagtgacacc tactgaaaaa gaattagaaa tcacattaga
     2701 caaagaacta ggtgttgatt acttcttaga gcgtcgtgaa ctaggtgtga tcaatatcgg
     2761 tggaccagga ttcatcgaga tcgatgggca aaaagaagaa atgaaaaaac aagacggata
     2821 ctatatcgga aaagaaacac gacatgttgt attttcttct caagatg
//
LOCUS       DANKEF010000146         2841 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000146 DANKEF010000000
VERSION     DANKEF010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.151"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..236
                     /locus_tag="PWZ48_002950"
     CDS             <1..236
                     /locus_tag="PWZ48_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262532.1"
                     /translation="MGKGSMDITVIQGAKAVYVAYRQANMVDYISFDEFIDKWDFDME
                     VAVAVYSTMMFKQARDAYQKEFEKANKEKKLQK"
     gene            239..421
                     /locus_tag="PWZ48_002951"
     CDS             239..421
                     /locus_tag="PWZ48_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262533.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            439..>2841
                     /locus_tag="PWZ48_002952"
     CDS             439..>2841
                     /locus_tag="PWZ48_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2262534.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNDIDKTSKALAQSKGEFKTYESTMKDLDNEQQHLKSSASLVESEYKKWQ
                     ATAGQSATESEKLAKAQEYVGKQSDIAEQKIEVLKKQLDATQKEFGETSTEALEMKTK
                     LNDAEREFEELSNAAKNVDTSTVDDIGKKLDMGNLMEATDHLSVIGDKLIDVGSKSIE
                     AAGKAQAMQAQFKQVFGSLEGEAQDAVEGMAEEFGMLPNTIKPVFIQYTSMFKGLGYD
                     TKEAMELAGDSTQLAADAAAFYDKSMDDASESLNSFIKGNYEGGEQIGLFANDTQMAA
                     YAVKHNLIPATEGAKKASEESLLAVEKAQSKYADAIKKHGEGSLEAREAALKLKDAQD
                     KINEELGPQTQKWSDLDEATKQAVRVQYAEDMQKLAGATGQASRESDGLENQMTRAKQ
                     ALEDFYASLGEDILPVFIKGLQAGAKALQGLAEWWSKLDGPMKNFILALGGILALLST
                     LAPVITAVVTIVGTFGSTVLLPIIGIIAGVAAVIAIVITAFQNWGAITDWFSDLWKKF
                     TDWLGDTWESIKESASSVWDGVKETWSGFVDWVQEIWQGVSDWFGELWSGLVEGASNI
                     WQGVQETWQAFVDWVSNIWNGVKEVWSIIWADIVGIVQIPWTLITSLIQAGINIIVGI
                     FDVAGQLLGAAWQAVWTPISDFLKNTWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNG
                     IWT"
BASE COUNT         1012 a          417 c          650 g          762 t
ORIGIN      
        1 cgatgggaaa aggctctatg gatattacag ttattcaagg tgcaaaagcc gtatatgtag
       61 cttatcgtca agcgaacatg gtcgattaca tttcattcga tgaatttata gataaatggg
      121 attttgatat ggaggttgca gtagctgtat acagtactat gatgttcaaa caagcacgtg
      181 atgcctatca aaaagaattc gaaaaagcaa ataaggaaaa aaagcttcaa aagtaaaaat
      241 gccaaagctc ttagttgaaa cgtgggtcga tgtctattcg atgttgaccg acgttttttc
      301 tatgccttca gatttggttt taagcgatat ctgtttagat gacattttgc aaatggctta
      361 caacaagagc gcttatgaag gatggaagaa ctatgcaata aatcaatccc agaaaaacta
      421 aaggaaagga ggtaaaaaat ggctaaaaag agaacggaag cagaagtaac tttcatagct
      481 aacgatgacg gattgaaatc tacgttaaaa gaaatcagtg ctgaattaac taaaaataga
      541 gcagaattaa aactagaaca agctcaatta caacagactg gttctgaatc agacaagtta
      601 ggaagtaaat tatcttcttt agaaaagcag tatgaattac aaagtcaaaa agttgaagta
      661 actagtcaac gtttagccaa tgccaaaaaa tattatggag aaaattccac cgaagttcag
      721 aaacttgaaa gagaactgat taatcaacaa acagctcagc aaaggttatc taatgatata
      781 gataaaacga gtaaggcatt ggctcaatct aaaggtgaat ttaaaacgta tgagtcaact
      841 atgaaagact tagataatga gcaacaacat ctaaagtcaa gcgcatcatt agtagaatcc
      901 gaatataaaa aatggcaagc aactgctggc cagtctgcta cagaatctga aaaattagca
      961 aaagcacaag aatatgtcgg caaacagagt gatattgctg aacaaaaaat cgaagtgcta
     1021 aaaaaacaat tagatgccac acaaaaagaa tttggagaaa catctaccga agctttagaa
     1081 atgaaaacta agcttaatga tgcggaaaga gaattcgaag aattaagtaa tgctgctaaa
     1141 aacgttgata cctccacagt agatgatatc ggcaaaaaac ttgatatggg taatttaatg
     1201 gaagctactg atcacttgtc agtgatcgga gataagctta ttgatgtagg tagtaagtct
     1261 attgaagcag ctggaaaagc acaagctatg caggcccaat ttaaacaagt ctttggctct
     1321 ttagaagggg aagcacagga cgccgttgag ggaatggctg aagaatttgg aatgttacca
     1381 aatacgatca agcctgtttt tatacaatat acgtcaatgt ttaaaggact tggatatgat
     1441 accaaagaag ctatggagtt agctggtgat agtactcagt tagcagcaga tgcagcagct
     1501 ttttacgata agtctatgga tgatgctagt gaatctctta attcatttat aaaagggaac
     1561 tacgaaggtg gggagcaaat aggtttattt gctaatgata ctcaaatggc agcttatgct
     1621 gttaagcata atttgatacc agcgactgaa ggagcaaaaa aagccagtga agagtcattg
     1681 ttagctgttg aaaaagcaca atctaagtat gctgatgcta ttaagaaaca tggtgaagga
     1741 tctttagaag caagagaggc tgctttaaaa cttaaagatg cgcaagataa aataaatgaa
     1801 gaattaggcc cacaaacgca aaaatggtct gatttagatg aggctaccaa gcaagcagtt
     1861 cgagttcaat atgctgaaga tatgcaaaaa ttagccggtg ctacaggtca ggctagtagg
     1921 gagtctgatg gtttagaaaa ccaaatgaca agagcaaagc aagcgttaga agatttttat
     1981 gcctcattgg gtgaagatat actgcctgtg tttatcaaag ggttgcaagc aggagcgaaa
     2041 gctttgcaag gattagccga atggtggagt aaacttgatg ggccaatgaa aaatttcatt
     2101 ttagctcttg gaggaattct agcattatta agcacattag ctcctgttat aaccgcagtt
     2161 gttacgatag ttggcacatt tggttctaca gttttgctgc caataatagg aattatagcg
     2221 ggcgttgcag ctgtaatagc gattgtaatt acagcgtttc aaaactgggg cgcaatcact
     2281 gactggttta gtgatttatg gaaaaaattt accgattggt taggtgatac ctgggaaagt
     2341 ataaaagaaa gtgcctcatc agtttgggat ggagtcaaag aaacctggtc tggatttgta
     2401 gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt atggagcgga
     2461 ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca agcattcgtt
     2521 gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat ttgggcagac
     2581 attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca agctggtatt
     2641 aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc ttggcaagct
     2701 gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac acaatggata
     2761 agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc agtagtggca
     2821 tggtggaatg gtatatggac a
//
LOCUS       DANKEF010000147         2810 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000147 DANKEF010000000
VERSION     DANKEF010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.152"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            803..2680
                     /gene="ltrA"
                     /locus_tag="PWZ48_002953"
     CDS             803..2680
                     /gene="ltrA"
                     /locus_tag="PWZ48_002953"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2262535.1"
                     /translation="MTKETSPKDRKLRHAEYYGMTEIFDKIYSKSLKGDNFKNLMQII
                     ISDNNIVLAYRNIKRNHGSVTRGEDGVTIKDIENLNRNEFVEIVRKRFAHYNPRKVRR
                     VEIPKYNGKLRPLGIPSIWDRIAQQCILQVLEPICEAKFNNHSYGFRPNRSAEHAIAD
                     CMARMNQSHMEYVVDVDIKGFFDEVNHTKLMRQIWSMGIRDKQLLLIIRKMLRAPVVL
                     PNGKLIYPTKGTPQGGILSPLLANINLNEFDWWIANQWEEKDAKELKHHFRKNGAWDK
                     GNIYHKLRKSTTLKEIYLVRYADDFKIFCRNYNEAKKVFYATKMWLQERLKLPISEEK
                     SKITNLSKEESEFLGFTMKLVTKGNKKVVNTHIGTKAQSMIKVKLKKQIKRIQKSPNS
                     KDTFIYIGVYNSMVIGIHNYFKIATHCSVAMRKIARELRPSFEIRLREQGISKEGQYG
                     KYEGYKPYLRSGQIRYIRGFPILPVGYIQHRHPMCKKRNINKYTEEGREAIHKKQQTV
                     EEWKISWLCSNPVLSKRATIEFADNRISKFIAQKGRCAVTGEELILSEMHCHHIIPYH
                     ESKSDSYENLVIVTEEVHRVIHATQSETIEELLKYLKLNPKQKEKLNELRLKVGNEEI
                     S"
BASE COUNT         1076 a          438 c          620 g          676 t
ORIGIN      
        1 taaagaaaaa caaatggaag aacaagactt caaacgcttc ttggcgatga ttgatgagtg
       61 ccataatggt gcgacctgtt ctgcagtgaa aagctgtggg ttgcaagtag tctaaaaact
      121 attgtaataa ctcttataac gagaagtcaa atgagtgggt aacgccacaa agggcttcca
      181 ctgatactcc gacatgcaac aagtagttta tgcagaccaa cgttgtgtga agctcggtga
      241 agtcggctga aggataccgt aaggtaaaag aaatagtgaa taccacgaaa gttctctaga
      301 ggtaaagggt gtatccgata agtcgggtgt ccaaaaaaac ttaatatggt gagtatgtac
      361 aaacgtactg acgaactagc gaatgtactg tccgaaaggc atagctccta gaaatgggag
      421 agacagtgag caaaaaatca ctcattgtgc taacttgtgg ttgagtaaaa gtcgttgtta
      481 tgaaagacca tgctgcgtta caggcgtagg agaagttagg gggcaaaagc tagcatctaa
      541 gttaaggcat gtggatagat ataagggaac aggggaagcc atcaacatgg agagtgcata
      601 ctcgatgaag tggtagtaag aaatatgtga atgacaaata acttacttta gtgatggtga
      661 aagtagagtc acgactgatg aaggttctgt aatggaactg gaggaacagg ctcgattcgt
      721 ctgtaaaagt catgaaaccc gttcaaccat gggattctcg tatgactaaa aaggtcacga
      781 gccaattgga gcgtgataaa ccatgacaaa agaaacaagt ccaaaagata ggaagttgcg
      841 gcatgcagaa tattatggca tgacggaaat cttcgataag atatactcca aaagtttgaa
      901 gggagataat ttcaagaatc taatgcagat tatcatttct gataacaaca ttgtcctagc
      961 atatcgtaat atcaagagaa atcacggtag tgtgacaaga ggagaagatg gagtaacgat
     1021 taaagatatt gaaaatctca accgtaatga attcgtagaa attgttagga agcgattcgc
     1081 acattataac cctcggaaag taaggcgtgt agaaattcct aaatataatg gtaaattaag
     1141 accgttgggt attccatcaa tatgggatag aatcgcgcag caatgcatct tacaagtatt
     1201 agaaccaata tgtgaagcaa aattcaataa tcacagctat ggtttccgcc caaatagatc
     1261 agcagaacac gcaatagcag attgtatggc ccgaatgaat caaagtcaca tggaatatgt
     1321 ggtagatgta gatatcaaag gtttcttcga cgaagtcaat catactaaac tcatgagaca
     1381 aatctggtct atgggtatac gagacaaaca gcttctctta atcattagga aaatgttaag
     1441 agcacccgta gtacttccaa atgggaagtt gatataccca acaaaaggaa caccacaagg
     1501 cggaatactt agcccattac ttgcgaacat caatctcaac gaatttgact ggtggatagc
     1561 aaatcagtgg gaggaaaaag atgccaaaga gctaaaacat cattttagga aaaatggagc
     1621 ttgggataaa ggtaatatct atcacaagct cagaaagagt acaactctaa aagaaatata
     1681 cctagtgaga tacgcagatg attttaaaat tttctgtaga aattataatg aagcgaaaaa
     1741 agttttctat gcaactaaaa tgtggttaca ggagcgtcta aagttaccaa tatctgaaga
     1801 aaagtcaaaa atcacaaatc tttcaaaaga agaaagtgaa tttttaggct ttacgatgaa
     1861 attagtgacc aaaggtaaca aaaaggttgt taatacgcat ataggtacaa aagcacaaag
     1921 catgattaaa gtgaaactaa aaaaacaaat caaacgaatt caaaaaagtc ctaatagcaa
     1981 agataccttt atctatattg gagtctataa cagtatggtt attgggattc ataattactt
     2041 caaaattgcg actcattgta gtgtagcaat gcgaaaaatt gcaagagaac tcagaccaag
     2101 ttttgaaata cgactacgag aacaaggaat ctcaaaagaa ggacaatacg gaaagtacga
     2161 aggatacaag ccttacctac ggtcaggaca aatacgatat ataagagggt ttccaatatt
     2221 accagtaggg tatattcaac acagacatcc gatgtgtaaa aaaagaaata ttaataaata
     2281 caccgaagaa ggtagggaag ccattcataa gaagcaacaa accgtagaag aatggaagat
     2341 atcgtggttg tgtagcaatc cagtgcttag taagagagca acgattgaat ttgcggacaa
     2401 tcgaatttca aaattcatcg ctcaaaaggg aagatgtgca gtaacaggtg aggagcttat
     2461 cttatctgaa atgcattgtc atcatattat cccttatcat gaaagcaaaa gtgatagcta
     2521 tgaaaaccta gtgattgtta ctgaggaggt tcatcgggta atacacgcta cacaaagtga
     2581 aacgattgag gaacttctaa aatatctaaa attaaatcca aaacaaaaag aaaaactcaa
     2641 tgagttgaga ttaaaagttg gtaacgaaga aatatcttaa taaatatttg taggacggga
     2701 aaacagaatg tcttttatag acgagggaac gccgtatgcg gtgaaagtcg cacgtacggt
     2761 gtggactggg ggaaaagacg gagataatat caaactctta cctatcagta
//
LOCUS       DANKEF010000148         2777 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000148 DANKEF010000000
VERSION     DANKEF010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2777
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.154"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..693
                     /locus_tag="PWZ48_002954"
     CDS             <1..693
                     /locus_tag="PWZ48_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262536.1"
                     /translation="FVLFNQFKIDGKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQF
                     NELESSYSKTMFRLLKQYRTKGIANFSKEDFHELLDIPKSYRESDINKKIIKPIRQEL
                     TAIFKGLTIQKRYGKGRGKPVIGYQFTFKPEIKKSDDFNKHAPTKEEQNKALELANQQ
                     VQSEKEPTSLQEKIKEQKRKLELLQQLAELERKQNTPSKKINKQIADNNSDITPEFKE
                     NLLDNLQNLFKE"
     gene            703..1191
                     /locus_tag="PWZ48_002955"
     CDS             703..1191
                     /locus_tag="PWZ48_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2262537.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNITYLKKE
                     AVTTITKVLSGNESGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQIALETQ
                     QKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQNKEKKWWQF
                     WQ"
     gene            1487..1768
                     /locus_tag="PWZ48_002956"
     CDS             1487..1768
                     /locus_tag="PWZ48_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262538.1"
                     /translation="MDILVRNIDPKVISEIDRRCQELTTKTGKKWSRNAYLKVLIEND
                     FDQALRHYKKDQFDLLLEKFIDIQAKNTETLNDYVHTNNQLIETLIGGI"
     gene            1770..2186
                     /locus_tag="PWZ48_002957"
     CDS             1770..2186
                     /locus_tag="PWZ48_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262539.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPNTNTTTTSNQAKLNAMGKEISIMSEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSRNFDPTNFLKR"
BASE COUNT         1123 a          497 c          410 g          747 t
ORIGIN      
        1 tttgttttat ttaatcaatt taaaatcgat ggaaaagctg atattccttt tgctgaaata
       61 caagtccatg agaaagcctt acccttactt aataatcttg aagaatgggt tcgatattct
      121 ctccaacaat tcaatgaatt agaaagcagt tattccaaaa ccatgtttag attattaaaa
      181 caatacagaa ccaaaggaat tgccaatttt tcaaaagaag actttcatga attacttgat
      241 atccctaaaa gttatagaga aagtgatata aataaaaaaa ttataaagcc tattcgccaa
      301 gaactaaccg caatcttcaa aggtttgaca atccaaaaga gatacggaaa aggacgggga
      361 aagcctgtca ttggctatca atttacattt aaacctgaga taaaaaaatc agacgacttt
      421 aacaaacatg ctccaaccaa agaagaacaa aataaagctt tggaactagc taatcaacag
      481 gttcaatctg aaaaagaacc tacttcatta caagaaaaaa tcaaagaaca aaaacgaaaa
      541 ttagaacttt tacaacagtt agcagaatta gaaagaaaac aaaatacccc aagtaagaaa
      601 attaacaaac aaatagcaga taacaattcc gatattaccc cagaatttaa agaaaactta
      661 ctagataatc tccaaaactt atttaaagaa taaggggttt ttatgacaga taaaactatc
      721 aaagaattag cagatattct aggtgtttca aaagataaag tgaagtatca actgaggaaa
      781 ttacctagta attttaccta caaaaaagga aatattacct acctgaaaaa agaagcagta
      841 acaacaatta caaaggtttt atcaggtaat gaatcgggta acttacctag tgaattaccc
      901 gattcattac ctaccgatta cctactcaag gagattgaaa ctaagaataa tcatattgaa
      961 gaaaaagata ggcaaattga aaaacttcaa atagctttag aaacgcagca aaaattactc
     1021 gaccagcaac aacaactcac cctacaagcc aatcaacaaa tcaaaaaact tcacgaacaa
     1081 ctccagctaa cctatgcaga agaatcttca gaaaaagaac ccctttctgt tactgaagaa
     1141 actcaaaaag gacaaaacaa ggagaaaaaa tggtggcaat tctggcaata acccaacaat
     1201 ttttattcat aaaaattgtt gggttacttt ctttttttgc ttttacaagc atagcgacat
     1261 gatctcacgt cgctattttt taaagagcat aaggaaacgc ggtatcactt tcttacgaaa
     1321 ggtgctaggt aggctgtgcc taccacgttt ctaagaaaaa aatttgctat cactttttcg
     1381 tcattgtgga tagcttatgt atagtgtttc ggcaaaaaac agctatacaa atactatatt
     1441 tgtatgatat acataaatta tacacaaatg cgaaaggaat tcttctatgg acattttagt
     1501 tcggaatatt gatccaaaag ttatttcaga aatagataga cgttgccaag aactcacaac
     1561 aaaaactggc aaaaaatgga gccgtaatgc gtacctaaaa gttctaatcg aaaacgattt
     1621 tgaccaggca cttaggcatt acaaaaaaga tcaattcgat ttattactcg aaaaatttat
     1681 cgatatacaa gcaaaaaata ctgaaacatt gaacgactat gttcatacaa acaaccaact
     1741 cattgaaaca cttatcggag gaatataaca tgagaaaaac ttctgttcaa aaacacgtta
     1801 ccctttatcc cagtcacatt gacgttttag attccatcat cgaaaaaaat ccagatatca
     1861 ataactactc ggaagccatt cgttttctat gtatgagtta tgaacctaat actaatacga
     1921 caactaccag caaccaagca aaactaaatg cgatgggaaa agaaatttct attatgtccg
     1981 aaatcattgc agaatttgct gatttatatc taaaagatac tggtgttctt atcggtacaa
     2041 attctaaagt atatcaagaa gtgaaagaaa aaattgaaaa tcaactcaat ccctcccgac
     2101 gaaatcaacc caaaaatcgt caaaaagaaa ccaatcaaac attcacttct agaaattttg
     2161 acccaacaaa ctttctaaaa agatgaaaaa gaaaccgaaa attttttcta tttctcagtt
     2221 tcttttttag ggggcagtct tccagcaaga tttgcaagtt accggtaact tgcacaaaaa
     2281 aagaaaaatc cgcttgtgcg aatccttctt tttgcatttt ggggcgctgc cccaatctcc
     2341 aaatatcggt tatttaatag cgtggtaact caaaaaaata gaacgtactc tgaaataaaa
     2401 aaagactgct ctaacgagca gccaataaaa aataatgagc gtttattctt gaaaaagcag
     2461 cgcgcttatg aagcgctgcc aaaaatttac catattttat gaaaaataac aagtttctaa
     2521 aaaggaatcg gttaagattc acaaagaatc gtaaacagac aatagcggaa aatcaagctt
     2581 ccgaacgtac cttttagcta gaccaatgaa aaatcacaaa gtgagcccct gtatcggttg
     2641 atacaagggc tacttggcat actcgacatt atgtctacta gctcgataaa taacgattat
     2701 gttcccaaaa caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt
     2761 taattatagg tgtatat
//
LOCUS       DANKEF010000149         2763 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000149 DANKEF010000000
VERSION     DANKEF010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.155"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1275
                     /locus_tag="PWZ48_002958"
     CDS             <1..1275
                     /locus_tag="PWZ48_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HDL2262540.1"
                     /translation="WGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAIEKDGKLYL
                     IYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDFRDPKVFQR
                     KEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECPDLFELDGK
                     EVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLDFYAPQTCE
                     NEAGQRIMTAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPVSSIYQVID
                     PQERIEWAKTEENRFLLKGLVYDQHYLRLEINLKQATKLELHYAKGPHHTLLLNYDVE
                     RECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIELFLNDRLTM
                     STTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            1292..2164
                     /locus_tag="PWZ48_002959"
     CDS             1292..2164
                     /locus_tag="PWZ48_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2262541.1"
                     /translation="MIYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
     gene            complement(2398..2763)
                     /locus_tag="PWZ48_002960"
                     /pseudo
     CDS             complement(2398..2763)
                     /locus_tag="PWZ48_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          822 a          520 c          629 g          792 t
ORIGIN      
        1 tggggccatg ctaaaagcaa ggatctaatt cactgggagc atttgccagt agcactggca
       61 ccaggtgaaa gctatgacaa ggaaggctgt ttttccggca gtgccataga aaaggatggc
      121 aagctgtatt tgatttatac aggacacaat gttgtggaag ggcaagttcg acaagttcag
      181 tgtttggcag tttcagaaga tggcattcac tttgaaaaat atgccggaaa tccaatcatc
      241 gcagaagatc acttagcttc tgttgcaacg accgaagatt tccgcgatcc caaagtgttt
      301 cagcgaaaag aagactattt tactgtagtc gctactaaaa ctgcagatgc tcgtggacaa
      361 attgtgatgt tccattccaa agatttacta acttgggagt tttattccgt attgctggag
      421 ggaactccag aacaaggcat tgtatgggaa tgcccagact tgtttgaact agatggcaaa
      481 gaagtcttga ttctttctcc tattcaaatg actccacaag gcaatgctta ccaaaatacg
      541 aactcaactg ttgctttcat tggtactgtc gattggcaaa caggcaaatt tctagtagag
      601 aattttcatg aaatcgatgg cggtttagat ttttatgccc cccagacctg tgaaaacgaa
      661 gcaggccaaa gaataatgac tgcttggatg caaatgtggc agcgtaaaat acctacgaat
      721 gatctgggcc atggttggtc aggttccatg accttgccta gagagctgag ggtggctgat
      781 aatcaattgt tgcaacggcc agtttcatca atttatcaag taattgatcc gcaggaaagg
      841 attgaatggg cgaagactga agagaatcga ttcttactga agggcttagt atatgaccag
      901 cactatttac gcttggaaat caatttaaaa caggcaacga aactagaact tcactatgcg
      961 aagggacccc accatacatt actgcttaac tatgatgttg aacgggagtg ctttactgct
     1021 tccagggaac acatgccgta tgatttatca ggagctgaaa aggaagtgtt gaaaaagcgc
     1081 agtgtgacga ttcctttgga aaataattgt ctaaccatcg aaattttccg tgatacttca
     1141 gctattgagc tttttctaaa tgatcgtctg actatgagta caacttttta tgagacagaa
     1201 aaaggacagg attttctttt gctggcagac agtgaagtaa ccttttcatt ggaaagcggc
     1261 ttaattaatc tatagaaggg agagctcagt gatgatttat ggtgcaattg aagcaggcgg
     1321 cacaaaattt gtgtgtgcca ttggcgatga agaaatgacg atcaaggaaa gagtgagctt
     1381 tccgacaacg acacctgagg aaacaatgcc tttagtcatt gacttcttca aacaatacca
     1441 agcagattta gcagggattg ggattggctc atttgggcct atcgatatcc atcgcgattc
     1501 cgccacttat gggtacatta cctcgacccc gaaattggca tggcaaaatt ttgatttcat
     1561 cggtaccatg aaaaaagaat ttccgatccc gatttcttgg acaacagatg tcaatgcggc
     1621 tgcctatggt gagtatgtct tcggcagtgg caaaggattg agcagtgtcg tttactatac
     1681 gatcggtacg ggtgttggcg gcggtgcctt acaagaaggg cgctttatcg aagggtttag
     1741 tcatccagaa atggggcata tgctcgtcgt ccctcatcca aaagatgact ttgctggcag
     1801 ctgtccattt catggcaact gtttagaagg gatggccgct ggacccgcca tcgaaaaacg
     1861 cttagggaaa aaaggacaag aggttgcaga agatgatcct tattgggaaa tcgaagcctc
     1921 ctatattgct caatgtgcct acaatacaac cttgatgttt tctccagatg tgatcatttt
     1981 tggcggtggt gtgatgaaac aacgccatat gctgcaaaac gtccacgacg catttgcaaa
     2041 attggtcaat gggtatgtcg aaacacctga gttaagcaaa tatatcgtga ccccagcatt
     2101 agaagacaac gctggaacgc tcggttgttt ggcattggct cgagaagcgg tcttacattc
     2161 ataaacgaaa aaaggctctt cgacatttag tgttggtaga gaaagataca actgaaaatt
     2221 tagtcataac cagttaggca gagtgcttaa ctggtttttg ttttacttgg aagatcaagc
     2281 cttgaatgag ataaattctt gctcgaaagt tatggaaatt tcgatagccg taggctactt
     2341 gtattgtgca atagtttttt acaaaaaaaa ggggtgcgga tatttttttg gaccttttta
     2401 acaaatatat ctttgcttct tccgatattg aagagggctt agccctccta aagataactt
     2461 aattcgttga ttattatacc aatgaatata attatccaat ttgtttataa actgattgat
     2521 tgttgtattc ttccagtcac gttgataaaa catctcgttt tttaatcgtc caaagaaacc
     2581 ttcgcacgct gaattatctg gtgaataacc ctttttagac atagagtgaa gcaagttgtc
     2641 tttgttccaa tagtccaact aacaatagca ccatcgtaac aatcgacaag tggtgataag
     2701 tagattttcc ctgcgggaat tctaaattca gtaatatctg ttaaccattt ttcatttggc
     2761 ttc
//
LOCUS       DANKEF010000150         2762 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000150 DANKEF010000000
VERSION     DANKEF010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.156"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..360)
                     /locus_tag="PWZ48_002961"
     CDS             complement(<1..360)
                     /locus_tag="PWZ48_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDL2262542.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLL"
     gene            545..2491
                     /locus_tag="PWZ48_002962"
     CDS             545..2491
                     /locus_tag="PWZ48_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HDL2262543.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT          922 a          446 c          459 g          935 t
ORIGIN      
        1 gagtaagaat agattcccct ctatttcata tgcagaaccg gaataagcgc cgtggctatc
       61 tcgttttgtg tctggaagaa gttctgctcc tgttacatga aaatgaacta aatcatctga
      121 ttcattgtgt acccattctt tcaacccatg agcagctcca aatggccaat tttgataaaa
      181 aagcgtatat tttccgttaa aaaaagaaaa accattagga tcgtttaaca atcctgtttt
      241 aggttcgata tgatagctag cacgccaagg tgatcgtttt acattatcct taattttatc
      301 catctcatct ttagtccaat cagaataagc acgatatcgt atttctcgag gtaagttcat
      361 attttactcc ttcaatttat ctttttacaa ctagtatggt aaacgttttc cgttgaaaaa
      421 tcaatcattt catttaaaaa aataaactat atgacaaacg tttgacatat ttttaagata
      481 tgctaaaata aatttgcatt tcagtgaacc gttttcacgt aaaaataaaa aaggagtgat
      541 tcgaatgaat tacagtaaag tagcttccga cgtaatacaa gcggtcggaa aagataacct
      601 tattgcagct gcacactgcg ctacacgtct tcgtctcgta ctaaaagatg acacgaaagt
      661 gaatcaaaaa gctttagacg aaaatccaga tgtgaaaagc acatttaaaa ttgatggaca
      721 atatcaagtg attatcggtg caggtgatgt taatttcgtt tatgacgaac ttatcaaaaa
      781 aactggactt tcggaaattt caacagatga tttaaaacaa attgctagta aagataaaaa
      841 attcaacccc attatggcat taattaaatt attatcagat atttttgttc caattatacc
      901 tgcccttgtt gcaggtggtt tattaatggc attacaaaac tttttaacag ctgctggact
      961 ttttggacca aaatcaattg aagaaatgta tcctgcgatt gaaggtattt cctcaatgat
     1021 ccaactgatg tccgctgcac cattcatgtt cttaccaatt ttagtaggta tttcagctgc
     1081 taaacgtttt ggagcaaacc aatttcttgg tgctgcaatt ggtatgatca tgacaacacc
     1141 agatcttggt ggctcaactg aatattggaa tatttttggt tatcatgttt cacaaacaaa
     1201 ctatgcttat caagttattc cagttttagc agctgtttgg atattatcta tattagaaaa
     1261 attctttcat aaaaaattgc catcatctat tgactttaca ttcacgccat tattatcagt
     1321 aatgattact ggatttttga ctttcacagt tattggccct gtaatgttat tgttgtctaa
     1381 tggaattaca gacattattg tttggctata taacactact ggatttattg gaatgggtat
     1441 ttttggagga acatattcac ttatagtcat gactggttta catcaatcat ttccagctat
     1501 tgaaactcaa ttactttcag cgtggacaaa tggtatcgga catggagact ttatctttgt
     1561 agttgcatct atggcaaacg ttgctcaagg tgctgcaaca tttgccattt ggttcttaac
     1621 taaaaattct aagacaaaaa gtcttgcttc ctctgcgggt gtctcagcac ttcttggaat
     1681 tacagagcct gccttgttcg gagttaactt aaaataccgc tttccattct tttgtgcttt
     1741 aattggttca ggaattgcta cagcagtagc aggattactt aaagtagttg ctgtttcact
     1801 tggttcagca ggtttcttgg gatttctttc aattaatgca acatctattc cattttacgt
     1861 attgtgtgaa ttgattagct ttgtagttgc ttttgcaatt acttacttct acggtaaaac
     1921 aaaagctgct ggtatcttta aagcagaaga aattgctgaa caaacaccag ttaattcttc
     1981 aaaagtagca acaacagatc caatatctac tgatgttgaa tcaacacaaa caaaagtttc
     2041 atcagaaaca attgttagtc ccatttctgg taaagtgatt agtctttctt cagtaaatga
     2101 tccagtcttt tcatcagaag ctatgggaaa agggattgcc attaaaccaa atggaaacac
     2161 ggtccattct ccagtagatg gtactattca actcacgttt gaaactggac atgcttatgg
     2221 tattaaatca aataatggtg ctgaagttct aattcatatt ggtattgata ctgtatcaat
     2281 gggaggaaaa ggatttacac aaaaagttac tactaatcaa aaagttagaa aaggcgatat
     2341 tcttggagca tttgaccgtt caatgattga gaaagaaggt cttgatgata caacaatgat
     2401 tattgtgacc aatacttcgg attattcaga agtaaatata gtagctgaaa atacagtcac
     2461 tgagggtact gatttacttg aaatcaaata aatgtatttc cctgagtaaa gatggaattt
     2521 tctttccaaa atcaaataat atagattaat caaatatttg agaataacat tttatttaat
     2581 cttttcatat atcaattgta tgaaaaatta ttagaatttt aaaaaatgat aaaaattcaa
     2641 aaagaaaaaa tagtagttat ttacttgtca tgttactaaa taaaaaaata atttaaatat
     2701 gatatatagt catttattta gtgatttcat tctaaaataa agggagaaat gtctatgcta
     2761 ta
//
LOCUS       DANKEF010000151         2722 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000151 DANKEF010000000
VERSION     DANKEF010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2722
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.157"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(127..597)
                     /locus_tag="PWZ48_002963"
     CDS             complement(127..597)
                     /locus_tag="PWZ48_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262544.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            684..1382
                     /locus_tag="PWZ48_002964"
     CDS             684..1382
                     /locus_tag="PWZ48_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HDL2262545.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1560..1901
                     /locus_tag="PWZ48_002965"
     CDS             1560..1901
                     /locus_tag="PWZ48_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262546.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1894..2565
                     /locus_tag="PWZ48_002966"
     CDS             1894..2565
                     /locus_tag="PWZ48_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HDL2262547.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETELFPVL
                     DYRNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPRIDTKKLNMLET
                     ILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCALMERAIAAKRK
                     ELDKK"
     gene            2571..>2722
                     /locus_tag="PWZ48_002967"
     CDS             2571..>2722
                     /locus_tag="PWZ48_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262548.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIR"
BASE COUNT          998 a          396 c          552 g          776 t
ORIGIN      
        1 gtttacatgg tttggatttt tatggggctt gatgaagctg aagctgagta cagagcgaaa
       61 aagccaaccg agggggctga ctaaaaatga atagaaaaga gaaactagaa tgtatattac
      121 tattactcag tttaattctt tcactaattt ctctattggg aagtttgtat ttttgatatc
      181 aacaattttg tggatttggg ttctatttag agtaaatatc atttttgttt ggtgattaag
      241 gagtttatta aaattaattt tccctacttc aaatgcaagg taacaagata tagcactttt
      301 aggaggaata tttagcggta cagcttcaga gtatattcta ttatcatcga ctgatcctcc
      361 tttagcaatt aatttcttaa atggagtagc tgaccatctg cggtttaagt tagcggatga
      421 aagttctaaa ttaactaaca tagctggttc tgttgaatag ttagaaatga ttacttttgt
      481 ataaatcata tctgcagcga agtaagaagc attaagttca acttctagtt gagggcgcat
      541 tccttttatt tttatccctg ttaaaaacgt actcaatata aaacttatta ttgacatcca
      601 ttgaaaaatt gttaagtgaa aaaaattcaa aataaacacc accagttttt aactaaatta
      661 taccaaaaag gagagaagaa ataatgcaag aattagtaat tttgaaaaat aaagaagctg
      721 tgactacgag cttacaagtg gcagaaagct tcgagaaaaa acatcaacat gttttaagag
      781 atattgatgc actaaaaaaa gatgtgtcca attttggaca gatgtttgta gaaggtaatg
      841 aaccagattc atatggcaga aatcgacgag ttttcttcat tagtagagat ggatttttct
      901 tgctggctat ggggtttaca ggaaagaaag ctatctactt caaacaaaaa tacattgaag
      961 cattcaacga aatggaagat gttattcgca agaatactgt tcctcaaaca attgaagata
     1021 tgatgatcta tcaattagaa gaaatgaaag atgttaaaaa agatgtttcc atgcttaaag
     1081 atactatgcg aattagcgga caacaagagt ttgaaattaa gcaaaaagga aatatgaaag
     1141 ttatgggagt tctaggggga aaagaaagcc gagcttatga agaaatcagc aaaaaagtat
     1201 tctcaaaatt ttggtctgaa tttaaacgta ccttttcaat cccaagatat ggcgagttac
     1261 ctcgtaagag attcgatgat gctgtttcat ttattgaaat gtggttgcca gaaactgcga
     1321 tccgtatgga aattgatcaa ctgaacagac aacagagact ttttggtgat gacaatgaat
     1381 agagctgaag cgctaagaat agggacggca attgctaatt gctggtggaa atactataaa
     1441 ccaatcatcc taagccaaca acatattgac aagcaaaaag catggcaaca aataaaaaag
     1501 tgactccgcc ggcaagcaaa gagtcacaaa gaaaacacat cataaggaga ttttagcata
     1561 tggaaaaaga actttccact ctagatcaat atttgattga tcctgattgg ggcaagccga
     1621 aaattgagga aacaagtggt cgaaaaatca gacgaaatct tttgacgaat gaagaactag
     1681 cttgggatca agatgattta ggcaaccatg taactatttg ggatcatgtt tatcttatcc
     1741 atctatcgaa gcattcgaat aaacctgaat atatttacgt catcgaagat ggcttgattg
     1801 atgcgctaga agagtacgac agagataact tgattgatat ctcttattac ggaccaggta
     1861 agaaatacat tgctgaaatg gaggcagaat ttgatgagtg aaatcaaagg gacaacgaac
     1921 tttgaaaaac tttttagtcg taagttaaat aaaattctca agaaaaaagg aaattttgat
     1981 tatttatctt gggctcacgc gtgggagatt atgaaaaaga atgatccaca ggcaacggta
     2041 actattaatg agtacaaaca ctacagggtt gtttctggaa ctcatcaaga ctttcttgtt
     2101 gaggaatata aaccttttct tatggatgaa actgggactt atgtatctgt ctcagtaacg
     2161 gttaaaggac acacggaaac cgaattattt cctgttttag attatcgaaa ccaaccagtt
     2221 gttaaaccaa atgcaatgca aatcaataac tcattgaagc gatgctttgt gaaagcattg
     2281 gctctacacg gactgggatt atatgtattt caaggggaag atattccaac accacctaga
     2341 atcgatacaa agaaattaaa catgctagag acgattctag aagctttcaa tgagcagatg
     2401 ggtaaagata tgaccaaaac cttaatcgaa tatgttaatg agcagacaga taaattaggg
     2461 ctcttagctg ataacgttga aactattgaa cagttaagct atgagcaatg tgccttgatg
     2521 gagcgagcaa tagcagctaa gagaaaagaa ttagataaga agtgatatga gtgtttaaac
     2581 cattaatcga ttcatattca gcggttctga aaaagttcaa aggaaaagac ataagcgcaa
     2641 ccatcaatga ggaagtgaac attgatcgac taaagacgat gtatgacggc tacgatggtg
     2701 atcgagtcat tgaaattcgt tt
//
LOCUS       DANKEF010000152         2642 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000152 DANKEF010000000
VERSION     DANKEF010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.158"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            137..748
                     /locus_tag="PWZ48_002968"
     CDS             137..748
                     /locus_tag="PWZ48_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HDL2262549.1"
                     /translation="MYFILPTLYSLLCVMAPCIIYMVFFCKKNTLKHNILILIFMLYC
                     WKVFELTGTGGLTDIFYRPDTGRKFSLVQGSVNLVPFSGLNISFILNIVMCVPLGFLL
                     PIIWKSYRKIVPVFLAGALFSLIIEVSQIITTRATDIDDLIANTFGAVIGFILWAVLS
                     RVVSLKDKSSTVDYLEPYIYILIGFTGKFLLYFPFWFAFEVLS"
     gene            821..1750
                     /locus_tag="PWZ48_002969"
     CDS             821..1750
                     /locus_tag="PWZ48_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HDL2262550.1"
                     /translation="MKRFLKWGLVLLASLIVILMCTAVVISYVNRPYARAVYDRIKRI
                     LDKDFYDHLYLGSSFEIINLDTMDIHSYMKKDQEELPASLSKLFTIDYALSVLSLDEQ
                     ITISPTALDMQKADSSTAHLQEGDILSVYDIICAMVLPSGNDAAYSLAIYTGGKLDPD
                     AKNDEERYQSFMKNLDRYLSEQGYKHTHFTDAAGYDSNATTSAEDLTKVAAKLLKNDK
                     FAEIVAKETYRAKSIKGNVYNWNTTNQFMQQDNRYFTEGVKGVKTGSFDGRYNLCVYY
                     TSSSDNHYLLVSFGSESDSRRYVDVQNLLHNLP"
     gene            1819..2642
                     /locus_tag="PWZ48_002970"
                     /pseudo
     CDS             1819..2642
                     /locus_tag="PWZ48_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006740602.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
BASE COUNT          925 a          368 c          491 g          858 t
ORIGIN      
        1 cctctaggaa aaaattaact agcaccacgg gttatgatat aataaaatgt attataggca
       61 tttttttaca agaaatagaa gcttgaagtt tatatgaagt tataaaattt aatttatatt
      121 ggaaggagga aaataaatgt attttatact acctacactt tatagccttt tatgtgtaat
      181 ggcaccatgt attatttata tggtattttt ttgcaaaaaa aataccctga aacacaatat
      241 cctgattttg atttttatgc tgtactgttg gaaagttttt gaattgactg gtacaggtgg
      301 actaacagat attttttata gacctgatac aggtaggaaa ttttctctag tacagggcag
      361 tgttaatctt gtaccattca gtggactaaa cattagcttt atattaaata tagtaatgtg
      421 tgtgccctta ggattcttac taccaatcat atggaaaagt tataggaaaa tagttcctgt
      481 tttcttggct ggggctctat tttcactaat tattgaagtc tcacaaatta taactacaag
      541 agcaacagat attgatgatt taatagcaaa tacctttggt gcggttatag gctttatact
      601 gtgggctgtt ttaagtagag tggtgtcctt gaaggacaag tcatctacag tagattattt
      661 agaaccttat atttatatat tgattggttt taccggaaaa ttcttactgt attttccttt
      721 ctggtttgct tttgaagtat tatcctgata attccataga aaataatgag tttgagttga
      781 gaaagaatat tcttgtacac aactctggaa aggaggaagt atgaaaagat tcttaaaatg
      841 ggggcttgtt cttttagcgt ctttgattgt gattctaatg tgtacagcgg ttgttatttc
      901 ttatgtgaat cggccgtatg caagagctgt ttatgatagg attaaacgca ttcttgacaa
      961 agatttttat gaccatctat atctaggttc ttcatttgaa ataataaacc ttgatacaat
     1021 ggatattcat agctatatga aaaaagacca agaagaatta cccgcctcat tgagcaaatt
     1081 atttacgatt gattatgcgc ttagtgtcct aagtttagat gaacagatta caattagtcc
     1141 tactgcactt gacatgcaga aagctgactc atctactgca catcttcaag aaggagatat
     1201 tttatctgtt tatgacatta tctgtgctat ggtcttaccc tcaggaaatg atgcagctta
     1261 tagtttggca atttatacag gtggaaaact tgatcctgat gcaaagaatg atgaagaacg
     1321 ctatcaaagc ttcatgaaaa acttagatcg ttatctaagt gagcaaggct ataaacatac
     1381 tcattttact gacgctgctg gctatgactc caatgctaca acatcagcgg aagatttgac
     1441 aaaagtagct gcaaagctac taaagaatga taaatttgca gaaatagttg ccaaggaaac
     1501 ttatagagca aaatcaatta agggaaatgt ttataactgg aatactacga atcaatttat
     1561 gcaacaagat aatagatact ttactgaagg tgtaaaaggt gtaaaaactg gatcgtttga
     1621 tgggagatac aatctatgtg tgtattatac atctagttct gataaccact atttacttgt
     1681 aagttttggt tcagagagtg atagtaggcg ttatgtagat gttcaaaatc tattgcataa
     1741 tctgccttga aatttcgtgc ctttaagcgc gtatctaaag tttctttgaa aagtttgaga
     1801 atgaagaaag tgagcagtcg aaaaacggtt gctcactttt ttgccctaaa atcgaaaaaa
     1861 tataaaaaag tcctttacaa cacgtctcag gaaaaactgc taagtcaatt ctaatatctt
     1921 gtggagcaag acttgcacct tttgatataa gagagcttag agaactaatg agcgaagatg
     1981 aactagaact cgataaaatt ggagatagaa aaactgcctt gtttgtaata atatcagata
     2041 cagatgatac atttaatttt gtagtttcaa taatgtattc tcaattattt aatctactat
     2101 gtgataaggc agatgatgtg tatggtggaa gacttccagt tcatgttagg tgtctacttg
     2161 atgagtttgc aaatatcggt ttgattccta aatttgaaaa actaatagcg acaattcgtt
     2221 caagagaaat atcggcaagt ataatactgc aagcacaatc tcaattaaaa gcaatttata
     2281 aagaccatgc agatacaata gttggtaact gtgactctac actttttcta ggaggaaaag
     2341 aaaaaacaac agtaaaagaa ttatcagaaa ccttaggaaa agaaaccata gacctatata
     2401 acacatcaga aacaagatca aatcaaaaat cttttggtct aaattatcaa aagactggca
     2461 aggaacttat gagtcaagat gaaataactg taatggatgg tggtaagtgt atttaccagc
     2521 taagaggagc aagacctttt ctatctgata aatttgatat tacaaagcat aagaattaca
     2581 aattgttgga agattatgat aagaaaaact tgtttgatgt agaagagtat ttaaaacaaa
     2641 ga
//
LOCUS       DANKEF010000153         2547 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000153 DANKEF010000000
VERSION     DANKEF010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.159"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..227
                     /locus_tag="PWZ48_002971"
     CDS             <1..227
                     /locus_tag="PWZ48_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262551.1"
                     /translation="KDTKIEEACSDLFGDTNPWFASEEEKKEKQQSVTYERYEILLQQ
                     ATNMQNPTKKETALNYLKSVKEWLDQQQQN"
     gene            complement(348..434)
                     /locus_tag="PWZ48_002972"
     CDS             complement(348..434)
                     /locus_tag="PWZ48_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HDL2262552.1"
                     /translation="MFELFICPLLVGVCVSLFSYWLNNRNKK"
     gene            1079..1816
                     /locus_tag="PWZ48_002973"
     CDS             1079..1816
                     /locus_tag="PWZ48_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015060391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262553.1"
                     /translation="MSNDIVRYNNGLNSVPLRKFTPIDMDLFWGICSKMKRKGTKEEI
                     FTFKQIKELIDYNINARGDRFANDLIKMSKKIITLSFIYEDEKTYEQLNLFQKFKIDK
                     VNETLLIKVSEEFEYILNSIGTNFTRFELENMTNLKSSYVKELYRQLMAHKDRRSKKG
                     HWFVKIEDFRALLAIPESYRMRDIDKQIFAQAKKEFLNVNSTHPAIFKTFEIKKIKAY
                     KGNKIGSLQIYFEENIPSITLDNWLEK"
     gene            1844..2383
                     /locus_tag="PWZ48_002974"
     CDS             1844..2383
                     /locus_tag="PWZ48_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HDL2262554.1"
                     /translation="MQPKETFTIKELSELFEISRQAISKHVDKLDPSLLAKNERGYKV
                     VLRSGVLQLASNLGNQRLLYRLHEYEQENDKNVYISSKNVIVNELLDKLREENEFLRE
                     QLSLKEQNNSNMQKLLDQQQQLTLQANQQIEKLQEQLQLAYTEETPEDESTTILSEET
                     ESIEKHTKKKKQKWWQLWK"
     gene            2399..>2547
                     /locus_tag="PWZ48_002975"
     CDS             2399..>2547
                     /locus_tag="PWZ48_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262555.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEEL"
BASE COUNT          974 a          380 c          459 g          734 t
ORIGIN      
        1 taaaagatac aaagatagaa gaagcctgta gtgatctatt tggagacaca aacccttggt
       61 ttgccagtga agaagaaaaa aaagaaaaac aacaatctgt cacatatgaa cgctatgaaa
      121 tcttacttca acaagcaact aacatgcaaa atccgacaaa aaaagaaacc gccttaaact
      181 atctaaaaag tgtcaaggaa tggttagatc agcaacaaca aaattgaatt tgatttaaaa
      241 tcatgttaaa ctgaatgtgc aaatgatcaa acattagata cacgaaagcc ccagagaggt
      301 cggattctct ggggcttttt ttcaggttga tgtcgccttt attgatttta cttcttgttt
      361 cggttattca accaataact gaataaagag acacaaactc ctacaagaag ggggcaaatg
      421 aacagttcaa acattagata cacctccttc cagtcgcaag actgtcggat aggcgacaag
      481 ttattatatc ttaaaaaatt aaatttgtgt ggtgttttta agtatcactt gttttagggt
      541 tgttagggct tgccctgacc gcctgtaagg cgcttctcga ctagattaag ggaaattaat
      601 caagaattgt cttagaacgg cttagacaag ctttcagagt ctcacacctc ctctttgaac
      661 aacggtttta gcaaaggagc cggaggcgac tgcgcagtat aatatgagag catagcgatc
      721 acaccttttt agcgaccgta gggagcaagt tttccagttt agaaaaattc gcgaaccttt
      781 ttggaggaaa aagtaggagt tttttaggcg ggaaattcaa tgtgctctta agatatactg
      841 ttagatatac tgttagatat actgttaata ataactgtta gggattagca accttatggg
      901 agagtaagta ttgacatagg tagtggggga taaattgtca cactatgggg gataaattgt
      961 cacactatgg gggataaatt gtcacactat gggggataaa ttgtcacact atgggggata
     1021 aattgtgttt tacatcctct tatgtctatg ataacataat ttattgaggt gatttttatt
     1081 gagtaacgat atagttcgat ataataatgg attaaattct gtccctttaa gaaaatttac
     1141 ccctatagat atggatttat tttggggaat atgttctaaa atgaaaagaa aaggtacaaa
     1201 agaagaaatt tttacattta aacaaataaa agaattaatt gattacaata taaatgcacg
     1261 tggcgaccgt tttgctaatg atttaataaa aatgtcaaaa aaaatcatta ctttgtcttt
     1321 tatttatgaa gacgaaaaaa catatgaaca attaaatctg tttcaaaaat ttaaaatcga
     1381 taaagtgaat gaaacattat taataaaggt ttctgaagaa tttgaatata tacttaattc
     1441 aatcggaact aattttactc gtttcgaatt agaaaatatg acaaacttaa agagtagcta
     1501 cgttaaagag ttatatcgcc aacttatggc tcataaagac agaagatcta aaaaaggtca
     1561 ctggtttgta aaaatagaag attttagagc tctattagca ataccagaaa gttatcgcat
     1621 gagagatatt gataaacaaa tttttgccca agcgaaaaaa gaatttttaa acgttaattc
     1681 aactcaccct gctattttta aaacttttga aatcaaaaaa attaaagcct acaaaggaaa
     1741 taagatagga tctctgcaaa tttattttga agaaaatata ccttcaatta ccttagataa
     1801 ttggttagag aaataatacc taacaattta ggaggcaatt atcatgcaac ccaaagaaac
     1861 ctttacaata aaggaattat ctgaattatt tgaaataagt aggcaagcaa tctctaaaca
     1921 tgttgataaa ttagacccgt ccttattagc taaaaatgag cgtggttata aggttgtatt
     1981 acgttctgga gtattgcaac ttgctagcaa cttaggtaac caaaggttac tatataggtt
     2041 gcatgagtat gaacaagaaa acgataaaaa cgtttatata tcaagcaaaa acgtgattgt
     2101 aaacgagttg ctagataagt tgcgtgaaga aaatgaattt ttacgagaac aactttccct
     2161 caaagagcaa aataattcta atatgcaaaa attactcgac cagcaacaac agctcaccct
     2221 acaggcaaat caacagatcg aaaaactgca agaacagtta caattagctt atacggaaga
     2281 aactccagaa gatgaatcaa ctactattct ttctgaagaa acagaatcga tagaaaaaca
     2341 cacaaaaaag aagaagcaaa aatggtggca actttggaaa taactggagg gaacattcat
     2401 gacaagaaaa aatcttctat tcggttctat tttactagga attctaggct tcagtccgat
     2461 gttattgtca gtaatcaata aagggaataa acatggtgaa ttgaaattga atccaaatta
     2521 ttacaacagt cctaacgaag aattgtt
//
LOCUS       DANKEF010000154         2519 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000154 DANKEF010000000
VERSION     DANKEF010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.160"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..555
                     /locus_tag="PWZ48_002976"
     CDS             <1..555
                     /locus_tag="PWZ48_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH32 C-terminal domain-containing protein"
                     /protein_id="HDL2262556.1"
                     /translation="WESPMPEKVDGWAGALTLPRELELIGDRLYMKPVRELEQLRVGS
                     GVETTVNLAGEIIIAQDASFKEALIDVLLTKSKNQEVKFSLKTKDEELINIFYSKDKN
                     ECTLTRNGKNDPRYGTIQMCDRLSLRVFIDKSSAEIFINDGELVFTERYYTENQPSMH
                     LSVSEEAFVSSTIYHLKKDAISYE"
     gene            568..1440
                     /locus_tag="PWZ48_002977"
     CDS             568..1440
                     /locus_tag="PWZ48_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDL2262557.1"
                     /translation="MLIGSIEAGGTKFVCAVGDEDYRVQDQIICPTTTPRETLKKTVD
                     YFKQFDINALGIASFGPIEIRKNSPKSGYITSTPKVGWRNVNVLGYLQDELNVPMSWT
                     TDVNGSAYGEYIMSTLSNEKINSLVYYTVGTGVGGGAVVDGKLLGSFGHSEMGHTFVK
                     RHPDDIDFKGICPYHGDCLEGLVAGPTFEARFGKKGEDVSLTDPIWDVMAYYLAQAVI
                     QTTLIIRPDKIVFGGSVVNEKLLEKIRVNVNKLLDDYVEIPPLSKYITRPLIKNNGSA
                     TLGNFALGLNQLQE"
     gene            1535..>2519
                     /locus_tag="PWZ48_002978"
     CDS             1535..>2519
                     /locus_tag="PWZ48_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2262558.1"
                     /translation="MRPKLEDVAKRANVSKTTVSRVLNNRGYLSQKTIDNVYKAIEEL
                     NYQPNVVARQLFQKKTNIVGLLFPTVANPFFSELVEALEKKLYEIGYKVLIGNSMNNK
                     EKETNYLNQLLSDQVDGLIVGTHNQGIQEYKYQNLPIVAIDRVMNEDIPVVESDNYNG
                     GKLATKLLIAQGAKNIIHTNGPIDLQTPANRRRLAYEDTMKAYQLIPRTVTLDFNISY
                     VKKKQIFFQMFEDYPKIDGIFASNDIDAALILQVAKEKGLNVPADLLVVGYDGTLMTR
                     SILPDLTTVIQPINDIADTAVAILMKRINKEETKKEYILPVTLWMGKTSVKK"
BASE COUNT          906 a          349 c          509 g          755 t
ORIGIN      
        1 tgggaaagcc ctatgcctga aaaagtagac ggatgggcag gagcattaac gcttcctcga
       61 gaattagagt taattggaga tcgtttgtat atgaaacctg tgagagaatt agaacaactt
      121 cgagttggca gcggcgttga aacgacagtt aatttggcag gagaaataat aatcgctcaa
      181 gatgcttcat tcaaagaagc tctaattgat gtactattga ctaaatcaaa aaatcaagaa
      241 gtcaaatttt cattaaaaac aaaagatgaa gagttaatca atatttttta ttctaaggat
      301 aaaaatgaat gtaccttaac tcgtaacggc aaaaatgatc caaggtatgg aaccattcag
      361 atgtgtgata gattatcact tcgagtgttt atcgataaaa gttcggcaga gatttttatt
      421 aatgatggcg agcttgtttt tacagagcgt tattatacag aaaatcaacc aagtatgcat
      481 ttgagtgtta gtgaagaagc ttttgtctcg agtacaattt atcatctaaa aaaagatgca
      541 atttcttacg agtaaggagg ttttatgatg ttaattggta gcatagaagc aggtgggact
      601 aaatttgtat gtgccgtggg agacgaggat taccgagtac aagaccaaat aatttgtcca
      661 acgaccaccc ccagggaaac attgaaaaaa actgttgact attttaaaca gtttgatata
      721 aatgcattag gtattgcatc atttggtcca atagaaattc gaaaaaattc accaaaatca
      781 ggatatatta cgtctacacc aaaggtggga tggcgaaatg ttaatgtgtt aggttattta
      841 caagatgagc tgaatgttcc aatgtcttgg acaacggatg tgaacggatc agcttatgga
      901 gagtacatca tgtccacttt atcgaatgaa aaaataaatt cattagttta ttacaccgtt
      961 ggtactggcg ttggtggtgg agctgttgtt gacggaaaac tattaggatc gtttggtcat
     1021 tcagaaatgg gccatacgtt tgtaaaacgc catcctgatg atattgattt taaaggaatt
     1081 tgtccttatc atggtgattg tttagaaggc ttagttgcgg gaccaacgtt tgaagcacgt
     1141 tttggaaaaa agggtgaaga cgtttcttta acggatccta tttgggatgt aatggcctat
     1201 tatctagcac aagctgtgat tcaaacaaca ttgattatac gaccggataa aatagtattt
     1261 ggtggaagcg tagtaaatga aaaattgtta gaaaaaattc gtgttaatgt taataaatta
     1321 ttggatgatt acgtggaaat accaccgtta agtaaatata ttacgcgtcc tttaattaaa
     1381 aataatggat cggcaacact aggaaacttt gctttgggct tgaatcaatt acaagaataa
     1441 aacatagaaa aaatatcgat tatcaggtat agtagttcta tagagttctt tttttcgata
     1501 gttaattatt gcattattta ggagagacaa gagaatgcgc ccaaaattag aagatgttgc
     1561 caaaagagca aatgtatcaa aaacaactgt ttcacgtgta ttaaacaatc gtggctattt
     1621 aagtcaaaaa acgattgata atgtttataa ggctatagaa gaactcaatt atcaaccgaa
     1681 cgttgtagcg cgtcaattat ttcaaaagaa aactaacatt gtcgggttac tatttcctac
     1741 agtagccaat ccttttttta gtgaactcgt tgaggcattg gaaaaaaagt tatatgaaat
     1801 tggttataag gtattaatag gcaattcgat gaataataaa gaaaaggaaa caaattattt
     1861 aaatcagtta ttgtctgatc aagtagatgg tttaattgtt ggtacacata accaaggaat
     1921 tcaggaatat aaatatcaga atttaccgat tgttgcaatt gatcgagtga tgaatgaaga
     1981 tattccagtt gttgaatccg ataactacaa tggtggaaaa cttgcaacca agttattaat
     2041 tgcacaaggt gcaaaaaata ttattcatac aaacggtcca attgacttac agactccggc
     2101 taatcgtcgg agactagcct atgaagatac tatgaaagca tatcagttaa ttccacgaac
     2161 tgtgacatta gatttcaata tttcttatgt aaagaaaaaa cagatttttt tccaaatgtt
     2221 tgaggattat cctaaaatag atggtatttt tgcttcaaat gacatcgatg cggccctaat
     2281 attacaagta gcaaaagaaa aggggttaaa tgtaccagct gatttattag tagtcggata
     2341 tgatggtact ttaatgactc ggagtatatt accagatctg acaacagtaa tacaaccaat
     2401 taatgatata gcagacacag ctgttgctat tttgatgaaa cgaataaata aagaagaaac
     2461 aaaaaaggaa tatattttac ctgtcacatt atggatgggg aagacatcag taaagaaaa
//
LOCUS       DANKEF010000155         2517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000155 DANKEF010000000
VERSION     DANKEF010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.161"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            5..2377
                     /locus_tag="PWZ48_002979"
     CDS             5..2377
                     /locus_tag="PWZ48_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262559.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEEYVEKLERQEELKEMGYNKYGGLDM
                     TIDNIPRIKEEIERFEKGESAFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     RPTDSMKEKENDHFYRVEFNENDPEKGLKSYGGEIVTFELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         1006 a          405 c          555 g          551 t
ORIGIN      
        1 gaacatggca gaagaaaaag aaccactaga caatagtcgt ttgggaaaat ccaaaagaaa
       61 actagtacga ttgcaaaatg aattaaacga acaaattgaa aagatgtttg aacaccaaaa
      121 aatgacgaac ggtcaaccaa tgaacgacaa gaggaatggc cgttcatggt ttaaccagca
      181 agaacgaatc gaaaataaag tccaaagtct gagagaagag atcaagcaac aagaagaata
      241 cgtggaaaag cttgaaagac aagaagagct aaaggaaatg ggctataaca agtacggcgg
      301 actggatatg acgatcgata atattccaag aatcaaagaa gaaatcgaac gctttgaaaa
      361 aggtgaatcg gctttttcag ccgccacgat ccgaaaatac caacggaagt tggaaacatt
      421 agaacagctg aaagaaaaat cagaaaaagg aaaagaaaat atcctccctg aagtacaagc
      481 gatcattgac agcggacgag tgacgcaatg gaaaaaaaat ccaaccattt attttttgaa
      541 aggctatcga aaagttgctt tagaactgaa tgaaaacggg ggatttgagg aatcaaaaaa
      601 ataccaagcc acgtcggaag aggaaaaagc agctgttcgt gaattacttg aagaggctag
      661 accaactgat tctatgaagg agaaagaaaa cgatcatttc tatcgagtag aattcaacga
      721 aaatgatcca gaaaaaggat tgaaaagtta tggtggggag atcgtaacat tcgaattgat
      781 cgatcaactc aaaacattgg ataaccaata cgccgcagaa gcaggttatt ataaattcta
      841 ttttgaggaa gtgaagaacg gagaagtagt cgatcagatc cgaatggatc taggggatgg
      901 cgtcaacagt aatcaaccaa tctatttaga gttagcaaaa ttgtgcgaac aagtacctga
      961 gaaggaacaa aaacaatcgg tcaaaaatga acctgtttcg aaagaacaaa tgcagtcgaa
     1021 agaaacaaaa acaaaacaaa agggctatca aaaacgaacg gcagaagaga ttaaacaaga
     1081 agttaaatcc ctttcgcaac aggcactgga tgcggtgaaa aagcataccc attctcctga
     1141 agacgtcaaa gaacttttga actttatgtc tcgttttcca gaacgatcct ttagaaatca
     1201 attattgatt gaggaacaat ttccaggtgc aacggcttgt ttaggtcgtg ccgcgttgaa
     1261 aaaagaagga atctatatca aaaaagggga acaagggaac aagatctttg tcagaaaaac
     1321 ggtcaaaggg ttttatgaga aaggacgtgg ctttgttcga gaaacagaag ctacaccaga
     1381 agataaaaaa cgaatcgaaa gtggtcaaat cgaagtgatc aaaaaacctt actatacgat
     1441 tgaaaaagtg tttgatatca cacaaacaca gttaaaacct gaagactatc cgaaaatctt
     1501 tccgaaccgt gtctttgatt ttcaattgga taaaaaagga cagtcagaac ttcaagctgg
     1561 gattcaagcg gtagccaaca atattggaat acaaattcga gatatgacag aaagtgaagt
     1621 ctatcaaaga gaattaggac aagcacgtgg ggcgtatgtt cgtaacgaat ttactggcga
     1681 agaagaaatc gtcatgaata ctcgaaatac tcctacgcag catttagcta caagtattca
     1741 tgaattagcc catgcaagaa tgcacaaatt ttcagaatta gataccgcaa ctaaagaatt
     1801 gcaggcagaa atgacttctt atattgtgtg caaacatttt ggaatggaca cgtcggagaa
     1861 agctattcct tatattgcag catggacgaa gaatggtcag acgttggatg ataaggaagc
     1921 tgcggaacgt gggaaaatca tgaatgatgt cagccgtgtc gcaaatgaat tcattcaaac
     1981 catttctaat gaaatcaatc aatacagaga aagagaacca gagatccaac cagcagaagt
     2041 gaagcaggag cataaaaata ctttagaaac gccagagaat aagaatattg tgcccgttgg
     2101 ttcactttgg atcgacaaga aagacgggaa agtaatggaa gaagatacgg gtcgaacgtt
     2161 acatttgaag gatgcggcgt ttattgaaga tccgcagcat aatcaagtaa ttttaaaagg
     2221 aaaaagttat ggaaaagaga tccaaacggc gttaccttca gaagaatttg cgctaggaaa
     2281 tccattccga ttttcacttg agacagtgtc agaagtggca aaacgaaaaa atgaaatcta
     2341 ttatgaacaa caattgtcac aaaaagaacg tgtataaaac aagtaaaaaa gactatctga
     2401 aaaacggata gtcttttttc ttttgggagg ttttaaaatg accgagaaaa aaatgagtct
     2461 tattgatcga tgtaaacaga tcgatatagt ggattttgct cgaaacaatg gaatggc
//
LOCUS       DANKEF010000156         2429 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000156 DANKEF010000000
VERSION     DANKEF010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.162"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..521
                     /locus_tag="PWZ48_002980"
     CDS             <1..521
                     /locus_tag="PWZ48_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDL2262560.1"
                     /translation="SNANAEAALTIAIPISVLGQILGILMRTILSNLTHVADRFIEQG
                     KFSQARRIHILYGSGLYALMYFIPIFLAIYFGTNLVSQIVDAIPEWLTNGLTVASKIL
                     PAYGFALLLQTMLTKKMVPFLLLGFLITAYSGLGIIGIAAFACIVAFVMVQVQGKNSN
                     GNQNEPDPLDDL"
     gene            535..1347
                     /locus_tag="PWZ48_002981"
     CDS             535..1347
                     /locus_tag="PWZ48_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDL2262561.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            1401..2366
                     /locus_tag="PWZ48_002982"
     CDS             1401..2366
                     /locus_tag="PWZ48_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2262562.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
BASE COUNT          800 a          376 c          501 g          752 t
ORIGIN      
        1 tatcaaatgc gaatgcagaa gcagcattaa cgattgccat tccaatttca gtgttaggtc
       61 aaatactagg tatcttaatg cggacgattt tgtcaaattt gactcacgtt gcggatcgct
      121 ttatcgaaca aggaaaattt agccaagcaa ggagaattca tatactttat ggctcgggat
      181 tatatgctct catgtatttt attcctattt ttctagcaat ttattttgga acgaatcttg
      241 tttctcagat tgttgatgcg attcctgagt ggctaacaaa tggcttaacg gttgcaagta
      301 aaattttacc agcatatggg tttgcgttgt tattacaaac gatgttaacg aaaaaaatgg
      361 tgccattttt attacttgga tttttaatta ctgcttattc tggattggga atcattggta
      421 ttgcagcctt cgcatgtatt gttgcgtttg tcatggttca agttcaaggt aaaaactcaa
      481 atggtaacca aaatgagcca gatccattag atgacttata aggaggtaga ttaagtgaaa
      541 gagaaagatc aaaatgcatg gaaatatcgg cagttcttat ggcgttcatg ggcaattcaa
      601 gcctcatgga actatgaacg acaaatgaac atgggtttta tgtacggaat tgcaccaatt
      661 ctagataaca tttacaagaa tccagaagat gaagcaatga agaaagaagc ttacgagcgg
      721 catatgatgt actacaactg tacccctcaa acaagtgcat ttgtgttagg gttaactgct
      781 tctatggagg agcaatacta cgaagaccaa gaaaacttta atccggaatc aatcagtgca
      841 gtgaagactt ctctgatggg accgctatct ggtgttggtg attctttctt tcaaggaacg
      901 attcgggtat tagcctttgg actaggaatt aatttagccc aacaaggtag tattttaggt
      961 ccaattctag caatgttaat ttcctttgta ccttcctttt tagttactta ctatggtggg
     1021 aaaattggct ataacatggg gaataaatac ttgtctaaac tgtatcaaga aggtttgatg
     1081 gataaagtaa tgtatgtttg ctccattgtg gggttgatgg tcattggttc gatgattgcc
     1141 agcatgatcg ggattacaac accaattcaa atgggtaaag cttttgtctt acaagatgta
     1201 ttagatagta ttatgccaca atttttacca ttaggattaa ccttcttcat gtactggtta
     1261 ttgcagaaaa aggtcaaaac aggatggatg ttggcaatct gtttagtcgg aggaattatt
     1321 tttagtttgt taggtatctt acaataaggg aataaccaat aaatttggtt gaataatata
     1381 aataaggagg aattcaattg atgaattcaa aagaaattgt taaagaaatc gtagcgaaaa
     1441 aagaggtaaa atcaatcgta tttgttggct gtggcgcttc aaaagcggac ctttatcctg
     1501 caaagtattt tctagaccaa aatgcgaagc agttacggat tagtcattac acagccagcg
     1561 aatttaacta tgcaacacca gcatctgtag atgagtcaac tgtcgttatt tcagcttctt
     1621 taggaggtac gactccagaa acagttgaag ctaacgcagt agctaaagaa aaaggtgcta
     1681 cagttattag tctaacacga agcattgact caccgttaac aaaagatgca gattatgtaa
     1741 tttatcatcg atttgcagag aactatgctg ctaaattaga aaaaacaggc tactgtttag
     1801 aattggcagt agaactttta catcaagtag aaggttatga ccattatgaa gcaatgcaag
     1861 aaggatttgc tactatctat gatcttgccc aaaaaagtgc agaatcagct cgtaaagatg
     1921 cgaagaaatt tgctgaacgc tttaaagatg aaaaagttat ttatgtaatg agtagtggtg
     1981 ctacacatga agtagcatac tcaacatcaa tttgcttgat gatggaaatg caatggatta
     2041 attctggtac cttccattca ggagaatttt tccacggacc attcgaaatt gtggataaag
     2101 acgtaccgtt tatattatta atgaatgatg gaaaaactag aaatatagat gctcgagcat
     2161 taactttcct tgaacgcttt gatacgaaaa cagaagttgt agatgccttg gattatggct
     2221 tgtcttcaca tattgctaaa gaagtcttag actactttaa cccattcgtg attactgcag
     2281 tatttagagt ttatgcagaa gaattgtcag aagcaagaga acatccatta actaagagac
     2341 gttatatgtg gaaactaact tattaaaaaa tagatcaaac taaataaggt ggcttgagaa
     2401 attaagccac cttgttttct tcaatgtga
//
LOCUS       DANKEF010000157         2418 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000157 DANKEF010000000
VERSION     DANKEF010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.165"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            308..928
                     /locus_tag="PWZ48_002983"
     CDS             308..928
                     /locus_tag="PWZ48_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDL2262563.1"
                     /translation="MILSVENFTLSINKKKIKQNWNFSISEKDFIIVTGKSGSGKTTL
                     LNNLSLIDKQYTGNLFYFEKKVSKHDIQRLRRNHISYLFQNYALLENQTVQYNFELAK
                     KFNSNISNDHIYNLLTDFSLPKDILKEKIFLLSGGEQQRIALIRNMLKPFDILFADEP
                     TGNLDKENSTFIMNFLKNLTEKEKKAVVLVTHDLNVLNYGTNIIEL"
     gene            1042..1179
                     /locus_tag="PWZ48_002984"
     CDS             1042..1179
                     /locus_tag="PWZ48_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262564.1"
                     /translation="MILLKKEYEKFYDRLIYSLQLPKKLNRQLQKTYETKSTEEWESY
                     C"
     gene            1621..2001
                     /locus_tag="PWZ48_002985"
     CDS             1621..2001
                     /locus_tag="PWZ48_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HDL2262565.1"
                     /translation="MSEQNQNLASNSSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVASG
                     DAQRLSENLKIMQKELQKIWQQLR"
     gene            1983..>2418
                     /locus_tag="PWZ48_002986"
     CDS             1983..>2418
                     /locus_tag="PWZ48_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDL2262566.1"
                     /translation="MATIKVSRTTSCSRAINYAEPRATIKTGINCDVDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQTAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLAHNLPITE"
BASE COUNT          908 a          381 c          387 g          742 t
ORIGIN      
        1 tacaatatct tttaatatta ggattattta taataattaa ttttcaaatg atttttgtta
       61 gttttactat aaattcaaaa aaaatagctt taaaaaaaat atttggatat tcaccaataa
      121 gtcaagtttt agatttatgt ttactccctt ttacaataga gttatttgct actatgtgtt
      181 ttcaattgat attttcaaaa aacacttact tgtattttat aatttttatg atctttttaa
      241 taaatctaat tacttctatt attatttata aaaaaaatag ttttaaatat tttaaggaga
      301 atattttatg atattatcag ttgagaattt caccctgtca attaataaaa aaaagatcaa
      361 acaaaattgg aacttttcaa tatcagaaaa agattttatt atagtaacag gaaaaagtgg
      421 tagtggaaaa actactctgt taaataattt aagcctaata gataaacaat ataccggtaa
      481 cttattctat tttgaaaaga aagtttctaa acatgatata caacgtctac gaagaaatca
      541 tattagctat ttatttcaaa actatgctct tcttgaaaat caaacagtac aatacaattt
      601 cgaattagca aaaaagttta acagtaatat ttccaatgat catatttata atttattaac
      661 agatttttct ttgccaaaag atatcttaaa agaaaaaatc tttctcttaa gcggtggaga
      721 acaacaaaga atagcattaa ttagaaatat gttaaaacca tttgatatat tatttgcaga
      781 tgaacctaca ggaaatttag ataaagaaaa ctcaactttt ataatgaact ttctgaaaaa
      841 tttaacagaa aaagaaaaaa aagccgttgt actagtaaca catgacttaa atgttctaaa
      901 ctatggaact aatattatag aattataatt tttacccgtg atgctagctg atttaaaact
      961 agcaccacgg gtatttattt tatattccat ctctcctatc ccaaaattta aataatcact
     1021 taataaaaag gaggcaccct agtgatttta ttgaaaaaag agtatgaaaa attttatgat
     1081 agacttattt attctttgca attgccaaaa aaattaaacc gtcaattaca aaagacttat
     1141 gaaactaaat caactgaaga atgggagagt tattgctaga tttttggtag aagtaacaaa
     1201 aagtggacat attatcataa tgactcgccg aaagtctgat aaaaaattgg ctaaaaatat
     1261 tgctgaatgt ttaataaaac tatcagtttt agctaaatag ttagtagttt acacaaaaaa
     1321 gcatttatgt tttttgttgc aaagtaacaa tttcatcttc ggtgaaataa gcgaaacttt
     1381 acaaaaagtg gctagcattt gctagctttt tttgtattgt ggagcgtggc ttttgagggg
     1441 gattcaaggg ggagcttgcg cccctttgac aagcggttat ttgcactaaa tacgtaggta
     1501 tttggctagc aaatatagag cttgccaaac ccttttagtg agctttactc attgtacctt
     1561 tttaggtaca atggtattgc gaacgatacg tccgtaggac gtactgaaag ggtgggactt
     1621 atgagcgaac aaaatcaaaa tttggctagc aattcttcta aaaaatatac ctatcgatcc
     1681 gagccaaaac aaatcagttt tcgagtgagc gaatccgaat ttgcaaagct gaagcaatcg
     1741 gctgaagctt tgcaaatgag tgtgccggct ttcgtgaaag ccaaggcaca aaacgcgcgt
     1801 ctggtgacac cgaaagtggc gccagacatt gcccaggcaa tcgcgcgcga tttagccaaa
     1861 gctggaggga atatcaatca aattgctaag tggtgcaata cacaccaaca tgacgttgct
     1921 tctggtgacg ctcaacgttt atccgaaaac ctaaaaatca tgcaaaagga gctacaaaag
     1981 atatggcaac aattaaggta agccgaacca cttcttgtag tcgtgcaatt aattacgcag
     2041 aaccacgagc aaccattaaa acaggaataa actgtgacgt cgattatgcc aaaagtgaaa
     2101 tgaaacaaat tcgcatgctg tatggaaaag acgaccgtgt acaggcacac ttattgattc
     2161 aatcttttcg accaggagaa attaccgctg aaaaagctaa tcaactaggg gcagagtatg
     2221 caaaaaaaat agcccccgat caccaaacag ctatttacac acatacagac aaagaccata
     2281 ttcataatca cattgtcatt aactccgtta atctggagac tggaaaaaaa tttcaagctc
     2341 atggtcaagc atttctaaac aaatgttatg acctaaatga cgagatttgt cttgcacata
     2401 acttaccaat cactgagc
//
LOCUS       DANKEF010000158         2316 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000158 DANKEF010000000
VERSION     DANKEF010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2316)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2316)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2316
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.167"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            288..542
                     /locus_tag="PWZ48_002987"
                     /pseudo
     CDS             288..542
                     /locus_tag="PWZ48_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            612..1124
                     /locus_tag="PWZ48_002988"
     CDS             612..1124
                     /locus_tag="PWZ48_002988"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262567.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1121..2158
                     /locus_tag="PWZ48_002989"
     CDS             1121..2158
                     /locus_tag="PWZ48_002989"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2262568.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT          842 a          419 c          465 g          590 t
ORIGIN      
        1 ataaattatc taaagagaat taagttttga ttatgtaata agttttggta ctaaaaaaga
       61 cttgatctaa ttagaccaag tcttttgtta gtgttatatt aataacaaaa taaaaataag
      121 aagtcgctca ctccccgacc aaagtttgtg agcaaaataa acagtcgatg tttgtttctt
      181 aattgtatat cttgatatta aacgatttaa gattattctt caagatatat attcgcaaat
      241 gtaattggaa aagcgacttc ttaaacaaaa ttaaggagtc gctttttatg gcaaaaataa
      301 cgaaaaatga gcaagaaaat ttggtggaaa attacacaaa aaaaccggcg tactctaata
      361 gccggttaga cgcacatttc gtgtgcactt ctaatccgaa attgagcttt gacgctatga
      421 cgatagtcgg aaaccttaat aaaaacagcg ctaaaaagct atccgatttt atgagtttag
      481 atccacaaat tagactttgg gatatacttc aaacaaaatt taaagctaaa gcactacaag
      541 accccctatg ttaggctagt tgtcgtatag gataataagt tataataaag actatacgga
      601 ggatgattac catgacaaat aaaaacaatg aacgttactc taccgaactg aaagcgacag
      661 ccatcaagaa aatgatgcca cccgaaaata gggggattcg ccaattaagt caaactttgg
      721 gcattccaga agcgactctt tattcttggc gtaaaaaagc tagaattcaa gggaatacta
      781 cacccggaac gaaaaaaaat gtcgaaaaat ggtcaagtga agataaattt ttaattgtga
      841 tggaatcatt ctcaatgaac caagtagaac ttgcggaata ttgccgaaag aagggcttat
      901 ttaaagaaca aattgaggct tggaagaaaa cgtgcttatc tgcgaatgaa caggaagaaa
      961 atcgaacgag agaacttgca actgaactaa aagaagagaa aaaacaagcg cgtcaattag
     1021 agaaagaatt acgtcataag gaaaaagcat tagcggaagc cgctgctctc ttattactaa
     1081 gaaaaaaagc acacgcgatt tggggggacc aagaggaaga atgatttcat cttcagatcg
     1141 caaacaagct gttgaactta ttcaagaagc caatcgcaat ggggcccgtc tcacctatgc
     1201 ctgtaatgag ctgaatatta gcgttcgaac ctacgaacga tggacgcgtg aaggtacgat
     1261 agcgcatgat caacgccctt tggcgaaacg tcctgtaccc aaaaataaac tgacggatca
     1321 agaacgcgaa aaaataatag aaaccgtttc aaaaaaagaa tttatgaatt tgccacctag
     1381 ccaaattgta ccaaaacttg cggattgctc gatttatatt gcatcagagt ctagcttcta
     1441 tcgtgtattg cgagaaaaaa atatgcaaca tcaccgtggc cgaagtcaag tttcacaaaa
     1501 acgcataccg ccaagtcacc ttgcgacgaa gccaaatgaa gtttggactt gggatattac
     1561 ttggctaaaa ggtccaataa aaggactctt ttaccggtta tatctcatca ttgatttatt
     1621 tagccgtaaa atggtaggct gggagatttg ggaatcagaa gaagctcgtc atgccgaaaa
     1681 acttattaaa cagacggtgc ttaaggaacg tatccaagga cagccgttgg ttttacactc
     1741 cgataatggc agccctatga aagccgcgac ttttcaagtc ttactcgaaa aattaggaat
     1801 tcaaagttct tattcaagac cacgagtgag taatgacaat ccttattcgg aagcggcttt
     1861 ccgaacgtta aaatatcgac cagaatatcc caataacggc tttaaaacaa tagatgatgc
     1921 tcgaaaatgg acgaaagagt ttgtcgattg gtataacgat tctcatttgc atagtgggat
     1981 tcaatttgtg tctcctgcag cgtgtcatga aggacgacat ctcgctattt tagagaaacg
     2041 aaagcaggtt tacgaggacg ctaaaaagcg ccatcctgaa cgctggtcaa aaggtgttcg
     2101 aaattggact ccacatcaat cggtggcgtt gaatccttta aaagaaacag aaagttaaca
     2161 ggagcataat ctttcaagat tatcaaataa ccctcgtcac ccaatagggg aaaaagttat
     2221 tcttccccta ttggataacg aggccaaccg accataggga ggttctgctt tttgataggt
     2281 ggaaaataaa aatacgacaa ctatattgac aaacac
//
LOCUS       DANKEF010000159         2253 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000159 DANKEF010000000
VERSION     DANKEF010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2253
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.169"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            581..1540
                     /locus_tag="PWZ48_002990"
     CDS             581..1540
                     /locus_tag="PWZ48_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2262569.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVLPDGQQEYIQKKVAQGWTPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNK
                     VSETFIQSVASKRNHIPRKSLHYRTPLEVFLSYVGKDDLSSLI"
     gene            complement(1527..2132)
                     /locus_tag="PWZ48_002991"
     CDS             complement(1527..2132)
                     /locus_tag="PWZ48_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262570.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYKVSFVKLS"
BASE COUNT          768 a          363 c          334 g          788 t
ORIGIN      
        1 aacaataaat gaagactttt ttattgttaa ttgtttattt ttctattatt agatatgaaa
       61 atcgcattta gataaaatag aaaaatatga atatatttta aatattaaga tgagagtcat
      121 taacttatga tagtagtcaa tttgcttttc actaattaca aaatatgtga tataggaagt
      181 ttagttattg ttctcataat gccatcctgt ttttagatta tctagtaatt tttttgttat
      241 ttaaaattag gagcagtgcc aattgtttta attttaaagg ctttttttat atcttctaaa
      301 ttagaagata tttgtatatc aatatggaat atattacctt ttttgtattt ttttatgtta
      361 tctttagcta aaacatcaaa tacatgtcct tgaaattgta tcgaggcagc tggatttagt
      421 ttaaattgag gaaactttac cacagctgat tcaaaacact tgtcacattt ttttattttt
      481 ttaccttcgc cgattgtaaa attaagctag acaactaaaa aagtcatttg tgctacactc
      541 aaaatagttc cgtcgaaaga attataagga gtgagtacaa atgacctata cccatcttac
      601 accaactgaa ctcgtaatga tagaagctta tttcaatcaa tcacactctg tttcaaaagt
      661 agcccatctg gtacaacgtt ctcgacaaac catttataat gtctatcgtt ttcttaaatc
      721 tggtggaagc gcaataagct actatgaaca atataagaaa aacaaacgta actgcggaag
      781 acgtccgatt gtgttaccag atgggcaaca agaatacatt caaaaaaagg tggctcaagg
      841 gtggactcca gatgtcctta ttggtcgagc agagtttcct attgcttgtt cggtacgtac
      901 actatatcgg atgtttaaag ataaaacctt tgaccctcac gatttaccaa tgaaagggaa
      961 aagaaaacca aacggacata aagaaaaaag aggaaaacaa gcttttcgcc gttcgattca
     1021 ggatagaaca actgattata tacagttcaa tcatgagttt ggtcacctag aaggtgacac
     1081 cattgtcggt ggaaaacata aaagtgcggt gatcacgtta gttgagcgat tatctaaagt
     1141 gatcatcacc ttgaaacccg ctggtaggca agctatagat attgagaata gactgaatca
     1201 atggtttcaa tctgtaccga aacacttgtt taaatccatc acatttgatt gtggaaaaga
     1261 gttctcgaat tggaaaaaga ttagtaatac caatgatatt tcgatttatt tcgctgatcc
     1321 aggaacacct tctcaacgag gactaaatga acattcgaat ggcttattac gtaaagatgg
     1381 cttatataaa gctatggatt ttaataaggt atccgaaacc tttatccaat cggtggcatc
     1441 aaaacgaaat catattccta gaaaatcatt acactatcgc acaccattgg aagtattttt
     1501 gagttacgta ggtaaagatg atttgtctag cttaatttga caaacgaaac tttgtaaaaa
     1561 tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat gtcaatattt
     1621 ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa ttccctgtaa
     1681 ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc acccattcca
     1741 ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc agtttgttta
     1801 tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc aaaaattttt
     1861 tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac tgataattga
     1921 tactctatat tacatgactt aattagtttt ttagatcccc cctccagata ggcatatgtt
     1981 tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc ataaattaaa
     2041 aatgactgtc tatttagccc ataatctaat acaacttctt catactcata tttctttaaa
     2101 tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt gttatttatt
     2161 gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt agtgaactac
     2221 tcaccactta gctaaaccta acggtttctt acg
//
LOCUS       DANKEF010000160         2221 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000160 DANKEF010000000
VERSION     DANKEF010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2221
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.170"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..159
                     /locus_tag="PWZ48_002992"
     CDS             <1..159
                     /locus_tag="PWZ48_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2262571.1"
                     /translation="RRNKFYRSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGIPA"
     gene            242..859
                     /locus_tag="PWZ48_002993"
     CDS             242..859
                     /locus_tag="PWZ48_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011834674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262572.1"
                     /translation="MWIFKQEIFAKLFSLDGLGWGILFFIFLFLGSMWIWFVDKKNSY
                     EKVIVKKMNRNEFDDFSKEHRVELYDSHEINSTSPSYYWVFGCEEKPLEIKLLKMLEN
                     PPVIEDFSAKNVEKQMFEQVQIRGQNKISLEPDEYAAFFLETNSGTSMPVFKVKLKMN
                     GRLCTYDLVANMRYGSTGDRRTILKFEQTFRSFLKNRVSQIFNLD"
     gene            1023..1355
                     /locus_tag="PWZ48_002994"
     CDS             1023..1355
                     /locus_tag="PWZ48_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262573.1"
                     /translation="MNNREILAKEMADLKAKEAKIRAKRKQIRQKEMISLAKLYCKKE
                     GFKTPAEALDFLESNKRNHSVNDEVLVSLRKFADGLVWQENGAYWRTNDVKGLTQFMA
                     QFRSENVN"
     gene            1679..1840
                     /locus_tag="PWZ48_002995"
     CDS             1679..1840
                     /locus_tag="PWZ48_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HDL2262574.1"
                     /translation="MAYMVARTQKHKVGSLNGLQNHVDRKTKNHTNKDIDITKSGSVA
                     KFSKKSILV"
     gene            1872..2221
                     /locus_tag="PWZ48_002996"
                     /pseudo
     CDS             1872..2221
                     /locus_tag="PWZ48_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
BASE COUNT          784 a          291 c          451 g          695 t
ORIGIN      
        1 cgacggaata aattttatcg aagtctccgg actgcctcat ccacgattaa ggggatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaga tcaaggtatt aatgggaata ccagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga accattattt taaagtggga ggaaaattgt
      241 tttgtggata tttaaacaag aaatctttgc taaattattt agtttagatg gattaggttg
      301 gggaatctta ttttttattt ttttattttt aggatctatg tggatctggt ttgttgataa
      361 aaaaaattct tatgagaaag taatagtgaa aaaaatgaat cgaaatgaat tcgatgattt
      421 ttcaaaggaa catagagttg aattgtacga tagtcatgaa attaacagta cttctccatc
      481 ttattattgg gtatttggtt gcgaagaaaa accattggag attaaattgt tgaaaatgct
      541 tgaaaatcct ccagtcattg aagatttttc tgcaaaaaac gttgaaaaac agatgtttga
      601 acaggtacaa ataagaggtc aaaacaaaat ttcattagaa ccagatgaat atgcagcctt
      661 ttttttagaa acaaattcag gtacttcaat gcctgttttc aaagttaaat taaaaatgaa
      721 tggtcgttta tgcacttatg atcttgttgc aaatatgcgc tatggttcta ctggtgatag
      781 acgtactatt ttaaaatttg agcaaacctt tagaagtttt ttgaaaaata gagtatccca
      841 aatttttaat ttggattgaa aaaattaaaa atttggccaa aaatactaca gttttttaga
      901 gcaatcatct tatgatgatt gctctttttt tgttgttttt gtgtcattcg ttgttcaaat
      961 cctctattcg taaaataaat attttaaact agaaatatgc cttattttta ggaggaatca
     1021 aaatgaacaa tagagaaata ttggcaaaag aaatggcgga tttgaaagca aaagaagcga
     1081 agattagagc gaaacgaaaa cagattagac agaaagaaat gatttcttta gcgaaattat
     1141 attgtaaaaa ggaaggcttt aagacacctg ctgaagcatt agattttttg gaatcaaata
     1201 aaagaaatca ttcagtaaat gatgaggttt tagtatcgct aagaaaattt gcagatggat
     1261 tagtttggca agaaaatggt gcatattggc gtacgaatga tgttaagggt ttgactcaat
     1321 ttatggcaca atttcgttca gaaaatgtga actaaattga ggaggggttg aagtgaaaag
     1381 caatactttt cacttttggt tttttgcgga ataaaaattg tgaaaacaat gacgcaaaaa
     1441 gccaatggtt ttgaacgaaa aaaggacgta aaaaaggaat gcgaaggcag tcgtttttcg
     1501 ttcgcttttt cgtttgagag aaacggagtt tctcatcggc tgttaagccg atatagccct
     1561 cggcagagct tacagacttt acgcagtata agtctagtaa gttatacctt acatggaggt
     1621 ttttccgaat ttagcttgtg attctcgcaa taattttaac gaaaggaagt acacagttat
     1681 ggcctatatg gtggcacgaa cacaaaagca taaagttggg tcgttaaatg gacttcaaaa
     1741 tcatgtagat cgtaaaacga aaaatcatac gaataaagac attgatatta cgaaaagtgg
     1801 ttctgttgca aagttttcca aaaaatctat tttagtgtaa aattgagaaa aaagacagag
     1861 aggatagagt aatgaatcat tttaaaggca aacaattcaa aaaatgacgt cattattgtc
     1921 gctgttagtt actacctgcg ttacaatcta agctatcgtg aagttcagga attgttatat
     1981 gatcgtggaa taaatgtttg tcatactacg atttatcgtt gggtgcaaga gtacagcaaa
     2041 gtcctcgatt atctttggaa gaagaaaaat agacaatcct tctattcatg gaaaatggac
     2101 gaaacctata tcaaaattaa gggacattgg cattatcttt atcgtgcaat tgatgcggac
     2161 ggcttaacct tagatatctg gttacgaaag aaacgggata cgcaagcagc ctatgctttc
     2221 t
//
LOCUS       DANKEF010000161         2189 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000161 DANKEF010000000
VERSION     DANKEF010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.171"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(325..528)
                     /locus_tag="PWZ48_002997"
     CDS             complement(325..528)
                     /locus_tag="PWZ48_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262575.1"
                     /translation="MSQLGKCYSLSKSNRKVYTLFISDSAKEGFTLGFSISFKKGTKN
                     TSISHNNRELTDSQKINGLSGRK"
     gene            complement(987..1952)
                     /locus_tag="PWZ48_002998"
     CDS             complement(987..1952)
                     /locus_tag="PWZ48_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2262576.1"
                     /translation="MSKRGTIINEWENLTALSNRRLSVQTLTTPLKIQWSIDRITIVG
                     KLKENIYYHTQNDVLILDFEQLMRLNENNGYVKSVGLNGWQIVDKHDENIAYIEILKY
                     QEGQGRIDFNPNKISQFLAGSMKNFIHDLFLEPHFSRADIACDIIDVPDDFITQYRVV
                     DPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQETKRKIVPPEIKTWWRLELQ
                     LRRGKATDWYAMVHESLDSFASPHFLPTDTKHTDRIMIAGLISDQNLWAGLARHTKYK
                     YRNLLKKESQSDELVNHLRETFSESADVLKKELDTWLLGLDVTEE"
BASE COUNT          629 a          506 c          346 g          708 t
ORIGIN      
        1 aaaaaaataa aaagacctca gggaatttca ttccccgaac cccttgagcc tcctcctgcc
       61 gaaatggaaa gggcgcaccc gctgtttatt tcattcagct atcggctttc acaatctaac
      121 acgatcgttc cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttta
      181 acgattgtgc cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc
      241 cattttagag ctattgtaga aaatgtagtt tgaaatgcaa ttttcttctt tcagcttttt
      301 atttcccact tgtaaatctg ctaattattt tctaccagac aaaccattga ttttttggct
      361 atcagttaac tcacgattat tatgagaaat gcttgtattc tttgttccct ttttgaacga
      421 aatactaaac cctaaggtga acccctcctt cgcgctgtcg ctaataaaaa gagtgtaaac
      481 ctttctgtta ctctttgaca aagagtaaca ttttcctaat tgtgacatgg ctgtcacaat
      541 tccaaacgct ctccgagggt tttcgctgtc acaagtgaca acgaaaaaag gagccgaaag
      601 cgtgtcacgc tctcaactcc ttttacgctt tattttgctt cacgcaaaat aatcgcttat
      661 cttaaacatt gacgtaaatt tactctcact tatctgactt ttttactcgg tatcactccg
      721 agtaagccgt catattcatt gggataatct cgtctgcttc gctctaattt tccagagtga
      781 ggggtaactc cttcccccga attaccccaa agagtgacac tgggatatcc ctaaatttat
      841 tataaaaagc caagtctctt tgcgcaactg ttttatccaa gtcggcattt gcggattcgt
      901 tagcgttttt tcgctcttgc tgcaaacgca cgacccacca tgccgacttg tgctgaaaat
      961 aaaaaagtgg acaaaaattc atccactcat tcttccgtca cgtccaaccc caaaagccac
     1021 gtatccaatt cttttttcaa aacatctgcc gattccgaaa atgtctctcg taaatgatta
     1081 actaactcat cactttggct ttcttttttc aacaaatttc gatatttata ttttgtatga
     1141 cgagccaagc cagcccataa attttgatct gaaatcaatc ctgcaatcat aattctatca
     1201 gtatgctttg tatcagtcgg caaaaaatga ggacttgcga aactgtctaa actttcatga
     1261 accatcgcat accaatcagt agcttttcca cgccgtaact gcaattccag tcgccaccaa
     1321 gtttttattt ccggtggtac aattttccgt ttggtttctt gttccaattt tttgttgtac
     1381 atccttattt gtcgctcact ggcacgacta ccccaatatg ccgtttccag cttcccagac
     1441 cgcccataga ttggtttgaa tgaaacaggg tcaaccacac gatattgcgt aataaaatca
     1501 tcaggcacat cgataatgtc gcaagcaata tcagcacgtg agaaatgtgg ttctaaaaat
     1561 aaatcatgga taaagttctt catcgaaccc gccaaaaatt ggctaatttt atttgggtta
     1621 aagtcaattc gcccttgccc ttcttgatat ttcaaaattt caatgtaagc aatattctca
     1681 tcatgcttat ccacaatttg ccaaccattt agaccaacag atttaacata accattattt
     1741 tcattcagtc gcattaactg ttcaaagtct aaaatcaata catcattttg cgtgtgataa
     1801 taaatattct ctttgagttt tccaacaatg gtaattcggt caattgacca ctgtatttta
     1861 aggggggtgg tcaaagtttg cacacttaac cgcctgttag atagggctgt taaattttcc
     1921 cactcattta tgatcgtgcc acgtttactc aaattttgtt cctcctcaaa gaaaaagaga
     1981 cttttggaaa gaatttccag agttcacgta accctaaaaa ttcatatcca aaaatctcta
     2041 gcttgaagaa acctaaaaat attcgtataa tgaacctgag ttacggagct aggtgaagta
     2101 gtttccgtaa atagtcgaga gtgttagcgc actgctcggc ttttattttg ctcttttttc
     2161 tcgttttctt tgattatagc actcctcct
//
LOCUS       DANKEF010000162         2082 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000162 DANKEF010000000
VERSION     DANKEF010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.172"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..191)
                     /locus_tag="PWZ48_002999"
     CDS             complement(<1..191)
                     /locus_tag="PWZ48_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2262577.1"
                     /translation="MNHFKGKQFQQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSH
                     TTIYRWVQEYGKLLYQIWK"
     gene            519..746
                     /locus_tag="PWZ48_003000"
                     /pseudo
     CDS             519..746
                     /locus_tag="PWZ48_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            872..1843
                     /locus_tag="PWZ48_003001"
     CDS             872..1843
                     /locus_tag="PWZ48_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDL2262578.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            1939..>2082
                     /locus_tag="PWZ48_003002"
     CDS             1939..>2082
                     /locus_tag="PWZ48_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HDL2262579.1"
                     /translation="MENKTETFQKIGGTSMKRRMLAIAAILALTAGGLAGCGSKEKSS
                     GSSD"
BASE COUNT          687 a          332 c          410 g          653 t
ORIGIN      
        1 tttttccaaa tttgatagag tagtttgcca tattcttgca cccaacgata aatcgtcgta
       61 tgagaaacgt taatgccacg atcatataag atttcttgaa cttcacgata gctaaggtta
      121 taacgaagat agtatcccac ggctacaata atcacatcct gctgaaattg ctttccttta
      181 aaatgattca tcgtcattcc tcctgctatc tttttctatt attctacctt atttgatagt
      241 agatttaaaa ctttgcaaca gaaccacatt aattttgttg gcaaatatag tttccgctat
      301 gaaaaagaat tatcgaatgg aatactacgt ccgttaaata tagagaatta ttgagaatgt
      361 tcgtcttttg gttcctaccg tgcattttcg cacggatgtt ccagatacta tttttgaatg
      421 tataaaattg aaaacacaag aatcctatta aatcaacgat tcttgtgttt ttaatttttc
      481 actaccgtgc attttcgttt ctatgttcca tgtaccccaa tcttgaaaaa ttaattaaag
      541 acttatataa agccattgac gacttagtcg tggtgctacc ggagtatcaa tgtttaacca
      601 gtatcccagg tattggtcct gtttaggctg ctggaatact cgctgaaatt ggtccaattg
      661 aacgtttttc tgatgaaata aaattgacaa agtatgccgg cctatattgg cgacagagtc
      721 aatccggtaa atctgaatac gaaaattttt ttacgaaaag cttcacgtct aatatctatt
      781 ttaaaataaa aatattcctg ctaatttgga atcgtttcct tcttttggta taataatacc
      841 tgaaattctt ttgagaggag ttgttcgtga agtgaaaact actatgaagg atgttgctgc
      901 gttagcagga gttggtgtgg gtacggtatc tagagtaata aatggagtaa aggtgaaaga
      961 ggttaccaaa gaaaaagtaa tggctgcaat tagtgagctt aattatgagc ctgatgaata
     1021 tgccagaggt atgaaaacga atagcagcaa tacaatagct cttattttac ctacgatttg
     1081 gcatcccttc ttttccgagt ttgcctacta tgtagaggaa gctctcagcc aaaaaaatta
     1141 taagcttttt ttgtgtaatg cgaacggcag tgcggaaaaa gaaaaagaat atatccaaat
     1201 ggtcaagcag agtaaagtag acggaattat aggtattacc tattcagaca ttgataaata
     1261 tgtatcatca aatttaccat ttgtcagtat agaccgccat ttttcggaac aggtttctta
     1321 tgtaacggca gataattatt taggtgggca aattgccgct caagaactac ttactcgtaa
     1381 ctgtaaaaac ttgtgctatg ttgggggtgt ttcaccttac cctaacgaaa caaacaatcg
     1441 taaagctggt ttttacaata tttgtgagga aaataaaata gctgtgaagg tattggatat
     1501 gcctgaacca attagcaatt tggaggaaca attgctcgct tttttttcgg ataatcctga
     1561 aattgatagc atttttacca tcaatgattt catggctttg gatgtgataa aagcccaaaa
     1621 aaaactaggg aaggaagcac ctaaggattt tcaaattatt ggttttgatg gagtcaagct
     1681 atcgttgcaa aaagactatt tactatcaac gattgtacag ccgatagagg aaatggccaa
     1741 ggtttcagta gaaacactca tggcgatgat tttaaatgaa gaaccgccta agcgtgtggt
     1801 agtaccggtt ttctttcacg ctggagaaac gactaaagag taaaaaaccg gtttataacg
     1861 attttttttg agataggttg acagaaagtg gtttcaatga tatcttaaaa ccaattaatt
     1921 ggaaacgtta ccaattaatt ggaaaataaa acggaaacgt ttcaaaaaat aggaggaaca
     1981 tcgatgaaaa ggagaatgtt agcaattgca gcaattttag cattaacagc tggaggactt
     2041 gcaggatgtg gcagtaagga gaaaagttca ggcagcagtg at
//
LOCUS       DANKEF010000163         2056 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000163 DANKEF010000000
VERSION     DANKEF010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.173"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            493..714
                     /locus_tag="PWZ48_003003"
     CDS             493..714
                     /locus_tag="PWZ48_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262580.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            715..972
                     /locus_tag="PWZ48_003004"
     CDS             715..972
                     /locus_tag="PWZ48_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262581.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1108..1710)
                     /locus_tag="PWZ48_003005"
     CDS             complement(1108..1710)
                     /locus_tag="PWZ48_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HDL2262582.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQIREITQKLRSPNPQKCANAKGMVRYFAHM
                     DNPEKFQYAKSDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDLIDYA
                     MSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
     gene            complement(1777..1932)
                     /locus_tag="PWZ48_003006"
     CDS             complement(1777..1932)
                     /locus_tag="PWZ48_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2262583.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
BASE COUNT          678 a          462 c          314 g          602 t
ORIGIN      
        1 aaaaaaaaac gctgaaaccc ttgggagagt aaggctaaac agctatcgac caatgaccaa
       61 tgaccaataa ggggggtgtc gtagtccccc cctcctccgc ccaattcata aacaccaaaa
      121 ccaaaaaaca aacctcaggg ctccgccccg aaccccgacc tccttctacc gaaatatatg
      181 caggcgccac gcttactatt tcgttcagct atcggctttt gcaatctaac acgaccgttt
      241 cgctaccagt cgccttacag gctccattct cgcttctctt acgtgtttta acgattgcgc
      301 cgataactaa acgaaataga cgctaaatcg cttaaaacgc aaaatagacc ctttttagag
      361 cattgatttt ttctactagt catttcgata atataaaatt gaccacaaca cagaaaactt
      421 atattataag cttatgtcgt aagtccgacg tactcacgta atatagacgg acgttaaatg
      481 gaaggagcat aaatgagagt gataaaatca gaaaaattca ctttggccga aattatgacg
      541 accagcgaac ttgcacaaga acttgattac aaccaagctt acgttttgcg cttagcgaaa
      601 gaacatctga ctgaaggaaa agaatatcgc tctgccggta gaagaaacta tcttttcagc
      661 ccagaagccc ttaaaaaact agaaaaagtc ataaccacca aagaaggtga gtaaatgcct
      721 aaagaaacaa ctatcaacgt tgaacaacta gaaaaattgc tagaatcata ttccaatgat
      781 tttatcgaat tgaccggcgg aaaagtcgaa gaaaccaccg acctctttga cctacttctc
      841 acaaatattc tggaattaaa ctctgaagaa caaaaagagc ataagggaaa actgatagca
      901 ccttcctaca ggaaggtggc taagtcggct gtgccaactg gttttcttac aaaacaacct
      961 tatatttttt gatatcacaa aaaaatccat tttggaccta ttttcggtca taatatagta
     1021 cctacttttg gtcatagttt cgtccgtagt tttggtcata gtgactaaga ctttcacgca
     1081 caaaaaaaga gctgaaatta acagctctta tcctttattt atttttgaat ttacacttcc
     1141 accatgacga ttcgatttta tatattgccc gataatataa gctgaattat cacaaagaag
     1201 cggaaaccaa tcatcaaatc tttctgacat tgcatagtca attaaatctt tgatttcagt
     1261 gatattctta gaatcaacaa acgacatcat ctctctaatc aactcatatc gttctgcact
     1321 cgttactgac aaataactag caatttctgc accaccatga gcgataatat cacttttagc
     1381 atattgaaat ttttctggat tatccatatg agcaaaatat cgcaccatac cttttgcatt
     1441 agcacatttt tgcgggtttg gagaccgaag cttttgagtt atctcacgta tttgatcata
     1501 cgtcttttta ccgctaaaca ttatcaaaac atgccaatga ggctttttca attcaccgtc
     1561 aggattcaca tctttatcat gaagtggact ctcaacccaa ggaacatgta actcatctaa
     1621 aaactccctc caattttcag gagccgattc aggatagact acaaacgtcc aattccttgt
     1681 gcgttcatct ttagtttttc tactcgtcat gctataatta ccttaattga acaacagtct
     1741 aacagcaaaa gcagtgcgcc aacactaatg cttttgctat tttttttgct cattttcctt
     1801 tccgttagca ttaacccaaa ttgtaataag accagactta ctaagaccca tttgtttcgc
     1861 aattttatca agattatctg ccacttcctt agtaagcgtt acaacgactc tctttttttc
     1921 attactgatc ataattcgta tcctccaacg actttttctt aaaaaccata gtaacaaaaa
     1981 agtaactaag gtgcaacagt ttagatacta attagcgaat aataggcctt aaacccttgg
     2041 cattactgat tttgtc
//
LOCUS       DANKEF010000164         2005 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000164 DANKEF010000000
VERSION     DANKEF010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.175"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            178..1284
                     /locus_tag="PWZ48_003007"
                     /pseudo
     CDS             178..1284
                     /locus_tag="PWZ48_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            1606..1815
                     /locus_tag="PWZ48_003008"
     CDS             1606..1815
                     /locus_tag="PWZ48_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2262584.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            1808..1957
                     /locus_tag="PWZ48_003009"
     CDS             1808..1957
                     /locus_tag="PWZ48_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDL2262585.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
BASE COUNT          718 a          319 c          355 g          613 t
ORIGIN      
        1 gtagtgatag tgcaacatta acggctaact tggatacgaa agaatttatc attgagtcta
       61 aagatataaa aattttcact agtgttgcat taaaatttta acctattcca tagaacaaaa
      121 aatcctttat actagcactt ggatgactta tccaagacca ataaaaagga ctcatacatg
      181 gataagtata aaacaaatac agcatttaat aaatggtttt ctgccataaa attaggaaaa
      241 ctttctgtca actcccaaaa atgtattttt gattacaatt cgtatcgtaa aaaactgtct
      301 tttgaagctg tgctcaagct ctttctctac gcagttaatg aagaaaaaga aagtttgcgt
      361 gatttgagta cagctcttat cgaccgatcg ctccaactag aaaccgatgt gtcatcgatt
      421 agtcataccc aattgtctcg agcatttaat gcacttgaac ctaaagtgtt agaagaaatt
      481 tttcaacaac tgttagaaaa agttcaacat caaatcaaac caaccaagcg aaatcatctg
      541 tatctgattg attccagtac gttttcgctt agtttaaaac gtcataagtg ggcgactttc
      601 cgtcaaacaa aatcaggtgt caagttacac ttaaactatt gttatatgga cgatactacg
      661 atgtatccaa ctgattttac cttaacaaat gcccatgaac atgatctcaa tcaactacca
      721 gtattggtga atcaaccaga ggcaacttac gtgtttgatc gtgggtatta ggactttgaa
      781 aagatggatc agatgcattg ggatggctat ttcttcgtca ctcggattaa aaaaaacacc
      841 aaagttcacg tgatagatac tctggaaaca tcgccagaaa cagaaattct acgagacgag
      901 ctggttcgtt taggatctaa aacctatcta accgccaatt tccgactagt cactgttcaa
      961 gataagaacg gacgtgtgtt tcaatttatc accaatcgaa tggacgtctc ttcaaaagaa
     1021 atctctgata tgtatcacgc tcgttggcaa atcgaattgt ttttcaagca catcaaacaa
     1081 cacatgacca ttaaaacctt cttttctcaa agtgaaaaag gcgtgcagaa tcaattgatt
     1141 ttaacgatga tttccgctct cctcacgttt ctcatcaaat tggagacgaa aacggaaaaa
     1201 tcagtgttcc aaatcaaacg attctttcgt tatttactgt ttcagccctt tgaatgttgt
     1261 ctagaaaaat taattcccac atgagaaaaa agaaatttgg ctcgttactt ttggttttca
     1321 gttgaataac agataaaaaa ttttctggaa aaagtcatcc tctgcgtcag cacagttttt
     1381 tttgacttat tttcagagga aatagttttc agaatattct aaaatgtttt atgcaacact
     1441 agtgatattt tttagaatta gatttctaat gtgcattttt tgagtattaa ggagcaagta
     1501 tacaaagatt tataaagctg attaaatttg aataaaaata tgcaggaacg ctactatttt
     1561 tagtagtgtt cttttttata tttgaataaa aaatgtggtg gttaaatggt ggttataaca
     1621 aaaaatgaat taatgaaatt aggttatggg aagtatcagt ctgagagaat catacgacag
     1681 gcaaaagcaa ttatggtttc aaaaagctat acctattacg aaaatagaag attaggacta
     1741 gtacctgttg aatctgtaga agaagtttta ggtatgaaga tagaggtaga ggaggatttt
     1801 cagaatcatg gttaatttta aaaaagataa aaatgggaaa tatcatttta ctgctagttt
     1861 gggattcgac aaacgaacag ggaaaagagt acaaaaaaac gtagaggggt taaaacagtt
     1921 aaagaagaaa aaaagcgtat accgaacttg tttctaatta tggaaaagaa gcagaaactt
     1981 ctcaaagtac tatgttgttt gagga
//
LOCUS       DANKEF010000165         1995 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000165 DANKEF010000000
VERSION     DANKEF010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1995)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1995)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1995
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.176"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..741)
                     /locus_tag="PWZ48_003010"
     CDS             complement(<1..741)
                     /locus_tag="PWZ48_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HDL2262586.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGICYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEA"
     gene            complement(745..1803)
                     /locus_tag="PWZ48_003011"
     CDS             complement(745..1803)
                     /locus_tag="PWZ48_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HDL2262587.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
BASE COUNT          641 a          425 c          328 g          601 t
ORIGIN      
        1 agcttcatta tcttgaagct ctccacgggc tgtattgatc aagatgctgc cttttttcat
       61 ttgagctaaa aatccttcat tgatcatttg atcgttttca cctgggatat gaggtacatg
      121 aatactgacg atatcgcttt tggctaataa ctcttcaagc gagtggaacg tcaatacttt
      181 tttagcagct tctgtctgat gaatatcgta gccgataact ttggctccta gtcccttaaa
      241 caaaacagct tccgtcaacc ctatcctgcc tgttcctatg atccctactg tgcaatttct
      301 gatttcacga ctaaacattt gctcatccac tcgaaaatct ttgaaagatg ttcggatcgt
      361 cgtatacgca gtatttctca aaagcatcat ggcaaaagtc aaagataact ctgctatcgc
      421 atttggcgaa tacgatggta cacgggctac tgtcatacca aaatcagctg ccgcttgtag
      481 atcgatatga ttgactccca cagtccgtgt aaaaacataa caaatcccat attcctgcat
      541 ttttgcaaga ttggtctcat ctgctataca atttcccctc aacaatacag catcatggtc
      601 ctttgctgac acgatattgt catgtgtcaa cagttcttca taaagactga gatcataatg
      661 gtaaatattc aatttttcaa aaaaatctat ctcgttcgga cgaacaccgt aacatgcgat
      721 tctcatactt tcttcctcca tttcttatga aaataaacct atgccatgaa tcatttgtaa
      781 tacaacgatc catacaggtg tcagtaccac agcaaaaata gttgacagta aggatgcatt
      841 ggaggctaaa agtgcctccc tatcaaaact gatagcatag gctgctgcca ctgttgcagc
      901 tggtgtagcc atcatgataa cgattgttgc cagtgcttca aaattgactg gtagaatatt
      961 cgttgccgtc aaaactgcta gaatcacgat attcaatgca ggtacaaata ccactttgac
     1021 tagactgtaa taccatgaag ttttttcaga tgcagctttt ttgaagctta cttcacctaa
     1081 tgttgcgccg attgctagcc acgccaatgg tgaacttaaa ccagctaaat aatccatcgg
     1141 ctgaaatagc cacggtgcag tctggtcaat tcgcaaaaac gctacttggc ttatctctcc
     1201 ttctgcacct gtaactgaca cttgtggcag atacccttgg aacagccaaa tgaaaaggcc
     1261 ggcaaaagta gcgataatga ttggattgac gaacatcgct ttgatatttt tagcttccat
     1321 cttcaagcca ctcattttta tgtatccata agaatacaaa aagattcggt agccaatatt
     1381 gaaaattgag gaatacatca cgccgacagg accataaatc gcactgacga taggcgttcc
     1441 gaaaaaggtc gtagaaccaa agatcgtcag gacacgcaac gttgtttgct tatccccttt
     1501 atagcttaag aaaaaaggtt ttgaaactag aataagaata atataaataa ctaatcccca
     1561 aatcaagaca ttgattcctt gcatcagtaa ttctgtattg atatcttgca tgaaagctcg
     1621 aaatgccagt gctggcaaag ccactgttaa gacgactttc gatagaattt ttccaacaga
     1681 ttcagaaaaa atcccctttt ttctacaaac aaaccctaat aatataataa aaactgttga
     1741 agtgattgca ctgacaatcg acatatctgt caaagtcgat cgaaaaatgt caaataagtt
     1801 catctttctc ctcctaatta tgttcattta ttcacaataa aaaaacgctt ttattttgct
     1861 aatacgtatt aacattttta attttatctt tacattgtta cttattgaac aataggaaat
     1921 taaagaaact tttgaaactt atgtaaagtg atttttacta gaacacaaaa aaaaccttaa
     1981 aaccaaactt aagaa
//
LOCUS       DANKEF010000166         1993 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000166 DANKEF010000000
VERSION     DANKEF010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1993
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.177"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            178..1191
                     /locus_tag="PWZ48_003012"
     CDS             178..1191
                     /locus_tag="PWZ48_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HDL2262588.1"
                     /translation="MLGFSEERFLSVLNGAVKNIETINQVADAISEKGFKNLYLVGSG
                     GTYATISPLAYLLSTHSELDYYHEIAAEVVCSQPKKINKDTVLITSSLTGTTQETVDV
                     VKYANSQGATTIAFVGNAATPLGEIVDYAIENDADNDTLVEELQLQFFALGARLMKNN
                     GEFPEYDEFIQTLQKMPEVLLSVRQQNDQKALEFAEKHKNTEFHMFVGAGNTYGAAYE
                     YAMCVLEEMQWIATKSIHAAEFFHGTIEMTDKSMSFVLLKGEDQTRPLVDRVERFVNK
                     YSDVVTVFDTADFELAGVPSAMRQYVAPMVMATALERVSAHFEKVRDHSLDIRRYYRT
                     VEY"
     gene            1202..1612
                     /locus_tag="PWZ48_003013"
     CDS             1202..1612
                     /locus_tag="PWZ48_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDL2262589.1"
                     /translation="MRKIVIATHSMMAKGLKDTLEFIIGPQKSLLAITAYIDSDYSIN
                     QEIEQLFSDLAPDDELIVTVDLLGGSVSNAFSEQIGKDNFYLISGVNLPLLLELCLSG
                     EQDTKKLIQKAMNSGNEGIVLVNELLEKTEEDDF"
     gene            1612..>1993
                     /locus_tag="PWZ48_003014"
     CDS             1612..>1993
                     /locus_tag="PWZ48_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDL2262590.1"
                     /translation="MIKLLRVDERFVHGQVAFAWTNNLGANCIFVASNEIATNNLRKK
                     TLKMAVPTGTKFVVKDMTEAISVLNGEKIKKYQVFLIVGTVEDAYEITQHVPEIKEVN
                     LGNLKKNENRKMITSSVYLNEQELD"
BASE COUNT          665 a          298 c          449 g          581 t
ORIGIN      
        1 aaaaaaggat tttggtacaa ataaaaataa aagtggttaa aaactattga cagcgttttc
       61 tataagtggt acattaaaaa cgttaacagg tggtatataa aaaaggttaa gtggtttgtt
      121 aatgtgccgg catttgcaaa tatatttttc aaattattga cttggaggtt tttaactatg
      181 ttaggtttta gcgaagaacg ttttctatca gtattaaatg gtgcagtaaa aaatatcgag
      241 acaatcaatc aggtggcaga tgcaatcagt gagaagggat ttaaaaatct ttatttggta
      301 ggctctggtg gaacatatgc cacaatcagc ccgctagctt atctgttaag cactcattca
      361 gagcttgatt attatcatga aatagccgca gaggtggttt gttcacagcc gaaaaagatt
      421 aataaagata cagtgttaat cacatcttca ttaaccggta cgactcaaga aaccgttgat
      481 gttgttaagt atgccaatag tcaaggtgca acgacgattg cgtttgtagg aaatgctgct
      541 acaccactcg gggaaatcgt ggattatgcg attgaaaatg atgctgacaa cgatacatta
      601 gtagaagaac ttcagttaca gttttttgct ttgggtgcac gcctgatgaa aaataacggt
      661 gagtttcctg aatatgatga atttattcaa acattacaaa aaatgccgga agtattacta
      721 agtgttcggc aacaaaatga tcagaaagca cttgaatttg cagaaaaaca taaaaatacg
      781 gaatttcata tgtttgttgg agccgggaat acttatgggg ctgcctatga atatgctatg
      841 tgcgtactgg aagaaatgca atggattgcc accaagtcga tccatgcagc cgaatttttc
      901 catgggacta ttgaaatgac tgataaatct atgagttttg tgttattaaa aggtgaagat
      961 caaacgcgtc cgctagtcga tcgcgtagag cgttttgtaa ataaatatag cgatgttgtg
     1021 acggtttttg acactgcgga tttcgaatta gcaggtgtac caagcgctat gcgtcagtat
     1081 gtagcgccaa tggttatggc aacggcctta gaacgagtga gtgctcattt tgaaaaggtg
     1141 cgggatcatt cactagatat tcgtcgttat tatcggactg tggaatatta aggtaagagc
     1201 tatgaggaaa attgtaattg cgacgcattc catgatggct aaaggcttaa aggatacttt
     1261 agaatttatt attggtcctc aaaaatcact tttagcaata actgcgtata tagatagtga
     1321 ctactccatt aaccaggaaa tagagcagct attttccgac ttagcgcctg atgacgaatt
     1381 aattgtgacg gtagatttac tgggaggaag cgtgtctaac gctttctccg aacaaatcgg
     1441 taaagacaat ttttatttaa tcagcggtgt aaatctgccg ctgcttttag agctttgctt
     1501 atcaggtgaa caggacacga aaaaactaat ccaaaaagct atgaatagcg ggaatgaagg
     1561 gatagttttg gtgaacgagt tgctagagaa aacagaagag gatgattttt aatgattaaa
     1621 ttattacgag tagatgaacg ttttgttcat gggcaagttg cttttgcctg gacaaataac
     1681 ttaggtgcaa attgtatttt cgttgcaagt aatgaaatcg ctaccaataa tctgcgcaaa
     1741 aaaacactaa aaatggctgt tcctacaggg acaaaatttg tggtgaagga tatgacagag
     1801 gccatctctg ttttaaatgg agaaaaaatc aagaaatacc aagtattttt aattgtcggg
     1861 acggtggagg atgcctacga aattactcag cacgtacctg agattaaaga agtgaattta
     1921 ggaaatttaa agaaaaacga gaatcggaaa atgattacta gttccgttta cttgaatgaa
     1981 caagaactgg act
//
LOCUS       DANKEF010000167         1989 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000167 DANKEF010000000
VERSION     DANKEF010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.178"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            127..1851
                     /locus_tag="PWZ48_003015"
     CDS             127..1851
                     /locus_tag="PWZ48_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HDL2262591.1"
                     /translation="MKKKLLTLFLVMSFSIVLSVYYNTIVFSEQINYTEEQYNIEKEK
                     ALKVGYSEEQFKQIMEIPTNLSNENSETRIVNYTMTSNQTKVINKAMEQIGKPYEWGA
                     SGPNSFDCGGLVKYVYKQAVNIELPMGTTNQEQYGTEVSLNSLKPGDLLFYGNRGATY
                     HVGIYKGNGVMIHAPQPGETVKEVNIQYFYPNFAKRILPDEPDYPYIDYNKMVTVTKA
                     WSIWNDLQFSHEIKKAIIGDNYKIGKVYTNPENNNKYGEILVSNKVYGYINFYAVKEL
                     TSVQINRYLTSKDSGQPTWGNLECTISKGQTTKDKIYFVKGAYNLGDGKYLYSIYKDQ
                     DSSEWLGYLKAHVSLAYTPIEEINKNVTVTKNWSIWNNLQWEKEIEKPQIGSVFSARL
                     KLTNVSNNAVYYKLYKSGKFYGYINAEAVKDLTTTKLNKYVTFSVNNEDFWSSLDVNY
                     SKGKTERGRVFYISISYNTADNQPIYSVYTDETCTEWRGYYKGNNFEDTQITMLENKS
                     VKVTKSGYTVWGDLNFWTKSGISNTGDIYTTDRKFYNFTNNAYYYELKKDGRVYGYIN
                     SEAAVEMN"
BASE COUNT          780 a          241 c          347 g          621 t
ORIGIN      
        1 agtttgtaac aaacatatat tttttatatt acaatatttc aacaaattaa aaaaaattat
       61 atcttcttaa acaatgataa tattaaaatg caagcgcttg taaaaaataa ggagaagagg
      121 taaaaaatga agaaaaaact attaacttta tttcttgtga tgtctttctc aatcgttttg
      181 agtgtgtact acaatactat tgttttttca gaacaaataa actacacaga agaacagtat
      241 aatatagaaa aagaaaaagc tttaaaagtt ggttatagtg aagaacagtt taagcagatt
      301 atggaaatac ctacaaattt atcaaatgaa aatagtgaaa caagaatagt aaattatact
      361 atgacttcta atcaaaccaa agtcattaat aaagcaatgg agcaaattgg aaagccatac
      421 gaatggggag catcgggacc aaattctttt gactgtggtg gtttagtgaa atatgtttac
      481 aaacaagctg taaatattga actgcctatg ggaaccacca atcaagagca atacggtaca
      541 gaggtaagtt taaattcact taagcctggt gatttattat tttacggcaa tcgaggtgca
      601 acttatcatg taggtatata taaaggtaac ggtgtaatga ttcatgcacc gcaacctgga
      661 gaaactgtaa aagaggttaa catacaatat ttttatccta actttgcaaa aagaattttg
      721 cctgatgaac ctgactaccc ttatattgac tataataaaa tggtaactgt tacaaaagct
      781 tggtcaatat ggaatgattt acaatttagt catgaaatta agaaagctat tattggagat
      841 aattacaaga tagggaaggt ttatactaat ccagaaaata ataataaata tggagaaata
      901 ttagtaagta ataaagtata tggatacatt aatttttatg ctgttaaaga gttgacctct
      961 gttcaaataa acagatattt aacttctaaa gatagtggtc aaccaacttg gggaaatcta
     1021 gagtgtacta taagtaaagg acaaactaca aaagataaaa tatattttgt taaaggagca
     1081 tataatttag gggatggaaa gtatctatat tctatttata aagatcagga ttcctctgaa
     1141 tggttagggt atttaaaagc acatgttagt ttagcctata ctcctattga agaaattaat
     1201 aaaaacgtaa ctgttactaa aaattggtcg atttggaata atctacaatg ggaaaaggaa
     1261 attgaaaagc ctcagattgg ttctgtattt tcagctagat taaagttaac caatgtatcg
     1321 aataatgctg tctattacaa gctttataaa tctggaaaat tttatggtta tatcaatgct
     1381 gaggctgtca aagaccttac aacaacaaag cttaataagt atgttacatt tagtgttaat
     1441 aatgaagatt tttggtcttc tttagatgta aattattcaa aaggaaaaac tgaaagaggc
     1501 cgagtatttt acattagtat ttcatataat accgcagata accaaccgat ttattcagta
     1561 tatacagatg aaacatgtac ggaatggcga gggtattata agggaaacaa ctttgaagac
     1621 actcaaatta ctatgcttga gaataaaagc gtgaaagtaa caaaaagtgg gtatacagta
     1681 tggggagact tgaatttttg gactaagtca ggaatatcga acacaggaga tatatacacc
     1741 actgaccgaa aattttataa ttttaccaac aatgcttact attatgaatt gaaaaaagat
     1801 gggagggtct atggctatat aaatagtgaa gcagctgtag agatgaacta aaatattgct
     1861 gtcccttaat cttatggggc agcaatattt ttatattatt aatcattttt ctgaagcgag
     1921 ggtagaagaa ttaatcaata gtttttcttg ttttgtaata taatagttta attaccaaaa
     1981 attatttaa
//
LOCUS       DANKEF010000168         1905 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000168 DANKEF010000000
VERSION     DANKEF010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1905)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1905)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1905
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.179"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(287..360)
                     /locus_tag="PWZ48_003016"
     tRNA            complement(287..360)
                     /locus_tag="PWZ48_003016"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(324..326),aa:Ile,seq:cat)
     gene            complement(549..1568)
                     /locus_tag="PWZ48_003017"
     CDS             complement(549..1568)
                     /locus_tag="PWZ48_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HDL2262592.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RSDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            complement(1579..1785)
                     /locus_tag="PWZ48_003018"
     CDS             complement(1579..1785)
                     /locus_tag="PWZ48_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HDL2262593.1"
                     /translation="MNNRTFDILKWIAIVVIPALATFVGLVGKGVNWRYTDITVMIIT
                     GFGAFLGSILGVSNRTYKKISSDK"
BASE COUNT          561 a          389 c          324 g          631 t
ORIGIN      
        1 ataaaagagt ttaaagcgta acacacttat ggggaagtgg cttggggtgc gctttaaact
       61 cttctttatt atcatctcac aatttaaccc aaatgtcttt ctatttaaaa atcaaagtaa
      121 aacttttcaa atatacagaa atataactat gtgaaacatc ctttcattaa tccataaaag
      181 gatacataaa aaagccactc atttgagtgg caatgaagaa aagctttagc ttgtataata
      241 ctcttcaaaa aattctaaca cagaacgatt caaatggcta cgttaatgta ccctgtagga
      301 ctcgaaccta cgaccggacg gttatgagcc gtctgctctg accaactgag ctaagggtac
      361 tggttgttgc cacataaagc cataaacaat caaccagtag aatgtgtggc aacaaacctg
      421 ttatcgcata gcttggagtg tgactattat gggtgatagt gaagatatgc gataacatca
      481 ctattttatc gaatgatttt tatagttgtc aatatagtta tgtaatgctc ctcaacgagg
      541 agctattttt atcgtttagg aatatttaaa taccaacgtt tgtcatggaa atcttgcgca
      601 ccgcctttag tgtttccctc tggatcattc gttgcccgca tcatgacata gactttctta
      661 ttagggaagt tacgcatgtt aaaagataca tgatagccaa catttccaga agtattataa
      721 gcttgattta catctgatct ataaattcca tcagctctta ctcgagctaa ttctttccca
      781 gtattgtaat ccataatgaa gatatactcg tatttatagt tagcaatgtg ccatccagct
      841 acatgcaaat ttgcattttc gatttctcca aactgatcaa tgtgggcgta attcgttcca
      901 tctgtcagtg taggattagc tgcaccagct cgtgttggat caatgacagg cttgttttca
      961 gaagttgttg gattttcatc ggtaaatcca tgagctaaat catatgctaa tttttcttta
     1021 cttacgccca tttcagaaag ataaccgtaa ggatctgtat gatcgcccca gatgttttgt
     1081 gttacccata aatgcgattt gattcccggt tggttataag gagtgtccaa cgttaatgga
     1141 ataccatatt tcattgctga atctctagcc aattcaacgt atgccttgta gtttttctca
     1201 aacgttgctt tatcatgtgt gtgttgtaac tcaatctgca caggactgtt ggcattagca
     1261 tacgaaccag caccgtactg tacataacca ggttgaccga cttgataaac aattccgccg
     1321 tctcccacaa tataagcagt ataagcgcta gtccatgaac gttgcatata ctgcgcttca
     1381 ttgcgtcctg ttgctgtttc attagccgtt tcatgcagta aaatatactg attatttgct
     1441 acttgtgagc taccttcatt tgcgcccaaa ttaaattcat tgttgatagt ataggcaaat
     1501 ccgttaattg gcaataaaaa aagagccgtt aacaggctca tcgcagtaat agtaattttc
     1561 tttttcatat ctatcctcct atttatctga tgaaattttc ttatacgttc gatttgatac
     1621 acctaaaata ctccctagaa atgctccaaa acctgtaata atcatcaccg ttatatctgt
     1681 gtaccgccag ttaacacctt ttccgacaag accgacaaaa gtcgctaaag cggggataac
     1741 tactatagcg atccatttca aaatatcaaa ggttctattg ttcatactat ccacctccct
     1801 tcattggttg ggctactttc tttttcaatc gcttgttttc ttctatcaat atttctcttt
     1861 ctgaaagcaa ggcatcaatt tgcccgtcca gttgttcaat tttat
//
LOCUS       DANKEF010000169         1847 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000169 DANKEF010000000
VERSION     DANKEF010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.185"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="PWZ48_003019"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="PWZ48_003019"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDL2262594.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DANKEF010000170         1832 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000170 DANKEF010000000
VERSION     DANKEF010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1832
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.186"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            175..597
                     /locus_tag="PWZ48_003020"
     CDS             175..597
                     /locus_tag="PWZ48_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262595.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            607..810
                     /locus_tag="PWZ48_003021"
     CDS             607..810
                     /locus_tag="PWZ48_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262596.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            1021..1605
                     /locus_tag="PWZ48_003022"
     CDS             1021..1605
                     /locus_tag="PWZ48_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262597.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
BASE COUNT          648 a          253 c          323 g          608 t
ORIGIN      
        1 gtcacttttc tttttaaatg gcaatatttt ttgtaaataa atgtcaatca agaaaattta
       61 aacgattatg acattaaaaa tatattttat gaattttcta gtatataact tgatttatct
      121 gttaagattt attaagaaaa cgttttctta tttttgattt cgaggtgata gacaatgaaa
      181 aataagttta ttttatcttt aggtatttta actattggaa ttagtgtaac tggaactaca
      241 attcctacat ttgctgatag tacaaatgaa ccaactactg tagtagataa taaccaagaa
      301 ttaactcctt tagataggaa cggaatagcc attggtaacc caactaacga cttaactgtt
      361 actgaaaatt atttaatagt ttctccatta cttgctcgag cagcttctaa aacgatacga
      421 gtgacaggaa cgatgatttt tgataaagtt ggttggcctt ctagacctcc tcaatcacgg
      481 aattggaaag aaaaaagaaa tggttattgg tactatggaa aattgtattt acaaagttat
      541 aaatcagctg gaaacaattg gattgcgact tattcaggaa ccttaactag aaagtaggga
      601 ttttttatgc cagaatttgt gaacacacca aatttttatc aagatagcga tattctaagt
      661 aaaattaatt taccgaaaga agaatcagct ttacaagttc agcgaacttt acaggagaca
      721 caaaaaattc aaaaatcaat ttctaatcta gaagattctg aagaaaaaag aaaaaaaaga
      781 gaccaaaaaa catatcaaaa agaaaaataa tgattttaaa aaaagagtgt ctagtcgaca
      841 ttcttttttt ctaattgaaa gtgttcataa aatcacccat ttctggacac tttctttttt
      901 tgctttattt ttcaaaaaaa caattcattt catgttcata aacccataac aatatctatg
      961 ttcagttaaa ctatatggat aaagttatgt tacaataaaa ttaatgaagg agtgattttt
     1021 ttgaaaatag gatacgcccg ggtatctact ggtttacaaa atttggattt acaagaagac
     1081 agcctaaata aatttggttg tgaaaaaatt tttactgatc atatgagtgg ggcaaaaagt
     1141 aatcgaccag gattagaaat ggccattgat tttgttcgct ctggcgatac acttgtcgtt
     1201 tggcgcttag atcgtttagg tagaaatatg gaagatttga tttcaatcgt caatcgactg
     1261 aatgagcgtg gtgtgagttt tcatagtatt caagaaaata ttacgatgga caaatcaagt
     1321 tctacaggac agctcatgtt tcatttgttt gcggcatttg cagaatttga gcgtaatttg
     1381 atcctggaac gatccgcagc aggacgagca gctgctcgtg caagaggacg gtttggtggt
     1441 cgtcctgaaa aattgtctga acaagatttg gaattactga agactttagt ggatagcggt
     1501 acacctatta agactattgc ggaacggtgg aacgtctcaa gaaccaccat ttatcgttat
     1561 ttggcaaaaa tggaggagaa aaatggaaag acaaattctc attagttttt gtcatttaat
     1621 gctgaaaatt tttgccattt atctagaaaa gtgacaatag tatgagaatt ttttgtaagt
     1681 gtctactgat aacttattct caaatgttgt aaggatttta ttcatttgtt aatattaaag
     1741 aacggaactc caaaaaagtc cgtactctaa tttgcagtct ctcactcttt tagagtgaga
     1801 gtttttatta gattttcaaa ttaattagta aa
//
LOCUS       DANKEF010000171         1821 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000171 DANKEF010000000
VERSION     DANKEF010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.187"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..1031)
                     /locus_tag="PWZ48_003023"
     CDS             complement(<1..1031)
                     /locus_tag="PWZ48_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2262598.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1113..1802)
                     /locus_tag="PWZ48_003024"
     CDS             complement(1113..1802)
                     /locus_tag="PWZ48_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDL2262599.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
BASE COUNT          607 a          412 c          321 g          481 t
ORIGIN      
        1 aatacagtat tatttcggtt gcttgctgct tcacctagcc cttgtactcg tgtttgattg
       61 gcgacttgac caaattgatt cccgacgtct aataaaataa tagctaaaag caaaatggcc
      121 aaatggcttc cgccaaccaa taaagcaata aagcttattg tttgcataac aagtcccata
      181 tacacgattt ttctttcaga ataagaatct gccagtcgtc ccacgatagg tgcagcaact
      241 gctccagaca aaccaaaaat cgctaatatc cctgcttcga atgttcccca attgtaggcg
      301 ggactgctga tataaaaaat caacgtagac caaaagatcg aaaatgttcc aaacatgaaa
      361 aatccactga ctgccgcttc tcttaataga cgttgagaag caactaaatg cggcaaactt
      421 ttaagtgaat caaggtatga aacagctgta ctactttgct ggacagaacg cggcattttc
      481 cagtaaagca gaagtgtaag aattccgact gcacataaag caaataaata gatcatccgc
      541 caagaaaaaa gactggcaac aataccagat accgtccgag aaagcaaaat ccccacgagt
      601 aaaccgctaa gcaatgtacc catgaccttc cctcttgctt ttggtcccgc tagtaccgca
      661 ccgtaaggaa tgatgatttg cggcacgatc gaaagtaatc ctattaaaaa agaagcgcat
      721 gcaaataacg taaacgaagg cgcaaaaaaa gcggccaaca gagagacggc agataacgct
      781 accattcgaa tgatcaattt tggacgatcc accacgtccc ctaaaggaac taaaaacagt
      841 aaccctaacg catagcctaa ttgggtcaac atcgtcaata tcccgactgc acttgtgcta
      901 acagataaac tagctgctac ttttgttcca atcggctgga tgtaatacat attggcaaca
      961 acaacgccac aagtgattga taatagtaaa ataagcgaag ttgttaaagg tgttttttca
     1021 ttactcttca ttgatttccc ccatttccta tcttagttta caacttatgg aaaagattgg
     1081 aaatagaaaa atacaactat tcgccacact tattatttgt gcttttgttt tccttgtttt
     1141 ttcttgcgat ctttataaat gaagacaact aaccctacta gcacagtaac acttgcaacc
     1201 aacgtgactg cagttttgtt atatccgtga aacagagctt ctccaccaca cgcatacaac
     1261 acagacgaag ggatgactcc aatcacgatc gacaatatga tttgctgtag aggcaatttc
     1321 atcgcatctg cagcaaaatt gaccatagaa gatggaataa ccggtatcat atagcctaac
     1381 attacaccta tttttggatg tttcatttgt cggattgctt gcacccaatg attcgtttcg
     1441 gttttcttat ctaaaaattt taagcgatgc ataaggaaaa ttgcagtgat gttaccaaga
     1501 gcattaccca aaatgttgat gatcgcacct gtcagtggtc caaagctcaa accagcaatg
     1561 actccaacta ctgaagtagg aatacctgga acagcacaca tcaccgctgt caatacgatc
     1621 aagagtatcg atgcaaaagt cccatgcgaa cgaatatgtg cgagtagctc tgccttactc
     1681 gctctcggat ttaaaaataa ttggatgtct gtctgatatt ctaaatacaa ccgatagccc
     1741 actaccagta tcagaatgat tcccaacgag atcaacatca atcggattat tttctttttc
     1801 atatttcctc ctgaaaaaaa t
//
LOCUS       DANKEF010000172         1788 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000172 DANKEF010000000
VERSION     DANKEF010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.188"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            113..778
                     /locus_tag="PWZ48_003025"
                     /pseudo
     CDS             113..778
                     /locus_tag="PWZ48_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element IS1182 family transposase"
     gene            814..1059
                     /locus_tag="PWZ48_003026"
     CDS             814..1059
                     /locus_tag="PWZ48_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HDL2262600.1"
                     /translation="MIDVNELLEEAIRETENLNDGEVFLVKDLFKGYVGNRIPRKDRL
                     LLGTLFLNHVSKMNGNLKAIEKTSSNQQRYKKTIDKF"
     gene            1162..>1788
                     /locus_tag="PWZ48_003027"
     CDS             1162..>1788
                     /locus_tag="PWZ48_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2262601.1"
                     /translation="MVDNIIKSVAEKLSSLSYIEGIVLGGSRARGTHTEDSDIDIGIY
                     YNSESFDINTINQFATKLDDEHRNNLVVPPGAWGDWINGGGWLVINGYHVDLILRDIK
                     RVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
                     PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQV"
BASE COUNT          612 a          257 c          356 g          563 t
ORIGIN      
        1 catttagata gtagtggaaa gacctctatt tatcttttga aagtgaatac aaaaataaag
       61 cacactctgc tataatatag gtgattaatc catttgagag aggtgcttta tcatgtatca
      121 aaattatacc ataaaccaac tttgtttgcc tatagatctt gaaattaaat tagaagaaaa
      181 tgattttgct catgccattg tgcagtttgt tgactctatt cccgatgaag tctttcttcc
      241 ttattatcaa tctatgggta gaccccaata tcatcctcgt atgctacttt ccattattct
      301 ttgtgcctat attcaagggg tttattcggg ccgccaaata caaaatatgc tgatagatag
      361 tattcggatg cgctacttat ctcaagagca attccctaac tttcgtacta ttaatcgttt
      421 tcgcgttcat ccgattatga acgatattct agatcattca tttgttcaat ttcgtgagct
      481 actggttcaa agtggcttaa tcagtggcag tgctctatac attgatggta caaaaattga
      541 agcggatgcg aataaatact ctttcgtttg gaaaaaaagt attttaaagt ataaggatag
      601 ccttgatcaa aaagcccttg aaaactacca acaaatggtg gaaacaaaaa tcttacctga
      661 gttaatagat gatttaaagg atgaactttc tatagaagag attgaaagga ttcgtcaatc
      721 tttggaagaa aaggaaaatc aattaattga agcgattgac caaaccgaaa cagtagaagg
      781 gggctgaccc attattaata aggagtggac aacatgattg atgtaaatga gctattggaa
      841 gaagctatta gagaaactga aaatctaaat gatggcgaag tttttcttgt taaagattta
      901 ttcaagggat atgtggggaa taggataccc agaaaggatc gacttctgct tggaacgtta
      961 tttttaaacc atgttagcaa aatgaatggt aatttaaagg ctattgaaaa gacttcatct
     1021 aatcagcaga ggtataaaaa gacaattgat aagttttaac tatcttttgt gaaattaaaa
     1081 aaattataaa tatgttgagt agaagcaaaa gatttggctt ggaattttta cagcagtgaa
     1141 tcgataaaag gagcatgctt gatggtagat aatattatta aatcagtagc agagaaatta
     1201 tcctctctgt cttatataga aggtattgtt ttaggtggtt cacgtgcaag gggcacccat
     1261 acagaggatt cggatataga tatcggcatc tattacaatt cagaatcatt tgacataaat
     1321 actattaatc aattcgctac aaagctggat gatgagcata gaaataacct tgttgtacct
     1381 cccggagcat ggggtgattg gattaatggc ggcggatggt tagtcataaa cgggtatcat
     1441 gtggatttaa ttttacgtga tattaaacgt gtggaacaaa taatgaaaga tacagagcac
     1501 ggaattgtta ctgccaatta tcagactggg catccccatg gttatattag tgcaatgtat
     1561 cgaggagaat tagcgattag caaaatacta tatgctaaga atgaaagctt atgcgaatta
     1621 aaaaaacagg cagaaactta tcccaatgct ttgcagaaaa gtttagttaa cttttttatg
     1681 tttgaagcag ggttctcttt aatgtttgta aaagcaaatt cgggaacaga cgataaatat
     1741 tatattgcgg gtcatgtttt tcgtatagtt tcatgtttaa atcaagtg
//
LOCUS       DANKEF010000173         1747 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000173 DANKEF010000000
VERSION     DANKEF010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.189"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(245..1108)
                     /locus_tag="PWZ48_003028"
     CDS             complement(245..1108)
                     /locus_tag="PWZ48_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2262602.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(1110..1382)
                     /locus_tag="PWZ48_003029"
     CDS             complement(1110..1382)
                     /locus_tag="PWZ48_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDL2262603.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(1399..1608)
                     /locus_tag="PWZ48_003030"
     CDS             complement(1399..1608)
                     /locus_tag="PWZ48_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HDL2262604.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          459 a          323 c          280 g          685 t
ORIGIN      
        1 taaatttgga aagttacacg ttactaaagg gaatgtagat aaattattag gtatagggca
       61 cctctaataa ctaccaatta attcacctca aaatgaaatt agaaaaaaac agcgaaattt
      121 ttaaatctat ttcttatcga tacaaattcc tcgtaggcgc tcgggacccc aacatgagga
      181 aatgttttta taaaaaaaga gtagttaccg ttttttggta actactcttt tcctttttta
      241 aggtttaaat acctggaagt ttaggtgttt tgggtattgg tggtgtaggt agctgcaagc
      301 tttccatttt ttgttgaatt gctttaaatt caggggtttc ttggtgttga ttttgaacca
      361 ttttttgctc tattctctcc aaagtcggtt gaatttcttt ccctgatact ttacgattta
      421 attctctttc taaggtctct ttcggactaa tggaaggcgt ttctaagctt gaatagagtt
      481 tcactccttc tctattgtaa agccttatat cgctaaataa gcccgtttta tgaagggttt
      541 ctaaattggt cggtaagttt ttgacaacaa tatcatgcgc ttgttttggt gttgccctag
      601 ctgtcattgg atcatctgca tacatggttt catatcgttc aatcgttcct aagtatgatt
      661 caattttcgg tactgccatg acgtatattt ttgtttcata acctttagat tgaagcattg
      721 tggctgtttg aatgggaacg tctgttgttc gtcctgttcc ttcaatcact aaattatagc
      781 cttgatcgct taaacggcta ataagtgctt ctgtcatgcg attcgaataa ggggtaacgt
      841 gttttactac gtctttttca tagagtttca ctaattcgtc aaaatttggg tgctgttgtt
      901 taaaggtatc attatcaatg acaataacat tcccttgtgt ttcttcaaaa attgctgatc
      961 gcaaactggt tttccctgac cctggttgcc caccaagtaa aaaagcggtt ggcgattcaa
     1021 ccgctagtct atttttagtt agtcgttcaa cattcttttc taaacgatct tcaaattgct
     1081 tttcggtgaa gtcagtaata ttcgccatgt taagccactt tctctttatt caaaatttgt
     1141 tttgaatatt gattcattga ccgccaatac tcatgaatag cttgtaattc ttcaaatggt
     1201 tgttgaaata aatcaacttc ttgtgccact tctaattgat ttaaaagatt cacttctagt
     1261 aaacgagtat ttttagtatc tagttcatga ttcaaaacat aatttaatac ccgattataa
     1321 acacttgccg ataactcatt aatgatttca atttcaaatg ttttttcata cgtaactgcc
     1381 atttcatttt cactcctttt aaagtttgtc aggtaaatat ttccgtatat attcttctaa
     1441 ggcttgatcc ataacttctt ttacgtttcc gccattcttt gctgtttcga tttttatgat
     1501 atggtgcaag tcggcacgaa cacgaaccgt cttatccccc attatatctt tttttgcgct
     1561 gattggcgta tcatttcgtt ttgctttctg tgcgcctaaa tttcccacga tcgctcactt
     1621 ctttctattt cttcttacta tttattttat catcacctat cacaaatcac aagtgatttg
     1681 tgattctatt ctttttcatt ttctaattga atgattcgct caatcatttc aaaaaatacg
     1741 ttctcat
//
LOCUS       DANKEF010000174         1739 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000174 DANKEF010000000
VERSION     DANKEF010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.190"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            1..1235
                     /gene="aph(2'')-I"
                     /locus_tag="PWZ48_003031"
                     /pseudo
     CDS             1..1235
                     /gene="aph(2'')-I"
                     /locus_tag="PWZ48_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1219..1611
                     /locus_tag="PWZ48_003032"
     CDS             1219..1611
                     /locus_tag="PWZ48_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2262605.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          716 a          133 c          297 g          593 t
ORIGIN      
        1 aatcattaaa aaaacattat acagagcctt gggaagatga agtttttaga gtaattattg
       61 aatataacaa tgttcctatt ggatatggac aaatatataa aatgtatgat gagttatata
      121 ctgattatca ttatccaaaa actgatgaga tagtctatgg tatggatcaa tttataggag
      181 agccaaatta ttggagtaaa ggaattggta caagatatat taaattgatt tttgaatttt
      241 tgaaaaaaga aagaaatgct aatgcagtta ttttagaccc tcataaaaat aatccaagag
      301 caataagggc ataccaaaaa tctggtttta gaattattga agatttgcca gaacatgaat
      361 tacacgaggg caaaaaagaa gattgttatt taatggaata tagatatgat gataatgcca
      421 caaatgttaa ggcaatgaaa tatttaattg agcattactt tgataatttc aaagtagata
      481 gtattgaaat aatcggtagt ggttatgata gtgtggcata tttagttaat aatgaataca
      541 tttttaaaac aaaatttagt actaataaga aaaaaggtta tgcaaaagaa aaagcaatat
      601 ataatttttt aaatacaaat ttagaaacta atgtaaaaat tcctaatatt gaatattcgt
      661 atattagtga tgaattatct atactaggtt ataaagaaat taaaggaact tttttaacac
      721 cagaaattta ttctactatg tcagaagaag aacaaaattt gttaaaacga gatattgcca
      781 gttttttaag acaaatgcac ggtttagatt atacagatat tagtgaatgt actattgata
      841 ataaacaaaa tgtattagaa gagtatatat tgttgcgtga aactatttat aatgatttaa
      901 ctgatataga aaaagattat atagaaagtt ttatggaaag actaaatgca acaacagttt
      961 ttgagggtaa aaagtgttta tgccataatg attttagttg taatcatcta ttgttagatg
     1021 gcaataatag attaactgga ataattgatt ttggagattc tggaattata gatgaatatt
     1081 gtgattttat atacttactt gaagatagtg aagaagaaat aggaacaaat tttggagaag
     1141 atatattaag aatgtatgga aatatagata ttgagaaagc aaaagaatat caagatatag
     1201 ttgaagaata ttatcctatt gaaactattg tttattgatg ataatatggt ttttgatatt
     1261 gataatttaa aaggatttct taatgatacc agttcatttg ggtttatagc taaagaaaat
     1321 aataaaatta taggatttgc atattgctat acacttttaa gacctgatgg aaaaacaatg
     1381 ttttatttac actcaatagg aatgttacct aactatcaag acaaaggtta tggttcaaaa
     1441 ttattatctt ttattaagga atattctaaa gagattggtt gttctgaaat gtttttaata
     1501 actgataaag gtaatcctag agcttgccat gtatatgaaa aattaggtgg taaaaatgat
     1561 tataaagatg aaatagtata tgtatatgat tatgaaaaag gtgataaata aatgaatata
     1621 gttgaaaatg aaatatgtat aagaacttta atagatgatg attttccttt gatgttaaaa
     1681 tggttaactg atgaaagagt attagaattt tatgatggta gagataaaaa atatacatt
//
LOCUS       DANKEF010000175         1653 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000175 DANKEF010000000
VERSION     DANKEF010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.192"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            18..1508
                     /locus_tag="PWZ48_003033"
     CDS             18..1508
                     /locus_tag="PWZ48_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015543625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HDL2262606.1"
                     /translation="MNIPFVVETVLHDGLLKYKFKNSKIRSITTKPGKSKGAIFAYRS
                     KSSMIGGRGVVLTSEEAIQENQDTFTHWTPNVYRYGTYADENRSYTKGHSENNLRQIN
                     TFFIDFDIHTAKETISASDILTTAIDLGFMPTMIIKSDKGYQAYFVLETPVYVTSKSE
                     FKSVKAAKIISQNIREYFGKSLPVDLTCNHFGIARIPRTDNVEFFDPNYRYSFKEWQD
                     WSFKQTDNKGFTRSSLTVLSGTEGKKQVDEPWFNLLLHETKFSGEKGLIGRNNVMFTL
                     SLAYFSSGYSIETCEYNMFEFNNRLDQPLEEKEVIKIVRSAYSENYQGANREYITILC
                     KAWVSSDLTSKDLFVRQGWFKFKKKRSERQRVHLSEWKEDLMAYISEKSDVYKPYLVT
                     TKKEIREVLGIPERTLDKLLKVLKANQEIFFKIKPGRNGGIQLASVKSLLLSIIKVKK
                     EEKESYIKALTNSFDLEHTFIQETLNKLAERPKTDTQLDLFSYDTG"
BASE COUNT          591 a          264 c          316 g          482 t
ORIGIN      
        1 gaaaggagaa cagctgaatg aatatccctt ttgttgtaga aactgtgctt catgacggct
       61 tgttaaagta caaatttaaa aatagtaaaa ttcgctcaat cactaccaag ccaggtaaaa
      121 gcaaaggggc tatttttgcg tatcgctcaa aatcaagcat gattggcggt cgtggtgttg
      181 ttctgacttc cgaggaagcg attcaagaaa atcaagatac atttacacat tggacaccca
      241 acgtttatcg ttatggaacg tatgcagacg aaaaccgttc atacacgaaa ggacattctg
      301 aaaacaattt aagacaaatc aataccttct ttattgattt tgatattcac acggcaaaag
      361 aaactatttc agcaagcgat attttaacaa ccgctattga tttaggtttt atgcctacta
      421 tgattatcaa atctgataaa ggttatcaag catattttgt tttagaaacg ccagtctatg
      481 tgacttcaaa atcagaattt aaatctgtca aagcagccaa aataatttcg caaaatatcc
      541 gagaatattt tggaaagtct ttgccagttg atctaacgtg taatcatttt ggtattgctc
      601 gcataccaag aacggacaat gtagaatttt ttgatcctaa ttaccgttat tctttcaaag
      661 aatggcaaga ttggtctttc aaacaaacag ataataaggg ctttactcgt tcaagtctaa
      721 cggttttaag cggtacagaa ggcaaaaaac aagtagatga accctggttt aatctcttat
      781 tgcacgaaac gaaattttca ggagaaaagg gtttaatagg gcgtaataac gtcatgttta
      841 ccctctcttt agcctacttt agttcaggct attcaatcga aacgtgcgaa tataatatgt
      901 ttgagtttaa taatcgatta gatcaaccct tagaagaaaa agaagtaatc aaaattgtta
      961 gaagtgccta ttcagaaaac tatcaagggg ctaataggga atacattacc attctttgca
     1021 aagcttgggt atcaagtgat ttaaccagta aagatttatt tgtccgtcaa gggtggttta
     1081 aattcaagaa aaaaagaagc gaacgtcaac gtgttcattt gtcagaatgg aaagaagatt
     1141 taatggctta tattagcgaa aaaagcgatg tatacaagcc ttatttagtg acgaccaaaa
     1201 aagagattag agaagtgcta ggcattcctg aacggacatt agataaattg ctgaaggtac
     1261 tgaaggcgaa tcaggaaatt ttctttaaga ttaaaccagg aagaaatggt ggcattcaac
     1321 ttgctagtgt taaatcattg ttgctatcga tcattaaagt aaaaaaagaa gaaaaagaaa
     1381 gctatataaa ggcgctgaca aattcttttg acttagagca tacattcatt caagagactt
     1441 taaacaagct agcagaacgc cctaaaacgg acacacaact cgatttgttt agctatgata
     1501 caggctgaaa ataaaacccg cactatgcca ttacatttat atctatgata cgtgtttgtt
     1561 ttttctttgc tgtttagcga atgattagca gaaatataca gagtaagatt ttaattaatt
     1621 attaggggga gaaggagaga gtagcccgaa aac
//
LOCUS       DANKEF010000176         1623 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.194, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000176 DANKEF010000000
VERSION     DANKEF010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.194"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>1623
                     /locus_tag="PWZ48_003034"
     CDS             <1..>1623
                     /locus_tag="PWZ48_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2262607.1"
                     /translation="VDENRAKQRLRIYYAKTSVIGNTIEVKAEPIFNDIRKSVLNKYD
                     SGTEKITATQAWQNAKVLAKPAIPSQFSFSSLVDTLANVKIEKANFLEFFGGKEGSIL
                     DRFHGEFLKDNNTLRHEKSLGTDHKIKAIYTKNLTGLDLEIDAQSVLVGVYPFISSSS
                     EGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAAKDWLKTNIDKQKP
                     QVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSARIVEYTFNVLTNS
                     YDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDIVDHQTDMITGQNG
                     GYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGINGTYETAWTLDGGFN
                     ASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKDIMELEARDYVSAD
                     AGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFRYSNASYVTSGDGS
                     SLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPG"
BASE COUNT          554 a          232 c          354 g          483 t
ORIGIN      
        1 gtagatgaaa atcgagcaaa acagcgtcta cggatttatt atgcaaaaac ttctgtaata
       61 ggcaatacga tagaagtaaa agctgaacct attttcaatg atataagaaa atcggtgttg
      121 aataaatatg acagcggaac agaaaagatc actgctactc aggcatggca aaacgcaaaa
      181 gttttagcga aaccagctat cccttcgcag ttttctttct cgtcattagt agatacgctt
      241 gctaatgtga agatagaaaa ggcgaatttt ttagaattct ttggtggaaa agagggatct
      301 attctagatc gatttcatgg tgagtttcta aaagataata acacattacg tcatgaaaaa
      361 agtctaggta cggatcataa aatcaaagcg atttatacta aaaacttaac tggtcttgac
      421 ttagagattg atgctcaaag tgttttagtt ggagtttatc cattcattag cagctcttca
      481 gaaggagaag acgagatcac tctaccagaa gaagttattt tcacggatta cgtggatgat
      541 tatcctgctg gatatgtttc ttttgttgat tttaaagaca aagcgactga tgtagccaca
      601 ttaagggaag ctgctaaaga ctggttgaaa acaaacatag ataaacaaaa accacaagtg
      661 agtggttcga ttgaattagt accattgagg catcaaagag gctatgaaaa atttgttgat
      721 ctagaaaaag tttcgatggg tgacggagta gatgtgtatc atccacagtt aaaagtgaat
      781 atgtcagcga gaattgtgga atatacgttt aatgttctaa ccaattcata cgataaatta
      841 gttgtaggaa acgtcaaaac aaacttctta gaaaacacag agaataatgt cagcaatttg
      901 attaatgatg ccattgatca attgaaaaac ggtggcgaaa tcagtgattt actcaatgat
      961 attgtagatc atcaaactga tatgattact ggtcaaaatg gtggttatgt tttattagat
     1021 cctaaagaag cgcctagtcg tattttgatt atggacacac cagataagaa taccgcaagg
     1081 aatgttttac aaatcaacaa tgctggtatt ggtttttcta aaactggcat taatggaaca
     1141 tatgaaacgg catggacgtt agatggcgga ttcaatgcct cgtttattac ggctggtgaa
     1201 atagtaggaa ttactattag aggtactaca ttaattagtg atggtgctga ttatagaaca
     1261 agtattgcta atggcaaaat gacttggtac tcaaaaaaag ttaacaaaga tattatggag
     1321 ctagaagcac gtgattatgt aagtgctgat gccggtattg tatcatacac catgaaaact
     1381 ggtggtggtt tcatgattag aaatccacag ggtaacttgg tttttagtac gtgggataat
     1441 ggtaataaca gaccgtttct atcttttggt gcgcccaatt tcaggtatag caatgctagt
     1501 tatgtaactt ctggcgacgg tagttcttta agcattaatg gtagtgcggg taactcatgg
     1561 gaatttaagg tagctggtag gactatgaaa tttactagtg atggtatgct aacgttacca
     1621 ggt
//
LOCUS       DANKEF010000177         1605 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000177 DANKEF010000000
VERSION     DANKEF010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1605
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.195"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..278
                     /locus_tag="PWZ48_003035"
     CDS             <1..278
                     /locus_tag="PWZ48_003035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262608.1"
                     /translation="IEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMVLSNV
                     WLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            385..>1605
                     /locus_tag="PWZ48_003036"
     CDS             385..>1605
                     /locus_tag="PWZ48_003036"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2262609.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIF"
BASE COUNT          491 a          231 c          281 g          602 t
ORIGIN      
        1 aaatagaaca aatgtatgaa attgtagtac ctgaatttgt tgaacccgtt ttagaacaag
       61 gtatcaagga taagtctttg gagattgaaa attcacatga aattgcagaa gttatcatgg
      121 tattaagtaa tgtatggctc aacccattag ttgaaatgac ggataaagag ggtatggaaa
      181 aacgttgcaa tacattcaat gcccttttaa atggaatagg catagacaca ttattagacg
      241 aagaaacaat ttctgcattt atacagtttt gtaaataact gtatttgtta tccctatacg
      301 atagactgct ctataaaaga gcagtttatt taaaccatta tataaaccga cggtctgttt
      361 gattaaataa aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa tcgtgatttt
      421 atacttgttg ttataggaca gataatttct ttatttggaa acgctatcct acgatttgca
      481 ttaccgtact atttgttaaa agaaactggt tcatcagctt tgtttggtat tgctacagcc
      541 tgctcttttt tacctatggt tattttgtcg ctggtaggtg gtattattgc tgacaggaca
      601 aataaaagaa acattatggt catactagat tttgtaacag caatcataat tttagtattt
      661 tatttgtcat tgggaaaaat tcctatcata cctttgttta ttgttgcttt gatgttactt
      721 tatggtattt ctggtgcata tcaaccagcg gtacaagcaa gtattcctct attggtatca
      781 tcagataaac ttaccgtcgg taatgctata ataaatcaag taaatacttt agcaaacttt
      841 atcggaccag ctataagcgg agtagttttt caattttatg gtatcaatcc cattttaatt
      901 gttagtatta tatgttttac tttttcagcc attatggaaa tttttatcca tattccacac
      961 caaaaaagac ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt aaaagatagt
     1021 tatacctttt tgaaaaagga gaaacccatt tttatatccg taatcttttt ggtttgcatt
     1081 ttcaatttgg ttttaagtgc tgttttaatt gttggtctgc ctatcttaat ttctgaagtt
     1141 ttaaaaatgc cagatacact atatggtttt tcacaatcag cattatcatt aggtggttta
     1201 tgtggtggta ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa cctttatacc
     1261 ttgttactta tttgttctgt ttctgtttta cttatgggaa tttcgctatt tttagaattg
     1321 cctgcaatag tttcctactg ggtagttaca gtgatgtgct ttgtggctat gggtgtatct
     1381 actatgtttg ttgttcaaat ttacactctt gttcagacac aaacaccacc gcaattagta
     1441 ggaaagataa tggctacgct ggtagcagtt gctatgtgcg gtcaacctat tggacaaatg
     1501 gtttacggta ttttattcga tgtatttata caagaaacat gggttgttat gtttgtagct
     1561 tctattgctt caatcatgat ttcaatatat tcaaaaagaa ttttt
//
LOCUS       DANKEF010000178         1600 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000178 DANKEF010000000
VERSION     DANKEF010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.196"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..567)
                     /locus_tag="PWZ48_003037"
     CDS             complement(<1..567)
                     /locus_tag="PWZ48_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDL2262610.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFTGETVIT
                     YGFGHLVELDSPDMYDEKWKQWALEHLPIFPNQYHYHVPKDKKKQFSVVKRQLQSADT
                     IVIATDSDREGELIAWSIIQQAGANQGKTFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEVQARQIADWLIGMNGSPLYSLLLQQ"
     gene            complement(578..>1600)
                     /locus_tag="PWZ48_003038"
     CDS             complement(578..>1600)
                     /locus_tag="PWZ48_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDL2262611.1"
                     /translation="ELKPKHERQNFLVGDDRLDHAVAFLWKDPQTGETVGASYQGTIV
                     DFNRFGKRGTYKHIDKNPTPNHGFNLKIGDPKHLKFFESSIDLLSYAALNREKLQDAW
                     LVSMDGLKHHVISHYVEESISELRRKQTFPQSIEICVDNDRAGHIFYEKEQMKGIVDP
                     FTNKKIRCERGIPNDWQVPKEYKATYEAVAKEMSVEPEAIMAIHKTETNLQLTNQLVS
                     AHDVQSTFGKMLAKGEPVETIDLKEACTTVAKELKVCERADGTYNFDRFYSRKANIKD
                     VNAGILLSYKAEQYYKGYKKHEHEFVPEVKKDWNDQLKHEIQQQEIRKQKRAMLFQQG
                     RQQERE"
BASE COUNT          400 a          353 c          276 g          571 t
ORIGIN      
        1 ttgctgcaag agtaaactat acagtgggct accgttcatc ccaattaacc aatcggcgat
       61 ctgacgtgct tgtacttctt caaattttgg ataggtctct tctgcgtctc gtaactgttg
      121 gaatccctga taaatggctt ctttttctaa ggagttgatc caaagtcttt taaaggtttt
      181 tccttgattg gcgcctgctt gttgaatgat tgaccaggca atcagctccc cttcacgatc
      241 gctatcagtc gcgatcacaa tcgtatcagc tgactgcagc tgtcgtttga cgacactaaa
      301 ctgctttttc ttatctttag gaacatgata gtggtattga ttggggaaaa taggcaaatg
      361 ttctaatgcc cattgtttcc atttttcatc gtacatatcg ggcgaatcta actcgactaa
      421 atgaccaaaa ccatacgtga tcacagtttc tcctgtaaac aatcgatctt ggatctcaaa
      481 atacccatct tttcgggtag ctttagaaaa gctatcggca taagccttcg cttgtgacgg
      541 tttttctgct aaaatgacgg ttttcatagt gacgacttca ctccctttct tgttgtctgc
      601 cttgttgaaa caacatcgca cgtttttgct ttctgatttc ttgttgctgg atctcatgct
      661 tcaattgatc attccaatcc tttttcactt caggtacaaa ttcatgttca tgctttttat
      721 agcctttgta gtattgttct gctttatagg aaagaaggat tcctgcatta acgtctttta
      781 tgttggcttt tctactataa aaacgatcaa agttgtatgt tccatctgcg cgttcacata
      841 ctttcagttc cttcgcgacc gtggtacatg cttctttcaa atcaattgtt tctactggtt
      901 cgcccttcgc caacatcttt ccaaacgtag attgaacatc atgagcagac accaattgat
      961 ttgtcagctg taaattagtt tcagttttgt gtatggccat aattgcttca ggttcaacac
     1021 tcatttcttt tgcgactgct tcataagtag ctttgtattc tttaggtact tgccaatcat
     1081 ttggaattcc tcgctcacaa cggattttct tattggtaaa aggatcaacg attcccttca
     1141 tctgttcttt ttcataaaaa atatgtcctg cacgatcatt gtccacacat atttcaatcg
     1201 attgaggaaa tgtttgtttt cgtcttaatt cagaaatcga ctcttcaacg taatgactga
     1261 ttacatgatg ttttaacccg tccatcgaaa ctaaccaggc atcttgtagt ttctcccgat
     1321 tcaatgcagc gtaacttaac agatcaatac tactttcaaa gaattttaaa tgcttcggat
     1381 cgcctatttt taaattaaat ccgtgatttg gcgtaggatt cttgtcaatg tgtttatacg
     1441 ttccccgttt accgaaacga ttaaaatcga cgatcgttcc ttgataactt gcccctactg
     1501 tctctcctgt ctgtggatct ttccataaaa aagcaaccgc atgatctaaa cggtcgtctc
     1561 caactaaaaa attttgccgt tcatgtttcg gctttagttc
//
LOCUS       DANKEF010000179         1573 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000179 DANKEF010000000
VERSION     DANKEF010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.197"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
BASE COUNT          594 a          179 c          320 g          480 t
ORIGIN      
        1 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
       61 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      121 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      181 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      241 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
      301 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
      361 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
      421 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
      481 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
      541 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
      601 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
      661 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
      721 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
      781 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
      841 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
      901 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
      961 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1021 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1081 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1141 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1201 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1261 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     1321 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     1381 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     1441 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     1501 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     1561 caaaggagtg taa
//
LOCUS       DANKEF010000180         1560 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000180 DANKEF010000000
VERSION     DANKEF010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1560)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1560)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1560
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.198"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            46..105
                     /gene="ermCL"
                     /locus_tag="PWZ48_003039"
     CDS             46..105
                     /gene="ermCL"
                     /locus_tag="PWZ48_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDL2262612.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            153..887
                     /locus_tag="PWZ48_003040"
     CDS             153..887
                     /locus_tag="PWZ48_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HDL2262613.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          583 a          246 c          211 g          520 t
ORIGIN      
        1 cttgtgctat aattattcta atttcataaa aaggagaatt aatatatggg catttttagt
       61 atttttgtaa tcaacacagt tcattatcaa ccaaacaaaa aataagtggt tataatggat
      121 tgttaatata aacctcatag gagtgggtta taatgaacaa aaaaaatata aaagatagtc
      181 aaaactttat tacttcaaag catcatataa atgaaatttt gagaaatgta catttaaata
      241 caaatgataa tattattgag attggttcag ggaaaggtca tttcacgttt gaattagcta
      301 aaaggtgtaa ttatgtaacc gccattgaaa tagatcctaa attatgtagg ataactaaaa
      361 acaaacttat tgaatatgag aacttccagg ttatcaataa agatatttta caatttaagt
      421 ttcctaaaaa taagtcatat aagatatatg gaaatatacc ctacaatata agtacagata
      481 taattcgaaa aattgttttt gaaagcacag ctacagaaag ttatttaata gtggaatatg
      541 gatttgctaa aaggttgcta aatacaaatc gttcactagc actattttta atgacagaag
      601 ttgatatatc catattaagt aaaatcccta gagaatactt tcatccaaaa cctagagtta
      661 atagctcgtt aattgtatta aaaagacacc cttcaaaaat atcactcaaa gatagaaaac
      721 aatatgaaaa ttttgttatg aaatgggtta acaaagaata cataaaacta ttttccaaaa
      781 accaatttta tcaagcctta aaatatgcaa gaattgacga tttaaacaat attagctttg
      841 aacaattctt gtctcttttc aatagctata aattatttaa tagataagtt aaggggtgca
      901 taaactgcat cccttaactt atcttatttt gcttaaacaa tcttggcccg taaactgcat
      961 aactcatatg taaatcgctc ctttttaggt ggcacaaatg tgaggcattt tcactatttc
     1021 cggcaattac ttccatgtaa agtataaccc actatacttt gcaagcaaag tgtgggctcg
     1081 ccgaggggtc ttgcagacgg ctctttcggc tcttttgagc cgagtatcag cgcaccctta
     1141 tggtgcaatt agctgatacc tgtttgacga tttgctgccc taccttttcg gtaggtatgc
     1201 aaacctcaaa ccaaaataaa aacccccttc gacttcgtca gggggttttt atttatataa
     1261 atctccgtaa ataatcgcga tttaataaca tcttcaattt tatttttttg cgtattttag
     1321 aaatgttttc actaaaaaat atattataca aaaaatacag taatcgatta taattctttg
     1381 ccagttaaat atcatatcgt tattaaaata tcggaaaaaa atatcaacaa caataaatgc
     1441 aaaagaagac cataaaaatt gtttattact catacatact ctccatttct atcattttta
     1501 aatttaccag tcatctctca aaaatctcct agctattttt cttttttaac cacataccgt
//
LOCUS       DANKEF010000181         1531 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000181 DANKEF010000000
VERSION     DANKEF010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1531
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.199"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1342
                     /locus_tag="PWZ48_003041"
     rRNA            <1..1342
                     /locus_tag="PWZ48_003041"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            1437..>1531
                     /gene="rrf"
                     /locus_tag="PWZ48_003042"
     rRNA            1437..>1531
                     /gene="rrf"
                     /locus_tag="PWZ48_003042"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          428 a          329 c          449 g          325 t
ORIGIN      
        1 gaaataatag taccgaagtt cctgatgtca cactgccgag aaaagcttct agtgagaaaa
       61 cagcggcccg taccgcaaac cgacacaggt agtcgaggag agaatcctaa ggtgagcgag
      121 agaactctcg ttaaggaact cggcaaaatg accccgtaac ttcgggagaa ggggtgctga
      181 tcatacgatc agccgcagtg aataggccca agcgactgtt tatcaaaaac acaggtctct
      241 gcaaaatcgt aagatgaagt ataggggctg acgcctgccc ggtgctggaa ggttaagagg
      301 agtgcttagc gcaagcgaag gtacgaattg aagccccagt aaacggcggc cgtaactata
      361 acggtcctaa ggtagcgaaa ttccttgtcg ggtaagttcc gacccgcacg aaaggcgtaa
      421 cgatttgggc actgtctcaa cgagagactc ggtgaaattt tagtacctgt gaagatgcag
      481 gttacccgcg acaggacgga aagaccccat ggagctttac tgtagtttga tattgagtgt
      541 ctgtaccgca tgtacaggat aggtaggagc cgtagaaatc ggaacgctag tttcgatgga
      601 ggcgctggtg ggatactacc cctgcgttat ggccactcta acccgcacca ctaatcgtgg
      661 tgggagacag tgtcagatgg gcagtttgac tggggcggtc gcctcctaaa aggtaacgga
      721 ggcgcccaaa ggttccctca gaatggttgg aaatcattcg aagagtgtaa aggcagaagg
      781 gagcttgact gcgagaccaa caagtcgagc agggacgaaa gtcgggctta gtgatccggt
      841 ggttccgcat ggaagggcca tcgctcaacg gataaaagct accctgggga taacaggctt
      901 atctccccca agagtccaca tcgacgggga ggtttggcac ctcgatgtcg gctcgtcgca
      961 tcctggggct gtagtcggtc ccaagggttg ggctgttcgc ccattaaagc ggcacgcgag
     1021 ctgggttcag aacgtcgtga gacagttcgg tccctatccg tcgcgggcgt tggaaatttg
     1081 agaggagctg tccttagtac gagaggaccg ggatggactt accgctggtg taccagttgt
     1141 tctgccaagg gcattgctgg gtagctatgt agggaaggga taaacgctga aagcatctaa
     1201 gtgtgaagcc cacctcaaga tgagatttcc catttcttta agaaagtaag atccctgaga
     1261 gatgatcagg tagataggtc aggagtggaa gtacagtgat gtatggagcg gactgatact
     1321 aatcgatcga ggacttaacc aataggttaa aagaaaagaa ctcggaaaaa aacttatgtg
     1381 acttcaaatc cagttttgag tgaacaaaat ttactcaata aaagataaca ccacagtgtg
     1441 gtggcgatag cgagaaggat acacctgtaa ccatgccgaa cacagaagtt aagcttctta
     1501 gcgccgattg tagtgagggg ttgccccttg t
//
LOCUS       DANKEF010000182         1526 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000182 DANKEF010000000
VERSION     DANKEF010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.200"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..>1526)
                     /locus_tag="PWZ48_003043"
     CDS             complement(<1..>1526)
                     /locus_tag="PWZ48_003043"
                     /inference="COORDINATES: protein motif:HMM:NF037291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VaFE repeat-containing surface-anchored protein"
                     /protein_id="HDL2262614.1"
                     /translation="VKGLVADDYYFLENAPLESFQPNTDKQAFTVAFKQNAPDDGETL
                     VVSVRNYLIPPKLETQASNKADGEKVVDPLETIVIKDSVSYTNLFVGKEYTISGVLMN
                     KATNAPILQDGKEITSTLAFIADKVNGTKDLEFVVNASVLKGQTIVVFEKLFRDGIEV
                     AAHADINDEAQSVKVNNPTVHTTAVFEDGLTVADPTGLFTLNDTVEITEILPGKAYLV
                     KGILMDKATGEPLLVHGNTVETSVGFIAKAENETVIVPFTFDLTGINGREIVVFESLF
                     RNDEELANHKDINDKGQTVRVTNPSVRTTATHKPTGLKEVNPLTKVTITDAVKYHDLV
                     VGKTYTVNGVLMDKVTEKPVLVDGKEVTSTLTFVAETTDGTVNLDFILDARALRGKEV
                     VVFEDLFREGVLLASHADITDNDQTIKISNPTITTKATNKNGGKEILALPNQTVLEWV
                     KVDGLAIGQIYRLIVQGYLTPDGTPFEGTHAEKVFAADKEMMEFLFEFLVDGRNLAGK
                     G"
BASE COUNT          380 a          337 c          289 g          520 t
ORIGIN      
        1 ccttttcctg caagattgcg accgtccact aagaattcaa ataagaattc catcatttct
       61 ttatcggccg caaagacttt ttccgcatga gtgccttcaa atggtgtgcc gtctggtgtt
      121 aagtagcctt gaacaattaa gcgatagatt tgaccaatcg caagaccgtc tactttgacc
      181 cattcaagca cagtttgatt tggcaaggcc aagatctctt ttccgccatt tttattcgtc
      241 gctttcgtcg taatggttgg attactgatt ttgatggttt ggtcattgtc ggtaatatct
      301 gcgtgagaag ccaataatac gccttctcta aacagatctt caaagaccac gacttctttg
      361 cctcgcaacg cacgtgcgtc tagaataaag tctagattga ctgtaccatc cgttgtttct
      421 gccacaaacg ttaaggtact tgtgacttct tttccatcta ctagcactgg tttttccgtt
      481 actttgtcca ttagaacacc gttgactgtg tatgtttttc caacgaccaa atcgtggtat
      541 ttcacagcgt ctgtaatggt gactttcgtt aatggattaa cctctttcaa gccagtgggt
      601 ttatgtgtag ccgttgtacg tacacttggg ttagttactc gtaccgtttg ccctttgtcg
      661 ttgatgtctt tatggtttgc tagttcttca tcatttctaa aaagactttc aaacacgacg
      721 atttcacgac catttatacc tgtcaaatca aaggtaaatg gtacgatcac cgtttcattt
      781 tctgctttgg caataaagcc aacactcgtt tctaccgtat ttccatgcac aagcaagggt
      841 tcaccggttg ctttatccat taagatccct ttgaccaagt atgcttttcc tggaagaatc
      901 tcggtaattt caacggtatc gtttaaggta aacaatcctg ttgggtctgc gactgtcaat
      961 ccgtcttcaa agacagccgt ggtatgaacg gttggattat tcactttcac actttgggct
     1021 tcatcgttga tatccgcatg tgctgcaact tcaatgccat cacggaaaag tttttcaaaa
     1081 acaacgatcg tttgaccttt cagaacacta gcattaacca caaattcaag gtcttttgtg
     1141 ccattgactt tgtctgcaat aaaggccaag gtacttgtga tttctttgcc atcttgtaaa
     1201 attggcgcat tcgttgcctt gttcatcaat actcctgaaa tggtgtattc ttttccgaca
     1261 aacaagttgg tataagaaac actgtcttta atgacgatcg tttctaatgg atcaaccact
     1321 ttttcaccat cggctttatt tgacgcttgt gtttctagtt ttggtggaat caagtaatta
     1381 cgtacagaaa ccactaacgt ttccccatca tctggcgcat tttgtttgaa tgcaaccgtg
     1441 aatgcttgtt tatctgtatt tggttggaaa ctttctaatg gggcattttc caagaagtaa
     1501 tagtcatcgg caactagacc tttcac
//
LOCUS       DANKEF010000183         1519 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000183 DANKEF010000000
VERSION     DANKEF010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1519)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.201"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1469
                     /locus_tag="PWZ48_003044"
     CDS             <1..1469
                     /locus_tag="PWZ48_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDL2262615.1"
                     /translation="LTTEIQLLNEKVNYLTNKLFGRSKETLFEETNGQLNLFSDEEIS
                     VSVPEAAATIIPVKGHQRVVGTKADKIKHLPITEKEHLLPLEEQFCEHCGSQMKDIGR
                     TKVREEIRFHQAMLDCLTHYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVA
                     ETIRMKFGQKVPAYRQENYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQ
                     EEILHGDETSYRVIESAKTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFT
                     GYLHCDGYSGYNAVESVRLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVL
                     EKKWKDFSPEKRLSCRQQELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEP
                     IFEDGRLELTNNRAERNIKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPV
                     KYLEFLFDKIPNLPVLSAETLDQLLPWNKDVQQHFSRN"
BASE COUNT          509 a          273 c          317 g          420 t
ORIGIN      
        1 atcttacaac tgaaatccaa ctattgaatg aaaaggtcaa ttatttaacc aacaaattat
       61 ttggacgctc taaagagact ctctttgagg aaacgaatgg gcaattgaat ctttttagtg
      121 atgaagaaat ttctgtaagt gttccagaag cagctgccac cattattcca gtaaaaggac
      181 atcaacgagt tgtaggaacg aaagcagaca aaatcaaaca cttacctata acagagaaag
      241 aacatctcct tcctttggaa gaacaatttt gtgaacattg cggttcacag atgaaagata
      301 tcggacggac aaaagtacga gaagaaattc gtttccatca ggctatgttg gattgtctca
      361 cccattatca acatacctat tgttgtaaaa gctgcgaaaa agaaggactt tcttctttta
      421 aaaaagcgat cgttcctaaa cctttgattt ctaatagctt aggttccaat agtttagtgg
      481 cggaaacgat ccggatgaaa tttggtcaga aagttcctgc ctatcggcaa gagaactatt
      541 ggaaacaaac acatgggttg gatatttcga gagataatat caccaattgg catattaaag
      601 cagtgcaaaa cgcgttagac cctttaggag aacgattaag agtctatctt aatcaagaag
      661 agattctaca cggggatgaa acaagttatc gtgtgattga aagtgccaaa acagatacct
      721 attactggca gttttgtaca ggaaaagaca gtcagcatcc catcgtttat tatcaccacg
      781 acgaaagtcg tgctggagat gttccgaaaa cttttctcaa ggagtttaca gggtatttac
      841 actgtgacgg ctatagcggg tataatgccg tggaaagtgt acgacttgtc tattgtttcg
      901 cgcatgttcg gcggaaattt ttcgaagcaa ttccaaaagg aaagaaaaac acggatattc
      961 ccgcagctca agcagtcaaa caattggata aatggtttgt cctagaaaag aaatggaaag
     1021 acttttctcc tgagaagcga ttaagctgtc ggcaacaaga actgcgtcca ttatttattg
     1081 ctttttatga gtggatggca acgatagatc ctgttgcaaa atccaagttg gatgcagccg
     1141 ttcaatatgc gtgtaaacta cgaagtggtt ttgaacctat ttttgaagat ggtcgtttag
     1201 aactcacaaa taatcgtgcg gagcgaaata tcaaagagct ggtgatagga cggaaaaatt
     1261 ggttgcattc cacaagtctt gaaggagcac gaacttcagg aatcattctg agtgtatata
     1321 aaacagcaga acttaacgga ttgaatcctg taaagtatct agagtttcta tttgataaga
     1381 taccaaatct tcccgtactt tcagcagaaa ctttagatca actgttgcct tggaacaagg
     1441 atgtacaaca acatttttct cgtaactaaa aacgccaaac attcccgatt ctattctata
     1501 ggtcgggtta tttgacgta
//
LOCUS       DANKEF010000184         1517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000184 DANKEF010000000
VERSION     DANKEF010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.202"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            289..696
                     /locus_tag="PWZ48_003045"
     CDS             289..696
                     /locus_tag="PWZ48_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262616.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            710..1111
                     /locus_tag="PWZ48_003046"
     CDS             710..1111
                     /locus_tag="PWZ48_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262617.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1113..1484
                     /locus_tag="PWZ48_003047"
     CDS             1113..1484
                     /locus_tag="PWZ48_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262618.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          575 a          185 c          264 g          493 t
ORIGIN      
        1 tttgagggga agtacatata gttgttgata tagttgctaa tattgttagc taatttgtta
       61 gtaaaactgt atacgaattt agatagtaaa aaaacagttt gttccttata tagaggcact
      121 tttactactg attaaatgtc agatatgctt acaaatatac ttataaatat gcttacaaaa
      181 ttagctaata tttccgttga caaagctttg ttactattgt taaactaaat tgactagcaa
      241 aagtgaaaca taaatttatt aaataaatgt aaaaattagg agaaatcaat gtcttataga
      301 gtacaattaa taatttcaga agatgtagaa agtcagcaat ttggcactaa tgttattaat
      361 aaagtaatta atccagctct ttctattaat gcgcctttaa ttcctactgc tttatcattt
      421 tctgtaacag ctgtggtgtc tgagatagag gatacagaaa agataaaaat agtagaaatt
      481 gaagttttaa acaagaatga aaaacaaatt ttttctacgg gtgaagtatc ggtaagcttg
      541 cctcctcaag ttaacgatat aaactttaat ataaacgcca gaaatgtctt ggttgaagaa
      601 gcaggagagc attatgctgt tgttaaattt aatggaactg agattggaag gcaaatcttt
      661 gatatcaagg tcaacaaacc agtggaaaaa aattaaggag acgatacgga tgcttgatat
      721 agtaccatca aatacacatc ggagtggtag cttaataaat tttgctgcga tttttgcttg
      781 cgcatcggta attctagcat ctccagttgt tgtggatgca agttctactc cgaaaactaa
      841 aaatgataat caacttgtaa ttactacaaa aaatgagatt aacacaattt caagtaatga
      901 tggcaaaatt tttgatgtga taaatacggt tttaaaaaga aaagctgaaa tcaatataaa
      961 atatgatgaa gatttaaact tgtatttctt tgttatcaaa acaacatctg aattgttttc
     1021 ctcagattac gatgttttgg atactctcga taatgtatta tctgactata aatatatggg
     1081 gaaatctgta gtagcaactt tgggggaata aaatgtttga ctttaatgaa tatttatcta
     1141 tttccaaaca aattgaatca gatgataaat ataattctaa agaatcttat agaagaactg
     1201 ctgtttcaag agcatactac agtgctttta aaaaatctga tgagtatttg aaagaaaatt
     1261 atgatattat ttataatgga agtagcggaa aaggaagcca ccaaaccgtt tggaatttgt
     1321 tttcaacagt taaggaattg aataccttag gaatacataa tagtgggtat agaatgttag
     1381 agaaaagaaa atgtgctgac tatattccta atgaaaccat tacgaaaaca gatatggcgc
     1441 tgatgaatcg agaagcagaa aaaataataa acaaactcac ctaatgaatt tcaagcgagt
     1501 ttgtttgtta ttttttt
//
LOCUS       DANKEF010000185         1455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.203, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000185 DANKEF010000000
VERSION     DANKEF010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.203"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            108..1040
                     /locus_tag="PWZ48_003048"
     CDS             108..1040
                     /locus_tag="PWZ48_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HDL2262619.1"
                     /translation="MLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            1112..>1455
                     /locus_tag="PWZ48_003049"
     CDS             1112..>1455
                     /locus_tag="PWZ48_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262620.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQ"
BASE COUNT          640 a          169 c          227 g          419 t
ORIGIN      
        1 atgaaataaa tcaaaagtat gatgaaacat taaaaaatgt ctattcttca cttatcgttt
       61 caaatataga agaaacttca tcttctgaaa taaaaaataa gctttctttg ttaaaattaa
      121 aagaaaagga atatattatt gacgatgctg tttctgtccc tcttatttta aataaacgag
      181 agaaaaataa tttaaagaaa aaaatgctca ttccatttga ttcagaagtg aaaggattac
      241 tcaataaact aactatttct aatatagact cgactaggga aaaaatcata aaaatatttg
      301 ataaaattaa taatttattc aagaatgaca atgctgaatt aaggaagggt cttttagagt
      361 ttaaacttca agaactagaa cttcattact cttatttagt gaaaattacc gaagaaaaag
      421 aacaacaaaa agcaataaaa gaacagatga tagaagagga aaaagttcgt agagaaattg
      481 atcgtgaaaa gaaacgtatt gataaagaac aacgtcaatt caattctgaa atttccaaat
      541 taatcgcata tatgcaaaaa gctaatgcag atgtggaaaa agaattatat gcaaataaaa
      601 ttcaagaact ggaagaaaaa ttaaaagaat tagaagttat acgtgaaaat gtattacaaa
      661 gagaattaaa tactagagcc ggttatgtgt atgtaattag taatattggg tcttttggtg
      721 aagacatata taaaattggt atgactagaa gattagaacc tatggataga ataaaagaat
      781 tgagtagtgc atcggttcca tttgaatttg acgttcatgc gatgattttt tcagaggacg
      841 ctccgtcttt ggaaactaaa ttgcataatc attttagaaa acaagaagtt aataaaatta
      901 atcaaagaaa agaatttttc aaagtttcac tagacgaaat agagaaagtt gttttagaaa
      961 attataatgg aacagtaaca tttacaaaat tagctaaagc tgaacaatat agacgatctt
     1021 tagaattgtc aaaagattaa atatataaaa agccttcggg ctttttcttt taacaattaa
     1081 taggaacgta tattcgaaag gaatagaaat aatgtggtta gaaaaaataa atgacaaaaa
     1141 atttaagtac accgaacgtt atacggatcc cttaactgaa aaaaagagaa aagtgtccgt
     1201 tactttatct agcaattcac gccaagcttg gaatcaagct aacttacttt tgaatgaaaa
     1261 aatcgcagaa aaaataaaac gaaatgaaga attaccacta acttttggag aattaaaaac
     1321 taaatgggat gaaaaataca agccaactgt aaaagaaagt tcctatcgaa caactcaggt
     1381 ttatctatca ttaatttcaa aatatataaa agatgatgta cttgtaaaaa acgttaatag
     1441 taatcttata caaga
//
LOCUS       DANKEF010000186         1455 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000186 DANKEF010000000
VERSION     DANKEF010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.204"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            97..>1455
                     /locus_tag="PWZ48_003050"
     CDS             97..>1455
                     /locus_tag="PWZ48_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HDL2262621.1"
                     /translation="MNHKNSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIK
                     IMEEPAGYSKEKINGIMKKYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLT
                     VPTDPIPYIKSIITEGTGGLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLV
                     ERQKISPNITDLFDEKIAIHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQ
                     SPRISDDSAYKETLKALQSNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNR
                     RKELQTYMQGLHYTDTAVKKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQH
                     QTDYFIYNNKYSREQAATLNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPAL
                     TIDPTESLSNRFNGKQVFVTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSAT
                     WAT"
BASE COUNT          524 a          270 c          254 g          407 t
ORIGIN      
        1 cgtagactac aaaaacagta ttccttgaag attcattgga aacgtcgatt tatcagtatc
       61 gctgtattat taggatgttt ttcgggtgtc tttttcatta atcacaaaaa ttcgccttct
      121 ttcttgctgt ttaatttatt taaagtaaac aagactttct ttaaccagca aggcgccgta
      181 aaagataacg gaccagtcat tcaattccta aacaatatcg acattaaaat catggaagaa
      241 cctgctggct attcgaaaga aaaaattaat ggaataatga aaaagtacaa cacaaaggca
      301 gatgaaatca accaaacaag aagtgactgg ttaaacaatc agacgatcgt tctcaattta
      361 agtgaaagct tcagtgatcc tgcacgtgtc cctaatctga cagtacctac agaccctatt
      421 ccttacatca aaagcattat aacagaagga acaggaggat tgatgttatc cgtcggttat
      481 ggtggcggaa cagctaacat ggaatgggaa ggactgacag gcttgagtat cagtaacctg
      541 tctgcttcgt tagttacacc ttatacccag ctagtggagc gtcagaaaat aagtccaaat
      601 attaccgatc tattcgatga aaaaatagcc atccatcctt ttacagctgc tctttatcgc
      661 agaaaacaag tgtttgataa atttggtttt gacaggttct attatttaga cagtccggat
      721 aaattaactt atacggataa aatagaacaa agcccacgca tatcggatga ttcagcatac
      781 aaagaaacct tgaaagcttt acaaagcaat acagaaacgt cacaattcat ccaattatcc
      841 actatgcaaa accatatgcc ttatgatgat tactatagtg aattgaacta cactgctgaa
      901 ggaactgctg tcattcctaa tagaagaaaa gaattacaaa cttatatgca aggccttcac
      961 tataccgata cagcagtaaa aaaattcatc caagaaatcg acaaaatcga taaaccgatt
     1021 acctttgttt tttatggtga ccatttaccc gcaatctata gtgggaatga ttcgaaaaaa
     1081 tatggattag aacagcacca gacagattat ttcatttata acaacaagta tagtcgtgaa
     1141 caagcagcta cgttaaataa agaagttgtt gctcctaata actttggtgc tatggctctt
     1201 gagcatgcga atatcaaagt aactccttac tatgcgttgt tgacagaagt atctaatcat
     1261 ctacctgcat taacaattga tcctacagaa agcttgtcta atcgttttaa tggaaaacag
     1321 gtctttgtta cagaggatag tcaaattatc caagaaaaaa atttaactga gaaacaaaaa
     1381 gaaattctag aagattatcg tctgatacaa tatgatcttg ttgcaggtaa ccagtacagt
     1441 gcaacatggg cgaca
//
LOCUS       DANKEF010000187         1424 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000187 DANKEF010000000
VERSION     DANKEF010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.205"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(150..1337)
                     /locus_tag="PWZ48_003051"
     CDS             complement(150..1337)
                     /locus_tag="PWZ48_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HDL2262622.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DANKEF010000188         1416 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000188 DANKEF010000000
VERSION     DANKEF010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1416
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.206"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            30..>1416
                     /locus_tag="PWZ48_003052"
     CDS             30..>1416
                     /locus_tag="PWZ48_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HDL2262623.1"
                     /translation="MFAALGIGIGAGYFAYLVEDTQLPTKKELQTELGNITETSKIVY
                     ADNTEISKIQTDLMRTTISSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEA
                     TGIGSSGGSTLTQQLVKQQILTDETTFKRKANEIVLSAQVEKHFSKDEIITTYLNVSP
                     FGRNNKGQNIAGVQEAARGIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKED
                     LSAGLARKDFVLFSMYREGQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMN
                     EATRIIAQQLAEKDNADMSDSSVSEAYYQKAQQTIQNKGYTIHSTIDKDIYAALQTGV
                     ENYGYMLDDGAGSQVETGNVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIK
                     PVLVYGPAIDMGLVGSESRVSDYATTWQEGKNAGEKIVNATNEGSNTFQTVRESLEWS
                     NNIPAYHLYQDV"
BASE COUNT          468 a          283 c          302 g          363 t
ORIGIN      
        1 tgactgtttt tattactgtt cttcttttgt tgttcgcagc tctaggtatc ggaataggtg
       61 ctggctactt tgcctatctt gtcgaagaca cacaattgcc gactaaaaaa gaactgcaga
      121 cagaactagg aaacattacg gaaacgtcaa agattgttta tgcagataat acagagatat
      181 caaaaatcca gacagatctg atgcggacga ctatttcatc tgataagatt tcgcctttat
      241 tgaaaaccgc gattatcagt acagaagatg aatacttcga taaacaccaa ggttatgtcc
      301 ctaaagctgt tttacgagca ctagtttcag aagcgactgg catcggctcg tctggaggat
      361 ccactttgac acagcaatta gtcaaacaac agatcctaac cgatgaaaca acattcaaac
      421 gtaaagcaaa cgagatcgtt ctttctgcac aagtagaaaa acacttttca aaagatgaga
      481 tcattactac gtatttgaat gtctcaccat ttggtcggaa taataagggg caaaatattg
      541 ctggagtcca ggaagctgct agaggaatct ttggtgtaga tgcaaaggat gtgactctgc
      601 cgcaagctgc ttatatcgct ggtttgcctc aaagccctat cacgtatagt ccatacacga
      661 atacaggcgc gctaaaagaa gatctttctg ccggtcttgc cagaaaagat tttgttttat
      721 tcagtatgta ccgtgaagga caaattacga aagaacaata cgaagaagcg aaagcctatg
      781 atcttacaaa agactttctc ccacaacaaa tcgcggaaca gactgacaga gaattcttgt
      841 attataccgt gatgaacgaa gcaacaagga ttattgcaca acaattggca gaaaaagata
      901 atgctgatat gtcagattca tcggtcagtg aagcttatta tcaaaaagca cagcaaacga
      961 tccaaaacaa aggatatacg atccattcga cgattgataa agatatctat gctgcattgc
     1021 aaaccggcgt agaaaattat ggctacatgt tagatgatgg tgctggtagc caagtggaga
     1081 ctggaaatgt attgatggat aacagaaccg gacgtatcta tggcttcgtg ggtggtcgga
     1141 attacagcca gaatcaaaat aaccacgcct ttgatacaga acgtcaagct ggttcatcca
     1201 tcaaaccggt tcttgtttac ggtcctgcaa tcgatatggg acttgtcgga tcagaatccc
     1261 gtgtatcaga ttatgcaacg acttggcaag aaggaaaaaa tgctggtgag aaaatcgtca
     1321 atgcgaccaa tgaagggtcg aacaccttcc aaaccgtccg tgagtcactg gaatggtcca
     1381 acaatatccc agcttatcac ttatatcaag atgtgt
//
LOCUS       DANKEF010000189         1377 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000189 DANKEF010000000
VERSION     DANKEF010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.208"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            44..1339
                     /locus_tag="PWZ48_003053"
     CDS             44..1339
                     /locus_tag="PWZ48_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HDL2262624.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     LYQDILYAVKKRDQSLFEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGP
                     LECLNNHIKVLKRNAYGFRNFYNFKLRIMIRHGKTFLTK"
BASE COUNT          427 a          274 c          263 g          413 t
ORIGIN      
        1 cctttagaat taatttggct accaattcat aggaggattc aagatgtctc atactcagct
       61 tatcaaagat accctaaata ttctagacct aaatatctat ttcgaggaaa attgtttaac
      121 taaagaaaaa tacaaagggc aaatttgctt gatttacaaa ggaactcttc tgtacaagcc
      181 agaagagtgt tcccattgtc tttgtgtcgt cccctctagg atcattcgtt gggggacgac
      241 aaccgttcgg ttgttactga atgatgtgtc tgagtatcgc acttatctgg agctgaagaa
      301 acaacgattt aaatgtaagt cttgccaaag aacattcgta gcggatacct ctgttgctga
      361 aaaacactgt ttcattagtc aaaaagttcg ttggtctgtc attgctcgct taaaagagaa
      421 tacttccatg acggaaatcg ctcgtcagaa aaatatttcc acttcttcgg tctatcgtgt
      481 catgaagcga ttctaccgcc cattgaatcc tttcaaacag actttgccaa aggtcttatg
      541 ctttgatgaa ttcaaatcgg ttcgaagtgt tgccagtgca atgagtttta tcatgatgga
      601 cggacaaagc catgcattat tggatattgt cgaaaatcga cgcttgcctt atttagaacg
      661 ctatttttct cggttttcat tagcgactcg tgaagccgtt caattgattg ttattgatat
      721 gtatgcgcct tatgtttcgt tggtgaaaaa gcttttcccg aatgctcagt tgatcatcga
      781 tcgtttccac atcgtccaac atattggcag aactttcttg aatcatcgag tgaaagaaac
      841 cacggtacga cttaaaggaa attcccactc tcaacgaggg ttagggaaaa aattgaagcg
      901 ctattggaaa ctcctacaaa aagaagaagc aaagttaaac tatgaaaaac gggtttggcg
      961 tgccagtttt aaagattatt tgactgaaag tgagattgcc gatcgactgt tagctgcttg
     1021 tcccaactta cgccaaggct atcagttata ccaagacatt ctttatgcgg taaagaaaag
     1081 agatcagtca ctatttgagg attgtttaac tagagacatt agcggtctgc cagaaaccta
     1141 tacaaccact ttacgaacgt ttaaaaagtt tctgccacag atccaaaatg cgcttcacta
     1201 cagctattca aacggaccat tggaatgctt aaacaaccac atcaaagtat taaaacgcaa
     1261 tgcgtacggc tttcgcaact tttacaattt caaactacga attatgattc gtcatggcaa
     1321 aacattttta accaaataaa cagaacagaa agaggttttc ttctttctgt tctgtcc
//
LOCUS       DANKEF010000190         1373 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000190 DANKEF010000000
VERSION     DANKEF010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1373
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.209"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            191..445
                     /locus_tag="PWZ48_003054"
     CDS             191..445
                     /locus_tag="PWZ48_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDL2262625.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="PWZ48_003055"
     CDS             446..763
                     /locus_tag="PWZ48_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDL2262626.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          492 a          196 c          268 g          417 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaaggcaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accgtaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat ata
//
LOCUS       DANKEF010000191         1364 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000191 DANKEF010000000
VERSION     DANKEF010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.210"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            106..1185
                     /locus_tag="PWZ48_003056"
     CDS             106..1185
                     /locus_tag="PWZ48_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2262627.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
BASE COUNT          370 a          361 c          372 g          261 t
ORIGIN      
        1 gcggatttta taattaactt agccatttgg aaataaaaaa acgccacgtg aattccatgt
       61 tatattgaag ttaccacaac tctcaataga caaggatgaa ttcacatgac gcaaaaccat
      121 gataacacag tttcacgcaa gggaaaacac ctaacctact cagaacgttg ccaaatcgcc
      181 atcctgaaaa aggaaaacta ttccaaccgt caggttgcgg atgtcctggg ctgcgtgccg
      241 cagaccataa acaacgaaat caaacgcggg accgttactc agcttaaccg ccagaatcag
      301 aacgggaagg tctacgacta ttatcatgac gtctacgacg ccgatgccgg gcaggcggcc
      361 tacgagaaga accgcttgaa ctgcggcaga cggcccttat gggccgacac ggacgccttc
      421 attgaatggg cggacgacat gatgctgttg gaaaaatggt ccccggacgt ggtggtgggc
      481 tttgccagaa aacacgaatt gttcgacccg gccattatcc ctgggaccac caccctctat
      541 cagtggatcg accgtggcat catgaaaacg aagaatatgg acctgctaga aaaactgtcc
      601 cgcaaaccga aatcgtcccg tcatggcaac cgcccgaata aacgggtgct agggaaatcc
      661 attgaagagc gcccggaggc ggttggtacc cggaaaacct tcggccattg ggaagtcgac
      721 acggtcgtag gaaacaagac caagtcggat gccgtgctgc tgacgttggt ggaacggcag
      781 acacgcttcg aggtcattct gaagctggcc gggaaggatg ccgaatcggt caaccaagcg
      841 gtccgacagc tgagagaacg ggctggtgcg tacttcggcc agctgttcaa gacggttacc
      901 tcggacaacg gctcggagtt cgcgggactg cacgatgcct tgcaggatgt ggcaaacatc
      961 tacttcagcc acccctatgc ttcctgggag cgcgggacga gcgaaaacca acacaagctg
     1021 attcgccgtt tcatccccaa gggcagccca atcggcaacg tgagcgaaaa ccaaatcttg
     1081 cggattcagc gttggatgaa cgattacccg cgaaagattt tgggctatgc aacgccgcat
     1141 gacacgttcg tccaggcatt taggcaggaa cggttgacgg cttgacacgt gggcccttga
     1201 caacgagcct ccctcggcat ggcttgtgtc tggaaagctg gccggggcca gccagtcggg
     1261 agactaccat gcctcagctc attgtcaagg gcccactcga ttagaaaaat tagacggttg
     1321 gtggtaacga gcaaccgggt caccatgggt ggctaactta aact
//
LOCUS       DANKEF010000192         1353 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000192 DANKEF010000000
VERSION     DANKEF010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1353
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.211"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            76..>1353
                     /locus_tag="PWZ48_003057"
     CDS             76..>1353
                     /locus_tag="PWZ48_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HDL2262628.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQ"
BASE COUNT          461 a          262 c          257 g          373 t
ORIGIN      
        1 ggtattaaaa aacatatcgt tctctagtat gattaaatca ccacaaaata aacaactgag
       61 aggacgatac gctatatgaa tgattctatc aaaaaaatgc tgagaataac agagaaagat
      121 ttgatgatta cagaggtctc ttacgagacc cttcagaaga aaaagacgtt ggtcgtcgat
      181 gctgttctct cgcctactcc tcgtgcttgt agaagttgtg gttctactgt ggtagatgga
      241 aacgggaaag caattatagt gaaaaatgga aaaaaggaaa cgattgtccg ttttgaacaa
      301 tacaatcata tgcctttggt tatgcgccta aaaaagcaac gctatacctg taaaaactgt
      361 cgcacccatt ggacggctca aagttatttt gtccaaccca gacattcaat cgcaaatcat
      421 gttagatata aaattgcttc tttactgact gaaaaagtat ctttatcttt tattgcgaaa
      481 agctgtcagg tatctttgac caccgttatt cgtacattga aagagtttaa aagctattta
      541 ccaaagcaat ctaagaagat cctccccaga gtattgatgg ttgacgaatt tcgttcgcat
      601 gcttccatag aagataagat gagctttatt tgcgcggatg gcgaaacagg aaaattaata
      661 gatgttttgc ctacgcgtaa attacctcga ttaacaagct atttcttagg ttgtaccaat
      721 ccagaagaag tagaattctt ggtgacagac atgaacgccg cctacttcca gctcaccaaa
      781 cgtgttctgc caaatgcgaa agtcgtgatt gatcggtttc atattgtcaa acacatgaat
      841 caagcgttca atgagttgcg tatccgtgaa atgaatgaac ttcgtaaagc cggacagaaa
      901 agccaggcag aaaaactgaa aaagaactgg cgctttttgc taaaaaatcg tgcaaacatc
      961 aaccattatg aatacaaaac atggaaaagt ttccgagcac caaaataccc atttcttact
     1021 gaagcaatga tgattgatcg attgcttgag ttttctccgc ccctaaagga ggcgtatccc
     1081 ttttttcatg aattagttga agcctttcga gacaaagacc ctgacttatt tttctcctta
     1141 ttggcagaac ttcccgaaac gttagatgac agctttcggg aaaagcttca aaaccttctg
     1201 acctatgaag aaggcatcac caacgcaatg atctatcctt attccaatgg aaaaatagaa
     1261 gcgaagaata cccacataaa gacaatgaaa cgagtatcct acggatttaa atcatttgag
     1321 aacatgagaa ttagaatctt tttgatcaat caa
//
LOCUS       DANKEF010000193         1349 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.212, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000193 DANKEF010000000
VERSION     DANKEF010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.212"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(51..1223)
                     /locus_tag="PWZ48_003058"
     CDS             complement(51..1223)
                     /locus_tag="PWZ48_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HDL2262629.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIGNSHNRL
                     KSTNLLERLNQEVRRREKIIRIFPNRTSANRLIGAVLMDLHDEWLSSTRKYIKFDQ"
BASE COUNT          371 a          279 c          256 g          443 t
ORIGIN      
        1 gtcaagtcca taatcctgtg taaaatacta tacaatgttt taccggtctc tcattgatca
       61 aacttaatat attttcttgt agaactgagc cattcgtcat gaaggtccat aaggacagct
      121 ccaattaatc gattggcaga cgttcggttg ggaaaaatcc gaataatctt ttctcttctg
      181 cggacttcct ggttcagtcg ttcaagaagg ttggtgcttt ttagccgatt atgactattt
      241 ccgataaccg tgtattgaaa ggcatcttcg aagccattat ccaatatttc gcaggctttt
      301 gtatatttag gctggtcgat atattcaccg actaaggcat tcttagctgt tcgagcgagt
      361 tcaatatccg taaacttaaa gatcgctttt actgcttctc taaacggttt tgaattcttt
      421 tttggaatgg aactgaagat gtttcttaaa aaatggacct ggcatctctg ccaacttgcg
      481 ttggtaaatg acttacgaat cgcagacact aggcctttat gggcatcaga aatgatgagt
      541 tctgtcccct ttaggccgcg ttcttttaag tattcaaaga agatggacca tgtgtcatca
      601 ctttcttcat tttgaatcat gaagccaatg atttcacggt cgccaccttc tgttatcccg
      661 atcgcaatat ggcagctttt agaaagcact cgatggtcct cacggacttt tatgtagaga
      721 acatcagtca tcagataagg ataattcgta cctgagagtg aacggttctg ccattcgttg
      781 accatcgggt ctaactgctc agtcaggcta gaaacaaaag atttcgatac agattttcca
      841 catagctctt caacaatctt tgaaacttta cgtgtcgaaa cgcccgaaac atacatctca
      901 agcattgaag cgagcagtgc cttttcattt cgctgataac gctcaaacac cgtcggtgaa
      961 aattcaccat cacgtgttct aggcactttt aattcgagtg tacccacacg agtcgtaaag
     1021 tctcgctcat aatagccatt tctttgactc tgacgacttt ctgaacgttc atagtcatcg
     1081 gcttgaatat attctgttcg ttgattttcc atcaattggt tgaaaacagt ggttaaaata
     1141 tttttagaaa catcatcttt taccgaatgt tcaataatac tttgaacctc ttcgttgttc
     1201 agtgtaaaat gtacttgggt catgtaacgt cctcctgggt atgtttttgt ggttaaaaac
     1261 attgtaccgt aaaagggctg ttacatggcc ttttatcttt tacacaatta tatggacttt
     1321 atcgaaacag actaaaaaca gtgattacg
//
LOCUS       DANKEF010000194         1334 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000194 DANKEF010000000
VERSION     DANKEF010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.213"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            164..715
                     /locus_tag="PWZ48_003059"
     CDS             164..715
                     /locus_tag="PWZ48_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF334 domain-containing protein"
                     /protein_id="HDL2262630.1"
                     /translation="MKRLEASTSNFNNKLDVFEEKTKHARLNFETTAKHYIKRLDEDN
                     LKLDFQQAIQDELSDTKDEIREVTKQAREETKEYKEILESKIKDHNKVVDKSNTALKV
                     MTKGVTNIFFVLIIFVLVMLVTGPIGHFFGIEHLYSFINGFIDDHESAWRYLMLILYA
                     VPYVFFAIILWLIALFFRVFNDL"
     gene            1010..1183
                     /locus_tag="PWZ48_003060"
     CDS             1010..1183
                     /locus_tag="PWZ48_003060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262631.1"
                     /translation="MSEKTKKIIRVSLAFSQVVVQSLIVYYTYQQYKWFCCKVFQKIY
                     FSVKLRKKTERTE"
     gene            1183..>1334
                     /locus_tag="PWZ48_003061"
     CDS             1183..>1334
                     /locus_tag="PWZ48_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2262632.1"
                     /translation="MNHFKGKQFKKDVIIVAVGYYLRYNLSYREVQELLYDRGINVCH
                     TTIYRWV"
BASE COUNT          493 a          187 c          258 g          396 t
ORIGIN      
        1 aaagaaggca ctctcttcgg gttttcggtc tgtactgaaa tcaaggtatt attgggaatc
       61 ccagcttaaa tcatagatac cgtaagggat tttattcttt atttaaaact ttgcaacaga
      121 accgaagagc gagcaagaca ggaacgagag aacaaacata ctcatgaaaa gactagaggc
      181 ttcgacctcg aactttaata ataagttaga tgtttttgaa gaaaagacta agcatgcaag
      241 attaaacttt gaaacaactg caaagcacta tattaaacga cttgatgaag ataatttgaa
      301 attagatttt caacaagcta ttcaagatga attaagcgat actaaagacg aaatacgtga
      361 agtgacgaaa caagcgagag aagaaacaaa agagtataaa gaaatcttag aaagtaaaat
      421 aaaagaccat aacaaagtgg ttgataaatc gaatacagcg ctaaaagtga tgacaaaagg
      481 cgttacaaat atattttttg tgctaattat ttttgtactt gtaatgcttg tgacaggtcc
      541 gataggtcat ttctttggta ttgaacattt atatagcttt attaatggtt ttattgatga
      601 ccacgaaagt gcatggcgat atttgatgtt gatactttat gcagtaccct atgtattctt
      661 cgctatcatt ctgtggctca tagcattgtt ttttagagta tttaatgacc tttaggtaga
      721 tttatatata aaaagggcta aatacgcaaa ataccccctt aaaatgcaaa ataaatgcaa
      781 ataagggggt aactccttat gaaaaaacaa ctaatagtat tataacacgt ttttatgccg
      841 agaaaactta ttggttggaa tgggctatgt gttagctaac ttgttagcga gttggttgga
      901 cttgaattgg gattaatccc aagaaagtac caactcaaca acacataaag ccctgtaggt
      961 tccgaccaat aaggaaattg gaataaagca acgaaaggag ttgagtaaaa tgagtgagaa
     1021 aacgaagaaa atcattcgag tatcattagc tttttcacaa gtagtagtgc aatcattgat
     1081 agtttattat acttaccagc aatataaatg gttctgttgc aaagttttcc aaaaaatcta
     1141 ttttagtgta aaattgagaa aaaagacaga gaggacagag taatgaatca ttttaaaggc
     1201 aaacaattca aaaaagacgt cattattgtc gctgttggtt actacctgcg ttacaatcta
     1261 agctatcgtg aagttcagga attgttatat gatcgtggaa taaatgtttg tcatactacg
     1321 atttatcgtt gggt
//
LOCUS       DANKEF010000195         1325 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000195 DANKEF010000000
VERSION     DANKEF010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.214"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(315..398)
                     /locus_tag="PWZ48_003062"
     tRNA            complement(315..398)
                     /locus_tag="PWZ48_003062"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(362..364),aa:Leu,seq:cag)
     gene            926..>1325
                     /locus_tag="PWZ48_003063"
     CDS             926..>1325
                     /locus_tag="PWZ48_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2262633.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSV"
BASE COUNT          402 a          235 c          207 g          481 t
ORIGIN      
        1 tttgaacgtt taaacacatt caaaatatag taagaggtca tgcgatttat tctttcgcat
       61 gacctctttt tgagtttaag tttagtcttt attccttaac tatcgtttgc cagtatttct
      121 tgtatcagaa tctataaatc tactatttga atcaatctat taggttttaa actgtcccac
      181 cttcacacaa attttttttg atttatttga atctggttcc gttgtttaaa atttttccta
      241 tattctttta gataatatgt tattttcttt ctcatcgctc caggtgtacc ttctctttat
      301 aaaaaaacaa attatgcggc taagaagact cgaacttcca cgaggtcacc ctcactagcg
      361 cctgaagcta gcgcgtctgc caattccgcc atagccgcat cacatctttt agtttaggat
      421 tacatgtgtt ttttgtcaag aacatatttt tgttcttttt catttctgta cgccctccct
      481 tatgccatct acacaaataa ttcatccgag caactccatc atctattgag ggttggtgaa
      541 aaaatctgtt ttatccatat gatcgatttc aatgttaaac gaacgactct gggattagta
      601 actattttat ttttttcttc atttcttttt tcctcttttt ctcgattcag ctatcttctt
      661 tcatttggat attgattatt tcttctgttg attactatcc ttctggaatt ctagacgctg
      721 aagtaattca cgttttcttt cttctagaga tcataagtaa cgaatgaacg aacttcaaat
      781 aattttttta ttgtaattca tatttgtatt gttcgatgat tcttaatacg atatgacaca
      841 ataaataaaa ataggcatgg ctatttacaa caatagtgct atagtagaaa acaaatgaat
      901 aaggttactt aaagaggtga atacgatgaa actgatcaaa ctatgggaag cagatttaaa
      961 gaaagcttat cagctacaaa ctagctttgg accaaatgag agtggattta tcaacaatgc
     1021 ttatggctat acatttgctg aatttttaga ttatgtaaaa aaatgcgaaa aaacgtcttt
     1081 aggaatcgat cttcctgaga gttacgttcc cgcaaccgtc tttattttag aaagtgatga
     1141 acatgagtat gtaggaattt ttaatttgcg ccataaactg aacgaaacat taagaaacgg
     1201 cgctggccat attggttacg gcatctcccc taattatcgt aaaaaaggat atgccacaca
     1261 aggattagca ttagtactac aagaggcaaa aaaaatcggc attgatgagg cacttttgtc
     1321 tgtaa
//
LOCUS       DANKEF010000196         1317 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000196 DANKEF010000000
VERSION     DANKEF010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1317
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.215"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(370..443)
                     /locus_tag="PWZ48_003064"
     tRNA            complement(370..443)
                     /locus_tag="PWZ48_003064"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(407..409),aa:Ile,seq:cat)
     gene            complement(998..1312)
                     /locus_tag="PWZ48_003065"
     CDS             complement(998..1312)
                     /locus_tag="PWZ48_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262634.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          486 a          208 c          195 g          428 t
ORIGIN      
        1 taattaacta ccactccaat tataagtctt tttcccatta ttttttacag ttatatggta
       61 tgctttttaa tggcttcaaa tataaaagag tttaaagcgt aacacactta tggggaagtg
      121 gtttttgggg aacgctttaa actcttcttt attattatct cacaatataa cccaaatgtc
      181 tttccattta aaaatcaaag taaaactttt caaatataca gaagtataac tatgtgaaac
      241 atcctttcat taatccataa aaggatacat aaaaaagcca ctcatttgag tggcaatgaa
      301 gaaaagcttt agcttgtata atactcttca aaaaattcta acacagaacg attcaattgg
      361 ctacattaat gtaccctgta ggactcgaac ctacgaccgg acggttatga gccgtctgct
      421 ctgaccaact gagctaaggg tagtggttgt tgccacataa agccataaac aatcaaccag
      481 tagaatgcgt ggcaacaaac ctgttatcgc atatcttgga gtgtgactat ttatgagtga
      541 tagtgaagat atgcgtctcc atatatgtat ggttgagaca gataaacatg tatgaaaaac
      601 gttgttaaat caacgttttt ttattttggt caaggagaga aaagtagaga aatacaaaat
      661 tttttgcatt gattttgcat tcaaaaaaac attcaaacat ttagaaatcc ccaatacgta
      721 ttcaggaaaa tctattttaa ggtttacaaa aagaactttt gttcgtacac tctttttgag
      781 gtgaactttt atgctgatgg aaggaaaaac acaattatgg tttaagcatg ttccttcaaa
      841 taagtttttt aattataaac tctagagaaa ttaattcatc ctaacataaa ataattgttc
      901 actcaaaagt ttttctagaa caattaaaac agcaggcttc acattcgcta tacatttgta
      961 agaagtaata attcctgtac taaaaggcat aattgcttta attatttttt gacttctctt
     1021 caaacacaag aagtagttgt gtaataattg cagaaataag gattccaata atagatataa
     1081 aatttgacat ggaaacgtct ataacattaa aagaataaag tgttgaagaa agtaaactga
     1141 ttgaaataac ccctccgtaa aaaatagtct gtgaatatac ccactttttt tcagaagacg
     1201 tacttcgttt ggttctaaaa cctatccctg catttctact cttatcaaaa aaccaaatat
     1261 ttagaagtaa taatattgct attattaaga taaaattact gtaaaaatcc aaaagct
//
LOCUS       DANKEF010000197         1313 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000197 DANKEF010000000
VERSION     DANKEF010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.216"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            200..742
                     /locus_tag="PWZ48_003066"
     CDS             200..742
                     /locus_tag="PWZ48_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262635.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1272
                     /locus_tag="PWZ48_003067"
     CDS             898..1272
                     /locus_tag="PWZ48_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HDL2262636.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          532 a          148 c          256 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgt ggaatggcaa
     1261 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gct
//
LOCUS       DANKEF010000198         1263 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000198 DANKEF010000000
VERSION     DANKEF010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.217"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..1218)
                     /locus_tag="PWZ48_003068"
     CDS             complement(<1..1218)
                     /locus_tag="PWZ48_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262637.1"
                     /translation="MQTFLTKTSFKKVVLLDYLLEKDNWCAMEELRNLLNVTEKSVLH
                     YIEELEDLFKQYNGNILLKNEDNKRFFIKKEKDFPIYNIYLHFYKASYNYHLIDFMYK
                     YPRSVLKDFAKEQFTSVSTVFRYAKLLIPYFRRYHITFHPFQLELNASEANIRSFFYY
                     FYWNSTRESSDKWPFHIEQKEIEKYIVAFEGIYDITLTIFQKRVFSFWLAINIERSSF
                     RKVRVDNEYKSVISDDPHFNLLKKWSKQINLSFNSDELCFLYRIIYSFGVIDGNAIYE
                     NSHAYAHQRQNTCSYRAVENLEKVLQSMFRFSLDIKDPELIFNFIAFHERSYLFYGNP
                     DLFFNRSYIEEMKEEEPRTYHIMEKLKKELQANADLDVSKKLENWAQLFLDYYYVLDY
                     YDLFLTNVKPIKIL"
BASE COUNT          460 a          197 c          146 g          460 t
ORIGIN      
        1 taaaatctta atcggtttaa catttgttaa aaataaatca taataatcta acacataata
       61 ataatccaaa aataattgtg cccagttttc taatttcttt gatacatcca aatccgcatt
      121 tgcttgtaat tcttttttga gtttttccat tatgtgataa gttcgaggtt cttcttcttt
      181 catttcttcg atatacgaac gattaaaaaa aagatctgga ttaccataaa atagataact
      241 tctttcatga aatgcaataa aattgaatat gagttctgga tcctttatat ctaaagaaaa
      301 acggaacatg ctttgtaaaa ctttctccaa attctcaact gctcgataag aacaagtatt
      361 ttgtcgctga tgtgcatatg catgactatt ttcatatatt gcatttccat caattactcc
      421 aaaagaataa attattcgat aaaggaaaca cagttcatcg ctgttaaaag aaagattgat
      481 ttgttttgac cacttcttca aaagattaaa atgcggatca tctgagataa ctgatttata
      541 ttcattatct actcgtactt ttctaaatga acttctttct atattaatgg ctaaccaaaa
      601 agaaaataca cgtttttgaa aaatagttaa cgtaatatca taaatcccct caaaagcaac
      661 tatatacttc tctatttcct tttgttcgat atgaaatggc cacttatcac tactttcacg
      721 tgtagaattc caatagaaat aataaaaaaa gctacgaatg tttgcctctg atgcattcaa
      781 ttctaactga aatggatgaa atgttatatg ataacgccta aaatatggaa ttaagagctt
      841 cgcatatctg aaaacagtag atacactagt aaattgctct ttagcaaaat cttttagaac
      901 acttctagga tatttataca taaaatcaat caaatgataa ttataagaag ctttataaaa
      961 atgaagataa atattataaa taggaaaatc tttctctttt tttatgaaaa atcgtttatt
     1021 atcttcattt ttcagaagga tattaccatt atattgctta aataaatcct ctagttcttc
     1081 tatatagtgt aagactgact tctccgtaac attaagtaaa tttctaagtt cttccattgc
     1141 acaccaattg tctttttcta acaaataatc taataaaact acttttttga aagatgtttt
     1201 agttaaaaaa gtttgcatca tttcacctca ataagttact tttgtttatt ttaatcaaag
     1261 aat
//
LOCUS       DANKEF010000199         1231 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000199 DANKEF010000000
VERSION     DANKEF010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1231)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1231
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.218"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..858)
                     /locus_tag="PWZ48_003069"
     CDS             complement(<1..858)
                     /locus_tag="PWZ48_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2262638.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKNR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKAC"
     gene            complement(836..1150)
                     /locus_tag="PWZ48_003070"
     CDS             complement(836..1150)
                     /locus_tag="PWZ48_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HDL2262639.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
BASE COUNT          363 a          257 c          166 g          445 t
ORIGIN      
        1 acaagctttt tcccacatgt cgggatcatc aacttcatta tcgttatcca agcgacaaat
       61 aaatggaaac aaactagaaa attctgctcc atccttgtca attccaagtt ctccagaaag
      121 aatcattttt gattcttcta taatgtcatc aagcggacca ccacgaacat gaccattagt
      181 tgtatcataa aattctctat aatctcgatt tttaccacca ccagaagtag ccacatttat
      241 cattgaataa tcttcatttt cgtgaatttc atcaaagcgg tttgcacctg gtcgcttccc
      301 atcttttgtt ctagcatttg ccgtgttata acgaagtttg ctgtttgtag cgatattttg
      361 aataacttcc ttcgtagctt taaatacttt tttatctaaa tcaggatgat ctttaattac
      421 tttaaataca tcatcaaaac tagtctttgc ttggctttca ttattggcat agatatcaat
      481 atcataattt ttaataccgt gtttagcggt tagtagaaag aagttgttcc aagaagcaaa
      541 accagtttta ccattaccac gtcccattaa tgaaagatat ctattgaaca ctagtgtttt
      601 atcttttttc catcgaacac cataaataaa acattgtaga aatttttccc acggaattaa
      661 ttcgaatgga aagtattgtg ctggtatatt gattgaatcc tctaccatct gcttatcgaa
      721 gtaaatatct tctctagtaa agactctttc ttctagataa ttttttagca ataattgctc
      781 tttgcatacc ttgatagtgc cttcttctat agctttgaac caattttcaa tatgcttata
      841 actcaggaat tgattcattt gcttcaccta ccaattcagg agtaatggca agtttatcca
      901 acatcaatcc catttgtttg ttgacagaaa caagcaacgc tactgattca ttctttttac
      961 cattctccag tctaatgccg ttctcggata tatcttcttc cagtgatatc gccgtttccc
     1021 ataaactgat ataacgatca acattatcta agaatggctc aatatttgtt ttctgacttt
     1081 ccaattggct tattaaagag cggcgtaatt tttctctgta gcgattttga gacaattcgt
     1141 ttttaaacat tttagccctc ctttcatgat aaagttcgaa aaaatctctt ttcctgacag
     1201 ccccctccgt ttcatcaccc ccaaaaaatt t
//
LOCUS       DANKEF010000200         1195 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.219, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000200 DANKEF010000000
VERSION     DANKEF010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.219"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1076
                     /locus_tag="PWZ48_003071"
     CDS             <1..1076
                     /locus_tag="PWZ48_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2262640.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          211 g          332 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tcttg
//
LOCUS       DANKEF010000201         1159 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000201 DANKEF010000000
VERSION     DANKEF010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1159)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.220"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            14..1141
                     /locus_tag="PWZ48_003072"
     CDS             14..1141
                     /locus_tag="PWZ48_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2262641.1"
                     /translation="MAFLFVSLNYTNGEDINDDGSRKKVEKVGGDTRPEAEAALRKVL
                     SDIDETGQYFLGTDTRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKL
                     TDLSPALLQNFINAEFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPK
                     YEARPTTKKDLKIISLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVD
                     FSNGTITVEKQMVKNDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYG
                     KLYIDSNAVCTKEDGELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKM
                     KEISERLGHSRISITMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          418 a          200 c          224 g          317 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgaagatat
       61 caatgatgat ggctcaagaa aaaaagtgga gaaagttggc ggagacaccc gaccagaggc
      121 cgaagctgct ttacgaaaag ttttatcaga tattgacgaa acaggacaat actttttagg
      181 tacggatact cgagtaaaac aataccttga tttttggatg gaggaatacg ttaaactaaa
      241 tctaaaatac aatacctatg aaaactaccg atttaccatc aaaaatcata taaacggtta
      301 tttaggaaag aaaaaactta cggatctctc ccctgctctt ttacaaaatt tcatcaatgc
      361 tgaatttaaa aagggttact cgaagaaaac aatgactatt actcactctg tccttaagaa
      421 tgcgctgaat atggcggttt atccttgggg attaatcaag caaaatccta tgctgtatgt
      481 aaaaatacca aaatatgaag cacgaccaac gactaaaaaa gatctaaaaa tcatttctct
      541 tgaggacttt gatcatatgc tagaaattac tcctgaagga catcctttct atattccttt
      601 gaatattgga ttttatacgg gaatgcgcgt tggcgaagtt tgtggtctga cgtgggatga
      661 tgtcgatttt tcaaatggaa caattactgt agagaaacaa atggtaaaga atgacggcgc
      721 atgggtatat ggtacgccaa agacaagcag ttccaatcga acgattttta ttggacaaac
      781 cttgctagca attctgaaaa aacataagaa acaacaatta gaaaatcgaa tgaagtatgg
      841 aaagctctac attgattcaa atgcagtatg tacgaaggaa gacggtgagc tagttacgcc
      901 aagtgtagtg aaatggaata ctaggaggat atcgaatgca ctctccctct cttttaactt
      961 ccattctctc agacatactc atgctacact tcttctcgaa aatggcgcaa aaatgaaaga
     1021 aatctctgaa cgattgggcc acagcagaat ttcaattacg atggatactt actcgcatgt
     1081 aacagataag atgagaaatg aaacggtcga tatcatggaa aatcttagaa agaattcttg
     1141 atttttgcca ccgaaaaaa
//
LOCUS       DANKEF010000202         1151 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000202 DANKEF010000000
VERSION     DANKEF010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1151)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1151)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1151
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.221"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..253
                     /locus_tag="PWZ48_003073"
     CDS             <1..253
                     /locus_tag="PWZ48_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262642.1"
                     /translation="TEGFQETLKTISSTLPQSFKKKFTIFHRYQSGISNAFKVPHSNG
                     VTEGLNNKIKLIKRIAFGYRNFYNFRARIYLQQGLIFEN"
     gene            341..742
                     /locus_tag="PWZ48_003074"
                     /pseudo
     CDS             341..742
                     /locus_tag="PWZ48_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297157.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
     gene            991..>1151
                     /locus_tag="PWZ48_003075"
     CDS             991..>1151
                     /locus_tag="PWZ48_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2262643.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT          405 a          183 c          223 g          340 t
ORIGIN      
        1 tacggaaggc tttcaagaaa cccttaaaac aatttcatct acgttacctc agtcgtttaa
       61 aaagaaattc acgatcttcc atcgctacca atcgggtatt tcaaatgcct ttaaggtgcc
      121 ccactcaaac ggcgtcactg aggggctgaa taacaagatc aagctgatta aacgaattgc
      181 gtttggttat cgcaactttt ataactttcg ggcacggatc tatttacaac aaggtctgat
      241 ctttgaaaac taaaaaaata tgcggcgcac agaacgcgcc gcatatcgga ctaagtttta
      301 ttcaaatttg tcttcgccaa cacaatttga cgaagagcct acaaatatta atagtggaga
      361 attaaataaa gatggctatg tgcaggtggc tagggagatt cacaagaagt ttggatgtag
      421 gtatgtagct acaactctgc gtagaagtta ttcggcaagt agaaatggtt ggagtgcaat
      481 gttatacgaa gcagaaaaag ataaagcgta tttttccaca gaatacaaca tacaaattgt
      541 tgatcgcgtt ggtggtggag acagcttttc tgctggtata atatatgcgt tatgtgaaaa
      601 tatgtccgga caaaaggcta ttgaatttgc gactgcatct agttgtttaa aacatactat
      661 agaaggtgat tttaatcgaa ctacaattga ggatgtatat aatttaatga atagcggtgg
      721 aaatggtcgc gtgttgcgtt agtcaacata taaagataaa tcacagtaat ttttatggtg
      781 attatctgtc ttagtagtga ttagaataac gtgaaaataa attgttgagc gtttatactg
      841 ttttgaaaaa taaatttaac atattgaaaa atacaaatat atacgtatat tataaagcgc
      901 cgattgtaaa attaagctag acaactaaaa cagtcatttg tgctacactc aaaatagttc
      961 cgaccaaaga attataagga gtgagtgcaa ataacctata cctatcttac aacggatgaa
     1021 cttgtaataa tagagtctta tttgaaaatt aaccaatctg ttgctaaaac tgcgcattgc
     1081 ttgaatcgtt caagtcaaac gatccataaa gtatatctgt tcttcaagca aagaaaatca
     1141 gccttagaat a
//
LOCUS       DANKEF010000203         1115 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000203 DANKEF010000000
VERSION     DANKEF010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1115
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.223"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..159
                     /locus_tag="PWZ48_003076"
     CDS             <1..159
                     /locus_tag="PWZ48_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HDL2262644.1"
                     /translation="RRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEI
                     KVLMGITA"
BASE COUNT          419 a          145 c          160 g          391 t
ORIGIN      
        1 cgacgaaata aattttatca aagtctccgt acagcctctt ccacgattaa gggcatggag
       61 accattcgag gaatatataa aaagaaccga agaaatggaa cgctcttcgg cttttcggtg
      121 tctactgaaa tcaaggtatt aatgggaata acagcctaag atatttggag ttcagagagg
      181 gcgcgtttga ttttcaaact tcgcaacaga acccattagc aatatataaa agaaggtatc
      241 aagagtcaca gtgaatttat tccgaaaagt gaaaagagca aatataaaat ttaataaaaa
      301 taagaactaa ataggaaaac gtagacgtaa ttttctacgt tttccttttc atatatatta
      361 aatattacgg aaaattatat aaactaattt agtgttttgt ttgtaattcc taattttgct
      421 ccacttttag ataattttta ggtctattta actcattaga cattctatgc tcatttttct
      481 ccaaatttgt ttcaactctt tcgtacttaa ttttgcgtat ttgaaaattc ttcttttaga
      541 gtacttttat tacgatacta gtatgatata tatctacagc aaacagttaa aatcttccta
      601 aaataagcga atagcattcc taaaacctat aaaattttta gatgaagcga caagtggaca
      661 attaataaac tacttgaaca tgaaattatg gatcaccttt caaatttaga aaatggaatt
      721 cttatactta ttgcgcaaca tttattgatt gcaaaattat gtgacaaaaa gaactagtag
      781 aagaaggaat aatggcttga taataaagta ctacctatta aaggtagtac tttatttaat
      841 ttagtataaa ttaacatact actatatttt aggtttcata ttatataaat taataatata
      901 ctaccatttg ttggtagtat aatttaaagg taataagtta ttcttttatt actatgtttg
      961 ggttttatag caaagaaagt ttaataagag tttggaccta gcaaacaata ttaatgaggt
     1021 cattaaatct tattatcttt agtgatataa tgaaatatct acacgacaaa attatgatct
     1081 tgcgtaaagt tataaaatat taataaaaat atttt
//
LOCUS       DANKEF010000204         1109 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000204 DANKEF010000000
VERSION     DANKEF010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1109
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.224"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            101..472
                     /locus_tag="PWZ48_003077"
     CDS             101..472
                     /locus_tag="PWZ48_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2262645.1"
                     /translation="MTVMRLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPE
                     TASSIRTIELDDNTITFLKAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIV
                     GVHIIRIHGLRHSHASLLISQ"
     gene            complement(756..>1109)
                     /locus_tag="PWZ48_003078"
     CDS             complement(756..>1109)
                     /locus_tag="PWZ48_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2262646.1"
                     /translation="AVNIAAFAMEYLANYLAIRKERYKVTENEKALFLAIYQGEAKRI
                     SGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQLGHSSTNTTTLY
                     THIDNAATIDALNAL"
BASE COUNT          365 a          181 c          192 g          371 t
ORIGIN      
        1 tgatttttgg acaaaagaag aatttacaaa agttatttca actttttata ttgatgattt
       61 gtaccaacat tttggtttta tttgtatgtg gttacttttt atgaccgtta tgcgtttggg
      121 agaagccact gcattaactt ggaaagatat tgattttgaa aataaaaact tgcgtgtgaa
      181 taaaacactc tattataaga gagccaaaac ctatgagttt gtagaacccg aaacggcttc
      241 tagtattcgt acaatcgaac tagacgacaa tacaattacc tttttaaaag cttggaagga
      301 gcgtcaagtt tcaataggag gcatgtcgtt tgtactttct tataatggaa cacctactca
      361 gaaatataca atccgtcata ttatgagaag acatgcaacg atcgttggtg ttcatataat
      421 ccgcatacac ggattaaggc atagtcatgc ttctttgctt atatcacaat aaaaagatgc
      481 acttcgaatt aaagaaaggt taaaacatgc tgatgtacaa acgactttag gaacttatgg
      541 tcatttgtat ctaaattcat tgagagagat aacaaatgat cccacagatt atatccaaac
      601 taatttttct aaaatcaaac cgacacctat ttacgataat aaaaaataaa tgataaacaa
      661 aagtaggatc acaccaaaag aaacacacta gaaaaactat aatacaggct tttctagcgt
      721 gttgtttctt atttccgcct atttatacat tatactcata aagcatttaa tgcatcgatc
      781 gtagccgcat tgtctatatg ggtatataaa gttgttgtat tggtacttga atgtcccaat
      841 tgttgggcag ttaataccaa tgaatttgtc tgttggtaga gtctagtagc taatgtatga
      901 cgcagtttgt gagggctgac ttgaattcta aagccttttg aatattttgc taccatccgt
      961 tcgattgcaa taccggatat acgcttggct tctccttggt aaattgctag aaataaagct
     1021 ttttcgtttt ctgttacttt atatcgttct ttccgaatag ctagataatt agctaaatat
     1081 tccattgcga atgctgctat attgacagc
//
LOCUS       DANKEF010000205         1100 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.225, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000205 DANKEF010000000
VERSION     DANKEF010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.225"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            958..>1100
                     /locus_tag="PWZ48_003079"
     CDS             958..>1100
                     /locus_tag="PWZ48_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDL2262647.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIISRMRDVGDKKVQ
                     FTF"
BASE COUNT          315 a          187 c          232 g          366 t
ORIGIN      
        1 aggtatttgg ctagcaaata tagagcttgc caagcccttt agtgtgtttt gtagaaaggg
       61 accttatagt cactttcaca ttacaaacga tacgtcctat ggacgtatca aaagggtggg
      121 acttatgagc aaattaaagc aaaatttggc tagcaatcct tctaaaaaat acacctattg
      181 atcagagtca aaataaatca gttttcgagt gagcgaatcc aaatttgcaa ctctgaagct
      241 ttgcaaatga gtgtgccggc ttccgtaaaa gtctaggtaa cattagtcga tatatacata
      301 gggctctaat ttgcgtttta aggcgtttta aacgttctgg tatgtaatta taccaaagcg
      361 actttaaaac gccttaaaac gaattttaga ggttctgtag aacgttttag ggttgttagg
      421 gcttgccctg accgcctgta aggcgcttta aaaacaagat aagggatttt atctagactt
      481 gtcttaaaac gccgtagatg agcgctcaga gcttctaaaa ggactcctta cgaactgttt
      541 ttaagcaaag gagcctttag gcgactgcgc agtttaatgt gagagcgtag cgatcacacc
      601 tttaaagcga ccgtagggag cagattccca gtttaggaaa tttcgcgaac ctgtttggag
      661 aaaaaaagta ggaaaatttt aggcgggaaa ttttgccccc ctcttttata atctctttta
      721 taacctcttt ttaaacctct ttaccgtcgg ctgtatcctt actctcccaa ggtgtgcaga
      781 atcttttagg ggtctttttg tctgttttta ggggtctttt tgtctgtttt taggggtctt
      841 tttgtctgtt tttaggggtc tttttgtctg tttttagggg tctttaatta gggggttgac
      901 ccccattaga aagctcttta taattttgtt aagtagttaa tcttgaagga ggattttatg
      961 gcaaatgaaa ttgtgaaata ccaccatgaa ttaaatacga ttcctttacg caaatttacg
     1021 tcagtagaaa tgaatttatt cttctctata atatctagaa tgcgtgatgt aggcgataaa
     1081 aaagtacaat ttacattcga
//
LOCUS       DANKEF010000206         1099 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000206 DANKEF010000000
VERSION     DANKEF010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.226"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..404
                     /locus_tag="PWZ48_003080"
     CDS             <1..404
                     /locus_tag="PWZ48_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HDL2262648.1"
                     /translation="EWVADFAEVQDKILKFAPTMPVEKTEFYVELFREKLAGLLVPTS
                     SGHGSVDLILSECHKTFGLKMLVERLGINPDQCVAFGDGGNDIEMLEYCGLSYAMDNA
                     TEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(477..836)
                     /locus_tag="PWZ48_003081"
     CDS             complement(477..836)
                     /locus_tag="PWZ48_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HDL2262649.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          355 a          190 c          187 g          367 t
ORIGIN      
        1 tagaatgggt agctgatttt gcagaagttc aagataaaat tttaaaattt gctccgacta
       61 tgcctgttga aaaaaccgaa ttttacgttg aattgttccg tgaaaaatta gccgggctat
      121 tagtacctac cagtagtggt catggttctg tagacttgat tctttctgag tgccataaaa
      181 ctttcggctt gaaaatgtta gttgagcgct tggggattaa tcctgaccag tgcgttgctt
      241 ttggagatgg gggcaatgac attgagatgt tggagtattg tggtctgagt tatgcaatgg
      301 ataatgcaac ggaagctgtt aagcaagttg ctaagcatca atgtccttca aatgacgaag
      361 acggtgtttt agtgacgctc gatcgattgt tccctaacac gtaaacacta ggtaattcat
      421 caaaaaacct cgatataaga aatattcgag gttttaaaac tatatttaga acagatttac
      481 gaagcgtctg atgagtcaaa atagacttgg acattgctat ctaaaatacc actttcattc
      541 tttaaacgaa ttgtttgatt tgatggagtt acctctaata ctaaatgccc attaataaag
      601 gtagtatcgt attcagatgt tttaactgtt gcttcttcgc aaggtattgt taaatcatat
      661 gctggaatat aatgttgtgt ggaaactaat aaaaaatgat caccaatcat acagcatccc
      721 tctgctgaag aaaattgatt agagccatca tttaaagtta acaagaatcc atcctttaaa
      781 cgaggtactt gcttaaatac ttctggttgt acttctaaaa ataaattatc gctcattttc
      841 acgaccacca ttgacttcca cagttgaaat tgacatttta tttaacacac ctattccccc
      901 ttaactataa ctcaattcta tataaaaaat attctattct caaataattg aacttttcaa
      961 atcgccaatt tacttcggct ttatcagagc tcattcatat caagagcgat taacgctgta
     1021 aaaattaaac cgaagatctg ataaattttc tctattttct acaatattta aaacgcttcc
     1081 ttagtctatt atatatcta
//
LOCUS       DANKEF010000207         1073 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000207 DANKEF010000000
VERSION     DANKEF010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1073
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.227"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>1073
                     /locus_tag="PWZ48_003082"
     rRNA            <1..>1073
                     /locus_tag="PWZ48_003082"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          329 a          214 c          308 g          222 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga attgagaatg atggaggtag agcactgttt ggactagggg cccatctcgg
      781 gttaccgaat tcagataaac tccgaatgcc attcattcat atccgggagt cagactgtga
      841 gtgataagat ccatagtcga aagggaaaca gcccagacca ccagctaagg tcccaaaata
      901 tatgttaagt ggaaaaggat gtggggttgc acagacaact aggatgttgg cttagaagca
      961 gccaccattt aaagagtgcg taatagctca ctagtcgagt gaccctgcgc cgaaaatgta
     1021 ccggggctaa acatattacc gaagctgtgg agtacacctt taggtgtatt ggt
//
LOCUS       DANKEF010000208         1062 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000208 DANKEF010000000
VERSION     DANKEF010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1062)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.228"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(37..993)
                     /locus_tag="PWZ48_003083"
     CDS             complement(37..993)
                     /locus_tag="PWZ48_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262650.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          177 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aa
//
LOCUS       DANKEF010000209         1050 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000209 DANKEF010000000
VERSION     DANKEF010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1050)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.229"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(501..785)
                     /locus_tag="PWZ48_003084"
     CDS             complement(501..785)
                     /locus_tag="PWZ48_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2262651.1"
                     /translation="MELAFRESLKKMRGTKSKEKFSQELEMSRSNYSRIESGKSDPTI
                     KTLEQIAKLTNSTLVVDLIPNEPTEPEPETESEQVTLDLEMEEEKSNDFV"
BASE COUNT          294 a          177 c          146 g          433 t
ORIGIN      
        1 atctgatttt gtttctaagt agatatttct cttttctaac tctttcttaa acgtttctag
       61 tgtatagata tttgctaatt ttcttatctc caataaacta ttttttatat aagttttaca
      121 ttcatcatga ttcatacaaa ctccaccttc tataaatgaa tacaaaaaaa gcaatcaaac
      181 gatttccgat tgattgctta acaattctta aattcagtag cttagatact tgaaaactct
      241 ctgatttccc tatataatga tagtacggtt atataccgtc ttcaaacaaa gttaattaaa
      301 taacttctta cgagggaaga gttcatctga ctaactgata agcgttggtt tggcaatctt
      361 atcgggctat gcatttataa aatgtcgtca aacattttat aaatgtgtca tggctctttt
      421 ttcgtttcta ttcagttcgt tgtttcgtta tatctagtat accgctttta aaaaaaataa
      481 gcaacgattt cgtgcattat tcacacgaag tcattgcttt tttcttcttc catttctaaa
      541 tccaatgtta cttgttctga ttctgtttct ggttctggtt ctgttggctc atttgggatt
      601 aaatccacta ctagcgttga gttagttaac tttgcaattt gttctagtgt ttttatggtt
      661 ggatctgatt ttcctgattc tattcgtgaa taatttgatc tactcatttc taattcttgg
      721 gagaattttt cttttgattt ggtacctctc atctttttta agctttctct aaatgctagt
      781 tccaagtttc tcccccctct ttttgtgtaa taaaattcac gctttattta aattatagct
      841 tttaatatct taataaacaa gtgattaatc acttgtgatt tgtgattatt ccaaaagtag
      901 gtaatttcac caaccttttt caaaagtttt tatcgacgat tattggactg gatcgtgttt
      961 cctaaagcga ttctaagcgc atgtttaatt ttaaagtggg taattctctt taaaacgtcc
     1021 tacaaagatt caaaaaaagc atggctgcta
//
LOCUS       DANKEF010000210         1049 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000210 DANKEF010000000
VERSION     DANKEF010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.230"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            4..978
                     /gene="bsh"
                     /locus_tag="PWZ48_003085"
     CDS             4..978
                     /gene="bsh"
                     /locus_tag="PWZ48_003085"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HDL2262652.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          375 a          132 c          182 g          360 t
ORIGIN      
        1 actatgtgta cgtctattac ttatgtaaca agtgatcatt attttggaag gaattttgat
       61 tatgaaatat cttacaatga agtagttact gttactccaa gaaattataa gttgaatttt
      121 cgaaaggtaa atgatttgga tactcattat gcaatgattg gtattgccgc tggtatagct
      181 gactaccctc tttattacga tgcgacaaat gaaaaaggat tgagtatggc tgggctaaat
      241 ttttctgggt atgctgatta taaagaaata caagaaggga aagacaatgt atctcctttt
      301 gaatttattc cttggatttt aggacaatgc tcaacagtag gagaagctaa aaaattgtta
      361 aaaaatatca atttagcaaa tataaattat agtgacgaac ttcctttatc ccctttacat
      421 tggctattag ctgataaaga aaaatcaatt gtcattgaaa gtatgaaaga tggacttcat
      481 atatatgata accctgtggg cgttcttacc aataatcctt catttgacta tcaattattt
      541 aatttaaaca attatcgtgt cttatcgagt gaaactccta aaaataattt ttcaaatcaa
      601 ataagtttga atgcctatag ccgcggtatg ggagggatag gcttgcctgg agatttatcc
      661 tcagtatctc gttttgttaa agcgactttt acgaagctga attctgtatc tggagattca
      721 gagtcagaaa gtattagtca atttttccat atcttaggtt cagtagaaca acaaaaaggt
      781 ttgtgtgatg taggtgatgg aaaatatgaa tatacaattt attcttcttg ttgcaatgtt
      841 gacaaaggaa tctattatta tcgaacatat gaagacagtc aaattactgc aattgatatg
      901 aataaagaag acttagatag tcataagtta attagttatc caattataga aaaacaacaa
      961 attaaatata taaattagtt aatgtgttgt gattgattat ttaatataga tataatacaa
     1021 aaaggcaaag atattttcaa aacattttt
//
LOCUS       DANKEF010000211         1046 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000211 DANKEF010000000
VERSION     DANKEF010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.231"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            1..505
                     /locus_tag="PWZ48_003086"
                     /pseudo
     CDS             1..505
                     /locus_tag="PWZ48_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            828..944
                     /locus_tag="PWZ48_003087"
     CDS             828..944
                     /locus_tag="PWZ48_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HDL2262653.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          396 a          147 c          153 g          350 t
ORIGIN      
        1 atttcaattc tgagtttggt caccttgaag gtgatacgat cgttggcatt catcataaaa
       61 gtgccgtcat tactttagtt gaaagattat ctaaagtcat tatcacgatt aaacccaacg
      121 gccgtaaggc attagatatt gaaactgccc ttaatcaatg gttttctcgc ttccctaaaa
      181 acttctttaa atctattacg ctattacgtt tgactgtgga aaagaatttt ctaactggaa
      241 agccattagt aaccaacatg atattgatat atattttgcg gaccctggaa caccttctca
      301 acgcccatta aacgagaatt ctaacgggat tctgcgtcgt aatggactgc cgaaatcaat
      361 ggattttaga gaagtgaatc agacatttat ttccagtgtc agcaatcaac gtaatcatat
      421 tccaagaaaa tcattgaatt acagaacacc aatcgaaata tttttgagct atgtacaaga
      481 agcattttat tctagcttaa tttgacaaat cataaataat aaagaattga acttctgaga
      541 aaacaaagaa tcttttttct ttttttgaat tgactcatgg attggcgtta aaattttatt
      601 ttgaaaaagt tatttctggt ttaactcaaa aaaagagata tgaatctaat agtgatagaa
      661 aggatgttat ttttcatgaa atggttgttt tagatttaat acatataact aataaatgct
      721 attgaatgta tttaaactaa ttaacaaaag aatttgaatt gttaataaaa tccaataaaa
      781 tatattaata aagtagatag aaataaaaaa acaaagacta aacatatata attggattaa
      841 acgcaatatc tgcaaaatat tgtaaattta aagcctttgt agaaaaattt actgaaagac
      901 aaactgataa gattattcag tatcatatta cctatactga ttaaaatttt ttgaaacggg
      961 atactaaata aaaaatttat ttataacaga actaattatt taatgcggat gcgccaatac
     1021 ttggtcctgc aagagctatt tatttt
//
LOCUS       DANKEF010000212         1042 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000212 DANKEF010000000
VERSION     DANKEF010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.232"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..472
                     /locus_tag="PWZ48_003088"
     CDS             <1..472
                     /locus_tag="PWZ48_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010744709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HDL2262654.1"
                     /translation="DQIKTAVLYPHNGLPILLMGVSGSGKTFLAEKIYEYAKEQQVIE
                     SNAPFIAYNCAQYFNFSINKIVENLTILDAMKVLRLVQKAVKELEKLMGIEFSNNQLF
                     LLYMHCCSMIERILRKESVDEQADIKEYIQKEGHNYEISKYPAYKTKFESTVIK"
     gene            complement(679..>1042)
                     /locus_tag="PWZ48_003089"
     CDS             complement(679..>1042)
                     /locus_tag="PWZ48_003089"
                     /inference="COORDINATES: protein motif:HMM:NF013068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262655.1"
                     /translation="KEDFKAIFKTASIELAGERKQLIMDKYGEDKKVATACKILDEGF
                     EDAMQVMVLPENIRQRLRTTNVLERLNEEVRRRERVVRIFPNCDSVIRIIGALLMEKD
                     DEWTSAPRKYLEFDRNEI"
BASE COUNT          342 a          194 c          194 g          312 t
ORIGIN      
        1 cgatcaaatc aagacggctg tcttgtatcc tcataacgga ttaccgattt tattgatggg
       61 tgtaagcggt tcaggaaaaa cctttttggc agaaaaaatt tatgaatacg ccaaggaaca
      121 acaggtgata gaatcaaatg ctccatttat cgcctataat tgtgctcaat actttaattt
      181 ctcgattaac aagatcgtag aaaatttaac gatccttgat gcaatgaagg ttttgagatt
      241 ggtacaaaag gccgtcaaag aactcgaaaa actaatgggg atcgaatttt caaacaatca
      301 attgttcttg ctttacatgc actgctgctc aatgatcgaa cgtattttac gcaaagaaag
      361 tgtagatgaa caagcagata tcaaagaata tattcaaaaa gagggacata attatgaaat
      421 atcaaaatac ccggcttaca aaacgaagtt tgaaagtact gtaataaagt aagatttaaa
      481 taagcataaa caaggtaaga accatagggg ctgtgtcaag tccaaaatct tgtgtaaaaa
      541 ggaaatctca aagtacgatg ttttgccttg ttgagcctag ctacggtcca atatgctggt
      601 aagaagtgtc aagagcaaaa agcgaagcgt ctcttgatac ttctcaccag tatattatac
      661 tattggcggc ttaatgtgct aaatctcgtt acgatcaaat tcaagatatt ttctaggtgc
      721 agaagtccat tcgtcatcct tttccatcaa taaagcaccg atgatacgaa taacagaatc
      781 acaatttggg aaaattcgga caacacgttc acgacggcgt acttcttcat ttagtctttc
      841 aagaacattc gttgttctca atcgttgacg gatattttct ggtaaaacca ttacttgcat
      901 ggcatcttcg aagccctcat ccaatatttt acaggcagtt gcgactttct tgtcttcccc
      961 atatttatcc ataataagtt gtttacgttc tccagccagc tcgatgcttg ccgttttgaa
     1021 gatggcttta aagtcttctt tg
//
LOCUS       DANKEF010000213         1019 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000213 DANKEF010000000
VERSION     DANKEF010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1019
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.233"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(136..408)
                     /locus_tag="PWZ48_003090"
     CDS             complement(136..408)
                     /locus_tag="PWZ48_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262656.1"
                     /translation="MSDGFEQVSIIKNQVREILRKKGYLVDSYFEGDYETWVGVYARP
                     ENKPTYLDPTTSEDAYLQNRYRVDGFKQDFAEWFEWEIENGEVKEE"
     gene            complement(491..742)
                     /locus_tag="PWZ48_003091"
     CDS             complement(491..742)
                     /locus_tag="PWZ48_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262657.1"
                     /translation="MRKVLVFNTRGQIFLGIEGTRVTVDGWCDCFEVGKHVELMAFTE
                     LKDKNNVEFFESDIGWDDHEEVYGKFILRKGNSYTNGEA"
BASE COUNT          312 a          240 c          103 g          364 t
ORIGIN      
        1 ttacaggatc tggcttaaga tcttctggaa gagaaatatc aatcatacca taatgatccg
       61 attcctcagt attattgtca ttactagcta ctggtttggt tgttctactg gcttattttg
      121 atacaacaag tactcttatt cttcttttac ttctccgttt tcaatttccc attcaaacca
      181 ctccgcaaag tcttgcttaa aaccatctac tcgataccta ttttgtaaat aggcatcttc
      241 tgacgtcgta ggatctaaat aggtaggttt attctctgga cgagcataga cacctaccca
      301 tgtttcgtaa tctccttcaa aataactatc taccaaatag cctttttttc taagaatctc
      361 ccgaacttga tttttaatta ttgatacttg ttcaaaacca tcactcattc tatcacctca
      421 aataactcta gattctcata aatattcccg acaatttcaa tcctatcaat gatctctaac
      481 aaatcgctac ttatgcttcc ccattcgtat aagaatttcc cttccgcaaa ataaattttc
      541 cataaacttc ttcatgatcg tcccaaccaa tatcactctc gaaaaattcc acgttgttct
      601 tatcttttag ctcagtaaat gccataagtt caacatgttt tccaacctcg aaacaatcac
      661 accacccatc aaccgttaca cgtgtgcctt caatacctaa gaaaatttgt cctcgtgtat
      721 taaacactaa aactttccgc attttttgct cattaattaa tcacgcgcga aatctaggaa
      781 tcacaacttg ttcgcttctt ctttataaaa taaaaaaact ccaataatcg gaaattacta
      841 tcttttcctc attccttttt taaatcttca tacttcttct tatctttgtc ctaacctaat
      901 aacacaaatt ttgaagaaaa actcagtaaa acctgattct tttttatctt tacaacaacc
      961 aactttttct tcagatcctt ttactagaac ttgtatacaa aaattgttga atgctgata
//
LOCUS       DANKEF010000214         1006 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000214 DANKEF010000000
VERSION     DANKEF010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.234"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            35..532
                     /locus_tag="PWZ48_003092"
     CDS             35..532
                     /locus_tag="PWZ48_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2262658.1"
                     /translation="MNNQIALAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(569..994)
                     /locus_tag="PWZ48_003093"
     CDS             complement(569..994)
                     /locus_tag="PWZ48_003093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262659.1"
                     /translation="MDPAIVIPQYFAQLSEALVVIEADVFAHFDYGFRVFGLSVAEFK
                     QYEAQFLPILDQVIKNKLAFELNAKSAYLYDNLALYEYVIDLYLSRGGTLFSVGSDGH
                     YLEHFRFHFDDLFALLKANGVTELAIYQKGKRIMVPLPV"
BASE COUNT          360 a          167 c          178 g          301 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatcg cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt agactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac catttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt gattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttc
//
LOCUS       DANKEF010000215         1001 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000215 DANKEF010000000
VERSION     DANKEF010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.235"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..>1001)
                     /locus_tag="PWZ48_003094"
     rRNA            complement(<1..>1001)
                     /locus_tag="PWZ48_003094"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          210 a          311 c          227 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatgcaccgc g
//
LOCUS       DANKEF010000216          998 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000216 DANKEF010000000
VERSION     DANKEF010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..998
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.236"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(80..634)
                     /locus_tag="PWZ48_003095"
     CDS             complement(80..634)
                     /locus_tag="PWZ48_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDL2262660.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT          323 a          185 c          107 g          383 t
ORIGIN      
        1 ttccgaaaat tcagttatct tcattaatct caccttctaa gttttcatta tacacaaaaa
       61 atatatccta atatctcaat tagcctccca atttaaaacc acgtaacgat gcccctattt
      121 catcttcagt tataccaatg tacctttttg taattgcttg cgagctatga ttaaaaataa
      181 acattaaggt agcaatgtct ctagttttct tgtagtagtg atacccaaaa gtttttctta
      241 atgtatgtgt tcctatatca tctcttccta atttttctgc tacatctgca tatatctgat
      301 agacacgatc ccttgttata tgtcgacctt gttgccgact tggaaataac caatcatttt
      361 cttctttacc ttcagtatat aataaaatct cttctctcaa attatctata aaaatcggtt
      421 ttcgcttacc tgttttatgt tcaacaataa taggcttact tttacctcgt atgtctccaa
      481 cttttaatcc tataatatct gacatacgta acccattatt gattccaaac aaaaataaaa
      541 aactatctct ttctggatta cttgttcttc ttagccaaaa ctgaaaatct tgtatctctt
      601 catttgtcct taacggttgt acattatagc tcactattgt ttcccttctt aaaaatattc
      661 tgagttcatg attatatata tattttacca taatcatgaa cttatatata tactctgtta
      721 aaagtgaaga aaatcaaggg gtttagttta tgattttata tcatttcagt aactcagaat
      781 attccgactt ttttgtacaa aaaaattaac cttacttata tgaagattct aagctcttat
      841 ttaaatttta gacgggcagt tatccgtaga agttcttaga atagctaatt tacataatta
      901 aattatatga aatttaagtt tgaacaatat actaagaact ccccttacta taaagtgaaa
      961 taaattagca tatgaataac cgtgctacca aacttttc
//
LOCUS       DANKEF010000217          942 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000217 DANKEF010000000
VERSION     DANKEF010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.239"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            104..>942
                     /locus_tag="PWZ48_003096"
     CDS             104..>942
                     /locus_tag="PWZ48_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HDL2262661.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMY"
BASE COUNT          351 a          135 c          161 g          295 t
ORIGIN      
        1 aatataaaag ccctttaaag agggctttta tatattaatc acaaatcact tatcacaaat
       61 cacaagtgat taatcacaaa tcacttgtga tttgtgattc ttaatgatac aatattacta
      121 tacaaaaaaa gaatggggcg tagttatgga gaaggaaaaa ctaaaaatac ttgaagaatt
      181 aagacgtatt ttaaacaata aaaatgaagc aattattatc ttgaataatt actttaaagg
      241 tggtgttgga aagtccaaat tatcgactat gtttgcttac ttgacagaca aattgaattt
      301 aaaagtttta atgatcgata aggacttaca ggcaacattg acaaaagact tagcaaaaac
      361 ttttgaggta gaattgccac gtgtcaattt ttatgaaggc ttgaaaaatg gaaacttggc
      421 ttcttctatt attcatttga ctgataattt agacttgatc cctggcacgt ttgatttgat
      481 gttactgcca aaattaactc gctcatggac gtttgaaaat gaaagtagat tgcttgctac
      541 tcttttagca cctttaaaaa gtgactatga tcttattatt attgatactg taccaacgcc
      601 aagcgtttat acaaataatg caatcgtggc gagtgattac gttatgatcc ctttacaagc
      661 agaagaagaa agtacaaaca acattcaaaa ctatatttcc tatttgattg atttgcaaga
      721 acagtttaac cctggactag atatgattgg ttttgttcct tatttagttg atacggacag
      781 cgcaacgata aaatcaaacc tggaagaact gtacaaagaa cataaagagg ataatttggt
      841 tttccgaaat attatcaagc gaagtaataa agtaagtact tggtctaaaa atggcattac
      901 agaacacaaa ggctatgaca aaaaagtttt gtctatgtat ga
//
LOCUS       DANKEF010000218          929 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000218 DANKEF010000000
VERSION     DANKEF010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.240"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>929
                     /locus_tag="PWZ48_003097"
     CDS             <1..>929
                     /locus_tag="PWZ48_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IA DNA topoisomerase"
                     /protein_id="HDL2262662.1"
                     /translation="FSLGRVQTPTLYMIYQLQEKIRNFQKEPYFEGKAQVIAQNGAFD
                     AKLDPNETQATQEAFEAYLKEKGVQLGKQPGTILQVETEKKSAASPRLFSLSSLQSKM
                     NQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEGEYAYLLDHLDEYKHFLKAEAI
                     PTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQLSPLQQAIYEQVLKTTVAMFA
                     EKYTYEETTILTQVQQLQLKAIEKVPLDLGWKKLFGKESDGKEKEEEPLLPKVTKGEM
                     VTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTV"
BASE COUNT          337 a          179 c          203 g          210 t
ORIGIN      
        1 ttttctttag gtagagtcca aaccccaaca ctctatatga tttatcaatt gcaggaaaag
       61 attagaaact tccaaaaaga accatatttt gaaggaaaag cccaagtaat cgctcaaaat
      121 ggcgcatttg atgcgaagct tgatccaaat gaaacccaag cgacacaaga agcatttgaa
      181 gcctatttaa aagaaaaagg tgttcaatta ggaaaacagc caggtacgat ccttcaagtc
      241 gaaacagaga aaaaaagtgc ggctagccca cgtctttttt ctttatcgag ccttcagtct
      301 aaaatgaatc aactcatgaa agctagtgcc aaagatactt tggaagccat gcaagggttg
      361 tatgaaggaa aatatctaag ctatccgcga acggataccc cttatattac ggaaggggaa
      421 tatgcgtatc tgttggatca cttagacgaa tacaagcatt ttttaaaagc cgaagcgatt
      481 ccgactccta ttcatacacc gaatagtcgg tatgtgaata ataagaaagt ccaggaacat
      541 tacgcgatca ttcctacaaa aacggttatg acagcagcgg cttttgaaca actaagtcca
      601 ctgcagcagg ctatttatga acaagtgtta aaaacaacgg tggccatgtt tgcggaaaag
      661 tatacctatg aagaaacaac gatcttgacg caagttcagc agcttcagtt aaaggcaatc
      721 gaaaaggtgc cgctagatct aggttggaag aagttatttg gaaaagagag cgacggaaaa
      781 gagaaggaag aagagccgct acttcctaaa gtgacaaaag gagaaatggt gacggtggat
      841 ctacaagttc ttgaaaaaga aaccaagcca ccacagcctt ataccgaagg tacgttgatc
      901 acggcgatga aaacagctgg gaaaaccgt
//
LOCUS       DANKEF010000219          911 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000219 DANKEF010000000
VERSION     DANKEF010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.241"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(602..675)
                     /locus_tag="PWZ48_003098"
     tRNA            complement(602..675)
                     /locus_tag="PWZ48_003098"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(639..641),aa:Ile,seq:cat)
BASE COUNT          309 a          151 c          158 g          293 t
ORIGIN      
        1 gactgacgtg ccggacgcac gtagcgatca tcggcgttat gactatataa agtatacttt
       61 cgttgatcca gaaaggtata aagattaaag taggctacct aaaaaggtag cccggaacga
      121 attttatcac catacttgtg aaaggagata ttttttaagt tagtattaag attgtgtaat
      181 atgatgatat ctatatttta tagtatcagt gctataaaat caaaaataag tcactaatta
      241 actaccactc caattgtaag ccttttttct cacttttttt caaaaatatg gtatgctttt
      301 taatggcttc aaatataaaa gagtttaaag cgtaacacac ttatggggaa gtggtttggg
      361 gtgcgcttta aactcttctt tattattatc tcacaattta acccaaatgt ctttctattt
      421 aaaaatcaaa gtaaaacttt tcaaatatac agaagtataa ctatgtgaaa catcctttca
      481 ttaatccata aaaggataca taaaaaagcc actcatttga gtggcaatga agaaaagctt
      541 tagcttgtat aatactcttc aaaaaaattc taacacagaa cgattcaaat ggctacgtta
      601 atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg ctctgaccaa
      661 ctgagctaag ggtactggtt gttgccacat aaagccataa acaatcaacc agtagaatgt
      721 gtggcaacaa acctgttatc gcatagcttg gagtgtgact atttatgggt gatagtgaag
      781 atatgcgata acatcactat tttatcgaat aatttttata gttgtcaata tagttatgta
      841 ctgctcctca acgaggagct atttttatcg tttaggaata tttaaatacc aacgtttgtc
      901 atggaaatct t
//
LOCUS       DANKEF010000220          897 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000220 DANKEF010000000
VERSION     DANKEF010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.242"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(120..596)
                     /locus_tag="PWZ48_003099"
     CDS             complement(120..596)
                     /locus_tag="PWZ48_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDL2262663.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(609..872)
                     /locus_tag="PWZ48_003100"
     CDS             complement(609..872)
                     /locus_tag="PWZ48_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIB"
                     /protein_id="HDL2262664.1"
                     /translation="MNKNTSLHEESLIIIEALGGKENIESVEACATRLRVAVTNGEII
                     QKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
BASE COUNT          297 a          166 c          117 g          317 t
ORIGIN      
        1 gggaatcatt actaataaaa taaaaaaatg aacagaaaga aaacagttct ttctgttcac
       61 caacttcatt tgacaaatat cctatttagc ttattttata gctttttata tacacttttt
      121 taaaaagtga tttttccaca tgaattccca tgagcaattc gaccattatc tattattaaa
      181 tcattgaact gatttttctc cggaaaaaca actataacag aagtatcttt accagatttt
      241 attattttgt caaaatccac atcaaccaac gaggtttcag gagtaacctc atcattttct
      301 tgaacacaaa tcgaaaaagg ctctccttgt aattcaactg tatcaattcc aatatgaatc
      361 aaataatcaa tgcctgagtt gctttttata ttaatagcat gtttagtagg aaatatattt
      421 gttactcgac cagccaatgg agcataaatt tttgaaccat caggtatgac ggcaaaacca
      481 tcacctagta atttttgtga aaagacagga tcatttactt ctgtgatgga aatgattttt
      541 ccggaacaag gtgcataaaa agtttcttgt tttttctttt tgaaaatttc aaacatatta
      601 aaaacctcct aattatcttt tcctaatatt tgattgattt cttgacttaa taagtcagct
      661 ttccctccaa aaacagcttg gatgccaccc ttcacttcga agacagcagt tgcaccagtg
      721 tcttttattg tttgtttttg aataatctcc ccattcgtaa cagcaacacg caaacgagtt
      781 gcacaagctt caacagactc aatattttct tttccaccaa gagcttcaat aatgataaga
      841 gactcttcat gtaatgatgt atttttgttt attacttttt cagaatgaac ttctgat
//
LOCUS       DANKEF010000221          894 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000221 DANKEF010000000
VERSION     DANKEF010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..894
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.243"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            252..875
                     /locus_tag="PWZ48_003101"
     CDS             252..875
                     /locus_tag="PWZ48_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDL2262665.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          329 a          129 c          167 g          269 t
ORIGIN      
        1 aactaatgtc cttatttttg tgttcacatt tctgttacct aagaaatact taaaggaggt
       61 gatgtagcca gcaacgacta caacttgaac ccgctatcta cagtttattt gttgtttctt
      121 ttgggtatac tctaatggta caaatccata tttgcttaaa gcgttgattt ttcagcggtt
      181 tttctttatt gcaaagtgta ccctaagacc tgtatactaa tagtaaatag attggatagg
      241 aatgatggca aatgtattta gcttatttaa gagtttcttc cgacgatcaa agtttagctc
      301 ggcaacataa gctaattgaa aattggtcag tcaaaaataa tattgctgaa gacgatttaa
      361 aaattttcga ggaaaaagtc tctggaaaaa atattgaaaa cagaccagag ctgcaagagc
      421 ttatttcttt catacgtgaa cgagatacga ttattattcc tagtcttgat cgattagcaa
      481 gaaatagtaa agatacaaaa gacttacttc aattgtttcg tagtaaaggt gcttctattg
      541 aaatattaga cctaccgtcg tttaatgggg taactgaccc tgcattaaga gacttgctaa
      601 caaatttagt catagaagtg tttagctatg ttgctgaaaa cgaacggaaa aaaatcaaag
      661 agcgtcaaaa acaaggcatt gaaattgcaa agaaaaatgg tcgttatact ggtaaaaaga
      721 ttcaatacca tgccaatgca acaggaaaaa acaaattgat ttacgatgaa attgttgatg
      781 gtctgaagaa aaaagaaact atttctaaaa tagcaaagaa cgcaggagtt acaagaaaaa
      841 ccgtttatcg agtaaaaaga gatttaggaa tgtaatatgt tccagttaga aaga
//
LOCUS       DANKEF010000222          864 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000222 DANKEF010000000
VERSION     DANKEF010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.244"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..638)
                     /locus_tag="PWZ48_003102"
     CDS             complement(<1..638)
                     /locus_tag="PWZ48_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2262666.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSN"
BASE COUNT          267 a          168 c          142 g          287 t
ORIGIN      
        1 ttgttggacc gatctaaaat atctccgtcc gattgctctt cgtagcaaag catcaaatca
       61 ctatcaatga ctacatcagt tcctgctact aagccttcga aactaaacac ataatcattt
      121 aagatgaact ggcatgaagt agctgaagga gtgatgataa tctttggaaa actttcttct
      181 aaactattat tcagcaagtt aaatgactgt ggtttatcta cggttatagg tacatcttct
      241 tgaactcttg cgaatggttt cgcagtaata tttacatcga actctcccca ttcaacaata
      301 tcgttttctg catccccaat atcgatagtc tggataacgt aatagacgtt gggatcgtca
      361 gagaattcta atttctttgc atagtttaac caatgacgca tgatataaaa cgattgcttg
      421 aacgcttgat ggtcttccac atcctctaaa tagttatagt gcaatgtaaa cgacatatct
      481 tcaaacgagt aatcttgtac taagccacct aacctaccta aaacagaagt ttcaactctc
      541 tgtctttttg gagaaggtat ggttggtctt tcagctaaag ccaatttatg caaataatca
      601 ggaaatccat cgattataga atgtatacaa tcagtcattt tttcacatcc tttttaatac
      661 taaaaaaaca ggagaaatac tctcctgttt aacgccatgc cgaagcatta tcattttgaa
      721 tttttgtaat gctatcaatg atttgttgag ttgtttgctt catagtaacc tcatctgcgt
      781 taccatcaat tgtgaaattg aattcgtagt tattcacagg ttgaatcgtt tgtgccctag
      841 atgaaactga tgtgctactc aaga
//
LOCUS       DANKEF010000223          860 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000223 DANKEF010000000
VERSION     DANKEF010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..860
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.245"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..752)
                     /locus_tag="PWZ48_003103"
     CDS             complement(<1..752)
                     /locus_tag="PWZ48_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005470778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDL2262667.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKA"
BASE COUNT          258 a          171 c          135 g          296 t
ORIGIN      
        1 tctgccttcg acttattttt agcctgtgcg ttcttcgcta attgggcagc agctcgtgct
       61 aagtaataag tcaaatggtt atccgaacaa atatcgattg cgtaatttag tagtgcatca
      121 cttgtttcca aatcaccgtc ttgcgcaaaa aaaacaccac aatgaaagac gatatttaac
      181 acccgccaga tatcttctgt tgtacgaatc ggatacgtat aaattttttc aaacactcga
      241 ttgaaataaa cttctgcttt ttccaactct ttttctctag catagaccat tccaatacct
      301 gtatatgcca ataaacgaaa caacgtgttg gctgtttctg ccatcaacaa ctgatcaaaa
      361 tggaacaaca catccgtaat tggtgcattc gtaaaaatca tttcaaatcc ttttagatag
      421 tagtaacgtg caatcagctc agactcgtca attaccttgg gatcaatggc tgctaaaatc
      481 tgtgtcaatt gttcatattc actcgtaatc agtgaaaact ctgctttttc tagctgttca
      541 tcaagttgtg tattgatcac gttcatcgaa gaaaaaagtt caactaacgg aagattgatt
      601 cgattacata atttgattaa aatttttaac gaagggactt gaccattgtt ctcaaattta
      661 cttaatgttg cttgtgtaca aattccttca gacacttctt tttgagataa gttggcagcc
      721 tttcttgccg taataaattt ttcaatgttc attattttct ctccaatcag acctcacacg
      781 aaaatcattt ctgtctcgat tttagcatgt tttctttggg aatgctttag gttctgttgc
      841 aaagttttaa ataaagaata
//
LOCUS       DANKEF010000224          853 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000224 DANKEF010000000
VERSION     DANKEF010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..853
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.246"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            411..853
                     /locus_tag="PWZ48_003104"
                     /pseudo
     CDS             411..853
                     /locus_tag="PWZ48_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          305 a          148 c          156 g          244 t
ORIGIN      
        1 tttatcacgg atgagcttga aatcgtcttt tatacgtatc aaattgaaat gttcggcata
       61 caacctatcg ttgttactac attactaatt gtcacttgtt aaggaacttg gatcctttct
      121 aaacattccc tagagataaa ttttacttta tccctccgtt aatatccaat ttaagcacct
      181 ttccgattca gggttaattt gcatatcaag tgcagcttgt gttctagtaa gattagccct
      241 tttagtccgg ctgagttgac ttatggatat aagatatggt catagcaaat agatactacg
      301 tatagtctgt taatttagtg tcattaatct taagttggat aggctacaat taactagaca
      361 gaaaaattaa ggtgtgtaga ctagaagaaa acataccagg aggaattttt atgtctaaga
      421 gaacacgaag aactttttca caagaattca agcaacaaat cgtcaatctt tacttagctg
      481 gaaagccacg tgtagaaatc attcgagaat atgaactaac ggcttcagca tttgacaaat
      541 gggtaaagca atctaaaacg agtggttcat tcaaagaaaa agataatcct acgcctgaac
      601 aaaaagaatt gttagaacta cgtaaaagaa accagcaatt agaaatggaa aatgatattt
      661 taaagcaagc agcgctgata ttcggacgaa gagacaagta atcgatgcga ataagcatct
      721 ttaccctata tcagcgatgt gcagaatatt aggtctatca cgtcagtcct attattatca
      781 atcaaaacca aagaaagacg aatcagaact tgaagaagca gtcgctgaag aatttatccg
      841 cagccgaaag gcc
//
LOCUS       DANKEF010000225          843 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000225 DANKEF010000000
VERSION     DANKEF010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..843
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.247"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..794
                     /locus_tag="PWZ48_003105"
     CDS             <1..794
                     /locus_tag="PWZ48_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HDL2262668.1"
                     /translation="EYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVASYDDVLHTKEKE
                     FPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALRLQNIKREVIDI
                     SIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILKPALEEAGFTVE
                     IVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISNGNIRYEKIEPE
                     TRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
BASE COUNT          290 a          116 c          173 g          264 t
ORIGIN      
        1 aagaatactc tcaaatgcaa cgtaatgctg gtttaagact tacgttcatg accaaacgaa
       61 tgaacagacc tatggaagat acacgatttg ctgttgcttc atatgatgat gttctgcata
      121 cgaaagaaaa agaatttcct gaaaaaatgg atgaagtgat aggaacagtc gattttgctg
      181 atagacgaga ttttgccagc gttgggttgc taggaaaata cgataaagat gtttatttta
      241 cacaacatac ttttatccac gaatcagccc ttcgattaca aaacatcaaa cgagaggtta
      301 tagatatttc tatagatcaa ggaaaatcac agatcgttca tggaaaaaat atagaagctg
      361 attatattgt aggttggttt cttgaaatga gtaataaata ttatattaaa aaaatcgcta
      421 tggatatgta ccgtgcaaaa atattgaagc ccgctttaga agaagcaggt tttactgtgg
      481 aaattgttcg aagcggatct gttacacatg gtatgttaaa agatctggtt gatgaccttt
      541 ttattaatca acgtttattt tttggtgacg atgcgattat gcgttggtat tgcatgaatg
      601 tatatgaaga gcatatttct aatggaaata tacgctatga aaaaatagaa cctgaaacta
      661 gaaaaacgga tggctttttt tcattccttc atggtttgaa ttttttagat gatatttatg
      721 attctgctcc tgtaacagtc acaaatagct cagtagaaaa tacaggaact ggatttactc
      781 ctctagtatt ctaacttgaa aggaggtgag aaagtgggga tttttcaaaa ggcggtagga
      841 tac
//
LOCUS       DANKEF010000226          829 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000226 DANKEF010000000
VERSION     DANKEF010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.248"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..720
                     /locus_tag="PWZ48_003106"
     CDS             <1..720
                     /locus_tag="PWZ48_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDL2262669.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          319 a          128 c          165 g          217 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatca
//
LOCUS       DANKEF010000227          816 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000227 DANKEF010000000
VERSION     DANKEF010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..816
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.249"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            1..797
                     /locus_tag="PWZ48_003107"
                     /pseudo
     CDS             1..797
                     /locus_tag="PWZ48_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
BASE COUNT          285 a          152 c          148 g          231 t
ORIGIN      
        1 actacacgcc ccaaaccatg tccaacatga ctaaagtgct gactgaagaa gtaaatgcat
       61 ttaaagttag aactctaaat gataagtatg tcgctatttt catggatgct acttatattc
      121 ctttaaaacg gcaaaccgtc tccaaagaag caatctatat tgccattggt atacgagaag
      181 acggcactaa agaagtacta agttatgcga ttgctccgac tgaatcgacc tacgtttgga
      241 atgagttgtt acaggacatt tactccagag gggttcatga tgtcttactg tttattaccg
      301 atggcttaaa aggcatgaaa gatacgattc accagattta tcctaaagct aaataccaac
      361 attgctgtgt tcacgtttcc cgtaatattg ctcataaagt acgtgtcaaa gatcgaaaag
      421 aaatctgtga tgattttaag gctgtttatc aagctagctc aaaggaagag gcaaatacct
      481 ttttagggag tatgattgag aagtagcaaa aaacttatcc taaagtgacg cagtcactga
      541 taaaaaatca agatttattg actttttatg agtttccgcc tggtatccgc cgaagcattt
      601 actcaactaa tttaatcgaa tctttcaaga aacaaatcaa gaaatacagc cgcagaaaag
      661 agcagtttca aaatgaagaa tcactagagc gtttcctagt atccatcttt gatacataca
      721 atcaaaaatt cttaaataga agccataaag gcttccaaca agtgacggat acattagctt
      781 caatgtttac tgagtaaccc attattttgc agaagg
//
LOCUS       DANKEF010000228          815 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000228 DANKEF010000000
VERSION     DANKEF010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.250"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>815
                     /locus_tag="PWZ48_003108"
     CDS             <1..>815
                     /locus_tag="PWZ48_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HDL2262670.1"
                     /translation="EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPD
                     KYSKIKQEITAIVKESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKR
                     YKSYKGTVGKIAKNVLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIIS
                     YSISTSPTYKLIEEMLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMS
                     RKGNCLDNSVIENFFGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLK"
BASE COUNT          355 a          104 c          132 g          224 t
ORIGIN      
        1 gagcaagaag aaatcaaggt taaaacaatc cttcagctca aaaaagaatt taaattgaat
       61 ctattacttt caattgcaca attagcgaag tcaacttatt attactgggt aaaaaaatta
      121 gataagccag ataaatatag caagataaag caagaaatta cagcgattgt aaaagaatca
      181 agaaactctt atggttatcg tagagtcact ttagcgttaa agatgaaagg atatacaatc
      241 aatcataaaa cagttagaaa attaatgtcc caaatgggac ttacctgtca aatccgaata
      301 aagcgatata aatcttataa aggaacagta ggaaaaattg ccaagaatgt gttaaaacga
      361 aatttttcag tagatacacc caataaaaaa tgggtgacag acgtcactga attcaagata
      421 aaaggaagaa aaatctatct atctcctata cttgatttat ttaacggaga aataattagt
      481 tatagtatat caactagtcc aacatataaa ctgattgaag aaatgcttca acaagcaatt
      541 aaaaaaaagg gaactgaggg ttcactgatt ctacattcag atcaaggatg gcagtatcag
      601 atgccacaat atcaaaaaaa attaaaagag aataacatta ttcaaagcat gtctagaaaa
      661 ggaaattgct tagataattc tgtaatagaa aatttttttg gtgtgcttaa atcagagttt
      721 ttttatcgag aaaaattccg atcaattgag atatttcaaa gtaaattaaa tgagtatatt
      781 aggtggtata ataacaaaag gataaaacta aagtt
//
LOCUS       DANKEF010000229          806 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000229 DANKEF010000000
VERSION     DANKEF010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.251"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..693
                     /locus_tag="PWZ48_003109"
     CDS             <1..693
                     /locus_tag="PWZ48_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262671.1"
                     /translation="ATISRHSSGKYYISLLCKEEIIELPKTNSTIGIDLGIMDFAILS
                     DGQKIDNNKFTSKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEK
                     VMNQRTDFLNKLSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYK
                     ADWYGRKIIKVDKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINIL
                     TEGLRLHSMGLA"
BASE COUNT          296 a          127 c          148 g          235 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tactatattt ctttgttgtg taaagaagaa
       61 attatcgaat tacctaaaac taattccaca attggtattg acttaggtat tatggacttt
      121 gcgattcttt ctgatggaca aaaaattgat aataataaat tcacgtccaa aatggaaaag
      181 aaactaaagc gtgaacaacg caaattgtct aggcgtgctt tattggctaa aaataaaggt
      241 accaatctat ttgaagctaa aaattatcaa aaacaaaaac gcaaagtagc tagattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aatgatcaaa
      361 aatcacgata ttatctgtat tgaagactta aacacaaaag gtatgttgcg aaatcataaa
      421 ttagctaaaa gtatttctga tgtttcttgg tcaaagtttg tgactaaatt acaatacaag
      481 gctgactggt atggacgaaa aatcatcaaa gtagataaat ggtttccgtc tagtcagatt
      541 tgttcagagt gcggacataa agatggcaag aaatctcttg aaattcgaga atggacttgt
      601 cctgtttgtc atactcatca tgatcgtgat atcaatgcta gtatcaatat tctgaccgaa
      661 ggtctaagac tacattctat gggattggct tagatccaat catcaagaac cgtaggaact
      721 acggggatag cttggtaaat aagagacacc gctgttagta aagaaatacg ctatcaagta
      781 tgctctattc ccaagaagct cccact
//
LOCUS       DANKEF010000230          792 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000230 DANKEF010000000
VERSION     DANKEF010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.252"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..781
                     /locus_tag="PWZ48_003110"
     CDS             <1..781
                     /locus_tag="PWZ48_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2262672.1"
                     /translation="DMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNG
                     VSLARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGE
                     LISLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKL
                     IKNNKDKYIFISKNGNPISIQAYNQSLRAVSKELNIDKKVSSHMLRHSHISLLTELGI
                     PLKAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
BASE COUNT          339 a          107 c          102 g          244 t
ORIGIN      
        1 agatatgcta gattactact attttgaaaa aaatttatca tataattatg taatacattt
       61 aaaaactttt acaggaatga tttttaaatt cgccaaccgt cgatatggat taaaatataa
      121 tcccgtaaat ggggtttctt tagcaagaaa acctaagaca aacgaagaaa taaaaaaaga
      181 aaaagatggt tatttatcaa aagaagaagt tcaaatgatt gctagtaaac aaaaaagttc
      241 acatcaacag tcacgttatt ctttaattac taaatttttg tttttaactg gactacgtta
      301 tggtgaattg atatctttga ctgaatctga ttatgatggt aataaaatta cggtttctgg
      361 tacttatgat tacgagttaa aaattaaaac aacaactaaa aatacaggtt cttatagaac
      421 cattgaatta tcaagtaatg ctaaagaaat catagatcaa ttaattgaag aaaacaaatt
      481 aattaagaat aataaagata aatacatttt catctctaaa aatggtaacc cgatatctat
      541 tcaagcatat aatcaatctt taagagcagt atccaaagaa ttaaacatag ataaaaaagt
      601 ttcttctcac atgttaaggc attcccacat ttctctactt acagaattag gaatcccatt
      661 aaaagccatt atggatcgtg taggacatga agattcaaaa acaacattaa agatttatac
      721 tcatacaact aaaaatatgc aaaatcaact agtagaaaaa ctaggaaaaa tagaaattta
      781 aattgcccct tt
//
LOCUS       DANKEF010000231          761 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000231 DANKEF010000000
VERSION     DANKEF010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..761
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.256"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            656..>761
                     /locus_tag="PWZ48_003111"
     rRNA            656..>761
                     /locus_tag="PWZ48_003111"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          313 a          124 c          134 g          190 t
ORIGIN      
        1 ccttaggcag cttttgctgt gtagaaagga ataaataata acgaaagttc aaaagctaat
       61 gccgactatt ctagacatta aagaagacga aaacgaacga taaagaaaga aaagttatga
      121 ataatcgttt attgaaaaaa acttgaaaaa atacaaaaaa ttcgttgacc aagctaaatt
      181 tagttggtat attattactt gtccaaaaga cagaagaaaa tatttattca aaaacataac
      241 aaaaaagtta ttgacatgtt actaacgaca tgataagata actgagtcgt taaaaaagaa
      301 ccacttgcga agcttgattc tatcgaaaaa aacttcaaaa aagttcttga caaataacaa
      361 acgataagtt atgataaatg agttgccgaa agatttcgaa aaacgaaatc gaaaaaaact
      421 tttaaaaaag agttgacaat cgcttgtctc tctgatatga tataaaagtt gctacggcag
      481 ccaaaaatga atcgggaaac actctcgatg aagtagacct ttgaaaactg aacaaagtaa
      541 gacaaaccaa atgtgtaggg cgtcttgatt caattcaaga caacaaacat ttttaacaag
      601 caagcaatat gctagcaaac aaattgagct taacaatcgc aagattgttc gaacttttta
      661 tgagagtttg atcctggctc aggacgaacg ctggcggcgt gcctaataca tgcaagtcga
      721 acgcttcttt ttccaccgga gcttgctcca ccggaaaaag a
//
LOCUS       DANKEF010000232          731 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000232 DANKEF010000000
VERSION     DANKEF010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.257"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PWZ48_003112"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="PWZ48_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDL2262673.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="PWZ48_003113"
     CDS             complement(481..675)
                     /locus_tag="PWZ48_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262674.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          220 a          156 c          122 g          233 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag t
//
LOCUS       DANKEF010000233          729 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000233 DANKEF010000000
VERSION     DANKEF010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 729)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..729
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.258"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..413
                     /locus_tag="PWZ48_003114"
     CDS             <1..413
                     /locus_tag="PWZ48_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262675.1"
                     /translation="QSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYG
                     EAVTGDNGKVRVDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNV
                     LVRWNLVAHRLGYEDIRLKEDTAYDSTVLDQKRF"
     gene            437..>729
                     /locus_tag="PWZ48_003115"
     CDS             437..>729
                     /locus_tag="PWZ48_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262676.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEY"
BASE COUNT          255 a          115 c          152 g          207 t
ORIGIN      
        1 aacaatcaac ggtacaagta tataaagatt ttactgttag aggtactaaa aactcaactg
       61 taccaacaga acattatgga caacgactat tgaacgctta tgaaactcca gaatattatt
      121 tcgctgatta tggggaagcc gttacaggtg acaatggtaa agttcgtgtt gatattgacc
      181 ccatgtttgc tgaaacagta aatctaagtc ggtatatgac acatgtgaca cctacagaac
      241 tagttttgtg tgctgttact catgaagata ttgaccattt catcattgaa actagtaagc
      301 caaacgtatt agttagatgg aatttagtgg cacaccgtct agggtatgaa gatattagat
      361 taaaagagga tacagcatat gatagcacag tgcttgacca aaaacgtttt taaaacgaag
      421 acaaggaggt atataaatgg ctagcagttt atataatttg gctttagatt tcagcaaaga
      481 attaaactac accaaagcta ttatggctcg tcaaggtgat aaagggatta cggtgacggt
      541 taaaccgttt ctaaatggct tgcagatgga tacgagtggc ggaacattta ctttaaaagg
      601 aacaacacca tctaaccgtt acgtagataa tgttgcaact agtgtaacta gtgaagaagt
      661 cacgttttct cttgatggca catttatgag tgaagcagga tattataaac actgctacgt
      721 agaatatag
//
LOCUS       DANKEF010000234          683 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000234 DANKEF010000000
VERSION     DANKEF010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.259"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            83..683
                     /locus_tag="PWZ48_003116"
                     /pseudo
     CDS             83..683
                     /locus_tag="PWZ48_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179228.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          259 a          117 c          114 g          193 t
ORIGIN      
        1 tccatgttcc ccactcctca agtgttttct ttagtatacg cctataggag gaaaaacgaa
       61 tacaatttaa ctcaatttat ataataggaa aaatttagcc gaatctgaag gaattagtag
      121 agttactcta tatcaatgga aaaacacggt tcttggaaag gattttccat tgcgtatgac
      181 tttaaaaaat caggaaaaac aaaaagagct acttttaaag gaagtagaag acttacaaaa
      241 acaagtacac caattacagc tcgaaaaagc attactcgaa ggagcggctg aactgctaaa
      301 aaaagaaaaa ggcgtcaatc ttctttgtct atcaaatcaa gaaaaaacga tattgattga
      361 tgccttacga aatcagttca ccttgaaaga gcttcttcaa cagctccagt tgcctaaaag
      421 cagctatttt tatcaaaaac aagcacttga gaaaccagat aagtattaca aagaacgtca
      481 attaattatc acgattttca atcataactt ctgtgcgtat ggttaccgta gaattgacca
      541 agctttaaaa aatatgggta aaaagctttc tgaaaaagtt gtacgtagat tgatgactga
      601 ggaaaatcta tttgttaaat tttctagacg aaaaaagtat tcttcgtatg ctggggaaat
      661 ttctcctgca caccccaatt tat
//
LOCUS       DANKEF010000235          679 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000235 DANKEF010000000
VERSION     DANKEF010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.260"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
BASE COUNT          240 a          114 c           80 g          245 t
ORIGIN      
        1 ccaaattact tcatcaaaac attgatataa tttttattat atcagtattt attcttgata
       61 cataaatgag aaaataaata ctttaaatct attgactaca agtagtcatt aagtcttcat
      121 aagatgtaca atatgacatt aaaaaaataa aagcttacga ccatccgtta aatctgacac
      181 taaataatgg actgctttct tttgtattag caggttacgt tgactatgaa tattcaccag
      241 attatttgga gaaacctctt caacacgaga acaatgatat taattttaga tttgtctacg
      301 agtcaaaaag taaaacatgg ttagttgatc aattaagcac gataaacaca agcttccatg
      361 aattacctaa acctgaaaat gaagttgaga tggtcattaa aatcttctaa aaaagaagcg
      421 ttgaagggga aatattcctt tcaacgctct tttttatatg cttagaaaca taaataaatt
      481 gtatactttc tttttagccc ctattacttt tcacgaaaaa tatctttttt tcgacgctag
      541 cgtttctcta cttctaatac tgtacacagt ttttgtatta ttgaaaaatt actcattatt
      601 tagcattaat ccacatcgtt ttatcaatcg accttcatac taaactactt taaagtcatc
      661 ttccttctcg tttttcatt
//
LOCUS       DANKEF010000236          677 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000236 DANKEF010000000
VERSION     DANKEF010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.261"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            119..397
                     /locus_tag="PWZ48_003117"
     CDS             119..397
                     /locus_tag="PWZ48_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262677.1"
                     /translation="MKEEQTTNKNYRSKTLKDIENEIKKQRAFLVPYTQERIQTTIDL
                     QVKKLHQELLDQRNKEQKKVLIQRSLDFLAPLCCLVLTLFAFWLVWGG"
     gene            399..>677
                     /locus_tag="PWZ48_003118"
     CDS             399..>677
                     /locus_tag="PWZ48_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262678.1"
                     /translation="MAFYLPYLLIFVSISGSIWLIYKIFQTRYSLKGSKIRFKRFFFL
                     GCIFSLIIVSSGLFGVLEGNKRVSRSILLGNVTQKYESARDKKKKEQAL"
BASE COUNT          239 a          122 c          127 g          189 t
ORIGIN      
        1 ctacgaagag ctcgagctag cattcgcgaa actgcagaag aagtacgaag agaacaacga
       61 actcttacgc ctgaacaacc ggaaattaga ccggctcgaa gaaaccatga acaacacgtt
      121 gaaagaggaa cagaccacca acaagaatta tcgctctaaa acgttaaaag acatagaaaa
      181 cgaaataaag aaacaacgtg cttttctcgt tccttatacg caagaaagga tacaaactac
      241 gattgattta caagttaaaa aactccatca agagctatta gaccagcgaa ataaggaaca
      301 gaaaaaggtg cttattcaac gatcattgga ttttcttgcc cccttatgtt gcttagtcct
      361 gacactgttc gcattttggt tagtatgggg aggctaaaat ggcattttat ttaccctatc
      421 tactcatttt tgttagtatt tcaggaagta tttggctcat ctataaaatt tttcaaacaa
      481 gatattcatt aaaaggctct aaaattcgat ttaagcgatt tttctttctt ggctgtatat
      541 tttccttgat aatcgtttca agcggcttat tcggcgtttt agagggaaat aagcgcgttt
      601 ctagaagtat tttattagga aatgtcactc aaaagtatga atcagcgcgt gataaaaaga
      661 aaaaagagca agcatta
//
LOCUS       DANKEF010000237          649 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000237 DANKEF010000000
VERSION     DANKEF010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.262"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>649
                     /locus_tag="PWZ48_003119"
     CDS             <1..>649
                     /locus_tag="PWZ48_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2262679.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSV"
BASE COUNT          213 a          111 c          134 g          191 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtag
//
LOCUS       DANKEF010000238          639 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000238 DANKEF010000000
VERSION     DANKEF010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.263"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..282
                     /locus_tag="PWZ48_003120"
     CDS             <1..282
                     /locus_tag="PWZ48_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDL2262680.1"
                     /translation="LIQDDLHHTHRLWLMNKINLYFGHSYVFAFYDYRTNITEVDLVI
                     SNYYIDTGKTPLLLMKNIPTERNWRLFEKTIYQLKKEKEVVKSAFCPEY"
BASE COUNT          218 a           89 c          119 g          213 t
ORIGIN      
        1 ttaatacaag atgatttaca ccatacgcat cggttatggt taatgaataa aattaatctg
       61 tattttgggc attcttatgt ttttgctttt tatgattaca ggacaaatat aactgaagta
      121 gatttggtta tttctaatta ctacatcgac actggaaaaa cgccattact gttaatgaaa
      181 aatataccta ccgaaagaaa ctggcgtttg ttcgagaaaa cgatttatca attgaaaaaa
      241 gaaaaagagg ttgtgaaatc agcgttttgt cctgagtatt agagaaacaa acggaaaaat
      301 agtttttcat attttgccgt ttttgagcta ataccgaggg acagctgatt gaacaccatt
      361 tattgagaga ttgtgaaaaa actgtcctgc atcaagtaat aagaacagcc aagtaaaaac
      421 agcttctcat gtttgttgaa tattgtgact tgtgaccgag gagttggcaa gagaacaccg
      481 tttattcgga aaaagggact gtgacactac atgtcacagt ccctttttga ttgaattatc
      541 tcatttgttt tctaattttt tctttatttg agaaaatagt tgaactggat aaaaagtact
      601 ggacgtgtca aaattatttt ggatcaaatt agatctatt
//
LOCUS       DANKEF010000239          620 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000239 DANKEF010000000
VERSION     DANKEF010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 620)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 620)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..620
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.264"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..496
                     /locus_tag="PWZ48_003121"
     CDS             <1..496
                     /locus_tag="PWZ48_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2262681.1"
                     /translation="DGETEMQTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFN
                     GLKQDGRIKPGIYSYGPDSVGEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIEN
                     GADEVAALELETKVMQDEFGKFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTA
                     TQSQ"
BASE COUNT          237 a           92 c          130 g          161 t
ORIGIN      
        1 agatggcgaa acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc
       61 aaaatctaaa ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta
      121 tcgacgtttc aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg
      181 tcctgattcc gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga
      241 aaatagaaag ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat
      301 tgaaaatggt gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga
      361 atttggtaaa ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca
      421 atggatgacg aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc
      481 cactcaatca cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg
      541 aactaaacga tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga
      601 tgatcaatag agattttaac
//
LOCUS       DANKEF010000240          617 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000240 DANKEF010000000
VERSION     DANKEF010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..617
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.265"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>617
                     /locus_tag="PWZ48_003122"
     CDS             <1..>617
                     /locus_tag="PWZ48_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262682.1"
                     /translation="ATISRHSSGKYYISLLCKEEVRELPKSNSAVGIDLGIIDFAILS
                     TGQKFDNNKFTSKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEK
                     VMNQRTDFLNKLSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYK
                     ADWYGRKIIKVDKWFPSSQICSNCGHKDGKKSLEIRKWTCPICHT"
BASE COUNT          226 a          101 c          107 g          183 t
ORIGIN      
        1 gctacaatat cacgtcattc tagcggtaaa tattatattt ctttgttatg caaagaagaa
       61 gttcgcgaac tacctaaatc taattctgct gttggtattg acctaggtat tatagacttt
      121 gcaattcttt ctaccggtca aaaatttgat aataataaat ttacgtccaa aatggaaaag
      181 aaactcaaac gtgaacaacg taagttgtca agacgtgcct tattagctaa acaaaaaggt
      241 atcaatctgt ttgaagctag aaattatcaa acacaaaaac gtaaagtagc acgattgcat
      301 gaaaaagtaa tgaatcaacg tactgatttt ctgaataagt tgagtacaga aattatcaaa
      361 aatcacgata tcatctgtat tgaagactta aacacaaaag gcatgttgcg taatcataaa
      421 ctagcaaaaa gtatttcgga tgtttcttgg tctagttttg tgtctaaatt acaatacaag
      481 gctgactggt acggtcggaa aatcatcaaa gtagataagt ggtttccatc tagccaaatc
      541 tgttcgaatt gtggacataa agacggcaag aaatctctcg aaattcgaaa atggacttgc
      601 cctatttgtc atactca
//
LOCUS       DANKEF010000241          580 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.266, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000241 DANKEF010000000
VERSION     DANKEF010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..580
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.266"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..506)
                     /locus_tag="PWZ48_003123"
     CDS             complement(<1..506)
                     /locus_tag="PWZ48_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262683.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQAILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
BASE COUNT          155 a          119 c          113 g          193 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcgc ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccgtcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagtcaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaat cgatccacag
      361 tgttcacaat gggaaggtga gtcatccaat ctccctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtagggtga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa
//
LOCUS       DANKEF010000242          574 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000242 DANKEF010000000
VERSION     DANKEF010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.267"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
BASE COUNT          185 a           81 c           81 g          227 t
ORIGIN      
        1 ttttttcttg atgtgatatc cttgtctttt ttgtaatgtt tttaacttat tgcttttgca
       61 aaatttattt acctgttttc tggatcaacg atagtgtaca ttatgctatc cttgtttgga
      121 ttttaggacg tttaaagagg cactttttta cataacttaa tccatagacc ttttaagcat
      181 gctgtgagat aataataaag aagagtttaa agcgcacccc aaaccacttc cccataagtg
      241 tattacgctt taaactcttt tatatttgaa ttcactaaga agcataccat atttttgaaa
      301 aaaagtgaga aaaaaggctt ataattagga tttagagtaa ttaattagat gtttatttta
      361 taggactgat actatagatt acaaatagta tcgtatttcg caatcttaaa attatcttct
      421 tactgtgaat aagataataa aatttgtatt tgtccttttt aggtaactta ttttaatcta
      481 tatacctttc tggatcaacg aaagtatact ttatatagtc ataacgccga tggtaactat
      541 gcactgtaac gataatgctg ctaaaatatc tttt
//
LOCUS       DANKEF010000243          559 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000243 DANKEF010000000
VERSION     DANKEF010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.268"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            75..>559
                     /locus_tag="PWZ48_003124"
     CDS             75..>559
                     /locus_tag="PWZ48_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HDL2262684.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     F"
BASE COUNT          209 a          103 c           90 g          157 t
ORIGIN      
        1 tccatacaaa aaaggaagtc ccttctgtag aataaagtta acgacaacca attcacagaa
       61 aagaggactt ccctatgaat gattttacta cagaaattgt gcaaactcta gtcactaaag
      121 gcgatttaaa tgaattattc cgttcgcact tagaaaaagc gataaacaca ctcctacgga
      181 ctgaattaac ggctttttta gattacgaaa aatatgatcg cactggtttt aattcaggta
      241 attcgagaaa cggttcttac tttcgatcaa tcaaaaccga atatggtgaa ttaacattgg
      301 aaatacctag agatcgtaat ggtgagttta aacaacaaac tttaccagcc tacaaaagaa
      361 caaacgatac attggaaacc actattatcc atttattcga aaaaggtgtt acgatgtctg
      421 aaattgctga tttgatcgaa aaaatgtacg gtcatcacta tactccacaa accatgtcca
      481 acatgactaa agttctgact gaagaagtaa atgcctttaa atccagagcc ttaaatgata
      541 agtatgtcgc tatttttat
//
LOCUS       DANKEF010000244          548 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000244 DANKEF010000000
VERSION     DANKEF010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.270"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..>548)
                     /locus_tag="PWZ48_003125"
     CDS             complement(<1..>548)
                     /locus_tag="PWZ48_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HDL2262685.1"
                     /translation="SLLYNSGAITELGSVDKGTTRTDNTLLERQRGITIQTGITSFQW
                     ENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAKDGVQAQTRILFHALRKMGIPT
                     IFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVELYPNMCVTNFTESEQWDTVIEG
                     NDDLLEKYMSGKSLEALELEQE"
BASE COUNT          147 a          119 c           84 g          198 t
ORIGIN      
        1 tcctcttgtt cgagttccaa tgcttctaat gatttaccgg acatatattt ctctaaaagg
       61 tcatcgtttc cctctattac cgtatcccat tgttcagatt cggtaaagtt cgtcacacac
      121 atattaggat acagttctac cttctgtttg attacaattt cggcagaaag tttctcttta
      181 atatcctgat aaaccgttga taaatcaatt ccattttggt caatcttatt gataaaaaag
      241 attgtgggaa tccccatttt cctaagtgca tgaaataata tacgagtttg tgcttgtacg
      301 ccatcttttg cagaaatcag tagaattgcc ccatctaaaa ctgataatga acgatatact
      361 tctgctaaga aatccatatg tcctggcgtg tctatgatgt tcaccttcgt attttcccac
      421 tgaaaagagg ttattcctgt ctgaattgta attcctctct gacgttctaa aagcgtatta
      481 tccgtcctcg ttgtaccttt gtccacgctt cctaattctg taatcgctcc actgttatat
      541 aataagct
//
LOCUS       DANKEF010000245          540 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000245 DANKEF010000000
VERSION     DANKEF010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.271"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            1..540
                     /locus_tag="PWZ48_003126"
                     /pseudo
     CDS             1..540
                     /locus_tag="PWZ48_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341471.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          152 a          136 c          133 g          119 t
ORIGIN      
        1 gtgccaattg gacattggcg attgtgtgtg acctgattct ccgggaatgg agttatggat
       61 atacgcaaaa aggcgtggcg gacctccttc accggctagg actaagctgg acccgtccga
      121 cgtataccct caaaaaagcg gattccaaga aacaacaggt atttttggaa gaaaccttcc
      181 cgagcctaaa aaaaaattgc tgaatagcga tattgaccac ctcctattcg aggacgaaac
      241 catgatccgg gattaccaag ccattggctg cacctggttt ctccgcggcc accagcgaaa
      301 aatccccact tatggacaac acaaaggggt aaaattgata ggcaccttgg attatgaaac
      361 cggtcggatc atttgtacag aagaaggggc atacgacgca gcggccttcc tccgttttct
      421 ggagcggacg ttgcgggaat accccaccgg aaaaatcgtg atggtattgg ataatgccct
      481 tatccaccac gccaagctga tccagtcttt tcttgaagaa aacgctaggc gcctggcact
//
LOCUS       DANKEF010000246          528 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000246 DANKEF010000000
VERSION     DANKEF010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..528
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.272"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(5..90)
                     /locus_tag="PWZ48_003127"
     tRNA            complement(5..90)
                     /locus_tag="PWZ48_003127"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(54..56),aa:Leu,seq:taa)
     gene            complement(107..178)
                     /locus_tag="PWZ48_003128"
     tRNA            complement(107..178)
                     /locus_tag="PWZ48_003128"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(144..146),aa:Gly,seq:gcc)
     gene            complement(190..262)
                     /locus_tag="PWZ48_003129"
     tRNA            complement(190..262)
                     /locus_tag="PWZ48_003129"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(227..229),aa:Thr,seq:tgt)
     gene            complement(280..361)
                     /locus_tag="PWZ48_003130"
     tRNA            complement(280..361)
                     /locus_tag="PWZ48_003130"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(325..327),aa:Leu,seq:tag)
     gene            complement(369..441)
                     /locus_tag="PWZ48_003131"
     tRNA            complement(369..441)
                     /locus_tag="PWZ48_003131"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(406..408),aa:Lys,seq:ttt)
     gene            complement(445..517)
                     /locus_tag="PWZ48_003132"
     tRNA            complement(445..517)
                     /locus_tag="PWZ48_003132"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Val,seq:tac)
BASE COUNT          135 a          152 c          128 g          113 t
ORIGIN      
        1 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
       61 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      121 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      181 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      241 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      301 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      361 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      421 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      481 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DANKEF010000247          517 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000247 DANKEF010000000
VERSION     DANKEF010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.273"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
BASE COUNT          169 a           87 c          117 g          144 t
ORIGIN      
        1 tatccataaa actaatataa agcttggtac gattctgttt gtaagacata cgaatagtat
       61 cagaggtagg agccaagctg cctgatatta aattatcgat aaagtagaaa ggttaagcat
      121 tagccgctgc aataactaat gctaggagac agcaacgtgt aaccattacc gcacgtgttt
      181 ctgaatggtg gtagcgaccg atttgaacgg ctagggcgga aatacggtgc aaatgcacgg
      241 tggtttcatg caatgcttac agggcgtatt ttgtaagggt atgtaacgga gataattccg
      301 acggtaatgg atgtgagagg ccaagccgac gctgacggta gggcaaacaa acatagttgc
      361 actctagcga tcgttattta tttttaacgg tcaaactagg gtacaactat gcttaaaaca
      421 cttcgcctca acttctggta gcaaaagtga ttttgaaagt caaaatattt tttatgagtt
      481 atttacaatt ctaaaaaaca agtgtattaa tctacta
//
LOCUS       DANKEF010000248          513 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000248 DANKEF010000000
VERSION     DANKEF010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.274"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(107..>513)
                     /locus_tag="PWZ48_003133"
     CDS             complement(107..>513)
                     /locus_tag="PWZ48_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HDL2262686.1"
                     /translation="TPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMNA
                     ASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYEDS
                     QITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          162 a          102 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttc
//
LOCUS       DANKEF010000249          510 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000249 DANKEF010000000
VERSION     DANKEF010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.275"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..434)
                     /locus_tag="PWZ48_003134"
     CDS             complement(<1..434)
                     /locus_tag="PWZ48_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262687.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
BASE COUNT          146 a           85 c           80 g          199 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc
//
LOCUS       DANKEF010000250          500 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000250 DANKEF010000000
VERSION     DANKEF010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..500
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.276"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>500
                     /locus_tag="PWZ48_003135"
     CDS             <1..>500
                     /locus_tag="PWZ48_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein"
                     /protein_id="HDL2262688.1"
                     /translation="SLYPVYHGSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFK
                     IEYSEERQRLAYVRLYGGILHLRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEI
                     VILQNEFLKLNSVLGDTKLLPQRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDS
                     DPLLQY"
BASE COUNT          179 a           82 c          100 g          139 t
ORIGIN      
        1 tccttgtacc ctgtttatca tggaagcgca aaaagcaaca tagggattga gcagcttata
       61 gaagtgataa cgaataaatt ttattcatca acatacagaa agaagtctga actttgcgga
      121 aatgtcttca aaattgaata ttcggaagaa agacaacgtc ttgcatatgt acgcctttat
      181 ggcggaatcc tgcatttgcg ggattcggtt agaatatcgg aaaaggaaaa aataaaaatt
      241 acagaaatgt atacttcaat aaatggtgaa ttatgtaaaa ttgataaggc ttattccggg
      301 gaaattgtta ttttgcaaaa tgagtttttg aagctaaata gtgttcttgg agatacaaag
      361 ctattgccac agagagagag aattgaaaat ccgctccctc tgctgcaaac aactgttgaa
      421 ccgagcaaac ctcaacaaag ggaaatgtta cttgatgcac ttttagaaat ctccgacagt
      481 gacccgcttc tacaatatta
//
LOCUS       DANKEF010000251          486 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000251 DANKEF010000000
VERSION     DANKEF010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..486
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.277"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>486
                     /locus_tag="PWZ48_003136"
     CDS             <1..>486
                     /locus_tag="PWZ48_003136"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262689.1"
                     /translation="TMEAIAQVASEVTEVQPAEAGVPAGEATVEQAPVTEAPTVNSKT
                     TLDQTIGETQHPGNSPGTSQPVEETASITTPASETSTLEGEKAAEATPSVQPESSEKQ
                     AEEKKRIEETVEQPAATADKEGKNEENVEKTISSTAASQNQPTKPNKESTASIENQKK
                     K"
BASE COUNT          185 a          119 c          107 g           75 t
ORIGIN      
        1 ctacgatgga agccattgcc caagtcgctt ctgaagtgac agaagtccaa ccagcagaag
       61 ccggggtccc agctggtgaa gccactgttg aacaggcccc tgtgacagaa gcgccaacgg
      121 tcaattccaa aacgactctc gatcagacga ttggggaaac acaacaccca gggaatagtc
      181 ctggtacttc acaaccagtt gaagaaactg cctcaattac aacccctgct tcagaaacgt
      241 ccactctaga aggagaaaaa gctgccgaag caacgccttc tgtgcagcca gagtcttctg
      301 agaaacaagc ggaagaaaag aaacgaatag aagaaacagt ggaacaacca gcggctactg
      361 ctgacaaaga aggaaaaaat gaggaaaacg ttgaaaaaac aatctcttca accgcagcta
      421 gtcaaaatca accaacgaaa ccaaacaaag aatctacggc tagtattgaa aatcaaaaga
      481 aaaaag
//
LOCUS       DANKEF010000252          470 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000252 DANKEF010000000
VERSION     DANKEF010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..470
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.279"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>470
                     /locus_tag="PWZ48_003137"
     CDS             <1..>470
                     /locus_tag="PWZ48_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
                     /protein_id="HDL2262690.1"
                     /translation="ALSKRGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKT
                     TNVLNRKFLRDNPLEAIVTDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLV
                     KKAFSRIKQPLTEVEIFHTDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAV"
BASE COUNT          167 a           78 c           88 g          137 t
ORIGIN      
        1 aagccttatc aaaacgaggc attcagatca gccgacgaaa aattagtaga atcatgaaaa
       61 atagaggatt aaaatcgagc tatactgttg cttattttaa agtacatcat tctacttgca
      121 atgaagccaa aacgacaaac gtattgaatc gtaaattctt aagagacaac ccattagaag
      181 cgatcgtaac agacttgact tatgtacgag tcgggaaaaa atggaattat gtctgtttca
      241 ttttggatct gttcaatcga gaaattctcg gctattcttg tggagaacat aaagatgccg
      301 ttctagtaaa aaaagcattt agccgtatca aacaacctct gacagaggtt gagatttttc
      361 atactgatcg tggaaaagag tttgataacc aagctattga tgaattatta acaacttttg
      421 acatcaatcg atcattgagt cataaaggct gtccttttga taatgccgta
//
LOCUS       DANKEF010000253          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000253 DANKEF010000000
VERSION     DANKEF010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.280"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..430)
                     /locus_tag="PWZ48_003138"
     CDS             complement(<1..430)
                     /locus_tag="PWZ48_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2262691.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRA"
BASE COUNT          132 a           92 c           59 g          179 t
ORIGIN      
        1 ctgctcttaa acgaataact tcttcttcaa gagactcatc tttaagtttt ttaggaatgt
       61 ttagcttgga attcatacta atttttctgc cccttttttg gctctcaagt gaagaagcac
      121 cgccttcttc atattgctct atccatttac ttagagttct atttgaaccg atattaaatt
      181 ttttagcagt ttcttggata gaaagaccat ttgtttccat atattctata acatcaagtt
      241 taaattttgt agtgtagctt ttgccacctc caaccaagcc ttcccaacca tgatagttat
      301 aaatccttac ccaatgtcta accagtgtac gatttatttg atatttatgt gcaagatatt
      361 tgtagccgcc ttcgttattt aaatagtctg aaactacttt tttcttaaaa acaaatgtat
      421 atttccgcaa aaaaagcacc ccttttaatt agatttctag tc
//
LOCUS       DANKEF010000254          462 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000254 DANKEF010000000
VERSION     DANKEF010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.281"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            152..412
                     /locus_tag="PWZ48_003139"
                     /pseudo
     CDS             152..412
                     /locus_tag="PWZ48_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
BASE COUNT          178 a           70 c           93 g          121 t
ORIGIN      
        1 taaatttgga aagttacacg ttactaaagg gaatggagat aaattattag atatactact
       61 gacagcttcc aagaagctaa agaggtccct agcgcctacg gggaatttgt atcgataagg
      121 ggtacaaatt cccactaagc gctcgggacc ccttgtagga aaatgtccta agtgtggcaa
      181 caatattgta ttaaaaaaat cgttttatgg ttgttcaaat tatcctgaat gtaagtttac
      241 tttagctgaa cattttagaa agaaaaaact aaccaaaacg aatgtaaaag aattactgga
      301 gggaaaagaa accctggtaa aaggaatcaa aaacaaagag aaaaagccct acaatgccgt
      361 tgtaaaaatt ggggaaaagg gatatattga ttttatctct ttttcaaaat aaacatgggg
      421 ctgacccaaa agtagctcct atatgaaaaa tagaggtctt tc
//
LOCUS       DANKEF010000255          460 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000255 DANKEF010000000
VERSION     DANKEF010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.282"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>460
                     /locus_tag="PWZ48_003140"
     CDS             <1..>460
                     /locus_tag="PWZ48_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HDL2262692.1"
                     /translation="YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKEN
                     VKKETTKTKLKDNNNLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQV
                     ATITDKTTLAARANAIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQR"
BASE COUNT          180 a           84 c           62 g          134 t
ORIGIN      
        1 attataagct cttttatgaa tggcttctga gtgagtctat cgttcctgat acttctatca
       61 cctcgataag acagttaact gcctatgacc taaacttgta caaatctcat ttgaaacgtc
      121 gcacaaaaga aaatgtaaaa aaagagacaa caaaaactaa gctcaaagat aataataact
      181 taggtctatc tacttctact atcaatagaa atatcacggc attaaaggtt ctatttaaat
      241 atctttcgaa aagttccaat aatcctctag gaaaaccata cttagacaat aatcctatgg
      301 atcaagtagc aaccatcaca gataaaacta cgttagctgc acgtgccaat gccattgaga
      361 aaaaattatt tctagataag gatacacaaa attatctaga ttatattgcc aatgaatata
      421 aaaatacttt gagtaaacga gctttgatct attatcaacg
//
LOCUS       DANKEF010000256          454 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000256 DANKEF010000000
VERSION     DANKEF010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..454
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.283"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
BASE COUNT          205 a           62 c           56 g          131 t
ORIGIN      
        1 gcgcttatga agcgctacca aaaatttacc atattttaca aaaaataaca agtcttaaaa
       61 aaatgacaga taaaaatgca caaagaatta gatatagata accgcgaaaa atcaagcttt
      121 agaaagttgc ttttagctag accaattaaa aatcacaaag tgagcgtctc tatccattgg
      181 tagaggggct acttggcata gtgtacatta tgtcaactat gcaattaaat aactaactac
      241 ttcatattag cttaataaaa taatcaaaat aactaggggg aaaaacattg tataaaaaaa
      301 tttttcattt tttaaataag aatagtaaat ataaaaataa tttaagatat ctattactat
      361 taatccagat agagcaaacc aagtaaatat tccatgtgta aagctaaaaa aattcatttg
      421 aatcaattca aataaaccta atatagtaac aaat
//
LOCUS       DANKEF010000257          450 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000257 DANKEF010000000
VERSION     DANKEF010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.284"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>450
                     /locus_tag="PWZ48_003141"
     CDS             <1..>450
                     /locus_tag="PWZ48_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2262693.1"
                     /translation="SGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISGIFDNLNISIPHIPLPEFIMEGSF
                     NPLKGQIPHLRVKWHAKGGIFTKPTLLGGMNGVGEAGPEAVLPLKRSV"
BASE COUNT          152 a           75 c           92 g          131 t
ORIGIN      
        1 agcggaattt ggaacggtat caaaaatact gctttgaatc tttggaatgg aattacaagc
       61 actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg gtccaatcta
      121 acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa catatgggaa
      181 ggcatcaaaa agactgttag cgctccaatt gattggatta gagacaaaat cagtggcatt
      241 tttgataatt tgaatatttc tataccacat attccgttac cggaatttat catggaaggc
      301 agctttaacc cgctaaaagg tcaaatcccc catttgcgtg taaaatggca tgctaaagga
      361 ggtatcttta ctaaaccaac tttactaggt ggaatgaacg gtgtcggtga agcaggacct
      421 gaagcagttt tacctttgaa aagatctgtt
//
LOCUS       DANKEF010000258          443 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000258 DANKEF010000000
VERSION     DANKEF010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.285"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            81..431
                     /locus_tag="PWZ48_003142"
     CDS             81..431
                     /locus_tag="PWZ48_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HDL2262694.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          182 a           48 c           72 g          141 t
ORIGIN      
        1 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
       61 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      121 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      181 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      241 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      301 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      361 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      421 ctcaacgata aaatatagta tac
//
LOCUS       DANKEF010000259          438 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000259 DANKEF010000000
VERSION     DANKEF010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..438
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.286"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>438
                     /locus_tag="PWZ48_003143"
     CDS             <1..>438
                     /locus_tag="PWZ48_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDL2262695.1"
                     /translation="SGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSV"
BASE COUNT          145 a           67 c           93 g          133 t
ORIGIN      
        1 agcggaattt ggaacggtat caaaaacact gctttgaatc tttggaatgg aattacaagc
       61 actattagct ctaaagtaaa cgatggaaaa aatgcaattt caagcggttg gtccaatcta
      121 acaggtattg tttccgacat attcaataat gttaaaagta caattgctaa catttgggaa
      181 ggtatcaaaa agactgttag cgctccaatt gattggatca gagataaaat cagtagtatc
      241 tttgataatt tgaatatttc tataccacat attccgttac cacattttaa attgagcgga
      301 gaattcaatc cattgaaggg gaaaatccca acgttgggtg ttgattggta tgcgaaaggt
      361 agtgtgttta attctccgaa tattatcggt gtcggcgaag caggacctga agcagtttta
      421 cctttgaaaa gatctgtg
//
LOCUS       DANKEF010000260          437 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000260 DANKEF010000000
VERSION     DANKEF010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.287"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            150..>437
                     /locus_tag="PWZ48_003144"
     CDS             150..>437
                     /locus_tag="PWZ48_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2262696.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTY"
BASE COUNT          157 a           88 c           73 g          119 t
ORIGIN      
        1 ttcttcttca agaccaaata aaaaggctct ataatttcta ggactgatag aaaatcattc
       61 tatcggtccc ttttgagcat gaaaaaacaa aacatacaga agctccgtta gaattgaatc
      121 accacaacac aatcaaagga gcgttctgta tgcctgatct tcatcatatc aaactactac
      181 ttggaatcaa agataaaaac attttcatca caaatgtgga atcgaagagc attaagaata
      241 tcaagagtct tgttgtttct gcgacgttaa gtaaagaaat tcgtcgctgt cctttgtgta
      301 aacagatgaa tcatgaaggc atgatcgtta agaacggaaa gaaaaaatcg ctcattcaac
      361 ttaataaatg tgcgaaccaa ctgacttatc ttgctttagc gaaacaacgc taccactgtc
      421 gtggctgcca cacctat
//
LOCUS       DANKEF010000261          414 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000261 DANKEF010000000
VERSION     DANKEF010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.288"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>414
                     /locus_tag="PWZ48_003145"
     CDS             <1..>414
                     /locus_tag="PWZ48_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2262697.1"
                     /translation="QIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSI
                     QQLLKLPAECFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKN
                     QFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFNGR"
BASE COUNT          141 a           78 c           70 g          125 t
ORIGIN      
        1 acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct gacatctatc tgattgttga
       61 agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc tcttgcacac
      121 tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc ctaaaccaaa
      181 agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag ataaatattg
      241 gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc aactgtttac
      301 taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa gtaccgttac
      361 ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga ggaa
//
LOCUS       DANKEF010000262          406 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000262 DANKEF010000000
VERSION     DANKEF010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.289"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>406
                     /locus_tag="PWZ48_003146"
     CDS             <1..>406
                     /locus_tag="PWZ48_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262698.1"
                     /translation="FICVDGTTNQLFTILEDRRLYKLTQYFLRFPRKARLKVKYLVMD
                     MNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQIMNRFRTSHSENQKKYRRL
                     KRYWKLLLKDSTTLEPLKRHYHRLFKRPISQTE"
BASE COUNT          128 a           96 c           71 g          111 t
ORIGIN      
        1 tttatttgtg ttgatgggac aaccaatcag ctatttacga tccttgaaga tcgcagactt
       61 tacaaactaa ctcaatactt cctgcgcttt cctagaaagg cccgtttaaa ggtcaagtac
      121 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
      181 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      241 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      301 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccactcaaa
      361 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaa
//
LOCUS       DANKEF010000263          404 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.290, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000263 DANKEF010000000
VERSION     DANKEF010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.290"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            1..346
                     /locus_tag="PWZ48_003147"
                     /pseudo
     CDS             1..346
                     /locus_tag="PWZ48_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          143 a           77 c           80 g          104 t
ORIGIN      
        1 agcggtgctg actgacggtt tcctccgatt ttctatgcac tatggttttg agacacaggt
       61 gtgcaatcca cgtagcggaa acgagaaagg tagtatggaa aacaaagtcg gctatgtacg
      121 gtacaacttc ttttcatcta caccgattat gaaggatttc gccagtttca acgaggcatt
      181 agcggtggag ctgtcacacg atcgagagcg aatccattac gaaaaacatg tcattatcaa
      241 tgatctatgg aacacagaaa aaacagattt gctagctttg ccggacaccg attacccagt
      301 atttaaagaa atcgaagtca aagccaataa gtacaacgaa attaaacaaa acaaaataac
      361 aaatttatcg tagttcaata tacttgatag aaaaactatt tatt
//
LOCUS       DANKEF010000264          397 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000264 DANKEF010000000
VERSION     DANKEF010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..397
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.292"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..378
                     /locus_tag="PWZ48_003148"
     CDS             <1..378
                     /locus_tag="PWZ48_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262699.1"
                     /translation="EICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIENQ
                     DLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYNQ
                     KFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          150 a           71 c           63 g          113 t
ORIGIN      
        1 gaaatctgtg atgactttaa ggctgtttat caagctaact caaaagaaga agcgaatacc
       61 ttcttatccg gcatgattga gaaatggaag aaaaactatc ctaaagtgac gcagtcactc
      121 atagaaaacc aagacttatt aactttttat gattttccac ctagcattcg tagaaccatt
      181 tactcaacca atctaatcga gtctttcaat aagcaaatta aaagatacag ccgtagaaaa
      241 gagcagtttc aaaatgaaga atcactagaa cgctttctag tcagcatttt tgatacatac
      301 aatcaaaaat ttctaaacag aagccataaa ggttttcaac aggtaaccga tacattagtt
      361 tcaatgttta ctgagtaact aattattttg caggagg
//
LOCUS       DANKEF010000265          386 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000265 DANKEF010000000
VERSION     DANKEF010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.293"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(102..>386)
                     /locus_tag="PWZ48_003149"
     CDS             complement(102..>386)
                     /locus_tag="PWZ48_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HDL2262700.1"
                     /translation="QEQFNPGLDMIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQ
                     NIIKRSNKVSTWSKNGITEHKGYDKKVLSMYENVFFEMLERIIQLENEKE"
BASE COUNT          104 a           72 c           49 g          161 t
ORIGIN      
        1 ccacaatcac tcacttcttt ctatttcttc ttattcttat tttatcatca acaatcacaa
       61 atcacaagtg atttgtgatt aatcacttgt gatttgtgat tctattcttt ttcgttttct
      121 aattgaataa ttcgctcaag catttcaaaa aatacgttct catacatgga taaaactttt
      181 ttgtcatagc ctttgtgttc tgtaattcca tttttagacc aggtacttac tttattactt
      241 cgcttgataa tattttggaa aaccaagtta tcttctttat gttgcttgta cagttcttcc
      301 aggtttgatt ttatcgttgc gctgtccgta tcaactaaat aaggaacaaa accgatcata
      361 tctagtccag ggttaaattg ttcttg
//
LOCUS       DANKEF010000266          386 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000266 DANKEF010000000
VERSION     DANKEF010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.294"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>386
                     /locus_tag="PWZ48_003150"
     CDS             <1..>386
                     /locus_tag="PWZ48_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262701.1"
                     /translation="EQLELKKREQDLAEQQMAIEKQRLNQEKEKEAAARKERQEQMDT
                     LRQAQLNYLAQPQPTPSVAAVQTPMNNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGT
                     VFAFNHINQSSQADSVATTTSIKEEA"
BASE COUNT          144 a           84 c           75 g           83 t
ORIGIN      
        1 gaacaattag aactaaaaaa acgtgaacag gatcttgcgg aacaacaaat ggcaatagaa
       61 aaacaacggt taaaccaaga aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa
      121 caaatggata cattgcgaca agctcagtta aattatctag cacaacctca accaacgcct
      181 tcagtagctg ctgttcaaac cccaatgaac aaccaaacaa atctaactcc ttcaccacag
      241 cgttccttta acggatggat ggtgggatgt attgcaagtt taagcttgct tctaggtgga
      301 ggaacagtat ttgcttttaa tcatatcaat caatcttcac aggctgattc tgtggccaca
      361 acgacgtcaa tcaaggaaga agccca
//
LOCUS       DANKEF010000267          380 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.295, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000267 DANKEF010000000
VERSION     DANKEF010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..380
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.295"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>380
                     /locus_tag="PWZ48_003151"
     CDS             <1..>380
                     /locus_tag="PWZ48_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDL2262702.1"
                     /translation="SGKTSLRSAISEETQGNVVIIDNDTFKQQHPNFDELVKLYEKDV
                     VKYVTPYSNRMTEAIISRLRDKGYNLVIEGTGRTTDVPIQTATMLQAKDYETKMYVMA
                     VPKINSYLGTIERYETMYADDPMTA"
BASE COUNT          145 a           66 c           72 g           97 t
ORIGIN      
        1 tcaggtaaaa ctagtttgcg atcggcaatt tccgaagaga cacaaggaaa tgttgttatc
       61 attgataatg atacgttcaa acaacagcac cctaattttg atgaactagt gaaactttat
      121 gaaaaagacg tagtaaaata cgttacccct tattctaatc gcatgacaga agcgatcata
      181 agccgtttga gagataaagg gtataattta gtgattgaag gtacaggacg aacaacagac
      241 gttcctattc aaaccgcaac aatgcttcaa gccaaagatt atgaaacaaa aatgtatgtc
      301 atggcagtac ctaaaatcaa ctcatattta ggaacaattg aacggtatga aactatgtat
      361 gcagatgatc caatgacagc
//
LOCUS       DANKEF010000268          377 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000268 DANKEF010000000
VERSION     DANKEF010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.296"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>377
                     /locus_tag="PWZ48_003152"
     CDS             <1..>377
                     /locus_tag="PWZ48_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262703.1"
                     /translation="EQLEIRKREQDLAEQKMAIEKQRLEQEKEKEAAARKERQEQMDT
                     LRQAQLNYLAQPQPVAAVQTPVNNQANLTPSPQRSFNGWMVGCIASLSLLLGGGTVFA
                     FNHINQSSQADSVATTASIKEEA"
BASE COUNT          137 a           76 c           83 g           81 t
ORIGIN      
        1 gaacaattag aaataagaaa acgggaacag gatcttgcgg aacaaaaaat ggcgatagaa
       61 aaacaacggt tagagcaaga aaaggaaaaa gaagcagctg ctcgtaagga acgtcaagaa
      121 caaatggata cattgcgtca agcacagttg aattatctag cacaacctca accagtagct
      181 gctgttcaaa cgccagtgaa caaccaagca aatttaactc cttcacccca gcgttccttt
      241 aacggatgga tggtgggatg tattgcaagt ttaagcttgc ttctaggtgg aggaacagta
      301 tttgctttta atcatatcaa tcaatcttca caggctgatt ctgtagccac aacggcgtca
      361 atcaaggaag aagccca
//
LOCUS       DANKEF010000269          375 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000269 DANKEF010000000
VERSION     DANKEF010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.297"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..132)
                     /locus_tag="PWZ48_003153"
     CDS             complement(<1..132)
                     /locus_tag="PWZ48_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HDL2262704.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGASFIR
                     "
BASE COUNT          132 a           69 c           76 g           98 t
ORIGIN      
        1 tcgtatgaat gaagcacctt cgatgtcatt ggcaacagag atttcagata agatagacaa
       61 ttgattgtcg tctgttaaaa aacaaacatc tagtttttca gagctaacgt caataccgac
      121 aaataatttc atggaagaag gacctccttt cgatataaat tttggaaaat gcttcgatac
      181 tgtgggattt cccagaaact cactgtatag ccacaacctc gcctaataga atacggtgat
      241 aaaagagcta gaagctgcct tccaaactac tatttgaaag tctagtccat tatcgaaaca
      301 gctaatgagt tggtagtgat aacagtggtt cgggtaacag actaaacagg gtagacaaac
      361 gctacaggag gaaat
//
LOCUS       DANKEF010000270          369 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000270 DANKEF010000000
VERSION     DANKEF010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 369)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 369)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..369
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.298"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            122..>369
                     /locus_tag="PWZ48_003154"
     CDS             122..>369
                     /locus_tag="PWZ48_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDL2262705.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNG"
BASE COUNT          124 a           74 c           74 g           97 t
ORIGIN      
        1 tttatataat aaaatgcaat acttttcgag agtgtaaaat attttgtgta aatagaaaaa
       61 aggaagtccc ttctgtagaa tagagttacc acaacacatt cacagaaaag aggacttcca
      121 tatgaacgat tttactacag aaattctaaa gactctagcg aacaaaggcg atttgaatga
      181 attattccgt gtccatttgg aaaaagctgt caatacgctt ctcaaaacgg agttaacggc
      241 tttcctcgat tacgaaaagt acgatcgcat tggttttaac acggggaatt ctcgtaacgg
      301 ctcctatgac cgtacggtca agaccgagta cggggaactt catctccaga ttccgcgcga
      361 ccgcaacgg
//
LOCUS       DANKEF010000271          368 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000271 DANKEF010000000
VERSION     DANKEF010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 368)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 368)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..368
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.299"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            76..>368
                     /locus_tag="PWZ48_003155"
     CDS             76..>368
                     /locus_tag="PWZ48_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HDL2262706.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRT"
BASE COUNT          131 a           62 c           76 g           99 t
ORIGIN      
        1 ggtattaaaa aacatatcgt tctctagtat gattaaatca cgacaaaata aacaactgag
       61 aggacgatat gctatatgaa tgattctatc aaaaaaatgc tgcgattaat agagaaagat
      121 ttgatgatta cagaggtctc ttacgagacc tttcagaaga aaaagacgtt gatcgtcgat
      181 gctgttttct cacctgctcc ccatacctgt agaaattgtg gttctactgt ggtagatgga
      241 aacgggaaag taattgtagt gaaaaatggg aagaaggaaa cgattgtccg ctttgaacaa
      301 tacaatcaca tgcctttggt tatgcgctta aaaaagcagc gctatacctg taaaaactgt
      361 cgaaccca
//
LOCUS       DANKEF010000272          332 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000272 DANKEF010000000
VERSION     DANKEF010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.300"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>332
                     /locus_tag="PWZ48_003156"
     CDS             <1..>332
                     /locus_tag="PWZ48_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262707.1"
                     /translation="IILSFLGKVQMEVISALLQEKYHVEIELKEPTVIYMERPLKNAE
                     YTIHIEVPPNPFWASIGLSVSPLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQ
                     GLYGWNVT"
BASE COUNT          103 a           53 c           78 g           98 t
ORIGIN      
        1 attatacttt ctttcttagg gaaagtacaa atggaagtga ttagtgcact gttgcaagaa
       61 aagtatcatg tggagataga actaaaagag cctacagtca tttatatgga gagaccgtta
      121 aaaaatgcag aatataccat tcacatcgaa gtgccgccaa atcctttctg ggcttccatt
      181 ggtttatctg tatcaccgct tccgttggga agtggaatgc agtatgagag ctcggtttct
      241 cttggatact taaatcaatc atttcaaaat gcagttatgg aagggatacg ctatggttgc
      301 gaacaaggat tatatggttg gaatgtgacg ga
//
LOCUS       DANKEF010000273          328 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000273 DANKEF010000000
VERSION     DANKEF010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.301"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..>328)
                     /locus_tag="PWZ48_003157"
     CDS             complement(<1..>328)
                     /locus_tag="PWZ48_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HDL2262708.1"
                     /translation="RAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYV
                     IHAFLDGLSFYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFI
                     YFFVFTF"
BASE COUNT          141 a           60 c           44 g           83 t
ORIGIN      
        1 agaatgtaaa aacaaagaaa tatataaatg cccatgcaat accaaaagga attaatttta
       61 accaattagt tttgtcattc ccttgaagta caccgaataa taaaaagtcg atcaaaccac
      121 ctgaaaagga attccctaca cgaatattta agatatcagc aaaataaaaa gataatccat
      181 ctaagaatgc atgaatcaca taaagccaag gagcaacaaa taaaaacata tattcaactg
      241 gttcagtaat tccagttaaa aatgatgtaa gggccccgct aaaataaagc ccaccattct
      301 ttttacgatt tttcttagga attgcacg
//
LOCUS       DANKEF010000274          323 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000274 DANKEF010000000
VERSION     DANKEF010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.302"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PWZ48_003158"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="PWZ48_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HDL2262709.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT          100 a           49 c           51 g          123 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgttatatc aactcatttc ttg
//
LOCUS       DANKEF010000275          321 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000275 DANKEF010000000
VERSION     DANKEF010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.303"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(69..321)
                     /locus_tag="PWZ48_003159"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="PWZ48_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DANKEF010000276          312 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.304, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000276 DANKEF010000000
VERSION     DANKEF010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..312
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.304"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>312
                     /locus_tag="PWZ48_003160"
     CDS             <1..>312
                     /locus_tag="PWZ48_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262710.1"
                     /translation="GLYYSPVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAP
                     QEYLSRAYNDAPKYCANIVDTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLT
                     EL"
BASE COUNT           97 a           55 c           60 g          100 t
ORIGIN      
        1 ggcttatact atagccctgt tagtacccca gcagattttc ggatgcttgc tcctattgta
       61 ttggaacaag tcttaaaaaa agctggaaca gaattgttag agccatatct tagttttaaa
      121 atttatgcgc cacaggaata tctttcacga gcatacaacg atgctcctaa atattgtgcg
      181 aacatcgtag acactcaatt gaaaaataat gaggtcattc ttagtggaga aatccctgct
      241 cggtgtattc aagaatatcg tagtgattta actttcttta caaatggacg tagtgtttgt
      301 ttaacagagt ta
//
LOCUS       DANKEF010000277          308 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000277 DANKEF010000000
VERSION     DANKEF010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.305"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>308
                     /locus_tag="PWZ48_003161"
     CDS             <1..>308
                     /locus_tag="PWZ48_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HDL2262711.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     "
BASE COUNT           91 a           56 c           64 g           97 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttag
//
LOCUS       DANKEF010000278          306 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000278 DANKEF010000000
VERSION     DANKEF010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.306"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>306
                     /locus_tag="PWZ48_003162"
     CDS             <1..>306
                     /locus_tag="PWZ48_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6-like element IS1216 family transposase"
                     /protein_id="HDL2262712.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYRSLRTASTTIKGMEAIRGLYKKT
                     "
BASE COUNT          110 a           60 c           59 g           77 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
      241 aagtttacgc actgcctcta ccacgattaa aggcatggaa gccattcgag gattatataa
      301 gaaaac
//
LOCUS       DANKEF010000279          299 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.307, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000279 DANKEF010000000
VERSION     DANKEF010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..299
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.307"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>299
                     /locus_tag="PWZ48_003163"
     CDS             <1..>299
                     /locus_tag="PWZ48_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HDL2262713.1"
                     /translation="QETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKES
                     SGSYTATNGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEA"
BASE COUNT          102 a           70 c           64 g           63 t
ORIGIN      
        1 caagaaacgg ttcaaacgga aacacaagca gcgcctgttg ctgaaacaca acctgctcct
       61 gctgtaacag aaactgctgc tactccagca agtacaagct ctgcaaaaga atggatcgca
      121 caaaaagaat caagcggttc ttacacagca acgaacggac gttacatcgg ccgttaccaa
      181 ttagattctt cttacttaaa tggtgactac tctgctgcaa accaagaaag agtagcagaa
      241 caatatgtta cttcacgtta tggttcttgg gaagcggcga aagcattctg ggaagcaaa
//
LOCUS       DANKEF010000280          298 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.308, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000280 DANKEF010000000
VERSION     DANKEF010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..298
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.308"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            18..>298
                     /locus_tag="PWZ48_003164"
     CDS             18..>298
                     /locus_tag="PWZ48_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein ImpB"
                     /protein_id="HDL2262714.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYI"
BASE COUNT          104 a           49 c           54 g           91 t
ORIGIN      
        1 agaatggagt gttatttatg atggattatt caaaagagcc agtaaatgat tactttttaa
       61 tcgatatgaa aagcttttac gcaagtgtcg aatgtataga aagaaattta gatccattaa
      121 caacagaact tgttgttatg agtcgagctg ataatactgg ttcaggattg atattagctt
      181 cttctcctga agcaaaaaag cggtatggta ttacaaatgt gagtagacca cgtgacttac
      241 cacaaccatt tcctaaaaca ctacacgttg ttccaccacg tatgaaccta tatatcaa
//
LOCUS       DANKEF010000281          295 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.309, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000281 DANKEF010000000
VERSION     DANKEF010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.309"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            76..>295
                     /locus_tag="PWZ48_003165"
     CDS             76..>295
                     /locus_tag="PWZ48_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HDL2262715.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFP"
BASE COUNT          111 a           57 c           51 g           76 t
ORIGIN      
        1 agacaaataa aaaagccatt tatgttacac tcaaattgta atacccgcta aagcatacaa
       61 ggagagtgaa cataaatgac ttatacccat cttacaccaa acgagcttgt aatgatagaa
      121 gcatattttc atcaagaaac tccggttgct atcgttgcga agcagcttaa acgtggacgc
      181 caaacaattt acaatgtcta taactttctc aaatgtggtg gaacagcact tgaatacttt
      241 gaacaataca aagaaaataa gcgacgttgt gggagaaccg aaatcatttt tcctg
//
LOCUS       DANKEF010000282          292 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.310, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000282 DANKEF010000000
VERSION     DANKEF010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.310"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..185
                     /locus_tag="PWZ48_003166"
     CDS             <1..185
                     /locus_tag="PWZ48_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDL2262716.1"
                     /translation="YTFETLQQLDLELFDYVNWWNHLRLHGTLGYETPVGYRNQRLAQ
                     RILDNELGCANASEAV"
BASE COUNT           83 a           63 c           69 g           77 t
ORIGIN      
        1 aatacacatt tgaaacctta caacaattgg atttggagtt atttgactat gtcaattggt
       61 ggaaccacct tcggttgcac ggtacacttg gctacgagac accggttggt taccgtaacc
      121 agagattggc gcagcgaatc cttgataatg agctcggatg tgctaacgct agcgaggcag
      181 tctaacttta actgttagct cctgccgaag atcgtcacat ccgaggaggc tcattgtcaa
      241 ggacaatcgg aatagcatac ggaaagaagt tctgcacctt ataaaatttg tc
//
LOCUS       DANKEF010000283          290 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.311, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000283 DANKEF010000000
VERSION     DANKEF010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..290
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.311"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            37..>290
                     /locus_tag="PWZ48_003167"
     CDS             37..>290
                     /locus_tag="PWZ48_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDL2262717.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
BASE COUNT           88 a           53 c           57 g           92 t
ORIGIN      
        1 atagatttat gaaaacgaat gcaaggagtg atagctatga tgtctaaaaa caatacgaat
       61 ggtagaaatc aatttgcgat gcttacgatt gatgatctag ttcctcaaga tcatctcgtt
      121 cgaaagattg atgccgcgct tgattttgaa tttatttatc caatcgtcga agccacttac
      181 tccgatttag gtcgcccaag tatcgatcca gtgattctga tcaaattggt gtttattcag
      241 tatttattcg gtatccgttc catgcgacaa accattaagg aagtcgatac
//
LOCUS       DANKEF010000284          275 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.312, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000284 DANKEF010000000
VERSION     DANKEF010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.312"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            94..>275
                     /locus_tag="PWZ48_003168"
     CDS             94..>275
                     /locus_tag="PWZ48_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HDL2262718.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT           84 a           57 c           44 g           90 t
ORIGIN      
        1 tgcttctttt acctatgaaa tattttcacg tgttaaagta ttcctcgttg aacacaacaa
       61 ataaaacttt ttaacgaata aaggagattt ttcatgacat cacttaaaac tttacttttt
      121 ggaacaactt tggctgccgg cgctgcattc ttcatgggaa cgactgctca tgcggacgaa
      181 gcttataccg ttcaatcagg tgatactcta tcaacgattt ctcaaaaata cgttggtgat
      241 aactccttga tcaatgccat tgcagaatca aattc
//
LOCUS       DANKEF010000285          265 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.denovo.313, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000285 DANKEF010000000
VERSION     DANKEF010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 265)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 265)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..265
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.denovo.313"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..>265
                     /locus_tag="PWZ48_003169"
     CDS             <1..>265
                     /locus_tag="PWZ48_003169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDL2262719.1"
                     /translation="KAGFVTEDNLYLDSTHIKANANKHKFTKEMTHDEAKAYQDELED
                     EINRQRIEAGKRPFTLDLEKEVKLKERKISKADPESGYYVKGER"
BASE COUNT          106 a           44 c           61 g           54 t
ORIGIN      
        1 aaagcaggtt ttgtaacaga ggataatttg tatctggatt ctacccacat caaagctaac
       61 gccaataaac ataagtttac gaaagaaatg acccatgatg aagcgaaagc ctatcaggat
      121 gaattagaag atgagatcaa tcgtcaacga atagaggctg gaaaacgccc ttttactttg
      181 gacttagaaa aggaagtgaa gctgaaagaa agaaaaataa gtaaagctga tccggaaagc
      241 ggctactacg tcaaaggcga acgag
//
LOCUS       DANKEF010000286         8102 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.089, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000286 DANKEF010000000
VERSION     DANKEF010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.089"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(134..577)
                     /locus_tag="PWZ48_003170"
     CDS             complement(134..577)
                     /locus_tag="PWZ48_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HDL2262720.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="PWZ48_003171"
     CDS             complement(795..2135)
                     /locus_tag="PWZ48_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDL2262721.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="PWZ48_003172"
     CDS             2404..3300
                     /locus_tag="PWZ48_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HDL2262722.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="PWZ48_003173"
     CDS             complement(3450..4391)
                     /locus_tag="PWZ48_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDL2262723.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="PWZ48_003174"
     CDS             4517..5638
                     /locus_tag="PWZ48_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HDL2262724.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWZ48_003175"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="PWZ48_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HDL2262725.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="PWZ48_003176"
     CDS             7426..7992
                     /locus_tag="PWZ48_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDL2262726.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2687 a         1511 c         1600 g         2304 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aa
//
LOCUS       DANKEF010000287         6518 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.104, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000287 DANKEF010000000
VERSION     DANKEF010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6518
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.104"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            267..1535
                     /locus_tag="PWZ48_003177"
     CDS             267..1535
                     /locus_tag="PWZ48_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HDL2262727.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            2022..2486
                     /locus_tag="PWZ48_003178"
     CDS             2022..2486
                     /locus_tag="PWZ48_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDL2262728.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIEH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            2470..3099
                     /locus_tag="PWZ48_003179"
     CDS             2470..3099
                     /locus_tag="PWZ48_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HDL2262729.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMQPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITTFE"
     gene            3453..4931
                     /gene="msr(C)"
                     /locus_tag="PWZ48_003180"
     CDS             3453..4931
                     /gene="msr(C)"
                     /locus_tag="PWZ48_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HDL2262730.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRPDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            4978..5424
                     /locus_tag="PWZ48_003181"
     CDS             4978..5424
                     /locus_tag="PWZ48_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HDL2262731.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(5623..6138)
                     /locus_tag="PWZ48_003182"
     CDS             complement(5623..6138)
                     /locus_tag="PWZ48_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HDL2262732.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            6255..>6518
                     /locus_tag="PWZ48_003183"
     CDS             6255..>6518
                     /locus_tag="PWZ48_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2262733.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKK"
BASE COUNT         2153 a         1125 c         1309 g         1931 t
ORIGIN      
        1 aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa tatccaaaga
       61 gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa tggagaagaa
      121 tattcttcat ttacgtcttt ttatgtagaa aaagcaaatt tatcaaaaag aaacaaatgt
      181 tatttaaata tggacattga cttgctttta cttttttagt tacactgtta tttaggaaaa
      241 gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt agcgttattc
      301 ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg acagaaactg
      361 tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca gagagtactt
      421 tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca actgaaacaa
      481 cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat ccagagaatg
      541 cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc catcaagcag
      601 atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca tctgctaatt
      661 ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca agtgccaatg
      721 acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg gggaacagta
      781 ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat aatggccaat
      841 ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg aatgccgctt
      901 ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta ttgaagacga
      961 cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat acgaactctt
     1021 acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat tatggttcta
     1081 cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca ccaacttcaa
     1141 cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat acattgaatg
     1201 ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt gcttggcaag
     1261 caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga gaccatcatt
     1321 acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt tatgaaggtc
     1381 taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca aatgaaaaaa
     1441 ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca ccgatgggct
     1501 ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa aagctgccct
     1561 gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg tttggttgaa
     1621 cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt gtgacaacat
     1681 cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa tattcgacgg
     1741 gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattttct actgcagaag
     1801 agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg tgctttcttt
     1861 atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc tattgagagc
     1921 gtttttttta ttacacagct gattctttga cttttttctt tttttattct attattgaca
     1981 agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta gaagagatta
     2041 gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc tttgaagaaa
     2101 atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa gaaaatccat
     2161 tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt actcgtcatc
     2221 tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag aacaaccggg
     2281 aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat cgagcattgt gaaatggacc
     2341 ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg aaacaatttc
     2401 taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa acgaaagagg
     2461 ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag aagatccgtt
     2521 cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat gatcaaagaa
     2581 gcgacgactg caccttcttc tgtcaatatg caaccatggc gctttgtggt cgctgaaagc
     2641 gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca aaatgatact
     2701 tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg cgaagaaatc
     2761 tataaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca acaattagct
     2821 gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt agtcaaagtc
     2881 gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg ctatgaaaca
     2941 aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt ggataaagac
     3001 cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg ttacgagtct
     3061 gttcgtttag caccagaaaa gatcactacg ttcgaataaa aggttttcag ttactattca
     3121 gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag gtcggaaaaa
     3181 tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata aattcaaaag
     3241 acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac gacagtagaa
     3301 taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc agagtaggaa
     3361 aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt atcgataatt
     3421 gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta aatataacaa
     3481 atctacaagt cagctttggc aaccagctag aactatctat tgattctctt cgtgtctatc
     3541 agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact ttgctcaaac
     3601 taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc accttcaact
     3661 acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa ttagccagtc
     3721 atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg gaagaacgaa
     3781 agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta gacgagccaa
     3841 caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt tattattatg
     3901 gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca tcgaaaatct
     3961 gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc tatctcactc
     4021 aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg aaagagaaaa
     4081 aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg gaaaaagtgt
     4141 ccagtaaaaa gaaaaagcaa caaatcagac cggatcggtt gtcttcctct aaacaaaaag
     4201 acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga ctccaaaaaa
     4261 taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta ccaaaatctc
     4321 ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa agaagtggga
     4381 gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc gcgattgtcg
     4441 gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa ggagaaggaa
     4501 tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat caaatgacat
     4561 ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa tcgcttgtcc
     4621 gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg ttatgtacat
     4681 taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag ccaagtaatg
     4741 tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa gctttagaga
     4801 agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct tactttgtcg
     4861 agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa gtacttacga
     4921 gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg gtacagattg
     4981 cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt gatggtcttg
     5041 gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag tttgttgatt
     5101 ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat cggtgtgctg
     5161 gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca aatgatctta
     5221 actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt cggtgtgatc
     5281 atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg gaaaatgaat
     5341 gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag aagtgtgcta
     5401 aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc cttggcatgc
     5461 tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac gataggtttt
     5521 ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat tgaagataat
     5581 ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa aaaactgctg
     5641 catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca taattctgcg
     5701 ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt cattcaattt
     5761 tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata gcagtccagt
     5821 agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt catctttttg
     5881 cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc cttcatcagt
     5941 caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca cataaccctg
     6001 ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc aaaaaattgt
     6061 gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc ataataatat
     6121 ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat tatatcacag
     6181 taaagtttcc acgttttaca tgtgcatatt tacatgtaaa ataaatcatg tatgatgcta
     6241 atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa caaacaaatg
     6301 aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg atcgctttta
     6361 tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta aacgctacgc
     6421 cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg gtgatgctat
     6481 ttacaggttt cttatctagt cggatcggtt tgaaaaaa
//
LOCUS       DANKEF010000288         5071 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.118, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000288 DANKEF010000000
VERSION     DANKEF010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5071
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.118"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            128..1168
                     /locus_tag="PWZ48_003184"
     CDS             128..1168
                     /locus_tag="PWZ48_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDL2262734.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
     gene            1266..1454
                     /locus_tag="PWZ48_003185"
     CDS             1266..1454
                     /locus_tag="PWZ48_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HDL2262735.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(1512..1865)
                     /locus_tag="PWZ48_003186"
     CDS             complement(1512..1865)
                     /locus_tag="PWZ48_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262736.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            2396..2818
                     /locus_tag="PWZ48_003187"
     CDS             2396..2818
                     /locus_tag="PWZ48_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HDL2262737.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            2815..3045
                     /locus_tag="PWZ48_003188"
     CDS             2815..3045
                     /locus_tag="PWZ48_003188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDL2262738.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            3543..3743
                     /locus_tag="PWZ48_003189"
     CDS             3543..3743
                     /locus_tag="PWZ48_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HDL2262739.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            3770..4963
                     /locus_tag="PWZ48_003190"
     CDS             3770..4963
                     /locus_tag="PWZ48_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDL2262740.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
BASE COUNT         1739 a          826 c          933 g         1573 t
ORIGIN      
        1 gtacattttg tcattttgta ctccaaaact gaactactta cttgcatata tacgccttta
       61 tagtggagta ctacatttac gagattcggt tagagtatca gaaaaagaaa aaataaaagt
      121 tacagaaatg tatacttcaa taaatggtga attatgtaag attgatagag cttattctgg
      181 agaaattgtt attttgcaaa atgagttttt gaagttaaat agtgttcttg gagatacaaa
      241 actattgcca cagagaaaaa agattgaaaa tccgcaccct ctactacaaa caactgttga
      301 accgagtaaa cctgaacaga gagaaatgtt gcttgatgcc cttttggaaa tctcagatag
      361 tgatccgctt ctacgatatt acgtggattc tacgacacat gaaattatac tttctttctt
      421 agggaaagta caaatggaag tgattagtgc actgttgcaa gaaaagtatc atgtggagat
      481 agaactaaaa gagcctacag tcatttatat ggagagaccg ttaaaaaatg cagaatatac
      541 cattcacatc gaagtgccgc caaatccttt ctgggcttcc attggtttat ctgtatcacc
      601 gcttccgttg ggaagtggaa tgcagtatga gagctcggtt tctcttggat acttaaatca
      661 atcatttcaa aatgcagtta tggaagggat acgctatggt tgcgaacaag gattatatgg
      721 ttggaatgtg acggattgta aaatctgttt taagtacggt ttatactata gccctgttag
      781 tactccagca gattttcgga tgcttactcc tattgtactg gagcaagcct ttagaaaagc
      841 tggaacagaa ttgttagagc catatcttag ttttaaagtt tatgcaccac aggaatatct
      901 ttcacgggca tataacgatg ctcccaaata ttgtgcaaat atcgtaaata ctcaactgaa
      961 aaataatgag gtcattatta ttggagaaat tcctgctcga tgtattcaag attatcgcaa
     1021 tgatttaact ttttttacaa atgggcttag tgtttgttta gcagagctaa aaggatatca
     1081 ggttaccact ggcgaacctg tttgccagac ccgtcgtcta aatagtcgga tagataaagt
     1141 aagatatatg ttcaataaaa taacttagtg cgttttatgt tgttatataa atatggtttc
     1201 ttattaaata agatgaaata ttctttaata tagatttgaa ttaaagtgga aaggaggaga
     1261 ttgttattat aaactacaag tggatattgt gtcctatttg tggaaataaa acaagactac
     1321 gaatacgagt ggatactata cttaaaaatt tccctttata ctgccccaaa tgtaagaacg
     1381 aaactttaat taatgttcaa aaaatgaata taataacaat caaagagcca gacgccaaga
     1441 cgcagagccg ataatttgag aaatgaaact ctcatcttat cggctctttt tgtttatctg
     1501 aattttactg actagccttc aatatttctt gcttcattga taccttttgc tagtgcttcc
     1561 attaaggata gttctttgtc tgtaaagcta tccatgtatt tttctacctg taaccttcga
     1621 gtgcttttaa tcaaattatc tgtaggtaag aaaaattcat caatagatac atggagtaat
     1681 gatacaagat catacagcac ttgtgtactt gggtgttgtc ctttgttttc tatatttgtt
     1741 aaataacgtg ggtcaatttc aatcattgtt cccacttgtt cacgagtgag accccgtttc
     1801 attcgagcct ctttaatcgc taaaccaacg gctctaaaat cgtatttatc ttctttttta
     1861 cgcatactaa atcacctcta tacattttac tgttcctata taaccataaa caggtataga
     1921 aaaacatgtt atatagttta tgagttccta tagaacactg tggaatttat taatgcgatg
     1981 aatccaattt caaatagaaa ggtcgctgat tttatttacc tatacaactt tataaaattt
     2041 agtcatactc ctagaaagta gataaaattg gcgacttaat ggagggaaaa gtcgccatga
     2101 gaaagataat ctgtcatcta gttctcttaa ttaagttctt atcaaaaaaa cttatccacc
     2161 tgtactggat agatatgacc atcaatgtgg ttttgcagat tacatagata gaaaaatcaa
     2221 agcatatgaa cagagaaaac aatctgttta tgtgcttttt tggtcactta ataaatatct
     2281 tttaggattt ctcttcattt atgcaacaaa tcccccgttc gtgtcggggt ttagtatgaa
     2341 gggagataaa gaagcacatg ccactccctt tcacgacaga aagggggtga aaagcatgaa
     2401 accatcttct tttcaagaaa ccatagaaaa tcagtttgac tatatctgca aacaagttat
     2461 tgaagatgaa cgaaaagatt acttcaaaca tctagacaga ctatcagcaa aagaagtctc
     2521 gttttctgat atagataact accttgtcaa ctctttttct actgttgata cgtacgtcac
     2581 tgatttccaa ctatttaaat tatttggatt aagaatcaat gttgaaagtg atttattaag
     2641 tgaagccctg cgtcatcttt cagagaaaaa acggaacatt attttacttc attactttat
     2701 ggatatgagt gatgtggaga ttgcggagtt actaaatttg aatcgttcga ctgtttatcg
     2761 tcatcgaata agtggactag ccatgattaa agaattcatg aaggagtgta aagaatgaaa
     2821 acagcttatc caagggttcc cttccccctc attgtaaagg cgacagatgg cgacgtggaa
     2881 gcaattaatc agattgttaa acattatcga gggtatacct caaaacgttc attacgtcgc
     2941 atgacagacg aatatggaaa ttctcatatg gttattgatg aaaccctacg tggcagaatg
     3001 gaaacgaaac tcatctctaa gattttatcg ttcgaaatta aatagtccca ttttttcgtg
     3061 gaagcgtgtc gaatcgtatc cacgcttccc gatttgggag gtttgttatt ccatcaaagc
     3121 atattgtgtg ttcagtaggt tttgataggc taacaagcca aacgttcttt gacaaccaaa
     3181 taaaggcaat cgaatacgtt tcggtaagaa aagagctgat gaactaacac gccatgacct
     3241 acaaaattag tgagcgattc atgttagtaa ttcgaaagta atctttagca ggattgctgc
     3301 catgactttc ttatcgtgat aatgatactc ccgtatagcc atagtccgag cgttagaagc
     3361 gtcacaggca atgggtacgg ctacatgaga accatgcaga agtgaaattc ctgtgagctt
     3421 tgctagagct gttcgattgc taagatattc ttctcttgtc agtgtattat tataaaacta
     3481 tttgatataa ttataggtga atattggagt tttcctagcc tataattggg aaaggagtaa
     3541 taatgataac ggtcacaaaa aaggatttag ttgagctagg atatggaact tctttttcag
     3601 cagacatcat aaaaaaagca aaagaattga tgattacaaa agggcatatc tactatcaat
     3661 ctcgaaagct agatagagtc cctcgtgaag ctgttgaaga aattctagga attcggtttt
     3721 caaatgaaaa caggtctgag tagtttttgc acagtaagga gtaaaaacta tggttaaaga
     3781 cccaataaaa aaagcaaaga atggaaccta ttattttcgt gcaaatctag gttatgattc
     3841 taaagggaaa aaaattcaaa aatatcgtag tggattcact actaaaaaag aagcaagaga
     3901 agaatattcg aaactgctat tgatgaaacc agaagaatta agtgagaata aggataaaat
     3961 gacgtttaaa cattatatct ttgaaatttt ccttccatgg tataagacac aggtaaagct
     4021 cagaacatat gaaaatcgct taccaactat taagaagcat ttctcttatt ttgataaaat
     4081 ggctgtttcc gatattgagc ctattgatgt gcaaaattgg caactaaaat tatcaaagaa
     4141 gtgtaagtct tcttatgtaa gagctgttca aggactattt tcagttgcta tggatcgagc
     4201 aattgtcctt gggataacca ccacaaatcc atctaagatt attggcaatg tgaaaaagca
     4261 aaagtctaaa attgaatttt ggacaaaaga agaatttgaa aaagtaatct ctctaattta
     4321 caaagaagat tactatcaac actttttatt tatctctctg tggtttttat ttatgactgg
     4381 aatgcgaatt ggtgaagcta ctgccattca atgggaagat attgattttg actcaggagt
     4441 actaaccatc aacaaaacac tctattacaa gaatcaatcg aattatagat ttgttgaacc
     4501 aaaaacaaag gcgagcgttc gtcatattgt attagataaa tgcacattaa cttatttatc
     4561 cgaatggaag gaagtacaac aaaatctaat tcagacagac tttgtaatga gctataacgg
     4621 tattcctacc caaaaacata cactatcata tgctattgaa cgctatgcta agctggctgg
     4681 tatccatcga atcagaatcc atgggttacg gcattctcat gcttccttgt taatcagtat
     4741 gggagaaaat ccacttatca ttaaagatcg tctaggacac gaagatattg agacgacttt
     4801 aggaacgtat ggacatcttt atcctaacag taatttcgag gtagctaaca aattaaatgg
     4861 agccatttcc tttaaagaag caaaacaaaa ttatgatact tcaccaaaga atcaatttac
     4921 tgtagactac ttgcgaaaca aagaaatgaa aaaagtgcaa taaaagtgca gtaaaaagat
     4981 aagaagagcc agaaaccctt ggtataagag gatttctagc tctcctacaa ctattcccac
     5041 tccactgttg ctggtggttt agaagtgatg t
//
LOCUS       DANKEF010000289         4117 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.130, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000289 DANKEF010000000
VERSION     DANKEF010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.130"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..242
                     /locus_tag="PWZ48_003191"
     CDS             <1..242
                     /locus_tag="PWZ48_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDL2262741.1"
                     /translation="FACNNAYCINEKKAIKLLETFEHKPEKYTEKVNHIFEVLGISLF
                     ECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI"
     gene            223..957
                     /locus_tag="PWZ48_003192"
     CDS             223..957
                     /locus_tag="PWZ48_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDL2262742.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            990..1898
                     /locus_tag="PWZ48_003193"
     CDS             990..1898
                     /locus_tag="PWZ48_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HDL2262743.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            1895..2437
                     /gene="sat4"
                     /locus_tag="PWZ48_003194"
     CDS             1895..2437
                     /gene="sat4"
                     /locus_tag="PWZ48_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HDL2262744.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            2530..3324
                     /locus_tag="PWZ48_003195"
                     /note="aph(3')-IIIa"
     CDS             2530..3324
                     /locus_tag="PWZ48_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HDL2262745.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            3600..>4117
                     /locus_tag="PWZ48_003196"
     CDS             3600..>4117
                     /locus_tag="PWZ48_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HDL2262746.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEP"
BASE COUNT         1413 a          626 c          945 g         1133 t
ORIGIN      
        1 tatttgcatg taataatgct tattgtatca acgaaaagaa agctataaaa ctgcttgaaa
       61 cttttgaaca taaacctgaa aaatataccg agaaggtaaa tcatattttt gaagtactcg
      121 gtatctcact ttttgaatgc tacgacatga ccgagaagct ttataatgaa gtgaatgaaa
      181 ttgtatcgga gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat
      241 gatgataata tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct
      301 ggtgcgggag aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg
      361 cttgatttag gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc
      421 tctgtagtag gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat
      481 tttccacaga ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc
      541 tttgatgtaa tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata
      601 aaaaagatat ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct
      661 gtttttactg ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat
      721 ttcccggtgg acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag
      781 gttacaaaat atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt
      841 ataataaatc agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg
      901 gcggatgaaa tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa
      961 tatagaaaaa ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag
     1021 tactttcttt agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg
     1081 caaatattaa tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg
     1141 atatagaacc gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga
     1201 tgcaaaagcc ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta
     1261 tgctatttga tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata
     1321 attacctaaa gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa
     1381 gggacatagt tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg
     1441 atgattgctg caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta
     1501 aggaaatttt atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa
     1561 tgatatcatg gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata
     1621 agtttattga aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga
     1681 tggattccta tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg
     1741 tatccggtga ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa
     1801 caaaatatac cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca
     1861 catatgccgc tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca
     1921 cctgaaagat atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag
     1981 gtatgaaaac gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag
     2041 ctaccaagac gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa
     2101 tactgataag ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact
     2161 gcgaaaaaat tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag
     2221 ggggcaaggc ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa
     2281 cttgcatgga ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca
     2341 taattgtggt ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa
     2401 ctttgaaaaa gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga
     2461 gttcgtcttg ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg
     2521 aaataataaa tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac
     2581 cgctgcgtaa aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa
     2641 aatgaaaacc tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg
     2701 gaacgggaaa aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg
     2761 cactttgaac ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt
     2821 tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag
     2881 tgcatcaggc tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac
     2941 agccgcttag ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa
     3001 aactgggaag aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg
     3061 gaaaagcccg aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt
     3121 gtgaaagatg gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag
     3181 tggtatgaca ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat
     3241 gtcgagctat tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat
     3301 attttactgg atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca
     3361 agctttttag acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt
     3421 ttatatcttt tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg
     3481 gtgaagaggt ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca
     3541 gctagcttaa gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt
     3601 tgaacaggat taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc
     3661 ctgccaaaga aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc
     3721 ggacacttga agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg
     3781 tagagggctt cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta
     3841 cagcagggaa atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag
     3901 ctatgtataa aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg
     3961 agaacagtat taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta
     4021 ttgcagcaat tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat
     4081 caggagggca agaaccggaa agcagaatga tagagcc
//
LOCUS       DANKEF010000290         3277 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.143, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000290 DANKEF010000000
VERSION     DANKEF010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.143"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            275..772
                     /gene="dfrG"
                     /locus_tag="PWZ48_003197"
     CDS             275..772
                     /gene="dfrG"
                     /locus_tag="PWZ48_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HDL2262747.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(844..2796)
                     /locus_tag="PWZ48_003198"
     CDS             complement(844..2796)
                     /locus_tag="PWZ48_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HDL2262748.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(2803..3039)
                     /locus_tag="PWZ48_003199"
     CDS             complement(2803..3039)
                     /locus_tag="PWZ48_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262749.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
BASE COUNT         1016 a          566 c          538 g         1157 t
ORIGIN      
        1 taagtagttc agttttggag tacaaaatga caaaatgtac ttcaaaatgg actacttttt
       61 tcattttaac ttacatacca aattacatac aaaatgattt ataaaatgca attgaaaatg
      121 ataattgaat gaaaatttgt gattttgtgg tattatgata atataactta tagaaaaaat
      181 tgcttattca actaacgggc aggataatga gaaaaggttt tttgatagaa ataagaaatt
      241 agatggtaaa ttagttaaaa aggctgtgtt ataaatgaaa gtttctttga ttgctgcgat
      301 ggataagaat agagtgattg gcaaagagaa tgacattcct tggaggattc ccaaggactg
      361 ggaatatgtt aaaaatacta caaagggaca tccgataata ttaggtagga agaaccttga
      421 atcaatcgga agagccttac ctgacagaag aaatattatt ctgacgagag ataaggggtt
      481 tacctttaat ggttgtgaaa ttgttcattc aatagaagat gtttttgagt tatgtaaaaa
      541 cgaagaagaa atttttattt tcggaggaga acagatttat aatttgtttt tcccttatgt
      601 tgagaaaatg tacatcacaa aaatacatca tgaattcgaa ggagatactt tttttccaga
      661 agtgaattat gaggaatgga atgaggtatt tgcccaaaaa gggataaaga atgataaaaa
      721 tccgtataac tactattttc atgtatatga aagaaaaaac ttattgagtt aacgggtgcg
      781 ttaattgaag aagaacagta aagataaagc acaagagctt gggattactc caagctcttt
      841 ttgtcatttg aaataaatta tatctttcca atcaaattgt ttatctgtaa tccctaatct
      901 ttgagcaggt gtcaaaacct ttttatccgc tgatttaaaa gggcgacaaa agttgtagta
      961 agttcttaga attgtaatcg caaattgagc atacttcgga ttgaagttag cgtaaatata
     1021 gctcttctta tcaccacgag ctgttgttaa tggtctttca agagatgata tataacgtct
     1081 tatctgttga ataaagctat tggtcgaatg gtcattaaca tttaatagca tctttgcgat
     1141 ttcgtttggt tccagagcag ataaatccgt tctacaatca actgaataaa atcctttatc
     1201 cttagtggct aatggatgtt tgatagggtt atcggcatat ttgcgatatc tttctccatc
     1261 tttactaata gcctcttcgt ggaattgatg acttgttagt aattcagtta gataatgaga
     1321 tgccaaagtc cgtagaaact ttgttttgaa tcctctgata tctccccaat cgagcagttc
     1381 tgcttttgct tgtccgaatt cttttaagca ttgttttctg cttttcgtct tgttaacttg
     1441 gcaaataaaa tgatgtgcat cgctcaacct taattctttc gtgaatactc tataaaaggc
     1501 gtttctaata gagaagtcat catcactaat cattcgccat tcgtcggcat taaccatttg
     1561 tttaattaac caatagtgag cagttgtagt ataaggagca tcaacattta aaccatcgac
     1621 aaatctactc ctattagtaa ttttccctaa ttcgtgaaga taagcattcc tattttcgct
     1681 atcgttagcg gatggttctt gtggataata agaccaatct aacctatcat ttttacgact
     1741 aaaagtattg agatggtctt ctttgaattt tcttgtatct tcatttaatt catccattga
     1801 aatattccaa tcataggcaa catcagaacg gaacacatat cttgataaga cctcagcaga
     1861 tacaactata tacgtttgca tattaaggtc ttcaaacccc gtatatttct ttgaaccttg
     1921 tcctttctta cggacgttat ttaggtagta gtgcatcttg tccgtattaa gccacatctc
     1981 gttaaatttc tttgtttgaa ggggttgtgt ctcatatcgt tctaaaaact ccaaacaacg
     2041 cctatacagc cactccagct tatgatagta ggttccaact ccaattccta aaatatcaca
     2101 agtacgactg actgaaaccc ttccaacaac catcttagcg aacattggaa gaatggtgtt
     2161 cttttgttgg tgataagtag tggtttcttc tctttttggt agaacattag tgaacttctt
     2221 acacgcctta cattgatagc gttgagattt gcccctactt ttcccccttt tgtaaaattg
     2281 cttcggctca ttgaatgggg ttgattcttc ttccgagcag ccttctttat gaaattgata
     2341 atctggctca acatcttgga tgctattgat ttctataagg cgtttaattt cttcaacaac
     2401 agaccaatta gataaagggg ttactgtaca gttttgcgat acacctctgc caataggatt
     2461 gtcattgcag tacatacctt tatgaccttt atctttggaa ctaccagtca ttttatatcg
     2521 acttggtttc ccttttacat ctttgaattt gtgctgttct ttcccaaaat tacaacaaaa
     2581 cggattgatg cagtaattat attgaatggg atattcagaa ccgttccatt taaaagacgt
     2641 tggcaagaac aacattttgt tataccttgt tgcaaatcgt tttggagtta ataaatcata
     2701 ttgtttagcc ctatctttta attcttcgtt tgaaatcgga attttaacat ctacaatttc
     2761 atcttccttc gtggctaatc ttttaagctt ggtcattgtc tatcaaccca ctctatcttt
     2821 gatacccatt ttttcaacaa tccgcttatt acttcgcttg ttggcaatcc attcaataaa
     2881 cttcttatcg tctaaaatgt tcaataagaa ttcatccact gcttcgcttt cagtacgctc
     2941 tgcatattca gcgtattgct taactatcac ccttacttgc tctgatattt cccaatccac
     3001 agacttagcg ttcttatttt ttggctcaag aaattgcata ggaaaatgca cccctttttg
     3061 cttagtaaat tgtaaccatt ttaacacacc aatttgcatt cctacaataa ttatttcacg
     3121 ttcgcatttt tatatacatt ttaacttaca ttttgtggtg taaattaaca acaaacgttg
     3181 atttaacaat aaaaaacgag tatcaaattc agttgcaatt tgatactcat ttaggtttac
     3241 attttcactt tttgtactcc aaaactgaac tacttaa
//
LOCUS       DANKEF010000291         2789 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.153, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000291 DANKEF010000000
VERSION     DANKEF010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.153"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            460..933
                     /gene="dfrF"
                     /locus_tag="PWZ48_003200"
     CDS             460..933
                     /gene="dfrF"
                     /locus_tag="PWZ48_003200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrF"
                     /protein_id="HDL2262750.1"
                     /translation="MIGLIVARSKNNVIGKNGNIPWKIKGEQKQFRELTTGNVVIMGR
                     KSYEEIGHPLPNRMNIVVSTTTEYQGDNLVSVKSLEDALLLAKGRDVYISGGYGLFKE
                     ALQIVDKMYITEVDLNIEDGDTFFPEFDINDFEVLIGETLGEEVKYTRTFYVRKK"
     gene            1060..1716
                     /locus_tag="PWZ48_003201"
     CDS             1060..1716
                     /locus_tag="PWZ48_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HDL2262751.1"
                     /translation="MNKEWSELNKTMQAQIKKKDTYKRGIDTLLTLRSQLIQTLVSFK
                     EELCREDFNSIPFINADGYHSKTIAYSIWHIFRIEDIVVHTVINEDEQVFFAGNYQER
                     INSPIITTGNELMKQQIADFSKQLNLEELYLYIFEVWESTEKMLERLSYDELKRKIPK
                     ERKGYLESLNVVNDNEKAIWLIDYWCNKDICGLIQMPFSRHWIMHTEACLRIKNKIHS
                     "
     gene            1898..2428
                     /locus_tag="PWZ48_003202"
     CDS             1898..2428
                     /locus_tag="PWZ48_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HDL2262752.1"
                     /translation="MRKVVLFIAMSLDGYIADGNGGVAWLNGHGNDNENIDTYTEFTK
                     DIDTVLMGWNTSHQVVTELSPQEWVYNKFTTYVLTHKECNSSQQIHFTSENPVLLLER
                     LKQEAGKDIWICGGANLVQQLVSKNMIDKYYISVIPTLLGNGVRLLGNIDREIKLRLC
                     KTQTYNGITDLIYMRR"
BASE COUNT         1014 a          363 c          587 g          825 t
ORIGIN      
        1 gcgcagcaga gatggcaacg gtgaaaggat gggaaaaagg tgtttcccaa acaaaaagat
       61 attaagaaac agcccatgtt tacctgcttg caaacaaaag aatattgcta ctagactaag
      121 agatactaca taagtgtgtt ggtatctctt ttttgttgcc gtgaaacagc agactatttc
      181 atttctgctt gattacctta aaacttatta gtctatggtt tgtcaagggc ttgttgcgtg
      241 agaaatgctg aatatcaccc tttacaaaac ataggctgat aagtaacttt tcaaaagcag
      301 aaaagaaatg gcaatgctta taacaatatg atataatgca gatgaagaca aaaaaggaat
      361 ttgtcgagct ggttagttcg agataatttt gagtttgtta agatgtggtg cacttgatgg
      421 aatcacaggt acgacatcat attatggagg atgaaataga tgataggttt gattgttgcg
      481 aggtcaaaga ataatgttat aggcaagaat ggtaatatac catggaaaat aaagggagaa
      541 caaaagcaat ttagagagtt aacaacgggt aatgtggtta ttatggggcg aaagtcttat
      601 gaagaaatcg gtcatccgtt gcctaataga atgaatattg ttgtttccac cacaacagag
      661 tatcaaggag ataatttagt ttcagttaaa tcattagaag atgcattatt attggctaaa
      721 ggacgagatg tatacatatc tggtggatat ggactattta aggaagcttt gcaaatagta
      781 gataaaatgt atatcacaga agtagattta aatattgaag atggagatac attctttcca
      841 gaatttgata tcaatgattt tgaagttttg ataggggaaa cacttggtga ggaagtgaaa
      901 tatacgagaa cattttatgt aaggaaaaaa tgaattgagt aaattttgga tttagggtgt
      961 tttcataaat atattgcttt ttatgcatat ataaattgtt gtaagacaaa ttccagtttg
     1021 tatggggcga taaaatcaga atttgagaga ggattgaata tgaataagga atggtctgaa
     1081 cttaataaaa caatgcaggc acaaataaaa aagaaagata cttataagag gggtattgat
     1141 actttactta ctttacgaag tcagttaata caaaccttag tatctttcaa agaagaatta
     1201 tgcagagaag attttaactc aatccccttc ataaacgctg atggttatca tagtaagacg
     1261 attgcttatt ctatttggca tattttccgc attgaagata tagttgtgca tacagtgata
     1321 aatgaagatg aacaagtatt ttttgcaggc aattatcaag agcgtatcaa ttcacctatc
     1381 attacaacag gaaatgaact catgaaacag cagattgcag acttttcaaa acagcttaat
     1441 ctggaagaat tatatttata tattttcgaa gtatgggaaa gcactgagaa aatgcttgaa
     1501 cggttatctt atgatgaatt gaaaagaaaa ataccgaaag aaagaaaagg atacttagaa
     1561 tcgttgaatg tagtaaacga caatgaaaaa gcaatttggc tgattgatta ttggtgtaat
     1621 aaagatattt gtggactaat tcaaatgccg ttttcaagac attggattat gcacacagaa
     1681 gcctgtttgc gtataaagaa caagatacat tcataggcaa aaatttcagt ttgtagaact
     1741 aaggaaattg gaatttaagg aggaataaag tatggttagt gattttataa gagcagcatt
     1801 tccatggatt ttaatgggtt tatttgtagc aatcagttgt tcccttatga gtaaaaaaga
     1861 gaaataaatt cctgtttgta tttataaaag agaaaaaatg agaaaagtag ttttatttat
     1921 tgccatgagc cttgacggat atattgcgga cggtaatggt ggagtagctt ggctaaatgg
     1981 tcatggaaat gacaatgaaa acatcgacac ttatacagaa tttaccaaag atatagatac
     2041 tgtcttaatg gggtggaata cttctcatca ggttgttact gaactttctc cgcaagaatg
     2101 ggtatataat aaatttacaa cttatgtatt aacacataaa gagtgcaatt cttcccaaca
     2161 aattcatttt acaagtgaaa atcctgtttt attattggaa cggctaaaac aagaagcagg
     2221 aaaggatatt tggatttgtg gcggagcaaa tcttgttcag cagttggtaa gcaaaaatat
     2281 gattgataaa tattacattt ctgttattcc tactctgtta ggaaatgggg ttcgtctttt
     2341 gggcaacata gacagggaaa taaagttaag gctttgtaaa acacagactt ataacggaat
     2401 aaccgatttg atttatatgc gtagataact agagagaaac agaaaagctg ttaaaccgac
     2461 ggagcgatcc gaccacgttg gcgggaaaat ccttaagatt tttccgccat gcttgcaagg
     2521 gggcggttcg cttcggcggt tcggcgagct tgcgagccgt tcaaagcccc cgttatctaa
     2581 caaggggggc acaaaaaaca agaaacacta tacactaaca agcggaaaac gctgtattta
     2641 taaggcaaaa ccccattttt acagtagtga tataaattgt acctatatca ctatgaaaat
     2701 tgaatagtaa atatgagaaa gacagttgat ttcaaaaaat aagaaattga ggctctttgt
     2761 caataaggac tgatgggttg tacaaaatc
//
LOCUS       DANKEF010000292         2421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.163, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000292 DANKEF010000000
VERSION     DANKEF010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.163"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            206..1174
                     /locus_tag="PWZ48_003203"
     CDS             206..1174
                     /locus_tag="PWZ48_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2262753.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1167..2198
                     /gene="vanA"
                     /locus_tag="PWZ48_003204"
     CDS             1167..2198
                     /gene="vanA"
                     /locus_tag="PWZ48_003204"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2262754.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2204..>2421
                     /locus_tag="PWZ48_003205"
     CDS             2204..>2421
                     /locus_tag="PWZ48_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HDL2262755.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRP"
BASE COUNT          709 a          461 c          598 g          653 t
ORIGIN      
        1 ataatttttt aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta
       61 atattaagaa acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt
      121 cgttcttcag agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct
      181 ttcacagaaa gggttggtct taattatgaa taacatcggc attactgttt atggatgtga
      241 gcaggatgag gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat
      301 aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag
      361 tgtgggacat aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt
      421 gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag
      481 aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat
      541 gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca
      601 tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt
      661 gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt
      721 gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct
      781 gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat
      841 cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg
      901 tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc
      961 cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc
     1021 aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca
     1081 tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg
     1141 tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg
     1201 gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta
     1261 ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt
     1321 gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg
     1381 ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg
     1441 atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt
     1501 ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg
     1561 acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg
     1621 ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc
     1681 cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact
     1741 acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg
     1801 gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagctgtt ggcgaggtgg
     1861 accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag
     1921 gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga
     1981 tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg
     2041 atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt
     2101 tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac
     2161 tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac
     2221 ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa
     2281 tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt
     2341 ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct
     2401 atgggacggt taccgtccta a
//
LOCUS       DANKEF010000293         2421 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.164, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000293 DANKEF010000000
VERSION     DANKEF010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.164"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            206..1174
                     /locus_tag="PWZ48_003206"
     CDS             206..1174
                     /locus_tag="PWZ48_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HDL2262756.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1167..2198
                     /gene="vanA"
                     /locus_tag="PWZ48_003207"
     CDS             1167..2198
                     /gene="vanA"
                     /locus_tag="PWZ48_003207"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HDL2262757.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2204..>2421
                     /locus_tag="PWZ48_003208"
     CDS             2204..>2421
                     /locus_tag="PWZ48_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HDL2262758.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRP"
BASE COUNT          709 a          460 c          598 g          654 t
ORIGIN      
        1 ataatttttt aggaaaatct caaggttatc tttacttttt cttaggaaat taacaattta
       61 atattaagaa acggctcgtt cttacacggt agacttaata ccgtaagaac gagccgtttt
      121 cgttcttcag agaaagattt gacaagatta ccattggcat ccccgtttta tttggtgcct
      181 ttcacagaaa gggttggtct taattatgaa taacatcggc attactgttt atggatgtga
      241 gcaggatgag gcagatgcat tccatgctct ttcgcctcgc tttggcgtta tggcaacgat
      301 aattaacgcc aacgtgtcgg aatccaacgc caaatccgcg cctttcaatc aatgtatcag
      361 tgtgggacat aaatcagaga tttccgcctc tattcttctt gcgctgaaga gagccggtgt
      421 gaaatatatt tctacccgaa gcatcggctg caatcatata gatacaactg ctgctaagag
      481 aatgggcatc actgtcgaca atgtggcgta ctcgccggat agcgttgccg attatactat
      541 gatgctaatt cttatggcag tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca
      601 tgatttcagg ttggacagcg accgtggcaa ggtactcagc gacatgacag ttggtgtggt
      661 gggaacgggc cagataggca aagcggttat tgagcggctg cgaggatttg gatgtaaagt
      721 gttggcttat agtcgcagcc gaagtataga ggtaaactat gtaccgtttg atgagttgct
      781 gcaaaatagc gatatcgtta cgcttcatgt gccgctcaat acggatacgc actatattat
      841 cagccacgaa caaatacaga gaatgaagca aggagcattt cttatcaata ctgggcgcgg
      901 tccacttgta gatacctatg agttggttaa agcattagaa aacgggaaac tgggcggtgc
      961 cgcattggat gtattggaag gagaggaaga gtttttctac tctgattgca cccaaaaacc
     1021 aattgataat caatttttac ttaaacttca aagaatgcct aacgtgataa tcacaccgca
     1081 tacggcctat tataccgagc aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg
     1141 tttggatttt gaaaggagac aggagcatga atagaataaa agttgcaata ctgtttgggg
     1201 gttgctcaga ggagcatgac gtatcggtaa aatctgcaat agagatagcc gctaacatta
     1261 ataaagaaaa atacgagccg ttatacattg gaattacgaa atctggtgta tggaaaatgt
     1321 gcgaaaaacc ttgcgcggaa tgggaaaacg acaattgcta ttcagctgta ctctcgccgg
     1381 ataaaaaaat gcacggatta cttgttaaaa agaaccatga atatgaaatc aaccatgttg
     1441 atgtagcatt ttcagctttg catggcaagt caggtgaaga tggatccata caaggtctgt
     1501 ttgaattgtc cggtatccct tttgtaggct gcgatattca aagctcagca atttgtatgg
     1561 acaaatcgtt gacatacatc gttgcgaaaa atgctgggat agctactccc gccttttggg
     1621 ttattaataa agatgatagg ccggtggcag ctacgtttac ctatcctgtt tttgttaagc
     1681 cggcgcgttc aggctcatcc ttcggtgtga aaaaagtcaa tagcgcggac gaattggact
     1741 acgcaattga atcggcaaga caatatgaca gcaaaatctt aattgagcag gctgtttcgg
     1801 gctgtgaggt cggttgtgcg gtattgggaa acagtgccgc gttagttgtt ggcgaggtgg
     1861 accaaatcag gctgcagtac ggaatctttc gtattcatca ggaagtcgag ccggaaaaag
     1921 gctctgaaaa cgcagttata accgttcccg cagacctttc agcagaggag cgaggacgga
     1981 tacaggaaac ggcaaaaaaa atatataaag cgctcggctg tagaggtcta gcccgtgtgg
     2041 atatgttttt acaagataac ggccgcattg tactgaacga agtcaatact ctgcccggtt
     2101 tcacgtcata cagtcgttat ccccgtatga tggccgctgc aggtattgca cttcccgaac
     2161 tgattgaccg cttgatcgta ttagcgttaa aggggtgata agcatggaaa taggatttac
     2221 ttttttagat gaaatagtac acggtgttcg ttgggacgct aaatatgcca cttgggataa
     2281 tttcaccgga aaaccggttg acggttatga agtaaatcgc attgtaggga catacgagtt
     2341 ggctgaatcg cttttgaagg caaaagaact ggctgctacc caagggtacg gattgcttct
     2401 atgggacggt taccgtccta a
//
LOCUS       DANKEF010000294         2323 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.166, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000294 DANKEF010000000
VERSION     DANKEF010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.166"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            complement(54..1157)
                     /locus_tag="PWZ48_003209"
     CDS             complement(54..1157)
                     /locus_tag="PWZ48_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HDL2262759.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            1710..2258
                     /gene="aac(6')"
                     /locus_tag="PWZ48_003210"
     CDS             1710..2258
                     /gene="aac(6')"
                     /locus_tag="PWZ48_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HDL2262760.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT          736 a          410 c          455 g          722 t
ORIGIN      
        1 agaaatgtcc tcaatttgat gacagcctgc ttgatggggg ttttagcaat tatttaactg
       61 attttcttat atccttgctg gctttcttgt gtagcatgat aagtttctac gtcaagaatg
      121 gcctgtttaa atttttctcg ggtgatttca taaggcacat gtttaatatc atttgttgcc
      181 atggctttat ctaagattgc ttctaaatcg gtcgtgacat tgagattcat gtcggttaac
      241 gatactggaa gttggttctt tttcataaac acatagattt tatcaaacaa atcgaactgc
      301 tgatccatca tcaataaggt cagtacacca taactgacag atgctccgtg aaggtatccc
      361 tccatttttt ctagaacagt cgtaccgtaa tagaatgagt gtgccatagc agaattatag
      421 ttattgttaa ctaaaacaga aaccaatccg gtagtaacaa tgatatccga aacgacttgt
      481 tcaattgcat atgagggacg atcgtcggca ctatctttca atgcttgcgt accataagtc
      541 aagagacgat caatgcacat ttttccgatt tggattccta atctatccgt gtagcctaga
      601 tcatcgccac gagcagaaaa agtagattcg acttctttgg ataaagcatc gccaatacct
      661 gcccataagt atttatcagg tgcttcggca ataattgctg tatcaatgaa ggtgtgtaca
      721 ggtggttttg aaggaaagaa gagtccgtcc attacgcctt cttcgttata gatgacacat
      781 accgcagtga cgggcgaaca gttcgaacca atagtgggaa aggtgaagac tggtttagct
      841 agtttttcac caatgacttt tacagtatcg caagcacggc caccaccgac tgcaaacagg
      901 acgtctgctt tttggacact ttcatgctgg atcaactgtt caatatttcc ataagttgaa
      961 ttacccccgt accagagact ggcgacaacc tctatttcct gtgtcaaagc tacttttatt
     1021 ttgttttctg cctttgaaag tgcagtcttc cctccaatca gcactacttt tgtcccatat
     1081 tctttgacga ttccatttgc tttttcatac gcatctgttc caatggtata atttggtaag
     1141 aaaacacttt gactcatatt catttccttc tttcttcgtt gaaacgggtt aaaattttaa
     1201 agagtgatcg atctgactgc ggctgtagat ggagtagagg tgaacctaag acaggataag
     1261 tgatgaataa ggtggcatga acaccttgaa atcatcaata agtatcctaa aaacgaacaa
     1321 gtacaaaaac attgaaaacg acaataatga atttttgttc ctcttagtat ttctaccgat
     1381 tccctttgct tttttgtaat tatctaaaaa ttctaaaaat taagattatt atatgaaaac
     1441 aggttttatt tgtcaattca taattggttt tttttaaagc aaaagataat ctatatcatg
     1501 atgaacaaac agagtttttt cctccaattg atggctttcc tgatctttcc tattttgaat
     1561 aaagtgtttc tgtataattt ttttatttta taagtaagat aaataatgaa agaatagaga
     1621 tactgattgg tagctaaaga tttttgagcg agaacatttc gttttttcaa aaggactgtt
     1681 agaataagca agaacaaggg ggaagacgta tgataatcag tgaatttgac cgtaataatc
     1741 cagtattgaa agatcagctt tctgatttac tgagactgac ttggccggaa gaatatggag
     1801 acagctcggc agaagaagta gaagaaatga tgaatccaga acgaatcgcg gtagcagcgg
     1861 tagaccaaga tgagttagta ggatttattg gtgcaatccc tcaatacggt atcacaggtt
     1921 gggaattgca tccattagtt gtagaaagct cccgacgaaa gaaccaaata ggtactcgat
     1981 tagtcaatta cttagaaaaa gaagtagctt ccagaggagg aatcacgatt tatttaggta
     2041 cggatgattt agaccatgga acaacgttaa gtcaaacgga cctgtatgag catacatttg
     2101 ataaagtggc ttccatccag aaccttcgtg aacatccgta tgaattctat gaaaaattag
     2161 gttataaaat cgtaggtgtc ttaccaaatg caaatggctg ggacaaaccg gatatttgga
     2221 tggcaaaaac gattattcct cgaccaaatt ctcaataata aaaagaggtt gtgacagaag
     2281 taatcagctt caagaaataa gaaggaattt acgaaaatta ctt
//
LOCUS       DANKEF010000295         2292 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.168, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000295 DANKEF010000000
VERSION     DANKEF010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.168"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..685
                     /locus_tag="PWZ48_003211"
     CDS             <1..685
                     /locus_tag="PWZ48_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HDL2262761.1"
                     /translation="QEKVYIEYDKVKADTWDRRNMRVEFNPNKLTHEEMIWLKQNIID
                     YMEDDGFTRLDLAFDFEDDLSDYYAMTDKAVKKTVFYGRNGKPETKYFGVRDSDRFIR
                     IYNKKQERKDNADVEVVSEHLWRVEVELKRDMVDYWNDCFNDLHILKPAWATLEKINE
                     QAMVYTLLHEESMWGKLSKNTKTKFKKLIREISPIDLTELMKSTLKANEKQLQKQIDF
                     WQREFRFWK"
     gene            815..1462
                     /locus_tag="PWZ48_003212"
     CDS             815..1462
                     /locus_tag="PWZ48_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-7 chloramphenicol O-acetyltransferase"
                     /protein_id="HDL2262762.1"
                     /translation="MTFNIIELENWDRKEYFEHYFNQQTTYSITKEIDITLFKDMIKK
                     KGYEIYPSLIYAIMEVVNKNKVFRTGINSENKLGYWDKLNPLYTVFNKQTEKFTNIWT
                     ESDKNFISFYNNYKNDLLEYKDKEEMFPKKPIPENTIPISMIPWIDFSSFNLNIGNNS
                     SFLLPIITIGKFYSENNKIYIPVALQLHHSVCDGYHASLFMNEFQDIIHRVDDWI"
     gene            complement(2155..2292)
                     /locus_tag="PWZ48_003213"
                     /pseudo
     CDS             complement(2155..2292)
                     /locus_tag="PWZ48_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
BASE COUNT          842 a          258 c          391 g          801 t
ORIGIN      
        1 tcaagaaaaa gtttatatcg aatatgacaa agtaaaagca gatacttggg atagacgtaa
       61 tatgcgtgtt gaatttaatc ccaataaact cacacatgaa gaaatgattt ggttaaaaca
      121 aaatattatc gactacatgg aagatgacgg ttttacaaga ttagacttag cttttgattt
      181 tgaagatgat ttgagcgatt actatgcaat gactgataaa gcagttaaga aaactgtttt
      241 ttatggtcgt aatggcaagc cagaaacaaa atattttggt gtccgtgata gtgatagatt
      301 tattagaatt tataataaaa aacaagaacg taaagataac gcagatgttg aagttgtgtc
      361 tgaacattta tggcgtgtag aagttgaatt aaaaagagat atggttgatt actggaatga
      421 ttgttttaat gatttacaca tcttgaaacc tgcgtgggct actttagaaa aaattaatga
      481 gcaagctatg gtttatactt tgttgcatga agaaagtatg tggggaaagc taagtaagaa
      541 tactaagact aaatttaaaa aattgattag agaaatatct ccaattgatt taacggaatt
      601 aatgaaatcg actttaaaag cgaacgaaaa acaattgcaa aagcagattg atttttggca
      661 acgtgaattt aggttttgga agtaaaataa gttttatttg ataaaaattg ctaattcagt
      721 ataattaata tttacgaggt gacataacgt atgaaaaaat cagaggatta ttcctcctaa
      781 atataaaaat ttaaaattta ggaggaagtt atatatgact tttaatatta ttgaattaga
      841 aaattgggat agaaaagaat attttgaaca ctattttaat cagcaaacta cttatagcat
      901 tactaaagaa attgatatta ctttgtttaa agatatgata aaaaagaaag gatatgaaat
      961 ttatccctct ttaatttatg caattatgga agttgtaaat aaaaataaag tgtttagaac
     1021 aggaattaat agtgagaata aattaggtta ttgggataag ttaaatcctt tgtatacagt
     1081 ttttaataag caaactgaaa aatttactaa catttggact gaatctgata aaaacttcat
     1141 ttctttttat aataattata aaaatgactt gcttgaatat aaagataaag aagaaatgtt
     1201 tcctaaaaaa ccgatacctg aaaacaccat accgatttca atgattcctt ggattgattt
     1261 tagttcattt aatttaaata ttggtaacaa tagcagcttt ttattgccta ttattacgat
     1321 aggtaaattt tatagtgaga ataataaaat ttatatacca gttgctctgc aacttcatca
     1381 ttctgtatgt gatggttacc atgcttcact atttatgaat gaatttcaag atataattca
     1441 tagggtagat gattggattt agtttttaga ttttgaaagt gaatttaatt ttatacacgt
     1501 aagtgatcat aaaatttatg aacgtataac aaccacattt tttggttgct tgtggttttg
     1561 attttgaatt tggttttgaa cttatggact gatttattca gtccattttt tgtgcttgca
     1621 caaaaactag cctcgcagag cacacgcatt aatgacttat gaaacgtagt aaataagtct
     1681 agtgtgttat actttacttg gaagatgcac cgaataaaaa atattgaaga acaactagca
     1741 aaagatttta aagagttatt ttattttaag tctttataac atgagtgaag cgaattttta
     1801 aatttcgata gaaattttta catcaaaaag ccccctgtca aaattgacga agggggtttt
     1861 ttggcgcacg cttttcgtta gaaatataca agattgaaaa tcgtgtataa gtgcgccctt
     1921 tgttttgaac ttagcacgtt acatcaattt tttaaaatga tgtataagtg cgccctttta
     1981 aattttgagt gattatattt tttgagttag aaaaagggat tgggaaaatt tcccaaaata
     2041 atttaaaaaa taagcaaaaa ttttcgatag agaatgtgct attttttgtc aaaggtgtat
     2101 accttgactg tgcttgctgt tacattaagt ttatttttaa gttattaaaa aagaaatagc
     2161 ttttaaagtt tggctcgctg tcgctttata aagctgattg acttttgatt gcaaactact
     2221 taaagaaaac aaactcggac tattcgtttt cttctctttg gtttgaacat cagcaattat
     2281 cccctcttga tt
//
LOCUS       DANKEF010000296         2022 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.174, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000296 DANKEF010000000
VERSION     DANKEF010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.174"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            221..1132
                     /locus_tag="PWZ48_003214"
     CDS             221..1132
                     /locus_tag="PWZ48_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HDL2262763.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1285..1770
                     /gene="vanZ"
                     /locus_tag="PWZ48_003215"
     CDS             1285..1770
                     /gene="vanZ"
                     /locus_tag="PWZ48_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HDL2262764.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
BASE COUNT          709 a          290 c          390 g          633 t
ORIGIN      
        1 cccattgtca acaggcagtt cagcctcgtt aaattcagca tgggtatcac ttatgaaaat
       61 tcatctacat tggtgataat agtaaatcca gtagggcgaa ataattgact gtaatttacg
      121 gggcaaaacg gcacaatctc aaacgagatt gtgccgttta aggggaagat tctagaaata
      181 tttcatactt ccaactatat agttaaggag gagactgaaa atgaagaagt tgtttttttt
      241 attgttattg ttattcttaa tatacttagg ttatgactac gttaatgaag cactgttttc
      301 tcaggaaaaa gtcgaatttc aaaattatga tcaaaatccc aaagaacatt tagaaaatag
      361 tgggacttct gaaaataccc aagagaaaac aattacagaa gaacaggttt atcaaggaaa
      421 tctgctatta atcaatagta aatatcctgt tcgccaagaa agtgtgaagt cagatatcgt
      481 gaatttatct aaacatgacg aattaataaa tggatacggg ttgcttgata gtaatattta
      541 tatgtcaaaa gaaatagcac aaaaattttc agagatggtc aatgatgctg taaagggtgg
      601 cgttagtcat tttattatta atagtggcta tcgagacttt gatgagcaaa gtgtgcttta
      661 ccaagaaatg ggggctgagt atgccttacc agcaggttat agtgagcata attcaggttt
      721 atcactagat gtaggatcaa gcttgacgaa aatggaacga gcccctgaag gaaagtggat
      781 agaagaaaat gcttggaaat acgggttcat tttacgttat ccagaggaca aaacagagtt
      841 aacaggaatt caatatgaac catggcatat tcgctatgtt ggtttaccac atagtgcgat
      901 tatgaaagaa aagaatttcg ttctcgagga atatatggat tacctaaaag aagaaaaaac
      961 catttctgtt agtgtaaatg gggaaaaata tgagatcttt tattatcctg ttactaaaaa
     1021 taccaccatt catgtgccga ctaatcttcg ttatgagata tcaggaaaca atatagacgg
     1081 tgtaattgtg acagtgtttc ccggatcaac acatactaat tcaaggaggt aaggatggcg
     1141 gaatgaaacc aacgaaatta atgaacagca ttattgtact agcacttttg gggtaacgtt
     1201 agctttttaa tttaaaaccc acgttaacta ggacattgct atactaatga tacaacttaa
     1261 acaaaagaat tagaggaaat tatattggga aaaatattat ctagaggatt gctagcttta
     1321 tatttagtga cactaatctg gttagtgtta ttcaaattac aatacaatat tttatcagta
     1381 tttaattatc atcaaagaag tcttaacttg actccattta ctgctactgg gaatttcaga
     1441 gagatgatag ataatgttat aatctttatt ccatttggct tgcttttgaa tgtcaatttt
     1501 aaagaaatcg gatttttacc taagtttgct tttgtactgg ttttaagtct tacttttgaa
     1561 ataattcaat ttatcttcgc tattggagcg acagacataa cagatgtaat tacaaatact
     1621 gttggaggct ttcttggact gaaattatat ggtttaagca ataagcatat gaatcaaaaa
     1681 aaattagaca gagttattat ttttgtaggt atacttttgc tcgtattatt gctcgtttac
     1741 cgtacccatt taagaataaa ttacgtgtaa gatgtctaaa tcaagcaatc tgatctttca
     1801 tacacataaa gatattgaat gaattggatt agatggaaaa cgggatgtgg ggaaactcgc
     1861 ccgtaggtgt gaagtgaggg gaaaaccggt gataaagtaa aaagcttacc taacactata
     1921 gtaacaaaga aagcccaatt atcaatttta gtgctgagga attggtctct ttaataaatt
     1981 tccttaacgt tgtaaatccg cattttcctg acggtacccc ca
//
LOCUS       DANKEF010000297         1897 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.180, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000297 DANKEF010000000
VERSION     DANKEF010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1897)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1897)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1897
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.180"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            53..748
                     /gene="vanR"
                     /locus_tag="PWZ48_003216"
     CDS             53..748
                     /gene="vanR"
                     /locus_tag="PWZ48_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HDL2262765.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            726..1880
                     /locus_tag="PWZ48_003217"
     CDS             726..1880
                     /locus_tag="PWZ48_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HDL2262766.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT          623 a          363 c          417 g          494 t
ORIGIN      
        1 cctataatct gataaattat taagggcaaa cttatgtgaa agggtgataa ctatgagcga
       61 taaaatactt attgtggatg atgaacatga aattgccgat ttggttgaat tatacttaaa
      121 aaacgagaat tatacggttt tcaaatacta taccgccaaa gaagcattgg aatgtataga
      181 caagtctgag attgaccttg ccatattgga catcatgctt cccggcacaa gcggccttac
      241 tatctgtcaa aaaataaggg acaagcacac ctatccgatt atcatgctga ccgggaaaga
      301 tacagaggta gataaaatta cagggttaac aatcggcgcg gatgattata taacgaagcc
      361 ctttcgccca ctggagttaa ttgctcgggt aaaggcccag ttgcgccgat acaaaaaatt
      421 cagtggagta aaggagcaga acgaaaatgt tatcgtccac tccggccttg tcattaatgt
      481 taacacccat gagtgttatc tgaacgagaa gcagttatcc cttactccca ccgagttttc
      541 aatactgcga atcctctgtg aaaacaaggg gaatgtggtt agctccgagc tgctatttca
      601 tgagatatgg ggcgacgaat atttcagcaa gagcaacaac accatcaccg tgcatatccg
      661 gcatttgcgc gaaaaaatga acgacaccat tgataatccg aaatatataa aaacggtatg
      721 gggggttggt tataaaattg aaaaataaaa aaaacgacta ttccaaacta gaacgaaaac
      781 tttacatgta tatcgttgca attgttgtgg tagcaattgt attcgtgttg tatattcgtt
      841 caatgatccg agggaaactt ggggattgga tcttaagtat tttggaaaac aaatatgact
      901 taaatcacct ggacgcgatg aaattatatc aatattccat acggaacaat atagatatct
      961 ttatttatgt ggcgattgtc attagtattc ttattctatg tcgcgtcatg ctttcaaaat
     1021 tcgcaaaata ctttgacgag ataaataccg gcattgatgt acttattcag aacgaagata
     1081 aacaaattga gctttctgcg gaaatggatg ttatggaaca aaagctcaac acattaaaac
     1141 ggactctgga aaagcgagag caggatgcaa agctggccga acaaagaaaa aatgacgttg
     1201 ttatgtactt ggcgcacgat attaaaacgc cccttacatc cattatcggt tatttgagcc
     1261 tgcttgacga ggctccagac atgccggtag atcaaaaggc aaagtatgtg catatcacgt
     1321 tggacaaagc gtatcgactc gaacagctaa tcgacgagtt ttttgagatt acacggtata
     1381 acctacaaac gataacgcta acaaaaacgc acatagacct atactatatg ctggtgcaga
     1441 tgaccgatga attttatcct cagctttccg cacatggaaa acaggcggtt attcacgccc
     1501 ccgaggatct gaccgtgtcc ggcgaccctg ataaactcgc gagagtcttt aacaacattt
     1561 tgaaaaacgc cgctgcatac agtgaggata acagcatcat tgacattacc gcgggcctct
     1621 ccggggatgt ggtgtcaatc gaattcaaga acactggaag catcccaaaa gataagctag
     1681 ctgccatatt tgaaaagttc tataggctgg acaatgctcg ttcttccgat acgggtggcg
     1741 cgggacttgg attggcgatt gcaaaagaaa ttattgttca gcatggaggg cagatttacg
     1801 cggaaagcaa tgataactat acgacgttta gggtagagct tccagcgatg ccagacttgg
     1861 ttgataaaag gaggtcctaa gagatgtata taatttt
//
LOCUS       DANKEF010000298         1880 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.181, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000298 DANKEF010000000
VERSION     DANKEF010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.181"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            22..1851
                     /gene="tet(M)"
                     /locus_tag="PWZ48_003218"
                     /pseudo
     CDS             22..1851
                     /gene="tet(M)"
                     /locus_tag="PWZ48_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
BASE COUNT          651 a          293 c          392 g          544 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggtt ctgttgcaaa
     1861 gttttaaata aagaataaaa
//
LOCUS       DANKEF010000299         1880 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.182, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000299 DANKEF010000000
VERSION     DANKEF010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.182"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            22..1851
                     /gene="tet(M)"
                     /locus_tag="PWZ48_003219"
                     /pseudo
     CDS             22..1851
                     /gene="tet(M)"
                     /locus_tag="PWZ48_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
BASE COUNT          650 a          293 c          393 g          544 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa agctggaaca
     1621 gaattgttag agccatatct tagttttaaa atttatgcgc cacaggaata tctttcacga
     1681 gcatacaacg atgctcctaa atattgtgcg aacatcgtag acactcaatt gaaaaataat
     1741 gaggtcattc ttagtggaga aatccctgct cggtgtattc aagaatatcg tagtgattta
     1801 actttcttta caaatggacg tagtgtttgt ttaacagagt taaaagggtt ctgttgcaaa
     1861 gttttaaata aagaataaaa
//
LOCUS       DANKEF010000300         1879 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.183, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000300 DANKEF010000000
VERSION     DANKEF010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.183"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            22..1877
                     /gene="tet(M)"
                     /locus_tag="PWZ48_003220"
                     /pseudo
     CDS             22..1877
                     /gene="tet(M)"
                     /locus_tag="PWZ48_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
BASE COUNT          650 a          293 c          392 g          544 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcaggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa gctggaacag
     1621 aattgttaga gccatatctt agttttaaaa tttatgcgcc acaggaatat ctttcacgag
     1681 catacaacga tgctcctaaa tattgtgcga acatcgtaga cactcaattg aaaaataatg
     1741 aggtcattct tagtggagaa atccctgctc ggtgtattca agaatatcgt agtgatttaa
     1801 ctttctttac aaatggacgt agtgtttgtt taacagagtt aaaagggttc tgttgcaaag
     1861 ttttaaataa agaataaaa
//
LOCUS       DANKEF010000301         1879 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.184, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000301 DANKEF010000000
VERSION     DANKEF010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1879
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.184"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            22..1877
                     /gene="tet(M)"
                     /locus_tag="PWZ48_003221"
                     /pseudo
     CDS             22..1877
                     /gene="tet(M)"
                     /locus_tag="PWZ48_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
BASE COUNT          649 a          293 c          393 g          544 t
ORIGIN      
        1 ttttgaatgg aggaaaatca catgaaaatt attaatattg gagttttagc tcatgttgat
       61 gcgggaaaaa ctaccttaac agaaagctta ttatataaca gtggagcgat tacagaatta
      121 ggaagcgtgg acaaaggtac aacgaggacg gataatacgc ttttagaacg tcagagagga
      181 attacaattc agacaggaat aacctctttt cagtgggaaa atacgaaggt gaacatcata
      241 gacacgccag gacatatgga tttcttagca gaagtatatc gttcattatc agttttagat
      301 ggggcaattc tactgatttc tgcaaaagat ggcgtacaag cacaaactcg tatattattt
      361 catgcactta ggaaaatggg gattcccaca atctttttta tcaataagat tgaccaaaat
      421 ggaattgatt tatcaacggt ttatcaggat attaaagaga aactttctgc cgaaattgta
      481 atcaaacaga aggtagaact gtatcctaat atgtgtgtga cgaactttac cgaatctgaa
      541 caatgggata cggtaataga gggaaacgat gaccttttag agaaatatat gtccggtaaa
      601 tcattagaag cattggaact cgaacaagag gaaagcataa gatttcagaa ttgctccttg
      661 taccctgttt atcatggaag cgcaaaaagc aacataggga ttgagcagct tatagaagtg
      721 ataacgaata aattttattc atcaacatac agaaagaagt ctgaactttg cggaaatgtc
      781 ttcaaaattg aatattcgga agaaagacaa cgtcttgcat atgtacgcct ttatggcgga
      841 atcctgcatt tgcgggattc ggttagaata tcggaaaagg aaaaaataaa aattacagaa
      901 atgtatactt caataaatgg tgaattatgt aaaattgata aggcttattc cggggaaatt
      961 gttattttgc aaaatgagtt tttgaagcta aatagtgttc ttggagatac aaagctattg
     1021 ccacagagag agagaattga aaatccgctc cctctgctgc aaacaactgt tgaaccgagc
     1081 aaacctcaac aaagggaaat gttacttgat gcacttttag aaatctccga cagtgacccg
     1141 cttctacaat attatgtgga ttctacgaca catgaaatta tactttcttt cttagggaaa
     1201 gtacaaatgg aagtgattag tgcactgttg caagaaaagt atcatgtgga gatagaacta
     1261 aaagagccta cagtcattta tatggagaga ccgttaaaaa atgcagaata taccattcac
     1321 atcgaagtgc cgccaaatcc tttctgggct tccattggtt tatctgtatc accgcttccg
     1381 ttgggaagtg gaatgcagta tgagagctcg gtttctcttg gatacttaaa tcaatcattt
     1441 caaaatgcag ttatggaagg gatacgctat ggttgcgaac aaggattata tggttggaat
     1501 gtgacggatt gtaaaatctg ttttaagtat ggcttatact atagccctgt tagtacccca
     1561 gcagattttc ggatgcttgc tcctattgta ttggaacaag tcttaaaaaa gctggaacag
     1621 aattgttaga gccatatctt agttttaaaa tttatgcgcc acaggaatat ctttcacgag
     1681 catacaacga tgctcctaaa tattgtgcga acatcgtaga cactcaattg aaaaataatg
     1741 aggtcattct tagtggagaa atccctgctc ggtgtattca agaatatcgt agtgatttaa
     1801 ctttctttac aaatggacgt agtgtttgtt taacagagtt aaaagggttc tgttgcaaag
     1861 ttttaaataa agaataaaa
//
LOCUS       DANKEF010000302         1665 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.191, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000302 DANKEF010000000
VERSION     DANKEF010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1665)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1665)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1665
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.191"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..317
                     /locus_tag="PWZ48_003222"
     CDS             <1..317
                     /locus_tag="PWZ48_003222"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDL2262767.1"
                     /translation="PQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENS
                     HEIAEVIMVLSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQF
                     CK"
     gene            424..1662
                     /locus_tag="PWZ48_003223"
     CDS             424..1662
                     /locus_tag="PWZ48_003223"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDL2262768.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
BASE COUNT          511 a          245 c          287 g          622 t
ORIGIN      
        1 ctcctcaact attaaacaat cctcgttttc ttgctatgca aatagaacaa atgtatgaaa
       61 ttgtagtacc tgaatttgtt gaacccgttt tagaacaagg tatcaaggat aagtctttgg
      121 agattgaaaa ttcacatgaa attgcagaag ttatcatggt attaagtaat gtatggctca
      181 acccattagt tgaaatgacg gataaagagg gtatggaaaa acgttgcaat acattcaatg
      241 cccttttaaa tggaataggc atagacacat tattagacga agaaacaatt tctgcattta
      301 tacagttttg taaataactg tatttgttat ccctatacga tagactgctc tataaaagag
      361 cagtttattt aaaccattat ataaaccgac ggtctgtttg attaaataaa aaggagagat
      421 tttatgcaag cagaaaaaaa actttttaat cgtgatttta tacttgttgt tataggacag
      481 ataatttctt tatttggaaa cgctatccta cgatttgcat taccgtacta tttgttaaaa
      541 gaaactggtt catcagcttt gtttggtatt gctacagcct gctctttttt acctatggtt
      601 attttgtcgc tggtaggtgg tattattgct gacaggacaa ataaaagaaa cattatggtc
      661 atactagatt ttgtaacagc aatcataatt ttagtatttt atttgtcatt gggaaaaatt
      721 cctatcatac ctttgtttat tgttgctttg atgttacttt atggtatttc tggtgcatat
      781 caaccagcgg tacaagcaag tattcctcta ttggtatcat cagataaact taccgtcggt
      841 aatgctataa taaatcaagt aaatacttta gcaaacttta tcggaccagc tataagcgga
      901 gtagtttttc aattttatgg tatcaatccc attttaattg ttagtattat atgttttact
      961 ttttcagcca ttatggaaat ttttatccat attccacacc aaaaaagacc tcgtgaagta
     1021 ggtgtaattg cagttgctaa aaacgattta aaagatagtt ataccttttt gaaaaaggag
     1081 aaacccattt ttatatccgt aatctttttg gtttgcattt tcaatttggt tttaagtgct
     1141 gttttaattg ttggtctgcc tatcttaatt tctgaagttt taaaaatgcc agatacacta
     1201 tatggttttt cacaatcagc attatcatta ggtggtttat gtggtggtat tttaacagct
     1261 cttgttgctt caaagttaaa actaaaaaac ctttatacct tgttacttat ttgttctgtt
     1321 tctgttttac ttatgggaat ttcgctattt ttagaattgc ctgcaatagt ttcctactgg
     1381 gtagttacag tgatgtgctt tgtggctatg ggtgtatcta ctatgtttgt tgttcaaatt
     1441 tacactcttg ttcagacaca aacaccaccg caattagtag gaaagataat ggctacgctg
     1501 gtagcagttg ctatgtgcgg tcaacctatt ggacaaatgg tttacggtat tttattcgat
     1561 gtatttatac aagaaacatg ggttgttatg tttgtagctt ctattgcttc aatcatgatt
     1621 tcaatatatt caaaaagaat ttttcaaaag ttagagatat aatac
//
LOCUS       DANKEF010000303         1641 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.193, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000303 DANKEF010000000
VERSION     DANKEF010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.193"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            86..145
                     /gene="ermCL"
                     /locus_tag="PWZ48_003224"
     CDS             86..145
                     /gene="ermCL"
                     /locus_tag="PWZ48_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002497446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methylase leader peptide ErmCL"
                     /protein_id="HDL2262769.1"
                     /translation="MGIFSIFVINTVHYQPNKK"
     gene            193..927
                     /locus_tag="PWZ48_003225"
     CDS             193..927
                     /locus_tag="PWZ48_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011282421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(T)"
                     /protein_id="HDL2262770.1"
                     /translation="MNKKNIKDSQNFITSKHHINEILRNVHLNTNDNIIEIGSGKGHF
                     TFELAKRCNYVTAIEIDPKLCRITKNKLIEYENFQVINKDILQFKFPKNKSYKIYGNI
                     PYNISTDIIRKIVFESTATESYLIVEYGFAKRLLNTNRSLALFLMTEVDISILSKIPR
                     EYFHPKPRVNSSLIVLKRHPSKISLKDRKQYENFVMKWVNKEYIKLFSKNQFYQALKY
                     ARIDDLNNISFEQFLSLFNSYKLFNR"
BASE COUNT          611 a          258 c          222 g          550 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccag cttgtgctat aattattcta
       61 atttcataaa aaggagaatt aatatatggg catttttagt atttttgtaa tcaacacagt
      121 tcattatcaa ccaaacaaaa aataagtggt tataatggat tgttaatata aacctcatag
      181 gagtgggtta taatgaacaa aaaaaatata aaagatagtc aaaactttat tacttcaaag
      241 catcatataa atgaaatttt gagaaatgta catttaaata caaatgataa tattattgag
      301 attggttcag ggaaaggtca tttcacgttt gaattagcta aaaggtgtaa ttatgtaacc
      361 gccattgaaa tagatcctaa attatgtagg ataactaaaa acaaacttat tgaatatgag
      421 aacttccagg ttatcaataa agatatttta caatttaagt ttcctaaaaa taagtcatat
      481 aagatatatg gaaatatacc ctacaatata agtacagata taattcgaaa aattgttttt
      541 gaaagcacag ctacagaaag ttatttaata gtggaatatg gatttgctaa aaggttgcta
      601 aatacaaatc gttcactagc actattttta atgacagaag ttgatatatc catattaagt
      661 aaaatcccta gagaatactt tcatccaaaa cctagagtta atagctcgtt aattgtatta
      721 aaaagacacc cttcaaaaat atcactcaaa gatagaaaac aatatgaaaa ttttgttatg
      781 aaatgggtta acaaagaata cataaaacta ttttccaaaa accaatttta tcaagcctta
      841 aaatatgcaa gaattgacga tttaaacaat attagctttg aacaattctt gtctcttttc
      901 aatagctata aattatttaa tagataagtt aaggggtgca taaactgcat cccttaactt
      961 atcttatttt gcttaaacaa tcttggcccg taaactgcat aactcatatg taaatcgctc
     1021 ctttttaggt ggcacaaatg tgaggcattt tcactatttc cggcaattac ttccatgtaa
     1081 agtataaccc actatacttt gcaagcaaag tgtgggctcg ccgaggggtc ttgcagacgg
     1141 ctctttcggc tcttttgagc cgagtatcag cgcaccctta tggtgcaatt agctgatacc
     1201 tgtttgacga tttgctgccc taccttttcg gtaggtatgc aaacctcaaa ccaaaataaa
     1261 aacccccttc gacttcgtca gggggttttt atttatataa atctccgtaa ataatcgcga
     1321 tttaataaca tcttcaattt tatttttttg cgtattttag aaatgttttc actaaaaaat
     1381 atattataca aaaaatacag taatcgatta taattctttg ccagttaaat atcatatcgt
     1441 tattaaaata tcggaaaaaa atatcaacaa caataaatgc aaaagaagac cataaaaatt
     1501 gtttattact catacatact ctccatttct atcattttta aatttaccag tcatctctca
     1561 aaaatctcct agctattttt cttttttaac cacataccgt caatggttct gttgcaaagt
     1621 tttaaatcta ctatcaaata a
//
LOCUS       DANKEF010000304         1396 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.207, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000304 DANKEF010000000
VERSION     DANKEF010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.207"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..1311
                     /locus_tag="PWZ48_003226"
     CDS             <1..1311
                     /locus_tag="PWZ48_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HDL2262771.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          600 a          104 c          236 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat gaaaaataat
     1381 aaaggaagtg agtcaa
//
LOCUS       DANKEF010000305         1124 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.222, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000305 DANKEF010000000
VERSION     DANKEF010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1124
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.222"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            24..416
                     /locus_tag="PWZ48_003227"
     CDS             24..416
                     /locus_tag="PWZ48_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDL2262772.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1124
                     /gene="aac(6')-Ie"
                     /locus_tag="PWZ48_003228"
     CDS             417..>1124
                     /gene="aac(6')-Ie"
                     /locus_tag="PWZ48_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ie"
                     /protein_id="HDL2262773.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKG"
BASE COUNT          456 a           88 c          195 g          385 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggt
//
LOCUS       DANKEF010000306          991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.237, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000306 DANKEF010000000
VERSION     DANKEF010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.237"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..352
                     /locus_tag="PWZ48_003229"
     CDS             <1..352
                     /locus_tag="PWZ48_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL2262774.1"
                     /translation="VGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGR
                     IQETAKKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIAL
                     PELIDRLIVLALKG"
     gene            358..966
                     /locus_tag="PWZ48_003230"
     CDS             358..966
                     /locus_tag="PWZ48_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL2262775.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          289 a          203 c          247 g          252 t
ORIGIN      
        1 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
       61 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
      121 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
      181 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
      241 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
      301 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
      361 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
      421 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
      481 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
      541 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaactg ttttatgcaa
      601 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
      661 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
      721 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
      781 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
      841 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
      901 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
      961 aaataaactt ttaaccgttg cacggacaaa c
//
LOCUS       DANKEF010000307          991 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.238, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000307 DANKEF010000000
VERSION     DANKEF010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..991
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.238"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            <1..352
                     /locus_tag="PWZ48_003231"
     CDS             <1..352
                     /locus_tag="PWZ48_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HDL2262776.1"
                     /translation="VGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGR
                     IQETAKKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIAL
                     PELIDRLIVLALKG"
     gene            358..966
                     /locus_tag="PWZ48_003232"
     CDS             358..966
                     /locus_tag="PWZ48_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HDL2262777.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          289 a          203 c          246 g          253 t
ORIGIN      
        1 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
       61 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
      121 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
      181 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
      241 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
      301 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
      361 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
      421 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
      481 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
      541 tacggattgc ttctatggga cggttaccgt cctaatcgtg ctgtaaactg ttttatgcaa
      601 tgggctgcac agccggaaaa taacctgaca aaggaaagtt attatcccaa tattgaccga
      661 actgagatga tttcaaaagg atacgtggct tcaaaatcaa gccatagccg cggcagtgcc
      721 attgatctta cgctttatcg attagacacg ggtgagcttg taccaatggg gagccgattt
      781 gattttatgg atgaacgctc tcatcatgcg gcaaatggaa tatcatgcaa tgaagcgcaa
      841 aatcgcagac gtttgcgctc catcatggaa aacagtgggt ttgaagcata tagcctcgaa
      901 tggtggcact atgtattaag agacgaacca taccccaata gctattttga tttccccgtt
      961 aaataaactt ttaaccgttg cacggacaaa c
//
LOCUS       DANKEF010000308          772 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.253, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000308 DANKEF010000000
VERSION     DANKEF010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.253"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            18..755
                     /gene="erm(B)"
                     /locus_tag="PWZ48_003233"
     CDS             18..755
                     /gene="erm(B)"
                     /locus_tag="PWZ48_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2262778.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          287 a          138 c          120 g          227 t
ORIGIN      
        1 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
       61 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      121 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      181 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      241 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      301 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      361 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      421 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      481 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      541 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      601 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      661 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      721 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
LOCUS       DANKEF010000309          772 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.254, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000309 DANKEF010000000
VERSION     DANKEF010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.254"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            18..755
                     /gene="erm(B)"
                     /locus_tag="PWZ48_003234"
     CDS             18..755
                     /gene="erm(B)"
                     /locus_tag="PWZ48_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2262779.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNIRVTLIHQDILQFQFPNKQRYKIVGSIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          286 a          137 c          121 g          228 t
ORIGIN      
        1 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
       61 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      121 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      181 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      241 ttcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      301 ataaaattgt tgggagtatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      361 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      421 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      481 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      541 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      601 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      661 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      721 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
LOCUS       DANKEF010000310          772 bp    DNA     linear   BCT 22-FEB-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate Enterococcus faecium
            SAMEA111538981-rid17239003.guided.255, whole genome shotgun
            sequence.
ACCESSION   DANKEF010000310 DANKEF010000000
VERSION     DANKEF010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA111538981
            Sequence Read Archive: ERR10688544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (22-FEB-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 22-FEB-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001638294.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001638294.1
            Genome Coverage       :: 126x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 02/22/2023 19:07:52
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,235
            CDSs (total)                      :: 3,167
            Genes (coding)                    :: 3,081
            CDSs (with protein)               :: 3,081
            Genes (RNA)                       :: 68
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2, 2 (5S, 16S, 23S)
            tRNAs                             :: 58
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 86
            CDSs (without protein)            :: 86
            Pseudo Genes (ambiguous residues) :: 0 of 86
            Pseudo Genes (frameshifted)       :: 38 of 86
            Pseudo Genes (incomplete)         :: 42 of 86
            Pseudo Genes (internal stop)      :: 14 of 86
            Pseudo Genes (multiple problems)  :: 8 of 86
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..772
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA111538981-rid17239003.guided.255"
                     /isolate="Enterococcus faecium"
                     /isolation_source="Swab rectum"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="Denmark"
                     /collection_date="2016-04-05"
     gene            18..755
                     /gene="erm(B)"
                     /locus_tag="PWZ48_003235"
     CDS             18..755
                     /gene="erm(B)"
                     /locus_tag="PWZ48_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HDL2262780.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          287 a          137 c          120 g          228 t
ORIGIN      
        1 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
       61 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      121 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      181 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaata
      241 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      301 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      361 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      421 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      481 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      541 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      601 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      661 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
      721 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt tt
//
